LOCUS       DACESX010000001        97239 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.001, whole genome shotgun sequence.
ACCESSION   DACESX010000001 DACESX010000000
VERSION     DACESX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 97239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.001"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            42..1166
                     /locus_tag="IXF88_000001"
     CDS             42..1166
                     /locus_tag="IXF88_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1506765.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            1393..2310
                     /locus_tag="IXF88_000002"
     CDS             1393..2310
                     /locus_tag="IXF88_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1506766.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            2303..3136
                     /locus_tag="IXF88_000003"
     CDS             2303..3136
                     /locus_tag="IXF88_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1506767.1"
                     /translation="MTKQRIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            3219..4337
                     /locus_tag="IXF88_000004"
     CDS             3219..4337
                     /locus_tag="IXF88_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1506768.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            4331..6229
                     /locus_tag="IXF88_000005"
     CDS             4331..6229
                     /locus_tag="IXF88_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1506769.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            6223..7446
                     /locus_tag="IXF88_000006"
     CDS             6223..7446
                     /locus_tag="IXF88_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1506770.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7532..9466)
                     /locus_tag="IXF88_000007"
     CDS             complement(7532..9466)
                     /locus_tag="IXF88_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1506771.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            9692..10348
                     /locus_tag="IXF88_000008"
     CDS             9692..10348
                     /locus_tag="IXF88_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1506772.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(10422..10775)
                     /locus_tag="IXF88_000009"
     CDS             complement(10422..10775)
                     /locus_tag="IXF88_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1506773.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            10967..11896
                     /locus_tag="IXF88_000010"
     CDS             10967..11896
                     /locus_tag="IXF88_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1506774.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            11907..12743
                     /locus_tag="IXF88_000011"
     CDS             11907..12743
                     /locus_tag="IXF88_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1506775.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            12991..13767
                     /locus_tag="IXF88_000012"
     CDS             12991..13767
                     /locus_tag="IXF88_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1506776.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            13943..14686
                     /locus_tag="IXF88_000013"
     CDS             13943..14686
                     /locus_tag="IXF88_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1506777.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            14679..16259
                     /locus_tag="IXF88_000014"
     CDS             14679..16259
                     /locus_tag="IXF88_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1506778.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(16510..16989)
                     /locus_tag="IXF88_000015"
     CDS             complement(16510..16989)
                     /locus_tag="IXF88_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1506779.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(17005..17757)
                     /locus_tag="IXF88_000016"
     CDS             complement(17005..17757)
                     /locus_tag="IXF88_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1506780.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            17874..17944
                     /locus_tag="IXF88_000017"
     tRNA            17874..17944
                     /locus_tag="IXF88_000017"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17906..17908,aa:Gly,seq:ccc)
     gene            18305..19030
                     /locus_tag="IXF88_000018"
     CDS             18305..19030
                     /locus_tag="IXF88_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1506781.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            19285..19551
                     /locus_tag="IXF88_000019"
     CDS             19285..19551
                     /locus_tag="IXF88_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1506782.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            19548..19979
                     /gene="ruvX"
                     /locus_tag="IXF88_000020"
     CDS             19548..19979
                     /gene="ruvX"
                     /locus_tag="IXF88_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1506783.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            20004..20309
                     /locus_tag="IXF88_000021"
     CDS             20004..20309
                     /locus_tag="IXF88_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1506784.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(20390..21535)
                     /locus_tag="IXF88_000022"
     CDS             complement(20390..21535)
                     /locus_tag="IXF88_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1506785.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(21596..22243)
                     /locus_tag="IXF88_000023"
     CDS             complement(21596..22243)
                     /locus_tag="IXF88_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1506786.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            22437..22730
                     /locus_tag="IXF88_000024"
     CDS             22437..22730
                     /locus_tag="IXF88_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506787.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            22774..23085
                     /locus_tag="IXF88_000025"
     CDS             22774..23085
                     /locus_tag="IXF88_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506788.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            23082..23706
                     /locus_tag="IXF88_000026"
                     /pseudo
     CDS             23082..23706
                     /locus_tag="IXF88_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            24006..24368
                     /locus_tag="IXF88_000027"
     CDS             24006..24368
                     /locus_tag="IXF88_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506789.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            24405..25253
                     /locus_tag="IXF88_000028"
     CDS             24405..25253
                     /locus_tag="IXF88_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ1506790.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            25243..26718
                     /locus_tag="IXF88_000029"
     CDS             25243..26718
                     /locus_tag="IXF88_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAQ1506791.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            26984..27391
                     /locus_tag="IXF88_000030"
     CDS             26984..27391
                     /locus_tag="IXF88_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAQ1506792.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVALCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            27394..27609
                     /locus_tag="IXF88_000031"
     CDS             27394..27609
                     /locus_tag="IXF88_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506793.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            27613..27993
                     /locus_tag="IXF88_000032"
     CDS             27613..27993
                     /locus_tag="IXF88_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1506794.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            28127..28282
                     /locus_tag="IXF88_000033"
     CDS             28127..28282
                     /locus_tag="IXF88_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAQ1506795.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            28351..28824
                     /locus_tag="IXF88_000034"
     CDS             28351..28824
                     /locus_tag="IXF88_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ1506796.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            28821..30515
                     /locus_tag="IXF88_000035"
     CDS             28821..30515
                     /locus_tag="IXF88_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1506797.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            30481..30666
                     /locus_tag="IXF88_000036"
     CDS             30481..30666
                     /locus_tag="IXF88_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506798.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            30670..31845
                     /locus_tag="IXF88_000037"
     CDS             30670..31845
                     /locus_tag="IXF88_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1506799.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            31838..33361
                     /locus_tag="IXF88_000038"
     CDS             31838..33361
                     /locus_tag="IXF88_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1506800.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            33417..33701
                     /locus_tag="IXF88_000039"
     CDS             33417..33701
                     /locus_tag="IXF88_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ1506801.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPITVQALVQQMRGAYYANH"
     gene            33688..34023
                     /locus_tag="IXF88_000040"
     CDS             33688..34023
                     /locus_tag="IXF88_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1506802.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            34318..34944
                     /locus_tag="IXF88_000041"
     CDS             34318..34944
                     /locus_tag="IXF88_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506803.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            35686..36523
                     /locus_tag="IXF88_000042"
                     /pseudo
     CDS             35686..36523
                     /locus_tag="IXF88_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            36588..37022
                     /locus_tag="IXF88_000043"
     CDS             36588..37022
                     /locus_tag="IXF88_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1506804.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(37090..38412)
                     /locus_tag="IXF88_000044"
     CDS             complement(37090..38412)
                     /locus_tag="IXF88_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1506805.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(38566..40206)
                     /locus_tag="IXF88_000045"
     CDS             complement(38566..40206)
                     /locus_tag="IXF88_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1506806.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            40536..41006
                     /locus_tag="IXF88_000046"
     CDS             40536..41006
                     /locus_tag="IXF88_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1506807.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            41635..42738
                     /locus_tag="IXF88_000047"
     CDS             41635..42738
                     /locus_tag="IXF88_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1506808.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            43022..43702
                     /locus_tag="IXF88_000048"
     CDS             43022..43702
                     /locus_tag="IXF88_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1506809.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            43792..44826
                     /locus_tag="IXF88_000049"
     CDS             43792..44826
                     /locus_tag="IXF88_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1506810.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            44840..45175
                     /locus_tag="IXF88_000050"
     CDS             44840..45175
                     /locus_tag="IXF88_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506811.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            45159..47639
                     /locus_tag="IXF88_000051"
     CDS             45159..47639
                     /locus_tag="IXF88_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506812.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            47695..49254
                     /locus_tag="IXF88_000052"
     CDS             47695..49254
                     /locus_tag="IXF88_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1506813.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            49251..50615
                     /locus_tag="IXF88_000053"
     CDS             49251..50615
                     /locus_tag="IXF88_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1506814.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLTRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            50786..51856
                     /locus_tag="IXF88_000054"
     CDS             50786..51856
                     /locus_tag="IXF88_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1506815.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            51871..52539
                     /locus_tag="IXF88_000055"
     CDS             51871..52539
                     /locus_tag="IXF88_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1506816.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            52559..53137
                     /locus_tag="IXF88_000056"
     CDS             52559..53137
                     /locus_tag="IXF88_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1506817.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            53540..54451
                     /locus_tag="IXF88_000057"
     CDS             53540..54451
                     /locus_tag="IXF88_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1506818.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(54707..55624)
                     /locus_tag="IXF88_000058"
     CDS             complement(54707..55624)
                     /locus_tag="IXF88_000058"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1506819.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            55826..56263
                     /gene="zapA"
                     /locus_tag="IXF88_000059"
     CDS             55826..56263
                     /gene="zapA"
                     /locus_tag="IXF88_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1506820.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            56321..56869
                     /locus_tag="IXF88_000060"
     CDS             56321..56869
                     /locus_tag="IXF88_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1506821.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            56984..59344
                     /locus_tag="IXF88_000061"
     CDS             56984..59344
                     /locus_tag="IXF88_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1506822.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            59431..59745
                     /gene="trxA"
                     /locus_tag="IXF88_000062"
     CDS             59431..59745
                     /gene="trxA"
                     /locus_tag="IXF88_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1506823.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            59927..61705
                     /gene="uvrC"
                     /locus_tag="IXF88_000063"
     CDS             59927..61705
                     /gene="uvrC"
                     /locus_tag="IXF88_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1506824.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            61850..62749
                     /locus_tag="IXF88_000064"
     CDS             61850..62749
                     /locus_tag="IXF88_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1506825.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            62742..64004
                     /locus_tag="IXF88_000065"
     CDS             62742..64004
                     /locus_tag="IXF88_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1506826.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            64167..66065
                     /locus_tag="IXF88_000066"
     CDS             64167..66065
                     /locus_tag="IXF88_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1506827.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(66364..67083)
                     /locus_tag="IXF88_000067"
     CDS             complement(66364..67083)
                     /locus_tag="IXF88_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1506828.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            67420..67752
                     /locus_tag="IXF88_000068"
     CDS             67420..67752
                     /locus_tag="IXF88_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506829.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            67739..69742
                     /locus_tag="IXF88_000069"
     CDS             67739..69742
                     /locus_tag="IXF88_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1506830.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            69761..70231
                     /locus_tag="IXF88_000070"
     CDS             69761..70231
                     /locus_tag="IXF88_000070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1506831.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            70252..70836
                     /locus_tag="IXF88_000071"
     CDS             70252..70836
                     /locus_tag="IXF88_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1506832.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            70851..71837
                     /locus_tag="IXF88_000072"
     CDS             70851..71837
                     /locus_tag="IXF88_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1506833.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            71827..72138
                     /locus_tag="IXF88_000073"
     CDS             71827..72138
                     /locus_tag="IXF88_000073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1506834.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            72160..73941
                     /locus_tag="IXF88_000074"
     CDS             72160..73941
                     /locus_tag="IXF88_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1506835.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            73934..75310
                     /locus_tag="IXF88_000075"
     CDS             73934..75310
                     /locus_tag="IXF88_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1506836.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            75317..75952
                     /locus_tag="IXF88_000076"
     CDS             75317..75952
                     /locus_tag="IXF88_000076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1506837.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            75982..77337
                     /locus_tag="IXF88_000077"
     CDS             75982..77337
                     /locus_tag="IXF88_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1506838.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(77529..78923)
                     /locus_tag="IXF88_000078"
     CDS             complement(77529..78923)
                     /locus_tag="IXF88_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1506839.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            79015..79986
                     /locus_tag="IXF88_000079"
     CDS             79015..79986
                     /locus_tag="IXF88_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1506840.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            79983..80765
                     /locus_tag="IXF88_000080"
     CDS             79983..80765
                     /locus_tag="IXF88_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ1506841.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(80934..81305)
                     /locus_tag="IXF88_000081"
     CDS             complement(80934..81305)
                     /locus_tag="IXF88_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1506842.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            81439..81882
                     /locus_tag="IXF88_000082"
     CDS             81439..81882
                     /locus_tag="IXF88_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1506843.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(81925..82734)
                     /gene="yidA"
                     /locus_tag="IXF88_000083"
     CDS             complement(81925..82734)
                     /gene="yidA"
                     /locus_tag="IXF88_000083"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1506844.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(82753..84120)
                     /locus_tag="IXF88_000084"
     CDS             complement(82753..84120)
                     /locus_tag="IXF88_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1506845.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(84104..84934)
                     /locus_tag="IXF88_000085"
     CDS             complement(84104..84934)
                     /locus_tag="IXF88_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1506846.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            85097..85534
                     /locus_tag="IXF88_000086"
     CDS             85097..85534
                     /locus_tag="IXF88_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1506847.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            85610..86230
                     /locus_tag="IXF88_000087"
     CDS             85610..86230
                     /locus_tag="IXF88_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1506848.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(86334..87293)
                     /locus_tag="IXF88_000088"
     CDS             complement(86334..87293)
                     /locus_tag="IXF88_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1506849.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            87565..87963
                     /locus_tag="IXF88_000089"
     CDS             87565..87963
                     /locus_tag="IXF88_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1506850.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            88200..88742
                     /locus_tag="IXF88_000090"
     CDS             88200..88742
                     /locus_tag="IXF88_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1506851.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(88825..89376)
                     /gene="lepB"
                     /locus_tag="IXF88_000091"
     CDS             complement(88825..89376)
                     /gene="lepB"
                     /locus_tag="IXF88_000091"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1506852.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(89528..89755)
                     /locus_tag="IXF88_000092"
     CDS             complement(89528..89755)
                     /locus_tag="IXF88_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1506853.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(89752..90270)
                     /gene="msrA"
                     /locus_tag="IXF88_000093"
     CDS             complement(89752..90270)
                     /gene="msrA"
                     /locus_tag="IXF88_000093"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1506854.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(90290..91003)
                     /locus_tag="IXF88_000094"
     CDS             complement(90290..91003)
                     /locus_tag="IXF88_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1506855.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(91006..91902)
                     /locus_tag="IXF88_000095"
     CDS             complement(91006..91902)
                     /locus_tag="IXF88_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1506856.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(92060..92902)
                     /locus_tag="IXF88_000096"
     CDS             complement(92060..92902)
                     /locus_tag="IXF88_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1506857.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            93034..93687
                     /locus_tag="IXF88_000097"
     CDS             93034..93687
                     /locus_tag="IXF88_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1506858.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(93750..94277)
                     /locus_tag="IXF88_000098"
     CDS             complement(93750..94277)
                     /locus_tag="IXF88_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1506859.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(94297..95244)
                     /locus_tag="IXF88_000099"
     CDS             complement(94297..95244)
                     /locus_tag="IXF88_000099"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1506860.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(95256..97142)
                     /locus_tag="IXF88_000100"
     CDS             complement(95256..97142)
                     /locus_tag="IXF88_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1506861.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
BASE COUNT        31448 a        16732 c        19789 g        29270 t
ORIGIN      
        1 agcgttttat ataagaactt gaaaagaaag aagggataga catgaaaaaa agatacaaat
       61 tggtgatagc tttgctaaac attaccctat tattcagctt aagtgcatgt ggaaaaacta
      121 acgaagaagt aacagcaaac atcgcagatc caatgataaa aaaagccact acattagaag
      181 tatatgtcgc aaacggcaaa ctgacaaact atgttgaaaa tgctgcaaaa atctataacc
      241 gcaaaactgg agcaaatatt gacttaaaaa ttgtgaatgt agcttcggga acagcaacag
      301 tacaaatgat caccccaaaa ttggtaacac acgaagatat gccggatatt atctccatgg
      361 cagacacttc agcagcaggt gtgctggaaa aatttcaaga tgctttttat agtgctgatg
      421 aatatggatt ttatgacaaa tatggcgatc agttttatga tcaaaaattg aatattttgc
      481 gtcaacaaac gagtaatgaa acgattattc cttgggctag tgattttacc ccagcacttt
      541 gttattatca accaaaaaaa tttgctgaaa taggtgtaga ttttgaagaa atcagttctt
      601 gggaagaata tatccaggtg gcaaaagcat taaaagaaaa aactggtagt tttggaattg
      661 ccttgccaga atctggggac caagaattgt ttatcaacat gctagcccag cagaatcaat
      721 ctttgcttga tgaagaagga aatatccgtc tgaatactaa agaagcgaaa catgctgcaa
      781 agttaatcaa agaaatgatc gactcagata ttgtacattt ctacggtgca caagatgctg
      841 agaaagcatt tcaagaatct gcaatgtttg ttgctggcgg ctggtatgct acgaatatgt
      901 ctttgaattt tcctgatgca tctgacaaat ggcgcatggc tccactgatt cctttttcaa
      961 aagaaaatcc aggaaaaggt gcagtatcag gaggatcaag tttttatgtc cctaaagatg
     1021 ccaaaaatcc acaagtggca caacaatttc ttacatttat gctgacagat gaagaatgct
     1081 tagccaacgc actggagctg ggcgtggcta catctaactc attggcctat caaacagaag
     1141 cagctgagaa aaagtttgac tactaaggag gacaaaaata ctatgaacta ttgaataaaa
     1201 tcaatgaaaa tacatcaaat actgttttac cagccactta ttcagatgct acgtcttatt
     1261 tgactaccgc tgcatataac tattggcaaa caggtgattt tgaaaattca tatctaaaag
     1321 aagcagaaaa ttttgctcat aagtacggcg taaaattaac tggtgattaa aaaatattag
     1381 gaggacttaa cgatgatgca atcttcgtta ccaagcaaga caataaagaa cagcaagaaa
     1441 aaagcaacag atacgtctca taaaaaaagt gcttatggat tggtcacacc ttttgttatt
     1501 ttctttattg tgacgatttt gattcccatc attatctcgt ttgcttttag ttttatgaaa
     1561 attggctact catttgaatt tgttggattt gacaattatc tagctatctt ccgagatcca
     1621 ttgtttatta aatcttacac gaatgttttg attatcatgg caggatcaat tccaataacg
     1681 atgctattag ctatttactt tgctgtttta ttgaattcag cacagttaaa aggaaaagga
     1741 ttttttagaa ctgtctatta tattccaacc attacatcaa cggtggctgt agcttccgta
     1801 ttcatgactt ttttcaatcc gactgggtta ttcaatggat ttttgaatgc gctaggaatt
     1861 aacggtgtac agtggttaac aaatcctttt tggatacgaa tatcaatgat catcacgatg
     1921 atttggctta gtgtaggata taacactatt ttatttttag ctggtcttca agggatttct
     1981 aaagagatat acgaaagtgc tgaaatagat ggagcatcga aatttcggca attcttttta
     2041 ttaacgatcc ccatgctaaa gccaatcatt ttaatggcca cagttttagc cactatcaat
     2101 ggcttgggta atttcaatat cccaaatata ttctttggga cttccaatgg accagaaaat
     2161 tcagctttgg tcgtaggagt caatttatat aaaacaagtt ttgaaatggt tgattttgga
     2221 aaagcctctg ctattgcctg gacgatggtg tttgtatctg ttatattatc ggctattcaa
     2281 tttaaattcg gaggtgataa aaatgactaa acaaaggata caaagcagaa tttttacgta
     2341 cgtcattatt gtgtttggtg ccttaatatt tttgatgcca tatttatata tgattgtagc
     2401 ttcaacgcaa agtaatcaag aaattttaag tggcggaatc aatttcaagt ttggggatat
     2461 gctattaaaa aactggcaag ccatcatggc gagatataat tatcccttga ttatttggaa
     2521 ttcgattgtt attgctgtaa tcagtacagt tttagctaca gccagttcta caatggcggg
     2581 ttatgcatta gcaaagtatc gttttatggg gaataaactg ttgttcaata cagttatgct
     2641 atctaggatg gtgccttttt ttaccacatt gattccttta ttttacatta tgagtcgttt
     2701 aggattatcg aatacttatg caggtgtgat catcccttcg attgccagta caacttcagt
     2761 gtttatgatg cgacaatata gcagtcaatt tcctacagca ctgatggagg ctgccagaat
     2821 cgatggtgcc aatgagtggg tgatttttac gaaaatcgct gtaccgattt tatcacctac
     2881 gattattact agtggtttac ttatctttat gggaagttgg aatcaatatc tatttccatt
     2941 agtcatgctt tcagataaag ataaatatac ggttccctta attatcaaaa atatgacggt
     3001 ttctagtaca ggtgagccaa ttaattatgg cgcaattatg ctaattttag caacttcagt
     3061 gatcccaatg gtcttgattt atgtgtgggc gcagtctaaa tttaaggaga atgacattgg
     3121 agctgcaaat aagtaaaaaa aataacgaat gttaaccatc tagttcgttc taggagcaac
     3181 tccacattca taaaaattaa aagaatggcg ggatatccat gaatgaacag acgaaggaat
     3241 ggtcaagaaa aattttgaat gtagcttctg aaaagataga tgcggaatta gacagaatgg
     3301 ggaataaaat tccatatatt tcttacaacg gaaaatatac agacgtaata aaggacagag
     3361 gaatcgactg gtggaccaat ggtttttgga gtggtatttt atggcagctc tttcacaaga
     3421 caaaacgaga aaagttcaaa gaggcagcaa tacaacaaga acaattatta gaatctgccc
     3481 tgtaccattt ttcagacgtt catcatgatg taggtttttt attcttacca tcggctattg
     3541 cacattatca acagtctcaa aacaaacata gtcgacaact agggttacat gcagctacgg
     3601 tacttgctgg acgctataat atttcaggga actatttagt agcatggaat gaccataaac
     3661 ctggttggat gattattgat agtatgatga atttaccatt actgtattgg gcttcgaggg
     3721 aaactggtga tccgaggttc tctaaaattg ctcggcagca tgcagataca acagctaagt
     3781 atctagtgag gaaagatgga tcaaccgggc atatcgcaga atttgatgca gaatcaggga
     3841 aatatattcg gttacttggc gggcaaggat acaatgagaa ttcttgttgg agcagaggaa
     3901 atgcatgggc tttatatgga ttcgcgttaa gttatcgtta cacaaaaaat ccgtactatc
     3961 tagctatagc agaaaagata gcagtaaatt ttattcagaa aatcgaaaaa actggttata
     4021 ttcctctcat tgattttgct gtaaaagatt cgaatattga taccagtgct ggagtatgcg
     4081 ctgcatgcgg tcttctggaa ttagcaggat atgtttcctc tgtacgatca aaaatgtatc
     4141 atgaagctgc tatcaatata ctccaaaaga cagaaaagca ctatgcaaat tggaatatgg
     4201 atgaagatgg gatcattgga ggcggagcag aagcatacca tagagaactg acttataatg
     4261 tgccactgat ctattctgat tattttttta ttgaaggctt acttcgatta gaacagtcgg
     4321 aattagctat ttggtagtaa aaagaggaga aaatacaatg aaacatccag taccactatt
     4381 aaggaccaga caggattact ttgacttatt taaaaagata aatcaaccac tccagccgtt
     4441 ttatcgagca agtggcgcac aaattgatta tatacatcaa ggggtaggat atggaaataa
     4501 aattgcagga atggaaggat tctctcgggt tttatgggga gcggggcccg caattgacca
     4561 attagatgaa cgatggttga aattaattct atcaggaata aaagctggaa cagatccttc
     4621 ccatcccgat tattggggag aaatacatga aagagaccag cgtatggtag aaatgcccgc
     4681 aatgttatta gcgttatatc atcatgaaca attattatgg aaaaaattag atacaggaga
     4741 aaaaagccag attattcatt ggttctcgca aatttttgaa tatgaatgtg cagatggtaa
     4801 ttggcaattt tttaagataa ttgtcggaac aattatccaa aaatttggtt atccagttaa
     4861 agaatcagca ctgaaagaag catttgataa tatagaaaat tgttatcttc aaaatggctg
     4921 gtatcgagac agttcaagag gtcgccaaga ttattataat ccttttgcat ttcattatta
     4981 tggtttgatt tatagcgtac tagaaccaga aaagcccatt agccagatat ataagaaccg
     5041 agctattgat ttttctagag actatattca tttttttgcg gaagatggtg caaatgtgcc
     5101 ttttggtcga agcatgattt atcgttttgc agtttcaagc ttttggtcag ctatgttgtg
     5161 ggccgatctt ttgcctttta aattaggtga aatcaagggg ttagtcaatc ggaatataag
     5221 atggtggatg caaaaagaaa tatttgatga aaaaggtatc ctgaatattg gttataccta
     5281 ccctcaattg acattgactg aaccctataa tagtacgtta tctccattat ggctgaataa
     5341 gattttttta ttgttggcgc tacctgaaga ccatgagtat tggacggcta aagaaatgcc
     5401 tatgcctatt cgtgaaggaa ctaaacttct ttcagaagcc aatttgctca tacaacatga
     5461 taatggacat acattttttt tgaatgcggg acaattaggg ccaaatttcc atacattgac
     5521 caatgaaaaa tatttaaaat ttgcttattc tagtcaattt ggtttttcga taccgcgagc
     5581 gaatcaattg aaagcagaaa tggcaatgga ttcaatgcta gggattcagc gagcggacac
     5641 atatatcacg accagtttta agaatgagag catcaaaacc tatggacagt tcatcgttcg
     5701 aaataatgta cgagacattg tatgtaagaa agaggtactt gcctctacat gggaacctac
     5761 tttacaggca aaaatcagaa cttggcttat tccttttgaa ggctggcaga ttcgcgtaca
     5821 caaagtagaa ttagatacag aatatgttct atatgagaca gggtttgcta ttgaatgttc
     5881 tcctgagaaa gaaggaataa tcatagaaga agacaaggaa aactataggg tgagtgaagc
     5941 tggcttttca ggtattatct gtttaagtga cgatactatt aaacgaaaat ccacagctat
     6001 tatgggtttg cccaatacaa acttgatgac atgggaaaac acttttattc ctggattaga
     6061 gggtacattt ggaaaaggaa cccattggct cggaacagga gtggttgctc atcaagatcg
     6121 ggattatagt ttagaagtat ggaacaatcg tcccaaaatt gattttgatg ggacaaccgg
     6181 gctaactatc caaaataaaa acaataaaaa aagactaaaa ttatgctaaa ggctggtggg
     6241 gaaatgcttg ataaagtgat gtttgaagag ataatacatc cagaaaaatg gataaatgaa
     6301 tgttataaag aattaatgga aaaagaatgg aataatgaga tcgagcgttg tcgcagacac
     6361 acggagaggt taatcaaacg tatcagtaac agtcagtatc aattcccaag cgagtgtcct
     6421 ataaaaggac gttatccatt aagagacaat attggttgga cagaaggatt ttggacagga
     6481 attaattggt tgttatttga gaagacgggt gatcctttat ttcaacaaac agctgaaaaa
     6541 gcagatatag attttatcca caggattgac tgtcaaatta tcactaatca tcacgatctt
     6601 ggttttttgt attctttatc aaccgtagca gattataaaa taacgggaaa tcgaaaaatg
     6661 aaggaagctt caattgatgc tgcagaaaca ttacttcagc gtttttggga gaaggcagga
     6721 gttatacaag cttggggaga tcctagtgat ccttcacaac aaggtagaat gatcatggat
     6781 tgttgcatga atcttccatt attgtattgg gcgagtgaag aaactggaaa tcaaaaatat
     6841 aaaaaagctg ctcaggctca tattcaaaat gcagcagatt atttagtaag agaagatggt
     6901 tcaacttatc atacatttta tatggatgtc aaagatggaa aaccaagata tggaagtacg
     6961 catcaaggtt atagtgataa ttcctgttgg gctagaggcc aagcatgggg gatttacggt
     7021 tttgcattaa gttatcgtta tttgaaagaa caaaaatatt ttgatttaag caaaatatta
     7081 gccaattatt ttatgaatcg gttgccagaa gataccattt gttattggga tttgatcttt
     7141 gatgattcta gcagagaaga aaaagataca tctagtatgg tgatcatggt ttgtggatta
     7201 ttagaattaa tagaatcagg tctattagac aaaacagaaa agaacttata ccagtctgct
     7261 gtttggaaaa ttatgcgaca attgaaagaa agatatgcta caacggaaga agaagatggc
     7321 tttttaacac agtctgttta taataagccc aaaaataatg gggtaaatac accttgtatt
     7381 tggggtgatt actattattt agaagcttta gtgagaatga ctagtaattg gtcttcttat
     7441 tggtgaaaaa ggtaaatatg gaatttttgt ttgataaaat aaaaaaagaa aaaccaaaac
     7501 agcatgaatg gtttctgttt tggttttttc gttaattttc atattcttct aattccaagc
     7561 ttagttcttc ccattccgtc aattttgttt cttgatcttg tctagcagct tctaaatctt
     7621 ggttcagctc attgagtttt acgtgatctt ccaggttttc tggcgcgacc atttcttgtt
     7681 ccaactgatc gatccattca tcgatccgcg ccatttcttc ttcaacctga gtaatttttc
     7741 gttgaaggct acggataagc ttttgctgtt ctttgctttg gtaataatca gatttgttcg
     7801 tgggagcagg agcagattct ggctcatctt gtgccaacaa ttcggcgatt tcttcttctt
     7861 cctgcttttt ctctaaataa taatcatagt ctcctagata gactttgctg ccattttcag
     7921 agagttcgac gacttttgtc gcgatccgat tgatgaaata tcggtcatgg gaaacaaaaa
     7981 gcagtgtgcc gtcataatcg atcaatgcat tttctaatac ttctttgttg tcaatatcta
     8041 aatggtttgt tggttcatcc aaaatcaaaa aattttcttt gttcatcgct aacttggcta
     8101 aagcaacccg tgctttttca ccgccactta gcagagggat tggtttttct acatcttcgc
     8161 cagaaaataa gaaagcacct aaagcacttc ggatctcttt ttctggagtg gtaggatgct
     8221 catcccacaa ttctgctagg atcgtcttgt ttgtatgcag atcagcttga ccttgatcgt
     8281 aatagccgat ggtgacattt gtccctagcg tttcttttcc acggataaaa gggatctggt
     8341 gaatgattga tttcagcaac gtcgatttcc cgataccatt cggacctact agagcaatcg
     8401 cttcttgacg acggatatct agattgattg gttcagataa ggtgacatta tcatatccga
     8461 tagcggcatc ttcgatttgc aagactacat ttccagaagg cttttctata ctgaatagaa
     8521 agtttgcttg tttttcatcc ccttgaggac gatccagccg gtccattttt tctaatgtct
     8581 ttctgcggct ttgcgctcgt tttgtggtag aggcgcgaac aaggttccga gcaacgaagt
     8641 cttctaattt atggatttct gtttgctgct tttcataagc cttccattca cttgctaact
     8701 gttctgcttt caaatctaga tatttcgaat agttaccttt gtaatggcgc atttttcttc
     8761 tgcttaattc atagacttca ttgactactt tatctaaaaa ataacggtca tgggaaacaa
     8821 tcaataatgc accagtatag ctttgcagat aattctcaag ccaggaaagt gtctcgatat
     8881 ctaaatggtt cgttggttca tctagaatca aaatgtcagg gtgttgcaaa agcatgcgag
     8941 ctaaagctaa acgtgtgcgc tgtccgcctg ataaagtatc gattttctga tcataaaatg
     9001 aagaatcgaa tttaaaccca tgcaatacgg aacgaatttc attttcataa ccatagccgt
     9061 ttcgatctga aaaatcgtgt tgcagccggt cgtattcttt taaataagaa ctataagcag
     9121 gtgtgtctgg cgtaccttcg ctaatgaaaa tctctagctc gcgcatccgc tcttccattt
     9181 gtctaacttg ttcaaaagct ttcagcatct cgttccaaac tgtttcattg gaatcaagtc
     9241 ctgtgtcctg agcgaggtat ccgagagtcg ctgttttatt tttagcgatc tttccttcat
     9301 caggtgcttc aattccagca atgatcttta ataaagtcga ttttccagcg ccatttctgc
     9361 caactaaagc gacacgagcg cctgtactga tttctaaatg tatgttttca aataagactt
     9421 ctgcaccaaa atggcgagca atctgattgg cttgtaataa tatcatggaa tcctcttttc
     9481 tactttgtga attctcctca aataaaaaac tatttttcaa ttagtagtct accataaatt
     9541 ccattatact gcgagatttg cagaaatgat aaataaatga ataaagaata aaatttcaca
     9601 atatctgagc aatggaattg attttttcct ataaatactg ctatgatgga gaaatcaaag
     9661 atgtaattag acaatacgtg gaggtcattt cgtgaaagat catattattc caaaggcgac
     9721 agctaaaaga ctgcctttgt attatcgcta tttaaggatt ttaaataatg ctggaaagaa
     9781 aaaagtgtct tcaactgaat taagtgaagc cgttcaagta gatagtgcaa cgatccgtcg
     9841 agatttttct tattttggcg aattaggaaa acgtgggtat ggttatgatg tagaagatct
     9901 tatgcacttt tttgcaaaga cattaaacga cgatcaattg acaaatgttg cgctgatcgg
     9961 tgtagggaac ttgggaagtg ctttactaaa atacaaattc catcaaagta atagcatacg
    10021 tatcagttgt gcttttgatg tgaatgagga aatcgttggg cgcatcgtag acggtatccc
    10081 cgtttatcca atggaagata tgtcagaaca aatcaagatc caacaaatcg aagtagcaat
    10141 tttaacgata cccgctgaaa gagcacaaga agtagtcaat cagttaacgg aagcaggaat
    10201 caaaggaatc ttgaacttta ccgctgctcg attgaatgct ccgtcagatg tattgatcca
    10261 aaatgttgat ttgacgaatg aattgcaaac cttgatctat ttcttgcatt ctgacgcaaa
    10321 tgcgcattcc gaaaatacag cagaataaaa ataaaaagcg attgaacaaa tgaaggcaaa
    10381 actttcactt gttcaatcgc ttttttctgt ttggacaacg attattttgt tcggaagatt
    10441 tcttcataag aaagggaatc atcttctgct gtcagttcat ctagtagata acggattttt
    10501 ttaaagtcga tgaaccgtgc ttgccgaaag acttcttttc cttgatgaaa aatcaggaca
    10561 actggcgcag tcagcacttc aaatttacta gctacttctg gtgtttcaaa tgcatccaaa
    10621 tgaagtgccg gaatatcata atgtgtgaga agttcctcta gtcgtggacg aacagcgtga
    10681 caaacactgc aattaggcat cgaaagataa atgacggcaa cggatgactg ttgcagtatg
    10741 tcttgaactt cagtcaagtt agtggtttgt ttcattttta ttctcccttt tatgatagaa
    10801 gtgatatgta tgagacctta atttaatggg aataacagaa aaagtcaatt gaagtgcttt
    10861 ggtaaaaaat aagagttgtg taaaaattta cagagcaact tgtttttttt gaataaaagc
    10921 gtatatttgc aaagcgaatg aactagaaat aaaaaggagg aaactcatgt tttcttttat
    10981 tccaaattcg gttttgcaga tgagtacgat tttcttatcg atcgttattg aagcattacc
    11041 tttcgtatta ttaggctgtc ttatctcagg cgccttacaa gtatttttga ccccagaacg
    11101 tgtgaagaga tggcttcctg aaaatcgtat cttctctatt tttactggtt cgatcttagg
    11161 atttttcttt ccttcatgtg aatgcggcat cgtaccgatc gttcatcaat ttgttaaaaa
    11221 aggcgtacca gttcacacag cgtttgcttt tatgctgact gctccgatca tcaatccaat
    11281 cgtgattttt tccactttta tcgcatttgg caattcttgg cagatggcaa gctggcgggt
    11341 gataggcagc ttttttgttg cccttgtgat tggtgtctgg cttgcctatt ttcaaaaaga
    11401 cgctgtattg aaagaaaaaa tcacgtctaa agtagcagaa aatcaccatc atcatgtcca
    11461 tgaagaagaa caaagtgaga agaatgaacc aaaaaatcag ctgatccatc aaactgggca
    11521 tgtgttgaca cattcaatcg acgaattttt tgatacaggt cgttatttga tcattggcgg
    11581 attaatcgca gctgcgatgc agacttattt accaactaga attatgctaa cgctcggctc
    11641 aacaaagatt ttagccatta tcatcatgct gttattggcc tttacgatgt ctctttgctc
    11701 agaagcagat gcgtttatag gatcttcttt gttaagtctg ttcggcacag cacctgtagc
    11761 tgcctttttg gtctttggcc caatggtcga tatcaaaaat ctactgatga tgaaacgata
    11821 ttttacagga cgctttattt tatcgctgat cggactgatt gcccttagcg ttattggatt
    11881 cacattattt atttaggagg gaaaatatgc tgcggttttt gattttatct gggtatttcg
    11941 aattgatgat gtacttgcaa gtttctggca aactcgatca atatatcaat gtccattatc
    12001 gctacttagc tattctttca atgattttat ctgctctttt ggctttagtt cagctgtatt
    12061 tgtgggtcaa aaacggaaaa gcagaagaaa aggcacacga gcacgatcat gaccatgggt
    12121 tgtcaaaacc ttatcaacgt ggaatcgctt atgtactgct agctttgcca gtaatcgtgg
    12181 ggacgttgtt tccaacagtt agtctcgaca ctacgatcgt agaagccaaa ggatttaatt
    12241 tcccagtaag taaagaatca gtaggggatc cggagatgca gacacaatac ttaaagccgg
    12301 ataccagtat ttatttcaac aaatcagact acttagacca aatgaatgag ttgaaagaaa
    12361 agtatgggga taagcaaact atcaagatca cagatgaaaa ctatttagaa gttatggagc
    12421 taatttataa ttaccctagc gaattcattg gcaaaaaaat cagctatgaa ggatttgttt
    12481 accaaacacc gaacgatagc aatacagata tcttcttgtt ccgattcggc attatccatt
    12541 gtgtggcaga ttcaggagtc tttggtttgt tgacgcatat gccggaagga gtaacggtta
    12601 aaaatgatga atggtataaa atagaaggaa ccatccaatc agactattat caaccattca
    12661 agcgtgaaat cccatctgta gttgttacaa acgctgagaa agtctctgct ccgaaaaatc
    12721 agtatgtgta tcgttctttt taaaagaaca aaataaataa acatagctca gacagctgac
    12781 acgcaaggac gtcggttgtc tggctttttt tgcatgatgt attcatttgt ttgatattta
    12841 ttttagaagc agcatcctga aagtatttga tatcggtttc aaaggtgttg gaaaagattg
    12901 tgaagagaat tacaatttat tcctaacatg tatgttgaaa agttgtatga aatgattttg
    12961 atagtaaaat atcgagtgga ggtacttttt atggtaacgc gtatatttat tttggatgat
    13021 agtttggttt ttgaaaaaat gctagtagaa attttggaag aatccagaat tttattaagt
    13081 ccttggaaat ttgagatcgt tcagggattg aacgttatac aatttgttga attcattaaa
    13141 aataatgaaa tctattcctc agatattttt ttcctagaca tcgatttgaa cgcctattat
    13201 agcggtatcg aactaggcga aatgcttaga gaaaagaatg aaaagtgtaa aattgtttat
    13261 ctaacgtctc atccgaataa agcaataact gttattaatc gaaagattac gcctagtggt
    13321 tatttgataa aagaattcac ttatgaaaaa aataaaaaaa gcatccaaag ttacttattg
    13381 aaagaaaaag aaaactatcc tgttcaacat gctacgaaaa aaaggtggac aactgtccgt
    13441 tatggtaata gcgatcacta tatccattac gatgaaatcc tttatctaat gagcatgcca
    13501 ggttataaaa ataaaatagg cctagtattg aaagatgaag agattctcgt aaacggcaca
    13561 attaaaaaat ataaaaaatc actcgaatat gagtatttat gtaaagattt aaaagcttat
    13621 atcattaata ctgaaaacat tacatctatt agcagagtaa acgaaatagt aacatttgca
    13681 aatggagaac aactagagat gggaaagaga agtattgata aactaaagaa ttttatgcaa
    13741 gaaaagatag aaaatgaatc attttaaaga aaacaaagac tcatatctta cctgttagaa
    13801 gagtgttttc ctgtagaaga gaacaaccca agtagcagcc tagtcgtttc tatccttttt
    13861 tgttatgcac tctttttgac atttactcca ccaataacga agggtacaat aaaaagtaat
    13921 aacaaacaaa aggagtacag aaatgattga attcatcaat gtaagtaaaa gttacgggaa
    13981 aaaacaagca ttaaaagact tgtctctctc gataagacaa ggggaaattt ttggattttt
    14041 aggacataat ggtgccggaa aatcaactac gattaaaagt ttagttagta ttattgaacc
    14101 atcatcggga acgattttag tagatggaat gaaattgacg gaaaatcgat tgagtatcaa
    14161 acaaaaaatc ggttatgtgc ctgattcccc agatattttt ttacaattga cggctggaga
    14221 atattgggac ttgatttcag ctgcctatga attagacaca caaaaaaaag aaaagcgttt
    14281 agcagaattg acagcgcttt ttgatatgta tagtcatcaa aatgaaacaa tcgcaagttt
    14341 ttcacatgga atgcgtcaaa aaacgatttt gatcggcacg ttgctgccag atccagatat
    14401 ttgggtattg gatgaacctt tgcaaggact tgatcctcaa gcagcatttg atttgaaaga
    14461 aatgatgaag gctcatgccg caaaaggaaa aacagtcatt ttttctacac atgtattgga
    14521 taccgcacaa cagttatgcg ataaactggc tattttgaag aaaggtgaat tgatttatca
    14581 gggggcagtt agtgaattat tgaatggctc acctgatgaa acgctagaaa agatttattt
    14641 gaaaatggct ggaaggcaag cgacaggaga tggaccgtat gtataagcga caattattag
    14701 aattaattcg agttaatctt ttatatgtta atccacagct cacgaataaa gcaagagaga
    14761 aaggaaaaaa gggaaagcga ttaactatct atcttttgtc tcaaaatctt ttttcagggc
    14821 tgattttttt actgatttac ggatttgcta tgtttgctat gaactttgtt aaattgccag
    14881 gtttctttac ttattacgtg gcactttttg gtatgttagg tttaactcaa agtatatcag
    14941 tcatttataa tatctttttt gaaggaaatg acctgcaagt atttttgcct ctgccttttg
    15001 gacaagaaca ggttttttca gcaaagattt tggtagttgc tttgaccatc attccttttg
    15061 tttttccgct tctagtcatg tttattttaa ccggatggcg agctgatcta gggattttgc
    15121 ttactatcat tatctcaatc cttttgtttt ctctattttt gattattctt ttttgtctgg
    15181 ctagtttgat tgtttttggt ttggctcgaa cagcagtgtt taaaaaacat agaaaaacag
    15241 tttctagtat cttgcttggc agctcgatgc tgatcgcatt tgctggaatc atgtggatga
    15301 atcatcaaac ggggactatt gaaaatgagt taacagatcg gcatccgatt tcgatattat
    15361 tgccattcta tgatgctgcc agtcagccac tttctcaaca aggtttattg ggttggggaa
    15421 tactagctat cacggctctg ggatttttcc ttgctgtaaa acagctgata gtaccaaagc
    15481 ttacagagca actgactgat tcaaacagtg agttcaaaat aaaaagaacc cataaaaaga
    15541 accaaaatgt gcatcagctt ttatttagtt acaatgctca attagtaaaa gaacctaatt
    15601 tgattatgca agttctttct tcttccttgt taactccaat catttttatt cttgcttttg
    15661 ccctaggagg cgaaataaaa ttaactgatt tagataataa gttaatcggt gttgttttcc
    15721 tggtagggat cgcattggct ttcttaacag ttaatcagac ttcttttatc agcaatctta
    15781 tttctttgga tcaagaaaat tttctgttta tccgttcttt gccaatctct atgaaccaat
    15841 atttaaaaga aaagttcaga ttcggatgga tattgcagag ttttttaact ggtgggatcg
    15901 cattattaag cggactgtct tttcagctgc ctttgttttt tatccctagc ttgctatttg
    15961 gttctttatt cggctgctac ttattgtcac tacgttattt cgctagagat tatcggttgc
    16021 tgctgctgaa ctggaccaat attaaccaat tatttaacag aggttcagga agtttgggtt
    16081 tagtcgcaac tatgatgggt tcattgatca tcagtattat tcttatcgtc ttatacggaa
    16141 tgttggcttt gttttatcca ttttggttag tgaatattcc tgtgatcggt ttagttctaa
    16201 tactttccgc tctatggatc gtttattatc aacgaacgtt ttggaaaaaa tttgaataaa
    16261 gaagaggggc cgtgacaaaa atcatgtcgc agcccctttt ttacagatag agggtgttca
    16321 aaagtcagtt tctcagtatt aagtcaattt tatgtaaaaa tgtgagaagc tatttttaca
    16381 aaactttttt taatactcat aattacttct tattgctcct gcggttactt gtcgcaatgt
    16441 ttgtgagata ttttgtcatc gctcaaggct gaacgtctct ttcacaacct tttctttgtt
    16501 acacttcgtt tatttaaaaa gccgtttttt tcgtttcatc atgattgcgt cacgctttac
    16561 tttgaaatta cggatgttat ggttaaagac ttctgataat acaagaccta aagcgattgc
    16621 tccagcaatc ataattgctt ggaccgcata atgagccgct tctcgatagg attcagtaac
    16681 gagatttcga accgcttgat aagctaaacc accagggact agaggaacca tcccgggaat
    16741 attgaaaatc gttacgggca ttttcaaacg tttagagaaa agatcgctga tgaatgctac
    16801 tcctaaagaa ccaagcaatg aaccaaaagc agcagatccg cctatttgaa cacagatcca
    16861 gtagatcatc cagccaaaag caccagaaag accacaacaa atcaatgaac gcctaggtac
    16921 atttgtaata acggcaaatg ccgcagtcgc taaaaaacta aaagaaaact gaataaatat
    16981 cgtccccata cttgtgttcc tttcctaata aaaaagctgg aaaataaaag caatcgcaaa
    17041 accgatcatc gtagcagtca tcatcgcttc tgttccgcga gaaactcctg agacataatg
    17101 tccagctagc aaatcacgga tagcgttcgt aatttgaact ccaggaacta aaggcatcac
    17161 acatccgata atgattagat cctgattgct tcctaagcca aaacgtacgc tgtagatagc
    17221 ggctatgcca atcaataaag aagataaaaa ttccgataaa aatttgattc taaaaaattt
    17281 caaactatat agataagctg cgtatcccaa tcctccaatg actaaagtca caggtaaatc
    17341 ggtcatcttt ccgccaaata acaccataat tgttccactg atcactgccg cgctaacgat
    17401 ttgcaaccag acaggaaaga aagaacggtc tcggtcgatt gcttgcagtt cgcagtaaag
    17461 ttcttctaat gttagctctg aagcaacata tttacgtgaa agatcattta cttttactac
    17521 tttttctaaa ttaatcgaac ggttggtaat ttgttccatt cggatggtcg aggtacgatc
    17581 aagaccaata aagagaccgg tttgagtgac atagctgacc aaacggaaat ttccagaagc
    17641 taaagcaatt cgactcatcg tatcttctac ccgatacatc tccgcattgc tttccatcat
    17701 gattttccca gcgagtaaac aagtgtccag caacagatct aattcttttc ccgtcatagc
    17761 atcttctcca ataattgatt gattagtttt attttactac aaaacatacc agaagcgtaa
    17821 agaataaaaa aacttgagga aggaataaaa gaggcgtata ctgtttttgt attgcgggtg
    17881 tagtttagtg gtaaaatccc agcttcccaa gctgatgtcg cgggttcgat tcccgtcacc
    17941 cgcttaaaat aggttgtgaa aaagctgctc tgcaacaagt aataagaaca gccaagcaaa
    18001 aacaacttct tatgttttta acttggatgg acttattacc gcagatgcaa gcttttgcgc
    18061 accgtttacc aagcggttgt gaaatcagtg tattgtcttg agtatgaaga aaatttttga
    18121 ataataataa ataccttaca gatagagtgg tgactgatta gagacgagga atccgtctct
    18181 tttttgtatt tttaagccat cttaaaacag gttttatttt ttgctgtttc tatttctttc
    18241 tagtatttat ttatagggga acatatgtta agatttaggg agctgttagt aaaggagtaa
    18301 agaaatgagc aaaaaaacaa ccaaggcgat cgtggggatc gtcgctgctg tgattgcagg
    18361 ggcattcggt gtttccacca cacaaaaaga ttcagatatc tttcagcaga ttacaactat
    18421 ttttgattcc acaccacaag cttctcaagt agcggatgtg ccagaatatg atggaaaaca
    18481 tcaagaaata gaaatcaatg ggaatcagcc ggaatttaca aaagaggatc ttagcctttc
    18541 caaaggaaca tgggaaagct attcaaatat tgataaattg aacagagtag gacaagccaa
    18601 tgctatgctt ggaaaagatt tgttccctac tgaaaagaga gagcctttgt atattgaccc
    18661 gacaggttgg aaacaaaaga aattaagtga cgggcaatgg ttatataatc gcagccattt
    18721 aattggtttc cagctgactg gtcaaaataa caacattaaa aacttgatga cagggacacg
    18781 ttcgttgaat gctccataca tgcttgctca cgaaaatgat attgcggcat atatcaaaga
    18841 aacaaatcat catgtacgct accgtgtcac tcctcatttt gaggggaatg aactggtagc
    18901 caggggagtg caattggaag cagaaagcat tgaagacaaa aaaattgaat tcaatgtctt
    18961 tatctacaat gttcaagatg gctacacgat caattatgag acaggacaag caacaaaaac
    19021 aaaaaattag tagcagctat tcgaattttt tatgaatatt atgaaaaaat tgtgtaaaaa
    19081 catacattca aagaaatata gcgtatactt tatgaaaaga gtacttttga taaagctaca
    19141 gaaaaaagga aaaaaacata caaatagtgt ttcttaattt attcggaagt ttggaatatt
    19201 tgtttgtagt tttccctttc ttcgagtaaa ataaggttgt agaaggtaag ctggaattac
    19261 cttgcgggaa agaggtgtag aatcatgggt tttacagatg aaaccgtacg ttttggtttt
    19321 gacgatagtc gcaaaaaaga agtgagcgaa acattagcga ttgtttatcg ggctttggaa
    19381 gaaaaaggct acaatccaat caatcagatt gtcggctact tgctttctgg agacccggcc
    19441 tacattcctc gttatcgtga tgctcgaaat ttgattcgcc gtcacgaacg cgatgagatc
    19501 atggaagtaa ttgtcaagga ctacctctca aatcatggag tgaatttatg agagtaatgg
    19561 gcttggatgt cggttctaag acagtaggtg ttgctatcag tgaccccttt ggctggacag
    19621 ctcaaggagt agagatcatt cggatcaatg aagcagaaga agagtttggc tttgatcggt
    19681 tgaaggaatt agtagcacaa tatgaagtag accggtttgt agttggatta cctaaaaata
    19741 tgaacaattc catcggacca agggcagaag cttccatgtc atacggagaa aagatcaaag
    19801 aactattctc cttgccagtt gattatcaag atgaacgatt aaccactgtt caggcagaac
    19861 gtatgctagt agagcaggca aatacttcaa gagccaaacg aaaaaaagtc atcgataaat
    19921 tagcagctgt gatgatctta caaaattatt tagacggctc aggtaagcaa ttattttaat
    19981 aataaaagaa aagaggtaat cttatgacag aacataacca ttcccacgac catgaaggac
    20041 acgaacacat cacattggtg gatgaccaag gaaatgaaac attatatgaa atcctactaa
    20101 cagttgacgg acaagaagaa tttggacgta actatgttct tttatatcct gctggtgttt
    20161 cagaagatga agatgtagaa ttacaagctt atgcatacat cgaaaacgaa gatggaacag
    20221 aaggcgagtt agaacaaatc gaaacagacg cagaatggga catgattgaa gaagtattca
    20281 atacattcat ggcggaagaa gaagaataga ctataaagct aggagaaacc ttgatatgat
    20341 agggtttctc ctttttttat tttttactgt atgccatatt gtatgccatc tagtcaatat
    20401 ttaagtgttg tactaatttt tcaacagtct tttctttcgt ttcttttgcc aagtgaccgt
    20461 aacaatcgat cgtctgacta atttttgtat gccgtaatct agtttgaact tctaccatag
    20521 tagctccagc gtccaagagc attgaagcat gagtgtgtct tgctacatgc acaccgagaa
    20581 atggaaccat agctttttca caaatagtaa tcaatcttgc ataaatagcg tggtgtgtga
    20641 ttagttcttt atagatacca caaaaaatca tagtcggatc agttacacca agtgtcagca
    20701 tatatttccg ctgattaatc tgccattttt ttaatataga aattgttctt tcatctagtg
    20761 gaagaacccc agcactacaa tcagttttag gatcactgat tttatagcca tttttggatt
    20821 ggcttagtgt tttattgatg gtgaccgtat tttcttgaaa atcaatgtct gaccaattca
    20881 atgcaagggc ttcactgatc ctaaccccgc tataaaagag aaagcgtagt aacgttttat
    20941 caaagtcgtt tcgataagtt tcaggtttgc tgtttaggta atcaaacaag gtgatcagtt
    21001 gttctttact gtaaactttg accttttctt tatgcttagt ggtggttctt ttaggcatta
    21061 atacgttgtc tagtggatta gtctcaataa tcccaagatt gatagcgtag ctaaaaatac
    21121 gcttagaagt tgaatgaagt accttgtatt ggtcagtagt ttcagcccag ctatttacaa
    21181 tttgttgggc tttttttacc gtgacttcat tcacacgaag atttccatat tttggaagaa
    21241 tccactttct aaacttgatt tcagttttca tgaaggtggc ttctcggaca gtggttttat
    21301 atgtttctag ccacaagtca taaacttctt taaacgtttc gttgttcgct tttttaatac
    21361 cgtttttatc gtagtccact tgcaagcgtg acaaggcaag ctgggcttct ttttttgttt
    21421 tgtaacctcg tctacgaacc ttatcaggtt tgccagtcat tttgttcatt ccaagatagg
    21481 cttcatagag ccaaaattta ccttttttag tttcatattg cttgaatgtt gccataatag
    21541 ttttccctcc atagtgtagc aagccgtggg ggcggttgga ggaggcagtt tattattatt
    21601 ttgaatagtg tttatttagc tcagtaacaa gagctgtgaa ctcttttaga agatcatttt
    21661 cataaaattc ttgatactct tttttctcat ctgtattttt ttctgaatca ttaattaatg
    21721 agtcatatcg taaaagcgta tttagaagag ctgcgagata agcaattggg gtactcccgt
    21781 caggaaattc aattcgagat cctgttttaa gatattttat tgcgtttgtt aaataaatct
    21841 tctgtgagac agtcaggtta tcttcttcga tgttattgat agcattagaa agattagaat
    21901 caattataga gttatttttt tcaatagttt ttttcaattg cttttcaagc acatttcgct
    21961 taatttcggg catcatatgc atatctctta atgtataaga tccgtttaat agataggtag
    22021 tggatacatc taataccttt gaaagttgtt taagcctggt gttattggga agattatagc
    22081 cattttccca attactaacg gcacctttac tggccggtgg ggataattta cctccaagtt
    22141 gttccattga gagtcccttt tcaattctag ccagtctaat tctttgtcct gtctccactg
    22201 cttcggggta tttttttcta gccacatttt caccaccatt catatatttt ctctactata
    22261 tcacgattta aatatgtgtg aaacataaaa gttcaaaaaa gtatgttaaa attcttgcaa
    22321 atgaaaaggt atgatggtat attgagttta taagaggtat agaaatgtat tcaagagcct
    22381 cttgcatttt tttaaaatct ttgtatagaa atgtatgcta acgagggggg ataaatatgg
    22441 aattaaaaat tgacaaactc acgcttgatg atccagcttt taaaattatt gacgatcgag
    22501 ttgttcaaca agtagagcaa actcaaggaa cgttactttc attattagaa aaaaagtacc
    22561 agtttccaaa atatatgaac aaggcacaag ctgctaagta tatgaacgtt tcatacaata
    22621 ctatgatgca gaaatatgtt cctaacggtt taaagttgat tatagttgat ggagtcgtaa
    22681 gaatttcaca agatgaatgt gatcgcttta tggaagaata ccaaaaataa acgcaagccg
    22741 tgggggcaaa gggattaata ggaggaaata ataatggaat cgacacaaaa taaagcaatc
    22801 gaaaaagtat tatcaacaat tcttacagaa gacacagcgg cagaattagc aaacttggaa
    22861 ggcaaagcgc tagaagaaac gtttgaatgt ttgtacgaac aaatggacta tcaaaaattg
    22921 ctaccacaag aaccaacggt aagcggagta ttgcgtggct tgaacgattt agtacaagca
    22981 gagtttaaag aacgactttc tattgaagag tatcaagaaa ttttgtatca gcaagttgag
    23041 ttgttagcga gcttgctagg gcttgaactg gaggacgttg aatgaaaact gaaatttgga
    23101 acggacacat tataagattt gtcgatatta atgatgaatg gtgggcagtg gcgaaagatg
    23161 ttgcggaagc gttaggtctt aaacaggtta ctagagccat tcactctttg cctaaagatg
    23221 gggttactac tagtaaggtc atcgattcat tgggcagaac acaagatgta aacatcatca
    23281 acgaaaaaaa atatttaccg catggcattc aaaagccgta aaaaagaagc cgaggcattt
    23341 caagactgga ttttcgacat catcaaagaa ctacgccagt ccacaggact tgaaggcttt
    23401 caagtattcc gcatgctaga caaagaacac caaaaagaag caatgactaa attaagccac
    23461 gctattactg aaccaaaacc agtagactac attaaagcga atgtgatagc caataaagct
    23521 gtatcaacta tttatggtca ttccaaaatg gtaaaaaaga aagatatgac tcctgaaatg
    23581 ttggttgatc gtgaacctat tttagatgag acagtagaat taatgactgt taaagaaaaa
    23641 tatgggttgc agttttcagt gagtgagaaa atttataatc gttctgctga attgcagaca
    23701 acttaggagc attaacatga tcgagaacaa agaaaaagcc ttatccataa cagtcttggc
    23761 ggacaggata aaggcaactt aattgggata aaacccgata ttctttgttc cgattatacc
    23821 atattaaaaa atcggaggga accattatga aacgaaactt aattatagga agcattttaa
    23881 tcggcggaat tttcggctta ttaccattaa ttgcaaaaat agctatcttt gcaggattag
    23941 cgttatatct cttcactgct tatgacgagt acgactatca attaagaact ggagggcata
    24001 agtagatgta caaaacaaaa ctattaaacc aactcgatag ccttgaatta gaggaaatta
    24061 atcaagggat cgctgaacta gaaaataata tcgggaaaac ctactttgga aattcattca
    24121 atgaaaaact aacagttttg tatgtactca aaaaacatgc agaacacaaa ataatttgtc
    24181 gagaaatcaa cgagctaaaa aatcagattt taaccgcatg gttaaacatt actgatatgc
    24241 aagaagcaag ggtgaaaaca tttaatacat gggtaaagta tcagaatcaa ttaaaaggtg
    24301 ctgaatttgt tcgggatggg ttgaaatacg aacttgagca gttgaagctc atggaggtgt
    24361 ctgaatgatt tataccagta ttttgaaaaa ggaggagagg tttattggca aataattatc
    24421 aagcagcaac ggaattacaa aagtcgggat ggtcaattta tcccttatct cctggaacta
    24481 aaattccaat aaaaggctct agtggttgga atgatgcaac taacgattct aatcaaatta
    24541 atgaatggtg gacggaagat gcaactagaa atattgggtt aatgcttgaa gatcatcaaa
    24601 tggtggtagt tgacctagat caacattctg aaacacgaaa cggcgttgag aattataaaa
    24661 aacttgcttc aatgtatgat ccatttcctt ctacttatac ggaaatcaca ccaggtaaag
    24721 gaatccattt cttctttaaa attcctgatg gtgtaattat ccaacaagaa acaggtgcat
    24781 ttgctgatat cttcgggcgt gatgataaag gtaaaagttt aagcgggatt gatattatca
    24841 ctaaaggagt tccaatagcg ccaactatga ttatttcaag aagtgttggg agagcatatg
    24901 aacctattga aaacgtatct ctaaataata ttgcaccagc tcccgattgg ctagtgaacc
    24961 tactgctaaa aaaaccgcaa gaaataaaga ctcaataccg acccgtagca aaaacaggga
    25021 cagcaaaaaa attagatatg attgttcaag gcgctggcga aggtagtaga aacgatcatt
    25081 taactaagtt atgcggttgg ttactatggc acggtgtaga taatcaaact ttagcagaat
    25141 tgatttatac agcaaatgcg tacaatgaac caccgctaaa agataaagaa gtgaatcaaa
    25201 ttattcgatc gatgattaaa aaagatttga gggggaaagc tcatggctca taagcataaa
    25261 ttagatagta ttttagactt tcccgaagcc agcgagcgtg aagacaatat cattgaattg
    25321 aaaacctgga tgtcacgatt aagatgcaat aaagacgatc agattaaaag taacagtgtt
    25381 gtgaatgcag aattgatttt aaccaatgat agcaatttag ctggaacaat tgcttataac
    25441 gaatttagcg gatacattca cctattaaaa gattctcctt ggatcaatcg taccgctgga
    25501 gaatgggaag acagttttga ggatgcctta acggcatata ttgaagaaaa ctacaatgta
    25561 gtttttgatg acaacaaaat acacaaagca gtagtaaatg tggctagaaa gaatgttttc
    25621 aacccagtaa aagaacgtat cgaaaaagtt aaatgggatc aacagcctag actggaaaca
    25681 ctgtttattg accttttagg ggtagacgac aacctttaca cacgagaagt aacaaaacgt
    25741 tggatagtcg gatcagtcgc acgtatctat aaaccaggaa taaagtttga gatcgttcca
    25801 gttttggatg gaccgcaagg gattggtaag agtaccgttc cagcattact ttacacggat
    25861 gattttttca ccgattcatt agattcgcta ggtgaaaaga aagatgatta tatgcagctt
    25921 caaggcaatg taattattga acttggagaa ttatcttcaa tgaacaaaac taaaattgaa
    25981 cagataaaaa acttcatcag tgccaagatc gataaaatta gacctcctta tggtcggaat
    26041 gtcatagctt gggcaaggca atgtgtattt attggaacgt cgaatgatgg gcagtatttg
    26101 aaagatgata caggaaaccg ccgtttttat cctttgcctt gtaagaataa gccaaagatg
    26161 aatccgttta aaacaaagga tgaatatttt cttcaagtgt tagctgaagc aaaggtgctt
    26221 tttgataaag gacaacgaat ctattttagt cccgatgaag ataaagaagt cttagaaatt
    26281 gcaaaagact atcaagagga tgccaagatg gagaatccaa ttaaagaagc aatcactaaa
    26341 tatttggaaa tggaaatccc gtataaatgg gaagaagctc cagcgtgggc gagacgttct
    26401 tattatcaga actatcctga cagtaaatgt aatgagaaaa atttacagca tttctataat
    26461 ggagctacta tgcaacaaga gttttattta gttgatagcg tactcaccgc tgatattttg
    26521 gaagctgttt ttgataaaca agcaaaggat ttattgaatg gtcgatcaga tgcagaaact
    26581 aaaaagattg ccttgattat tagcagtatt cctggttggg aacggaagca actgccaaaa
    26641 agaaataaac gacgaggatt ttacaataag tcaaatgcct atgaaaataa aaatcgagct
    26701 ggaaagtaca aaaagtaacg ggtagtaacg ggtcagtaac acctagcccg ttactagttt
    26761 acccttactg ccccaaggga tacagcaatt tggtaacggg gtaacacctt atttccttaa
    26821 aacttttgaa atagttagct aatatgctgg ggaataatgt tactggattt tacccgttac
    26881 ctgttaccaa aacgctagaa cccttgtccc cgttgggatt gagtggtaac gggtacctgt
    26941 tacgtaggtg ttaccacgtg ttacgaatca aggaggaaag taaatgctca atattatttc
    27001 aacaaacaaa gcaccgaact ttcaatatac agatgaaatg gatcggtttc taatgaacac
    27061 gctggcattt agtgtgggat tggtaacaga agattattcc acatttgatc cagaagtatt
    27121 aaagataatg gaagaagaac ctgattggct gcaggagtca gtagcgttgt gtcaatcgct
    27181 agtagtggga tcattggtag atagtggcaa ctatgatgat acaggtgagc taatggatga
    27241 atttaattgt ctgcttaacc tatatgatcg agcgagacaa cgagagctta catcgaatga
    27301 ggataatttg tttttaaaca tccatgataa gttcttggct ttgctactaa cagatgatga
    27361 attgataact aatttattgg aggtagaata attatgcata tggaaagtat tagatacttc
    27421 gatggtgaga aacacattga gtattcaaac actcaagaag atgtagactt tattagtttt
    27481 gaggaagata aaatcactaa agttaatttt aaagacggaa cgtgcttgaa gatagtttcg
    27541 ccttatattg aatacaaatc aaaatgggaa caagatgatt ctgacgaacg agatcgcttt
    27601 ggttggtaat ctatggcagt taaaaaacaa tgtaatcatg ctggatgtaa aacattgatt
    27661 gattataggc agaagtattg tgggaagcat aaagcaaagc aaacggcaat taagcgtgaa
    27721 gaaagaaagc aaagcgaagg taaatatttt caattctatc aaagtagaac atggcgaaag
    27781 gcttcatact tgtatcggtt aaatcatcca gtatgtgagg attgcctaga agaaggcttg
    27841 ataaggaaag ctgatgttgt agatcacaaa attgaactaa aagatgattg gaatagacgg
    27901 ttagatgaaa ctaattttcg ttctttatgc catgcacacc ataattccaa gacagccaat
    27961 gaaagacaaa gacgagaaaa gagtaccctt tgagttaagg gggcatagtg tgaaacttac
    28021 tgacaatcga tgcctactca ttttggtaca aataaccgtt gtgaaaaggc ataagggtaa
    28081 ttacaaataa cgattacact tgtaatcaca agtgaaaaag tatataatga aagtaggaga
    28141 tttattgaaa aatacaattg ttattactga taaaacaggg aaacagcgca ccattgagtt
    28201 acctcatttt ggtcaagtga ccattcaaat gcagaacgga aaaatcattt atatagataa
    28261 attagataaa gaaaaattct gatcgaaaaa cgaaggaatg tgagcttaat tgcttacgtt
    28321 cctttttctt ttgtctgaaa ggaggacaac atgggacacc ctaaaatatt acacgataca
    28381 aaaggcaata tatcaagtga agaaaaagca atccgtgtgg acgctagaga agaattgttc
    28441 aagcagcaac cattaataaa tatcacgccc cctgactgga tggcagcgag cgctagaagt
    28501 gaatggaatc gtatcgtacc aacattaaaa aaagattatc cattgagtga agcagattat
    28561 ggttcattgg tagcctattg tttagccttt gctcgaatga aaacagccga agccgagata
    28621 agaaaatcag gtacgtttat cacatgcgaa aacggagtaa agaaagccaa tccagcggtt
    28681 cgggttcaat cacaagctat gagtgatttg aaaaaacaag ccacctcact aggtatgacc
    28741 ttagaatcac gatcaaaact agctttgaac aaggttaaaa atgatgaacc cgaagacccg
    28801 ttcaaagagt tgatgaattc atgaatgatt acattgagaa agtcctatca ggtaagctga
    28861 ttgcacccaa gaaaattatc caagcgtgtg agcgtcatat aagcgatttg gagcgttcta
    28921 agtcagatag cttcccttat gtgttcgatg aagaacaagc caccaaagcg attaaattca
    28981 ttgagttgct accatctacg gacggtaaag caatcaagat gttaggattt caaaaattca
    29041 ttctaggcag tctttatggc tggcgtacta aagaagggaa ttacaggcga ttcaatcgag
    29101 cgtttaccag tatgagccgt aagaatggga aaacgtatat cgcaagtggc atggctgcca
    29161 atgcgttgat tatggaacaa gaaccagcag aagcaaggca agtattgttt gtaagtaacg
    29221 ctttgaaaca agctaaattg ggctatgata tgctgtctaa ttcacttaga aacgtggtca
    29281 agtctagtaa gtttttaaga ccacaactga aaattatgaa ctctaagatt cagcacttgc
    29341 cctctaattc gttcgctatg gcactggcta gtgaaaccag cacgctagat gggtttgcac
    29401 caacaaccgc aattcttgac gagtggcacg aagcaaaaac tcgtaaaacg tacaacgtca
    29461 ttaagtcagg aatgacccaa caaaagaacg gcttattgtg tgttattagt accgctgggc
    29521 ttgatttaaa cgttcctatg tacgaggaat acttattgtt agatcgtgtg ctaaaaggcg
    29581 aagaacaagc cgacaggtac tttatagcga tatgggaatt ggacgatccc gaagaaattc
    29641 acgatcaaga gaaatggatc aaagccaatc cgatttttga aagtgaagaa atcaaaaaag
    29701 taatgattcc aaccattcaa gatgatgtga accttgcttt gaaacaaaat aaccttaatt
    29761 ctgtattggt gaaaaacttc aatttatgga gacaagcgag tgaggacagc tatatgattg
    29821 ctgaagactg gcaggcaacc gaagtagaac cacaagatat tacaggcaag cccgtttata
    29881 tcggagtgga tttatctaaa acagatgatt taactagcgt ttcatggatc gtaccgctag
    29941 ataacggcga actttattgt gattcacata gctttgtagc caccaaatat gggcttcaag
    30001 acaaagaaaa gcgcgacggt ttgccttatc gagaactaga aaaagctggt gagtgttcca
    30061 ttactcaatt agaaagtgga atcgtggact atgaccaagt atttcagttt attcaagatt
    30121 tgattcaaga aaatgatttg gaatgtatgg ggatttgtta tgacccgtat aacgctaatt
    30181 cgcttatcag taaagctgaa aaagccaact acccaatgtt agaagtaaga caaggaacga
    30241 ttactctaaa cgttccgacc cgaactttta gagaacaagt ttatgaaggc aacgttattc
    30301 acaataaaaa tacgattctc acccatgcag tgaacaacgc tattttaaaa acggataaca
    30361 acggcattca gattaataaa tcaaagaaca gtaacaaaat tgatccaata gctgcattaa
    30421 tcaatgccta tgtgtttgca atggattact tcaccacaac ggaaggagcg aaagcagaca
    30481 atgaatttta tacaagtgaa gaattttctt tctaattaca ttcataccgt tcttttactt
    30541 cttggattgg tgtgtgtgtt ggttgctatc accttactaa caaatgtcta ctatggcttg
    30601 ttagcgctgg gcatagtgct tattggggta gcggtcatgc taaatacaga acagaaggga
    30661 ggttaaaaga tggcattttt taaagcgaga caaaatacaa cgggagatcc tttcctacaa
    30721 catgtggtat caatccaatc ggatgattac accaccagct ttacaagcgt tcgtgcatta
    30781 agaaatagtg atgtgtttgc agccgttcga attattgcta gtgatattgc ttcaagtccg
    30841 attcaattgg tcaaaaacaa tatgccacaa gctgataatg aactggtgaa gttactaaac
    30901 gagaaaccta attcagaaat ggatggttgg catttaaagt ttgctttggc ggtcaatatg
    30961 ttgctaaacg gtaatagctt tgcagagatt aaacgcaacg gtgaaaaggt agaagaaatt
    31021 cacttattac ctaactcaag tgtaacggtt actcaattag ataatggtac gttgtcttat
    31081 cagattggtg ataaaaaaag acgtgtgaag tctagcgata ttttgcactt caaatatttc
    31141 acccaagatg gtttaacagg attaccacca ctttatgcgt tacgtgatga actgaaaatt
    31201 caacaagctg gcaaccgcac attgcacaat ttctttactc gtggtgtcag tgggtcaggt
    31261 attttgaagg ttcataagtc agatttagac ggatcagcaa agaacgcaat tcgtgaaaag
    31321 tttgaagaag ccaacggttc aagtagtgga gataatgctc ttagaacgat cattcttgat
    31381 gaaacaatgg actacaaaac attagaaata aacaccgatg ttttgaagct tatcaattcc
    31441 aatgattgga acacaaaaca aattgccaag gcgtttggtg taccgattga gcgtttaggc
    31501 gttgaaaatg aacattctag caccgttcaa agtaatctcc agtatatcca aagtacgctg
    31561 attcattact ttaatgtgtt tgtgagtgaa ttagatacga aacttgaaac caatatccgc
    31621 tttaattccg atcagttact agagactgat cctgaaacaa aagtaaaaaa tgtattggat
    31681 caggtcaaag ggtcacttct cacgattaat gaggggcggt cgaaaatggg gctacccccc
    31741 atggatggtg gcgatcgttt actagcaagt ttgaacttta cgtatttaga tacgttggag
    31801 aaatatcaat taaaagaaca ggaaggagtt acaccagttg aataatgaag aagaaaagga
    31861 aaaacggctg acagaagaag ctgagctaac agccgattct cccaaagtag agaaagaaaa
    31921 tgaaaaacaa ccaacagacg gcaaaactat ttcaggctat gcgttgaaat tcgggcaacc
    31981 gtcaaaagat ttaggcggct ttgtagaagt gattacaccc gaagcattaa aagaggtgga
    32041 tttatcaaat gtgttcttat tatatggaca cgattattca aagccactag ctagtgtcaa
    32101 agcaggtacg ttgaaattga atgtcgatga tacaggatta cattttgaag cagagctaac
    32161 cgacacgacc tattcaaatg atgtgtatga gaatatctca aaaggggttg ttgattctat
    32221 gtcctttggg ttcgtgcttg gattagattc attcgacaaa aaagaagatg gcacaattga
    32281 acgatcaata gataaaatca aagcacttaa tgaaattagt gtcgtgaccg ttcccgctta
    32341 tgattcatca aatgtccaag taaataagcg ttcttacgaa tcgtttatga gtaacaacca
    32401 agcaaaacaa acaaacaata gcttagaatc cacttctaaa gcacaaaagg agagtaataa
    32461 catggaaaaa actttaatcg ataacgaaaa aacagaattg cgtgggtatg aagaatatat
    32521 ccgttcacaa ggcgaagtgc gtgatggagt cactactgta aatgcagcgg cagttgttcc
    32581 cgaagaagta atcggtgaag tctttgattt gaaacgttca aattataact tagctcaata
    32641 tgcaacagta aaaacagtat caaatggaca aggtaaatat ccagtagcaa ctaaccaaca
    32701 agcagtgtta gcaacaaaag ctgaacttgc tgaaattggt gatattgacg ctgaaatgtt
    32761 tacttcagtt gattataaag tagaaactcg tgctggtaag attgccttat caaatgaggt
    32821 tgtggaagat tcagcagtga atattgtaca agaggtcaaa gatcagttag cgaaattggt
    32881 agaaaacact gacaataagc atatcatgga tttattaaaa acattcacta agaaaacggc
    32941 tgctacgttg gacgatttga aacaactata caatgtggca ttagacccag cattaaataa
    33001 aatggtaatt ctaaaccaaa gcggatatta ccacctagat acattgaaag attcagatgg
    33061 acgttacatt ttacaacccg atgtgacagc acctagtggt aaatcattat tcggtatgcc
    33121 agtagtattg attgcagata cattgtttgc caatcctaaa gctggaacat tccctatgat
    33181 tatgggggat attgcacaat ctatctttgt tgctcgtaga aatcaagtaa cgactcaatg
    33241 ggaaaaattc gattactact cacaaggact tgcagtgatc gttcgcaacg attataagaa
    33301 aattgatcaa aatgcttcag tgtatattga gtttacgcca gttgtaacac caaaagtata
    33361 gaaaaatatt gggcggcggt ttatcccact gccttttttt cattaggaga gataaaatgg
    33421 taattttgga tagcataaaa aaaagtatgc ggatcgatca cactattgat gatgacttta
    33481 ttcaacaatt gattgataca gcaggcgaat atataaaaag tgctattgat agtagcgcaa
    33541 cggataaaga tatggataat tatcagcaat ttgatttggc ggtgtcatta cttactcaac
    33601 attggtattt gaaccgtcaa gaagccagca gcgaacggat accaataacg gtacaagcat
    33661 tagtacaaca aatgagaggt gcttattatg ccaatcatta agaatgtaaa tgaattgact
    33721 gagagagtca agtttaaaaa gacaaaacgg gtaaaagatg aagatgggca gatggtggat
    33781 agtgaagaaa ctgtatttga atgttgggct aatgtgcgtt cacaaatgct aaaagacgtg
    33841 cttgctagtg taggtaccat tcttgaagga acgttgacgt ttattattcg atacgatcaa
    33901 gattatgaac taacaaacga tatgaaagta gcttggaaaa acaagagtta taaaattatt
    33961 tcaatcaatg aaggaacggc gtttaaagat tatacaacga tcatagctaa acagatttct
    34021 tagaatgatt acacttgtaa acgttgtggg tatttagtat aattaaggta gtaaatgaag
    34081 ggttagctgc ctgaattttg caagacttag ctagtcgaaa tctattgttg gactgaaaat
    34141 tgtaggtgtg gttgcaaaca tgctggacta agaaagatga tagatgatga acgttcaatt
    34201 ttaatacagg catgctagga ctgattggat ggttttggca agctgattgt gtagaaatca
    34261 aagaacacgt ctgttgtgga cgtgtttttt ttgttataat tcaaaagagg tgatttaatg
    34321 gacaaagcag agtttacaaa tttttattgg aagtattatc tacatttaga aaatgagttt
    34381 attaatacca cagattttgt gatgctcgat gagaataatt tcaaaacatt ctctattgaa
    34441 tatcaaaaac tactactagc gattggttcg gagtgtgaga ttattttcaa agaactttgc
    34501 ggatttgata gcagtagcaa taaaagcata actgatttta aagctgttat taaatctagc
    34561 gaattaatgt gtttggataa tggtgttagg gttatgaatt ctttaacatt gacttcacta
    34621 aaaccttttg gagatgggtg gcctgaagat acaccagatt ggtggaaaat atataacaga
    34681 gtaaaacatg gtcgttcttc aaattataag gaagcaaatt tggaaaatgt gttgtatgct
    34741 ttagcaagtc tatatttatt agaggaatat ttgcataaga aagtaatact agaaggtgaa
    34801 gtagacttta taagtccaga gtcaaatcta tttacgttat cttgggaaag aaaacattcg
    34861 tcgatgcaaa aagtgacgtt tgaaaaagtg gattcaaatt atgtagctcc actcattgat
    34921 tttaaagaac aagaagaaat ttaaaaaccc ccacggctta ttgaagtttt tgtatgccat
    34981 attgtatgcc atttggttta aatcgcttga aaaatagtga tagataactc taattcattt
    35041 agtcataaac gctgttttga taacctttat agttctttga tatgggtttc tcattcatgg
    35101 cggaagaaga agaatagaac aaaagaatga attaataaga tataaaaaaa gggattgtga
    35161 caaacgtctt gtcacaatcc ctttttccaa ataaacgatg ttcaatcagc tgcccctcgg
    35221 cattcgctcg aaaaacggcg aaatatgaag aactattttg ccatttgttt ctctaataat
    35281 caggtcaaaa cgttgacttc acagtctctt ccttaaacgg tgttctcctg ccaactcctc
    35341 ggtcacaagt cacaatattc aacaaacatg agaagctgtt ttttgaaatt gttccttgtg
    35401 actcggagct gaacggcagg ctcacgacct ctttcttaaa cggtgttcaa aagcttgcat
    35461 ctgcggtaat aagttcaacc aaacgaaaat tatgaggagc tatttttgtt tggctgttct
    35521 tattacttga tgcaggacag ctttttcaca acctcttttc gtttagcact aaatttcctt
    35581 aaaaagtaga aaagctttcg aagcctcgtt tgtttctttt actgtataat aaaagaaaag
    35641 agtaacaatc gctaaaagat ataaacaaga gaaaggaaat acaagatgat tggtctgatt
    35701 tattttgtgg tgatcgttct ggcgaacaca gttggcgcta tttccgggat gggtggaggc
    35761 gtgttgatca aaccgattct tgatcttatc ggcgcccatt ctgttgcagg gatttctttt
    35821 tactcaacag ttgcggtatt cacgatgtcc attgtttcga ctgtgcgaca agtatcaagc
    35881 gggaaatcac tgaattggca gattgtcggt tgggtatcta gtggagctgt tgttggagga
    35941 gtagtcggca acattgtatt cgaagttttt ttacagttat ttgaaaacga gaagcatgtg
    36001 cagctgatcc aaattttttt gacggtattg accttggttt ttgcgttctt ctacaccaag
    36061 catcatcagc caaaatttca tttgacgtca tggacttggt accttatatg tggggggggt
    36121 tctgggcttt ttagctagtt ttttaggtat tggtggtgga ccagtcaatg tttctttgtt
    36181 gatgctgatg tttgcattgc caataaaaga agcaacacta tattctctat ctacgatttt
    36241 cttttctcaa ctggcaaaat tggtaacgat tgctttaaca agtagcttta tgcgctttga
    36301 tttatctatg ttgttttatg ttatccccgc tgctgttatt ggaggtttgt ggggagcaag
    36361 atttagcagg gttttgtctc caaaaaaagt gacctttatt tttcaagtaa ttgtcctcgt
    36421 ggttcttttg gtcaattgtt acaatgcctt tgtcatctta caaacattag gagaatctag
    36481 actaacgcaa gtcaaaatca ctgaaacata ttgttctaaa tgaaaaaaag agtatactat
    36541 gataaaaaaa ttcattttaa aaaatcagaa attggctggt gatttggatg ttacgggaaa
    36601 tgcgtttaga agatgcagaa gcactacgtg aaatcaatgc ggatcaatta gggtatgata
    36661 taccattaac cgttacggtg agacaaatga agaaactgct gaaggagcca gagaaaaatt
    36721 ttttcttggt ttatgaagat cctacttcta aaaaagcagc tggctatgta caagcggagt
    36781 cctatgaatc gatttttagc gatccaatgt tcaatattat ggcacttgct gtgtctaaat
    36841 atgctgaaaa aagaggcatc ggaaaggctt tgatgactgg cgtagaagaa gaagcgaaaa
    36901 aacgaggaat aacgacaata aggcttaatt ctgcagaata cagagtagaa gcacaccaat
    36961 tttatgaaca catcggttat cattccgata aaatgcaaaa aagatttttg aaaatcctgt
    37021 aaatctttat caaaaaaata cccgatcacc aagccgatac ttgatgatca ggtatttttg
    37081 tttttttgac taagaaagta attgcatcaa gaaaccagag atgatcaaaa cgatggcacc
    37141 accaagtcga ttgcccattt gagcaaaagc aataagttcc atccgattag ctgcagataa
    37201 gacggctaca tttcctgttc cgcccatcga gttgttgcat aagccagcag taatagttga
    37261 ttcaattgga tataaaccaa acaaacgacc gacaaatcct gaaacaactg aaatcgcgat
    37321 aacacttgtc agacataaga caacaaattg ccatgtcaaa gcagaagcta atacattcag
    37381 attcagcaac gcaataccga taccggcaag aacagctgct gtcaagtttt tgacaatcaa
    37441 gttattgaac atgatagctg catcttcata atactctggt aataggttgg cgattttaca
    37501 gatgacaacg ataataatca taaatgcgta agcatggact ttaggaacaa aatagttacc
    37561 aatttgtcct aagatgaaga aagtcaatga taccatcaaa ccaacaccta actgtggaat
    37621 ggaaggtttc acagttcgag gttttccatc gccaagatcg cctttttcca ttaatttacc
    37681 attgccgttg aattttggca ttgattgtcc agcacgagca actaatgctg caccgatgat
    37741 agctaagaca tttgtcattg ctgtcgcagg aatcaggcgg gaaatgatag aagaagcatc
    37801 cgtacctaaa acatttgcat aaatcgttga taaaggaacg gctccggctc caacaccacc
    37861 agccatcgca ggtaatgaga tgtataaaac ggaatttgca aaaccatttc cgataagcat
    37921 gcccacgagt cctacggcaa aaaaggagac gaccattgaa ataagtgcaa cgggaataaa
    37981 acgaacagaa gctttcatca atagattacg gttcatacca aggatgctac cagtaatcaa
    38041 ggcagcaata taaaagtccc aaaagcccat attgctgaca aagttttctg tggatttcac
    38101 tacatattca ggtaagatcc caaatgtagc aatcgcagca gaagcgaaga tcgcaaaaac
    38161 ggaaccacca cccaaataac ttttgacgat agggattttt gtcccgataa aatgaaataa
    38221 attaccgaaa ataaccaaaa ctgcgattgg tccaatcata ttcgtcggta atttccctaa
    38281 agccattgca atgatcagga caaggaccat gatcaaataa agtggcaaac ttacgccggt
    38341 gatttttgtg gaccaaaaac cttctttttt cttttcctca actgcagttg cagttgttgt
    38401 tttttgtgcc atttttacag atcctttctt attctgtatc atatgaaaaa agccaaagaa
    38461 aagcgacaaa agagcaggag ttggctggcc gctgttttgc cgcagtcgct tttttctttt
    38521 tagacgtcat acaaagtttc tgtatgttct tttactataa cgatcttaat cttctaaaga
    38581 tttatattca ggaacccatt tagcagattc aactgcttct ttgatatccg tgatttcttc
    38641 acgattcaag ttttgtgcga caactgattt tgcgacagtt tcagcaatcg tttgagaaaa
    38701 ttcagtgatt tttgctactg gaggcaagat agctgctcct ggttttgtta catcaacgat
    38761 tccgcctaat gctcgactag cttgagaaat gatttcagca tttactcgtg tagctgttga
    38821 agcaatcaaa ccaagtccta aacctgggta catcaaagca ttatttgctt gaccgatttg
    38881 gtaagttaca ccattatatt caacatcagc agcagggata cctgttccaa ctaatgcttt
    38941 tccgtccgtc cattctatta aatcttttgc ttttgcttct gccaattttg ttggattaga
    39001 caaaggcatg atgatcggac gtggtgtatg agcagccatt tctttcacga tttcttcagt
    39061 gaatgctcca ggttgggtag aagtaccaat catgattgtc ggatggatcg cttttacaac
    39121 tgcttctaag tttgtcaatt catcagcatt agtgaaatct gagcgtttgc gagcgaatgg
    39181 gatttgacca ggagtcaatc cttctgtatc ttcaaacaat aaaccttgtt tatctacttg
    39241 atagaaatgt tggcgtgctt cttcttcagg tacaccttga cggatcatct catctaaaag
    39301 gatgtttgcg atacccacac cagcagtacc agcaccaaat gtcaaaatta tttgatcttt
    39361 caattgttca ccggatatat tcaatccgcc taatacacct gctaggacaa caattcctgt
    39421 accttggata tcgtcgttga aagtagtgat ttgatcttcg tatttttcta agatcgctgc
    39481 agcatttcct cgtccaaaat cttcccaatg caataacaat tctgggaata attcggtagc
    39541 tgattgaacg aattgatcga tgaattcata gtaagtttcg ccttctacac gagcatgacg
    39601 attgcctaag tataagggat tattcaataa ttcttcatta ttagtaccag cgtcgatcga
    39661 tacaggcaaa acttgtgctg gattaatacc agcagcagca gtatagacca tcaatttgcc
    39721 gatagcgatg tcaacaccgt tcacacccca gtcgcccata cctaagattc cttctgcatc
    39781 agtcacaaca atcaaacgaa tgtctcgtcc atctgccgcg ttcttcaaac ttgctttgat
    39841 gttttctggt tcgtcgattg aaagaaatgc agcatcttgc ggtttcaaga aaatttcgtt
    39901 gtattgttcg attgaatccg caacgacagg atcataaacg acaggcatga attcaactaa
    39961 atgttcgccc atcaatttat aaaacaatgt ccgattcgta ttgaatagat tcattaagaa
    40021 gatacgtttt tccaaatctg tttgtttgct taagtattga ccataagctt gaacagattg
    40081 ttgttccagt gtttggacag tacttggaag cataccagtg attccatatt ttgcccgttc
    40141 ttcttttgta aaagctgttc ctttatttag aaaaggatta tttaataagt ttaaccctgt
    40201 aagcatgaat gattcctcct aagttttaat acaaacgaag tatagaagtc agatgatgat
    40261 atagtcaaaa agagcaggta tgataaattt tatttatatg taatatagcc aaaagaaatc
    40321 aaaatagcag tatttgttct ttttatatcg acaatccttg cttgaatact atgcgtatct
    40381 cttatatgct agagaaagaa aagtagaaga ataagggata gatggaatgg ataaatagga
    40441 tttctgagga aaaaagcaca tgatagcgtt gataggagtc acctatgtca ggtgataaaa
    40501 atacataagt agaaaattaa gaggaggaag agaaaatgaa agaagttcaa ttgattagaa
    40561 aaagcgaact atcagaaggc ggttgcaatg catgtggcgt agtagaagcg acaagctata
    40621 cattaaaatt agattccaat caagcaataa tctctgaact gacagtaggc ggtcttgtgg
    40681 acagtctggc gcttgctgaa ggttttactg gagaagatat ttatgagatg ttttcagaag
    40741 tccgtcagtt aaaaaaagga gaaaaatgca tagaggttca tcatgagagc ccgaatgtga
    40801 ggttcaaacg tggtgataat gagatgatct tcaagaatca tgtgtctgat cacactgaac
    40861 tatatgaaat cgtgaatcaa atactgtctg ggatttttga attaggacct tatgagttca
    40921 aggaagaaaa cggtaatcca aaattaaatg aagaatggca agaaacgatt gaaacacaaa
    40981 gaaacaatcc acacttattt caatagtctt ccacagtatt tcgtaaaaat gttgatgaaa
    41041 aagaaacacc gtgaatcaga ggtcgtgaaa aacctgccct gcatcaagta ataagaacag
    41101 ccaaacaaaa atagctcctc ctatttttcg tttggttggg cttattaccg cagatgcaag
    41161 tttttgaaca ccgtttaagg aagagactgt gaagtcaacg ttctgacctg agtattagag
    41221 agacaaatgg caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca
    41281 gctgattgaa caccgtttta tcgagaggtt gtgaaaaagt cgcttagttt cgagtagtaa
    41341 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgactta ataccgagaa
    41401 aatggctttt gaacaccgtt tattcaaaaa aagggacttt gacactactt ttgtcaaagt
    41461 cccatcggtt ttttatctat ttttattaca atcatttctt ccagagtagt gacatctgga
    41521 atggctgaaa tgtaaacaga ttatttgcct gtatcaatgt cgaagagtaa tataaaaagt
    41581 gtaaacgcta tcatattgtt catcattcaa attttataga gagaaggcat tgagatggaa
    41641 aaaacatatc aagcaggtac aaatgaagga atagtagatt ttatcaatat ggaagattta
    41701 gaaatcgcgg cttcacaagt gattccagct ggtggttatg gttacatcag cagtggtgct
    41761 ggcgacttat ttacttatca agaaaatgaa cgggcattca atcatcgttt gattattcct
    41821 catgtattaa gagatgtaga attacccgat acaactacac attttgacga agaaatgctg
    41881 actgcaccaa tcatcatggc accagttgct gctcatggat tagctcatgt gaaggcggag
    41941 aaagcttctg caaaaggtgt agcagatttt gggacgatct atacagcaag ttcttacgct
    42001 tcttgtactt tagaagagat aagagaagca ggcggggaga aagcaccgca atggtttcaa
    42061 ttttatatga gcaaagataa tggaatcaat ttggatattc ttgaagtggc caagcgtaat
    42121 ggagcaaaag caatcgtatt gacagcggat gcaacagttg gcggaaaccg tgaaacagac
    42181 cgcagaaacg ggtttacctt cccgctgcca atgccgatcg ttcaggctta tcaatcagga
    42241 gtaggccaaa cgatggatgc tgtatataaa tcttccaaac aaaaacttag tccaaaagat
    42301 gtagaattta tcgctgctca ttctgatttg ccagtctacg tgaaaggtgt tcaatcagaa
    42361 gaagatgtct atcgatcatt agaatctggt gctggtggaa tttgggtatc taaccatggt
    42421 ggccgtcaat tagatggggg gcctgctgcg tttgattcgt tgcaatatgt ggcagaagca
    42481 gtagataaac gtgtaccaat cgtgtttgac agcggtgtac gacgtggaca acatgttttc
    42541 aaagcaatcg catcaggtgc cgatcttgtt gctattggca gacccgttat ttatggattg
    42601 tctttaggag gaagtacagg agtccatcaa gtgtttgatt tctttaaaac agaattggaa
    42661 atggtcatgc aattagctgg aacacaaaca gtagaagata tcaaaaaaat aaaattgcgt
    42721 gagaatcgtt ttatttaaga aataaatgac gaaaaaaata ggagtgcggc agatgatgcc
    42781 gtactcctat ttttatagtt caaggatgtc actaatagaa taatttgaaa cgaaaaaata
    42841 aaattgaaat attttttcat acttaataaa aaaggaagaa tattcatgta aacgatttta
    42901 ttgttttttt ctcttttata caaaaagggt cataatttca agtaagcgga tgaattcatg
    42961 aggatttttt gcatactgtc tctactatac ttaggtcact aagaaaaagg agtgatttat
    43021 tatgaaaaag aacatgattg ggacattatt actttcaaca cttcttctag gcggcttggc
    43081 aacaccagct tttgctgagg gtcaagcgac ttcaaaagga gatattacct ttactgaacc
    43141 aacaaatacc gttgagcctc ttaaccctac tgatccttca aaaccagtag agccagcaga
    43201 tccggaaaat ccagcaactg gccagacagg ttctttaaca ttggatgtag ttcctgaatt
    43261 gccatttggt acacacgaga ttgaaagcgg aacaaaaacg tatcaagtag acgcttcaaa
    43321 aaacgataca ccttatctcc aagtatcaga tcgtcgaggt gtaggtgcag atggccaagc
    43381 acaagggtgg aacgtgacag tttcggtttc agattttgta aacggttcac aagttttgca
    43441 aggcgctgaa ttagattttg gaacatccac tgttaaaagt acgtcagata acgaatccac
    43501 tggaccaact agtcaaactg taactgggtt gagtaaagca agtgcagcga caccaatatt
    43561 tacagcagca aaagatcaag gtctgggaac atggctttct gtttatgatc cagcaaatat
    43621 cacactgaaa gtgccgaaag cagcagctgg aacattcacg gctgatttga cttggaacct
    43681 cgtggctggc ccagtagcat agagtatgaa ataaattcaa caaactgcgg tgaaaatact
    43741 ataggtgaag gattttttac cgcagttgtt gttgaaagga tgtggtggac aatgaatcaa
    43801 agtaaacgac tgtttgtttc gtttttttct attttaagtc tatttttcat ttttccgtca
    43861 atcagccaag caaaggattc agcaggagac tttggaatca aaccggtatt tccagagaac
    43921 caaatagata aagcaatcgg ttattttgat ttattagtgg caccggaaca aaatcagaca
    43981 ctagaagtaa ttatcagcaa ttcttcagat gaagaacgaa catttgaggt ctctgtcaat
    44041 ccggcggtaa cgagtgatgg tggaacgatc gattatagtc aaaaaaaccc aacgttagat
    44101 gagacattgc cgtttgatgt ccgtgacgtt ttgctcattg cgaaaaagga aatcaatgta
    44161 tcagcacatg cagaaacaac tatacctata gaagtgaaaa tcccagcaaa aagctttaag
    44221 ggacgtgtat tagcgggtat tcatgtttca cctaaagaag aagctgaaac agaaaatgct
    44281 acagaaggag cacaaatcaa aaatcgtatt gcttataatc tcgcagtcgt tttacaagaa
    44341 agtcaagaga cgatagaacc ggatctgaaa cttttatcag gagatttgga cgaggtcaac
    44401 gcaaagccaa ctgttcagct tcgtttccaa aatcctcagc ctaggattat cagcaatttg
    44461 atatttacga gcaagatctt ttatgaaaat cagctttata ttgaaaatac gtctaatgaa
    44521 tttttagttg caccaaattc gaattttcac ttgaatcttg atttagctgg tgataaagca
    44581 aaagccggcg attaccgcgc agaaatcata gcaaaatcag gcgacagtaa tgaatggcga
    44641 tttacacaaa actttacgat caaaaaagaa aaagcacaaa aagtaaatga aaattccgtt
    44701 tttgcagtac aagaacaatc tttcccatgg ctttatgcag gattggttgc tgcagttcta
    44761 gtgttatggt tgattttgct gtttttgtgg gtccacaaga aaaagaaaaa aggagaaaca
    44821 acatgatgag gtgatttcgt tgaaaacatc gatcttattg ggagggatta ttgcaagttt
    44881 gctgttcttc agctggcaag agccagtcca agcagatacc aaaagttcag gtacgattac
    44941 attttatgat ctaagtccag ttccgcatcc aagtggttca gtcaatggcg catcaaaaca
    45001 gcagacacag ctaccgattt ataaagaatc taatcaacat ggcgtattcc cggagacgaa
    45061 tgaagaaaag atccgttttc tttatttgat cattggtata gaattatggc ttcttgcgtg
    45121 gttgttgtta tggagaagaa aggaaaagga ggatgaacat gttgctacaa aataaatttt
    45181 cattttttcg ctccttcttt tttacttcgc tttttgtttt aagtatattt atcttttcac
    45241 cttcggcaga agctgttgga gcaacgcctc ctgccatttc ttatgatcca tacacgtatg
    45301 aaaatatcgt acctccaaat ggtggacgta gtgcagtcaa tggcgggaag cttgtccaac
    45361 taactggggc taaccaattg cctaaattga ctgctactta tttgaacgat ggttcaaatg
    45421 atccttggaa aggtggcacg gtccgttttc attgggaaag ccatgtgaaa ctaacaaatg
    45481 tacttgattt ggtgaatatc actgcacaag gatttgcatt tgtggcgatc atcaatttgc
    45541 cggaacatgt atcaagcagt gatgtattaa aagcaattga ctggaacaac gcctacttgt
    45601 atattgccgg tgcccccatc aaggttacta gcggtagttt attatcagca ggaagccata
    45661 ccttgcgttt tgggatgggg tcgtgggata caaatactct attggcagat attattgcta
    45721 ttattacgcg aggcgaattc aacgtggacg atatcccgct tcaatttgat gttacagcta
    45781 atgtcgcgaa gatgacagaa aacggagata aatatgatac gagtccaaac aaaattttga
    45841 ccaagggtaa attacaacct tctatgtcta aaaaggccgt tttttctgtt gatttttatg
    45901 attcgaataa tattatccaa gatcgtatgt tcaattcgat tttagctcca gctgcaggta
    45961 gattttatcc taaattaact ggcggaaact tgaatccagg agcatatgca ttttctgcaa
    46021 gtgcagagat aaatacgtgg aatagttaca tctcaacaag tgattcaaca ggaaaatata
    46081 atacatttgc tgataccaca gaatacgtgg atggaaataa ccagaacctt ccaggctcta
    46141 ccaatagtcg gacattgacg atggggctga ctgaaggacg ttttgatcag ttgccattta
    46201 gtcgctttga tcgcgtagtc aactatttta caggaaaaaa cgttacacaa ggagcaatac
    46261 tgactcatac tccagcaagc acaagtggat tgaatcagac agccaatatc gtgtacagtg
    46321 ggaaagatgc gcaaggcgga ttgctttctc ctgttgcttt aaaggtattg gagcagtaca
    46381 agctagacgg aacggtttta ccaacaaact taacagccga tagtcagtgg ggaaagctcc
    46441 tttcttctgc atcgtaccaa gtcacggcca attggtcagc aactgaacta aaaaagggtt
    46501 cgatccatta caggctttat tcgaaaggaa cccaaaaatt agcgacagga tggacagatc
    46561 aacttttcca aacgatccaa tcaaataacg ggggtgtaaa tacagctact tctacactac
    46621 ctgcattggc ttcagggcaa tattattttg attatcgctt ggtggacgat gtacttacta
    46681 acatgtatcc gtcttttgcg tacaaatggc agtctgaaca agctggaatc accactcttc
    46741 ctcaaataac agtggctaat tttccatctg tgagtgcaga tagtcagcta aaaaacctta
    46801 gcagacaacc tgagacagga gatcccctgt ctgctctatc aggagatacg attcaagaaa
    46861 atgtgacatt cacgttaaca aaatctgggg atgcgataac cgacaaaaaa atcacgatct
    46921 cacttcctga aaatactact tatatcaatg gttccttaaa attaaatggt acaactatcg
    46981 cagataccgg cattcaacaa ggagtttctg tcccagcaga tctattgagc aaaataggag
    47041 ataccattca tcttacttac aactatcaat taaatactgt agatacttct gtacaatccg
    47101 tcagcatact gacaaaggca gctgtacttt ccagcaatat tacattagca gatggtgcta
    47161 agcttcctaa cccagttgtc caaacatctg ctaaaacaat tcttgtcccc aaacaagaat
    47221 taacattagt taatgtacca gatgatttta catttggtaa cgatctcccc aaaccgttga
    47281 agacaagtta ttatgaagca aaaggcgatt tttcttttga tgtgcgggat actcggcttc
    47341 caagcacgtc tccttggcaa ctgacaggga cattgacttc tttatttaag aatgatcaag
    47401 gtcaagaatt aagcggaaca aaattatact tcaatcattc tggatcaaaa caattgattc
    47461 agcaaggtca aaacacgttg atttatgaga gtgatggaac agctaaagga gaggttctcg
    47521 ttgactttcc cgataccgat ggtttactct tagaggtaaa tagcagtacg aatgctcaac
    47581 ctggcgcaac gtatcaaggc atggtcacat gggaactgac agctgggcca accagctaag
    47641 tgattagaat ttgtatggat ctgtgataac tgtcacagat cctctttttc aaggatgaga
    47701 aggaaacttc tgctgttaaa atcagaaatg cttggaggag tggacttgga acttttcagt
    47761 ttgcttgaaa aaaatgaaca acggcaaatc aaacttttcc aacttttatt agaggaaaaa
    47821 ggagagattt ccgttaataa cgtacttacc cgcttatcag ttgatcgggc taccttgaaa
    47881 gaagatctgt tttatttaca agctaacttg gaaaatatca aacaattgga aattgttatc
    47941 caagaaggga atattttatt aatcagacat ggaaatatag cagcagtcga tgtttattat
    48001 cattgctact tgcgctattc tacaaaatat cagatacttc tttatctgtt ggaaaatggc
    48061 tcatttgatc ggcagaaact tgtcatggaa cttaatatca gttcggctac tttgtctcgc
    48121 agagtgaagg aattaaatga ttctttaaat gagtttcatc tgaaaataaa aagcgggcgg
    48181 ctgaccggtc cagaaagtca aatttgttat ttttatttcc aattattttg gtttggacgt
    48241 ccttatcttg aaaacaaaaa agaatacagt gatttatcag gaaacacttt tttagatctg
    48301 ctgcaaaaag agtttagttt tactattaca gaagaaggac gattgaaatt tgaaatatgg
    48361 ctttccatta tgaagaagcg aatgcgctgt acgaaaaaat ccaaaccaga ttatagcaat
    48421 cttcctgttg gattgtttca aacgacacaa tttttattgg acctccatac atttttgacc
    48481 cgttacttct ttcaatatgc ctttccttgg aatcaaaatg aaacgttggt tttttatctt
    48541 ttcatgatca gtaactttac attagataca cataactcgc atgtccagga gtttttgata
    48601 agcgaaaaat acaaagacga cgtagtagct caattgaatc gaacgtttaa acaatcgata
    48661 gaagaatatt tctcttttta tgtttttcca gaagagattt cgcaaaagat tgcgttgaca
    48721 gtgatgcaaa atcacgctca ttttgcctat tttagaggat ggatgaatat ttttggccaa
    48781 gaaggattaa aacagcggtt aactgaagta gaagataaac aattcctggt tttatgtgaa
    48841 agattggtca gacaaacatt tgctattgtt gattttactg aagtagacga ggaaaatgcc
    48901 cgctttgaat tatttgctcg ctatgcatct attttgcgga cgctgatcaa gcagttagaa
    48961 ttacgtctgc aaattgctta tgatcttcct tatgaaaggc taatggcgga tatcgtgaaa
    49021 gaaatcatcc aggaacaatt gctgaatacg atcaaatatg atttagtcga atacgagaaa
    49081 gaaaagcagt atgatatcat tttgactagc caattgaaag agtacccgtc acagaagtcc
    49141 gctaaagttt ttgtattcac tagcaacgaa tataattatg atttccccta tttgaaccaa
    49201 tttttaaaag aatgttattt agaaaaattg aatttaagga tgaataaaac atgaaaatcg
    49261 tgatcattgg tgcttcccat gcaggcatca cagctgccct aaatctaaga aaacttcaac
    49321 cgaaagcaga agttttactg atcgatgaag atcataaaga tggactaggc tatgtttcaa
    49381 atggaatcaa cctttattta aaaggaaaga tacgtagttt agctgaagtt gcccataaca
    49441 tgcggactct tcaagaaagt ggggcaaaac tgatcaccga atggagagta actgaacttg
    49501 atccagacaa acatcagctc atcctcgcat ccaaagaagg aaaaaacgag accataacct
    49561 atgataaatt aatcgtagca acaggttcct ctcctgtcac cttgtataaa caaattgaag
    49621 ctgaaaatgt ctacacctat aaaaatctag tgcaatctaa gcaagtactt gccgctttaa
    49681 aagaagcaaa agaagtagtg atcttcggtg ctggttatat tggattggaa ttagcagatg
    49741 ctttgagaaa taaaggatac attattcatc tagttgatta tatgccgaat gttttaacaa
    49801 gatatttcga taaggatatg atcaacagtt ttcaaaatca gctgcaaact aagcaaatca
    49861 atttttatcc aaatgaattc ttgatcgatt ggaaaaaatc agaagagaaa gtagtatccg
    49921 tacaacttct atctcaagca ataaaggcag atatggtcat tttttctgcc caaactcgtc
    49981 ctaatacgac attgttaaaa gaaaaagtgg ctttgtatga ggatgagact gtgatagtca
    50041 atgaatatct gcagacaagt gatccggaca tctatgcgat tggagatatc gtcccagtca
    50101 gttttgacaa aaataaacgg catctctttt tacctttagt tactcgagct gtacatatgg
    50161 caagagcagt agcgctaact ttgagtggtc agccaaccgc ttacgacctc agacaaaaga
    50221 taacagcagc agtcattata gactactttt taggaactgt agggctgaca gaagatgaag
    50281 cgccattctt ggaacaaaat accggttcgt gttcaggaga atttgatcta tttccacaat
    50341 acgatgaaga aaacaaaaca gtaaatgcaa agctgatcta ccatccggat acattagaaa
    50401 taattggcgg acagctgata agccaagaat tcttattatc agatttgaac ctgcttgcgg
    50461 atatcgtgaa acacaaaact acgattccac agctagcagt tgaggatttt ggatttctgt
    50521 ctgaacacac ctctcgtttt cactacctca atgagttagc ttttaaagta ctagctaaga
    50581 gagcaaatag caatagagta gataaaagac tgtgagaaat atcacggtct tttattgtgt
    50641 ggggatataa taaatattaa tcaatcgaga ataaaaaatt catataatga aatagcggaa
    50701 gaattttttg agcataatat tcgccaaaaa tttatcaagt gataaaattg ttgtataaaa
    50761 tgattatttc aggaggagga gttaaatgtt tttagcatta aatgaaatta tgcattcaaa
    50821 gttacgttat gcgttagttg caggggtgat gtttttgatc gcctacctgg tatttttttt
    50881 gactgggtta gcttatggct tagctcaaga taatcgaaca gcagtcgaca agtgggaggc
    50941 agattcgatt gtgctttcga aagatgcaaa ttcaaatcta gggatgtcca tgattactaa
    51001 gaagatagca gaagaagtcg aaggtgggaa agtggcgtat cttgcccaga cacctggagt
    51061 cgtgactagt aaagacagta cagaagaagg aaaaatcaat gttagcttct tcggtatcga
    51121 taaaaatcaa ttcatcatgc ccaatcttgt tgaaggaaaa gcttttgata acgatgatga
    51181 ggcagtaggc gatattagtt tgaaagaaga atatggtcta gccgtaggag atacagttaa
    51241 gttatctggt agtgacaaga cgtttaaact aactggcttt acggatcatg ccaaattcaa
    51301 tgtctcacca gttctctata caaccatcaa tgcttaccaa gagatccgct tcgaaaaaga
    51361 agatacaagt gaaaatgcac ggattaacgc aatagttgtc agaggtaaaa tcagtaattt
    51421 acctgaagat ttagaacaga tcaaaatttc taaattcatc aatgaattac ctggatataa
    51481 tgcgcaagta ttgacttttg gatttatgat cggatttttg atcgttattg cggctattgt
    51541 gatcgggatt ttcatttatg tactgacgat gcaaaagatt aatatctttg gtgtaatgaa
    51601 agcacaaggg atcacaggag gatttattgc gcgttctgta gtggcacaaa cattcatttt
    51661 atcatttgtt ggtattcttc ttggtctatt aggaacagtg ggaacatccc ttgttctacc
    51721 tgatgcggtt ccattccaaa gcaattggct gttttttggc gtcattagct tgttgatgct
    51781 ggtagtagct gtgttaggcg cactattttc tgttcggaca atcgtaaaaa tcgatccgtt
    51841 gaaggcaatt ggataagaag gaggaagagc atgaaagcaa tagaatttaa agaagtagaa
    51901 aaaagtttcc aagatggcga ccaagtagtt aaagcattga aagaaacaac catgagtatc
    51961 gataaagggg aatttgttgc aatcatcggt ccttctggtt caggtaaaag tacttttttg
    52021 actttggcag gcggattgca gacgcctagc agtggctctg tatttatcaa tgatcaagaa
    52081 ttcagtaatc aaaaagaaag tgaacgcgtt aaactacgtt ttaaagaaat tggctttatt
    52141 ttgcaagctt ccaatctggt tccttttctg acagtaaaaa agcagttatt attagtagat
    52201 aagatcaaac atgaacatcg tcaaaaagaa gcaaaagaat tatttgagca gcttggcgtg
    52261 gataagttga tcaacaagta tccagaagaa ctatctggtg gagaacggca aagggtcgca
    52321 atcgctcgtg cattgtacaa tgatccgtcc attattctag cagatgaacc aactgctagt
    52381 ttggattcag aaaaggcacg agaagtagtc gatatcttgg caaaagaatc aaaagacaaa
    52441 aataaagcaa cgatcatggt tactcacgat acacggctga ttgacaaatg cgatagaata
    52501 tttatgatgg aagatggtgt attgaaagaa aaagaataac caaaaagcag gtggaaaaat
    52561 ggtccatcga tacaacaaag atacacagca aaaaattttg aaagaagcga ctgacttgtt
    52621 tatgtccaag ggatatctcg gcacttcaac aagagagatc gcacaaaaag ttgggatcac
    52681 ccaacccaat ctctatcatt atttcggtga taaagaaaaa ctatatacgg cagttttaga
    52741 aaaccactta aaagatgttg gaaaagcttt gagagaaatc gttcaaacaa gcgaaacggg
    52801 atttcaagta accttgacaa aaatggcgca gtttttgatt gagacacatc tggtcgattt
    52861 gtttctgatg cttcacgatc ttgaaagcaa tctttcaaag gaaacaagag atcatctttt
    52921 ctttctatgg aagaaaaatt atcgtgaacc ttttgaagaa atcttttcag caaaccaatc
    52981 tgtgctaaga aatgggattt caaaagaaat cgcagcaagg cattttttct tagtgttagc
    53041 accttacatc actcggtcga aaagacctgt ggaagaaccg ctaaatgtgg cgcaattgat
    53101 cgatttatat ttgaatggtg tattaggaga agcgtaaagc tgttatatag ggagaaaaat
    53161 agttaggtga agaagcaaga tatgacgaag atcgttatat cttgtttttt ttatataggt
    53221 agtttttcat aaatacaaat ttgttattct ctcatgatta tcagggtata tttttattta
    53281 ttatgtcggt gttccaggaa gtgttttaat ttaataacga agaaattaat tgtttgtaga
    53341 atgaaggaaa ctatttattg ggtagatgaa tcaccacgaa tagtcaaaag gagatttcag
    53401 gatatgccaa aaatgaaaaa aatcatttta gcagtaatgt tattttctac ggttatatct
    53461 tttacagccc cttttttgcg tacttaagct gattttaagt tttgaccctt agtctggatc
    53521 tacgaggtga gagtcaagaa tgaaaaaaat aattggagct tttgtatttg caatcattat
    53581 cattattatc tgttttcaaa tagatgtttt tggtcaaaaa aagatagttg gacaaacggt
    53641 cagcagtacg aacagtacgg ttacaaagac aaaggaatca tttgatgata cagaacaaga
    53701 gacattaatc aaaatcggaa aaaatgaccg tacactatat acagatgact cgctaactac
    53761 cccacttgca actataaacg gcggagagct ggcggagttt ctttctgaaa caaaaaatgc
    53821 ttatcagatc aagacaaatg atggctatac tggatattta gccctcgaag atggaaccaa
    53881 agtcacaaag aatatacaaa caaaaccgga agagctatct gatgcagtga ttgtattaga
    53941 tcctgggcat ggcggaaatg atactggggc attaagcaat gatgaacaaa cagaagaaaa
    54001 agatataaca ttaagtacag ccataaaagt aaaaaaggcg ctagaggatg ctggagcaac
    54061 ggtttatttg acccatacta cagatgaatt agtacagctt ggagatatat gcgactacag
    54121 tgaagaaaaa aaggcagatg tctttatcag tttacacgca gactctacgg aatatgcgaa
    54181 tgaagcaact ggaatcacga catattatta ctatggacaa gaggaaacat tagcacagac
    54241 aattgctgac tccttcactg atctgcctct ggattcaaga ggaatctcca ctgggaacta
    54301 tcaagttctt cgagaaaatc ttcaaccttc tattttaatc gagatgggtt atatgaataa
    54361 tgatagtgat ttaaaggaat tagtaacaga cagttatcag cagcagattg ctgcaagttt
    54421 gacacaagca ttgaccactt attttcagta ataaataagc ccagccaaac aaaaatagct
    54481 cctcatattt ttcgtttggc cgggcttatt atcgcagatg caagcttttg aacaccgttt
    54541 aaggaagagg ttgtgagcct gccgtttaac tccgcgtcac aaggagcaat ttcaaaaaac
    54601 agcttctcat gtttgatgac tattgtgact tgtgaccgag gagttggcat gagaacaccg
    54661 tttattcgga acaagggact gtgacatgac ttttgtcaca gttccattat ttttttagca
    54721 gcttcaaagc tttttctgta ttggcaaaat gaagtttggc atatttttca agcataggca
    54781 tgccgccttt ctccaatagt tttgccgcta cttggtcaga tttcgttcca gctcctgatg
    54841 gaagggtgat tccagcttca gctgatgctt tgtgtaactc ttccaaaaag ctggcacgac
    54901 tgatgataga agcagcagca acagctaaat gatattgttc tcctttagta acgaagtaca
    54961 gtttttcttg taattgattt ttttctaaac gcgcatattt tttaaaattt gcttccggcg
    55021 taaactgatc gatcaaaatc gcttctggtt tttctggctc gatttttttt agcagcaaat
    55081 ggatcgcttg attgtgcaaa gcgactttca tccgaacggc attgtatttt ggttggatct
    55141 cattgtattt ttctggagat aagacaagca gtttataggg gatggttttt tttaagatgt
    55201 cagcaagctg gatgatttgg ctatctttta actcttttga atctctaaca ccaagtttac
    55261 gtaattctgc aagctgatca cgtttcacat aagcagcaca tactgttagc ggaccaaaat
    55321 agctgccatt tccgacctca tcactaccta aaacggataa ttgactgata ttttttggca
    55381 aagaagaggc tgtttttgct ggcttggctt gtttatcctg tgcctccccc catttatcag
    55441 cttctattgc tgcattcgta ccttgaaaca tcacttttcc ggatgtataa gcggtaatgg
    55501 tcgtcccgtc tttttttgca gcaaagctag tataaggaat ctgtttattt aacaaataag
    55561 agcgatagta tgctttcatt tgactaagtt ggtcgtttgt tgctttgata atgacgttgc
    55621 tcataaaatt ctcctcgttt ttatacataa aactataccg caactcacga cttttcgttt
    55681 tctaagagat tgcgagacat agcatttttt taaagtttat catataatag ttaagtatgt
    55741 gaaaaatatt tgtggagaac ggtgaaagaa ccaacttcat ggtacactaa ataaaacttt
    55801 catcaaatag gaggaaatga ttcgtatggc gcacgaaaaa acacgctaca aagcagtgat
    55861 tgccaaccaa acatatacta ttatcggacg ggaaaccaag catcatatgg atatcgtaac
    55921 gaagctgatc aatgaacagt tagcagaatt gaaacaatta tcaccacaaa tggataatga
    55981 acaagcagca atcttgatgg ctgtcaatgc gctttcggat caattgaaga aacaagaaag
    56041 aatcttggag ctggaagaag aaacagcaga attaaagaaa aaaatgatca aatttacaga
    56101 gcttgaaaat cgtgtgaaac gcattgaagc aatcgagaac gaagctcggg aagtattgaa
    56161 agaaaatggt caagcagatc ataccattaa aaatcatgtg gaagcacaac agattttaaa
    56221 cgagaaacga aaaggacaaa ttaaacaaaa atcttctaat taagatcctt ttatcaaaat
    56281 aagaaaaatt taaaaaagtt agaagagaaa ggaacgtagc atgctgagtt tgctgatttt
    56341 atttttattg ctaattgcgt ttttttctgg agcacgtcgc gggtttgctt tgcaaatcat
    56401 attcgcgatt ggatatgtat tgtcatttat tgctgcccag catttttaca aaccattagc
    56461 aagtcatttg gaattataca ttccataccc agctgtcacc ccaacatcaa agttagcatt
    56521 ttttgatcag gtatttgctt ttcacctaga tgaagctttt tatgcaggaa ctgcttttct
    56581 gattattttg ctaattggct ggctgcttac ccgatttgtg ggggtttttg tccatggatt
    56641 gacgtatgtg ccgatcttga gacaggtaga ctggattgca ggaggcatcc tgtcactaat
    56701 catggcttac gttactatat ttttgatttt gcagttactt gcctttgttc cattagatgt
    56761 ggtccaaaat caattcggcg agcatagttt cgctcgtttt atcgttgaga gaacgccatt
    56821 tcttaccaac aagatgtatg agctttgggt aacgaatatt ttgaattaag gaaaatagtg
    56881 ctggaaaata tttcgttttt tgtacagagt agtttattta agaaggctgc gtagcgtatg
    56941 tccggcacag ctcttttttc atagtgaaag ataaggtgat aaaatgaata accgaatcct
    57001 tgaaacattg gaatttgaaa aagtcaaaca gatggtcaga caatttgtag tgactgcaca
    57061 aggaaaagaa gaactggcag aattggtacc agtgagtgag aaacaaacga tcacaaactg
    57121 gcttcaagaa actgaggatg gattgaaagt gcaacgtctg cgaggcggga tccctatacc
    57181 taaacttgaa aatatccgtc cccatatgaa acggatcgag atcggagcag acttgaatgg
    57241 agtggagctt gcacaagttt ccagagtatt gtctacgaca agcgaattga atcgtttcat
    57301 tgatgatctt tctgacagtg aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt
    57361 agtcacgatc cctgttttga gccgccgttt aaaggaagca atcgatgaag acggacgcgt
    57421 gactgatgac gcttctcctg agttaaagag cataagacag aacattcggc gaagtgaaca
    57481 agctgttcgt gaacagttag atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga
    57541 tgcgatcatc accatgcgaa atgaccgcta cgttatccca gtcaaacaag aataccgcgg
    57601 tgtatttggc ggtgtagtcc acgatcaaag tgcttcagga cagactttat ttattgaacc
    57661 aaaacaagtg gtggatttga ataatcgttt gcgtcaatac caaatcgctg aacgaaatga
    57721 gattcaacgt attttaagtg aactttctgc tgaacttgtt ccccatcgtc aagaaatcat
    57781 ccacaatgct tatgtgattg gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa
    57841 agaagtaaaa gccatcgtgc cgggaatcag tgaagacaat catgtcgtct tgaaacaagc
    57901 aagacatcca ctgatcgatc aggaaaaagt agtttctaat gacatcacaa tcggaaaaga
    57961 ctatcaagcc atcgtcatta caggacctaa cacaggtggg aaaacgatca cgttgaagac
    58021 tttaggtctg ttacaactga tggggcaagc aggattaccg attcctgcag gtgaagaaag
    58081 tcaaatcggt atttttgagg aagtatttgc ggatatagga gacgaacagt cgattgagca
    58141 aagtctatcg accttttctt ctcatatgac gaacactgta gatattttat caaaagtcaa
    58201 tgaaaaaagt ctggtattat ttgacgagct cggtgcagga acagatccgc aagaaggtgc
    58261 tgctctagca atcgcaatct tagatgatct tggtaaaaag tctgcttatg tgatggcaac
    58321 aacgcattat cctgaattga aagtttatgg atacaatcgg gcaaatacga ttaatgccag
    58381 catggagttc gatgtggata cactaagtcc aacgtatcgt ttgctgattg gcgttcctgg
    58441 acgaagcaat gcgtttgaaa tctctagccg attaggtctt gatacagaag ttattgatga
    58501 agcgaagcag ttgatgaatg atgagagcca agatttaaac gaaatgatca cagatttaga
    58561 gaatcgccgg aaaatggcag agaccgaata tctggaaatg cgtcattttg tttctgaggc
    58621 acaagaactt cacgatgatt tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa
    58681 agaaatggaa aaagcaaaga aaaaagcaaa cgaagtcgtg tcagaggcgg aagaaaaagc
    58741 agaaaaaatc attgctgata ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa
    58801 agagcatcag ttgatcgatg caaagaccca attagcaaat ctacatcaag aagaaacttt
    58861 gaagaaaaat aaagtattga agaaggcgaa agaacaaaaa acgttgaaac cgggagacga
    58921 agtgttggtt acgacttacg gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg
    58981 gcaagtagaa atcggtatat taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc
    59041 tcctcaaaaa gagccgacac aacgagttat ccatgctgta cgatctgaat ctagtagtca
    59101 cgtgccgaat caattggacc ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga
    59161 tcaatatttg gattctgcca ttctagctgg ttatcctcaa gtgactattg tccacggaaa
    59221 aggaacaggc gcacttcgca agggaattac tgattatctt aaaaaccatc ggagcgtcaa
    59281 aagctttgag tttgcaccgg caaaccaagg cggaaatggt gcgacaattg ttaaatttaa
    59341 gtaaagctta ctaaaggtaa gcagatggtt cgctatccaa gtatagaaat gctatactaa
    59401 tcatagattt taaaaatgga ggtctttgat atgtcacaag taattacaga taagacattt
    59461 aatgaagaaa cagataaagg acttgtattg attgattttt gggcaacttg gtgtggtcca
    59521 tgtcgtatgc aagcaccgat tttggatcag ttggaacaag aatatgatga agaagaattc
    59581 agaattgcta aaatggatgt ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg
    59641 agcattccaa cattaatgtt gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt
    59701 cattcaaaag aacaactacg tcaaatgatc gatcaatatt tataaaaact tacagcaaaa
    59761 ataagctgag ggtgggactg aatccagttc cgtcctcggt ttttttgcgg ttgagttaag
    59821 gttattcaaa ttaatatttt ttgagtataa ttacgttgtc aggctggatt gtttttttga
    59881 cagacatgct ataataactt taattgaata tgaaggggag taccagatga acgaacgaat
    59941 caaaaacaaa ttagctttac ttcctgatca gcccggctgt tacttgatga aagataaaaa
    60001 tggaacgatc atttatgtcg gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt
    60061 tactggcagc cataatacga aaacggaacg gcttgttagt gaaatcgaag actttgaata
    60121 catcgttaca gaatcgaata tcgaagcatt gctcttagag atcaatctga taaagaaaaa
    60181 tgatcctaaa tacaacatta tgttaaagga tgataaaacc tatcctttcc ttaagataac
    60241 gaatgaaaaa tatccccgtt tggtgattac tcgtaaagta ctaaaagaca aagcactgta
    60301 ttttggacca tatcccgatg tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt
    60361 atttcctctg agaaaatgca aacctagtca gacgaaggag ccttgtcttt actatcattt
    60421 gggacaatgt ctgtgtcctt attatttcga cgtagatccg aaagtctata cgaaaatcgt
    60481 tgatgaagtg aaaaagtttt taaacggagg acacgaaaaa atcgagtcag agatccatca
    60541 gaaaatggaa aaagctgctg aaaacatgga gtttgaaaaa gctgcagaat accgagacca
    60601 gatccgtgcc attgagacaa tcatgactcg ccaaaaaatg acaaatactg atttactgga
    60661 tcgagatgta tttggttatg cgattgacaa aggatggatg tgtgtccaag tgtttttcgt
    60721 aagacaagga aagctaattg aacgagatgt ttcaatgttt cctttctata acgaagcaga
    60781 agaagatttc ttgacttacc tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa
    60841 agaagtcttg attcctgata cgatcgacaa gcccagtgta gaagctttgt tagctacgaa
    60901 agtgctgcaa ccaaaacgag gagaaaagaa aaaattagtt gaattggcga ataagaatgc
    60961 ctcagttgct ttgaatgaac ggtttgatct gattgccaga aaacaggaac ggacgatcgg
    61021 agcagttgaa cgtttaggaa acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt
    61081 tgataactct aatatcatgg ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg
    61141 aaaaccggca agaaaagaat atcggaaata taaaataaaa acagtcaacg gaccggatga
    61201 ttatgcatca atgagagaag tcatctatcg cagatattca cgagtgattc gcgaagggtt
    61261 gcctttacct gatttgatct tgatcgatgg aggaaaagga caagtagatg tggcaaaaga
    61321 tgtcttagaa aatcaattag gaatcgatat tcctattgct ggcatggcaa aaaacgataa
    61381 acataaaaca aatgagttgc tattcggtat agatttggct gtagtaccgc ttgagcgaaa
    61441 ttcaccagag tttttcttac tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac
    61501 tttccatcgg cagctacgca gtaaaaacag ttttgcttcc cgattagatg aaattgaagg
    61561 gttaggacca aaacgaaaaa aaatgctgct taaagagttc aaatcgttga aaaatatcac
    61621 agcagccacg acagaagaat tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt
    61681 atttgataaa ttacatcagg aatgaggaaa gggcacttta caaagaaagt aaagtgtcct
    61741 atcattttat gtacaacaaa aagagcgttt acgttttgtt ggtaaataat aaatcgctta
    61801 cgtctggtgt ctaaggatat atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt
    61861 caaggatctt gttaaaactt ttgggaacta cacagctgtc gatcatgtct ctttccaaat
    61921 ccccgatgga aaaatcatgg ggctaattgg ccaaaacggg gctgggaaga caacgacttt
    61981 tcgtttgatc ttggattttt tgacacaaga ccaaggagaa gtgttatgga atggccatca
    62041 tcttggggag aaagattatg atgtcatcgg ttatcttccg gaggaacggg gactttatcc
    62101 gaaagtaacg attcaagacc aactgcttta ttttgcagaa cttagaggga aatcaagaaa
    62161 agagattgaa ccaaaaatcg atttttggat ggaaaaattt caggtaaaag ggaaaaagac
    62221 ggataaagta aaatctttgt ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt
    62281 aatccatgaa cctaaattga tcattttgga tgagccattc agcgggttag atccggtaaa
    62341 tgctgaattg ctaaaagatg gaatattgga attgaaaaaa catggttcat gtgtcatttt
    62401 ttctagccac aatatggata atgtagaaaa aatctgcgat catttgatca tgttgcgaaa
    62461 cggaaaaatg gttttgaatg gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa
    62521 gttattcttg gaatctggtt taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa
    62581 agtgatcctt catgaagaca atacgttaga gattacctta gaagatcctg aagctgggaa
    62641 agaaatcttt actcgtgcga ctcagttcgg ctacattcct atgtttaacc agcaaccgcc
    62701 aactttggaa gagattttca aattgaaagc aggtgaaatc gatgcgtaag ttttggatta
    62761 ttaccaaaga tgtctattta aaaaatgtta agtcgatttc tttcttgatc atgatattag
    62821 tcccttttat catgctgggt atcatttatg tagcaggaaa ctttgctcaa caaaatagtg
    62881 atgtcaataa aatcggcgta ttggctgata atgagcaaat cagtcagcaa atgagccaag
    62941 tcaaaacaga tgattttgag ttcaaagcag ttgattctga agaaaaagca caaaaagagc
    63001 tttcggaaga aaaaatcgat gcgtatttag tagtctcaac tgagaatgat aagataaatg
    63061 ggatgcttta tagcgaaaat tcattaggac aaagtacaca actactgata caacaacaac
    63121 tgactggctt gcaatcgatg cttcgagcaa gtcaattagg catttcacca gaagaagtag
    63181 ctagcttgag ccaacaagca agtttgactc gacaaaaagt caattttgat tcaaaaggaa
    63241 aaatgacagt tggagaagat aactcgacag tccaatatgt cgtaagttat gtagcaacga
    63301 ttgtcttgtt cattattatt ttgacgtatg cgcagatcat tgcacaagag atcgcttctg
    63361 aaaaaggcac gcggatcatg gaagtgatcc tgtccagtac gactgcacaa aagcattttt
    63421 acggaaaact aacaggtgtt ttacttgttg cattaacgca aatggcatta tacggtatta
    63481 tttttggtat aggatacagt caattcaaag atatggaaat ggtcaaaaat atattagacg
    63541 gtatctcatt ggataacttg attggaccat tcttgtggta ttctcttcta ttcatgatct
    63601 ttggcatttt gattttttct gttttagcag ctttatgtgg ctctttggtc aacaaagcag
    63661 aagatacagc aaaagcaatt ttgccagtga cttatctatc tctaggcgga tatatgcttg
    63721 gattgatact aggagcttca gatccaaaca atatcgttat tcggatcact tcgtatattc
    63781 cattcttatc ttcatatatt atgccggttc gcttagcaaa tgagacagtc ggtatgggtg
    63841 gagcattgat ttccttgttg gtacttgtag ccgtaacatt cttattgatg atcttgtctg
    63901 caaacatgta caaatcgaat gtactcgtgt atagcgaagg cggattatgg agttcattga
    63961 aacaatcgat ttcaattatg agaaatgaac gaaaaaaagc ataaaatata gaaaaatata
    64021 gaaggaagct ggacatttat tgttcccagc ttcttttttg tatagaccta tttctatttt
    64081 tttattatat tggatatata tattcattga aaatagagca taacaacttt ataatgttga
    64141 gtataccaat ttttggaggc gttttaatgg accgctattt agatttactt tcagagaagt
    64201 tccctacaac ggaagatgtc atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc
    64261 caaaaggaac agagttattt ttgagcgata tacatggcga atttcctgct tttgatcata
    64321 ttctgcgtat cggttcagga aatcttaaag aaaagatcaa agaccttttc tctgaacaaa
    64381 tgacggaaac ggagatcagc caattcacta tttttacggc ttatccagaa tatgtgttga
    64441 cgactgattg gtacaaaaaa caagataaat cccagctgat ccatcgtttg atcgatcttt
    64501 tacggtttac gacagtcaaa tacactcgat caaaagttcg aaaaggattg ccgaaagaat
    64561 atagctatat cattgaggaa ctcttgtata tcgatgatcg tatcaacggg aaaaaagact
    64621 atgtaaaaaa aatcattgaa caacttgtct tgatgaagga agaagaaaaa tttttaacaa
    64681 aacttgcgca gacgatccaa aaatctgtca tcgatcattt gcatatcgtg ggtgatattt
    64741 ttgatcgagg aactcaagca gccaaagtaa tggatcgaat catggatttt tcctctgtag
    64801 atatccagtg ggggaatcat gatattttgt ggattggtgc gtattgcggc tctgaggcat
    64861 gtttattgac gcttttacgg attgccgcac gctacaacta tttatttgaa ttagagaaag
    64921 aatatggatt aaatttacgt ccattatttt cttttgccca tcaaacctat cagaagaatc
    64981 cagtatttac ccctaaaaac aggaaagatc tatctgaaag agagcaagaa gaattagaaa
    65041 agatccatca agcactttct atcctccaat tcaaatcaga acatcagttg ttgaatcgac
    65101 gacctgaatt taaaatggac gatcgtaaat tgttggaaaa aatcaactat gaggaatcaa
    65161 cgatcgtttt agaaggtcag ctctatgagt tgaaagggac ttgttttcaa acgatccagc
    65221 cagatgatcc taaaaaattc ttggaagagg aacaaaaagt gatcgatagt ttgatgtatt
    65281 cattccagca ctcgttaaga atgcaaaaac acatgacgtt tttgatcgaa aaagggggaa
    65341 tgtatctcac gaacaatcag catgtactgt tccatggctg tatacctgta gatgaaaagg
    65401 ggcagctctt agcatttgaa ttagaccaat cttatcgcgg aaaagaactg ctgggatttt
    65461 ttgaaaaaca gataactgaa agcgcaaaag atttgactgc aaatgaagat ttggcgaccg
    65521 atttgatttg gtatgcgtgg agcgggccat tttcgccttt attcggcaag agtaaaatgg
    65581 caacttttga gcgctatttt ctcacagagc ctcatacgca cgtcgaaaaa agcaatccct
    65641 attatcactt gcgagatgaa gagtggttca gtgaaaaaat tttggcagaa ttcgatgcaa
    65701 aagaagaagg ttcagcaatc atcaatggac acacgccagt aaaagtaaaa aaaggagagt
    65761 caccgatcaa agcaaacgga aaagcatttg ttattgacgg aggtctatcc aaagcttatc
    65821 aaaaaacaac cgggattgct ggctactcat tactatgcaa ttcttacggt ttccaaatcg
    65881 tgacacatta tccgttcttg cctattgaac aacaatttgc aaaaaaatct gatcaaacga
    65941 atgtaaaaaa agtgatcgag cagcagctcc cacgcaaact taatcggaat acgacaaaag
    66001 gaatggaatt acagcaacag attactcaac tccaaaggct gttggattat cgaaaggacg
    66061 attgagaaaa atgctagcca acattgctca atagactaaa aagcgactgc aacaaaaggc
    66121 gtgtcgcagt ctccttcgga attagctcaa aaaacggtaa aatattagaa gctatttttc
    66181 tgtttatttc tctaatactc aggtcagggc gctgatttca cgacctcttc cttaaacggt
    66241 gttctcctgc caactcctcg gtcacaagtc acaatatcca acaaacatga gaagctgttt
    66301 tttgaaattg ctccttgtga ctcagagttg aacggcaggc tcacgaccta atcagaaaaa
    66361 cgattaaaat tcattcacaa agaaatcgaa taaacgctgt tcagatgaat ctacacgatg
    66421 agaaagttcc ggatgccatt ggacaccaag gattttgtgc atattatctt tactttggac
    66481 tgcttcaatg agtccatcgt tggaaaaggc gatggctttt aacaaaggag aaagatcttt
    66541 gactgcctga tgatgatagg aattcacaaa gtatgaatca gggagaaggt cacttaataa
    66601 actgtccgaa acgattttga cttcgtgagt tgcaaactgc ggggcagtgg gctgttgttc
    66661 gtgttttacg gaccattttg gatataatga aagatcttga taaagtgttc cctccaacac
    66721 gacattcaat agttgaagtc ctcggcagac tgcaaaaatc ggttttcttt gtttcaatgc
    66781 ttccaagatc aatgcctgtt cgaattggtc tcgttgaata tttgtttccg tcagttttgg
    66841 atgagggtct tccatgtaaa gttgcggact aacatcctga ccgcctgcaa gaatgagttt
    66901 gtcgattttc tcaattagtt gaggcgctga atctggagac atgactggca gaataagagg
    66961 taatccttga gcatctaaaa ctgcatccac aaatccctgc ggtgtatagc ttacttgatt
    67021 tccttggaaa gtatctgtcg ctcgaatcag ttgattcccg gcaatgccga tgattggttt
    67081 catttttatt ccccctaaag tagactaatt gtaacaagga tttaaaaaaa tttaaactat
    67141 gtaagacttg cccctatact tatctgttct tgctgtgtta ctatctgtaa agagaatttt
    67201 cgtgtgaaat tcatgacgat agttgaaaat attgcttaat ttgttataat aaaaagtaag
    67261 cagaaataaa aaggttgggc gtaagcttca aggatctagt tcttcaaggg gtgagaagga
    67321 cattttattg aaggggcctg cctatactaa gttatcttta gaggtacata ctttcaatga
    67381 aaaaagaaag catgtacctc ttttttaaat cgaaaggaag tgaagcaagt gttagaagct
    67441 ttggaacaaa tccgaaaagc agaagaagaa aatgagaagc gtgctcgaac cctacggcag
    67501 cagttgaaga cctatgcaga agaaaaagag caagaattgg agataatcca aacgaacagt
    67561 cggcaacagc tgaaacagtt ggtagaagaa aaagaacaat tcgaaaagca agaattagaa
    67621 aaagaaaaaa tgaaattgaa agcagaagca gacaaggtgc aagcaacttt gaaagaccaa
    67681 tatcaaaagc atcagagtca agcaatcggt gcaatcattg aaagggtgaa agaaacttat
    67741 ggccgtcact aagatggaaa aagtgaccct catttcagat aaaagaaatc aggagaggat
    67801 tttacaagca gtacaaggaa tccaaaatgt tgaaatccgg gatctgtatc aaaatagaac
    67861 aaacaaccag tgggtagaaa aatattttcc agataccacg attattgata aagaagcaaa
    67921 attaagcgct ttaggcaatc ggctgaaaga catccaagaa gctatccaat tcatacaaca
    67981 ccacggcgat agccgtcaaa agaaactcca tttgaaacgt caagaacttt ccttacaaga
    68041 cttggaagca aattattccg aagaaaattt catgaataaa ttagaagaag tccttgattt
    68101 aaaagaaaag tgggaacagc taactgaaaa acgtgatcgt ttagaagaag aggaagaatg
    68161 gcttcttcat tggcaacagc tggatattgc tccaagtatg tatgcaagta agacgaccat
    68221 ttttgagatg gggactgtga gtgcagcaaa ttttgaaagt ttcaaggaag aactaagtaa
    68281 gcttgatgac gtttatatgg aagaaataga ttatacgcca aaacaagttt atctttcgta
    68341 tgttgcttta aaaaaatcag ttgctgctat agaagaaatt gctgggaaat acgggttcaa
    68401 taaagaaacg tatccttatg aacaaaatcc taaagaaaaa ttgcaagaaa ccaaacagca
    68461 attatccatt gttcacgagc agcaaaaaaa gataacgaca gcgttaggtc agtgcagcgg
    68521 ctatatccaa gaatttgaat gggtagaaga agtcacactt gccgttgcag aacgggaaaa
    68581 agtaaaagaa cggtttatcc atgccactta tctgatcgtt ttgcaaggtt gggtagatgc
    68641 agaagaaaaa cagaatttga tgcatgtgtt aaacgaaact gtatcagaag aagatatata
    68701 cgttagtttc gaaacaccaa actcagaaga gattcagcag gaagtcccta ctaaattgaa
    68761 aaaccatccg cttatcgaac cttttgaatt gctgactgaa atgtacagtc tgccaaaata
    68821 tgaagaagta gatccaacgc cttggatgac accattttat cttgtcttct tcgggatgat
    68881 ggtcgcagat gttggttacg gattgctgat gctcattggg acaatactag ctcaaaaact
    68941 atttgtttta ccccgcggga tgaaacgttt cgtaaaattc tttgagattc tctcggtacc
    69001 atcgatcatc tggggattga tctacagttc atttttcgga caggcattac caaaagaact
    69061 atttggtatc cgcttgcctt tcccaatcct atctacgacg gatgacgtca atacgatttt
    69121 gattttatcc gtgattttcg gtctgataca gatcttggtt ggattattcg tggcagcaaa
    69181 ggaaaatatc aggaggaaag attacttgag tgctgtcagt gatggttttg cctggcaagg
    69241 gatcctgatt gggatcgtta ttgcattgat tggtgtcatg ttgataaaaa atacagcatt
    69301 tgtttatctt ggcgcggggc tatcgatcat tgctgcttta tgtatcttaa tcgtgccgat
    69361 tatccaatcg ccgtctaaag tcaagggtgc tgccaaagga gcgtataatc tttacggatt
    69421 gactggttat atcggtgatt tagtcagtta tacacgtttg atggcactag gtatttcagg
    69481 cggaagtatt ggcgcggcat tcaatatgct agttgctttc atgccgccag ccgctcgatt
    69541 cagtgtaggg atccttttga tcatcgcttt acacgcactg aatatgtttt tgacactgtt
    69601 aagtgcctat gtccatggtg cacgattgca gtatgtcgaa ttttttggaa aattttatac
    69661 aggaggcgga cgtgcctttg acccgctgaa aacggcggaa aaatacgtca acatcaatca
    69721 taagaaaaaa aacaataaat aattggagga tttttataca atgatggatt acttaattag
    69781 tcaaaatggt ggaatggtat ttgcagtatt agcgatggca acagcgacga ttttttcagg
    69841 gattggttca gctaaaggtg ttggaatgac aggtgaggca gcagcagcgt taacaacaag
    69901 tcaacccgaa aaattcggac aggctttgat tctgcaattg cttccaggta cgcaagggtt
    69961 gtatggattc gttatcgcct ttttgatttt tatcaactta ggaaatgata tgtcagttgt
    70021 acaaggattg aactttctag gcgcttctct gccgattgct tttacaggtt tgttttcagg
    70081 tatcgcccaa gggaaagttg cagcagcagg tatccaaatt ttagcgaaaa aacctgaaca
    70141 tgctacaaaa ggaatcattt ttgctgcgat ggttgaaaca tatgcgattt taggtttcgt
    70201 tatttccttc ctattagtat tgaatgccta agcataaagg aggaaaagca gatgaacgcc
    70261 attgaaaaaa tcatctctca gatgaatgaa gcagcagagc aagaaagagc ggcgttagag
    70321 caagaagagc gaatgaagat cgatcaaaac tttgaacaaa aacgtacgca aatcgaaaca
    70381 gaacatcaaa aacaaaaaga aaaacaaatc gaattacttg aaaaaaaata ccgtcagctt
    70441 aggaatcgcc aacaagtaga agtacgacaa gaaaatttga atgcgaagca ggaattttta
    70501 cgacgattgt ttgcagatgc aatagctgaa atggagaatt gggatgaatc tgaacaaatc
    70561 cagtttatca aaaattcatt gtattcatta cctttgacag gcaaagtaac attcattgct
    70621 ggagaaaaat cagcagctta tctttctcag acattgctgg atgaatggaa caatgaactt
    70681 ccctttatga tggttttaag tgatgaaact gttgctgatc aagcgggttt tctgataaac
    70741 gatcaaggag ttcagtataa ttttcttttc agcagccttg tacaagatat ccaaggaacg
    70801 atgagttttg agattgccaa tcaattattc gaataaggag ggatcagtgt atggaatacc
    70861 acgaactaaa tcccttgatt cgcggaagag agcttgaact tttgtccaat gagacatttg
    70921 aacggatgat tcaaacaaat tctgtcgaag cacttggtga aatattgaaa tccacgattt
    70981 acagtccata tatttatgac ggatatgaat atgacttcga acagaatctt ttaaaggagc
    71041 agagccgtct atttcgctgg ttaaaggaat cagcaccgga gcctgaaatc gtttggatct
    71101 atacgatgcg ctatactttc cataatctga aggttttgac aaaagccgag atgacaaatc
    71161 aaaatctgga ccatttgtat atcgacgatg gattttattc attagaaacc ttgaaggatg
    71221 ccattcatac gcaagtttca aatgaactcc ctgaaagcat tatggacagt atacgagaag
    71281 tacgtgatta ttttgaagag tctaccattc ttcagggaat tgacgtgatt tatgatcgac
    71341 agtttttgac agagcagcga cggcttgccg ataagctagg atatcaagaa ttgctgacag
    71401 aagtcattgc ttttattgac ttgaccaata tcacaacgat ggctcgagga atattgcaga
    71461 aaagaacata tgggttcatg acgacggtac tttccagctc gggaagtatc gaaaaagaag
    71521 tatttttgaa ctttgtagat aaagatatgg aagattacac gaattttcta ttagagacag
    71581 attatgctga ttttctgcgc cctgtcatcc gtgagggaaa tcttgatctc gtaaagcttg
    71641 aacagttgaa agatgattat ctaagttcat tgtatcaaga agcacaaacc caggctttcg
    71701 gaccactgcc actactagcc tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt
    71761 tactggttat cggcaaaaga agccatttcg aaccagaaac aatcagaaag cgggtgagac
    71821 aagtctatga cgcataagat cggtgtagtt ggcgacaaag attctgtttt gccattcaaa
    71881 ttgtttggct ttgatgttcg ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa
    71941 gaaatggcaa agaatgagta tggcgtgatt tatgtcacag agcaatgtgc agcattgagc
    72001 ccagaaacga tcgaacgtta caaggaacaa atgatacctg ctatcgtatt gattcccaat
    72061 catcaaggat cattagggat agggctggaa gaaattcaaa aaaacgtaga aaaagcagta
    72121 ggtcaaaata tcttatagta tgaaaaggag atagaaattt tgcaaattgg aaaaatcata
    72181 aaagtctccg gacctcttgt aatggctgag aacatgtctg atgccagtat ccaagacatg
    72241 tgtctagtgg gagatctagg agttatcgga gaaattatcg agatgcgtgg cgatgtggcg
    72301 tctattcaag tatatgaaga gacttctgga attggtccag gcgaaccggt tcgctctact
    72361 ggtgaagctt tatctgttga attaggacca gggatcattt cacaaatgtt tgacggaatc
    72421 caacggccat tagatacctt tatggaaatc acacaaagta atttcttagg gcgcggagtt
    72481 caattgcccg cattggacca tgagaaaaaa tggtggttcg aaccaacagt agaagccgga
    72541 gaaacggtga gtgctggaga tgttatcggg atcgtagaag aaacaaaagt tataaaacat
    72601 aaaatcatgg tgccaaatgg gataaaagga acgatcaaaa caatcgaaaa aggttcattc
    72661 actattgatg aaacagtatg tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg
    72721 cttcaaaaat ggccagtaag acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca
    72781 cccatgatca ctggacagcg agtgattgat acattcttcc cagtgacaaa aggaggagca
    72841 gcagctgtac ctggtccttt tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa
    72901 tgggccgatg tggacatggt tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg
    72961 gatgttttaa atgaattccc tgaattgatt gaccctaata caggagaatc gttgatggaa
    73021 cgtacggtat tgattgctaa tacatcaaac atgccagtag cagctcgaga agcttctatc
    73081 tatacgggaa tcacaattgc ggaatatttc cgtgacatgg gttatgacgt tgctatcatg
    73141 gcagactcca cttcacgatg ggcagaagca cttcgtgaga tgagcggacg tttggaagaa
    73201 atgcctggtg atgaaggtta tccagcttat cttggttcac ggcttgcgga atactatgaa
    73261 cgatctggaa gagtcattgc attaggatca gaaggtcgcg aaggaagtat aaccgcaatc
    73321 agtgctgtat ccccgtctgg aggggatatt tcggaaccag tcacacaaaa tacattgcgg
    73381 gtcgtaaaag tattctgggg tcttgattcc agtttagccc aaaaaagaca ttttccttcg
    73441 atcgattgga tacaaagcta ttctctttat tcaacagaag tagggaagta tatggatcat
    73501 atccttcaag ctgactgggc atccatggtc accgagggca tgcgtatttt gcaggaagaa
    73561 cagcagctga atgaaatcgt gcgtttggta gggattgatt ccctttcaga taatgaccgt
    73621 ctgacattag aagtggcaaa atccattcgt gaagattatt tgcagcaaaa tgcttttgat
    73681 gatgtcgata cattcacttc aagagagaaa caattcaaaa tgttgaagat gatcttgacc
    73741 tttggtgaag aaggacgccg tgcgttgtcg ttaggtgctt atttaagtga gatcatggac
    73801 ggtaccgtat cgattcgtga aaggatcgga cgaagcaaat atatcccgga aacagaatta
    73861 gaaaaactag atcaaattat tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa
    73921 ggaggcatga gcaatgatta aagaatatcg tacgatcggt gaagtcgtcg gtccattgat
    73981 ggcggttgaa aaagttgctg gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa
    74041 tggcgagatt cgtcgtggac aagtattgga agtccaagaa gataaagcaa tggtccaaat
    74101 attcgaagga acaagtggta tcaatttacg tgactcttct gtacgatttt taggccatcc
    74161 tttagaacta ggtgtatcgg aagacatgat cggtcgagta ttcgatggtt tgggacgacc
    74221 gaaagataac ggaccagaga tcctgcctga aaagcatttg gatattaacg gagaagtaat
    74281 caatccagta gctcgggatt atccagatga gtttatccaa acaggtatct ctgcaatcga
    74341 tcatttgaat acattagttc gtgggcaaaa attaccagta ttttctggct ctggtcttcc
    74401 tcataaagaa ttagctgctc agatcgcgcg acaagcaacg gtactcgatt catcagatga
    74461 tttcgccgta gtctttgctg ctattggaat cacttttgaa gaagctgaat ttttcatgga
    74521 agattttcgt caaacgggcg caatcgaccg ttctgtcatg tttatgaatt tagcaaacga
    74581 ccctgcaatc gaacgtatcg ctactcctcg tatggctttg actgctgcag aatatcttgc
    74641 ttatgaaaag ggaatgcatg ttttggttat catgacagac atgaccaact atgcagaggc
    74701 attgcgagag atttctgcag cacggcgaga agttccagga agaagaggat atcctgggta
    74761 tctttacacg aacttagcta ctttattcga acgagcagga agaatccgcg gactaaaagg
    74821 atcggttact caaattccga ttctaacaat gccagaagac gataaaactc atccaatccc
    74881 tgacttgact ggttatatta cagaaggaca gatcatcttg actagagagc tttacaagag
    74941 tgggattcag ccaccaattg atgtactccc gtctctttcg cggttaaaag ataagggaac
    75001 tggacccgga aagacaagag aagaccatgc agctacgatg aatcagcttt ttgcagccta
    75061 tgcacaagga aaacaggcaa aagaattagc cgtagtttta ggtgaatctg ctttatctga
    75121 tgtagacaag atctatgcga agtttgctga gcggtttgaa aaagaatatg tcaatcaagg
    75181 attctacacg aaccgttcga ttactgaaac acttgatcta gggtgggaac tgctatcgat
    75241 gttgccaaga accgaattaa aacgaatcaa agacgacatg ctggacaaat atctgccaga
    75301 agggaagtga gttctgatgg cacgattgaa tgtgaatccg acccgcatgg aattgactcg
    75361 attgaaaaaa cagttgacaa ctgccacaag agggcataaa ttattaaaag ataagcaaga
    75421 tgaactgatg cgtcaattca tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat
    75481 ggaagagtct atgacagaag caatgtctga ttttgtattg gcaaatgctt cgatcacgga
    75541 acctttcatc gaagaattat ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga
    75601 caaaaatatc atgagtgtaa aagtacctgt gatgaatttt gaatatgatg aacagctgac
    75661 agacacacct ttggaatacg ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga
    75721 tcggtttact accctgcttc caaaactttt ggaactgaca gaaatcgaaa aaacttgtca
    75781 actgatggcg agtgaaatcg aaaagacacg tcggcgagtc aatgcattag aatatatgac
    75841 gatccctcaa ttagaagaaa cgatctatta tattcgtatg aagctggaag aaaacgagcg
    75901 cgcagaagta acaaggttga tcaaagtaaa aaatatgggt accactgact aagtggtatc
    75961 tttttttaaa agaggaaaac gatgattaaa caaagcaaac aaataaagaa acgtttgccg
    76021 gctgttcagc ttctcgcatt aggatttttt ctcctgattt tagttggcgg cggcctactt
    76081 accctaccat tttttagtaa tagtgggcat gggacaaatt ttatcgatgc gcttttcact
    76141 gcaacttctg ctgtctgtgt aacgggattg acaacattga atacagcaga gcattggaac
    76201 agtgctggtc aatttttgat catgcttttg attgagatcg gcggtcttgg ttttatgatg
    76261 gttccgattc tatgctttgc attgataaag aagaaagtca gtctcagcac acgtattata
    76321 ttaaaagaag cgctgaatct agaagaagta tcaggtgtga tgaagctcat gctttacatc
    76381 ctaaaattgg cctttgttat ccaattgatt ggtacggttg ctttatcttt tgtatttgtg
    76441 cctgaatttg gctggttgaa aggtatctgg tacagtttgt tccatgcaat ctctagtttt
    76501 tgtaatgcag ggtttgattt gttaggagac agtttagcca atcaccagca aaatgtttat
    76561 ttgatcatgg tggtatctgc tttgatcatt tctggtgggt taggcttcat cgtatggcgt
    76621 gatttactgg aatatcatca gaaaaagaag atgacgattc attcaaaaat cgcattaacc
    76681 gttacaggta cccttttatt aggcggattc gttgtgttct ttttcacaga gcgcaatggg
    76741 gcaacgctag tggaagggaa tatgtttcaa cgattggcaa acaccttttt tatgagtgtg
    76801 acacctagaa cggctggata ttattcagtt gattacctcc atatgagcca tgcaggtctg
    76861 attttaacga tgtttttgat gtacatcggc ggaacatctg ggtcgacggc cggcggattg
    76921 aaaacaacta cgttaggtgt gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg
    76981 agagcggaag catttggccg taccatccga caaggcgcag tgttgcgggc acttacttta
    77041 ttttttgtta cgttgacgtt gtgtgtagtc gccatcatga ttttatcggt gactgaaacg
    77101 atacctaaaa cttctggcat cgaatatatt gcttttgagg tcttctctgc gtttggtaca
    77161 gtaggtctta cgatgggatt gacgccagac ttgactgcaa tagggaaatt gatcattata
    77221 gcgcttatgt acattggacg agttggtgta ttgactgtgg tcttctctct gatgacaaaa
    77281 gcacaaagaa cacaatcaaa atacaaatat ccagaagaaa gtgtcatgat tggataattt
    77341 catcttctga cagaaaccta gtgatttagt aaaataagaa aataaaataa tttttcttgc
    77401 ttcttggata ctttaagtgc aaaatgttcg tttatttttt gaggcactac ccgttgttga
    77461 acctcgggta gtgcctcttt tttattgaaa ggaatcaata agatttgatt cctttattta
    77521 tcaataggtt ataattcata tttttttatt ttttctttta cgatacttag tccagaagct
    77581 aaaatttcaa ggttattaga ggatagggaa attcgaacga atgcatctgg ttgcgcacgt
    77641 gtagtaaagc gtctggaatg ataaagtcgg ataccgttag caagaaagtc attctcaatt
    77701 atcttagggt caatctcagg atcaactgga agaattcgat agtaagggac acgattcgtt
    77761 ggtttttcta ctttgaaaag agaatcaaaa agctgattgg ctctttttgt aagacttaat
    77821 ttagtttcta agatcctatt tgcttcattt gaatggagga tttgagtgat gacttccaca
    77881 tctaatccag aagttttcac atttatatta aaaattgctt gttctatctt gctttggaca
    77941 tgtactggaa ctacaagata agcaactcgc aatccagaac aaataaattt tgtcattcca
    78001 gctaagtaga ttgtttggtt aggtaataaa ttttggaaag gaggaatgat cgtatgttga
    78061 tagtaagttg ttaaaaatga atggatatca tcttcaataa cccatagacc ttctttttca
    78121 atcacttcgg ctaattgctc tcgtcttttg tgagagattt gaaaccctat aggattatta
    78181 caagaaggca taagaaagac accatgaatc ttcttttttc tacattcctg aatcaatata
    78241 tcaggtcgca ttccttgctc gtcaaaatca attggaacga tctctaaatg atataactga
    78301 gccaattcaa tgaaattagc gtatgtatat cggtctgttg ctattctatc acctggagag
    78361 aaaagtgcag aaagtgtgat agccaatcca ttttgaacac cggaaacaat agatagagat
    78421 tctaaggacg gaatgactcc ttgagttttt aaccaatttg ctgcagtagt caattgaaaa
    78481 tttgtgcctt tgggatcttg gtaattaagc aaaccaacaa gatttgggtt attactagtt
    78541 gattgaataa acgggacaac gaatttatta cattcttcaa aagaactaac cagaccaaaa
    78601 tcaatgatat gttcggttac ttcattggta gagatggtga tggactcaaa gacattggga
    78661 gaaataaatg tgccacttcc aatattcgta tacagcaaac ctttctcaat tccatattta
    78721 taagccttgc caacggtagt aaaatttaaa tctaaataat cagctagctc tctttgggag
    78781 ggtaactgag tgtttttctg taatttattc tttaaaatat cttgttctaa ttgttgaatc
    78841 aacgatcgat aataaggacg cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa
    78901 ttgtcataag aattaatagg cattttctcc actccttaaa attgtatacc atacaatttt
    78961 accattgatt gtattgattt tagaagttaa tcttttgtga ggagggaatt gtctatgaaa
    79021 attattaatt ttgaggaagt agtagcttct tttagttttg aagaagcgat tcaagtaatg
    79081 aaaaaatgtt tttttgatta tgaaagtggt aaaatcagcc aaaatcctcg attggtggaa
    79141 attttacctg acggaaaagg caaaaatatg tttgcgatga tgcctgctta tttgggggag
    79201 aacagatatt ttggcacaaa agtcattacc gcttttccgg ataatcatca gtatcattta
    79261 ccttctcatt taggagagat tttcttgttt gatgctcaaa acggactacc caaagcattg
    79321 ataaacgcaa atgcagtcac ctggattcga acagctgctg tatctgcgct ggcaacagat
    79381 tacttagcaa atccgcatgc agaaacactt gctttaattg gagctggtca acaagcattg
    79441 tcgcatttga ctgcgatttc taccattcgt cccatcacta cagtgtttgt ttacgatatt
    79501 ttagaagaaa gacgaaatac ttttatcgct aaagctaaaa aacagtatcc taaaataacg
    79561 tttataaatg caatatcagt agaagatgca gtacgtccag cggaaatcat ttgtacgttg
    79621 acctctagta aagaggcatt tcttcaaaaa gagtgggtaa gggaagacgc acatatcaat
    79681 gctgtgggca catttactcc tgatacaaga gagataagta catcgttgat ggaaacttct
    79741 caaatctatg tagatgatta ctcagctgcc ttaacagaaa gcggggacct gcttatagcc
    79801 atggcagaag gagatttaga ggaatcttgt attgttggta cattaggcga attagtttct
    79861 gagaaaaaaa cattttataa aggagaatcg aggccgacga ttttcgacgc agttggtcta
    79921 gctattgaag atctttgttg tgcagaatat atttataaca aattagagaa aggggcttta
    79981 aaatgaactt agattattac gtagttgatg catttgcaga tgaggtgttc aaaggaaatc
    80041 cagcagcggt ttatgtgcta gaagaatggc tgccagaagg tacgatgcaa aaaattgcca
    80101 ttgaaaacaa tttatctgaa acagcattta cagtgaagaa aaaccaagaa tttgagctta
    80161 gatggtttac gccagatcga gaaatcgatc tttgtggtca cgcgacatta gcaacagcat
    80221 ttgttttatt taactattac aaaatacctg atgaaacgat taaattttct actcaaagcg
    80281 gtaatctatt tgtgacaaga attaaagatt attattacat ggattttcct agtattatgc
    80341 cgaaaaaagt gccgatattg tctgaatatg aagaagcgat tggggctaag attaaagaag
    80401 cctacttagc acgtgattta tttttcgtat tagaagatga aaaaacggtg gctaaattgc
    80461 agccagattt tacagctatc aaaaattttg aattaggaat aggagtcatc gtgacagcag
    80521 aaagtgtgca aaaagacttt gtttctagaa ccttctttcc aaaattaaca atcaatgaag
    80581 atccagtatg tggatcagca cattctaatc ttattcctta ttgggcagaa agaatgaata
    80641 aaacaaaatt gtcagcttat caaatgtcat caaggggcgg ggatctagaa tgtgaattaa
    80701 aagaagatcg tgtgataata ggaggaaaag cagttttgtt ttctaaaggt acctcgttta
    80761 tttgatttct ttcaaatgaa ttaaaaataa attggtggag agtggcaacc ttatagctac
    80821 aatggttgtt aaaaaaagac gcaaactttt tcaatttaat gaaaagtttg cgtctcattt
    80881 taattattgg tgtttgtttt ttattcaatc aagagtaaaa tgccccatac taactatcct
    80941 cggatgactt cgattttata accatcaggg tcaatgacaa agtagtaaga aggcgcagtg
    81001 ccaggtaatc cttttaagtc agtaatctca agaccggcag ctttatgttg ctcgtgtaat
    81061 ttttctagat catcagctgc tatagcgata tgaccatagc cgtctcctag atcatacgca
    81121 ccatgatcat agttgtaagt caactctaat tcatagtcat ccccaggaag tgtcaaataa
    81181 acaagtgtaa atttgttttc tggaaaatca cggcggcggc tttcttcaaa accaaatgcc
    81241 ttttgataaa attcgattga tgcttcaaga tctttcactc gaacacatgt gtgagccatt
    81301 ttcatagatt catctctcct tttcctttta ctcaatcata acaaaaagaa gtaaggaaat
    81361 agttgatttg tgcttgttca tgagaaattt ctcatgtaaa atggatagaa aataaaactg
    81421 ttaaggtgga aactattcat ggataaacca atttttggtg aaaaaaagca atttgctgat
    81481 tatcaaaccc gttatgcagc atatattgtc atagaaagag atcagcagat tgcagtaatc
    81541 gaggctccaa atggtgcttt ttttcttcct ggcggggaaa ttgaaggaga agagacgaaa
    81601 gaagaagcga ttgtccgtga gctgatagaa gaaatgggaa ttgtggcaga aatttcttct
    81661 tatctgggag aagctgatga gtatttttac tcgaattatc gtgcaactta ttattataat
    81721 ccaggatatt tctttgttgc tgattcttgg aagcgtatag gagaaccaac tgaaaagacc
    81781 aataaaattt ggtgggtgag ccctcaagaa gcgcttgtga aattgaaacg tggtagccac
    81841 cgatgggcag tcgagagatg gatcgaaaaa aataaaaact gaaagaaagc gagtagatca
    81901 gctttctctc agttttttta tttattacag tacatatttt ttgattgctt cagctacacc
    81961 gtgctcgtcg tttgatgaag tgatgacatt tgctgcttct ttgacaagag aaacggcatt
    82021 tcccattgct actcctagac cagcatattc aatcatggat aagtcgttct cgttatctcc
    82081 gatagccatg atttcatctt gatcaatacc aaggtgctgt gcgagattcg caactgccgc
    82141 acctttactt gcttctttat ttagtacttc gaagtaatag ggcgcacttt tgaccgttgt
    82201 gtatttatca cggaaagttt gcggtaaacg cgcaattgct gcatctaaaa tttctggttc
    82261 gtcgatcatc atcattttga cgatgttcat gtctggtgtc atttcttcag gtgttcgata
    82321 ttttagcggc atcttgacta aacttgcttc gtggatcgta taagcgctga tatctcgatt
    82381 aggcgtgtag atcgctttgt catcgatcga atggagatga ctgccgacac ggcgtgccat
    82441 cacttcgatc tctaggaaat cttcatgtgt cagtccgtga cgggcgatga ttttacctga
    82501 tttcgtttgt tgtacgagtg ccccgttata agtaataacg taatcattat cttgaaaaag
    82561 gttcaactcg tttaactgtt cctggacacc tggtaaggga cggccagtgc ataaaacgat
    82621 cgaaacacct tttgcaacag cagcggcaat cgcttctttt acttcttttg ttacttctct
    82681 tttctcattt aataaagtac catcgatgtc gatagcgact agtttgatag acataatttc
    82741 ctccaatata ttttaattta tttcaatcaa tgcgccatta cggatatggc tggcaaattc
    82801 ttgataagtt tcgtcaaata aatcataatg atcacgcaag gacggatcaa ccatttcttt
    82861 tggaaagaaa aatcgctcat ctccttgttc ttgtcccgct aatgcagcaa caagctgact
    82921 aacctgtgat aattctatca aagttccgtc atttcgcaaa atctcgattt gtgtgcgatg
    82981 tctgccttgt tctgggcgat aaaaatcata aggtagatcg tagctagaat tcacagcagt
    83041 gtaataaaca ggattatatc cgaccttttc tactaacaaa gtgagttctt ggactaattt
    83101 tgaatcccgg ttgccagaaa aagtagcaga tttcagtggt ttacgattca aaaaacgttt
    83161 agctaaatca tttaaaatag gatcagcaga ttcagaccat tgagtaaagt aagtgcccaa
    83221 gaccccatca tcaagtttga ggtattcttc taaagtaaag cttccgtcaa gaaatggcag
    83281 aagtaattga gaatggagtt caaaactgct ggcatcttct tgatacaact cgtgtgcgcg
    83341 atgaagcaga tgctccaaaa ttacttccat accacgggaa acaggatgga agtacacttg
    83401 cacatacatt tgatatcggc taacgatata atcttccact gcatgcatgc cactcatgga
    83461 aaaagcgatc ccgccttcat aaggacgaat cacacgtaat attcgggtaa gatcaaatgt
    83521 tccatattct gttccagtga aataagcatc ccgaagcaaa tagtccattc gatctgcatc
    83581 gatctgacta gaaatcattt ggacaacttg tgggttgggg taagtttttt ggatcacact
    83641 agctaccttt tcaggaaaac cttcttctac tcggttaaga atctgataca cttctgtttc
    83701 tggagaagtg atgatctcta cagtgatggc ttcatgattc gtatggaaga tatgttcaaa
    83761 tgtatgagag taaggaccat gaccgacatc atgcaacagt gccgcacata aagtcaccag
    83821 ccgttcctga tcatcccagc ctccgttgcc tattttttct tttgaaaaat ttcgagaaaa
    83881 gatatcgcag attcgtcttg aaatctcata aacgcctaat gaatgagtaa aacggctatg
    83941 ttctgctccg tgaaaagtga aagaagaagt gcctaattgt ttaatgcgtc gtagacgttg
    84001 gacttctttc gaattgatca aatctaagat tacttgatgc tggacatgga tatagttatg
    84061 gactggatca cgaaaaactt tttccatagg cagcatttga tatttataag gggttgtcat
    84121 aatgtagctc ctttatgtta ttacgttgtt ccattctctg gatttggttg ttccattgct
    84181 ccagctggat ttcttttttt agccagtcgg tagaatcctg taaatcactt gcagaataca
    84241 gcttttcaaa agcttttgca aatgcttctt tgacttcttt tacaaaaagg ggttggtcaa
    84301 gcagttcttc aagtgttgcc attgtagcag gatccactgc aggatagcca tttgtaccaa
    84361 agtcttcttt taaagcagat tgataaaatc gcttcatgac ttgcccgcgt ttttcctgat
    84421 taccgttgac acttaagtac atcataacag aaatcccatt tttgatccga cgctgagcga
    84481 taccggcaaa ttttttgcca tggatgctta aatcataagt tccaggacaa taagaatggg
    84541 tgatttcgaa tgcttgtatc ggtgcctttg gaaaagcgta tttagtcaat aggagcattt
    84601 gctcgtaagc gtcatctatg gttatttttt ttgtgtcact ttgggggaaa atcaaagaaa
    84661 tattcaaaat tccttcatct gatacgatcc ctaatccacc tgaatttctg atcactggag
    84721 cgtaacctgc atctttcagt gtttggatcc cgtcacttag aaaaggagtt cgaacatcct
    84781 tcatacctaa gatgactgtg cagtcatatt gccagaaatg taaaaagatt tgctggtgtt
    84841 ctttagaata ttccgttaat atatctgtta atgcaaaagg ggaaaagtcg tttttttgat
    84901 aaatgtgctg atctagtcga atcgcagggt tcattcaacc ggctcctttg tataaattca
    84961 tattttctac aatattatac tctaaaatca aagagttgtt ttggaaaaaa ggttttctgt
    85021 aaaaatggac aagttggttg acaaaacaaa agaaactagg aacaatgtag ctatgagaag
    85081 taaaggagaa tgaataatgg aattatcaca aggaacaccg atttcaatca aattgcgtac
    85141 aaaagtaaaa cagcaagggg aagtgcaaga tttttatttt gatttgaaag ggcaaatggt
    85201 gacaattggc gatacgctgt acatacgtta taaagagatt caggaggaga cgggcgatga
    85261 gattcctgta acgatcaaat tagttccaga tggtcatgta cagctgatcc gtgcaggaga
    85321 aatgcggatg cgtttgaaat ttggctataa agaacggtta gaaacatcct atcgaacacc
    85381 atacggtatg atccagattg ctacttttac gaaagaactg catgttagtt tgaaagaccg
    85441 tcccacagga gggaaggttc gaatcgatta tgatctgttc atgggaccgg aaaaaatcgg
    85501 cgaatattat ttgaccttgg attttacagc gtaaatataa aagaaaagtt tgattttttg
    85561 tgtctgattt tgtatgataa ggagtagact gtgaaaggac gtgtcctgat tggaaattag
    85621 tgtatttgaa ggtgcaaaca aaagcgaatt atcaatgatc gaagtagcac acgcgatctt
    85681 agaacaacgt ggcgatgtca tggatttttc tgatttagct aaccagatcc aaaactatct
    85741 tgggaaatca gacagtgaaa ttcgtgattc actcgcacaa ttttatactg acttgaatat
    85801 tgacggcagc tttatctcat taggggataa tcgttggggc ttgcgttcat ggtatgctat
    85861 cgattcgatc gatgaagaag taaaccatgg aatggacgaa gaggatgaag atactccacg
    85921 tcgccgtaaa cgcaagaaag tcaacgcctt tatcaatgat gatgaagacg cgatcgatta
    85981 caatgacgat gacccagaag atacagatct gacagaagaa gatgacgatg atctgtttga
    86041 tgacgatgat gatgaagatg aagaaatcgc tgcttataat tctgacttgc aagaaatcgg
    86101 agcagatgac ccgacagatg atgaagaaga cttgccagga aatatcgaag aagatttaag
    86161 tatcatagac gaagacgatg atgatgaaga ttatgaagag gaagaatttt ctgactctga
    86221 aaaagaataa acattatttt cttacgagag gttgtgacag agacgatcag cctatcagaa
    86281 taaggaggtg tgacacgatt tttgtcacac cttctttttt gtattcttaa ttgtcaaatt
    86341 aagttagaca aatcatcaat acttacgtaa ctcaaaaata cttccaaagg tgttcgataa
    86401 ttcagtgatt ttctaggaat attatttcgt ttcgatgcga cagattgaat aaaagattca
    86461 tctactgaat tgaaatccat agatttcaat aaaccatctc tacgtaacaa tccgttagaa
    86521 ttctcgttta ggcctctttg agacggcgtt cctggatcag cgaaataaat ggcaatatca
    86581 ttggcattac tgatctgttt ccaatttgaa aattcctttc cacaatcaaa agtgatggat
    86641 ttgaatagat ttttcggtac agattcaaac caatgattta atttgttttc aatatcaatc
    86701 gcttgtctac cacacggttt caatgtgatg ataacttttg ataatcgctc aactaaggta
    86761 attacagcac ttttatgttt caaacctacg atagtgtcac cttcaaggtg accaaactca
    86821 tttgagaatt ggctataatc tttttcacgt tcatgaatag agcgacggaa agcttgtttt
    86881 ccacgtcttt cttgatgtcc atttggttta cgcttgcctt tcatcggtaa gtcagaagga
    86941 ttaaatagcc cctttttgaa catacgataa atggtacgag cagaacaacg aataggaaac
    87001 gctgcacgac caacaatcac atcgggtgtc catccttgaa caacctttct ttgaatatat
    87061 tctgattgtt cctcaggtaa aacaagcggg cgtctaccac agtttgattt gtttttctta
    87121 tactgttgat agtactctaa ggctgatttt ccttgcttga agaacaggta tactttatgg
    87181 atcgtttgtc ttgaacgatt caagaaatgc gcagttttcg caacagattg atttattttg
    87241 aaataagact ctatcattac aagttcatcc gttgtaagat gggtataggt catttgtact
    87301 cactccttat aattctttgg acggaactat tttgagtgta gcacaaatga cttttttagt
    87361 tgtctagctt aattttacaa tcggcgttat aaagaaattc catctattag ttaagtgaaa
    87421 gtgaaccagt cctcattcta tcacatcttc acttttttga tgatgcaaat ttttattttg
    87481 gtttctatat atgaatctaa atttttgaca gcgtttccta aataagagat gatagacgaa
    87541 caaaacagaa aggagacgaa acaaatgaat agtatggttt ttgttaattt tcctgttgct
    87601 gatgtcaatc gttccgttgc tttttatgaa aagctgggtt ttaaaaaaaa tgaagaattt
    87661 tctactgaag aagctggttc aatggtatgg gatgacactt tttggataat gcttctctct
    87721 catgattttt ataaaaaatt tataaaagat aaagaaatag ctgataataa aaaaacaagt
    87781 ggtgctttga tttcgtttag tgttgaaaat gcggatgaag taaaaaaatt tgctcaaatt
    87841 gcaaaagaaa atggtggtac tttccacgaa gtagatatgg gaatgcctga agaagaaatg
    87901 ttttcattag aagtccaaga tctagacgga aatacattag aacccgtatg gatgaagatg
    87961 taagcagata actaatctga cgttttttat aacaaaaaaa caaaggcgga tgatacgata
    88021 agtattctcc gcctctattt tttttattcg tacaatctct tttttacagt ttataaaagc
    88081 agtcttctca cccttttttt aattgttaga tgccagtgta tgtaaatcac caatgtgtca
    88141 tgacacattg gtgattttat ggtatccttt tattattaac tgacaaagga gagaatattt
    88201 tgaacacttt gattgcaaaa gacgttttta gattgcccga aatgaatgca agattgttgt
    88261 cgacctctgc tcttttatta gcccttgatg tcatcttata caagatagcg attggtcctg
    88321 cttattttca aatcacattc ggatttttaa gtatggcgct tatgggttat ctatatggac
    88381 cgatctgggc tggtttgctt gaaatgttgt cgaacacttt aaatttcacg atatttggtt
    88441 caggaacgtt tcaagctgct tttcttatca cggctttcct tggtggattt attaatggct
    88501 tatttttata tcataaaaag ataaacctgc cttccgtcat cgctgcccaa ttattgatta
    88561 tgatcccagt cagccttttt atcaattctt ggctgatgtc tgttttgttt ggtgcagatt
    88621 tcaaagtaat ttttctaggt agattcatca ggaatatgat ccaaatccct attcaaataa
    88681 tggttgttta ttcatttatc aaaactttag aggctagaaa agtgtttaaa cgagctgttt
    88741 gattttacgt actaaggtag taaactgatt ttggattaaa caataaagcg tgcagtctca
    88801 acagactgca cgctttattg tttctcactg gatccatttt atttcatcaa gtggataata
    88861 gacaaatctt gctttaccta gaatatcagt tttattgatc gtgccaaacg aacgactatc
    88921 ttttgaaact cttcgattat ctccaagtac aaaataatta tcttccggaa gcttggattg
    88981 tcccgttagc tcttgtagtg taaagtccgt cgtataggat gcatttgcat gatctgattt
    89041 caggttttta gttagataag gttccttaac tactttccca ttgatcttca attgatcatt
    89101 ttggtagctg acattttctc ctggaagacc gattactcgt ttgatataaa tcgtgccatc
    89161 tgctaattga aaaacaacaa tatcaaatcg tctgatctcc gaaaattttt ccatcacaac
    89221 catatcacct tgcttcaaca cgttttccat tgaattgcct tccactggta ccgggatgaa
    89281 taaaaatccc cgcaacatga acgcaaggaa tacagaaaca agtaagtatt tgaatacaag
    89341 ccagaagcga ttaatatagc gttgtttttt tgtcaatctc tatcaatcca tccctctttt
    89401 tttcattctt tctttattat aagaaaaata tcaaaaaaat gcgactagat agctgaaata
    89461 aaaataattc tttcaattag gctattggac aaaaaacttt tttgatcata aagtacaaaa
    89521 ctatctctta tatgtcatga ttattttcta aatatttttc ccatgcttcg tcaaagatat
    89581 ccatggacgg caaatagtcg acattcagtt ccagataatt agaaatttct tcgtagctag
    89641 agctgtgctt aggaaattgg atgtctttac cggcttctgt agcgaaaaca gataaagcat
    89701 catgtgtgcc accgcctcgc agtgtcatta aataatgaaa aaagcttcga ttcacccttc
    89761 gttcctccaa ttttcttcga taaaggcagc ccggcgtgca tggtttcttt taaaattttc
    89821 aggatgcttt ttataatact cttgatggtc ttcttctgct agataaaatg gttgggctgg
    89881 ttcgattttt gtaacgattg gctgttcgaa acgtccactt tcagctaact gtttcttact
    89941 tttttcagca atgatccgct gttcttctgt caaataaaag atcaccgggc gataattgtc
    90001 accgcgatcc tcgaattgtc cgaaagcgtc agttggatca gtttgctgcc agtaaagttc
    90061 tactaattgt tcatatgaaa tcaatgttgg atcatagata atctccactg cttctgtatg
    90121 tccagtcgtt tggcttttta cttgttcata agtcggatct ggtacatgcc ctccagtata
    90181 tcctgacatc actgtatgga taccaggaag cgtgtcgaaa ggctggatca tacaccagaa
    90241 gcagcctcct gcaaatattg ctcgttccat tatgattccc cctctaatac tactcatccg
    90301 atgatttttt attttctttt gtcttgcttt cagtactgct ggtgctagtt ttgttggtat
    90361 ttgagttgtt tgaagcatca gaggtatctt ttggcaaata tagactaaac cgaatgtcat
    90421 catttactaa gtcgatttta tctgctttaa tatacatccc gttttgcatt cggaattgat
    90481 ccaacctaag catgatagtc agatcatctg tattgacttc gatccaatct ggtaatttat
    90541 agtttctctt gatcatattc atcacatctt tgattggcaa actcaatgtt ccgatagaaa
    90601 gacttctagc ttttagttgg acatttccgt tttccatcac atacggatca aaatataaat
    90661 agaaatcgat aggaaaacct agtacttcga aagtaccatt gagtagtgct tcattttcca
    90721 aataaaattt atattcaata tcactatctt tttgatactc actaaggaaa aagctgatga
    90781 tttgattgac tttttcttta tttgaattga tcgttaagac aggatctcct tgtctttccg
    90841 taagagctgg tatttctggg agttctggtt cccgattagc aaaaatacgg ctgcctaaaa
    90901 atacggtcga accaagtact accccaagaa ggataaggaa ggcgaatttc cagtaatttg
    90961 ttttctttgt tttgtttgaa gatacgtcag atcttgtttt catgtttatt gatcatcctc
    91021 cttctctaac cattggtttt gtgtctcgat cagctgatct ctcactgcat ttgccatcaa
    91081 ttgataacct aaattatttg gatggaaatg atcttcttca tagagcacat tatttttgat
    91141 gccatttaga ttttctgtcg tgctagttgt ttgatctgtt ccttcttctg tgacacctac
    91201 ttcctgattc aatccttgat aaagcaaatc attgattggg ataaagtagc agttcggcat
    91261 tttttttgtc accgtttctg ttcctttgtt ccaattgtta acaattgtct gcatgtcagt
    91321 gatctcagga aagttcaaat aaaatggatt gtagatgcct aaaatgtaaa taggtgcggt
    91381 tttattgagg gatcgaaatt cttccaatag ttctgtgaca ttttcctgat agtttttcag
    91441 tggcttttta aaggaattga ccgttaaccc gaataaatta ttttggataa ctttcatcaa
    91501 atcattaccg ccaacagtca gtgtgataaa atcagctgtt ttgatattgt ttcgaatttc
    91561 ttcttttgtt tttactcgct ttaagatctg gtcacttcgt tcaccattta caccatagtt
    91621 ttctgtttca accgcggtca aattataacg gtcttttaga tcatttgcga caaggggaac
    91681 gaatcctcct tgcttggttt gatctcctac tccttcagtc agcgaatctc cgatagctat
    91741 gtagtgaagt ttttcttttt tatttggatc ttctctcaca gtagttactg gtttcattag
    91801 tgggccagct ttcggtatag aagcagctaa acctgcatag atgatgatcg caattaataa
    91861 tgggaacaaa acgaggatca aatgtcgttt cgttaatttc attttttcac ctcttgtatt
    91921 cgattggttc taatgttatc aaaaatttta tcaaaaaggt agtctttatg aaaaaagaaa
    91981 acaattattt tcctaatttt ataaagaaaa agagaataag ctgcaagcct ttcaggctga
    92041 gggcttatcc tcttttcgac taatctacat aatacatgat tgcaaacgcg tctttaccag
    92101 tatgcgtagc gataaccgga ttcgtatgca gtacagggat atgcatgtcc gggaataatt
    92161 cttgcaaacc ctctttgaat tcttgcgcca attcttttgc atccgcatgt gaaataccga
    92221 tttgtcgcac atttggcaat tctttcagtt catttttcaa attattaaac catttatcga
    92281 acgttttctt tcctcgcccc ttagttacag ggattaattc agaatgggcg aaattcatca
    92341 ccacgcgtat gttcaaaaca ctagacagca aaccagttgt tcggctgatt cttccacctt
    92401 ttaccaagtt atctagtgtg gatattccga tgtataattt tgtattgtct tgtacttttt
    92461 tgacttcttc aaggattttc tctactgttt taccttcttt tgctaattca gctgctcgaa
    92521 ttacctgaaa agataatcct tggtcagtgg tatcactgtc gataaccgtt actttcgttt
    92581 ttgttagatt actggcttgt cttgctgctt ctactgttcc actcaagcct tttgtcatat
    92641 ggatcgaaat cacttcactg ccgtcttctc ctagctcatc atacatcgag atgaattctc
    92701 caattggtgg ttggcttgtt ttgggcagat tttttgcttt cgccatcatt tccataaatc
    92761 tttcgccgtc tagttggtca tcatcgggat atactgttcc gtcgatcatg atggatagcg
    92821 gcatgacacg aatgcctaaa gcatcgcgta catttttttc catcgtacat gaagaatctg
    92881 ttacgatttt tacatttgtc attattttct ctctctcttt ctttaacaag atgccttatt
    92941 catgttaaac tacatgtaag acaatttcta tttttagtat aacagacaaa taatgatttt
    93001 tcattaatta cacctagtaa aggagccgtt ttcatggaaa aaacgaaatt taatcgaacg
    93061 tatgaaatat taaatgaagt gtttaatgca gtcacccatg gaattgggac tggattgagt
    93121 atcgcgggac tagtggttct tttagtaaaa ggtgcccact tgcattcctc cctccatatt
    93181 gtctcttatg ctatctatgg atcgatgatg attcttttat ttttaacgtc tactttgttt
    93241 cattcattga tttttacaaa agcaaaaaag gtgttccaag tattcgatca tagttcgatt
    93301 tttcttttga ttgcaggaag ttatactcct ttttgtttgc tgagtatccg aggatggcta
    93361 ggttggactt tatttgtatt aatctggttg tttgcgattc ttggcattat atataaagcg
    93421 ttgacgttgc ataaacggga gagagtatca aaagtatcta cgatcatcta tatttttatg
    93481 gggtggctct gtgtcgtggc tgctgttcct ctttatcact ctcttggaat gactggtgtt
    93541 attttgatgg tagcaggcgg gctttcttac acgataggcg cattctttta tagcttgaat
    93601 tctatccgct atatgcatgt tgtttggcat ttgtttgtta tgttaggtgc aggttttatg
    93661 tacttctctg ttttattctt tacttaataa aaaaaataaa gctatggatg ggacagctct
    93721 aaataacagc tagtctatcc atagcttttt tatatcgtca attcgcctgt cccgcttttt
    93781 acaggattct cattacgctg atacgtttca aattgatgcg gataacgatt tttttcatcc
    93841 atttctcctt gactagtatt gatcagtgac caatcagacc agtcaacagg tggaaaataa
    93901 gcatctccat cgaatacatg atggatgact gtacggtata atatcgtaca aaatggcaga
    93961 aattcttgat aaacagctga ccctccagcg atgaaaaaga taccgtctgt ttctttttcc
    94021 agagcaagca cgtcttccac actatgcatg acatgcacgc cttctgcttt ataggaagaa
    94081 tctctcgtca aaacaatcgt ctgacgattc ggcaacggac ggctacccat cccttcgaat
    94141 gtttttcgtc ccatcactag tgtatttgct tctgtcattt gtttgaagaa tttcagatca
    94201 tttggcaaac gccaaggaag acggtcttct tttccaatca atccgttttc atcctgtgcc
    94261 caaattgcag ctaacatttt ctcatccttt ctgattctaa acagcgatag gagctttgat
    94321 cgccggatgt gggtcatatc cttctaattg gatatcttcc atctcaaaat caaatactga
    94381 ctgcttttcc tgattcaaaa ctaaagtcgg aaatgcgcgt atttctcggc tcaattgttc
    94441 tttcatttgt tcgatatgat tgatataaag atgagcatct cctaatgtgt gaacaaattc
    94501 gccaaccgct aaacctgttt catgtgcaat taggtgagtc agcaacgcat aacttgcaat
    94561 gttgaacgga acgcctagaa acacatcccc actgcgttga tacaattggc agcttagccg
    94621 tccctcttga acataaaatt gaaacatcgt atgacaagga ggcaaagcca tgctaggaac
    94681 atcttccgga ttccatgcag aaacgatcaa tcttctagaa tcaggagtct ttttgattgc
    94741 ttcgatcact tgtttgattt gatcgataaa gccaccgtct tttgtttccc agtgacgcca
    94801 ttgatagccg tagatgtgtc caagatcgcc gtatgtttca gcaaaggaat catcggttag
    94861 gatcttctcg caaaaaagct tgctctgctt tttatattct tcattgaatt caggatctgt
    94921 caaacatcta tggccaaaat cagtcatatc tggtccatta tagtctggac ttttgatgta
    94981 gcgttcaaat gcccattcat cccaaatatg gtttttattt tgtaatagat agcggatatt
    95041 cgtatctcct tttaaaaacc aaagcaattc gcttttgatc aatccaaaag gtactcgttt
    95101 cgttgttaac aaaggaaacc ctttttgcag atcaaaccgc atttggtatc cgaataagct
    95161 taatgtgcct gtgcctgttc tgtcttcttt acgatgccct tcttccaaca attttcttcc
    95221 taagtcaaga tacgcttgtt ccatcctttt tcctcctaat cttctaccca ttcactcaag
    95281 tattcccacc gtgccatttt ttcttcaagt tgtgcttctg tttcagatac gtcgttttgc
    95341 aattcttgta atcgagtgaa attatctcct tgatgattca tctcttcttg gagcaatgag
    95401 attttttctt caagttctgc aatctcatct tcaatcgttt cccattcttt ttgttccatg
    95461 taagataatt tcttcttttc agcaggttcc ttgctctgta tttttggttt cgctttttca
    95521 gcaggctgct gggtctcttt ttgcttcagt aaataactgg acatgtttcc gtaataaagt
    95581 tcttgttttc cgtttccttg gaatactaat aacttatctg ctaccttgtc caagaaataa
    95641 cggtcatggg aaacagtgat cacagcacct ggaaaacttc taaaataatc ttccaaaatc
    95701 gtcaatgttg ctatgtccaa atcattggtc ggttcatcca ataataaaac attaggttgc
    95761 tgaatcagca gtttcaacaa aaataaccgg cgtttttctc cgcctgaaag tttgcctatc
    95821 agtgtcccat gcataaatct tggaaataaa aatcgttcaa gtaattctgc tactccgatt
    95881 tgactgccat ctgctgtttt tgcttcttca gcagcttctt gtaagtaagc aatcatccgt
    95941 ttatctggtg ccatttcttc attttcttgt gtataatacg caagatgaac cgtttcaccg
    96001 atagacattg taccgctatc caggggaatt ctgccagcta gaatattaag caatgtcgat
    96061 tttcccgcac catttttacc agtaatacct agtctttccc gagattgaat caataaatct
    96121 aaatgttcta gcagtgtttg gtcatttata cggtaactgc catctttgat ttccagtact
    96181 tttttcccta aacgttgtgt cgctaggttc agctctaaat catcttcttg gtttacttgg
    96241 tacaagttct cctttagatc ttcgaaacgg ttgatccgtg cttgctgctt ggtgccacgt
    96301 gcctgtacgc cagccctcat ccaagctaat tcctgtttat aaagccgctt gcgcttttct
    96361 tcttgttcta cttctacacg atcacgttct gctttttcta agacgtaagc ttcataattt
    96421 cctttatact cgtataattt tccaaatgaa agttcgaaga ttcggtttgc tactctatct
    96481 agaaaataac gatcgtgggt aaccatcaat agtgcgccac ggtattgttt caaatagttt
    96541 tccaaccact cgatcgcttc ataatctaaa tggtttgttg gttcgtctag taatagtaaa
    96601 tcgggttcat caatcaacac ttgcgccaga ctaactcgtt ttttctgacc acctgataaa
    96661 gtactgattt ttttatctaa ttcactgatt cctaattttt gcaaaataat ctttgcgttg
    96721 gtatctgtag tccaagcatc ttctttattc atttgttctt ctgctttagc ataacgtttt
    96781 tgcacatcag ggtcttctcc gttttcccct agttcgatca atgcttcctc atatgctcga
    96841 acagtttgga tcaaggggct gtttccttga aagactgctt ggagaacagt taattcatcg
    96901 gaaaattcag ctgcttgaga aagataacca atacggtaat cagttggata aaacactgtt
    96961 tgccgatcac cgtcgctgct atcgataccg gcaataatat ttaagagact tgttttaccc
    97021 gtcccgttcg tcccaatcaa cccgatacgg tctttttcgt ggaccaaaaa agaaatctgg
    97081 tcaaacaaat ctttttcacc ataagttttt ttgaggtcag ttacttttaa ttctttcaac
    97141 atgttccctc cttatcttgg atattgtatc ataaaaatag tgaacttcag gaaaattttt
    97201 ttggtatttg ttctttttgt gttctcaaaa aaagaacag
//
LOCUS       DACESX010000002        68125 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.002, whole genome shotgun sequence.
ACCESSION   DACESX010000002 DACESX010000000
VERSION     DACESX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.002"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(160..2346)
                     /locus_tag="IXF88_000101"
     CDS             complement(160..2346)
                     /locus_tag="IXF88_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1506862.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2564..3247
                     /locus_tag="IXF88_000102"
     CDS             2564..3247
                     /locus_tag="IXF88_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1506863.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3328..4047)
                     /locus_tag="IXF88_000103"
     CDS             complement(3328..4047)
                     /locus_tag="IXF88_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1506864.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4359..4565)
                     /locus_tag="IXF88_000104"
     CDS             complement(4359..4565)
                     /locus_tag="IXF88_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506865.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4810..5412
                     /locus_tag="IXF88_000105"
     CDS             4810..5412
                     /locus_tag="IXF88_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1506866.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5553..6833
                     /locus_tag="IXF88_000106"
     CDS             5553..6833
                     /locus_tag="IXF88_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1506867.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6846..7574
                     /locus_tag="IXF88_000107"
     CDS             6846..7574
                     /locus_tag="IXF88_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1506868.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7643..9166)
                     /locus_tag="IXF88_000108"
     CDS             complement(7643..9166)
                     /locus_tag="IXF88_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1506869.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9418..10599
                     /locus_tag="IXF88_000109"
     CDS             9418..10599
                     /locus_tag="IXF88_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1506870.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10659..12287)
                     /gene="treC"
                     /locus_tag="IXF88_000110"
     CDS             complement(10659..12287)
                     /gene="treC"
                     /locus_tag="IXF88_000110"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1506871.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGVKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12738..15449)
                     /locus_tag="IXF88_000111"
     CDS             complement(12738..15449)
                     /locus_tag="IXF88_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1506872.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15459..16160)
                     /locus_tag="IXF88_000112"
     CDS             complement(15459..16160)
                     /locus_tag="IXF88_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1506873.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16346..16945
                     /locus_tag="IXF88_000113"
     CDS             16346..16945
                     /locus_tag="IXF88_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1506874.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17026..18039
                     /locus_tag="IXF88_000114"
     CDS             17026..18039
                     /locus_tag="IXF88_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1506875.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18147..19064
                     /gene="rbsK"
                     /locus_tag="IXF88_000115"
     CDS             18147..19064
                     /gene="rbsK"
                     /locus_tag="IXF88_000115"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1506876.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19092..19487
                     /gene="rbsD"
                     /locus_tag="IXF88_000116"
     CDS             19092..19487
                     /gene="rbsD"
                     /locus_tag="IXF88_000116"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1506877.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19487..20860
                     /locus_tag="IXF88_000117"
     CDS             19487..20860
                     /locus_tag="IXF88_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1506878.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21217..23361
                     /locus_tag="IXF88_000118"
     CDS             21217..23361
                     /locus_tag="IXF88_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1506879.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFNHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23413..24453)
                     /locus_tag="IXF88_000119"
     CDS             complement(23413..24453)
                     /locus_tag="IXF88_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1506880.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAHSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24730..26532)
                     /locus_tag="IXF88_000120"
     CDS             complement(24730..26532)
                     /locus_tag="IXF88_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1506881.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26546..27298)
                     /locus_tag="IXF88_000121"
     CDS             complement(26546..27298)
                     /locus_tag="IXF88_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1506882.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27307..27669)
                     /locus_tag="IXF88_000122"
     CDS             complement(27307..27669)
                     /locus_tag="IXF88_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1506883.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27845..28717)
                     /locus_tag="IXF88_000123"
     CDS             complement(27845..28717)
                     /locus_tag="IXF88_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1506884.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28768..29256)
                     /locus_tag="IXF88_000124"
     CDS             complement(28768..29256)
                     /locus_tag="IXF88_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1506885.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29394..30224)
                     /locus_tag="IXF88_000125"
     CDS             complement(29394..30224)
                     /locus_tag="IXF88_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1506886.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(30240..30848)
                     /locus_tag="IXF88_000126"
     CDS             complement(30240..30848)
                     /locus_tag="IXF88_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1506887.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30841..31158)
                     /locus_tag="IXF88_000127"
     CDS             complement(30841..31158)
                     /locus_tag="IXF88_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1506888.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31314..32042)
                     /locus_tag="IXF88_000128"
     CDS             complement(31314..32042)
                     /locus_tag="IXF88_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1506889.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32201..33082
                     /locus_tag="IXF88_000129"
     CDS             32201..33082
                     /locus_tag="IXF88_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1506890.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33319..34362)
                     /locus_tag="IXF88_000130"
     CDS             complement(33319..34362)
                     /locus_tag="IXF88_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1506891.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34365..35033)
                     /locus_tag="IXF88_000131"
     CDS             complement(34365..35033)
                     /locus_tag="IXF88_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1506892.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35175..37511
                     /locus_tag="IXF88_000132"
     CDS             35175..37511
                     /locus_tag="IXF88_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1506893.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37626..37937)
                     /locus_tag="IXF88_000133"
     CDS             complement(37626..37937)
                     /locus_tag="IXF88_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506894.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38118..39593
                     /locus_tag="IXF88_000134"
     CDS             38118..39593
                     /locus_tag="IXF88_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1506895.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39640..40224
                     /locus_tag="IXF88_000135"
     CDS             39640..40224
                     /locus_tag="IXF88_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1506896.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40226..40645
                     /locus_tag="IXF88_000136"
     CDS             40226..40645
                     /locus_tag="IXF88_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1506897.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40926..41210)
                     /locus_tag="IXF88_000137"
     CDS             complement(40926..41210)
                     /locus_tag="IXF88_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ1506898.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41562..42272)
                     /locus_tag="IXF88_000138"
     CDS             complement(41562..42272)
                     /locus_tag="IXF88_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1506899.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42269..43741)
                     /locus_tag="IXF88_000139"
     CDS             complement(42269..43741)
                     /locus_tag="IXF88_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1506900.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43879..44352
                     /locus_tag="IXF88_000140"
     CDS             43879..44352
                     /locus_tag="IXF88_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1506901.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44505..45056)
                     /locus_tag="IXF88_000141"
     CDS             complement(44505..45056)
                     /locus_tag="IXF88_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1506902.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45161..45691
                     /locus_tag="IXF88_000142"
     CDS             45161..45691
                     /locus_tag="IXF88_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1506903.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46174..46617)
                     /locus_tag="IXF88_000143"
     CDS             complement(46174..46617)
                     /locus_tag="IXF88_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1506904.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46633..47793)
                     /locus_tag="IXF88_000144"
     CDS             complement(46633..47793)
                     /locus_tag="IXF88_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1506905.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48300..50000
                     /locus_tag="IXF88_000145"
     CDS             48300..50000
                     /locus_tag="IXF88_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506906.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50102..50935)
                     /locus_tag="IXF88_000146"
     CDS             complement(50102..50935)
                     /locus_tag="IXF88_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1506907.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51239..53404)
                     /locus_tag="IXF88_000147"
     CDS             complement(51239..53404)
                     /locus_tag="IXF88_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1506908.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTCFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53664..55958
                     /locus_tag="IXF88_000148"
     CDS             53664..55958
                     /locus_tag="IXF88_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1506909.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55951..56616
                     /gene="pgmB"
                     /locus_tag="IXF88_000149"
     CDS             55951..56616
                     /gene="pgmB"
                     /locus_tag="IXF88_000149"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1506910.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56609..57616
                     /locus_tag="IXF88_000150"
     CDS             56609..57616
                     /locus_tag="IXF88_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1506911.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57638..58657
                     /locus_tag="IXF88_000151"
     CDS             57638..58657
                     /locus_tag="IXF88_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1506912.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58938..60305)
                     /gene="nhaC"
                     /locus_tag="IXF88_000152"
     CDS             complement(58938..60305)
                     /gene="nhaC"
                     /locus_tag="IXF88_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1506913.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60414..61835)
                     /locus_tag="IXF88_000153"
     CDS             complement(60414..61835)
                     /locus_tag="IXF88_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1506914.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(62078..63955)
                     /gene="tdc"
                     /locus_tag="IXF88_000154"
     CDS             complement(62078..63955)
                     /gene="tdc"
                     /locus_tag="IXF88_000154"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1506915.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64246..65502)
                     /locus_tag="IXF88_000155"
     CDS             complement(64246..65502)
                     /locus_tag="IXF88_000155"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1506916.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            66081..67382
                     /locus_tag="IXF88_000156"
     CDS             66081..67382
                     /locus_tag="IXF88_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1506917.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKTLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67451..68050)
                     /locus_tag="IXF88_000157"
     CDS             complement(67451..68050)
                     /locus_tag="IXF88_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1506918.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        20435 a        13813 c        12122 g        21755 t
ORIGIN      
        1 gacaaaattc ttgtcacacc tcccttttcc gaataaactg tgttccagaa atagcttctt
       61 cgaaaataag ctgaaatttc acaaaaattt gagaagcaat tttcgtacat tccttcttct
      121 ttcttgaaac tgaccacttc tgtcacaccc tctcttctat cactttcctt ttttcacacg
      181 actcccggtt tctttgataa atctcgatac tttcaatttt cttgaatttt tcatttgagg
      241 tacacataga tacagacgat ttcttgctct agtcatcgca acataaaata gtcttcgttc
      301 ttcttctatc gtcgttactg gttctgcttt tatttcatta aagtcgatta ctttcttttc
      361 tgtcgctccg ttataaggca aacttccttc gttacaacca atcaaaaaga catttcggta
      421 actaagacct ttacttttgt gaatgctctg gatacatagt gcatcaagtt ttccttgtct
      481 gtaagcaatc aagcgggttt ttaattcttt agctgtcgct tgggtttctt gcgataaggc
      541 ttggagtctt ctgaaagtag catgctgctt tacttcttct cgtatccatt cttgatcact
      601 ctggtcgatt tccttagcta tacgtggcag atgaagatag ctttgaacaa gcgaatagat
      661 ttcttgatgc tggtctgttt tacgcaacgc ttcctcaaac tctcggattt ctgcagattt
      721 cgattttaaa ttggggctta tcgacaaaat ataatctgca acggatatat tttgatattt
      781 aggagaaaag tattgatcgc attcactttt tttcaggtag cagtctgcca tcagctgccg
      841 gagcaattgc tggtaggcct gatttttctt ttgcagattc agtgctgttt gccaatgaac
      901 aattttttga tagaaatgtt ggaaagactt gataccaaag ataccttcat atggactatc
      961 tgcttccagt acaaaaggaa tattcgtttt cagcagagat tggacaagct gctgcgtttg
     1021 tgaagctgat cggtataata tgattgattc cttatatttc ccattttttt gaataaatgt
     1081 ctgaatttgt ttgattagcc actgactttc tgctttagta tcagaaaaca aataggcttc
     1141 cataacacct gcttcttttt gtgctggaat catcgccttt ggtatccgat catttttatt
     1201 atagctgatc aatctatttg ctccttggac aatagctgcc cgagaacgat aattagttaa
     1261 taagaaaaga gttttggtag gatattcttt ttcaaagttc ttgatgtatg ctggctctga
     1321 tcctctgaat ccatagatac tctgatcatc atctccaaca acaatcaact tgatcctgtt
     1381 ttcctttgtc atccgtttca cgatttccca ttgaagtgga ttagtatctt gaaattcatc
     1441 aatgaataga taatcatatt tttggccgat ataatgctgg atctcctcat tctccaaagc
     1501 gcttttcaga ttttggagta catcattgaa ctctagcaat tgctggcttc ttttttgttc
     1561 ttggagttcc gtgagcaatc catacagtcg ttctgatatc ggctgtcgtg cattcttatc
     1621 aatggaatca tatttttctt taactaactg aaccatttct tctactgaaa ggttcatatt
     1681 ctgccaatag tctaaccgag aagcaatcgt tttttctcca tattgatcaa atatataaag
     1741 attatattta cgtatcagct gttgcaaaac agcattcact ttttgtgcag tatcctttga
     1801 aaagtctcct tgtattcctt gcattgctac ttcaggaaat tttcgacaaa tttcattgta
     1861 tatcctaaaa aagatactat ggaatgttcc gatatggaca gcggtttttc tctggctgaa
     1921 attatctact cgttttttta gttcatttgc agcgttcctt gtaaaagtga tcattagtat
     1981 gcgttcttcg tctacttgtt tttgcgtcat cagataaaca gcttttgcac agattgtttg
     2041 cgttttgccg ctcccagctc ctgctatgat tcgaagatgt ttcttcatat catatgtgat
     2101 agctgctttt tgttcttgat tgaagcgatt ataggaacgt ttctccatca ttttaaagaa
     2161 agagctgttt ccttcacttt cttttacttg tacttcttcc tcaataagtc cgggtgcttc
     2221 gccataagcc gcttcatagg tagcaatgat ttcttcttgt agccgaaaga cttgttgaag
     2281 ctttttatac ttgttctccc agcgtttcaa ttctttttgt aatcggagaa tttctttttc
     2341 attcaactga ttgatttcct tcccatcttt cttctttgaa tttgtttatt tcaagttggt
     2401 tatttaaagt ttcgttgtct attttttctt tatactaaca gacctttcta aaaatgaaaa
     2461 tgggaagcga tatgatttta gttttctgaa aatttttcct ttttttcaat cgtttatgct
     2521 aagataagga agacaaaaat attcataaaa ggtgatgaaa aaaatgcaga cttcctttca
     2581 cgaggggcgt acaataggac ttttgctgac atttatcgga ggagccatgg acagttatac
     2641 ttatatccaa tatggtgctt ttgcctctgc tcaaactggc aatatcattc tatcgatcat
     2701 ccaagcattt gacggagaat ggctaagtgt tggtaaaaaa gtattgtcta cccttttctt
     2761 tttcttaggg attcttctaa caaagtatct aattgattat tttagaaaaa aagaaaaaca
     2821 tttctggcgc ttgtttgttc tatattatga agcagtgatt tttttctttg ttagtttgac
     2881 acccgtccat gagcatcccg cattagtaac gattttgatt gcttttactg catcgatcca
     2941 gtgggtagct tttgataaaa tcaacgggcg tgcctacact aatttattta caactggaaa
     3001 tttaaaaggg gtagcaacga acttatatga ttattgtatt tctaaagaaa aagcagattt
     3061 tgatcgattt ttccactatc taagtgtcgt tcttgccttt atcgcaggag ctattgtcct
     3121 tgttttctgt catcatctgt ttggcgccca ttcgattttg atcgtttcca ttttattttt
     3181 gatcatggcc ctcactcaga catttcattt atggcaattt tatcatacga atcggttcac
     3241 tatttgatct caatgaatca aaaaaactaa gcaagatgct gctcacagag aaatatctgc
     3301 gagtgagcaa tttgcttagt ttttatatta tttagttgta aagattccct ttgctttcgg
     3361 gttatttgta gtgtagcgga tcgactgggc atctgcctct tctgtcattt tctttgcccg
     3421 catcttgatt tcttgtacga tttgttctcc acatgccgga tcggctacga aatcatattt
     3481 gtccccatcg atcactattt tgggacatat atcaaactgt tcataccact cttcgtatcg
     3541 tcgattgagt tctttatagt aggaatagag ttcaggatct tgttctagct gttcatattc
     3601 ccggcctctt tttttgattc tcgctaacat agtttcaaag gatacacgaa tataaaccag
     3661 tagatctggt ttcttttgtg gcgaaatttc ttctaactcc ttcagcatcg tttctaaaag
     3721 accttcatac tgctttactt ctacttcaga aacacgccct aaatctgcat tcaaatgaaa
     3781 cagcatacta tcttcgtaga tggagcggtc tagtacgttg tcatcatggc tgaaagcatc
     3841 tttgattgcc aaaaagcgtt tgttcaaaaa gaaaatctgt aataaaaaag tatacttttc
     3901 tggattagaa taaaatagcg gcaatacttc attatcttcc acgttctcat aaaaaggttt
     3961 tgtttctaaa tcttgggcaa gcatttcagt tagtgtgctt ttccctgcac cgatcgttcc
     4021 tgcaacaatg atcacaatta tcggcatcct tcctgtaata aaatctgttc ttcattttac
     4081 tacttcattt atagaaataa aagccttgac tttatctgct ccttaaaagc tacttctcta
     4141 aaagtagcca aaagtaactc ctattcatga aaataaaggc tgtgacaaaa gtcttgtcgc
     4201 agcccttttt tcaaataaac agtattctcc tgccaacacc tcggtcacaa ggcaatattc
     4261 ataaaacatg aaaagctgtt ttttaacatt attccttgta actcagagct aaacgggaga
     4321 ctcacaacct ctttatgctc tcaacatttt atctgttttt attcttctat tgtacttgat
     4381 ttgcgtacaa ctaactgcca gtgcagatta tgagcggtat cattgtcttc tactgcgcat
     4441 ttcatgacac gtatcatcac actattttca tagatttttt ctatttttcc ttttattggt
     4501 gattcaaata aatcagattt aaatgcatat gtttcgccga cttcaaacaa ttgttcattt
     4561 tccataagaa tccctccatc accttttata aggtcagtaa ttttttaaca taaatcactc
     4621 gaaaaagcac aaacttgtca taaaaagtac ttttcgtatc aaaactgtca ctttttttgg
     4681 taaggttctt ggaaaacgaa gattttttta ggagatcatg acggttttga tgtcaaaacc
     4741 actttttatg aaacaaaaga cgttttttat tttttttagc ataaaatttg tggtggaggt
     4801 acgtatgaga tgaaagataa agttgtgaac aaattattag aagccttatt cagcggagac
     4861 aaatcggaag aagatatggt gtatgtccaa gtaaaatttg tcttagaagt tttattgaat
     4921 aatcttggaa aactagcggt cgttctagtc ttttctctgg tgacaggctc atgggcggaa
     4981 acaggaatca cctttctttc ttatatctgc atccgcagat atgcttatgg attgcattcc
     5041 gactctgaat tcgtttgtct gctgtggaca ctactttatt tatggggcgt tcccttggtg
     5101 atgaaacatc tgcaattgac tatctccttc ccaatgatgg tctttttgtt aatcagctgc
     5161 tttttgttac tgcttcgcta tggatcaaga ggtacagcaa tcaatcccat tgaacccgag
     5221 aaacgtcccc cattattaaa gaaggcgatc agcatgtttc taattttttc tctcattacg
     5281 ttatttttct cagcttctta cttttctacc tatttgctac ttgggattgt tttagaaata
     5341 gctacgttgc ttcctattac aaactacttg atgaattttg gaggaatctt tcatgaaaaa
     5401 aacaatcatt aaacaattaa ctaacttatc tgactttttt gtatctggct gctgctttgg
     5461 tatgagttct gaattcaaaa tccctgaaaa attaaaaaaa taatacgcca attgtgctat
     5521 aatttggtaa aaatcaatgg gagctgtact ttatgttttt atggtccata gcgttgcaat
     5581 atttgattta cacaatttgt atcttattga ttgaccatca catccgatac aaatatctgt
     5641 ttgcgggagt cattctgatt tcttgtattt tgattggatg ggtctattcg attttaggga
     5701 tttttactgc tctaattgct ataggaatgc tgatgcttac ttttttcttc ttttcgactt
     5761 cgcgtcaaca attggtttac gcgcttcctt ttgggttatt gacgtcgctt ttaggagatc
     5821 acttaacaag catcggcgat ttttttgttt tgaaatcttc ggttattgaa ccaggagatt
     5881 cattgcagta ttttcacatt gtcttatccg cttttttatc tgctttatta gctttttgtt
     5941 taaaaaaatc gttagagact tggtttcaca ctgtggaccg ccgtatgatc ggtacgatcg
     6001 ggacattgat attatttaca tactatatca ttatttttta tacccgtttt tctggtgaaa
     6061 cacctgatgt cttagctttg aatacgagtt ttttcattct atatctgtgt gttggcctga
     6121 tcatcttggt ttattattgg aaaatctcta ataagaaatt agctgatcag ttattagagc
     6181 aacaagtcag gatgcaacag gattatatca aagatctaga aaaaaactat caagacttga
     6241 gagaattcaa acatgattac caaaatctgc ttttttctct tcacagctat atttcagaag
     6301 gcgacttaga aggattacgg tcttattatg atcaaacgat attacctaca cgccagatga
     6361 tgaacctttt tccggccaat ctaagtatgt tggataatat gaaagttcct gagatccgta
     6421 gtttactgtc attaaaactc atgatggcac aggaaaaagg gttgatcgtc cagcttcatt
     6481 ttcctgagcc aatcgaactg gatacgaaac atacggtaaa ccttgtacgt atgcttggta
     6541 ttatcttgga caatgcgatt gaaggagcta gtgctgcaaa gaagaaaaaa atcgaattga
     6601 ccatactcaa gaagaatcat tccgtgatga ttcgtgtgat caatacaaca acaaatacat
     6661 taccgttaaa ccagttgaaa caaaaagggt tctcaacaaa aacaaatcca aaaaacgaag
     6721 gccttggctt gtttatcttg gatgaattga caaagaccaa tccttatctt ttccttgaaa
     6781 catcaattga aaaccagcgt ttttctcaaa cgatttatat acaaataaaa taaaggagta
     6841 atgatatgat tcccatttat atttgtgaag acgatccaaa acaattagaa gttttatcaa
     6901 cggtgatcaa aaatagaatc atgattgaaa acttagacag ttatgtccat atttctacct
     6961 ctgatccgga agaacttctc aatgcagcca agatcagaac ttcttctttt ggtatttact
     7021 ttttagatat cgaactagaa gacagtgaaa tggatggtgt agcgatcggt aaattgatcc
     7081 gtgaattaga tcctttagga aaaataatct atgtgacttc ccatacggaa atgtcgatga
     7141 aaatcttaaa aagcaacatc gaaccgactg attatatcgt caaagaagat ctttatgatt
     7201 taaaagaaaa tgtcgagcat atcctgagca aaattttcga agattttcaa atgtcccaaa
     7261 tccaaaaaga tatcttcaaa atagagttta atgatgagat caaattttta ccgatcaaag
     7321 acatccaata tttttctact tcgctaggag cgccacataa actggaagtc catttgactc
     7381 atgcccaatt acaattctat gagaagatca aagaaatcga gaaaatgcat cggaatttta
     7441 tccgttgtca taaatcctat gttatcaata cacaaaatgt gcggtcgatt aataaaaaaa
     7501 ccagagaagt cacgttggca aacggcgaga ccatcccagc ctctatccgt ggattaaaga
     7561 aattgatttc ataaaaagaa ccgaatagtt tggaccgact aaatgaccag ccgatccaaa
     7621 cccttcggtt cttttttgcc tatcagtgca aaagcatttc ctgtgcaatt tcacgtccat
     7681 ccattgtatc tggataaaat gttggccaat tctccatttc tttcagcaat acttcttgac
     7741 tttcatttcc tgcatataaa tgaaaatgtt cggagtcagc cggtgcaatc gtgtgatcac
     7801 tgaattgaat atatctaaaa gcgccacttt ccttatcttc tgcttcgaat agatacctta
     7861 cgccacggtt tcctgcctcg taggtcaaga tttctttccc tacataacga taatttgaac
     7921 gtttcgttgt tccattctta aacgtaaatg ccatctcttt ctcactgatc ttgattgaag
     7981 caatatctgt ttgatagccc ttcgtataat attctttata ttcttgtgct gtcttatcct
     8041 gattcaattt ttctttatat tcaaaaacag gatcaaaagt cccatcttct acatacgtat
     8101 ctactgtttg ccaagttcct gcccaatctg aaagagagcg atcttgtaca tcctcgtctt
     8161 caaaataacc gttgtagacg ctcttttctt ttttgggggt caaagtatct tctagcgggt
     8221 tttctgtctc tgtcgttttt ttcaacgctt cgaggtttgc ttccatgata gaaagatagt
     8281 tttctccatt ttctatttgt tcattcgtca atccttccaa aggattcaat acttctaaag
     8341 atacacctgc ttcgtttgct aacgttctgg caatactatc tttggcgttt tcttcaaaat
     8401 aaatatagtt gattccatgt tctttaacaa agtgtttcag ttcagcaatt ctcgccgcag
     8461 acggttcttc actagaagaa agaccagcaa tcgatacttg attcaatcca tattctaacg
     8521 ctagatagtt gaatgcagca tgttgcgtga caaaagatct ttgttttgct tgcttaaagg
     8581 tttcttgata aagctgatgg agtgcttgaa gtttctcggt ataggctgct gcattttttg
     8641 tccatacttc ctgtttttct gggtaagctt caatcagctg atcacgtatg tttgctacct
     8701 gcttgattgc taaacttgga gctagccata catgtgggtc tagctcatgt gtatggcctt
     8761 cttccccgtg ttcatggtca tgatcatgtc cttcttcact tcccggaagt aaaagcatac
     8821 cttctgtcgc ttttatcgta tggacattgc cttcttgtaa tgttttttgg attgcgggga
     8881 cccatgtttc catgttttca tcattataaa caaagatatc tgtatcttgg atctttgcca
     8941 tgtctttagc agaaggttca taatcgtggg aatcagtccc tgctggaatc aataactcta
     9001 cttctccttc atccccgacg attgcttttg taaaatcata catgggataa aaggtcgtca
     9061 tcacgctcaa tttttcttta ctgtcctcgg ctttcccact tgccccgcaa gcagttaaca
     9121 tacctgctaa aaccagcatg gccaatatac ctatcaatcg tttcatttat gacactcctc
     9181 ttctcatctt ctttcatttg aatgttccga ttcgcttttc gcaaaccgta atagttacgt
     9241 tttagcaaca ggatttaatt tatcatatgg ttaaaagaac tgtcaagcgt tttcttattt
     9301 taattgatga gaaagtttat atgaatgatt gacgatattt ttgcaatcaa cctctatcat
     9361 tttcctttta tacccgcttt tgtctataat gaagttatag ataaaggagg aaaaagagtg
     9421 aaaaaagaaa cagatacaac gaatcgtttg atccgctttt taggaggaaa aacctcttac
     9481 tatgttttag gaatagttat cttacttgcg ctgatcattt attttttcca tcagatctcg
     9541 tttatattca agcctgtcgg aatcattatc gctactatct tgcccccatt attgtttgca
     9601 ctgatcttgt attatctttt ggaaccagtc atcaaacgtt tgagcaagca cctttcgagg
     9661 aacttgtcag tcattatcgt ttatgtgatt attttatttt tattggggct tggaagcata
     9721 tggcttattc cttttcttga agaacaggca aagacattga tcagtagttt gccagatttg
     9781 tttcaagatt ttcaagagag tctccgacaa tttttggaga aaacaccttt tgccgattca
     9841 tttaatcaat ttttcgcttc tattgacgac gtgacgtctg atatagctgg atttattgga
     9901 aattattggg aaagtggcgc acaacggatc agcaacattt tttcaactgt tacagctatt
     9961 tttattactt tatttactgg tccaattgtc gcttttttcc ttttgagaga tcctcaaaaa
    10021 ttttatcagt cagttttagc gattgtccca ccagcatttc gtacagattt taaaaatttg
    10081 acaaagattg ctaatcaaca attgggttca tttctaaagg ggcaaatcat tgcttcattt
    10141 atccttggag cagtctactg ggtttgtttc ttactgatcg gattagaatt tgctagtgtt
    10201 ttagctattt ctgctgggtt gttatgtatc attccatata ttgggccgtt tatcgccttc
    10261 tttccaggtt tgatcattgc attccaagac tctactttta tggctgtcaa atttgtcatt
    10321 gtctggtttg ccgtacaact tctccacggt gacttggtga ttcctcgagt aatgggtgat
    10381 cgtttacaga tccatccgat tacgatctta atcgttttac ttgtcatggg tgatctaatg
    10441 ggaattgtgg gcgttatttt tggtattcct atctatacgt tagtcaaatt actcgttact
    10501 tttattttcc gtaaattcaa acaacgctac aataaatttt atggcgataa aggagagtat
    10561 gaaaactctt cattttctga agaagattat tttaaataaa acacaaaacg tcgatcttac
    10621 gagaatagct cgtaggaacg acgttttgtg tttttgtttt aatttttcag caaagcaatt
    10681 gcctggtatg gttttagctc cagactttga tgaagaacag tatcggaata attatcgata
    10741 agaatattgg cttctgtgaa tccttctggg atggcgatcc ttgtatcttt atcatagaaa
    10801 tttgttaata caagcagctg ctgtccttca aattggcgaa gaaagccata aacttccgga
    10861 tgatttgctt cataagcatg ataatctcct tgtgcaacga ttggatatgt ttttctcagc
    10921 cgaatcaatt tcttgtaata ggacaaaata gaatcttcag aagatagttc tttttctaca
    10981 ttaatctctt ctagcttgac tccaactttt aaccaaggtt tccctgtagt aaatcctgct
    11041 tgttctgtgt ctgaccattg catgggtgtt cgagagttat cgcgagattt tgctttgatc
    11101 cgacgaaacg cttctttttc tgtgaatcct tgttcaagga gtaacttata agcattcaaa
    11161 ctttctacgt caatatagtc ggaaatcgat gaatagtctg ggtcgatcat gccgatttct
    11221 tctcccatat aaatataggg cgtcccacgg ttcaaatgga tcatggttgc tagcatctct
    11281 gctccttgtt ttcgatactg atcgatagct acaaatcgat tgagcgcacg tggctgatca
    11341 tgattattaa aaaatagagc attccagccg ttaccctcac tcatcttttc tccccatgta
    11401 tgaaatagtc gctttaattc tgcaaaatca aatggaggag tagaccattt attcccattt
    11461 tcataatcga ctttcaaatg atggaaatta aagaccattg ataattcttc tctactaggt
    11521 tctgtataca agatacaatt ttcgatcgtc gtagagctca tttcaccgac tgtgatgata
    11581 gtggaatccg tcccaaacgt tgcttggttc aattctttta gatacgtatg ggtgattggt
    11641 ttgtccgtat attcaggttt gccatcattt tctttattat ttttcaattg ttcgtcttta
    11701 ccgataacat tgataacatc gaaacggaat ccttttaccc ctttttccat ccaaaaacga
    11761 acaactttga atagttcctc tcttactgct tggttccgcc agttcaagtc agcttgtgtc
    11821 cgatcatata agtgcaaata atatttcccc gtatctccaa atggcgacca agcacttcca
    11881 ccaaattttg atacccaatc cgttggctct tctcttaaaa tgaagaagtc ctgataatgc
    11941 tggtcccctg ctagtgcttt tttgaaccat tcatgttctg tcgaaacgtg attcagcacc
    12001 atatcgagca tgatttctat ccggtatttc tttgcttcgg ctattagctg ttcgaattcc
    12061 tccatcgtac caaagatagg atcgatggct gtgtaatcag agatatcata accgttgtct
    12121 ttttgagggc ttggataaat agggtttagc caaatcatgt cgatgcctag atcattcaga
    12181 tacgggagtt tttctttgat tccttttaaa tctccaattc catcaccatt actgtctttg
    12241 taagatttgg gatagatttg gtaaatgact ttgtctttaa atgtcatgga tatttacctc
    12301 cattcaactg atttattacc taccacaact tatatataca agttatcatt tattattata
    12361 actgaaccga aagcgtttgt aaaatttatc tttatacatt ctaagaggtc tgttgcccaa
    12421 taaaaaacaa gagatcagac atgtatttgt ctatcctctt gtttaaaata gcaaaatatt
    12481 cttgtttctt ctataaatgg tgttcaaaag cttttatcta cggcaatcag tcttctcaaa
    12541 tgtaaaacat gaggagctaa ttttatttga gtattctttt tgctcgatgc aggacggctt
    12601 ttttacaacc tctgattcat ggtgttcaat cagctgtcct tcggtattag ctcaaaaaac
    12661 ggcaaaatat gaaaaactat tttgccgttt gtttctctaa tactcaggtc aaaccgctga
    12721 tttcacaacc tctgttttta ttcattcgat ttcaacgcat caatcatatc gatatgcttt
    12781 aatttgaaat gggtaacaat catcacaata accgtaaata cgatagttaa tcctgctgat
    12841 aagacataag cccaccatgt gatcactaat ggaaagatga cattctccat agacgcttgt
    12901 tgcaagatga agtcagtcaa tatatatcct acaccaaatc ctccgatgat tcctaaaaga
    12961 gtaaagatga tattttctcg aacaatgtac atcgtgactt ctttatcaaa aaatccaagg
    13021 acttttattg tagataattc gcgtacacgc tcggaaatat tgatattagt tagattatac
    13081 aagacgataa aagccagcag tcctgataat acgacaaaga ttaagacaac cgagtctaag
    13141 ttcgccattg attcttcttg cgtttcgatt tgtttggaca tgaaagacgt atttgtcaca
    13201 gcgtctgtgg caagcagttt ctcggaaagc atttcttctt cttttgttgt catcttatct
    13261 gtacgcagca aaaatgcatt atccatcatc gattgatcag taactgaagc ataatattca
    13321 ggggacatat aaataaagct acctagatag tttttcacga tttcttttac ttcgactttc
    13381 cattcttttt gatcttcatc ataaagcgaa atatgatctc ctgtagataa gtcaaatagt
    13441 tctgctactt tttgcgtaat agcgattcct gttttaggca aggagcgtgg gttctcttca
    13501 agatcagtta aatggataaa acgatccaaa tcagctgttt tacttggtac gatcactgtc
    13561 agactttgcg aagcatgtcc tttttttctc aattctacgg tttgagtatg aatagctagg
    13621 atctcattta ctttttcttg cttctgcagc atatccgctg ctttttgacg actatcttcc
    13681 gtggatgaat ccaatgtaac gattgcttga tagtcaatga tcgttccaaa ttgtttggct
    13741 gaaacagaac ttagagaatc tttcaatccc acgcctgcaa ccatcagccc ggcacatcca
    13801 gcaattccga taatcgccat aagcattctt gacttatatc ggaagagatt tcggtaactg
    13861 attttttgat taaagctaag gcgtgaccac agcggagtga tgtattcaag aaaaatgcgt
    13921 ttccctggct tcggtgcttt cggttgaagc aatgcagcag gccgttctct taaatctttg
    13981 aataaaacga tcattgcagc gccaaatgcg gcaatgaaga atgcggcagc tgcttgtaaa
    14041 atcggcggcc agtcataaaa aataagcgca ctcccaatat cataccgctc gttagacagc
    14101 tcaaaaatga tccttggcaa aagattcgtt cctaatatcg ttcctaaaac aatacctatc
    14161 cctgaggcta gacttgcata gattgcatat ttttttgcga tttcaacttt cgtataacct
    14221 aacgccttta atgtcccgat ttccctgcgg ttttcttcta ccatacgtgt cattgtcgta
    14281 aaggtgatca atgcggcaat aaagaaaaag aaaacaggaa aaacattggc aatcgcagcg
    14341 atacgatcag acagatcacc aaattcttga aaaccgacat tttctgaacg ttcattgtat
    14401 aggtatgatg gtttagttaa attttcaatt ttttctgcat ttcgattcaa ttcttcctct
    14461 gcagaggcca gttcacgttc tttctcaact aattgccttt cttgttcagc aagttgagct
    14521 tcttgttgag ataatgtttg aacagcgatt gttcgttgcg cttcaggaag ctgctggata
    14581 gttgctgact gttgcgctaa ctgtttttta gcatcttcta accgtgttct tgcttgcgtt
    14641 gtttgtgctt tgccatccgc tattttttgc tgtgccggtt ctaattcttc gttggccgat
    14701 tttttcagct cttctaagcg ttcttcgggt cgatcttgta aatacttttc tgccttttct
    14761 tgattttgct ccattttatc tttgtaagct ttgctgtacg tattcaaatt ctttacgttt
    14821 gtaaaatcaa gatataagac agaataaata tctgttttaa aaacggattc aggtaaatag
    14881 acaaaataat cgacgctacc atttccgacg ttcgcgtatc ctctctccaa attgttgatg
    14941 aacaatggag aacggacaaa gcccgtgatg gtatactctt tttctttcaa ttcatctgaa
    15001 tcaatctgat aaatatcacc gattttatac ccttcatctt ccgctttttc atccaataca
    15061 agctggtttg gtttttccgg gagttttcca tcagttaatt gtagcttgtt ctgcttgtta
    15121 tttttgttat aagaaaatat ttgaatcact tcgtttttgc cagtattgga ataatagatc
    15181 tgttttcctg tttctacttt tgcatctggg atcttttctg cttgagcaat atctttttgt
    15241 gtcaagccac cagtagaaac gatttgcaaa tcagaaagtc ctgctgtacc aacatagtga
    15301 tccatcgttt tttgcatgac aggtccagca gttttgacac ctacaaataa aagtgttcct
    15361 agcatgacaa taaggatgat tgcaatgaat cgtcctttag attgccgaat ttcgcgaaaa
    15421 cctgctttaa gatatgttct tgttttcata aaatccccct accatacaat ctcatcaatg
    15481 gatgaaggat gatcatttaa ttcaacagac cttactttag catcattaat gtgaatgact
    15541 ctatcagcga tgggcgctaa agcagaatta tgtgtaatga tcaaaacggt attgccgtgt
    15601 tgtctgctag cgttttgcaa taatttcaat acttgcttgc ccgtttcaaa atccagcgcg
    15661 cctgtcggtt catcacataa aagaagtttg ggatttttag ctagtgcacg agcaattgaa
    15721 accctctgct gttcgcctcc tgataattgg gacggaaagt tattcaatcg atgagctagg
    15781 cccacttgcc tcaatacctc tacaggatca agcgcatcag gagaaacttc cgttgccaat
    15841 tccacatttt cttttgctgt tagattagga acgagattgt agaattggaa aacgaaccct
    15901 acatctgttc gtcggtaagc agtaagctgt ttatctgaaa attgcgcaat atctgtgtca
    15961 tcaataatga tctgaccttc atcaggtgta tccatcccac ctaaaatatt aagaatcgtt
    16021 gactttcctg ctccacttgg accaagtata atcactaatt cgcctttttc aattgaaaaa
    16081 gaaatatcgt catttgctgt aatggtcgtt tctcccattt tgtatcgttt gtattcattt
    16141 tttacttcga catacttcac tctgattcct ccattttcac gatatttctc gctatatcta
    16201 tgcatcgaat gcgttttctc acacttattt attttaccac tgaatttctc tagactaaag
    16261 aaaggaatcc tttaatttag ttgacaaata aacgatttat gatttactta cataaaaaac
    16321 attgttattt gaaagaagga acatcatgaa tgatttaaag gaaaatatga tttttgattt
    16381 atcaaaaact gcttttgtcg cgatcgacat gcaaaaagag atcgtaaata acggtacact
    16441 ctctccacac actgcagcat ctattctgac aaacaatgat ttgttagtaa gaacattgaa
    16501 acgtaccgct gccttgaaag ttttagtgaa cgtagacatc tccactttcc cttatttgag
    16561 tcaacaaact gatatgggtg gaatgactgc ttctgtacct ccagaattca ctgatttact
    16621 tttgaaggat tctttgaaag atacagataa tatgttgact atcacaaaat acaatccaag
    16681 tgcttttttt ggtacttctc ttgatcttca acttcgccgg cgtggaatcg aaactatcat
    16741 tttaagcggt gtagcgacaa caaacggtgt atatgctact gcattagatg cctttcaaca
    16801 cgggtaccat atcgtgcttg ctgaagatgc ttgcagtgat cgtgacaaag aatcccatca
    16861 attgttcata aaaaagatat ttccgaaaac tgcacgagta cgctcaacga aacaaataat
    16921 cgaagctatc caacaaagca aataagtact ggcacacgtg attttcattt acttcttttt
    16981 gatttcatgg gaaaataacc acaggattta gataggagta tgaacatggt gaaaaaaaag
    17041 atcacaatca aagaagtcgc taaactttcc ggcgtttcaa tcacaactgt ctccttgata
    17101 ttaaatggaa atacggagaa attcagtccg aaaactgttc agaaagtttt agctgccaaa
    17161 gaagaattag gctatcagcc tgactatttt gctagacaaa tgattaccaa ggaaacgaaa
    17221 acaattggtg tactcgttcc tgatatcacg aatccattct tcagtacact gatgcgagga
    17281 attgaggata tcttgtataa gcagaatttc gtcacgatat tatgtaatgc tgattctgat
    17341 catcaaaaag aaatcgaata tctagccgag ctcacacgtc gaggtgtgga tggattcatt
    17401 atcgcgactt ctgctgtctc tacagatgca atcaacgaaa atttgaaaaa acaaggccgg
    17461 cccttcattg ttttggatca gaaaaaatct gaaggattta gtgatgccgt acgaacagac
    17521 gattttcgcg gtggttacct agcgggcatg cacttgctta gtctaggaca tcaaacgatc
    17581 gcccttgtct atcctgaaaa tccaccggaa aatgtccacg ctcggattga ggggtttaaa
    17641 tctgctcttg atgtttacca aatccctcac gaccaactga tcctcttacc tacacagttc
    17701 tcaaaacaag gtggctacca gatcacagca gaacttcttg attctgctgc aactggtgtg
    17761 tttgctttaa atgatgaatt agcttttgga ttatatcgcg gattagagga agctggaaaa
    17821 agcatccctg aagactacag cattattggc tatgataata tagatatgtg cgagtatatc
    17881 aagccgaaac tgacaacgat cgcacaacca atcttcgaat tgggacaaac atctgccaag
    17941 ttattgttag atcggatcca attcccagaa aaagaatggg aagaaaaaag attacctgta
    18001 cgattagaaa aaagattttc aactgcaccc ttgaaatagc tttcaaggtg tgctatattt
    18061 ttatcgaact attaaaacgt tttactaaaa cgttttaaaa aataattttc ccatcaaaat
    18121 aaatgagata gttggaggtt tgtaaaatga acacggttac agtaattgga agtattaata
    18181 tggacactac ccttcgttta accaacatgc ccaagccagg cgaaacgatg catgcgcatg
    18241 agatttttca cgctggcgga ggtaaaggtg ccaaccaagc agtggcagcc aaacgttcag
    18301 gcgcacggac atcttttatt ggaggcgttg gggctgacag cgaaggacaa cagttattag
    18361 atctactgac aaaagaaaac atcgatacat ctgggatagc tgaaatccaa ggcgccacaa
    18421 ccggacaagc aatgattatg gtagacgcag caggagaaaa tagtatcttg atccatgcag
    18481 gggcaaataa tgctttccat gaacaagaag tcctaaaaaa caagcaactg attacaaaca
    18541 gtgactttat tattgctcag tttgaaagta gcttagatgc gacgatccta gctttttcta
    18601 ttgcaaaaga cgcaggaaaa acaacgatct tgaatcctgc accagctcgt gaaacgattc
    18661 ctactgaact acttgaaaag acagatatca ttataccaaa tgaaacagaa acagaaatca
    18721 ttacaggaat cagagtgaca gatcataaca gtttggtcgc agcagccgaa aaactccatg
    18781 aattaggtat tggaacagta atcattacac taggcagtgc cggcgctttc tatcatacag
    18841 aaaaagaaca cggtatcgtc cctgctttta aagtggatgc cgttgacaca acagccgcag
    18901 gtgatacatt tattggcgca ttaagcagca cacttcagcc agaccttagt aacttgaaag
    18961 aagcgatcct ttatggaaat ctagcctctt ctgttgctgt gcagtcttat ggtgcgcaac
    19021 cttctatccc ttaccgcgaa gctttagaac aatcagcagt ctaaaattaa caatgagagg
    19081 aagaaacaaa catgaaaaaa ggaaaagtaa tcaattcaga tatttcacga gtaatcgcac
    19141 aaatggggca ctttgataaa ttaagtatcg gggatgcagg catgcctgtc ccctttggta
    19201 ctgaaaaaat agatttggct gtctcaaacg gtcttccaag ttttttatct gttttagaaa
    19261 acgttttaga agaacttgag gtacaatcga ttgaattggc ggaagaaata aagacaatga
    19321 acccggaaat attggaaaaa atcaaattat tgttaccaga tacaccgatt agctttatcc
    19381 cacatgaaga aatgaaaaaa gagttgaata actgtcatgc atttattcgt acaggcgaaa
    19441 tgacacctta cgccaatatt atattagtga gtggagtggt cttctaatgg aaacgacaat
    19501 gaatcctgaa cagatggtac tgtccgagca gattcatatg ctgattgaac agttgggagg
    19561 aaaagacaat attgattctg tccttccatg tgcgactcgg cttcgtatca aattgaaaaa
    19621 acagccagta gccaatgcag aaaaattaaa acagcaaatc ccttccatta agtatttcat
    19681 tttactcgat agatatgcag aaataatcat ggatcaggga ttggctcctg cttacaagga
    19741 attgacgcgt caaatcgatc attcagctcc gaaagaaaag ctaaatccga tcgaaaaact
    19801 atttggtttg atatcggaaa tctttacgcc gatcattccg ctgtttgccg gttctggtat
    19861 attaaaaggt ctggttgttt tagcgactac gattggctgg cttagtgaaa caagcggaac
    19921 ttatcatatt ttgtctgctg cgtcgaatgc cgtcttttat tttctgccaa tctttcttgc
    19981 tttttctgct gccaaaacgt ttaaagtgaa tggatatatt gcggctgtca ttgctgcagc
    20041 actgattgaa ccgaatctga cctctttact ttcagacagt ggcaaagcag ctactgattt
    20101 tctggggatt cctgtagtac tgatgcagta tacgtctact gtcatgccgg cgattttagg
    20161 tattttcttt tattcttttg ttgaaaaatt cctcaaaaaa tatattcgcg aaaatatgca
    20221 attagtattt gtacctttac tttcattagt tatcacggtt cctttgacgt taatggtatt
    20281 cgggcctgcg ggtgtttacc ttggcgaagg tttagctgcc gcaattactt ggatgatgga
    20341 tattaacgga ccgatcagtg gcgcaatcat cgctggtggt tggaatattt tggtaatatt
    20401 tggtatccaa tgggctgtaa accctgtcat gatcagtaat atctctgctt atgggttcga
    20461 ccgaatcgtt ccgttgacag gagcagccaa ttttggaatg gctggtgcag ctttaggtgt
    20521 atttctgcga agtaaacgtt caaaaacccg ttcgatctcc ggttctgctt ttgcttcgat
    20581 tttacttgcg ggagtgaccg aaccaactgt ttacggaatt gcgatcccat tgaaaaaacc
    20641 atttgttgct gcttgcattg gtgcagctgc tggtggagct gtaatgggat ttgcccaagt
    20701 gaaagcgatc gcttttgtat ttggtagtct gaccacattg cctgcattca tttccggtac
    20761 ttttttctgg tacttagcag gactggctgt tagtctagtc gtagcaatga tcactactct
    20821 cgttagcggg tttgatgaag acctaatgtc ttatgaatag ttaatgaaaa agactgaagt
    20881 atgagatgtt tatcacatat ttcagtcttt ttctttttag gatcattatt tttataaatt
    20941 tttgtgatac tttccaaact ttggtataat ttacatatgc ataaccattt ttatccccac
    21001 ttgtttatcc ccaaacaaag gtggtctatg caataaaatg aacgtcaaag aaaaacgaat
    21061 gaatgctcgt tgcttattta caaattgttt tatttagaat acacagacag atttatgaaa
    21121 agaaaaagct tttcgacacg attatctgtt ccaaaaaatg tctaatgaat gctgaataag
    21181 acagaaaatc aatgaaagga aaaggactag tttattatga aaaataaaaa aacgatacgt
    21241 ttgctagctg ctacttggct gattgctccg tctgttttgt cgaccaatac cgtatttgcc
    21301 gtagataatc aaatagctga tcaaacagtc acaacagata cgactacatc cagctcagct
    21361 actagtgaaa caacggattc ttttacagaa gacccaactg gaacaactaa ctcaacaaca
    21421 ggatcgaccg agccaactga aattgatccg gctgatccat cagaaaatga aaagccagcc
    21481 gagacatatt cccttacaat tgctccttct ttgattggta agatccaact gactgcttcg
    21541 acagcggatt cccctgaaca gcaacctttg gatattacgg aaagtggaac ggtagaaggt
    21601 ttattagcgg aaacgaaggt aacttacact attacgccag ctgaaggtga acaaattgct
    21661 tcagtcgctg ttaatggtgc caaagaagta aattatgatg caaagacctt tacagtaggt
    21721 acgaacaatt tatctattac tgcagaattt aaagctggac cggatacttc aacagagaaa
    21781 ccaactgatc ctagtgaaga agtccctagt acaccagatc cggaaacgcc aaacaaacca
    21841 gatgtgggta ataacaacaa taataatggt tcaaataatt caaacaataa taacaataat
    21901 tcgaacagcg ggaattcttc gaatcaaaca aacccatctg gatcgaataa caatggaaat
    21961 actcagagag aaccaaacca tccaaacaat ggttcatcga atggcggaac gaacaatgct
    22021 tcatctcgtc caaatacagc aaatcaaagc ggtatagaga atccatcttc tgcttcttct
    22081 gattttgttg taaaatcacc aattgatgct gtattgccta caaatacaac gaatgtccaa
    22141 caagcaatcg ttcgagaagc attcaatcat cttggaaaac catatgtatg gggagcaaaa
    22201 ggtccaaata cttttgactg ttctggtttg acttattacg tatatatgaa agcaaccggt
    22261 cattatatcg gtggctggac aggcgaacag caatatgcag gtacgcagat tccagtaagt
    22321 caggcccaac ctggcgacct cgtcttctgg ggaccttctt ccggtgttac tcaccatgta
    22381 ggtatctata ttgggaacgg acaatttatc catgcgcctc aaccaggaga taaagttcga
    22441 gtaacttcta tttcagattt cacgcctgat tttgctgtcc gagtaaactt agcaggttta
    22501 cctgctgctt caggttcgct gacaggcggc tcatctattt tagatggatt agatggctcg
    22561 ttccatttca gtcaaaacca aacaactgat cagttcttga aaaagattgc agatgatgca
    22621 caggaaatcg ggcaaaaaga aggaatctac gcatcagtca tgatggctca agctatcctt
    22681 gaaagtggat ctggaaacag cttattatca agcgaaccaa atcataacct ttttggtatc
    22741 aaaggatcat ataaaggaag cagcgtgacc ttcaatacac ttgaacaaga tagttccgga
    22801 caaagctacc aaatccgcgc acaattccgt aaatatcctt cttacaagga aagtttggaa
    22861 gactatgccg acttgatcaa aaatggactg actggcaatc ctgatttcta caaaccaact
    22921 tggaagtcgg aaacaaaaga ctacaaagaa gcaaccaaat atttggaagg acgttatgct
    22981 acagatcgcc aatattcaca aaaattgaac gcgatcatcg aagcgtacga cctgacaaaa
    23041 tatgatgaac cgaaaaaaga agcagcaaca actgaagaag caaacgctga aatcctcaat
    23101 atgtctaagc gattcgatgt gccaatcaaa tggacaacag atggcgtgcc aatcacagga
    23161 ttagaatttt caacgataaa acgcgtcaac aacgcttctt ccatcttccg tgtcgcttca
    23221 atctgggatt tgtggaacaa ttttacggca agacagatac cagaaacaac tactcgtaca
    23281 gttaaagctg caactgttcc tgtactaacc ttgttaagtt tagacaaatc attctggctt
    23341 gatctgcctt taaagaaata gaataaatta aaaaggggat gtgacactgt cacactccct
    23401 ttttaattta ttctatttct ttatatttat tttgtattcc gttacaaact cgcgtacttg
    23461 gttaggttcc agagtgacat ctccaaaact ttcaagtcgc tccgctcctg gcagttcttg
    23521 agtctcaaat gtgataccgc catgatttac taatttattc ccgcgcattt ccggtccatc
    23581 ttctccaaaa tttgctgtaa aaatcacgat tcctgaagca tctgttttta cttggatcga
    23641 tactttttca tctgggcttg tcaattccgc agcatttagt aaaccagatt gttttttcaa
    23701 caaaaatgga tgatcgatgc cgcccactat cattgattgg cgaaaatcac tggcaaagac
    23761 ctcacctaat tttttaggac gttggaagtc aaatgctgtg ccacttactg aaactacttc
    23821 tcctgtaggc gttgtatctt tattgagcgt agcaaataat cggctattca accaaagtat
    23881 atgttcgtcg attgaatgtg ctggatcacc ggttaaattg aaatagacat gatttgtagg
    23941 attgaatact gttttgcggt cactgatccc ttggaccgtc actcgccatg tatcatcgtt
    24001 cgtcagcgta taccgtactt ctacatctaa agttccagga aatccgtttt ccatatctgg
    24061 gctagtctga tagaaaatga ccgatgcttc gttttcacca ttgatgactg tataatgcca
    24121 ctgcttggct tcaaagctag gtgcaccacc atgtaaacaa tgaccagtgt ccgggtctgt
    24181 ttttacttcg aacgtttcat catctaattt gaatgtgcca ttcgtaatcc gtccagccat
    24241 acgtccgatt gaagcaccga tataagggtc tttttctaga tattcttttg cttcatcaaa
    24301 acctaagatc aactctcgtt tttcattatc gattattgtt tgaaaatgaa caacccgtgc
    24361 cccaagatca ctgacagaca aagatgtccc attttgattc gtgatcgtaa tcaagtgata
    24421 gttttcgcca aaattttgtt cattgatttc catgttcctt gcaccctctt ttctttcatt
    24481 tgcactgatt ccagcataca tggaaaccgt tttcttaaca gcttattccg tgagtaacga
    24541 gaaaaaattt actatattct aataaaagac cgtgaaaaaa aggttgtgac aaaaatattg
    24601 tcacaacctc tttttccgaa caaacggtgt tcaaaagctt gcatctgcgg taataagccc
    24661 aaccaaacga aaaatatgag gagctatttt tgcttggctg ttcttattac ttgatgcagg
    24721 acagcttttt cacaatctct ttttcaatac gattccagta tataaccatg tcgtcagtac
    24781 atagtagatg aaatacaata cgaagaatat cgtaaacgga acccaaatat ctttgtatgg
    24841 attttgcatg aatagaccaa acatttgtaa tccgaataag acatggacta cacctaagat
    24901 tcctggtgct aagaataaca cgcctacttc tctacggatc gattgtttaa gcaacccgcg
    24961 tcgtgttcct atcttttcaa gcatttcata acgagcaatg tcactcttgc ttcctgacaa
    25021 gatcttgaac atcaaacagc tagcaagcat ggtcaagaaa gctaaaccta ggaagaatcc
    25081 cataaattca aaaccagaga atagaccatt atacacttca tacatttcat atttctggtt
    25141 gctgtactct actttttgca aagatggatt gttcatttta ttttgagcaa caagtgtttc
    25201 gattggtttc aggtttgctt ggaagtcttt tacctgaact agctgtaaag tggattgtgg
    25261 caagttcagt tgatcaaatt ctgattgact gactaccttg atttcttttt cttgctgttc
    25321 cggcaattca tagcttcgaa gttcctcttg tacagtcagt tcattcattt ttttagcagg
    25381 aactttttct ttcgtgatct tcggcggtgt tccatcttca tgttttatca tgatcaatgg
    25441 ttgagccgca aattcacttg cattatagta aatcgttccc gcatcttctt tttgagtata
    25501 agaagcagaa agtgttggtg aaagttcttt gattttttgc tgatcttctg cccgaggatt
    25561 attcatcact agatcatatc cagaattgtt ttctgtcaag tctgcaattt catttttaaa
    25621 acctaatcct accgtcaaag cgcccagtgc caaggcaaac agcatagcga ccattgagag
    25681 catttgtgta tattcacgaa tacgaaagtt taactgtgac aatgtgaaat tgttcaattt
    25741 tttcaatgaa atctgttctg atttctttag taatgataaa aagaaaatga tcacagaatg
    25801 gaaaatgaaa taagttccta atacgatcgt aactaaggct acccctatac caaagacttg
    25861 ataggtcccc acgtgtttca tcatatagta accgcagcct agaaaagcaa ttcccagtac
    25921 tacttcaagg aaaaacaaga atttatgctg cttgattctt gttggtgtct gatccgcacg
    25981 aatcaatgct aaaatcggtt ttttgacaat cgaaaaagca ttgacaacag cagctaataa
    26041 aaacaaaaca gcaaaaaaca aaacagtgat caacaacccg tgaatactaa aaggtgtaaa
    26101 atgcgaaata ttgagatcta actgatccac taataatcgg tggacgatac ttgtcaatcc
    26161 tacaccgaca gctgaaccca ctaaagtagc tgcgatccct actaagaatg tttcagaaaa
    26221 aatcatctgt gcgatttttc tgccttttgc tcccagcatc ataaacattg catagtcttt
    26281 ttgccgcatc gtcatcaaaa aggaatttgc atataaaata taaacgaatg tgatgatccc
    26341 tagtaaaaca gaaccgaatc ggaaaatgat aactgtagcc gcaacgatcg tattactttt
    26401 caaaaaagct tcgttgcttg ccattgattc aaacatgtaa aaaatagcag atgccataac
    26461 taatcctgag aataacacca agtagtctct taaacggctc ttgatcccag taagtgataa
    26521 tttccaaagc atagtcaatc tccccctact gttccaagtt tccaagaata gatagtattt
    26581 ctcgatagaa ggaatcacgg ctttgatctc cacgtttcac ttcttgatgg ataccgccat
    26641 ccttgatgaa taaaatacgt tggcaataac ttgcagaaaa cggatcatgc gtcacaagaa
    26701 gaatcgatac atgatctttt gtattcaatt cgtccatcgt atctaaaaga tcccgggcac
    26761 ttttggaatc cagcgcacct gtaggctcat cccctaatag aatcgttggt tgtgtgatca
    26821 atgcacgtgc ggcggcgacc cgttgttttt gtccaccgga aatctcagaa ggatagcttt
    26881 ccaaaatatg agagatggat aaacgttctg caattttttg gacacgttgc ttgatttctt
    26941 ttggtctgac gccttgtaaa gaaagaggaa cagcgatatt ttcttgtgct gtcaagcttt
    27001 ctaataaatt gaaatcttgg aaaataaagc caatctcatt cgcacgaaaa tctgctagtt
    27061 gatttccttt taatgtggtc acatcttttt gattgattcg gatatgccca tctgttggtt
    27121 tatctaatgt ggataaaata ttcaaaagtg tcgacttacc agaaccagaa gcgcccatga
    27181 tcccaataaa ttcgcctttt tctacatcga agctgatgcc gcttaatgct tgcgtctttt
    27241 tctcattgtt ttttccgtat acttttgtta cattctgtac ttcgactatt ttgttcattt
    27301 ctttccctac cctttaatta gtttttccag tgctgttttc ggcacgtctt ttttttcgac
    27361 ttcttcatac gaagtgacac ctttattttt gttccaggtg atttttaaat atgctccttt
    27421 acgaagcggt cgagtcaatt gtcccttgaa gtccagttct ttttccttac cctctttcgt
    27481 atagccaggc aggatatatc tatagttgac tgtttcttgt ccagagtctg attttgaaac
    27541 tgtttttgta ccatctgtcg taatttgtac atagtattct tctccaccca taactacgta
    27601 atctgcgatt ttcacccctg caagtaccac aacgatgaaa acaagtagac cgatcagaag
    27661 ttttttcact ttttcccctt ctctcttact ttatatactg attatagaaa aaagcgaaca
    27721 attctgcctt cgaatcaact atcattcttc tttcaatttt gtcattttct ttaaaacaac
    27781 taaaaaaaga gttcaagaca ataatccgtc ctaaactcct tcttcctatc ttacaaaaag
    27841 tatgtcactt acggtctttc atgtcgcctg tattgataac aggttgcgtt cctttgtctg
    27901 taatgattga gacaagtaaa ttattgatca attggacttt ccgatcatcg gtgaaattga
    27961 tttcttgtcc atcttcgatt tgctctaagg ccatttgagt gattgtgaca gctccttcaa
    28021 caatggtttg tcgagcagac aaaatggctt ttgcttgttg acgttggagc atggcactag
    28081 cgatttctgt tgcataggct aaatggttca agcgtgtttc gattacttcg acacctgcta
    28141 ctgctaaacg ctcttgcagc tctttttgca actcgtcaga cactgccgtc gtgtttcctc
    28201 gaagcgtcaa atcattctca ttgaatgtat catacgggta ttggctagcg atatgccgaa
    28261 tggctgtctc actttggatt tctacaaaat cttgataata cgctacatcg aataatgcct
    28321 ttgctgtatc aacgacttta aagactacta ctgctgagat ttcgatgggg tttccatcca
    28381 aatcattgac ttttaaaaca gaactattga aattccgaac tttcaacgaa acagtcattt
    28441 tttgtgccaa aggtatcgta aggaaaaagc cattttctcg aatcgtacct aaatattgtc
    28501 caaaaaacaa aattactttt gcttgattag gactgactac agttgccgaa cttaacaaca
    28561 ataacgtgat caaccaaaga aaaatactta aaaaaagtgc ccaaactttc gcttgccaca
    28621 tacctaaata gaagaaaaac aatccaacta aagctaatac gaccaatccg acaatccctg
    28681 cataaccatt gatataaaat gctctttttt ctctcatgct acttcctgcg ctcctttatc
    28741 cttcaataaa tatctaatgt tactttttta attttttttt cgaatacgcg ccattacacc
    28801 ttctagttct aactctttgt cttcagtgat gtaccaaccg cttgcctgtg tccctccggc
    28861 tgcctgcggt aaataatcca attttgttcg aacccagtgc ttttcttctg taaaatagtc
    28921 ttctaccaag acctctagat ccatccctgc tgaaagaggc aaatcttccc gaacagattt
    28981 aaagataaat cgttccttct tcccatcata cttcaatatt cctgtatcga taatcggtaa
    29041 agaataataa tccagctctt tcttcaccgt caataaatgt ttgaaaactt catggtacac
    29101 ttgctctaat tggcgacctt ctgaattgtt cgggtaatta accatcactt tttctttgtc
    29161 tggatcatag aaggtttggc ttagttgagt gatataagta tgtaatcttt ccatttctgt
    29221 ttttaacttt tctctttgac cttcttctac cttcatatac tctttcaccc tgttttcttt
    29281 ttagatattt tctttcttat tttactatag ttatgtgcgt taagcgatga aaaacagcaa
    29341 agattggaag gatctttgct gttttttctt aaaatatcca gttgagtaca ggatcaatct
    29401 gtttcattgt cctcttgatc cgtcgtaata ttgatcgatg tgtctttagc agtgatcttt
    29461 agctttttct gtccttgttc taacaaagta tttgttattg gctggtagtc tttttcaata
    29521 taagattcac cagacgtttg gatagtatag ccactgtttt ccatctcatg caaagataaa
    29581 ttgccaccgt ttaggatgac ttttccttct ttgctgatca aactgtcttc tagatggaag
    29641 ttgctgtttt cacctgtgac gatcaagtta tccacattgg cctcatctaa tcgaagtgag
    29701 cctaattcat ctttcgcagt tattgttcct cctgtaaatt cattgaacga tacccagcca
    29761 tcatgtgtct tagtattcac ttttttgatt gatacttcgt tgatatcaat tgcactatta
    29821 ttcccttcga ttgtcaattc agataattca tgatctgcag ggacaacaat agtgatttgt
    29881 gcacttgtgt cttctaaact aatgattgca cctgttgatt ttttgactgc attcacagtc
    29941 aacacgccat ttttctcctt tatttctggc ttttctgtct tatcataagt cgtttttatc
    30001 tcaaaatcca atccgcgtat gaccgaaact gtttgatttt ctgcatggat gacgattttg
    30061 tttgcatcct tgattttttt tgtctcagta accatatcga tcaaccgagg accattatcc
    30121 caagtcagac tcttttgagc gcctaatgta taagttgcac ctgctaggag gccaccagta
    30181 agcatcagac caataccggt aatcaaaaaa tgtttcaatc tcaatttatg tgtcctcctt
    30241 caaacgcccg ctgaatgcag atctcttttt gtaataaaac gtcgtcccat ccatttaaca
    30301 aagttcatca tgacataaaa aagccctttt gttcctttca gcaacagtgg cgaaataaat
    30361 actgcgcttc ctgtggcgaa cagaccgaca ccgataaaga ataatccact cagaatcgat
    30421 tgtccaaaca cactgattcc tgagatgatc atgaataggc caacacctaa gatactgata
    30481 cataaaacat agatactaaa aatgagcgct agtaaagcaa ccaatagtcc cccaataacc
    30541 aatataaacc ccagtgctag tggaatgagg acaggagaag caaataaacc gataatgatt
    30601 aaccaaatca ggcgaatttg tctttttgga tttttcacta actcgttttc tgcttgttct
    30661 tcttctgaaa agtaactgat ttttaatttt aaggcaaact gctttggtgt accatattca
    30721 tcaatgatct gatctaatgt ttttccagaa tctaaaaaat attcctcata atactggatc
    30781 aaatcaaaac gatcgtcttc aggaatttct tgaagatgat ccacaacttc attcaaatat
    30841 tcattcattt gtgctctcct ccaataaccg gtctaaagaa tctcgaaaaa caatccattc
    30901 atcaataatt tcttctaaat gacgacttcc cattggtgtc aacgagtaat atcgacggtt
    30961 tctgccttca aaaggttcat cataggtctc aagatatcct tcttttttca atcttctcaa
    31021 aaccggatat agtgtagatt cactgacaga gagactttct tgtacgattt ttgttaaggc
    31081 atacccatac atatcttcct tgcttaatgc tgctagcaca gtaccatcaa gaagaacagt
    31141 tggaatctgg atgtgcatta cgtcaccttc tattctcaag tgtaatattt tatttcaatc
    31201 tatattatac aacatataat atagtttcaa aatcagtttg tcaaatacaa aaaaactgac
    31261 taaaagattt gatccttttt agtcagcaaa aaatggcttc tctttgattt ttattataac
    31321 agtggcataa tccaatttgt ccacatccag ctcagtggca tgataacgat cattgcaggt
    31381 atcatgtcag ctactgggaa cattttcacg ttgatcattc taaaaccggt agcgaccatc
    31441 aagaatccgc cgcaagcttt aaaatctaag atcatgtctg gtgtagtcaa tggataaata
    31501 aattttgcca ataagaacaa aatataaaaa ataatgaatt ggggaattgc tatcacagaa
    31561 acgacatacc ctaaattaca cgcaaaaata gctgctgtga aaaaatcaag gacagattta
    31621 gaaatcaaaa tcgtactgtc tcctgtcatc ccagaatcta aacttccata gatccccgta
    31681 ccacttgcac aaaatagaac aatgaccgtc actaatgtgc tgataaattc atcgtgggtc
    31741 ataccaagct tttcactagg gaagatttta gaaataggta cttgcatcag cgcagccccc
    31801 ttatttatcc cattacctaa atgaacaatc aaaccaatcg ctgttcctat cactaaagca
    31861 aaaataacag ccggcatatt tttcatcggc gcaatggaat agattcccat ccccatcgaa
    31921 cacacaccaa aaaccatcgt cagctgtgct ttaaaatgtt cacttaattt atttcctact
    31981 aatccgccaa ttagtccgcc aaacaaaacg gaacctacat tgatcaaaac tccagttggc
    32041 atcaaaaaac tcctctcttt ttctacgaag cgaaaaaaat cagaaaaatt tctgtaatta
    32101 ttgctttcgc tgcaagaaag attgtaacag acagagaaga ttacaccaat tcattttttg
    32161 tgttaaagta ttctttattg gaataagtag gaggaatatg atggatatcc ataaattcga
    32221 agtttttctt gatttagccg aaacaatgaa tttcaccaaa actgctgatc gacaattcac
    32281 cacccaagga aatatttcta aacagattct ttcgttagaa acagaattag acgtcaaatt
    32341 attcgaacgc gcccatagaa aaatcgaatt gaccgaagct ggaagcttat tgctcccata
    32401 tgtaaaaaat gtcgtagaac aataccatgc tatgcaagaa gtgcttgaaa cctatacgaa
    32461 agacaacaat ctttcattaa atatcttaac gataccgaca atgatcaatt ataaaggttt
    32521 ttccaaaatc accgagtttt tgaaacgcca tcctgaattt acagtccagt tgaaagaagt
    32581 ggaaagtgtc gaattaactt acaatgacgc caatgaacat agtgacacga tccactttgc
    32641 tagaagtttc caggccccac caagttcgat tgagtggttg cctactgaaa cagacgattt
    32701 tgtagcagtt ctacctaaaa atcatccttt agctagtaaa aagaaattgg atctaagcga
    32761 actgaaaaag gagaactttt tgctccttgg ccccactacg aacttgtatc aacctgtact
    32821 agatctgtgt cgcgaagctg gatttgaacc aaaaatctct tataaaggag caagaatcga
    32881 cttgatcatc aatatgattg caaatgacat ggggattgct attgttatgg aaaaaacagt
    32941 caagaatcta ttaaaagaaa atactgtgat tctccctatt tctccaacaa cagagagtta
    33001 tcttgcattt actcgtaaag tcggtgagca ttctttggct tcagatacat tttgggagta
    33061 tttaaatcaa gaagaaaact aagccctaca ttttctgtcc atctatccac ccaaaaaaag
    33121 agagcgacgc atgactttcg ctctctttct tgcatcatgt ccttgtttca atgacacctc
    33181 taacatttct cgtagttcaa taagaaagca gcgattttta tgccaaaaga accgtaagaa
    33241 catttcccta atcacaaaaa aacgactaaa aacccctaaa aaccttcaat agaattttta
    33301 ggggttttat tctttttcct attgtttact taaccgtatc attgctgtcg ttcctactcc
    33361 ttcgatactg ctataatcaa tacgtccatc atgcagttct agaatttttt taaccagtga
    33421 aagtcctagc ccattcccag aaatttgacg acttgtatct ccttgataaa atttttcaaa
    33481 cattcgatct ctggtctctt cattcattcc tacacctgaa tcggtcactt ccagtacaat
    33541 ctctgtggct gtctcaaata gtttgatatg aatctgtcca ttgatttgat tgtactttat
    33601 ggcgttatcc atgatattca gccacacttg gtacaaaaac tcttcattgc ctgtgtaatt
    33661 tactgcagca agttcgatat ccaattctag ctgttctttc tcccatttag gctgaaaaaa
    33721 aacgattact tctctgattt gttcatctaa tcgatactct ttcttttcca gttggatacg
    33781 ctggttttcc agtttgttca attttaagat gttctctgtt aattgtgaaa gctgcgtgat
    33841 actttcgata atccgatata aaaatatttg gcgttcttcg tcagagagat ttggggcttg
    33901 taacaattga acatatcctt ggattgttgc tagtggtgtt ttgaattcat gggatacact
    33961 ggacacaaag tcattcctca gtgtctcgat actattgagt tcttggacca taacgttgaa
    34021 atctttatac agttgctgta cttctgctac tttctgctgt tcatccattc gaatcgaaaa
    34081 atctcccgtt gctaccagac tcatatttgt ccctagcgca ctgattggat gcaggatacg
    34141 ccgcccaaca aaaatcgata ttcctgtacc cacgaccata ctgaaccccg caaagacgaa
    34201 taacgggtat aacgttggtt ttccagccga ttcagcaggc gagatagaat gtcgagacaa
    34261 taagaaaata aaaccgagaa aacacagcag tgtcactaag atcgaaatga agacaatact
    34321 agcaaaatag atccataatt gtgaaaatag cttttttttc atctttaaat aaccgcctta
    34381 tagcctagtc cccgtactgt gatgatttga aaatcttccg tcttttcaaa acgggtcctt
    34441 aaacgtttga tatgtacatc gatcgttcgt tcttctgtat tcgtatctaa tccccaaata
    34501 tcatccatca actgttggcg agtgaagatc ttgttgggat aagacagtaa tttatacaga
    34561 agcagaaatt ctttttgagg cagaatcaac gaatgctgat cttgtaacac tgtataagca
    34621 tctagctcta attttgtttg tccgattatc aagctactgc tgtaattgat ctttgaacgt
    34681 cgaagcaatg cttccacacg taggatcatc tcattgacat cgatgggttt tgtcatataa
    34741 tcatctgctc ctaatttgaa cccgcgtttc ttatcctcaa aatcggcctt tgccgtaatc
    34801 attagaacag gtgtgtcaaa tctggtgcta cgtaatgagt ctagcaagtc atagccatcc
    34861 attttcggca tcatgatatc tgtaatgatt aattctatac gtgtcgcttc tagttttttc
    34921 agcgcatcct ctccattcaa tgcggtgatg actgaaaagc ccgctcgttc caacacagaa
    34981 cggtaaagca actgtaattg cggatcatct tctactacta aaatacgatt cattgatctc
    35041 cctctgtttt ttgaaaaatt tctagatttt ttcttgtagt tcatcttcag ttaacattta
    35101 tttcgtatac tacgatcatt gttctgatat acttattagt ataccgaata tttcaatacg
    35161 ggagaagggt gattatgctt caattaaaag atataaaaaa atattataaa gtcggcgaaa
    35221 ccaccaccaa agcattggat ggtgtgtctg tcgctttcag acaaaaagaa ttcgtagcga
    35281 ttttaggtcc tagtggttct ggtaaaacga ctatgctgaa tgtgattggt ggactagata
    35341 attatgattc cggtgatatg gtaatcaacg gaaagtctac taaagacttt aaggacagtg
    35401 actgggatgc ctaccgaaac aactctatcg gtttcatttt tcaaagttac aacttgattg
    35461 gtcatctagg cattattgaa aacgttgaac ttgggatgac attaagtggt gtttctaaag
    35521 acgaaaaacg aaaacgggca gaagatgcat tgcaccgagt cggcttgaca aatcatatgc
    35581 ataaaaagcc aaatcagtta tctggtggac aaatgcagcg agtggccatc gctcgagcac
    35641 tagccaatga cccagatatc cttttgtgcg atgaaccaac cggagcgtta gatacagaaa
    35701 caagtatcca gatcatggaa ttgatccaag agttatcgaa agaaaaatta gtgatcatgg
    35761 tcacacataa tcctgaactg gcccatcaat acgcagatcg aatcattgaa ttttctgatg
    35821 gtaagatctt aacagattcc catcctcaca tcgaacgacc aaaagatgat caatttaact
    35881 tacgcagaac aaagatgagt ttttggacag cgttaaagct ttccttcaac aatatccgaa
    35941 caaaaaaagg acgtactttc ttgacctcct ttgcttccag tattgggatc atcggaatcg
    36001 cgatcgttct gtccctttct tctggattcc aaaaacaaat cgataacacg caagctgaaa
    36061 cgatggcgaa atttccgatt actatttcta aggtaacgac taaccagact cgtgacgatg
    36121 ccggattggg tgcaagcaaa gcggattatc cagacagcaa aacgattact gcaaaagtca
    36181 gcgaagaaga taaagcacag cacacgaata agattgatca aacatacgtc gattacgtaa
    36241 cagacatcga tcctaactta agtaataata tcggatttac ccgtactaca ggaatcaatc
    36301 ttttacgtga tgtaaatggc aaagtacagc ctgtcagctt ctcgaatcaa aatcctgata
    36361 cggaatctct ttctttatct agtacgatgt cagcaatgac aggtgtggga gtgtcaagtt
    36421 tccctacaca gcttgacact agcaaagaaa actttttaaa agacaattat tctttacttg
    36481 ccggaagtta tccagcctca gcaacagatg tcgtgctgat cgtagacggc aataataata
    36541 ctaatatcaa cgcattgaag aatcttggat ttgatgtgaa agaagacgaa aaacttgatt
    36601 ttgatgatat cgttggtaca acattcaaat tagtaaacaa caatacttac tatacaaaac
    36661 ttccaactgg gaattttatc cctaatacag attatgatgc gatgtatcag aatgcttctg
    36721 atgaattgaa gatctcgggg atcctacgcg tgaaatcttc ttctacaatg aatctcttat
    36781 ctccaggaat cgcttatagc gatcaattaa cgactcaaat cgtcaacgaa aacaaagaat
    36841 cagaaatcgt caaagctcaa agagacagtg gtgtaaatgt cttaactact gaaaaagttg
    36901 acgaaaatgc caaacaaaca ttgcttagtt atttaggtgg agacagcttg ccttcaagta
    36961 tcatgattta tccgaacaat tttgaagaca aagaaaagat cttggattat ctagatgatt
    37021 acaataaagg caaatcagat gaagataaaa tcatttatac tgatttagct ggaacgatga
    37081 ctgaactgac tggcggattg atggatgcta tcacttatgt ccttattgct tttgcaggga
    37141 tctctttagt aacaagtatg atcatgatca gtatcattac ttatacatct gtgattgaac
    37201 ggacaaaaga aatcggtgta ctaaaagctt taggtgcacg taaaaaagac atcactcgcg
    37261 tatttgacgc agaaacgtgt attttaggta tttcttctgg gatattaggt gtgtttattg
    37321 catggctagc tacgttccct atcaatagca tcttatacaa catgacggat ctaaaaaatg
    37381 ttgctcaact aaatcctgtc catgcgatta ttctagtgat cgtctctact atattgacga
    37441 tgcttggcgg tcatcttcct gcacgaatgg cagcgaaaaa agacgccgct atcgctttgc
    37501 gtgcagaata aggttgtgac aaccacattt agatttgagt cataagagcg ctgaaatagt
    37561 agaaaacagc aagaaacgag gaagtaatat gttccttgtt tcgcgctgtt ttttttaatt
    37621 attttttata attctggtat acttttcttt cctttgtacc acaactcttc tggcgtatac
    37681 ctgtcttttc catttatgaa tactgttcca tctgcttcaa tcgttacttc agtcccaatc
    37741 cacatattac gagaaagtgc gagaagattt tttctttttc ctcttccttt ttttccaaat
    37801 tgcaaggcat cggttatctc ttctgtccaa tgagatttca cgtattttac ttctccgttt
    37861 tcaaatgtaa ctttcaaaat attcgctata ttttccactt ttactgcttt aggaaatgac
    37921 tcctccattt ttttcacttt ccttcctcca acactaagta tgcgctttct tttagtgata
    37981 agtatatcat agtttattct attatcaaaa aaacgcatcc attccattgt agaatacttt
    38041 accgaacaag ttgaattgtt ttcctctttt tcacctttta ctatagatag gtaaagacaa
    38101 ctaaggagga atctctcatg acagatcaac cgtatgattt aagaaaagtc atcgaagaac
    38161 taaaaacact tccaggtcaa tatcacgaaa cagatattga aatcgatcct gaagcagaat
    38221 tatctggtgt ttaccgttat atcggtgctg gcggtacagt aaaacgtccg actcaagaag
    38281 gaccaactat gactttcaac aatatcaaag gtttccccgg cacacgtgtg aacatcggaa
    38341 caatggctag tcgtaaacgt gtaggtgaaa ttttgcacca tgattatcga acacttggtc
    38401 gtttcttgaa agacgctgta gaaaatccaa tcaaacctgt gaaagtagac aaagcagacg
    38461 ctccagcaca agaagtcgtt catctagcta ccgatgcaga cttcgatatt cgcaacattt
    38521 tgcctgcacc tacaaatact cctgaagatg ctggtccata catcacaatg ggtgtagtct
    38581 taggatcaga tccagaaaaa acaatgagcg atgtaacgat ccatcgtatg gttctagaag
    38641 ataaagatac gatcggtatg tacatcatgc ctggcggacg ccacattggt cacttccaaa
    38701 aacaattcga agccttgaat aaaccaatgc ctatcactat caatattggt ttagatccag
    38761 caattgccat tggcacgact ttcgaaccac ctaccactcc acttggctac aatgaactat
    38821 ggtgtgcagg agctttgcgt aacgaaccta ttcaaatcgt tgatggtata gcagtcgatg
    38881 aagtgggtat tgctcgtgct gaattcatca ttgaagcaga aatcatgcca aatgaaacaa
    38941 tgcaagaaga tatcaacacg aactcaggaa aagcaatgcc agaatttcct ggctatactg
    39001 gggatgctaa tccagctgta caagtagtga aagtcaaagc agttactcac cgaaaagaca
    39061 atccaatcat gcaaacaacg attggtccca gtgaagaaca cgtatctatg gccggtattc
    39121 ctacagaagc aagtattctt gccttggtcg ataaagctat tccaggaaaa gtagtaaatg
    39181 tctacaatcc tcctgcaggt ggtggtaaat tgatgaccat catgcaaatc cgtaaagaaa
    39241 acgaagcgga tgaaggtatt cagcgtcaag cagctattct tgctttatca gccttcaaag
    39301 aattgaagac agtcatttta gtcgatgaag atgttgatat tttcgacatg aacgatgtag
    39361 tttggacttt caacactcgt ttccaaggag ataaagatat catcgtgatc ccaggaatga
    39421 ggaaccatcc attggaccca tcagaattac cagaatataa tcctggctct attcgtttcc
    39481 gtgggataag tgcaaaaact atctttgacg gtacagttcc tttcgatatg aaagaacatt
    39541 ttgtacgtgc aaaattccaa gaagtagaag attgggaaaa atatttgaaa taatgaatga
    39601 tctgcaactg ttttgacatc ataaaaagga gtgcggaaaa tggaacaatt ggcacaacgg
    39661 aagaaaaaaa tcgtgattgg tgtaagtggc gcttcaggca ccatttatgc catcaaccta
    39721 gtgaagaaac taaaagaata tccgatgata gaaacacacg gaatcatcag tccatgggca
    39781 aaacagaact taaagctgga atccgactta tcactaactg aattcgaaag cctgtttgat
    39841 taccattatt caaacaaaga tttaggtgct gctgtcgcca gcggatcatt tttgaccgat
    39901 ggaatgatca tcgtaccggc aagtatgaaa acagtcgcag gtatctctgt tggtttaggc
    39961 gacaacctga ttgcacgtgc tgctgatgta acaatgaagg agcaacgaaa gttagtcatt
    40021 gttcctcgtg aaacgccatt aaatgccatt cacttggaaa acttgactaa attagcacga
    40081 ctaggtgtgc aaatcatacc accaattcct gctttttata atcagcctca aacgatccaa
    40141 gatcttgtta atcaccatac aatgaaactt ttggatcaat ttgaaattgt aaatgagcat
    40201 tcaaaacgtt ggcagggtga ttaatatgga aaaagaaacg atagttgaag taaccaaact
    40261 aggtttcacg atccaagctg ctgttaaagt aatcggtaaa gacttattga tcactttaac
    40321 aggcggagat acaccacata tcggaacagt cactacattt tctcgtgata cagagatgca
    40381 aactgtacgt ttccctagcc acgatggtcg gttccataaa gacgatgtct tagccgaaaa
    40441 aatagctgtg atcatcaaaa ctgccgttcc gggaagttgt acgatcactt ccggtatcca
    40501 tgttgaccat atctccaatg cacaaatcaa cgcctctttt ccgatgtcag aagagttagg
    40561 ttacaagatt ttagattggt tggaaaaaat agatttccat atcaaagaac cagtttatta
    40621 taaagatggt gaaaaaccat tatgaaagaa gaggttgtga aatcagcgtt atgacctgag
    40681 tatgagagaa aaaaatggaa aaatagcttc tcatattttg ccgtttttga gctaataccg
    40741 agggacagct gattgaacgc cgtttatcaa gaggttgtga taaagccgct tcgtttcgag
    40801 tagtaagaaa aattttgtaa acatgggttt ccacattttt gcataaattt gacttactac
    40861 cgagaaactg gcttttgaac accgtttatc aagaggttgt gacacgtctt ttgtcacaac
    40921 ctcttttatt tttgcttcac tgtaactgct acaatatcac cgatttcttt tccgatgttt
    40981 ttacgaatat cttttcgtag tccaaggatg tagcagactg aaccatctgg atttttcacg
    41041 cccatattca cgatactgcc ttcatatggc acattgtcaa aagttgcttg gactttaact
    41101 cttccagcac cgaattcttt tcttatatca tacggaaaaa taatatatgc cccgcccttg
    41161 ccgacttcgg aagcatagat ttttgattca aaagaataaa tttccctcat tttatttctc
    41221 ctatttttta catctagtat agcatagcaa aaaaaagaag gaactgtgac aaaagtcttg
    41281 tcgcagttcc ttttttcgaa taaacggtgt tctcctgcca actcctcggt cacaagtcac
    41341 aatagtcaac aaacatgaga agcagttttt tgaaattgct ccttgtgact cagagttaaa
    41401 cggcaggctc acaacctctt ccttaaacgg tgttcaaaag cttgcatcta cggcactaag
    41461 cccaatcaga tgaaaaatat ggagtgctat ttttatctga ttgttcttac tgcttgatgc
    41521 agagcagctt tttcacaacc tcctacatgg tgttcaaaat attattcgcc ttttgcttct
    41581 ttcactaatt ttgcgaatgc ttgttgttgg aattcagcag ctaaggcttt cgtacgttgt
    41641 tcttgctctg ctttgctcat ttttggattt tcagtcattg cttttaccat accgtcttga
    41701 acataagctt ttgcttcact ttccatcgta ctttgctgtt tcttcactaa ttcagctaac
    41761 ttggctgctt ggtccgtact taattctacc catgtctctg ggattttgaa tgtattcact
    41821 cggctgtcat attcgtgatt gttatcagca atattgaacc agaatttata catgtcgttt
    41881 ttgtattccc aataagtagt tttcgtcact ttttcagcat cgccgtctgt cttttctact
    41941 ttatttgttt cgttatccgt accagttgct tttggttttt cttgtttttc atttgttcgg
    42001 taaagataca ctttttcttt tccattccct aaagctttat acaaaagcat atctgctcct
    42061 tgggaatcag cactggaaac aattttttct gtttttgttt ccgttacttt ttccattcca
    42121 aagtgattgg acaaattagc gacgatcaaa cttagtgaag cgacgaagat tagtccgaaa
    42181 atcaaagata aaaaggtttg ccatgttttt ttagcgaaca cgttcgttaa agcaaaagca
    42241 agcaagctga caatcaaaac tccaataatc atgcattatc ccccgctttc attagaacag
    42301 gttctttttt cttcaataaa aaggcaataa taaagccaag aatcccaaag atgatcgcta
    42361 cgataaacgc ggcatggtaa cctgacagtg tggcattgat tgcctgatct ttataagcta
    42421 atggcgtatt atccaataca gatttgcttg ggatatttgc ttttgtcaca ttcgtcaaca
    42481 cactgatcaa aatagctgta ccaatcgagc ttgcgatttg acggaatgta ttatttaccg
    42541 ctgtcccatg actgatcaag ttatttggca atgcattcat accagaagtc gttaccggca
    42601 tcatgaccat ggagatccca aacatacgga tcgcataaag gatcacgata tataaaactg
    42661 gagtcgtttt tgtcaagaaa gcaaatggtg cagttgccgc tgtcaaaatg aacatccctg
    42721 tgatggctaa acgtttcgcg ccgtatttat cgaaaatcgc acccgtcaca ggcatcatag
    42781 cccccatgat caatgcaccc ggcaacaaca tcaagccaga atggaatgct gattctccac
    42841 gaatattttg aatatacaat ggtacgacca tttctgcccc taccatcgcc atatttgtta
    42901 caccagctaa aacagctgca atcgtgaatg tagatgattt gaatacacga agttccaaga
    42961 atggatgttc caaatgtaat tgacgccatg agaacaaggc gatgaaaatc acaccaacaa
    43021 ctaagaaacc aattactttc aagcttcccc agccgtcatc tcctacacta gaaaatccgt
    43081 aaagtaaact accgaatcca atcgttgata aaatagcgga taaaatatca atgctaggat
    43141 ttgacaatgg aataacactt ttcattagga agaatgctaa aaccaagaca agaacaacga
    43201 ttggcagtac cataccaaac aagtcacgcc aatggtagtt atcaatgata taaccagata
    43261 atgtagggcc taaagcagga gcaagtccaa taacgatccc tgacatcccc attgctgatc
    43321 ctcttttttc tggcgggaaa atagatagca tgatattttg caataatggc atcgatactc
    43381 ccacaccgca agcttggatc aaacgaccga ttagtaatgt actaaaatta ggtgctgtaa
    43441 agcaagtaat cgttccgatc aaaaagatcg tcatggcaga aagatataaa tttcttgaac
    43501 tgaatttatt gatcagccat gctgttattg ggatcatgat cccgttgact agcaagaaac
    43561 cagtcgttaa ccattgcaca tcagacgctg aaatatcgaa agctttcatc aatgtaggaa
    43621 aagctgtcgt caataaggtc tgattcaaaa cggtacaaaa tgtaccaatt aataagacaa
    43681 caactaaaag tgtccgatta tatggttttc cgtaaatatc tacgggttgg ttgttactca
    43741 tatttctcct tctttctgaa ttttttctga aactactaga atactttagt cctaaattcg
    43801 ctatttgtca acctacttga cacataagtt aatacggata taattaaggg aagcaagagg
    43861 acaaaaggag tgacaaaaat ggttggggct tcccttaaac gtttgttgga aaatgatcaa
    43921 ttggttgttg gcatcagtga attaagtgat atcgtaggtg tgtctccacg tcaattaagg
    43981 tattgggaac aaaagggatt cattcgttcg atcgaagctg atgcgaattg tcctagaaaa
    44041 tatcgtttac ctacagtcat aaaagtagaa ctgatcaaaa aatatttaga tgacggattt
    44101 actttgtcaa aagctgcaga aaaggcagaa atccgccaaa agaaaatgca ccatgtccgt
    44161 aaagtttttt caaaagcaat caaagatatc gaattgatcg acgaacgcta tacgatttta
    44221 caaatcggta acttcaaaga agagaatgct aagatgtaca tcattcatga caacgagaca
    44281 gacgaattaa cttacaagct ccttcctgtc gatgaaaaag ctgactacga acagttgtta
    44341 aaagaactct aaacgccact aatctcctaa gtaccaagct ttttaataag tagatgctac
    44401 ataaatactt gaaaaaaggc ctgtttgaac ataagaatat ccgaacggtg atctgcgttt
    44461 ctttcatata gaaaggaatt ctccatcact gttcggatat ttttttattt ttttcggtta
    44521 ctttcttcta atacatctgt tagccaattt gcatattctg tttcccgatt gatcgcatgc
    44581 gctaaaatca ggtagtgtcc gtaattttca tgtttttctt tttcagtagg aaagatcgtt
    44641 ttttttcgct ctaatagatg aagtcgtttt tcttcatgaa ggtcacgctg ctgctgaata
    44701 atttctacta atgccggatc attcacgtct ttgacgaaat acaatttcag aacaaattcg
    44761 tctcgattaa ccggtagttc attggtgggt gtatgaatcc actctgccaa ttgttccttc
    44821 ccttcttctg ttaattcata aactttcttt tccaattttt cacctgtgat ctctacttga
    44881 tgctgaatga ttccttgctc ttccattttt gcaagttcag gataaatctg actatgtttt
    44941 gcttgccaaa attctcctat ttcatttttg aacgattgat tcaattcata tcctgtcatc
    45001 ttcttcttac ttaataaccc taacaaaata tatcgaagtg tattttttct tgccatcagt
    45061 tttccctcct tgacaaatct ttaaatccat tctatcataa atgagtaatc gaaaacatgt
    45121 agtttattac atgttttaat ttataaggag atgcttaaac atgaaagaat ttaaagaatt
    45181 ggaagatttt attggaactc actttatcta tacgtatgac aatggttggg aatatgaatg
    45241 gtatgccaaa aatgaccata ctgttgatta ccgtatccat ggcggaatgg tcaaaggccg
    45301 ttgggtaaaa gaccaagaag ctgatatcgt taaattgacg gacggcgtat tcaaaatcac
    45361 ttggacagag ccaacaggga cagacgtggc attggacttt atgccaaatg aaaagaaact
    45421 tcacggaatg atctttttcc ctaaatgggt tgaagaacat ccagaaatca ctgttacttt
    45481 ccaaaacgaa catatcgcag aaatggaagc agctcgcgaa aaatatgaaa cttatccaaa
    45541 attacttgtt cctgaatttg ctacgatcac ttatatgggt gatgcaggta tagacaatga
    45601 agatgttatt agcgaagaac catacgatgg aatgccagat gatatccgta gcggaaaata
    45661 cttcgatgaa aattataaac gagtaaaata aggttgtgaa agagaggttg tgaaatcagc
    45721 gttctgacct gagtattaga gaaagaaacg gaaaaatagt tcctcatgtt ttgccgtttt
    45781 ttgagctaat accgagggtc agctgattga acaccgttta tcaagaggtt gtgaaatcag
    45841 cgttatgctt tgagtaataa gaacgctagt tagaaaatag cttttcatat tttctaggct
    45901 agtgggctta ttaccgagaa gcagccgttt gaacaccgtt tatcaagagg ttgtgaaatc
    45961 agcgttttga cctgagtatt agagaaacaa atggaaaaat agttcctcat attttgccgt
    46021 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttatcaaaga ttgtaacaac
    46081 tgcatattca cacagttaaa ttagtgactt caaaatgaaa taatgctccc agcaaaaaat
    46141 taggctggga gcattatttc gttattctgt tcatcaaaaa tcagcgtttt gattccattt
    46201 catcacaagt tcctcctcat ttactgcttc atctttttgc tgtgtttgaa ttgtaaaacc
    46261 ttgtgaaaga tagaaatgaa tagctgattg atttttttga tagactgtca aagtcagtgt
    46321 tttattggtt tcttttattt ttttcattag ttgcgtaccg attctttgtt gccggtattc
    46381 ccttttcaca aagattcctg cgataaactg atcaatcacc ccagcaaaac caataatttc
    46441 ttgatctatt tctgctaagt aaatcactgc atctggtaaa gctttcctaa cagattctac
    46501 ttgattctcc caataagttg atggaatgaa ttcatgagaa tccaagttag catgcagcca
    46561 aatcgataga acagattcca gttctttggc agacaatttt gttgttgtgc ggatcatttt
    46621 ttctctcctt acttattcat tgattttaaa ataaaaacca tttttttgaa gctcttttac
    46681 aagttttcgc ccctcaaaaa agtatatctt ctcttgtttt acttttctaa taccaaaaga
    46741 aacagcaact ttaggatcat cagctgaaac gattcgataa gttccaccag gcaattgatc
    46801 cagcttgact ttcccatgat ggtcgctttg tactgaaaac ggcacaccat ttctagtaag
    46861 tgtttttttc ttatgtttca aaatgaatga actaccattt accggtttcc ctgacgaatc
    46921 actaataata aatgaaaaat caagcacttt tttacttaat ctatactgat agattagtcg
    46981 tccgataaac actaacagta tgactacagc tattatcgtt cctgcaagaa taaaccattg
    47041 ccgcatggtt ctttcctgat ttccttttcc ttcttctttt tttaccgatt cgttataagg
    47101 aatccgatgt cctgtcacta gcaaacgatg cgtgttgatc atatacggcg tacatgtaag
    47161 caaggtaact aagtcttctc cttctgaaat cgttagaaca gacgtgtcat ctggcagtac
    47221 cacttgtata tttttaactt gataagccaa tttctccccc aaaacagtca aaataaacgt
    47281 gtctccgttt tctaaacgat ctaattcggt aaataaccgt cgattaggta aaccgctgtg
    47341 agcagagatc actgaatgcg tcgaatttcc accaagcggg aaagaggttc cttgcaagac
    47401 gcctgctcca ttttcaagga tcctaccatt gagccgatca taaagtgaaa tatttaattg
    47461 gatcttaggt atattgatac ttccgatcag ccattcctct aattggctgg atgttgtact
    47521 ttcatttctg ttcttttcat tgaaaggatc agtaccagga ttgaatcctt ggtcagttaa
    47581 tcgttggttt ttctcctcca ttttctttcg ctgcacttca atttcagctg cggtccgttt
    47641 ttgtacttgt tcgatacgtt tttgatcaat aaaactattt aaagaatcca cataaaatgg
    47701 atataaagcg atcagtaacc ctacgacgat acatattaat aaaaacaaac gaaacaaaag
    47761 tcgacgagac tgtttttgct tactctgctt cacatgtgtc ctcccttgca agtttttttg
    47821 ttctttcttt tattatatgg gttcaggaaa aaaatagaaa atgaaagccg atgaggttgt
    47881 gaaaaagctg tcctgcatca agtaataagt acagcccaaa aaaatagcta ctcatatttt
    47941 ttgttgggtt gggcttatta ccgcagatgc aagcttttga acaccgttta aggaagaggt
    48001 tgtgaaaact gcgttatgct ttgagtaata agagcgctag tcagaaaata gcttctcata
    48061 ttttctaggc tagtgagctt attaccgaga agcagctgat tgaacaccgt ttaccgagag
    48121 gtcatgaaaa agctactttg tttcgagtat taagaaaaat tctgtttcaa aacaaggaga
    48181 tgcaacaaaa cttatgtcgc atcctctctc cttagaactt tcacttatgt ttttcttgta
    48241 aaattcataa atattttcat gaattttcta atttcttgcc tacctctcgc ataggatata
    48301 tgatgaattt atctaaagtt tgcaaaggag ttcgacaatt gaaaaaatat ggcccatttt
    48361 tagcaatttt tttcattgct ttggcatttc tcatgcctca tttattcaca cataaactaa
    48421 ttttgggtgc tgatgcccca ttccattaca atcggttcta tgatgccgcg caacaaatta
    48481 aatatgggga tttccattat tttccttccc tttatggatt ccagcaaagt ggcaggatcg
    48541 tgaatgccgt ttacggccca tttttcgctt atttacaagg attgatcgtg ttgcttgcag
    48601 gtagctggtt tcattatcag attctttcaa atttcattct ttatcttatt tcaggatttt
    48661 ctatgctgat tttactgaga tatgtcaatg ttcgaaattg ggtaagtgta ccagtagccg
    48721 ttttattcat gagtacgtat tctattcaat actggtctat caaccaagga ttcactagct
    48781 gggggacttg tttttatcct ttatgtatga tccctattgc tgatttcgtc cttcataaaa
    48841 agttccctgt tatcaaagtc gctgtctcag tgggacttat gacacagatc catttactgt
    48901 cttcgatcat gttagctttg atgtatatcc ctttttatct ttattacttt tttaaccaga
    48961 ctgaaaaagg aaaagcactg cttcaactga ttaagtcggt cttgctttat tttgttctga
    49021 cagcaaatat ttgggcagga ttagctattg tttacagtgg gaataagatt cttcctccct
    49081 ttgttaacag agaaatgagt caaaaggcga tagatctgga tggcagttat tggctccatt
    49141 accctaaggc ctttcctata ttgatattat caggtttttt gctttttatt ttctttcaac
    49201 aaaagaacac cttattccaa aaaatcgtgt tgtacacaag tcttgttttc ttttttcttt
    49261 ctactagctt gattccatgg acgacattga tcgaaaaaaa tgtgcctttt attagtttga
    49321 tccagtttcc atttcgattt ttcgtgccat ttacgctatt gcttctactt taccttgccc
    49381 tttcactgaa ccagtggtct gaacaaaagt ggttccgtct tttaagtata tttggcattc
    49441 ttattttttc agcacagacg atccagaact tataccagca tctggaaaag tgggaaaatg
    49501 aaacatttgt cagtcgacat acttatctat tcgatacgcc ggaagaagca aggaaaacat
    49561 tcttcagcaa agatatgtct accagcttgt tcgttttcca aaaaaccaca cctgactatc
    49621 tgccaatcta taaggagaca aaagacaata aatacgaccg gtataacgaa gaaatactag
    49681 aaaacgaaac aacttttata aaaacacatc caggcggtct tttgacaatc aagtggaaaa
    49741 acactgggga caaaactgtt catatccctg ttatcgtcta cgatcggact gtgctacagc
    49801 aaaatggaaa aacattgact gattatgaag taacagatat cgggacccca atcgtgaagc
    49861 agcaaaaagg aatcaacgag ctcacgcttc attatcaaac gccaatctat ttctatttca
    49921 ttctttctct tactttgatt ggctggttta ccttgctatg tttatttatt tatcatagat
    49981 acaagctttt acgtgcttag gcaaaaggga taaatacatc cgatcaaaaa actctccaaa
    50041 cggtataatc gtttggagag ttttatttta atcagttttt ttatgttata tgttatattt
    50101 tttattcgtt cacatctaat tcgatttcca ccccgtagtg atcactgatg attggactat
    50161 cgatcccatc aaaaatccgt tgatattttt taacctgcat tccttcaggg acgaaaataa
    50221 aatctatacg caatttttct gtattttgtt cccaaccatc tattttcttt tccacagtag
    50281 ctgcttttcc tttttcattc gcgacaacat acgaatcttg aagaagaagg caactttctc
    50341 ttacagtata ataaccttga gagtctactg gattattgaa atcacctagg aaaaaaagtg
    50401 gtacgcgagt ttccaaaaag tagttttcca agtttttcca ctcaaaataa aagccacttt
    50461 ttttatctgt ccaccaagaa aagtggcagc tcgagacaaa aatgtcctga tcatctatct
    50521 ttgttttccc aatcaagatc ttgcgtgtct gataatcttc tttcttttga aattgggaga
    50581 cggtaaggac ctgactttcc aatgtttttt tcgtaagcag tgcatttcct tcttcatatt
    50641 tatcataacc gatgtggctc atttcccaac tccaaaaata ctcttttccg tgttccttta
    50701 aaagctgaac taaacggtag gcaaaattat ctttacggat aggtacttga ttctttaccg
    50761 gacagaactt cattagcttc gcaggttcta cttcttcaga ttccagtaat tggttgattt
    50821 cttgtaaagc aatcacttca taatcttcta acaggatctt atctgctaat tcttgcaatt
    50881 ttttctctgg ttctggctca agccagctat gcgtgttgag tgtcatgaat ttcattggtt
    50941 ttctccttgt cttcatttcg aaacaaaagg gttgtgacaa aatttttgtc acaacctctc
    51001 cagcaaatag tgttctcctg ctaactcctc agtaataagc tcaatggacc aggagctatt
    51061 ttttgtttgg ctgtttttat cacttggtgc agggcagctt tttcacgacc tctccactaa
    51121 acagtattca aactgctgct tctcggtaat aagcccaacc aaacgaaaaa taggaggagc
    51181 tatttttgtt tggctgttct tatgacttga tgcaggacag ctttttcaca acctctcctt
    51241 acattttaat ggagccgatc actttaccag ctgtcactgt acctaattgc ttgatcacta
    51301 cttcttctac tttatcgttt gtaaatacga tcatgatcgt tggatctttt tcttcgtttt
    51361 ggatcacatc aagatccatc tcagctaatt tcgttcctgt tcctacttct tggccttctt
    51421 tgacagcaat ctcaaaagct ggctgattca tttggactgt atcgattccc atatggatca
    51481 aaacttcgat accatctttc gtttccagcc caattgcatg tttgctaggg aaaatacttg
    51541 tcacttttcc agcgatcggc gcaacaactt ctcttgatgt tggaataaca gcaaagccgt
    51601 cacccatcat tttttgtgag aataccggat cttttacttc agataaagga atcacttctc
    51661 cagaagctgc ggaaactagt tcttgttcta tacctaagta ttgcttacta cctgcttgtc
    51721 catttcctgt agaaaccatt tctggctctg gaatctctgc tcctgattgc agcagatctt
    51781 cgatatctga tttcaagaca tctgcttttg gtccataaac tgcttggact ccattgtctt
    51841 tgacgatcaa tcccatcgcc cctgctcgtt tccaggcttc ttcagtccct actttttctc
    51901 gatcatttac acttacgcgt aaccgagtca tacaagcatc gacatctttg atattttctt
    51961 ttcctcccag caaatgaatg atctggacca cttgctgatc tgctgttcct gttccgctgt
    52021 tagatacaac ggtttcttca ctgtcattgt cgtaatttcc gttacgtccc ggtgtagcaa
    52081 agttgaattt tttgatcaag aaatttgcta agaaacacgt aacaacacca aaagcgatca
    52141 cacacaacac gaagttgata agatctccac ctagacccgc tttgatagct aacggtgtcc
    52201 gagtcagcaa ttcgatattc ccaaatgaat ggacccgcaa tggcaagata tctgccatcg
    52261 caaatgcagc cccttggatc actgaataaa tgacatataa tggcacagca gcaaacataa
    52321 acatgaactc caatggttct gttacacctg ttagaaagac agccaacgct gctgaaaaat
    52381 acattgattt atatcttgct ttcttatcag gatcgacatt acgatacatc gcaaaagcca
    52441 ttcccatcaa gatacctgaa gaaccaatca tttgtcctac tttgaaacga gccggcgtcc
    52501 agttatgcaa cacaaactca tattgcgaca tatcccctgc acctttaaga ttcactaaat
    52561 cagtggccca agccaaccat aacggatctt gcccaaatac ttgtgtcccc gcctgtgcgc
    52621 ctgataaaat ctcatacgat ccgccaagct gtgtatagtt gataggaatc gtcagcatat
    52681 gatgcaatcc aaatggcaat agtaaacgtt ctaatgttcc aaataaaaac ggtgctagaa
    52741 tcggtgcgct ttcttgtgat tctgcaatcc ataaaccaaa attattgatt ccagcctgta
    52801 cgtttggcca caccacagca agaatcaatg ccacgattgt tgaccataag ataacaacaa
    52861 acggaacaaa gcgctttcca ttgaaaaatg acaaagcatc cggcaatttt ctataattat
    52921 agtacttatt ataagccatt gccccgacaa atccagcgat gatccctacg aatactccca
    52981 tattcaaagc tggtgcttcc aatacacttg taaaaaatcc tttaaccatg atcttcgtac
    53041 cgaacagagt gtgggtaaat gcatcttcat tcgcaagcat gtcacttgtg acaccaaaga
    53101 tagccccagt gatccgattg attaaaataa atgagatccc agctgcaaat gctccgcctg
    53161 ctctttcttt tgcccaactt cctcctattg ctaaagcaaa taaaatatga agattaccaa
    53221 taattgccca accaatattt tcaattactc cgcccgttgt gacaagcggc gtccaatcag
    53281 gattgatcaa gggaattgat ttccctatac tgatcatcaa cccagctgcc ggcataactg
    53341 ctaccacaac cattaaagcc tttccaaatt tttgccaaaa ctcaaaacta aatatctttt
    53401 tcattccgtt tagctccttt acgcaatcgg tttcgttaaa cgtattatag cgtgcgtttt
    53461 cttttttgta aatgctttat tttttatttt tctgtctata ccattctata aaacctataa
    53521 tacgtgtgtt cccttctata attttctcct aaaaatttat ttttttaagc ttttttctat
    53581 gaaatatttt cttttgtcta ttgacttgtg caatcggttg cgtttattat gaacgtataa
    53641 aacagctcat taggaggaga aaaatgaaac agatcaaacg actatttgct attgatccat
    53701 ggaaaatcag cacgacacaa ttagataaaa aaaatcttcg actgcaagaa tctttaacaa
    53761 gtattggaaa cggttatatg gggatgcgcg ggaactttga agaaacttac tcaggggacc
    53821 accatcaagg tacttatttg gcgggtgtct ggtatcccga taagacacgt gtcggctggt
    53881 ggaaaaatgg ttatccagaa tactttggaa aagtgatcaa cgccttgaac tttatcgcga
    53941 tagatatttc gatcaacgat cagctgatcg acttggcttc tttggaacca gaagactttt
    54001 actgggaatt agatatgaaa aatggtgtat tatctcgtac attcacaatc actacttcta
    54061 ctaataaagt acgcatttcc ttcgaacgtt ttctgagcat tgtcaaaaaa gaatcagctt
    54121 atatctattt aactatagaa atgcttgaag gtagcggaac agtcaaactc gtatcaaaat
    54181 tggatggaaa tgttcaaaac gaagacagca actatgatga acatttttgg aatgagattg
    54241 atcgtggaac tatagaagaa atcggctatt tgacgactaa aactattcca aacaactttg
    54301 ggattgaaca atttactgtt actgcagcaa tgcgccattt cgttaatgga aaatcagtca
    54361 tccctgacta tcacgaagat actttagcat tacatggttg cttgtctgca gaattatctc
    54421 aaggtgagac atggtcagtg gaaaaagaaa ttatcgttct tactagtaga gatattccag
    54481 aagcgcagca agcagaaaaa gctgtctcag aattaacgaa ccagacaaac aattatatcc
    54541 aagcaaaaga agagcaaaca gcggcttggg cagaacgatg gaagcttgcc gatgtggtca
    54601 tcgaaggaga cgaagaagca caacaaggta ttcgattcaa tttgttccaa ctattctcta
    54661 cttactacgg cgaagatgaa cgattgaata tcggccccaa aggattcaca ggagaaaaat
    54721 acggcggagc cacttactgg gatacagaag cgtatgctgt ccctctttat cttgctttag
    54781 ccaaacctga agtaactaag aatctgctga aatatcggca taatcaactg ccgcaagcaa
    54841 tccacaacgc tcagcaacaa ggattaaaag gcgcgcttta tcctatggtc accttcactg
    54901 gtgtcgaatg ccataacgag tgggaaatca cattcgagga gattcaccga aatggcgcga
    54961 ttgcctatgc aatctacaat tatacgaatt acaccggcga tacggaatat ttgaaaaatg
    55021 aaggactgga agttttagca gaaatcgcac gcttctgggc agaccgtgtc catttttcaa
    55081 aacgagcaaa taaatacatg atccacggtg tgaccggccc aaacgaatat gagaacaata
    55141 tcaataacaa ttggtataca aatacgatcg cagcttgggt cttgcgttac acgcgagaaa
    55201 attatttaac ttatcaaaaa gaagctgcta tcactatcag cgaagaagaa ctagccaaat
    55261 ggaaagatat cgcagagaac atgtacttcc cagttgacga agaattagga atctttgtcc
    55321 agcatgacac attcttagat aaagatctca tgcctgtatc cgatattccc acaagcgatc
    55381 ttcctctgaa tcagcattgg tcttgggaca aaatccttag atcttgcttc atcaagcagg
    55441 cagatgtact acaggggatt tatttcttca atgatacttt tacaaaagaa gaaaagcaaa
    55501 aaaactttga tttctatgaa ccaatgactg tccatgagtc ttccctttcg ccaagtatcc
    55561 attcgatttt agcagcagaa ttaggaatgg aaagcaaagc tgtagaaatg taccagcgaa
    55621 cagctcgctt ggatttggat aattacaata acgacacaga agacggcttg cacatcactt
    55681 ccatgactgg tagctggttg tcgattgtcc aaggatttgc tcaaatgaaa actgcccatg
    55741 atcagttgag attcgcacca tttctgccaa aaacttggaa agcttattct ttccatatca
    55801 attatcgcgg acgtttattg ttcatctcag tcacagatga acaagttact ttcgagttat
    55861 tagaaggttc tcctctagaa atttacgtct atgaacagtc ttattcttta gcggatcgtc
    55921 tagaaataac gctaagaaag gaggaagcaa atgtttaaag gggtattgtt tgatttagac
    55981 ggtgtcatta ccgatactgc tgaattccat tatcgtgctt ggcgaaagct tggtcaggaa
    56041 atcggcatta cgatcgacca cacattcaac gaacagctaa aaggcgtcag ccgagaagac
    56101 tcactctctt tgcttttagc ttatggcgga aaagaacact ctttttcaaa ggaagaattt
    56161 gcagaattag cgaaaagaaa aaacgattat tatttagaga tgatccagac gatcgaacca
    56221 aaagatgtct tccccggggt cgtgccttta ctggattctt taaaagaaga aaaaatcaag
    56281 attgctcttg cttctgcaag taaaaacggg ccattcctac ttgaaaaaat gggtcttaca
    56341 ccttactttg atgctattgc cgacccggca gaagcaacaa acggaaaacc tgcgccagat
    56401 atcttcctcc ttgcagcaaa agctgtcgga ctaaaagcag aagaatgtat cgggatcgaa
    56461 gacgcgcaag ccggtatcca agccatcctc tcaagtggcg ctcaaccaat tggcgtggga
    56521 cgtgctgaag accttggaga ggagatcccg cttgtccctg atacgacttt tcttacgatc
    56581 gattacttga aagaaaagtg gcatgaccat ggataatcca gagatctccg tcgtaccagt
    56641 tccagagact gaaataaaga aaatcacatt gaagaacgac tacttagaag tcgttcttct
    56701 caattacggt gcccgcctgc accaaatctt cgcaccagac aaagaaggaa aatcagaaaa
    56761 tgttttattg tcttacgatc gtttcgaaga tgttctaaca gacaaaagct tttttggagc
    56821 aactgtcgga cctgtagccg gaaggatccg ggatgcttct tggggtgagc atagattgga
    56881 gaaaaactgc ggatcacacc atatccacgg tggcacgaaa ggctggtctt tccaattttg
    56941 ggaagtagaa gttttcaaaa ctccatcatc aataggtgtg gtcttttact taaaagatga
    57001 attttctacc tatccaggac caatcactgc tacgatcact taccgtttaa caaaaaacga
    57061 attagagatg acaacctctg ctagtagcct tgttgaaacc atctgcaatc cgaccaacca
    57121 tgcctacttc aacctttcag gaaatggaac aagagacatc tatgaacatc agttaacggt
    57181 ctttttagat ggtgtgcttg aattagatca agaaaaactg cctactggaa attggacaaa
    57241 aaaagaaaaa ctgccaatcg actttagaaa atccccaaca ctgcaagaga tccttgcatg
    57301 ttatccagat ggactagatg atgtatttct tttgcatcat cctaggcttt ccaaaacttc
    57361 cttacaatta ttcgagaagc attctggtcg ccaaatgact atagcaactt ctaataaaag
    57421 tatggtatta ttttctacga ctggattcga agctgatttc tctgtcaatg gaaaacagat
    57481 gcattcaaac tacggattag caatcgaacc gcaagaaatc cctgatatcg ttcatttccc
    57541 caaatttgga tcgatcaacc tccatcctgg tcaagaacgg atcagtcaga cgatttatcg
    57601 gttctctgca caataacaag agaaaggtgt ttttatcatg gctattaccg taaaagatgt
    57661 ggcgaaaaaa gccggtgttg ccacctcgac cgtctctcgt gtcatcaacg atcatcctag
    57721 tatttccgag acaacaaaga agaaagtccg aaaaattatg gatgaactag gttatgttcc
    57781 taacatgact gcccgcaatc taggaaaacg catttccagt gccatcggcg taatcttacc
    57841 ccctcttgat tcaaaagagc ggatcggtaa cccattttat cttgagatca tggaagcaat
    57901 caacgaagaa gcgcggaatt atgatatgac tacagcaatc gctactgcta aaaattttga
    57961 attgctgtta gagaacgtcc agcgtatgca tttacaaaaa caagtagatg gttttattct
    58021 agtgtattca gatagtcaag atactgtcat tgattattta taccaaaacc acatcccttt
    58081 cactttgatc ggacagcctt atcatcatga gaacgaaatc gtttatgtag ataatgataa
    58141 ccagcttcta ggtaaacaag caacagaatt tttgatccag aatggacata agaatatcct
    58201 gtttgtgaca aacacgactc atgaaagttt gtatttcgaa cgatatttcg gttatcaaaa
    58261 agcaatgatg atggctggtc ttcctgcgtt atccagtgta gtactagagc atccggagga
    58321 ttatctgtcc tttgagaaaa cgttgaaaga aaacgaagcc acagcattgg tggtcattga
    58381 cgatatcttc gcccttcgca cgatgcagtt ggcgcaaatg tatggctatg tcattccaga
    58441 gacactctcc gtcattagct tcaacaactc gatcttctca acattaacac acccttacct
    58501 tacaagtatc gatatcgata tctccgaatt aggacgtgtc gctatgcaaa agctgcgcga
    58561 attgatccat gaagaattga gcagtggcgt acgtttggtc atccctcatc gcttgatcaa
    58621 aagagagaca gtcgttcctt tagaaaaccc atactaaata gctgactcac taaatacgct
    58681 gaccatttat cctatggtca gctttttatg taaaagaaga tactacttct aacacattgt
    58741 ttatctgtaa aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac
    58801 acgtataaac ggcggaagca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat
    58861 ttaaaaagca attttcttga gtttcttctt atttcttgga gttaaacgct tctgtcccag
    58921 cctctttttt ccttccttca attttttaca cgtcgacgat tcaataaaaa accgcctaat
    58981 attgtaaaga caggtgcaag aatcgagaaa aaggcgaatg gcaagtaatg caacgcgcta
    59041 actttcatcg ttcccataat gaatgtgccg ctgactcccc acggtacgag agaattgacg
    59101 gtcgcacctg aatccgctag tgttcgtgtc aaataggttt ttgataagcc aacgtcatca
    59161 aaagaacgct tgaatgtttc tcctggaaga atgatggaca gatactgttc tccaacgatc
    59221 agattgattc cgatacagct caaggcagta aacccgacta aacgagaagg tcggtccatc
    59281 ttttctttta attcttgaac gatggtatca acgatcagat atttgatcag taacccacct
    59341 aatgccaatg caagtaaaat caaactagca gaacctaaca tactcatgat cccgcctcga
    59401 gaaagcagtt catctagctt tggatcaggt gtatttgcga cataaccatt catcaagatg
    59461 tctgctgctt ttccgaccgt caaacttttc tcatggataa aagaaagaac gagagcagct
    59521 gtcgatcccg ctagtaatgt agggatagcc ggaatttttt tgattgcccc cccaaataaa
    59581 ataactagtg gaatcaatgt ccaaggagag atccaaaagc tcgctttcat gttttggatc
    59641 atttcattga tactagctag atcggctgaa tgactgttat gtcctaatat cgtataaaaa
    59701 atcaacgaaa ggacgaatgc tgggaaatcc gtataacgca taccagcgat atgttcgaat
    59761 agattaactc ctccaatacc cgctgccaag ttggtcgtat ctgataaagg cgagcagtta
    59821 ttccctaaaa aggcaccgga aacgactgcg cctgtagtca ttgcatcatt gaatccaagt
    59881 aaatgaccga tccccaaaaa agcaattcca atcgtcgaaa tcgttgtaaa tgaactgccg
    59941 acagtcactc cgacaattgc acaaacaaga aatgctgtcg gtaaaaagaa acgcacagaa
    60001 acaagtttaa atccataaac cataatcgta gggatggttc cagataaaat ccatgaagca
    60061 actaagacac caatcaaaag aaaaatcagg attggaatga tccctggagt gattcctttg
    60121 atgatcccgt catggatctc atcccatgtg aactttttaa atctgccata aaacaacaaa
    60181 cacatgaaag ctgcaaggat cgggatatgt ggcgagagct ggaatccgat gatcagtact
    60241 cctaggagtg ccaacaacac gatcaataca gcgatacttt cttttaaaga aatattttta
    60301 ttcatcgtcg atctcctatt tcaaatgaaa aggccctgcc atgaaaaaac tattcacttg
    60361 gcaggacctc ctactaaatc agcgacaaaa cgtcggctga aagacttatt tttttatttt
    60421 tcttctaaac ttttttctac ctcaatcccc caagaatctt tcttgaagtg attgatgatc
    60481 aatggaataa cgaacatgac cactgtgata gctgctacta ataatgcatc actcaatgtc
    60541 gctgtaccta atgtagcaaa tacgacaacg atgatagaga agatcagcat gcagctgact
    60601 acccaagcta atccgtttcc gtttttacca attcggtaag gacgttcgaa ttctggatgt
    60661 ttcttacgca ttttaagcaa tgaaacagca atcaaaatgt acacgataca ataaataaca
    60721 gttgtagcat ttgtaagtgt caagaataca gcacttacgt catccattac accataaagc
    60781 aaagcaaata atgaaataac gattgattgt gttagaacga catttctaga tacgccgtat
    60841 ttgttttctt tatggaatcc gaattttggt ggaaggaatc cttcacgagc tacttggatg
    60901 attgtttttg atggtccagt aacccaagct gataattgta aaagcacacc aataaatacc
    60961 atgaagctga agatatttcc gatcacttct ggtaagccta ggatttgaca gtcaatcaag
    61021 ataggctgcg tgatatttgc taattccatt tttccatctg gtacgacatt tgctacaagc
    61081 atcgcattga tgacattcaa taaaactaat ccgatcaaag cgataaatac gccttttgta
    61141 taattttttg ttgcgtcttt caaacgaggc atgtaaacag aactcatttc gatcccaaca
    61201 aaaataaatg tgataccagc taagtatttc aatgaatcaa tatgttccaa gtcagggaaa
    61261 gcttttgacc atgaaaagtc acctagatag ccgtttgaga ctagtccgac tttgaaggct
    61321 gccaaaacac ctaaaacgaa catgatgact actgggatat atacccctag ccatacaccg
    61381 atgttcccgc cgacttttgc catatcgaat ttcaagttca agatcgtgat gatccagtag
    61441 ataactaaaa tacaacctaa aacgaaccag tgattattgc caagttctac attaccaaat
    61501 gtgtttccta acaatggtcc taaagtagaa gcgaccatta ccatacctgg gaacatttgc
    61561 acccataata gccatgcaac gacaaatccc catttgctgc ccagtgctgt ttttacccat
    61621 aactgaggac cgccttcttg aggaagcatc gtggaaagct caccggaaac cattgatatc
    61681 ggaccggcaa aaaacaaaac tgcaaaaatt gaataaaaaa tcaatgtcca accgactgcg
    61741 gctaacgtag gaatactccg aaccgttgca cacagtgcca ttgtcagacc gataaaggtc
    61801 ccaagaccca ttttacttac ttcagattta ctcaccgttg acactctcct tgattttttg
    61861 gataaaataa cggtgtcttt gaagtagttt cagcaataaa cgaaacaatt cagcgaatat
    61921 ttgaggggat tctttgagtt tattgcttga agggtttctt caaagacaac ctcaaaacaa
    61981 agggatcgaa gagcttaggt ctccccttgc tcttcagatc caaacatgcg tcagaaacag
    62041 ttagtattca agctgttttc taacatgatt accttgctta ttttgcttcg cttgccaata
    62101 attggtctgc gtagattttt tccaattttt cttgaagagc tgctttgatt ttttcagcat
    62161 attcttcaaa gtgttcttct ttgttcatgt atggtgtcat tacgctggca cgcaatacag
    62221 ttacttttcc ggcacggttc cattcttcat ctgagaatcc tagttggttc acaaattgta
    62281 atgggctgtt gccatagtct gggatagcaa agtcagtatg tgaagttaag aactcattac
    62341 cgtaaatgct tcctttcacg tatgaagaat aatcatatac gtcatggttc aatttattca
    62401 ttgcaactaa gtcatcgttg cctttttctt tgaatacata gtctaccata ttgaaatctg
    62461 gataagttaa tggatgaact tcgatttctt tgtctcctac tttaaatgac aagtcattca
    62521 agaagttgta gaaacgatgt gcaccttcga ttgatgcacc cattaattta ccatatcctg
    62581 tgacattcaa aggtaagaca tggtgtgctg cccaaacgct ggctgctgtt gcccctgctt
    62641 ttgaaccttc caagatataa gccccaagta atgctgggat atctgcgcct ttttcaaata
    62701 catatgtcgc aaagtaagag ataacgtctc tcatgcggat atctttgatg acgataccac
    62761 cggcagaata tggaacatag cccattttat gtgggtcaat cgttacagat tctgcttctt
    62821 cgattgcttt atatgcactg tgtacttctt ctaagatata atctttgttt tctgtaaaga
    62881 cattgtattt gtaatgaaca tcttttaaat cttcaaatgg aatgaagttg ttgtcttcgt
    62941 ctaagaaaat cgcacggccg tatccgccat aagcagcatc tacgtgtaag tagaagtaaa
    63001 taccgtcttt ttctaatacg cgacgcaatg caacgatttt atcgataccg tcgattgctc
    63061 cttcttctgt tgatcctaca acaccgacta cacctaggat tggtgttttt tcagctgcta
    63121 gaccacgaac gattttttca agttcgttga tgtccatgcg atagttgtgg tcaactggca
    63181 ctggaatcac ttggtctaaa ccgataccga tgatgtcggc agcttttagc catgaatagt
    63241 gtttagtttg tggaaccaac cattttccta atttttctaa atgttttccg ctacgtgctg
    63301 agtgagcttt gatttcgtcg attttttctg ggacgctgtc taacagattc atgatttctt
    63361 ctgttgacaa gttcattaat tcccaatcgc tttttccagc aactagttca ggtgtcactt
    63421 ctttcattgc aagcggcaat gatttgatgt tgcgggcata ccaaagtcct tctaagtttg
    63481 ctaaagaacc atcagcaacg atgtggcccc agccatcttt atagctcatt aattttgcaa
    63541 attccattcc tacttcttct tccatttggc ttgttgcagg agaagattca taggcaacgt
    63601 tattaccatt ccatagcata gcaaaattgt acgctagtaa agatggcatc aatgtttcag
    63661 agttcatatg accccagtaa cgtccggcat tatgccaagg aacggaatgg gtacgcatcc
    63721 gcgctgaaat ttctgataga acatcctttg ttttattcac agtgtgttca aaactagcac
    63781 tgcttttttc ttctggcgta ataataggca tgtcttgagg catgtagttt tgacgccagc
    63841 ctaaatgttc atccaccaat tcattcaaca aagctttata gatttggccg ttctcagctt
    63901 tgtccccaat gaatagggca tttaggttta gatctttcga caatgattca ctcatagtga
    63961 aaccctccta aaaaaatatt taaattttgt cgagccctac attacacctg ttcttctatt
    64021 ctgtaaacta agttttggcc tgatttttaa tcatatttta atatgtatca attatccgaa
    64081 ttgttcacac ttttgaaata attaaaaaaa acgagggaat ttacaaatgt aaactccctc
    64141 gcaaagattg tattaacgac aaccagcgtt ctattttagg atgggtttca aaattggaat
    64201 gttgtaatag tatttccctt tttcaaggta aaaacttact aaattctaat caattacttt
    64261 ggccaaaaag taatttttct taccttttcg aataaccaca aacttaccat cgaattcatc
    64321 ggatggattt atgacaaaat ttaaatcggt aacgcggtca ccgttaatac ttatagctcc
    64381 gtttgaaaca tcttcacgtg cttgacgttt agatggttca atttttgtgg aaacaagcag
    64441 ttccacgata ttttcaggtg tgcttgaaat ttcaacgttc ggcattttac cgaatccttg
    64501 cgcgatttct tccgcattta gttctttaat atttccagag aacaatgctt gcgttatttt
    64561 ttgcgcttct ttcaagtctt cttcactatg cacaaatctt gtaacttctt ctgccaaacg
    64621 gcgttgtgct tcacgtttct caggttctgt ctctactttt ttcgctaagt cttcgatttc
    64681 ttcttgagac aagaaagtaa agaatttcaa gtatttcact acatcgcgat catcttgatt
    64741 caaccagaat tggtagaact caaatggtga agttttcttc ggatcaagcc aaacggcacc
    64801 gcctgcagtt tttccgaatt tcgttccgtc agccttcagc atcaatggga tcgttaaacc
    64861 gaacgcttct gcttctggtc cttctaattt tcggattagg tctaatccag ctgtgatatt
    64921 tccccattgg tctgctccac cgatttgcaa ttgcacatcg tatgttttat gcagatgcaa
    64981 aaagtcgata gattgcagga tttggtaagt aaactctgta aatgagattc ctacttccaa
    65041 acggctggca acgatatctt ttgctaacat cgtgttgatg ttgaagtttt taccgtagtc
    65101 tctcaaaaat tcaagtaaag agatttttga caaccagtcg tagttgttca caaatgtgat
    65161 atttgcatct ttaccaaaca attttctcat ttgattggaa agtgcgtcca cgttatgttg
    65221 cacttgttcc atcgtttgca acacacgttc tgttttgcgg ccgcttgggt cgccgatcgt
    65281 tcctgttccg ccgccaatca gaatatatgg atgatggcct gccaattgga atcttttcat
    65341 catcataaat ggaatcaaat gtccgatatg catcgaatca ccagttggat caacgccgca
    65401 ataaagtgaa atgctctttt cttcaacgag ctcgcgaagt ctttcttcgt ttgtttgctg
    65461 attaatggcg tcgcgccaag ttaattcatc aataatattc attttactga cctccgtgta
    65521 gtttttttat gaaagcagga acctgctagc atgtgtaatt aattatttct tcaggatagg
    65581 ataggaaata aaaaaagtcc cttggcatat aagccaaggg acgaaatctc gcggtaccac
    65641 ccaatttgta tgtctagata tccaacatac ctctcgaaca tttaacgctg tcgcgtggca
    65701 atcactgcca aagactcctg aaatgtaatt cgctttaaag catgtactgg tttgcagcga
    65761 ccaccagctt tctgaaacat tggctttaac aactacttcg tttcgatctt cgtcttgtta
    65821 ttattaatat gtgtctatca tagagcgcaa ctttttcttt gtcaacagaa aaaggatatt
    65881 tttttaaatt cctcattttt tcttttttga atccgctcta attccgaaca ttttattttc
    65941 aattattgtt aaaagaaata actttttctg tattttttct gcaatttatc gagaagtcac
    66001 tttactttgc ctcttattct ttcttcgtta caataaggaa tgggacaagt aaaaaaatga
    66061 aaaagcaagg gacgaggtat atggaaaaaa agcaaaaaat tattttagga gccacttctg
    66121 ctttaactat cgttatttta attctaagtt tgttttttgt catccgatca tctacaaaaa
    66181 aagaaccaat cattcctgaa aaaaaaataa cagaaaaagc aaaagaaccg acagatgata
    66241 agcaacaact agttactcaa gcaaaaaatc tggctcaaag ctacgattat gaccaagcga
    66301 ttgcgttatt ggaaaaagat agtaagaacg gatctactga aacacaacag cttcttcaaa
    66361 cactaaaaaa ggagaaagaa caactgatcg actggtctga tcctactcag atctctcatg
    66421 tctttttcca cagcttgatc gtggacccgc aaaaagcctt ccactctcaa caggcacaag
    66481 gatacaaaga ttatatggtg actgttgaag aattcaaccg ttcgatcgaa caactttatc
    66541 aaaatgattt tgttttagtt aatttgaatg atttgatcca aaaagacgaa caaggaaatc
    66601 tttcctttaa cgggcttgcg ctacctgaag gaaaaaaaac attggttctt tcacaagatg
    66661 atgtcagcta ttatgaatat atggataatt ccggttttcc tagccgattg gtcgtatcaa
    66721 ataacaatga aatcaaaaat gtctataaag agaaagataa agaagaaatc ggtgactttg
    66781 acatggttcc tctgatcgat gcctttgtca agaaacatcc tgatttttct tatcgaggcg
    66841 caaaaggtac cttggccctg acaggttaca acggagtttt aggttatcga acatcaaaat
    66901 ctcaatatgg agacaatgaa aaaacaaaaa aagagattaa agaagctaaa aaagtggcgg
    66961 aacagttgaa gaaagacggc tggagctttg cttcccatac atggggtcac ttgaatatga
    67021 ctcaagcttc actagaagat gtaaaaaaag acaatgaatt gtggcagcaa gaagttgcac
    67081 ctatcctagg aaaaacaaat attctgattt atccatttgg tgcagacatc agcgactggc
    67141 agccttattc agcagaaaat caaaaatttg cttacttgaa gagtcaaggt ttcgatattt
    67201 tctgtaatgt cgatgcttct acaccagctt ggggacaatt aggtacggat ttctatcgaa
    67261 atgctcggat caatatcgat ggcattcgtt ttcaatctga tttagacgga aaaaatccta
    67321 ttcttgatcc attcatcaat gtccatgatg tctatgacaa agaagcacgc gaatctgctt
    67381 gattttacgt catttatgaa ccaaaaaagc cgaactgaca ttttatcgtc agtttcggct
    67441 tttttatgct ttatttcttc ttcggtgcga ttcccttgat caggacttct gtccattctg
    67501 ctgtatatgg aagcagtttt gcgtatatct ccggcttaga aattccttct accattgctt
    67561 gaaaatagag gatcaagaac tcatccgaat atttcaaatc cacttttcct tctttccgcc
    67621 ctcgatggaa aagatcaagc atgatactga aactttcttg cgtatacgca tccatcgcgt
    67681 cagaaaattc ttttttttct ttttttgtat agtatgtcat caaatcgata taaaattgtt
    67741 tgttcactcg attgagataa cggattttgt tttcactcat ggcaatcagt gtttcttcaa
    67801 atggaagatt ttccgcaatc aacgctttcg cttcttgccc taaatcattc ataaatgcaa
    67861 aaaatacttc ataaatcagc ccttccttgc tgccaaaata tttaaaaatc gtcgctttac
    67921 ttacctgagc gttctcagct accttatcca ttgtcactcc agcggttcct atgtctgtgt
    67981 tcatcaagtc aaaagctgct tgtaaaatct ctttcttttt ctcttgtgct ctcttttcaa
    68041 aaccgttcat ttgtagagcc tcccgtaatt ttaaactttt ataatatata tagttcaaat
    68101 ttattctgag gtttattata actat
//
LOCUS       DACESX010000003        48455 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.003, whole genome shotgun sequence.
ACCESSION   DACESX010000003 DACESX010000000
VERSION     DACESX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.003"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     regulatory      145..242
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            358..939
                     /locus_tag="IXF88_000158"
     CDS             358..939
                     /locus_tag="IXF88_000158"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1506919.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            942..2249
                     /locus_tag="IXF88_000159"
     CDS             942..2249
                     /locus_tag="IXF88_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1506920.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2665..3153
                     /gene="purE"
                     /locus_tag="IXF88_000160"
     CDS             2665..3153
                     /gene="purE"
                     /locus_tag="IXF88_000160"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1506921.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            3137..4270
                     /gene="purK"
                     /locus_tag="IXF88_000161"
     CDS             3137..4270
                     /gene="purK"
                     /locus_tag="IXF88_000161"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1506922.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4283..5578
                     /gene="purB"
                     /locus_tag="IXF88_000162"
     CDS             4283..5578
                     /gene="purB"
                     /locus_tag="IXF88_000162"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1506923.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5758..6135)
                     /locus_tag="IXF88_000163"
     CDS             complement(5758..6135)
                     /locus_tag="IXF88_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506924.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            6391..7119
                     /locus_tag="IXF88_000164"
     CDS             6391..7119
                     /locus_tag="IXF88_000164"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1506925.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            7119..7373
                     /gene="purS"
                     /locus_tag="IXF88_000165"
     CDS             7119..7373
                     /gene="purS"
                     /locus_tag="IXF88_000165"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1506926.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            7375..8046
                     /gene="purQ"
                     /locus_tag="IXF88_000166"
     CDS             7375..8046
                     /gene="purQ"
                     /locus_tag="IXF88_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1506927.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            8047..10269
                     /gene="purL"
                     /locus_tag="IXF88_000167"
     CDS             8047..10269
                     /gene="purL"
                     /locus_tag="IXF88_000167"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1506928.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            10254..11693
                     /locus_tag="IXF88_000168"
     CDS             10254..11693
                     /locus_tag="IXF88_000168"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1506929.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            11725..12768
                     /locus_tag="IXF88_000169"
     CDS             11725..12768
                     /locus_tag="IXF88_000169"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1506930.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            12765..13343
                     /gene="purN"
                     /locus_tag="IXF88_000170"
     CDS             12765..13343
                     /gene="purN"
                     /locus_tag="IXF88_000170"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1506931.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            13340..14875
                     /gene="purH"
                     /locus_tag="IXF88_000171"
     CDS             13340..14875
                     /gene="purH"
                     /locus_tag="IXF88_000171"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1506932.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            14891..16165
                     /gene="purD"
                     /locus_tag="IXF88_000172"
     CDS             14891..16165
                     /gene="purD"
                     /locus_tag="IXF88_000172"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1506933.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            16287..17201
                     /locus_tag="IXF88_000173"
     CDS             16287..17201
                     /locus_tag="IXF88_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1506934.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(17334..18899)
                     /locus_tag="IXF88_000174"
     CDS             complement(17334..18899)
                     /locus_tag="IXF88_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1506935.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(18892..20343)
                     /locus_tag="IXF88_000175"
     CDS             complement(18892..20343)
                     /locus_tag="IXF88_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1506936.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(20357..21373)
                     /locus_tag="IXF88_000176"
     CDS             complement(20357..21373)
                     /locus_tag="IXF88_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1506937.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            21593..22681
                     /locus_tag="IXF88_000177"
     CDS             21593..22681
                     /locus_tag="IXF88_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1506938.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            22735..24261
                     /locus_tag="IXF88_000178"
     CDS             22735..24261
                     /locus_tag="IXF88_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1506939.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            24263..25468
                     /locus_tag="IXF88_000179"
     CDS             24263..25468
                     /locus_tag="IXF88_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1506940.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            25725..26702
                     /gene="guaC"
                     /locus_tag="IXF88_000180"
     CDS             25725..26702
                     /gene="guaC"
                     /locus_tag="IXF88_000180"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1506941.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            26966..28390
                     /locus_tag="IXF88_000181"
     CDS             26966..28390
                     /locus_tag="IXF88_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1506942.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            28851..29960
                     /gene="pdhA"
                     /locus_tag="IXF88_000182"
     CDS             28851..29960
                     /gene="pdhA"
                     /locus_tag="IXF88_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1506943.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            29963..30940
                     /locus_tag="IXF88_000183"
     CDS             29963..30940
                     /locus_tag="IXF88_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1506944.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            30965..32608
                     /locus_tag="IXF88_000184"
     CDS             30965..32608
                     /locus_tag="IXF88_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAQ1506945.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            32616..34022
                     /gene="lpdA"
                     /locus_tag="IXF88_000185"
     CDS             32616..34022
                     /gene="lpdA"
                     /locus_tag="IXF88_000185"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1506946.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            34184..34459
                     /locus_tag="IXF88_000186"
     CDS             34184..34459
                     /locus_tag="IXF88_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1506947.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            34465..35232
                     /locus_tag="IXF88_000187"
     CDS             34465..35232
                     /locus_tag="IXF88_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1506948.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            35400..37229
                     /gene="typA"
                     /locus_tag="IXF88_000188"
     CDS             35400..37229
                     /gene="typA"
                     /locus_tag="IXF88_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1506949.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            37490..39262
                     /gene="recQ"
                     /locus_tag="IXF88_000189"
     CDS             37490..39262
                     /gene="recQ"
                     /locus_tag="IXF88_000189"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1506950.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLRKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            39376..39678
                     /locus_tag="IXF88_000190"
     CDS             39376..39678
                     /locus_tag="IXF88_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1506951.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            39681..40844
                     /locus_tag="IXF88_000191"
     CDS             39681..40844
                     /locus_tag="IXF88_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1506952.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            40870..44298
                     /locus_tag="IXF88_000192"
     CDS             40870..44298
                     /locus_tag="IXF88_000192"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1506953.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            44319..45443
                     /locus_tag="IXF88_000193"
     CDS             44319..45443
                     /locus_tag="IXF88_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506954.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            45433..45870
                     /locus_tag="IXF88_000194"
     CDS             45433..45870
                     /locus_tag="IXF88_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1506955.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            45854..46192
                     /locus_tag="IXF88_000195"
     CDS             45854..46192
                     /locus_tag="IXF88_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1506956.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            46311..46871
                     /gene="rsmD"
                     /locus_tag="IXF88_000196"
     CDS             46311..46871
                     /gene="rsmD"
                     /locus_tag="IXF88_000196"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1506957.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            46868..47359
                     /gene="coaD"
                     /locus_tag="IXF88_000197"
     CDS             46868..47359
                     /gene="coaD"
                     /locus_tag="IXF88_000197"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1506958.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            47349..48392
                     /locus_tag="IXF88_000198"
     CDS             47349..48392
                     /locus_tag="IXF88_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1506959.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT        15883 a         8305 c        10868 g        13399 t
ORIGIN      
        1 tttacaaaaa aataagtaaa ccacgttgtc agtttcagaa agtagtgtta gactgttttc
       61 aatatttgaa acaacatttt ttattcaaat atggcaaact gccttcacgt gcaaaacagt
      121 tgcaagaaaa tgaatttaca ctattattag ttttacatac ctacttatat atcgtcataa
      181 tatggatgac agtttctagc cagtaccgta aatgctggac tataagtaaa agattggcta
      241 tttcaccctt cttttcttat gataaagtgg agggtgtttt tctttaacag aaacatagta
      301 ggtttttgca caaaagcggt tatttcggta ttaataaatg ataaaggagc aaataaagtg
      361 aaagaattag ttgaacgtat tcaaaaagac ggacgtatat taggcgaggg cgtattaaaa
      421 gtcgacagct tcgtcactca ccaagtagac cctgtattga tggaacaaat gggaaaacgg
      481 tttgcagaag tatttaaaga acaaggaatc acgaaagttg tgaccattga agcttctggg
      541 atcgcaccag ctttatttgc tgcaagagaa ttagatgttc caatgatctt cgctagaaaa
      601 gcaaaaagtt tgacgatgga tgaagaatta ttgacagctt ctgtttattc ttttacgaaa
      661 caagtgacaa gtacgatttc tatttctcgt aaatttttat cttcagaaga caaagtattg
      721 attatcgacg atttcttggc aaacggacaa gcggcaaaag gattgatcga attatgccag
      781 caagctggag cgcaagtaga aggaatcggg atcgtcatcg aaaagtcttt ccaagatgga
      841 cgtcagttac ttgaagaaat gggattgaga gtcgtttctc ttgcgcgtat tgcttccttg
      901 acgaatggac aagttgagtt tcttgaggag gacgcataat cgtggaaaaa gaaacacaaa
      961 atggaaaagc tgcggtttta gggctgcagc atctgctagc gatgtatgca ggagcagtag
     1021 cggtgcctct tttgatcggt acaggactag gctttaatca agaacagatg acttatttga
     1081 tttcaattga tatctttatg tgtggattag cgacattatt gcaattgaca gttaaccgat
     1141 ttttcggaat tggcttgcct gtcgttttag gatgtgcgat ccaagcagta gctccactga
     1201 tcttgatcgg aaccaatgaa ggagtaggag ctatctacgg atctattatc gcttctggga
     1261 tctttgttgt gttagtttct ggtgtctttt ctaaaattaa aaaactattc cctcctatag
     1321 taacaggaac ggttatcacc gttattggtt tgactttgat tcctgtagca atcgagaaaa
     1381 tgggtggagg taatgcaaca gcagctgatt ttggtgacaa aacaaatctg ctacttgcat
     1441 ttgtgacgat tctcctaatt gtaggcgtgc aaatgctggc aaaagggttc attcgttcaa
     1501 tcgcggtatt gattggttta gtcggaggaa gcatcttagc tgctttcatg ggaatgattg
     1561 atctgagcgc gattggagat gcgccgttgt tccatgttcc acaacccttt tattttggta
     1621 aaccaacttt cgacgtatgg tcgattttac taatgatcat catttccatg gtcagcatgg
     1681 tagaatcaac aggtgtatat tttgcacttg gtgacattac tgggaaaaat gttggagaag
     1741 aagaattgaa aaagggatat cgtgcagaag gacttgccgt tatcttaggc ggtattttca
     1801 atacattccc ttatactggc ttttctcaaa atgtcggatt ggttcaactt tcgggaatca
     1861 aaacaagaaa acccatctat ttctctgcat tcttcttgat tgttttagga ttattcccta
     1921 aaatcggtgc gttagcacaa atcatccctg agccggtact tggtggagga atgttagtca
     1981 tgttcgggat ggtggcagtc caaggaatcc gtatgctgtt ggaagttgat ttcagcaatg
     2041 ataaaaatct attgattgtt gctgtttctg ttggatttgg gctgggtttc aatgttacgc
     2101 cgacattatt cagtcaaatg ccgcaaacat tgcaaatgtt cactggaaat ggcatcgtaa
     2161 tgagtagcat tacggctatc atattgaatt tgatctttaa tggattgaaa aaagatgaag
     2221 aaatattgga agaagccttc aagaagtaat agagcaaaag aaaggttgtg aaatcagcgt
     2281 tttgacctga ttgttagaga aacaaacgga aaaatagttc ctcgtatttt gccgtttgtt
     2341 tgagctaata ccgagggtca gctgattgaa caccgttgaa ggaagaggtc gtgagcctgc
     2401 cgttcatctc cgagtcacaa ggaacaattt caaaaaaaag cttctcatgt ttgttgaata
     2461 ttgttccttg tgaccgagga gttggcagga gaacaccgtt tatccataaa aaggaatgtg
     2521 acctaacttt tgtcacattc ctttttttta tctttaataa acacgaacaa taaaatgttt
     2581 ttctttgtta acattcgaaa tttctttgac atcaaaaaaa gtgattgtta aaatatgaat
     2641 gtagaaaaac tgggaggaaa aacaatgaca gcaaaaatct ctgtgattat ggggagtact
     2701 tcagattggg aaacaatgaa agaagcatgt caaatattgg atgaattcgg tgtgtcttat
     2761 gaaaaaaagg tcgtttctgc tcatcgaacc ccagatttga tgtttgaata tgcacaaaac
     2821 gctcgaaaaa aaggaatcaa aatcattatt gcaggagcag ggggagccgc gcatctgccg
     2881 ggtatggtcg catcaaaaac aactttgccg gttatcggtg taccggtcca atcacgaaca
     2941 ttaaatggtt tggattcgct tctttcgatt gtccaaatgc ctggaggagt acctgtagct
     3001 actacagcaa tcggaaaagc gggagcaatt aatgctggac tacttgcgat acagatgctt
     3061 tccatgtatg atttagaatt ggaggctaaa ttggctgaaa gacgttcaat gttggctaaa
     3121 actgttatag aaagtagtga tcaacttggt taaacctttg ttgcctggtt caacgatcgg
     3181 gatcgtcggt ggcggtcaat taggtcgtat gatggcgatt agtgcaaaag aaatgggctt
     3241 taaagtaggt gtgctggatc ctgtgagcga ttgtccagcc gcacaagttg cagattggca
     3301 tattgaaagt gtctatgatg acacctttgc gctgaaagag cttgccagaa gaacagatgt
     3361 cattacctat gaattcgaaa acgtcagtgt agaggcgtta aatgcgattt tacccatgtc
     3421 atttattcct caagggacag acttgcttgc gattacgcaa gaccgcctgt tagaaaaatc
     3481 ttttttggaa acgaataaca tcgtgattgc tccttatgca acgatcgtca gtccgacgga
     3541 tatccaagat gcgattgacg ggatcggcta tccttgtgtt ttgaaaacca ctcgaggcgg
     3601 ctatgatggc aaggggcagt atgttttgaa aagtcgggcg gatctagctc ctgccatgga
     3661 ccttttaaga gaaggaacct gtgttttaga agcatggatc ccgtttgaaa aagagatttc
     3721 gatcatggtg gcaggaaatg gtcaaggaga tttcacgact tttccggtcg tggaaaacat
     3781 ccaccacaac aatattcttc atgaaacgat tgctccagcc gcgattgatc aggatgtgat
     3841 cgaggaggct gagcgtattg cccgtgtgat tgcagaagca gtgagccttt caggtgtttt
     3901 aggggtagaa atgtttttaa caaaaacagg cgggctatat gtgaatgaat tagctcctcg
     3961 tcctcacaat tctggacatt attctattga agcttgttct atgagtcagt tcgatgccca
     4021 tattcgaggt atttgtggtt ggccattagg agaaaaagta cgtttgataa gcaatgcagt
     4081 aatggtcaat gttttgggag aacaactgct ggaaagctat cgcttgattg agaataagcc
     4141 agactggcaa ttccatttct atggaaaaaa acaagcaaaa aaaggtcgca agatgggaca
     4201 tatcactatc ttgacagaag acatccagca aacgctggaa gaaatcaaac aaacgaatat
     4261 ttgggattag aggagaaaaa acatgttaga acgttataca cgccctgaaa tggggaatat
     4321 ctggacggaa gagaacaaat accgtgcgtg gctcgaagtc gagattttag cagatgaagc
     4381 ttgggcagaa cttggtgaaa tcccgcaaga agatgtgaaa aaaattcgtg agcatgcatc
     4441 atttgatatc gatcggatct tggaaatcga aaaattaaca cgccatgatg tcgttgcgtt
     4501 tacacgagcg gtttctgaaa cattgggaga agaaagaaaa tgggtccatt atggtctaac
     4561 aagtacggat gtcgtagata ctgcttatgg ctatctcatc aagcaagcaa atgatatttt
     4621 acgggaagac ttgaaacgtt ttacggaaat cattggcgaa aaagcaaaag aacacaaata
     4681 tacagtgatg atgggtcgga cacacggtgt ccatgctgaa ccaactactt ttggattgaa
     4741 acttgccaca tggtattcag aaatgaaacg gaacatcgaa cgatttgaac atgcggcaaa
     4801 aggagtagaa gcaggaaaaa tctcaggagc agtgggaacg ttcgcaaata tcccgccatt
     4861 tattgaagag tatgtatgta aacaattggg gatccgtcca caagaaattt ctactcaagt
     4921 tctccctcgg gatctacatg ctgaatattt ctcagcgatg gcgcttattg ctacaagtat
     4981 tgaacgtttt gcaacagaga tacgcggatt gcaaaaatcc gaaacacgtg aagtagaaga
     5041 attttttgcg aaaggtcaaa aaggttcttc tgctatgccg cacaaaagga atccgatcgg
     5101 ttcagaaaat atggctggtc tagccagagt ggtgcgcgga cacatggtca ctgcctatga
     5161 aaatgtcaca ttatggcatg aaagagatat ttctcattct tcagcagaac gaatcatctt
     5221 gcctgatacc acgattttat tgaattacat gttaaatcgt tttggcaata tcgttaaaaa
     5281 tctaacggtc tttcctgaaa acatgaagcg gaatatgtct gcaacatttg gtttgattta
     5341 tagccagcgc gttatgctga aattgatcga taagggaatg acaagagaag aagcttatga
     5401 tcttgttcag ccaaaaactg cgtacgcttg ggacaaccaa gtgcagttcc gcccgctgtt
     5461 agaagaagat ccaaccatca catcggtatt atctaaagaa gaaatagacg atgcctttga
     5521 ttatcatcat catttgaaaa acgttgacgt gatattcaaa cgagtaggtt tggaataaag
     5581 aagagtgagt tgcaaaattc aattaaaata gaatagcaaa aataaccaaa aaagaagcag
     5641 atccgttagc ggttctgctt cttttagttt attatatttt cttatcaata ataggaacct
     5701 gattttaaaa gcggctacct ctttaccgtg cctctttaca tacaaaaaat agaggaatta
     5761 agcgtaaatg gcttggctat tataactaaa atgctggtta tggagatgtt gcagcaagtt
     5821 ttcgagaaaa gcgtattcca tctcttgtcc attgaataag atctggatac gataatgatt
     5881 tttgaaacgc ttctgttcat gaatctgact gattgaaagt tcgcctttgt gttctgtctg
     5941 ataaagaaaa gtccttttac ggcattcagc acagcgcttg gttgctcggc ggatttttcg
     6001 gaatgtataa aaactagtat ctttattgca taaataaacg tctttgatcg acagcaaatc
     6061 attatataga caggaacgtt cttcagctgt taattctgat aaaaaaagct gaccttgttt
     6121 tattgcatct tccatcggca agtgcctcct cgaacccatt aaccaaattt ttagttaatt
     6181 ctaatatacc atcattaagt ataaatgtaa acgcttgttg atggtataag attttcgttt
     6241 ctgataaaac acgaacatta aaagaatcta atgccttaaa tgtacatatt tttgttgatt
     6301 tataaaaaga tgattgctaa aatttaaagt gaaaaaacga acaaaaaacg atgtatttgc
     6361 ttttaatgta aataaaaagg agagacgtac atggagaaga aagaactttt gtatgaagga
     6421 aaagccaaaa aaatatatct tacttctgat gagaacttga tttggataga atatttagat
     6481 caagcaactg cgttgaatgg cgcgaggaaa gaccagataa gtggaaaagg agaattgaat
     6541 aatcaaatca caagtgcaat tttccgttat ctgactcaaa gaggaataaa aaatcatttt
     6601 attgaacaat tgtctaaaac cgaacaatta gcaaaaaaag tacaaattat ccctctggaa
     6661 gtggttatcc gaaatattgc agcaggaagt ttctcaaaac gtctaggtgt tcccgaaggt
     6721 aaagaattca tcaaaccaat cattgaattc tactataagg atgaccagtt ggatgatcca
     6781 tttatcaatg aagagcatat ctatttctta caacttgcaa agccggaaga aatcagtgag
     6841 ctgaaagccc aagcgttggc tattaatgaa attttgaccg aattatttgc taaggtcggt
     6901 ttggttttag tagatttcaa gctagaattt ggccggtcga atggacaaat tttgcttgcg
     6961 gatgaagtaa caccggatac ttgccgatta tgggatgaac gtacgaatga acatatggac
     7021 aaagacgttt accgcagaga tctaggtgat ttgatcccag tctatcaaga agtatataac
     7081 cgtctatcgg aactaactga agaaaaggag cgagtatgat gtatttcgta aaagtatatg
     7141 taacctataa agattcagtg ttagatcctc aaggggaggc agtaaaaaaa gcagtccatc
     7201 gtatgggata tgaaacaatc gaagatatcc gaataggaaa atactttgag atacaacttg
     7261 ctgatgcaga agaaccaatc gatacagtga ttgaaacgat ttgtgacaag ctgcttgcaa
     7321 atgtaaatat ggaaacatac cgatatgaaa tcaaaaaaat ggaggaagta taacatgaaa
     7381 tttgcggtca tcgtttttcc aggttcaaat tgtgacgcag atatgttatg ggcagttcgc
     7441 gagatcatgg gcgctgatgc cgaatttgta cggcatgatg agtcctcttt agaaggattt
     7501 gatggggttt tactgccggg aggattctcc tatggtgact atttgagatg cggtgcgatt
     7561 gctcggtttt ctacaatcat ggaagaagtg attcgttttg ctgaagaagg gaaaccagta
     7621 ttcggtacat gcaatggatt ccagatattg acagaagcag gacttttgcc aggtgtactg
     7681 agacgaaatg agtctttgca ttttatttgc aaaactgtac cattgaaagt caaccccaag
     7741 acggccttta cttcagagta tgaagccgac gaaataatct atcttccagt tgctcatggg
     7801 gaaggaaatt atttctgtga cgaagaaaca cttcaagaac taaaaaaaaa taatcagatt
     7861 gcctttactt ataatgacaa catcaacggc agcgtggaaa acattgcagg gattacgaac
     7921 aaagcaggga atgtgttagg aatgatgcct catccagaac gtgcagtaga agacttgcta
     7981 ggatcagaag atgggaaacg gttctttgct tccattttaa aaaacttcgg aaaggtgacc
     8041 gtatagatga aaacattgaa agaaccgaca gctgaagaga tcaagaaaaa aagaatctat
     8101 gccgaatggg gattgactga tgaagaatat cgaatgatcg aagaagatat cttaggaaga
     8161 atgcctaatt atacggaaac tggactgttt tctgttatgt ggagcgaaca ttgttcctat
     8221 aaaaattcta aaccggttct acgcaaattt cctactagtg gacctcatgt gttgcaaggc
     8281 cctggagaag gcgcaggcat cgtcgacatc ggtgatggac tagcagttgt cttcaaagca
     8341 gaaagtcaca atcatccttc agccgtggag ccttatgaag gagctgcgac aggagttggc
     8401 ggaatcatca gagatatttt cagcatgggg gctcgtccaa ttgcgttgct agattcattg
     8461 aggttcggcg aattagataa tccaagaacg aaatatttac tagaagaagt agttgcaggt
     8521 atcggcggat acggcaattg tatcggcata ccgactgtag gcggcgaaat tgcatttgat
     8581 ccttgttatg aaggaaaccc attagtcaat gcaatgtgcg taggtttgat tgaacacaaa
     8641 catatccaga aagggcaagc cgctggcgtc ggtaattcga tcatgtatgt aggtgcaaaa
     8701 acaggtcgcg acggtatcca cggagcaact tttgcctcag aagaatttaa tcaagaagaa
     8761 gaacagcaac gttctgctgt acaagtaggc gatcctttta tggaaaagct gctgcttgaa
     8821 gcgtgtttgg aattaatctt gcatcattcg gattctctaa taggtataca ggatatggga
     8881 gcagcaggac ttgtttcatc tagtgcggaa atggcttcaa aagctggctc aggacttata
     8941 ttgacattag atgaagtccc acaaagagaa actgggatga caccttatga aatgatgtta
     9001 tcggaatcac aagaacggat gttgatttgt gttaaatcag gagaggaaga aaaaatccaa
     9061 gaactatttc agaagtatga tctagatgca gtcactatcg gtaaagtaac agacgacggg
     9121 caatatcggc tatatcatca aggagaagaa gtagcaaatc tgccagtcga tgcattagct
     9181 gaagatgcac cagtttatca taaagaaatg aaagaaccag ctcgaatcgt agaatttcag
     9241 cagttagctc cttatcagcc agttatcgaa gaacctggac aagtattgct tgatctgctg
     9301 cagcaaccga cgattgcttc aaaacgttca atctacgaaa cttatgattc acaagtacaa
     9361 accaatacag ttgtccgtcc aggaagcgat gctgctgtga tgagggttag agggacgaat
     9421 aaagcgttag ctatgaccac agactgcaac gcgcgctatc tgtatttgga tccaaaaatc
     9481 ggaggacaaa tagcagtggc agaagctgct agaaatatta ttgctagcgg tggtaagccc
     9541 ttggcgatca ccgactgcct gaactatggt tctcctgata aaccagaagt tttttgggaa
     9601 ttatcgactt cagctgacgg aattgctgct gcgtgtgaaa cgttaggaac tcctgttatt
     9661 tcaggaaatg tctctttata taatgaaacg gatggtcaag caatctatcc aacaccaatg
     9721 attggtatgg tcggattgat tgaggatcat aaacatatca ctactcagga attcaaaaaa
     9781 tcaggggatc taatctatat attaggtaag acgtttgcag attttgatgg cagtgaatta
     9841 caaaagatgc agcttggaag aatcgaaggt gtgatccgaa actttgatct atccattgaa
     9901 aagagaaacc aagagttagt attgacagcc atccaaaacg gattgattga aagtgctcat
     9961 gactgttcag aaggtggctt ggcaattgca ctggcagaat cagctttcaa acatcagcta
    10021 gggatatctg tacagtttga attgtcctct gctcaattat ttgcagaaac tcaatcaaga
    10081 tttgtgttga cagtcgcacc ggaaaataag acacgattcg aagaaatgat gggagatgct
    10141 gctgttttag caggtaaggt gacggatgaa gcgataatcg aaatttcagc aactgacggt
    10201 cagatcaaaa tagaaactgc agtagcaaga aagtgctggg aggatgcaat cgtatgtctt
    10261 atgaagtgaa gagtttaaat gaagaatgcg ggatcttcgg tgtttggggt catccagacg
    10321 ccgctagtgt ggcatacttc gggctccata gtcttcagca tcgaggacaa gaaggtgcag
    10381 gtatcgtgtc caatgatcga ggaaagctta aggggcatcg agatttgggg ctgctttctg
    10441 aggtattcaa agatcaaaga aaattgcagc agttaacagg agaagctgca atcggacatg
    10501 tacgttatgc aacagcaggc aatggaagtg tggacaatat tcagcctttt ctattcaaat
    10561 ttcatgatca gtcgatcggc ttagcgcata atggtaactt gatcaatgct cgttccctta
    10621 gaaaagagtt agaggaaaat ggggcgatct tccattctaa ctccgatacg gagatattga
    10681 tgcatttgat ccgacgaagt gaagaaacag attttttaaa taaattaaaa agtgctttga
    10741 atgaagtcaa aggtggcttt gcctatttat taatgacaga aacggcaatg attgccgctt
    10801 tggacccaaa tggttttcgt ccattagcaa tcggacaaat gaaaaacgga gcatacgtga
    10861 ttgcctcgga aacttgtgca ttagaagtta ttggggcacg ttttataaga gatgtccagc
    10921 ctggtgagat agtgatcatc aatgacgaag gaatccagat tgattgcttt acgaaggaca
    10981 cgcagctttc tatttgttcg atggaatata tctattttgc cagaccagat tcaaacatcg
    11041 cagggatcaa tgtgcatact gcacggaaaa atatgggacg gaatttagcg aaagaatcgc
    11101 cagtaaaagc agatatggtc gttggcgtac ctaattcttc tttgtcagcc gcaagcggtt
    11161 atgcggaagc tagtggaatc ccttatgaaa tcggattagt aaaaaatcaa tacattgccc
    11221 gaacatttat ccagccaacg cctgagctaa gagagcaagg tgttcgaatg aaattatctg
    11281 ctgtacgagg agtagtggaa ggtaaaaagg tgattatggt cgatgattca attgttcggg
    11341 gtacgacaag cagacggatc gtccatcttt tgaaagaagc aggggcaaaa gaagtccatg
    11401 tgaggattgc ttctcctcct ttgaaatatc catgttttta cgggattgat attcaaacgc
    11461 gtaaagaact cattgcggca aatcacacga ttgatgaaat caaagaatgt atcggtgcag
    11521 attctttagg ttttttaagt gaagagggtc tgatcgaatc gatcggtctg gaaaaagatg
    11581 ctccgtattc aggattgtgt atggcttatt tcaacggaga ttatccgacg ccgttatatg
    11641 actatgaaga aaaatatcag gaatcactaa aagaaaaagt atcgttcttt taaaagcaga
    11701 ccaagaaggg agtatatgaa ttcaatggca aatgcatacg caaaagctgg cgtagatgtc
    11761 gaagctggat atgaagtagt agaacggatc aagaagcatg tcaaaaaaac ggaacgttta
    11821 ggtatgatgg gcgctatagg aggattcggc ggttgttttg atttatcggt ctttcaatta
    11881 aaagagccag tccttgtctc ggggaccgat ggcgtgggga cgaaattagc agtggcaatc
    11941 gaagcagaca aacacgatac aatcgggatc gactgcgtag cgatgtgtgt caatgatatc
    12001 ttagcacaag gcgcagagcc gttatatttc ttagactact tagcaacggg gaaaaatgat
    12061 ccgttacgtt tggaaaatgt agtaaaaggt gtagcagatg gatgtgttca aggaaatatg
    12121 gcgctgattg gcggagaaac agctgaaatg cctggtatgt actcaggtaa tgattatgat
    12181 ctagccggat ttgctgtagg gatagctgaa aaatctcaat tattgacagg agaaacgatc
    12241 aacccaggag atgtgttgct tggaatagca tctagcggga ttcattcaaa tgggtactct
    12301 cttgttcgca aaattttctt tgagcagcag cagttttctt tatcatcaaa actgccagaa
    12361 ttgaaaaaaa cacttggcga agaattgctt gagccaacga aaatctatgt ttcttccttg
    12421 cttccgctga tcaaagaaaa gaaaataaaa ggagctgcac atatcactgg tggagggttt
    12481 gtagaaaata tcccgcgaat gttaccagaa tctatggctg cacaaatcaa acttggctca
    12541 tggccgattc ttccaatatt cggctgttta caaaaatatg gtgaaattcc tgagatggag
    12601 atgtatgaaa tcttcaatat gggcatcggt atggtcctgg cagtcgaaga atcgaaagta
    12661 aaagaaatag tacacgaaat tgagcaagct ggcgaaaaag tttatgaaat cgggaaagtg
    12721 atcgccaaag aaaaacatcg tgtggtgttt ataggagaag aacgatgaga atcgctgttt
    12781 ttgcttctgg aaacggaagc aactttcaag cgctagccga ttatctatcg aaaaaaggat
    12841 tggaatcttc gattgactgg ttgttttgtg atcagcctga agcatatgtt ttgaaacgtg
    12901 caactgcgtt gagtgtaccg gcagattgtt tttcacctaa agaatttgat tcaaaaaaag
    12961 agtatgaaga agcaattttg cataaattga aagaaaaaaa gatcgatttg atcgtattag
    13021 ctggatatat gcgaatcatt ggaccggtat tgctgaaaaa ttatgataaa agaatcatca
    13081 atatccatcc gtctctttta cctgccttcc ctggtttgca tggcatacgt gatgcctttg
    13141 aagctggagt aaaagaaacg ggtgtcacaa ttcattatat cgaccaaggt gtagatactg
    13201 gaccaatcat ccggcaggaa aaagtgagaa ttgaacagga ggacacgttc gattctttag
    13261 aagaaaaaat ccatagagta gagcatcgaa tctatccaga agtgattagt gaaattattg
    13321 aaaatggagg ctatctgaaa tgacaagagc attgatcagt gtatcagata aaactgggat
    13381 tgtagcattc gcccaaggtt tgattgaaca aggagtagaa atcatttcaa caggaggaac
    13441 aaagcatgcg ttggataaag ctgggatcaa aacgctttcg atcgaagaag tgacagggtt
    13501 tcctgaaatg atggatggcc gtgtcaaaac acttcatcct aaaatccatg gtggtctttt
    13561 aggaagacgc gatctcccta gccatatgga ggcaatggaa acggaaaata tccaaccaat
    13621 cgactttgtt tgtgtcaatt tatatccttt caaagaaacg attatgaaac cagatgtcga
    13681 aatagcagaa gctattgaaa acattgatat cggtggtcct agtatgctcc gtagtgcagc
    13741 aaaaaaccat gaatttgtca cctctgttgt tgatccatct gactatgaag aagtactttc
    13801 agaattacgt cagtatgggc atacttcact ttctttcaga caaaaactag cggcaaaagt
    13861 ctttcgtcat actgcggcat atgatgcact gatcgctcaa tacttaacgg attgggtgga
    13921 agaaaaagaa ccggaaaaat tgacacttac gtatgaacga aaacaaacgc ttcgttacgg
    13981 tgaaaatagc catcaagccg ctgcctttta tcagtccgct cttctggaaa aatattcgat
    14041 cgcagcagca aaacaactcc atgggaaaga actttcttat aataatatcc gagatgcaga
    14101 tgctgcgcta cggatcatga aggaatatga cgagccaacg gtagtcgcct tgaaacacat
    14161 gaatccttgt ggtattggga cagcatctac gattttagaa gcgtgggaag cagcgtatga
    14221 agcagatcct gtttcaatct ttggtgggat catcgttttg aatcgagaag tagatctgcc
    14281 aacagcagaa cagatgcata agctgtttct agaaatcatc attgcccctt cttatacaca
    14341 agaagctttg gaattactgc aaaaaaagaa aaacatccga ttactgacag tagactttgc
    14401 gcaggaagaa aatcaaaatc gagaagaaac ggtctcagtc ttaggtggac ttcttgtaca
    14461 agatcaagat ctggcaatag aaaatgaaga agagtggaaa gtcgtgacaa aacgtaagcc
    14521 gactccagca gaagcaaaag cattagtgtt tgcttggaga gctgtcaaac atgtgaaatc
    14581 taatgcgatc gtactggcaa acgaacgtca gacagtagga atcggagccg ggcagatgaa
    14641 tcgagtcggt tcagtaaaaa ttgcgattga acaagcgcaa gagaaaatgg aaggtgcagt
    14701 tttagctagc gatgcttatt ttccaatgga tgattcggtt gaatatgcag ccaaacatgg
    14761 tataaaagca atcattcagc caggaggaag catcaaagac caagattcta ttgatatggc
    14821 gaataaatac gatctagcaa tggtctttac tgatgttcgg cattttagac attagagagg
    14881 agtcggataa atgaacattt tggtgatcgg tgcaggtggt cgtgaacatg caataagtaa
    14941 aaaactttta gccagtccga aggtggatac ggtgtattgt gcgccaggaa atccggggat
    15001 gacaaaagat ggtatcaatt gtgtcgctat cagtgagcga gatcattcag cgttaatcca
    15061 ttttgttaag aaagaggcta ttgattggag tatcgttggt cctgaagttc ctttgttaaa
    15121 tgggatcgtc gatgatttta tggcggaagg attgaaaatc ttcggtccga caaaagcagc
    15181 agctctgatc gaaggttcga aaagttttgc taaacaatta atggatactt atcaaatccc
    15241 gacagctaaa tccagaagtt ttgaatgtta cgaaaaagca gcagcgtata tcaaagaaaa
    15301 tggtgcgcca atcgtagtga aagcggacgg cttagcagct gggaaaggtg tagtcgtggc
    15361 taccacagtg aaggaagcgc tggatgcggc agaacaaatg ttgaaccagc atcgatttgg
    15421 ggatagtagc aaaaaaatcg tcgtagaaga gtttttggca ggagaagaat tctccctttt
    15481 ggcgtttgtc cgtaatacca aggtgtatcc gatggtcatt tcgcaagatc ataagcgggc
    15541 atttgataac gataaagggc caaatacagg cggaatgggt gcttattcac cagtaccgca
    15601 aatttcaaaa aagatgattg aaaaagctgt ggaagaagtt ttaaaaccag cggctttggg
    15661 aatgaaagaa ctaggacgct ctttcacggg tgttttatat gcgggtttga ttgctacgaa
    15721 ggaaggacca aaagtgatcg aattcaatgc gcgtttcggt gatcctgaaa cgcaagtcgt
    15781 tctttctcgg ttgaagagtg actttgcgca agtaatcgat gatttattag agaatcgtac
    15841 agtagagtta aagtggcagc aagaaggata caatgtaggc gttgtggttg cagctgccgg
    15901 ttaccccgaa gaatatgaga caggaatggt tctaccagat tttctggaag aagagctttc
    15961 tgtttactat gctggagttt cctcaaataa cggcaaacta gttggagcag gaggacgtct
    16021 ttatttagta gaagcatacg gtgagacgat caaagaagca cagaaaaaaa tttacaccgc
    16081 gttaatggat aaaacgacag atggaacatt ttatcgtaca gatattggag aaaaagctgc
    16141 caatagcgaa ctgatcgtga agtaaatcag ctgcttccat ttataaaaaa tacaacaaac
    16201 ataatcggaa gcgccgattt tagtgaagcc ttttcataaa gagtgtggta cacttttgtt
    16261 gtgtatataa ataaggagga gttcagatga aaaaaattca attaggtact tctgatcaga
    16321 aagcagctgc agtgatctta ggctgtatgc gtatcaacaa tgcggaagat ccagtaaaag
    16381 tgattgagac agcttatgaa aacggcattg atttttttga ccatgcagat atttatggaa
    16441 acggtgaatg tgaagaaatc tttgcagatg ctctagcaaa aacatcaatc aaacgagaag
    16501 atctttttat tcaaacaaaa tgtggaatcg ttccaggtgt gatgtttgat ttttctaaaa
    16561 aacatatcat tgaatctgta gaaaaaagtt tgacccgtct gaaaatggat tatgtggatg
    16621 ctttacttct tcatcgtcca gatacattgg tagaaccaga ggaagtagct gaagcgtttg
    16681 atgaattgga acggtcagga aaagttcgtt attttggcgt cagcaatcaa aaaccaatgc
    16741 aaatcgaatt gctgaaaaaa tatgttcgtc aaccattagt agctaatcag ctgcaatttg
    16801 gcttgaaaca tactggaatg atcgatcaag ggattcatgt caatatgaca gatgaagcaa
    16861 gtatcgatca tgacggtagt gtacttgatt attcccgatt gcatgatatg acgatccaag
    16921 catggtcacc ataccagtat ggattttttg aaggtgtatt tatcgggaac gaaaaattcc
    16981 ctgaattaaa ccaaacatta gaagaactag cagctaaata tcaaacgaca ccaactggct
    17041 tggcttctgc ttggatttta cgccatccag ccaatatgca agtgatcgcc ggtacgatga
    17101 acgtagggcg aatcaaggaa atcgctcaag cttcagaaat taatatgtct cgggaagatt
    17161 ggtacgaatt gtaccgtgct gctggaaaca ttttacctta aagattgtca taagcaggtc
    17221 aaaagggcta taaattaaaa agacagaata acttgaaagc tgttttcagc ttcgagatat
    17281 tctgtctttt tttattgcaa atggaaagag tatcattgta cggatttcgt ttattagtta
    17341 tttttgtttt taaggaaaag ctgtgtccga tattcttttg gagaaatccc ttgttttttc
    17401 ttaaataaat aactgaaata gttagggtca ttataaccaa tttcaaaagc aatatctgaa
    17461 atacgatagt ctgattgctg caataatttt tttgctaatt cgattctttg ttctgtcaga
    17521 taatcgatga atgtttggtt aaacgattga gaaaaaatcg tactgaaatg agagggactt
    17581 aatgcaactt gttgagcgac ccaagttaag gacatatctg gatcggtaaa attttctttg
    17641 ataaaatcca atgcatggtt caaaattggc tgatatttgt tccttgaagg ctgtgagggc
    17701 agagcagtta agaaagaaat gaacatataa actgtcgaac ggtaaagcga gtcgtcggcg
    17761 ctgattgcta agcgttgact ggtggatgtg caatgaacca tttcttgcgg gattttcttt
    17821 tctttcaggc agacatgctg gagtagctta gtcaagacaa ataaacgata taatcgtgtt
    17881 cgagttggtg tgccttgtgg tttcgtcaag tgttcgatgt acttagatac ttcgtttttc
    17941 gataatgaag acaaatcaag tgagaggtca aagtaaaaga cagaatcaac tggaagtttc
    18001 gcttcttgaa tcaaatcctc atagctgata atttgttctt taagggaact ggcggtttca
    18061 agtatcatct gcgtgatctc aaaagatgca ggtaattcgc tgattctttc tgcgacagga
    18121 ccaatggcga cagaaaaatt atttttttgt cccttttcta attcatggac caacgtttgg
    18181 gctacctgat aactggtctc gatcacattt tcttcaagag aatcaactat taaaaatttg
    18241 atgaatcgag aggaaacagc agagaagatc acttgttgat tctccctaaa taaaaacttc
    18301 aaataatcac tgagatgagc gtaatcttca aagtctgtac tgacttggtt tgctgctaat
    18361 aatacggtca tttttttccc tgctaaagag cgattgaaag attcagcaag tttcaatgtt
    18421 tcactgatcg taagtgagcc tttgaacaaa ccgttcaaga aatgattttt tttcaattcg
    18481 aaaacaagat cagaagaagt gtgcgtttct ttttgctgca aatctaaagc agttactgct
    18541 ttttctattg cttgtttcaa ttcgctactt ttaatcggtt taagcagata atcatctgcc
    18601 tgtacctgga ttgctccttt catataatcg aaatcgtcga atccactaat aaatatgaga
    18661 cgagtccaag gaagtagttt ccttgcttct tttgaaaaag tcaacccgtc catgaaaggc
    18721 atttggatat cagtcagaat gatgtcgggc tgtaagtcga taattgaaga cagtgccatc
    18781 tcaccatctc cagcttcgcc gacataagtg attggataat aagaaccaag gacagttatt
    18841 tgttcccgca agttattgcg aatgaggtat tcgtcttcga tgataaaaac tttatacatg
    18901 tttgtttcct ccaattgttt tgggagatag agggatcatc agtgtgacag ttgttccttc
    18961 tcggtatttg ctgtcaatcg tgataggttt ttgatcattt ccgtaataaa gaaggagacg
    19021 cttgtttacg ttgtaaagtc cgtagccgat actgaaatct gaatcgatcc ctttggctaa
    19081 ctctttacgt atttctgcca gtttttctgc agggatgcca atcccgttgt ctataacttt
    19141 gaattggatc atctcatcaa ttttgcatgt ggtaatacga ataaatcctt tgtgacggac
    19201 gtacttcgtt ccatggtata cagcattttc aatcaatggc tgcaagatca tttttaaaat
    19261 agaaacagga aaaagtgttt ctggtatatc gatcttatag tcaagcatat ggccgtatct
    19321 tatttttaag atgactagat aatcattgat atggctaatt tctttttcaa gaggaatcca
    19381 gtcttgtcct ttgcttaaag acgtacgaaa aaagccggaa agtgcatggg tcatagtcag
    19441 agcttcttca aagcagtctt gttcgatcaa agaaataatg gcatccaaac tgttataaat
    19501 aaaatgggga gtaatctgag cttgcaatac acgaacttca ctttgtgcta aatggtattg
    19561 tttcaaagcg ttctcccgta aagactggtt tagatcttcc gccattttat tcatgctagc
    19621 ggtaagtgtt tgtaattcca gtgtttccgg cagggttaaa cgaaagccaa ggttccctga
    19681 agaaagttgt tctgacatct cgatcagctg attgatcggt tcttggacct ttctctctaa
    19741 aaagcgacga ttctttttcg catagaaaaa aatggtacta agaactccaa gttccactat
    19801 tgttaataga atgagcgagc ggacaagtgt cgtatttttt ttgcttgcta agtcgatctc
    19861 cacacgaaca aaatcttgta agatatctga cagcagttga gtgacagaat ctacctgatt
    19921 gatgatctct tcattggtat ccataaaatc ttttgccaag atattattga tcagctcgtt
    19981 ttgataatct tccagtgtat cgatcgtacg tagcgataca tctaacgtac taatttcttt
    20041 aagtgtattt gtattatttt tgctctctcg aatatcattc ctaagttctt ctaggatatt
    20101 ttcctcattg aactcatcta cggttacttg gccagaaatc aagtcccaca tactttccaa
    20161 aactttttga tcaaccttca aagcaattgc atctgctttt tggatatttg cgatcgtctg
    20221 ttgataaatc aaaagctgtt gcgaatataa cagtatgctc agtagcaaag ggataacagc
    20281 aaatagaagc atggctttgt ttgcttgggc aaaaatctgt tttatgctta aattttttgt
    20341 catgatactt ctccagtcaa taatttctgg ttgggatgtt agctatattc tgctgggaaa
    20401 agacagtctc ggaaataaaa atatctttag gtaatgtttt tcctgtttga atatttccaa
    20461 aataacgagt aacggtattg gctacatacc agccgacatc aggtttacac tcgactacac
    20521 aattgacgat tcctgagcgt agcttctcga tcatatcttc ttgtccatca atagttacaa
    20581 tgatcaaatc ttctccaggt ttgattgatg tttcttttat cgcagctaaa gcaccgtgag
    20641 tcatttcatc actatggctg tataatacat cgatttcgga taaatcattt gtttctaaat
    20701 atcgtttcat ctggtctttt cctttcatcc gaataaaatc accgacaatt cggtgaacaa
    20761 tagtcatcgg aggtttatgg tcgatggttc catctttttt tagggttggt ttacgattga
    20821 ttgattccaa aaatccttcg gagcgtagct tggtcggtgt ggagttctct gaaccagcaa
    20881 gttctaggat tctgatttcc tgtttctttg aatttgtgaa gtgattgctg acaaataaac
    20941 ccgcccgatt tccttctgct ttgaaactgg ggccaatgtg ggtgacgaaa agctcgggat
    21001 cattcgtttg aatatgtctg tcgacaagta tgacagggat ccctgctcgc tttgcttctt
    21061 ttaatacaga gtcccacccg gattcttgca aaggagtaaa aatgatcata tccactttat
    21121 aggcaatgaa cgtccgtata tcttggattt gccgttcctg attcatgtag ccatttcggt
    21181 agagaacttg gtaatcttct ttttcaagcg cagtgataat tgattcagta tgctttttcc
    21241 gccagctact ctccgttcct gattgcgaaa aaccaacgac tagtgggtca ggtgcttcct
    21301 ttgtattagg tgtacacccc atcatttggt aagcgattac aacaagggct aaaaaaaata
    21361 aaacacgttt cactttaaat cactccatct ggatttgtta gtactatctt atcagataaa
    21421 agggaaaatc aaagtcaaaa ggaatcaatc gaaaaaaatc caagaaactt aaaaaaatcc
    21481 aagacaaaaa aagtacacct aaaaaattta aagtaaatcc cataaaaaaa tcaatgttaa
    21541 cgctttcttt attagctata ctcggtgtat caattgaaat gggggataaa aaatgaaaaa
    21601 caaatttaga aagattgttg ggactatcgc tattttgacc gcaagcattc tattaagtgc
    21661 gtgtggcaat tcaggatctg cagatgatag tacggggtat gtaggaatcg ctatgccgac
    21721 caaatcagca gaaagatgga tcgctgatgg gaacaacatg gtctcggaat tagaaaaact
    21781 gggttataaa acagatcttc aatatggaga agataaagta gaaaatcaag tagcacaaat
    21841 agaaaatatg attacaaaag gggtagatac acttgtgatt gcttctatcg atggttcggc
    21901 tttaactgat gtgttagcta aagcaaaaga agcagatatc aaggtgatcg cctatgatcg
    21961 tttactgatg aattcagaaa atgtcgatta ttacgcgact tttgataatt ttggagtagg
    22021 tgtatcgcaa gcagcttata ttgaagagca cttaggattg aaagaaggaa aaggaccatt
    22081 cacgattgaa ttatttggtg gctcgcctga cgacaacaac gcactaatca attacaacgg
    22141 agtgatgtct gttttacaac catatatgga caacggtcag ttggtcgtgc catctggaca
    22201 gacgagtttc agtcaaatcg ctacgcttcg ctgggatggt tctacggcgc aagcaaggat
    22261 ggataatctg ttaagcgcaa attatactga ccaaacattg gatgctgttt tgtcgccata
    22321 tgatccaata agtttaggaa tcatttcgtc attaaaaggc gtggggtatg ggagtgaaag
    22381 taaaccttta cctgtgatta ctggacaaga tgcgacagtt gctggcgtta aatcgatcat
    22441 tgccggggaa caaacacaaa cgatctttaa agacacacgt atcttagcaa aaaatacgat
    22501 tgaaatgatc aaagcaatca gtgatggaga agaggttccg gtcaatgata ctgaaactta
    22561 tgataatggg gtgaaaactg tccctacgta tttagcaaat acagtatctg tcgataaaga
    22621 taattatcaa gcagagctga tcgacaccga ttattataaa gaatccgatc tgaaaaatta
    22681 agaggaacca aacaaggaac taaagagatc taagtcagag agaggataag aattatggcg
    22741 gattgtattt taaaaatggc agatatcacc aaaaaatttt ctggaatccg agcgttagat
    22801 tgcgtgaatc tgatgattga acgtggggaa atccatgcat tgtgcggaga aaacggagcg
    22861 ggaaaatcaa cattgatgaa tgtcttgagc ggcttgtatc catatggctc ctatgacggg
    22921 gaaatctttt atgatggaaa gctgtgtcaa tttaaagatt tgaaagatag tgaagaaaaa
    22981 ggcatcgtca tcattcatca agaattagca ctaagtcctt atctttcaat aaaagagaat
    23041 atttttctcg gaaatgaaca agcagaaaga ggcgtgattg attgggaact gacagaaaag
    23101 aaaacatatg aactgctgaa taaagtcggt ttgtctatcg acccgaatct tctagtatcc
    23161 cagattggcg tagggcaaca gcaactagtt gaaatagcta aagcattatc aaaatccgtc
    23221 cgtctgttga ttttggatga gcctaccgct gcattgaatg aagaagaaag cgcgaatttg
    23281 ctgagattga tccgagagtt gaaagagcaa ggtgtaacct ccatcattat ttcacataag
    23341 ctgaatgaaa tcgtggatat cgcagatcga atcaccattt tacgggatgg acagacgatt
    23401 gagacactag aaaatgaaat gatcagcgaa gagcatatta ttcgcggaat ggtgggcaga
    23461 gatttagttg atcgttatcc tgaaagaaaa ccggatattg gagaagttta ttttaaagta
    23521 gaagactgga cggtctatca ccctcttgat cacgagcgaa tcataaacga ccacatcaac
    23581 ttgcatttga aaaaaggaga gattgtagga atagctggac tgatgggagc gggcagaact
    23641 gaattcgcta tgagtctatt tggacattct tatggaagca agatatccgg acgtatatta
    23701 aaggaagggg cagagatatc agttaagacc gttccttcag ccattgcaaa cggactcgct
    23761 tatgtctcgg aggaccgaaa atctttagga ctgaatctgc tgatggatat tcgggaaaac
    23821 gtgacgctga gcagtttgga taaaataagt caaaaaggag tattagataa agaaaaagaa
    23881 gtaactgagg cagaaaattt ccggaaaaag atgcgtataa aaacaaactc catccatcaa
    23941 attgtcagta gtcttagtgg tgggaatcaa caaaaagtcg ttttatccaa gtggctgatg
    24001 acacaacccg atattctttt tttggatgag ccaacccgtg ggatagatgt tggggcaaaa
    24061 tatgagatct acacgattat cgaagaaatg gcagcaaatg gcaaagctgt ctgtcttatc
    24121 tcttctgaac ttcctgaaat attagggatg tgtgatcgaa tctatacgat gaacgaagga
    24181 cgtattacag gagaagtctc gcgagaagat gcagatcagg aagtcttgat gaaattaatg
    24241 acgaaagaag aggtgggtta agttggaaca ggcaaaaaat cttacggata aaaaagagaa
    24301 aaaactaaat ttgaaaagta gagcagctga tctttttggt cgctacagca tggtgatcat
    24361 tttagcggga ctacttattg cgtttcaatt gatgacggat ggtattttct ggcgtccatt
    24421 gaatataact aaccttgttc tgcaaaatag ccatatatta gtactagctg cagggatgtt
    24481 attagttgtg ctcttaggtc atgtggattt gtcggtaggg tcagtgatgg cgtttgttgg
    24541 tgcaattgcg ggcatgatga tggtcaatcg ccaaatgaac ccgtggatga cagttatcgc
    24601 atgtcttgca gtaggcgggc tgatcggcgc atggcaaggt ttttgggtag cttatgcagg
    24661 gataccggcg tttatcgtga ctttagcagg attgctgatg tttcgtggac tgacacaagt
    24721 tgttctagga ggccaatctc tagcaccttt tccagctgtt ttccaaaaaa tgtctacggg
    24781 ttatttacca gacttaagcg aaggaagcag ccttcacttc ttgacattga ttttaggaat
    24841 gatcatcacg atcgtgttag ttgttggtca atggcgaaca agaaatcgtc ggaaaaatca
    24901 tttatttgaa gtggaatctc caacagcttt cttggtaaag gcggtattta cagcagtcgt
    24961 tgtactagga ataacttata tctttgcctc ttatcaaggg tatccgatta ttttgatcat
    25021 tcttggtttg atcgttgcag catatgggtt tttgacaaat aaaacagtag caggtcgaca
    25081 gatttatgcg acgggcggaa atcgaaaagc ggctgaacta tcagggatca aaacaaagcg
    25141 aatcacgttc tgggtattcg tcaacatggg agtcatggca gctttagctg gattagtttt
    25201 agctgctcga ttgaatgcgg caacacctca agcaggtaca tcgatggaac tggatgcaat
    25261 ggcggcagta tattttggtg gtgcttcgac ttccggcggc attggaacaa tcatgggtgc
    25321 gattgtcggt ggcttggtca tgggtgtatt gaacaatggc atgtcgattt taggcgtcgg
    25381 ggtagactgg cagcaggcaa tcaaaggatt gatcttgctt ttagccgttg tgctagactt
    25441 gtataataag aaaaaacgaa caacgtagga gaacgccggt tattcagaaa aaagactgtg
    25501 acaagacatt tgtcacagtc tttttttcgc taaaagcgaa cattcattgt ttcgtttgta
    25561 tagactaaaa ataatatatg tttttttacc attcgtataa attctctttt tgctcttgat
    25621 ttttttgctt aattgagtta aattacatag ggcgctaaaa aaagccttga ttaaattaat
    25681 aatgttcgtg ttttgctgga tgtgaacatc aggaggataa tgtaatgaaa gtatttgatt
    25741 acgaagatat tcaattaatt cctaataaat gtatcgtaaa tagccgttct gaatgtgata
    25801 caacggtcac attaggtaaa catacattca aaatgccagt agtgcctgcc aacatgcaga
    25861 cgattattga tgaaacaatc gcagaattct tagcggaaaa tggttatttc tatatcatgc
    25921 atcggtttga tgaagcagct cgaattccgt ttatcaaaaa aatgaaaaag cgtggattga
    25981 ttacctcgat cagtgttggc gtcaagaaag aggaatattc ctttatagag aagttggcag
    26041 aagaatcgct aaatcctgat tatatcacta tcgacatcgc gcatggacat gctaactcag
    26101 ttattgatat gattcagcat attaaaaaat atttgcctga aacttttgtg attgccggta
    26161 atgttggtac accggaagcc gtccgtgaat tggaaaatgc gggcgcagat gcaacaaaag
    26221 tcgggattgg tccaggaaaa gtatgtatta caaaaatcaa gacaggcttt gggactggag
    26281 gctggcaact ggcagctctt agatggtgtg cgaaagcagc tcgcaagccg atcatcgctg
    26341 acggtgggat cagaacacat ggcgatattg caaaatctgt ccgttttggt gcgacaatgg
    26401 tcatgattgg ttcattgttt gccggtcacg aggaatctcc tggcgaaaca aaagtcgaaa
    26461 atggaatcgt gtataaagaa tactttggtt ctgcttcaga attccaaaaa ggcgaaaaac
    26521 gaaatgttga aggaaagaaa atctggatcc aacacaaagg atctttgaaa gatacactag
    26581 tggaaatgca acaagacctt caatcatcta tttcttatgc tggaggaaga gatctggaag
    26641 ccattcgaaa agttgactat gtcatcgtga aaaattcgat ttttaatgga gacacaatct
    26701 aaaaaataaa taaaatatta ggatgttgtg aaaaagttgt cctgcattaa gtaataagaa
    26761 cagccaaaca aaaatagttc tttatatgtt ttgtttggct ggaattatta tcgcagatac
    26821 aagcttttga acatcattat tcagaataag ggactgtgat aagatttttg tcacagtccc
    26881 ttatgttttg caaaaaaagt ttttcttttt tctcgtaaat tcggtaaaat caaaagaaaa
    26941 aatagacaat agaaaagaga agcttatgtg ggaaaaagta gagaaaaaac aaggaccgat
    27001 ttatcgtcaa attatggaac agattatgag agaaatcgaa aacggaagga tcaatcctgg
    27061 aagtcaactt gaatcagaac gaaaactagg gcaacttttt ggtgtgaatc gttccaccgt
    27121 tgtccatgca ttggaagagc ttagggagct tggggtacta acgagcaaac gtggcagcgg
    27181 atggtttgtt aatcagactg aatgggggaa attcgctgtt ccaagaatcg attggcggca
    27241 aatgatctct cctcgttatg agcagacgga ctggtatgag caaaaaataa gagaagcaaa
    27301 gcagatccaa aaaacatcgt ttcttgattt atacgctagc gagatgccag atgagctttt
    27361 gccgaatttt gagtttcctt cttattctct tgaagaaatc ttagcggaag aaaaagaaat
    27421 ggatctttta ggctatactc cgttacgtaa caaaatcaaa cggtatttgg aaagtaaatt
    27481 tgaattggca ttttccccca accagcttct tgtgactggt ggcggtcagc aagcaatctt
    27541 tttgatttta caaacgattc tttcagctgg agaggggata gctgtagagt caccttcttt
    27601 tttttaccga ttacctcttt tcaaagcttc aggaacgcgt ttatttggga taccgatgga
    27661 tgaagaaggg atcgacttag aaaaactgaa agaatcgatc cataaaaata aattgaaagc
    27721 agtgcttatc aacccgaatt ttcaaaaccc tacaggaaaa gtgatgagtc aaaaacgacg
    27781 agaaaagcta gtagaattgt gtcgacagta tcggcttcca attattgaag acgatgtatt
    27841 tacggatctt tcatttctaa accaagaaga agtccttcct atacgaacga ttgatccgga
    27901 taatgtgtta tacgtcggtt cgttatctag ggttttagga aaaacaacga aaatcggttg
    27961 ggtgatagga cctgagactt ttatctcgca actagctcaa gcgcaagaaa tgatggagtt
    28021 ttcgatgaat atccttacac aaatcacagt ggctaatgta tttaatgaag caattgattt
    28081 gaaaatgcaa gatgtaagaa aaaagttgaa agaaaaaagc cgactgataa gcgcttgggg
    28141 agattcacaa tcgtttttca agcttcattc aattttaggt ggttactatg cctggattac
    28201 ttgggatggg aaaaaactag accccgaact agcagaaaaa gtagtagata tgggtcttgg
    28261 aattgcacca agtctacttt ttgggcaaga tataaatgga atcagaatca attacagtag
    28321 gctggatgaa aaccaaatac cgttgtttta ttcaaagatg aaaattctag aaaagtggtt
    28381 aacagaataa tttcacaaag taaatagatt aaagaatata acagcttgga tttaaatttc
    28441 aagctgtttc ttttttttcg gaaaaagaat acgatataaa gaaactaaga aaaaaccatt
    28501 atttgtacgt ccgttcacga tgtgtaaata aaataaatgt ttgcaacaaa actgtataac
    28561 accaacgttt ttaaatagaa acagatggtt gtaagtttga ttagaaaata gaaaaaatgc
    28621 agatgaaata taaaacgctt tccttattgt gattttattc actaatgctg ttaatatgcg
    28681 gattggcata atttcctttc atcgtgaaaa tttgtgcttt tagcttgtat ttctcataaa
    28741 tccatgataa tttattaatg aagacggtta aatagtgata cagttttatt cactgtttca
    28801 atcgcagaat gaagagattt tcttatagat gagagaggaa tgtgtgacaa atggcaaaca
    28861 aaaaagcacc gatcgacttc caagcactgc tagatgaagt caatgcagat ttcccagtat
    28921 atcaagcact tgatcaagat ggaaacgtag ttaacaaaga tttagtacct gatttatccg
    28981 atgatgaact cgttgaatta atgactcata tggtatggtc tcgtgtatta gaccaacgtt
    29041 caacagcgct gaaccgccaa ggacgcctag gcttttttgc accaactgct ggacaagagg
    29101 caagtcagct ggctagtgct tacgcatttg ataaagaaga tgttcttctt ccaggttatc
    29161 gggatgtacc tcaactagta aaacatggtt tacctttatc tcaagctttt ttatggtctc
    29221 gaggacatgc tgcaggtaat tcttatccgg aagaattgaa agctctacca ccacaaatta
    29281 tcatcggtgc acaatatgta caagcagccg gtgttgcatt aggtttgaag aaacgaaaca
    29341 agaaaaatgt cgtcttcact tatactggtg atggcggttc atcacaaggt gatttttacg
    29401 aagcaatcaa tttcgctggc gcttaccatg caaatgccgt attttatatc caaaacaacg
    29461 ggtatgcgat ctctacacct cgtgaaaaac aaactgcagc aaaaacactt gcacaaaaag
    29521 ctgtcgcagc tgggattcct ggtattcaag tcgatggaat ggatccgctt gcagtatata
    29581 ctgtatctaa aatggcacgg gaatgggcga tcagtggaaa tggaccagtc ctaatcgaaa
    29641 cactgactta tcgttatgga ccacatacac tatctggtga cgatccaacc cgctaccgta
    29701 caaaagacac agatgacgat tggcagaaaa aagatccatt gatccgcttc cgtaaatatt
    29761 tgacggaaaa aggactttgg tctgaagaaa aagaagaaca agtcatcgaa gcaactaaag
    29821 aagaaatcaa agcagctatt gctgaagccg acaaagtacc aaaacaaaag gtttcagatt
    29881 tcttgaaaaa catgttcgaa gtatctccac aatcaatcaa agaacaaatt gcaatttatg
    29941 aagcgaagga gtcgaaataa tcatggcaca aaaaacaatg atccaagcaa tcacagatgc
    30001 cttagcactt gaactagaaa atgacgagaa tgtcgttgtt tttggtgaag atgttgggaa
    30061 aaacggagga gttttccggg caactgaagg attgcaggaa aagttcggag aagaccgagt
    30121 attcgatact cctttagctg aatctggaat cgcaggattg agtttcggtc ttgcgttaga
    30181 aggatttcgt ccagttcctg aaatccaatt ctttggtttt attttcgaag caatggacga
    30241 agtagtagct caaatggctc gtacaagata tcgaatgagc ggcactagaa atctacccat
    30301 cacaattcgt tcgccatttg gtggcggtgt tcatacacca gaattgcatt cggataattt
    30361 agaaggtttg atcgctcagt ctcctggtat tcgagtagtg attccttcca acccttatga
    30421 tgcaaaagga ctattgattt cttctattag aagtaacgat ccggttgttt tcctagaaca
    30481 catgaaacta tatcgctcat tccgagaaga agtgccggat gaagcttacg aagttccttt
    30541 ggataaagca gcagttactc gtgagggaac agatgtatct attatcacat acggagcaat
    30601 ggtgcgtgaa gcaatcaaag cagcagacaa tcttgcaaaa gaaaatatat ctgttgaaat
    30661 cattgatctt cgtacagttg cgccattaga tgtagaaacg atcattcaat ccgtggaaaa
    30721 aactggtcgc gtagttgttg tccaagaagc acaacgacaa gcgggcgttg ccgcgcaagt
    30781 agtttctgag atctcagaac gcgctatcct ttcattagaa gccccaatcg gacgagtttc
    30841 tgcacctgat acagtcttcc catttggaca agcagaaaat gtctggttgc caaatgcaaa
    30901 agatatcgaa gacaaagtaa aagaaatcgc ggaattttaa gaaaagaagg gacgaaacaa
    30961 agtcatggct tatcaattta aattgccgga tatcggtgaa ggaatcgcag aaggtgaaat
    31021 cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa gatgatacat tactagaagt
    31081 tcaaaatgac aaatcagtag aagaaatccc gtcacctgtt acaggaacgg ttaaaaatgt
    31141 gattgttcca gaaggtaccg tagcaaatgt tggggatgta ttagtagaaa tcgatgcacc
    31201 aggacacgaa gacaatgaag gggacagtgg tgtagccgca gaatcccaaa ctccagctaa
    31261 accggctgct gaacccactg ttgatacaga atcagctgga tcgtcaagcg aaggagtctt
    31321 ccaatttaag ttaccggata tcggtgaagg aatcgcagaa ggtgaaatcg tcaaatggtt
    31381 cgtcaaacca ggagatacga tcaatgaaga tgatacatta ctagaagttc aaaatgacaa
    31441 atcagtagaa gaaattccgt cacctgtcac agggacagtt aaaaacgtga ttgttccaga
    31501 aggcactgta gcaaatgttg gggatgtatt agtagaaatc gatgctcctg gacacaatag
    31561 tgcaccgtca actagtgcgc catcagctga agcaccaaaa gaaaaagtgg aaacatcagg
    31621 ttcagcaagt gtcgttgaag ctgccgatcc aaataaacgt gtattggcta tgccatctgt
    31681 tcgtcaattt gcccgtgaaa aagatgtaga tatctcacaa gtaactgcaa ctggaaaagg
    31741 tggcagagta acaaaagagg acatcgaaaa cttcttggca ggtggtccaa gcagtgcacc
    31801 agcgaaatca gaagcgccag aagcggcagc accgaaagaa gcagctcctg cagcagaaag
    31861 caaaccagct gcaccagcaa aaccattcaa gtccaactta ggagacttgg aagaacgtgt
    31921 agcaatgacg ccaacacgta aagctatcgc taaagcaatg gtcaacagta agcatacagc
    31981 tccacacgtg acattgcatg atgaagtaga agtgtctaaa ctatgggata atcggaaacg
    32041 atttaaagaa gttgctgctg caaatggtac gaaacttact ttcttgccat atgtcgtgaa
    32101 agctttaaca gcgactgtta agaaataccc agtattgaat gcatctatcg acgatgcaaa
    32161 ccaagaaatc gtttataaac attactacaa tatcggtatt gcaactgata cggatcacgg
    32221 actatatgta ccaaatgtga aagatgcaga ccgcaaagga atgtttgcta ttgcagacga
    32281 aatcaatgag aaagcaaaat tagctcatga cggcaaacta tctgcagaag atatgcgcaa
    32341 tggaacaatt acgatcagca atatcggatc tgtaggtggc ggttggttta caccagtcat
    32401 caactatcca gaagtagcta ttttaggtgt aggaacgatt gcacaacaac caatcgtaaa
    32461 tgctgaaggc gaaatcgttg tcggtcgtgt gatgaaactg tcattaagtt tcgatcaccg
    32521 tatcgtagat ggagcaactg cgcaacaagc aatgaataat atcaaacgtc ttttagctga
    32581 tccagagcta ttaatgatgg aaggatgata aacaaatggt agttggagat tttgctgtag
    32641 aattagatac agttgtaatt ggtgcgggac ctggtggcta tgtagctgcc atccgcgctg
    32701 ctgaaatggg acaaaaagta gcaatcattg aaagagagta tattggcggt gtttgtttga
    32761 atgtcggctg tattccttca aaagcactga tagctgcagg acaccattat caagagtcac
    32821 tagattctga aatgtttgga gtaactagtg aaaacgtcaa actagatttt gcaaaaacac
    32881 aagaatggaa agaaaacaaa gttgtccata cgttgacttc tggtgttggt ttcctattaa
    32941 aaaaacataa ggttgaaaca atcgaaggag aagcgttctt cgtagatgat cacacactac
    33001 gcgtcattca tccagattca gctcaaactt attcgttcaa taacgcaatc atcgcaacag
    33061 gttcccgtcc aatcgagatt ccaggattta aattcggagg acgtgtgtta gattctactg
    33121 gcggattagc attgaaagaa gtacctaaga aatttgtgat catcggtggc ggtgtgattg
    33181 gtgccgaatt gggcggagcg tacgcaaact taggagcaga agtaacgatt cttgaaggct
    33241 caccacaaat tttaccaact tacgaaaaag atctagtaaa attagtagaa gatgatttca
    33301 agaaaaaagg cgtaacagtc gtaacgaatg caatggcgaa agaagcagtc gataacggtg
    33361 acagcgtgac agtgaaatat gcagtggacg ggaaagaaga atccgttact gctgattatg
    33421 tgatggtcac tgtaggtcgt cgtccgaata cagatgatat gggcttagaa caagcgggtg
    33481 tcgaagtagg agaacgagga ttgatcacgg tggataaaca aggacgtacg aacgtaccta
    33541 acatctatgc catcggtgat atcgttccag gggcggcatt agcacacaaa gccagttatg
    33601 aagcaaaaat cgctgctgaa gcaatctctg gcaaaaaagt agctgtagat tataaagcaa
    33661 tgccggcagt agccttcact gatcctgaat tggcttctgt aggtatgacg atcaaagaag
    33721 caaaagatgc tggtatcgaa gcaaaagcat ataaattccc attttcaggg aacggtcggg
    33781 cattatcgct tggtaaaaca gaaggattca tccgtctagt tacgacgatc gaagataacg
    33841 tgcttattgg cgcgcaaatc ggtggagtcg gcgcaagtga tatggtctct gaattagctt
    33901 tagcaatcga atcaggaatg aatgctgaag atatcgcctt gacgattcat cctcatccat
    33961 ctcttggcga aatcgtcatg gatgcttcgg aattagcatt aggtttacca atccatattt
    34021 gatttgaata atacaggcga aacgcaactg aaaaatcagt tgcgttttgt tttttagaat
    34081 tatcaataat attattatgt aaacttaaaa atatgacaac ttcttggttt attaccactt
    34141 cctatgctac actgaagagg aatatgaaat aggagagttg aagatgcaag aatatcagta
    34201 tccacttgat ttagattgga caacagacga aatggtcatt gtaatgaaga tgtgggaaga
    34261 tctggaacgt gcgaatgaaa aagggataaa caatgaagct tttttgaaga cttatcagca
    34321 gtttaaaact gttgtcaaat ccattggtga ggaaaaacga ttgggacggg aatttgaaaa
    34381 agcttccggt tattctttat atcgtacagt gcagcaagca aaaaaaaatc caaacaaaac
    34441 tttgagaatg aacgggtgag ataaatggaa cagatgattg atcatatcaa gtcctggctt
    34501 gaagaagctg gtgagaaaat caaatcttct caacaaacat taacaatctc gcaaaaatca
    34561 aatcgaacgg atctagtaac aaatgtcgat cgaaacatcc agaattttct aatcgaaaaa
    34621 atccattcct ttgatccaga ggcgaaaata ttggcagaag aagaagggtg taatcaatta
    34681 tcggatctcg atggaagagt ctttatcatc gatcctattg atggcacctt gaattttgtg
    34741 attgaagggg aaaatttttg tattatgctc gctgtttatg aatccggcgt tggaaagctt
    34801 ggtttcattt atgacgtgat gagaaatgaa cttttttggg gtggaaaagg aataggcgtg
    34861 tataaaaatg atgagccatt gcctagacca gcaaataaat ccttatcgga aggcttactt
    34921 ggtgtgaata gttatctttt cggtcataat aaatatggaa tccgagaaat tgcggaaagc
    34981 agcatgggga cacgtatgta cggatgcgct ggtcttgaac tagtggcaat cttgaaagga
    35041 aaccacattg cttatatttc aaaccttagc ccttgggatt atgcagcggg aaatgtctta
    35101 ttggaagagt ttggtatggt aacaagcggt ctttttggta ctcctttgac atttgaaaaa
    35161 agggaatact atttagcagg gacttcttgt gcatatgcag aaatcgcaga ccttttgaaa
    35221 aaagaaggct gaaaaaaagg tagaaatcat tcacaaaaat gataaaaacg agcaaaagat
    35281 taattttctc gaaaaaatga aaacatttta ctatatttca tgaaaaaagt ctgctataat
    35341 tagcagtcga gtaaaatttg ccctgtcata aagaaccaat aaaaaaggag ttagttacat
    35401 tgaattatag aaacgatatc cgtaacgtcg caattattgc ccacgttgac cacggaaaaa
    35461 caacattagt tgatgaacta ttaaaacaat cagatacatt agacgcacat acgcaattgc
    35521 aagaacgtgc gatggactca aatgcgctag aaaaagaacg tggtatcacg attttagcca
    35581 aaaacacagc tgtagactat aaaggtattc gcgtaaacat catggatact ccaggacacg
    35641 cggacttcgg tggcgaagta gaacggatca tgaaaatggt tgacggcgtt gtgttggtcg
    35701 tagatgcgta tgaaggaaca atgcctcaaa cacgttttgt attgaaaaaa gcactggaac
    35761 aacatatcac accaatcgtt gtcgtaaaca agattgacaa accttctgct cgtcctgagc
    35821 atgtcgtaga tgaagttctt gaactattca tcgaattagg agcagatgat gatcagttag
    35881 actttcctgt gatttatgca tctgcactaa atggtacgtc aagcttgtca gatgatccag
    35941 cagatcaaga accaaccatg gcacctattt ttgatacaat tattgaaaaa atccctgctc
    36001 ctgtcgataa cagcgacgaa ccattacaat tccaagtttc attacttgac tacaacgatt
    36061 atgtaggtcg tatcggaatc ggacgtgtat ttagaggaac aatcaaagta ggagaccaag
    36121 tagcattaat taaattagac ggaacagtta agaaattccg tgtaacgaaa ttatttggtt
    36181 tctttggact aaaacgtcta gagattcaag aagcaaaagc tggagacttg attgctgtct
    36241 caggtatgga agatatcttt gtcggtgaaa cagttactcc tgtggatcat caagatgcgt
    36301 tacctatcct tcacattgac gaaccaacat tgcaaatgac tttcttagtt aataattcac
    36361 catttgccgg tcgagaaggt aaattcgtaa ccgctcgtaa gattgaagaa cgtttgatgg
    36421 cagaacttca aacagatgta tccttacgag ttgaaccaac aaactctcca gatgcatgga
    36481 cagtttctgg tcgtggagaa ttgcatttgt ctatcttgat tgaaaacatg cgccgtgagg
    36541 gatacgaatt gcaagtatct cgtccagaag ttattgaaaa agaaatcgac ggtgtaaaat
    36601 gcgaaccgtt tgaacgcgtg caaatcgata caccagaaga atatatgggt agtgttatcg
    36661 agtccttgag cttacgtaaa ggtgaaatgc aagatatggt ccatacagga aacggacaaa
    36721 tccgtttgac attcttaaca cctgcccgcg gtttgattgg ttattcaact gaattccttt
    36781 caatgacacg tggttacgga atcatgaacc atacttttga ccaatatctg ccaatgcttc
    36841 ctggtcaaat tggcggacgt caccaagggg cgcttgtttc gatcgataca ggaaaagcaa
    36901 cgacttattc tatcatgagt atcgaagaac gcggtacagt cttcgtagaa ccaggtacag
    36961 aagtatatga aggaatgatt atcggggaaa acagccgtga taatgatttg actgtcaaca
    37021 tcacgaaagc aaaacaaatg acaaacgtac ggtctgctac taaagaccaa acatctgtca
    37081 tcaaaaaacc aaaacaattg acgttggaag aatctttgga attcttgaat gatgatgaat
    37141 attgcgaagt aacaccagaa agcattcgct tgagaaaaca aattttagag aaaaatgctc
    37201 gtgaaaaagc atctaaaaag aaaaaataat ctgtaaaagg atgaagagag aaaccagctt
    37261 gttataaaaa atagctggtt tctttttttg tccaactctt ttctgtggag aagtcatctt
    37321 tgtcaatgga cataaattca gaaaatacgc cgaaagacgt ttaaaagact tttaaaaaac
    37381 ttatttctag agagtttttg tttcataata agataatgtt tcacgaaagg agatcaactt
    37441 gtccttcgaa gaaaatcgaa gtaaaatggc aagagaaagg agcgggaaga tgaacgactt
    37501 attaaaacaa tactttggct atgatgagtt tagacctggt caaaaagaaa ttatccagaa
    37561 agtcattgat caggaaaatg tattaggaat catgcctacc ggaagtggga aatcaatctg
    37621 ttaccagctt ccagctttac tattagatgg gcttactgtg gttgtgtccc ctttaatctc
    37681 tttgatgaag gatcaggtgg acgcggcgaa tcaattaggt atacccgcca cttttatcaa
    37741 tagctcgtta gatggttacg aaacagctag acgcttccaa gaaatcgata gacagcagta
    37801 ccgtttactt tatatcgctc ctgaacgttt tatcatgccg gattttatcc aagcaatgaa
    37861 gcgatggaac gtccgtatga tcgctattga cgaggcacat tgtatctctc agtgggggca
    37921 tgatttccgc ccaagttatt tacagatggc gaaccagcta gatcaactgc caaatcgtcc
    37981 tgtaattgtt gctttgacag ctacagcgac tgtgcaagta gctgctgaca taaaaagact
    38041 tttaaaaatt ccagaaaaca accatattca gactggtttt gaacgagaaa atctacgatt
    38101 tcaagtaatt aaagatcaaa aaaaagaaca atatctaatc gaatatttaa agatcaacaa
    38161 aaatcaatct ggtatcattt atgcagcaac gagaaaagaa gtagaccgtc tctatcattt
    38221 gcttagaaaa ttcgattttt ccgtgggtag atatcatggt ggtctaaatg agaatgagcg
    38281 aacagaaatg caagaagcct ttttatacga tcgcttgcag ctgattgtag cgacaaatgc
    38341 atttggtatg ggtataaata aaagcaatgt ccgttttgtt atccattatc aaattcctgg
    38401 ctctctagaa gcatactatc aggaagctgg tcgggcggga agagatggtc tttccagtga
    38461 agccatcctg cttttctctc ctcaagatat ccaagtccaa aagtttttta tccagcagtc
    38521 ccaaagagaa gaagggcaaa agcaaaaaga atatgaaaaa ttaaaagcga tgacagaata
    38581 cgtgtatatt gagtcctgtc ttcagcaata catcctaaac tattttggcg agacaagcag
    38641 tccgtgcaac cgttgtggta actgtttaga tgacagagaa atagtagaag taacgacaga
    38701 agcacagatg gttctttctt gcttaaaacg tatgggtgag aactatggta aacaaatgct
    38761 gatgaaagtc ttagcaggat cgaaagaaca aaagctgcaa gctttaggtt ttggacattt
    38821 atctacctat ggactgatga aaaaccaatc gcaaaaagaa acgatgcagt taattgaata
    38881 tttgatatcg aatggctatc ttctgaccat caatggtgag tatcctgttt tgaaagtaac
    38941 agaaagaggg atacaagtat tgaaaggaca agaatctgtt tatcgaaagg aacccaagaa
    39001 agtccagcag ctatcggatg aagaaaccga tacactattt gaagtgctgc gggaactgcg
    39061 aacggatcta gcatcagaag caggcgttcc cccgtatgtc gtattttcag attccacatt
    39121 gaaagaaatg agccgaatac gtccatcaag cagattggag atgcttcaga taaaaggagt
    39181 tggccaaagc aagcttgata aatatggcga agccttttta tctcggataa aaaatgcgga
    39241 ctctaacgtt ctggataagt agaaagaacg aatacgtaaa aatgaataaa aacaactggt
    39301 taaatctgtt ttttttgctc cttatgaaaa tcaaatatgt tataattaag gaattagaat
    39361 gggaggtaag cacagatgga ttctttgtcg aaaaaacttg cgatccaagt attaaatgat
    39421 gaagcagaac ggatcaaacg attaatccga aatcaaaaca acagtctctg tatctcacaa
    39481 tgcaaagcct tcgaagaagt ggtagataca cagatgtacg gcttttcccg gcaagtcggt
    39541 tttgctgtcc gtataggagt aatcggaaag aacgagggcc agctgatgtt aagtgagcta
    39601 gaacgagaat tgaatcagtt atatagtgag gtttaccaag aaatttatga taaaaaagaa
    39661 gttggcaagg aggaatagac ttgcctaaga aagtgaaaaa aaggcatcta ttagactata
    39721 gcatcttgat tccctattta attttatgtg tattgggatt gatcatggta tatagttcta
    39781 cgtcgtatct attattagaa aacggacaaa atccgtccgc atcagtcatt aaccagagta
    39841 ttttttgggt gcttagcttg attgctatcg cactacttta taaaatgaaa acagatgttt
    39901 tgaaaaacca gcgtttgatc atggcagcca tcgcagtttt gacgattttg ctgctgattg
    39961 ttgtgttttt tggtaaggag atcaatgggg cgaaaggttg gctgcaaatt gcaggattct
    40021 cgatccaacc tgccgaatac ctaaaaatca tttctatctg gtacctgtct ctgactttat
    40081 ctaaaagaca aaacagtgta caaaaagatt ttcttgggac tgtgaaacgt ccacttgcaa
    40141 tggttatcgg acttactgca ttagtcgcta ttcttcccga ttttggtaat gcggcagtta
    40201 tctttttgat tattttggtt ctgcttttag ccagcggagt caattatgtg tatactttga
    40261 ttgtaggagt aggaggcttt tgtctaagta cttttacgat ctggctgatc aatatcacaa
    40321 acggaaagat cttacccgga cgtttacagt atatctataa tcgatttgct atttaccaaa
    40381 atcctttctc agacgaatta aataaagggc atcaactagt caacggctac tatgccatgt
    40441 tcaacggagg actatttggc aggggattag gtaacagtat ccagaaaaaa ggatttttac
    40501 aagaagcaca aaccgatttt atttatgcaa tcgtagtgga agaactggga gtgattatgg
    40561 ggattttgat attagcgttg ctattcttta tgattgtgcg cattgtcctt gtaggtatcc
    40621 gttcaaaaga tccgttcaat tcgcttttgt gcattgggat cggcgcaatg tttttgatcc
    40681 aagtatttgt caatcttgga gggattactg gcgttattcc tttgacaggg atcacgttcc
    40741 catttttaag tcaaggaggc tccagtttgc tgatgctttc gatttgtgtc ggctttgtcc
    40801 tcaacatcag tgcggatgaa aaacgcaaga gcttaggttt ataataaaaa aagatgtagg
    40861 agtggttcga tgaaaaaagt tttagttgct aacaggggtg aaatcgctgt acgtgtattt
    40921 cgtgcttgta cagaattagg aattaaaact gttggaatct atgcagaaga agatgaatat
    40981 tccgtacacc gtttcaaagc ggatgaagca tatttagtag gacaaggaaa aaaaccgatt
    41041 gatgcgtatc tagatattga agggatcatt tctatcgcga aagaatgcgg agctgacgct
    41101 attcatccgg gttacggtct tttgtcggaa aacttgaatt ttgcgcaacg gtgcgaagaa
    41161 gaaggcatca tttttgttgg acccaaactg catcatttag atatttttgg agataaaatc
    41221 aaagcaaaag ctgcagctat cgaagcgggg attgcttcga ttccaggcac agatggacca
    41281 atcgcttcta tagacgatgc cttggagttt gcgaaacaat acggttatcc gattatgatc
    41341 aaggcagctc taggcggcgg cggacgcggc atgcgtgtag ctcacgatga aaagagtgca
    41401 agagagggct acgaacgtgc gaaaagcgaa gcaaaagcag cctttggtag tgatgaagtg
    41461 tatgttgaaa aatacatcgc taatcctaag catatcgaag tacagattct cggggatacg
    41521 catggaaacg tcatccatct ttttgaaaga gattgttctg ttcagcgccg tcaccaaaaa
    41581 gtagtagaag tagcgccttg tgtctctatg aatgaacagc aacgtcaaaa aatctgccag
    41641 gcagctgtcc aattgatgaa atatgtcggg tatgtcaacg caggtacagt agaatttctt
    41701 gttgaaggag atgattttta ctttatcgaa gtcaaccctc gcgtccaagt ggaacatacg
    41761 atcacagaaa tgattacaga tattgatatc gtaacaacgc aactgttgat tgctcaaggt
    41821 ttggatctgc ataaagaaat cggattgcct cagcaagaag gaattaaatt aaatggttca
    41881 gccattcaat gccgaatcac tacagaagac ccgttgaata actttttacc agacacaggg
    41941 aaaatcgata cgtatcgttc acctggtggt tttggcgtac gtctggatgt cggaaatgct
    42001 tacgcgggct atgtagtgac accgtatttt gattcattgc tagtaaaagt atgtacgcat
    42061 ggtgctacct ttgaaacagc cattcagaaa atggaacgtt gcttgaaaga atttagaatc
    42121 cgtggagtga aaacaaacat tccatttatg ctcaatgtca tcacacatcc tgaattccaa
    42181 tcaggaaatg caaaaacaac cttcattgat agtacagcaa cattatttga atttcctcga
    42241 ctacgagacc gcggaaacaa aacgatgaaa tatatcggtg aaattactgt gaatggtttt
    42301 ccaggtatcg aaagtggcga aaaaccattt tatgaagaac cacgtatgcc taaagatcta
    42361 atcacaagaa ctgattacgt aacggctaag aatgtattag atgcgaaggg tgccgatgct
    42421 ttggtcgaat ggatcaaagg acaggaaaat ttgttgttga cggatacgac atttagagac
    42481 gcccatcaaa gtctgctagc aactcgtgtt cggacaaagg atttcaagca gattgcacgt
    42541 ttgactggag aaggtcttcc tgaactcttt tcaagtgaga tgtggggggg agcaacattt
    42601 gatgtggctt accgcttttt gaatgaagat ccttggcaac ggttgagaaa aattcgttct
    42661 ttaatgccta atacgttgct gcaaatgctt ttcagaggtt caaatgccgt tgggtattct
    42721 aattatcctg acaatgtatt ggtggaattt gtcaaagaag cagcagcgca aggaatcgat
    42781 gtatttcgaa tctttgatag tttgaactgg actccacaaa tggaaaaaag tatccaagct
    42841 gtacgggata ctggtaaaat cgcagaggca gctatttgct atactggcga tatcaacgat
    42901 ccaagtcggg caaaatacaa tgttcagtat tataaagaca tggcaaagga actagaacaa
    42961 ctgggtgcgc atatcattgc aatcaaagat atggctgggc tgcttaaacc gcaggcagct
    43021 tatcgtttga ttagtgaatt gaaagaaacg acagatctgc caatccatct gcatactcat
    43081 gataccagcg ggaatggaat catcacttat tctgctgctt caaaagcagg agtagacatc
    43141 gttgacgtgg caatgagtgc aatgagtggg aatactagtc agcctagcat gagcagcttg
    43201 tattatgcac tggtcaatgg tccacggctg ccagaaatca cgattgaaaa tgcacagaaa
    43261 ttaaaccatt attgggaaga tgtgcgcatg tattacaaac cgtttgaaaa cggcttgaat
    43321 gctcctgaaa cagaagtata tatgcatgaa atgccaggag gacagtattc gaatctacag
    43381 caacaggcaa aagctgtcgg attaggtcat cgctgggatg aaatcaaaca aatgtatcat
    43441 actgtcaact tgatgtttgg cgatatcgtt aaagtaactc catcatctaa agtagttgga
    43501 gatatggcac tatttatggt ccaaaatgat cttacagaag aagatattta tgaaaaaggc
    43561 gaaacactaa gtttccctga atctgttgtg actttcttcc aaggagaact agggcaaccc
    43621 gttggcggat ttcctaaaaa attgcaaaag attattctta aaggcagacc tgcattgaat
    43681 gaacgtccag gtcttttggc tgaatcagtc gacttcaacg aagtaaaaaa agaattggca
    43741 gaaaaaattg gctatgaacc aaaacaagac gaagtattaa gctatttgat gtatccgcaa
    43801 gtctttttgg actatcaaaa agcatataat caatttggcg atgttacatt actggacaca
    43861 ccaacatttt tccaaggtat acgtttgggt gaaacaatca atgtacaaat cgaacgcgga
    43921 aaaacgctta tcattcgttt agatgaaatc ggagaacccg atatcgaagg gaatcgcgta
    43981 ctgttcttca atttgaatgg acaaagacgt gaaatcacta tcaaagataa ttcgatcatc
    44041 agtacggtcc aaacccgatt gaaagctgaa ccgacaaacc gagaacaaat tggagcaacg
    44101 atgtctggtt ctgttttgga tgtattagtg aaaaaaggcg ataacgttaa aaaaggtgat
    44161 acgttgatga ttactgaagc gatgaaaatg gaaacggcca tcgaagcacg ttttgacgga
    44221 gaagtcgcac atgtatatgt gtcttcagga gacactattt cgtctggtga ccttttgatt
    44281 gaagtgactg agaaataata agcgaaaggt ggggaaacat gaaaagactg ggtttgtttt
    44341 ggctgattct gctattagtt attgtggcat tctacatgga accagtcttg tttccgccaa
    44401 aacttgaagt gaaggaaaac tctgatcaac aaatatcaaa ccaaatgaca acgtggaaat
    44461 accaggagct taaagcaact ggtttttcta cttatatcgg aaaaccgatc gatcagctgg
    44521 aaaaggattt tggccaagcc tatgatcgat taactagcgg atttggtttt gaaacaagat
    44581 attatgaaaa taaagctagt catctcacat ttgaagcgaa catccaagac agcagagtca
    44641 caactgttaa agtactgcaa acagggcctg ctgatatcgc gccgttttat ttagggatga
    44701 cgatgcaaga tcttacgaaa atcactacta tctatactaa tttcactttt gagtaccatg
    44761 atacagaagt tggaatcgaa ctaatggaag aggatatgaa ttatcgtccg ctaattgctt
    44821 ttgataacga cacttttgct attttatttt ttgatcaaac aacaaacggc ctattttcag
    44881 ttgtgtattt agataaagat agtcttttga aactattgcc atatcaagtg tttggtgaag
    44941 gccttccaca ttatcaagca gataaatcgg ctgattggga atcaatcaat cgcgttaaag
    45001 aacgaaaaag tacgacttta ttaaatatgc ttagaagaga agacgaactt cccatataca
    45061 atcaaacact tgcctttcaa aaccacagtc agctgctact acatgatttt ttgacaaggc
    45121 ctgaggaaat attgtcagag gaacgtatgg ctgattggca aaaatcgcta ggcagtgctc
    45181 aaacgacagt gaaattctca ctgacgaatg atgaattgga gaagttaatc agtaaacaaa
    45241 aattagaaaa tactagtggg ctatttattc atccggcgat tgacgctact ttttcgtttt
    45301 tatattggta cagcgatcct tacaaccatg accgtttcat ggacccggga acggattctg
    45361 taggcattgc tttttctaaa gaaaatatgt tagtcttatt gcaagaagaa aacggagaaa
    45421 gtagtgatag cagtgatcgt taacgaagag ctgtttcaac tggaggatca atgcctccaa
    45481 gtcgctgaaa tgataaaaaa gagtaaaacg atggcttctt atcttgaaca taaacagtca
    45541 atggaagaat ctgcaaaagt ttgccagttg aaagaagcgt tcttgtctaa gaaagaagca
    45601 tttgaaaatg tagcggcata tggtgagtat gcacctgatt atcgtgaaaa aaaacgagct
    45661 gttcgaaaag caaaaagaca gcttgatatg gatgagcatg ttgcagcttt ccgatatagt
    45721 gaaacacagc tgcaaagtct gttggatcaa attgtccaag tacttgccga agaagtttct
    45781 aatgaaatca aagtagatgc aggtagcccg ttttttgaaa cgaaaaaaca ctttggatgc
    45841 ggaggtaatt gtcatgcaat tagacaatag tgaatttgaa ataacaaaac gacgcgggct
    45901 gatcgtatgg gtctacagct taaaacaatt aaagaatctg aaaaagttcg gcttgttgca
    45961 ttatgtctca agaagaatga aatatgtcgt gctttactta aatgaagaga cattggaaga
    46021 aacagaagaa cgaatccgta aattacattt tgttcgaaac gtagaacgtt cttaccgacc
    46081 ggatatcgaa atgaattttg ccgaaaaaat aggaaacaaa acagtattaa aagacgaagg
    46141 tttcgaaatt gaagagctaa atacagaaat ccgattagct gaacatcttt aaaaatctct
    46201 tatgagcagt ttttaaataa aaaataactt ggataacgat cgatttttga tcattgtcca
    46261 agtatttttt tggtatacta gtaccagtac catttctaag gagtgagtag atgcggataa
    46321 tatcaggaaa ttatggcggg cgccgtttaa aaagtttagc aggggcgaac accagaccaa
    46381 ctacagataa agtaaaagaa tccatattca atatgattgg accatatttt gatggagaga
    46441 cagtattgga tctgtttgca ggaagcggtg gtttagctat cgaagcagta tctagaggct
    46501 gcagtcatgc ggtatgcgta gacaaaaatt accaagcgct aaaaatcatc aaagaaaaca
    46561 tcgaaattac gaaagaacca gagaaattta ccacaataaa acttgatgcg gacaaagcaa
    46621 tcgaacggct agcagcggag aaacaacaat tcgactactt gtttcttgat ccgccatatg
    46681 caaaacaaaa aataatcgat caaatccata aaatggaaga actgggactt ttttctaaaa
    46741 ccgcagttat cgtatgcgaa acggataaaa cagtcgaatt gccagaaact gtggctggat
    46801 tccatcaaat aagaaaacag atttatggca taacggccgt aactatctat agaaaagagg
    46861 gaaatcaatg aaacgtgcat tattcccagg aagttttgat ccttttacaa aaggtcactt
    46921 agatacggtg gaacgtgcgg ctaaattatt cgatgaggta gtaattggtg tttttatcaa
    46981 tacttcaaag aaaagtttgt ttcccccaga agaaagaatg acattgatca caaaagctgt
    47041 ttctcatctt cctaatgtaa aagtcatgca tcaagaaaat caattgacgg tagaaacagc
    47101 aaaagagatt ggtgcagatg cgttgatcag aggcatacga agtatcaaag actttgaata
    47161 tgaacgagag attgctcaga tgaatcatca tctatatgat gaattggaaa ctgtcttctt
    47221 attagcaaaa ccagaatata gtcacgtgag ttccagtatt ttaaaagaag tactgcactt
    47281 tggcggagat gtgagcagct acttaccgcc agtgatcaac gaagctttag ctggaaagcg
    47341 tgaatcaaat gaaagctaaa caatggttga aaacagtact gattatcata tttggaatgt
    47401 tgatcattgg atttgtagta gttcccttgc cgtattatgt agaatcaccg ggagccacga
    47461 tcgatctgaa agaactaatt acggtcaatg ataaggaaga taaaggttct gggtctttct
    47521 ctcttacctc tgttggtatc aggcaagcta caggtttctt agctttaaaa gctaaaatga
    47581 ccccttttga agaattgatc agtgaagaag aattgactgg tggggcgaca aatgaagaat
    47641 ataatcaaat gcaacagtat tatatggatt cttcccaaaa tgcagcaatc gaacaagcgt
    47701 taaaacttgc agacaagcct tataaaatgg aattcaaagg cgtatatgtc atgagtgtag
    47761 agaaaacatc gaatttcttt ggcaagcttt cagttggaga tacagtaaca aaagtagacg
    47821 ggaaaagctt tcaaagtaca gaagcattta tggattatgt aaaaagccaa aaagttggac
    47881 aaacaatcga aatcagttat ttgcataatg gtgaagaaaa aacagcttct ggtgatctga
    47941 tcgagttacc aacagataaa aaggcaggaa taggtatcac gcttactgac cataccgaga
    48001 tcgaatcaga tacagatgtt cgtattaatt ctggctctat cggtgggcca tcagcgggat
    48061 tgatgttcac tttagaaatc tatgaacaag tcactggtaa aaacttgcga catggtaagc
    48121 aaattgcagg aactggaacg atcaacagtg aaggagaagt tggccgaatc ggaggaatcg
    48181 ataaaaaagt ggcaagtgcg gataaagctg gcgtagagat tttctttgca ccagatgata
    48241 cgatttcaga cgatgttaag aaagaatatc ctgaaataaa aaccaactac gaagaagcaa
    48301 aagctgccgc taaaaaattg aattcctcaa tgaaaatcgt accagtaaaa acagtacaag
    48361 aagcgcttga ctatctagag aaaatgaaat gaaactaaat aattaaagag gggttgtgac
    48421 acgagttctg tcacaacccc tctttttcgt caaaa
//
LOCUS       DACESX010000004        44804 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.004, whole genome shotgun sequence.
ACCESSION   DACESX010000004 DACESX010000000
VERSION     DACESX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.004"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            212..604
                     /locus_tag="IXF88_000199"
     CDS             212..604
                     /locus_tag="IXF88_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1506960.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IXF88_000200"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IXF88_000200"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1506961.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IXF88_000201"
     CDS             3165..4775
                     /locus_tag="IXF88_000201"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1506962.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IXF88_000202"
     CDS             5067..5936
                     /locus_tag="IXF88_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1506963.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IXF88_000203"
     CDS             6101..7372
                     /locus_tag="IXF88_000203"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1506964.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IXF88_000204"
     CDS             7369..8664
                     /locus_tag="IXF88_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1506965.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IXF88_000205"
     CDS             8763..9026
                     /locus_tag="IXF88_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1506966.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IXF88_000206"
     CDS             9471..9920
                     /locus_tag="IXF88_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1506967.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IXF88_000207"
     CDS             9977..11395
                     /locus_tag="IXF88_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1506968.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IXF88_000208"
     CDS             11395..12255
                     /locus_tag="IXF88_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1506969.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IXF88_000209"
     CDS             12429..13202
                     /locus_tag="IXF88_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1506970.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IXF88_000210"
     CDS             13349..13714
                     /locus_tag="IXF88_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1506971.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IXF88_000211"
     CDS             complement(13862..15070)
                     /locus_tag="IXF88_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1506972.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IXF88_000212"
     CDS             complement(15089..15367)
                     /locus_tag="IXF88_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506973.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IXF88_000213"
     CDS             15560..16216
                     /locus_tag="IXF88_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1506974.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IXF88_000214"
     CDS             complement(16658..17455)
                     /locus_tag="IXF88_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1506975.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IXF88_000215"
     CDS             complement(17452..18636)
                     /locus_tag="IXF88_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1506976.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IXF88_000216"
     CDS             complement(18661..19908)
                     /locus_tag="IXF88_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1506977.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLL
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IXF88_000217"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IXF88_000217"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1506978.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IXF88_000218"
     CDS             21888..22202
                     /locus_tag="IXF88_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1506979.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IXF88_000219"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IXF88_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1506980.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IXF88_000220"
     CDS             22887..23531
                     /locus_tag="IXF88_000220"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1506981.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IXF88_000221"
     CDS             23546..23875
                     /locus_tag="IXF88_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1506982.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IXF88_000222"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IXF88_000222"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1506983.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IXF88_000223"
     CDS             24863..25687
                     /locus_tag="IXF88_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1506984.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IXF88_000224"
     CDS             25680..26027
                     /locus_tag="IXF88_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1506985.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IXF88_000225"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IXF88_000225"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1506986.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IXF88_000226"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IXF88_000226"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1506987.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IXF88_000227"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IXF88_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1506988.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IXF88_000228"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IXF88_000228"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1506989.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IXF88_000229"
     CDS             31684..32451
                     /locus_tag="IXF88_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1506990.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IXF88_000230"
     CDS             32441..34483
                     /locus_tag="IXF88_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1506991.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IXF88_000231"
     CDS             34764..34913
                     /locus_tag="IXF88_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1506992.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IXF88_000232"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IXF88_000232"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1506993.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IXF88_000233"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IXF88_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1506994.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IXF88_000234"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IXF88_000234"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1506995.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IXF88_000235"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IXF88_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1506996.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IXF88_000236"
     CDS             39330..40412
                     /locus_tag="IXF88_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1506997.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IXF88_000237"
     CDS             40412..41215
                     /locus_tag="IXF88_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1506998.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IXF88_000238"
     CDS             41285..42025
                     /locus_tag="IXF88_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1506999.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IXF88_000239"
     CDS             42036..43040
                     /locus_tag="IXF88_000239"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1507000.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44782)
                     /locus_tag="IXF88_000240"
     CDS             complement(43085..44782)
                     /locus_tag="IXF88_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507001.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14387 a         7822 c         9337 g        13258 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgcca gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacat
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaa
//
LOCUS       DACESX010000005        44740 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.005, whole genome shotgun sequence.
ACCESSION   DACESX010000005 DACESX010000000
VERSION     DACESX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.005"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..460
                     /locus_tag="IXF88_000241"
     CDS             <1..460
                     /locus_tag="IXF88_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,4-galactanase"
                     /protein_id="HAQ1507002.1"
                     /translation="QAGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAG
                     TFFGDYKLTFWQDQAYEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAE
                     LNTAVVKTSEWTKITIKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(951..2516)
                     /gene="guaA"
                     /locus_tag="IXF88_000242"
     CDS             complement(951..2516)
                     /gene="guaA"
                     /locus_tag="IXF88_000242"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1507003.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(2575..3498)
                     /locus_tag="IXF88_000243"
     CDS             complement(2575..3498)
                     /locus_tag="IXF88_000243"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1507004.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            3694..4299
                     /locus_tag="IXF88_000244"
     CDS             3694..4299
                     /locus_tag="IXF88_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1507005.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            4607..5623
                     /locus_tag="IXF88_000245"
     CDS             4607..5623
                     /locus_tag="IXF88_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1507006.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            5764..7065
                     /locus_tag="IXF88_000246"
     CDS             5764..7065
                     /locus_tag="IXF88_000246"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1507007.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            7111..7773
                     /gene="deoC"
                     /locus_tag="IXF88_000247"
     CDS             7111..7773
                     /gene="deoC"
                     /locus_tag="IXF88_000247"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1507008.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            7794..8192
                     /locus_tag="IXF88_000248"
     CDS             7794..8192
                     /locus_tag="IXF88_000248"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1507009.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            8258..9340
                     /locus_tag="IXF88_000249"
     CDS             8258..9340
                     /locus_tag="IXF88_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1507010.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            9552..11114
                     /locus_tag="IXF88_000250"
     CDS             9552..11114
                     /locus_tag="IXF88_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507011.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            11107..12240
                     /locus_tag="IXF88_000251"
     CDS             11107..12240
                     /locus_tag="IXF88_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1507012.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            12240..13196
                     /locus_tag="IXF88_000252"
     CDS             12240..13196
                     /locus_tag="IXF88_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1507013.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            13295..14461
                     /gene="deoB"
                     /locus_tag="IXF88_000253"
     CDS             13295..14461
                     /gene="deoB"
                     /locus_tag="IXF88_000253"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1507014.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            14485..15303
                     /locus_tag="IXF88_000254"
     CDS             14485..15303
                     /locus_tag="IXF88_000254"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1507015.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            15320..16027
                     /gene="deoD"
                     /locus_tag="IXF88_000255"
     CDS             15320..16027
                     /gene="deoD"
                     /locus_tag="IXF88_000255"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1507016.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(16325..17011)
                     /gene="gpmA"
                     /locus_tag="IXF88_000256"
     CDS             complement(16325..17011)
                     /gene="gpmA"
                     /locus_tag="IXF88_000256"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1507017.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(17183..17764)
                     /locus_tag="IXF88_000257"
     CDS             complement(17183..17764)
                     /locus_tag="IXF88_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1507018.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(17897..18580)
                     /gene="rpiA"
                     /locus_tag="IXF88_000258"
     CDS             complement(17897..18580)
                     /gene="rpiA"
                     /locus_tag="IXF88_000258"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1507019.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            18899..19312
                     /gene="rpsL"
                     /locus_tag="IXF88_000259"
     CDS             18899..19312
                     /gene="rpsL"
                     /locus_tag="IXF88_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1507020.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            19396..19866
                     /gene="rpsG"
                     /locus_tag="IXF88_000260"
     CDS             19396..19866
                     /gene="rpsG"
                     /locus_tag="IXF88_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1507021.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            19960..22047
                     /gene="fusA"
                     /locus_tag="IXF88_000261"
     CDS             19960..22047
                     /gene="fusA"
                     /locus_tag="IXF88_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1507022.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            22219..23406
                     /gene="tuf"
                     /locus_tag="IXF88_000262"
     CDS             22219..23406
                     /gene="tuf"
                     /locus_tag="IXF88_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1507023.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            23813..24121
                     /gene="rpsJ"
                     /locus_tag="IXF88_000263"
     CDS             23813..24121
                     /gene="rpsJ"
                     /locus_tag="IXF88_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1507024.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            24152..24781
                     /gene="rplC"
                     /locus_tag="IXF88_000264"
     CDS             24152..24781
                     /gene="rplC"
                     /locus_tag="IXF88_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1507025.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            24809..25432
                     /gene="rplD"
                     /locus_tag="IXF88_000265"
     CDS             24809..25432
                     /gene="rplD"
                     /locus_tag="IXF88_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1507026.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            25432..25722
                     /gene="rplW"
                     /locus_tag="IXF88_000266"
     CDS             25432..25722
                     /gene="rplW"
                     /locus_tag="IXF88_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1507027.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            25761..26594
                     /gene="rplB"
                     /locus_tag="IXF88_000267"
     CDS             25761..26594
                     /gene="rplB"
                     /locus_tag="IXF88_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1507028.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            26637..26915
                     /gene="rpsS"
                     /locus_tag="IXF88_000268"
     CDS             26637..26915
                     /gene="rpsS"
                     /locus_tag="IXF88_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1507029.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            26938..27285
                     /gene="rplV"
                     /locus_tag="IXF88_000269"
     CDS             26938..27285
                     /gene="rplV"
                     /locus_tag="IXF88_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1507030.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            27299..27955
                     /gene="rpsC"
                     /locus_tag="IXF88_000270"
     CDS             27299..27955
                     /gene="rpsC"
                     /locus_tag="IXF88_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1507031.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            27958..28392
                     /gene="rplP"
                     /locus_tag="IXF88_000271"
     CDS             27958..28392
                     /gene="rplP"
                     /locus_tag="IXF88_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1507032.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            28382..28570
                     /gene="rpmC"
                     /locus_tag="IXF88_000272"
     CDS             28382..28570
                     /gene="rpmC"
                     /locus_tag="IXF88_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1507033.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            28595..28861
                     /gene="rpsQ"
                     /locus_tag="IXF88_000273"
     CDS             28595..28861
                     /gene="rpsQ"
                     /locus_tag="IXF88_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1507034.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            28919..29287
                     /gene="rplN"
                     /locus_tag="IXF88_000274"
     CDS             28919..29287
                     /gene="rplN"
                     /locus_tag="IXF88_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1507035.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSHIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            29324..29632
                     /gene="rplX"
                     /locus_tag="IXF88_000275"
     CDS             29324..29632
                     /gene="rplX"
                     /locus_tag="IXF88_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1507036.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            29660..30199
                     /gene="rplE"
                     /locus_tag="IXF88_000276"
     CDS             29660..30199
                     /gene="rplE"
                     /locus_tag="IXF88_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1507037.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            30218..30403
                     /locus_tag="IXF88_000277"
     CDS             30218..30403
                     /locus_tag="IXF88_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1507038.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            30439..30837
                     /gene="rpsH"
                     /locus_tag="IXF88_000278"
     CDS             30439..30837
                     /gene="rpsH"
                     /locus_tag="IXF88_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1507039.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            30869..31405
                     /gene="rplF"
                     /locus_tag="IXF88_000279"
     CDS             30869..31405
                     /gene="rplF"
                     /locus_tag="IXF88_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1507040.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            31566..31922
                     /gene="rplR"
                     /locus_tag="IXF88_000280"
     CDS             31566..31922
                     /gene="rplR"
                     /locus_tag="IXF88_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1507041.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            31943..32443
                     /gene="rpsE"
                     /locus_tag="IXF88_000281"
     CDS             31943..32443
                     /gene="rpsE"
                     /locus_tag="IXF88_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1507042.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            32458..32637
                     /gene="rpmD"
                     /locus_tag="IXF88_000282"
     CDS             32458..32637
                     /gene="rpmD"
                     /locus_tag="IXF88_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1507043.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            32681..33121
                     /gene="rplO"
                     /locus_tag="IXF88_000283"
     CDS             32681..33121
                     /gene="rplO"
                     /locus_tag="IXF88_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1507044.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            33121..34416
                     /gene="secY"
                     /locus_tag="IXF88_000284"
     CDS             33121..34416
                     /gene="secY"
                     /locus_tag="IXF88_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1507045.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            34478..35125
                     /locus_tag="IXF88_000285"
     CDS             34478..35125
                     /locus_tag="IXF88_000285"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1507046.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            35316..35534
                     /gene="infA"
                     /locus_tag="IXF88_000286"
     CDS             35316..35534
                     /gene="infA"
                     /locus_tag="IXF88_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1507047.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            35567..35683
                     /gene="rpmJ"
                     /locus_tag="IXF88_000287"
     CDS             35567..35683
                     /gene="rpmJ"
                     /locus_tag="IXF88_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1507048.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            35701..36066
                     /gene="rpsM"
                     /locus_tag="IXF88_000288"
     CDS             35701..36066
                     /gene="rpsM"
                     /locus_tag="IXF88_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1507049.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            36094..36483
                     /gene="rpsK"
                     /locus_tag="IXF88_000289"
     CDS             36094..36483
                     /gene="rpsK"
                     /locus_tag="IXF88_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1507050.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            36563..37501
                     /locus_tag="IXF88_000290"
     CDS             36563..37501
                     /locus_tag="IXF88_000290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1507051.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            37529..37909
                     /gene="rplQ"
                     /locus_tag="IXF88_000291"
     CDS             37529..37909
                     /gene="rplQ"
                     /locus_tag="IXF88_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1507052.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            38181..39020
                     /locus_tag="IXF88_000292"
     CDS             38181..39020
                     /locus_tag="IXF88_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1507053.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            38996..39865
                     /locus_tag="IXF88_000293"
     CDS             38996..39865
                     /locus_tag="IXF88_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1507054.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            39855..40652
                     /locus_tag="IXF88_000294"
     CDS             39855..40652
                     /locus_tag="IXF88_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1507055.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            40855..41601
                     /gene="truA"
                     /locus_tag="IXF88_000295"
     CDS             40855..41601
                     /gene="truA"
                     /locus_tag="IXF88_000295"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1507056.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(42056..44725)
                     /locus_tag="IXF88_000296"
     CDS             complement(42056..44725)
                     /locus_tag="IXF88_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1507057.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        14344 a         8288 c         9706 g        12402 t
ORIGIN      
        1 acaagcaggg gcgcaaataa atctagttaa aaatgcaagt ttcgaaagcg atggaccaac
       61 gaccagtcct tccaattggc aaacctggtt tcaacaagga accgctaagg gtacgatcaa
      121 aacggaagcg ggcacttttt ttggggacta caaactaact ttttggcagg atcaagcata
      181 cgaggcatca gcttatcaaa atgttagtgg cataagcaaa ggaacttaca cattgagtgc
      241 ctatgtaatg tctgggggag agtttgatac taatcagtta tatatcaaaa attatggtgg
      301 agcggaacta aatactgcgg ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga
      361 cattccaatt acaacaggtg tttgtgaaat tggggtttat agcaaggctt caggaaatgc
      421 ttggacgaac gttgattttg tttcattggt gaaacaatag actgcggatc gtttcgattc
      481 aagatcggag gttcccttca gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac
      541 ctcgcctttg ccaaaaataa ccagaaacga tttccagtca tttttaccaa aacgagtaaa
      601 gcaccagact tcatctacac tttgtattat gctaaagaga gttcaacgat ttcgtaagaa
      661 gcaatgaaaa aattgcaaaa agttgtgcag ataacataaa gtttgtttaa gaaagggtaa
      721 ccttagaatt ccgtcgtgaa tatgatttga gcacttagat ggatagaaaa aagttctcgg
      781 aaaacagttc gtttttcgag aacttctttt tatgataaga atgaaacaaa ttaacttata
      841 ttatagtctc ttttggtagg gaaccatctt atttttaatg aaaatcaagc attttagcta
      901 ttgaagaatg ttgatttata gggattcccc ctccatatta tgttggctct ttattcccac
      961 tccactgttg ctggtggttt agaagtgatg tcatacacga tacggttgac gtgggctact
     1021 tcgtttacga tacgtacaga gattttttgc aagacttccc atgggatacg ggcaaagtca
     1081 gcggtcattc cgtcgattga agttacagca cggataccga ctgtgtagtc gtaagtacgt
     1141 ccgtcaccca taacaccgac agagcggatc cctggaagaa cagtgaaata ttgccagata
     1201 tcacggtcaa gtccagcagc tgcgatttct tcacgcaaga tagcatcaga ttcgcgtacg
     1261 atttctagct tgtcttcagt gatttcgcca agaacacgaa taccaagacc aggaccaggg
     1321 aatggctgac gccaaacgat tgaatcaggc atgcctaact gagtccctaa ttcacggact
     1381 tcgtctttga ataatgtgtt taatggttcg atcaattcaa attgcatatc ttctggcagt
     1441 ccacctacat tatggtgaga tttgatcgtc tgtgctgttt ctgtaccaga ttcgataacg
     1501 tcagtataca acgttccttg tgctaggaaa gagacgcctt cttcaccagc aagttttgtt
     1561 gcttcgtcat caaaaacata tacgaattca ttcccaatga ttttacgttt ttgttctggg
     1621 tcagatacac ctttcaactt gtctaagaag cgatctcttg cattgacttt gatgatgttc
     1681 aagccgaatt ttccgcctag actttccatt acttgttccg cttcgccttt gcgtagcaga
     1741 ccgtggtcaa caaagataga tgtcaattgg tcaccgatcg ctttttgtaa aagaacccca
     1801 acgacactag aatcaacgcc acctgaaaga ccaagcaaga cttttttgtc accaacttgt
     1861 tcacggattt tttctacttg catatcgata aagttatcca ttgtccagtt accttcacag
     1921 tgacaaacat caaaggcaaa gtggcgcagc aattcgattc cgtattcgga atgacgtact
     1981 tctgcatgga attggatacc ataaaaatta cgtgttgtgt cttggataga agcaatcgga
     2041 caatctttgc ttgttccgac tgtttcaaag ccttcaggaa ctttagttac caagtcacca
     2101 tggctcatcc atactgtttg ttgttttggt gtttctttaa acaagacagc ttgatcgctt
     2161 gttacttcta acaaggcttt cccatattcg cggtttttag caggctctac ggaaccgcca
     2221 agtttgtacg tgatcaattg cataccatag caaatcccta atacaggaat acctaaattg
     2281 aagatctcag gatcgatccc aaatgcattc tcatcatata cactgtttgg tccgccagaa
     2341 agaatgattc cctttggatt catcgctttt acttcttctg cagtaatgcg gtggctcaat
     2401 aattcggaaa agactccaag atcacgaata cgtcttgtga tcaattggtt atactgacta
     2461 ccgtagtcta atacgataat cttttcaacg ttcgtcatat cggcaacgtt tgtcaaattt
     2521 attaccccta tccctttaaa ttttatctca aaatagtcaa actattttaa aatgctaata
     2581 ttttcgcatg aaaatcgaat cgatgatatg attttcagtt ttatggagaa ttatatcggc
     2641 tcttcctcgt gtaggcaaga tatattcctg taaatttttc aaattcacat ttttccatac
     2701 atcttttgcc attttaaagg cttcttcacg atctccgata gcaaaacgat aataataatt
     2761 ctctggattt tggaaagccg tatccagtaa cgcaccaaaa cgatccaaat accatttttc
     2821 gatcagcacg ggatcggcat cgacatagac agaaaaatca aaaaagtcgc tgacgtagat
     2881 ttgctgattc gcaggtaatt gaagcgtatt gatcccctca acgatcaaga tatcaggctg
     2941 ctggatcaat tcgtacttgt ctggaatgat atcatacaca tcatgtgagt aaacaggtac
     3001 ttttatctct tcctgaccag attttacacg gttcaagaaa tcgatcagca tctccatatc
     3061 ataactttca ggaaatcctt tacggtccat gattccacgc tcttttaata tacgatttgg
     3121 ataaagaaat ccgtcagtcg tgatcagctg aacgtttctt cgtttgaagg tacgagacaa
     3181 aatcgtctgc agcagtctgg ctgttgtact ttttccaacg gctacacttc cggcgatccc
     3241 gataataaaa ggcggtactt ctacgtattt atgaagaaat aaacctttac tcaagaacaa
     3301 agattcaaat tctttcatat aaagataaat cagatgagca agaggtacat agatatctcg
     3361 aacatcctgc atggaaatgc ggtcattcaa acttttgata ttatctagtt catcttgtgt
     3421 caaaggtgct tgcccattac gataaaattc tcgccattct tctcttgcca cacggtagta
     3481 gttcattgga tcattcatat tagcctcctg cgttctccca tcaatggaac tattttaaca
     3541 caaaataaac gcaaacccca ccacttaaga gagattgccg atctttttgt ataatcttta
     3601 taatagaaag aaacggagtc ttattcttga cttgcgcacg gatacataaa aggatagact
     3661 gttgtcatga aagaaatgag aaggatggaa gacatgacaa atcattacta ttctgaacgc
     3721 ccagatacgg cgcacgattt tgaacaatgg gcttttgagt taaaaggcag aacctttcaa
     3781 tttgtaacag attctggtgt attttcacgt gaaacagtgg attatggttc acgtgtcctg
     3841 attgatgctt tcgattggga agagttacct gatgaaggaa agatcttaga tgtgggatgc
     3901 gggtatggac cgattgggtt agctttggca tttgcgacac agcggtttgt tgaaatggtc
     3961 gacattaatg aacgagcggt aagtctagcg caaggaaacg caaagcgtaa ccaaatcgaa
     4021 catgttgata tccatcaatc gaatatctat gaagcagttc atgaagaaac ctatgcagct
     4081 atcgtaagca atccgccgat ccgtgctgga aaaaaagttg tacatgaaat tttgacaggc
     4141 gcatatcctc ggttgaagaa gggcggtaca ctgacaattg tgatccagaa gaaacaagga
     4201 gcgccgagtg cgcaaaagaa aatggaagaa acatttggta atgcagaaat cgtcacaaaa
     4261 gataaaggat attatatcat caaaagtgtg aaagaataac tagctgaaaa atagtggagc
     4321 gacgaaagag tggaactggg cgtgcatgta cgggtacgta tcagttcttt ttttgttgag
     4381 aagtttttaa aaacaaagaa ctattttttc ttcaaaaaga aatagtagga acagagtcgt
     4441 tgttttttac gataaaataa ataagataca gaacttgtat agaaggctgc caagtcggta
     4501 gataaaggag gtttttatca aaaaacatac tttccttttt tctctattct tttcttgggg
     4561 gttttggtac actgataggg aatttagata gaagggagcc aataggatga agctgaccat
     4621 cagagatatc gcagaaatgg caggcgtatc agtaacaact gtctcacaga ttttgaataa
     4681 caaaggaagc agatttagtg ataagacaag aaaaaaagtg ttgtcgatcg tgaatgaaca
     4741 tcattataag cccgattatt tcgcttcaaa tgtgatcaat cgacattcaa agaccatcgg
     4801 aatgatcgtc cctgatgtca ctgatttctt cttctccaaa ctgatcgaag gtgtagagag
     4861 ctatttgaat cctttgggct atgtgatcat gttatgcaat tctcgtcata gccaagaaaa
     4921 tgagatcaag tatttacaag agctttccca ccgctcagtg gacggaatct tgctggccac
     4981 accaaatatt ttacctgaga aatatgcatt agacagcggt ttttatcaaa aaatgccaat
     5041 cattttaatc gatcgcggat tgaatcgtcg ggataacgga cggcttatcg taaaggaata
     5101 tgagggcgcc tatcaagctg tttcgctgtt tattgaaaat ggccatacga agatcggcat
     5161 gctgaaggaa accactggat actatcaatt agaagaacgt ttcaacggat atcgtcatgc
     5221 gctgaaagat catggcctgc cttttaatgg gaaatttgtt gaacatggag agttgactgt
     5281 ccaaggtgga tatactgcat caaaaaaatt actcaaacat aaagatatca cggctatttt
     5341 ttgcgggaat gatgccatgg cgatcggatg ctatcaggcg atcgatgaat tgggcaaaaa
     5401 aatcccagaa gatatctcag tcataggatt cgatggatta aagctgtcag aatacatgat
     5461 tccgcaattg acgacagtgc agcagccaag ttttgatatt ggattttatg ctgcacgttt
     5521 cctgatcgat acgatcgaat ttcctcaaag aaaagttccg aacaaagtgt tcgaaacaaa
     5581 attaattatt cgtgagagcg taaaagtatt gacaaagggc taactcggcg gtatattgct
     5641 tatgtaagca tttacaaagg ctagttagtg ctttttttat ttttagtaaa ccagtttact
     5701 aaagtggttt acctttccta aaaataatgt ataataccga atgaagacaa ggaggatcag
     5761 gcaatgcgaa tggtggatct gattgaaaaa aagagggatg gaaacgagct gtcgaaagag
     5821 gaaatcgagt acatcgtcac gaactatacg aatggaaaga ttccagatta tcaagtcagt
     5881 gcattattga tggcaatttt ttatcaagac atgacaaatg aagaaatcac gaacttaaca
     5941 ttggcgattg ctaattctgg tgatgtcatc gatttgagtt ctcttgaagg aataaaggta
     6001 gataagcatt cgacaggtgg cgttggtgac acgaccacat tgattttagc ccccctagta
     6061 gctagtgtcg gagtgacagt ggctaaaatg tctggacgcg gtctaggtta cacaggtggt
     6121 acactagaca agctagaagc aattcccgga tttcagatcg agctttcgga tgaagaattt
     6181 gtacgcattg ttaatgaaag taaagtagcg gtaattggtc aatcaggaaa cttggcaccg
     6241 gcggacaaaa aattgtatgc cttgcgtgac gtcacagcga ccgttgattc tcttccgctg
     6301 attgccagtt cgattatgag taaaaaaatc gcggcaggag cagatgcaat cgttttggat
     6361 gtgaccactg gagacggtgc tttcatgaag aacatcgaag atgcaagacg tctggcaaag
     6421 accatgacga gcattgggaa actggctaac cgagaaacag tggcggttat ctcagatatg
     6481 tcagaacctc ttggcgaagc tatcggaaat agtctagaag ttgtcgaggc aatcgaaaca
     6541 ttacaaggaa atggtccaga agatctagta gaaatgtgct acgccttagg aagccaaatg
     6601 gttgttcttg ctggcaaagc taagacaacc gatgaagcaa gaacgttgct tcaagaagct
     6661 ctggaatcag gtaaagcgtt agcaaaattc aaagaaatga tccaaaatca agggggagac
     6721 ccagcgatcg tggaacatcc agaacgaatt ttgactgcac gctacacaat ggagcttcct
     6781 gctaaacaaa gcggagttgt gtcaaaaatc gtggcaaatg aattaggaat tgctgcaatg
     6841 atgctaggtg ctgggcgtaa aaccaaagaa gaagatattg atcacgctgt tggattgaaa
     6901 ctgcataaaa aaattggtga cactgtgaca aaaggtgagt cactattgac gatctatagt
     6961 aacgacgaag aaatcacttc tgtcattgaa ttattatata aaaatattga aattggtgaa
     7021 tcggcaatga agccaacgtt gattcatgac atcatcacag aataaaggaa aaaagcggac
     7081 tttaccgttt taaagaaaag gggaaaagaa atggaactaa accgaatgat cgaccatact
     7141 attttgaaag cagacgcatc taaagaagct gtgatgcaaa ttatcgaaga agcaaaaaaa
     7201 taccattttt attctgtctg catcaatcca gcatgggttg cgctagcgaa agaacaatta
     7261 caaggtgagc cagtagccgt atgtacagta atcgggtttc ctctaggagc aaacacaccc
     7321 gagacaaaag catttgaaac aacagatgcc atcaataacg gtgcagatga agtcgatatg
     7381 gtcatcaata tcggtgcttt gaaatcaaag gatgatcaac aagtccaaaa agatatcgaa
     7441 gcagttgttg aagcagcaaa agatcgtgcg cttgtcaaag ttattattga aacatcgtta
     7501 ttagatagag aagaaatcat ccgagcttgc gaaatagcaa aagctgcagg cgctgatttt
     7561 gtcaaaacat caactggttt ctcaacaggc ggagcaaagg tagaggatgt ccgtttgatg
     7621 cgtgaaactg taggaccaga aatgggtgtc aaagcttccg gcggtatcca taacgaagaa
     7681 gaagcaatgg caatgatcga agctggtgct acaagaatcg gaacaagcgc aggagtagca
     7741 atcgtatctg gttcaactgg tgaaggctat taattttttc aaaggagaag ctcatggcag
     7801 aactcaaaca agaatggatc aacacagcta ttgaagcgtt agataaagcc tatgttcctt
     7861 attcccattt tccggtaggc gcttgtctcg taacggaatc aggcaagatt tatcaaggca
     7921 tcaacattga gaatgcatca tttggattaa cgaattgtgc cgagcgcaca gcttttttca
     7981 aagcagtatc tgaaggagaa aaaacattta cgcatctagt cgtagcgggg cacacccccg
     8041 atcctatctc tccttgtggt gcatgtcgac aagtaatggc agaattctgt tcccctgata
     8101 tgccggttac gctagtagga gacaatggtg tgacgaaggc aacgaccgtt cgtgcactgc
     8161 tgccttatgc atttactgaa aaagatctat aactcatgtt cattcagtca gctgactgtt
     8221 gaaaaataaa acaattattt taggaggctt tacagaaatg aaaaaagcaa aattatttgg
     8281 tttaggtgct gtcgcactag cagcagggtt atttttaggt gcatgcggca acaacggctc
     8341 tactgattca tccggtggca aaagctcatc agacacaaca actgcagcat tgatcacaga
     8401 cacaggtggt gtagacgacc gttcattcaa ccagtcagct tgggaaggac tagaaaaatg
     8461 ggggaaagat caaggacttt cacgaggaaa tgatggcttc caatatttcc aatcttcaaa
     8521 tgaatcagat tacatcccaa atattgacca agcattgaat gctggattca aaacgatttt
     8581 tggtattggt tataaactaa aaccagcgat ccaagaacaa gcaacaaaca atacaggtac
     8641 gaactttgtt attatcgatg atgtcatcga tggattagat aacgttgtat ctgcaacttt
     8701 caaagacaac gaagcttctt atcttgcagg tgttgcagca gcttatacga ctgaaacaaa
     8761 cgtagtcggc tttatcggcg gtgtaaaagg tgaagtaatc gatcgttttg atgctggatt
     8821 caaagccggt gttgatgcag gtgcgaaaga attaggaaaa gaaatcaaag tattgaatca
     8881 atatgctggt gacttctctg ctccagataa aggacgttca attgctcaag gtatgtatgc
     8941 acaaaacgca gatatcatct tccatgcttc aggcggtaca ggtaacggtg tattccaaga
     9001 agcaaaatca ttgaacgaat caggcgacaa aaaagtttgg gttatcggtg ttgaccgcga
     9061 ccaatcagac gaaggcgaat acacattaaa cggcgaaaag aaaaacttca cattgacatc
     9121 tacactgaaa gcagttggaa cagtagtaga agatttggca caaaaatcag cggacggtaa
     9181 attccctggc ggcgaacaca ctgtttatgg attgaaagaa gacggtgttg gtctaacaga
     9241 aggacaactt tctgacgaag cgaaaaaagc agttgatgaa gcaaaagaaa aaatcatttc
     9301 aggtgacgta aaagttccag aaactcctga agaaaactaa aaccatttct atagccttgt
     9361 tagacacatg tttgacaagg ctatggtttg attagaacaa gaattgttca tttatccaag
     9421 ctgccttgtg acagtttgga taagggaaca actccctgaa gtaatgtttg tttcaaaaga
     9481 agaacgctca ctaagcgtga atcttagtca atcgttgccc tagatagaaa tcgttaggat
     9541 gtgaaaaaaa ggtggttcaa gaaaatattg tcatagaaat gcagaatatt accaaagaat
     9601 ttggaacatt caaggcaaat gacaacatca atttacaagt aaaggcagga gaaattcatg
     9661 cgttgcttgg agaaaacggt gctggcaaat ctacattgat gaacgtgctt tccggattat
     9721 tagagccgac atcagggaaa attttgatgc gtgggaaaga agtaaagatc acaagtccga
     9781 ccaaagccaa ccaattaggg attgggatgg tccatcagca ctttatgctt gttgatgcct
     9841 ttactgtaac agaaaacatc gtgttgggaa gcgagcctag tcgcgcaggg atgcttgacc
     9901 ataaaaaagc acgaaaagag atccaaaaag tttctgaaca gtatggattg tcagtcaacc
     9961 cagatgctta cgttcgtgat atttcagttg gaatggaaca acgggtagaa attttaaaaa
    10021 cactttaccg aggagcagat gtactgattt ttgatgagcc gacagctgta ttgacccctc
    10081 aagaaattga tgaattaatc gtgatcatga aggaattagt caaagaaggc aagtcgatca
    10141 ttttgattac gcataagtta gatgaaatca aagcagtagc tgaccgctgt acagttatcc
    10201 gccgtggaaa aggaatcggt acagtcaacg ttaaagacgt aacctcacaa caattagccg
    10261 atatgatggt tggaagaacg gtttcattca aaacgatgaa aaaagaagcg aagcctcaag
    10321 aagtcgtttt gtctattgaa aatctagtgg tgaaagaaaa tcgtggatta gaagccgtga
    10381 aaaacttgaa cttagaggtt cgtgctggcg aagtacttgg tatcgctgga atcgatggaa
    10441 acgggcagtc ggagttgatc caagctttga ctggtttgcg aaaggcagaa agcggacata
    10501 tcaagctaaa aggggaagac atcactaata aaaaaccccg aaaaatcact gaacatggtg
    10561 taggacacgt gccagaagac cgtcataaat acgggttagt cctagatatg acattatctg
    10621 aaaatatcgc actacaaacc tatcatcaaa aaccttacag taaaaacggc atgctgaatt
    10681 attcagtaat gaatgaacat gccagagaat tgatcgaaga atatgatgtt cgaacaacga
    10741 atgaacttgt tcccgcaaaa gctttatcag gcggaaatca gcaaaaagca attatcgctc
    10801 gtgaagtcga ccgagatcct gatctgttga ttgttgcaaa tccaactcgt ggactggatg
    10861 taggtgcgat cgaatttatt cataaacgtc tgatcgaaca aagggacaaa tacaaagcag
    10921 tgttactgat cagtttcgaa ttagaagaaa ttttaaatgt ttcggatcgt attgctgtta
    10981 tccatgaagg agaaattgtc ggtatcgttg atccgaaaga aacatctgaa aatgaattag
    11041 gtctattgat ggcgggctat accttagaag aagcacgaaa agagttagaa aagggggctg
    11101 gcgcacatga atgatcgcag cgaaaaattc cgtaatttat tagtgcctgt actttcagtt
    11161 gttttaggat tgatcttagg cgcgatattg atggctgctt ttggttttca gcccatccaa
    11221 ggttattctt ctatgctaga tgcagccctt ggttcgcaaa gagggatagg tgaaacgtta
    11281 aggcaagcaa cgccgttgat ttttacagca ttaggttttt ctgtagctaa ctcagctggg
    11341 tttttcaata ttgggctttc tggacaagca ctttgcgggt ggatcgtcag tgtctgggtg
    11401 gcacttgcgt ttccagatct gccaaaagtt gttttgcttc cgttatgcgt gatcttaggt
    11461 gccttagctg gtgcgatggc agcggctgtt ccaggtttac ttcgagcatt ttttggaaca
    11521 agtgaagtga tcgtaacaat catgatgaac tatatcttat tatacgtaag cacatttgct
    11581 ttacatgaag tgatgcctga aagctatcgc tcaagtttgg actcttccaa tatgatcagt
    11641 caaaatgcct cactgcggtt accatggtta acacaagctt ttggcggctc tcgcgtaaac
    11701 tcaggattat ttttagcaat catcgcgctt attttcgttt ggctgctgat gaagaaaacg
    11761 actttaggat ttgaaatccg ttctgttgga ttgaatcctt ttgcttctga gtatgcagga
    11821 atgagtagta aacgtacgat cgttttgtcg atggtcattt ctggcgcttt ggcagggctt
    11881 ggcggtgtgg ttgaaggatt aggaacgtat caaaacttct ttgttcaaac gacttcgcta
    11941 tctattggat ttgacggaat ggctgtttcc ttgcttggtt ctggatcagc aatcggtatt
    12001 cttttatcag cgatcttgtt cagtattttg aagatcggtg gacttggcat gcagacagga
    12061 gcaggtgttc cgtttgagat cgtcaatgta tcgattgctt tgattatctt ctttgtcggg
    12121 atcaacttcc tgattcgttt cgtcatggct aaagtcttcc aagggaaaaa acaagaagag
    12181 atcgttgcga caatagaatc gaaaccaacg aatcaaaacc atgaaggggg cgaactataa
    12241 tgtcgactat cgaattaatt gccttgatcc ttacttctac actggtttat tcaacaccgc
    12301 taattctaac gtcactaggc ggaacttttt ctgaacgaag cgggatcgta aacgtagggc
    12361 tagagggaat catggtcatg ggtgctttta gttccatcgt tttcaacttg acgatgagtc
    12421 catcacttgg ttctatgaca ccatggatcg ctattttagt gggtggcgtc gtaggtatcc
    12481 tattttctct gattcacgca gtagcaacag tcagtttgcg tgctgaccat attatcagcg
    12541 ggacagtaat taatctgatg gcacctgctt taggtgtttt cttgatcaaa gcatggtatg
    12601 gtaaaggtca aactgataac atcacgcaga atctggggta tttttctttc ccaggattag
    12661 cagatattcc ggtcattgga ccaattttct ttaaaaatac attcttacct gcttattgtg
    12721 ctattttgat tgctgtccta gcttgggtga tcatcttcaa gacgaaattc ggcttgcgcc
    12781 tccgttcagt tggtgaaaat cctcaagctg ccgacacatt aggtatcaat gtatatgcta
    12841 tgcgttattc aggcgtactg atttctggct tgttaggcgg tatgggcggt gctgtttttg
    12901 ctcagtcgat ttccggtaac ttttcagctg gtacgatcgt tggacaaggg ttcatctcca
    12961 tggctgctat gatctttggt aagtggaatc ctttaggggc aatgggtgcc tcattgttct
    13021 ttggttttgc tcaaagcttg agtatcattg gcgcacaatt accagttatt tcttcgattc
    13081 cgccagtatt gctgcaaatt gctccatatt tgctgacaat cattgtgctg gttgttttct
    13141 tagggaaagc ttctggtcca aaagcaaacg ggaagaacta tatcaaatca aaataatctt
    13201 ttagcgaata aaaagggtgg gttacgggaa gtgtttttcg tctcgccctt tttgttttaa
    13261 aggcaataaa aataaaaaga aagaaggaat aatcatgttt aaacgcgtac atctgatcgt
    13321 catggattca gtaggaattg gagaagcacc agatgccgat aagttcggtg atgttggttc
    13381 tgatacatta ggacacattg cgaaagaagc aggattgaac gttccacatt tagagcagct
    13441 tggtttagga acgatccgtc ctcttgaagg tgtggaatca gtgaaagatc atcaaggcta
    13501 tgcgacgaaa ttagaagaag tttctgtagg gaaagatacg atgacaggcc actgggaaat
    13561 catgggattg aatatcaaaa ctccatttcg tgtgtttcct aatggattcc ctgatgatct
    13621 gatcaaacaa atcgaagaat tttctggccg taaagtcgta tgtaacaaac cttatagcgg
    13681 aacagctgtt attgacgatt acggtgaaca ccaaatgaaa acaggcgatt taattgtata
    13741 tacttctgcc gatcctgtcc ttcagatcgc ggcacatgaa gaaatcattc ctttagaaga
    13801 attatatcgg atttgccaat tcgttcgtga tataacaaaa gatgatccgt atatgattgg
    13861 ccgaatcatt gcacgtccgt acgtgggtga accaggagac ttcacacgaa caagcaatcg
    13921 ccatgattat gcgttgaatc cattcggaca cacagtcctt gattctttga aagaagcagg
    13981 aaaggacgtg attgctgttg gaaagatcaa cgatattttc aacggacaag gaatcactga
    14041 atctgtccgc acgaaaagca atatggatgg cgttgaccag ctgctgaaag ttatgaaaaa
    14101 cgatttttca ggaatcagct ttaccaattt agtagatttt gatgcattat acggtcatcg
    14161 tcgggatgta gtcggctatg cgcatgcgat tgaagaattt gacttacgaa ttccagaatt
    14221 gcttgaagcg atggaagaag acgacttgct aatgattaca gccgatcacg gaaacgatcc
    14281 gacgtttact ggtaccgatc atacgcgaga atatgtaccg ttattggttt acagtaaaaa
    14341 aatgaaagaa caaggggcat tgcctcaagg atattattca gatatttcag caacgattgc
    14401 tgagaacttt agtgtttctt cgactgaaaa tggcgaaagc ttcttgaaat tattaaaata
    14461 atctttttaa ataggagaaa gcaaatgacc aagttaagcg agatgttaca agaaacgacc
    14521 cagtttatcc ttgataaagg agtaaaagaa attgattttg gtttgattct aggttcagga
    14581 ttaggtgaat tagcagatga aatcgaagat gctgtaatga tttcttatga tgagatcccg
    14641 ttctttcctg tttcgacagt agttgggcat gcaggaaagc tagtttatgg gtctctggca
    14701 ggcaagaaag tgttagccat gcaagggaga ttccactact atgaaggtca ttccatgcaa
    14761 acagtgacct atccagtacg tgtgatggcc ggactagggg cccattcagt cattgtgact
    14821 aatgcatgtg gtggtgtcaa cgaatcattt gtaccaggag atttgatgct gattaccgat
    14881 catatcaatt ttactggtga aaatcctttg atcggtccga atgaagatgc aatgggccct
    14941 agatttccag atatgagtca agcttatacg cctgactatc aagcaacagc aaaagcagca
    15001 gcagaaaaac taggtttgga tcttaaagaa ggcgtatata tgggctattc tggaccaact
    15061 tatgaaacgc ctgcggagat ccgaatgtct cggacaatag gagccgatgc agtagggatg
    15121 tcgactgttc cagaagtcat cgtagcagct cacagcggat taaaagtgtt aggaatctct
    15181 tgtattacca acttagcagc aggtatgcaa gcgaatctga gccatgaaga agttgtagaa
    15241 acaactcaga gagtcaaaca atcttttaaa gcattggtta aagaaacatt gacattattg
    15301 taataggagg aacaataaaa tgagtgtgca tattgaagcg aaagaaggcg agatcgccga
    15361 taagatttta cttccgggag atcctttgcg agctaaatat attgcggaga cttttttgga
    15421 agatccagtt tgttacaatc aggtacgcgg tatgttaggc tttaccggca aatataaagg
    15481 agaacgtatt tctgttcaag gaacaggaat gggaatgcca tcagccacca tctatgccca
    15541 tgaattgatc cagtcgtatg gtgtaaagaa attgattcga gtaggtacat gcggtgcatt
    15601 gtcaaaagac gtccatgttc gtgatttagt actagcacaa ggtgcagcga ccagttcttc
    15661 gatgatcgaa aaaaatttcc aagcctttca ttttcctcca atcagtgatt tcaatctatt
    15721 actgaaagca tatgagatag ctaaagaaaa gggctatacg gttcatgtag gaaatgttct
    15781 ttcggaagat tctttctaca aagatgattt gacagagacg ttccaattag ctgaacttgg
    15841 cgttttagga gtggaaatgg aagcagcggc tctttattac ttaggcgcga aataccatgt
    15901 gcagacatta agtttgatga ccgttagcga ccatttgatc accggtgaag aaacaacagc
    15961 tgcagaacgt caatctacgt tcaatgaaat gatcgaagta ggattggaaa cagcgatagc
    16021 agaataagaa ggaaatgaaa aagctgccct gcattaagta ataagaacat ccaagcaaaa
    16081 atagctcctc atgtttttga cttggctgga cttattattg caaatgtaag cttttgaaca
    16141 ccgtttacca agaggttgtg aatctgccgt ttaactctga gtcacaagga acaatttcaa
    16201 aaaacagctt ttcatgtttg atgaatattg tgacttgtga ccgagaagtt ggcaggagaa
    16261 aaccgtttat acgaaaaaag ggactgtgac aaatcgtgtc acagtccctt ttttatagtg
    16321 ttttttatag gtagtatttt ttagcaactg ttaagtcatc atttaattca taaacaagtg
    16381 gttgacctgt tggaatttca agatccatga tgtcttcgtc agagatacct tcgatatgtt
    16441 tagctagggc gcgtaatgag ttaccatgtg cagctactaa aacagttttg ttgtctagta
    16501 atgctggagc aatttcatct tgccagaatg gcaaagcgcg ttctaatgtc actttcaagt
    16561 tttctccacc aggaatatcg cgtttgtcta acattgcgta acgacggtca tttgctgcag
    16621 aaccttcgtc agtttcttcc attaatggag gaagagtatc gtaagaacga cgccagatgt
    16681 gcacttgttc gtcgccgtat ttttcagctg tttctttttt gttcaaacct tgtaatttac
    16741 catagtgacg ttcgtttaag cgccatgatt tgatttgtgg tacccaaagt tgatctgaat
    16801 attctaagat caagttacat gttttgattg cacgagttaa aacagaagtg taagcaacat
    16861 caaattcgat tccagcttct ttgattttgc gtccgccttc ttttgcttct tcgattcctt
    16921 ctggtgctag atttacatca gcccaaccag taaattggtt caatgcattc cattcactaa
    16981 gaccgtgacg agaaaaaact aattttggca tgtttagctc tccttttaag ataggtttat
    17041 atttctgaaa ttttcagttc tgttttattg tacaattttt tggaagagat ttccatcgaa
    17101 aaattagata agattctatg agaaaacaag tagagaatat aaaaaaacga acagtgccca
    17161 cgcaactgtt cgtttttagg tattagatgc ataattgctc gatttctttt tcaggtaaat
    17221 tgatatattt ataacatgtt ccaactgaaa caccgcattt tgaagaaata aattggatcg
    17281 tttctttttt ttcataatag agacgtcgga tttttttgat cgtttttgca tcgatttttg
    17341 gtcttccgcc gatttttcct tttttacgag cttcatctaa tccaactaat gtacgttctt
    17401 tgatcaatgc gcactccatg tttgctaggt tttccattaa ttggaaatag atttttccca
    17461 tttcgcgcgt atcgattcct tcatcaagac taatcaagtg cagcccgcga tctttgaata
    17521 attgcgtgag ttctgttaat tgccgcgtag atttgcctaa tcgatgaagt tggcaaatga
    17581 ctagtgcatc gccttgttgc atgtcagcga tcactgactc caaatgaaca gcagtagaat
    17641 cattttgttc cacttcatat gtctcatgga aaatttcttg acaaccaaag tcagataatt
    17701 gatcaacctg agttgttaaa tcaacgtcat ttatggttgt gcgtgcataa ccaataattt
    17761 tcatggtgat tcctttctag cagtgatttt ctaactggat ataacagtaa aaatgtagca
    17821 taaagtacgt caaaaagata gttttttcgc aaattttccg ctgtcttttt ttattggatg
    17881 atcaagcagt ttttctttat ttacgacgga ttgcgtcaag aatctcaacg cctttttctg
    17941 agccgacaac tactttagaa accatgttta ggaaaagccc atgttctact accccaacaa
    18001 gttcatctaa ataggtagct aagctttctg gctgttcgat gacttctaga tgcagatcaa
    18061 tgatgtaatg accaccatct gtggttaaga cttctccgtt ttcgtctgta cgcaagacgg
    18121 gttggaatct tttttcttca aaaatatgaa gtagctgttg ggagccataa gggatcactt
    18181 caactgggag tgggaacttc ccaagtttat cgactaattt agaagaatca acgatccaaa
    18241 tcgtttcttt tgagtacgta gcgactattt tctcgaaaag caaagcagcg ccgccgcctt
    18301 tgatcccttg aaaatcagca ctgatttcat cagcaccgtc tattgtcaaa tcgactacgg
    18361 ggacttcatc aatactcttt aacggtattc ctaatttttc cgcttgttct tttgtcgcat
    18421 ttgaagtagt aactcctgtg atacgtaaac cttcttcacg catacggcga ccaatctctt
    18481 ctaccatata ataggcggtt gaaccggtcc cgagacctac gatcatcccg tcctttacga
    18541 atttcgcggc ttcaatgcca gctaattgtt ttaaattcat ctttgcgtcc accttttttt
    18601 agtttgagca tcgtgctttc ctcactattt tagcgtgatt tacggtaaaa agataccatt
    18661 cttttttatt ccaataaagc tttttcaaaa aaaattgcat ctcttgttga catgtttttt
    18721 cattcgtgat attctaacaa aggtgcttta tctacaggtt tctgtgaaga aacgtgctga
    18781 ctgtttatca aagcatattt gataaggatg cagacattgc atttattttt tatcacaaaa
    18841 aaagaaccac ctggatgtgt ggaactagac gcaaatcgtg ggaaggagga aacaaggaat
    18901 gcctacaatt aaccaattag tacgtaaacc tcgtaaatca aaggtggaaa aatctaattc
    18961 acctgctttg aacaaaggat ataacagctt taagaaaact caaactaacg taaactcacc
    19021 tcaaaaacgt ggggtagcaa ctcgtgtggg tacaatgaca cctaaaaaac cgaactcagc
    19081 tttacgtaaa tatgcacgtg ttcgtttgtc aaacttaatt gaagttactg cttatatccc
    19141 agggattggt cataacttac aagaacacag tgttgtatta ttacgcggtg gtcgtgtaaa
    19201 agacttacca ggggtacgtt accatatcgt tcgcggtgct ttagatactg ctggtgttaa
    19261 cgaccgtaaa caaagccgtt ctaaatacgg aactaaaaaa cctaaagctt aattttaatt
    19321 attaaacaaa tgaaacttgc ttctactata tagaagtaat tactatgaac aatatttcgg
    19381 aaggaggagt tacggatgcc acgtaaaggt cctgttgcaa aacgtgatgt tttaccagat
    19441 cctatttata actcaaaatt agtaactcgc ttgatcaacc gtgtaatggt tgacggaaaa
    19501 cgcgggattg cagctaacat tatctataat gcctttgact tgatcaaaga atctacaggt
    19561 aacgatccat tagaagtgtt tgaacaagca atgaaaaacg ttcaacctgt tcttgaagtt
    19621 aaagcacgtc gtgttggggg ttcaaactac caagtaccag ttgaagttcg ccctgaacgc
    19681 cgcgttactc taggtcttcg ttgggtagta aactacgctc gcttgcgcgg tgaacacaca
    19741 atggaagaac gcttagcaaa agaaatcatg gatgctgcaa acaacacagg tgcttctgtt
    19801 aaaaaacgtg aagacactca taaaatggct gatgccaacc gtgcgtttgc tcactatcgc
    19861 tggtaagatt ctctctgttt ggtcggcttt tataagtcga cctcagatag tgtccttatt
    19921 tttagcaact agatagtaaa gagaggagaa acaaggagaa tgaccagaga attttcttta
    19981 caaaacactc gtaatatcgg tatcatggcc cacatcgatg ctggtaaaac gactgctact
    20041 gaacgtatct tgtactatac tggtaaaatc cataaaattg gtgaaactca tgaaggagct
    20101 tcacaaatgg actggatgga acaagaacaa gaacgtggta ttacgatcac ttctgctgct
    20161 actacagcac aatggaaggg tcaccgaatc aacatcatcg acacaccagg acacgtagac
    20221 ttcacagttg aagttgaacg ttcattgcgt gtacttgatg gtgctgtaac tgttcttgat
    20281 gcacaatcag gtgttgaacc tcaaactgaa acagtttggc gtcaagcaac aacttatggt
    20341 gtacctcgta tcgtatttgc aaacaaaatg gacaagatcg gtgctgactt cttatattca
    20401 gtatcaactt tacatgaccg tttgcaagca aacgctcacc caatccaatt accaattggt
    20461 gcggaagacg acttcacagg aattatcgac cttgtaacaa tgaaggctga aatgtataca
    20521 aacgatcttg gaacagagat cgaagaaaca gaaattcctg aagaataccg cgaattagct
    20581 gaagaatggc gcgaaaaatt ggttgaagct gtagctgaaa ctgatgaaga acttactttg
    20641 aaatacttag aaggtgaaga aatcactgaa gctgagttaa aagaaggtat ccgccgcgca
    20701 acagttaacg tagaattcta cccagtacta tgtggttcag catttaagaa caaaggggtt
    20761 caattactat tggacgctgt tcttgattac ttgccatcac cgttagatat cccagctatc
    20821 aaagggattg accctaaaac tgatgaagaa gtagaacgtc ctgcagacga cagcgctcct
    20881 ttctcagctt tagcatttaa agttatgact gacccattcg taggtcgttt aactttcttc
    20941 cgtgtgtact ctggtgtttt acaatcaggt tcatacgtac aaaacgcaac aaaaggcaaa
    21001 cgtgaacgtg ttggtcgtat cctacagatg catgccaact ctcgttcaga aatctctgaa
    21061 gtttacgctg gagatatcgc tgctgctgtt ggtttgaaag acacaactac tggggacaca
    21121 ctatgtgatg aaaagagtct agttattctt gaatccatgg aattcccaga accagttatc
    21181 caagtcgcta ttgaacctaa atcaaaagct gaccaagata aaatgggtgt ggctttacaa
    21241 aaactatctg aagaagatcc tactttccgt gctgaaacaa acgtggaaac aggcgaaaca
    21301 atcatcgctg gtatgggtga acttcactta gatatcatcg ttgaccgtat gcgtcgtgaa
    21361 ttcaaagttg aagctaacgt aggtgcgcct caagtatctt accgtgaaac tttccgtgct
    21421 ggcactcaag ccgaaggtaa gtttgtacgt cagtctggtg gtaaaggtca atacggtcac
    21481 gtatggattg aatttacacc aaatgaagaa ggtgctggtt tcgaatttga aaatgcgatc
    21541 gtcggtgggg ttgttcctcg tgaatacatt ccagctgttg aaactggttt gaaagatgct
    21601 atggaaaatg gtgtcttagc tggctatcca ttagtagata tcaaagctaa actttacgat
    21661 ggttcatacc atgatgtcga ttcaaatgaa actgccttcc gtgttgctgc ttctatggca
    21721 ttacgtgcag cagctaagaa agctaatcca gtaatccttg aaccaatcat ggctgtagaa
    21781 gttgttatcc ctgaagatta tttaggtgat gttatgggtc acgtaactgc tcgtcgtgga
    21841 cgtgttgaag gtatggaagc tcgcgctggc ggccaacaag ttgttcgtgc gatggttcca
    21901 cttgctgaaa tgtttggtta tgctacaact cttcgttctg ctactcaagg tcgtggtaca
    21961 tttacaatga catttgacca ctacgaagat gtaccaaaat cagtacaaga agaaatcatt
    22021 aagaaaaacg gcggaaaagc tgaataattt cttgaaaccg gatagtcctg caaagagatt
    22081 atccaaagag actatatttt taatatagtt tcgtgtaaaa taattatcga tgatatgggc
    22141 atgtggtgta ccttgtgtac atcatatacc tatcagacaa ataaaaaata tattgtattt
    22201 ttaggaggac attttaaaat ggcaaaagaa aaatttgacc gttctaaacc acacgttaac
    22261 attggtacta tcggacacgt tgaccatggt aaaactacat taacagctgc aatcacaact
    22321 gtactatcta agaaaaacgg cggccaagct atggcttacg atcaaatcga tggtgctcca
    22381 gaagaacgcg aacgcggaat cacaatctca acagctcacg ttgagtatga aacagataca
    22441 cgccactacg ctcacgttga ctgcccagga cacgcggatt acgttaaaaa catgatcact
    22501 ggtgctgctc aaatggacgg agctatcttg gtagtttctg ctgctgacgg cccaatgcct
    22561 caaactcgtg aacacatcct attgtctcgt caagttggtg ttccttacat cgttgtattc
    22621 ttgaacaaag tagacatggt tgatgacgaa gaattactag aattagttga aatggaagtt
    22681 cgtgacctat taacagaata cgaattccct ggtgacgatg ttcctgtagt tgctggatca
    22741 gctttgaaag ctctagaagg cgacgcttca tacgaagaaa aaattcttga attaatggct
    22801 gcagttgacg aatacatccc aactccagaa cgtgacaacg acaaaccatt catgatgcca
    22861 gttgaagacg tgttctcaat tactggacgt ggtactgttg ctacaggtcg tgttgaacgt
    22921 ggacaagttc gcgttggtga cgaagttgaa gttgttggta ttgctgaaga aacttcaaaa
    22981 acaacagtta ctggtgttga aatgttccgt aaattgttag actacgctga agctggagac
    23041 aacattggtg ctttactacg tggtgttgca cgtgaagaca tccaacgtgg acaagtttta
    23101 gctaaaccag gtacaatcac acctcataca aaattctctg cagaagtata cgtgttgaca
    23161 aaagaagaag gtggacgtca tactccattc ttcactaact accgtccaca attctacttc
    23221 cgtacaactg acgtaacagg tgttgttgaa ttaccagaag gaactgaaat ggttatgcct
    23281 ggcgacaacg taacaatgga agttgaatta atccacccaa tcgctatcga aaacggtact
    23341 aaattctcaa tccgtgaagg cggacgtaca gttggtgccg gtgtcgttac agaaatcaaa
    23401 gcttaattca cttaagtttt aaagtaaagc aacatttatt cggacgtaat cgctcggaac
    23461 gaaggtctta ggatctttgt tccgagcttt tttatttatt gcaggatcag gaaaaaagac
    23521 aggagtatta taaatagaag aaacacgaaa taaaagagtc agtattttct aaatgaaact
    23581 ttatgaaaac aaaatgtttt ttacaagaaa aagccttgct ttgtgagttt atatatatta
    23641 taatgacaaa ggtgcgttta ctttagtgaa ttgcacagac gtaatcagaa tgatttcgtc
    23701 ggctaagaaa cgagaggttg cgacacgccc ggacgctttg ccacgaacga gcgtgacgga
    23761 aattttcgtg gagctatgtc tacttttcaa aataggcgaa ggagggaaca agatggcaaa
    23821 acaaaaaatt cgtatccgtt taaaagcgta tgaacaccgt attttagatc aatcagcgga
    23881 taaaatcgtg gaaactgcaa aaagaactgg agctagcgtg tctggtccaa ttccattacc
    23941 aactgaacgc agtctttaca caatcatccg tgcgactcat aaatacaaag attcacgcga
    24001 acaattcgaa atgcgtacac acaaacgtct tatcgacatt gtgaacccaa caccaaaaac
    24061 tgttgatgct ttgatgaagc tagacttacc atctggtgta aacatcgaaa tcaaattata
    24121 aaactaaaac aaaaataatg gaggtgtact catgaccaaa ggaatcttag ggaaaaaagt
    24181 gggaatgaca caaatcttca ctgaatctgg tgaattaatt ccagtaactg ttgtagaagc
    24241 tactccaaac gtagtactac aagtgaaaac aatggaaacg gatggctacg aagctattca
    24301 agttggttac caagatatgc gtgaagtttt atcaaacaaa cctgcgaaag gtcatgttgc
    24361 aaaagcaaac acggctccta agcgcttcat tcgtgaattc aaaaatgttg agctaggaga
    24421 atatgaagta ggaaaagaaa ttacagtaga tgttttccaa gcaggagaca ttattgatgt
    24481 aacaggtact acgaaaggta aaggattcca aggggttatc aaacgccacg gacaaagccg
    24541 cggaccaatg gctcacggtt ctcgttacca tcgtcgtcct gggtcaatgg gtccagttgc
    24601 acctaaccgt gtatttaaaa acaaacgtct tgctggccgt atgggtggca accgcgtaac
    24661 tatccaaaac ttggaagttg tacgtgtcga tgcagaaaga aatgttatct tgatcaaagg
    24721 taatgttcct ggagctaaaa aatctttaat tactatcaaa tcagctgtga aagctaaata
    24781 attgtaggaa gaaaggagga actaaggaat gccgaatgta gcattattca aacaagatgg
    24841 aagccaaaac ggtgaaatca cacttaacga agaaatcttc ggaattgaac ctaatgaaag
    24901 tgttgtttac gatgcaatcg taatgcaacg tgcgtcatta agacaaggaa cacacgcggt
    24961 taaaaaccgt agcgctgttc gcggcggcgg acgtaaacca tggcgtcaaa aaggaacagg
    25021 tcgtgcacgt caaggatcta tccgctcacc acaatggcgt ggaggtggcg tagtctttgg
    25081 accaacacca cgttcttaca gctacaaact tcctaaaaaa gttcgtcgtt tagcaatgaa
    25141 atctgtattg tcagaaaaag ttgctgaaaa caacttggta gcaatcgaag gcttgaactt
    25201 cgacgcacca aaaacaaaag aattcaaaca agttcttgct aacttatcta tcgactctaa
    25261 agtattggtt gttctagaaa aaggtaacga ctttgctgct ttatcagcac gtaacttacc
    25321 aaacgtttct gtagtaactt ctgataatgt gagtgtgcta gacgttgtat caaatacaaa
    25381 agtgttagca acacaaactg ctcttactca aattgaggag gtgcttgcat aatgaactta
    25441 ctagacgtaa tcaaacgccc agtgatcact gaaaaatcaa tgcttgccat ggatgacaag
    25501 aaatacactt ttgaagtgga cactcgcgca aacaaaactt tagtaaaaca agcggtagaa
    25561 gctgcttttg acgttaaagt caaaaacgtg aacatcgtga acgtacgtgc gaaattcaaa
    25621 cgtatgggca aatatgcagg atatacaaaa aaacgtcgca aagcgatcgt tacattgact
    25681 gaagattcaa aagaaatcca attattcgaa gctgctgaat aagcactaat agataactca
    25741 ttaaattagg agggaaaaac gtggcgatta aaaagtacaa acctacctca aatggtcgtc
    25801 gtaacatgac aggttctgat tttgctgaaa tcacaacatc aacacctgaa aaatcattgt
    25861 tacaaccatt gaaaaaccat gctggacgta acaacaacgg ccgcatcact gtacgtcatc
    25921 aaggtggcgg acacaaacgc caatatcgcg tgatcgactt caaacgtaac aaagataacg
    25981 ttgttgcaac tgttcaaacg atcgaatatg atccaaaccg ttcagctaac atcgcgttag
    26041 tacactatga agatggagtg aaagcttaca tcttagcacc taaaggacta gaagtgggta
    26101 tgaaacttgt ttcaggtcca gaagcagata tcaaagtcgg aaatgctctt ccattagaaa
    26161 acattccagt tggtactgtt atccacaaca ttgaaatgaa acctggtaaa ggtggacaat
    26221 tgatccgttc tgctggtaca agtgctcaag tacttggtaa agaaggcaaa tacgtattga
    26281 tccgcttgaa ctctggtgaa gttcgtatga tcttagctgc atgccgtgct acaatcggtt
    26341 ctgtaggtaa cgaacaacac gaattaatca acatcggtaa agctggacgc tctcgttgga
    26401 tgcgtaaacg cccaactgta cgtggtagcg taatgaaccc taatgatcac ccacacggtg
    26461 gtggtgaagg taaagctcct atcggacgta aagcaccagt ttctccttgg ggtcaaccag
    26521 ctcttggtta caaaactcgt aacaaaaaag ctaaatcaga caaacttatt gttcgtcgtc
    26581 gcaaaaataa ataattactt tgaccatgtg tcgcacattt tgagaggagg ttcaccatgg
    26641 gtcgtagttt aaagaaagga ccttttgtcg atgagcattt aatgaaaaaa gttgaagctc
    26701 aagcaggggt tgaaaagaaa aaagttatca aaacttggtc acgccgttca acagttttcc
    26761 caagttttgt tggatatacc atcgcagttt atgatggacg taaacatgta ccagtttaca
    26821 tccaagaaga catggtagga cacaaattag gtgaatttgc acctacaaga acttatcgtg
    26881 gacatgctgc tgacgataag aaaacgaaac gctaatttga ggggaggata aatccaaatg
    26941 gcagaacaaa tcacatcagc taaagcaact gcaaaaacag ttcgcacttc acctcgaaaa
    27001 gcccgtttag taattgacct tatcagaggt aaaagtgtcg cagatgcaat ttcaatcttg
    27061 aaattcacac caaacaaatc tgctggaatc attgaaaaag tattgatgtc agcagttgcg
    27121 aatgcagaaa ataactttga cttagatgtt gaaaacttgg tagtaagcga agcttttgtt
    27181 aacgaaggac ctacaatgaa acgtttccgt cctcgtgcaa aaggatcagc ttctccaatc
    27241 aacaaacgta caagtcatat cacggtagtc gtatcagaaa aataaggagg gataatcagt
    27301 gggtcaaaaa gtacatccaa ttggaatgcg tgtaggcatc atccgcgact gggatgctaa
    27361 atggtatgct gaaaaagagt atgctgagtt cttacacgaa gatttaagaa tccgtaaatt
    27421 tatttcaact aaacttgctg atgctgctgt atcaacagtt gaaatcgaac gcgcaacagg
    27481 tcgcgtaaac atttcaattc acacagctaa accaggtatg gttattggta aaggcggttc
    27541 tgaagtcgaa agcctaagaa aagaattaaa caaattaact ggtaaaagaa tccatatcaa
    27601 tatcgtggaa atcaaaaaac cagatctaga tgcaaaatta gtaggtgaag gaatcgcacg
    27661 tcaattagaa aaccgtgttg ctttccgtcg cgctcaaaaa caagccatcc aacgctcaat
    27721 gcgtgctggc gctcaaggga tcaaaactca agtatctggt cgtctaaacg gagcagatat
    27781 cgctcgttca gaaggatact cagaaggaac tgttcctctt catactttac gtgcggacat
    27841 tgattacgca tgggaagaag cagatacaac atacggaaaa ctaggagtta aagtgtggat
    27901 ctatcgtgga gaaattcttc caactaaaaa aaacactgag aaaggaggga aataatcatg
    27961 ttagtaccta aacgtgtaaa acaccgtcgt gaattccgcg gaaaaatgcg cggggaagct
    28021 aaaggcggaa aagaagtagc attcggtgaa tacggtttgc aagctgttga ttcacattgg
    28081 atcacaaacc gccaaatcga agctgctcgt atcgcaatga ctcgttacat gaaacgtggt
    28141 gggaaagtat ggattaaaat tttccctcac aaatcttata ctgccaaagc aattggggta
    28201 cgtatgggtt ctggtaaagg ggcacctgaa ggatgggttg caccagtaaa acgtggtaaa
    28261 atcatgtttg aaatcgcagg cgttcctgaa gaagtagctc gtgaagcgtt acgtctagct
    28321 tctcacaaat taccaatgaa aactaagatc gtaaaacgtg aggaaatggg tggtgaatcg
    28381 aatgaaggtt aaagaaatca gagaattaac cactgccgaa atgcttgatc aagaaaaaca
    28441 attaaaagaa gaattgttta atcttagatt ccaattagca acaggtcaat tagaaaacac
    28501 tgcacgtatt aaagaagtac gtaaatcgat tgcacgcatc aaaactgtgt tgcgtgaaca
    28561 agctaagtaa ttatggaagg aggccattaa gcgtatgact gaaggaagaa atcaacgcaa
    28621 agtttatcaa ggtcgcgtgg tttcagacaa aatggataaa acaattaccg ttgctgtcga
    28681 aacaaagaaa aaccacccta tctatggcaa acgcatgaac tattctaaga aatacaaagt
    28741 gcatgatgaa aacaacacag caaaagttgg cgacatcgtg agaattatgg aaactcgtcc
    28801 attatcagct acaaaacgtt tccgtttgtt ggaagtagtc gaagaagcag ttattatcta
    28861 ataacacgag attttcctat atagattgaa tgcaaaatct gaaaggagga tacacaacgt
    28921 gatccaagca gaaagtcgtt taaaagtcgc tgataactca ggcgcacgtg aaattttgac
    28981 gatcaaagtc cttggtggat ctggtcgtaa aactgctaac atcggtgatg tgatcgttgc
    29041 tacggttaaa caagcaacgc caggtggggt tgtcaaaaaa ggtgacgtcg ttaaagccgt
    29101 tatcgttcga acaaaatcag gagctcgtcg tacagacggt tcacatatta aatttgatga
    29161 aaatgctgca gtgattattc gtgatgataa aagccctcgt ggaacacgta tctttgggcc
    29221 agttgcacgt gaactacgtg aaaacaactt catgaagatc gtttcactag caccagaagt
    29281 tttataatct tgagacactt atcaaaggag gtgcgaaaca gttatgtttg ttaaaaaagg
    29341 cgataaagtt aaagttatca ctggaaaaga caaaaacaaa gaaggcgttg tactagcagc
    29401 gttcccgaaa aaagataaag ttattgtcga aggtgtaaat gtcgttaaaa aacaccaaaa
    29461 accttctcaa gctgctcctc aaggtggaat tgttgagatg gaagcgccaa ttcatgtttc
    29521 taacgtgatg gtggttgact ctactggtgt agctggccga gttggctaca aagaagtaga
    29581 cggcaaaaaa gtccgtgttt ctaaaaaaac cggtgaagtt ttagataaat aaaagtttaa
    29641 ggaaggaggg tcttactgaa tgaaccgcct aaaagaaaaa tatcttaaag aagtaactcc
    29701 atcattgatg gaaaaattta actatagctc tgttatgcaa acacctaaag ttgaaaaaat
    29761 cgtcatcaac atgggtgttg gtgatgcggt atcaaacgct aaaaacttag ataaagcagt
    29821 tgaagaatta actttgatca ctggtcaaaa accaatgatt acaaaagcta aaaaatcaat
    29881 cgctggattc cgtttacgtg aaggaatgcc aattggtgca aaagttacat tgcgcggaga
    29941 aagaatgtac gaattcttag acaaattggt ttctgtttca ctaccacgtg tacgtgactt
    30001 ccatggtgta agtaaaaaag cctttgacgg tcgtggaaac tatactttag gtattaaaga
    30061 acaattgatc ttcccagaag tagattatga tttagtagat aaagtacgtg gtatggacat
    30121 cgttattgta acaacagcaa actctgatga agaatctcgt gagttgttga cacaattagg
    30181 catgccattc caaaaataat taaaggaggc gaactacgtg gctaaaaaat caatgattgc
    30241 taaaaacaaa cgtcctgcaa aattttcaac acaagcttat actcgttgcg aacgttgcgg
    30301 acgtccacat tcagtttatc gtaagttcca tctttgccgt atttgcttcc gcgaacttgc
    30361 ctataaaggt caaattcccg gcgtgaagaa agctagctgg taaaaagtaa attcgcataa
    30421 gaaggaggta aaagatcaat ggtcatgaca gatccaattg cagattttct aactcgtatc
    30481 cgtaacgcaa acatggttaa acatgagtct ttagaagtac ctgcatcaaa aattaaacgt
    30541 gacatcgctg aaatcttgaa acgtgaaggt ttcgttcgtg atgttgaata catcgaagat
    30601 gacaaacaag gcgtgatccg tgttttccta aaatttggta aaaacggcga acgtgttatc
    30661 acaaacttaa aacgtatctc taaaccaggt ttgcgtgctt atgtcaaagc tgacgaagta
    30721 ccaaaagtat tgaacggttt aggtatcgca atcatttcga cttctgaagg tgttgtcact
    30781 gataaagaag ctcgtgaaaa aaatatcggc ggcgaagtta tcgcttacgt atggtaatca
    30841 agtagaatct acaaggaggt gtctttaagt gagccgtatc ggaaataaag tcgttgttat
    30901 ccctgaagga gtaacagtta ctcaagacgg aaacaacgtt acagttaaag gaccaaaagg
    30961 tgaattgact cgtacttttt cagcagatat caaaatgaat atcgaaggaa acgaagtaac
    31021 tttcactcgt ccaaacgaca gtaaagaaat gaaaacaatt catggaacaa ctcgtgcgaa
    31081 cttcaacaac atggttgtag gtgtcagcga aggtttccaa aaagcgttag aactaatcgg
    31141 ggttgggtac cgtgcacaat tacaaggatc aaaacttgta ttgaacgttg gttactcaca
    31201 tccagtagaa atcacaccac cagctggagt aactgttgaa gtaccagcta acacacaagt
    31261 aattgttaaa ggtgcgaaca aagaagaagt tggcgaatta gctgcaaaca ttcgtggaac
    31321 tcgtcctcca gaaccttata aaggtaaagg tattcgttat gttggcgaat atgtacgccg
    31381 taaagaaggt aaaactggta aataatcgtt tagaaaaatt gcatagaagc cagcagcagg
    31441 aaaaaggcat ttggctgaag tttaccttca aaaaaataca tttttacttg tgccgcagct
    31501 tctatttttc gaaacgtatt tactgcttga aatagtagta caataaaaat aaagaggtga
    31561 caattgtgat tacaaaacca gataagaaca aaacacgtca aaagagacat cgtcgtgtac
    31621 gtaacaatat ctctggtact gctgagcgcc cacgcttgaa cgtttttcgt tctaacaaaa
    31681 acatctacgc tcaaattatt gatgacgtag cgggtgtaac gctagcaagt gcctctacct
    31741 tggataaaga aatttcaggt ggaacaaaaa cagaagcagc acaagctgtc ggtaaactag
    31801 ttgctgaacg tgcagctgaa aaaggcatta aagtagtagt ctttgaccgt ggtggatacc
    31861 tttaccatgg ccgtgtgcaa gctttagctg aagctgctcg cgaaaatgga ctagaatttt
    31921 agaagaagga ggaataccat tcatggttta tatcgatcca aaacatttgg aattagaaga
    31981 ccgcgttgtt gcaattaacc gtgtaacaaa agttgttaaa ggtggacgtc gtctacgttt
    32041 tgctgcttta gttgttgtcg gtgacaaaaa cggacacgta ggatttggta caggtaaagc
    32101 tcaagaagtt cctgaagcaa tccgtaaagc aattgaagat gcgaagaaaa acttagttga
    32161 agtacctatg gttggttcaa caatcccaca cgaagtaatc ggtgctttta gtggtggtag
    32221 aatcctaatg aaacctgctg tagaaggttc tggggttgct gctggtggac cagttcgtgc
    32281 cgtattagaa ttagctgggg tagcagatat cacttctaaa tctttaggct caaatacacc
    32341 aatcaacgtt gttcgcgcaa cagttgaagg tttgaaacaa ttgaaacgtg cagaagaagt
    32401 tgcagaactt cgcggcaaat cagttgaaga attaatcgga taaggaggac aacaataatg
    32461 gctgaattaa aagttacttt aaaacgcagt attatcggac gtcctcaaaa ccaacgcgat
    32521 acagttaaag cgttaggtct tacaaagatt aacagttcag tggttaagcc ttctaatgaa
    32581 gcaattaaag gcatgatcaa tactgtttct cacctagtgg acgttgaaga aatttaattt
    32641 agaaaaatca taactagaac attaaggagg tgccaaacct atgaaacttc atgaattaaa
    32701 acctgctgaa ggttcacgcc aagtacgtaa ccgtgttggt cgtggtactt catctggtaa
    32761 cggaaaaact gccggacgcg gacaaaaagg acaaaaagct cgttcaggcg gcggtgtgcg
    32821 tttaggattt gaagggggac aaacaccatt gttccgtcgt ttaccaaaac gaggattcac
    32881 aaacgtgaac cgtaaagact atgcagtcat taatcttgat gtattgaacc gctttgaaga
    32941 cggagctgaa gtaactcctg ttgctttagt tgaagctgga atcgtgaaaa acgaaaaagc
    33001 tggaatcaaa gtattagcta atggggaact aaacaagaaa ttaactgtga aagcagcaaa
    33061 attctctaaa gcagctcaag aagcaattga agcggctggt ggatcaatcg aggtgatcta
    33121 atgtttagac tattaaaaga tgcttttaaa gtaaaagaca ttagatcgaa aatcctattt
    33181 actgtatttg tgttgtttgt atttcgccta ggtgcacata ttactgtacc aggcgtaaat
    33241 gctgcttcat tgcaaaacct ttcttctttg ccatttttca acatgttgaa cttggtaagt
    33301 ggtagtgcga tgcaaaactt ttccattttc tcaatggggg tttcaccata cattactgct
    33361 tctatcgtca ttcagcttct tcaaatggac atcgtgcctc gatttgttga atggtcgaaa
    33421 caaggggaag ttggtcgtaa aaaactgaat caagcgactc gatatctgac attagtattg
    33481 gcatttatcc aatctgtcgg tatcactgct ggattcaatc aatacgctca attaggtttc
    33541 gtgaataatc ctaacgtagc aacttacgta acgatcggtt tgattctaac agccggtacg
    33601 atgtttgtta cttggctagg agaacagatc acagaaaaag gtatcggaaa tggcgtatca
    33661 atgatcattt ttgcaggtat catttctaga ttgcctgaaa gtatcagaga aatttatgaa
    33721 gattacttta taaatattga tccttcagac atatggaaat ctgctatttt tgtggccatt
    33781 ttactagtag cggtcttgtt gatcgtgaca ttcgtaacat ttttccaaca agctgaacgc
    33841 aagatcccga tccaatatac gaaacgcgta gctggcgcgc ctacaagcag ctatctgccg
    33901 ttgaaagtaa atgcagctgg agttattcca gttatctttg ctagttcgtt tattgctacg
    33961 ccaaatgctg ttttacaagc attgagcggt agattcggtt ctgaaaactg gtacaaagtg
    34021 atgatggaga tctttaacta taatacagca cctggtgcta cgatttatac gattctgatc
    34081 gttgccttca ctttcttcta cgcctttgtt caagtaaacc ctgagaaatt agcggaaaac
    34141 ttgcaaaagc aaggaagtta tataccaagc gtacgccctg gtaaggggac tgaagagtat
    34201 gtctcaagat tactgatgcg tttaagtaca gttggtgcag tcttccttgg tttagtggca
    34261 ttgcttccaa tcattgctca aatggtttgg gatcttccac aatcaatcgg attaggcggt
    34321 acaagcttac tgatcgttat cggtgtagct ttagaaactg cgaaacagtt agaaggctta
    34381 atgctgaaac gtaaatatgt tggattcatt gagtaagaaa gcgtgttgag gtttcccctc
    34441 aacacacgtt ctcacacatt tattaggagg gaaaacaatg aacctcattt taatggggct
    34501 gccaggcgca ggtaaaggaa cacaagctga aaaaatcatt gatgcttatg gtattccgca
    34561 catctcaaca ggagatatgt tccgcgcagc gatgcaaaac gaaacagctc ttggcctgga
    34621 agcaaagtct tatatggata aaggtgcatt agttcctgat gaagtaacaa atggaatcgt
    34681 gaaagagcga ctagctgaac cagatacaga gaaaggcttc ttattagatg gctttccacg
    34741 tacgctagat caagcaaaag ctctggacgc aatgctaaaa gatttgaata aaaaaattga
    34801 tgctgtcatc gatatccatg ttggtgaaga aatcttggtc gaacgtctag ccggccgatt
    34861 tatctgcagc aattgtggag caacttacca taaaattttc aaccctacaa aagttgaaga
    34921 cacatgtgat cgttgcggtg ggcatgaatt ctatcaaaga gaagatgata aacctgagac
    34981 ggttaaaaat cgtctggcta tcaacatcaa aaatagtgaa ccaatcttgg cttattataa
    35041 agaacaaggt ttgctaaaca ctattgatgg tgctcgtgaa atcgatgctg tatttgcaga
    35101 tgtacaaaaa atcattgaaa aatagcacga ttgcccgtag ccgacttgca ttatgcagtt
    35161 actcttgtca agttcaataa attatgatag aatatgtcag tccgacttct agagaatcct
    35221 ctagatgatc cattttgaga attttgaggt gggaactgca gtttccgcca ttttatcgtg
    35281 tgaaaatgcg aaacaagtac aaggaggtac tgtgcgtggc aaaagaagat atgattgaag
    35341 tcgaaggtac agtcgtcgaa actttgccga atgcaatgtt taaagttgaa ctagaaaacg
    35401 gacaccaagt tcttgctact gtttcaggta aaattcgtat gcattacatc cgtatcttac
    35461 ctggtgacaa agtaacggtt gaattgtctc catacgatct taaccgtggc cgtattactt
    35521 atcgctttaa ataattgtac tccgtaaaac tacaggaggt attatcatga aagtaagacc
    35581 atcagtaaaa ccaatgtgcg aacattgtaa agtaattcgt cgtaaaggac gcgttatggt
    35641 gatttgccca gcaaatccaa aacataaaca acgtcaagga taattggagg tgctataaat
    35701 atggctcgta ttgcaggagt agatattcct cgtgataaac gtgtagtagt ttcacttact
    35761 tatatctatg gtattggtaa cactactgct aaaaagatct tagctgatgc tggtgtatct
    35821 gaagatgttc gcgttcgtga attaacgaat gaacaaacag atgctatccg tgcagaagta
    35881 gataaattaa aagttgaagg ggatcttcgt cgtgaagtga acttgaacat caaacgtttg
    35941 atggaaatcg gttcttaccg tggaatccgt catcgccgtg gattgcctgt tcgtggacaa
    36001 aacacgaaaa acaatgcacg tactcgtaaa ggcccagctc gcgctatcgc tggcaagaaa
    36061 aaataattct taagtgaagg aggttaaaac ttcatggcag caaaaaaagt gaatcgtaaa
    36121 cgccgtgtga agaagaatat agaagcaggt attgcacata tccattctac attcaataac
    36181 actatcatca tgatcactga tactcacgga aatgcattag cgtggtcatc agctggttca
    36241 ttaggtttca aaggaagcaa aaaatcaact ccttttgccg ctcaaatggc agcagaaact
    36301 gctacaaaag cagcaatgga acacggatta aagactgttg aagtaacagt taaaggacct
    36361 ggttctggac gtgaagcagc aattcgttca ttacaagcta caggtttaga agtgactgca
    36421 attcgtgacg tgactccagt tcctcataat ggtgcccgcc ctccaaaacg ccgtcgtgtt
    36481 taatgagtgc cgctcatttt gagttttatc agtaacgtca ttgaacaaga cacttttcgt
    36541 tttgaaaggg gtaaagataa gaatgattga gttcgaaaaa ccaagaatcg caaaaattga
    36601 tgaagaaaaa gattatggca agttcatcgt tgaacctctt gaaagagggt atgggactac
    36661 tttagggaat tccctacgtc gtattttatt atcttctttg ccaggtgctg ccatcactaa
    36721 tattcaaatc gatggtgtac tgcatgaatt ctctaccatc aaaggtgtta gagaagacgt
    36781 cactcaaatc atcttgaaca ttaaaggctt agcgctgaaa atgtatggcc aagaagaaaa
    36841 aacccttgaa atcgatatta ccggacctgc tacagtaact gctggcgata ttatcgttga
    36901 tagtgatgta gaaattctta acaaagatat gtacatctgt agtgtctctg aaggagctac
    36961 gttccatgct cgcttaacag tgaaacctgg acgtggttat gttcaagcag atgaaaataa
    37021 aaaagaagat atgccaattg gcgttcttcc agtcgattca atctacacac ctgttcgtcg
    37081 tgtgaactat caagttgaga acacacgtgt tggacaccgt gaagattttg ataaattgac
    37141 gatggagatt tggacagatg gttcaattga accgttggaa gcaatgagtt tgtctgcgaa
    37201 aatcatgact gaacatttgg atatttttgt gaacttaact gatgaagcaa aaaatgctga
    37261 aatcatggtc gaaaaagaag aaacacaaaa agaaaaaatg ttggaaatga cgatcgaaga
    37321 gttagacttg tctgttcgct cttacaattg tttgaaacgt gcaggaatca atactgtaca
    37381 agaacttaca aataaatctg agcctgaaat gatcaaagtc cgtaacttag gacgtaaatc
    37441 attagaagaa gttaaagcaa aattgcacga cttaggttta ggtttacgta aagaagatta
    37501 aaacctttta cgaaggaggg aaaccaacgt gagttatcgt aaactaggac gcacatctag
    37561 ccaacgtaaa gcgatgttgc gtgatttaac aactgattta ttaatcaacg aacgtattgt
    37621 gactactgaa gctcgtgcga aagaagttcg ctcaactgca gaaaaaatga tcactttagg
    37681 taaacgcgga gatttgcatg cacgtcgtca agctgcggct ttcgtacgta atgaagttgc
    37741 cagcgtgcgt gaagaaaatg aggacatcgt cattgaatca gctttacaaa aactgttcaa
    37801 cgatattgca cctcgttacg cagaacgtca aggcggatac acacgcattt tgaagacaga
    37861 accaagacgc ggtgatggtg caccaatggt tgtcttagaa cttgtctaaa atcacactaa
    37921 taacatcact ttatagatga ttcttttaga gtgttatgat gttggaagat atttttccga
    37981 gtctagctca gcgctgctcc tcaagcgaat atcgcttggg gagtaagcac tcatcataaa
    38041 gtgttttttt ataccttcaa ttacctgtca tgacagatat gtttgaaggc ataaaaaaac
    38101 attttaagcg agaaataatt gaaaattttg tctttttttg atagtgttag taggaacttt
    38161 aggatgaaga aggagtaatc atggagccaa ttattgaatt agaaaagata aattataaat
    38221 accagccaga tgatcttcgc cccgcattga aagatgtctc ctttacaatt gataaaggtg
    38281 agtggatcgc tattattgga cacaatggtt caggaaaatc gacacttgcc aaaacgatca
    38341 atggattgct tctgccagaa tcagggatcg tgaaagtggg aaatcaaata ttggatgaag
    38401 aaaatatttg gacgatccga cagatggttg gaatggtttt tcaaaatcca gacaatcagt
    38461 ttgttggttc gacagtagaa gatgacgttg cttttggtct tgaaaatcaa gggattccca
    38521 gagaagaaat gctagtgcgt gtgaaagatg cacttgagaa agttagaatg gctgagtttg
    38581 cttcacgcga gcctgctcgt ttatcaggag ggcaaaaaca acgtgttgcc attgccggcg
    38641 tggttgcctt acgacctgat atcattattt tagatgaagc aaccagtatg ctagatccag
    38701 aaggtcgtga agaagtgatc tctacgatca agaagatcaa agaagaaagt caattgaccg
    38761 ttatatctat cactcatgat attgatgaag cagctaatgc caatcggata ctagtgatga
    38821 gacaaggcga acttgtccgt gaaggtacac caaaagaaat tttttctgct ggtcctgaac
    38881 tgatcgactt aggtctagat ctaccatttc ctgaaaaact gaagagcgca ctgaaagaac
    38941 gtggggttga tgtgcccagt gaatatatga cagaagaaag gatggtggat tggttatgga
    39001 catccgtttt gaacaagtag acttcactta tcagccgaat acaccatttg aacaaagagc
    39061 cttatttgac atcaatatga cgatcaaaga aaacagttat actgcattag tagggcatac
    39121 cggaagcggg aaatcaacct tacttcagca tttgaatgcg cttgttaagc caacaagcgg
    39181 tacagtccat attggagaac gagatattca gccggatacg gataataaga atttaaagcc
    39241 catccgaaaa aaagttggca tcgtctttca gtttccagaa gcacagctat tcgaagaaac
    39301 ggtagcaaaa gacattgctt tcggtcctaa aaactttggt gtcagcgaag aagaagcact
    39361 agtcctagca aaagaaacat tagaacaagt tgggctggat gaaagctatt tggaacgttc
    39421 gccatttgaa ctttcaggag ggcaaatgcg tagagtagca atcgctggtg tgcttgccat
    39481 gaggccggaa gtacttgtat tggatgaacc aacggcagga cttgatcccc agggcagaaa
    39541 agaaatgatg gagatgtttt ggcgtcttca taaggaacag cagatcacta ttgtattagt
    39601 tacgcattta atggatgatg tagccaattt tgcagattat gtctatgtgt tggaaaaggg
    39661 acgaatcgtt aatagcgggg agccgcaaga ggtctttcaa aacattgaat ggctaaaaga
    39721 aaaacaacta ggcgtaccta ctgccaccga gtttgcagaa gaattaatgg ctaaagggat
    39781 gaactttgct accttgcctt tgacagctga agaattagca gacgctatcg tccattatgc
    39841 tggaggaaat gcccatgatg aataaattga tatttggtcg ttatatgcct ggagattcct
    39901 tgatccatcg attggatccc cgagctaaat tattagccag tttttatttt atcggaatca
    39961 tttttttagc aaataattgg gaaacgtacc ttttgttggc atttttcaca ttactttctg
    40021 tcttcttgtc aaaagtagat ttgaaatttt ttatccgagg cattcgccca ttgatctggc
    40081 taatcctctt cactgtagct ttgcaggtct tgtttacaag tggcggagaa acttattgga
    40141 gttggggtgt tttccatatc actgaatttg gtgtgatgaa cggcctcttt attttctgtc
    40201 gattcgtgtt gatcattttt atgtcgactc tactcacgct tactacaccg cctttggaat
    40261 tatcggatgc gattgagtac ttgcttcgtc cattgaaagc cgtacggttc ccagtccacg
    40321 aaatttcatt gatgctgtcg attgctcttc gttttgtgcc tacattgatg gacgaaacag
    40381 aaaagatcat gaacgctcag cgcgctcgcg gtgttgattt tggtgaagga aacctagttc
    40441 agaaaatgaa agcagtcatt cctttgctga ttcctttatt tgtcagcagc ttcaaccgtg
    40501 cggaagatct tgcgactgct atggaagcaa gaggctacaa aggtggggaa ggaagaacga
    40561 aatatcgtgt attgcattgg cataatagag acacgatggt tatcattgct tttgtattat
    40621 taactgtgct actgatcttt ttaagaggat aaacgttcag tgggccgcta tctattcttt
    40681 tcttgctgat ctgtttgcaa gaaaggatgc aagcagtgta tgacaagaaa tattagggtc
    40741 tgtggcagac taaggtcaca ggcctctttc ttttatctga aaagaaagaa cgaaaaaatt
    40801 ttatagaaaa atcattattc cagataacct tttaggaaca aaggagagaa aaacatggta
    40861 cgttacaaag caatcattgc gtacgacgga acaaatttta atggattcca aaaacagcca
    40921 aatgggcgta ctgttcaaga agaagtagaa aaaacattac aaaaaatggc aaatggaaaa
    40981 gaaatcacgg tctttggttc gggaaggaca gatgccggag tacatgcgat cgggcaagta
    41041 atccattttg attatccaga ggagcgtccg ttagaacgaa tgcgtttcgc gctagatacc
    41101 cagtcaccag aggatattgc tgtaagacaa gtagaaattg tttcagaaga cttccacgca
    41161 agatatttgg tcaaagaaaa gacgtatcaa tttcgagtgg atatagggaa accacgcagt
    41221 ccttttcgac gccattatgc aagttatttt ccttacccac ttgatttgga aaaaattcag
    41281 cgggcgcttc ctgatttatt agggactcac gatttcacct ctttttgtgc atcaggaagc
    41341 tcgatagagg ataaaatacg tacgatctac gaggctaaga tggaggtaaa tgaaacaaaa
    41401 gacgaattgt tgtttacatt taggggaaat ggcttcttgt ataagatgat acggatctta
    41461 gtaggaacgc ttttgaaaat tggcaatggc cgattgcctg aagatagtat cccagatatt
    41521 attgcgaaaa aagatcgtaa tgcagcagga cctacagcac accctgaagg actttatttg
    41581 tatgaagtga aatatgaata agaggttatg atagaagtgg tcagcttcaa gaaataagaa
    41641 ggtatttacg aaaattactt ctcaaaattt tgcaaaattt cggcttattt tcgaagaagc
    41701 tacttctgga acaccgttta aggaagaggt cgtgaaatca gcgttttgac ctgagtatta
    41761 gagaaacaaa cgggaaaata gctcctcata ttttgccgtt tgttgagcta ataccgaagg
    41821 tcagctgatt gaacaccgtt taagaaagag gtcgtgagcc tgtcatttaa ctctgagtca
    41881 caaagagcaa tttcaaaaaa cagcttctca tctttgatga atatcgtgac ttgtggacga
    41941 ggcgttgata agtgaatcat ctttttgtta cctatttttt ctgtaaccaa aaaacgatgg
    42001 aacgttgttt tgattgccct tggtgcggac agtctgaaca ggttccatcg tttctttatt
    42061 ttctacgtct tcttctgtgt gtgtttcgtg gttgttcttc tattgctgga tcaatcgtgc
    42121 cataaaaggg gcgattgcgg tcaaggtacc agacataaag aataaacaat gcaatacata
    42181 ggacaaaaag gattgcacct atcgtaaatc cttcaggcaa gtaactgaaa gtgacagtat
    42241 gtttacctgc tggaactttt atcatcaaga atgcgtcttt gaaattgctg accttgactg
    42301 gtttcccatc aattttagct ttccatcctt tgtcataagg aatagttgtc accaacatgg
    42361 aatcttcagc ggtatttaca gtcccagaag cttttctgcc agtggcttcc atttcgacac
    42421 ctttttcttt gatctgattc attgcggctt gataggctac aacatctaga ccaacgactt
    42481 taggctccat gaaacttatt tctttcgtcc cataaaaact tgcagtaaat gtcactgttg
    42541 tctcttgagg ataatagcca agatcatagt attgaccaga aatgttgatt tgcgtttttc
    42601 gcgatgtacc attgacagtg atcgtcgcac tagaactttc taattgcgca aaattggttg
    42661 ggaacaaact taagtaagct tgtgtatttg caggcacatg gacttcccaa gtgagctctt
    42721 ttggctggtt atctgctgtt tctgagtaag tgacgccagt cgttgtcttt gtgatcgtcg
    42781 tattcaactg attcttcaaa gtaggtgtat agaatgtaaa atactgttca tttagaccag
    42841 acaatgtatt gatcaagttt gtctgactgg tcaaattatc tgtcactggc tgctcaacat
    42901 cattaacgga caacggtgca gtaaatgcta gtggcaatgc attgacattc tgatacagtg
    42961 tatagtcgcc gctttgacca gcttggcgga agccatattt actgaacgtg ctttcgtttt
    43021 tagaaatgtt atatttgatt cctgtaaacc catccatcag caaagtatta ttgttataac
    43081 ggatattcaa atttgtccct cttgaacgga aacctaattt atccaggtaa gaggaagaat
    43141 tacgatttct tatagaagaa aacaaactga taccactgta accatagttg atcgaatcgt
    43201 ttgcagatac tggatccaga ttttccaagc ggtaaaacga gtcggaatct ttttttgttt
    43261 catttacaag agctttcaag tcaggataag gatcagtata taaactgcgg gaagcataat
    43321 tccaatcatc aagaatgcca tggatcatac tgttcgtatt gaccagtgct tcagcagaca
    43381 tgatcaataa taacaatata ctcagatgat tgaatgaaag ttttttatgc tgataaaatc
    43441 ccacagcaaa agcataaatc cctaagaaaa ccactgtcaa tacaaaggaa gtcatcgtca
    43501 cataggtgta gcttccttca ggtttgatgc cccatgccac cgcaaagaga gcagctacga
    43561 ccaaaatgat tcctaacaac agcccacgat cgtcttgttc tatcttttcc catccttttc
    43621 ctgcaagcag gagcactaag aaagagaaca gataggcata acggaataaa aacatattcg
    43681 gtgcatgcat accatgccag aacaaattca atggaacaag ataaaaactt gcgataagta
    43741 tggcgaaaag actgccgtac aaaaatttat ttttcaacgg tactttccga ctggcaaaat
    43801 aaaacagaca gaggactaat ggtagcagac ctgcgtaaac gaacggaatc gatccgtatt
    43861 ttgtcgtgtc ataaacacca atcatatttt tcatgattag atccagataa gaagtcgctt
    43921 ctgttttcaa cgtagtgatc tgactcagtg cctcgccatt tgaccgaaga tccagcactg
    43981 ctggcaatac aatgatcatt gaagcgccgc ctgccagtaa agaagtgata ccataaggaa
    44041 caatcgtttt tttatattct gaccagtttg tcaatagacg ggcgataaaa tataagaaag
    44101 aaaatacgcc aatcataaaa cccatataaa aactggataa aaacaagagt aaataactga
    44161 taaataaaag tttcggccgt ttaagatcca taacacgatg aatccccaaa atcactaatg
    44221 gaagataaaa aaatgcatcc agccacatga tgatctccga atgtgcagtg ataaatgaga
    44281 tcaatgcgta actgacgcta agtgcaacat gcccccagcg aggaatcttg aacgtacgtg
    44341 caagaaacca aaaagccaat ccggcacagc cgaccttaag caaagtcaga acatacagtg
    44401 catctggcat catctggttt ggaaaaaaga acaccagtgg cgtgaagatc ccgcctagat
    44461 agtaagaaat taaagataaa taattcaatc ctagagaagc attccatgta taaaaaatac
    44521 tttgctttcc aagaagaaca tttcggaagc tggcatgaaa attagaaaac tgtgaaaagg
    44581 catcacttgc caaaatactt ctgctgcttc caggatagat tccgatactt agataaacaa
    44641 gtgctaaaat caaaaacgga atcaggaaac tagctgctgc gtagcgtccg ttttctctaa
    44701 agaatgactt taaatttttt ttcatattta ttttccacca
//
LOCUS       DACESX010000006        42709 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.006, whole genome shotgun sequence.
ACCESSION   DACESX010000006 DACESX010000000
VERSION     DACESX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.006"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            265..954
                     /locus_tag="IXF88_000297"
     CDS             265..954
                     /locus_tag="IXF88_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1507058.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            941..2149
                     /locus_tag="IXF88_000298"
     CDS             941..2149
                     /locus_tag="IXF88_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1507059.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2443..3714
                     /gene="serS"
                     /locus_tag="IXF88_000299"
     CDS             2443..3714
                     /gene="serS"
                     /locus_tag="IXF88_000299"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1507060.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4263..5747)
                     /gene="guaB"
                     /locus_tag="IXF88_000300"
     CDS             complement(4263..5747)
                     /gene="guaB"
                     /locus_tag="IXF88_000300"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1507061.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6252..6947)
                     /locus_tag="IXF88_000301"
     CDS             complement(6252..6947)
                     /locus_tag="IXF88_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1507062.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7037..8137)
                     /gene="ychF"
                     /locus_tag="IXF88_000302"
     CDS             complement(7037..8137)
                     /gene="ychF"
                     /locus_tag="IXF88_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1507063.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8146..8337)
                     /locus_tag="IXF88_000303"
     CDS             complement(8146..8337)
                     /locus_tag="IXF88_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1507064.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8358..9248)
                     /locus_tag="IXF88_000304"
     CDS             complement(8358..9248)
                     /locus_tag="IXF88_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1507065.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9235..10002)
                     /locus_tag="IXF88_000305"
     CDS             complement(9235..10002)
                     /locus_tag="IXF88_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1507066.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10014..10727)
                     /gene="rsmG"
                     /locus_tag="IXF88_000306"
     CDS             complement(10014..10727)
                     /gene="rsmG"
                     /locus_tag="IXF88_000306"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1507067.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10959..12860)
                     /gene="mnmG"
                     /locus_tag="IXF88_000307"
     CDS             complement(10959..12860)
                     /gene="mnmG"
                     /locus_tag="IXF88_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1507068.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12877..14274)
                     /gene="mnmE"
                     /locus_tag="IXF88_000308"
     CDS             complement(12877..14274)
                     /gene="mnmE"
                     /locus_tag="IXF88_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1507069.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14618..14956
                     /locus_tag="IXF88_000309"
     CDS             14618..14956
                     /locus_tag="IXF88_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507070.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15124..15882)
                     /locus_tag="IXF88_000310"
     CDS             complement(15124..15882)
                     /locus_tag="IXF88_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1507071.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15900..16718)
                     /locus_tag="IXF88_000311"
     CDS             complement(15900..16718)
                     /locus_tag="IXF88_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1507072.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16715..17071)
                     /gene="rnpA"
                     /locus_tag="IXF88_000312"
     CDS             complement(16715..17071)
                     /gene="rnpA"
                     /locus_tag="IXF88_000312"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1507073.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17189..17323)
                     /gene="rpmH"
                     /locus_tag="IXF88_000313"
     CDS             complement(17189..17323)
                     /gene="rpmH"
                     /locus_tag="IXF88_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1507074.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17846..19180
                     /gene="dnaA"
                     /locus_tag="IXF88_000314"
     CDS             17846..19180
                     /gene="dnaA"
                     /locus_tag="IXF88_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1507075.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19378..20508
                     /gene="dnaN"
                     /locus_tag="IXF88_000315"
     CDS             19378..20508
                     /gene="dnaN"
                     /locus_tag="IXF88_000315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1507076.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20737..20979
                     /gene="yaaA"
                     /locus_tag="IXF88_000316"
     CDS             20737..20979
                     /gene="yaaA"
                     /locus_tag="IXF88_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1507077.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            20966..22090
                     /gene="recF"
                     /locus_tag="IXF88_000317"
     CDS             20966..22090
                     /gene="recF"
                     /locus_tag="IXF88_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1507078.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22087..24033
                     /gene="gyrB"
                     /locus_tag="IXF88_000318"
     CDS             22087..24033
                     /gene="gyrB"
                     /locus_tag="IXF88_000318"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1507079.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24053..26524
                     /gene="gyrA"
                     /locus_tag="IXF88_000319"
     CDS             24053..26524
                     /gene="gyrA"
                     /locus_tag="IXF88_000319"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1507080.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26760..27059
                     /locus_tag="IXF88_000320"
     CDS             26760..27059
                     /locus_tag="IXF88_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1507081.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27109..27621
                     /gene="ssb"
                     /locus_tag="IXF88_000321"
     CDS             27109..27621
                     /gene="ssb"
                     /locus_tag="IXF88_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1507082.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27647..27883
                     /gene="rpsR"
                     /locus_tag="IXF88_000322"
     CDS             27647..27883
                     /gene="rpsR"
                     /locus_tag="IXF88_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1507083.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28026..29999
                     /locus_tag="IXF88_000323"
     CDS             28026..29999
                     /locus_tag="IXF88_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1507084.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30005..30457
                     /gene="rplI"
                     /locus_tag="IXF88_000324"
     CDS             30005..30457
                     /gene="rplI"
                     /locus_tag="IXF88_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1507085.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30731..32098
                     /gene="dnaB"
                     /locus_tag="IXF88_000325"
     CDS             30731..32098
                     /gene="dnaB"
                     /locus_tag="IXF88_000325"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1507086.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32878..34170
                     /locus_tag="IXF88_000326"
     CDS             32878..34170
                     /locus_tag="IXF88_000326"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1507087.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            34957..35820
                     /locus_tag="IXF88_000327"
     CDS             34957..35820
                     /locus_tag="IXF88_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1507088.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(35949..37571)
                     /locus_tag="IXF88_000328"
     CDS             complement(35949..37571)
                     /locus_tag="IXF88_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1507089.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            37820..38341
                     /locus_tag="IXF88_000329"
     CDS             37820..38341
                     /locus_tag="IXF88_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507090.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38527..40257)
                     /gene="spxB"
                     /locus_tag="IXF88_000330"
     CDS             complement(38527..40257)
                     /gene="spxB"
                     /locus_tag="IXF88_000330"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1507091.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40422..41240
                     /locus_tag="IXF88_000331"
     CDS             40422..41240
                     /locus_tag="IXF88_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1507092.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFDAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41343..41540)
                     /locus_tag="IXF88_000332"
     CDS             complement(41343..41540)
                     /locus_tag="IXF88_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507093.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41709..42287
                     /locus_tag="IXF88_000333"
     CDS             41709..42287
                     /locus_tag="IXF88_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1507094.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT        12985 a         8199 c         8456 g        13069 t
ORIGIN      
        1 tgaaagtcaa taagaaaatg cttatttatc aacatttcta gcttacatga tcattggtta
       61 gttctggtcc tttttcgttc gtctttataa ctccgagaga cgtaaaatcc ttttgttttc
      121 agtttaatgt tcatgttctt gtcttattca caccaactag atgtctcacc ttctaaaatt
      181 ttccttaaca aagtgctgct tttatctatt tctgcttttt tttgctagaa taatagtaac
      241 aatatgactc tggaggattt atttatgaaa attttgattg ctgacgatga taaagaaatc
      301 gttgaattat taagtatcta tgttcacaat gaggggtatg aagcagtaaa ggcttatgat
      361 gggaaagaag cattatctaa aatcagaaca atccctgata ttgaattact tattttagat
      421 atcatgatgc ctgagatgga tgggatgcaa gtagtgaaag agctacgaaa agaatcccaa
      481 ataccaatca ttatgctgac agcaaaaact actgatatgg ataagatcaa aggactggtc
      541 gctggtgcag atgattatgt gaccaagcca tttaatccgt tagaagtaat ggctagagta
      601 aagtctttat tacggcgtac aaaaatgcag attgccccag atcagcctga tgtattagaa
      661 gtaaatgcat taatcatcaa taaagactct catgaagtta aaacaatcga tgggaaagaa
      721 atccagttaa cagctttgga attcggtatc ctctacttgc ttgctagtca tcctaatcga
      781 gtatttagcg cggatgaaat ttttgagcgt gtttggaaac aagaaagtat tgtctctgct
      841 aaaactgtca tggttcacgt tagtcattta agagataaga ttgaagaagc aactggtggt
      901 gaaaaagtca ttcaaaccgt ttggggagtg ggctataaaa ttgacgcaag atagtaaacg
      961 agcaactgaa aaacgccgtc ggatcaattt gacttcaaaa gaaatcagcg aattgttagc
     1021 ggaaggaatc atcacgatta tccttttgct tctattaaat gtttctattc tggttgttat
     1081 cagttcggtg atcaacagtt caccttcttt gacgaatgcg atttgggatt caaagaatat
     1141 ttttgcagaa cgattgaaca ctgatttgtt ctggaacggc cggaatttta ttattccctt
     1201 tttctttcta ttagatattg gtgttttgta ttggcgcttg attcgaagat atcgccaaat
     1261 gcaattgcgg catatcatca gcgaacttca ctacatcgca aacggaaatt atgatcatcg
     1321 tatccctttt gaactgagtg gtgacttaag ccgagtggta acaagtatca atggattggt
     1381 ggatagtacc gtagcagcga ttgaagacga acgtaaaatc gaaaaatcaa aagatgaatt
     1441 gatcacgaat gtcagtcatg atatccgtac cccactcacg tccatcattg gatacctagg
     1501 actgatagaa gatggccagt atcactcaga agaagatctt ttaaagtata cgcatactgc
     1561 ttatatcaaa gcaaagcaga tgaaatcttt agtggacgac ttatttgaat acactaaggt
     1621 acgccagcct gctgtcccgg tgaatttttc tgcctttgac atgatccagt tgatcgaaca
     1681 gttagcagct gattttgagt tggaagcttc taaaaaaaat atccaaatac ttgttcaatc
     1741 aaaagtagac tcccttatca tggatggcga taccgaaaaa cttgtacgtg tcttcaataa
     1801 tcttttgacg aatgcgttga aatacggaaa aggagcaacg aagatcgtaa tcgaagtaga
     1861 acgtatcggt tcagaagttg ttgcaacagt caaaaataac ggagcaatga tcccacaaca
     1921 ggcaatcgat aatctattcg accgttttta tcgtgtagaa gaatctcgtt cacaagcaac
     1981 tggaggaact ggacttggtt tagcaatcgc tcaaagcatt gtggctttgc acggcggata
     2041 catttatgca aaatcagata agcaatggac ttctttcatc atgcaccttc caatcaagaa
     2101 aaatgaaaaa ttacctattg atacacagga agttattgac gaaagctgaa atattcgcta
     2161 ttcttattac aattattaga ataaatgaaa aacacagaac ccagtacact ttattctttt
     2221 tcagagagct gatggcaggt gtaaatcagc aaaggataat ttggaagttc cagttcgttt
     2281 acgtgccctt tcgaaataag aaaggtcggt tcaacagccg ttatgtgttc aacaagtgca
     2341 gctgacgctg aacttgggtg gtaccgcgaa tttcagactg attcgtccca agaataatgg
     2401 gatggatcag tcttttttta ttttcaaaaa ggaggaaatc atatgttaga tgtaaaaatg
     2461 atgcgacaaa atattgaaga agtacaagaa aaattgatga ctcggggcgt aaaaaaagaa
     2521 acattagctg aattcatgga actagatgaa aatcgtcgta agctactagt caaaagtgaa
     2581 gaattaaaaa aatatcgaaa tgatgtttct gctgagatcg cccaattgaa gcgtgcaaaa
     2641 gaagatgctt ctgctaaaat cgctgaaatg aaagaagtcg gtggcaacat caaagcattg
     2701 gatactgaaa ttgctgaagt ggatgaaaaa ttgaaacaaa ttgcaactac tttgccaaac
     2761 cttccccatg attctgtgcc agttggaaca gatgaagagg acaatgtaga aatccgtcgt
     2821 tggagcgaac cacgtacatt tgcttttgaa ccaaaaccac actgggaaat tgcagaaaat
     2881 cttgatattt tagattttga acgtggcgca aaagtagccg gtagccgttt tgtctattac
     2941 aaaggattag gtgctcgatt agaacgtgct gtctacaact tcatgcttga tcagcatatt
     3001 tacgaacacg gctatactga aatgatcact ccttatatcg tcaacagtaa agcgatgttt
     3061 ggtacgggac aatttccaaa attcaaagaa gatgttttcc aattggagaa tactgatcta
     3121 acattaatcc caacagctga agtaccatta actaactatt ataatgatga aattttagat
     3181 ggtgcagatt tacctattta tttcactgca ctaagccctg cttttcgttc tgaagccgga
     3241 agtgctggtc gggatacacg aggactgatt cgtcttcacc aattcaataa agtagaaatg
     3301 gtcaaatttt cggatgtaga gcattcttac gaagaattgg aaaaaatgac agctgatgca
     3361 gaagatatct tgaaaaaatt gcatttgcct tatcgtgtga tggccttatc aactggagac
     3421 atgggctttt ctgcagctaa aacttatgac ttagaagtat ggatacctgc acaagatact
     3481 tatcgtgaaa tcagttcttg ttctaactgt gaagatttcc aagcacgccg cgcaatgatc
     3541 cgttatcgcg ataatgatgg aaaaatccac tatactcata ctctgaatgg ttcaggactt
     3601 gcagttggcc gtacagtagc tgccattttg gaaaactacc aaaatgaaga tggttctgtc
     3661 acagttccag aagcgctcgt tccttatatg gggaatctga aagttattaa ataaagaggc
     3721 tgtgaaaaag ccgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca
     3781 tttttgcata aattggactt aataccgaga aactggcttt tgaacaccat ttatcgagag
     3841 gttgtgaaat cagcgaatta acctgagtat tagagaaaaa aatggaaaaa tagtccctca
     3901 tattttaccg tttgttgaac taataccgag ggtcagctga ttgaacaccg tttagtggag
     3961 aggtcgtaag cctacccccc agctcctagt tacaaggaca aattcaaaaa acagctcttc
     4021 atattttatg aatgttgtac ttgtgattcg gaggtggcaa gggaacactg tttattcaaa
     4081 aagaggctgg gacagaagcg tttaactcca agaaataaga agaaattcac gaaaattgct
     4141 tttcaaattt ttgtgaattt cttcttattt ccgaaggagt tgcttctact tccgccgttt
     4201 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatgt
     4261 ttttaagatt caactgaata gtttggtgct tcttttgtga tttgtacatc atgagggtgt
     4321 gattctttta atccactgcc gctcatttgg ataaattgcg cttcatcacg taattgctgt
     4381 agattggctg ccccaacata acccattcct gaacgcaaac cgccgatcat ttggaaaata
     4441 atatctgcaa cacttccttt ataagcaacc cgaccttcga ttccttctgg tactaattta
     4501 tttgcttcat tgacactacc ttggaagtaa cggtcttttg aacctttttc catcgcgcct
     4561 aatgagccca tacctcggta tgttttgaat cgacgtcctt ggaagatttc gaattcacct
     4621 ggtgattcat ctgtccctgc caacatactt cctagcatca ctgcatgtcc gccggcagct
     4681 aatgctttga cgatgtcacc agaatatttg atccctccgt ctgcaataat cgctttgcca
     4741 tattgacgag caactgatgc tgcatcataa atagctgtga gctgaggtac acctacaccg
     4801 gctaccacac gtgtcgtaca aatcgatcct ggtccgatac caacttttac gacatctaca
     4861 cctgcatcat ataaagcttt tgtcgcttca gccgtcgcaa cgtttcctgc aatcaatgtt
     4921 gcatctgcaa aagttgaacg gatttcttga atttttcgga tcacacctgc actatgtcca
     4981 tgagctgtat cgatgatgat tgcatctgcc cctgcttcta ataaagcatt cgcacgctca
     5041 aatgtatcgc ttgttacacc aacggctgct gctacaagca atcgaccatg ttcatctttt
     5101 gctgcatttg gaaattcgat cactttctcg atgtctttga tcgtgatcaa tccgcttaaa
     5161 cgaccttcgt tatctacgat tggtaatttt tcgattttat gtttttgcaa aatcttttct
     5221 gcatctttta gagaggtacc tactggggca gtcactaagt tgtcttttgt catcacatca
     5281 gcaatcggca tgtgataatc tgttacaaaa cgcatgtcgc ggtttgtaat tatccctacc
     5341 aattttctgt tttcaagtgt ttcaacgatt ggcacaccac tgatacgata tttgctcatc
     5401 aattcttctg cgtctgctac aagatgctgt ggcgttaaga agaacggatc aatgattacg
     5461 ccactttcag aacgtttgac ttttcttact tcatccgctt gctggctgat cgtcatgttt
     5521 ttatggatca cacctagtcc gccttgtctt gccatagcaa tggccatctt gctgtcggta
     5581 acagtatcca tacttgcact gatgattgga atattcaact taatattttt agctaattgt
     5641 acgctcatat ccacttcgtt tggtaacacg tgactttctg ctggtatcaa taagacatca
     5701 tcaaaagtaa atcctttttt cgtgaacttt gtttcccagt tggacatttt tagtaccact
     5761 cctctaattt agtttatcta ttaaaataat tgattttttc atttaagtca atgatctact
     5821 tggaatcaag ttagctttct catagaatac caatataaag cgtttacatc tgtgtgtatt
     5881 aaaagagatt gtaaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
     5941 cttctcatgt ttttgaattg gttgggttta ttaccgcaga tgcaaacttt tgaacaccgt
     6001 ttagtggaga ggctgtgaaa aagctgctta gtttcgagta gtaagaaaaa ttttgtgaaa
     6061 atggctttcc acatttttgc ataaattgga cttaataccg agaaactggc ttttgaacac
     6121 cgtttatcgt aaaaagggac tgtgacaaga cttttgtcac agtcccttac tattatttaa
     6181 ctatggataa acaaaatgag gttctttact acccctagat atctagcaaa atagctgatc
     6241 tttttgctag tttatcttct tgggacggat aaacttccaa taatcccgta ttttttcttc
     6301 aagtagtaac ggattgctaa agcagttgcc ccgataatga taactactac tggatctaat
     6361 actggattga taactggtgt caatagtgtt gtcgcagaaa acagtacgac ccaaagaagt
     6421 gtcgttccac ctaggataag caatgtctta cccattcctg gtttcttgct tcgatctgct
     6481 ccgggatatt catattgata gatgtatttg tacatcatat aaaagaccca gccaccgatt
     6541 gcagaaccta gaacgagtgt agaaaagcca taagctggcg ttgttccacg tgaaaacaat
     6601 ccggtaatcg caatcatgat acttaataga ccaagcaaca gcaacgtatt atctaaccac
     6661 atcaataaag gggtggatga attttttact tctgctggct tattgatgat tgcttccgtt
     6721 ctttcagaga ctgtcccaaa caattgacgg gctgttcttc cgcctttttg ttctgcaact
     6781 aaagtaggca gaatgtcatg aagggctttt actttttctt cttcagacaa attcgctgct
     6841 tctaaagatt ttttcaaatc aaaaatgtac tgttgatttc ttttggttaa ttttgtttct
     6901 aaactattgt tttcttttac gattgctcgt aattgttctg gttccattat ctaatagtcc
     6961 tccttagccg ttccttttct caacggtcgt cacaaaagac attctaccat attctacagc
     7021 tagtttgata gcccttttac acattaaagc ggaataacat tacgtcgccg tcttgtacga
     7081 cataatcttt tccttctaat cgaacacgtc cagcttcttt tgctgcatgc atgtttccgt
     7141 aatgatccaa gtcttcaaaa gagacagttt ctgcacggat aaatccgcgt tcgaaatctg
     7201 aatggatgat tcctgctgct tgtggtgctt tgattccttt tttgaatgtc caagcacgta
     7261 cttcttgttc cccagcagta aagtaagtag ctaagcctaa caaatcataa gcagcacgga
     7321 tcaactgatc caaacctgat tcttcgattc ctaaagcttc taaaaattcc gctttgtctt
     7381 cttcatctaa ttctgcgatt tcttcttctg cacgagcact gaccacgatc acttctgcat
     7441 tttcttctgc agcaaagttg cgcacttctt gtacataatg attcgcgtct gcatctgcta
     7501 cgtcatcttc tgagacattg gctacataaa gaattggttt tgtcgtcaat aaaaatagtg
     7561 atttaacgat tttttgttct tcttcagtaa attcgatcgt tcgggcagat tttccttctt
     7621 ccagtacagg tttgatttta tccagtacag ctagttctgc cactgcatct ttatctttgg
     7681 ttttagcaac ttttgctaca cgagtatagc gtttgttgat agattctaag tctgctaaca
     7741 ctagttccaa gttgatcgta tcaatatctt ctagtggatc aacacgtcct tctacgtgag
     7801 taatattatc atcatcaaaa cagcgaacaa cgtgacaaat agcgtctact tggcggatat
     7861 ggcttaagaa ttgatttccc aaaccttctc ctttacttgc gccttttacg attccagcaa
     7921 tatctgtaaa ttcaaaagtc gtcggaacag tttttttcgg atgtacaagt tctgttaagc
     7981 gttgcaaacg ccagtcaggt acttctacca tccctacgtt ggggtcgatc gtcgcaaagg
     8041 gatagtttgc tgcttctgct cctgcttttg tgatcgcatt aaacaaagtt gattttccta
     8101 cgttgggcaa cccaacaatt ccagcagtca atgccataaa tttattcact ctttctctgc
     8161 atttttttca attacttttt ttaatttctt ttcaaaatct cgacgggtca tcatgacgat
     8221 atgtccgcaa ttcatacatc tgattttgat atctgcgccc attcgaatga cttcccagcg
     8281 attcgtctgg caagcgtgag gttttttcat ttcaacgata tcgtgcaagt cgtacataaa
     8341 aatagcctcc tttttgttta ttcttcatca aattgaatat tcaagatatc aagaattcga
     8401 gtcaaatctt tttgagaaag atattcaatc tcgatcttac ctttcccatt tttttcttgg
     8461 atagctacac ttgtaccaaa tttatccatc aaacgatctt cgctttctcg gatataatat
     8521 ggtttttcct ttgctagcct tgggattttt ttcttttctt ttccatcatt ttgatttaac
     8581 tcagaaacaa gctgttccag ttggcggaca gttaaatttt cagttactgc acgattggct
     8641 aatttcaaga tcaatgtttt attttttaaa cctaacagcg tacgggcttg tcccatagat
     8701 aaacgctgat cttgtaccat ttccttgaca agatccggta aagaaagcaa gcgcaaataa
     8761 ttggcgatat aaggtctgct ctttcctaaa cgactagcta cttctgcttg tgttagtttc
     8821 aggtttttca tcagcatctc atatgcttct gcttcttcta acggattcag atcttctcgc
     8881 tgcaagtttt ccagcactgc tacttgcatc atcgcttctt cgtcaaagtc tctaacgata
     8941 gcagggatcg tttctttttc agctagtttg gacgctcgaa aacgtcgctc ccctgcaata
     9001 atctcatatc cttttactgc ggaacggcgt acgatgatgg gttgaaagac acctgattgt
     9061 tcaatagaat tagctagttc ctgcaaagat acttcttcaa atgttttacg gggctgatac
     9121 ggatttggtc gcagctcatt caatggtagc tggacaacgg tatcatcttt tacatccacc
     9181 gtctctaagt tttctaaatc ttgaaacagt gcatcgattc ctcggcctaa tcctttactt
     9241 ttcttcacga cttaccactt cctttgccaa tgcttggtag acttcagctc ctcgagaacg
     9301 tggatcgtag tcgatgatcg gttttccatg actaggtgct tctgataaac gaatatttct
     9361 tggaatgatc gtttcgtaaa ctttttcgcg gaagtattta cgtacttctt ctacgacttc
     9421 atttcccaga tttgttcgtg catcatacat tgtcagtaag actccctcga tctctaattc
     9481 tggattaaag tgtttttgga ctaaacgaac cgtattcagt aattggctta atccttccaa
     9541 tgcgtaatac tcacactgaa caggtatcaa aatcgaatca cttgctgtaa atgaattgat
     9601 tgtcagatgg ccaagagaag gcgggcaatc gatcaaaata taatcatact gactgcttac
     9661 ttctgctaaa gatcctttta atcttgattc tcttgccatc attgacgtca attcgatctc
     9721 tgctcctgct aattggagag tcgcaggcac aatactcaaa ttttcgtgtt cagtgatcaa
     9781 agttgcctca tcaattggca attcattgac tagaacatca tagatgtctc ttgtcacatc
     9841 aggttttctg atcccgacac cactggtagc atttccttgt gcatccatat ccactagcaa
     9901 tacttttttg ccaaggctag caagacaagc acctagattc accgtcgtcg ttgttttgcc
     9961 gacgccacct ttttggttcg caacagatat tatttgtgcc atctcttctc ctcctatttg
    10021 attggctgct tattaggcag tccgggcttt cttgggtatt tctttggggt tggtttcttt
    10081 ttttgaataa cgatgatatg ccgttgatca gagacattcg gcaaggtaac cgcctcttct
    10141 ttgaggaatt taccacctaa taaagcaatc gcttgttttg cttctgtcaa ttcctcttca
    10201 ctttttgctg ctttcaatgc ataaaaatag ccttcttgtt tagccaaggg caaacacaat
    10261 tccgctaaca cattcaaacg agcaaccgca cgagcagtga caaggtcaaa tgatgcacga
    10321 aagtgaggat tttgcccaaa tgtttctgcc cggtcatgat ataaggaagc atcgattcct
    10381 aataatcggg ttaactcaga taagaaggtt attcgtttat tcagagaatc aacgatcgtt
    10441 atctgcagtt ctggaaaggc aatttttaaa ggtatgctag gaaaccctgc ccccgaacca
    10501 acatcgcata aagataaagg ttgcgtaaag tctacttcaa atgctaatgt aagtgaatcg
    10561 taaaaatgct tcagatatac gtctttttca tccgtaatag ctgtaaggtt catcttttcg
    10621 ttccattcga ctaatgtccg ataataaata gcgaattgat tcatttgatg atcggtaagc
    10681 tctatccctt tttctgccaa ggattggcga aattcttctg gtatcataaa tattcctttc
    10741 attgtgaaac agcaattgtt tcacagattc tctccctact ttaacgcaaa atctctagac
    10801 atgcaatcct tccatatgga ttcttaaaaa tttgaaaagt aagaaaagcc gatagacttt
    10861 ttctttctag tatgactcat aaaatataca cagaagagag aactagcttt gagcaatttc
    10921 tgcaaaaact agttctctct tctatttatt attacgtatt acgctggttc actgactttc
    10981 gcaatcttcc cttgttcgat atagaccatt aggatactta ggtctgcagg gtttacgcca
    11041 ctgattcggc tagcttgggc aattgtttct ggttggattt tctgcaattt ttgttttgct
    11101 tctgttgcta atccgttgat tgctgcgtag tcgatgtttt ctggaatctt tttcgcttcc
    11161 atgcgtttta atttttctac tttttccatg gcttttttga tgtagccttc atatttgatt
    11221 tggacttcta cttgttcgat ttcttttcgt gtcaattctt cattttctgg aataaaccgt
    11281 agaagttctg cataagtaat ctcaggacgt cttaaaaagt cgcttgctag tacaccatct
    11341 tttaatggtg ccgcattaat cgttgctaaa aagtcttgga cttcttctgt tggtttgatt
    11401 cgtacattca tcaaacgttt gatttcttgt tcaactgctt cttttttctt caaataattc
    11461 ttgtattgat cttcttttac taagcctaca cggtgaccca tttctgttaa tcgcaaatct
    11521 gcattatcat gacgtaaaag cagacggtat tcagcacgtg aagtcagcaa acgataaggt
    11581 tcgttcgttc cttttgttac caaatcatcg atcatgacac cgatgtaacc gtcacttcgt
    11641 ttgatcacta aaggttcttt tccctgaact ttcaaagctg cattgatacc ggcaatcagc
    11701 ccttgtccag ccgcttcttc ataaccgctt gtaccatttg tttgtccagc tgtataaaga
    11761 ttttcaacga ctttagtttc taatgtcgga cggagctggt gtggctctac tacgtcatat
    11821 tcgatagcat atcctgtacg catcatttct actttttcta atccagcaat agaatgaagc
    11881 atgttgactt gaacatcctc tggtaaagaa gtagataaac cttgaacata gacttcttcc
    11941 gtatttaagc cttctggttc caaaaataat tgatgacgtg gtttgtctgc aaaacgcacg
    12001 atcttatctt cgatggatgg acaatagcga gcgcctaccc cttcaacgat ccctgtaaac
    12061 attggggcac gatgtaagtt gtctcgaata atttggtgag tcgtctcacc tgtataggtc
    12121 agccagcaag attcctgatc tagtttatag gcactatctg gtgtgctgaa gctaaaatga
    12181 ttcggctctt tgtcaccagg ctgctcttct gtaacagaat agtcgatcgt acttgatttt
    12241 actcgaggcg gtgttcctgt tttgaaccgt tcgatagcaa agccaagttc ttttagatga
    12301 ttcgctagtc caatagaagg ttgagagtta ttcggacctg atgaatattt caattcaccg
    12361 ataatgattt ctccgcgaag tgctgttccg gctgtaataa caactgcttt ggcccggtag
    12421 ctcgctcctg tggacgtcac tactccatga caaacaccat cttcgacgat cagttcttcg
    12481 acgattcctt gtcttagcgt caaatttggt tctttttcga tcgtatgttt catttctgtt
    12541 gaataagcat gtttgtctgc ttgtgctcgc aatgctcgga cagctgggcc ttttcctgtg
    12601 ttcaacatac gcatttggat ataggtttta tcgatattct tacccatttc tccgcctaac
    12661 gcatcgattt ctcgaacgac aacgccttta gctggtccgc ctacagaagg attacatggc
    12721 atgaatgcta ccatgtccaa atttattgtc agtaataatg tttttgatcc catacgtgca
    12781 gcagcaagtg cagcttctga accggcgtgt cctgcgccaa caacgatcac gtcatacgca
    12841 tctgcttcat aatgttccaa tcgtttgttc ctccttttat tttcctaaac aaaattgact
    12901 gaataattga gtgattagct catcttgtac actgtcgccg acaacttctc ctagatagtc
    12961 ccagcaacgc gtcatatcta tttgaacgag atccacaggc attcctgaat caatacctgc
    13021 aatgacttct tcaagtgaga gggaagcttt ttctaacaac gcgatatggc gggtattaga
    13081 aacataagta gcgtctcttt ctcctgtttc tccgctgaag aataaatcag aaatagctga
    13141 ttccaaacga tctagtccgt cgtttttagc tacggaaatt gaaaaaactg gttcattttc
    13201 gatcaatttc ttcaattttt cttgttccag ctgtgctggc aaatctgttt tatttagaag
    13261 aatgatccgt ttcaatccac ttgtcgcttc caataattgt tcatcttctg ctgtcagtgg
    13321 ttcgctttgg tttagaacaa gtaagatcaa gtctgcttct gctaatgctt ttcggcttct
    13381 ttcgaccccg atccgttcca ccacatcttc tgtttcccga attccagctg tatcgattag
    13441 cttgagcggt accccccgaa cattgacata ctcttcgatt acatctcgag tcgttcctgc
    13501 aatatccgta acgattgctt tttcttctct taacaaatgg tttaacaaac ttgatttgcc
    13561 gacatttggc cggccgatga ttgccgtact taacccttcc cgcagaacct tcccttgctt
    13621 ggatgtagca agtagcgctt ggattctttg ttggatcatc tgcgcttttt caagcaataa
    13681 cttcgtagtc aattcttcga catcatcata ttctggataa tcaatattta cttctacttg
    13741 tgctaatgtc tctaaaatct cttgtctcaa tgagcggatc aaagcagaca gattgccgtc
    13801 taactgatta agagctagtc ccattgcttt gtctgttttt gcccgaatca aatccatcac
    13861 agcttctgcc tgtgaaaggt caacacgacc attcaagaat gctcgttttg tgaattcgcc
    13921 tggctcagct aatcttgcac cttctcttaa caatagctgc aaaatttgat tgactacaac
    13981 gatcccaccg tgacaattga tttctactac atcttcccgc gtaaatgtct ttggtgctct
    14041 catcacggag accatgactt catcgacaag ctgattgctc ttgggatcaa cgatatgtcc
    14101 ataatggatc gtatgacttg gtacttctga taatcgtttg tttccagatt gataaacttt
    14161 atctgctagt tgcacagctt gatcgccact taaacgaacg atactgattg caccttctcc
    14221 aggtggggta gaaattgccg caatcgtatc aaattctaaa gtaatttcag ccattttttc
    14281 acttcctttt tcattttgcg cacaaaaaaa gtgcccactc caccctttat gcacaataaa
    14341 cggtgaactt cgcactttga ctgcttgttc ttgattaaat tctggtcata gattatcaca
    14401 cttgctataa ggaatcaagt gtttaaaaca attagatgaa gttgagcctg ttctgcttcc
    14461 attaccttat atttaattgt ccttggatgt ctctttgcgt tctttggttt attcgaagag
    14521 aagatactgt ataaacaagg ctttatcttt cttaaaaaaa acggtacact aaatggtagc
    14581 gctatctaaa ctatacactt ttgaaaggag aaaatatgtg agcccgttca gatctgctgc
    14641 attggaagaa gaaagtgacc ctcggatcaa cgatgtcgtt ttatctacag ggaatatcaa
    14701 ccagaaatat atcgtccgcg atatcgtttt tgttgtggaa tgcttagaag ctgatttgtt
    14761 tgacccatct tttcccaaag atgacctttt cttaacaacg aagaggaaat taaagacgaa
    14821 ggtacttgaa tatggtgcga atgcagtaat caactgtcat tttgagtatc agcaaaaaaa
    14881 acgcgacgga aagtattttc tggaactttt tgcttatgga acagtgatcc aatttatcca
    14941 aacaacgatt ggttaaatga aacagacaaa aaaagcggat caaaactgaa gacagtttcg
    15001 atccgctttt ttctatcaaa gttagtgact acttcatcgt atactctgat aagaatgatt
    15061 tctagtaaaa tatacttggt ttgaaaaact ttgtcaacgt gatttattca aaaagtaatg
    15121 gaatcaataa tattttttag caggttctac tactaaataa cgataaggtt cctcaccttc
    15181 cgaatgtgtt tttacatgtt catctttgct taatgcagca tggatttgtt tacgttcaaa
    15241 tgcaggcatt ggttctaaga aaacaggctg ccctgtccgt tttacttttt cagccgtgcg
    15301 atcagctaaa cgctgaatga tctcttggcg cttttcacgg taattgccta cattgactac
    15361 tacagacagc ttatttcttg ctaaacgatg gatgaagact tgtgccagat attgcaatgc
    15421 attcagtgtt tttccatgtt tcccaatcag tatgccttgc ttttgtgttt ctaaatggaa
    15481 aacaaccata ttatttgatt ctctttccat tttgacaaga gctggagcat caagctgttt
    15541 tgaaatttca gtaagataaa ctgccaattc cgttaaagct gcttcatcac ctagctcact
    15601 tacttcttct gttttttctt caagaatcgt ttcgttgact acctcgatct cagctgttgg
    15661 ttcttcttcc aaaatttctt ctacagtctg ttctactgtt tcagaaactt tttcgctgat
    15721 acttggctgg atcgaaagcc gtgcattctt tcgacccatt cctaggaaac ctttttttcc
    15781 ttcttccagt atttcgattt cgacttggtc tttggccaaa cccaaatcag ccagacctag
    15841 ttggacagct tcgtctattg ttgcagcttc ataaatcggc attagcatta cctcccctat
    15901 tatttccgtt ttttcttagg attcattgct ttcttaagag cacgttctct ttcgcggatt
    15961 ctccgtgctt cttcttcacg ctcttttcga attttgaaag ggttgttgat cagcagcgtt
    16021 tgtacaactt ggaaggcatt ggaaactacc cagtataatg aaagaccgct ggctaaattg
    16081 acacccatca ctaaaatcat tacaggcatc acgaaattca tgatcttcat tgatgcattc
    16141 gattcaattt gactcatgct agataaatac gtacttgcaa aagtaaagat tgctgctaaa
    16201 atcggcaaaa taaatgttgg atccgcgttc cctaagttca accataagaa atgcccttgt
    16261 ttcaactctg gtacacgtga aatagattgc cataacgcca tcaagattgg catttgtact
    16321 aataaaggaa gacaaccagc atatggattg acgccatttt ctgcatataa acgctgctgc
    16381 tcttctctta acttactttg tgtttccgga tctcttgaac tatattgttg ctgcagtgct
    16441 tttaatttcg gttgtaattc ttgcgtttta cgcatgctct ttgtttggaa atgcatcaac
    16501 ggcaatagga tcacgcggat gatcagtgta aataaaatga tccctatccc tgcattacca
    16561 aatgacaacg ccttgatcgc ctcagcaaag taataaacaa tataacgatc ccaaattcct
    16621 gtactctgtg cgctgacttc cccagttccg caaccggata agacgatgac caaactaacg
    16681 ataccggcca tcaaaagtaa acgtttgtat ttcttcactt actcttttcc tctctgtcga
    16741 ttatttttgc aagttttaag acgtggacta aattggagcg aacctcatca taagtcaacc
    16801 ccttcacact tggtctagca atcacaataa aatccaactc cggatcaatt tcttctttta
    16861 tagaataaac tgctgctcgg atttttcgtt tgacttcatt gcggtttacc gcgttgccga
    16921 ccttttttcc tacagataaa ccaactcgaa aatgaggctg tcccggtttt tccaaacggt
    16981 aaacgacgaa tttgcggtta gcaaaagaat ctcccgtatg gaacacggct tgaaactctc
    17041 gttctttttt tactcgatac gcttttctca ttagttttct ttccttttct atctctcact
    17101 tacgctcata atcataccat aattacgatt gtcctttcat catttcccta aaaaagagta
    17161 taaaaaaaac cactgaaacc ccagtggcct aagctgaaag aacttttctt cctttgcgac
    17221 gacggctagc taacacacga cgtccatttt tagtactcat acgtttacgg aatccgtgaa
    17281 ctttttgacg tttacgttta tttggttggt atgttctttt catatattcc acctccaaaa
    17341 tgttcttatc tattttataa acacagacat actttgatag tataacgaca aagtagtgat
    17401 tttgtcaata taaaatagaa gatcgattgt taaaacttat ccacaagatt atttttttct
    17461 atgggaatcc tatgtggaaa aagttatgca cagggattat ccacagataa taaaaggctg
    17521 gttttacaca gaaaacacac gtgtaaaaaa agtaatccac aatcaggcga tttcctgtgt
    17581 attccgtctc ctaactattg gtatatctgt attttttatc cacagacgca agaggaaatc
    17641 tattggtttt tacgagtttt ccacctgtgc attagtggtg tggaaaactt tttttgacaa
    17701 ctgtgttttt ctcttttttc tttttgtcga ttgtgtggat aattgtaatt aaaaaatttt
    17761 tttcttcggt ttctgtggaa aactattgaa aaaaatgcta aattagtact agttattcac
    17821 tttttaatga aggaggaggc cttatatggt atccctcgat gctttatgga atgaattaaa
    17881 agcaacatac caaaaggatt tatcgcctgc tagctataat acatggatcg aaacagcgaa
    17941 ccctcgaacc cttgatcaaa accagctcgt tgttgaggtt cctagcaaga ttcataaaga
    18001 atattgggaa aaaaacctgg cgacaaaaat cgttgaagtc ggttatatgt tatcaggaaa
    18061 tgagattatc cctcgcttta ttactggcga agaagcagag caagaagaag ttatagaaga
    18121 aaaaaatcca aaagttgtgg cacctagtcc gctaaaaaaa gccatgctga accctaaata
    18181 tacctttgat acgtttgtca ttggtaaagg aaaccagatg gctcatgcag cagcgctcgt
    18241 tgtagcagaa gatccaggtt ctatttataa cccgctcttc ttttatggag gcgtgggact
    18301 tggtaagact cacttgatgc atgcaattgg tcatcaaatg ctgcaaagtc agcctaatgc
    18361 caaagtaaaa tatgtaagca gtgaaacttt tgctaatgat ttcatcaatt cgatccaaaa
    18421 caaaacagca gaagagttcc gtcaagaata tcgaaatgtt gatttactgc tagtggatga
    18481 cattcaattt tttgcagaaa aagaagctac gcaagaagag tttttccata ctttcaatgc
    18541 cttgtacaat gaagggaaac aaatcgtact aaccagtgat cggttgccaa acgagattcc
    18601 gaaactgcaa gaacgccttg tttctcgctt tgcatggggg ctgtctgttg atattacacc
    18661 gccggatctt gaaacaagaa cagcgatttt acgtaaaaaa gcggcggctg aacgtctaga
    18721 gatcccagat gacacactaa gctacatcgc aggacaaatt gattctaaca ttcgtgaatt
    18781 agaaggtgcg cttgttcgag tgcaagcttt tgctgcgatg aatagtgaag atatttcgac
    18841 tagtctagct gcagacgcac tgaaaacgtt aaaatcagga aaaggacatc ctcaattgtc
    18901 aatcctgcag atccaagaag aagtggcaaa gtattatcac atccaattga aagatctcaa
    18961 aggaaagaaa cgcgtcaaat ctattgttgt tcctagacaa atcgctatgt atttggcacg
    19021 tgagctcaca gataattctt taccgaaaat cggagctgaa ttcggtggca aggatcatac
    19081 aacggttatc catgctcatg aaaaaatcca gcagctgatg gattctagtg tcagtatgca
    19141 aaatgaagta tcagaaatca aaaacttttt actaaattaa tttgtcgatt ctatcaactg
    19201 aaaatagcat gtggataaaa gaaaaaaacg ttcaaaagtt atacacactt tttccacagc
    19261 tgttttttcg tttctctctt acgttttttg agttttccac agaatcaaca tgccctatta
    19321 cttttattat tctttttaac taataataaa taaacaaaca ttcaaaggag atacatcatg
    19381 aaagttactt taaaccgagc tagctttatg caggaattgc aaactgttca acgagctatt
    19441 tcaagcaaaa ccacgatccc tattttgaca ggtgtaaaaa tcacactgac acaagaaggt
    19501 ttgactttga cggggagcaa cgctgatata tcaattgaaa cttttttgtc tgttgaaaac
    19561 gaaaaagcaa atatgcaaat cgaatctact ggttccattg ttttacaagc acgtttcttt
    19621 agcgaaatca ttcggagact tcctgaagaa acatttactt tagaagtttt agaaaataaa
    19681 caagtagcga tcacttctgg aaaagcgaat tttatcgtaa atggattaga tgcagataac
    19741 tatcctcatc ttcctgttgt cgaaagccat aaccagatga aattacctgt acacgtattg
    19801 actaaactaa tcaacgaaac agtttttgct gtctcacaac atgagagtcg tccaatcttg
    19861 acaggtgtgc atttcatttt atctgataat tctttattag ctgtagctac tgattctcac
    19921 cgtctaagtc aacgcgtgat tccagtagaa caagcggctg atcattttga tattgttatt
    19981 cctggaaaaa gtttgatcga attatctcgc tcattaacaa atgaagaaga aatcgtcgaa
    20041 atcagcatta tggaaaacca agtgttgttc aaaacagaaa cgatgtattt ctattcccgt
    20101 ttgctagaag gaaactatcc agataccaat cgtttgattc cttcaagttt taatacagag
    20161 gttgaatttt ctgttcctag ctttttagcg gcgatcgaac gggcttcttt actttctcat
    20221 gaaggccgta acaacatcgt tcgtttatct attcgtccag atgcggttgt tttatacgga
    20281 aattcgccag aaatcgggaa agtagaagaa agcttgagtt atacagcaag cagtggcgat
    20341 ccattagata tttctttcaa tccggattat atgaaagcag cgttgcgtgc atttggagat
    20401 atgagcatca aagtgaaatt tatctctgct attcgtccat ttacattgga accaacggaa
    20461 gatggcgttc aatttattca gctgattact cctgtacgta caaattaaca atgattttca
    20521 atgttcttga aaatttaatt gaaaagaaat agaaaaaata aaattttaaa cagaaggccc
    20581 taaaatctct tttaaggctt tctgtttatt tttgtttaaa tcaccttttt tagattaaat
    20641 cgtcttatat ggaaaaatat atggataaat ttgttttatt gcgaaaaaaa aggtataata
    20701 gagtatactt ttaataatga aaatgaggga tgggcattga aacaacaagt cattctaaat
    20761 acggaattca tgacgttggg ccaaatgttg aaagaagtca cagttatcgg tagtggcgga
    20821 caagcaaaat ggtatttggc agaaaacact gtgttagtcg atggcgaacc agaaaatcgt
    20881 cgtggacgca aactttatcc cggcatgatg gttgaagttc cagaagtagg aacttttttt
    20941 atggtaaaaa aaggaaacga cgataatgag gctgaataag ctgtatttaa aaaattatcg
    21001 aaattacgaa gagctgaaca tcgaattttc caagaacctc gtcatctttt taggagaaaa
    21061 tgcgcaagga aaaacgaatc ttttggaaag tatctacgta ttagctatga cgcgaagtca
    21121 tcggacaagt agtgagcagg aattgattgg ctggactgac gatcaggcga tgattcaagg
    21181 agagatcaca aaaggttcat ccactcttcc attagaaatc ttattatcta aaaaaggccg
    21241 aaaaaccaag atcaaccata tcgaacagaa aaagttaagt acttacgtgg gacaaatgaa
    21301 tgtcatttta tttgcaccag aagatctttc tttagtaaaa ggcagcccgc agatcaggcg
    21361 gaagttttta gatatggaat taggccagat caatccaatc tatctttatg atcttgtcca
    21421 ataccaagca attttgaaac aaagaaacca gtacttgaag caattgaatg aaaaaaaaca
    21481 aactgatttg ctttatttgg atattttgtc cgaacaacta gcagcgtttg gcagcaaagt
    21541 attgaaagca agagcacagt tcgtcaagcg attggagttt tgggctaatt ctttgcatca
    21601 gcaaatcacc catcaaaaag agcagctgga aatcgagtat ctcaccgcag tcgattcttt
    21661 agaaacacat acgcaagagc aaatccaaga acaatttctc gcacttttga accaaaataa
    21721 aaagaaagac ctttttcggg gaacaacgac agttggtcct catcgtgacg atctttcctt
    21781 ttttattaat cagaaaaatg tgcagactta tggatcgcaa ggacagcaaa gaacaactgc
    21841 actaagtgtc aaattagcag aaatcgattt gattaaagaa gagacagggg aatatccaat
    21901 ccttttgtta gatgatgtga tgagcgagct ggatgataat cgtcaactcc atctgctaga
    21961 aacaatcgaa ggaaaagttc aaacgttctt aacaacaacg acacttgacc atgtaaaaaa
    22021 taaaatgact gttgaacctg agatttttta tgttcaacaa gggaaactaa aaggagaaga
    22081 aactgaatga cagaagaaag aagtttagta gaacgcgcta aagagtatga tgccagtcag
    22141 attcaagtac ttgaaggact tgaagccgtt cgtaaacgac ctggcatgta tatcggttcg
    22201 actagttctg agggtctgca tcaccttgtt tgggaaattg tcgataattc gatcgatgaa
    22261 gtattagcag gttttgcaac gaaaatccat gtgattattg aaaaagacaa cagtatcacc
    22321 gttattgacg acggtcgggg gatacctgta gatatccagg caaaaactgg acgtcctgca
    22381 gttgaaacgg tctttactgt tttacatgct ggaggaaaat tcggcggtgg tgggtacaaa
    22441 gtttctggtg gacttcacgg agtaggatct tctgttgtaa acgcgctttc tacacagctt
    22501 gatgtcaaag tatacaaaga aggaaatgtg tactatcaag aatatcgtcg tggagctgta
    22561 gtggacgact taaagattat cgaacaaacg gatcgccatg gaacaacggt tcactttacg
    22621 ccagaccctg aaatttttac agagacaact gagtttgatt tttcaaaatt ggctacccgg
    22681 atccgtgaac ttgctttctt gaaccgcggg atgcgtatat cgattgaaga caaacgagaa
    22741 gaagaaccag tcatcaatga ataccattat gacggcggga tcaaaagtta tgtcgaatac
    22801 ttgaatgcaa acaaaaccgt gatttttcca gaacctgtat atttggaagg tgaacagcaa
    22861 gatatcgcag ttgaagtctc tatgcaatat acagacggct accattccaa tatcatgagt
    22921 tttgccaaca atatccatac gtatgaaggt ggaacacatg aatcaggatt caagactgct
    22981 ttaactcgtg tgataaatga ttatgcccga aaacaaaagc tgatgaaaga aaatgacgac
    23041 aacttgaccg gtgaagacgt gcgagaagga ttgacagctg ttatttctat caagcatcct
    23101 gaccctcaat tcgaaggaca aacaaaaaca aaattgggca attccgaagt acggacagtc
    23161 actgatcggt tatttagcga acatttcatg aaattcttgc tggaaaaccc aagtgtgggc
    23221 cggcaaattg ttgaaaaagg attactagct tccagagctc gtctagcagc gaaacgtgcg
    23281 cgtgaagtaa cgagacgtaa aggtgcttta gagattagta atctgccagg aaaattagcg
    23341 gactgctcta gcaacgatcc tgaaaaatgc gaattgttca tcgtcgaagg agattcggcc
    23401 ggcggatcag ctaagcaagg tcgtaatcga gagtttcaag ccatcttgcc tattcgtggg
    23461 aaaatcttga atgttgaaaa agcaagcatg gacaagatct tggcaaatga agagatccgg
    23521 tctttattta ctgctatggg tacagggttt ggagaagatt ttgatgtatc gaaagctcgc
    23581 taccacaaat tagttatcat gactgatgcc gatgtcgacg gcgcgcatat ccgtacattg
    23641 ctgctgactc ttttttatcg ttacatgcgt cctattgttg aagctggata cgtctatatt
    23701 gctcagccac ctttatacgg ggtaaaacaa gggaagaata ttacgtatgt acaaccagga
    23761 aaaaatgcag aagaagagtt gaaacaagtc gttgcttcac ttcctgcctc accaaaacca
    23821 agcgttcagc gttataaagg acttggagaa atggatgatc atcaactatg ggaaacaact
    23881 atggacccaa gcaatcggat gatggcccgc gtcagtgtgg acgatgctat tgcagcagat
    23941 cagatatttg aaatgttgat gggggatcga gtagaacccc gtcgtgcatt catcgaggaa
    24001 aatgcacact atgtgaagaa cttagatatt taatcatgaa ggaggaaaaa gaatgagtga
    24061 agaaatcaga gaaaacatcc atgatgtcaa tctaaccagc gaaatgaaag actccttcat
    24121 cgattatgcg atgagtgtta ttgtcgcacg tgcacttcct gatgtcagag atggtctgaa
    24181 acccgttcat cgccgtattt tatacggcat gaacgaatta ggtgtaacac cggataaagc
    24241 ccataaaaaa tctgccagaa tcgtcggaga tgtcatgggt aaataccatc ctcatggaga
    24301 cattgcgatc tatgagtcaa tggtacggat ggcacagcca ttcagctacc gttatatgct
    24361 agtggatggt catggaaact ttggttctgt tgacggtgac ggtgccgccg ctatgcgtta
    24421 taccgaagca cggatgagca aaatcgctac agaaatgctg cgtgatatca ataagaacac
    24481 tgttgatttc caaagcaact atgatgatac agaaaaagaa ccagttgtac tacctgctcg
    24541 cttcccgaat cttttagtga acggaacaac ggggatcgcc gtaggtatgg ccacgaatat
    24601 cccacctcat aacttgtctg aggtagtagc ggctatcgat ttgctgatgg ataatcctga
    24661 tgtgacgacc aatgaattga tggaagtatt acctggacca gattttccaa caggtggttt
    24721 ggttatgggg aaatcaggaa tccgaagagc gtatgaaaca ggtaagggtt ctattaccgt
    24781 acgtgcaaaa gttgagatta ctgagatgcc aaatggtaaa gaacggatca tcgtgacaga
    24841 attgccttat atggtcaata aagcaaaatt gatcgaacgg atttctgaac tgcacagaga
    24901 caagcgtatc gaagggatca cagatttgcg ggacgaatct tcccgtgaag gtatgcggat
    24961 cgtcattgac atccgtcgtg acgccagcgc atccgttatc ttgaataact tatataaatt
    25021 gacatccttg caaaattcat ttggattcaa tatgttggcg atcgaaaaag gtgtgccgaa
    25081 agttttgagc ttgaaacaaa tccttgaaaa ctatatcgaa catcaacgcg aagtcatcac
    25141 acgacggacg gtctttgaaa aagaaaaagc agaagcaaga gcacacatct tagaaggttt
    25201 gcgtattgct ttggatcata tcgatgaaat cattgcgatt atccgcgggt ctaaatcaga
    25261 tgacgaggca aaagcaacaa tgatcgaacg gttcgattta tccgatcgcc agtctcaagc
    25321 tattttagat atgcgtttac gccgattgac cggtttggaa cgggaaaaaa tcgaaaatga
    25381 ataccaagaa ctcttaaaac tgattgaaga tttaactgac attctggcaa gaccagaacg
    25441 tgttacagaa atcatcaaga atgaattagc agacttgaat caacgatttg gtgataaacg
    25501 acgcacagaa ttactagtcg gtgaggtctt aagtctggaa gatgaagatt taatcgaaga
    25561 agaagaaatc gttattacat tgacaaacaa tggttacatc aaacgtttgg caaataatga
    25621 attcagagct caacgaaggg gtggacgtgg tgtccaagga atgggtatcc acaatgacga
    25681 cttcgtcaaa aatctggttt cttgttctac ccatgataca ttgttattct ttacgaataa
    25741 cggaaaagtg tatcgggcaa aaggatacga aattccagaa tacgggcgta cagccaaagg
    25801 aatcccaatc atcaatttac taggtatcga ttcgagtgaa agcatccagg caatcattgc
    25861 ggtgacggga gaagcagaag aaggccacta tctcttcttt acgacgcgtc aaggtacagt
    25921 aaaacgtact tctgtcactg cttttgctaa catccgaagt aatggtctga tcgcaattgg
    25981 tttgaaagaa gacgatgagc tcgtcaatgt gctactgaca gatggtcaat ctaatatcat
    26041 tattggaaca cataacggct actctgtcac tttcaaagaa gaagcagtcc gcgatatggg
    26101 acgtacagct tccggggtca gagggatacg cctgcgtgag gaagattatg tcgttggggc
    26161 agccttaatg aaggaaaatc aagaagtatt agtgatcaca gaaaaaggtt acggaaaacg
    26221 gacaaaagta tcggaatatc cagtgaaagg ccgcggaggt aaagggatca aaacggcgaa
    26281 catcactgaa aaaaatggtc cactagctgg attgaccact gtttctggag atgaagacat
    26341 catgctgatt acagataaag gtgtcattat ccgtttcaac gtatcgactg tttctcaaac
    26401 tggacgttca acattaggcg tacgtttgat gaagatggaa gctgatacga aagttgtcac
    26461 gatggcagca gtcgaaccag aagctgcaga acaaacagtt gaagaacaag gatcagaaga
    26521 ataaccatca agactaagtg tgtaagctag caagaataac cgattaaaaa atctggttat
    26581 tcctgctggc ttttttgatt agttgaattt ctcttaaaat attcgaaact gttcattctg
    26641 tattgctttt gaccaaaata gagtgtataa ttagagcttg tgagtagtct aattaattag
    26701 ataactctcc ttgctcttag gaacctaaga gccgaaagtc cacagggagg tgaatatcaa
    26761 tggaaaacac gaaatatgaa atcatgtaca ttattcgtcc aaacattgat gaagaagcaa
    26821 aaactgcttt aatcgaacgt ttcgatgcaa tcttgaaaga taacggagca gaagttatcg
    26881 aatcaaaaga ttgggaaaaa cgccgtttcg cttatgaaat gaacggttac cgtgaaggta
    26941 tctatcacat cgttaaagta tcttctccat catcagcaaa tgctgtcaat gaatttgacc
    27001 gtcttgctaa aatcaacgac gatatcattc gtcacatgat tgttaaagaa gaagcttaat
    27061 tgttgtttca tgtgaaacaa tagaaaacga tgagaggaga tgactatttt gattaataat
    27121 gttgtattag ttggacgtct gacgaaagat ccagatttgc gttacacagc aagtggtacg
    27181 gcagtcgcaa catttacttt ggctgtgaat cgtaatttta caaaccaaag cggcaatcgc
    27241 gaggctgatt ttatcaactg tgtgatttgg cgcaaatcag cagaaacttt agcaaactat
    27301 gctcgtaaag gaacactttt aggtgttact ggacgtatcc aaactcgttc ttatgataat
    27361 cagcaaggac aacgtgtata cgtgacagaa gtagtagctg acaacttcca attgctggaa
    27421 agtcgttctg catctgaaaa tcgccaacaa agcggcggat tccaaagttc tggtcaatca
    27481 gctggcggat ttggcggaaa caataactct aatcaaacat ctcaatcatc caacggtatg
    27541 cctgatttcg accgtgatac ttctgaccca ttcggctcaa gttcaacgat cgacatttca
    27601 gacgatgatt taccattcta aacgaatttg ataggaggga aatagaatgg cacaacaaag
    27661 aagaggcgga cgtaaacgtc gtaaagtaga ctacattgct gctaaccata ttgaatatat
    27721 cgattataaa gatatcgaat tactaaaaag atttatttca gaacgcggta aaatcttgcc
    27781 acgtcgtgta actggtacag gcgctaaaaa ccaacgtaaa attactgttg caatcaaacg
    27841 cgctcgtatc atgggcttgc tacctttcgt tagcgaagac taataaatcc taggaattga
    27901 atgactgatc cttttgatca gctattcaat tccttttttt ctgtaaaaat aacctctatt
    27961 cgtttagtcg ctcgaatgac gaaagttatg gtaaaatcaa attagtcata tatgggagga
    28021 aaattatgga aaataaaaac aatcatttat ccccgaaacc tctcttcctt atcctgttta
    28081 tattaatcga agtattggta gtcttaattt taccatttcg ttttgttagc gcttcggtca
    28141 ttctattgat gaatttatgg ggtgttcgct attggttgaa aatgaaacgt tggcttattt
    28201 taaataaacg tgaaaaaatc cagcaggctt cgactgtagc tgaaaccaat ctagcttatg
    28261 tctcagaagt catgcctgta ggagtgattg cttatcagcc atcaagccaa aaaatcgaat
    28321 ggctcaatcc attagcattg aaaatcaaag atcaaagtgg attaacagag gaagaattgc
    28381 tagatacttt ttttactgca aaagaaaaaa agaaaaacca agtctatcta gcagataaaa
    28441 catttcagtt tgatatggat acagtaaagg gtgttgttta ttttaatgat attacccaaa
    28501 accagttgtt aaaactccat cagacgaaca gtcagccggt gattggtatt ttatctattg
    28561 ataactatgc agatgccatt gataaattag atgacaaaga gatttcttat ttgaactctt
    28621 tcgtcactac actagtttct gactggatga atgaatacca tgtctttttc aaaagaatca
    28681 acgcagaacg gtttttcttc gtagcacaga tgatcgatct gaaaaagatg atggataaaa
    28741 aatttgattt gctggatcgc tttcgagcag aagctgaaaa acagagctcg cctatcaccc
    28801 ttagtatggg gattgcttat ggctcaagaa ccctcgatga aatcggtgga gaagctcaga
    28861 ataatttaga tgttgcttta gtccgtggag gcgaccaagt tgtcgtgaaa gaggtaaaag
    28921 aaggagcaaa acctgtttat tatgggggga aatctgctaa gaatgtcaaa cgcacgcgtg
    28981 ttcgttctcg agcgatgagt accgctttgc gtcggatttt tgatgaaaca ggcgatgtgt
    29041 ttatcatggg gcacaagttt ccggatatgg atgctttagg ctctgctttt ggtgtcagtt
    29101 gtctggcacg cttcaaccag aaaaaagctt acatcgttat caatgagaac gagatcattc
    29161 cagatgtaga acggtgtctt gaagaaatcc ataagcatcc tgaattagaa atgcaactaa
    29221 tctctccaga acgtgccata gagctgaaaa aagaagagga cctcctcgtg atggtggatt
    29281 accataaacc ttctctatcg atctctcagg gcctatatga gcaatttgat aagattgtga
    29341 tcattgatca ccatcgtcgt ggggaagagt ttccaagtaa acctctgctg acttatattg
    29401 aatcttcagc ttcttcagct tcagagctag tttctgagct gatccaatac caaagcagca
    29461 aaacaaatcg cttagatcgt tttgaggcca cattattgct tgcagggatg gttgtagata
    29521 caaatagttt caatgtccgt acaactgcac gaacttttga cgttgccagc tatttaaggt
    29581 cttgtggagc agacgcatcc ttagtacaat atttattgag ctcggattta gcgacatact
    29641 tggagatcag caatttgatt gctggaagcg actatgtgac ttctgatatt gttgtggcgg
    29701 cagggacaga cgaaaaagag tataatagtg ttatagcagc aaaaacagca gatacattgc
    29761 tttcgatgat caatatcaat gcagctttcg tcattacaaa gcggacagac ggcttgattg
    29821 ggatcagtgc cagaagtacc ggcacgatca acgtccaaat catcatggaa tccttaggtg
    29881 gcggaggaca cttcacaaac gcagctactc aactggaaaa tacgacagtt gaggaaacaa
    29941 aacagaagct atttgctgca atcaaagaaa atatgaatca aatatatgat aaggagtaat
    30001 ccaaatgaaa gtaatttttt tacaagatgt aaaaggaaaa ggtaaaaaag gggacatcaa
    30061 agaagttcca gcaggttatg cacaaaatta tctaatcaaa aataacttgg caaaagaagc
    30121 aacgaaaagc agtatcagtc agttagcagg acaaaagaaa gcacaagaaa aacacgaagc
    30181 tgaaatcttg gaagaagcta aaaagttaaa agaattcttg gaaaaagaag atactgtagt
    30241 cgaattgaag gcgaaagcag gggaagacag ccgtttgttt ggttcgatcc cttcaaaaca
    30301 aattgctgaa gggttaaaca aacaatacaa aatcaaatta gataaacgaa aaatcgaatt
    30361 agaaaatccg atccgttcac taggctacac aaacgtacct gttaagcttc atcatgaggt
    30421 gacggctacg atcaaagttc atgtcgtaca agaataacaa atcttgatag cgatgttcag
    30481 ctggtaagta taaccagaag gaaaaaagac aaaccgaaat ttatctgaga aaacagctgc
    30541 agcaatgaaa gcaaaagctg caaatcgatt tcagattgat ttcggtttct tttttgggaa
    30601 cagcaaaata gcataaagaa aacgagtata ataaaagaga actatttttt tattacactt
    30661 tcggtacgaa tgatgacttt tctctcattt tcttgtaaaa tgaagcaatt gaagacaagg
    30721 agcgatacgt atgaatgaag tgtggcagga tcgaatccca ccgcaaaata ttgaagctga
    30781 acaagcggtt ttaggctctg tttttcttga cgcagaaacg atcattgatg ccatggagta
    30841 catagagcca aaagactttt atcgtcgtgg ccatcaaatt atttttgaga caatgatcga
    30901 gttaaatgat cgtaatgaag cgattgacgt ggttactgtc aaagaccggc tggaacaagc
    30961 caatttgtta gaagatgccg gtggtttaag ttatctctca gacttggcat tagcagttcc
    31021 gacagcagca aatatcgtct actatgcgaa aatcgttgaa gaaaaatcat tgctgcgtac
    31081 attgatccag acagcaacag gaatcgttac gaaaggattc gagcaaggag aagaagttca
    31141 aacaatttta gatgatgccg aacgcagtat cttggaggtt tcggaaaaaa gaaatcgaag
    31201 cggcttttta tctattgctg aagtactgaa ctccacgatt gaaaatatcg atcgactagc
    31261 tcaaaacaat gaagaaatca caggtctacc aactggctat caaggtctgg ataaaatgac
    31321 agcaggtttc cagcccgaag aactaatcat tcttgctgca cgtccagcgg taggtaaaac
    31381 agcgtttgca ttgaatattg ctcaaaatgt aggaacaaaa acagatcgtt ctgttgcgat
    31441 atttagtttg gaaatggggg cagaatctct tgtaaatcgg atgctttgtg cagaaggttc
    31501 gattgaggcc agccatttac gtacaggtca gctttcggaa gaagaatggt cgaatctgat
    31561 cgttgcgatg gggagtcttt ctcgagcaaa tatttttatc gatgatacac cagggatcaa
    31621 aatctctgaa atccgtgcga aatgtcggaa actagcgcaa gaaaaaggca atctaggctt
    31681 gatattgatc gattatttgc agttgattga aggtaacggt aaagagaacc gtcaacaaga
    31741 agtatcagat atctctcgcc aactgaaaaa actggcgaaa gagctgaaag tacctgtcat
    31801 tgctctttca cagttatctc gtggagtaga acagcgacaa gacaaacgac cagtgctaag
    31861 tgatattcgt gaatccggat caattgaaca agatgcggac atcgtagcct tcttgtatcg
    31921 agatgattat tatgaaagag aaggcggaga tgacgatgat ggaaacgagc caaaaaatga
    31981 caacgtcatc gaagtcatca tcgagaagaa ccgaagcggt gcacgaggaa cagttgaact
    32041 actgtttatc aaagaataca ataagttttc gtcgctttcg ccaagagaag aattttaaag
    32101 aagaggttgt gagccttgag caatcccgag agctctcgta agaagatgca aggagtaacc
    32161 gcaggaacga ctagagtact taatttaaac agatctccaa agggattgtg acaaaagtct
    32221 tgtcacagtc cctttttccg aataaacggt gttcaatcag ctgacccttg gtattagctc
    32281 agcaaacgtc aaaatatgag gaactatttt gacgtttgtt tctctaatac tcaggtcaaa
    32341 acgctgatct tacgacctct tttccttaaa cggtgttctc ctgccaactc ctcggtcaca
    32401 agtcacaata tcatcaaaca tgagaatcag ttttttgaaa ttgctccttg tgactcagag
    32461 ttaaacggca ggctcacaac ctctttctta aacggtgttc aaaagcttgc atctgcggta
    32521 atacgcccaa tcaaacgaaa aatatgagaa gctatttttg tttggctgtt cttattactt
    32581 gatgcaggac agctttttca caacctctga ttcacggtgt tccaaaagta gcttcttcga
    32641 aaaataagtc gaaatttcac aaaaatttga gaagcaattt tcgtacattc cttcttattt
    32701 cttgaagctg accacttctg ttacaacctc ttttttaata accattctaa aaagataaag
    32761 ttcgtttttt aagcgttttt ataaaataaa aataaaaatc attcgatttt atttgaaata
    32821 tgtatctatt attgatacaa tggttcagga ttaaataata tagaacgagg tgttcacatg
    32881 tcatctgtag tagtagtagg tacgcaatgg ggcgatgaag ggaaaggaaa aatcacagac
    32941 tttctcagtg aaaatgctga agtgattgca cggtaccaag gtggcgacaa tgcagggcac
    33001 acaattaagt ttaacggtgt gacttataaa ctgcatttga ttccttctgg gattttttat
    33061 aaagacaaga tcagtgtgat cggaaacggc gttgtagtca atcctaaatc attggttaaa
    33121 gaattagctt atttacatga aaaaggtgta gaaacaagta atttgcgtat ttctgaccgc
    33181 gcgcacgtaa tcttgcctta tcatatcaaa ttggaccaat tacaagaaga tgctaagggc
    33241 gaaaataaga ttggtacaac gattaaagga atcggacctg cctatatgga taaagcagct
    33301 cgtgtaggta tccgtattgc agacttatta gataaagaaa tttttgaaga acgtcttcgt
    33361 ttgaatctag atgacaaaaa ccgtcaattt gtaaaaatgt ttgattcaga accacttgat
    33421 tttgacgata tttttgaaga atattatgaa tacggacaac aaatcaaaca atacgtcaca
    33481 gatacttccg ttatcttgaa tgatgctttg gatgcaggca aacgcgtgtt gtttgaaggc
    33541 gcacaaggtg taatgctgga tatcgaccag ggaacttatc cttttgttac ttcttctaac
    33601 ccagtagccg gtggggtaac aatcggaagc ggtgtagggc cttcaaaaat caataaagtt
    33661 gtcggcgtct gcaaagccta tacgtcacgt gtcggtgatg gaccatttcc aactgaatta
    33721 tttgatgaaa caggagaaca aatccgcgaa gtaggacgag aatacggtac gacaactggt
    33781 cgtccgcgtc gtgtaggctg gtttgattct gtagttatgc gccattctaa acgagtttca
    33841 ggtattacca acctatcttt aaactcgatc gatgtattaa gtggattgga aacagtaaaa
    33901 atttgtacgg cttatgaatt agatggcgaa ctaatctacc actatccagc aagcttgaaa
    33961 gagttgaacc gctgcaaacc aatctatgaa gaattgccag gctggtcaga agacattaca
    34021 ggatgcaaaa cacttgcaga actgccagaa aacgcacgta actatgtacg tcgtatctca
    34081 gagcttgttg gtgtacgtat ctcaacattt tctgtcggac ctgatagaac acaaacaaac
    34141 atcttagaaa gcgtctgggc gcaaatctaa ggataaaaac gacgactgaa tcaggacgtt
    34201 cttaccagag cgagtcgaag tgtttatcta tacgataata ataacttgaa gaggtagtga
    34261 aagagacgtt tagccttatt gccgataggc tgtctcttga acactgttta ttcagaataa
    34321 gggactgtga caaaaaccgt gtcacagtcc ctttttccga acaaacggtg ttcaaaagct
    34381 tgcatctacg gcactaagcc caatcagatg aaaaatatgg agtgctattt ttatctgatt
    34441 gttcttactg cttgatgcag agcagctttt tcacaacctc tccactaaac ggtgttctcc
    34501 tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ttttttgaaa
    34561 ttgttccttg tgactcggag ctaaacggca ggctcacaac ctcttggtaa acggtgttca
    34621 atcagctgac cctcggtatt agctcaaaaa acggcaaaat atgaggaact attttttccg
    34681 tttttttctc taatactcag gtcaaaacgc tgatttcaca acctctgatt cacggtgttc
    34741 cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg
    34801 taaattcctt cttatttctt gaagctgacc acttctgtca caacctctta ttttgattag
    34861 aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct ataatagacg
    34921 ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt aacagattct
    34981 tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt atcaatgaat
    35041 ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa ttatgaccgc
    35101 tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa tgttggccga
    35161 tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata tgtggctttt
    35221 tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt gctgaaggaa
    35281 gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat tggggaaggg
    35341 cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga agaaacccta
    35401 gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga cgatctgaaa
    35461 tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag tctattgagt
    35521 gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga caaagttcga
    35581 ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat cgttgcacca
    35641 cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga gaaggcaaaa
    35701 cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt aggaccaacg
    35761 atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat cagtcgataa
    35821 aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa cagcgactct
    35881 taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt tttaagctaa
    35941 ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt ttccaaaaat
    36001 tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc aatgatgcgg
    36061 tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga gccagtaaat
    36121 gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt cgttggatca
    36181 tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca acggactttc
    36241 tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa caacattctt
    36301 cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata ttgacgcaac
    36361 cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg taaataagaa
    36421 ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc acccatgata
    36481 actttgaata gcgtcgttgt tgttatatca ttttctgcga cgaaagcgac cttgtcttct
    36541 ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt tacacttaca
    36601 ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc aacgaatgga
    36661 taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat ttttttacgt
    36721 gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa ttcttggagt
    36781 tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc cgctagttga
    36841 ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc aaaatctaga
    36901 tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac aacgatcaca
    36961 gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat atccagaccg
    37021 tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg tgctaataac
    37081 acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa ttcttcaggg
    37141 atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc attaagctca
    37201 gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa atcctctttc
    37261 atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc catgatgact
    37321 gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac ggccatccgt
    37381 tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga taacacttta
    37441 aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt tccgggggta
    37501 aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact cacatcattt
    37561 acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt cttatagaat
    37621 tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt tcacgtgaaa
    37681 cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg atttattagt
    37741 ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa ctgtaaaaga
    37801 aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct cattgatttt
    37861 gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg gaaatttagt
    37921 tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg aactgtttgt
    37981 acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa taatcgggat
    38041 catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag tcgcagcatt
    38101 gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat ttactgctga
    38161 cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca atattttagg
    38221 aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga cattaagctt
    38281 tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg catttagata
    38341 agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt tgtaattgga
    38401 agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt cgagacaaat
    38461 ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg aagcatagcg
    38521 cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat ggacttcata
    38581 tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta aatgcagatc
    38641 ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag gacgatctga
    38701 ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg taaagctatt
    38761 tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata caccaaattt
    38821 cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat tcgaaaagac
    38881 aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca tgaccatagc
    38941 aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta gttgtgcggc
    39001 aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg tatgtttttg
    39061 tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga catctgtgac
    39121 gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct ctggacgaat
    39181 cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt ctttattttc
    39241 ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga caagtctttg
    39301 caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc gtccaaattt
    39361 tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg cacgtccaaa
    39421 tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt catttccggg
    39481 tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag gaataattcc
    39541 ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt ctttgatttg
    39601 cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg ctttttcaag
    39661 ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg ttttgtgagt
    39721 gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa cagggatagt
    39781 cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta ctacatgcgg
    39841 aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga aaataggatt
    39901 ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt gtcctaagaa
    39961 cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca aatgtgtcgc
    40021 accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg catctgcagc
    40081 ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt cggttttttc
    40141 ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt aaatatggtc
    40201 gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt ttgacatggt
    40261 gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta tagaactttt
    40321 tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt ttgggaatat
    40381 gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa tggctattga
    40441 gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt atgacagctc
    40501 ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta aacttgatgt
    40561 taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta gatccggcta
    40621 ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt gggtttggtt
    40681 taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat tttggaccac
    40741 aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt gttttttcta
    40801 ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcgat gcagcagcaa
    40861 ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta ggtattggta
    40921 tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa ggtctgagtc
    40981 gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt gctggagtga
    41041 ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt gcttttgccg
    41101 caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag cagacattgt
    41161 ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt atgatgattt
    41221 tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata gggccgcgaa
    41281 cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa tggtatcata
    41341 cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag ccagatgata
    41401 agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag gacactgaca
    41461 aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa gatatttaag
    41521 aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact atagaaaaag
    41581 tataacaggg attgtttaaa aacacctcca actgaagact gatttttact tcatctactt
    41641 aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg gaaaaggagg
    41701 agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc gcgattatca
    41761 tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag gcgctcgctg
    41821 cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg atttattgga
    41881 acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc ttatgggcga
    41941 acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg atcagtgagt
    42001 acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc gctaatattg
    42061 cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag aaagcattga
    42121 aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat gtgttaggaa
    42181 ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc gtgattctag
    42241 ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat gatccgtgtt
    42301 taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc gagtgatact
    42361 agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact gattactagt
    42421 tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt agttggtgtc
    42481 gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg taatctgatg
    42541 ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa acgtgttttt
    42601 atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt agtttttcca
    42661 cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaagg
//
LOCUS       DACESX010000007        42686 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.007, whole genome shotgun sequence.
ACCESSION   DACESX010000007 DACESX010000000
VERSION     DACESX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.007"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            473..799
                     /locus_tag="IXF88_000334"
     CDS             473..799
                     /locus_tag="IXF88_000334"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1507095.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            807..1715
                     /locus_tag="IXF88_000335"
     CDS             807..1715
                     /locus_tag="IXF88_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1507096.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            2344..2595
                     /locus_tag="IXF88_000336"
     CDS             2344..2595
                     /locus_tag="IXF88_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAQ1507097.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3137..3295
                     /locus_tag="IXF88_000337"
     CDS             3137..3295
                     /locus_tag="IXF88_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1507098.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            3367..4593
                     /locus_tag="IXF88_000338"
     CDS             3367..4593
                     /locus_tag="IXF88_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1507099.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(4682..5074)
                     /gene="rpsI"
                     /locus_tag="IXF88_000339"
     CDS             complement(4682..5074)
                     /gene="rpsI"
                     /locus_tag="IXF88_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1507100.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5090..5533)
                     /gene="rplM"
                     /locus_tag="IXF88_000340"
     CDS             complement(5090..5533)
                     /gene="rplM"
                     /locus_tag="IXF88_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1507101.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6052..6519)
                     /locus_tag="IXF88_000341"
     CDS             complement(6052..6519)
                     /locus_tag="IXF88_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1507102.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(6639..8177)
                     /locus_tag="IXF88_000342"
     CDS             complement(6639..8177)
                     /locus_tag="IXF88_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1507103.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLSGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            8257..8943
                     /locus_tag="IXF88_000343"
     CDS             8257..8943
                     /locus_tag="IXF88_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1507104.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9144..9671)
                     /gene="msrA"
                     /locus_tag="IXF88_000344"
     CDS             complement(9144..9671)
                     /gene="msrA"
                     /locus_tag="IXF88_000344"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1507105.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(9755..10432)
                     /gene="pgmB"
                     /locus_tag="IXF88_000345"
     CDS             complement(9755..10432)
                     /gene="pgmB"
                     /locus_tag="IXF88_000345"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1507106.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(10429..11559)
                     /locus_tag="IXF88_000346"
     CDS             complement(10429..11559)
                     /locus_tag="IXF88_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1507107.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(11559..13742)
                     /locus_tag="IXF88_000347"
     CDS             complement(11559..13742)
                     /locus_tag="IXF88_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1507108.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(13739..14764)
                     /locus_tag="IXF88_000348"
     CDS             complement(13739..14764)
                     /locus_tag="IXF88_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1507109.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(14809..15636)
                     /locus_tag="IXF88_000349"
     CDS             complement(14809..15636)
                     /locus_tag="IXF88_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1507110.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(15633..16700)
                     /locus_tag="IXF88_000350"
     CDS             complement(15633..16700)
                     /locus_tag="IXF88_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1507111.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(16726..17562)
                     /locus_tag="IXF88_000351"
     CDS             complement(16726..17562)
                     /locus_tag="IXF88_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1507112.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(17559..18428)
                     /locus_tag="IXF88_000352"
     CDS             complement(17559..18428)
                     /locus_tag="IXF88_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1507113.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(18498..19781)
                     /locus_tag="IXF88_000353"
     CDS             complement(18498..19781)
                     /locus_tag="IXF88_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1507114.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(19795..21480)
                     /locus_tag="IXF88_000354"
     CDS             complement(19795..21480)
                     /locus_tag="IXF88_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1507115.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            21755..22780
                     /locus_tag="IXF88_000355"
     CDS             21755..22780
                     /locus_tag="IXF88_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1507116.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(23375..27028)
                     /gene="rpoC"
                     /locus_tag="IXF88_000356"
     CDS             complement(23375..27028)
                     /gene="rpoC"
                     /locus_tag="IXF88_000356"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1507117.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(27107..30724)
                     /gene="rpoB"
                     /locus_tag="IXF88_000357"
     CDS             complement(27107..30724)
                     /gene="rpoB"
                     /locus_tag="IXF88_000357"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1507118.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            31452..32420
                     /locus_tag="IXF88_000358"
     CDS             31452..32420
                     /locus_tag="IXF88_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1507119.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            32438..33343
                     /locus_tag="IXF88_000359"
     CDS             32438..33343
                     /locus_tag="IXF88_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1507120.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            33340..34146
                     /locus_tag="IXF88_000360"
     CDS             33340..34146
                     /locus_tag="IXF88_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1507121.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            34303..35271
                     /locus_tag="IXF88_000361"
     CDS             34303..35271
                     /locus_tag="IXF88_000361"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1507122.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(35588..36112)
                     /locus_tag="IXF88_000362"
     CDS             complement(35588..36112)
                     /locus_tag="IXF88_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1507123.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            36399..38113
                     /gene="scm"
                     /locus_tag="IXF88_000363"
                     /pseudo
     CDS             36399..38113
                     /gene="scm"
                     /locus_tag="IXF88_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
     gene            complement(38268..39254)
                     /locus_tag="IXF88_000364"
     CDS             complement(38268..39254)
                     /locus_tag="IXF88_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1507124.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(39277..39843)
                     /locus_tag="IXF88_000365"
     CDS             complement(39277..39843)
                     /locus_tag="IXF88_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1507125.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(39857..40273)
                     /locus_tag="IXF88_000366"
     CDS             complement(39857..40273)
                     /locus_tag="IXF88_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1507126.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(40730..42664)
                     /locus_tag="IXF88_000367"
     CDS             complement(40730..42664)
                     /locus_tag="IXF88_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1507127.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
BASE COUNT        12295 a         9091 c         7977 g        13323 t
ORIGIN      
        1 aaataaactg taaaaactta tatttctatg agtggtggaa caatgctaat accaaattaa
       61 tttatcgatt acccacctgc acaaaccaaa gatagcatta ttaggaaacg aatcaatcag
      121 cgaatccagc attataatga ggatactaaa aataaagttt tcataattat aaatccgcct
      181 tcctacgaaa ggcaccaatg aggtgatgaa aaccttagag cgctttcttt ctttttttat
      241 tccgtgttac cctttccttg caggtccctg gtacactaca gaacccgagg aggtgagtgg
      301 cggggctgta tagcggcaac cccgcataat tatatggtgt cggtcatccg ttaaggcatc
      361 aatgaatggc gcaaacccgt agcccgtaaa cttatctctt ccgaagttga ctcgatttcg
      421 ccggatgacc agtccctacc aaacctgtac tattttttta caggaggttg ctatgttcaa
      481 aatctttcgt aaaaattgtt gtggcttgga tgtccacaaa acgtggatct atgcgtgtat
      541 cggaatcaca gatgcgaatg gtcgtacagt gtataagcaa gctcgcttct cttcctttac
      601 aaaaggtctc aatgaattag ctgaatggct tgctaaatac aactgtaagg atgtgtgttt
      661 ggaatcttcc ggtaaatttt ggattcctgt ttttaatatt cttgaaaaaa cttgttttgt
      721 cacactggct catccgaagt acaccaaacc ccagaaagga aataaaacag atcgcaaaga
      781 tgcaaaaatg gatttgtgat ctctttatgt gcgacatgat caaacccagt tttattcctg
      841 gtcttgaaat ccgccagctt cgtgatctaa tgcgttatcg gacgaaactg accaacatga
      901 aaacaagtga aaagaatcgt gctttaaact gcttaaccgt ttctaatctg aaactagatg
      961 atgtttttag tgatgtgttc ggaaaatcat ccacttctat catccaatac ttgttagaac
     1021 atcccggtga atcatttgat gttcgaccat ttatccatgc ccgttgtaaa actcccgttg
     1081 aagaaatcca agcggcagtt gagggggcga tttctgtacc tcaagcgatt aagcttcgcc
     1141 aatgcctcaa ccatattaac gagattgaga agcatcgaga agagatcaat ctagagattc
     1201 aagctcttgc agaacccttc tcccctgttt tggaactact tcgtaccata ccgggcttag
     1261 acactcagcc aataacagcg attgcgattc tttcagaaat aggaccggat atgtccgtgt
     1321 ttccaacttc gaaacacttt atttcttggg ctggatgctg tccaagaaac gatcgcagtg
     1381 cccgacgaac aaaatcaaca cgtatctctc gtgcagggca gtcgttgaaa ccgctacttg
     1441 tacagattgc caatgctctt ctcagatcca aagaacatcc agaatttaaa aatcgctatc
     1501 gtagaatcaa agcgaatcgt ggacacaaga aagctatcat tgctatttgt aagatgctcc
     1561 tgaccgctat ctggaatgtc ttatcaaagg tggaacccta ttctgctaaa ggatatctgg
     1621 agccaaaacc tgcgaaagaa gagaaagttc tgacgcaatc ccaagctctg gaccttttaa
     1681 gaaaacgcgg atataccatt atagatagtg cttaaaccaa agttacgttg attcttccta
     1741 aatagactcg ttgtcttcca tttcaaaaat tttgaaaacg gtaggcttat ttagtgtacc
     1801 gttcttcagg tatcttcaaa tagatttatt ttcaaactta tttcctttct tcttttactc
     1861 tcctatagct tagcatttaa gtgggcactc gtgtcacctt gctagatagc tatgaatgcc
     1921 gtaataagcc tcctagtata taggcagact gccaattata actgattcaa tccacattat
     1981 gataaaatcc accttttcgt ttcatatgtg ttgaaatatc acatatgtca aaagggcacg
     2041 agtcttttca ttcagtgctt tgtaaatact caaaattatt ccttttcact ttaaaaaaaa
     2101 gataattagt acaaaatcag aaatgaggaa agtattagga tggactagaa aggagttgtt
     2161 gaatttgttg caacagattg gtcatttatc aattaaaaat taggtgaagt tttattcatc
     2221 actgcagctg ctgtttccca gagaataaaa cgtctagaag aaactggtca gttactgtat
     2281 tatcaagcag caattgacta cgaacaagct tgattactaa ccaaagcgtt tatccacctg
     2341 ttgattaaat caaatcaaaa atcaggtttt tatacgttca taaaagcaat ctccagtgta
     2401 ctcgaatgtg actgtatcat aggtccttat tcgatgttaa tcaaaatggg gtttccttct
     2461 accgaatcat tggacagttt tatcaatgat ttatcaaagt ttggacgaac caacacccaa
     2521 atcgttttct cgactcttgt ttccttaatg gtttatcagt tctggaatgt ttatcatgcg
     2581 cttttattaa aatagaaaag agcttttttc atgcgacaaa gcagtgatca tttattggca
     2641 catgcaatct ttgtggatcc attttttgaa aatacgatat ttagttttga cccaaaaatc
     2701 tttaaatacg tatttgataa tttatcttaa caaccagata attttattca gcaccgtatt
     2761 atctatattg aattctcgga atcaagataa atttttttcc gtttttgttt cttttttaat
     2821 cttcttcttg ttattaatag tgattaggat gatattccta tattgatctt tgataattag
     2881 atataatttt tgaataacgt tccctgctat tcgagctaga aatacaagta cgccatgacc
     2941 tagttagcga caactactta gagatttctt ttgttctggg acaacaaaat gccatgacaa
     3001 tagcagtaaa taatgatact tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc
     3061 tatgtgctta cccaaagcgg ttcaaaaatt ttaaactatt attttaggag atgattttat
     3121 aaaatcgaaa gcctacttga ataaaaaaca agcctgtgaa tttctaggaa ttgataataa
     3181 tactttagat aagtggattc gtgtatataa ttttccagta ataaaaattg atggcgtagt
     3241 tcgctttgac gtagaggata tcaaagattt catgaataca ttcaaaaaaa attgaaattt
     3301 tcttctcgtc acatatacta taaatagcga aatgtactgc cttacttttt aaaggagtga
     3361 ggaagtttgc ctacaaagct ttcaaatggg aaatataaaa ctaatctgag gtatccaaaa
     3421 aaattcagag aaatcactgg aattgcatca gagaagtatc aaaaagtatt tccaactagg
     3481 caattagcta ttaaggccga aagggaaatg aaaaagaaga ttgaaacagt tttaagagag
     3541 gagaatgcaa actcactaga attgaaagga aaaataaaat ttaaagattt ttatgaatca
     3601 aaatggcttc ctagatatga actaggtcaa actactaggt caaacagggc tccaagtaat
     3661 gtaacaatta gtaatactaa ggatatattc cgattgcaca tccttccaat gtttggtgaa
     3721 tatgcaatga attatctgaa ttcaaatact gagataatat cagatgaatt aacaaagaaa
     3781 tcgaaagaat acgcaaatat aaaaattatc aaaggctatg ttcgctctat gtttgatatt
     3841 gccgaaatac tatgttatat cgaattcaac cgaactacca aaattattca atgtattacc
     3901 gcgcctaaga aaatggcttt agaagaaaaa aggataaggg aagggaatca agcattatca
     3961 tcagaagagc tgactgattg gatagaagca gtaaacaatg acttaaataa tcacttgctt
     4021 acacttcacg attacacact tttcatgctt accttatatc taggtgatag aaaaagtgag
     4081 acttatgctc tgcaatggaa atatattgat tttaataaaa atacggttcg tctcaaatat
     4141 gctttagata aatatcaaag aaagacacat acaaaaggtt ggaaagacac cgttattcag
     4201 gcaccagaag ttgtgatgac attattaaga gaatggaaat cagctcaggc tgaacaattg
     4261 ttgaaactaa aaattataca aacacctgac cagtacctct ttacctattc caaaccttct
     4321 ggagaagtaa attgtcctgt tcacgctgac tacttaaact atcgaatcaa tgcaataaaa
     4381 aaaaggcatc ctgaattggc tcaattatct ccacacaaac ttcgtcatac ctatgcaact
     4441 atagctaggg aaggtggagc aaatatgaat caaatctcta atgctttaac gcactcggat
     4501 atttctacta caaaaatata cgtgaacaca cctgacgtag ttgataaatc tgtttttgaa
     4561 gcatttcaaa aaggattgaa taagtgtgat taacatgtgt tctcaaatgt gttcttatta
     4621 taaaaaaaga cactttccaa ataattgaaa agtgtcctga aatattgata taaaaagaat
     4681 attaacgttt tgagaattgt gaagctttac gagctttttt aagacctggt tttttacgtt
     4741 cgaccatacg tgcgtcacga gtaagtaagc cagcacgttt aagagctaaa cggaagtcag
     4801 gatcaacttc tagcaatgca cgagcgatac catgacggat agctcctgat tgtccagcgt
     4861 atcctccgcc gtttacgtta acaaatacgt catatgcgcc ttttgtttca gtaacagcga
     4921 aaggttgatt gattacttca cgcaagtcag catgtggaat gtattcttca acatcttttt
     4981 tatttacagt aattttgcca gttcctggta ctagacgtac gcgagcaact gcgtttttac
     5041 gacggcctgt gccgatatat tgaacttgtg ccaatgaaat tccctcctat tagattaagt
     5101 ttgtgatgtc taaaacttct ggttgttgtg ctgcatgtgg atgttctgct ccaccgtata
     5161 cgttcaattt tgtaaattgt ttgcggccta aagtgttttt aggaagcata ccttttacag
     5221 aagtttcgat caaacgacga gaatttttag cacgcaattc gccagcagtg attgatttca
     5281 atcctcctgg gtagttgctg tgacggtaat aaattttgtc agtcgctttt ttacctgtta
     5341 atttcacttt gtctgcattg atgacgatga cgaaatcgcc agtatccaaa tgaggtgtga
     5401 aagttggttt atttttacca cgtagtacag atgcaacaac tgttgaaaga cgtcccaatg
     5461 gaacatcagt tgcgtctact acataccatt tacgttctac ttcgcctggt ttggccatat
     5521 atgttgtacg cacgttattt tcctccaatt taagttctga tgtttgacat acacttcgag
     5581 tttccggggc tctttgtggg gcaaacaata ccatttaaca tattacccgc taatctagtc
     5641 aatgtcaacg tttttctgct tgattgaaaa atttatatga aactttactt cttttctttc
     5701 ttttatataa aagggtcgtt tcctgtcaaa atgaaaaaca aaaagaggct gtgacaaaag
     5761 tcttgtcaca accccttatt ttgaataaac ggtgttctcc tgccaactcc tcggtcacaa
     5821 gtcacaatat ccataaaaca taagaagctg ttttttaaaa ttgtttcttg tgactcggag
     5881 ctgaacggca ggcttacaac ctcttggtaa acggtgttca atcagctgac cctcggtatt
     5941 agctcaaaaa acggcaaaat atgaggaact attttgacgt ttgtttctct aatactcagg
     6001 tcaaaacgct gatttcacaa cctctttttc ggtcaagttc aacaacagtt tttacgcgtc
     6061 gatctctggc gctttgccca aatgagcttg gatcatccag atacgttttt ctgtggctgt
     6121 tttgaattcg ataaagatat cttgtgttgg atcgtcgcct tcttctccag aaacttcgat
     6181 tcctttttgg taaagatctg ctagataacg atagcctgca acaagtgttt ccaaacgttc
     6241 tggaatagta cgatcccagc ggccaatttc atcagggatt tttgtgttat ctgcaaattc
     6301 acgcaatgta gaataaggtg aaccgtctaa tgtgatcaag cgttcggata cttcatctaa
     6361 ctgagcgttc aaatcatcca tgaactcatc catcattggg tgcaatgtaa ggaaacctgg
     6421 tcctctcatg taccaatgtg tttggtgaac aacaacagac atttggctta gatctgcaac
     6481 taattgattt aatacttctt tggtttgttg gaatttcatc tttcttgcct cctaacattt
     6541 tcttacactt aaaataatag tagactttca atgaaaagta caacaaaatc atttacctga
     6601 gaacgatttt cagttcgctt ttttatccgt aaagccaatc atttttcaga atcagctaat
     6661 tcttctcgca gttgagcaaa ttgctgataa gtctgtgcta aatgcttttg tgtcgaaaca
     6721 aataatggat agattttctg atatttttta tgatcagttt cttgtactcc aacttgctct
     6781 ttcaattcta tcaattcctg tgcttcactg atactttgga tctttccgat gcttctccag
     6841 cctaaaatca cagctcctaa tgcggatgct tctgtgacag tagggaaaac aagcgggtaa
     6901 cccagtacat cagccatcat ctgacgaaaa atcggagatt gagcaaaccc gcctgtagca
     6961 cggatctctg tgagctgatt ttcctggcaa actcctttta aaatccgata aaggttgaga
     7021 cagatccctt ctagaatcgc cttcatcatt tctttttctg tatgttgccg ctgtaaaccg
     7081 ataaaactgc caaacgcatc tgcgtcccaa agcggtgctc gctcaccaag taaataggga
     7141 tgaaaaatca gaccattggc acccgctggc acaaaagcga tagccgaaag cagtgcatca
     7201 tatggatttt ctcctttttc tttagcagat tgaacgactt cctgtaaaaa tgtatgccct
     7261 gcccaatcaa agatgccagc accattgcta gtcgctcctc ccactaccca atgtttctga
     7321 tccagaacat aacaaaacgt ctccatttct ggatggaact gaggctgagt agtaataaaa
     7381 cgaagtgctc cgctcgttcc tacggtcaaa gcggcgatcg tatcaccaaa agcacctaac
     7441 cctagattag aaagcggccc gtctgcccct cccaaaacaa atgatatatc atttggcaaa
     7501 cctagttctg ctgctgtttt tgatggtaga cctgttactt gataggtgga aggaaagact
     7561 tgcggcaatt gctctattcg aatgttcaag tagtcaagca cttcttctgc ccattggaat
     7621 tcatggatat caaaatatcc agttcctgaa gctgttccgt agtcgctgat cagttcgcca
     7681 gacaatctat aaaagatata gtctttgata ccgacaaaat aagcagtttt tttgaataat
     7741 tcaggttggc tgttttgcag ccacttgatt ttgttcaatg gtgtcattgg atgaatcggc
     7801 gtcccagtct gccgataaaa ttctttgcca agcacggtat atttcagttt ttctgcttcg
     7861 tccgctgctc ggttatctgc ccaagtgatc attcgagata acggtgtttt ctcttcatcc
     7921 aacaatatca ggccatgcat cgcagaggag aaggagacaa attgaatgat tgtgtctgaa
     7981 ggcaactgtt taattacttc ttttataacg gtgcagacac cttgaaagat caattcctga
     8041 tcttgttctg ccatcccttg tgtttcttgg atcaaagggt agcgaaaata agcggatgct
     8101 gcttcttggg cttcatctgt gaaagcaact gctttggttt gtgtggttcc gatatctaat
     8161 ccgattgaaa cgttcatttt ttcacctcaa attttatgta tgtacaaaaa tttagtttaa
     8221 gcttgcgtat cttggttttg aaaaggggga gatggcgtgt tactttattt tatcattggt
     8281 tgctgtatcg gatcattttt atgtttaacc gcccagcgga ttccgttagg tcactctatt
     8341 atttatccta gatctcattg tgtcaaatgc tgtcgttcgc tttcttggta tgaattgatt
     8401 ccgattctct cgatcatcgt ccaacgattt cgctgccgct attgcaggtg tcggttgccc
     8461 ttctactatt tgttggcaga agccttatgt ggtggtcttt ttgcctggtt ctttactttt
     8521 tcttccgcaa gaaatttttc gacgttcata tggatgcttt cggctttgct attttcgttg
     8581 atggatttat tttacttcat ggtccattct cacacgctat tctgttcttg ggcaatttta
     8641 tggatttttt ggttgcaggc cggcgtcttc caatggcaaa gtgtgctgtt aacgtttatc
     8701 gtaggcggtg tgtgccttcg ctatggtcaa tcctatctcg gagctggcga tactctgttg
     8761 atgctttctt ggagtggtgg cttgtctcta gaagagttgc ttcagatctt gtttcttgct
     8821 agtctgtttg gaatatccgt tttttctgtt tactttatct gtcataaaaa aaagttggaa
     8881 aagctgcctt tcattccttt tttgagcagt gcgttgctga ttgtccttca tcttaggtat
     8941 tgaaaaaagg ttgtgacaaa cgacttgtca caacctctga tatatagtgt tctcttacca
     9001 aatccttgtg acttggagct aggcgacagg ttcacaacct ctccactaaa tggtgttcaa
     9061 tcagctgacc ctcagtatta gctcaaaatg ctggttttac aacctctgat atacggtgtt
     9121 ccataagtag ctttttcggc ttcttatttc ttgacgcccc aatgtcgacg aaggaaacct
     9181 gcacgtccag aaccttctcg ataaagattg taatggagct tgtttttctt gtaataatcc
     9241 tgatggtaat cttctgcagg ataaaattcg cttcttggtt cgatttttgt cacgattggc
     9301 ttatcgaatc gcccgctgtc ttgcaatgct tgttttgaac gctctgcaat atttcgttct
     9361 tcttcatcat tatagtagat cactggacga taagaatcac cgcgatcagc gaactgtcct
     9421 aatgcgtcag tcggatcggt ttgctgccaa tagatttcca ccaactgatc ataagaaata
     9481 acttctggat catattcgat ctctactgct tctgtatgcc ctgttgtgcc tgttgttact
     9541 tgttcataag ttggatttgg tacatgcccg ccagtataac cggatgtcac agaaatgatc
     9601 cctggctgct cttcaaatgg tttgaccata caccaaaagc atccgcctgc gaaaactgct
     9661 ttttttgtca tataaaatcc cccttttttc tcatcatacc ttgatttcca aaaggaggat
     9721 aacgttttgc ttgtgacaga aaaaacggaa actattatat acgtgatacg gcttctgata
     9781 aatgccaaaa cgaacgtact tgatcaaaaa cggttaaatc aggaatcaca tattcagcag
     9841 aaggaagatt tatgttttca cctattccca atacatgcat gttaccagcc aaagctgctt
     9901 gacaaccagc ttctgaatct tcaatgacca agcatgcatt tggcggtaca ttcaattgct
     9961 gtgctccttt gagaaaaact tctggatcag gttttgcttt tgatacttgc gtgccgtcaa
    10021 taaggacatc aaaataactt gtcaaattcg ttttttctaa aatgaggcgt gcatttttgc
    10081 ttgctgatcc caatccgatc ttgatgtttg tttttttcaa gtactcaaga acagctattg
    10141 caccaggcaa aacggatgtt tcatccattt tttgaatcgc ttgaagatat agattgtttt
    10201 tttgctcagc aaaggctgat ttctcttttt ctgagaattt ctgttccatt tttccaaatg
    10261 acagcaatcg ctctagtgac tcggttctgg atattccttt cagttgctcg ttttctttct
    10321 cagtgaatgg aatggacagc tggttagcta aaaccagcca cgcttcgtaa tgataacgag
    10381 ctgtgtctac aagaacgcca tctaaatcga atagtgctgc tttgattttc atcgattacc
    10441 tccttgaaaa tgaacgactt gaagtaaaga tgcgatgttt ttagctgtgc gatgaagatt
    10501 atcagtggct tgctgtaaag cttcttgtaa aggcacagct gcaggaagga tcggaaaaac
    10561 ggccgaaatg ccaacatctg ctgctttttc gcttccttta ccaacacttc cgcaaatagc
    10621 tatcacagga acgccctctg gtactcttct agcaatccca actggcgctt ttccttttag
    10681 actttgtcca tctagacgtc cttcgcctac gattacgagg tctgcttgct gtacccgttt
    10741 atcgaactcc agtaaatcca atacgtagtc gatgccagag actattttcc ctgaagcaaa
    10801 tgctaacagt cctgcaccca ttccaccacc agcactactg cccgatgcct cgattacttg
    10861 aggggcaaat ctcatataaa aatcatgcat ggcttggtca gccactgcta attcattttc
    10921 tttcaagcct ttttgtggtc cgaaaacaaa agaagcgcct tcttttccgc ataaaagatt
    10981 agtaacatca gtaatgacag tgattttggc tttaatcagc tcaataggca cgttcgtttt
    11041 atcaactgta gcaattttac ctagattttc tcccagtgct tctacttttt gattatcttg
    11101 atcgtaaaaa gcataaccta gaccttctgc catgccgatg ccgccatcgt tggtactact
    11161 tccgccaacg ccgatgatga tgtgtcgaac accttgttgc aacaattgtc gtatcagttc
    11221 tcctactcct cgcgtggata acctaagtgg ctgttgttgg tttagtggga catctttcaa
    11281 tccgacgatt tctgccattt caaaaatcgc agtttgaccg tctgaagcgt actggatttc
    11341 tttgtttttg ccaaatgcat tagtaacgac tgctgtttgc cgcttcaacc ttaaactgtc
    11401 tgctaatgat gaaaccgttc cttctcctcc atcgcctata ggtagcagat catattcagc
    11461 ctgaacaaaa acttcttgaa aacctgattg aatggcttgt gctgcctcta aggctgtcag
    11521 actttctttg aaagaatctg gcgcaatcag aatacgcatt attttcccct ccgttccaag
    11581 atcagttctt tttccagctg gtattggtct ccattgacaa aaataggtaa tgtttctttc
    11641 gtctcttttt ttattactat ttgttttttt gtcaaaacaa atagcaacct gtgatttttc
    11701 caaataaaag gaaaagaaat tttttcccat gctgaaggaa gtcttggaga aatagaaaga
    11761 gttccattcg ttactgtaat acctgaaaaa ccaaatataa ctgctagcca tagtgctcct
    11821 aatgaagcag catgaattcc gtcatctgat gaatgcggat ctggtcctaa atcaatcagg
    11881 cacgcttctt ggaataaatg atacgcccat tccatttctt ggatacggca agcctctatg
    11941 gcgtgaatcg ctttactcaa cgatgagtcg tggattgttt tgttttcata gtaatgcaaa
    12001 ttcttagaaa cgattggttg agaaaaatac tcaggtaata agtataacag catgacaaga
    12061 tctgcttgct tgctgacttg gagttcattt acttcgctac gggcataatc aagtaaaata
    12121 gactggctgc caggacgttc tttgtaagtg gtcaaatcaa taactggctt actgagatac
    12181 gtgtcatctt gcggaatcag ctgttctttg ttaggcactg gcaaatataa cttatctaaa
    12241 aaattttgac attctttcaa aaaaagcgga tcgccagctt gatatttaac agctaaggcc
    12301 acattataat gcgccagata gttcgtatat gcattattgt cgatatattc tgtgtattca
    12361 tctggaccaa tgactctgtg tatctctaaa tgatcatcga cttttacagc tctgctcagc
    12421 caaaaagctg ctgtttcatg aatgagcgtt tttccctttg tttctaaaaa cgattgatcc
    12481 tctgttgctt ggacatactc ttgaacggca taagcaaggt ctgcactgat atgatgttct
    12541 gcggcagctg aagcaacttc ttgtcttgtt ccggttttga tattcaatgc agcaaatttg
    12601 ggtgtctctt cctgaccagt gtgtgcgctt tcccatggga aaagtgcacc ttgatagtga
    12661 taatctcttg ctttttgccg tgcgccttct aatcgatcat aacggtacag cagcaaattc
    12721 tttgctactt caggaaactg atataaaaag aaaggcagga taaaaatttc cgtatcccag
    12781 aaaacatgcc ctttataacc ttcgccagtt agtcccttcg cccctacact aacacttgga
    12841 tcattgcggg gagtcataat ctgtaaatgg taagcagcga agtccatcgc taactgatcg
    12901 aaagaagaat cgccttggat cgttatccga tgctgatgcc aaaaatctgt ccaataagac
    12961 atcgactgct ctaaaatttc tgtataacta acgcttttct gggcttgggc aatacttttc
    13021 ttaaacgggt ctgtttgctg aagagaggta gagagcacga tgatcttctc cagcgtaaag
    13081 gacgtatttt ttctgatctt ttctgaaaag gaagcggtga tccggcgatt ttttgcttgg
    13141 aatacacctg ctttattgaa tttagctgca atcccaatat caattttact ttctgttgtc
    13201 tggtaggatg cactgatcaa atcgtcacca aatactcgca gttctttttc aaccaactgc
    13261 tgtgtaccaa aattggtttg ctgtccatcg atacccgttt tcacatctac ttggcagtct
    13321 tcggtcaacg gcttgatgac cactttcagg gctgcctgat ggagattatt ttgatcagca
    13381 acacgacaag taatgacttc cacttttttt ccttgagcag tttcaatgag cgcatagcgt
    13441 ttaagctctc ccatttctaa atctagtgcc cgctcgtatg caagaacttt catcgtctca
    13501 agagaaaaat gttcaccgtc gatttttatt tcccatctga tgacatctgg gagattcact
    13561 aactcggctg attctgctcc taaaggtcgg ttatatacac cagctgcaaa cattcccctt
    13621 acttgattcc gatagtcttc ctcctggctt cctctgatac caaaaaatcc atttccaatg
    13681 gtcatctgac tcgcatactt attgatatac tctaaatcaa actgtttttc ttctactttc
    13741 ataaggtaat aacttctcct gtttctgccg aacgatataa attttccacc agattctgga
    13801 tgacatatcc ttgccagcca tctgcaatca tctgagtctg gtcaccgagc gcccgattca
    13861 caaaatgagc catacttagt tcatgtgggt cattaactgg cgtggtcttt tgtaaggaaa
    13921 ccagctttcc atctacatct tcgaatagtt cgcctggata cagattagca cctgcctgat
    13981 cgccacaaaa agccacattc agtttgtctt ctctgatatt caaagcaaac gaagtattca
    14041 gttggatgat cttgccgttt tctaattcca gtgtgccaaa aattgcatct tctactgtga
    14101 atttgcttgg atcccatttc ccgaatttcc ctgaattttt tttcgtacca attttttgga
    14161 agctgtacgc ccgaacgctt tttactttag gaaatccagt aatatacatc gctgtatcaa
    14221 gcatgtgaat gccataatcg atcaaaggcc cgcctccttg aagttctttg tcgataaacg
    14281 ttccccagcc tggaacacca gaccgacgaa gcgcattcgc ttctacgtaa taaatatccc
    14341 cgagaacatc aagatgattc ttgatcattg ttgcttcgtc agaaaaacga tgctggaatc
    14401 cataagccaa aatctgcccg tttttatcgg ctgtctctgc catctttctt gcttgatcag
    14461 ctgtcatagc tggtggtttc tcgcacatca ctgaagcatt agcttcgagg gcagccaata
    14521 catgttcgaa gtgaaagcga ttgggtgtac agacactcac gatatcggga gaaatctcat
    14581 tcagcatttg tgtaactgat gtatacgcat gagaaaatcc tgtacgtcgg caaaagtctt
    14641 ctgctcgctg gctgactgga tcaaccacag cggccaattc taagacatct gatctttttt
    14701 gataataagc agcatgtact ttttctgcta cttgtccagc gcctatgatt gctactttct
    14761 tcatttattt ctccctcctt tgcttattta cttattgtta ttctttctct attctttctc
    14821 taaatcattt aaatgacgcg ctgtttccag acagtttttc aagtattgca atgattcctg
    14881 ataaattttc acttcatttt ctcctcgcaa acgacattca tataccatat aaccttcata
    14941 ctcattttga agtagcgttt gaaaatggcg agtaaaatca attgcgccag atcctggttg
    15001 gtagcgatga ttatctgcta aatggatatg acccacatac tctttttgcg tagtcaatgc
    15061 ttttgaaata tcatcttctt caatattcat atgataaaaa tcagcgatga tctgcacatg
    15121 tttcaattga ttagagtcaa tgtaatgacg cgcatccgct aacaaattga tcatatggtc
    15181 ttggtatcgg ttcaaaggct ccaaataaat aaccgtacct gtttcttcgg ctactttatc
    15241 taaataacga agtgattcgc tgactgcttt atagtctcct tctttgcttc ggggtgaaac
    15301 catcggcggt aatcgataag tgaacattcc ccaagctgct gggataacga ttcctttgcc
    15361 tccaacctcg cttagtgcac gcaaaatctc agcaatttga cgtacaccat taagacgccg
    15421 ttcttcgata aagtcaccga tccacccatc gtagccgtta caagctgtca ctactgggat
    15481 ccctgtttct ttgattgcct gtttgactgt gtcgatattt tctattaaca acttaccgtc
    15541 gatttcgtat tcttcaaatc cacagtcttt gataaatcta aatttttcta aaatgtctct
    15601 tgggaaaaat gcttgatctt gcgtaccgaa tttcattgtt ctcctcctcg gacttgaacg
    15661 cccattttga tactttcttc tgggtgctgg tctacataac gcatgtaagc ttctgcgctt
    15721 tctgcaaatg gcacaactgg atcaatgatt tcttcacaat ccaagtaacc gttcatcaat
    15781 aattcccaac atgtgtcctc gattcgttta cggttccatc ttgggtaatc tgggtttggt
    15841 tcactagatg ctcgagcaaa gacgattttt gcattattga aatgtgcttc gcgaccaaga
    15901 ttaaacccag ctggaaaagg tttagcaaag gcaacataag aaatgatccc gccatagccg
    15961 atccctctta aagaagcttg taatgcggca gcactgccac tcgtttcaat aactgaatct
    16021 actccacgtt tatctgtcaa ttcctttaga cgaaaaccga catctgcttt cataggatct
    16081 agcgtgtaat cggcaccgta tttttctgct aattctctac gctgcgctat gggatcgatc
    16141 ccaacgatgg tagtagcacc tgctttttta gctaattgaa tcgcaatttg cccgatagcg
    16201 cctaaaccga agattgctac agcatcacct gctcgaacat gtgcttcccg gataccacct
    16261 aaagcgaatt gagcaggatc gtaacatact gcatttttcc attgatccaa ccgatcaagt
    16321 ttacgtagtt tataattatc aacaccgttt gtaatcagcg tttcacgaat gggtccataa
    16381 gtgcagacta actctccttt ttgatactct gtcaccaaac tgcccgtttc ttcgatcgta
    16441 ccgacaatca tatttcctaa ttgaaattcg ccaaatacca caccgcgttg gcttccttct
    16501 tctcttggca agaataggcg ccattcttct gaaaattctt catcgataaa tggacttaat
    16561 cctctaaagt cgacaatttc tgttcctggt tttggagtag cgaaaattac tcggatacgt
    16621 acttcgttct tttcgatctc acggtcttcg taatctttga atccggctat tcttggttct
    16681 aatgcaacta attttttcat cttttctttc ctctttcttt tctttttatc cttttacgcc
    16741 gccttcgact aaatcacctt taataaatcg ttcagataac gcatacataa tgatgactgg
    16801 taaagcggtc accaatgaag cggccatcat tcgtccccaa atgtagtctg gtgtactgaa
    16861 taatgtgttc aaaccgattg gtaaagtaaa tttttcggag gtcgacagga aaatcgaagc
    16921 aaataaataa tcattccacg aaaccataaa gcaataaacg aagacagaaa tgattccaga
    16981 aatagccaat ggtataatga tccgaataat gatctggatc cggctaagac catcgatcat
    17041 tgctgcttct tcaatatcag tcggaatcgt gtcaaaataa ctttttagca tgaatatcgc
    17101 agtaggaagt gtttgaacga ccatcgtgat aatcagtgct gtattggtat catacaaacc
    17161 tagaccggaa atgattttaa ataatggaac aacgatcaaa ataccggaaa acatataaac
    17221 ggtgtaaaag ctagcattga ttttcgagcg agctttgaag cgtagttttg acaaagcgta
    17281 tgccccgctg atcccaatca ctactgcagt tacggaggct atcaaggaga tcactaaact
    17341 atttttgaaa tacatgatga atggaaaaat ctcagcattg aaaatatcct gataatgttc
    17401 aaaagtccat tctttcggaa aaatagtttg tgtcgttgca attgcttctt gtgaatcctt
    17461 gaatgatgtc atcagcatga tcaaaaatgg aaacaaattg acagctaata gcaacaaaat
    17521 cgtcgcgtaa aataagattt tcctgctctt tttatttttc atttcaagtc caccttcttt
    17581 cgtgagagca agacgaccgc tgcaataatg atgaacaata gaatcgatat agctgctgct
    17641 tttccaagat cattgaacgc aaaggctgtc tgatacaaat aaacgcctaa gatattgact
    17701 ttattcgtca gtagatagac atctgtaaac atatagaaca tccagataga tcgtaaaaca
    17761 atcacagttg ctagtactgg tttgattgcc ggcagtgtca cgatcttgaa cttctgccaa
    17821 gcatttgcac cgtccatttc agctgcttca taaagcgttt tgtcgactgt ttgcaaaata
    17881 gccaaaaatg aaataaacgc atacggatag tatctccaga tagcaaacaa aacgaccata
    17941 aagaagctag caactggctg gtcgaaccac agcggtgctt cgttgaaaag ctgcaaccta
    18001 tctactaaca gatcattgac gattccataa ctattattga acatatattt ccaagcaaaa
    18061 atcaaagaaa tagatggcgt aacataactt aataaaatca gtgaacgcac cgttttccta
    18121 aatctgaact tgcggttaag caaaatagct acggctaatc ctaaagctgt actgcctgct
    18181 acaactaaga atgtgtaagc aactgttaaa cctaaagaac gataaaattc actatcactt
    18241 aaaatttcaa gataattgct taatccgata aaaacggcgt ctgagttagg attcagcggc
    18301 atatcgaaaa aactgatctg aatattggaa agcattggaa tcagcactaa aacagctaat
    18361 aaaagcatgc ttggtgctaa aagtcccatg gctaatttta agtcagaatg tttccttgga
    18421 cgattcattg aaacctcccc ctgataatct gccaatagct atctactttt ttcgaacacc
    18481 attggcagaa cttcttgtta tttcgtattt ttttgtgcgt cagctaaagc ttgatcgaca
    18541 tcttcgtttc cgacagttac ttggttcact gcttttccga tcacacctga gcttgtgata
    18601 tctcccattt tcgtgaaatt cttttccccg actagtccaa agacttgtac ttcatcaaaa
    18661 gcactagcaa tctcagatgg cagctcgcca aaagacttga tgacttcatt ttctttgtaa
    18721 ccatccagtt cgactacttg tttattcact ggttgagcac caccaggaga catcaagacc
    18781 cacttttcca tgtttttcgg ttgagataag aaagctgtga atgcttcagc agcctctttt
    18841 tgtttctcgt ctagaccaga agaaatcgtc aatccagata ctgtgccata gacagcttga
    18901 tctttttgtg ttgggatagc aaaaccgata ttttctgagt tccccgcttc ataaacagaa
    18961 ggcaaaatgt aagttgaata gattgccatt ggtgctgttc cattcatgaa agcatctttg
    19021 atttctgtca catcattaga gcctggcatc gtgtattggg ataagttttg ataaaacgac
    19081 aatgcttctc tcatcttttc cgtatcgatc gttacctcgc cttcatcatc caaaacattt
    19141 gctccatttg acaaagcaaa ttgagagaac gcttgctcac tcattgttcc ttcagcagta
    19201 ggcatagcaa ttccatattg tttattctct ttatctgtga aatgttgagc aatttttaaa
    19261 atatcatccc aattttctgg ttctgaaaaa ccagcttctg ataatttttc tttgtcatac
    19321 caaatacctt gaacccagcc gctaattggt gcagcaatat agctttttcc atcttctgaa
    19381 cgaacaaggt ttttggctcc gtcataataa ttatcttctc cagcttcctc gataacgttt
    19441 tggactgccg tctgatcaat cagttcatct ttatccatca ctttagcaaa atcctgactg
    19501 acttccatta cttctggtaa tttaccggaa cgagctaacg taacgatttt tgtattgaac
    19561 gctgattctt ctaccggcac ttgcttgatt ttgatgtctg gattttcttt ttcaaaatca
    19621 gcaaccagtt cattgatcac acttaatcgt tcctgttcga cagaagagtg catgaattga
    19681 atcgttgtct gttgtgattc gttagagctt tgactttcac cacaagcagt aagaagaaat
    19741 ccagtagcta gcagacaggt agtaacaata atttttttca tttttttcca cctcttattt
    19801 tattattttc agccacatat attcatacgg ctggaatatc aactttcgtt cttgactgca
    19861 atggataatt tgtccagaaa acaggtctag gtaatctcct tttggtaaaa gataacctgc
    19921 tgattgattg gaaaaattat ggataactaa gactttctct tccttcgctt cgcgaataaa
    19981 actgataaaa gaatctgctg agtccagtaa gtaaatcaaa ctattcggat ggaaggcttt
    20041 ttcttttttt cgaatagata gcatctgggt caattcacca tatactgctg ttcttaatgg
    20101 atctgtctgc aactctttct caatatctgc taacaaaaat ttttgccgat tgatttttcg
    20161 attctctcct gttttttcta cgcctttgta atcatttcga cttcccagca cactttgaat
    20221 ataaattgcc ggcacgcctg gtaacgttac caaaaatgaa tgagcagcta aaaagcgttt
    20281 gcttctccat tccgtcgtat ctgttggaga acttaacgca tctagatacg tagcgtttat
    20341 ctcatatgga ctggtcgttc catctgggtt tttcttgtag tttactcggg caccctcttt
    20401 cttcagctgt tccactaatt caaggatcgt tttttccggc agaatacctc gtaccggatt
    20461 caatccgata ccgtcatggg aggctaaaaa attaaaaaaa gttgtttttt tcgatgcgaa
    20521 atcgatggtt tttaaccatt ctttcaactt acttgtatta cccgaacgaa tcgcttgaag
    20581 ggtcaaaggt ggtagcgaaa actgatagac catctgtgct tcattctcgc cattcccaaa
    20641 ataactgata ttatctttgt gtggcacatt tgtctcagtg atcaataccg ttcctggagc
    20701 tgcttcatca gcgattgcac ggaataattg gatgatttta tgcgtttctg gcaggtgtat
    20761 acaagaagtt cctagttctt tccataaaaa tcccacagcg tccaagcgaa taaatgaagc
    20821 gcctttttct aagtaaaaca aaagaatatc gatcattttc aataaaactt ctggattttt
    20881 aaaattcaag tctacttgat cttcgctaaa agtcgtccat acgtgttttt ctttcccgtt
    20941 tttgtcaatg aacgttgtaa gtaacgggct ggtccttggt ctagtcacgc tgcttgtatc
    21001 tgtatccagt ggcaaatcaa taaaaaaatc tttgtacgta tcattcaagt ttaaatattc
    21061 ctgaaaccaa gtactttcgg cagacatatg attgcagaca aagtcgaaca ttaaccgtgt
    21121 ttcgcttctc atttcttcta tatcttccca tgtgcccaaa cggtcgttga ttgcctgata
    21181 atttacaacg gagaaaccat catcagatgt atatgggaaa aatggcaaga aatgtacaac
    21241 tctaaatgta tctgccaaat acttttgata aaatttctta aatgtacgca aagttggttg
    21301 ctgctcttca aaaaattgat ccccataagt aatcaagaaa atatcttctt cgtcccaatg
    21361 cagtttgcct gcctggcctt tcttctgctg ataaacactg actttttgtt caatcataga
    21421 ggcggttttc tcagacaaat ctgagtcgcg ataaatcgct tgtaaaagtt ttgtatacat
    21481 cggattcccc tttctttttt gggagcgttc ccaacgattt gatcatatta tggaaaacgc
    21541 ttactgtcaa tagcattttt ttaaataatt ttgatataca caaaaataca aataagaaag
    21601 cttatgtatc aataattaat agcttcttaa aaaaatatgg tctctcatga tcatgcaagc
    21661 tattcctaaa ttttcaaaat catagacaaa aaagaatgtc tattgtacta tggaattatt
    21721 ctaaaacgct cccaaaaagg aggtgcatat aaaattgaaa cctactattt atgacattgc
    21781 gaaaaaagct aatgtctcaa aatcaactgt ctccagagta ttaaataacc agccaaatat
    21841 ctctgataaa gcaaaagcaa gtgtacttca agcaatcaaa gaattgaatt atgaaccgag
    21901 caaaattgca agaggactgt caggaaatgg ttttgacgcg attctagcat tatctcatcg
    21961 tacttctcat acgacctatg gcaatccttt tttttcagat gttattcaat ccatttctgc
    22021 cgtttctgaa caaaataact tcgacttgat tctacaaacg gcaaaaacac cagaagaaga
    22081 gctagaaaaa tgcgcgaaaa agatccaaga aaaaatgatc cgaggaatta ttattttaag
    22141 ctctacgatg gatgaaacac ttctttttga attggatcaa tacaatattc ctattgtggt
    22201 aatcggaaaa gtcggaaacg actacacgaa catctactca gtggacacgg ataatttcca
    22261 ggatagttac gatttggtgg aaacactgat caagaatgga caccgtcata tcggctgtct
    22321 ccatgctccg acagaagttt atgtatccaa tgatcgtctc aatggttacc gtcaatgctt
    22381 atttgatcac cacctagata tccgtaatga atggattata gacggcggct ttcgcttaga
    22441 agatagcatg gcagcaatga atgatttaat gaagctacga gaaagaccaa ctgcgatttt
    22501 ttctaccgat gcattaaaat cattgagtat ttacaaagtg gcagaacaat ctggaaagca
    22561 gatacctgat gatctttcga tcgcaggatt cagtgatccc ttattgtcct ccctattttc
    22621 gccacagttg actcggatag acattccgac aaatcagtta ggaatcaaag ccacagaact
    22681 tttattccaa ctaatccacc accaataccc tgaagaaaaa agaatcattc tttcaaccga
    22741 gattactctc acagattcga tccaacaaca agaaatgtag aacagaatta taaaagaaca
    22801 gaaacactga ggataagtca gttactctgt tcttttttca atcactcatc cctcgttacc
    22861 ccctcccggt attttagcga atttttctta ttttccatct gttttagata gctgtggata
    22921 actataatgt ttgtgttaaa ctattttttc cacagggaag aaaaaactat ttttaaccac
    22981 gttgtgtata agtttttata gttcgttttt ttaaacacat ttacccttat ttcaatccat
    23041 gttatttggg gatacttatc tctaaaacat aaatagaata acagacatgt ctactggttt
    23101 tgttttattc gaggaattat tcaataatag ctagcaaaat attaaaaata accgctctat
    23161 cttcccatca atttattttg cattcaaaaa atgatctatc tttacttttg ttgattgact
    23221 tctcttggaa gatgatcgtt ccgatacctt aattggatac gaaccgatgt tgttaagatc
    23281 ctttcccact tttcttctag ttagacaaat tattttagac aaaccaaaaa agaggttgtg
    23341 acaataattt gtcacaacct cttttttaaa atatctattt tgtttgattg ttcattgcgt
    23401 cttctgcagc catttgcgct tcaatatctg aaatactgta gacattttcg cttgctacac
    23461 cgacttcttt tggttccatg tcacgataac gttgcatacc agtaccagct gggatgatct
    23521 tacctataat aacattttct ttcaaaccaa gaagaggatc tttcttaccg cggatcgcag
    23581 catccgtcaa gacacgagtt gtttcttgga atgatgcagc agataagaaa ctatttgttt
    23641 ctaatgatgc ttttgtgata cctagtaaga caggtcgaga agttgcagga acgcctcctg
    23701 agactagcgt attgtagttt tgatctttaa agtctgcgat gtctaataat gtacctggta
    23761 agatatctgt atcacctgga tccattacac gaactttacg cagcatttga cgaaccatta
    23821 cttcgatatg cttgtcgccg atttctaccc cttgcatacg gtaaacacgt tgtacttcac
    23881 gaagcaagta gttttcaaca gataatacat cgcgaacttg caacaattgt tttggatcga
    23941 ttgatccttc tgtcaatggt gcaccacggt ggatcatatc gccttctgct actttcatac
    24001 gtgctgtata aggaacagta tacgtacgtg tatctgtttt tcctttgatt gttacttctt
    24061 tggtacgtgt tgcaggatct tctgagatat cgatgacatc ccctgttact tctgtgatca
    24121 ctgcttgccc tttcggatta cgtgcttcaa agatttcttg gacacgagga agaccttgag
    24181 tgatatcgtc tccggcaacc ccacctgtat ggaatgtacg catcgtcaac tgagtaccag
    24241 gttcaccgat tgattgagcg gcgatcgtac caactgcttc tccgacttca acgtcagagc
    24301 cagttgcaag gttgcgtccg taacaatgtt tgcagacacc atgttttgta ttacatgtga
    24361 agacagaacg gattgtcact gtttcgacac ctgcatcaac gatttgtttt gcgatgtctt
    24421 cggaaatcaa ttggtttcct gcaatgatca attcatttgt ttcaggatga cgtacttctt
    24481 tacgggtgta acgtcccact aaacgatctt ctaatgattc gatgatttca tttccctcgc
    24541 ggatggcttg gatttcaaga cctcggtcag taccgcaatc ttcttcacgg atgattacat
    24601 cttgggccac gtcaaccaga cgacgagtca agtaacctga atcggcagtc ttcaaggctg
    24661 tatcggtcat ccctttacga gccccgtgag ttgagataaa catttccaag acggatagtc
    24721 cttcacggaa gttcgaaatg attggcaatt ccatgattcg accattagga gcggccatca
    24781 aaccacgcat tccggcaagc tgtgtaaagt tggaaatgtt accacgagct ccagaatcag
    24841 acatcatgaa gattggattt ttcgcttcca aaccttccat cagtttttgt tggatgctgt
    24901 cttttgcagc attccaaaca gcaatgacac gttcataacg ttcgtcatct gtgatcagac
    24961 cacgacggaa ttgttttgta atagtttcaa cttgtttatg ggcattttca atgatttctt
    25021 gtttttcatt cagtacactg atgtcagcga tccctactgt gataccggca tgtgtcgaat
    25081 gtttgtaccc taagtctttc atacggtcaa gcatcttaga agtttctgtt actttgaatc
    25141 gtttgaagac ttctgcgatg atgtttccta agtttttctt cttgaacggt aatactaatt
    25201 cttgctcttt gatatgtgca ggaatatcag taccagcttc aacaaagtat ttgtccggtg
    25261 tttggacggt caagttgaag tctgttggtt cattcagata agggaattca ggaggcatga
    25321 tctcgttgaa gatgatctta ccaactgttg tgatcatcgt acgttctttt tgccattcag
    25381 taaatggttt ttcacctaat gagttagggt tcacaccgat acgtgaatgt aaatgaacat
    25441 atccgttgcg ccatgcgata acggcttcgt tttgatctct gaagaccatt ccttcgcctt
    25501 cgcgtccttc ttcttccatt gtcaagtagt agttacctag aaccatatct tgagaaggag
    25561 taacaactgg tttaccatct ttagggttca agatgttttg agcagctaac atcaacatac
    25621 gtgcttctgc ttgtgcttct tcattcaatg gaacgtggac agccatttgg tctccatcga
    25681 aatcggcatt ataagcttca catacaagtg gatgcaaacg gattgcgcgt ccttgtacta
    25741 aaactggttc gaatgcttgg ataccaagtc ggtgaagcgt aggtgcccgg ttcagcaata
    25801 ctgggtgttc cttgattacg tcttctaaaa catcccatac ttcgtcttct tgacgttcga
    25861 ttttacgttt cgcatttttg atgtttgaag ctagttcgcg ttgaacaagc tcacgcatca
    25921 cgaatggttt gaataattca atggccattt cttttggaag tccacattgg tacatcttca
    25981 agaaaggtcc aacaacgata acggaacgac ctgagtagtc gacacgttta cctagcaagt
    26041 tttgacggaa acgaccttgt ttccctttca acatatgaga aagagatttc aatgggcggt
    26101 tacctggtcc agtaactgga cgtccgcgac gaccgttatc gatcaatgca tctactgctt
    26161 cttgcagcat acgtttttca ttttgaacga tgattccagg tgcattcaag tctaacaaac
    26221 gtttcaaacg gttattacgg ttgattacac gacggtaaag atcattcaag tcacttgttg
    26281 caaaacgtcc accttctaat tgaaccattg ggcgtaaatc tggtggaata acagggataa
    26341 cgtccataac catccagctt ggttgatttc ctgaagcgcg gaatgcttct aaaatatcca
    26401 aacggcggat tgcacgtgta cgtttttgtc cttgtgcaga tttcaattct tctttcaatt
    26461 cggctgcttc tgcatctaga tcaacattat caagtaattg tttaatcgct tctgcaccca
    26521 tagcagcttg gaattcttgt ccatattgtt cacgtttttc gcgatattcg cgttctgtca
    26581 acaattgttt tttctctaaa ttcgtatttc ctggttcgat gactacatat gaagcaaaat
    26641 aaatgatttc ttccaaagcg cgtgggctca tgtctaatac aagacccata cgtgaaggaa
    26701 tccctttaaa gtaccagata tgtgaaacag gagctgctaa ttcaatatgt cccatgcgtt
    26761 cacggcgaac tttcgaacga gtaacttcca ctccgcatcg atcacaaacg attcctttat
    26821 aacggatacg tttgtatttt ccgcaggcac attcccagtc ttttgtagga ccaaaaatcc
    26881 gctcacagaa caacccttcg cgttcaggtt ttaaggtacg atagttaatc gtctcaggtt
    26941 tttttacttc accgtaagac cagcttctga ttttttcggg agaagccaaa cctatttgca
    27001 tactttcgaa tttatttaca tcgatcaaaa ggggttccct ccatttcatt taatggcaaa
    27061 ccgcaagacg gctgatcttc gaccgccttg cagctcatag accggattag tctaaattat
    27121 cttctgcggt ttcaaagttt tggatgacat cgtctttttc atcttccact tgttcagcag
    27181 ctttcttttc taattctttg gcagtttgct gttcggcaaa ttttgtcaaa gcatcaacag
    27241 tgatcaaatc gtcgtcttca tcgtccatgt cgcgaagttc gatctctgtt tcttcgatat
    27301 ccaagacacg catgtccaga cctagtgatt gcaattcttt caccaatacg cggaatgatt
    27361 ccggtacacc tggttttgga attggttcac ccttgacgat tgcttcgtat gttttcacac
    27421 gtccgactac gtcatctgat ttgtatgtca agatttcttg taaagtataa gccgcaccgt
    27481 aagcttccag tgcccatact tccatttcac caaaacgttg tccaccaaat tgagctttac
    27541 ctcccaatgg ttgttgtgta accaatgagt aaggtccgat tgaacgtgca tgcaatttgt
    27601 catcgaccat gtgagcaagt ttgatcatgt acatcacacc gacagacaca cgtccatcaa
    27661 atggttcacc tgtacgtccg tcgtacagaa cagttttcgc atcgctggcc ataccggctt
    27721 cacgaactgt ttcccatacg tcttcatcac ttgcaccatc aaatactggc gttgctacgt
    27781 ggatacctaa ctgacgtgca gccataccca agtgtaattc caatacttgc ccgatgttca
    27841 tacgtgaagg cacccctaaa ggattcagca tgatatcgat tggtgttcca tcaggtagga
    27901 atggcatatc ttcttccggc ataatacggg atacaacccc tttatttccg tgacgaccgg
    27961 ccattttatc cccttcatgg attttacgtt tttgaacgat ataaacacga actaacatgt
    28021 ttacgcctgg tgacaattca tctccagctt cacgagtaaa gattttcaca tcgtgaacga
    28081 taccgccgcc gccatgtggt acacgtaatg atgtatcgcg gacttcacga gctttttcgc
    28141 caaagatcgc atgcaataga cgttcttctg cagataattc tgttacccct tttggcgtga
    28201 ctttccctac aagcaaatcg ccatcttgga cttctgcacc aatacggatg atacccattt
    28261 cgtctaaatc ttttaatgcg tcttccccga cattagggat ttcgcgtgtg atttcttcag
    28321 gtcctaattt tgtatcgcgg gcttctgatt catattcttc aatatggata gaagtgtata
    28381 cgtcatcttt taccaaacga cggctcatga tgatcgcatc ttcatagttg tacccttccc
    28441 aagtcatgaa accaactaaa acgttttggc ctagagccat ttcgccttgt tccatagatg
    28501 gtccgtctgc cagagtatcc cctttttcga ctttctcacc caaatgaacg attggacgtt
    28561 ggttgtagct tgttccggag tttgaacgac ggaattttgt tacatcgtat ttatctaatg
    28621 caccgttgtc gcggcgtacg cggatttcag aagcatcaac gtattcaacg acaccgtcat
    28681 gtttacataa caaagcagct cctgagtcat gcgctgattt gtattccata ccagtaccaa
    28741 cccatggtgc ttgaggattg atcaacggca ctgcttgacg ctgcatgttg gcacccatca
    28801 aggcacggtt ggagtcatcg ttttccaaga aaggaataca tgctgtcgca acggcaacta
    28861 cttgtttagg tgatacgtcc atgtagtcta ctttatcaat agatacttct aagttttcac
    28921 tttgagcacg agccataaca acgtcttgag caaatgttcc atcttcattc aatgggctgt
    28981 ttgcttgggc tacgatgtaa tggtcttcga tatcagctgt caagtagtcg atttggtcag
    29041 taacacgacc tgtttcacgg tctacacgac gataaggtgt ttcgataaat ccgaatttat
    29101 ttactttcgc ataactggac aaactattga tcaacccgat gtttgggcct tcaggagttt
    29161 cgatcggaca catacggcca tagtgggagt agtgaacgtc acgaacttca taaccggcgc
    29221 ggtcacgagt caatccgcca ggtcctaagg ctgaaagacg acgtttgtgt gtcaattcgc
    29281 caagagggtt tgtttgatcc atgaactgtg acaactgaga agaaccaaag aattctttga
    29341 tacttgccac aactggacgg atattgatca attgttgagg tgtcaatgtt tcagtatctt
    29401 ggatcgacat tctttcacga accacacgtt ccatacgtgc tagcccgata cggaattggt
    29461 tttgtaaaag ttcaccgact gaacggatac gacgatttcc taagtggtcg atatcatcga
    29521 cattgccgat accttccatc aagttcaaga aatagctcat tgaagcaatg atgtcggctg
    29581 gacgaaccgt ttttacagct gcttctggat agccgtttcc gatcacgttt acttcacgtt
    29641 cagggtctct tggtgaaaat actttgatga cttggacagt cattggatct gtgaccacac
    29701 cgtcttctga tggatagtaa gtcactgaat ttaggccatt atcaatgaaa ggagctagtg
    29761 tttccatgac ttggtgagtc agaactgttc ctttttcaac gatgatctcg cctgtttctg
    29821 gatcaactaa tgtttcagct aatgtcagat tcaatagacg tgttttcaaa tctaattttt
    29881 tgtttacttt gtaacgacca acgtttgcta agtcgtaacg ttttggatcg aagaaacgag
    29941 cattcagcaa gttacgtgag ctgtctgctg tttttggttc gcctggacgc aaacgttcgt
    30001 aaacgtcttt caaaccttct tctgttcttg agtcgcttgc gtttttatgc aagtcttttt
    30061 cgactgtatt gcgtaatgtt tcgctgtcgc cgaagatttc aaagatcgta tcatcggaac
    30121 caaatcctaa cgcacgaact aatacggtta aaggaatctt acgtgtacgg tcgatacgta
    30181 cataagagat gtcttttgca tcagtttcca tttctagcca agctccacgg ttagggataa
    30241 cagtagaacc gaatccttct tttccgtttt tgtcgacttt tccatggaaa tagacacctg
    30301 gtgaacggac taactgagaa acgattactc gctctgcccc gttgatgatg aatgttcctt
    30361 gttctgtcat taatgggaaa tcaccgaaga atacttcttg tgctttgatt tcgccagtct
    30421 cgcggtttgt caaacgcaat gttacatgta atggtgcaga gtagtttgca tcatgcgcac
    30481 gagcttctgc tactgtatac tttggttctt tcaattcata atctacaaat tccaaagata
    30541 gattgccatt gaagtcatcg attggcaaaa tgtcttcaaa catttctcta agtccctcat
    30601 ctaagaacca ttggtaagag tctgtttgaa tttcgatcaa atttggtaat tccaacactt
    30661 cactgattct tgcgaaactt ctacgttcgc gatgttttcc gtattttact acgtgtccag
    30721 ccaagctctt cacccctcta aaatgtcatt caaaaagact acccgatcaa ttgttacaat
    30781 aatatttcaa atattatttt taggtaacaa cagacatttt ttgcggcttt taggcaaaaa
    30841 aaaaccaaaa atagaacatt ctcttgaaac cttacttcgt gagcttctac ttttgtttcc
    30901 ttatattaaa gccgaaacgg acaatatttc gtttctgcct tttcttcatc agatagtttt
    30961 tgcaacttca tatactacac ttattttcta taaaagtcaa cacttcctgg cgtttcttct
    31021 gctttttcgc aacattttct taatgttttc aattttatgt tttcaattat ctgacaattc
    31081 ttaattgaca gaccttttta tctcggttat actttagaaa attcattttg tggaggaact
    31141 tctatgcgaa ccaacatcaa ccaaatcgca ccaacacaac tgaataactt ttactttagc
    31201 tactttagat aggtttgtat tcatcatgat ggatacaaac gttaaacgac agtttgtatc
    31261 tatgatggct aaagaacttt gtattgcagc aaaaagccac gtagacaaaa tgctaagtgg
    31321 ctgagcagtg aaaatacaaa gtaaaagtat caagcattct actttttcaa tttattttcc
    31381 gacttttcaa tccatttgga cattttgtct gaatggattt tttgtttccc aaaattaagg
    31441 aggaaaaaga aatgaaaaaa aatataatca tcggattagg cgctgccctg ctactagctt
    31501 taggaggttg cgcacaaaaa gacaattccg cagaggcatc agatggacaa aaaacaattg
    31561 gcgtattgca acttgtacaa cataactcgt tagatgctgc ctataaagga ttcaaagaag
    31621 gattagcaga agaagggtat aaagaaggcg ataatctaac aatcgattat caaaatgccc
    31681 aaaacaatca agacaacctg aaaagtatga gtgagaaact cgtcaaagaa gatcctgatt
    31741 tgctacttgg aatcgccact ccagcagcac aatctttatt aaatgaaaca accactatcc
    31801 cgatgactgt cacagcggtc acagacttgg aacaagccca tttagtcaca tccaatgaaa
    31861 aacctggcgg caatgtgact ggcacgacag atatggtgcc aatcgaaaaa caaatcggct
    31921 tactgttaaa tatcgtgcca gacgccaaaa caatcggcat catgtataac aacggtgaag
    31981 caaactctaa gatccaagct gatttggcag aaaaagaatt gaaaaaagcg ggcgtcaaag
    32041 tcaaagtact gacagcaaat tccacaaatg atgtccaaca agtgacgaaa agtctagcta
    32101 aagatgtaga tggaatctat attcctactg ataacacgtt cgcaagtgcg gcaagtgttg
    32161 tcggtgaagt cgcaaaagaa acaaaaacac ctgtcgttgc tggcgcagtt gatcaagtca
    32221 aagacggcgg tctagcaaca gtaggaatcg actacgaaag cttgggcaaa caaacaggga
    32281 tcatggcagc aaaaatctta gacggcaaga atccaagtga acttcctgtc gaaacagcga
    32341 atgatttgga actgtatgtc aataaagaca tggccaaagc attaggcatc aatccagaca
    32401 gcatcaaaga agctgaataa aacacaggag gattctcatg acaaatttgc tctctctttt
    32461 cttatcatct gcatcgcaag gggtcttatg ggccctactt gcgattggtg tatacgtcac
    32521 tttccgtatc ttggacattg ccgacttgac tgctgaagga agctttcctt taggtgcagg
    32581 gatcgcggca gtaagtatta caaatggcta tcatccattg acagcttgtc ttctaggctt
    32641 tttagggggt gcggcagcag ggcttgtttc cggactgctt cacacaaaat tgaaaattcc
    32701 cgcccttctt gcagggatca tcaccatgac tggtttatat tctgtcacat cccgtgtgat
    32761 gggtgcgccg aacatctctt tattgggtca gccaacgatc tttcattggg tcgagtcatt
    32821 cggcataaca aaagacaacg ctgtactgat cgtcggtctg ctttttgctt tgatcgttgt
    32881 atgtttactt gttttgttct tacgtacaga aacaggactt gctattcgtg caactggcga
    32941 taatctggca atgagcgaag caaacgggat caacacagat aatatgaaga tcatcggtta
    33001 tatgatttct aatggatgca ttgctctttc tggctccttg cttgcgcaaa acaacggctt
    33061 tgctgattta aattctggta ttggaacgat cgtaattgga cttgcttcta ttatcatcgc
    33121 agaagttttg ttacgtaacc agccactatt tctccgtctc ttgactgtcg ttttaggtgc
    33181 ggtcatttat cgttttattt tagcgttagt cttcgaactg aatgttgaac caagtgattc
    33241 gaaacttgct tctgctttcg tgctagtgat ctgtctttct ttcccgcaga tccagcaaaa
    33301 attccattta cctcaaaaaa agaaaggcgg tagtgtcaaa tgaatgacgt cttaacattg
    33361 gaaaatctcc atcatacctt tgaaaaagga acgatcaacg aaaatcatgt cctgaaaggc
    33421 attaatcttt ccttagatcc cggcgacttc gtgacgatca tcggtggaaa tggggctggt
    33481 aaatctactt tgctgaacag catcgccggt acattttctg tcgatcaggg tcaaatttta
    33541 ttgaatggaa aaaatatcac gaaaaaatct gttgtcgaac gttcgaaatc catcagtcgc
    33601 gtattccaag atcctaaact agggacagcc gttcgtttaa cggtagaaga aaatcttgct
    33661 cttgctatga aacgaggaaa aaaacgcggc ttcttccgtg gtgtcaaacc acaagaccga
    33721 acatttttca aagaccagct ggttcggcta aatttggggt tggaaaatcg tttatcctca
    33781 gaaatcggtt tgctgtctgg aggacaacgt caagctatta cgttattgat ggccacatta
    33841 cagcgtccag aactgatctt attagatgaa cacactgcag cgcttgaccc tgctacttcc
    33901 aaaacggtga tggaattgac agaaaagctg atcactgagc agaatctcac tgcttttatg
    33961 gtcactcatg acatggaaga cgcgatccgc tatgggagtc gactgatcat gctgcatcaa
    34021 ggaaggatcg tcgtagatat ccagcaagag gaaaaacagc agctcacagt gccccaatta
    34081 ttagcgttgt tcaaacaaaa cagcggcacg gcattaaaag acgatcaagt acttttatct
    34141 tcataatcag aaagaggatg ggacaaaaaa tgaacgccca ccctcttctt ttttctcttt
    34201 ccttctttcg tttttgcttt atgtatcaac cgttgtttct tcgaaaacta ttttgttctc
    34261 tccttcttcc tgatagaatg gatgggaaag gagcttgatg ccatgacaac gaaagacaag
    34321 gtacttcaat tattgaaaaa gacaccagat tttctttccg gagaaaagtt ggcacagcaa
    34381 ctggaagttt cccggacaag tatttggaaa gccatcaaag aattggaaaa ggcaggctat
    34441 cgttttgagc acgggcctac agggtatcgt tacctgccct ctgacgtact agatgcaaaa
    34501 gaaatccagc aagagctacg gtcgtttatt cctgatttga aagtacaaac gatgttgact
    34561 tctgaatcta cgatgaaaga tgccaaactg gcagcagctc aaggagattc tgtttcgaag
    34621 ttatttgttg ctgatacaca agaacaagct cgtggacgtt ttggacggcc ttttttcgct
    34681 gaacctggac gaggaatcta catgagtctc ttgctccatc cagacaaacg gtttgatgaa
    34741 ttgcctcaat atacgatcat cgcagccagt gcctgtgtga aagccattga atctgtaacc
    34801 ggtcgttctg cttcgatcaa atgggtcaat gatatctaca tggatggaaa aaaagtctgt
    34861 gggattttat ctgaagcagt cagtgatatg gaaagcggca ggatcagtca tatcatcatt
    34921 gggatcgggc ttaatttttc tgttcctcaa aatcattttc cagctgatct ccaagaaaaa
    34981 gccatctctg tctttcccga cggacaagct agtgtcacaa ggaatcagct aatcagcaaa
    35041 atctggcaag aattgttcac ttatctttct cacccagaaa aagcgttgga cttctataaa
    35101 gaaaaatcat tcgtattggg aaaaaccgtg tcgttcaatc aaaaaggaac gacttatgtc
    35161 ggtcttgcaa aagaaatcgg tgatcatggc gaactgatcg tccaaacaaa tgaacagaag
    35221 atgagtttgt cttctggcga aatcagtctc acatctatcc gtacgcaata attatcagat
    35281 ttcacaaaag gacacctgat ctcatacagt gtcctttttt ctgaattctc ttcaaaagac
    35341 tctccttatt ctatcagctg acagtttctc tatcctctag aaaaaggtat tctaaaaata
    35401 tctataaagc atggtacact ttctttaaat gaatcaatgc aaaaaacaag agagtcatca
    35461 gtttacacga tcaaaagaga tagcaggccg cttccttttt caatcttata gatagaccat
    35521 gggtaacgat aaaaacaaaa aagaacttgc gacttatttt gccgtaagtt cttttttagc
    35581 tagtgcctta atctcttaat cccaacaaac gtctcgcata gatcacctga ccagagtgca
    35641 tagcagcatc atcaataata gaaacgagac gcattcctcg tgtgactggt ggtgtccagt
    35701 tctcgtcaat tacttcatct agctcggaag gttccagcct tcttaagtaa gaaatagcaa
    35761 aatcagcagc tcctcgcaag tatcctagca gatcttctat ttgattagct tggatctgct
    35821 ttgcttgttc cagactatgc ttccaatctt gttcgttatc tgctatcgtc atcggaaacc
    35881 ggctttccca gtcttgacta tgccaaattg gagaagttcc tgctaaatca gcaatctgga
    35941 aatctaattc tcgagcggta tgccaagcca gccaagtcat tgatttgatc tgcggggctc
    36001 tatcttccac tggaaaacga ttggcctcct caatagagac gccttcaaac aaacgttcaa
    36061 accgatcgta tgcccgatcc acactctcgc atagcatatc taaataatcc atttattttc
    36121 ctccttttta tttatcctac ttactttttc ctaattctag gatacctact tatatgttac
    36181 tacacaccct tattttttac actttaacag tcaccatcat caatagtttg gagattgaaa
    36241 gaaaaagata gtgtcgaact atcaattgaa caagaagcaa caatatgaac gtttcattaa
    36301 tattctttaa ataaacaatg atttttcaaa cgaacagttt aagactagga gaaaaagagt
    36361 ataataggaa aatgtttgaa aggaaaggaa ggggaaaaat gaaaaaaagg gcaagtttac
    36421 tagtcctagt tgctttgttg ttcaatatct tttcgtatgg tgtggccgtt tcagcggaaa
    36481 catacattcc taaagaccct gggaatattt ataatgatct aactggtaac tatggcttgt
    36541 taggaattgc tagtcaattc cacgtatttg ctaaaaataa gacaactgtg aatgcacata
    36601 cagacggaaa tatagcaaca catgaacttg atgcaaaaaa taattttggt acagagcttc
    36661 atgaaggttt attgaaccaa gaaataaatt atatacaaaa aactgattct ttcgctaatt
    36721 cttcaggcat acctacaggt agcacaagaa tgaataaatt tgtcgttggc agtaacgtgg
    36781 atgtagcact tcaagatggt catcctacag tcaatggatc caggcttgat cacttaactc
    36841 cagatgattt ttataaagat cgtgctgggc atgaatatat tgatttcgag caggaatttg
    36901 ctaaattgaa tgtagcagca aatgatcttg caacgatcac tccagctaaa acatatactg
    36961 ctgcagattt tccagacatg aacaatcgga caatcgattt aactgattta tcagataccg
    37021 ggtttttatt ggtaaatatc gatgcagaag tgttaacgat gaatacgcca cttcaaatca
    37081 tgaaacagaa gatttttcgg atgcgaatat ctcttggaat tttggaaatg agatgactga
    37141 tttgacaatc agtgctcctt ttcaaggaac aatccttgcg cctaatgcta ctatccgcat
    37201 tactcaaaac caagacggaa cgattatagg aacaaatgtg attttagatg cagctaccaa
    37261 tcgttgggat cctaacgaaa tatttatcag cgacaatggt acagatacga cagacacaag
    37321 cgacagtaca gatacatcta ccacaagtga tagctcagat tcatcaactt caagcgacag
    37381 tacagatact acctctacca caagtgatag cacggataca tcagcttcaa gcgacagtac
    37441 agatactacc tctaccacaa gtgatagcac ggacacatca gcttcaagtg acagtacaga
    37501 tactacctct accacaagtg acagcacgga cacgtcagct tcaagcgaca gtacagatac
    37561 tacctctacc acaagtgata gcacggacac atcagcttca agtgacagta cagatactac
    37621 ctctaccaca agtgatagca cggacacgtc agcttcaagc gacagtacgg atacatctac
    37681 cacaacaagt gatagcacgg acacgtcagc ttcaagtgac agtacagata ctacctctac
    37741 cacaagtgat agctcagatt catctaccac tacaagtgat agcacggaca cgtcagcttc
    37801 aagtgacagt acagatacat cctctaccac aagtgatagc tcagattcat caacttcaag
    37861 cgatagtaca gacacgtcgt ctaccacaag cgatacacgt catgcaagtg tctccttctc
    37921 aaaaacaagt tcggataaca ggacgaactt taacttaact aataaaacaa gcaatgacaa
    37981 tggaaacaaa tcaaaaagag cacttcctaa aactggaagt caatcgaata attggataac
    38041 actcgcaggc gttatattac ttgtaatcgg tttaagaatc acttttatga gttataagaa
    38101 atcgaaaagg taaaaaaata ttcaagataa aaaagagcaa cgtcgctaac ggacgttgct
    38161 ctttattttt gctttttatc ataaatacaa gaactacaat tcaatttgtc aaaaaacacg
    38221 tagatttcct tctgagaaat ttatttacgt atcgaacctt tccctattca gtctttgcga
    38281 tccaaaatca gttgggtaag ggaaaagaga ttctcttttg ttcgaagaga ggtggcaggt
    38341 aatttcttta tttccttcaa agccttttct gtgtaagagg ccgctaactt cctagcttct
    38401 tccacgcctc ctgtatgttc aacgattttg cgtacggcgt cgacatcttc ttctgtcatt
    38461 gccgctttct tctctaaata aggtagtagc tttggtttgt ttttttgcaa agagtagatc
    38521 aacggtaaag aatagacccc ttgacgcatg tcttctagta caggtttgcc gatttcctgg
    38581 ctctcttttg tataatctaa aatatcatcg ataatctgaa aggccatccc gatagaaagt
    38641 ccgatatctc ctgctttttt tgcgaagcgc tcgcttgttc cactttcaaa agcgccaaca
    38701 gaacaactta attggaacag ttcagctgtc ttacctgaaa tattttccaa atattctttt
    38761 acagaaagtt cataattata gcgatcatcc atctgcccta attcgccatt caatactttt
    38821 tccatactgc gagaattcaa ttggagactt ttcaaggaag tggagtaatc agacaatagt
    38881 ttaaaacagc agacaaataa atagtctcct gcatagacag aaacgacatt accaaactga
    38941 ctacggatat taggctgacc acgtcgcaga tccgcttcat caacgatatc atcatggatc
    39001 agtgtggccg tatggagcat ttcgatagaa gctgccaaag cgatggcttt gtctcgatct
    39061 tgctctggtc caaattgtga gaacaatagc tgataggcag gacgcaacag ctttccgccg
    39121 gcatggatcg tttctttgat tgcttgttcg acaggtttgt ttttcaggcg gacagtttct
    39181 tccatccgtt ttagtgtttc tgtcaattct ttacttaaag caggatagtt ttcccacatt
    39241 ggatgtagct tcataaaaaa actccttgtg tgacgattat tttttggtaa acattgggct
    39301 ccatttttgt ttggttccct gctcataatc tttttggaat cgttgaagtg tctgcacatg
    39361 tcgtaaaaga taattcgctg cgatcccgat tgcaatccct gataaaatcc ctaaaactga
    39421 aaggactggc aagtacaaca tgactgtcca tgattgtgca atgaaagaag ccatcaaaag
    39481 ctgtccgaca ttatgcatga aaccgccggc cgcactaatt ccgatgatac tgacacgttt
    39541 tggtcctaat tgcttgacca ataacattcc aaaataacta agcaaagcac cactcatact
    39601 ataaaggaat gtggaaatcg ttccgcctag caaagtagtc aggaatagtc gtagccaaag
    39661 tagtaagaag ctgtctcgtt ttttcatggt aaaaatggca acgatcgtga ttagattggc
    39721 taagcctagt tttgcaccag gcgcaaaaga aaaaggataa gggatcatat tttccaataa
    39781 accaatgatc actccttgtg caacaagtag cgaaatataa atcatttttt gtaatttact
    39841 catggtgtgt gaaggtctaa taaatcagac tgtcttcgtc acctccatct gttgaacgga
    39901 cttcgatcac taatttatgt ggcaaacaga cgatggtttc gccatttttc gttgcccagc
    39961 ctcgacgtac gcagatctgg tcatcgcaat ctgcttcttt tatccgtact cgatcgccat
    40021 ctatctcgat caagttataa tctccgtgtt catctttgta agtatatgtg ttgctctctt
    40081 ctccagccat caaagggaat gttttgattt ctgtgccatc cacacgcagt accgcttctt
    40141 tttctgctgt tgcggtgttt tgctgaagac caaaaatcat gactggtaaa aaagaaagaa
    40201 cagtcaaaag caaaataata aaaacatccc aaggtttgag cagacttttt tcagcaaatt
    40261 ctttaaggct catttttgtt ctcctttttc cagattaact cacccaattt tagcataaaa
    40321 acaagaggtt gtgacacctg tgtcttgctt tgagtaataa gaacgaagaa tagaaaacag
    40381 ctttccatgt tttctaattc tttgggctta ttaccgagaa gcagcaggtg gaacaccgtt
    40441 tataacaaga ggttgtgaca gaagtgggca acttcaagaa ataagaagga atttacgaaa
    40501 attacttttc aagtttttgt gaaatttcgg cttatttttc gaagaagcta cttctggaac
    40561 accgtttata acaagaggtt gtaacagagg ttgtgacacc tgtgtcttgc tcatggaggc
    40621 ttatctcggg catcttattc ctttaatttt acataaaaag gaggctgtga cggtttgtct
    40681 cagccccttt ctattgaaat cttgcattca aataacgttc tttatctatt tatttcttgc
    40741 cgtactggga acttggtcta ccgtaccacc aattacctag tgtacgttgt agccatggaa
    40801 ttacccagcg atccaaccca atcgcacgtc cggaaccgtt catcaatgcg aaagcaacaa
    40861 aaatgaacca aatgtttacc cagtaaaaca tgcctgataa gcagaacgtg atcaccaata
    40921 caatcgttgt cgcactgcat agccatgtga ataatccagc aatcaatgct aatgccaagg
    40981 caatttcaaa gaataccatg aatttttgga agaacattgc tacatcattg tttggcatga
    41041 tcacttgcat gatctttgtg aaccagtctg gtgacttctc aatcacagcc attggttctt
    41101 caccgtatgc ataactcaaa ccaaaatgtg cagcttgagt cgctgcttct ccggcacttg
    41161 cccctgctcc gctggcagct gtggttgcat caccagcagc agaagcacca ctggcaacat
    41221 ctgttacatt gtattcccat gtccaaggga acacactgtt tccagtgaac catgatcctt
    41281 caccaaacca agtacttggt ttgaacactt caccattccc aatgattttt ttggaagctt
    41341 caacgaacca aactaaacca tagaaaatac gaagtggcac actccataag acattgccgt
    41401 aacgagaaga atgtcctcga gtcacgttgc gatcatcttt gatatggaaa aactcatgca
    41461 ttaaatattg gaacatataa taaccagaac gaatatcaaa gaagtatttc aaattcacga
    41521 tatgtttcat tacgatagct aaaaatccgc ttaaatggat cttatcaaat aggttagcta
    41581 ccccccattt ggcaccaata gaaaccataa atccttgata gtttccttta aatttatgct
    41641 tttctccgcc tttgatcgaa gccacgatat tcgctgcggc tgtgtgtcct gtttgttcag
    41701 cagcttgaac gatctgcggt gtcggtgttc ctgggaattc ttcataaaag actaaatcac
    41761 caatgatata gatgttttta tcttcgtagc cttttgcctg catatattca ttggcgatca
    41821 accgctgtcc gcgtgctgat tctaatccaa agtcagcagc gtcagaagtt cctttgacac
    41881 cagctgtcca aatcagtgtg tgagtcggaa tgttgcgtcc gtctttcaat ttgatatgat
    41941 cagcagccac ttctacgatt ggtgcattca ataataattc cacgttgtgc ttacgcaaat
    42001 agcgttcagc ttttgcagca tcatttcgat ccaacatatt caagatcgtc ggcattgcct
    42061 ctacgaccat cagtgtgatt tcgtttggat ctaatttata atctttggct aggcgatttt
    42121 tccagtcgat cagttcccca atcatttcaa taccggtaaa tccagaacca cagacaacga
    42181 aagtcagcat ggctttacgc agttcatcat ctggttccaa tgcagctttt gctactgtct
    42241 tctcaatatg ttcccgaatc cttacagaat cttcaaatga ccaaagggtg aatccgtgtg
    42301 ctttgacacc aggtgtacca aagtcattcg gctctccgcc cattccaagg atcagctgat
    42361 caaaaggata ggaacctaat ttggtcttga cgatttttcg ttctttatcg ataccgatta
    42421 cagtgtctgt gacaagttgt acattctttt gacgagcaaa caaatgctgc agatcgtatt
    42481 ggattgcttc tggttctaca cgtccgccag ctacttcatg caactcggtc atcatagtat
    42541 ggtacgaatg acgatcgatc agagtaattg ctacatcttc ttttttcagt ttctttgcca
    42601 agtattttgt agctgaaaca ccggcatatc ctgctcctac aacgacaata ttttgctttg
    42661 ccattgatca tccgctcctt atagac
//
LOCUS       DACESX010000008        41248 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.008, whole genome shotgun sequence.
ACCESSION   DACESX010000008 DACESX010000000
VERSION     DACESX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.008"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            60..1083
                     /locus_tag="IXF88_000368"
                     /pseudo
     CDS             60..1083
                     /locus_tag="IXF88_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1426..1682
                     /locus_tag="IXF88_000369"
                     /pseudo
     CDS             1426..1682
                     /locus_tag="IXF88_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2069..2296
                     /locus_tag="IXF88_000370"
     CDS             2069..2296
                     /locus_tag="IXF88_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1507128.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2312..3478
                     /locus_tag="IXF88_000371"
     CDS             2312..3478
                     /locus_tag="IXF88_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1507129.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGNRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3713..4756
                     /gene="hrcA"
                     /locus_tag="IXF88_000372"
     CDS             3713..4756
                     /gene="hrcA"
                     /locus_tag="IXF88_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1507130.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4850..5413
                     /gene="grpE"
                     /locus_tag="IXF88_000373"
     CDS             4850..5413
                     /gene="grpE"
                     /locus_tag="IXF88_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1507131.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5464..7293
                     /gene="dnaK"
                     /locus_tag="IXF88_000374"
     CDS             5464..7293
                     /gene="dnaK"
                     /locus_tag="IXF88_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1507132.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7444..8610
                     /gene="dnaJ"
                     /locus_tag="IXF88_000375"
     CDS             7444..8610
                     /gene="dnaJ"
                     /locus_tag="IXF88_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1507133.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(8978..9715)
                     /locus_tag="IXF88_000376"
     CDS             complement(8978..9715)
                     /locus_tag="IXF88_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507134.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9717..11219)
                     /locus_tag="IXF88_000377"
     CDS             complement(9717..11219)
                     /locus_tag="IXF88_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1507135.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11478..13472
                     /gene="uvrB"
                     /locus_tag="IXF88_000378"
     CDS             11478..13472
                     /gene="uvrB"
                     /locus_tag="IXF88_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1507136.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            13482..16298
                     /gene="uvrA"
                     /locus_tag="IXF88_000379"
     CDS             13482..16298
                     /gene="uvrA"
                     /locus_tag="IXF88_000379"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1507137.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16561..17826
                     /locus_tag="IXF88_000380"
     CDS             16561..17826
                     /locus_tag="IXF88_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1507138.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            17830..18561
                     /locus_tag="IXF88_000381"
     CDS             17830..18561
                     /locus_tag="IXF88_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1507139.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            18696..19580
                     /gene="rapZ"
                     /locus_tag="IXF88_000382"
     CDS             18696..19580
                     /gene="rapZ"
                     /locus_tag="IXF88_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1507140.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            19577..20575
                     /locus_tag="IXF88_000383"
     CDS             19577..20575
                     /locus_tag="IXF88_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1507141.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            20609..21544
                     /gene="whiA"
                     /locus_tag="IXF88_000384"
     CDS             20609..21544
                     /gene="whiA"
                     /locus_tag="IXF88_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1507142.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            22112..22768
                     /locus_tag="IXF88_000385"
     CDS             22112..22768
                     /locus_tag="IXF88_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507143.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            22947..23512
                     /locus_tag="IXF88_000386"
                     /pseudo
     CDS             22947..23512
                     /locus_tag="IXF88_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24211..24732)
                     /locus_tag="IXF88_000387"
     CDS             complement(24211..24732)
                     /locus_tag="IXF88_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1507144.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            24930..28610
                     /gene="nifJ"
                     /locus_tag="IXF88_000388"
     CDS             24930..28610
                     /gene="nifJ"
                     /locus_tag="IXF88_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1507145.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            28799..29686
                     /locus_tag="IXF88_000389"
     CDS             28799..29686
                     /locus_tag="IXF88_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1507146.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            29683..30852
                     /locus_tag="IXF88_000390"
     CDS             29683..30852
                     /locus_tag="IXF88_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507147.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            30876..32231
                     /locus_tag="IXF88_000391"
     CDS             30876..32231
                     /locus_tag="IXF88_000391"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1507148.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(32303..34108)
                     /gene="glmS"
                     /locus_tag="IXF88_000392"
     CDS             complement(32303..34108)
                     /gene="glmS"
                     /locus_tag="IXF88_000392"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1507149.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            34518..34808
                     /locus_tag="IXF88_000393"
     CDS             34518..34808
                     /locus_tag="IXF88_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507150.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            34821..35846
                     /gene="sppA"
                     /locus_tag="IXF88_000394"
     CDS             34821..35846
                     /gene="sppA"
                     /locus_tag="IXF88_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1507151.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            35849..36508
                     /locus_tag="IXF88_000395"
     CDS             35849..36508
                     /locus_tag="IXF88_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1507152.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            36665..37597
                     /locus_tag="IXF88_000396"
     CDS             36665..37597
                     /locus_tag="IXF88_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507153.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            37751..39040
                     /locus_tag="IXF88_000397"
     CDS             37751..39040
                     /locus_tag="IXF88_000397"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1507154.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            39287..40537
                     /gene="clpX"
                     /locus_tag="IXF88_000398"
     CDS             39287..40537
                     /gene="clpX"
                     /locus_tag="IXF88_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1507155.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            40620..41207
                     /locus_tag="IXF88_000399"
     CDS             40620..41207
                     /locus_tag="IXF88_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1507156.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT        13677 a         7057 c         8968 g        11546 t
ORIGIN      
        1 tttcgatttt tcaagttatc taatgcaatt atattgatat cattaggagc tgcattgtta
       61 tgaatccaac attgattact aagaaagaat tactaaaaaa attagatatt tctactggtg
      121 tattagctaa tttaattaga aatggcatgc caaaagaggg agaaatgttt aatcttgata
      181 agattattac atggcgagaa aattggtcta aaaacatatt aggtgagtta gaagttggta
      241 gagtatatac taacaaagaa atttcagaga agtttaaatg ttccaaacag ggtggaatga
      301 gaagatctca tcaaactaat actcttgtac tgtttagtga tcaaacagga agtaatgttt
      361 ataaagataa gtggttaaat ggtattttac aatataccgg tatggggcta aaaggggatc
      421 aagtattgga taagaatcaa aataaagttt tagccaattc aaaaagtaat tttgtaaaga
      481 ttcatttatt tgaaacattt aaaccaaaag aacacacata tctgggagaa gtatatttag
      541 caggacaaat atatactgtt aatgaaaaag atagttcggg caacagcaga aaagtatata
      601 aatttccatt agctttaata aatcaagagc aattgattga agataaagat atataaccag
      661 gaagaaaatc aaactagaca tattcgtaat ttatctaatg ctaaattaga ggaagaagca
      721 agaaaagtta gtaattataa taaaaaaatg gctgcaaaat atgttaacga agaggagttt
      781 tgcttctcaa aaacaaaaat atataaacgt aatcccttcg tatctgaata tgttaaacga
      841 cttgcaaaag gtatttgtca actttgccaa gaaaaagggc catttataaa agacggtgta
      901 ccttatttac attgtcatca tatagaatat ttgtcgcaag gtggaaaaga tgtgattgaa
      961 aactgtatcg cgttatgtcc caattgtcat gctagaattc atgaacttga gttacctgac
     1021 gataaagaaa aattgctgaa gatcgttgaa aaaagagaaa atagcttttc taaaataccg
     1081 taatttattt ttataggaca aataagctaa tgtgagctat gccttctata taagactaag
     1141 ttctattata agtaacgaag taattctcgt tccttcctgt agtaatattt tttaaataac
     1201 ctataggctt cttcaaaagt acatcctcca gtaaattcta agtactggag gattcttttt
     1261 ctagtgttgc tttactcatg aaaaagtagt tggctacttc atcaaaattt ctgaactttt
     1321 ttaatgtata ataaagagct cgatcattta ctacaaggat gctagctcct acattttcac
     1381 agttttttag caagtaaatt tttctaaaat cacatcagga aatgcatgag attctttaag
     1441 agaggcaatt atagggctat gattacggtt cgtgtaagtg acgccaaaaa agaatggctt
     1501 tcttatatgg ctgaatttta tgggattact ttatctgaac ttctaaaaaa ctattcgatg
     1561 gaacaactag aagatgaata caatcgtcaa acggctgaaa ttgcccataa acattggata
     1621 agaagtggga aaacaacagt attcatggaa gaatccttaa tgaatttggt gggctggaat
     1681 gacctgccgt ttaaaattca cgcccgaaat tcagaagcag ctctgattgg caaatacgtg
     1741 tattggtaga gatagaagat aatcaattcg ttgttatcgc tattcaagta gggcaccgaa
     1801 gaaatgtcta tgattaagag actgtttatt tttacaaaaa aataataatt ggaatctagt
     1861 taaaactctt gaagtgagag atttgactag atttttttat gaataaaata aggacaaaat
     1921 tgtttaggaa gaatcatgag tgtaaaaaaa tcatccaaga agtcgattct tggatttttt
     1981 agtggatttg ttcgccaaat acgtttagta tgcgtatatg agaggttgta agcaaagcgt
     2041 tgtggtctga gcacttaagg aggaatctgt gaaaatagct tttcatattt ttgcagattt
     2101 tgacttaatg ccgaaggtca gctttgcgat taccgtttat cgagaggctg taaaagcacc
     2161 gtgtagctcc gatgacaata agcaagcatt tttagagcta ggtttccttg attctaaata
     2221 tgcggcttat ttcaatgagt tggtgcttga acgcgatttt aagaaggtcg aaaacaggca
     2281 cgacttttcg acgtaaggag aaagaatttc aatgatagaa acaataaatc ccgcagaaaa
     2341 caaggtttct gcgtacattc atatcccatt ttgcgagcat atctgctatt actgtgactt
     2401 taataaagtt tttttagaag ggcagccagt agatgagtat gtggaaatgc tgctgaatga
     2461 aatgagatta atgctggaaa gtcatcctac agcgaatttg gaaacacttt atgtaggagg
     2521 cggaacacca acttctttat ctgctaaaca acttgatcga ctattatcag gtgctagaga
     2581 gatcctgcct tttcaaaatg gtaatgagtt tactgtagaa gcgaatcctg gtgatttgac
     2641 ccgtgaaaaa cttcaagtga tgaaaaacta tggcgtcaat cgtttatcca tgggagtgca
     2701 gacatttgac aatcgcttat tgaaaaaaat tggacgtaaa catactgcag aagatgtgta
     2761 tcaaacaatg caatttttag aagcagaaaa ttttaccaat gtaagcatcg atttgattta
     2821 tgcattaccg ggacaaacac tagaaggaaa tcgggatact ttggaaaaag ctttggcttt
     2881 ggatctgcct cactattctt tgtactcatt gatcctcgaa aacaaaacaa tgttcatgaa
     2941 ctgggtacga caaggaagac tcgaactgcc tgatcaggaa acggaaacca gaatgtttga
     3001 agagacgatt gaagcaatgg aaaagcatgg acgtcaccaa tatgaaataa gtaactttgg
     3061 gctctccgga catgaatctc aacataacct catgtactgg aacaatgacc actattttgg
     3121 gtttggagct ggtgctagcg gttatcttgg agatattcgt taccgcaaca aaggaccaat
     3181 ccaacactat ttacgcccat tacgagcaaa tgaacgcccg gtattggaag aagaaaaatt
     3241 gacacgaaaa aatcaaatag aagaagaaat gttcttagga ctaagaaaaa aaacagggat
     3301 tactaaaaaa catttctatg atcgctatca aaaaacaatt gaatcggtgt acggtgatgt
     3361 gctaagggaa ttagagcaag aagggttact tgttaatgaa gaagaccgtg tttatttgac
     3421 accaagaggg acttttttgg ggaacaatgt gtttgagcgc tttttatttg aaaaatagtt
     3481 tgaagaatat ttgtttttta tcgaaacttg aaaagaaaaa gtttagaaag tttgacaatg
     3541 aggtattgaa gtattgatat aactggattt acatcaatta tttagaatta gcactcttgt
     3601 gtttagagtg ctaaaaaatg gagtctttct cttgacagga gaagggcttc ttgctatatt
     3661 tataagtgaa ttagcactta gaatatatga gtgctaatga gaggtgagga agatgttaac
     3721 aaagcgacaa aacgacattt tacgtctgat catccaacac tatacgagta gtggcgtgcc
     3781 cgttggttcc aaaacattaa tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa
     3841 tgatatgaaa gcattagaag atgaaggatt gctgttgaag actcactctt cttctgggcg
     3901 gattccatct gctttaggct atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt
     3961 agcagaagat gatttgctgc tgattcgtca gtctctaggc agagaatttc atgaaatcaa
     4021 tgagattatt aagcaatctg ctgagatatt atctgagctg acaagctata cgactttttc
     4081 attgggacct gaaataaaag accgccgact gactggcttt cgtatcgtac ccttgaataa
     4141 tcgacagatt attgctatcg tcgtaacaga tatggggaat gtagaaagcc aagtgttcac
     4201 tcttcccgaa tatatgggaa gtcaggaact agaaaaaatg gtacagattg taaacgaccg
     4261 tctagtcgga gatcctctga tgacggtgta ccataaactt cgtacggaga ttccgatgat
     4321 tcttcataaa tatttccaga cgacagaagg gatgtctcag ttatttgaca ccgttcttgg
     4381 tcatgttttt gaagaaaagg tttatgtcag tggacaaatg aatctcttgg attatgagcc
     4441 ccgtcaagat cttgatcagt tcaaatcgat gttttctttt atgaaagact cagacggact
     4501 tacacaaatg atcactccga tggatagcaa tattcatatc aagattggtg ctgaattagg
     4561 caatgattta ttgcagaata tgagtatgat tcaagcaagt tacgagatag atggtcatgg
     4621 aagaggtaca atagccttgt tagggcctac tagtatgcct tattctcgga tgttcggtct
     4681 agtcgatgtt tatcgaagag aactagcagc taaacttgct gattactacc gctcgcttga
     4741 cttgtcaaac tcgtaatgtg aataacgaag ctatttgcag ctgatcgttc tctctttctt
     4801 agtaagagca atcaaacagc tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa
     4861 tcaagaagaa ctagataaag aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga
     4921 agctgcagaa gattcgggta tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt
     4981 aaaagctgag ttagaagaaa tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa
     5041 tatggcaaat cgaggaaaaa atgagcgtga gcaattacaa aaataccgtt cacaagattt
     5101 agccaaaaaa ttacttccat caattgacaa cctggaacgt gcattagcca cagaagtgtc
     5161 agatgatcaa ggggcaggtt tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa
     5221 cgcactggaa gaagaaggaa ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa
     5281 tcttcatcaa gcagtacaaa ctgttccagc gactgaagat acaccagccg atacgattgt
     5341 agaagtacta caagaaggct acaaattaca tgatcgcgtg ttaagaccaa ctatggtcat
     5401 tgttgcacaa taattgtgcg gatataaaac ttacactttc aattaaagaa ggagacgtta
     5461 attatgagta aaattatcgg tattgactta ggtacgacta actctgcagt agcagtattg
     5521 gaaggaaacg aagcaaaaat catcgctaac ccagaaggaa accgtacaac accttctgtt
     5581 gtatcattca aaaatggaga aatccaagta ggggaagttg caaaacgtca agctgtgaca
     5641 aacccaaata cgatttcatc aatcaaacgc catatcggtg aagcaggtta caaagtagat
     5701 gtagaaggta aatcatatac accacaagaa atctcagcaa tgatccttca atatatcaaa
     5761 ggattcgcag aagactattt aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct
     5821 tactttaatg atgcacaacg tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa
     5881 gtagaacgta tcgtcaatga accaactgca gctgctcttg cctatggttt agataaaaca
     5941 gatcgcgacg aaaaaatctt agtattcgac cttggtggcg gtacttttga cgtatctatc
     6001 cttgaactag gagacggtgt ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt
     6061 ggggacgact ttgataacaa aatcatcgac tacatggtag cagaattcaa aaaagaaaac
     6121 agcattgact tgtctcaaga caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa
     6181 gcgaaaaaag atttatctgg cgtatcaagc acacaaatca gcttgccatt tatcacagca
     6241 ggagaatctg gaccattgca tttggaaatg acattgacac gtgcaaaatt tgatgaatta
     6301 acagctgact tagtagaacg tacaaaagta ccagtacgtc aagcgttaaa agatgcaggt
     6361 ctgtcacaat cagaaatcga cgaagttatt ttagtcggtg gatctactcg tatccctgca
     6421 gttgtagaag cagtacgtaa agaaacagga aaagaaccaa acaaatcagt taaccctgat
     6481 gaagtagttg ctatgggtgc tgctatccaa ggtggcgtga tcactggtga tgtgaaagat
     6541 gttgttttac ttgacgttac accattgtca ttaggtatcg aaacaatggg tggagtgttc
     6601 actaaattga ttgaccgcaa tacaacgatt ccaacaagta aatcacaagt attctcaact
     6661 gcggcagata atcaaccagc agtagacatc catgtattac aaggtgaacg cccaatggct
     6721 gctgacaaca aaactctagg aagattccaa ttgacagata ttccagcagc tcctcgtggt
     6781 attccacaaa tcgaagtaac attcgatatt gataaaaacg ggattgtaaa tgtatctgcg
     6841 aaagatctag gtacacaaaa agaacaaaaa atcacgatca aatcttcttc aggtctaaca
     6901 gacgaagaaa tcgaacgtat ggtgaaagat gcagaagcaa acgctgaagc agataaagct
     6961 cgtaaagagg aagttgacct acgtaatgat gtagacgcat tgctattctc tgtggacaaa
     7021 acattgaaag aattagaagg caaagtggac gaagaagaag tgaaaaaagc agaaacagct
     7081 cgtgacgaat taaaagccgc tgttgaagca aacaacatcg aggaaatgaa aactaaacgc
     7141 gatgcattga acgaaatcgt tcaaaacttg actgttaaac tatacgaaca agctgcacaa
     7201 caacaagctc aagaaaatcc agaagcagct caaggcggag cagatgatgt cgttgatgct
     7261 gattttgaag aagtcgatgg cgacgataaa taatcagttc actgaatttc gagagaaggc
     7321 caaagttgct actttggctt tttcttttga aaaatagttt gaagtatcgg tttttgtttc
     7381 aaacaaaata tgatatgata acaagcgacg tgaaaatgat gagaaccaat aggaggaaag
     7441 cctatggcaa caaaacgtga ttattatgaa gtcttagggc tctcaaaagg agcatcagaa
     7501 gatgaaataa agaaagctta tcgtaagctt tcgaaaaaat accatcctga catcaataaa
     7561 gaacctgatg cggaagaaaa attcaaagaa gtatcagaag catatgagat tttaagtgac
     7621 ccgcaaaaac gcgctgcata cgaccaatac gggcatgcag gcacagatcc aaattacggt
     7681 gctggtggag ctggtggcgg atttggtgga ttcggcggct tctctggtgg cggcttcggt
     7741 ggttttgaag atatcttcga ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat
     7801 gcgcctcgtc aaggtgctga tttgcaatat acggtcgact tgtcttttga agaagcgatt
     7861 tttggtgtag aaaaagaaat caagtataat cgggaagaaa tctgtcatac ttgtggtgga
     7921 aacggtgcaa aaccaggcac acaaccaact acttgtcata aatgtcatgg tgcaggtacg
     7981 atcaatgtag aacgccaaac gccacttgga cgagtgatga gccgtcaaac ttgtgatgtt
     8041 tgtcatggca cagggaaaga aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat
     8101 gagaagaaag cacattcagt taaagtgaat gtaccagctg gtgtagaaga aggacaacaa
     8161 atgcgtttag ctggtcaagg agaagctgga gaaaacggcg gaccatttgg tgatttgtat
     8221 gtggtttttc gtgtagaaga aagtgatatc tttgatcgtg aaggttctga aatttactat
     8281 gaattgcctt taagttttgt acaagcagct ttgggggatg aagtccaagt accaactgtc
     8341 catggagatg tgaaattgaa gatccctgct ggaacacaga caggtacgaa cttccgttta
     8401 cgtggaaaag gcgctccgaa gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta
     8461 aaactgatca caccgaaaaa tctaaatgaa caacaaagag aagcattacg tgcattcgct
     8521 gaagctggcg gcatcaaagt tgaagaacag caagaagacg gtttcttcga taagtttaaa
     8581 gatgcttttg gcgggaaaaa gcggaagtag tttttctagt aaaaatataa agtagacttg
     8641 cccaaatttt ctatttctag taaaaacgct aagtcattaa taattaaaaa ccagacgaac
     8701 aatcatacgt tcgcctggtt tttttgtagt tgtcatgttt gtaccaacaa aaaagccagc
     8761 tttagaataa gataatcatc atagtttctg aaggtggtat cgactatgta cttgatatcg
     8821 tcaatgaacc gatggtactt tcttatgcga tggaacaggt agagcgattg cttgtttgtg
     8881 caggagaaca agcgatgcgg ctggtacagt taagtgaaaa aacaagaaac taaaaatgcc
     8941 cggtcgtcat gaagcgaccg ggcatttttg gtttttatta aatatttaat actttattca
     9001 agaaatcttt tgtccgttca ttttgcggat tttcaaagat ttgttgaggc gttccgtctt
     9061 ctaaaaagtt tccgtcgtca atgaacatga cacgattggc cacttcttta gcgaagccca
     9121 tttcgtgcgt aacgataacc atcgtcatac cttgttctgc taactttttc atgacgttca
     9181 agacatcacc gaccatttcg gggtcgagtg cagatgtagg ctcatcaaaa agcatgatgt
     9241 cagggttcat cgctagagca cgagcaatag ccacacgctg cttttgtcca ccggaaagca
     9301 tttcaggata catatctttt ttatcttcta gaccaactgt ctgcagcaat ttgattgctt
     9361 ttttctctgc ttcttctcta gattctttct tcaggtccat cggtgctaaa atgatgtttt
     9421 ccaaaataga aagatgaggg aataggttaa agtgctggaa taccattccg atatgttgtc
     9481 tcaccaagtt gatatctgtg cttttatcca tcaagtgggc gccgtcaata ataatttcac
     9541 cgtcagaagg ttcttctaaa cgattcaagc aacgcaaaaa tgtacttttt cctgaacctg
     9601 aagggccaat gatacagacg acatctcctt cgttgatgga cacattgatg tcgtttaata
     9661 ctgtgttatc gccgtatttt ttgactaaat gctcaactaa gattttttct gccatcttac
     9721 ttcaccttct tttctaaaac ttttgctaat tttgtcaatg ctgtgatcaa aatcaagtac
     9781 atgatcgcaa tgatcaagta aacatacgta ctttgtgttg tacgtgcaac aatgatctta
     9841 ccagtttgca gcaattcgac tacaccgatt gcggaaatga tcgttgtgtc ttttaatgag
     9901 ataacgaact gattgacgaa tgatgggatc atgatgcgta tcgcttgcgg caaaatgatt
     9961 ttttgcattg tccgattata agaaagacct aaacttctgg aagcttccat ttgtccgaca
    10021 ggaacggcgt tgatcccgcc tcgaacaatc tctgcgatat aagcacttgc gttaagagtc
    10081 aaggtgataa caccagcaag gaagtcagga atcgtaaatc caacgatacc aggcaaccca
    10141 aagaaaataa agaacgcaag aaccatcatt ggaattcctc ggatgatatc gacataaata
    10201 ctagcaattg tgcgaagagc gcgtattggt gctacactga ataatccaaa aatcacaccg
    10261 attactatag ccaaagcaaa ggagatcaaa gttaggagaa ttgttctcca aagtcctttc
    10321 aacaatgctt gatagttgtt ttggataagt ccaagaaatg ttgattcatc agcagtggaa
    10381 gactcactgg aagtttctga accatccgct acatattctt taataatctt atcgtattcg
    10441 cctgttcttt ttaattcttt caatccttca ttgaacatat taagaagctc aggattttgt
    10501 ccttttttaa cggcaaatgc atattttccg ccagattctt ttttgatcgg tgtttccaat
    10561 tcttgtcctt gtttgatcgc ataaccgatt accggataat catccatcat tgcatcgatt
    10621 gcgccaactt ttaacccgtc gtacaattga tcagcagcat caaaatattt gatagaatag
    10681 ccatattcag attcatggtc gcttaaaaat tcagcacttt cggtaccaac ttttgctccg
    10741 actgtttttc ctttcaaatc ttcataagac ttgatcgaag tatctccttt tttgacagct
    10801 aattggatcc cgctatcaaa gtaagaatca gaaaaatcat agcttttctt tcgttcatct
    10861 gttacagtca ttccggcaat cattccgtct gcttgtcctg attccaaagc ttgcaaagca
    10921 gcagagaaac cacggtagtc cattcggatg ttaaatcctt gcagtttggc gatgttttcc
    10981 atcaatttta catcgatgcc ttcgtactcg ccctttgcgt tttgaaattc aaatggagca
    11041 aaagcagagt cactggcaat tacgtatacg tcttttttgg gctctatttt ttgcattgtc
    11101 tcgcttgtgc ttgactctgc cgttgtagtg ttgtcagctt gtgcaacatg tccaaacgca
    11161 agtgtcacaa gtcctgtcat taagaaaaca aatgagtgaa ataaatgctt tcttttcata
    11221 atcaacctcc taaatttcca atattttatt attataggcg aaaagtgctg aatcagacaa
    11281 gactgtaagc tcgaaaaaat gaaaaaaatg cgagtttcta acattttatg ttataaaacg
    11341 taacatgcat aataagataa aaaagtggcg aaagtatgtt cggtgtggta taatttttta
    11401 gtaaggaatg cctttgcttt ccttttcttt ttattggtcg taaaatgaaa aggaatgaat
    11461 gaaaaggagg acgaagaatg attgagagag atacctcccg acactttgag cttgtctcga
    11521 aataccaacc ggctggtgat cagcctgaag cgattaacca attagtggat ggcgtagtcg
    11581 gcgggaaaaa agcacaaatc ttattaggtg cgactggaac agggaaaaca tatacgattt
    11641 ccaatcttat tgagaaagtg aataaaccaa cactaattat tgcccataac aaaacattgg
    11701 ctggtcagct gtacggggaa ttcaaggaat ttttccctaa caatgctgtt gaatattttg
    11761 tttcttatta tgattattat cagcctgaag cttatgtgcc ttcaagcgat acgtatatcg
    11821 aaaaagattc aagtgtaaat gatgagatcg acaaattacg acattcggct actagttcat
    11881 tattagaaag aaatgatgtg atcgtcattg catccgtttc atgtattttt ggattaggtt
    11941 cgccttttga gtaccaaaaa caagtagttt cgattcgaca gggagcagaa ctagatcgca
    12001 atcagttgat tcgtgactta gtcagtatcc aattcgaaag aaatgatata gattttcagc
    12061 gcggtcgttt tcgagtgcgc ggtgatgtcg tggagatttt tccggcttca agagatgaac
    12121 gtgccttacg tgtggagttt tttggagatg aggtcgaacg tatccgagaa gtcaatgcac
    12181 tgacaggtga agttttagga gaaacggaac acgtggcaat cttcccggcg actcactttg
    12241 tgacgaatga tgaacatatg gagcatgccg tagcgaatat caaagcggaa ttagaacagc
    12301 gtttaactgt cttgcgaaat gaaaacaaac ttctggaagc tcaacgttta gaacagcgaa
    12361 ccaattacga tattgaaatg atgttagaaa tgggctacac gtcgggaatt gaaaattatt
    12421 ctcggcatat ggacggacgt aaagaaggtg aaccgcctta tacgctattg gatttctttc
    12481 ctgaagattt tttgatcgtg gcggacgaat cgcacgtaac catgcctcaa gtcagaggaa
    12541 tgtataatgg tgaccgagcg agaaaacaga tgctagtaga ttacggtttc cgtttgccat
    12601 ctgctttaga caatcggcca cttcggttag aagagtttga aaaacatgtc aatcagatca
    12661 tttatgtctc tgcgacgcca ggaccatatg aacatgaaca aacagatacc gttatccaac
    12721 aaatcatccg tccgacaggg ctgcttgacc cagtgatcga agttcgtcca atcatgggac
    12781 aaatcgatga tctagtaggc gaaataaatg aacgagtcga aaaagaccag cgggtgtttg
    12841 taacgacatt gacgaaaaaa atggcagaag acctcacaga ttatttcaaa gaattaggga
    12901 tcaaagtcaa atatttgcat agtgatatca aaacgctgga acgaacagaa atcattcgag
    12961 atcttcgatt aggtgaattt gatgtgttag ttggaatcaa tttgcttcgt gaaggattag
    13021 acgtacctga agtttccctt gttgctatct tggatgcgga taaagaaggc tttttacgta
    13081 gtgaacgttc cttagtccaa acgattggac gagctgctcg gaatgaagaa ggtaaagtaa
    13141 tcatgtacgc agacaaagta acagattcta tgcgtctagc gatggatgaa acatcaagac
    13201 gccgtacgat tcagcagaag tacaatgaag aacatgggat cgttccaaaa acgatcatta
    13261 aagaaatccg agatttgatt tctattacga aagaatcgga agatgataca aaagaagcgg
    13321 tacaagtctc gtatgaagaa atgacgaaag aagaaaaaga tacgttattg atgaaattag
    13381 aaaaagaaat gaaaaatgcg gcgaaagcat tggattttga aacagccgct aatgtgcgag
    13441 atatgatttt agaattaaaa gcttcaaagt aggaggacaa catggctaac gataaaatta
    13501 cgattcacgg tgcacgtgcc cataacttga aaaatataga tgtaacgatt ccacgagata
    13561 aattcgtggt cgttacaggt ttgtccggtt ctggaaaaag ttccctagca tttgatacgc
    13621 tgtacgctga agggcagcgg agatacgtag agagcctttc tgcttatgct cgtcagtttt
    13681 taggacaaat ggataagccg gacgtagata gcatcgacgg attgagtccg gcgatttcta
    13741 ttgatcagaa aacaacaagt aaaaatcctc ggtcgacagt aggtactgtg acggaaatca
    13801 atgactattt acgcttgttg tttgcacgtg taggacatcc gatttgtccc aacgatcata
    13861 tcgagattac cagtcaatca gtagatcaaa tggcagatca agtaatggct ttgcctgagc
    13921 gcacaaagat ccaagtgttg gcaccgatcg tggtaaagaa aaaaggacaa cataaaaaaa
    13981 tattcgaacg aatccaaaaa gagggatacg tccgcgttcg agtcgatggt gagatctatg
    14041 atttaagtga agtgcctgag ctggagaaaa acaaaaaaca tgatatcgcg atcgtcatcg
    14101 accggatcat tgtgaaagaa gggattcgtt ctcgtctatt tgattcttta gaggcggctc
    14161 tgcgattggc agacggctat gctctggttg acgtgatcgg tgaagaagaa atgttgttta
    14221 gcgaacacta tgcctgtcct tattgcggat ttactgttgg cgaactagaa ccaagactgt
    14281 tctcattcaa tgcgcctttt ggagcttgtc cagactgtga tggtttaggt gtcaaattag
    14341 aagtcgatca agatttagtg attccagata gaacgaagac acttagtcaa ggtgctatcg
    14401 ttccatggaa tccaatcagc tctcagtatt atcctcaaat gttagcgcag gcttgtgaaa
    14461 gtttcggtat cgatttagat acaccattcg aagaattacc ggaagagcat caggatatca
    14521 tcttgaatgg gacagatgat cttttccatt tccattatga aaatgatttt ggtggcgttc
    14581 gcgatgtaga aacgactttc gaaggggtat tgaagaatat cgagcgtcgt tatcatgata
    14641 cgaacagtga tttcacgcgt gagcaaatgc gtctttatat gacagaatta acttgtcaga
    14701 catgtaaagg ttatcgattg aatccccaag ctctatctgt tcaaatcaat ggaacgaata
    14761 tcgggcaaac aaatgagttg tctattcaaa aagcagtaga tttcttctca aacttaacgt
    14821 tatctgaaca agagaaagta attgccaaac cgattttaaa agaagtaaat gacagacttt
    14881 cattcttgga aaatgtggga ctagactatc taactttgag tcgcgcttcc ggaacattat
    14941 ctgggggaga agcgcagcga atcagattgg ctacacaaat tggatcgaat ctttctggag
    15001 ttttgtatat tttagatgaa ccatcgatcg gattgcatca acgcgacaac gatcgactga
    15061 ttggctcatt aaagaaaatg cgtgatcttg gcaacaccct cgttgtagtc gaacatgatg
    15121 aagatacgat gtgggcagca gattatctga ttgacgtcgg acctggggcc ggagaacaag
    15181 gcggacaaat cgtggctgca ggcactcctg aagaagtagc taatgatgaa aattctttga
    15241 cagggaaata tctttcaggg aaaaaatcaa tccctgtacc aaaagaacgc cgtaaaggta
    15301 atgggaaagc aatcaagatc actggtgctt cagaaaacaa tctgaaaaat attagtgttg
    15361 aatttccatt aggagaattt gtagcagtca caggtgtgtc tggttctgga aagagtacat
    15421 tggtcaatag tatcttaaag aaatcgttag cgcaaaaatt aaataagaat tctgctaagc
    15481 caggtaaatt caagacaatt tccggctacg aaagtatcga aaagatcatc gatatcgatc
    15541 aaagcccaat cggccggacg ccgagaagta atccagcgac ttatacaagt gtatttgatg
    15601 atatccgtgg gttatttgct caaacgaacg aggcaaaaat gcggggttat aagaaagggc
    15661 gttttagttt caacgtaaaa ggcggtcgtt gtgaagcttg tcgcggggat ggaattatta
    15721 agatcgaaat gcactttttg cctgatgtct atgttccttg tgaagtatgt catggcaaac
    15781 gatataactc tgaaacatta gaagtgcatt acaaaggaaa aagcattgct gatattttgg
    15841 aaatgacagt agaagatgct gtagaattct tcaagcacat tccaaagatt catcgcaaac
    15901 tgcaaacgat tgttgatgtt ggcttaggtt atgtgacgat ggggcaacca gcaacgacat
    15961 tgtccggtgg tgaggcacaa cggatgaaac ttgccagtga attgcacaaa atctctaatg
    16021 gaaagaattt ctatatacta gatgaaccaa cgacaggact tcatagcgat gacatcgccc
    16081 gcttgttgca tgtattacaa agattagtag atgctggtaa cacagtttta gtgattgaac
    16141 acaatctaga tgtaatcaaa acagcagatt atatcattga tttaggacca gaaggtggag
    16201 aaggtggagg aacgatcctt gcgactggaa caccagaaga aatcattaac gtaaaagaaa
    16261 gttatacagg tcactatttg aaaaaaataa tggtataaag aggatagata gctgagatag
    16321 ttaaaaaaac ataaaaaaat gaatttttgc ttgttttatt aatatcagtg ttctagaatc
    16381 attacatggt agttctttct acactgattg gtatagacaa aaacttccta taagttcaat
    16441 ccgttcaaaa aagaattgag caatcggagg agtttccttt gattgctttt ttctttatgg
    16501 tatgatattc cttgtgtagt tggaaaagaa aaacaagaga ttatgagtaa gtgagagata
    16561 atgatgaaca gtattgaaaa tgaagaattt atccctatat tattaggaag cgacatgaac
    16621 gtttatggta tggcacgttc ttttaatgaa gcatacggca aaatctgcca agcatatgct
    16681 tcggatcagt tagcccctac ccgctacagt aaaattgtca atgtagaagt tattccagga
    16741 tttgataaag accctgtgtt tatcgagacg atgcttcgtt tggcaaaaga aagatattct
    16801 gataagagta aaaaatatct tttgattgct tgtggtgatg gatatgcaga attgatctcc
    16861 caacataaac aagagctgtc agaatatttt atctgtccat atattgatta ttctttattt
    16921 gaaagactga tcaataaagt aagcttttat gaagtttgtg aagaatatga tcttccgtat
    16981 cctaaaacat tgatcgtcag agaagaaatg ttagtaaatg gacatttgga acaggaattg
    17041 ccttttgaat tcccagttgc tttgaaacct gcaaacagtg tggagtatct ttctgtccaa
    17101 tttgaaggaa gaaaaaaagc ttttatttta gagactagag aagagttcga tttaatttta
    17161 ggccggatat acgaagccgg ttataaaagt gaaatgattg tacaagattt tatcccgggt
    17221 gatgatagta atatgcgtgt gctaaatgcg tatgtagatg aagatcatca agttcggatg
    17281 atgtgtcttg gtcatccact gttagaagat cctacaccag catcgattgg taattacgtt
    17341 gtgatcatgc cagattataa cgaaaaaatc tatcaaacca ttaaggcatt tttggaaaaa
    17401 atcgaatata caggctttgc caatttcgat atgaagtatg atcctagaga tggagaatat
    17461 aaattatttg aaatcaattt gagacaaggt cgtagcagtt tctttgtgac attgaacggc
    17521 ttgaatctgg ctcgtttcgt cacagaagat cgagtgttta acaaaccctt tgttgaaacg
    17581 acctatggaa cgaatcaatc agataaagca agattatgga tgggtgtgcc caaaaaaatc
    17641 tttttagaat atgctcggga aaatgaagac aaaaaactag ctgagcaaat gatcaaagaa
    17701 aatcgttacg gtactactgt gttttatgaa aaagaccggt caatcaagcg ctggttgttg
    17761 atgaaatata tgttccataa ctatattcca agattcaaaa agtattttca tgtgaaagaa
    17821 ggctagttca tggagaattt tttcagtatt ttaggcggaa tgggcacgat ggcgacagaa
    17881 agttttgttc gcttgatcaa ccaccgaacg aaagctacaa aagatcaaga atatttgaat
    17941 tatgtcttat tcaatcatgc aacagttcct gatcgtacag cttatatttt agatcgatca
    18001 gaagaaaatc caatgccgtt tctgctggat gatattgaga aacaaaattt attgcagccg
    18061 aacttcattg tgttgacttg taacaccgca cactactttt ttgaagaatt acaagcggca
    18121 acggatattc ctatccttca tatgccaaga gaagcagcaa atgaacttgt ccgtcagcat
    18181 acgacaggga gagtagcgat tctaggaaca gaaggaagta tgaaagctgg aatctatgaa
    18241 cgcgaagtca aaaatcttgg attcgaaaca gtgattcctg atactgttct tcaagaaaaa
    18301 atcaattatc tgatctatca tgagatcaag gaatcggatt atttgaatca agagctatat
    18361 tacgaaatct tagaagaagc ggtagagcgc ctaaactgtg aaaaagttat cttaggttgc
    18421 acagaattgt cattgatgca tgaatttgct gaagataacc attatccagt gatcgatgcc
    18481 caatctattt tagcagatcg gacgattgaa cgagcgctag ctgaaagaag caaggcattg
    18541 gatacagcat cggaaaagta aaaaaattgg atttggggca aatatcctaa aagatatttg
    18601 gtccaaatct ttttgtaaaa gaaaaagaaa actggtaatg atacgcccgg aatgatataa
    18661 ttttactatg cacaatgtga gaggagttcg taattatgac agataatttg caattagtca
    18721 tcataacagg tatgagcggt gcagggaaaa cagtagctat ccaaagtttt gaagatatgg
    18781 gctatttttg tatcgataat atgccgcctc gattgattcc aaaattctgg gaattaatca
    18841 aagaatcagg caaagtcaca aaaatggctt taggagtgga tttacgttct cgttccttct
    18901 ttgaagaaat ccaaaacatg ctgatcgaaa tcgaaaatac gaacttcatc gacacttcta
    18961 tcttattttt agatgcttca gacgaagaac tagtggctcg ctacaaagaa acaagacgag
    19021 cacatccaat ggcgatggac ggtttgatca ctgaagggat acgtaaagaa cgggcgatac
    19081 tagacgactt gaaagcccaa gcttccgtga tcattgatac gacgacattg acaccacgac
    19141 aattacgtga aaaaatcaat gagtcattca aatcaaaggg agaccaagga tttcggatcg
    19201 aatttgtatc atttggtttt aaatacgggt tgccgatcga ttcggatatt gtgatggatg
    19261 tgcgtttttt gcccaaccct cattatatcc cggaactaag accattgacc ggaaaagatc
    19321 agccagtcta tgattatgtg atgtcctttg atgaaacgca atcattctac cataaatttt
    19381 tggatcttct ggaaactgta ttgcctggat acattaaaga aggaaaaagc agcttgacag
    19441 ttgcaatcgg ctgtacaggc ggtcaacacc gttctgtggc attgactgaa cgtttggcaa
    19501 atgctttagg gaaaaactat aaagtcaatg tgacacatcg ggacaaggat aaaaggaaag
    19561 agacggtgaa ccggtcgtga aaatgaaaac ctaccggata agaaaaccaa agatcgtagt
    19621 ggtcggcggt ggcactggtc tgccagttat cctgaaaagt ttgcgaaatc aaggagcaga
    19681 tatcacagca gtagtgactg ttgcagatga tgggggaagc agcggtgcaa tcagagaatc
    19741 gatcgctatg gctcctcctg gtgacttgag aaatgtctta gtggcgttat cggatatgcc
    19801 acaattctat gaagatattt tccaataccg tttcaaaaag gaagatcagt ttttagccaa
    19861 tcatacgatc ggtaatctga tcattgccgc agtttccgag atgcggggaa gcacttatga
    19921 ggcgattcaa ttattagcaa aaatgatgca tgtagatgga catgtttatc cttcttcaga
    19981 aactcctttg accttgcatg ctgtctttaa agacggaacg actgcagtag gagaatctaa
    20041 aatcgcgatc gaccgtaaaa ccattgaccg tgtgtatgtc tgcaacacaa atgataaaag
    20101 tgaagccaaa gcggctcgaa aagtcgtttc agcaattatg gacgccgaca tggtcgtttt
    20161 gggacccgga agtttattca ccagtatcct tccaaattta gtgataccag aaatcggcga
    20221 agcaatgctc aagacaactg ctgaaaccgt ctatatctgt aatatcatga cccaaaaagg
    20281 ggaaacggaa catttcacag atgccgatca tatacgtgtt ttacacaagc atttgaatca
    20341 gccttttatc gatacagtat tggtcaatac ggaaaaagtg cctgaagatt atatggaccc
    20401 agagatctat gatgagtatc tagtacaagt cgcacacgac ttcaacggat tgagaaatga
    20461 aggatgtcga gtgatttcca ccgacttttt ggaattgaaa gatggtggtg tcttccacga
    20521 tggagacaaa gtagtagaag aattgttccg tttagttttc ggttcaaaat attaagcaat
    20581 ttattgttgg agaaaggagg agaataggat gtcatttgct gcagatgtga agaaagaact
    20641 aactggtttg gaagttcatc gagaacatgc gaaagcggaa ttagctgctt tgctgcgaat
    20701 gaacggctct ttaggtattg tgaaccagca gtttattttg aacgtccaaa cagaaaatgc
    20761 tgcaattgcc agacgtatct attctctttt gaaagatcat tatcaggtac gaagtgagct
    20821 actcgttcgt aaaaaaatga aattaaaaaa gaacaatgtc tatatcgtcc ggttgaagca
    20881 aggcacaaaa gatgtgttga tggatcttga tatcatggat ggtatgatgt tcaatggaca
    20941 cgtttcggac gagattatgg gaaattcgca aaaaatgcgt tcttacttac gtggtgcttt
    21001 tatggcctca ggttcagtca ataatccgga aacgagccgt tatcatttgg aaatattttc
    21061 tatttatgaa cagcataacc aagacatttg cgatatgtta aattattacg gtctacatgc
    21121 tcgaacgctt gaacggcgca atggctatat ttcttattta aaaggagcag aaaaaattgc
    21181 tgatttctta acattgattg gtgcaaccaa ctcaatgctc cgatttgaag atgttcgaat
    21241 cgtacgggat atgagaaatt cggtcaatcg tctagtcaat tgtgagacag caaatctgaa
    21301 caagacgatt gatgcggctt ctaaacaaat cgaaaacatc gaattgatcg aagcaaaggt
    21361 cggtctacat gcccttcctg aaaaacttca ggaaatcgca gaacttcggc ttcaacatcc
    21421 agaggtcagc ttgaaagaat taggggaaat gattccctca ggtgcgatat caaaatcggg
    21481 aattaatcac cgaattcgca aaataaatga atttgcggaa aaactgcgtg aagaagcagt
    21541 ttaatccaaa gaaaaaactg atacatgaag caggagatgc gaatgtatca gtttttttta
    21601 aaagttatac ttgcttttct tccgtcaaac tataattcca tctttactgg agtaacataa
    21661 tttggaaagg cttctattgt ttttaataca atcgtataaa aagcggggag acaactgaat
    21721 gaagatttta gttttgtaca taaatttttg cttaatatag aagtcctgat atagataaga
    21781 tcaagtattt atctataact ggcctgatta attaaaaaac tggaaggaac tgctcgatca
    21841 tccttgagaa ctgctcttct tacttctggt caagattctc gtggttggtg gataaggatg
    21901 gttgataagt tcattggatt agctagtcga aaacaaccag gtgtaattaa tgtagaagtt
    21961 ttaacaaggg gatggagtga tttattcagt gataatcata agttcataaa tagtaattga
    22021 gatgtatggt tggaatacgt tatacctgaa cttgaactac caagatttgg tttagaattt
    22081 gagaatcgat gtttataaag ggagggcaaa aatgcgtttt tttacaactt ttactatcat
    22141 tatgattgca gtacttttta tttttctaga tatagcaaag cgaaatacag catttttatt
    22201 atatcgggtt ttacttcgag caggtctcat aacatttata tcgatcgttg gattttttct
    22261 attcacagtt attgtattta tttggagaac tccagcacca ccattgccag agattacata
    22321 tggagaattt ccttttagac ttgagtatga attgaatgaa gaattacatg tgatagaaga
    22381 cacgttaata gttgaatttg atggatttgg aatgaacgaa ggcataggga gatatcgtag
    22441 atggacaagt cgtttagcca gcggagaaga tttagtgttg ctgttagaag ttagtgataa
    22501 taagcaaata ttttattttc caggacctgc taattattac atgggagata ggttgaatgg
    22561 ttataatcat acttttccta gtgcttcttt cattgaaagg gaaagaggca ttactagaag
    22621 ggatatttta catgacaaag aattattaga acaatttggt ccgcttgatc aaaatacaat
    22681 aaatgaagaa gaattgttaa atcaatataa tataaggcta gtgaattggg aaatatctga
    22741 accgattgtt aacaattttg gtgattagaa aacaattacg ataaatagaa acaagccaaa
    22801 tcatcagcaa gtaaaagctg atgatttggc ctttcttata cgagtagtag gaattcatag
    22861 cgaatattat tacgatcagt agatattttg atattaaagg agtattacga actagtatgt
    22921 gagaatgtag aggtgatttg cgaataatga gaaatttatc aaatagaaac aaaatattaa
    22981 tcattattgt tgttatagct gtgtttcatc tgggaacgaa tgcggtttta agtagaataa
    23041 tattgggtcc taagccacca agacctgcga ttacccgagg ggagtttgat tttagattag
    23101 aatatgaagt agatggggaa aggattgtga tagaagatac aattgttgca ttatttgatg
    23161 gattttctgc agatgcaggt agcatggcat ggtatcgcac gtggcgtttg catttggcga
    23221 gtgacaagag ggcacaacaa ttattgttac atgagttaga tgatggtcga aaaatatatt
    23281 atcattttgg aggagctgga ttttatatgg ggaagttagt agcagtgtaa ctgtaacaag
    23341 gaaagatttg ccaggtcttg gcattcgaaa ctccatattt tatatcgagc gtgggagaga
    23401 tacatctatt gggaacagga aagggttaac tcttgaagag ttatataatg actttggaat
    23461 ccgtcttgtt agttgggaac acaatccacc aatagaaaat cgctttgaat aatcgaaatc
    23521 tattaatcca tttcatcagc cagtgaatgt tgtaatggaa caatttggtt tagtgttcaa
    23581 aaccattgaa atactaggct gtgatcagca aaaatgtatc tgaataaaaa agagagcaac
    23641 tattaaatga gaggagttag gagttgttag ctttatacga atcaaagtgg caaatacaga
    23701 tgcgtatgca tttgtgtttg ccatcttgcg tttggtagac gagataggta taagctaaag
    23761 aaacaacttg tttaaaataa acgacctagc tcaacaaacg accatctaat caactaacag
    23821 catacataaa agcaactatt tttgaagtag aattgaaatc gggataactt aaaaagtgat
    23881 tgggatacta gtgtgaatca ccgtacataa atattactcg ttaagggaat ttgagaaatt
    23941 ttgtatgaaa atttaagaat tagttagtat gcgcaagtca aaaatatgaa taaattattt
    24001 ggtggagata atttgctcag cttgtcataa gaaatgagat aatctggcag atgcaagctt
    24061 ttggacacca tttattcgga ataaggaggc tgggacagaa gcgtttaact ccaagaaata
    24121 agaaggagtt gcttctgctt ccgccgttta taggtgttta gagcgtgaga caaaagtgtt
    24181 ctttactttt gtcctacgct ccttattttt ttattttgtt tggatcaatt cttttaattc
    24241 ctctattgaa atagattcat caaagatttc tttacccaag atgacagtcg gaacaaatcg
    24301 aatatgtgct tgctcagctt cgttgatgac agcttggctc agctgttcgt ctttttgttc
    24361 tgtcagcttt agtttatcta ctgcaaaatc cgctacttct tggagagaaa gatgtttcca
    24421 ctcgtcttga gtatcgaata tttgtttgat ctctttgatc gctttttgac catcgctgat
    24481 tgtgagatac cggtgcatca cgtttcctcg ctgcaagctt tctttttctt tatcgaacaa
    24541 tttgatcact cgctgcaatt gcccgctttg gactgcttct tctaatagct cataagattc
    24601 ttcaaaccat tgtcggcaat acggacaaga taggttgatg aattcgacca tttttttagg
    24661 agcattcgat gaaccataaa ggattccttt ttgtgtattt gttttcgttg catcaatcac
    24721 tgatatatcc atgttaaaac acctctttcg cttatagttt atacatgtct gacaaaaaaa
    24781 ggcaacgatt tagactgttt gtctaaaaat tagatgacca attcaaatag acatatgtga
    24841 aatattttta taaataaaag aaaaaatgga atgttttacg ttataatgga acagataata
    24901 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
    24961 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
    25021 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaatgtat
    25081 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
    25141 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttctcaa gggctgcttt
    25201 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccatgttg
    25261 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
    25321 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
    25381 tagcacctgt tgcccatctt gcttctattg aaggagaagt accttttttg aatttctttg
    25441 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
    25501 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
    25561 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
    25621 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
    25681 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
    25741 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
    25801 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
    25861 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
    25921 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
    25981 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
    26041 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
    26101 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
    26161 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
    26221 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
    26281 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
    26341 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
    26401 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
    26461 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
    26521 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
    26581 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
    26641 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
    26701 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
    26761 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
    26821 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
    26881 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
    26941 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
    27001 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
    27061 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
    27121 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
    27181 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
    27241 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
    27301 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
    27361 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
    27421 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
    27481 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
    27541 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
    27601 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
    27661 aagactggtt ggtacacatg tttgagagcg aaggaacgca gcaaagagca gcaaaactgc
    27721 aagctgcgat cgaaagcgaa cgatcaattg tccctgaact ggaggatata tacaagagaa
    27781 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
    27841 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat attcttgttt
    27901 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
    27961 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
    28021 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaga
    28081 cgataaaggc ttttgaagaa gcagaaaagt atcctggacc ttctttaatc attgcctata
    28141 cgccttgtat cacacatggt ttgcttggtg gaatgaaaga atcgcttaaa gaagcaaaag
    28201 aagctgtttc ttctggctat tggtcattat atcgatataa ccctgcaatg gtcgaaaaaa
    28261 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
    28321 tgaggaaaca agttcgcttt tcctcgctag agaacgcaaa tccaaatctt gcaaaagctt
    28381 tatttgaaaa gactatccaa gacgctaaaa cacgttttta caactatgct gcactgacgg
    28441 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
    28501 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gccagagcag
    28561 aacgacgtaa aaaaatggaa caagctacaa aagaaaaaaa ggaaaaataa cagaaaaaat
    28621 ataatgaata attctctgaa gtttaaaaat atttattgca gagaaaaata aaacgcatac
    28681 ttgcaatttt cctgaaaatt atgcgttttt ctcaagaaaa atacgagcag atcagcctct
    28741 aaaattaaaa catatgatat actagagagg aaatctcaaa agagaagaga ggctttttat
    28801 gtcatttcaa atcggggaac tttttaattt ggactattgg cagcaaatgt tttccggtga
    28861 cttgttctct ctgaattttt tggtgaacat acttgatatt ctggttgtct ggtatcttgt
    28921 atacaaactg atccaactgc tgcgaggcac aaaagcgatt cagcttctga aaggtgtagc
    28981 tatttttatt gttatccgaa tcgtagcgga aatcattggt ctgcacacct tgtcttggct
    29041 gatgaaccaa gtcattacgt acggggtgat agctgctatc gtgatttttc agccggaaat
    29101 ccggagagga ctggaacatc ttggacgcag ttcgcttttc aaacaatcaa aaagcgaaca
    29161 gcaggaagat gagaagatga tcttgtcttt tgacaaagcc atccaatata tgtccaagcg
    29221 aaaaatcggt gcgctaatta ctatcgaacg gcatactgga ttagatgagt atattgagac
    29281 aggaattgca ttagatgcag atatcactgg agagctgctg atcaatatct ttattcctaa
    29341 tacaccgctg catgacggtg cagttattgt aaaagaagga aagatcgcag tagcaagcgc
    29401 ctatcttcct ctatcagaaa gtatgttgat tccaaaagaa ttcgggactc gacaccgggc
    29461 agctgtcggt ataagcgaag tcagcgacgc gattaccatc gttgtctctg aagaaactgg
    29521 ggatgtcagc attacattag ataatgaact gatggctggc ttatcccagc aagaatattt
    29581 ggcaattttg cgtagagaac tgattgctga agagaaaaca aaaaacaaga aaaacgtgat
    29641 tcaatctttc ctagacggta tgactaaggg aggggatcgc aaatgatctc aaaagagaaa
    29701 cggacaagca ttctttacag ttttattgca ttgctgttca gtattgcgct gtacttcaat
    29761 gcaaatggcc agtccgttcc aaatacgctt tctggaaatg aagcgtataa tcaaacagtt
    29821 actggggtgc caatcaagat ttcttatgat tcaaagcgct actatatcca tggatatgaa
    29881 aatgcagtga ctgtcaaact ttccagtgca aaccgagttc agttgaatgc agaggcaaac
    29941 gaagatacac ggatgttccg cgtcacagca gatatcacta agctgggcga aggaacacat
    30001 gaggttccgc taaaaattca aaatttgagc agtgctgtca cagcaaagat cgaaccatcg
    30061 actattacag taacggttga gaaaaaggta ataaaagatt tcgatgtgga aacgttgatt
    30121 ccttcaacgt tcacaccatc tggatatgaa cttgatgata cttccgttag tcctaaaaaa
    30181 gtctctatca ctacaggtga caaaacattg gcagaaatca aacgagtagt agctgatgtg
    30241 gacgcttcga tggtcacaga tgatgggatt aatagtgagg tcccaataca ggcgctgaat
    30301 gcagcagggg aagtattaag tatcgtctct gatccagtgc aagtaacagt gagagctgat
    30361 tcgatcaagc caagcaagtc ggtacgactt tatgggatac agcaaggaac accagctgcc
    30421 ggggtagaaa gctatgattt cagtttttca aaactggaag cagaagtaag cggctctagc
    30481 gatttgcttg catcgatcgg tgacagtatt gctgtaccga tcaatgtctc aggtatcaat
    30541 catcgaacaa ctcgtaaaat tgaaatacca gtagaagagg ggttaagtat caagcccgaa
    30601 tcagttagtg tggagattac acccgtgttg caaacgtcaa gcagtagctc tgcttcgacg
    30661 agcagtcgga cacaagaaaa tacaagtacg agtagaagta tttcatctgc tgattcagga
    30721 acggaaccat cgacaacaac catttcttcc agttcggaat ctgggacaag cagctcgacc
    30781 acaagtacag aagaaacatc agaaacaagc gaatcaacac aaatgagcag aacaaattcc
    30841 tcagaaaatt aagttaaagg agtaatttta caactatggg taaatatttt ggaacagacg
    30901 gcgtacgcgg tgaagccaac aaggaactta cgccagaact tgcatttaag ctgggacggt
    30961 gcggaggtta tgtcctaagc cagcatgaag aagaaggaaa acgcccacgc gtgttagtag
    31021 ggcgggatac aagaatctca ggtcaaatgt tagaagaagc gctgatttca ggacttttat
    31081 cggtgggtat cgaagtgttc cagctaggtg tgatctctac accaggtgtt gcttatctga
    31141 cacgtttgca aaaagcaagc gcaggggtca tgatttctgc ttctcataat ccagcacaag
    31201 ataatggaat caaatttttc ggtgcagatg gttttaaact agtagatgac caagagctag
    31261 aaattgaagc attattagat gctgaagtgg atgaattgcc tcgtccatca gctgaaggat
    31321 tgggaactgt agaagaattt ccagaaggct tattgaaata ttctcaattc cttgtacaaa
    31381 cgatcaacgg tgacctttcc ggtttgacca tttgtgtcga tgcagcaaac ggagcaactt
    31441 caacaagtgt taatcgtttg tttgccgatc ttgaaacaga tttttatacc atgggcacaa
    31501 atccagacgg gttaaatatc aatgatggcg taggctctac ccatccagaa cgtttagctg
    31561 agatggtcgt tgaaaaagga gcagatgctg gactagcttt cgatggcgat ggcgatcgta
    31621 ttattgcggt ggatgaactt ggaaacattg tcgacggtga taaaatcatg tttatctgtg
    31681 ctaaatactt ggcagaaaaa aatcgattga aaaaagatac gatcgttacg actgtcatga
    31741 gtaacttagg attccacaaa gcggtggaag gaatcggttt gaaagatgtc gttacacaag
    31801 ttggtgatcg ttacgttgta gaagaaatgc gcaaaaacga ttataacttt ggcggcgagc
    31861 agtctgggca catgattttc ttagatttta acacaactgg agatgggatg ctttcaggaa
    31921 ttcagttact gaatatcatg aaacagacag gtaagaaatt atcagagctt gcagcagagg
    31981 taacaatcta tccgcaaaaa ttagtgaata tccgagtaag tgataaaaac ggtgcgatgg
    32041 atgttccggc aatcaaaaaa gtgattgaag aggcagaagc tgaaatggga tcagaaggaa
    32101 gaatcctagt tcgtccttcc ggtacagaac cattacttcg agtgatggca gaagcgccta
    32161 ctgatgagaa agttaattac tatgtcgata aaatcgcggc agtagtcaaa gaagaaatcg
    32221 gactggatta agagatattt tcataaaaaa taaaagaaca aggaccagtg tcagatgtct
    32281 gacactggtc cttgttcttt tattattcta ctgtcactga tttcgctaaa ttacgtggtt
    32341 tgtcaacgtc gaatccccgt aatagcgttg cgtaatatgc aagtaattga gtcggtacga
    32401 ctgtggttag agtagttaga taagggtgga cagttggtag caccagctgg tcactttcct
    32461 tttctaatcc ctctgagaca ataactagtg ttttagcgcc acggctttcg acttctttca
    32521 ggtttcctcg agtatgcgca ccagtcactt cttcactgat gattccaatc actggtgttc
    32581 cttcttcgat caaagcaatc gttccatgtt tcaattcgcc agcggcaaaa ccttctgcct
    32641 ggatataact gatttctttc aatttcaaag cagcttcaag agaaacgttg tagtcagcgc
    32701 cacgtccaat gtaaaaggca tttcttgtat cgctgagata tacttctgcc aactcttcta
    32761 aataatcttt ttgggcgatg accgattcca tcgcattagc aactaaactc aattcatgag
    32821 tgatatcgaa tacaagagaa gtttcaaatt cttttttatc accgatagct ttggctaata
    32881 aagttagtac agctatttgt gcagtataag ctttcgttga tgctactgcg atttcgggtc
    32941 cagcatgcaa tagcagtgta aaggatgctt cccgcgaaag agtagaacca gcaacatttg
    33001 taatcgtaag tgaagggtag ccgagacggt tgatttttac cagaacttgg cggctatctg
    33061 ctgtttcgcc actttgactc aagaaaataa agaatggttt ttttgaaaga agaggtgtgt
    33121 tatagccaaa ttcactggct aaatgaacct caacagggat ctgtgtcaat ttttccagag
    33181 tctgtttacc tgctaacccc gcatggtaac tagttccgca agcaacgata tagattcgat
    33241 cgctatcaga caatgtatcg accagctttt gatcaagaga gacacgattg tcttctgtta
    33301 cgtatttttg agcgatacga cgcatgataa taggctgttc gtcgatttct ttcagcatat
    33361 aaaaaggata ggtacctttc tcgatatctg aagaatctac ttgtgcttcg aatgaagaac
    33421 gttctacgat gtcacctgca aaattttgaa taataacttt ttctgatgtg acggtaacca
    33481 tctcttcatc ttcaatctca acgaattcct tcgtatgagc aagcatagcc attgcatcgc
    33541 tggcaatcac attgaatcca tcgccgagtc cgattaatag tggactttta tttttagcga
    33601 cgtaaataac atcaggtgct gtccgatcaa tcaaagcgaa agcgtaagaa cctttgatga
    33661 cccgcaaagc ttttaaaaac gcctcttgcg ttgtagtatc cttcgcgaaa gtttcaatta
    33721 gatgagcgat aatctcagta tcggtctcac caataaagtg gtcatttgct aaataagcaa
    33781 ttttaagttc ttcaaagttt tcgattacgc cattgtgaac gagcacgaag cgtccacttt
    33841 gggaagtatg tggatgggcg ttttcttcag aaggttgccc gtgagttgcc cagcgtgtat
    33901 gaccaatacc tatatttcct gtagtatcaa tatttatttt gtttttcaaa ttttgaattc
    33961 tgccttgtgc tttgaccaga taatcagttg tgccatctga gacaaagata ccagctgaat
    34021 cataaccacg gtattctaat ttttctagac cgtcaatcaa tcctggtgtt acatttttta
    34081 atccaatcat tccaacaatt ccgcacataa aaaacatcct actttctata atgaattcgt
    34141 atgaatgctt ccagctgcag gaaaatcctt ttgttttatc tttgcagata ttttttgtgg
    34201 attttctgta gagtgcagtc cctagggcca tccgccgaat ctttcgataa accctctcct
    34261 cgtcatctgt ttacacagac ctggcgctat cctaaaaggt ataactatcc tcctcttttg
    34321 ttttttatct ggaaacattt aaaatcataa ccttttgcga atgttcttgt caacaaaaaa
    34381 agaataaaaa ataaattggt atatatcaaa tgctttgtca aatgctcttt ttatgtcaaa
    34441 aaataatggg tttttatcta tcatcttttt caggagttcg gtacaataga aagtaatcgg
    34501 atagaggagg agtaaaaatg gattcaaaaa ttgagatcat gacactggga atgttaaaaa
    34561 aacaattgtc caaattcgaa gcatcagctg gtgtatcaga tgatacgaaa atatttttag
    34621 atactggctg ggatagcatc caagaaatcg cacctgatgc cttggaagtt gctgaagctc
    34681 gtgagtttac ggtagaagat gaattgacaa aagagtcgtt ttccgggtat gcgcgggaag
    34741 aaaaagcaga gcggttcgac acttctgagc agtcagaaac tgtgatcgtc ataaaaaact
    34801 tgtattagaa aggagaaaga atgaataaga gacgttgggc agcagttctg attgctgcag
    34861 gattatttgt ggtttcgtta gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa
    34921 ttgagggatt gaacagttgg ttatatgggg atgaagaact agcttcaaat gtgttggaag
    34981 agggagacag tacgcaaaaa atcgtaaaat tatcagtaga tggaacgatt gctgcgggag
    35041 gggcttcagg tttatttgct tctgaaggtt acaatcataa aaattttatg gaacaactga
    35101 aggcgattga agaagatcca acagtcaaag gtatcttttt agaagtaaat tctcctggtg
    35161 gcggtgtcta cgaaagtgcg gaaatcgcta agaaattgga tacgattcgt aaagaacatg
    35221 atatcccaat gtatgtcagc atgaaaaaca tggcagctag cggtggctac tatatttctg
    35281 ctcaagcaga taaaatcttt gcaacagaag aaacagtgac cggctcaatt ggtgtgatca
    35341 tgtcaggatt gaattattca ggacttctag aaaaactggg tgtggaagat acaacagtta
    35401 aaagcggagc gttgaaagat atgggttcag gaacgcgacc agagacaaaa gaagaagaag
    35461 cggtgcttca agcatatatc gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg
    35521 gacggaataa atctgaagaa gaagtcaaaa aaattgctga tggaaggatt tatgatggtg
    35581 tccaagcaaa agaagtaggt ttagtcgatg aattgggatt tcctgaagat gctttaaaag
    35641 ctatgcgaaa ggaacaacag cttgaagatg cggaactagt agaatattct tccacgccat
    35701 ctgggtttgc tagtacatgg ttaggaagta aactagcaga attccaggga ttgaagcctt
    35761 ctgagactag tcagatctta tccgttcttg agaatctagg gacagcggaa tcaccaaaag
    35821 caatgtatta ttacggaggt gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca
    35881 agaaaaagat aagcaacagc agcctgctgt aggacctgac tccaactttt cccaaaaggt
    35941 actgagctct tttgaaagag agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa
    36001 atggaaaaag tatacggaca caagaaatga accgccaaaa gcaaatgatt ttcctaagta
    36061 tttctatgca ggttttttta tccgaatgtt tgctttcttg gtcgatcttt tgtgtatcgg
    36121 agctattacg cgtttaacac ttggtattgc caaaaatcta ggttggctta atttttcaga
    36181 ttcttatctg agtatctacg gattcacagc cttacttatt tatctagcgt attttatttt
    36241 attgacgaaa ttcaatcatg gtcaaacgat tgggaaaatg atctttggta ttcgagtcat
    36301 cagctttgat gaagaagaac ttagctggtc aaccgtttta gttagagaag gtgcttgccg
    36361 gttcattcta aaatttcctt tgctgatgtt agggtatctt ccagcaatct ttaatcagaa
    36421 aaagcaacat ctaggcgatt ttttcactaa tacaagtgtt gttacgatca atctgatcaa
    36481 agcattcaac caggaagtca acagctgatg agcaataaaa agcagtaaca gttgaaagca
    36541 ccttctaaaa gtcagatcta aaatctagct tttagagggt gcttcagtta tttttaattg
    36601 tcatgctttt tttgttttgc tactattgaa catgagctga acatgagcaa gaggaggaca
    36661 ctgcgtgaag ccgattgatt ttccagaaaa atatgagaat ttaatgcgag tagcacaaca
    36721 agcactagca aaccagcaat atcagcaggc aaaagaatta tttcaacgag cttacgagtt
    36781 gaaagaaagc tttgaggcta atagcctgct tgttttttgt ctttacgagt tggacgagaa
    36841 aaaagaagca ttgaagcaag cactcttgca tgaaaaacag tatcttcaaa atgaggagtt
    36901 tgctgaattt tattttgact tgttaatcag tacacaagat tttttatatg ctagaaaatt
    36961 gatagcaagt acagattttt atgaagcttt cgagcagcgg gtcattgaaa aaatccaatt
    37021 tgcagaagaa ttatccggac aaatggagag gcagaagatc aaggcactcc acaataaatc
    37081 ggaggaactt ccttcgttgg aacctgcacg tcagcttgcg tctattgatg agatcgaaca
    37141 gcttccttat catgagttta tccaaacagc gagtaaactt attgtatcgc cagaggtcca
    37201 tattttagca agggcaaaat tactggagac acttaggcaa ttgaacgaat gccatcctgt
    37261 tttctatttg acgattgaag aaaagttagt caaagtcata ccaaaagatc tgccaaagcc
    37321 gcagcagcag gcaagttacc agcaattatg tgtattttca gatcgttacg gtaatgaaga
    37381 tgcgctgctt agttccgtgt taaatgaaga attcacgttg caatctgcaa ttgtttatcc
    37441 tgtctatgat acatacatcg aagacccgaa gcgctggttt caactgactg tagaggcgtg
    37501 tacaggaaaa acgatgtatg aaggtacaga agatgaaaaa caagattttt taaaaaaaag
    37561 agaaaaaatt ttgcagcaaa tgatcttctt ccattagatt ttatcagtga aaaagataaa
    37621 aatgttatga aaaacaagga ttctatccat tcaatatgtt tacaatttga aagggttagt
    37681 gtactattta aaggtatgca tgaaacgttt ttgatgtata aaatgaattt ctaaactcgg
    37741 agggaattaa atgactgcaa catttgaaaa aaaaggcacg aatgatggtg ttttaaaatt
    37801 tacgattgcg caagacgaaa tccaaaaagg attaacaact gcgtttaata aagtgaaaaa
    37861 aagcttgaac gtacctggat tccgtaaagg gaaagtttct cgtcaagtat tcaaccgtat
    37921 gtacggtgaa gaagctttat atgaagatgc attgaatgct gtattaccag aagcttatga
    37981 agctgctgtc aaagaagctg gcatcgatcc tgtttctcaa ccaaaaatcg atgttgaaag
    38041 catggaaaaa ggtcaagact gggtaatcac tgctgaagta acagtgaaac cagaagtaaa
    38101 attaggacaa tataaagatc tagttgttga aaaacaagac cgtgaagtaa cagatgctga
    38161 cgtggatgca cgtatcaaac gtgaacaaga agcacaagct gaattagtta tcaaagaaga
    38221 tgctgcagct gaaaatggcg atacagtagt aatcgacttc gaaggctttg tagatggcga
    38281 agcgtttgat ggcggaaaag gcgaaaatta ctcacttgaa ttaggttcag gctcattcat
    38341 tccaggtttt gaagatcaat tagttggtca taaatcaggt gaaagtctag atgtaaatgt
    38401 aactttccca gaagattatc aagcagaaga tcttgctgga aaagaagcag tcttcaaagt
    38461 gacaatccat gaagtaaaag caaaagaagt gcctgaatta gatgacgaat tcgcaaaaga
    38521 tgttgatgac gaagtatcaa cattagacga attgaaagaa aaatatcgca aagaattagt
    38581 agaaacaaaa gaaaaagcag cggacgatgc aaaagatgaa gcagcgatcc gtatggctgt
    38641 tgaaaacgca gaaatcgttg aattgccaca cgtgatggtt catgatgaag tacatcgttc
    38701 aatggacgaa ttcttgaaca acatgcaacg tcaaggtatc tctcctgaaa tgtactatca
    38761 attaactggt tctacagaag aagacttgca taaacaattc gaaggtgaag cagaaatgcg
    38821 cacaagaaca aatcttgtga tcgaagcaat tgcagctgct gaaaatcttc aagcaacaga
    38881 agaagaaatc aatgcagaaa tccaagaatt atcagaaaca tacaacatgc caatcgaaca
    38941 aatcaaacgc gtgttgacag aagatatgct ggaacatgat gtgacaatga aaaaagcaat
    39001 cgaattgatc acaggtacag cgacagaagc gaaagaatag aaatcaagta ataaatcatt
    39061 tctgaagaag ctacttctag aaaattgttt attcgaaata agggactgtg acacgatttt
    39121 gtcacagttc cttatttttt gttaagagta aaaaatgcaa aggtataggt cagttgatcc
    39181 tttgttaaac actttttctt tttactaaac tatgttatga tagtaaaaga ctgtaaagtc
    39241 tttgatagtt tcttgcatac tttttgcaag ttaaggggtg aagaccatgt atgacaatcc
    39301 aagtagcaat gagaccgtac gctgttcttt ttgcgggaaa acccaagaag aagtgaaaaa
    39361 gatcgtagct ggtccagggg tctacatctg taatgaatgt atcgacctat gtaaagaaat
    39421 cattgatgaa gaattttatg aagaagctgt acgcgaattc actgaagtcc ctaaaccact
    39481 agaaattctt gaagtattga ataactacgt gattggacaa gatcgcgcaa aacgctcgtt
    39541 agctgttgcc gtatacaatc actataaacg tgtcaatcag caagcacaag aaaacacaga
    39601 tgatgttgaa ctgcaaaaaa gcaatatctg cttgatcgga cctacagggt ctggtaaaac
    39661 atttttagca caaacattag caaaaacatt gaatgtacct tttgcaatcg cagatgcgac
    39721 aagtttgaca gaagctggtt atgtggggga agatgtagaa aacattttgc ttaaattgct
    39781 tcaatctgct gattacaatg tggaacgtgc agaaaaaggg atcatctata ttgatgagat
    39841 cgataaaatt gcgcgtaaaa gcgaaaatgt atctattact cgtgacgttt ccggcgaagg
    39901 tgtccaacaa gccttgctga aaattctaga aggaactgtt gcaagcgtgc cacctcaggg
    39961 aggacgtaaa catccacatc aagaatttat tcagattgat acgacgaaca tattgttcat
    40021 cgtaggtggt gcatttgatg gcatcgaaac gattgtgaag aatcgtatgg gcgaaaagac
    40081 gattggattt ggtacacaaa accaaaaact ttctgaagac gaaagtgtca tgcagcatat
    40141 cattcctgaa gatttactaa aattcggatt gatccctgag tttatcggac gattacctgt
    40201 tatggcagct ttagagaaat tgacaacaga tgatctggta cggatcttga ctgagccgaa
    40261 aaatgcgcta gtcaaacaat atcaaaaact tctttctcta gataataccg aacttgaatt
    40321 cgaacctgaa gcgttgagag ccattgcgaa aaaagcaatc gaacgcaata ccggtgctcg
    40381 aggacttcgt tccatcattg aagaaatcat gatggatgtg atgtttgata tcccttcaaa
    40441 tgaaaacatc gaaaaagtga ttatcacaaa agaagcagct gaattgtctg gtgagccaac
    40501 agtaatctat aaagattcag agaaaaaagc gggttgatga aaagaggctg ccgtaaaagt
    40561 cattttggct tttgcgacag ccgcttgctt tattcatcac cttgttaagg agagagacca
    40621 tgaaagtcca tcaagctgaa attatcataa gtgcagtggc accaaaacaa tatccagata
    40681 cacaattgcc agaaatagct ctggcaggac gatcaaatgt agggaaatct tcttttatca
    40741 acacattgat tgatcgaaag aatttagccc gtacttccgg taagccagga aagacccaga
    40801 cattgaattt ttatttgatc gagaacgcat tacattttgt cgatgttccg ggatatgggt
    40861 atgcaaaagt atccaaaact gagcgagcaa aatggggaaa gatgatcgag acatatttaa
    40921 cgactagaga ccagctacgt gcggtcgttt ctttagtaga tttgcgtcat aagccttctc
    40981 aagaagatat ccagatgtat caatttttga agtattatga tatccctgta attgttgttg
    41041 ccactaaggc agataagatc cctcgtggta aatggaacaa gcatgagagc gaaatcaaaa
    41101 aagcgttgga ttttgatcgc aatgacacgt ttattctctt ttcttccgtt acaaaacagg
    41161 gaaaagaaga agcgtgggca gcaatcgaac aagcaattga atcataaaaa gaataaaaaa
    41221 gacttaccag tcaaactctt tcataaaa
//
LOCUS       DACESX010000009        39471 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.009, whole genome shotgun sequence.
ACCESSION   DACESX010000009 DACESX010000000
VERSION     DACESX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.009"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(114..659)
                     /locus_tag="IXF88_000400"
     CDS             complement(114..659)
                     /locus_tag="IXF88_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1507157.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(671..1363)
                     /locus_tag="IXF88_000401"
     CDS             complement(671..1363)
                     /locus_tag="IXF88_000401"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1507158.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1382..1666)
                     /locus_tag="IXF88_000402"
     CDS             complement(1382..1666)
                     /locus_tag="IXF88_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507159.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1679..2242)
                     /locus_tag="IXF88_000403"
     CDS             complement(1679..2242)
                     /locus_tag="IXF88_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1507160.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2470..3132
                     /locus_tag="IXF88_000404"
     CDS             2470..3132
                     /locus_tag="IXF88_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1507161.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3256..4452
                     /locus_tag="IXF88_000405"
     CDS             3256..4452
                     /locus_tag="IXF88_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1507162.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4553..5929)
                     /locus_tag="IXF88_000406"
     CDS             complement(4553..5929)
                     /locus_tag="IXF88_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1507163.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5966..6832)
                     /locus_tag="IXF88_000407"
     CDS             complement(5966..6832)
                     /locus_tag="IXF88_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1507164.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6921..7946)
                     /locus_tag="IXF88_000408"
     CDS             complement(6921..7946)
                     /locus_tag="IXF88_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1507165.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7964..8638)
                     /locus_tag="IXF88_000409"
     CDS             complement(7964..8638)
                     /locus_tag="IXF88_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1507166.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8822..9715)
                     /locus_tag="IXF88_000410"
     CDS             complement(8822..9715)
                     /locus_tag="IXF88_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1507167.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9789..11486)
                     /locus_tag="IXF88_000411"
     CDS             complement(9789..11486)
                     /locus_tag="IXF88_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1507168.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11479..12792)
                     /locus_tag="IXF88_000412"
     CDS             complement(11479..12792)
                     /locus_tag="IXF88_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1507169.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12792..16310)
                     /locus_tag="IXF88_000413"
     CDS             complement(12792..16310)
                     /locus_tag="IXF88_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1507170.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16603..17061)
                     /locus_tag="IXF88_000414"
     CDS             complement(16603..17061)
                     /locus_tag="IXF88_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1507171.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17358..17876)
                     /locus_tag="IXF88_000415"
     CDS             complement(17358..17876)
                     /locus_tag="IXF88_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1507172.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17878..18495)
                     /locus_tag="IXF88_000416"
     CDS             complement(17878..18495)
                     /locus_tag="IXF88_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1507173.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18492..19346)
                     /locus_tag="IXF88_000417"
     CDS             complement(18492..19346)
                     /locus_tag="IXF88_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1507174.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19343..20254)
                     /locus_tag="IXF88_000418"
     CDS             complement(19343..20254)
                     /locus_tag="IXF88_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1507175.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVQLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20295..21629)
                     /locus_tag="IXF88_000419"
     CDS             complement(20295..21629)
                     /locus_tag="IXF88_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1507176.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21837..22667
                     /locus_tag="IXF88_000420"
     CDS             21837..22667
                     /locus_tag="IXF88_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1507177.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(22911..23160)
                     /locus_tag="IXF88_000421"
                     /pseudo
     CDS             complement(22911..23160)
                     /locus_tag="IXF88_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23351..23545)
                     /locus_tag="IXF88_000422"
     CDS             complement(23351..23545)
                     /locus_tag="IXF88_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507178.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24050..24235)
                     /locus_tag="IXF88_000423"
     CDS             complement(24050..24235)
                     /locus_tag="IXF88_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507179.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25085..25426)
                     /locus_tag="IXF88_000424"
     CDS             complement(25085..25426)
                     /locus_tag="IXF88_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1507180.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25426..25656)
                     /locus_tag="IXF88_000425"
     CDS             complement(25426..25656)
                     /locus_tag="IXF88_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1507181.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            25943..26281
                     /locus_tag="IXF88_000426"
     CDS             25943..26281
                     /locus_tag="IXF88_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507182.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26486..26725)
                     /locus_tag="IXF88_000427"
     CDS             complement(26486..26725)
                     /locus_tag="IXF88_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1507183.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(26701..27048)
                     /locus_tag="IXF88_000428"
     CDS             complement(26701..27048)
                     /locus_tag="IXF88_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1507184.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(27780..28361)
                     /locus_tag="IXF88_000429"
     CDS             complement(27780..28361)
                     /locus_tag="IXF88_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1507185.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28544..29170)
                     /locus_tag="IXF88_000430"
     CDS             complement(28544..29170)
                     /locus_tag="IXF88_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1507186.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29192..30523)
                     /locus_tag="IXF88_000431"
     CDS             complement(29192..30523)
                     /locus_tag="IXF88_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1507187.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30678..31079)
                     /locus_tag="IXF88_000432"
     CDS             complement(30678..31079)
                     /locus_tag="IXF88_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1507188.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31165..31371)
                     /locus_tag="IXF88_000433"
     CDS             complement(31165..31371)
                     /locus_tag="IXF88_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507189.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31466..32968)
                     /locus_tag="IXF88_000434"
     CDS             complement(31466..32968)
                     /locus_tag="IXF88_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1507190.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(32986..33441)
                     /gene="argR"
                     /locus_tag="IXF88_000435"
     CDS             complement(32986..33441)
                     /gene="argR"
                     /locus_tag="IXF88_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1507191.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33539..34156)
                     /locus_tag="IXF88_000436"
     CDS             complement(33539..34156)
                     /locus_tag="IXF88_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507192.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34377..35750
                     /locus_tag="IXF88_000437"
     CDS             34377..35750
                     /locus_tag="IXF88_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1507193.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            35825..36937
                     /locus_tag="IXF88_000438"
     CDS             35825..36937
                     /locus_tag="IXF88_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1507194.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37009..38646)
                     /locus_tag="IXF88_000439"
     CDS             complement(37009..38646)
                     /locus_tag="IXF88_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1507195.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(38814..39422)
                     /locus_tag="IXF88_000440"
     CDS             complement(38814..39422)
                     /locus_tag="IXF88_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1507196.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        11357 a         8393 c         6838 g        12883 t
ORIGIN      
        1 agaataagtt tttttaaaat aacgtataaa aaagagcata aaacaaagtc gtttgtttta
       61 tgctcttctt ttattatttt gtgcttttta ttttatcaaa tcactttgtt tttttataaa
      121 atttctgcgc ctagtgtgga ctcaaaatgt ctcaatgccc attcatgtcc aacgggatcg
      181 aaacttgcca cggcatcttt gaaaatatgg agtttgtatc ctagattata cgcatcgact
      241 gctgtatgca acacacagat atctgtacat acgccggtca aataaatatc tgtgatctgg
      301 cgttctcgca aacggatgtc tagatctgtt ccactaaaag cggaataatg ccgtttgtct
      361 atccaataaa cattttcctc ttgctcatag tcttggtata aactttttaa agagccgtac
      421 aattcccgtc cacccgttcc tatcacatta tgcggaggaa ataagcggtt ctccggatga
      481 aaggaatctt ttggatcatg cgcatcaatg gcaaaaacga caaaatcctt ttgatcaata
      541 aacttctttg tatgagcagt cagctttttt tcaattactt gtcctgctgt cccagtagtc
      601 agctttccat cctctgcaac aaaatcatat gtgtagtcaa tcgagatcaa tgcgttcatc
      661 ttagtcctcc ttatacgaga tgtttgacaa attcgatcac catttctgct gatcgtttgc
      721 ctgcgtcttc gataaattcg tcaaagcttt gtgtcgccga gtgatctgct gtgtcgctca
      781 ttgcacgtac gatcaaaaat gggatattga actgacgagc tgtttgtcca acagcggctc
      841 cttccatttc gcaagctaat gcttcaggga aattcgttaa gatttctttg attttatctg
      901 gcgagtcgac aaatgtatca cctgtaacga tcaatccttc ttttgcgttc aaattcgttg
      961 cttttgctgc tttaaccatt tcactgcgca aatatgtgct tgcttcataa tatagaggca
     1021 ttcctggtaa ttgtcctggg ttgtagccaa atcctgttgc atctgcatca aaataagcta
     1081 ctttgtctga tataacgatg tcacctactt gtaagccttc accgattccg ccagctgaac
     1141 ctgtattgat caccatgttc acgccgtatt gttggatcaa cagacttgtg gtaatagatg
     1201 ctaagacttt tcctataccg gaacgtacga cgatcacttc gtggttacct aatgaacctg
     1261 aaataaataa agcgcctgcg cgttcccatg ataatggttc gcttaggttc tctctcaaaa
     1321 tttttacttc ttcttccatt gcgccgataa taccgatttt catcttttta attccttctt
     1381 tctatataaa tgcgattgct aaaaaaacca cacataacat taggataaca ataataagag
     1441 atttcatcaa aaaagagttc catttggtcg tcttttcccg ttcgctgatc cttgttttcg
     1501 tgattggttt attctttttg ctttctttac gataaaagct ggcaatcttt ttttcttctt
     1561 gctgataagc tgtttctgct tttcgctgtt tttttaatga ctcgcttgct tgtgcttgtt
     1621 gtcgcttgcg aagttcgctt cgcgtaataa gcggtccttt ggccataagc cgatccctct
     1681 actttccttt ggtcaatagt tcccagtatt gaatcgcata aatcgtcttt gcatcacaaa
     1741 tcaactgatc tttcatcgct tgttgcgcct cttcaagagt tagatgatac aactccagta
     1801 cttcgtcttc atcttgtgct aaaggattct cgaccttctc taccccttgt gcatgataaa
     1861 tatgaaggac ttcatttgca aaacctggtg aaagatacat cgaagtaaga tggctcagcg
     1921 acttcgcacg atagccagtt tcttcttcta attctctagc tgctgtcatt tcgggatttt
     1981 gtccttctcc tggatcgatt tttccagctg gaatctccaa aatcactttt tctaaaggtt
     2041 ttcgaaattg tttgacaaga agcagacgat cttgttcgtc aaaagcaatg atcccgacac
     2101 cgccaggatg aaataccagt tctctcttcg aagttcctcc atctggcaaa cgaacttcgt
     2161 ctacagcaac atcaataatt tttccgctat agatttcttt gcgactgatt gttttttctt
     2221 caaattgctc attgttcagc atatcttctc ctgctttctt tgtaatcata actgcttttt
     2281 tttcattttt caatttttct caaaaactcc ttattagttt accatagata aaccttagat
     2341 tttctctaaa tatagaaaat ccttctttag tccgatactc tttatagtag tccgtatgct
     2401 aaaataagta tgtaatcgaa taacgaaaag aattcaatac tcaacgataa atgacatgga
     2461 gggtttatta tgataaaacg aaattggccg tggcttctag cattggtctt gctatttctc
     2521 tttgtccgaa acaatagttt ttcttttgtt ctaggacttt tattgatagg tgtagtttta
     2581 gttttgtggg gactctttgg ttcgcgcagg aaaaagggaa aacaagaaga aattcctgca
     2641 ctgactgacg aacttgaaac tcactatgaa aaaagcggta tgacagcgaa tgaaatttca
     2701 ttttttcgac aaacaatgaa tcaaacaaaa aatgagatca atcaattgca aatgaatatg
     2761 cagcagacag ccaaacttaa agcaatcgat ctccgccatg atccagtcaa agcagcgaaa
     2821 gctttattca aagaattggt gaaagaacca aaccgactgc atgaagcaag tcagtttctt
     2881 tatacgcatc tgccgaatct agttgatttg acaaataagt ataatgagat caatgaccat
     2941 gagatcaaaa acaaacagac ctatgaaaaa ctagaagaaa gcgcgcagat catcgaccag
     3001 ctgtcagctt taatcgcaca agattatcaa aaattcgttg ctgaggatct agatgatatc
     3061 gacgtagaga tatctgttgc gaaacagagc ttaaagcgtg ataataaata tgaaccagaa
     3121 caaaaggatt aactcttttg ttcataaaag aagactcgga acattcagat gatgtctggc
     3181 tgtttcgagt actttttcta aagtagagac attcatgtaa gaatcgagaa cagattcaac
     3241 aaggaggaat tttttatgga tcataaaccg actgaaacaa aagatgttac accagttaac
     3301 gatacacttg aagatttatt gaacaacccg ttttcaactc caacagatac gctgaccact
     3361 tcacaacaag cggagattga tcagctccaa gagcaacaga cagctgctcg tttaattgac
     3421 aaactaccgg cggaacgcca agaacaagct aaacagctag cagcaaaaat cgatgctaat
     3481 gatgcacagt ctgtcatcag ctacggatcg gctgcccaag caaaattaag tgaattctca
     3541 caatcgatgt tgaatcatgt gcaagcacaa gatattggtc ctgtaggaga ttccttaact
     3601 gaactgatgt atcgtctcca agaagccaat cctgacgaac tgcgtgccgg agaagggaac
     3661 atcttccagc gtgtcttcgg gaaggtcaaa caatcgatct atgaagtgac agcaaaatat
     3721 caaaagattg gtgcacaaat tgacaagatt tcagtgaaat tagataaaga aaaagacggt
     3781 ctgctaaaag ataatctgat gttagaacag ctttaccaga aaaacaaaga ctactttgat
     3841 gcactgaata tttatatcgc tgctggagaa ttgaaaatgg aagaacttca aacaacgatc
     3901 attccagaag cgatgaaaag agcagaagaa actggcgatc agatggatgt tcagattgcc
     3961 aatgattaca cgcaattcct agatcgtctg gataaacgta cgcatgatct tcgcttagct
     4021 agacagatca cgatccagca agcaccacaa atccgtttga tccaaaacac aaatcaggcc
     4081 ttggctgaaa agatccaagc atctatcgct acagccattc cgctgtggaa aaaccaagtc
     4141 gtgatcgcat tgaccttgct tcgtcaaaaa gatgcagtaa ctgctcaacg ccaagtctct
     4201 gaaacaacaa acgacttatt gaagaaaaat tcagaaatgc tgaaaatctc agccatcgaa
     4261 acagcgaaag aaaatgaacg tggaatcgtg gatatcgaga cccttcaaac cactcaaaat
     4321 gatttgatcg aaacaatcca agagacattg cgtatccaaa aagaaggaaa agaaaaacgt
     4381 cgtcatgcag aagtagagct tggacacatg gaagaagatc tgaaacaaaa actgttggat
     4441 ttaacccaat agatcacctt tcgcttgtta ctaaaacaaa cgcccaacaa cagtttatta
     4501 caactgttga tgggcgtttt tcttatcttt cttctaactt ctccattgga gtctatttct
     4561 cattcatctt gctgtgtcca tagccatagc caaaataaat gatcagtcca ataaccatta
     4621 cgataccgaa cacgatccat gtcacagttt gcagacgaag catcaataca atacaagtca
     4681 agatggagac gattggcaat agcggaacga atggcacttt gaattctcca gcttttggtt
     4741 tgccaaacat ttttctcaac ttcagtaagc ctagactaac aagcatcaaa taggcgagcg
     4801 ccatgatatt cgttagttcc gctaatacat tcaatgggaa aaatccggca aagaatacag
     4861 ctgccacacc agcggcataa gtcgcttttt tcggtgtacg atgcttttga tcgatttctg
     4921 tcataaactt aggaagtaag ccatccttgc tgacagcaaa cagtaaacga gcaagtgaat
     4981 acatcatcga gatcgttacc gtcagtagcg ttaaaattgc ccctacagcg atcaccgcgc
     5041 ccactgttcc atgtcctaca tacctcattg caaaagccac tgggtcattg actcctagtt
     5101 cagtatacgg aacgattcct gtcaagatca gcgtcacgat gatgtaaagg acagtagcaa
     5161 tcagtatcga accaatgatt cctcttggca catctttttg cgggtttttg acttcctcgg
     5221 ctgccattgc aacggcatca aatcccaaga aagcaaaaaa cacaagagct gcaccatcta
     5281 gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt atcaggttta acgaaaaata
     5341 ttcctacaat gataaacaac gcgatgatac caaatttgac atataccatg gcattattca
     5401 aacgtaatgc tttcttggct tcaagcgaca cgatgtaagt gacgaatatc actacgagca
     5461 ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta cccgccagtt aatgcttgtg
     5521 gaagttcggc ccctaaactt ttcaaaaaac cttgcatata gccagaccat ccggaagcaa
     5581 cagaggaaac cgctagtaaa aattccccga tcaacaacca ccctgtcagc caggcagcaa
     5641 attcaccaaa tacaacatac agataggcat atggcccacc aatcactggc actcgcgaag
     5701 caaattctgc gaaacttagt ccagacaaaa tgacaacgat tgcagctaag acaaatgaaa
     5761 gtgacagtgc tggaccagca ttttgattgg ccgcaacccc tgtcaccaca aatattcccg
     5821 taccgatgat cgcaccaatt ccaagcatga tcaaatcaga cgtttttagt tcttttttca
     5881 tctcactcgt atgattgaga ttgatctctt tcttacgaaa caaatccatt gaagttcctc
     5941 cccattttag gttggaaaac agcgattaaa ctgttttcat ataccctaaa aggataccac
     6001 ctaaaatgac taaaaggcaa ccaacaataa cataaatgag ctcttttttc gtttttcttt
     6061 cgccaaggat gaaaatccca cctaaagtag agataatgat ccccatttgt gaaagtgaga
     6121 agccaacagc taaaccaagt gatttgactg ttagaagcat gcagatgttt cctaagcccc
     6181 ataataatcc acagatcatg tttttccaca caaactgatc gactttaact ttacggaaag
     6241 caaagaagct tgcgcctaag atcattccga tactttgagg taagataatt gacatcgcat
     6301 ccaaattcgc ccagttgacg atgatggtat atacaccata accaatcgta gagaagatca
     6361 atgctctaaa acccttacca aaatcaagca ttttattatc tgtatctatt tttccttcgt
     6421 catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag agctaaagca ataataccgt
     6481 atacataatc tttcgtttgt gtccattcat ggaagaaaac ggctcccgca atcgtattca
     6541 aaattaattg aaaaccagta gacaatggta gccctacaga aacacccatg tacttcattc
     6601 catggaactg tccattttgt ccaacactcc aagctaagcc ggataaaaat ccgatcaaga
     6661 aaatccaagg ggtgattaca ggactaacaa cgaaaaagac aactaaagaa aacagaaatg
     6721 caccgatcgt cattcctagt gtttgttgat tggcatcgcc accaatttta ccacttacta
     6781 aaccaatact accccatgcc aacataggga ctaatgctat taaaatttcc ataaccaatc
     6841 tccttttatt tcacatgata agaaacttat cagcgaaaac gttaacaaca caagcacttt
     6901 ttttcgtttc taacgatttt ctaacgtttt tcattataaa aatttaagat tgggaaaaat
     6961 aaagtcattg ttgtcccttt tccttcagtt gattcagccg tcaaatggat acctaatttt
     7021 tcggccagat tttttgctaa ataaagtcct aatcctgttg aatgtgtttt gctaagtcga
     7081 ccatttctcc cagtaaatcc cttatcaaag atccgtttca agtcttccgc tgggataccg
     7141 atccctgaat ctttaactga aaggctgact ccttgcgctt ccttttctat gctcacaatg
     7201 atgtttccgt gatccggcgt atatttgatg gcattactaa gcaattggtt gaagataaaa
     7261 gcgatccatt tagcatcggt caaaacagta tgatcctcac caacgatctc gtagcgaata
     7321 tttttttgga taaaataatt tgcctgactg cgtatcacag gctgaatgat tccttttagt
     7381 ccatattctt ggatgaggta gtcccgcgag aaactgtcga gtcttgcata atacaaaact
     7441 tgttctacgt attcatcgat ttttgataat tcattttcta gtaatgtata cttttgatcg
     7501 tcaatatcga actcgatcga acgaactagc aatttagaag cggaaagtgg cactttgatt
     7561 tcatggaccc atgagtcaat ataatctttt tggtcttgtt gattactgat tgctgattgc
     7621 attacttctt ggtgttcacg gatcagctga ttgatatatt cttctaccag tttttcttct
     7681 tctgtcctcc caccactcaa ataattttgc aaaggggaag ttttttgtat gtcggctaat
     7741 tttccccacc atcttctctt gctcaagtaa ccacccagca gataaaaaat cagaaagacg
     7801 ccctcaatca aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt tggcgccagc
     7861 cacatcacaa agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa tgtccactga
     7921 tctttcaaat atttaaaaaa attcatctta accgctcatc ctttcaggga acgatatatc
     7981 cctgtcctac ttttgtttcg atatatcctt ggatacctgc ctgttcgatt tttcggcgta
     8041 accgattgat gttgactgtc aatgtattgt catctacaaa tcgttcgtca tcccacagtg
     8101 ctcgcagcaa tttttcccga cttaagatct ttccattgtg cttcatcaaa ataaataata
     8161 gacgatattc atttttgctc aaatcaatga tctctccgtt gatttccata cttccgttgt
     8221 caacattcaa cgtaatgcca ttgtggctca tcatttcact agataattct gtgtagttgt
     8281 aagaacggcg aagtaatgca ttgatttttg caatcagtac atcgatttgg aaatgttttg
     8341 tcacaaaatc atctgccccc atgttcatag ccatgatcat atccatattc gtattcctgc
     8401 tggagataaa aatgatcggt actttcgata cttcacgtat tttttgacac caataatagc
     8461 catccaaaac gggaagatta atatctagta atacaagctg tggttcttct ctttcaaagt
     8521 ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc aaattgccat ttctcaagtt
     8581 cctccatgat caattgtcga atgatctctt cgtcttcgac taccatgatt tttgccataa
     8641 aaaatccctc catctttctt cttgctacta caatatcaaa gaagcccttg tgaaacaagt
     8701 tggcttacct ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa tatgcgattt
     8761 aatcgcgttt gtctcttcca agcagcttaa atttattatg ttattcttaa taattggtct
     8821 attatctgtt atttttcttt cgttgttttt gtcttttaaa ttttcgataa caccaataac
     8881 cgaaaagcgt tagcactagc aatataccta gaaggagtat aagtaatcga taaagatggt
     8941 atttttttgt ctgttctatc tttgaagaga cttcttctgt aaagggaact cgtcttcctg
     9001 tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca agtgataagt gtgactagat
     9061 cctgcttttc tctaattgct aatgtatttg tttttgttgg aaggacaaca attttctcaa
     9121 cgacttcata agcgagcatc cgaccataca cttcaagata gaatcggtca tcttgctcca
     9181 tttcttcaag atccgtaaag agttttttct cagcaatacc gctatgggcg gtaatcacgg
     9241 aatgtgtact ctctcctcca gttggaaaag aggtgccttg caggactgtc gctcctctat
     9301 ctaacaaaag atcattcgtt tcatcaaata aaggcaagct aacattgatt ttcggaatat
     9361 agatggctcc gatcatatgt tgttcgtaat attcttttcc tggattttgg acatttctaa
     9421 ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg gattactgat gtatgagcaa
     9481 gcgtctgatt tttcttttca tgcactgcta attgttgctc atgtttttct tctttatcgc
     9541 gttcaagctg cttttgataa ttctcgatcc ttctttgatc caaataattg ctgagagcat
     9601 ctgctaaaaa tggatatgta aaaactgcag ctccggcaag aaacatcact gccataaata
     9661 attgtaataa aagactgatt tgggtttgag gtctattacc tattttcttt ttcattttct
     9721 tcaccttctt atttaaaaaa actgcagcaa cctttataaa accgattgct gcagcttatt
     9781 tttgttgctt atacttcaga cttcatttta gatttacggt accagctgta cgctccaatc
     9841 atcaaactta ttccgattag taagaatgcc aagagcccgt tcccaccagt agcaggaagt
     9901 gacccgcgtt tcatattcgg tactctttct ggccctggat tttctactcc tacgatctga
     9961 atttcttcat tatagcttcc ataagagatg ataaattcag taaaggctgc ctcacctgtt
    10021 ggaagtacat aaccttctgg cgccatggtc tctttcattt gataggttcc ataatctaac
    10081 ccttgaacct caaagtatcc atttccatct gcgacaaatt ctgttgcttg tccgcttgtt
    10141 gcccactcaa ttctatctgc tcctgattca taaacagcta aaggcgactc ccctttataa
    10201 aatattgcgt attcagaacg gtcttcattc cacaattcga atcttgcatc tttaagcaat
    10261 tcactgcttg atgcatcata tttttcaaac tttcttccac ctgttgtgac tttctctggc
    10321 ggctctactc cttcttcata atcaaattca tcatttcgtc cgatttcaat aaccgctgtg
    10381 ttgttatgga aatcatccgc ttctagttct gtggaaacct tcaaattata cgtgatcgtt
    10441 aaatctcctc cacgtgcatc tgctaatcta cctaaagtat ctactttggc tactccattt
    10501 tcttctgtca aaatcgtcac gacaaacccg ttgcttacta cttctactat ataatcttgt
    10561 ccttttgtta aaatcgtctc gccggctcgc acttctattt cctgatcata ataagttaga
    10621 ccaactgtag gcgtatctgt gattttgtaa tttttgataa cagcgcttcc gttttcaagg
    10681 acagaatcga tgaaataagg aataggtgct gtgatcgtgt aagaagccgt ttttcctctt
    10741 tctagatcaa tataagaaat cgtttcttgt tctccagctt cattaataat cgttacttgt
    10801 ctaaggtcgc tttcctctac attcatctgt ttatctgctt ctctgatttc gtttttcgga
    10861 tacaaatgca catctgtatt gtatgtgtct ttccatgtag aaccatccca cacgccatcc
    10921 accatatcag gcatcatcac tggcatcata acaaccattg gagacgcgat tctggaaata
    10981 tgagctggcg aataagtttc taagaaaaga tagactttat ctctagaaga aggtgcttct
    11041 tccgtcactt ggacggtaga gaataccgct tctccagctt cattcgttgt ttgtttgtca
    11101 atacttgtca gtcgatattt taaaaaccaa ctttctgtgt gccgtttttg aatccattca
    11161 atcgcttcgg cagaattaag accgtcttct cgttccggtg ttaaaggatt atctcgaatc
    11221 aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat cgactcctgg aatattttct
    11281 tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt cttccggaac agatgagaaa
    11341 ccttttttgt gtaatgtaat cgtcacttca cttggttttt gtgcgacttc ttcggctttg
    11401 actagttttc cgttcccgaa aatccctatt cccgcagtaa cgagtaacag tacactcatt
    11461 cccgtgacaa tcattttttt attcattttt ttctccccgt tcttttttta ttaataaaac
    11521 agcacctaac aataaaagaa ttcctagtaa agaaatcaac gttcttgctt cattcgtttg
    11581 cgggaatacg gaccattttc ctgattgacc tggattgctt ggaccactcg gctctttcgg
    11641 tttgtccgtt gtttctttta cggtctcgtt ataaacacgt ggtgttgcgg attcataggc
    11701 agagactgga actttcccgg attctaattc agccatctcc tgtccgttaa ctactacata
    11761 ttgtggttgg ccttttgcat ccacccaaaa ttcagggatg atgacctcga ttctcttact
    11821 tgcttcatcc atctcgtaac cttccacacc ttttaattct tcaaaataat aggtgccaga
    11881 aggcaagaaa cgttgtcctg tcgtaacaag gccctcttta tccgaaatga atttgcttac
    11941 atttttgtca ttcaaaggat cattgtctgc tggtttgatc cattgatttt tcaagtcatt
    12001 agcaggagcc atatctaaat aatatttccc cccttctatc atctgatata gagcaaaaac
    12061 agcaccttct aatggttttc ctttttcagt tgttcctctt tgacgtccat acttgaagaa
    12121 gtatggatct cttagataac cgacattttt aggatagata tgaatctctt gcagttcctc
    12181 ggttgattct gtcgggtgat aaattggcaa aatagcagca attgggatag ctgtctgatc
    12241 gatatcaaca tccaatccaa cttctggatc aatcgattct tcgaaaatca aataaactgc
    12301 atcccgatct tggtttttct taggtgcttt gatacgtgca atcccgtctc ctaatccgcc
    12361 aaccgtatct ttcccagttg ttaccgaagt ccctatttgg acaagccttt cttctgtgat
    12421 tagctgacgg atattgtttc tgttcgtttc agcgatttgc tggacgaatt ctttaggagt
    12481 catgttttca gcagcttttt tttcatgata ataatcggtc atgtcgtaaa tggcaaatgt
    12541 cgctccattt agtggcaccg tctgtgaaat gatatctgct gtttcccctg cttcctcaga
    12601 gatagccaag ccatcatttt catggtattc aaattgatcg ttataatcga tatctcgaat
    12661 catccgttta tgcaacacga attccacgac atcttcccct gattcttctg ccgacatcgc
    12721 atgtggaaga aggactgctg caagcggtag taaaacaagt aattgaagca tcaaacacaa
    12781 cagctttttc attaccttgc tccctccttt ttagattgtc tgacaaacaa accataggct
    12841 gctcctgcaa gcgccatgaa gaatgcagca agcaggatat agaataatcg tccggatcct
    12901 cctgtagcag gcaacggatt ttcaggatcg ttcccgacac taagctgtaa tctgattcga
    12961 tttgtctcca aaagttcata ttctaactct tgctgtagat caggcgtctc tgcttcttcg
    13021 tttggataat acgaaatctc aacttttagt gtacccgctt cttttcctgg aactacccga
    13081 tcattttcta tgtcaggttc ttctcgataa ctaatttcta aaataaagta acctggaagt
    13141 tttctatagc tgtcaggtga cttcacttcc ttgatccgat agaccccttc caacaagtct
    13201 gaagcattga atagggaaga atcgtttgga tctggtgcca agcctccctc gatgatttca
    13261 tactctcctg cactattttg tttctcaatt gaaaattctg cgccttcaag acgtcggcta
    13321 ttcatactgt caattttcaa aagttccaaa tcgattggcg ctagttcatc ttctaaggtt
    13381 acttgtagta caccatcgat gatttcatag ccttccggca agggagcagt ttcgtcttcg
    13441 atgaccgtat cttcaatgat aaacttacct tcttcattca gtgcaaactg gaacacttga
    13501 tcagatagcc gatacccatt tggtgcgaca gattccgaga cctcatattc accaatagac
    13561 agatgcagag gttggtcgta atttaaatcg acatactttt ctactcctac ggcacgagaa
    13621 tagaagtatc cgatcccagg caaacgtttc gattcttctg gttctaacgg attaccagtg
    13681 tatgctcgat acagcgggaa ggaagaatcc cccactttcc tgatatcgaa acctgcacca
    13741 tctaatgtat ggccttcatg gtctaatttt ctaactcgga tctcccgata atcaaacgta
    13801 ttgtaagcag tgaatgctac ccataaacga tcctcttcat ctgttgtata cgtgagatat
    13861 tgccaatttt cttcacctgt ttgcttgagt tggatagtcc ctatagcatt atcgtaaagt
    13921 cgcaagatcc atgtgtcatt ttcatcaggt tctttgaaac cttctgggtt ctggctctca
    13981 atgattttgt attctgcata gttggtagtt tcttcactat ccttataaac aaatgaatct
    14041 ggctctccga ttggatgaat aactttctca ccatccacga ttacatattt cccgtcttca
    14101 tctactgaat agaacgtgag tctccctttc tcttcctcta cttcgttcac accagaaagc
    14161 cctttcgcta ctggatatcc gatttccagt ccgttaccat ctgtttcata aagttcaaac
    14221 tctgcgccat tgattggcat attactgcct tcaattagtt tattgatatt catgtggatt
    14281 ggcttgaatt catttcgcat ttccagtttg acatacaact catcatactc tgtgccttct
    14341 atgttaggct cttgtacaac agaaagcttg tgatattctc cttcataata ggaattcgga
    14401 tctttacctt ccggtccgcc gactctcgaa ccagatgtat attcatctac aagctcgtag
    14461 tgacctaaca tctcatcttc gttggctgca tattttttga tttggaattg atacgtctca
    14521 tctactaact gataacgtgg tggtccagcg atctctctga tccaaaatat gtcgctgact
    14581 gctccagttt catctcctaa aatcggcatc ttcgtttcaa agatcgctat tccgtccgtg
    14641 gtagttttac ttgtttcgat tggttcggct tccccactct ctggattttg gtcatataat
    14701 gcaaattcga ctccagccaa cggctcgcca ttttgaccct tcttgctgac ttggaaatta
    14761 cggcctttat gttcgtaacg aatggaagaa gatgggaaat acatccgtcc agtagtgata
    14821 tcattctcat aatcctccag gttctgaata taggccatat tgttcactgt gcaaaaattt
    14881 ccgtcttgag ctcccgcatc aagttttact tggtacttga tcctgaccat ttccccttca
    14941 cctaaagaaa tcggagaaaa tttataagct tggttttccc gtccgacata cgttgtcgga
    15001 gtcgccacta cttctggtgg ggcttgtcca tgacgttctt catcccatat ttctggctct
    15061 tggtacttca tgccgttttc tcctggatct agatggtaaa actgaatatc tgccgatctg
    15121 ccagctccaa atggtgagac attaaaacca gtttcatcat aaagtgtggc ttgctggatc
    15181 gttctcttga aggtagaaga gatatcctcc aaggctgtaa caatgtcatt cttgttgttc
    15241 gcattgtaat atcgcggtgt tacgccatct tcttctaaat cagcaatatt tttcaataca
    15301 tttcgtccaa tagcatctag tcgttgtctc ccgctaacgt ttccatcgat tcctaaacca
    15361 atggagaaga tacttggcgg gttctcttga tacttgtttt tcagccaatg atgataagca
    15421 actgtgttga tcgcagatga cttttcttgc gaagtaggac tgccattcgt attgttatta
    15481 gaagaaccat caccaaccca gtgagtcgct ttattatctc cttcttttac ctctccataa
    15541 cgatagtgta catttacttc atcttctgga cggttaaagt cagatgcata ggtaactgga
    15601 tgagtaaacc ctgcattaga agtattccca gaaaactcct caaaatttct gaacaaaacg
    15661 tcattatctt cagcaatctt atcttccatc acagtccagt tgctccaagc tccattagtg
    15721 ctcgtacgat aacttgactg aatcggtgca taactgaagg tcggtgtccc atctcccaca
    15781 acgattagga ttttttctgg atttctattt tctccgccat tgtctttata caatgtttca
    15841 tagccatttt taagccctag tgtaagcgga gttcctccga ttggtgtacg atttaatgct
    15901 gtattctcac ttcccgtcaa acgattgata tcatttgtca atggaaagat gtccgaactg
    15961 atttgctcat ggttattgat agctgtacta ttatggtaaa aattcaccat accgattcga
    16021 atatccagac tcgggtcaga gagcagatct ttactcattt cgtttactgc ctcaatcaac
    16081 gcagcatttt tagtttgact attggtccca gcggttaatt cactcatact cgctgactta
    16141 tctagaacaa aaacgatatc gattggactg atttcctttt tctcgccccc gatgacatct
    16201 agatagacat tgtaaagggt tgttgcatct tcaggatgta gatcagatgc tgtgctgctt
    16261 ggcagaacag gttctaccca ttttttgatg tacgccccgt tataatccaa gtaactgttt
    16321 tcaaaattcc gttcatttcc ccaaagctga acggtttgag ctggaacgtt tctatcttct
    16381 tcgcctgcag ttgatccatt attagtagca tagtcgtaat ttcgccagtt cgctcccccg
    16441 tttgtataat cactatcatc gatcaagttc tctccgtcat atggcgagcc tttgtctcct
    16501 tgatagcgtt cattgctttg tgtccaattt cctaacactt ttgtatagtc attcgttgta
    16561 aacttcatct attctttcat caaccaccac ttgaagaggt agttaccctt cgttcacatc
    16621 ggggatcttt tctgtttcaa aaatcaatgt gccggtttct gcttcttttg aataaacttt
    16681 tccgacatac cattcttgac catcgatcgt ctcgactttt aggtcttctc catcttcatc
    16741 ataggaggat agacctgttc cacgaacgtt ttttacggta ccaatcccgg cggctgccgc
    16801 atcgattgct aaacgtattc taccttcatt ggcactttcc tttttttcaa aagccagatg
    16861 ccattcaagg gtttcttctt tttcaatata cttcccttca atttttgcat agtcctcatc
    16921 taagaatacc tcatcctcct ttgtttctgt tgactcagca gctacatcca gggtcgtcat
    16981 atgatagact gactggaaat atggtccaaa tagcaaactg acagttacta actgcaaaag
    17041 cttcaatttt aaccctttca catccttata ctcctttcaa aaatatttgt cttctttcac
    17101 aacaatcaac aaaacaatgc ccatctaatt ataatttaat ttaattttaa ttttaaatca
    17161 aatgatatcc tttttagtta aaaacaccaa ttatacaaca tttattatta aaattaaatt
    17221 ataacatttt ataacgttct catttaacaa aacttaagtt attaagacaa atgaataaac
    17281 aactacgctt tcctcgaaac aaaaaaataa agcacaaatg cgcatttacg cactcatgct
    17341 ttattcgtac aattaattca gataacggca gcgcatagga tgccaataac acttcctaaa
    17401 atagctcctc ctaaaatgtc agtaggataa tgtaaaagta aaaaaatcct cgaataagcg
    17461 atcaaacaag caaataaaaa ggctaatatt cctgcaactg gacttaattc gaacaacgca
    17521 gctactgata aaaaggctag cgctgtatgt cctgaaggaa aagaagaagt cttcggttta
    17581 cttgtgtaaa ggacaagccg acgatttttt tcataaggtc ttttccgtct tgtcgttttt
    17641 ttgataaaaa gcaaaccaac aaaaaaatag atcaaactgc cgataagaat ggctagacca
    17701 tattctctag tatcaggtat caagactaaa acagcagcaa ggagcaacca aataaaacca
    17761 ccattagaga tattactgat aaatttcaaa aaattgattt ttcgactatc cgttttccgt
    17821 tgcttggcga acttgttgat aatactatca tcgattttag caatcaaacg caacatgtta
    17881 ttcctccttt ttaagaacct tttcttgcgt acgatggata aaaagcagga cttgaaaata
    17941 aaacaagaaa ctgaaaccta ataaagcaaa atagattggt cctgcaaacc agaatgcgac
    18001 aagaaggatc aacgtgcctg ccaaatagaa aagcgcgctt ttgaaatcat taaaaaacaa
    18061 gaaaaatgca gtaatccaag aagcttcttt ttgttccagt tgaagcaaga ttttgtaact
    18121 aaagagaagc gcgcttaata gaagcaaaaa caacgtagta tacatcaatg tatagccaat
    18181 tcccccaccc caatagcgga aaatctgata atcgatcaat agaagaagaa aataaacact
    18241 tgctaaacta tcttgctttc cataaagaga gaagttctca cgaaactttt tccagaatag
    18301 tgaaccgagc tttttctctt catttttttt gtaatgataa atcatataca gaactgttcg
    18361 acttgcagtt cctaatgtga aaacaggcaa gacgcacagg aaccatagta gctgtaaatg
    18421 gataagacgt ggaatccatt caatgatttc taagaaataa tcattggctt ttttaccaat
    18481 cgatcgattc atcatccttt tgaacctcct gtcaaatcca tcccttcaat gaaataacgt
    18541 tgtgcaaaaa agaacagcaa aatcgtcggg atcaagacaa gtgttgctcc tgccatcaga
    18601 taattccatt gtgtcgttgc ttggttctga aaaacttgca agccaatctg taacgtgtat
    18661 ttttcttgat cttggatata taacagagga cctaagaaat cattccaagc tgcgttgaag
    18721 gaattgatcg caatggtaat caaggcaggt ttcgtcaatg gaagcatcaa atgactccaa
    18781 atatacaaat gattcgctcc gtcgatttca gcagcttcga tcaattcatt attgatcgat
    18841 aagtaaaatt gacgcatcag aaatatattg aacgcattgc cgaagaacga tggaacgatc
    18901 aggggcaaat aagtacctac ccagccgatc ttggagaata atacatattg agggatcatc
    18961 gttacaaacc ccgggatcat catcgtagaa agaaccaaag caaacatcag ctttttccca
    19021 ggaaaatcaa gcttcgcaaa tccataagca ataaaagaat ttgaaaggac attaccgatc
    19081 actactagca cagtgatgaa taatgtattt cttgcatagc gccaaaaagg aaaggcggca
    19141 atcgtatcca gatagttttc ccatacgatt ttttctggga aaaaggtcgg aggataacga
    19201 acgatttctt ccattggttt caatgaagta aacaccatcc aaagcaatgg aatgatcaaa
    19261 atgattccta ttactgtcat gaggacataa cttagtattt tttttgtttg ccgattcaca
    19321 ctggtctttt tgaaataatc catcatttgc cgtcacctcc agaataatag acccacttag
    19381 gtgcaagctt caaattgaca aacgtcagaa ccaacacgat cagaaacagg atcattgaca
    19441 tagccatcgc ataacccata tcaaaagcta cgaacgcctt gttccacata tgcagattat
    19501 aaaaaagcaa agaatttgca ggtccgcctg ttccattttt cgtcatgaca taagcttctt
    19561 gaaaaatttg gaaagaaccg atcgtagagg tgatcacatc gtaaaaaata ataggagtga
    19621 ttaatggcaa agtaatactt ttgaactttc cccaaacgcc tgctccatcg atttctgctg
    19681 attcatataa agatctagga acattttgta atgtagctaa gtaaagtagc attgctcctc
    19741 cagaactcca tattttcatc aaaatcaacg caggtttcgt ccattgtgga tcaaacagcc
    19801 aagctggtcc agggatatgg aaccagccca gaaatgtgtt gatcaatcct gcagatggag
    19861 aaagcagctg catccataag atgtagactg ctactcctga taagactgcc ggtaaataga
    19921 aagctgtacg gaatacactg attcctttga ttttttgatt gagtactgtg gctaaaaaca
    19981 cactgaaaat cgtcgtcagc tgtacattga agatgacgaa ataaacggtg ttgtataacg
    20041 atttccaaaa taaatcatcg tgaacgaaca ttcttttgaa atttgctaga ccaatgaaat
    20101 tcatcttgga tgtcaaatta taatcggtga aactaccaat aactgaaaaa agcataggcc
    20161 ctagcataaa taccaagaac ccgataataa aaggcgagat aaataaatag gcccatcttg
    20221 cttctttttt ctttcggata gataaacgct tcaaattttc ttgcctcctc tgtacctaga
    20281 cagtcaaaaa tggattattg attttgcttg actaagtttt ctactgcttt ttgtgcatct
    20341 gccaatcctt cttttggtga ttttgttcct aaaagaatct ggtcaatctg ttcattcact
    20401 aatgtcgtgt aatccggagc atctaaagga actggtgtga tcaccgtatt tttaaagttt
    20461 tcgtccgcca tttgatagat catttttcct ttatccgtta ggttgtcatt attcactaaa
    20521 ttctcattgg ctgtcttgct cgccatgata tcaaaacttt tttcgccgaa tttttcttgt
    20581 acttctggag aagaaagata tttgatgaat tcataagagg cctcaggatc ttttgctccg
    20641 tatggtactt ccaagacgaa tccgccaccc cacgaccaat ggcctgaacc ttcttctttt
    20701 tctgggagct gcatcacgcc aaaatcaaaa tcatccggtg cattttccat caaactagag
    20761 taataattga tattttgggc acgcatggct actaatccgg aaataaatgg atcagataca
    20821 ccagacccaa attcggcttc taatttattg atcgtatttt tcccataata atcttgccag
    20881 tcttggatcc actccaaggc ttccacttta tttttagtat caatcttgac tttttcatct
    20941 ttgtcaaacc aactagtccc gttatctgcg tttagcgccc atacatcagc ccctaaattc
    21001 cataatggat aaaaaccgat gcgtgtaaat tcttttcctt cttttttatc tagtttacgt
    21061 gctgtttctt ctaattcatc ccaagttgtc ggtagatctt gttcactgat tccagcttct
    21121 ttaaataact ttttattata aaaaatgact tgtgtatctg tattgaatgg gatcgcataa
    21181 ggatcacctt catattctac tgtatcccaa agctgcggat aaaaatcctt agaagtgtct
    21241 ttttctacat atttggagag attagttgct tgctgtgctt ctgctctttg ggcaacggaa
    21301 tttatgtcct gtacaataac atcgggtgca tttccagctg ttattgcaga taaagattta
    21361 gtccagatgt caccccatgg ctgatactta taatttacat ggatctcgtt ttgtgattga
    21421 ttgaaatcct caatgatttc ttcgatagct tcttttcgtg caccggatcc ccaaaaggac
    21481 cagaaatcaa tattttttct tccatcactt gttttttcag aagaatttgc tgcacttgaa
    21541 ctggcatcgc ttgcattact tgatccgcat cctcctaata aaaacaatgc tgaacttgcc
    21601 agcaaccctg taaacaataa ttttttcata ttttacctcc aaattttaat gtaaccgatg
    21661 ccggttttaa tataacgata tcattttgtt ctgaaaataa caatttataa ggtatgattc
    21721 taaaaaaatt attaatgata ccaatacttt atataattcg cgagcgcttt ctttgaattt
    21781 tttaatcttt cgtagcagaa tgagaatgta ttaaaaatag attaggagga attttgatgt
    21841 ttagacacca gaaagaattg cagtttgaag caaaaccaga tcgtccgaat ccattaatcg
    21901 cgaaatatct gcaggagctg atcggcggcc aatatggcga gatgagtgta gctatgcagt
    21961 acctgttcca aggctggagc tgccgaggag aagaaaaata taaagatatg ctgatggaca
    22021 ttgcaacaga agaaatcggc cacgtggaaa tgttggcgac aatggttgct cgtttattag
    22081 agggtgcgcc tgttgaagat caagaagcag cgatgcaaaa agatccaaca atcggtgcca
    22141 ttatctctgg aatgaatcca cagcacgcga tcgtagctgg cttaggacct cgtcctgctg
    22201 acagcgtggg caatccatgg agcggtgcct atatcattgc gagtggcaac ttgttagcag
    22261 actttagagc taatttaaat gccgaatcac aaggaagact tcaagtagca cgtatttacg
    22321 aaatgatcga tgatcgtggc gtaaaagata tgctgtctgt tctccttgca agagatagct
    22381 atcatcaaaa tctatgggaa tgtgcagtga aagaattaga agaaaaagaa ggttcaatct
    22441 tagtcccaag tactttcccg cgtgacaaag aacgaaaaga cattgcttat gacttctata
    22501 acttctcaga gggagaccaa agtagtgaag gttcttgggc tagtggcaaa gcactggatg
    22561 gcgaaggcga atatcaatgg ttgaaagaac ctaagatcga aggcgctgca ccaaaattag
    22621 aaccagtaac accaaaaatg tatgggacac ctccattcaa gaaataaata tctacccgta
    22681 gaaacacccg ttttttgttc cacacgcttt gtgagcaaaa ctgactaaag tcattaatca
    22741 taaccaccct tcaaaagggt ggttttctct agagctataa gctcaaatca ccggccagcg
    22801 cctaaagacg ctggctttca tattttatgt tcaagccata ttgcctttgc cagttttgcc
    22861 cacctcttaa aaggtttatg tactactcct tagcccttaa aaggatcgtc ttactctcgt
    22921 acactcagct tatctaacgc actatcatgt ttttcttgtt cttgaatgta ctttttgatg
    22981 gtcgcttcat tttgtcctac ttttgtcaca tagtatcctt ccacccaaaa gtgtcgatac
    23041 ccaaatttat attttagatt tgcacaatct cttgatgatc tcctgaatat tggctcgata
    23101 ttgattataa attatttttc ttctatactt tggtgtgaat acaatgtggt atttacacat
    23161 ctactttgta cgggctaaac tatttccctt tattgctcca cccgcatagc aagtggtttt
    23221 gtatttcaat cgctcggcgc gctcaattag gtctaaagac aataaatcta aaaacccgct
    23281 gcttcactga cagaaacaac gggttttcag aaaaaaatga aatgaaaaag atctcttacg
    23341 gcaatctatt ttattttttt gattttttat aaatggaaaa tgcttcaaga agtccaagaa
    23401 ataaagccgt ataaaaacaa gaacctatga aatctttatt tattagatat tcaacagaaa
    23461 tcccgataac actagccaac aatatattta tcagaagtgt aaaataagga tgctttttat
    23521 agaatagaac tagtgttgtt ttcatagact ctcctttctt catatttcca attaatcaca
    23581 aattcacggc attactagaa aaattaaagg ggcgcagaag tggaaaaata ctcttcgact
    23641 ttccctacaa accaggccat cttgtgttta ataactatac caaatctaag gtacctctaa
    23701 atctaaattc catttgtggc ggtgacctcc aatttttaac tcttctatct cccatccagt
    23761 atacataacc ttgatacttc gaccacttat atatcgaaca cacaaatacc tatccattgc
    23821 aggagtacta cctaacttag ttgctacttg catctaaata acagctgaaa agataggact
    23881 tactcttagt aaagtatctg aactattaat ttttaaacta tcatttttat cctcaactac
    23941 atgcacatct gttgcaactt cactttctgt ctcatccaga taaatgatta aggaatcggg
    24001 aattgcaata tcttcagctg acgctggaat agttgagcta aattagtcat catatcttta
    24061 ttataaaata accaacagca aaaaatacaa tgaaaaacaa acttggaact aatgtatcat
    24121 taataaatgt tgaaaaattc agcaactcat tactgtatga ttttagcagc ccaaccaaca
    24181 tataccctat aaaagctaat aagatggtta ggaatccttt agaattttta ctcattcata
    24241 gacacttcct taaataactt ttattataga aggtattgta aataataata ttgacaaaaa
    24301 gtgactcaaa ataaaaaaaa acttacatta tactggaatg aactgtattg atagtgtacc
    24361 taaaagctct actgacataa gatcgtgtaa tcggtaaagt aattttatta attatacggg
    24421 atgtaatcaa ttagagaata aactaatcat aaaaggtact tttacattca taattgtcgc
    24481 tttgagattc tactttggtt cactatctga cccaattgca cattttcgat cattacaagt
    24541 attggtgtcg ttagaacaac taaataaaaa ctagcggtag atttttcaga atctatgcta
    24601 atttctgtat ggatgttcca gtgatggcaa atttctcttt tcattgtttt tcttgtcgca
    24661 tgatatgcct ttttgtgtta ttcagttaaa ctatagaaat tctttcctag acagatcttg
    24721 atcccaaatt ttacgttttt gagcatcgat atagtttaaa atgacattac tcatatctaa
    24781 agagtttaga ttttttagga ctaaaactgt cagttgttct ttttcttctt tctgttaaag
    24841 tattgtcgcg acttttcgta tacgttaaga tatgtcgtga aaaaacaagt tttgtgacac
    24901 aaaaaaacga atgacttgag gaaatcacct caatcattcg tttaaaatgc tatatattct
    24961 ttagtttaag atacggaaat taatgccatc gaccacacta gtagaatgaa cgacatttac
    25021 ttggcctttt tttaatttcg ttatgtttag catataaaaa tattatagag tttacttaca
    25081 atgcttaaaa catatactgt ataatctgat caattttagc catgtcttgc aatggtaagt
    25141 tttctatttt tttgagtttt cgttccttaa aatcaagaga ttttaattgt gtaataatta
    25201 cttgtccttt tatatctagc tcttcaggta atgagtaacg tgtcggtaaa ttctttatag
    25261 tggaagtaat tggacatatg accgaaaaca ttgttttacg gttaaattca taacgagaga
    25321 cgactagtcc tggtcgtctt ttttgaattt cttttcctac ggagggatca aaatcaatcc
    25381 atacaatatc acctttttta ggaatataaa ttctttcttc tgacactata aaatttctct
    25441 cccttcagga gttaaatctt cccattcgtc tttctcataa tactcggcct cttccccacc
    25501 actaaatgga tcttcaattt ttggaactaa cgtaattgtt ccatcatcag aatacacaac
    25561 gatatattct tggttttctg aaggtttctt tccgttatca gaaggtaaag ttaccactac
    25621 agaacttcct tgtaatctag attttgtaac aagcattatc atcactccct taataaaagt
    25681 atactatata acactaagta acgcaaaatt cccctttgta aactatctag aaaattgttt
    25741 tgtttaaagt atatcttatt ggatataaat agaaataaat tgtagccagt tcattttttc
    25801 tttaactaaa aaaataatcc aagaaacatt gaacctatgg atccaaaagc tacccaaata
    25861 ctatagctaa ccccccgcta ccttcaattt tgcagataaa acgctttggg agaaaatttc
    25921 aaagacaaca ggccatccta tcatgcccga ttataaacaa aaagctggct attccttttg
    25981 ctctgacaaa tttcaattgg gaaagttcac gaaactagca ggtgaagaag tttcaactgt
    26041 tcggatcaaa gaatgcccgc ttcaaataga aacattggtt caacgaattt caactagaca
    26101 agactttgct attgtagaac gtgagatcgt agctattttt gtagaagaag ggattttaca
    26161 tgatccaact catatcaatg tggataaatg gcaacctctt atctataaat tcagagaata
    26221 tacaactgcc aaccagtcgt taactttgaa ttttagattc caagaattca aagagtcata
    26281 atgataaaca tcgttaggta taaataaaaa agactcgcag cacgagtaaa aaacaaatag
    26341 aagctataca ttagaaaggt cgactgtgac cctatagatg cagtatatgc agcttatgag
    26401 catttcttct gttctctttg tttttgggat ctgtgctgaa gtctttttcg ttttttgccg
    26461 ctctttcttt gaaaaatcga ttatgttata ccttctccat tactaattca ggcatctgct
    26521 gagtaatgat ttttcgcgca aaagaaatat ctgttaccca aggttcgatt tgttcttcag
    26581 tcaagacaag cggcattcgg tcatggattg gtgcgactgt gcggttaggt tcagtagtca
    26641 ttatgattga ttctgccatt ttttcatcct gatggctacg ataaaaaccg ccaatataca
    26701 ctattttttc cttatttgaa aacataaatt tgtctttttc ttcattccac tcattgggaa
    26761 aacacaacga ttttgcaaaa atggttgatg aaaggtcttt ttttcttcta ctgtctctgc
    26821 acgggcatta atcatcaatt gtcctttttt aaatccggta aatccccact tcatgatttc
    26881 cggagtgagg atgttttctt cctcttttct taacgtcaca acaagatttg acggaaaaac
    26941 ttctccagct gctaattctg ctgcctgctt cggttgtttc gtctgggctt ctcgataata
    27001 attctgaagg agctctgact ctagatcaaa aaaataacga ccacacatcc taatccctcc
    27061 caatcttttt ttctatcata cactgcaaca agaagttcgc accaacaaaa cgctttcaat
    27121 ttttgtagaa ctaaaaaagt tgtgacaagt gacaaactct ccagtgaatg gtattctcct
    27181 gccaactcct cggtcacaag tcacaatatt caacaaacat gagaagctgc tttttgaaat
    27241 tgttccttgt gactcggagt gaaacggcag gcttacgacc tctgggtaaa cggtgttcaa
    27301 aagccagttt ctcggtatta agccaaattt atgcaaaaat gtagaaagct attttcacaa
    27361 aatttttctt aatactcgaa actaagcgac tttttcacaa cctcttcctt aaacggtgtt
    27421 caaaagcttg catctgcggt aataagccca accaaacgaa aaatatgagc agctattttt
    27481 atttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc cactaaacga
    27541 tgttcaatca gctaaccttc ggtattagct caaaaaacgg caaaatatga ggaactattt
    27601 tgccgtttgt ttctctaata ctcaggtcaa agcgctgatt tcacaacctc taatacacgg
    27661 tgttccagaa gtagcttctt cgaaaaataa gccgaaattt cacaaaaatt tgagaagcaa
    27721 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctaattat
    27781 taaagaacct tttgcgaagt taacaatctt tctttgatca attgaaaatt ctgcctaagt
    27841 agtgatagat cctgtgtgac tttcaacaat aagatacttc cctggatttc agcaatcaac
    27901 gctttggctt gacggtctgc ttcttcatcg gacagttttt cttcagaaag tgccaggata
    27961 ttttgtttcg tatacttgac ccattgattg actaattcta gtagcaacag acggtattct
    28021 tcatccactg tactcaactc aataatcaaa ttacctacag gacagccatg ataagtgatc
    28081 tgtgtctcat gatcagcgaa aatccagtcc aacatttctt ctaatgcttc ttctggctgt
    28141 tttccagcat ttaagatgtc ttcgaacaat tctgtctgcc atacgtccat atgcgcatgg
    28201 atcaccgcta gacaaacttc tttttttgaa gaaaagtaat aataaaattg accttttcct
    28261 gtatcagaag ctgctagaat atctcggatt gacgtttcat gatagccttt tttataaata
    28321 agatctttgg tcgcttccaa gatcttttcc tttgttttca atgatattct cctttttcat
    28381 ttctcatagt cctatattat acagtaaatt catcattttt tcttcttaat cgtttaaaaa
    28441 agactgtgcc aaattatctg acacagccct cactataaaa ctgttctcat atagctagat
    28501 gttcattgat tcaagaactg cgaggacaaa agcaattcct atcttatgat agttgctcta
    28561 atacttcctc tgttgtcatc acttgatctg caataaagcc aaagttcgtc aacgctgcat
    28621 ccattgcttc ttggccagga gcaccaattg catctgtcgc taccacaaca ttgaatccac
    28681 tctctaccaa ttcacgcata tgggaatcgc tacacaagtt tccgttcatt ccaccaagaa
    28741 ttactgtgtc gattccattt tttctcaatt ggagcgccaa atcattgctt tctggtccaa
    28801 agattttatg aggagagctg atgatcgtat tttcatccaa ataaggtttc aattcttcga
    28861 taaaatcggc tcctgaacct tcttccactg attcatattg actttttcta gcaaacatct
    28921 gctgctcaag catcatatgt tctcctgcac cgccaaattg ccatttatgg tcatgtgggt
    28981 aaaaataatg aggagaaata aagagtttgt atcctttttc ttttgcttgt tttaccattt
    29041 caatcatatt ttcgatcgta ttgtattttt ctaagatatc ttttgtcaaa ccaaagccac
    29101 cgcctgctgg tttcaagaac tcgacttgcg ggtcagtgag aactactgct gtatttttat
    29161 tattgaacat ggtggattct ccattcgtta tttattttaa tacatcattt agagcttcac
    29221 tcattgtcgg atgagaatag atttgatctc tcaataacgt ataaggaagt tttccattca
    29281 tagctagcga aatcaagttg atgacttcat gagaatcttc cgcatagata cttgccccta
    29341 gaatcaagtc agtttcaggg tcgactaaaa ctttgaatac acctttagga tcttctaata
    29401 cttgtgcttt agggacaccg cttgtcatat acttgaataa tttgtaattg acgccttgtg
    29461 ctttggcttg tttttctgtt aaacctacat tggataaagt cggagtgata aatacactgt
    29521 aaggcacagc tttccggtta ttcgttgttc gtttattctc gcccttcaac tgatccataa
    29581 tgattcgata atcatctaga gagatataag tgaactgcaa tccgcctttg acgtctccaa
    29641 tcgcccaaat gttgtctgca tctgtacgaa ggtattcatc cacagcgatt gctccgcgat
    29701 ctgttagttt aacatctgta ttttctaatc caagtgatgc tgtacttggt tttcttccag
    29761 ttgcagccaa aacttttgac gcttggattg ttacttcttg gccatcttct gtaaaggtga
    29821 cggcagctgt tgattcttgg tctgctactt gatctacaga tacacctaga tggaattcga
    29881 ttcccgcatt tgtcatgtct tctaaaatca tttgcgcgat atcttcatct tcacgtggaa
    29941 gaaattcaga atgggcatca agaacgacaa ctttagatcc gtattcattg aacattgatg
    30001 caaattccaa gccaatatat ccagccccga tgatcactaa tttttccggt aattgttcct
    30061 gatccatcgc ttgcgtagaa tcaatcaagt agcgacttgt attcaatcct ttgattggta
    30121 aaatgattgg ttcagaacca gtattgatga aaatccgttc gccttcaaca atcacttttt
    30181 caccattatt cgtcacttcg atttgatggt tagaaagaaa acgtgctgta ccgtccatca
    30241 ctgtcccagt tgcctcatcg ctgatcatat gataattttt ggcgcgaagc atgcccgtca
    30301 atttttcttt ccgagcaaca gcctctgtaa agtcgattcc tctttctcca ttgaaaatca
    30361 atgatttaga aggaatacag ccgatgttga tacatgttcc gccatacata cgtgttgact
    30421 tttcaacgac taaaaccgat tcaccttttg aagctaacgc cttcgctaaa gtcttaccgc
    30481 cctttccaaa accgatcaca atattttgat atttttccat catttttccc tcgcttcttt
    30541 tttacctacc gattggtaca aaaatatgat aaccgataat tgtctattct ccaaatcatc
    30601 tgctttattt gtcaaaaaaa accagacaaa aagcggtcct ttcacgtttt tagtctggtt
    30661 cgatttcctt tattcttcta aacaatcgtt acttctacat cgatatttcc tctagttgct
    30721 ttggagtaag ggcagaattc atgcgcatct tctgcaattt tttgggcttc ttcttcgctg
    30781 atgccatcga tatgcacttc taatactaca ccgatctgat agccgccatc atctaaatta
    30841 taaagagaga cacgtgcttt tactgtggac tctgctgtca ttcctgcttc tttgatcata
    30901 tgttgtaccg ccccattgaa acaagaagca taacctgctg caaataattg ttctggattt
    30961 gttccttctg catggattcc tggaggagtg attttcatat gcatcgagcc atttggtgcc
    31021 tcgacttctc cattgcgccc gccatggttg atcattgctg tttcgtatgc ttttttcatt
    31081 gtactgctcc tttcgattga ccatttgcta cttggctata attatagtgt atgacaagta
    31141 aggcgctttc tacaaaaatt ctgcttattc gaataccaga atgccgtctt ctactatttt
    31201 tccaaaaaac aaaaaaatac tactcgtgaa caacagcgtc atatttgttc caaaataaat
    31261 agcaatcttt tctacataat cttttatatg attgatttta agatacatat ataccagaaa
    31321 gttgacccca taaaccaata ctgttaaaaa aataaccata ttcgttacca ttttttcctc
    31381 cttttgctgg tctccctacc agcactcgat ttaaccatac cacttttttt gccgtccaag
    31441 caactagcct ttcaaaaaga ctggtttatt ctgctgttaa tgattcaaga tcagaaaaat
    31501 gtccgaataa ttttgctaat tttactaacg ccaacttgtt ctttccgaat tcatcatttt
    31561 ctatctgtaa tactagtaac ggctcatgta agcttaaacg gatcaaaaac cagccgtttc
    31621 cgtatggctc ttctagatca tatcgaatac cttcttcatt ttccgggtcg aagttcatcc
    31681 ctttttcttc ttccaacagt cgcttcattt cttctaatat cttgtttcct tgttcgcgtg
    31741 gttcatcgct gaacaatttt aatcggattt cttgtacttc tacgggctgt ttcaactctt
    31801 tgatttgttc atctaatgtg ataccttttc tttttaattc tggcaaaagc atcaggatct
    31861 ttgcgatgac atatgcacca tcatccagaa aatagttttc cttgaatgca gcgtgtccgc
    31921 ttgtttcgat ggctagctgt gtatctttcc caagacggtt aagttctaat gctttattga
    31981 ttacatttcg atagccggaa atataacgaa cttggatgcc tcctttttct tcaatgaatc
    32041 tttgtagatg agaagaagta ggggaattcg tcacgattct tgttcctgga tgctctttta
    32101 atacgattgt acttaatact gcgatcaaat tatttcgatt gatcactgtt ccagaagcac
    32161 tgacaactgc agaacgatcc acatctgtat cgaaaatgat cccaagatct gcttcatttg
    32221 ctaaaacagc ctgcttgata ctttccatcg cttctttatt atcaggattt gggatatgat
    32281 taggaaatcg gccatctggt tccaaaaatt gggaacctgt tgaatccgca cctaagctat
    32341 tcaatacttt ttcagcaaag aatccaccag caccatttcc ggcatcaaca atgattttcc
    32401 aaccatttag tggtttgctt tctagtgttc caatcccctt tttaattttt gtgactaaat
    32461 cttgcgcata aggtgtcaaa aggtctgctt tttctacgct tccagtttgt tcgcttttta
    32521 ttttttgctg ctgtgatagg ataaaagcaa tatcttcttt ttctgctcct ccttctgagg
    32581 taaagaattt tattccatta taataatagg gaagatggct agcagtcagc ataaccgcag
    32641 catcgcacga gaactgttca aattgagtgg acataaacat agcaggcgta gtagctaatt
    32701 cgaaatcgat gacatgtatc ccttgttctt tcagactttg gatcaacgcc tctttcagtg
    32761 ctggtccgct tatcctactg tcatgaccaa tcccaatcgt ccatttttta tttggatcgg
    32821 ttgatttctt ctttttcttt atccagtttg ctagtcccca gccgatcttt tttaccatgt
    32881 ctgtcgtcaa gtttacttca tatccttcaa ctggtaatgc gattcctcga atatccgatc
    32941 cattttgcag cgctttcata tctttcatat aaaagtcctc ctcatttatt ccctcatttt
    33001 ttccagcttt tctgcaaatc gctgtgcttc ttcttctgag cggcaaataa taatgaatgt
    33061 atctacaccg gccatcgttc cgacaacttc agggtaagcc acttcatcta gccaattcgt
    33121 taccacatcg gcaccatttc gttcagtcaa tacgataacc atgaactgaa cagactggat
    33181 acgcaaaacc tcgcgtctaa ttgctgtacg caaacgttct tcatttaaag aaacagatgg
    33241 ctggttaaat aatgtatagc gtactatttt gttttcattt tgacttttta ctaacttcat
    33301 ttctcgaatg tctcgagaaa tcgttgcttg agtcacatcg gcgcctgctt catgcagccg
    33361 cgcaagcagc tcttcctgtg tcccaatagg ttgttctgta atgatctggc ggatcagtgc
    33421 ttgtcgctgt ttctttttca tattagacac ctctcaatgt tgattatagc aaaagataag
    33481 taatatttct tcatattttg gtatttttta cttttatttt ttgttttcat tcaggttgct
    33541 aacctttttc tattttaaca cgcaaaatcg tcttcatttt ttcaggtgct gattttcttg
    33601 gatcgctgat gtaaatctct ttgtgttctt tagaagctct ttcatagcct tcttttgcga
    33661 tgaatgcttc taatttctca aacgttgctg gttcttcggc atagggcccg acgtgtaaga
    33721 tttgggcaca aagaccttct tccatctttt tgaatactac ttgttctttc aagtcttccg
    33781 gaagtttgct agaacgctga atggcttctt gtgctatcgc ttctgtaaca aagtcaggct
    33841 gtcggatcat caattgataa gaaaaatgtt cttttttcat cactggttca tgcaaatact
    33901 tttcttgcag tccccattgt ccttccagtg gatagacagt atacatctga taatcaggaa
    33961 tcagccaatt ttttttaggc gacatgcgta ttgcatagct gactgcatat agacaggcta
    34021 ctcttcgttt aaagtcttcc tgattcgggt cgccgatccc tctgatgcta aagaatgatt
    34081 ttttaggaac ctctagtatt tgcgggcttt ttttcatact atagctcttg tctttacgcc
    34141 attcaatttt ttccattttt cttcctccgc gaaattccat ttagttattt attatcatac
    34201 ctctttttga gtgacttggc taatcgtgtt gacactattt ttcagtttgt gctagaataa
    34261 gtgaagtaaa taaatcgtac agataacacg tttcggcgtg tcgagagaga ggcggtttct
    34321 atttttataa tagaaatcac tctctttttt tacgaaattt tttagaggtg atgtaaatga
    34381 caaaacaaaa acagctgctt ttagtacctg cagtaattgc aacagggatg atgtcttttg
    34441 cgggcgtatt gatcgagacg gccatgaatg taactttccc aactcttatc agacagtttg
    34501 atttaacgac tgcacaagtc caatgggtaa caacgattta tcttttgatg atttcgatca
    34561 ttgttccgct gtctacttat ttgaatcgca attttagtct caaaaaactc tttttagctt
    34621 caaatctgct tttcttggca ggtataatga tcgatttctt ctctcctacc ttcatggtcc
    34681 tattgtttgg tcgattgctc caaggtgcag cgacaggtat cgctcttccg ttgatgttcc
    34741 atattatctt gacatttgcc cccctcaatc gacgaggcgc gatgatgggt gtcggtactt
    34801 tgacaacggc cattgctcca gcaattggcc ccacttatgg aggcattatg acttcacatt
    34861 ttacatggaa tcatatcttc ctgtttcttg tgcccgtttt gattctttct ttgattatcg
    34921 gctggtttgc tattcccgag atggcagtac aaaaaagtgg ttatttggat tggataagcg
    34981 ttttaggaat ttgtcttttg tttagtggaa gtctaacatt tttgagtacg ttgggaacag
    35041 tttccagctg gattgcttta atcatcggcg gtattggctg gttccttttt tactatcgaa
    35101 gtaaaaaagt ggagcatcca ctgatccgac tgtctattct ttcaaataaa acattccgct
    35161 tgtttttgat tggcttctta gtttatcagt ttttattact tggtgtatct tttgtcttgc
    35221 cgaatttcct tcagatcgtt caaggaagta gtccttttgt cgctggatta gcgatgcttc
    35281 ccggcgcagc ggttggtgct ttgctttctc ctttttccgg gaaaatgctg gatcaatatg
    35341 gtccgaagaa gccaattttt gctgggctga ttctttcttt gattggttgg ctagcattat
    35401 tcctactcat aggacacgct tcagtgggtg tattgattgc ttgtcatgtg ttttatatga
    35461 tcggtatcgg attatcttac agcaatatga tgaccaccgg aatgaatgca ctgaatgaag
    35521 aactacaagg agacgggaac gcaatgttca ataccttgca gcagttttct ggcgctgtgg
    35581 ctacttcatt agtagcggtc attatcaatt acgtccagca tcatacttcc cataactatg
    35641 aagtgtctac tacattagga tcaaaagcag cattaggcgt cttgttgctg ttgcttctcg
    35701 taagctttgc tcgattcgcc tattatctgt tctttgctaa aaaagcgtag attccatata
    35761 cggcaggtgt tgcatctgcc gtttttttgt gtgaaaatgg ttgtttgtaa aaggaggctt
    35821 ttttatggaa aaaattgcca ttatcggtgg cgggatcatt ggtatgaccc tagccagtta
    35881 cttagataca tcaaaattca atgttgcttt atttgatgaa gggacaggac aggcaactaa
    35941 agctagcgct gggattatct ccccttggtt gtcgaaacgc agaaacaaac aatggtatcg
    36001 tctggctaaa gacggcgctg cttttttccc taaacttata aaagatttcc aacttgatca
    36061 aacgatttac gcacaatcag ggacactttt gcttcgacct gaaaaagatt tagctgaatt
    36121 ggctgttctt gccgagaaac gaaaagaaga agcgccagaa attggaaaca tcgaatggct
    36181 ttcacctgaa cagacatctc aagcacttcc gttattgaaa ccagcagttt cactgcgtat
    36241 cagtggtggt ggtcgtctgg atggaaaagc atatttagaa catcttggtc agcgtttagc
    36301 acaaaaacaa atggagcaga tccaggcaaa agtcactttg cataagaaga gcgattcttg
    36361 gctcatcaag ggaccgtttg gagaatacac agctgatcgt gtaattttga cacctggccc
    36421 ggcacttaag cctttattac aagaattagg ctatgctgca gatatacgtc cgcaaaaagg
    36481 gcagctctta gcatttcaga catcttataa gatgagtcaa gactggcctg ttgctatgct
    36541 tgacggagaa gctgatttca ttcctttcat ggatggaaag atccttttag gtgctaccca
    36601 tgaaaacgat caaggctggg atttggctcc tactagcgaa gcttataagg gattgactaa
    36661 gagtacagaa cagttcttga ctgatccaga tcagataaaa aactggccat acgagttccg
    36721 tgtaggtacc cgtgcttata cgtcagattt ttccccattt ttcggcgtac tgccagatga
    36781 tccaagctta ctggctgcta gcggtctagg ctcttctggc ttgaccacag gtccttatat
    36841 cggctatcta ttagccaact tcttaaatac tggtcattgg cctacggaag attatcaaaa
    36901 ggacatctcc acctatatta cgaaaaatcc atcatagaaa aacagctttt tcaagtaagg
    36961 atcccgagcg atattgcgca tggattactt cttgaaaaag ctgttatttt agttcaattt
    37021 ctgaatcaaa ttctccaaac tttcttttgc ttcatctggc aagagatctt ttcctatctg
    37081 agcttcaatg aatcgtaaac gaatatcaag tcgttgtttc accagatttt tataggacgt
    37141 atcctcaaaa gaaacaggaa aatctggtat aggtaaatag tggatgtctt caaatggacc
    37201 aaacgacttg aattctgccg tttgttgcac gattctttca acgattgaca atgtcgtttc
    37261 aggtttgatt tttttatcat cgaaatatcc agtattcagg acatagcagg ctgtttgctg
    37321 tttcaaaaac aactctcgaa aagcttgata gtcttcccta agaggataga cacggaaagg
    37381 attagcaaat ggttcaatga ctagctcatc tagctgtctt tttgtagtga tattttccgc
    37441 gtctgatcgt tttgttgcta atgttgcacc aaaagacgct gctaaaactg ggtcattgat
    37501 cttgataatt ggcggaagcg aatcatcttt catgatccaa aaaacagcat cgatcttttc
    37561 gctaagatga tcgactcggt cgggtgtcgc ataacgtgat ttgactgtcc gcccatttcc
    37621 gtttcgtata tctcctgtta ctaggacttt ttctccttta cttgtacatg tcacgcccac
    37681 attctgacaa gtaaggaaag aagaaatggc cggatcatca gacggatagt cttgtgtttt
    37741 atcaaagtaa gaaggttcca aggcagtagc aaaaccattt tttcgagaaa taacgaaagc
    37801 atcgtcatgc aagatcgaaa cagagtgacc ttccgtatgt tttgctaagg taatcgttga
    37861 ttttccagaa ccggacaacc caaatacagc catcgtatag gtctggtcat tactagcata
    37921 ttgtttcatc ccaccgtggc aggcaataaa gccattgcga tttcccaatg cccaagcaag
    37981 cgtcaaagtc gcttttttta attctccaaa gtaacgtaat cctagcacag ccgctgtatt
    38041 atggagagca tcaaacaaaa tgagtccatt tggaaatgtt tcatgtgtcc attcgggatc
    38101 acaaaagata taaagatctc cctcatcaaa ggaaacagac ttttgataag tctctaagat
    38161 cttcgaattc gctggctgaa aattcagcaa ataagaatac aaattgttct caaatccttc
    38221 tggaatcagc aaatgacttt gcacagaaaa ttcttcagat aggccaacga ttacttttgt
    38281 atggtaaaac tggcgcttgc gactttgata cacagcttct cttaaaattt tctcgtattt
    38341 gctttgttcc ttgtctaagt cctcaaagat ccgccgagca cttgctgttc ttcccacaat
    38401 gcttccatca ttgaatacta gtgtcctaac atcagctggt aaatctagtt cttcagcatg
    38461 tgagattgtt aaatctgtga tgatcgttcc tggactccct tttgctagtt gataagctgt
    38521 cttcctatca aaaacttcat gaacattatt accgtaaaat gtcgtttcta caatcgatcg
    38581 aatactggat aacagtggat ttccacttgt gatttcgttt aattcgagac tctcttttgt
    38641 actcatggct cctcctcatt ccctaaacaa aaaaacgttt tctttattat acattattcg
    38701 agtttttatt tgtactgctg cgctgtggga agcaaacgag ttatttttca cacaaaaaaa
    38761 ccgagaactt gaataaacaa gtttctcggt ttcatcattt tccttttatc ggcttagtac
    38821 cagccgtttg ctaaccagaa tgttttagca gcatcccatg aaccataacg agatgttaca
    38881 tattgttctg ctacacgttc ttgattggca gctgaatagt cgccgtttaa gtatgaagca
    38941 tctaattgat aacggccgat gtaacgtccg ttagtagctg tgtatgaacc gcctgattct
    39001 ttttgtgcga tccattcttt tgcagagctt gaagttggcg ctgttgcagc agctggggca
    39061 gcaggagcta cttcaactgt ttcaacaggt gcaacgtatg tatgttcttg cgttgctgtt
    39121 tcagttgtag atgctgtatt ttctgttgtt ttgttttctg ttgttgctga agaatcattt
    39181 gaagttggga ttgttaattg ttcaccaaca taaatgcggt tgatgtttgc gatcgaattt
    39241 ttttcagcaa ttgcatcgat cattgagttg tctcctgcaa atttttgtga gatcttagat
    39301 aatgaatcac ctgattttac tgtataaact tcatcagcgt gagcatttgt agttcctaca
    39361 aacattgcag tagcaccagc agcaaaagtt gtacctaaaa caattttctt aaatgaattc
    39421 atattaaact cctttttttc tccgtttttt attctttcga cgctattaac t
//
LOCUS       DACESX010000010        39189 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.010, whole genome shotgun sequence.
ACCESSION   DACESX010000010 DACESX010000000
VERSION     DACESX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.010"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            64..384
                     /locus_tag="IXF88_000441"
     CDS             64..384
                     /locus_tag="IXF88_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1507197.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(496..1365)
                     /locus_tag="IXF88_000442"
     CDS             complement(496..1365)
                     /locus_tag="IXF88_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1507198.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(1461..2765)
                     /locus_tag="IXF88_000443"
     CDS             complement(1461..2765)
                     /locus_tag="IXF88_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1507199.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(2762..3271)
                     /locus_tag="IXF88_000444"
     CDS             complement(2762..3271)
                     /locus_tag="IXF88_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507200.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(3446..5581)
                     /locus_tag="IXF88_000445"
     CDS             complement(3446..5581)
                     /locus_tag="IXF88_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1507201.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(5664..7319)
                     /locus_tag="IXF88_000446"
     CDS             complement(5664..7319)
                     /locus_tag="IXF88_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1507202.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(7321..7635)
                     /locus_tag="IXF88_000447"
     CDS             complement(7321..7635)
                     /locus_tag="IXF88_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1507203.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            7805..8065
                     /locus_tag="IXF88_000448"
     CDS             7805..8065
                     /locus_tag="IXF88_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1507204.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            8079..8393
                     /locus_tag="IXF88_000449"
     CDS             8079..8393
                     /locus_tag="IXF88_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1507205.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(9123..9677)
                     /locus_tag="IXF88_000450"
     CDS             complement(9123..9677)
                     /locus_tag="IXF88_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1507206.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(9803..11732)
                     /locus_tag="IXF88_000451"
                     /pseudo
     CDS             complement(9803..11732)
                     /locus_tag="IXF88_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(12038..12754)
                     /locus_tag="IXF88_000452"
     CDS             complement(12038..12754)
                     /locus_tag="IXF88_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1507207.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(12755..14578)
                     /gene="glpO"
                     /locus_tag="IXF88_000453"
     CDS             complement(12755..14578)
                     /gene="glpO"
                     /locus_tag="IXF88_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1507208.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(14592..16088)
                     /gene="glpK"
                     /locus_tag="IXF88_000454"
     CDS             complement(14592..16088)
                     /gene="glpK"
                     /locus_tag="IXF88_000454"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1507209.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(16224..17684)
                     /locus_tag="IXF88_000455"
     CDS             complement(16224..17684)
                     /locus_tag="IXF88_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1507210.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(17674..18969)
                     /locus_tag="IXF88_000456"
     CDS             complement(17674..18969)
                     /locus_tag="IXF88_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1507211.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(19301..19978)
                     /locus_tag="IXF88_000457"
     CDS             complement(19301..19978)
                     /locus_tag="IXF88_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1507212.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(20008..21099)
                     /locus_tag="IXF88_000458"
     CDS             complement(20008..21099)
                     /locus_tag="IXF88_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1507213.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(21200..21634)
                     /locus_tag="IXF88_000459"
     CDS             complement(21200..21634)
                     /locus_tag="IXF88_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1507214.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(22218..23675)
                     /gene="ascB"
                     /locus_tag="IXF88_000460"
     CDS             complement(22218..23675)
                     /gene="ascB"
                     /locus_tag="IXF88_000460"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1507215.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(23672..25567)
                     /locus_tag="IXF88_000461"
     CDS             complement(23672..25567)
                     /locus_tag="IXF88_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1507216.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPSIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(25625..27400)
                     /locus_tag="IXF88_000462"
     CDS             complement(25625..27400)
                     /locus_tag="IXF88_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1507217.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLDESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(27450..28946)
                     /locus_tag="IXF88_000463"
     CDS             complement(27450..28946)
                     /locus_tag="IXF88_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1507218.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(29140..30762)
                     /locus_tag="IXF88_000464"
     CDS             complement(29140..30762)
                     /locus_tag="IXF88_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1507219.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(31090..32403)
                     /locus_tag="IXF88_000465"
     CDS             complement(31090..32403)
                     /locus_tag="IXF88_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1507220.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(32648..32872)
                     /locus_tag="IXF88_000466"
     CDS             complement(32648..32872)
                     /locus_tag="IXF88_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507221.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(32892..33260)
                     /locus_tag="IXF88_000467"
     CDS             complement(32892..33260)
                     /locus_tag="IXF88_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1507222.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(33457..34167)
                     /locus_tag="IXF88_000468"
     CDS             complement(33457..34167)
                     /locus_tag="IXF88_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507223.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(34185..34682)
                     /locus_tag="IXF88_000469"
     CDS             complement(34185..34682)
                     /locus_tag="IXF88_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1507224.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(34719..35171)
                     /locus_tag="IXF88_000470"
     CDS             complement(34719..35171)
                     /locus_tag="IXF88_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1507225.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(35306..35911)
                     /locus_tag="IXF88_000471"
     CDS             complement(35306..35911)
                     /locus_tag="IXF88_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1507226.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(36361..36981)
                     /locus_tag="IXF88_000472"
     CDS             complement(36361..36981)
                     /locus_tag="IXF88_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1507227.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(36983..37852)
                     /locus_tag="IXF88_000473"
     CDS             complement(36983..37852)
                     /locus_tag="IXF88_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1507228.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(37875..38279)
                     /locus_tag="IXF88_000474"
     CDS             complement(37875..38279)
                     /locus_tag="IXF88_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1507229.1"
                     /translation="MLQSAKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEISTT
                     QDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRADA
                     ARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            38358..>39189
                     /locus_tag="IXF88_000475"
     CDS             38358..>39189
                     /locus_tag="IXF88_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1507230.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT        11114 a         8317 c         6596 g        13162 t
ORIGIN      
        1 ggaaaatatg tgtagtgctt cttgaagttt gttatacttt aattaaaaga gaggagtgat
       61 cgaatgatta caacaacaac gaacagtgta gagggaaaag ccgtagtagc ttataaaggg
      121 atcgtttttg gtgaggtcat tactgggatc catgcattta aagacttgga ggcggggttg
      181 cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa
      241 gaagcacttg ccgaaatggc cgaacgtgca aaagcattag gagctgatgc gatcattggc
      301 gtaaaaatgg actatgaagt attaggcagc gacaatggca tgctgatggt cacatgtagt
      361 ggaacagctg tctccacgag ataagtaggg gacatctgtt ttaataacta aagagagatg
      421 ggacatgtag tggcactcaa gaattagatt tttcagtcta acttcgaggt actctacctt
      481 cccatcctct cttttttatt tctttttgcg gatgattccg taagctgctg cgactaacac
      541 acccgcccct gctaaggaat aactgacaga agctgttttt tcatttgttt ttggcaaagt
      601 ctgacttgtt gatttggatg gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga
      661 ggtcactgat ttttctcctg ccgaatgcaa aatatctttt attcgttgga aactagctgc
      721 taagtcagct ttttttgctt caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc
      781 gtggattgct tgactggccg ctgtgatatc cgcttgagtt tgacttagct ttccagaagc
      841 tgtttgagca acctgattta attgctgatt gaattctttt gcttgttgga tcttcccgtc
      901 aagatctgtt tggacagctt ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg
      961 tgcacggtca tttcccactt tatttttaag atcagctaaa tcttgatcga ttgctgcttg
     1021 gtcttctttc aatttgtttt ttgcagcttc taatgcttct tcgagttctt ttcttttatt
     1081 ttccaagtct tgcaaatgat cgatttggtc agaagaggta gaactattta ttgatgtatc
     1141 ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt acattggaag tcatttctgt
     1201 ttccgaggca ccgttttcag caccagaaga gcttatggtt gttgaaggtt ctgtttgagc
     1261 aggttcagaa gtttccgcaa aagctgtctg tcccaataag aaaatactgg aaacagcaat
     1321 tggccagatt aacggcttca ttcttttttt catcactttt cccatcctta ctacttttta
     1381 actaatttct tattataagc aaaaatgttt ggaagaacca taaacacaac aggctgtttg
     1441 tctaaaaaac ggtgcatctt ttattttttt gtacgttccg ggcttttcca tgtcccgctt
     1501 ccataaccta aaatcgtttt gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc
     1561 actatccaga acaaaagcat atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt
     1621 cgatcatcta caatcagcga agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag
     1681 atgaacacaa aactgaatgt aaaagtaata taaatctgtt cgtagtttcc ttgatataca
     1741 taatgcccga ttaatccagc aaaaagaact acggaaatac aaaagaatat tgaccagaca
     1801 atactcaatg tttgatcaat gaagatgatc gttcttccaa tatgtttaaa aggatggagc
     1861 atcacttttt tgaaattcgt cagccagact tctgttccac cttttgccca acgttttctg
     1921 tgtcgatata gcatcttcaa cgtcacaggt acttccatga aaaagataat ctcaggagca
     1981 aaaacagata accagtcatt taactgatga tcccaagcaa tactgatgtc ttctgtcgct
     2041 cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt ttcgatacat cgtatttgct
     2101 ccactgtacg cataaagacc accgaaaaca gctgactgtg ttcgtttaat gatgcccacg
     2161 atacttgaga attcaaccgt ttgagatttt gcaatcaatt tggtacgatt ttggacatcc
     2221 atatttgcag tgaccgcagc aatatgtctt gcaccagggc gaatgaaata attcacatac
     2281 ctaatcagtg catctggctc cggtaccgtg tctgcatcat tgcttaaaat caatttccct
     2341 tttgcaaagg caagtccgat attaaacgcg tgggcttttc ctttattttt ttcgatccga
     2401 accacacgaa gattagcgta ttttttcatc agtctagcta atatttctgg tgtttggtcc
     2461 gttgaaccat cgtctgtcac taatacctca tagttgtgat aattcaattt cgtcatcaaa
     2521 tactcgattg tatattcgat cacaatttct tcattatggg ctggcaccat gatcgtgatg
     2581 aacggttcga cactcaaggg aacatctacc cattcttttt gcttatgttt gtataaaaac
     2641 acataacacc agacaccaat aaaccataca aatccgccta aaatcggata agaaacgaga
     2701 aaaaaataca taatagacaa acaagtatct aagaataaat tacctgtatg ggtgattgag
     2761 atcatttcag ttccacccca ttttctttat acaaatctgc aatgaatgtc gtatctagat
     2821 ttttctcttc ggagacagaa taaaaacgga ccgtttctct ctcttctttt gttccaaaac
     2881 gttccgtata cacttcttca agtaattctt gtcttttttt caatttttca tcttcatatt
     2941 caaaagattt atttactttt ttgtatagat ttcgattgtt ccacctagtc aatacaaatg
     3001 aggtaatcag gaaactgaaa aaaatcaata caaaaaagcc gattaagaaa tacagcattt
     3061 tcttttctga taaaaaagcg tacaaggaaa aatccttggc tctttctggt ctcgttaatg
     3121 ggaaaaagac ccatataaaa ggaagaacaa cgatcatcca tcctaaaaga gccactaacg
     3181 tttgccaaat ctttaaccag taattacctt ttgaaaaata cttatctttc accaaaattg
     3241 atgcaacttc ttctcttgtt acttttttca tatcctcacc tcacgttttt ttgttacgac
     3301 gtaggagttt accatacatt tcatttcttt tcacataaag tgttcctaaa tttttcctaa
     3361 gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata ttttgttctt tttatcagtt
     3421 gttcttttta ttttttatct cttaattatg ttgctctttc caatacgaca atgcttttca
     3481 ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt
     3541 caaatggata taagattaca tgatcaaatt ccagtccttt tgctagactc aaaggaatga
     3601 tttggcagtt ttctaatcca tatgaatttt ttttaatcga ttcttgtagc gtttgggttt
     3661 cctcttctgt ttgggtgata actgccgctt tttctccttt tagagaaacc aaaatctcag
     3721 ctagtatttt caacaattct tcagaggatc gaaaagagcg gtattcgggt tcttttcctt
     3781 gcggtcggat cgatacgatg tcgatcttct cttgatccac agcataggtt ttgaaaagct
     3841 ctgtgattgc tccgctagaa cggtaactat ttctaagatc ataccgatgg atcggcagat
     3901 ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt ggaagaatta aagattgcct
     3961 gattctcgtc acctactaaa gtaaagtgag tttttggaaa taattcgatc aatagaccta
     4021 attgtgccag ggtataatct tgtacttcat caatcaatag atagcgaaat gctggtacat
     4081 ttaccttttc aacaagtaaa tgacgaatag ctaatataat gactgcttca tcaacagtac
     4141 gtgtgggttg ccaagcataa gctgagccat atcgtttttc ataaattctt tcaaacaact
     4201 gatgctcatc cacccatgcc atttcttcga tttgtctggt tatttttcga tatttttttt
     4261 gtaaaagttt ccttgcataa gctacaatac tttgttccga atcatcagaa atcaatttac
     4321 cgaataattc caactgccta tcttctgtca acgagagtac ctgattctgc acagcaggac
     4381 tttttgcttg tttcaaaaga tgattcttcc attcgctcat cagtgccttt ttcgttgctt
     4441 gaagcctatc gatcaattta ggatgagcag gaactttctc atatattttt tcgatcgttt
     4501 ttttagaaag gactgttttt ccatttttag taagtccacg gataaaattc ggatgatcta
     4561 aaaacaaagg atctgattgt tttagattat cgataaactt tttgctacgc aaacgttctg
     4621 tctgctccga tacattttct cccgtgatac gtttgaaata agcctcttca ccttctattt
     4681 cctctgctga aagctgtgaa accagctgta tgatggttag attcagtgga tttctttcac
     4741 ctaatgatgg taggacattc gaaatatagt cgataaaccg atgattaggt gatagaatca
     4801 gcaagtcatc ggatgtcatc gtatctcgat ataaatagag cagatacgca atacgctgca
     4861 tgatagcaga ggttttacca cttcctgcta cgccatttac caagatattt tgacttttca
     4921 catcccgaat gattgtgttt tgttccgatt ggatcgtagc agtgatagcc ttcatttcat
     4981 tggtctcatc ttggcctaag acttctaata atacgtcatc ttgaatagct acagacgtat
     5041 caaaaaactt gaccaaacga tctctttcca aaagaaattg tcgtctttct ttaataaccg
     5101 tatcgatttt attttgacga accgtataag aagatggacc aatcatgtta ttgtaaaaaa
     5161 gttcggcaat tggtgaacgc caatcataaa caagattttc atctgcatca tctgtgaaat
     5221 ttgctgtacc gatataaaag gattcttttg gctcattatc cagaaagtct acaacgactt
     5281 ttccaaagta gggacttttc aataaacgtt cgatctttgc tagctgattt tctgcgtttt
     5341 tctgtttgat attcatctga tcgatttctc tatttttcat ttcaatcatc gaaaaagtat
     5401 ccaggttatc taagtaactg tcaaaattca atcgaagctc gccagccatt tctttcaacg
     5461 actggtttcc ttctattttt gccgtatcca tcgattcccg cagttcgttt tttttatcaa
     5521 ttagcttttg atacacgttc ttcagatatg cttcttctaa ctgtttttgt gtgttgacca
     5581 tacacattct cctctttttt taaagtggtg gaaccgatta gtttacctct tttaagagtc
     5641 gaatgcaagc tttttaacga gtatcatttg ttaggatata tcagttcttc aggtcggaca
     5701 gtttgatata aagaaaatgc accatcaagg ttttttacgt ggtagccaga ctgcttcaag
     5761 attctttccg ctagatagct acgtaatccg ctatgacagc tgacgatata ttcttgttgg
     5821 ctgtctaatt catttaatcg gtcccgaagt tcattcaaag ggatatgttt ggcttccttg
     5881 aatttgccct tttccaattc ttctgctgtt cgcacatcta ataaaatttt tccttttgct
     5941 agttctgtcg gcaattcatg ccattgtacg gtttcactaa gcccttctgc aatgttcatt
     6001 gctgcatatc ctagcatgtt tactggatct ttggctgagc caaatggcgg tgcatacgtc
     6061 aattctaatt ccggtaaatc gaagatcgtc aaatgccctt tgattgctgt tgctaaaata
     6121 tcgatccgtt tatccacacc ttttgcccca acaccttgtg caccataaat ctcgccagtc
     6181 tctggatgga agatcaactt cagtaaaata tctgttgctt ctggataata gcttgcatga
     6241 tcttttcctg atacatgaat gacttcaaaa gacagttcgt tttctttggc tgttctttca
     6301 cttaaaccgg tggatgcggc agtcattcca aacgttcgta taattgctgt tccaataccg
     6361 ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg
     6421 gctggtgaag caagagaaat aagtgcatct tttccagtga tttgctgttt tacaacgata
     6481 gcatccccca ccgcataaat atcaggaata ttcgtttgat aacgttcgtc tactaggata
     6541 cccccgcgta tgcctaattc gattccagcg gtcactgcta gctggttatc aggacgaaca
     6601 ccaatggata aaataactaa atcagcaggg atggtacgcc catcatccag tcgcaatcgt
     6661 ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa tcacttctac tccttgatga
     6721 gacaattctt tttctataaa agctgccatt tcttcatcca gtgttggaag aatatgtggt
     6781 gcttgttcga tcaccatgac ttctaaacct cttcttttta ggttttctgc catttccaat
     6841 ccgatgaatc ctgcaccaac gatcacggca cgctttggct gtttttctaa tgcatgcatc
     6901 actttatcga tatctggtac atttctaata gaaaaaacat tgtcggcttc tgttatccct
     6961 ggaatcgatg gaacaactgg ttttgcacca ggagataaaa ccaatgcatc gtactgttca
     7021 atgtgttttt gaccctcatg tacgacttct accgttttgt tttcgggaaa gatggctgtc
     7081 acttcatgat ttggacgcac atctaaacaa aaacgagcgg ataatgattc tggcgtttgg
     7141 acaagaagat tttcccgttc actgatttct cccgaaagat aataaggcaa gccgcaattt
     7201 gcaaaagaaa catacggtcc tttttcaaaa acaacgattt ctgcatcttc cattaatcgg
     7261 cgaagccgtg tagcagcaga cattccacct gctacacctc cgacaataac gattttcatc
     7321 ttatttccct cctactgttc gatcaaacca ttgattcatg ccaccacgta cattcactac
     7381 atcataacca gatttcttta attctttagc tgcttgttta ctcctcatcc ctgattgaca
     7441 aatcacatag acggtctttt ctttgtctcc atcatatctg tttatacttt gtaatggaac
     7501 atttttagct ccttttatat gcccgccccg gtattctgca ggtgttctaa catcaagtaa
     7561 cgctggattt tccaaaagct tttcctttaa ttctgggact gttatcgatt ttattgattg
     7621 aaatagcaaa ttcattttct gcacctcatt tacattttta taaatacctc atggggtata
     7681 ttaaacagga gctttattaa tatgtcaacc aaaatgactc ttcatttttt tcactacttt
     7741 ttatatttgc ataaaatacc gataaggggt attatctaat tataaaaata gaggagaatg
     7801 tgttatggag attgataata aaaatatcct gaaccgtttg aaacgtacag agggacaaat
     7861 tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt atggatgtca tcactcaatt
     7921 aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa
     7981 aaattgcttt gagaaacctg atagtaatcc agaagaacaa gcggctaaac tagaacaagc
     8041 gatcaagatg attattaaaa agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg
     8101 atgaattaga aaaatggtca gaaacaggga aaaacgtaaa aaacctgata aaagcttctc
     8161 cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat taccggttat ctagactctg
     8221 ccaatgcaga attcttagat ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc
     8281 gtgctaatca tatttcagaa agttctttac ctgaacaagt aatcgttgta ccagcaggtg
     8341 tccttgattt agttgaactg caatcccaag gttatgctta tatcaagcct tagtcagtag
     8401 aggttgcgac agaagtgatc agcttcaaga aatttcggct tatttttcga agaagctact
     8461 tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc tgcatcaagt aataagaaca
     8521 gccaaacaaa aatagcttct catatttttc gtttggttgg gcttattacc gcagatgcaa
     8581 gcttttgaac accgtttaag gaagaggttg tgaaatcagc ggtttgacct aagtattaga
     8641 gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt ttgagctaat accgaaggtc
     8701 agctgattga acaccgttta aggaagagat tgtgaaaaag tcgcttagtt tcgagtatta
     8761 agaaaaattt tgtgaaaatg gatttctaca tttttgcata aatttggctt aataccgaga
     8821 aactggcttt tgaataccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta
     8881 ttagagaaac aaacggaaaa atagctgctc atattttgcc gttttttgag ctaataccga
     8941 aggtcagctg attgaacacc gtttaccaag aggttgtgag cctaccattt aactccgaat
     9001 cacaagaaat aatttcaaaa acaacttctc ctgtttttgg ttcggttggc ttattatcga
     9061 gaagtagcta ctgggagtct atttatcaaa aaaaggaatg cgacataggt cacacccctt
     9121 ttttattttc tttgaaacat ctttcggtac tcgacagcat acaagacaaa agattgtaaa
     9181 agaccgtaag tgatgacggc aaaaatgact ctcacccaca gcgttggtaa ttctgggaaa
     9241 agcagcaaaa ggatcatgcc aacatagatg acagcagtcg ctactttgcc ataccacttc
     9301 gctccactta atttgatatt tttagatagc ccgattaatc cagctacaaa cataaagcct
     9361 tcttttatca agaatagacc aaacaacagc caaagccacg gtcgatacca tgccatagac
     9421 aaaatcagaa aaagttgtgt caatttatct gcaaacggat caagcacctt ccctaagtct
     9481 gttacctgat tatatctccg agcaatgacc ccgtctagat aatcagacaa acctgagaca
     9541 acaatgaccg ctcctgctat ataaaatgac tgtctcagca caaaataaag ataaatcggc
     9601 agcaaaaaaa tacggaatat cgataaaaga ttcggaatcg tttgtatttc tttacgccag
     9661 tctgtgctta atttcatttt atcgccctac ctcctcagca ctcttttctt tttattttag
     9721 cttacacggg gaataaaacc aatgatttca ctgtttttat ttttctttac agacactgat
     9781 attatgagct aatttgagat aatcacttgc tgttttcatc gtttactctt ctttcttcct
     9841 gtcgtttgcg catttctttt actagtactt tatgagcttt ttttgatcct cgctctaggt
     9901 cacgctgccg tttacgtgtt ttataatcga taaacagact gtctaaatcg taacctgtcg
     9961 ggtacagatc ttctgctgaa cgcaccagac gaacacgctg acgaggcacg tggattattt
    10021 ttttgtctaa atatacatca atcgtatctt cacctatatc ttcataaact aacgcttctt
    10081 tttggtattc attgacaaat atccgatcac cttttgcaaa gagctgacgt tcttttttat
    10141 tctccttcct atttgtaact gctgtcttag gaagaaattc ttttttctgt gtccgatagg
    10201 ctttttccgc caagtaacgc tctgcttgat ggatcaattt catcgacata tccattttat
    10261 gagcaatcca caaagcttgg ctttctcctg tttcttccaa cccgcaactg atatttagga
    10321 cgaagtgctt cccgatcgaa agccatagca gccggcacaa aatcttcatg atctccggcg
    10381 aatttcttga tttctccata atgggtcgtg gcaatgatca atgccccttg ctcatacata
    10441 gattccatga ttgcgatcgc tagtgcagcc ccttcatttg gctctgttcc actgccaatc
    10501 tcatctaaaa gtaccaacgt attccttttt atctttttca aaatagcagc gatgttttgc
    10561 atatgaccag aaaatgtact caacgcattt tctaaatttt ggtggtcacc gacatcgacg
    10621 aaaatctcat caaagacagc aagctctgtc ccttcttttg ccggaacttg cataccgaac
    10681 atcgccatca aagtcaataa accgactgtc ttcaacacga ctgttttgcc tccggcattt
    10741 gctccggtga taatcaagcc tcgatagtct ttaccgattt caacatccaa tggtaccgca
    10801 tgctcaggta aaaatggatg tctgccttgc ttgataatga tatgttccga tttattgatt
    10861 ctaggtgtgc ttccattgat ttctcggcta aattttgccc gagcaaagat gatatctaac
    10921 accgtgatcg tttcgatcaa taaatcgatc acttcttcgt tttctgcgat agcaccagtc
    10981 aatgccgcca ggacttgata ttcttcactg atttcttcag cttttaacaa ctgatagtgt
    11041 tcattcaatt tagaaatcac agttggttca ataaaaaccg tcgttctttt atttgattca
    11101 tcgataatcg ttccatctat tttattttta tagctggctt tgataggaat cgtatagtat
    11161 tcgccttttt gaacaatcat tgcttcttgg atcatttctt tgtttttcgg atgacgcaag
    11221 aatttcagta attttgactg gatttctttt tcgattagtt gtaattgttt gcgtacttta
    11281 cgcaaattac gtgacgcgtc gtcactgact ttattatttt ttattttctg gtcaatcaac
    11341 tcttctacat ttatcaaatc aggaagatgt ttgctataag caaacaacaa gggtgtctgg
    11401 tattgattct tatcaaaaaa ctttgtgatc atccgactgc ttctaagaaa atccgcatat
    11461 tcgatcaaat cggttggctg cagaatcaac ccttttttga cttgttctgt cagtgcatcg
    11521 atcctcggca accccataaa tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct
    11581 cttgtttctt cttgacgatc caagacagtt tgtaggtttg tcgagaccgt catatcgctg
    11641 attcgttttt tgctatagtt tccaattgct cttgcttgga tttccttttt gatccgatca
    11701 aattgtaatt gactaatcat ttcgttgtcc atattctctc cttttcttga agagatacct
    11761 gtcccaaaat aaataagggc ctagagataa ataaaaggat agaacacagc ttaaaaaagt
    11821 catgtcctat ccttattaga atacaaagat attgttttcc tgacaggtac cgtttcataa
    11881 gcacaccaaa taaactttgt ttattggcta ttcgacttac tgattagata ccagctacac
    11941 tcacaaaaca taccccattc ttctaggtct tatttatttt ccacttaact atagcaagac
    12001 acattttggt tgtcaaactt tagtatgttt tatagattta caccattaat gaatagacac
    12061 ctaccgcgat aattgctccg atcattggac ctgcgattgg tacccatgaa tatgcccaat
    12121 ctgaagttcc tttattagct attggcaata ccgcatgtgc taatcgagga ccaaagtccc
    12181 gagcagggtt gattgcatat cctgttggcc ctcctaatga aagaccgatg gctaagatca
    12241 atccacctac agcgaatacg ttcgttccag gtgcaaattc gttttgtcca aaagctagca
    12301 acccaagaac taatacaaat gtaccaatta attctgtgat cacatttgct ggataattcc
    12361 gtatagcagg acctgttgca aacgttccta aaatcgcgct ttcatctttc gttgcattcc
    12421 aatgtggcaa ataagaaagc caaacaagaa tggcccctgc aaaagctcct aaaacttgcg
    12481 caatgatgaa aggaacgaca agattccaac taattgcccc tgtcatcgcc atagctagag
    12541 agactgcagg attcaaatga gctgggccca tgaatccaga aatatagaca gccattgtta
    12601 cagcgagtcc ccaaccaaat gcgatgacaa tccagccaga agcctgtgct ttacttttgt
    12661 ttaaattcac agccgcacac acaccatctc caagcaaaac taggatcagt gtcccaataa
    12721 actcccccat gatttgagtc atatcactgt tcatttattt ctcccccttt agttcccgca
    12781 gatctgattc tgcaatcaat gcttctaaac gtttagtctg ttgcgccttc tcctcttctg
    12841 tccattcaaa atatccagca attgcatcga tcacaggttg cttgatctca tcaagctgat
    12901 ctcgttcaaa caacagatga tttgtacgac gaatgagata atcgcctggg gccaaaacca
    12961 tttcttcttc taaaccataa cgtaaccgag ctgactcagc gagactcaaa ccaggataag
    13021 gtgccatttc ttttgctaat tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga
    13081 tataagtagc atcttcttcc tctaaaccgg ctgctactcc ttcttttgct aattctgtca
    13141 ctgtttcttc taatttagtt ggatcgaagt ttccacctga aatctgatat tttttagagt
    13201 cgatttcttt cgtctctatt ccgtattctt cttttaataa ttggcgtatc aatcgcaaag
    13261 ctccttctgc catcttacgg taatcggtga ttttcccacc agataaagtc agcaaaccat
    13321 ctggttctct ttctaaagaa ctacctctgg aaatcgtaga aggtgctttt tcatcacggc
    13381 tgttttccaa atggttcaac acgtcttcta cttcagcacg agaaactttg ttttccttat
    13441 attcacttac tgtatcgaca actttattga agctcttgtc tgaaatcgat ccattatctc
    13501 caccattgta atcagaacca gaattaccaa tcaacagtgg acgaagtcct gcccagcttg
    13561 cttcgatatc attcaatgtg atatttgctt ctggatagcg atgattgatc acatccaata
    13621 gataatctac atcttcttgt gtgactttcg gatccgtaaa atctccttgg taatccgtat
    13681 ctgtcgtacc aaagtaagtc ttgttttctc ttggaatagc aaaaaccatc cgcccatctt
    13741 gctttcctgt atcgaagtat gtgggttgcg gtacaggcag ttttttcgca tctacaacta
    13801 aatggatccc tttagttgga cgcattttag gagagactgg acgcgtaaaa ttcaagtttc
    13861 gtactttatc tacccaagga ccactcgtat tgatcactaa ttttgctttg atttcgatca
    13921 cttcatctgt cagcaggtca cgagctttga cgccaacgat ttgatcgcca tcatataaaa
    13981 agccaaccgc tttcatttta cttactagat aagccccatc ttcggcagcc tttttgatat
    14041 tatcaatcac taaacgggca tcattgttgc ggaaatctag atagacaccc gcgcctttta
    14101 gcccttcttt tttcaaaaac ggttctcttt ccaatacttc ttctggtgtc agcgtataat
    14161 tctcatattt ggttcctgtc acatttgcca gtttgtcata aaggtccatt gctactttga
    14221 ccgagaacat attgaaggtt gttgctcctt cgccctcata gattggcaaa agcattggat
    14281 ctggttttgg gatgtgtggg gcaatacttt gtacgactgc acgttcacca actgtgtcag
    14341 ctactacttc cacatcaaat gtcttcagat aacgaatacc gccatgaaca agtttggtcg
    14401 agcgagagga agtcccttct gcaaaatcct gcatttcgat caatcctgtt ttgattcctg
    14461 atgctgctga ctggactgcc acaccggcac cagtgattcc gccaccgatg atcaacagat
    14521 ccaattcttc ttgctgcatt ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat
    14581 ggatttcctc cttattcaaa tgcttgggta gcttttactg cttttttcca accgttgtaa
    14641 agtttttctc ggcgctcttc ctccatttga acttcaaata gcttgccagc ttcataaaat
    14701 tcgcggatct cgtctgtatc tttccaataa ccgacagcta atcctgctaa aaatgctgcc
    14761 cctagcgccg ttgtttccaa gttttctgct cgttggatcg gtacattcaa gatatctgtt
    14821 tggaattgca tcagatattc attgtttgct gcaccgccat ctactttcaa tacaggaatc
    14881 ttgattccag tgtcatcttg catcgtatcg atgatatctc tcacttgata agcaatcgac
    14941 tgtaaagtag ctttaatgat atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc
    15001 cctcgtgctg cctgatccca ataaggagca cctagcccaa cgaatgcagg aacaacataa
    15061 acctcatctt cgcttgtaga gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac
    15121 atttgaagtc catctctcag ccattgaatc gaagaaccag ccacaaaaat acttccttct
    15181 aaagcatagt aaacctttcc atttatgccg taaccaattg tcgtcaataa attatttttg
    15241 gataattgcg gtttttcacc ggtattcatg acaataaatg aacctgtacc ataagtattc
    15301 ttgaccatgc ctggttcgaa tgccatttgt ccgaataatg cggcttgctg gtctcctgcc
    15361 atccctgaaa ttggtgcttc gcttccataa aaatgataac cttgcgtata accataaact
    15421 tctgagttag aagtgacttt tggtaacatc actcgaggaa tatttagtaa atctaaaatc
    15481 tcctgatccc aatctagctg atggatattg aacaacatcg tccgactggc attcgagtaa
    15541 tcagtaacat gtgcttgtcc atcggtcagt ttccatacta accaagaatc gatcgttcca
    15601 aacatcaatt cgcctttttc agctcgttct tgcgcgcctt ctacatgatc aagaatccag
    15661 cgtatcttcg ttgctgagaa ataggcatcg atcactaacc ctgttttttc gtggaaaaat
    15721 tcttgatagc cttcattttt taagttatct gcaattccag ctgactgtct ggattgccaa
    15781 acaactgcat gatagattgg acgtcctgta tctttttccc atattactgt tgtttcacgc
    15841 tggttcgtta tgccaatccc agcaatctga tttggtttga tccctgattc gatgaacgct
    15901 ccggcaataa ctgactgtac agaattccag atttcatttg catcatgttc cacccaactt
    15961 tcttgcggga aatattgcgt gaactctttt tgagaactgc cgatgtgtct tcctttcttg
    16021 tcaaagataa ttgctcgtga gctagttgta ccttggtcaa tcgccatgat atacgttgat
    16081 tctgtcattg tgattccctc cattatatgc aaacgctttc tttaactact cttatcatac
    16141 tactgtttta aaaaaagaaa ctgtcaaaat tttattcttt ttttcttttt ttgacagttt
    16201 tttattatga agctattttt taatcaattc ttttcttttt tcagcgtctc tattcttctt
    16261 ttcaatcgaa taatgtcgta aggtgacgca aattctgaca gataatattc acactgtttt
    16321 ttgtttctct ggcaaaatgt gatcaccaaa tcatattctt taccttcttc ataacttcca
    16381 atctcaatac cgccgattcc cctcagttct ctgatcagat attgttgaaa agcaatacga
    16441 taaatcggca gactttctaa atcgattcca atcactactg gttttgcaat ataaaaatcg
    16501 atcatcaaaa gaacattggc atactggatc atcaggtaac taagctcact atcttcagct
    16561 cttttcctcg gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag
    16621 gaatgcccga gcattttctg ctgctgttcc aaaagccgat cacgctcata gacttcgatc
    16681 tttccaacaa agaaaaacca ttcattgttg atctgggaaa tttggtagcc tactgccttt
    16741 tcttctttga ttttcaaacg acgaggacga taatgccata acatcgtttc ccgaatgtaa
    16801 gtatctaaaa cagcggttgg taactttttg ctccgtgtca aatcatactg ataaaatatt
    16861 tcttcgtcta ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt ttcgtataca
    16921 ttcagttctg ctgctgttcg actaagatgc atcacgatga ttggatcgat tgcttgataa
    16981 agccggtccg actgatagag taatttcttt tctttcaaag ttgcgtacgt acttttttca
    17041 ttcaatagtc tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc
    17101 gtaaacgttg tttctaatac acggtccaat cctagaatga acggtctttt ttctggtgta
    17161 ttttgcaaaa aaagcggtcg ctgatcttcc gggatatatt ggaatagttc ataataaaaa
    17221 tatcgtatct gtagctcttc tccgtataat tgaccgtttt tgatttgtaa tccaaattcc
    17281 tctaagagct ggttcaattc tttgatcttt cgaaaaacag aagactcact gatattaaac
    17341 gctgtcgcta atcgaacgat ggatacttgc tgatgttcta ataaatagac gagcatctta
    17401 aacttcaagc tttcctgcaa taaaaacaat agaatttttt ccaaacttac atcttcgtcc
    17461 aacacaagca gcgcctgata ctcatttcgc tgtacttcta cttttttttc catcattcgg
    17521 ccaatcaatt caatatcttc caaatattga tcaaacgagg cttttgacag ttggacatgc
    17581 tctctcaaac cattgataga ggctgttccc ccttgcagaa caaggtttcg caaaatcagg
    17641 atctgtaact gctcttttct ttctaataat gcttcaatct tcataggcag tattcgccac
    17701 ctttgaccac accatatctg gcacacggta ttctggctgg aaaaatagct catgttcttc
    17761 taactgttca aacgtccatc cttcttctac tgcaattgtc agttggtcaa gaaggtggaa
    17821 catctctttt ttgctttgga gctgtgctcc aagaatcttt ttttcagaat caaatagaaa
    17881 tttgagttta actttgcttt gatttattgc tgtaagcttg tagtctacgt atcctgtttc
    17941 aattttttgg ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata
    18001 gccaaaccac tgattgccga ctggacgata ggttccctga tcctttgtct gtgagccact
    18061 aattgtcttg ctgacatttc gtgctgtacg gacagcgtta tttactagag aaacatacat
    18121 tttttcattt gttggacgaa aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt
    18181 ttgagacgtt tgcaagtagt catctaccca gatcgttcca tcatcattca atgttaattg
    18241 ttcttcccac atctgattgt cgggacgaga atgattagcc agcaaaacaa gatcatagtt
    18301 ctcctttctt ttttccgtct ctacaatagc ggtttcattt tcggtcaaag acacaacttg
    18361 ttcttctaaa aaaagtgaaa gcccttgatt tttcatttcc tttactaacg gttctgtcat
    18421 ttcaggatct agataacgaa aaagaattga ctttcggctt tcgtaaagat ggatttgttt
    18481 ttttcttgat gccaaccctt cagctatttc gattcctgct tgtcctgcac caataattgc
    18541 aattttcttt gcattcatga tcttttgctg aagagccgtt acgttttgta tctttttaac
    18601 tgggtgaata agcgttgatt ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc
    18661 tagaatcaaa cgatcaaaat ccaatttttt tcctcctgct aaaacgactt ttttttcttt
    18721 caatcctatt actgtctttt ctgtgtaaac atggattcga tagcggtcag tcagttcttc
    18781 tttcgtgatc caagaaagtt gtttttcact ggtaaccttt ccttttaata tcaaattgaa
    18841 cccgctggga atccatccta acttcgattg cttttcaatc aaatatattt cacttgttgg
    18901 catcaattgg cgaagataaa tagccgcatg aatccctgca aacgacccgc cgacaattac
    18961 aatgttcatc tgcattcctc tttcttataa ggtgttctac tgctaacttc tcaatcagaa
    19021 aacacagtat tcataactcg ctttcaacct tatgatacat agcgatgcag cagaatcaac
    19081 cctctccatc ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg gacctaattg
    19141 aagagtatca tttctgaaga gatgtttcaa aaaatgtact cttataaaag aaaaaaactc
    19201 gccttttacg tacctctgtc caaataggaa acagaaaaga acgaaaagac gagttttagc
    19261 gtgtgtaatg taatagttag aatagacaca tcatttattt ttaattcttt aatgcttcaa
    19321 caactgcttc gcctaattga tgagcagatt gaggattttg ccctgtaaga agccgcccat
    19381 caatttgaac cacttctgaa aaagctgctc cagactgata gtttgctcct ttttctttca
    19441 aagcatcttc tgctaagaat ggtacttcat tcgttgtacc atttgcttcc tcttcttcat
    19501 tactgaaccc agtcactgta cgtccagcta aaatagattt tccagactca tctgtaactg
    19561 gcaacaatcc tacaacaccg tgacaaacag ctgagaccag cccttcattt cgatagattt
    19621 gtgaagcgac tttcccaaga tctattgctt ctggaaaatc ccacattgtt ccgtggccac
    19681 cagcataata aaccgctgca taatcatttg catccacatc atcaggacgt aaggtatttc
    19741 ccaaggcccg atttctgaaa tcttcatctt cataataggc ccaatcgatt tcatccatat
    19801 tcaaaacact ttgcggttct aaaggtacat atccgccttt aggactgaca aaatctgcat
    19861 cgatcccatt ttttactaat acatcataaa aatgagtcag ttcacttaac cacaaacctg
    19921 taggacgctc gatcttttcg tacttttcta catttgttaa tacgatcaat actttcattt
    19981 tcccatcctc tttctataaa aattgcgtta ttgataacgt gctttcaaga aattatataa
    20041 tgggcttggc aaataaagct ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc
    20101 ctcacggttt tccaataatt tctctgccca gtcaggatcg acgatcattt gctgtccaat
    20161 tgctgcaagt tctgcatatt tcaatacctg ctctacatct tcgcgattgc ggatcccacc
    20221 gacaccaact aaagggatct ttccttgaat cgtgttatgg accacttcaa gaatcgattt
    20281 atcgttgtat ttatcagaac gagctattcg atcatacata tttaatgaaa catgcagata
    20341 atctagtttt gactcacgta atcgttcaag aagccagatc gtgtcttcaa aacggatacc
    20401 tggtgtttca tattcttctg gtgagaaacg ataccctacg atgaatggtt ttttagcata
    20461 ttgttcaacc gctgagaaaa ccgttgttaa taattcttca ataaaacgat aacgattttc
    20521 caagctaccg ccccattgat cctctcgtct attcgaatgc ggagagaaaa attgttggat
    20581 caaatacgta tttgctccat gcaactcgat tccatcaaat cctgattcaa tcgctctacg
    20641 tgtggctttg ccaaatgctt ggattgtttc aacgatttct tcttcactca ttgctcgtgg
    20701 tgtttccgca tcaggacgtt ttgctgcaac tgcacttgca gatacaggct gttctccaga
    20761 taaagttgct cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc
    20821 ttctccttgg attgctttcg ctagctcact taatcgagga atcatttcat catgagcgat
    20881 gctgagttca cctggccagc cttttccgat atcttggaca tttgcagcac cagtaataac
    20941 agcaccaaga cctttagaac gttgtttata ataagtgatc tcatcttcgg tgatcactcc
    21001 attgaaaaag ctgagttgtg tcgtcatagg cgacagcatc aatcggttct tcaattgaac
    21061 tcctgaaggt aaagtaattg attgatcaaa ttgtgacatt gtcattcctc ccataattcg
    21121 ctctgattaa cctatcgaga atcatctatc attaaaccac tcgattagtt gtacatgcaa
    21181 ctaatttgct taaaaaattt tatttttttt gtaacttatc tgttgcatca ttcattgtct
    21241 gggctaattc tttacttgct tcatttccca aaactgcttt gtatgactga tgaagttctt
    21301 cccagctacc atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg
    21361 acaagcgacc ttcgctaact gttttgacgt atcctttata ttctaatttt tcaacaaaac
    21421 gagcaatcgt tgatggggcg gaatagagca tttgtgcaat atctttttgt ggtattccat
    21481 ctttttcttt taatatcaat aaaataaaac cataagttgg tgccagacct gtccttctaa
    21541 acgcttcatc agctaattta ttcaacatgc gatctagctt tttcacgcta aagaaaagac
    21601 attcatctaa cgctttttca attggctttt ccatttcatc ccctccattg gcgtacatat
    21661 catagtatga tttttatttt ttgtcaaaaa acacggaata atgtccgcct cctctgtcta
    21721 gatttgaaaa aaagaagtag ccatcttcaa agaatttcgg cttgttttcg acaaaactcc
    21781 ttctggaaca ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa
    21841 aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac cgaaaaactg
    21901 gcttttgaac accgtttata tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag
    21961 aacagccaaa caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc
    22021 agctgattgt acaccgttta ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa
    22081 ggagcaattt caaaaaacag cttctcatgt tttatgacta tggtgacttg tgatcgagga
    22141 gttggcagga gaatactgtt tattcgtaaa gaaggggctg tgacaagact tttgtcacag
    22201 tcccttattt ccttttatta taggatttcg ccatttgatt ggatgacttg tctgtaccaa
    22261 tcaaatgatt ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca
    22321 tagatatatc cataacgttt tgacatttgt gaagtagaag cgctgacgat atcaatgcag
    22381 ccccacgacg tataaccaaa taaatctact ccatctttga tagcttcctt catctgctca
    22441 atatgctgct tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc ttcttctact
    22501 ttgtctatag ctccgagtcc gttttcagct atgaacagtg gtttttgata cctatcatat
    22561 aattgattca aagcaattct caaaccctta ggatcaatct gccagcccca ttcacttgat
    22621 ggaagatacg gatttttaat tcctccagtt attaagtttc cttggacccc ttcccaattt
    22681 tctggatgag cactgactgc actagacatg tagtagctaa aagaaacaaa atcaacaggg
    22741 taggctgcta agatttcttc atcacctact tctattttta agttgacacc tttttcttca
    22801 aacattcgtt gcgtatacgc tggataatat cctcttgctt gaacatcact aaagaacaaa
    22861 gaagttcttt gctgctcaat tgttttttga atatcatccg gatcacaaga atacggaaca
    22921 agaagctgat agctgatcat acaaccgact tgtgaacctt tgataatttc atgacaagct
    22981 tttgtagcta aagcacttgc tataaactga tggtgtgcac cttggtattt cgcttgttcc
    23041 aaatggggat ggttttcttc aattactcct acgctaacaa aagggtgatg ttttacacag
    23101 ttgatctcat tgaaagtcat ccaatatttt actttatttt tatagcgttt gaatactgtt
    23161 tcagcgaaac gaacgtaaaa ctgaataagc tttcgattag gccatccacc atattctgta
    23221 accaaatata acggcatttc ataatgggaa agagaaacga ctggctcaat cccatatttt
    23281 agacattcgt caaacacttg atcataaaaa aataatcccg cttcattggg tgtttcttca
    23341 tcaccatgag gaaaaattct tgtccatgcg atagacatcc gatagcattt aaaacccatt
    23401 tcagcaaata aacggatgtc ttctttaaag tgatgataga aatcatttcc gtgtctttta
    23461 ggataataat acttgctgtt gggatcctgc gccgctttta cttgctgatg cgtcatcata
    23521 tgccattgat ctgtccattt ttctttaggt aaatcgctgt cgaatgtata cacatcagac
    23581 acagataatc ctttttcatc tacatcccat gcaccttcac attgattggc cgctgtcgct
    23641 ccgccccata aaaatccttc tggaaatttt ttcatatcga tccccctaaa cttgctagtt
    23701 ttcttgtttg attatttttc ttatgactag taatgatatt tttgaatcta ctactatttg
    23761 taacaataac cattgtggtc ggatcatatc cctctttgat taattcttgc aaatctactt
    23821 ccatcaataa atcgccttta cgtacttttt ttccatcaga tactgcactt tgaaagtatt
    23881 tgccatttaa attcactgta tcaataccga tatggatcag tatctctgct ccttcttcac
    23941 tgactacccc aattgcatgt ttcgttggaa aaactgttga aacttccccg tttacagaac
    24001 tgtagatttt tccgtccgtt ggaatgatcg caaacccttc tcctaaagat ccactagcaa
    24061 atgttggatc gtttacttca gataaatcaa ttatttcacc tgctgcagga gtacttaaca
    24121 ctatatcctt tgaggattct tcttcgattt catccttgta gccgaaaaga aaagttagca
    24181 tacaggaact gatcatcgct aagaaataac tagcaactag ccaaacaaat ccttcaccaa
    24241 tataaatcgg gaaagacaaa atacttcttg tagcaactgc caaagcaccg gctcctgcgg
    24301 caccagctac tccaccaaat atagctccac atatacaagc aatgtaaaat ggtcgtttat
    24361 atttaagagt cacaccataa acagcaggtt cagtgattcc aaataatgct gtgataaatg
    24421 cagataaaga taaacttttt actttcttat ttttcgtttt caaaaatacc ccaagtactg
    24481 ctcctgcttg cgcagctacg gcagtcatac atgctggacc tagcgtatca cgaccgtaga
    24541 aagaaagatt gttggtcatt actggtacaa gtccccaatg taacccaaaa atcactaaca
    24601 cttgccaaaa tccgccgata acagcaccag ataaaacagg gctaaagtta taaacagcta
    24661 aatatccagc tgcaatcagc tgacttgcat aatctgcaat tggacctaca actaataaaa
    24721 taattgggac catcacgatg atacaaatga atggtgtcaa taaatttcga atactttcgt
    24781 gcaagctttt gcgaatctta ggctcaatat aagaaagaac ccatacagct aaaattgctg
    24841 gaataaccgt acttgtatac cttgctagaa tcacgggcat ccctagaaat cgcaaggtta
    24901 tgctatcact atatgcactg acaatcgaag gataaacgag agctgctgca ataaccatag
    24961 cgatgaaacg atctgtattg aacttttttg cagcagtata tgccaaaaat ataggcaaga
    25021 aatagaaaaa tgcatcagct gctgcattca aaatgatata agttcctgat tcagtagtta
    25081 ataaattcaa actcgtacat aaggcgagta ctcctttgat caaaccactt cctgccaaga
    25141 cgcctaaagt cggggcaaaa acagcggcga tcatgtctat aaaagagttt aaaatgcctt
    25201 ttttctcagt tcctctcttt ccactagatg catcatcttc tagtttcatt tgtttggata
    25261 atacgtcata cacttcattt actgcatttc ctactacgac ttgaaacatt cctccagctt
    25321 cgactacagt gatcacacct tctagttgct ctaccttctc cttatctgcc ttcttattat
    25381 cttttagggt aaagcgtaac cttgttgcac aatggtaaac attgctgaca ttttcttcgc
    25441 ctccgacaaa tcggacaata tctttcgcta attgtgaata gtccattttt acttccccct
    25501 tgttaattaa tgccgtagca atccttctta caagataagt atagttctta gaaaacggtt
    25561 tctccattca attagtaccc cttctaatag cgaacgaaac tgtacaatag tgtgtatggt
    25621 ttacttagcc gagcaaatca ctcactgctt gaaagaaaac aagtatatcg tttctcttat
    25681 agtcaaagga gacaaaaaaa acacgcttga tcttcctttg ctgatgtata agtggtatct
    25741 caagctgata ctctgtgatt tttgtttcta cttggctttt gataagacag cacaacaaag
    25801 tctcggcatt taacggaagc ataactaggt cagaactagg agagattagt tgctgaatgg
    25861 aatctatctt tttatggatt tttatttgat tttcttcttt gtagtaatga tcaagcagtt
    25921 ttgttttctt tttttcttca actatggtca gcttttcacg caaaatctga taaatctccc
    25981 aagattcaaa ctcatctaga acactaggta gataaatcaa atcatcccac tcaaaaataa
    26041 gctcttgctc agtagtcaaa attgcatcat attccgttat tttgtttgat tgatttttga
    26101 aaagatacac tggttctatc cggcattctt tgatccacat gttcatactt tgttgaatct
    26161 gtttttttag tgtattcaaa atactaattg gctggttact aacaatcagt atctcccctt
    26221 gttttcgaaa aggattcaac gcttcggcaa gatacaaaac aaaataagcg ctttcatcta
    26281 aattgggacg taattgaaaa tgtattggga agaaacgccg aattaaataa atttctaaag
    26341 gataacggga gatgatcttt tgtacaaaat gattcatggt attatgtccg gcatccatcc
    26401 tcgttttcat ttgtttgata tgggttagca aaaattctgt ttcatggaac aataacgaaa
    26461 caggaagatc taaaaaacga ataatcgttt gctcaaatgc tcttagattc ttctctattt
    26521 ctttgttttt ttcatcatta atcatcaaat aatttaactc taatagtcga ttttcaattg
    26581 cttgtttttc ttgattggaa aaagaatagg gtagttctga tgataacttt tttgtgaggg
    26641 tctctaccat cggaataaat aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc
    26701 gaagaatggt caaaacgaga taccgagaaa atcgtatgaa gtcctctcct gaaacaatat
    26761 aattatatga tataagaacc tcttttaatg tcttttggac agtcggcatg atattggaaa
    26821 aaattgtact gatttgttga ggcaaccttg cgtgttggat caccgtttct gttccaacaa
    26881 gagataagaa aattcttttt gaattttctt cagcaatgat ttttagtcct tttgtcgacg
    26941 aaacttccag ttctaattta ggattgcgct cgatccaacg caaaatcgtt tttatttgta
    27001 atgatacggc tgtttttgaa agaaagaaaa tgtctgctat cttttgcata ctgacatacc
    27061 ctttttgaaa caataagtac gtcacaatag ttgaagctcg gaaatccatg ctggaataga
    27121 ttttatgttt cgtcacctct tgtatgattt gctgtttagt ttcagattca attgattcta
    27181 aaaaataacc tgaacgttta tttgactgga taatgatttt cttctcgtag atagcaagca
    27241 tatttttgat ttcagtttgg atcgttttct tgttggtacc taaagcttca gctaattctt
    27301 cgcttgttat ccaatgattc ttatttctta atactacaag aatttggagt tgtcgtttat
    27361 ttaagtccat ttgcttacac ctcctctttg ttataaacat acaggaaaaa cctctcaaat
    27421 gacaatagtc acttgcagag gtttaaaaat catttgaaga aagatctata ttcaaggttt
    27481 ttaccattag actggataat ttgtttataa tattcaaaac tatctttttt caatctccta
    27541 aagcttccgc tcccatcatt ttgtctatcc acataaataa agccatatcg cttcctcatt
    27601 tcccctgttc ctgccgatac gatatcgatt ggtccccacc ataagtaacc aatgatttgg
    27661 caaccatctt ttattgcctc agcaatttcc tgaagatgta tttttagata ctcacaacgt
    27721 tttggatcgt ggattcgatt caattcatct ggtgcttcat ctagtccaat gccattttca
    27781 actatgaata atggtaattc ataacggtct gtcaattcat tcatgacata acgaaaccct
    27841 agaggatcaa ttggccaact ccaaggttcg ggagattttt ccttcaaaaa gggattatcg
    27901 aaaccatttg ctccgccagt atctacgcgc atttctgctt cttttttata gacagcgcta
    27961 cgataataac taaatgcaat ataatctgcc gtgttagctt gcaagcactg ttcgtctcca
    28021 tctaacatta ctggtgcaca gtcttcttcc ttccatgtcc tgtaaatata agctggataa
    28081 tgccctcggc acatgacatc ggagaaaaac catgaattgc gtttaaattg cagtgaacca
    28141 aatacattct ctggatcaca gtcatatgca taagtcgcta atgcagaaaa gctacacata
    28201 atacccatct tagcatttgg atcgattttc tttaataatt gagtagcacg ggcgttcgct
    28261 agaaacatat gatgacaggc ttgataaatc actttttttg ttagtgtagc aatgggttca
    28321 agtttatctc tagcagggaa tgtaaccaat ccagcagctg cataaggcat acgaaaagca
    28381 ttattgatct cattaaaggt catccaatat ttcactttcc cttggtagcg ttcaaagacg
    28441 gtctgcgcat aaaattcaaa aaagtcgatt aattttcgat tttgccaccc cccatactct
    28501 cctaccaatg ctaagggggt ttcataatga ctaagagtaa ttactggctc gatatcatat
    28561 cgatttaatg tattgattag tctgtcataa aatacgagac cagcctcatt tggcttctgt
    28621 tcgtctcctc ttggaaatat tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct
    28681 aactccttca gtaaacgaat atcctcttca aatcgatgat aaaaatcaat cgcttcatga
    28741 gaaagataat ggagtgattc gtctaaagca atctcccaaa tctttttttc ttcattccac
    28801 tgaatactag gatgctggtt catgatcccg ataagcgtat ctgtcacatt aggtaatttg
    28861 ccgtcttcca accatgcacc ttcacattga ttagcagcta ctgctccgcc ccataaaaaa
    28921 ttttctggaa atatttcctg atccataact atctctcctc gctatcaaca atttcttact
    28981 tgaagtatag ctcgattcat caaaacaaga tattctaact atttcggcag aagagaagaa
    29041 aaaaattgaa tgctactctt catttttaat atttatatat aacaaataca taagacgatc
    29101 cccgaaagaa aacactcgtt aaccatctaa gcaaacgatt tattttactt ccaacacaaa
    29161 tgcttcataa ggtcgtaaat caactttgcc gtctggtaac tgtttcagag gataatttga
    29221 cacaataacc cttaaagctt cacgtggaat agtgaacgtc tgcttttcac tggacatatt
    29281 acaaataacg agccattgct ggtcttcata tatgcgagaa taggcaaaga catgctcagg
    29341 cgtcttttcc actaattcat agtctcccca gacgatgatg tcatgttttc tacgcaaatc
    29401 gatcaatttc ttgtacatat aaaaaacaga atcttgatct gctaacgctt cttctgcatt
    29461 gatttcttta taatttggat tgactcgaag ccatggaatt ccagtagtaa acccagcatg
    29521 ttcacgggcg ttccattgca taggcgtcct agcattgtct cttcctttcg ccttgattga
    29581 ttccaagatt tcctcttttg taaatccaga agaaatcctt tcgttataca tattgattgt
    29641 ctcaatatct tttgcttccg cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc
    29701 tccttgatag atataagggg tgcctttcat catatggagc aagatagcaa acatcttggc
    29761 actttctttt cgatacacat cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg
    29821 attattccaa aataaactat tccatccttc atcacctaaa gatgtttgcc atttggataa
    29881 tacttttttc agttcagaga tactcaaggg tttcaaatcc catttgtctt tgccttcttg
    29941 ctgatctaat cctatgtgtt cgaattggaa gaccatggat aattcatttc ttttaggatt
    30001 ggaataaagt ttagcgattt cggtagttgc tccccatgtt tcgccaacag tcatcagatt
    30061 tttgtcacca aaagttgctt gattcatttc ctgaagatac tcatgcagtt taggtccatt
    30121 acttgtgatt tctttatccg gttctttgcc aatcatatcg atcacgtcca tacgaaagcc
    30181 cccaatacct ttatcgatcc agaaattcat catttgataa atctcttggc gaacctgttt
    30241 atttttccaa ttcaaatcag gttgacgctt gctatacaag tgcagaaaat attgccccgt
    30301 tttttcatcc aactgccaag cagaaccact gaatattgag cgcaatctat taggctcttt
    30361 tccattgacc ggatcacgcc aaatataata gtcccgataa ggattttctt tatttttacg
    30421 ggcctcaatg aaccacggat gttcatcaga agtatgattc accaccagat ccatgatgat
    30481 acggatattt cttttctttg cttcttcaat cagttcttcc atttcttcca tcgtaccgaa
    30541 ttctgccatg attgcttgat aattactgat atcataccca ttgtcatcat tcggagactc
    30601 gtatactgga gaaagccaaa ttgcatcaat acctaaatac gccagataat ccaacctgga
    30661 aataatcccc cgcaaatctc caataccatc ttgatttgaa tcttgaaaac ttcttggata
    30721 gatttgataa acaactgaat ggtgccacca tttttgttcc ataattttcc tcctataaat
    30781 ggatgtttct attttttgct taatcattta ttttagtctc tctcactaaa aattgtaacg
    30841 ctttcaaata gctattgcac cttgttatcg gtaacagaat aaaacaaaaa aacagctgag
    30901 gaaattccct caactgtttt ttgaaaggat tgatttatgc agggaagaat gctgatttca
    30961 caacttcttt ataaacggtg ttcactcagc tgtcccttgg tattagctca aaaaacggca
    31021 aaatataaga aactattttt tcgttttttt ctctaatact caggccagaa cgctgatttc
    31081 acaacctctt tatttcacat tcaatatcca catgaatccc acaaatacaa agaacagacc
    31141 atacatgatt ggagatactt cttttcctct tttagctgct aacatggtaa ttggatagaa
    31201 gataaaacct aatgcaatcc catctgaaat actataagtc aaaggcatgc cgatcaagat
    31261 caagaatgaa gggatcgcaa tctctaagtt tccccattcg atttgtcgca aagattgtgc
    31321 catcaatact cctaccataa tcaatgctgg tgcagttact tgtgaagtaa caactgatag
    31381 taatggagaa aagaacaagc ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc
    31441 tgaacgtcct ccaacggcga taccagctga tgattctaca aatgcaccaa caggagaagt
    31501 accaaataag gaacccgcaa gcatagctgt tgaatcagaa gctaaagctt tccctacacg
    31561 aggcatcttg ttgtctttca tgaaacctgc ttggtttgct aatccaacta aagttcctgc
    31621 tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg acccacattt gaagagaatt
    31681 gatgtcccct acatgtttta atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc
    31741 aacaatttca gaaggcattg gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc
    31801 catcccgatg aagatgccac ctggtacacg ccgaacaagc aagatagcag ttacgactaa
    31861 tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat cctaatccta ctaatgtaga
    31921 ttcattcgaa acgatgatcc caccttcgct cagaccaagg aaagcgataa atagaccgat
    31981 accgccagaa atcgcatatt tcaaatctgc tgggatcgca tcaatgatca tttcccgcag
    32041 cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt
    32101 ttcccatgga atccccattc ctaaacaaac agaataagca aaaaaagcat tgatcccaag
    32161 agcaggtgcc gttccaattg gataacgggc taataccccc attaaaacac agccgatcgc
    32221 actggctaat gcagtcgcag taaacactgc tccttcatcc attccagaag cacctaatac
    32281 agttggattt acaaataaga tataagccat tgagataaat gttgtaaatc ctgccagtac
    32341 ctcacgtttt accgtggtgt tcaatttttc tatttcgaag taggaaagta ttttttcctt
    32401 catcttccat ccccctaaat ccgaacatta taaataaagt ccgttaaaat ttcgatttat
    32461 taataaaaca acgtgtttta tcatatacta aaacaaaaca taagtaaaca aaaaattcgg
    32521 attttaagca aaatccgaac gttccatctg attaaatccc tttatctcaa agttcatgaa
    32581 cattcacttt ggattacaag aatttcttta ttgtgttaat aaagtttctt cattctagtc
    32641 tcaaaaatca atagtgtgat ttgattttac catttcgttt ctgttcttct ttgatgaatt
    32701 cttttgtatc gacaggattc agattattta gtccgccaaa acctgtatcg accatttttc
    32761 cccagccttt tgtcttcttt tttcctgatt tgagattctt tgctcgaagc ttctcactat
    32821 gttttgctcg ccgatacttc aaataacctt ctaatcttcc cattagatcc actgtttttc
    32881 ctccttttta ttcaatcaga cgactcagct tttcctataa caaaataatt gccttcaggg
    32941 tcagaaaaat tgaaagtcaa gatgccattg ttatctgtga tttctcctgg ttcttccatg
    33001 ttctcgtaga tagtttcaaa ttcatccgta aaaaatacta aagaaggcgg tggtcctaat
    33061 acctctggtg aaaacttctg cacaaatact tttgggaata cgcctaattc taattctttt
    33121 gagatgctga gtacaatact cttaaattct tctggcaatt cgattgtttc agaaatttct
    33181 gcaccaattt tttctacaaa gaatcggcaa atcatctcta cgtcattgac ataaagcatc
    33241 acacgtgttt tttgtatcat catttgaccc ttctttctat ttgattttct ttcctgtcta
    33301 taaactgatt ccattatatc acgaaagcgc aaacaccttg tgtttgatac tattcaagaa
    33361 aaataaagca atagtttcta ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa
    33421 acggcaatga atgccgctct tcttatttcg attcttttat aattctgctt ctgattcatc
    33481 tttggttagt gtgtgtctaa cactagttac gccaaatgtt tgatcgattt tttccttcaa
    33541 cgcttcgatt gcctgtgcgc cacgtgccgc ttgttcagca ttgatggatt caatgaccag
    33601 caccgctgct tgtgtttctt ccgtcagcat cgttctttgg gcttcacgcc agttgagaca
    33661 gcgacagatc gctccttgtt catcatagta gatgatttct tccggaagtg ccggagcatc
    33721 ggaatctgca cctaaaggca ggaatgattc gccaccttgg gcttttccta aatgcagatt
    33781 tcctgcgatc ttttcgatat cttctccgcc gcaaggtacg ccaaaagaca aggaaatact
    33841 attatagata tctacaagcg gattgattgg gaaaaattcc cgttcttgat ttgcacgttt
    33901 cagtaaagct tcaatagaag aacgtgcacc ttttttagtc ttgaatgtgg taaatgcctg
    33961 acgccactga gcaatgactt cgttttggct gaatgtttct tctgtaaggt aatttcttgc
    34021 ttcttttgct gcctcattca ataattgatg atacgtttct tcactttctt ttgttacgtg
    34081 gttatcgata ccatttacgg ataagatttc gatggttgct tcaggaaata cttcccagaa
    34141 attggtatcc actattactt ttttcatctg ttcacacctc ttgattagaa taattgcttg
    34201 atgaatagat agtctgtttg gggatcatct cccatataaa aaacatgttc gctgtatttg
    34261 ataaatcctt ttttacgata aaaagcgagc gcaacatcgt tgtgttccca aacaccgagc
    34321 caaatggctg ttttttgctt ttcttttgct ttttcaatag ctgtatctag taaaaatgta
    34381 ccgatacctt gatgtttaaa tttttttcgt acatagattc gctcgatttc taaaaaatga
    34441 tcagccattt cctcagattg actcgttcct gtatttagtt tcaggtaacc cgcaggctct
    34501 tgatcgatac tcactaggaa aaaatcggtt tctttatctt ttagctcttc tgtaagtttt
    34561 tctagtgcat aagcttgtgt caagtaatca gctaaatgct ccggtgaatt atcttttcca
    34621 aatgtatctg taaatgtttc gatactaatt tgttgtagta agtctacatc tgaaggcgtc
    34681 atttttcgaa tttcgtattt attcacggct tcttcctttc aatagatcct tttattgcct
    34741 tttttgacaa aattccaatc ttcgctaaca ttcttgcata ctttatttaa aagatattcc
    34801 aattgttttg cttcttcgtc agaaagacca tttaatgcga ctgcattgga gtagttgttt
    34861 tctcgaatga tgaaaggata gatttctgct ccttttttag tcggatagag tttcttgatt
    34921 tttttatttt ctttatcttc caaacgttcg atcatcccat tggattctaa tttcttgatt
    34981 gctcgggcag tcgttgtacg atctactttg atcatttctg ctagtttttc ttggatgatt
    35041 ccaggatttt cgcaaatacg tactaaataa agatactgtc ctcttgttaa atcgacttct
    35101 ttgaattcga tattgctaat agaatctagc gctcgggcaa tcattccgat gtctcgaagg
    35161 atttcagcca tttcatccca cctttatttt gtttgtaaga ctagttcacc tctttttgtt
    35221 gcaaatgcaa ctattttcaa taaaaaaatc aagttctttt ttaataacta gtaaagtata
    35281 ttcttttctt cttttacgaa aaagctcaat cacaatagat ctggtttttg ttttctaaaa
    35341 taagtaactg tttcttcgtt gttcgctttt ctgatagctt tggcaactct tctatactaa
    35401 aaaatcccat gttggaagtt tcattattct caataaactt ggcctcttca ccgtggatcg
    35461 cacaagcaaa aacaagttta taatactgaa aaagctgtgg aatatctttt tgtttattcg
    35521 tatcaaaaac tgctcgcaac tgagtagttt ctactgttaa tcctgtttct tcatagactt
    35581 ctttacgcac attttcttca ggagacaatc ctatttcagc aaatccacca ggaagtgacc
    35641 attctttcgt ccgtttatct tcgattagta ggaccttgcc atcttttttg atataggcac
    35701 gaacatctac ttttggtgtt ggatgtcctt ctccaatcgt tgtgagcgct tttatttctt
    35761 cgaaagtttc ttttcctatt tcactgatca gttccaatga aatcgtccgg atctcctcaa
    35821 aacgctcttt atcaaataca tcttttccat aatatagacc agcttgagaa agtgcgagta
    35881 gtcttttgta ttgagccaac ttcttgttca ttctcttccc ttctttttta gcaaatgtat
    35941 gactatgtaa tcaagtatag aagactggtt taattacggc aaataaatat aatgaaatct
    36001 gcaataaata aaagaatgtt aaaaaactcg agaacaaaag acattcgact tctctggggc
    36061 tgtgaataaa tggcgttcaa aagcttgcat ctgcggtaat aagccccacc aaacgaaaaa
    36121 tatgacgagc tattcttgtc tggctgttct tattaattga ggcaggacag ctttttcaca
    36181 acctccccac taaacggtgt tctcctacca actcctcggt cacaagtcac aatattcatc
    36241 aaacatgaga agctgttttt ttgaaattgc tccttgtgac tcggagctga acgacaggct
    36301 cacgacctct cggtaaacag cgttcaaaag ccaatttttc gatattaagc catttctttg
    36361 ttacttattg tcattgattt ctttttctaa tttatccaat agtggagtgt aatccacttg
    36421 agtatagtct gttacatcaa attcatatag tgttcctaac tggaatttgt catattttcg
    36481 gtcggcgatg atctttttga atgcttcttt cgtaatatga ttggtccctt tcgttcgatc
    36541 ttccaatacg tcaccatagt gataatgatc catgatatag ctcaaatgac gatctgattc
    36601 acgatcacgt ttgtaccgac gttgccataa cacttcaaag tctgctgtta ggcgaatcgt
    36661 gatcacctga tatccgtatc tatctgctaa ttcttggagt cgtgcttttt gcttatcact
    36721 aaaaggatac tcggacacaa tcactttctt tcctgcttcc atatacaaat caagtgcttt
    36781 gtaatagaaa ggataaactt tcgtcctttc ttgttccgcc cgttcttcca aattgttaaa
    36841 cccgatggat tctgcatagt attccttgat ttcatcgagc gtgatggcat acatatctgg
    36901 aaatcgttcc cgtagtttat tcataagata tgtttttcct gtaccaggac taccagccag
    36961 aagaatgaaa tactttttca tctcatttat ttcctaagtt aattggactt tccaagttca
    37021 taaacagtcc aatcttatgc ttgacaattt cttttactgc ttcatttgct ggaatcacat
    37081 tatggcgaag agacttgttg atttctgttt cactaaaggt ttctttagct actttcgtcc
    37141 agttgactaa aagctccgtt ccaacattga atttgcggat cccgttattg atcaattcag
    37201 gataatcttc ttctttcaca cctgttcctc catggataac tagggggaca tcaactactg
    37261 catcgatttc tttcagcaag ggaatgttta cttctgtttt tgatttgaat tgtccatgat
    37321 tcgtaccgat agcaatagct aatgcatcca cgcctgtagc ttctacaaat ttcactgcgt
    37381 cttccggtct tgtatatacc ttgtcatttt cagcgacatg gattccttct tctgtaccgc
    37441 cgattgttcc aagttctcct tctacggaaa caccgtgcgc atgggcatat tctactactg
    37501 cttttgtctt caggatattt tctttgaaag gcagatggga cccatcatac atgacagaag
    37561 tatatccgct gtcgattgct tctttgatat catcaaagtc tcgggcgtgg tctaagtgca
    37621 agactgcatc aaccatttct tcttctgcaa tagctgtcat tacattgact aacacatgca
    37681 tgccgatata tttagcggta tcgacacttg tttgaataat gatcggcgaa cccatttctt
    37741 ttgctgcgcc tagcatagca ggaagcattt ctaggttatg cgtattaaaa gctccaactg
    37801 taaaattcaa ctcttctgct gtttttgtta cttctttcaa tgttgtatac atcttttatt
    37861 gccaccttat ctttttattt gtttcctttg atcgttactt gttttgcaat ttgtcgcatc
    37921 tcatccattt tgcccatgta gtaatcgatc ccttgatcat caccagcacg agcagcatct
    37981 gcacgcttct ggttatagag gctcatcagt tttttatacc catctccgat cgtctgttta
    38041 ttttccaaac tcttttctaa atattggatg gcttgatcgt tcacatacaa ttcagaaaat
    38101 ttaagtccaa cttgttcata aagtttcgct aatttatgct tatcttgggt agtggaaatc
    38161 tcttgtttca attgtgtaat ctctgattga atcttttcgg tcgcttctgg cgatagggct
    38221 ttttcttctt ttgccagaac ttttttcggt tctttttctt ttttcgccga ttgtaaaatt
    38281 aagctagaca actaaaaaag tcatttgtgc tacactcaaa atagttccga ccaaagaatt
    38341 ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt gtaataatag
    38401 agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg aatcgttcaa
    38461 gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc ttagaatatt
    38521 atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt ttacccgagg
    38581 aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat gtgattgttg
    38641 gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg
    38701 ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat ggacatcaag
    38761 aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag gattatagcc
    38821 aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg aaacataaaa
    38881 gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg aaaccgtgtg
    38941 gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa
    39001 acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg aaacagatca
    39061 gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct caaagaggcc
    39121 taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct atggatttca
    39181 attcagtag
//
LOCUS       DACESX010000011        37878 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.011, whole genome shotgun sequence.
ACCESSION   DACESX010000011 DACESX010000000
VERSION     DACESX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.011"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            29..316
                     /locus_tag="IXF88_000476"
     CDS             29..316
                     /locus_tag="IXF88_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507231.1"
                     /translation="MINKKTGKRIFSSKDEVIRDLSENFEPIELFFHQLNLEIEKLLK
                     EPTEGRINKVLKLIACKDSHAYMLYPKWYADKNIAEINDYLASIKERNAIN"
     gene            353..640
                     /locus_tag="IXF88_000477"
     CDS             353..640
                     /locus_tag="IXF88_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507232.1"
                     /translation="MAEVKFGSKEWFEELNSMIQFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            731..970
                     /locus_tag="IXF88_000478"
     CDS             731..970
                     /locus_tag="IXF88_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507233.1"
                     /translation="MKELEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHFPKVRFYN"
     gene            1098..1427
                     /locus_tag="IXF88_000479"
     CDS             1098..1427
                     /locus_tag="IXF88_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507234.1"
                     /translation="MKLEGSLQNRMMEGRTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELIKTKNGWKVLKEDTYFYIGDRE
                     KYYDPSL"
     gene            1480..2328
                     /locus_tag="IXF88_000480"
     CDS             1480..2328
                     /locus_tag="IXF88_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507235.1"
                     /translation="MFSQEEIISKSQPDKNGTITMKKKIPCYRCSGSGQYKFMSMGKI
                     TYGTCFKCDGDGFLVVTEKSFTPEYRAKLNALNKKRADRKIRITREKESQKNFDRYGF
                     EKGYIYVVIEENSYAIKDELHHQGATYDPLLGWFFKEKQEQYKTQKVFVEEFSVINDE
                     GIIELKLEEYSERQQEILRAKRREEGEFIGTIAQRLELDLKLTNTVTIDTQWGNVYLH
                     LFEDQNHNSLIWKTNKFLYYDEWDVGEIRKVKATIKAHDYYRDTKQTWVKNVKIQKNE
                     SEGIKV"
     gene            2325..2642
                     /locus_tag="IXF88_000481"
     CDS             2325..2642
                     /locus_tag="IXF88_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507236.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            2645..2878
                     /locus_tag="IXF88_000482"
     CDS             2645..2878
                     /locus_tag="IXF88_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507237.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIFQRFRLPVSE
                     LKKMMRLSAYLLMFLNVVTLIFVVIGVTFNLME"
     gene            complement(2995..3474)
                     /locus_tag="IXF88_000483"
     CDS             complement(2995..3474)
                     /locus_tag="IXF88_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507238.1"
                     /translation="MTDKTSDLPKLAPKTSRVKRKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            3594..4931
                     /locus_tag="IXF88_000484"
     CDS             3594..4931
                     /locus_tag="IXF88_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1507239.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVSGDVAKNIDDIYKFMNGEYGLSGATIAGILGNWKQESGNDPRTVEG
                     YYGDGQREVEFIKQFTDGLMGIGYGQWTAGRHTALVNWAKKNFGGAWWTTEAQLDFMF
                     KGDGGFVAILKDYALNASDDVVANAVAFHKNWEISADSESAVVAVRGGNAKEIWEYMK
                     SKGMDGAKDVSKINKIAGGTSTGGGSNNSSSSASGTVEDACATTDDANSGSGSMGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            4954..5481
                     /locus_tag="IXF88_000485"
     CDS             4954..5481
                     /locus_tag="IXF88_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507240.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSDKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            5468..6031
                     /locus_tag="IXF88_000486"
     CDS             5468..6031
                     /locus_tag="IXF88_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507241.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLENLDSYVRAE"
     gene            6454..7191
                     /locus_tag="IXF88_000487"
     CDS             6454..7191
                     /locus_tag="IXF88_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507242.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(7208..7624)
                     /locus_tag="IXF88_000488"
     CDS             complement(7208..7624)
                     /locus_tag="IXF88_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507243.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            8016..9263
                     /locus_tag="IXF88_000489"
     CDS             8016..9263
                     /locus_tag="IXF88_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HAQ1507244.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            9533..11923
                     /locus_tag="IXF88_000490"
     CDS             9533..11923
                     /locus_tag="IXF88_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ1507245.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     KFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNTLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            11972..13807
                     /locus_tag="IXF88_000491"
     CDS             11972..13807
                     /locus_tag="IXF88_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1507246.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            13882..14739
                     /locus_tag="IXF88_000492"
     CDS             13882..14739
                     /locus_tag="IXF88_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1507247.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            14729..15838
                     /locus_tag="IXF88_000493"
     CDS             14729..15838
                     /locus_tag="IXF88_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1507248.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            16177..16977
                     /locus_tag="IXF88_000494"
     CDS             16177..16977
                     /locus_tag="IXF88_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507249.1"
                     /translation="MVEISKTTLDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            17065..17871
                     /locus_tag="IXF88_000495"
     CDS             17065..17871
                     /locus_tag="IXF88_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507250.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            18103..18972
                     /locus_tag="IXF88_000496"
     CDS             18103..18972
                     /locus_tag="IXF88_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1507251.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            18992..19339
                     /locus_tag="IXF88_000497"
     CDS             18992..19339
                     /locus_tag="IXF88_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507252.1"
                     /translation="MEIISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            19352..20614
                     /locus_tag="IXF88_000498"
     CDS             19352..20614
                     /locus_tag="IXF88_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507253.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            20694..22070
                     /locus_tag="IXF88_000499"
     CDS             20694..22070
                     /locus_tag="IXF88_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ1507254.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            22067..22696
                     /locus_tag="IXF88_000500"
     CDS             22067..22696
                     /locus_tag="IXF88_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507255.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            22715..23194
                     /locus_tag="IXF88_000501"
     CDS             22715..23194
                     /locus_tag="IXF88_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507256.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            23696..23749
                     /locus_tag="IXF88_000502"
                     /pseudo
     CDS             23696..23749
                     /locus_tag="IXF88_000502"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23753..24352
                     /locus_tag="IXF88_000503"
     CDS             23753..24352
                     /locus_tag="IXF88_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1507257.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIHFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            24368..24844
                     /locus_tag="IXF88_000504"
     CDS             24368..24844
                     /locus_tag="IXF88_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507258.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            24861..25763
                     /locus_tag="IXF88_000505"
     CDS             24861..25763
                     /locus_tag="IXF88_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507259.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQANNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            25836..26030
                     /locus_tag="IXF88_000506"
     CDS             25836..26030
                     /locus_tag="IXF88_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507260.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            26135..26563
                     /locus_tag="IXF88_000507"
     CDS             26135..26563
                     /locus_tag="IXF88_000507"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507261.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNKETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            26798..27385
                     /locus_tag="IXF88_000508"
     CDS             26798..27385
                     /locus_tag="IXF88_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507262.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            27385..28284
                     /locus_tag="IXF88_000509"
     CDS             27385..28284
                     /locus_tag="IXF88_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAQ1507263.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            28284..29198
                     /locus_tag="IXF88_000510"
     CDS             28284..29198
                     /locus_tag="IXF88_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507264.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESGMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            29250..29465
                     /locus_tag="IXF88_000511"
     CDS             29250..29465
                     /locus_tag="IXF88_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507265.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANRLY"
     gene            29655..29909
                     /locus_tag="IXF88_000512"
     CDS             29655..29909
                     /locus_tag="IXF88_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAQ1507266.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            30088..31137
                     /locus_tag="IXF88_000513"
     CDS             30088..31137
                     /locus_tag="IXF88_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAQ1507267.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            31316..31546
                     /locus_tag="IXF88_000514"
     CDS             31316..31546
                     /locus_tag="IXF88_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507268.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            31549..31806
                     /locus_tag="IXF88_000515"
     CDS             31549..31806
                     /locus_tag="IXF88_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507269.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            32023..32898
                     /locus_tag="IXF88_000516"
     CDS             32023..32898
                     /locus_tag="IXF88_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507270.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQENLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSEKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKEE
                     ITKMKPVIKILDNKE"
     gene            32908..33702
                     /locus_tag="IXF88_000517"
     CDS             32908..33702
                     /locus_tag="IXF88_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507271.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNNGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            33731..34849
                     /locus_tag="IXF88_000518"
     CDS             33731..34849
                     /locus_tag="IXF88_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507272.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKVKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTVNEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFAKSKDEVKGVVIDDDFEFDFD"
     gene            34882..35202
                     /locus_tag="IXF88_000519"
     CDS             34882..35202
                     /locus_tag="IXF88_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507273.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            35459..36148
                     /locus_tag="IXF88_000520"
                     /pseudo
     CDS             35459..36148
                     /locus_tag="IXF88_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
     gene            36185..36352
                     /locus_tag="IXF88_000521"
     CDS             36185..36352
                     /locus_tag="IXF88_000521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507274.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            36372..>37878
                     /locus_tag="IXF88_000522"
     CDS             36372..>37878
                     /locus_tag="IXF88_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507275.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLN"
BASE COUNT        14349 a         5118 c         7697 g        10714 t
ORIGIN      
        1 tcaaaatgga gatgaaatca gcgcgtatat gattaacaag aaaacaggaa aacgtatttt
       61 cagcagtaaa gacgaggtaa ttcgggattt gagtgaaaac tttgaaccaa ttgaattatt
      121 tttccatcag ttaaatctag aaattgaaaa attactaaag gaacctacag aaggacgaat
      181 taataaagta ttgaaattaa tagcatgtaa agatagtcat gcttacatgc tttatccaaa
      241 atggtatgca gacaaaaata tagctgaaat aaacgattat ctagctagta taaaggaacg
      301 caatgcaatt aattaacaat agacgaaaaa tgaaattaaa ggagatagga aaatggcaga
      361 agttaaattt ggatcaaaag aatggtttga agagttaaac tcaatgattc agtttccaga
      421 aaagttcact ttttactaca acaaaagaga caatatcatt caagtagctt tacttgaaac
      481 taagggaaga ttagaaaata ctcgatggag tatgataaaa ccatcagcac tcagatttca
      541 tcctaaagac ttgacagcgg ttgtagcggt aaaagaggtt caagactcac aagaatacta
      601 tgaagtagta aaaaatctga aggatatctg gtgtaaataa ttctaaattg accagttatt
      661 tggtcaaaaa tagtatgtga ttatttcatc caatatggca tcataattgg tatgaaaggg
      721 gataaattat ttgaaagaac tagaaggtat taagaaaaga aatcaggaaa gattggaact
      781 gttaaatgaa aatagaaata ggatgtttag ttactatgaa tccatgaggt gtttaaataa
      841 atctagaagt gaaattatag aatcatcaaa gtataaacaa ttgatggaag agagagtttc
      901 tctcattcaa gaaatagaaa cagagtctat ttttccagat cattttccta aagtccgatt
      961 ttataattaa acattaacaa atgaaacgat gctaaatagg catcgttttt tatttatttg
     1021 ttaaatttat ttatttacaa aatgtgtatc agtgtgtata atatatttgt agataagtta
     1081 agaaaggatg atctgaaatg aaattagaag gtagcttaca aaacagaatg atggaaggca
     1141 gaacaacaaa taaggagatt gtagttggta tgggagcaac cgagcttcta tataccgaca
     1201 gaaatcctta tacagtgatt gaagtaaaaa gcaaaaacag aattttggtt caagcggatg
     1261 gggcaatcaa taaagctact tttccagatc aagagtggga atacagcaga aatcctgaag
     1321 gtcaaatatt ggaattaatt aagacaaaaa atggatggaa agtgcttaaa gaagatactt
     1381 atttttatat aggtgataga gaaaaatact acgatcctag tttataaaca aattgaagat
     1441 gagcgaggtt ttatcctcgc tcagaaagga gaatgaagga tgttcagtca agaagaaatc
     1501 atcagcaaat cgcaaccgga taaaaatgga actataacca tgaagaaaaa aattccttgc
     1561 tatcgttgta gcggatcagg tcagtacaaa tttatgtcta tggggaaaat tacctatggc
     1621 acatgcttta agtgcgatgg tgacggattt cttgttgtaa ctgaaaaatc atttacgcca
     1681 gaatatagag ctaagctaaa tgctttaaat aaaaaaaggg ctgatcgaaa aataaggatc
     1741 acaagagaga aagaatctca gaaaaatttt gatcgttatg gttttgaaaa gggttatatt
     1801 tacgtggtta tagaggaaaa tagctatgcg atcaaagatg aactgcatca tcaaggagct
     1861 acttatgatc ctttattagg ttggtttttc aaagaaaaac aagaacaata caaaactcaa
     1921 aaagtttttg tagaagaatt ttccgtaatc aatgatgaag gaattattga attgaagcta
     1981 gaagaataca gcgaaagaca acaggagatt ttaagggcga agcgtagaga agagggtgag
     2041 tttattggta caatcgctca acgattggaa ttggatttga agcttaccaa taccgtcacc
     2101 atagatacac aatggggcaa tgtttatctg catttatttg aagatcaaaa ccataattca
     2161 ttgatttgga agacaaataa atttctttat tatgatgaat gggatgttgg agaaataagg
     2221 aaagttaaag cgacaataaa agcgcatgat tactacagag ataccaagca gacttgggtt
     2281 aaaaatgtta aaattcaaaa aaatgagtca gaagggataa aagtatgaaa agagaggaat
     2341 acaaacaaag attaaacgaa ctgcttgaag aggacgaaac attaactcat ggttcgcctg
     2401 atgaaattct atacatgatt gataatatgg tgatctttgg tggatatgaa cttggtaata
     2461 ggtcagttga tcataatatt ttagaatttg atgatgtatc atgggaagaa attcttgatt
     2521 ggggaatttt ggcggtacct gaaactaaaa cctatattag tgacactatg gttccttttt
     2581 ttgaggaatt ggattataaa agacttccca aaaatgaaaa tcatatactg ggaggaaact
     2641 aacaatgtta agtgacttat ttattttact gatctatcta ggtgtaagtg aaattattgc
     2701 gtattttttg tttgtcattg ctaattatgg tatttccagt gcaatctttc aaaggtttag
     2761 attaccagta agtgaactaa aaaaaatgat gagattatca gcatatcttt taatgttttt
     2821 gaatgtggtc acgttaatct ttgtagtaat cggtgtgact ttcaatttaa tggaatagag
     2881 ggaaatctat aatggaaaaa agagaaaaga atgaacatat ttttttgaat caatattgtt
     2941 gatggttttt gcgatcagcg cttaccagaa atctaaaaaa agagtaatga ttgtttaatc
     3001 atcactcttt ttttgtaggg aaattgaaat atgtggagct tgtattggtt tagttaggat
     3061 tgcttggcta taaggagcta gttctttatc aaaaccatca aagtaggttt tcagtagcga
     3121 tttaactgat cgctcatctt gtttgatttg gataatttca gcatccaaat cggttgtgag
     3181 atcaggagct tttgatgcga ttacctgatt ttttttatct tccaactctc ttaggctgtt
     3241 taacaagttg taaaaagaat cattttgatt gttcattcca acgtgagttg caattttaac
     3301 agaagtttcg tccgtattgt catcaaaaga actcattgga atatcaagta gtaacgagga
     3361 tgtttctttt actgactttt tctttacagc attttttgtt tttccatctt tttttctctt
     3421 tactcgagat gtttttggag ctagtttagg taaatcactt gttttatcag tcatttatta
     3481 gcttttcctt tctattaatt tatgttatta tattatcata ataacataaa aaagtacact
     3541 ttttgggggc gtagttattg aaaaaatcta agagaaagat tgccacgttg cttatgttaa
     3601 tggtgcaatt tacagggatt gcaatgagtg taatcacaat aaatccgcaa gtgatacatg
     3661 ctgatgggat agctacttat gatgcacaat ttaactgtac tactgaaggt agtgggtcag
     3721 gaacagatag cgatggaaac gatactggaa gtggcgctgt atcaggtgat gtagcaaaaa
     3781 atattgatga tatttacaaa tttatgaatg gggaatatgg gctatcaggt gctactatcg
     3841 caggtattct aggtaactgg aaacaagaat caggtaatga tcctcgaacg gtagaaggtt
     3901 attacggtga tggacagaga gaagttgaat ttatcaaaca attcactgat ggtctgatgg
     3961 gtattggcta tggtcaatgg acagcaggtc gccatactgc attagtaaac tgggcgaaga
     4021 aaaactttgg tggcgcttgg tggacgacag aagcgcaatt ggattttatg ttcaaaggtg
     4081 atggtggatt tgttgctata ttaaaagatt atgctttaaa cgcttctgat gatgttgtgg
     4141 caaatgccgt agcgtttcat aagaattggg aaatttcagc agattccgaa tcagctgtag
     4201 tggctgtacg tggtggaaat gcaaaagaaa tttgggaata catgaaaagc aaaggcatgg
     4261 acggagctaa agatgtaagc aaaatcaata aaattgccgg aggaacaagt accggtggtg
     4321 gatcaaataa ttcatcaagc tcagcatcag gaacagtaga agatgcctgt gcaactacag
     4381 atgatgctaa ttctggttct ggatcaatgg gtaaagaagg aaaaattaac ggtaaaaaag
     4441 gtcaagtaat agcgaatatg acttatgaag aagctttgaa aaaatatggg aaagcgatca
     4501 cgctaccaaa attcaaagaa ccagattggt caacttcacc ttttaacgga atatatcaag
     4561 gtcagtgtac agagttgacg tgggcttata tgagccaatt atacgatggg aatcaaccga
     4621 ctactggaaa tggtggctac gtatgggaaa gctacaaatc ggcaggagcg aaaataacga
     4681 acaaaccaat cgtaggttat gggtttagtg cttcagatgg atggttatgg gctgttggcg
     4741 atgcagggca tactggtgta gttatagcgg tgtttgaaga tgggtcattt ctatgtgcta
     4801 actttaatgt tccaccaacc caagctccaa caaggggagt ttcggtaact ttgattgatg
     4861 gagtggacgg atcagataat gtgcatttct ttagcggtgt aggaaaagct aaatttgaaa
     4921 tcgaaaaata aattggaatg aaggacgata tagatgaata gtaaagtgaa agatacaatt
     4981 atcatttctt tgctttctgg tatgttatta gcactgcttg ttggaatcct attcttcaat
     5041 aagcagaatc aggacataga agctgaaaaa cacagtgtgc atattgaaga taagctgaca
     5101 ttctttaaag aggttaaacc aaatgaggtg gatggattac ggaaagattt agaaaatgat
     5161 attcaagaaa tgttgaatgg atcagttcaa aataagtttg cagatcaatc aggtgaagaa
     5221 tttttgagaa aactatttat tgtcacaggt aaaccaccaa ttactgataa gagtagtgat
     5281 aaagaaaaaa tcgagtacta taagcctttt acttttaagc taacaaactt tggaattaag
     5341 acatctagta gtgggttgta tgaagcattg accgattttg aagttcaata caatggaaaa
     5401 aaagtgtatg ataccacatt aaccatcaca ctaaataatg ataagaaagt gactgggggt
     5461 gctttttatg caaaatcatg aaatcactac tgctaaaaag gcgattggaa tactttccat
     5521 aattgtgatc ctttgttgtg tggtactatt tcaaaagaaa aatgcttatg ataataatgt
     5581 caaagaattg gaagacctta aaagtaaaca aatcgaagta gtcaagaata accagaaagt
     5641 gaaagctatg caggaagccc aatacaaaga attggggaat gctgaaatca aggaacaaag
     5701 taggaaattc tttaatttat tttacaactg gaattcatgg gaaaaatata cgaagaatat
     5761 gaaggaaata caaaaagaat ttcctcagat agtaaagtct agtcctgttg atatttctgg
     5821 cacgatggta ggcactggta atagtccaat atctagctat actgctgatt actatacaac
     5881 aaatcaaaaa ggtaaaatgg ttgaattgat tacacagaca agaaatactc caacaacagc
     5941 aacaacaaca atttggcgtg gagtaacaag cataggagaa aatcaattgt tttacttgga
     6001 gaatttagat agctatgtaa gagcggagta aaataaaaaa agagctaacg gtaccaacgt
     6061 tagctctttg tgctgacgat gataacaaaa cctaccgaaa aaggattgtt tcatcacaaa
     6121 cacaaacaga aaagaattga taaaaaaccc gaacctcaga atttttatta atcaattcat
     6181 ttagattata ataaattgag ctgaacatta caatacgtat atgttgtaaa acgtaaaaaa
     6241 agtcaaaagt aaatactttc ttaaaaagtc aaataaaact attaaagaca atatatatgg
     6301 tttatttgac ataagaaaat ggtctagatg ataattgtaa tatagaattg taggcttaaa
     6361 taaagcataa aatacctttt atgataaggt atagctctta attttaagca gtttttagat
     6421 ataagagtga aagtaaagtg ggggagaata ataatggcag atcgtaaatt agaaaagtta
     6481 ttagaagaaa cgtggaatcc aaaagaattt agtgaatttt ttatggagaa ttttgaaact
     6541 gacttagcag tgattgttaa agatgctctt agagagcaag ggtatcctga aactgcaaac
     6601 tatataaaca ttaattttac tttgtatact gaaaataaag gaacttggga tttttgggct
     6661 actttagcaa ataaggagtt aagtgataaa tcagacacag gaattcgtaa cttttttgaa
     6721 tcaaacagag atgattatat gtatgctaac catcaagata aattgaactt tagagtagag
     6781 tttgatgaaa cacctgaaga atttatagaa agacaaccgc ctaaagaaaa tgtagcaaag
     6841 gtattggaag atcgttggaa ttcagatgaa atagtttcaa caattagcga actaggcgga
     6901 caatatgaac cgctagtaga agcggttaga gaagagctaa gactgaataa gtttcctgat
     6961 gttcaaaata ttgatgtttc tcaaattgaa ataaacgtca agatcacgaa taaacttgat
     7021 tatggtagtt gggcagatat agcattagaa aaatacatct actcaacgtt aaaagagttt
     7081 atcgaaaata gaatggatat catgtatcta cagcatccac agtatttaaa ctttggtgtt
     7141 gaaatagcta cacctcttga agaatggaaa atggagcaag ggcttgacta gcctttgctc
     7201 cattttttta ttttacaaat gaatacagtt cgtcgttttt accaagaatg attgtttgcc
     7261 caactttgag attgtcaggt gatccttcag cggtttgtaa ggagaacgtt ccgttgaact
     7321 tatcggatac cgttataggt agctttaatt ctgatccttc ctcatctttg agaattgctg
     7381 tttttcgttc atcaaaataa acagtcttta ttaaataatt tttcgtagtg atctcagcag
     7441 atttgtcttt ttttgtagta gtaacagttt ctttagaatt agaggtattt tctacgacta
     7501 ttccatcttt ggcatttgag cttgtgttta tttgattgaa tattttcagc gacaagatac
     7561 cgctaagaat agtcaatata gaaacaagtg taattaatac tgataatttc ctttttttgt
     7621 tcattttttc ttaacttcct ttttgttttg attagttaga tattccttat ctaacaagcc
     7681 tttttaggct attaaatgag gataaacccc aaaaaaatat tgcaattatg acagtgcaat
     7741 ggtactatta ttatacaata aattttgcga aaggggtagg tctaaatcgc aaacaaaaag
     7801 aaattgaacc tagacaaata caacttgtac accgcaaggg aagcaagttt gattttgggt
     7861 aaaggtagaa attatgttac aaacataatc aactattatc caaatcgttt gcctaaaggt
     7921 tcgttcaaga aagtgggtga caaaacctat attataacgg aagaaggaat agaagcattg
     7981 cgatcacaaa cagaaaataa aaacaaaaag aaggaatgaa tatgaagaaa aattcgcttg
     8041 gtatggtttt atttgcatca gctgtacttg cggggttatc agtaccagta acctcacatg
     8101 ctgatgaatg gactgcaaac acaccggatc aaatccaaat tacaacagga caatcaagtt
     8161 atgcgttcaa aaaaggagat actatttggt caactgtaga gaagcttaaa agcttaggtt
     8221 acaagactac tgttgctgag tttgccgaat tgaacaatgt tgatttggca aaaggagaac
     8281 aacggactat cccaattgga cgaactttca acatcagtaa agatacatta acagttaagg
     8341 aagcagatgg cgaagtagtt agtcaaactg ttcaaaaaga taaacctgat cctgttgaaa
     8401 atacggagca agagaacaaa aaatccgaga ctgcaaaaga atcggaacca acagcaaaca
     8461 cttctgataa gacagaaaca agtgcttcaa gtgttgatgg agcaagttca tctaatacaa
     8521 ataattctac aagtgagaaa gaagcatcac agggaacaag tgaaagtagc actacggaaa
     8581 ctgatactgg aacatcaaca aataactcac aatcaacatc aaatgatgca aatgaatcaa
     8641 ccagtacatc ttctagtgat caatcacaaa caggttcgag tgatgaaact gacagttcta
     8701 cttcaccaag taaacccgaa caaccaacta atgttgtgct aggcgttcca tttattagtc
     8761 aaggtaacac aatgctttgc gaaggaacat ctttgctaga agcgttacat tataaaggtg
     8821 ttgcaacaaa tcaagattta atgacttttg ttaaatcaat gccactatct cctgataata
     8881 atccaaataa tggtttttct ggtgaatgga gacacaatgt ggatggaact tatcaaggta
     8941 tgaatccggc tcctgtagtt tcatggggtg ttgctaatgg cggaaatgtt gtagatattt
     9001 ctggttcatc ggtacaagga ttaaaagatg agattgtaaa tggaaatcct gtagtagcgt
     9061 gggtaactta tgcctttgaa cctgctcaac ataaatcaat gttttggggc gatgctattt
     9121 ggaatcgaca tgtagtagcc gttgatggat ttaaagacgg tttttaccat gttgtcgatc
     9181 ctgtgtttgg ggcaagttgg attaatgcag gatcatttga aacagcatat aacgtatctc
     9241 aattagcggt ttcggtacgt taataaaaga gattgtttat atactaagta gttaagtata
     9301 gtctttattt gtccttgctt aactacttag aaaacttgtt gctcatggtg ggtagaacta
     9361 aaaggggtga tgctatgaat actagagcgt tgaaacagct tgctttcaac gaaaaaagtg
     9421 attaataggt gtggtgattt taacttagga atgggcgcga aggtcattac cacaccgaca
     9481 tatcgtggct cattttcttc gcctaactgg tataaagaag gagtaacgag agatgaagca
     9541 tgaaaaaacg ataattttaa aatcaaaaat aatttattgt tttgcgactt tagtaatggt
     9601 gttatctacg cttataatgc ctttttcagc aataagagct gaagcagatg tcacaaaacc
     9661 gacaagtgca aaatttacag aaactggcgt aaaaacagac ggttatatta gggttaatat
     9721 gccgaatcat ccaaatgaat ggatgatttc aagtcaattt aaagattctc atggaaatat
     9781 tggatattgt atggattcag aattgccatc cccaacagga agtggagccg gaagcttgaa
     9841 gtacaaggga gctggcagtg atgagttcta tcgtatgttt aaaggtggtt ttccatcaaa
     9901 aacagcaaag gaactgggtg caggaaatga tacagaagca tggtatgcaa cacaattagt
     9961 ttcatgggtt ttagccggaa actttaaagt ttcacagatt gtatggtcac atccaaacca
    10021 taccgccgca gaaacagctc gtgtaaaaaa agcatttgaa aaaatttatg actatgctaa
    10081 gaatggaaaa gatacgccca acactgagtt ttctatcaca gcttctaaga cagctgacga
    10141 aggaaaatat cacactttca catataaaac agcatcaaat aagactggta atgcgaagct
    10201 cacttttaca tcagcaaaac cagctggtat gaagatttat gatgcagatg gtaaagaaat
    10261 tactaacaac actgtgaaat taaattcaag ttttactatt aaagtacctg tgactacacc
    10321 aagtggtact ttgtcattta aaggtactgc taatgttagc acaaccaatc cttttacttt
    10381 tgatggacga ggcgtgtatc aagatgccgt agttatgatc actacttcag aaacaaagga
    10441 ctctaaatct ttgagtgcta aatggacaag agctgttggt caggtaacag ttaagaaagt
    10501 tgatcaagat ggaaatgctt taaaaggagc ggagttcact ctaactgaca ataacaatgc
    10561 taaatctgtt aagacaaccg gagatgatgg gttagctacg tttgatttgc aacaaggctt
    10621 aacatacagc ttagaagaaa caaaagttcc tgaaggttat caaggttcat ttaaaaagga
    10681 tggtattact gttaataccg atggacaaaa atttagttac actgctacga acacaattaa
    10741 aaaaggtaaa gtaaaagtta aaaaggttga ccaagatgga aaaccattag ctaatgctga
    10801 atttaccttg acggatgatt catcccaaac acaagcagaa aaaacagatg aaaacggact
    10861 tgcaagcttc gatattgtag caaacaagac ctacagcctt gaagagaccg gagtaccaac
    10921 tggttataca ggcgcattca agcaagaagg aattaccgtt aaagatgatg gacaaacttt
    10981 tgaatataca gctacaaaca ctttgaaaaa aggtgtagta aaagttcata aagtcgatca
    11041 agatggaaaa cctttggcaa atgcagagtt cactttaacc gatgaaaaag gtgcaacaca
    11101 agtagaaaaa acagatgaaa atggacttgc aagcttcaat attgtagcaa acaagacgta
    11161 tagtctagaa gaaaccggag taccagtagg ttacacaggt gcatttaagc aagagggtat
    11221 cagtgttaaa gacgatgggc aaacttttga atatacagct acaaacactt tgaaaaaagg
    11281 tgtagtaaaa gttcataaag tcgatcaaga tggaaaacct ttagcagatg cagaattcac
    11341 cttaacggat gaaaaaggtg taaaagaagt taaaaagacg gatgcaactg gtctggctac
    11401 gttcaacatt attgcgaaca aaacttacaa cttagaagaa acaaaagtac caacaggtta
    11461 cacaggcgca ttcaaacaag aaggaattac cgtcaaagat gatggacaag tctttgaata
    11521 tacagctaaa aacactttga aaaaaggtgt gatcaaagtt cataaagtcg atgatcaagg
    11581 gaaagcatta gcaaacgcag aattcacttt gacggacgac aaaggtgtaa aagttgtaca
    11641 aaaaacggac agaaatggct tggcaacatt taatattgtt gccaacaaaa cgtacaaaat
    11701 cgaagaaaca caaaacccta atggttatac agggaaatgg tctaaagaag gcatcaccgt
    11761 tgctgacgat ggacaaacct ttgaatatac agtaattaat caccttgtac ctgaaacagt
    11821 aagaacaggc ggaatcggta catcagcctt ccttcttgta tttctaattg caggattgag
    11881 tatggtgcta ctgtattatc gagaacagca caaagcaaaa taaaaaagtg ttaatggaaa
    11941 agaagtaaat atgaaaaaag aaggctgacg tatgaaatca aaaaaaattt tttcaaattt
    12001 aatgctattt ggtactttgc taggagtggg attgggcgca agctcaatct cttctagcct
    12061 tggactaggg gggcaagtag catatgcaca acaaaactat aattatcaag gaactggaac
    12121 gattcatatc cataaagtaa caaatactaa tggggatggg aaccctgtct cagatattga
    12181 taacaaaggg aaagaaatag ctttaccaaa tggtgtaaaa cctcttgaag gcgttgagtt
    12241 ctcttatgtg aaacttacta aagatgaagc taaatccttg ggatggacgg atagtgatac
    12301 tacaaaaggt caacatattg atgatgctaa agcacgatta ttactaaaca aaaaatacag
    12361 tgaagtaagt agaatgaaga ctgattcaca agggaatata gacataccta tacaagtaaa
    12421 tacagacgat ttaacagata acttgtattt gattgttgaa acaggaacag tcaaaggtgt
    12481 agttgaagaa gcgtatccta tgattgttaa cgtacctgaa ctaaacggat cagatgatca
    12541 atactttaat tatgatgtac atgtctatcc taaaaactat ttccaattaa actcagcaga
    12601 gcttgataaa aaagacgaag atggaaaggc tcttgccgga actgtttttg aattgcgaaa
    12661 agatgatgga tctgatcagg ttgttggaac ttataaaaca gacagcacag gtaaattaac
    12721 tgtggatggt ttggaattag gtgactatta tttcctagaa accgatttgg gaacagatgc
    12781 taatagaaca acctatttac tagatggtaa aaaattcaaa tttagcgtta aacgtgggga
    12841 gactgctaaa gtagttcttg atgttaccaa ctacaaacga cctgtaatta ctaaggtaat
    12901 tgataaagaa accgttgata ctggcgaaga tgttcaatat acattgaaac catcaattcc
    12961 tggtaatatt ggtaattacg aaaaatatgt tgtcaaagat gttctaggtg cagagttaac
    13021 ttatgattca tcagaagtga tcgaaggaat gactttagat actgattaca gtatcgaacg
    13081 tgacggacaa acgacaaact atgtctttac tgaatcaggt atggataagc ttcagaaaca
    13141 atttgatgca ggggatcgct tctttacaat tttgatgaac gctcatacta atagtaaagc
    13201 taaaatggat acaccactta agaatgatgt ttcaacgatc tacaacaatg gatatgaacc
    13261 cgaagacgaa acaccagttg atcacgctta tacaatcatt ggtgatcacc aattcattaa
    13321 aacagatgga gatacaagca aagcattgag tggagcaaaa tttgtagtga agaacaaaat
    13381 tgatccgaat aaagttactt acctaaaaca agatccaaca acgaaagctg ttacttggat
    13441 tgcagataaa tctaaagcta ctgaattcgt ttccggatca gacggtaagt tccaaattga
    13501 aggtttgcaa tatacctatg atgtagattt tgataaacaa ggtaatccaa caatcacaga
    13561 taaaaaaatc aatgattatg ctcttgaaga agttgttgct ccttcaggat atacaccaac
    13621 tagaaacgat gttccatttg tggtatcgaa tgatgatgct tctagcgaag taacagacat
    13681 caagaacctt aaaacacctg aaacagttcg tacaggtgga attggaacag tagtatttct
    13741 agtggttgca ggtgtggcag gaacagggct atatgttgtc tacaaacgag aacaacacaa
    13801 agcttaatga aataaaattc cctagagtgt ttttggctct agggtttctt tttaaaaagt
    13861 gtgtgaaaag taggtatgag catgaaaaag aagaatatca agttgatcat cttaaaaatt
    13921 gggatgttgc tatgtttagg agtaggtcta tatcctgtaa ttactcaggc ttatacctac
    13981 taccaaactc aacaaacact aaactcctat aaggaaaaat atgatgaagc ttctgaaaag
    14041 gagaatgaag aagtcttcaa aagatattca gaagttaata ggaaacgaaa agacacagaa
    14101 caaacctcag tcacgattcc tgaagataat aacaatattc cttcaaatgg aaatcttcag
    14161 aaggataaat taggtgaatt agtgggtgaa attcttatac caaagattaa tgtggatttg
    14221 ccaatttatg atggcgtaaa cgattttcaa ctatcagcag gagcaggaat attaaccgga
    14281 actgattatc caactggtgg gattggaaac cattctgtta tatcagctca ttcaggcatt
    14341 cctaataaaa aatattttac agatttagag ttgctcaaac ttggtgataa attttttatt
    14401 gatatttcag ggaaaaaact tgcttatcaa gtagatcgga taaaaacaat tctaccaacc
    14461 gaatttgagg atttgcttcc tgtggcagga aaagattatg taacacttat gacatgtaca
    14521 cctgttggag ataattctca taggttactc gttagagggg ttagcatacc ttaccaaaaa
    14581 agtattgttg agaaagaaaa gaagaaagaa gatttaaagg taaaggcgca gatgaactgg
    14641 tatcctatcg tagttgtagg ggttttatta ataatagctg tgatagtcat tattaaaaaa
    14701 atagtagcaa agagacgagg aatgaaaaat gaagaataaa atactacttt ttttgactct
    14761 tatttgcact ttggttttaa caatttatcc tgcaaatcag ctctatcatc aattcaaaac
    14821 aaaagagaat gtggcggaaa tccaagagaa aattgagaaa aaacaaactc cttcaaaaga
    14881 ggaagtggca aaaaaagtcg aacaaataaa atcgaacagc ggtaaccaag gattagacaa
    14941 tactcagaaa acagataaag gagtagacaa tactcaggaa acagacggtg gatcatttga
    15001 tattcgtgaa gctgaaggaa aggtcaatcc atacacagga gatgtttttt caaaaaatca
    15061 aacgaaagat tttgaaccat ctacctattc aggagcggtt ggttatgtct atattccaaa
    15121 aataaatgtg ctagagcttt tgtttctagg cgcttcacag gagcatttat atagaggtgt
    15181 ggcacaaatg agtgctacta gtctaccttt atcaggtaaa ggtcatcaaa cagtaatagc
    15241 aggtcatcgt ggcggttata cgtcaccaca atttctttat gttgatcaat tagtagccgg
    15301 agaccacatt tatatagatt atcttggtga aaaagcagtt tatacggttt tccgaacaga
    15361 cgttattaga aattacggtc agaattcaac actgactgat attcctgatt tagatatgtt
    15421 aacactttat acttgtcatc catatcctca gaactacgat cgtttattag ttagaagtct
    15481 aagagatgaa aaagcaaaat acatcacgcc acaagtaaca gttggcaata ccgcatacgc
    15541 tgatgccatt gatagtgaat ccgtttcaga taagccagaa actgttacat cttctaatga
    15601 tttatttgag gatgttgatc gtgccaacaa ggaattagat ggaaataatg atcttaatat
    15661 tccggaacaa ggtagcgtag agatgcaaga cagaacgttt gctgaaaagg caagttcaat
    15721 atttacagga attaccaata aacatcagaa aatgctttta gtcatttgga ctgtattagg
    15781 agcattagca agtctaaagg ttcttattct tttaatcaaa gagtttagaa aagcataatg
    15841 aagattataa aaattgatag agtacagtaa aaagggattg atactattat tgatctcttt
    15901 tttattttga atagagctag attcactttt aaataaaatc aaaactgatc ttaattccaa
    15961 agaaaaagtc aagaaaaaaa tacaacaaat ttttttatca gaatatttat cagaatatta
    16021 ttagaaaaga taaactcaaa agcttaattt aatagggtta ttggttgtat ttagaaataa
    16081 tgtatggaaa agctataaag caatagataa gatgtagaaa atcttatttg tgaatgaaaa
    16141 acaagtcatc gtaaaatatt aataggaggt gtagtaatgg tggaaatatc aaaaacaact
    16201 ttagactcat tagctaaact atttagtagc aggtttgatg gtgcagaatt tgttgaatat
    16261 ctaagccaaa acgaaagtgg aaaaactctg atcgaaacac tgaaaaaaga ctatttgaat
    16321 gaaaatgact ttccgacatg gacgaatatt gaagatgcta catgggatgt agtgtgtacg
    16381 atatattctc cttgggtaac gattgctgat tttgttttag ataaaattgg aggggataca
    16441 taccctgatg ccaaatctct tgctacaagt gactttaaaa cgttctatgg ttatctaatg
    16501 ggtctaatag gcaatggaat agatttgaaa gttacgccaa aacaaacacc tgaagaaatt
    16561 gaaaaagaaa aaggagaggt tcaaatggct tatgcagatg acagagaaaa agtaaaatta
    16621 ttggaaggta aattatctga agaagaactt ggaaaaatgg tgcaagaaat caatgaagac
    16681 agcaactatt ttcctgaatt gaaggtatat caaaacgacg aagatttctt taatgaaatg
    16741 tttagtaata gtccatggga agcagtgagt aaaacaaatt taggagaatg gaactttcaa
    16801 gatgatctag ttgcatttag caatgatggg tatttacgat catattcaga aagtgagtat
    16861 agagctgaat taaaaacgta tgaaacggat attctgatga gctatttaaa tctattagag
    16921 agtggtcagg gagagttaag agataaagtt gaagaactat taaaagaaga gctataggga
    16981 ttatttcaac ataaacacaa gcagaaaact accaaacaaa acgtaccatg caaaaagccg
    17041 gttaaaccgg ctttttgctc tattatgaga ggagatcaaa ccatgagtgc aacaaccgat
    17101 atggctttag aagcagtaat aaaagataat acaggaaatg tcgaaaatgt agaagacttt
    17161 agcgatattg caatcaatct ttcagcatgg atttcagctg taggagaaga aaatatcgaa
    17221 tttgagatca agaatatgga tgacttaaaa gcaaaatttc cttttatcgc atcgaaacta
    17281 agtcaatttg ccgaggagaa atcgtggaat gaattagtta gtttttccga aaaaatttat
    17341 gatattacgc aagaagatga gtgtgtttca tgggaaatga ataatcaata caagaaaatt
    17401 gatgctttcg tcaaatcacg tatagaagga acagcttttg agtttgaagg tatagatacc
    17461 gcagataatt ggttaaataa agaatccttt attaaaagta tagcggatga tcttgaaaaa
    17521 caatggtcta aggaagacta tgcagagtac ctaaaaacag aaagagatac agaggtgaaa
    17581 gaaaaactca ctgaggtatg tttggatgaa catattgatc ccaatatcgt aaaacaagaa
    17641 gactatgaag tggttattga taaagttcct actgattttg aggaagttgt aaagtatata
    17701 gttgaagaag cattatcgca atactcttca gatgaggaag tggacgaaga aactgttcgt
    17761 accagagttt ggcaactggt ttttgatgac aaagattata aatatttgag tgatcaaaac
    17821 tcggttaatt ggagtgtacg attattaatt gatcaaaatg atattgaata aaatagagct
    17881 gaaaagctct tttttttttg ataaaaaaac tagctatttt tttaaaaata atttggttca
    17941 aaatttattt ttttaacttg ataatttttt gccatccttt tttaaattgg ctatatatca
    18001 aggtttatag tggggataaa taaaaataca tttgacaaaa tttacaatat agtttattat
    18061 atatttatct tattatgata acacaataaa aggaatgatg gtatgaaaaa gaagaagttt
    18121 agtcgatcgt tgttaggaat tttagtttta gttgcggttg gtcttagtgg caagggttac
    18181 tttcagaata acagcactca ggaacatgat tttcagatca aaagtagtgt tcaggtaggt
    18241 aataaagcag acagtgaatc aaaagaattt ttagggatac gttatgatcc aaacaaatat
    18301 ccaaataata tcgtaaacat taatgatggg aaatatacac tgacaaaaga agaccatgaa
    18361 gcattatcag aaaaaggcag tcaaaccttt tgggtaaatt atgcagattt agatcaactt
    18421 ggaagagcag gagatgttga agcgttgatc acctatgatg ctgtaaattc tcactcatct
    18481 aaaaatgtgg aacgtccatc tttcaaaagt tctgtccata ttgcaggaga atacaaggat
    18541 ggagtatttg atcaagagag agatacttgg gtcggcaaag attcaaacaa tgaaatcact
    18601 cagctagaag gatataaagg ttatctctat aacaaaagtc attcattagc atggtcgcta
    18661 ggaggagata tggaggtaca caatttaaca cttggaacga gagcgcaaaa tgttgggaaa
    18721 aatgatcaga atggagggat ggcttactca gaaacgattg tacgtgatgc aatctataaa
    18781 aataagaatg aaaaagtatt gtataaagtt gatccaattt ataaagggaa tgaattggtt
    18841 cctcgtggtt ctcatgtcga agctttctca gttaacgata atggaaaaac ggtaaacctt
    18901 aatgtttatg tatttaacaa tcaaaaagga atcaacatca actatcaaaa tggtacttgg
    18961 gtaagagatt agaaattgag aaggtgaaga attggaaata ataagcatca atggattaga
    19021 taaatcagtt gcaaatcgaa taaaaaaagg aaatggctac tatcttctga agaagggtaa
    19081 atattcagtt gctagagagt tagaaacaat gagtatcgtg tatgtagcta agaatcattt
    19141 tgacgtagaa caggctattt ttgatccaga ttctgaaata gggaaatgtg ttgaattatc
    19201 agagaaaact gttagtacaa aaggggcgat gatcattgag agagaaatgt tagacgaaat
    19261 cagagtaatc attcctaaag atacgcagat gtgtgaggac agcataagtg acgatgaacc
    19321 aaaatcagaa agaaaataag gagatattca gatgagcgaa aaatccattg aattaaagga
    19381 atataagcga agcgatggag aaaaatatta tcctgtagcc agtttttcta aatcagtata
    19441 tctggttgga cgtgcattgg tagttggtgc aattggcata tatattttgc attgggtaac
    19501 aaaggttgta caactgcttg tagatacctt agtaagtatt cttgtctcgt taaatgatgt
    19561 taacggaaca gatgatttag gattaaaaga aatgtttaaa gagttactca atgcgttttt
    19621 tcccaattat ttaagttatt catataatgt acaaagcttt tttgcaaagc tacacttggt
    19681 agacatctct attttagtat tagaagcagg aacggtattc tttgttggat atttggttac
    19741 tttgatgttc aggcatcact atggagaaca aagaccattt tttgatgata tggaaagtag
    19801 aaggttgaaa agagaagctc tagaagctat ggatgtgaag tatgatccaa acaagattat
    19861 aggagatgaa acgaacaaca aaaaaagaaa acgaaacaaa acgcaattag aatccgatgt
    19921 gctatcaacg cttaaaaata tcaaaatttc tgttcataca agacgtgatc taagtgtcaa
    19981 tgaagatatt cgagaatata tcgtaaaaat cccgcaacca aaagggaaga aagttagaga
    20041 aacagtttta cgacaaattc aagatttagg gtcgatcctg acaagagcaa caagcggacg
    20101 agccaaattt ggtgaaaaag aagatgaagt ttcaggcgat tatttcattt tccgagcatc
    20161 agtaaatatt gaaagagaac gtaaggaaag agtaagtacc aaaaacaaag aaacggaaag
    20221 agcaacatct tcaatcgcag tttcaaattc acaattttct tttccaatca atctctttgc
    20281 cgataattcc aaaaatatcc aaaaccaaac cgaaaaagct agaaaatatg ccgaaactca
    20341 aaagcaatct ttagaacttt atctatcttc tgctgacgta cctgcaagtc taacagatat
    20401 gcacgttggg aatacatcta ttgaatattt gtttacttta tctcgtacca gaaaaattca
    20461 actaagctca atgaaagaag aaattgaaac ttacttgcat atatcaggtg taatcgtgcg
    20521 gatcaacgca gggcgtattc aaattacttt accattagga gaaaatgatc gtattccaat
    20581 tgatgtacca tcaatgatca atagcgtgtt ttaaaagaca tcacaaatag aaacagaaaa
    20641 tacaaactac cagttcacaa acagaaaagt accgagaaaa gtgaggaata gaaatggcta
    20701 tgaaattatt agagaaagtc aaaaagagag ccgaaaaagc tgaatctatt ttgctagata
    20761 aagaacctaa tataaagctt aatggaaaat tcagtgtcaa tatgttgaac acaaaggcta
    20821 aagacccgac agatgctatt tttggaatta agcctgatgg cacaattgct catttttgca
    20881 tcagcgaaat gccacacatg ttggttgcag gaacgacagg atcagggaaa tcagtattac
    20941 tgaatgaaat tcttgttaca gcaatgtgtc actcaacgcc tgatgaatta aaaataggaa
    21001 ttgtagaccc aaaaagggtt gaatttggaa gatacaaaaa attgccgttc atgttggctg
    21061 atccaattac agatatggat gaagcttatg atttttttga atatcttgtg atcttgatgc
    21121 atgaacgtta caaactgatg gaaaaagcag ggttacaaaa catcacgcta tataacgaat
    21181 atgccgaaaa aaatggatta gatagatttc cctatgtgat cttattagtt gatgaatatt
    21241 cacagttggt tggaacacat aaagaagtag aaggtttgat tgttcaatta ggacaaatgg
    21301 caagggcagc aggtattcat gttatcttag ctacacaatc tcctcgatca acagttgtca
    21361 ctggtattat caaagccaat tttccgtcac gtgtttgcct tatggtagca agtgatttag
    21421 aaagtaggat cgtattagat gaaggcggtg gagaaagcct aagtccaaag ggagatatga
    21481 ttattaagct cgttaatgga agtatggtta gagcgcaggg agcttacatc agtaacaaag
    21541 aaattgaaac aattttcaat caccttagaa atactatgcc agaaccagaa tatgttgatt
    21601 accgagcaat cgtagctgaa tctcgtggtg agggtggaga agacgaggat tttggtggaa
    21661 ttggtaaagc tcctgtaaga aaatctgcta aaagaaattt gaacacacct ttagcaaatt
    21721 catcatctaa agagccatca attgaaaaga agagtacgtt tgctctaaaa cttgaaaaaa
    21781 acaaagaaga aaaagaaaaa caaggagcta aaacactaga aaaacttcaa gccgaaaaat
    21841 tagaaaaagt tttaaaagca ttagataaag ctgattctga accgcaaacc tcaaacaaca
    21901 cgaataatga acagccgatt tcatctaaaa ctaaaccgaa aactactgat ccgaaagttt
    21961 cagtaaacgt gaccatcaca accgataagt ctgagaagaa acaagtaaac ggaacgactt
    22021 cacctatcat gcaaaaaatg ttgaaagcca aaaaacaggt gcataaatga tcattgtcag
    22081 ttcttttttg aggtggctta cgccatataa atatacagat gaagtagttg tgaaacgaac
    22141 gaagaaaaga gataaaaaag cggtactaac tcctaaacag atgcttgatc atggcatttt
    22201 aggggtgaag gttttagaag tgaataaacc gtttgaatca aatagtgcaa gagaaaagtt
    22261 gcttggagct gaagccgatg tcgcatattt ttaccatgac aaaagcaagc atcaaatagc
    22321 tattaaattc aatccaaaat cggcatggcg gtatcaagca agagatgact tgaaacgtag
    22381 gaaacttaga ccaatgctct atccagaagg aaaagaagca tttgatagaa cccacatcgt
    22441 tcccattggt tttcatggta cagaatcaga taaccgccta ttagtaggtt ggaacagcaa
    22501 tcaaaataga gttcagatca ataaatttga gaagaaagta aggaaattca acgatcgaaa
    22561 aacgatccta tggtttgcag atatttgtaa acagccggat ggaacagcaa agtgggaagc
    22621 ggtgatattt gacgaaaatg gtaaaaaagt aatgtctgaa acatggcatg ataaagctaa
    22681 atttgtttgg cagtagcagt agaggtgaaa aagattgcta gaactttttg aagcaaatag
    22741 aacaggcttt aatcaaaaat tagaagtggg agaaactata gaatataaag ataaaaacta
    22801 tattattatc ggaatctata acacgagaat taacagaata ggtgatccac gtataaccag
    22861 tgacttggtt tgtcaatctg ttaactattc acctgatctg acaagcaggt ataagaaata
    22921 cgtgttatta gaatatagac acaagattac tgatgaaatt ggtgtaaaca acacacgaaa
    22981 catatttaag atcggaacag tgattcctta tagtaacaac gaagaaaaaa tattttatca
    23041 gatttacgga atagaaaagt ttgaatatga acatgtcgat ctgctagtga cgtaccaaat
    23101 gcgtttaata gaaccttggt cacaggcaga aattgataaa gcagtaaaat tgaaccgatt
    23161 atcaaaattt aacgtattag gaaatgcctt ttaaaaatcg caatacacaa acagaaaaat
    23221 accaaaaaat tagcaatgaa cgtaggtaag aacatgacaa taaataataa taaccctaaa
    23281 aaatataatg ctcttgtcca atgctatatc gaaaacaaaa ttacaatgca agaagtcgct
    23341 gatgaattag gcaaatcaaa gagcctagta tcacacgatt ttaagcacaa tgtttttccg
    23401 aacccgattg agctgaggaa aaaatttcaa aagagagttt atcaagaaat aggtaagtgg
    23461 attgatgaag ggaatgcaat tgacccttta attgaaaaat atcctttttt aaaaagcaaa
    23521 aataaaattc aaaggttaat tcgtcaaggc gatattcaag ctaaagaacc tgttttgaca
    23581 accaatgatt tggctaaaat aaatcttcat aaacaaatta ttgaagactt tgaaaaaaat
    23641 ctttcaacaa ctgaaatttt aaaaaaatac aatattccga gaggaaagct aacatcgact
    23701 ctttttagag aatatggaga atcggcagtt actaaagcaa aggaagtagc gaatgagaga
    23761 aaaaaacaaa aatagtagtc gatacctagt agcgactgta gtttttgttt gtgtattgat
    23821 tttcctgttt gattttttgc agattggaaa aagcataatt ggaaatacat tgagtgcaaa
    23881 ggaaatggtt gctttaggtg gtttgtatga aggacacttg ttcccgaata tattcatttc
    23941 tatcgtttct catattaact tgtaccattt aatttctaat ttattgttct tagtagttct
    24001 tgaagaagca ctgaatgatc agtatgagtg gttaacatta ttgatcgttt taatcatatc
    24061 aggatttcta ggaagcttag ttccttttct gctacacagc aacatcgtaa gtatggggct
    24121 tagtggaggt atttttggat ggattgcttt atttgcgatt gcatacatcc attttgatag
    24181 caagaaacat ttattactaa gtgtttttgt attattgata atcggcattt tactggtatc
    24241 tacttttgaa tcaggcgaga caaacgtctt gtcacatgta atcggatttg tctgtgggat
    24301 gatcttggga tttattctaa agaacgtttc attgatcaaa caaaagaatt aaaagggagt
    24361 gctaaaaatg ccaaaaaaaa aagaaaagaa aattccagtg tttttgtctg tctcagaaat
    24421 gttatcactt caaatactgt taagaaaaaa gattgaaaca atagacgaag aagttagaaa
    24481 taaaagggct atgtacgaac aacgtgaagc gctaagagaa cgaatccact attttgatga
    24541 tatacacact aaaatctatg aagcacgtac aaatcacgaa gacgaaagca aattaggatt
    24601 agctcaacaa cgaatatttg aatggctgaa tacgaatact gagcaagaac cgttaagagc
    24661 aattagtaat ctttttgaat ttggaacgaa gcattggttt aacgctgtag aaaaggatgc
    24721 tgacttacaa gaagcgttcg atacactaag cgaggatagc aaatccatgc gagacttatt
    24781 gatcacttat ctaaatgagc atgaatttga acagacgaaa gttaaaaaga aaaataaaaa
    24841 atagaaagtt gtgatgtatc atgctgttta ccttattagg ttatttatgt ttagttcttg
    24901 ctttttttgg agcaatagct atatttgtcg gtcagtttca agcaaataat ccgataatgc
    24961 gtaacatagg cattggaatt gttatcatat ccttgattgt atttggtgcc acaaagattt
    25021 ttgggagtaa tgatcagcag tttggtattt ctggagttga taatagttat gctccagatt
    25081 cattgggaag agtagtgata gatggaacgt ttgagaatgt aacgagctac aaaaatgttt
    25141 cattgacagt gaaagaagat gacaagaaag catcagatga cgttctcgct acgatcaagc
    25201 cgtctcaatc acagtttgaa gtgttttata cagtcaaaga gaataaccct gtacgcaagc
    25261 taacattagt ttttaacgtt gacggagaag aatactataa agatgtaaaa atcaataaag
    25321 catcaacgaa aaagattgtc gaatcagcga aaaaagaagt agctacagaa gaaagtacaa
    25381 ctgaatcaag ttctactgtc gaagttgaac taccagataa aattgatttt gatcctgaaa
    25441 aagtcaaatc gtttgaatct gtttctggtt ctatggcaga tcggtttaac actgaagttt
    25501 tcaaagctat caaaactgtt aaattggata ctgaaaagca aaggattaat gttaccttat
    25561 tcaatgcagt tgaatctatg gatcaggata aaaaagttac ggttgcaaga gaactaggga
    25621 aaagagtaat tgggttgtat cgaggtacgg caaaaccaga ttcagacggt tcagatttgc
    25681 cagttcacgt agtttatgaa gacggtaaag tttttggaga ctctacagca aatgaagcac
    25741 taacgattaa attagctaaa tagccggaaa attataataa acaagtggtg tcacaccacc
    25801 atcgcatagg tgtgacacct tggagatgat tactgatggt agtaaataaa gaaaaagtaa
    25861 gaaagtatgt tactttaaaa atagcttctg ttgaatattt agaaaagcga gctaaagaag
    25921 aagaaaaaac aagaggtaag aaggtaacag tttcagagtt gttagaagaa ttaattgaga
    25981 gtgaccaaac aataagacgt gcatttacca ataggtctgt taaaagttag ggtgatacta
    26041 tctttaattt caaccaaatt tctaatatat gatgaatagt ttccttgtac aaaatatcta
    26101 attaagagat aaaagttagg aggattcatt caaaatgatt atccaaaagt ttgactggaa
    26161 aatgaaatta ggaattagtc ttgtaatatt gggatttatt atttttttgg ggataaaagc
    26221 acaaaaaaat caatctactt ttgaaactat gtcttctcaa attattgagg aatgttccaa
    26281 aatttattat tgtgagaaca aaactgaaga atctaatggc ttttattata tcgatcaacc
    26341 acatgatgtt gttataaaaa tggagtcaca tgaagtaaac gaatggattc aaagaagtac
    26401 taatgtatca aaaaagttag aaagatattc tcaaacgttt ttaagtaaag tcattttttc
    26461 aaattgcaaa aataaagaaa cgtctcaaag agtagctagt tctttagaaa atttaaaatt
    26521 ttctgtttca aaaaaagaga aatttgtaga tttacgaaaa taaactatcg aaagtagtat
    26581 gattatgatc ttaaaatatc atcttaagag aagaattacg attgtttata ggatttacaa
    26641 aatgaataag tttgaagagt gatttggtcg cagactagat cactcttttt gtataaaaaa
    26701 actttacact tatttttgag tgtgttaaga ttttttatat agagtgacgg tttcttgtca
    26761 gagtgaatga taaccatcaa tattggagga ataataagtg gaaaacaata atgatattag
    26821 atattttagc attggggaaa gagttacaac agtaagtgaa ttatcaaagg attatagaga
    26881 taaagaagga acgattattt ctcttaaaaa gaatcgaaga ggtgtttggc aatatgttgt
    26941 tgatttaggg gatggcgtta cagttgagtg taaagatagt caactgatca gaaatgatgg
    27001 cagtcattcc attgaaaaag caattcttgt attcccaaaa gtatcaagca agattatgaa
    27061 tgactggttg cttggaaaat ttggtgagta caataaaaaa gaaatcgcag aagtgatcgg
    27121 caaatcttat ccaacagtat tagggtatct aaaaaagata acgaacaaag aagcgaaagt
    27181 tgaggatgtt ttaacagtaa gagaacttca gtcattaatc gaatatcgga ataaacaaat
    27241 aacaaatcct gagtacctta aaaaagaaga tggtctattg aaacaattgg aacaggtaaa
    27301 taaaatccgg aaaatgcaaa aggtagctcc tttaagccta gataacttta aaaaaatcta
    27361 tttgaaagag atcgaaaaag actaatgtgg agtgtcactt taccgtttca acctgtatct
    27421 gcaccaagac caagtgcaag aaggaatgtt gctgatgcaa ctaatgacgt agcagagaaa
    27481 aggatttcaa cctattatga taaaaagtat ctcgattatt tgaaaaaaat aaaaagcttt
    27541 atcgaagatc aaggtttgtt agatgatgaa ttttactcga tcgtaaatga tccgatgggt
    27601 gcaattatgg aagtggactt ttactatcag ataccaaaaa gctataaaaa agtctataac
    27661 attatgaaga cgacagcgcc agatttagat aatttagtaa aaagtgctat ggatggtatt
    27721 ttcaacatct cagctatacg agatagccat atcacagggt taattgcttt taaatacaat
    27781 gtggcaaacg aaggaaaaac agttgtgagg atcaagcgat attcagaatt tgagtgggat
    27841 acttctgaag gattgaacgg agatatttgg gaagccacat ttgatttcaa accagtagca
    27901 acgccaagac caaagtctaa atttagagga aatgggatta tcacttacta tccaaaagag
    27961 tataacgact atttggaaaa tatcaagaat acgcttaaag agaaagattt ggtaaatgat
    28021 caactgaaaa aggtaatgaa tgctcctatg ggggttatag ctcagataga ttatttttac
    28081 caagtgagaa aagataagaa aaagcttgat tcactgatgc gtacttcaca gccggatatt
    28141 gataacttag tcaaaggaac attggacgga atttttaaca aggaaattgg catcaaagat
    28201 agcagggtgg taggtttaat agcttttaaa tacaatactt ttgaaaagtc gaaaacaaac
    28261 gtgcgactac aggagatggg ctgatgaata ataacgaaaa aattcttcat gtacttgata
    28321 gctttgaaac aattcaagag gaactaaaga aatatcgaga tgttttggaa caaagatatg
    28381 attttgtcaa tcagcaaaag agtaatcaca tggatttcat tctcaatatg aatgacttga
    28441 agaagaaatt agttgaacga aaagaacaag aaaagttgat caaagcttac tttgaactcg
    28501 gtgaaaaaga agttaaaaag gcaatggaac tgaatgaaga tcgcagggtt ttagatcagc
    28561 tgttagaaca attattggtg atgtttcaaa aaggacgaat agatgaggat ttaattgaag
    28621 aagggttgag aaagtatcct gcaaactcag gtatcggaat agtgctgaaa gcgattgatg
    28681 aagaagaaat tgaagatttc ataccggcag aggattttga atcgggaatg gaatatataa
    28741 aatattacag ccaagggatc actgctttta gagagtttga tcctgaagat gttattcatg
    28801 acttgaacaa cctaaaagaa tggtgtgaag gttatgaagt tgatgattcc ggtttggact
    28861 acttgatcag tattatggaa atcgaagaag agatgccgga taagcctgat ccaacagata
    28921 ttttagaatt aatccatgat gctagaaatc caattgcata catttctaga ggatatactg
    28981 tattggaata ctataagcct tatatatcag cgatgaacca tttaagacgt gtattaaggg
    29041 aaaaacgtga ataccgctca gtgttaaatg catcgaatag attagaaaaa gctgtttcag
    29101 agctagatgc ttattatcgt gaacattacc tgcaagcagg tggtatgcca agaaatacga
    29161 aagcaaatat cagtaggtat ataaaagaag ctgagtaggc ggagttagcc cataaaatgg
    29221 caaaaggtaa cctgaaaggg gcggtttgaa tgaaaaggga tgtttttgaa ttgtacgtat
    29281 tagaacgtct agaagacggc atgatggcac aagagataat tgatgaaatt gatgatatga
    29341 tcagcagatt tggtcaattg gaattgaatg atggaaacaa aaattcggta gtgctaaaac
    29401 aattttggtt aaataatttt tcgtataagg attttatcaa agaaaaggca aatagattgt
    29461 actgactaaa ttcacgagga tcagtgatga gagcttattg ctaagagcag ttaggctctt
    29521 ttttatttgt gtaaaattat tttattttac aatacacatt tatacacaaa aaatgttgac
    29581 ggatagtaca caaatgtggt aggatatatt tgtaagcaag ttagattaac aagttaaagg
    29641 aaggaagagc agttatgaaa aaagaagaac taatagatat gtttcaaatt gttgaacgag
    29701 caaataatat gggaattatg ttttttgatc gtatttcttt aaagatggat ttaagcgttg
    29761 cccaccaaga atttaatcta cgattaaaag ctttattgat ttctgatgat gttaatttcg
    29821 ctcatgatgt tgtaggtata cagaaccata tagatagaga aaacaaacgt atgggtgatg
    29881 gattcttacc aagatattca agtttatgat ttggcgaaag attcaagagt atgtgtcatt
    29941 attgataggt actcttgaat tggaaaggag gaatgaattt aagacaacaa cgattatgat
    30001 cttaaaacaa tcgtttatgt aattggcaat cttacgaata attttcatgc aataatgaca
    30061 ataaaaaatg aaaaggggta aaatcaaatg aaaaaatttt tagtaggaat tctattcaca
    30121 atggggctag tgctattatc accttcagtt tcagcagaaa gctctatttc catgtatcgt
    30181 atgtataatc ccaatagcgg agagcatcta tacactagaa gcgttggaga aagagacaat
    30241 ctgaaacgtt atggatggca ttacgaaggt gtcgcgtggc aagctccaac aagtggttcc
    30301 ccagtttatc gcttgtacaa cagatataac ggtgaacatt tttacaccgt tgatgccgga
    30361 gaaagagata agatttcgca cacaggatgg acgtacgaag gtgttgcgtt ctattcctac
    30421 aacggagcaa atagagttcc tgtgacacgc ttgtacaaca aatacgtgaa ttggcaccat
    30481 tacacgttag atcaaaatga aaagaacgta attagtaaac aaggttggaa gattgaaggt
    30541 acgggttggt atgcaatgcc aaacagcgca ccaaatccta cacctgcacc tgctccaagt
    30601 aatgaaaaag tgttaaatgt tcctgtcatt tggcaaggta atacgatgtt gtgtgaaggt
    30661 gcttccctgt tagagggatt gcactacaaa ggagtagcga caaatcaaaa cttaaccagt
    30721 tttgtaaact atatgccaag agctaatgac aataatccat atcatggcta ttctggcgaa
    30781 tggcgctaca acgtaaatgg tacgtatcaa ggcatgatgc ctgatccagt agttaaatgg
    30841 gcaaaaaatg ttggtggtaa tgccgttaat atcactggtt caggaacgaa tgggatcaag
    30901 aatgaaattc gcaatggtaa tccggttgta acttggatta cttataacta tgcttcacct
    30961 caattcaaac aaatgccttg ggggcgtgca gtctggaatg gacatgtggt tctcgtggat
    31021 ggcttcaaaa atggtgcata ccatgttgtt gaccctgtat ttggcgcaaa atggatcaat
    31081 gcaagcacgt atgagagagc atttaatgtg actggaatgg cggttgctgt acgttaattg
    31141 atagaattaa taaataaatg aaataagagc ttaattactg aaatggtaaa tgggctctta
    31201 ttttttagaa aaaaactgtt tttttgtaat acgaatctat aacaaaaaaa cgttgaatgt
    31261 agtacacaaa agtggtatat taaaaccgta gagattatgc gaagaggtga ttttgatgaa
    31321 ccaaagaaga agaaaaaggc tagaagaaat aagcgagcga ctatccgagt taaaggatga
    31381 gttagaagta attcaagaag aagaggaaga gtacaaagaa aatatacctg aaaatctaca
    31441 gggatcagaa agatatgaaa aagcagaaga atccgttgat caacttgaag aagctgttga
    31501 tggtttatcc gacatattag aattgattga agatatagct agctagagat gattaatgaa
    31561 gatttcagga gtgagcaaaa aatgattgat atttcactga tagagaagct acttttttca
    31621 aataagtaca cagccaaaga gttggctaca agatggcaaa tatccgaatc agctattaaa
    31681 gcctatcgtc aaaataaggg aagcgtagga tggcgtgatt ggaaaggaat cagtattgga
    31741 aaagcagaag acataatgaa acgtgaagga tttcaagctg acaatgattc taaaaaagtt
    31801 gaatagttta atagtcgcca agaaaagtac caggacgagg ggaaaggtag attacaaagg
    31861 aaaacacttt taaataaact tcgcaaaagc atcaggttat gagtggctag ggctatacga
    31921 atgtgatgaa aacaaagggc tttaatgtca aagaaaacgc aatagggggg gaataaaatt
    31981 tttaaaaaag atatttatgt ttggtttaat ggaaaaaaat atatgactac taacaaaagg
    32041 tggtcagagc tagtacaaaa tacagttaaa aaagtcatgc cagatggata tcatttcaac
    32101 tatatacaat gctcacaaac aactgaaagt atttattttt caatattaaa tgagaataga
    32161 ctctattatt tcagggtaag ttcacaccat aggaaaaaga gagaagataa tgttacgaca
    32221 tttttatcgg gaaaatacaa tggcttcaaa gagttaagtg aagcaattgg tgagtatcta
    32281 aaaagtaata gatttgtcag aatttctgac aaacatatat tgatcttgtt actttatttg
    32341 aataatagct tcaaattatt ttggagagac gacactttct actatattga gaacggcatg
    32401 gatatttatt ctggtatcaa cggcatatta aaacattata ttaaggcgtt acttcgatat
    32461 ggggttcttt atcaagaaaa tttaagtaac caaattaaga ttagtaattc tggaaagtca
    32521 ttactggcaa aatataaaaa attgctttct cctatttaca aaggaataga tatggaagct
    32581 gtaaattctg aaactttatt taatttagtt gttgatgaaa agtttgcgaa tgaatttttt
    32641 aaaagtgaaa aaattgaaag actaaataac ctgtttcctg aaaatgcaat aaaagcttat
    32701 gtagataata ataaatcacg attatacaac tggtttattc ccaaacggtt gcgaggaaaa
    32761 atcaaaattg gcgatcaagt gaaagtaatt aatctaaatc atactaagat cgttaccgta
    32821 acggaattgt atatagctga aaaagaagag attacaaaaa tgaaaccagt tattaaaata
    32881 ctagataaca aagagtgagt ggaatatatg gtagcaaaaa agaatactct aaaagaagct
    32941 tctgaaaaat atcaagatat gaaaagacga ggattaatac caaaggacaa aaaaggtata
    33001 tggagagaat ttgttaagga agtcagtagt gatcgaagtg gcggtgactt tgttggacga
    33061 tatacagcaa aaggttatag caggcgctta aggatcactg ttgaatatgg aacgaatgaa
    33121 tcagctacag agctgaagca aaggtactta gatacacgaa gagaagcttc aaaaaaatat
    33181 gatgatttca agagcagagg tctaattcct aagaagaaaa aggggatttg gcgtgagttt
    33241 ttcaaagaag aagatagcaa tcggaataac ggagacttca ttggacgata cacagcaaaa
    33301 ggctacacta agaatttagg gatcaaggtt gaatacggaa ctggtgaaag ttcatgggaa
    33361 ttgagaaatc gctatttaaa tacattgtta gagaaatata aagcagaatt attggagttg
    33421 ttcgatgtta atgattttat cgagatggct gaaaagtatg gtcatgaaga aggttttatg
    33481 atcaatgaca ttatggttat tgataatttt acggatttat tagcacttgc gaaaaaagca
    33541 agggatttaa gagaaacgca gatgtatcta ttcagaaata aaaacggaag aaaccggaga
    33601 actgaatcga gtgagattga agattactac gatgccttta cacacgatct gggagccttt
    33661 gtagacgaga taagtggtgt aatgaacaaa tatagggtct agaataaaaa agccggagat
    33721 gagcagataa atgggaaaca atagcatata cacacctgaa atgcagaata aaattaaaag
    33781 acttgtcgga ttagggtgga tgacaagagg aaaattagat tcttttagta cgatcaaata
    33841 tcttttgatg gacatttacc gtcagaacaa gcaaacagga atacattttt tgggctatga
    33901 taagccggaa gatgcacctt tctttgttcc aagttcagcc tatagagcca gtacctccgg
    33961 tagcatgtat aacttggatt tgttcgagga aggagcaaag atgatcgaat atgatttcaa
    34021 cgaagcttat tcgaggatca tgagaaatta tcctttgccg tcaaacactt atttgaaagt
    34081 aaaatataat acagatcaag ctattttgag agcgataaat agaccaaaag taagaccgta
    34141 tgaaaattta aaaacgtata tgttcttttt ggttaatttt gaaggaacag ccaaagaagg
    34201 aacatatgct aatccaaaat cacagtcgat gtttgcggat tatggcgaaa atcccaaaac
    34261 agttaatgag cctatttggt tatcggagat tgagctaaca cttatccttg attattatga
    34321 tattaaagag atggaagtcc tagatagcca cacatttttg acgaaaaaag gattactcaa
    34381 agaatatttt gaaagaatcg agccattaaa aactgatcct gatacgttga agctttataa
    34441 aaatcttaga acgaaagtat ttggtgctat cggacaaaaa tcattaagag aaggagaaac
    34501 aagcaccttc gataaataca gtatgtataa tcgagcgtat tcctccatgg tagcaggcgt
    34561 atttagagat cgtatggtta gatatgaaca gaaatacgtt catagcgctt atgggctatt
    34621 aaaaattcgt actgatggat tgtatttcag atccgaagta ccagagtttg aagagctgta
    34681 taaacttgga gtagttaaga aaaaagttca tacaatatcc aaagaagaag tcgaaaaagc
    34741 tttagaattt aaaaataaag gatttgaaaa taagggctcc tttgcgaaat ctaaagatga
    34801 agttaagggt gttgtgatag acgatgattt cgagtttgat tttgactaag tactctttgg
    34861 aaaagatggt aggaggtaaa tatgaagaag aaaattgtgg aaggaaatgt aggtgttatt
    34921 attctaaaag attcaactga ctatgctaaa tcaatggcac taaaagacga aaaagttgca
    34981 cgcatggata aagatttagt tgaatttctg atggatgata cagaaagatt aaaaggggat
    35041 cagtttttga gtttaatgaa agacaaaggc ttaaacacat atggtttatt tgattataag
    35101 aaattagcta tagagtgggt aaaagaagga gaaatatttt ttattgaaga atgggaaggt
    35161 actgagaact tagttcttca tcagtatctt ttgaaagctt aatctaagta aaacagttct
    35221 gactagttat gcttgtcaga atttcaaagg atgttttcca taaccatcat tattcaaaaa
    35281 taaaggattt gaaaataagg gttcctttgg gaaatctaaa gatgaagtta agagttttgt
    35341 gatagacgat gatttcaagt ttgattttga ctagatttct ttttgcaacg tttaagagta
    35401 aacaaggtaa gaattaagat tgtgaagatt gagagtaaag aaaatagagg acgaatgtat
    35461 gagaacacac tatatttatt tagaagataa aattgtagat gaaattccgg caaaaagtcg
    35521 agaacaagct agaaaattat ttattgaaaa atacaaagag atcaatggag atagtgaagt
    35581 tccgaaagtg gtcgtaacag ttagaaaaag gcaaacaaaa aagagaagta cgtttcatgt
    35641 cagccctgat atatataatc ttatagagcg acatagaagt gaagataata tttctatctc
    35701 tcaatatatt gctacagtta ttgatgaatt gatttcagga aagtatgact atgcttttga
    35761 ttttgaggat gaaaaggatt acaaaagtgt tgctgtcttt caatctgatt ttgagaagct
    35821 aaaaaactat gctggcagga gaaatgttaa ttttgcaaga ctactagaag agttctactc
    35881 cgaagagttg ggagtaggaa atgaatacaa gagagaaact tatgcgaaat tgtctcataa
    35941 gttatcccaa gcgcttaaag aagtgacaga agttaaagcg gaactagatg ctaaacttga
    36001 agaagtgaaa aaatatcaaa cagagaggaa gaacaacatg aagaatcaag tggaatttga
    36061 cgaatagaaa gaatttgaaa aagcaagaaa acgctataac aaaattatga atgaacttag
    36121 ctatgaacac ttaacagttc atgaataatt gagtgagggt acagaaaaat atacgatcgc
    36181 agatatggtt aatgaagtgg aaactctttt agcttctttc tttgaaaatg gtcacgtaaa
    36241 cgatgaatgg cgaaaagaag aacctaaaag ggttaacgct tatattgctc gattaaaaag
    36301 gtttattgat aaatataagg agaatagtga caaattttct aaagataaat aaggagagag
    36361 tgtgattaca tttgccagaa atcgaaggaa tattttcgcc aaaaataggt aaaaaacagt
    36421 tagatttcag cagttttccg agcgtaaata gagattataa tctaggtgat gaggtacaga
    36481 tactaaatat tgatttcaaa gaactagact tcgctgttgc tgtttccaac aaacccaagg
    36541 cattggaacg agtgaaaaaa atataccaag agaatgatca aaatatcaaa aatatgatca
    36601 aaaagtatag acgagataat tcaaaagttg aagacgtttt attcaacggt acggcaaaac
    36661 atgatttgaa tgtttatatg gcattaggag tggctttgta ctgtaattca attaatcaga
    36721 cagatatttt ggttaatatc gtgaggaata gctatcaaaa aatattaaaa ataagtagcg
    36781 aaacaaaccc ttattatgca atgaaatatc ttggggataa tttattaaat gataaaaaat
    36841 tgatgcacaa cactgaagag ctgttatcta atttggctgc gattaacttt ctattcaaaa
    36901 caggttctga agattatttt atagataacg tactaaaagg tgcttacgga acagcatttt
    36961 atccgcttta caatatgaaa cagcataaag ccgaacaaaa ggataacaaa cttgaaatta
    37021 aaaagatgaa agaactatta ggtataggat ttgtaaaacc caattcaaaa gtcactattg
    37081 cttcgttagc aactactaac aatgatggga aaatagtaaa tgggtttgac gattcagata
    37141 tagatatatt gtcaaaatca tatttttctt tgtatggaga ttttgcagat ttgatgggta
    37201 ttgatagtta cttagtcaat aattttgaga tcaagggtag cgagctgaat gatattgtgt
    37261 atttcgtttt aatctctcta tatgcaattg atcagaatac cgagtaccga caaattgact
    37321 atttgctagt tttttatagt ttgttatatc agttcgtttt aactcggttg tttaaaaatg
    37381 ctaaggaaga attgatcctg aattctgatt atgaaatagc aaaacaaatt gcaagtatta
    37441 gaaaagaaaa caaatctgaa gtaatcaaat taagaaaaga attaagtatt gcgagtaaag
    37501 aaaacggtcg gttagaatct ttagtcaaat cggcaaaaaa agaagaggaa cttttgaagc
    37561 aagagattaa agaattaaaa ggaatcagaa aagaacttga acagctgagg tttgaaaata
    37621 aagtattaaa gcaattgcag gagcaagaac atagacaatc attaaatggt aaaactgaag
    37681 agccttcact gtctgaaatg atagcttatc taaaacataa aaacattctt ttagtgggag
    37741 gacatgtaaa ttttcaaaat aaattaactg aactattacc aaatattaaa atgtttgatg
    37801 taagcgaaaa taatggaaag atacttaaaa atttgaatac gtgtgatgcg atttttctat
    37861 atacagatta cttgaatc
//
LOCUS       DACESX010000012        35409 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.012, whole genome shotgun sequence.
ACCESSION   DACESX010000012 DACESX010000000
VERSION     DACESX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.012"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(2..532)
                     /locus_tag="IXF88_000523"
     CDS             complement(2..532)
                     /locus_tag="IXF88_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507276.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(631..981)
                     /locus_tag="IXF88_000524"
     CDS             complement(631..981)
                     /locus_tag="IXF88_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1507277.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1138..2667)
                     /gene="ahpF"
                     /locus_tag="IXF88_000525"
     CDS             complement(1138..2667)
                     /gene="ahpF"
                     /locus_tag="IXF88_000525"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1507278.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2677..3240)
                     /gene="ahpC"
                     /locus_tag="IXF88_000526"
     CDS             complement(2677..3240)
                     /gene="ahpC"
                     /locus_tag="IXF88_000526"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1507279.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3384..4046)
                     /locus_tag="IXF88_000527"
     CDS             complement(3384..4046)
                     /locus_tag="IXF88_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1507280.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4230..5876
                     /locus_tag="IXF88_000528"
     CDS             4230..5876
                     /locus_tag="IXF88_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1507281.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5867..6949
                     /gene="uxuA"
                     /locus_tag="IXF88_000529"
     CDS             5867..6949
                     /gene="uxuA"
                     /locus_tag="IXF88_000529"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1507282.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7057..8610)
                     /locus_tag="IXF88_000530"
                     /pseudo
     CDS             complement(7057..8610)
                     /locus_tag="IXF88_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            8758..9899
                     /locus_tag="IXF88_000531"
                     /pseudo
     CDS             8758..9899
                     /locus_tag="IXF88_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(10025..11104)
                     /locus_tag="IXF88_000532"
     CDS             complement(10025..11104)
                     /locus_tag="IXF88_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1507283.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(11207..11647)
                     /locus_tag="IXF88_000533"
     CDS             complement(11207..11647)
                     /locus_tag="IXF88_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1507284.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(11704..12114)
                     /locus_tag="IXF88_000534"
     CDS             complement(11704..12114)
                     /locus_tag="IXF88_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1507285.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(12294..12728)
                     /locus_tag="IXF88_000535"
     CDS             complement(12294..12728)
                     /locus_tag="IXF88_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1507286.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(12828..13676)
                     /locus_tag="IXF88_000536"
     CDS             complement(12828..13676)
                     /locus_tag="IXF88_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1507287.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            14065..15831
                     /locus_tag="IXF88_000537"
     CDS             14065..15831
                     /locus_tag="IXF88_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1507288.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            15933..17192
                     /locus_tag="IXF88_000538"
     CDS             15933..17192
                     /locus_tag="IXF88_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1507289.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            17339..18640
                     /locus_tag="IXF88_000539"
     CDS             17339..18640
                     /locus_tag="IXF88_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1507290.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            18643..19494
                     /locus_tag="IXF88_000540"
     CDS             18643..19494
                     /locus_tag="IXF88_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1507291.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            19507..20322
                     /locus_tag="IXF88_000541"
                     /pseudo
     CDS             19507..20322
                     /locus_tag="IXF88_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
     gene            20473..21639
                     /locus_tag="IXF88_000542"
     CDS             20473..21639
                     /locus_tag="IXF88_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1507292.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            21869..22516
                     /locus_tag="IXF88_000543"
     CDS             21869..22516
                     /locus_tag="IXF88_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1507293.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            22600..23544
                     /locus_tag="IXF88_000544"
     CDS             22600..23544
                     /locus_tag="IXF88_000544"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1507294.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            23679..25130
                     /locus_tag="IXF88_000545"
     CDS             23679..25130
                     /locus_tag="IXF88_000545"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1507295.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            25151..25987
                     /locus_tag="IXF88_000546"
     CDS             25151..25987
                     /locus_tag="IXF88_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1507296.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            25987..26646
                     /locus_tag="IXF88_000547"
     CDS             25987..26646
                     /locus_tag="IXF88_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1507297.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            26772..27461
                     /locus_tag="IXF88_000548"
     CDS             26772..27461
                     /locus_tag="IXF88_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507298.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            27467..28423
                     /locus_tag="IXF88_000549"
     CDS             27467..28423
                     /locus_tag="IXF88_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1507299.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            28467..28979
                     /locus_tag="IXF88_000550"
     CDS             28467..28979
                     /locus_tag="IXF88_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1507300.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(29235..29783)
                     /locus_tag="IXF88_000551"
     CDS             complement(29235..29783)
                     /locus_tag="IXF88_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1507301.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(29872..31053)
                     /locus_tag="IXF88_000552"
     CDS             complement(29872..31053)
                     /locus_tag="IXF88_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1507302.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(31289..32797)
                     /locus_tag="IXF88_000553"
     CDS             complement(31289..32797)
                     /locus_tag="IXF88_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1507303.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            32956..33147
                     /locus_tag="IXF88_000554"
     CDS             32956..33147
                     /locus_tag="IXF88_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507304.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            33350..34267
                     /locus_tag="IXF88_000555"
     CDS             33350..34267
                     /locus_tag="IXF88_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1507305.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            34260..35333
                     /locus_tag="IXF88_000556"
     CDS             34260..35333
                     /locus_tag="IXF88_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507306.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLLLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
BASE COUNT        11806 a         6425 c         6704 g        10474 t
ORIGIN      
        1 attaacgtat agtcattttt cttattctga tgaaattgat aatctcctgg atgcccatga
       61 aaatcaaaat aaagccgaat atttggaata gaagcatcaa acttttgaat ggattgaaca
      121 ataagacgaa tccgccaatc aacaacagaa tactactata aacactccaa cttttagctt
      181 ttgtattcat gctgaagaac aactggaaca agctgttgat gataacagct aatccaagaa
      241 gaatcggcag gatagaaacg attgcaggtg caaaagcata gacgatcaga gctaagatca
      301 ggaaaaaaag accactgaac agaccgatac tccaagaacc tgttttcctt ttaaaacggt
      361 aagcctcaaa aagattcaac aacccaagca aaacaagata ggcggtgatc agataaccga
      421 taaaatgaaa aacagcagaa gggtttagca caatcgcaat cccagaaata atataaacag
      481 cagctcttaa taaggcgtat ttttgaatat tttgtttaat cgtctcaaac atataagaaa
      541 acctcctttg acttatttta acgtgaacta gtcattttac caaattaaat gttacaaaat
      601 gagcgaagga gaagaatatc tctggaaaaa tcaacttctg aggatctttt ccattttttt
      661 gccttttgct agctcatcga tcattttatc taaataacga atttctttca tcaagttatc
      721 ctcgatgtct tctactcgta caccacaaac aacacctgta atcaaggaac gtgaaggatt
      781 ggggacaggg gcttccgcaa aaaattgttc gaaagtcact tgtttttgta gttgatattc
      841 tagttctgtt tgggaataac ccgtcagcca acaaatgatt tcatctactt cctcttttat
      901 acgatctttt ctctccgctt ttgctacata cataggataa acgcttgcaa aacttgtcgt
      961 aaaaatacga ttttgcgcca taatcagtca cctttctttt tttcaaatta tttactactt
     1021 atagtatata agaaaaagag aagaaaaagc gattattcaa aaaaacaaaa aggattgtga
     1081 caaaattctt gtcaccatcc ttttttccgc tctctgactt tttacaatct tcatagctca
     1141 agtgcgcatc agataatcga atgctcctaa agctgcagta gctcctgaac ccattgaaac
     1201 aatgatttgt ttgtatggac tatctgtaca atcaccagct gcgaaaacgc cggggatatt
     1261 cgttgcgcca tttttctcgg tcactatttc atttcttgga ttcatttcga tcgttctatc
     1321 tagccaatcc gtattaggga gtaagccgac tagaatgaat actcctgaaa cagctagtgt
     1381 atgcatctcc atcgtacggc gatcgatata ggagagagat tctacctgat tcgtaccgct
     1441 gatttctttc gtcgctgcat gtttgataac agtgacattc gcaagagaga ctagtttatc
     1501 ctgtaagatt tgatctgctt ttaattctgg taagaattct agaacatata catgttttgc
     1561 taacccagcc aaatcgattg ctgcttcaac accagaatta ccaccaccga taacagcaat
     1621 ctcttttcct ttaaataaag ggccgtcaca gtgtgggcaa taagcaattc ctttattttt
     1681 aaactctttt tcgcctggta cattgataga acgccagtgc gcacctgtcg cgatgatggc
     1741 ggtttttgct ttaagtgata taccgttagc taagccaata ttaataaaat gttgctcttt
     1801 atttaaggaa acggcctgtt gattcatcat aatgtcgata gggtaactac gcacatgact
     1861 ttctacttgc tgcatcaatt ttggaccttc tgtgtaagag gtaccaatca tattttcaat
     1921 ccctagtgtt tcaacgactt gtccgccgaa agaatcagcg acaagtcctg tacggattcc
     1981 tttacgtgct gcatagatag ctgcactact agctgctggt ccaccgccaa tcactaatac
     2041 atcataaggt tctttttcaa acaactcttc ttcaatcaaa gggccagtga ttttttcaac
     2101 aatttgttcg atcgtcatgc gtccgctact aaattcttct ccatttaaaa acacagttgg
     2161 cactgccatg atttttttat catcgatttc tttttggaac atcccacctt caatcatcgt
     2221 atgagtaata tttggattta atacagctaa tatattcaat gcctgtacaa cgtcggggca
     2281 attatgacaa gtcaagctaa cataactgtc aaaatgcaat gtttgtgtta tttttttgat
     2341 tttaagactc aaagattcac tgattttcgg tgcccgtcca ctaatttgta acatagccag
     2401 taaaaaagag gaaaattcat gaccgagggg gataccagca aaaacgattc cactttcttt
     2461 gccaactgga ttgatagaaa aactaggtgt gcgcatcgaa tctctttttt cccaagagat
     2521 tttaggagac atttctgcta tttccttcgt aaattcagct agtttttgac tattttcact
     2581 attatccaaa ctaacagaaa aaacaatagg agattcaatc agtgctaaat attgttcgag
     2641 attttgttta gtttctttgt ctaacatatc caccacctaa atctttccaa caagatcaaa
     2701 acttggtttc aatgtttcgc cactttcttt ccattttgcc ggacaaactt cacctgggtg
     2761 ggtacgtaca tattgagcag cgcgaatctt atcaatcagt gcactagcgt cacgaccgat
     2821 tccatctgca ttgatttcca tagcttgaac gattccgtct ggatcaataa taaatgttcc
     2881 tcgctgagct aatccttgct cttcatccaa aacatcaaaa atacgagaaa tctgatgaga
     2941 cggatcaccg atcatcacgt attcgatttt accgatagca tctgatgtat catgccatgc
     3001 tttgtgagta aagtgggtat cggtagagca agaaaagact tcaacaccta aagatttcaa
     3061 tgtactgtat tgttcttgta agtcttctaa ttcagtagga caaacaaaag taaaatcagc
     3121 tggatagaag caaagaatac tccagttccc cataagatct ttttcactga cttctaaaaa
     3181 ttctccttga tgataagcat gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat
     3241 agtaaaacct ccaataataa ttatttattt ataattattg taatttagtt ggagttgcaa
     3301 agtcaatgga tattttgtta tttaataaag attactcaaa aacgaaggtt aattataaaa
     3361 atagaagagg tcgcaagaaa atgctactgc tggaaatact cgggataacg ttgagtaaca
     3421 gtttcctgtt ctttcagcat catgtgtaag tgcaatcccg tttgaaatcg agcttcttct
     3481 ggttttttat caactaaagc cttgagtaaa tcataatgtt gttccatgat cgtttcccat
     3541 ggaagcccct gaatggtcac tcgtaaccag cggaaacgtt ctaaatgggt attatggtca
     3601 tctaaccagt tccaaacttc ttttcttcct gcaatttcga aacaagcttc atgaaataaa
     3661 ttatctgcac ggaaaaaagc gcgttcgtca tttattttga ctgctttttc ttgttcttct
     3721 aaaatcgttt ctagcactat tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg
     3781 atttgctctt ccaactgttc tcggacaaag cgagcatttt ctgcagaaac aagattaatt
     3841 ttggaaacat acgtaccact ttgaggtatc gtatacacga gttcttgctg acgtagttga
     3901 atcaatgctt cgcgaatggg agtccggccg atagcaagat gttcctccag tgccttttca
     3961 gatattttcc ttccaggttc taagtcagca tagataattt gtttacgaat cgcttgataa
     4021 gcttgtgatt gcaagctgct tttcatcttt attcctccaa aatcatttct atgaacgtag
     4081 tataacagaa gaggtctgaa aaacaatctg gtatgtcaca ttaactagta aatttgtata
     4141 aatagtaaaa ttttggttga catagcgatt tttattgcat aaaattcact tgaatacaaa
     4201 aactaatatg ttagtttagg aggggaaaaa tgattgattt aaacaactgg aggaacttta
     4261 tccctgaatt atctcaaaaa gaagtagaaa tacctcattt tgaccgtgca tcattaagag
     4321 aaaatggaca aaaaaatcct gtctggatcc atttcggtgg cggaaatctt tatcgaggat
     4381 ttcatgcaga aatcgctcaa cgtttagcgg atctaggcga attgtccact ggtgtcattg
     4441 tttgtgaaac attcgatgaa gaaataattg aaagggctta ccaagatttt gatgatttga
     4501 ttcttgaagt ggtcatgcca agcagtggag agttcgtcaa acgtatatta gctgcgacag
     4561 ctgacagtct gtattgtcat ccagattgtt ctgaacactt tgataagatg attcgacact
     4621 ttgaaaatcc tagtttacaa ttagttactt ttgctattac tgaaaaaggt tatcgattga
     4681 aagatacaaa taataaatat ataggaccga ttgcagtcga tataaaaaat ggtccaaaga
     4741 agccatcaca tacaatgagt attcttgctg tgctacttta taaacgtttt ttatctggag
     4801 cttggccgat tgcacttgtc tctacagata atttttcaca aaacggtagt caattacgta
     4861 cttctttgct aacgatcgca aaagaatggc aaaaggaagg ttatgtacaa aatacattta
     4921 tccaatactt atcgaatgaa gaaattgttg cgtttccatg gacgatgatt gatcgtatca
     4981 ccccgaatcc ttccttgtca gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt
     5041 cgattttcca aaccgcaaaa ggaaataata ttgctccatt tgtcaataca gaggacatcc
     5101 attatttaat catggaagat cattttccca atggacgtcc gaaattcgaa aaagctgggg
     5161 tattcttgac tgatcgtgca acggtagata aagcagatac gatgaaggtg acgacttgcc
     5221 ttaatccttt gcatacgtct ttagctatta tgggttgttt actggggtat aggagcatag
     5281 ctgaggagat gaaagatcca gatttatcgc tgctagtttc tgaaatcggg tacaatgaag
     5341 gcctcccagt tgtggagtct ccaggtatca ttgatccaaa gatctttctg gaagaagtaa
     5401 ttactgtgcg cttttctaat ccaaatattc ctaatatgcc tcagcgaatc gccacggata
     5461 cctctcaaaa ggtggcgatt cgttatggtg agacgattaa aaaatatatg gaaatagaag
     5521 ttaaagaccc gcagacactt gtattcatac cattgactct cgctgcttgg cttcgatacc
     5581 ttattggcat tgatgatcaa ggaaaaccat tcatcccgag tccagaccca ctattgacag
     5641 accttcagca taagctttcc gatattcaac tgggtacgca agatacatca catattcatg
     5701 agaaaatggc tcctattttg tcaaataaag aaatttttgg tgtagatctt taccaagcag
     5761 agcttggcag aaaaatagaa gaaatgttca tcaagatggt agcaggacca aatgccgtac
     5821 gaaaaacatt acaggattac ttacaagaac atggaggaaa tttatcatgg aaatgacttt
     5881 tagatggtac ggatcacagg aagaaaagat taccttagat gaaatcagac agattcctgc
     5941 ggtcaaaggg attgtaggca cattgatgga tataccagta ggagaggtat ggcctaggga
     6001 gaggatcaaa gaactaaaag aagaaattga gtctcgtggg ttaacactaa aagtaattga
     6061 aagcgtcaat gtgcatgatg ccattaaaat tggattacct tcccgtgagc agtatatcga
     6121 aaactataag cagacaatcc gaaacttggc agaatatgga atagaagtca tctgctataa
     6181 ttttatgcct gtatttgact gggtgaaatc tgaccttgac taccgcttag aagacggctc
     6241 atccacgtta gcttttatat ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg
     6301 catcgaacat tccagcaatg aatttggatt gccgggatgg gaacctgagc gcttagctcc
     6361 tatcaaatcc ttgcttgaag cctatgcatc agtagacgaa gaaaaacttc gagaaaattt
     6421 tgcctatttt ctccaatcaa tcatcccaac atgtgaagag gtaggggtca aaatggctgt
     6481 acatccagat gatccgccat actcgatttt tggattagct cgaatcgtaa agaatcgaga
     6541 agatttagat tggttgtgtc gcatagtaga cagcccttat aacggaatca ctctttgtac
     6601 agggagtatt gcagaagatc cagaaaatga tgtatatggg attttagcag aattttgcca
     6661 aagagatcga atcccatttg cacatgttcg aaatattaaa tttttagcta atcgagattt
     6721 ctatgaggca ccacataaat ccgaatacgg ttcattagac atgtaccgca tactaaaaac
     6781 aatgtatgac aacggattta acggatacat ccgtcctgat catggaagaa tgatctggaa
     6841 tgagacagga agaccaggat acggcttata tgatcgagca ttaggtgctg tttatctaaa
     6901 tggattatgg gaagcaatcg aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact
     6961 gtaattttat tatgtaaata agaaggatgt tcaaattctt gatacataaa tccggaggtt
     7021 gttaataagc attcggattt atgtatctta tttggtctag gaagtaactt gattttcttt
     7081 tttcgattga tccatgagtg cttcttcttt taattcaaag atatattctg agatcctcat
     7141 aatcactttt ttccagtctt ctggtgtgat gattcctttt aaaatataga tttttttgat
     7201 atgagaaggc aattgtttca aagggcagtc gctgataacg atgttgcagt tctccataaa
     7261 atcattttgt attttgatcg attcatttga aaaaatttgt tctatcttaa cgatcagatc
     7321 tttactgata taaaaatcac gcgtatagtt cacaccaatc acaattcgcg gctgtttttt
     7381 gtagcgatca tacaaatgta ctaggtcttc aaccatccat tgcacatttt ccttttctaa
     7441 aataactggt aacaagtgtt gattttgttc tctgaaatcc atataaaatt gttcaatggc
     7501 ggtttggact tctttcaata cgctatgttc ttgataaagc atgaaagtag gaaggggcgt
     7561 atttggttga ttgaagttaa gaaatccatt ataaagttta tttcgtgcga atgccaatga
     7621 gtaaagaatc ttttcttttt caggtataaa caaatcaaat acagtacaaa gggactccac
     7681 catttctctt gaataaagaa catgaggaac gtttgctttt tttaaaagtt tgtattgctc
     7741 caagcttgtt tcttccgatt cgattttcgt gatagaaagt cgagctaaaa tattcactaa
     7801 gatgcgttgt tcgcgacaaa ttggcagatt aggtcgcaaa atatctacag agagatcaat
     7861 aaaaaagtca atatattcat actctgtgaa ttctttttgt acgtctttca cagaagttgc
     7921 aggaaaattc tctttacata tttttaatag aaaatataat ttatctatca taccgctatt
     7981 tagtcggtca agtacttctt ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg
     8041 atcttcatca taaaattctg tagcagtact tgaaaagatg ttccaataaa tatctaatgc
     8101 tgcatattga atttgtacct cttttccaga aaaagacttt ctgccattat tagaaaagga
     8161 gatagacaaa ccgtactatg ctaaaaatgc attaatcttt ttcattttcg tatacaaata
     8221 agaggcacta tagtttgttt gctgactgaa actgataatc gaatttgtct gacagctgaa
     8281 caaagaagtc aagatctgat aactgactga ttcgttgcag tatcttccaa atagacgata
     8341 atagcagtaa gccggatcaa ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc
     8401 aatatgcagt tctgttagat tgagacgctc aaagtcttgt tctaattctt tgatgtatag
     8461 taaaaaattt gattcagaca tatggaaatg atctgctaaa tattgtgtag taataaagaa
     8521 acgatgttca gataggaaaa gaaaaatatc taattttgta ttgctttttt tcttcaagat
     8581 aaacgtttta atcgcttctt gagtttgcat gaaagaggct cctttttact taaattgtta
     8641 aaacttgaaa atattactgt ttttttcata aatttagaca aaaaaacagt aaacatatag
     8701 gtagaatctc tactatctta tttcttgcat acaaaaaaat caaggagtga gagagttatg
     8761 agatattcaa ccatcttaat atcaacattt gcagccttaa gcttatcgct tactataaat
     8821 cagacagtcc acacagagga gacagaaaca gccaaatgga cagaaaccaa gtgggccaac
     8881 gtcaatcgaa ttacctttca ggatgaattt tcatccttca attcaggagt cgcaaaatta
     8941 agaaaaggac aaacagcgac catcaaagca aaaattgact attcgggaga cttagcacct
     9001 atactacaat cagctgttac atttgagaat gttgatccca ctttgtcgat tggctatgac
     9061 gagaacactt tgactatcca acacaagcaa gcgatattta ccttcaccgt tacattaaat
     9121 caggacttgt taaaaccagc gctttttact gtcaaggtat cagatggact accgaacgat
     9181 catcatttaa cgccgtatag tcagcggcag acaattgagc aagattcagc catcgactca
     9241 ggaggagatc ttgttgaaga accaacagat aaaccagaaa atgaaaataa gccagaagta
     9301 ccgcctacag aaaatccaga cggagaacag aaaccggaaa tagaacctgg agaagaaccc
     9361 gatacagaaa ctcaaccaga gccagacaat aaatcaaaac ctgaaataac ccctggagaa
     9421 aaaccagatg taactccaga accagatacg gattctggaa accaaacagt accggaaaca
     9481 aatccagaca cagataatga aactgaaaat ccagaaaaac cagaggtaga tccagaagaa
     9541 aaaccagatg tgactccaga accagatacg gacgcaagag accaaggaat accagaaaaa
     9601 ataaacaaaa aaacaattca agaggatgga aaaaaagaga gtaaaaaatc aaatctagca
     9661 attctaaaaa taaatgaaga gcagctgaat aaaaaatcac gaatatttga tagcgcccaa
     9721 tcagctgaaa ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa acgaaaaata
     9781 aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc ggaatcattc
     9841 tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga tctctctaac
     9901 atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag aaaatgaccc
     9961 caaaatacca gattgaaatc tgatatttgg ggtcgttcat actttatctt tcttcatatt
    10021 tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc actttcaata
    10081 catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc
    10141 gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca
    10201 tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc gtaatcagga
    10261 tataaacaac atctcggatg cctccttggg aataagctat gaaagataga ggaatacaag
    10321 aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc gctaaactca
    10381 gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgta tttaaaagag
    10441 cgacaacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta tcggcaaaat
    10501 agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt gagaatacaa
    10561 ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata gcaaacgcca
    10621 ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct
    10681 gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca ggatctgcat
    10741 cttttggctg atgctcgtaa aaattaataa cggactggat catgtcaaat gtttgatgaa
    10801 taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc agtgtataaa
    10861 tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt tgaacaagat
    10921 gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata cttctaacta
    10981 agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt tcattcgcca
    11041 aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata aacacgttat
    11101 tcattatatg atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc taaatgacgt
    11161 tatcttatga gttggttttc ctcataaaat cgttcatcta gcagtttcag ccctttttct
    11221 ggtcttgatg aaaattatct agcatttttc gggtcagatg ttgcagtgta tccaattctt
    11281 cctgttcaag tggaagataa gacttgatct tttgtggaat accttctgct tgtttttgca
    11341 acgcagcacc ttgagcagtc agcgtaacat ttacgattct ttcatcaccc gtttcacgct
    11401 gacgattaat ataatttttt tctgctaact tttttagtaa gggagtcagc gtaccagaat
    11461 ccagacacaa atcatttcct agctctttca ctgaagtctt tttcttttcc cataaaacta
    11521 acatcacaag atactgggta taggtaagat tcagcggctt gaggagggga gtgtagtgtc
    11581 taatgatttc tttagaggcg gcatataaag gaaaacatac ttgattaact aaatatagat
    11641 cttccatttt tccacctccg ctgttttact atagtgtatc atagcggatt tacacaaaaa
    11701 agtctattca ctaactgctt tcagagtaac ttctatattt ccagctgtag cttttgaata
    11761 cggacacact ttatgcgctg cctctaataa ttcttgcgtt tcttccagag aaagaccttc
    11821 gatagatcct tcaatctcta cacctaagac aaagctcgtt tcactttctg aataaaggga
    11881 aacttgtgca ctgacagtag aagccccaga gatatttttt tgctgtttga tcaattctaa
    11941 tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt tggttgcatt
    12001 ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat gtacgatcgg gattatgcac
    12061 ttctccagag cgtccgcctg tgttgatcat tttcgtagaa tacatttttt tcataaagtt
    12121 accaccttta attatatttt atacaattga attttacaca atataattgt ggaaagcaaa
    12181 gaaatggatt ggcataagat aaataagaga aagcacatga aacagacgcg ctttctcgaa
    12241 tagcaaacat atacaaaaaa tatcagagac aaaaaataat ctcatcataa acattacata
    12301 tatttctcaa aaagttgttt tctttcttca aaaattactt cacgatggac ctgatgaagc
    12361 atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa gtccatcatt
    12421 tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg tgtgtcctcg
    12481 atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt aggagcagtc
    12541 aaaccaagag acgtgttttc atccagcata tggaatctcg taccatacaa ggtaaaaatg
    12601 acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc atcaaagatt
    12661 gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatcgat ttccgttcct
    12721 ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct tttattatac
    12781 cagctaaaga aaacgcattc aaaaaaatag cggatttact taccaactca ttcgattgga
    12841 tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac aaaggcactt
    12901 ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt cacaacgatc
    12961 aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag ggtaaggcca
    13021 cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc actcatgacc
    13081 ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat tgaagtgatt
    13141 gcaatagata aaatagcata aagaaactct tctattccaa aaataaacag attaagagaa
    13201 acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa atatttttta
    13261 aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata taaaatcgca
    13321 actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc tgaagtaatc
    13381 attgtttctg gaactattgc aatgattaaa ggaaaaacca agcttccgaa aagtacttta
    13441 aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat attcaacact
    13501 aaaataacta tagctcggtc aaagcctaaa gcagcttcca gtaatattcc tagaccgctg
    13561 acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac tcctaatatg
    13621 aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc tttcatttat
    13681 tcacttcctt aattcattaa taatactctt atcttattat aacttacttt tcaccttaag
    13741 ttaactttaa ttaataaaaa aattaggcta gtctaaatta ttatgtttca ttcttctaga
    13801 atatacttct acgccaaaaa ctgttaaaaa acttttactt tagaaaattg gagaaattga
    13861 tcgcttaaga taaattcagg acactaaata gtacaggaag tagactaaaa cttttaaaag
    13921 ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa aaaactgtta
    13981 ccgataacat ttttgaaagc gttgtctttt gcttctctat cacttatagt gatgacgtca
    14041 tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg accggaaagt
    14101 gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac taggaaaaat
    14161 gatatcaaaa aagtatatgt gctatgtgga gatccatata ccataacaac tgaaaaatgg
    14221 tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga ttattgggaa
    14281 attgaagtga cgagcgaaac aagtcgctta cagtatgcgt tccatgttgt tggggaagat
    14341 aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa tgtgattact
    14401 gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt cactattcca
    14461 gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc aaacggggac
    14521 aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga ccgagaagac
    14581 tttttcgggg gagatttgca aggtgtgatg gatcatttgg attacttgac ggaccttggc
    14641 atcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa atacgatacg
    14701 attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa aaacttgatt
    14761 gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa tcatatgggt
    14821 gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat ctataaagac
    14881 tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt accagaaact
    14941 gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa actaaataca
    15001 gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat aaaagaattc
    15061 gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa
    15121 ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga gatttggcat
    15181 tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta tgcatttaca
    15241 gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga
    15301 atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt caacttatta
    15361 gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga attgatgaag
    15421 tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta cgggacagaa
    15481 atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg ggagaaggag
    15541 gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag gaaacagttg
    15601 tctaagatta tttctgaagg ttctactcag tggctaatag taaatgatcg tgaagataag
    15661 ctctatttca cacggaaact agagggacag atcatctatg tttactttaa tcagtcaaaa
    15721 gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg tcagttgtta
    15781 gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg aaaactgtta
    15841 ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa taagggtgta
    15901 acagcacgtt gaatacaaag gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg
    15961 gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc aatggtaact
    16021 cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt gattctggat
    16081 ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca
    16141 agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat gggcctgctg
    16201 gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta ggacagcaag
    16261 gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca gacaaagccc
    16321 aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc cttgttttgt
    16381 actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg gagaatctat
    16441 ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga tttttagcta
    16501 aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc tatgtatttg
    16561 gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa
    16621 tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa
    16681 gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca atcattgacg
    16741 gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta gccaaaattc
    16801 ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga tgggtagtca
    16861 gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta acgaatcagg
    16921 aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg
    16981 aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa tatgaaacag
    17041 ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga
    17101 tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa
    17161 caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc atttttataa
    17221 aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat tatcttgctc
    17281 ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg gcattcagat
    17341 gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt cacttattcc
    17401 agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt tcgccatatt
    17461 cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag gattggttac
    17521 tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag gcactttgca
    17581 attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag acgcttatcg
    17641 agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg aagtcataca
    17701 taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc ttctcatgat
    17761 ttttacgatc attttcccag tcctagttac attgttcact gcatttacta attatgactt
    17821 caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact tttttagtat
    17881 cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg
    17941 gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca
    18001 ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac catgggcggt
    18061 cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg taggtgccat
    18121 caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca gtgcaatctc
    18181 ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg gctggttagg
    18241 atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag aagacttata
    18301 tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc
    18361 tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga atttcaataa
    18421 tttctccatg atctatctgt ttaacaatgg cggtcccgga agcgtaggcg gaggagctgg
    18481 ttctactgat attcttattt cctggatcta taaacttacg actggaactt ctccgcagta
    18541 ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg tgtcattgct
    18601 ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa atttcattcg
    18661 agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct tttaatactg
    18721 tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa gtcaggaaat
    18781 attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt tactcggcta
    18841 ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc tgtcctaaca
    18901 atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg ctatcgtttt
    18961 atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc gactatggca
    19021 gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa gtactggttc
    19081 ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat gaaaggatac
    19141 tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc agggcatttc
    19201 aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt tcaagcatta
    19261 tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct tcgaacacct
    19321 gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac aaaccaaaga
    19381 gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct attattcttc
    19441 tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg ataggagcag
    19501 agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc aactcaagtg
    19561 tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt atttttagtt
    19621 tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatctaagc gattccatta
    19681 gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa attttctgaa
    19741 cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt gacccctgaa
    19801 attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac ttttattgat
    19861 tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg ccgctatacg
    19921 atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga tttccaacga
    19981 tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc ttatagttta
    20041 agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc ttcctttttc
    20101 ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga gagtgcaaat
    20161 ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg gtttatccac
    20221 tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct gcttttatgg
    20281 gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta agcgatttat
    20341 gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt tctgaaaaaa
    20401 ttaaacttct tagttgattc ttttcttttt tagtattaac gtaatagggt agttttttta
    20461 aggaggaaaa tcatgttaga gaaaacttta tacaatcaat tattttcacg ttctttctcg
    20521 ctccctgtcg aagtaaatta ttgggacggt acaacgaaac aatacggaga tacggacact
    20581 cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat tacaagtaac
    20641 gcatctttgg cactaggaga agcctatatg gatcatcgaa tcgagatcga gggaagtatc
    20701 caagcattga tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg aaaagattat
    20761 ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga agatatccat
    20821 gcgcattatg acttaggtaa cgacttttat aaactttggc tagatccaac tttgacttat
    20881 tcttgtgctt atttcaatac acctgaagat acgttggaac aagcacaaat caataaagtc
    20941 catcatatct tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga tatcggttgt
    21001 ggctggggaa cattgatgtt cactgctacc aaagagtata atgtcaaggc aactgggatc
    21061 acactaagtg aagaacaata cgatttgatt accaaaagaa taaaagaaga acatttagaa
    21121 gataaatgcc gtgtattatt aatggattac cgcgaactaa aaggcgaaac gtttgatcat
    21181 atcacaagtg taggtatgtt cgaacatgta ggttctgaaa atcttgaagg ttatttcaaa
    21241 gtggtaaaag atttattgaa accaaaagga acagctttga tccatggaat cagccgacaa
    21301 caaggcggtg cgacaaatgc ttggatcaat aaatatattt tccctggcgg ttatgtccct
    21361 ggtgttactg aattagttga taatattaca aaaaacgatt tgcaactgat tgatttggaa
    21421 agcttacgtc gtgactacca attgacatta gaacattgga caaagaattt tcatcaagtc
    21481 caagatcaag tcacgaaaga aatgggtgat tccttctatc gaatgtggga tctatattta
    21541 caagcttgtg cagcttcatt tgaatcaagt aatatcgatg tgattcaata tttgcttgtt
    21601 caacctggaa acaatgacat cccaatgcat cgtgcataat tgaggcaaaa gtactgttta
    21661 aagactattt aaaaagaagc taagttttat gatgctccag aagtgaactt ttttgtctaa
    21721 cttctaggat tgtctctaaa gcttagtttc tttttcttta tattttcata ataaaagttt
    21781 ggttctttaa tttttaaaca tgacagaaaa ttttttcttc gttatacttg taatagaata
    21841 ctattttata acaaagggga tcgaatcaat gaaaaaaact gttatttata ctagtttatt
    21901 tgcagctttg tttttagctg cttgtcaatc aaataccaat caaggcacat ctgggaatac
    21961 gtcttccagt agcgaacagc gctcttcaca ggtatcggac tcctcaacaa aagcaagcac
    22021 atcttcgtct agccagacaa aaagttctag tgaagcaagt actcagtcag atatcaaagt
    22081 gtctgcagct cgtgcgatcg aattgttcca agataaatac ccagatactg caattactag
    22141 tttggaatta gatagtgatt ggggatctta cttctacaag atagaaggtg tcgatgatca
    22201 aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa atggagacag agaatccaga
    22261 aactctagat cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc tggatgttga
    22321 taaattgatc acgattgagg aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac
    22381 ggcaacagat tgggatttgg ataaagattt aggtactact tattgggaag tcaaagtaaa
    22441 agacggcaat caaagtacat cagtcaagat caatagccaa acaggcgaag tactgtcaac
    22501 tgaacaagat gattaagaat ttcttcggaa aacaagagtt gatttttctt ttagtcatga
    22561 tacactagac aaaataactt gtttgaagga gcgatttact tgagtttaga aacgaccttt
    22621 gccaatcata tctttgccaa tcctttgatg aatgcatctg gtgtccactg catgactacc
    22681 caggaattag atgaactggc tcattcagaa gcaggagcat ttatcactaa gagctgtacg
    22741 atcaatgaac gaaaaggaaa tcccgaacca cgctattttg atgttccttt agggagtatc
    22801 aattctatgg gacttccaaa cttaggtttt tcttattatc tagagtatgc tcttgcgtat
    22861 gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt ccattgccgg gatgagtgtc
    22921 caggaaaact tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa
    22981 ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac tagcttacga ttttgaggcc
    23041 acttatgaaa cactgaaaga agtattcagc atcttttcta agccgctagg aatcaaattg
    23101 cctccttatt ttgattttgc tcattttgat cagatggctg atatcttgaa tcagtttcca
    23161 ttgacctatg tcaatgctat caacagtgtg ggcaatggtt tgtatatcga tacagaacaa
    23221 gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca ttggcggaga gtatatcaag
    23281 ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc
    23341 attggtacag gtgggattcg gacaggacaa gatgcatttg aacatctgct atgtggcgca
    23401 agcatgttac agattggtac cgagcttcat aaagaagggc cggaaatttt ttctagaatc
    23461 ataaaagaat taacgcagat tatgtcagaa aaaggatata cgtcaattga cgaatttaaa
    23521 ggcaaattac gcaccatttc atagcaaaga gacgaaggtc atgattgatc ttcgtctttt
    23581 tttataaaaa acactcgtct gttcattcct gtgcaagcgt actcattgta tgttagacga
    23641 acttgtggga aaataacaga ggcaaaaagg aggataatat gaatgatgca acagaattag
    23701 caaaagagct taaagaaaaa gaatttagtt ttcaagaact agttacggat atacagaaaa
    23761 aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta
    23821 attcccacga gctattgatt ccccagtcag aaaaagggcc attggctggt atcccatttc
    23881 ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc tacttcagga tctcgtttat
    23941 ttgaaacaca ccgagcttct catacatcta attatgtgcg gcaagcagaa gcgataggtc
    24001 tagttccttt tggacaaacc aatgcgccag agtttggttt caaaaatatt acagacccag
    24061 tcatctatgg cccagcaaga aacccttgga accttgaatg tacaccgggc ggatcaagtg
    24121 gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt agctggtgct agtgatggcg
    24181 gtggttctat tagaataccc gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag
    24241 ggacgatgcc agttggtcca tatgcttgga gaggatggca aggagctgct attgatttcg
    24301 gcttgacagt ttctatgaga gacacggagg ctttatttta tgggatgaga agtatccatt
    24361 ccggtgcgcc atatcaagta tcgccagttg agtggcaaac acatccaaga aaaaaacggt
    24421 tgaagatagc tttttgtgtt gattcaccga tccactcaca gattagtgaa gaggcagtac
    24481 ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg gcatgatttg acagagattc
    24541 cttacccact agatggtcgt aaactgatca cgtcttatta tcagatgaat ggcgcagaaa
    24601 cagcagcaat gatccgctcg attgaaacag gcatccagcg agctgtcaga aaagaagaac
    24661 ttgaacccat ttcttggact ttgttccaat atggtcagaa gctctctgcc gctacttaca
    24721 tcgattctct gcatgtatgg gatgaagcag caattgtcat ggaaaaccta catcaagaat
    24781 acgatctatt cctttcacca acgactgcat ttaccgcccc taaaatcagc gaagatttgc
    24841 aaagtgatgc gataagagca cagatggaac acatcagtga ttgttctgaa gtagaaggtt
    24901 tagacgttgt ttcttccatg tttgaggaga gcttgaagat cacaccttat acacagttgg
    24961 ctaatctgac tggacaacca gcaatcagct tgcctactca tttgacaaaa gaaaacatgc
    25021 ctctaggaat ccaatttatg gctgccagag gaagagaaga tttgctattt caaatcggct
    25081 atctatttga agcaaatcat cagttccatt taccaaatcc atggaaatag cagaaacaag
    25141 gagaagaaga atgaagaaaa aaagattgtt tgctctaata actagtgcct tttttatttt
    25201 aagcgcttgc ggtacagctg ccgctaatca ggaagataac agctggagta atgtagaaaa
    25261 aaagaaagaa ttagtagtag cgacttctgg cacactgttt cccgcatctt attataatga
    25321 tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa gaggtggcta aacggttaaa
    25381 tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa atcacttctg tttcaaaaag
    25441 agaatcagat tttgctgcga acgattttgg tatcacaaaa gaaagagaaa aaaaattctc
    25501 cctttcagag actttcaaat attcatttga cagtatgatc gtacgaaaaa aagatgattc
    25561 aggaatccac tcacttgaag atcttaaagg gaaaaaagca gctggcgaac ccaatactag
    25621 ctatatgcgt ttagcggaaa aacacggagc taaattagtc acatatgaca atgccacgaa
    25681 tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat gttattctaa atgattatta
    25741 tctgcagaaa atggccgtat ctgctttacc agatatccct gtaaaaatac tggagaacgt
    25801 ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa
    25861 gaaaatcgat ggcgtattaa aagagatgaa agaagatggg acgacaaaga aaatatctga
    25921 aaaatatttt caaactgatg tttcagtcaa accgaaagaa acgatggctg agtcggtgag
    25981 tgagtgatgt actttcctct aattgatttt tccctcatgt ggacttcact tcctttcgta
    26041 ctgcaaggct tggtatacac attagggatt ggcttcgtgt cgtttctttt aggaaatctg
    26101 gtagggttgc ttttgacagt tttaggtctt ttggattggc tgcctctgaa tgtatttatt
    26161 cgtttttatt tatcgttctt tagaggcatt cccgcactag tcctgctgtt tctgttatat
    26221 tttgggttac cttatcaatt aagtgcctta acggcttcag tcatctgctt tacgattacc
    26281 agcagtgcat ttattggtga aatctatcga ggatcactag caggcgtttc ttcaggtcag
    26341 tgggacgctg gtttagcttc tggtttttct ttctttgggg tattgaaata tatcgttttg
    26401 ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg tagctatgga tctagtgaaa
    26461 ggtacttcgt tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc aaagatcata
    26521 ggcggtcgga cattcgatta tttttctctc tatattttag ttgcactgat ttattgggtc
    26581 atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa aacgttctat gcatcagtca
    26641 atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat ttctattttt tgtttcttgt
    26701 atattctgcc tagtatttta aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg
    26761 aggtagaaaa catgaagacc ccgctaacca tcgaaataga atcctcgtta taccaatatt
    26821 gcatcgagca aggcggcttg gttgtagaag aagtcacgat gcctgatgac caaggaattg
    26881 tagatacgct tagctgcttg acaaaatttg atggaagccg agaatggcga tgctatgagc
    26941 tgaaagtgac aaaagcagat tttcgctcta cagccaaact gtcatttgtt ggacattata
    27001 attattttgt tttaccaagt aaactatatg aagaagtaaa aactgaaatc cctacaggaa
    27061 taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc tctggtacat
    27121 ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt accggaagaa gctttgacaa
    27181 accgcttcat ggcttcttta ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg
    27241 taaaatattt tccaaaagat caattgtaca aagaattgaa aagacgttca gaaaatcaag
    27301 atccattttc taacgaacga tattatgaac ggtttttaga ggaaatagct agtcaggcaa
    27361 tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac
    27421 aacagcagat ccgtcggttg cctacagagc cattggagtg aaaaatatgt actatcctta
    27481 tttaagagga aaacaatttg acctattagc attaaaagaa gcactgtcgc gaggattgct
    27541 ctccaataaa atccaaccag tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt
    27601 gatcgaatta tttcaaaaaa agcaccatcc aatcgctgtg atccaaaatc cccaagtagg
    27661 acaattcaaa ttattcgatc agcatgtgca cagttggaca gtcaaagaaa atagctctgt
    27721 cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa gtactggatt cacctccagc
    27781 gtttcttgtt ttcgatggac agcactaccc aaaagataca gaggtgtgga aacagttggc
    27841 aggagttgat tcgaaatttt tgataccaga tactagtcgc tttcgcatct ggctgcccga
    27901 aaacaaaatt gtgatccgtg actcttttca aacgagaaag catgttgaaa gttatgcaga
    27961 taaaacagat gactttttta gtgatgacta tctttttttc cacgcagacg gatatattgg
    28021 attttctgat ttcacgatcg aaggtagccg ttatttcgat aaagggttcc ctagccgagc
    28081 gcttgctatc catctaacgt atattgatgc ttacggcaat atacgcgtga aacattttgt
    28141 ctctgacagt aacgactctg ctaaagacca agcattgaag tttcttgaag caggagataa
    28201 aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg attacttcag gaatgattga
    28261 attattggct ctttaccagc aacaaaaatt ccctggactg ggagtgttga aaaagtggtc
    28321 acttatgcat catttggaac ttattagtca cctgctagat catccaaaag attggcttaa
    28381 atattattca aaagttcagg aactatatga agtgatccaa tgattagata atgtaaatga
    28441 aaaaggaata gaaaagagga aactggatgt atattcggca aacaaggaaa gaagaagtag
    28501 aagaagtggt agaaatcatt aatgccgcag cggatttcat tgcttcgcaa ggctctcctc
    28561 aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag
    28621 aaagctatgt attaatagtg gaagatcaaa ttgctggaac agctgcatta gtctcaggcg
    28681 tggatcccgt ttacacaaaa atagatggta gttggaaaga atcaactggt ccttatttat
    28741 cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca aaaccgttct
    28801 tagaacattt gttgacgatt gcagccagca aaaatatcca agatatccgt atcgatactt
    28861 atccagctaa tgaaccgatg gaaaagacca tctattctgc tggattcact tatcaaggga
    28921 tgattgaatt tccttttgaa cacggcgaaa gaaaagcata tcaaataatc atcaattgac
    28981 tataaaatca tctgagcaaa gataaagaca ttaacactac tgaaaaaaac aatcatatga
    29041 tagccaagag gttgtaacag aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa
    29101 tgcttctcaa atttttgtgg aattttagcg tattttacga aatagctgct ttctgaaact
    29161 cccttcatcg agaggtcgtg acatagcttt tgtcacgatc tctcggctta tcctacgagt
    29221 attattcgat acatttatat caataatcct ttttttattt tatctgcagc ttcttggaac
    29281 gctccatcgt ttgacacgac taaaagtgag gggatagctt tcaaacgttt caactgtgct
    29341 aaatcttcta cataaaccgc cagtctggct gaaatctgtt cagatgaatc tccacgtttt
    29401 ttcaggcgcg attgcaatgt ttctttagag atcgtaataa agataggacg gactaccgtc
    29461 ccgaaatact catataaatg ccaaaaacca tttgctgtca aaacggcgta acaatttcct
    29521 actgtcagtt tctttttaat ttctgcttta ctggaaccat atttatgttg agcatattgg
    29581 tcccactctg caaactcatt tcccttgatc atctgattga atgtattgtc agaaacaaaa
    29641 taataatcct cttgatcttt ttccccaggt cttggtgatc gattcgtgta agtaatgatc
    29701 ttttgttccg gctgaaaaat agcttctgcg agtgaagttt ttccagaacc agaaggaccg
    29761 ataaatacat agcaataatt cattgtcatt tctcctttga tgattgaaca agtgaaaaag
    29821 ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc ttttttgtat tttataccag
    29881 caagccagtc acaatagcac ttaaaaagct gactaaagtc gctgttagca aaattttcaa
    29941 tccgtgtttt gcgacaagat ttcctttttc ttcatttaat cccttcatcg caccagaaat
    30001 gatcccgata gaagagaagt tggcaaatga aacgaggaag acagatataa ttgctgttgt
    30061 acgctcagta aaatggaaat taccatttgc taattctgtc atagcaacaa attcatttgt
    30121 aaccagttta gtcgccatga tactaccggc atccactgct tctttccatg ggattccgct
    30181 gatgaaggca agaggggcaa agatgtaacc taggatctct tggaaagtga tgccgaatat
    30241 cccgtggaag atagcattga tcatggcaat caaagcaaca aaaccgataa gcatagcagc
    30301 aaccgtgatc gccacatgga aaccatcaag aatgtattct cccaacattt ggaaaaatgt
    30361 ctgttctttg ttttcttcaa tgatcagctc atcttctttt tcttctaata catagggatt
    30421 tacaattgaa gccagaatat aaccgccaaa taagttcaaa acaagggcag tgatcacata
    30481 tttagaatcg atcagtgcca tataagatcc gacgatcgac atagaaactg tagacatagc
    30541 cgatacactc atggtgaaca aacgtttctc agataggaag ggtaattcct tttttatgga
    30601 aataaaaact tccgattgtc ctaaaatagc agaagctact ccattataag attccaattt
    30661 gcccatcccg ttgattttac ttaatcctag accaacagct ttcataaata atggaagaat
    30721 cttgatatag cgtagaatac cgataatagc agaaataaat acaattggca tcaatacgga
    30781 aagaaagaaa ggtgcacttc ctttatttgc tacaccggca aaaacaaagt caacaccttg
    30841 cccagcaaaa gcaagaagat gatcaaacat ttttgccaat acagaaacaa ccgctgtacc
    30901 aaaagttgta cgtaataata cgaaaccaag aacaagctgt aaaacgaaca tcacaattag
    30961 tggtttcaat ttgattgctt ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac
    31021 aaataaacca ataataccta ataaataact caaataatca ctcctattcc aaattccgtt
    31081 ttattttacc tgttttgcaa ataaaatact aatctttagt gaaattttca ttactttttt
    31141 agagtgaaac gcattcattt tccattagaa aataaagcga acaaaataaa aggtttaaaa
    31201 agtgtaacgt cttagtaaag tctataaaaa aacgaaacta tcgattaagc taatagtttc
    31261 gttttcaaca tttataagta aactttttct attggtaata tagattttcg gaagggtaga
    31321 caggtgcgca ggtaatatcg attcctagtt ttttcaatgt ttgttcatca tttttattga
    31381 ccatcacagt agagtgggct tgtgctccct caagttcagc tagtttatca taagcaactt
    31441 gagcgattgg attcgttact gcactgattg ataaagcgat caagacttca ttgatgctta
    31501 gtgcatccaa agaggaatga agtgtctttg atttcaatcc ttgtattgtt tcaagaacta
    31561 atggtgacaa taaaggaatg tcatctgcga tatgggctaa atgttttact gcatttaaga
    31621 taactgccgc tgacgcatcc atcaatgaac ttgtacgccc ggtaatgatg acttcatctg
    31681 gtaattcgat tgcaatcaca gcagaaggat cttttgtagg actttgcgct tgaatcttat
    31741 ctgcatagtc atgggcaggg actaccacgt ttcggtcttc aggacgtaaa ccgacttctt
    31801 ccataatcag tttgattcga ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc
    31861 attctgtttg gaaataacga cggatgattt cttgtttaga ggcttcttgg acaacggtat
    31921 catccacgat cccaaatcct acacgattca cacccatgtc agtaggagat tgataaacag
    31981 attctttacc agtaattttt tcaataatac gtttgattac aggaaatgtc tcaacatccc
    32041 gattatagtt cacagctact ttattgtagg catcaaagtg gaaggaatcg atcatattga
    32101 catcttttag atcgactgtg gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa
    32161 cattccatac tgggaaagtt tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt
    32221 catgatacag ttggttcaag caagtagcga gctttccact tcctgggcca ggagctgtca
    32281 ctacaacgat tggtttagtt gtttcaatat actcattttt tccaaaacca tcttctgaga
    32341 cgattttttc tacgttgact ggatagtctt cgatttctga gtgtttatag accttgatgt
    32401 ttcgtctttc tagtttattg ataaagactt tcgtggcagg ctgtccacta tagcgagtga
    32461 taacgacact gttgattaat aagccataac cacgtaattc atcgattaac cgcaagatat
    32521 ccatatcata agtaatccca taatcaccac ggatcttatt acgttcaata tctccagcat
    32581 agacgcaaat cagtatttca gcttgatctt tcaatttttg cagcaatttg attttcgcat
    32641 cttcttcaaa tcctggaagt acacgtttcg catgcttgtc gtcaactaac ttcccaccaa
    32701 attctaaata taatttgtca tagtggttca ctcgttccaa aatatacgct gactgctctt
    32761 caatatattt ttctgaatca aaaccaattt ttttcactag gttccctcct taagtactaa
    32821 agcatttttt catttttttg aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac
    32881 aaagaaaatg gtttaaacca acttgtccag atagttattc attggtatga tagtttttga
    32941 taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa acgcatataa acactatatc
    33001 cgttcgattt ttaccggtta tgaaagaatg cttgaagatc ccgaattgga acaattgacg
    33061 tttacttatc taaacgaaga aacgcagcta actcgtgatg agcatcagcg cattcatttc
    33121 acaacaagag atctgcctag taaataaaaa ataagataaa ggtcatcgga ttcgattctg
    33181 tcctatcaga tggaaagact agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc
    33241 ctgttttatg gagcattgcc cgctcttttt tataacaatt ttttacagaa aatggtacta
    33301 ttgaagtatt aataagtgaa aaacagatga gtaagaggag gcaagtagta tgaaagagcc
    33361 agatacaaat gcaaattatg agccagacga actagaggag acgaacgcgg atcaggatac
    33421 aggacaagaa tcgtcttctt tttacgatat caaagaaaaa gcaagcatga atctttccca
    33481 attattagaa gacttacagg cttttgaaaa agctgtccgc gaagaagata tccctgagat
    33541 ctaccgttta tacaatggcc gattgaatga agaattaaaa agaagctcaa atgaaaatca
    33601 cgagatcgat caattgctga tgcgtaagat ccatgatcgt tttttacagg aatttccttt
    33661 tatggaacaa gtagaaaaca tctcgccaac gatgagctat tacaaaatcg gcacttatta
    33721 ccatgatcgg cccacggtcg gtattgatgc aagtctgcca gaaatttttg ttttgcctaa
    33781 gatcgatgaa gagtgggaga aatattccca accggagtca acggactatg acaaaaaaat
    33841 caatgagcta gacgcaaagg tgatcactgc tcagacagaa attgaacgtt tagatgaaca
    33901 aataaaagaa atcaatcgac aaatgactga tttgaacgac aataaaggat tcttgaatcg
    33961 taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag aagcaagtac tatctaatga
    34021 aaaattaggc tggcttcctt atattgagac gcctgaaaca atccagcagc aaaaagaagc
    34081 attgaagcaa gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag
    34141 gcaaatcaga cgctatttcg gtagcaaaga gggattcggt caagcaatcc atgaattctt
    34201 gatgaattat cttggcaatg agaatcctaa taatcaatct aatgaaggag gttccgagta
    34261 tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga acaaacagaa gatcatgaag
    34321 aaacgaacac ttcattggaa gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc
    34381 ctatgctaga ggaaacaaaa atacatgaaa aagaaaaagt aactccgacg aatacgaata
    34441 ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag
    34501 agcaagatca tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt
    34561 tacgtatgag aaaagatttg atcgaatcag attttccaca attattagaa tcatatgatc
    34621 gttttatatt gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg atcgaatact
    34681 tcacgtatga attactgaaa ccgttgaatg atattggtct ggaacggtca aattcagctt
    34741 atgacacatg gaaaacaaga catttttcga tcaattatac gttgaatgga caaaacgaat
    34801 tagtcttttc ctttgtatta ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc
    34861 tcctaaacgt atcaccagag acaatggaaa tcaatgtcca agatgaccgg gtgctttctt
    34921 tgatccgaca ctggtcagtg gatcgagtat tctctgcagg gcagatcacg atattcaatc
    34981 acaaattgaa tcaattatta gcccatgcaa gaacacttgg ttttaccgtt aatcagacat
    35041 tactagacaa tacgaagcca cttcttttga atcttcagag cgaatttgaa ttgacgaatg
    35101 aagtattgga tgatattttt atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga
    35161 tcaacgaaac tacttatcaa gtacgattag ataaaggaca gtctttaacg ataacaacaa
    35221 acgaaaaaga tcaaacagtc ttgtcgatct catctggaga ctacccacga tctgtcattg
    35281 atttctttat caattacgaa ttcttggtcc cattaatggt gagaaagtcc tgattggata
    35341 agttggatta ccagatgtca ttcagataat tctgttccaa aggacttgcg gcagttatcc
    35401 agaataatt
//
LOCUS       DACESX010000013        34791 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.013, whole genome shotgun sequence.
ACCESSION   DACESX010000013 DACESX010000000
VERSION     DACESX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.013"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(443..2335)
                     /locus_tag="IXF88_000557"
     CDS             complement(443..2335)
                     /locus_tag="IXF88_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507307.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2332..4059)
                     /locus_tag="IXF88_000558"
     CDS             complement(2332..4059)
                     /locus_tag="IXF88_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507308.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4075..4725)
                     /locus_tag="IXF88_000559"
     CDS             complement(4075..4725)
                     /locus_tag="IXF88_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1507309.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4929..5225
                     /locus_tag="IXF88_000560"
     CDS             4929..5225
                     /locus_tag="IXF88_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1507310.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5417..6037
                     /locus_tag="IXF88_000561"
     CDS             5417..6037
                     /locus_tag="IXF88_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1507311.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6129..7295)
                     /locus_tag="IXF88_000562"
     CDS             complement(6129..7295)
                     /locus_tag="IXF88_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1507312.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7307..7660)
                     /locus_tag="IXF88_000563"
     CDS             complement(7307..7660)
                     /locus_tag="IXF88_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1507313.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7788..8810
                     /locus_tag="IXF88_000564"
     CDS             7788..8810
                     /locus_tag="IXF88_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1507314.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9249..9683)
                     /locus_tag="IXF88_000565"
     CDS             complement(9249..9683)
                     /locus_tag="IXF88_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507315.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9793..11652)
                     /locus_tag="IXF88_000566"
     CDS             complement(9793..11652)
                     /locus_tag="IXF88_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1507316.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11770..12930)
                     /locus_tag="IXF88_000567"
     CDS             complement(11770..12930)
                     /locus_tag="IXF88_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ1507317.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13237..14661)
                     /gene="araA"
                     /locus_tag="IXF88_000568"
     CDS             complement(13237..14661)
                     /gene="araA"
                     /locus_tag="IXF88_000568"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1507318.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14677..15375)
                     /locus_tag="IXF88_000569"
     CDS             complement(14677..15375)
                     /locus_tag="IXF88_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1507319.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15332..16993)
                     /locus_tag="IXF88_000570"
     CDS             complement(15332..16993)
                     /locus_tag="IXF88_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1507320.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17211..18611
                     /locus_tag="IXF88_000571"
     CDS             17211..18611
                     /locus_tag="IXF88_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1507321.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18863..19942)
                     /locus_tag="IXF88_000572"
     CDS             complement(18863..19942)
                     /locus_tag="IXF88_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1507322.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20028..20987)
                     /locus_tag="IXF88_000573"
     CDS             complement(20028..20987)
                     /locus_tag="IXF88_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1507323.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20999..22522)
                     /locus_tag="IXF88_000574"
     CDS             complement(20999..22522)
                     /locus_tag="IXF88_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1507324.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22532..23095)
                     /locus_tag="IXF88_000575"
     CDS             complement(22532..23095)
                     /locus_tag="IXF88_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1507325.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23101..23964)
                     /locus_tag="IXF88_000576"
     CDS             complement(23101..23964)
                     /locus_tag="IXF88_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1507326.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23978..24874)
                     /locus_tag="IXF88_000577"
     CDS             complement(23978..24874)
                     /locus_tag="IXF88_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1507327.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVEAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25049..26320)
                     /locus_tag="IXF88_000578"
     CDS             complement(25049..26320)
                     /locus_tag="IXF88_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1507328.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26310..27827)
                     /locus_tag="IXF88_000579"
     CDS             complement(26310..27827)
                     /locus_tag="IXF88_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1507329.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGSWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            28025..28915
                     /locus_tag="IXF88_000580"
     CDS             28025..28915
                     /locus_tag="IXF88_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1507330.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28988..29812)
                     /gene="nadE"
                     /locus_tag="IXF88_000581"
     CDS             complement(28988..29812)
                     /gene="nadE"
                     /locus_tag="IXF88_000581"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1507331.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29824..31296)
                     /locus_tag="IXF88_000582"
     CDS             complement(29824..31296)
                     /locus_tag="IXF88_000582"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1507332.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31933..32295)
                     /locus_tag="IXF88_000583"
     CDS             complement(31933..32295)
                     /locus_tag="IXF88_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1507333.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32518..33270
                     /locus_tag="IXF88_000584"
     CDS             32518..33270
                     /locus_tag="IXF88_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1507334.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33410..34333)
                     /locus_tag="IXF88_000585"
     CDS             complement(33410..34333)
                     /locus_tag="IXF88_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1507335.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34348..34620)
                     /locus_tag="IXF88_000586"
     CDS             complement(34348..34620)
                     /locus_tag="IXF88_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507336.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10272 a         7229 c         6006 g        11284 t
ORIGIN      
        1 cgtttaagga agaggttgtg aaaaagtcgc ttagtttcga gtagtaagaa aaattttgtg
       61 aaaatggctt tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
      121 caccgtttac caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc
      181 aaaaaacagc ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag
      241 aacaccgttt aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa
      301 aacggaaaaa tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga
      361 ttgaacaccg tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa
      421 aaaataataa atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa
      481 gatctgcata gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg
      541 aaccatgatt catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa
      601 caacgaaact tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg
      661 ttcgtgtatc tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga
      721 atgcccgcgc aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt
      781 ttaatacagt gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt
      841 tagcagcctc aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat
      901 agatacttcc tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt
      961 catcacgtga aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat
     1021 cgtagaaccg ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa
     1081 tagcgaccat ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg
     1141 gtgtgtagcc gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg
     1201 gaaggccaga tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg
     1261 ctgaagcaac tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag
     1321 agaactgtgt tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg
     1381 caacttttac tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca
     1441 tcaacggcat gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct
     1501 gattttcttt ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg
     1561 tatgaccacc atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat
     1621 ctgcgaaaaa cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga
     1681 tcatcaataa cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc
     1741 caatcaatgt gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga
     1801 tgttatccat atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat
     1861 aggaaatcgg tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc
     1921 gttgggaaac acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat
     1981 acatcacgat tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg
     2041 ttacttgttg gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg
     2101 tggttgcttt ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta
     2161 gtacaaatac agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta
     2221 ccgttcccca gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac
     2281 catgtcctgg accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa
     2341 gtcctcctct cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag
     2401 ttcttcatgt gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc
     2461 atccattact gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt
     2521 tttcaatgct tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc
     2581 aaacacatag atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg
     2641 tccaccagag aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc
     2701 tgaaacaaaa tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc
     2761 atccggctta ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg
     2821 aggaacaaag cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc
     2881 atttatttgg atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt
     2941 cgttttacct gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa
     3001 atcaatatct tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa
     3061 ggccaatgtt gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat
     3121 accgattttt tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac
     3181 catgataaaa atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat
     3241 aaatgcaatc agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca
     3301 agtgattgct acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac
     3361 gatcgtatta actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt
     3421 ttcttcaaag cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg
     3481 aaataccagg tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa
     3541 gtacatgatt ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt
     3601 caactgatga tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt
     3661 gatcatcata cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt
     3721 tcttgtgatc aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga
     3781 ttttttatag acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa
     3841 aaatgtattt gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc
     3901 tgtatgccaa atataatcga catctccttg cgctacccct ttatcaataa tattggaggt
     3961 aagtgtcggc aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat
     4021 tgctgaagtc aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt
     4081 atatactccg tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac
     4141 tgagtaatct tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt
     4201 attcatcact aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt
     4261 ccgatcgatc aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg
     4321 aggcgttttt tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt
     4381 cataaacaag ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt
     4441 agaaaaatag gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa
     4501 catatcttta gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt
     4561 atcttcaaaa tactgataga aactgcctct agggatatcc gctaatttga cgatattagc
     4621 aatcgaagct tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct
     4681 tttttgtttt tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc
     4741 tttcaagatg acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg
     4801 caagacattt gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga
     4861 atccttccat ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga
     4921 tgagataaat ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg
     4981 acagcatcgt tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact
     5041 cgctttctat ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac
     5101 tttattatat tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg
     5161 ctaatggttt tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg
     5221 cttgaatatt gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct
     5281 tttataccct gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc
     5341 cttttctttt tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa
     5401 aggagaaggt aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga
     5461 agaatcgcgc acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc
     5521 agatgatgaa gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga
     5581 attattatca ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag
     5641 tcaacaacaa aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa
     5701 aatcgttatc gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt
     5761 cgataccgtc aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt
     5821 agtgacaggt aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga
     5881 ctttgcctct caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca
     5941 gctattcatc gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc
     6001 attagataaa gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa
     6061 agagacagag gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc
     6121 tctttttgtt attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt
     6181 gtgtaccatt caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc
     6241 aagatcacac cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt
     6301 actggatgga ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt
     6361 gtctgctcta ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg
     6421 gctaaaaaga tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa
     6481 aaacccgcaa taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc
     6541 ataaacatga ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact
     6601 tgactattca tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat
     6661 ttgacaaaat aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc
     6721 atcgtcagca gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga
     6781 actgtactct tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct
     6841 gctgtttcca actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat
     6901 ttattatcaa tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc
     6961 tgaatcttag ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac
     7021 agcccaatga tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga
     7081 ttcatcaagt aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat
     7141 tgccagattg gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt
     7201 aacaatacaa caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac
     7261 gataccggtc tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa
     7321 tttggcttcc caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa
     7381 tttgtccttc ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat
     7441 tcccattggc catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg
     7501 ttccgcctgc tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt
     7561 ttcgtaaggc agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta
     7621 ttgcttctgt cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg
     7681 ctttcaaaaa cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag
     7741 aataaaaacg aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga
     7801 taattttagg aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata
     7861 cagaaaaaga agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg
     7921 tagcaaaaag taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg
     7981 ccggtgcgta cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt
     8041 tgtgttatgt agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag
     8101 gtgaagtaaa tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat
     8161 tccatcaaga agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg
     8221 atctgactcc tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt
     8281 atgatgtgtc cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa
     8341 caggccctcg ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat
     8401 tagacagcac agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac
     8461 agaaaatatc gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca
     8521 tttcaaatga tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat
     8581 tcgcagtttt tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg
     8641 attttccccg cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga
     8701 acaccgataa tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga
     8761 aaaaagatgt ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg
     8821 agaatgaaat gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt
     8881 tctttgatgg gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag
     8941 ccgatttaca gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc
     9001 atactatctg aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta
     9061 caaataaaaa accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg
     9121 agtaagtaca aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac
     9181 tctatcataa aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa
     9241 gatttctttt attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg
     9301 tctgtcgctt ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat
     9361 tcaagatcga tactttggat catttcttca cagcctgcta catatgaaag ataagctttt
     9421 tcaaaatttt tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata
     9481 agcattgccc gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc
     9541 gatttgtcca gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc
     9601 ggatgcattc cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca
     9661 taatagccaa tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt
     9721 attctacttg tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac
     9781 agctcgccat tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc
     9841 gacaatgtca tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa
     9901 ttgcgcttct ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag
     9961 ttcctgttct aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc
    10021 cattagattc gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc
    10081 taattctcct ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt
    10141 ttttgcttgg atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa
    10201 agtccttcta gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata
    10261 ggaaatatct tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc
    10321 aggatagtct agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa
    10381 atgaatgaac tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga
    10441 ctcatcagaa atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct
    10501 gatgaactgg tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt
    10561 gtatggatgg atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg
    10621 aagctgatcc atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa
    10681 cgccatagaa aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt
    10741 ttgagaaagc atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg
    10801 ctcaccagat acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac
    10861 gaacacgatg ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg
    10921 gtatttttca gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg
    10981 ttctaccttc agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt
    11041 ctggctgtaa ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg
    11101 attgttgaaa ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg
    11161 gatcaattgg aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc
    11221 tatcacggtt cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt
    11281 cagcaatccg atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata
    11341 actcattttc atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga
    11401 acctaaaggg acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag
    11461 acaggctaag aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac
    11521 ggatagatca ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat
    11581 actgatgatt gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat
    11641 atatcttttc aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat
    11701 tttagttgta cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat
    11761 tctctttctt tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat
    11821 tttttctagc tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat
    11881 caatatgtag acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt
    11941 ctcattcagc agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt
    12001 gaatcgaaac ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac
    12061 cgtaccgaac aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa
    12121 agcttcctga gtcgatcccc ctagatgagg caacataagt atacggtcat tttctaagaa
    12181 ctcttcttct ggaaaatcag tcaagtattt agccagatgt tgtttttcca atgcttgcat
    12241 aacagcatat ttatcaacaa tttcacttct tcctacattg ataagtacag catctttttt
    12301 cattttttct atattcgcct gatcgatcaa tccttttgta tcttctgtca acggcagcat
    12361 gactacgatg taatccgagg tagaaagaac tgtttctaaa tctgcttgcc gaacataatc
    12421 caactgggca tcgcggatag aataacccaa gacatccatc cctaagctat agcaacttag
    12481 cgctactttt gtgcctatcg ctccaagacc aagaagaccg atggttttgc cttgcaattc
    12541 tcgtcccaca taagcacttc ttttgttttc tgcctgttct aaaatattag gacctgttag
    12601 tgtttgtacc atacgacttg cttcgattat cggacgggaa gataagagca aacagcacaa
    12661 aacaagctct tttactgcat tagcatttac acctggtgta ttcatgacga tcgtcccgtt
    12721 ttcagaagct ttttcaacgt tgatcgtgtt caccccaaca ccagctcttg aaattgctag
    12781 caaatctggt gtaagccatt catctttcac tttcgtacta cgaataacta ttccttcggc
    12841 tggcaaatca tctattattt tgaaatattc cccttgtaac ttttctatct cttgttctgg
    12901 aatagcatca atcaaatgaa cgtgatacat aaaaccccct ccttactaac aagtatctcg
    12961 aaatttctgc aattagacaa ctgtttacca tttgacttcc tagaagcaaa aagagcagtt
    13021 agagcagagt aggctgagtg ataaaaataa ggctatgaca agttactgcc ataagcctta
    13081 ttttcgagag caaaatattg agggtgagat atcttgataa atggtgttcc gttagctgac
    13141 attgataata agactaaatt tcaaaaaagt tgaaaagcaa atttcgtcaa tctcttctta
    13201 tttcttaaaa ctaagtggct ttttcacaac ctctttttat ctaataacca ctgtttccaa
    13261 ctcaactaat tttgcccacg tttcaatttg atctgttgtt agatttagcg agacaacggt
    13321 atgatgaccg ccacctgcct gaatccatga atggacacct tcatggaaat tcggtttagt
    13381 tttccataac acacgtgcaa caggtaaatt aggtgcttca gtcgttggtt caaaggcttc
    13441 tacttcattg atcagcagtt tataatgcgt accgaaatct gccatcgata cgactacccc
    13501 ttctcctgct ttcccatcaa atacaagacg cgccggatct tctctatctc ccattgataa
    13561 aggagaaaca acgattcttg gtttcgtcgc agcaagcgtc ggatctactt ccatcatatg
    13621 ggattccaag attgcttctt ggccactagc tagctcgtaa gtataatctt ccatgaatcc
    13681 tgtatctttc cctcgagcca tgattttcag caaccgatca atcgctgcag ttttccagtc
    13741 gccttcccca gcaaaaccgt atccttctgc catcaaacgt tgtacagcta gtccaggtag
    13801 ttgtttcatt ccatacaggt cttcaaaatt agtcgtaaaa gcgttatagc ctccttcttc
    13861 taagaaacgg cgaataccga tttcctgctg agcttgtact tttacatgtt cctcccatgt
    13921 cttttcttca taatcaccat agtcgaaggt atacaattcc ttgtactctt caaataattg
    13981 atcgacttct tcgtctgata cgcgatcgat tacttgtaca agatctccga taccaaaata
    14041 atcaacagtc caaccaaatt gaatctgtgc ttccactttg tctccttctg tgacacccac
    14101 gtttctcatg ttgtcaccaa aacgagccac ttttattcca aaactttcat tataggctac
    14161 tgcaacatcc atccaatcag caatctcttt ttggatctca ggtcgtttcc agtagccaac
    14221 gacgatttta ttttgtttat ttaatcgagc attgatgaac ccatattcgc ggtcaccatg
    14281 agcggattga tttaaattca tgaaatccat atcgatcgtt ttccaaggaa tgctttcgtt
    14341 atattgcgta gctaaatgaa gcaatggttt ttgtagaagc tttgtcccgc ggatccacat
    14401 ttttgctggt gagaaagtat gcatccaagt gatcacacct gctacttctt ctcgataatt
    14461 gacctctttc atgatttttg tgatcgtatc agcagtaact gctaattctt gaaggcggat
    14521 tggataaggc aattggccat tttcattcag ttcatcgacc atttcttgtg catgtttctt
    14581 tacttctcgc agtgcttctt ccccatataa atgctgtgat cctacaacaa accaaaattc
    14641 tttttctcca atattcaaca taattagcca ctccttttat ttgttttgtc cgtaatacgc
    14701 attttttccg tgttttcgta aataatgttt atctaaaatc cgttgaggca gctcttctga
    14761 aaatgaattg agctgtctgg taaataaatt catcttgcat acttcatcta gtacgactgc
    14821 gttcattacc gcactttgcg catctttccc ccaagtaaat ggaccgtgtc cgtgtaaaag
    14881 tacccctgga attgctaagg gatcaatttt gcgttctttg aatgtttcta tgataacttt
    14941 tcctgtttca tattcgtacc cgctgtcgat ttcctgttgt gtcaaaaatc gagcacaagg
    15001 taccgatcca taaaaagtat ctgcatgcgt cgtccccatc gcaggaacat ccaatcctgc
    15061 ttgtgcccag atcgtcgccc atgtcgagtg agtatggacg attcctccga tttgaggaaa
    15121 tgaacgatat aaaacagcat gagtaggcgt atccgaagag ggattgagtt taccttcaat
    15181 gatatgattg tccaagtcca caacgaccat gtcgtctgct gtcatctctt cataactgac
    15241 tccgctaggt ttgataacga aatatcgtgt ttcaggatca aaagcgctca catttcccca
    15301 tgtatatttg acgagtccgt gtttaggcaa gtcaagattt gcttggtata cttcttcttt
    15361 cagttgttcc agcattttct atcactcctt tttcttgcaa ttgatgaatc gctgtttttt
    15421 caatgtcgat cccctcacgg taacgtctca taaatatctg atagcctgct atttcttctg
    15481 ctgaaggtgt gatggtgatt ccttggtctt gtccaaacac gtcttctgcc aagaagtttt
    15541 ctaaagactt cccatgtcga tccatcatat aggctgctaa taacgcgatt ccccacgctc
    15601 caccttctcc ggctgtgtcc atcactgtaa ctggtgcctc catagcagat gctagaatcc
    15661 gctgtccaac ttctggtgtt ttaaaaatcc ccccatgccc cactaaacga tcaatttgga
    15721 cattctcaga tttcaatata tccattccga ttctcattgc accaaaagca ctggctaaat
    15781 ggatccgcat gaaattcgct aaatcaaaac ggctacctgg tgtacgtaca aataatggcc
    15841 ttccttcatt tactcctgta atgttttcac cggaaaaata accataagaa agtaatcccc
    15901 cgcaatccgt atcgccttct agcgctttgt tgaataatac cgtgaataat ttatctctgc
    15961 ttatctccat acccaaagaa ttggtaaatt cttcaaaaat actgacccaa gcattgatat
    16021 ccgaagagca gttattcgta tgaaccatgc ctactagttc tcctgaaggt gtagtgacca
    16081 tgtcgatttc cggatggaca ttttctagtt ctttttctaa tacgatcata gcgaaggcgg
    16141 aagtaccagc cgaaatgttc cctgtccctt tggctacact gttcgtagcg accataccgg
    16201 ttcctgcatc cccttctggc ggacaaaccg ggattcctgg tctcaaattt cccgtagggt
    16261 cgatcagata agcccctgat tcgcttaact tgccagcttc ttctcctgct actagaactt
    16321 taggcaaaat gtgatccaag tcaatcgcta cgtgcgcttt ttcgattaac tgaccaaaaa
    16381 tatttttcat ttcttgatgg taggttttcg ttgaaggatc gataggaaac atgcctgatg
    16441 catctcctac gcctagtact ttctcaccag taagctgcca atggacataa cctgcaagcg
    16501 ttgtcaaaaa attaatttct ttcacatgtt cttctttatt caaaatagat tgatacaaat
    16561 gagcgatact ccatctctgc ggaatattgt attgaaattt ttccgttaat attcgttctg
    16621 cttcttcagt gattgtattt cgccaagtac gaaatggtac tagtaactgt tccttgctat
    16681 caaatgctag ataaccgtgc atcattgcac tgaacccaat cgacccgatt gtcgtaagat
    16741 tctcgccata gatttcgaat acttctgctg ccatttttcg gtaactgacg cgcagtcctt
    16801 tccaaatatc ttctaatgaa taggtccaga taccattttc tagtttgttt tcccactcat
    16861 aactaccagc ggcaatagga gtaaaagatg aatcaatcaa tacagctttg atcctagttg
    16921 atccaaactc aatccctaaa gatgtacgac catctatgac atctaacata ctttgcgcat
    16981 ttacttcctt cataatgatt taactccctc cttctcgtca aacctgatca aaccgtcttt
    17041 ttagtagcgc ttacaatgat tattgtatac ttttgtacgt acatgtcaag cagttattta
    17101 caagaataca ataaaaaaca cttgttttgt acgtacataa gttataaaat gaaattgctt
    17161 acaaaatctg atcaaccgcc ttctcgaaat tctagaagga gagaatactt atgaaaaaag
    17221 aaaaaaaagt ttcctcatcg tttatttatt tttttggcgc atttggcggc attctatttg
    17281 gatatgatat tggtgttatg accggcgcct tacctttttt acagcatgat tggggtttgg
    17341 caggtaaagc ttctttgata ggatggatca cgtcttcggt catgttaggt gcgatccttg
    17401 gcggctcttt atctggtctt ctttctgata agcttggacg acgaaaaatg attcttcttt
    17461 ctgcacttat ttttatggct ggttctgtcc tttctgcatc tgcccctcac aatggttctt
    17521 attttctgat tgcagcaagg attcttctag gtttagctgt tggcgctgca tctgccctag
    17581 ttcctgctta tatgtctgaa atggctcctg ctagactaag gggaagattg tcagggatca
    17641 atcaagtgat gattgcttct ggcatgctcc tttcctatgt tgcagattat ttgctaaaag
    17701 ggttgcctga aacgatggct tggcgcgtga tgttagggct cgctgctgtc cccgcgctga
    17761 ttttattttt cggtgtttta gctctaccag agtcaccgcg ttttcttatg caatctggcc
    17821 gcttggaaga ggcaaaaaga gtattgaact atattcggac accaaaagaa gctgaacagg
    17881 aattcgaaca aatccagctg aatgtcaaac aagaaaagac aaccggaact tcttggcata
    17941 ctttgttttt agaaaaatat cgttccttag tcattgccgg tattggtgta gcagtattcc
    18001 agcaatttca aggagctaat gctatttttt actatattcc gctgatcgta gaaaaagcga
    18061 ctgggcatgc agcgagcgat gcattgatgt ggccgatcat ccaaggaatc attttagttg
    18121 ctggttcttt gctctttttg gttattgcag acaagttcaa tcggcgaact ttattgaaaa
    18181 taggcggtag cgtgatggga ctttctttca tcctaccagc tgttttagga actgtactgg
    18241 atgctcatac aaattcgttg ttgattctct tgtttttatg tatttatgtc gcattttatt
    18301 cttgcacatg ggctccactg acatgggtaa tcgtaggcga aatattccca ctagctgtcc
    18361 gcgggcgagc atctggccta gcctcttctt ttaattggat tggttctttc ctagttggct
    18421 tgctttttcc tgtgatgact gcttctatgt ctcaagaaat cgttttcggc atttttggga
    18481 tcatctgctt tttaggggtt ttatttattc aagagatcgt accagaaact cgtgggaaat
    18541 ctttagagga aatcgaacaa agtgcatcta aaaaaactta tcctaaacgt atttctatcc
    18601 atcattctta aagaggttgt gacagaagtg gtcagcttca agaaataaga aggaaccgct
    18661 tagtttccag tcacaaggaa caatttcaaa aaacagcttc tcatgtttga tgaatattgt
    18721 gacttgtgac cagttggtag gagaacaccg tttagtggag aggtggtgaa agaaacgttc
    18781 agccttgagc gatgacaaaa gctctcacaa acattgcgac gagtaaccgt aggagcaata
    18841 agaggcggta aaaacccgtc tcttattttt cgttttcttt cactgtttcc cgttcaacta
    18901 gtacaggttc atagagaata tttggctgtg cctgtccttt ttcaatcgtt gtgacgatcc
    18961 accgtgcggc atcttgtccc aacttttctt ttggatgcgt caatgttgtc aattttttat
    19021 tccctaaatg gcttaaagaa gaatcgtctg ttcctacgat cgataggtca tctggaatat
    19081 gatagccatt ttctgtcaac ttatctagca ttaaactagc tatttgatcg ttatagcaaa
    19141 tgatgccatc taaagcttgt tcttctttca accgttgtac tgttttttca gcgattgttt
    19201 ctcgtgtttc cgtggtgaaa gtaatgatat cttccgtcaa aaaacgcaca ttatactgtt
    19261 cacacgcgct gataaaacct ttcatcctgt attttccttg aagatcgtcg atttttgtga
    19321 tgaacaacag acgctgatgt ccttttttta tcaaatattc tgctgccata aaaccgactt
    19381 tcacatcatc taaacaaata taagaggcat ttaactcttc ataagcagca ttgatcatca
    19441 ccatcggaat gccttgttct ctcaaagcaa cataaagtgc taagtttggg ttatactgat
    19501 tacttttcgt aggctcaacg atcaaaccat ctaccccata agcaatcatt ttcttcaaac
    19561 actctttctc caattcgtga tcgttattcg tactggctaa aagcaatgaa tacccagatt
    19621 ctcgtaattc attttcgatt cctcggataa tcgacggaaa aatataatca gagagatatg
    19681 tggtgatgac accgatcgtt tttgttctgt tcaatggtgt tttttctttc ttatagctgt
    19741 catctacata tgtgcccgac cctttttctt tacgcagata gccctcattt accaacacag
    19801 cgattgcttg ccgaatcgta tgtctgctga cgtcatatat ttcttgcagc ttgaattctg
    19861 agggaatggt tgtatttact tcgtattcct cacgaaggat cttttgtttt atctcttctg
    19921 caatatcttg atattttgcc atttgccttg ccccttttta tttgtacgta catatatcat
    19981 aacgagtctg caaggtttat tcaagcaaaa atcaaggttt cttgtgctca taaggcatac
    20041 ggaacaggtt caccaaattc tggaaatcct tgctcattcc acgtaaagca ttgcgcgttc
    20101 gcgtgccgat taggattgtc taacggatcc cctttttggt tcttctctgg tcgagcatga
    20161 tagatcaaaa tatcaaagca cccgtcttct gaaaccgtaa acgagttgtg accaggacca
    20221 aattgcttat ttttttcaga agaaacgaat actggctccg ataatttatg ccatgattct
    20281 tttaataaaa gattcttgtg ctcatcagcc cacagcaggc ccatggcata gttttcatct
    20341 gttgcactac cagaatacgt aatgaatatt ttcccattgc gagtaaggac tgctggacct
    20401 tcatttactg aaaaaccccg tttttcccat tcatactcag gtatagaaag gaggatctgc
    20461 ttccccttta acgtccaagg attatccatt tcggatagat aaagattaga attcccagta
    20521 atttcaggat cttgttgggc ccacacataa tatagctttc cttgagaaga gaaagttgtt
    20581 ccgtctaaac tgaaagtttc ccttttcgtt tttatttgtc ccttttctat ccaatctgta
    20641 ttcattggat ctatatgctg attttcaagt acgaacatgc ggtgatgatg agattcatca
    20701 cgtatagttt tatgattgct tgcagcaaag taaatatacc attttccatt gatataatgg
    20761 acttcaggtg cccagatcaa ttcactcatc ggtccttctt catgagcctg ccatacaatc
    20821 aggcttgcag catgttctaa ttcattcagt gactttgccc ttcttaattc aatcacttgg
    20881 tagcctggaa ccgatcctgt aaaataataa taaccatctg tatgtttata tacccaagga
    20941 tctgcccgtt gccgaacgat aggattatta taaagtgtct tcatcataac aaatttcctc
    21001 acattttctt tattctaatt acattccaag agtatttttc tatccctaca tttaattcgt
    21061 catgtttttc tattgaaaaa tagtttattt caggttttat tttttctggt tcttcacgtg
    21121 tatttttgtc caacagatcg ctactggtta atgtgtagtg ctctccttga tctagcaaat
    21181 tgaaatcatg ttcaatcgtt atcttttgtt gttcatttgt gcgattgatc aagaaaatag
    21241 tcagctgatt gccgttatct acgatcacac tgtccacata aggaacattt tttatctttt
    21301 cacaatcata aacagggcaa atgatttgtt gtttcaatac ttgaccttta gcatatttcg
    21361 ataagtgttg catcacataa taagttggat tcctccatgc ttgtccttta gcatctgtca
    21421 gtattaaagg tattgtgttt actaacaatg attgacaact aatctttacg cgatctgcgt
    21481 gtcgtaaaat agccagccct aatgaaccaa ataataatgt atcttccata gtaaagtaac
    21541 accaatcgat gggactaccg acttcaaaat ccctatgtct tttttccggt tgggcatgta
    21601 cattccattc atcaaacgct agatacattg ttttctcact tctcaaatat ccttttacat
    21661 aatcacaagt agcaacgatt tcttcaattt gacgatcgaa tcgataagct tttgcaaggt
    21721 atgtcgctgt atcatcacgc tcatttttag gaggtttagt caagtcttct tcatcatact
    21781 tatcaatata gttatgcaaa gctaaataat ctacattctc ataagcttct tctaatgcga
    21841 tacgatccca ttcagggtac gatggtaaac gtggcgtaga gcttccaact aagattgttt
    21901 caattgaatc atccactaac ttcatgactt ttgaggcttc atttgctaac ctaccatatt
    21961 cataagcact cttagccccc acttgccatg gaccgtctag ctcattccct aaacaccata
    22021 atttaacgcc aaaaggcttt ttttgcccat tttttattct taaatcactc cagaaggttc
    22081 cttttggaaa attacaatat tctactaaat tttttgcttc atcaattcct tttgttccta
    22141 aattcaccgt gaatatcggt ttcgcttgta catgactgat ccatttaaaa aattcgtgga
    22201 gtccaaaaga atttggttct atacctcgcc atgctaaatc aatttttttt ggtcgttgtt
    22261 ctttcctacc tacagtatcc acccaatcat aaccagatgt aaagtttcct ccaggatagc
    22321 gaatgacacc taattctaat tcctttacat aagagaggac atcttgtcta aacccgtcac
    22381 catcagctgt ttcatggccc ggttcataaa tccctgtata gacgacactt cccatatgct
    22441 caacaaatga gccaaacagc cgagaactga tctctcctac cgtaaaatcc tctctgattt
    22501 ttactttcac ttcttcgtcc aacagctttc cctacctttc atttcctata gtcaagataa
    22561 tagatacaag tttccatacg ccaccagcaa agataaaaat aaaaggaggg aaaagaaata
    22621 tgagcagtac aaatcctaat aacaatacgc aacaaagaat atttctcaaa aaatcaaaaa
    22681 atactttcca tgcactttgt tcaaaaattt gtaaaaatgt gatatctttg ttttcagaaa
    22741 tcacatatat ccaggataaa agaaacatgg aataaataag taaaactaca tatccactaa
    22801 ttgaaagtac gattgtctgt tcttctaata attgattaac aagaaaataa ataatgatcg
    22861 ttaacagact aaggaacaag ttcttaccaa aatgttgctt tattccttga aagaaaaaat
    22921 aaatgatatt tcctgatccc tcacctttcc attgcgccat tacataaaaa acagcacatg
    22981 tagcaggcac gatagtaata acaggcaaac acacgattac ccagagaata cctaataaga
    23041 tgtaatcaga aaccacaatc aactgtccat aaagtttagt ctcaactatt ttcatcttcc
    23101 tcactctttc attccagaca tagctgttcc ttgaacaaaa tatttctgtg caaaaatata
    23161 aaccactagt aatggcagca ttgaaataac cgttccagcc attacaggtc cataaaaaga
    23221 aatgttctgc cctgtaaata aagctaaccc tgctggtaaa gttctcattt ttgaactact
    23281 tgtcaatatc aatggataaa gcaagtcatt ccaactattc atcagcgtga aaatccctaa
    23341 tgataaaaga gtaggtttgc tcaatggcaa catgattttc aaaaaaatat taaactcact
    23401 taatccatca atgcgtgctg cctcttccaa agaatctggt aaagtaataa aaaaggaacg
    23461 catcatgaag ataccaaatg cagttgtcat acgaggaatg atcaaacctg cataagtatc
    23521 aagcatgcca agcaaattga cttcaataaa tagaggaatc atgaagactt ggaatgggat
    23581 catcattgtc agcaaaacga aatagaacaa aactgatttt cctttgaaac gcatacgagc
    23641 aaatgcatac cccgcaagag aatcaaagaa tacagaagtg atcactgtcc ctccagcaaa
    23701 aatcactgta tttttgatgt aatctaataa agggatcgtt gtccaaacct taatatagtt
    23761 atctagggta tagctatctg caaatattct aggcggaaat gagataatgt ctttttcgtg
    23821 tttaaaagaa gaaataatca gccacaaaaa aggaaacgca ataaccaaca atataactag
    23881 catactaatc gttcgaatga tttttttacc actaactgct tttcgactat tcacagtaac
    23941 tgtaatcgag ctttctggtg ccatacaggt ttccctccta tttcatttgt ttttctttcc
    24001 gtgacatgta cacattagtg gagatagcta tagttgcaac aatgataaac aaaaccgaag
    24061 acagtgctga tgcatagccc aattcataag gtgctgtaaa tcctcgactg taaatatatt
    24121 gaacagctgt ttctgtctta aattgtgggc caccagcggt tgcaacatat acttggtcaa
    24181 acacttgcat cgaaccaatc agattcgtta aaatacaaaa acctaaggag ggaacgatct
    24241 gaggtagcgt aatgttaaaa aactgctgtc gatttgttgc accatccatt tgagcagatt
    24301 catacaaaga gggagagatt ccctgaatag cagtcaagag aatcgtcata gtaacaccaa
    24361 aattcttcca aacagtcatg atggctaccg tcggcatagc aagggttgga tcgcgaaaaa
    24421 attgagggat cggcaagccg acttgtgaaa acaagtaagg aatcgcctct acattcggat
    24481 caagcaacat agaaaaaata atcgcaattg acgtcaatga acaaataact ggaatgtaaa
    24541 atgtcgaacg acaaaaacga ttgaatctgg tatttttaga taatgctact gctgctaata
    24601 aaccaacgat aatttgtgtt ggcgtttcga gtacggtgaa atataaagaa tgaatcaacg
    24661 aattaactgc tcgttcatca ctaaatatac gtgtgaaatt ttcaaaactt acaaaatttc
    24721 ttgatgtaaa gaaaatattc aaatctgtaa aactaatagc aaatgttcct aaaagcggta
    24781 tgacagtaaa taataataaa actaaaaaag atggagttaa aaacaaatac gcagcacgat
    24841 caggacgatt ccaatatttg gtgaatccgt tcatatagtc cacctcatct ttcattttga
    24901 taaaatatac aatcttggtt taaacgaatc tgaataagat atccgaacaa tcgctgtgat
    24961 ttttgcaata acacctatct gcaaatacta ggtcgatcat tcagatatct gtatcttgcc
    25021 aacagtctat cctgtataaa gttctgtact atttttgcag taattgattg atggcatcat
    25081 ctgcatcgtt catgagtgtc tcaggatctt ctcctgctaa taatttttca atcagtggat
    25141 tgatgatatc gttattgatc gcagagattt gggtaaaacc tggtaaatat ggctctgcat
    25201 agtccatttg tttcgataat tcagatacaa taggatcatt ttttacttct tcatcttttg
    25261 ctactgaatc caagtaggca ggaaaaccat tttcgatact ccatttttta ccaatttcag
    25321 ttgtattcca atatttcaaa aactcataga cagcttcttt tttattatta tctgtacttg
    25381 ccggaatggc atatcctacg ccatctaaaa tagctgtttt tttgtctgaa tcactccctt
    25441 gtggtaaggt agtcacatta taatcaatct cgtttgcttt caatccgtta tttaaccatg
    25501 gcccattgat ttcaatggct agttgaccag cattcattaa attgtccatt tcggcgccag
    25561 aaatcgattc tggaccagtc ttgtctacat ggatcatttt ttggatcgct tttaacgttt
    25621 taaggttctc aggagacgca aattcagact tagtataatc atcgttcact aatttaccgc
    25681 catttgctaa catccagtta tataacggtg cagttccatc cttattgaag acaaaacctg
    25741 atacattttt actactatca gttaattttg gtgccatttc tgtcaattct tcccaagtag
    25801 taggtggttg ttcagtatct aatccagctt gtttaaacaa atcattattc caatacatat
    25861 acatgccttg tacctgcatg gggacaaaat actgtttacc ttctacttca cctaattcaa
    25921 tagccgtttc tttaaaatca gatttatcta ctccatcata ttcccagaaa tcgtctaatg
    25981 gttgtaccgc tccgttttct acatactgtg caaaatctgg ataatttaat gcaataaagt
    26041 caggtgcttt tttagaagaa atcgctgtag gaagtttttc attcaaattg gcccaagtca
    26101 tgatatccat ttcaattttg atgtttttat catttgtttt attaaattca ttaacgattt
    26161 cttgcaatat ttcaccatca gatgcagtaa acccattcca aaaagtcaaa gtcacttctt
    26221 tatcagaatc agattctgct gaacttccgc caccgcaagc tcctaaaata cctgttgcag
    26281 ctaccaacaa agagccaatt aaccattttt tacctttcat tttttgttcc tccttcatct
    26341 gataaacgac atctgaccac attccaagat agcggtttca ataaacaatt gattttccca
    26401 tcttcagctt ttacatttgt tgaatgactt ggttttactt gttcactttt aggaagatta
    26461 gttgcttttg tatcaaatcc actcatttca gaaaaatcaa tcactccctc taatgataat
    26521 tcagacaagt gaacagttag attcatttct tcttctccac ggttaactgc aaatatgaca
    26581 agttctttgt catttattac ggctatactt tctaaatacg gcacttcttc aaaatcttcc
    26641 gtcgcatagt ttgctgaacg aatttgtggg cttaacacct ctccttgtcc atacacagca
    26701 gcttgcataa atggataata gatcgtctgt ctccaagctt ctccgtttgt ttctgtcctg
    26761 attggtgcaa taacatttac caattgagca agacaagcaa tctttactct atctgcatgt
    26821 ttcaataaag tgattaatag acagcctaat agtaatgcat cttcaaaatt atagttatct
    26881 tctagtaaag gaggagcaat ctgccaaggc gctgcttgct catcttgttc cgctgaatga
    26941 taccaaatat tccattcatc aaaggacagg ttgatctgct tcttactatg tttcttcgcc
    27001 ttgactgtat cgcacatcgc tgtcacttca gaaatgaacc gatccatgtc taaggaacgt
    27061 gcaagataat gagaaagatc atttttttgg ttgccataat attgatgcag agacaaataa
    27121 tctatttgat cgtacgcttg atcaagaact gttaactccc aatcaccaaa tgtaggcatt
    27181 tgccgattcg aacttccgca taaaaccacc tcaattgaat catctacgag tttcatcgcc
    27241 ttagccgttt cctctgctaa ccgtccatat tcttccgctg ttttatgtcc aatctgccaa
    27301 gaaccgtcca tttcattccc taaacaccat gtcttaattc caaaagggct ctcttgacca
    27361 ttcgaacgcc ttaaatcact ccaataagtt cctttaggaa aattacaata ttcgactaaa
    27421 ttacgagctg catctactcc acgagtaccc agattgacag ccatattgat ttcggccccc
    27481 acatgctgac accagttata aaattcatga atcccaactt cattcgtttc aattgtccgc
    27541 caagctaaat ccaaccttct aggtctattt gctatgggcc caatactgtc ttcccaacga
    27601 aatcctgaca caaaatttcc accaggatat ctaatcatgg gaatgttcaa ttcttttacc
    27661 aagtgaatga cgtcttggcg aaatccactt ttattagctg atggatgatc tggctgataa
    27721 atcccttcat atactgctcg tcccatatgt tcaataaatg aactgtacaa ccgcggatca
    27781 actttgccta cacgcttgac actactaatc aacattgtgg cttccataac tgacctcctt
    27841 taatttatac atgaataata cacctcgttg aaagcgttgt caataatata aatgttaaac
    27901 ttacttattt tttatcgatt ttaattaacc aaaatattaa taatgattaa aacaaataat
    27961 ttaatataac ttttattaac aaatatgtta atatataaga aaaacctttt aagaggtgat
    28021 atatatgcaa ctacaaataa acgaagaatc cttaccggta tatgaagcat tagctagtaa
    28081 aacaaggatc aagatcatcc agctactttc caaaaaaaag atgaatgtca aagatttggc
    28141 aaaagaactt ggagtgagca gtgctatcac gacgatgcac gtgaaaaagc tagaggaagc
    28201 aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt tcgagccttc gtgtcgataa
    28261 aatcgatatc agttttccag aaaaaatatt caatgctttt gatacgaaag aaacatcgat
    28321 acctattggt cactatacaa attatgcaat tgaaccaaca tgcggtttag ctacgatcca
    28381 tgattttatc gggaaagtgg atgaaccacg ctattttatg gatcctcgaa ggatggatgc
    28441 ccgcattctc tggtttacca gcggatttgt cgagtatcag gcacctaatt ttttaaatac
    28501 agaggataca ttggagatgt tggaagtttc tgtggaaatt tcttctgaat ttcctttttc
    28561 aaatgacaat tggccttcag atatcacttt cagcctaaat ggtgtggagc taggaacatg
    28621 gacgagccct ggagattttg ctgatatccg cggtaaatat acgcctgatt ggtatccaga
    28681 taatcttaat caatacggct tgctgaagac gatccgaatt accaaacact tgacgaatat
    28741 gaatggagaa cccttgtcaa acatcacgat caacgacatc cctaaagagc aagacacttg
    28801 gcatttgcga atcgaagtga aagacgatgc caaacatgtg gggggctgta ctttatttgg
    28861 taaaggattc ggtaattacg atcaggatat caaattaaaa gtttactaca gctgatattt
    28921 ttcttttcta ttttataaaa caaaaacagc caatccctat gaagggacgg ctgtttttgc
    28981 tttattgtta tttccagaaa tcatccaaaa cggaaattgg caaatgtcgt ttatgttgtg
    29041 tcttgttcca ccatgcttcg attttttctt gtgcttgagc gggaacttct tttccttcta
    29101 gataatcatc aatttcatta tatgtgacac ctagtgcgac ttcatctgca atcattggtt
    29161 tcccatcttc aagatctgca gtcggaattt ttgtgtataa tttttccggt gcattcaatg
    29221 cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa taatggcaaa atgtcggctc
    29281 caccatcacc aaatttcgtg aagaatccag taatgttttc tgcagcatga tctgtcccta
    29341 atacggctcc tgctctttct ccagctacgg catattgcgt aatcatacgt tgacgtgctt
    29401 tgatatttcc tttgttaaaa tcgctgattc ccacacctgc ttcgcttaat acttgtactt
    29461 gtgcatcaac agctggtttg atatttactc gcagactaac atcgggctgg ataaagtcaa
    29521 gagcagcttt tgcatcttct tcatctgctt gttcaccata aggaagacga acagcgataa
    29581 attgatagtt ttcatctttt gtttcttcac gcatttcttc catcgttagt tgcgccaaac
    29641 ggcctgctaa agtagaatct tggccgccgc tgatacctag tacaaaagtt ttaagaaacg
    29701 gatatttttt caaatatgct ttcataaagt caatactttt acgtatttct tcttttggat
    29761 caattgttgg tttgacacct aattcattga taatttctgt ctgcaggctc atttattctc
    29821 ctcctatgct tctgtatgaa gttctgctac gctttttttc attcgttcca tgatagccat
    29881 tttatggttc cagcattcag tagacagatc gactgggtat ttttgcggat tcaagtctcg
    29941 tttatactct tcccatagtg aatctagatt ttcttttgta tattctttga tttcgtttag
    30001 tgcaggtaat tcgtaaactc tttttccttc gataaagata tcttgtaaaa ctgggcgagc
    30061 tgtaaagtct cgaacagttt tattgataaa cgtatgcact ggatggaaca tgaagatagc
    30121 ttcttcttct cgagggtctt catcccataa tgtcacgtag tctccttcag atttcccatc
    30181 aaaatttcga gtgatgcgcc acacttgttt tttccctgga gtagttactt tttctgcatt
    30241 gcttgataac ttgatcgtat ccatcatttt tccttcatca tcttcgatcg atactagctt
    30301 gaatactgca ccaagggctg gctgatcata ggctgtgatc aattttgtcc caacacccca
    30361 tacatcaatt ttagcttttt gcatctttaa attcaaaatc gttgcttcat ccaagtcatt
    30421 cgaagcatag atttttgctt ccgtaaatcc ggctgcatct aattgttccc gaacacgctt
    30481 agatatatag gccatatctc cactgtcgat gcgtacgcct tggaagttga ttttgtcacc
    30541 taattctttc gccacgcgaa tcgcacttgg cacacctgat ttcaacgtat cataagtgtc
    30601 gactaagaac acgcagtctt tatgggtttt agcgtaggcc ataaatgctt cataatcatt
    30661 tccataagat tggactaacg aatgagcatg ggtcccgcta gctggtatcc caaatatctt
    30721 cccagcacgt acattgcttg ttgcatctgc tccgccgata tatgccgctc tagttcccca
    30781 gactgctgca tccagttctt gtgcccgtct agttccaaat tccaatagtg gatcttctcc
    30841 gatgacagat ttgatacgag ctgcttttgt cgcgatcaac gtttggaaat tgaccatatt
    30901 caacagtgct gtttctacta attggcagtg cgctaaaggc ccttccactt ggattaatgg
    30961 ttcatttgca aaaactagtt ctccttcacg agcagaacga actgttgctt taaattcaaa
    31021 attttgtaaa tactccaaaa aaccttcagg atagacttct aatccacgca agtaagcaat
    31081 atcggaatca ttgaacgtca gattctctaa ataattgact aaccgttcca atcctgcaaa
    31141 aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa cattcaaaaa cagcatgtaa
    31201 atcagctcga cctaattccc agtatgtctg catcatattg atttgatata gatccgtatg
    31261 cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg tattactcct cttggcaagt
    31321 tttcatttcc tacccattat aacaaaaaaa attaatagga aaagaaatct tccatttttt
    31381 ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa tgaactccat ctgctaaaac
    31441 tatcctttct tattggtatt taacctaaac gagaaagaat acttatttta ttcaatgtat
    31501 ccgagacacg aagaaattag cgttttctat ttttcttact tgataaaata agtagatttt
    31561 cctactacaa tcgtagccgt taagataccg atgattgaac aaatataacc aaatgtctct
    31621 acaacataat caatggcaaa agtttttgaa gcaataatac gtctttcctt ttttgcttct
    31681 tatccccaaa gaaacggata taacttatta tgtattgaat ttttttggaa aggcgaatta
    31741 tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa atatcacacg tttttaaaaa
    31801 gatacaaagt aagaaagcag ctgtcaatag aatggaaaca aaaatggcga gacatcaatc
    31861 agtctcgcct tgttttttat tgtatacatt agatatggag caggttacca taaagatgat
    31921 tattagtcgt tattaaatag aatccttcgt atttttttgt tctatcacta caatatcttt
    31981 atttggacgt ttgaattgta ttccggcacg ttctagctcc tgtttcactc ctacatggac
    32041 tttgacatct tctcgtgtca tttttaaaac gtgatgaatc gcatagtcga tttcttgcct
    32101 tgtgattttt ttcttcgtat tgatcatcac gacttgcttt ttatcatatg tgtctaccaa
    32161 tagcagttgt gttcgatcta acgaaaataa tttgtaataa ttaatagccg tattcccaaa
    32221 aaagaattcc actactttag gataaaatgt cttaatattt aaattactat tcacaggtgt
    32281 ttcgattagc ttcatctcat tcctcttttc tatatagtcg atttagatcc aatcttaagt
    32341 tatgttacca tctttcctag cattaaggaa tcggttacaa ttgaaccttt aaagacaaaa
    32401 ggatttacct tttatcactt aatagtataa cataatttcg tgagaaaaaa atacaatttt
    32461 ttcttgacca tctcgttacg taaaggatta tgcttacatt tgtcaggagg aaaaataatg
    32521 gagtacacaa taaagaaaat gtcagagata tcaggagtca gtccgagaac cttgcgcttt
    32581 tatgatgaga ttggactgct aaaaccagct aggatcaatt cttcaggtta tcggatttat
    32641 ggcaaaaaag aagtcgatcg tctgcaacac atcttatttt atcgaacaat ggcattcaag
    32701 ttggatgata tccaagaggt cttagacaat ccatcttttg accaccagaa agctttaatc
    32761 aagcatcagc aaatgttgct ggaaaaacgg gcacaaatcg ataccctctt gacaactgtc
    32821 caacagacac ttgatatgta cgaaggagga agaaaaatga gtgacaaaga aaaatttgaa
    32881 gggtttaaac aacaaaagtt gaaagaaaat gaagaatcct atggacaaga gattcgacaa
    32941 aaatacggaa aaaaaaaggt tacagcatcc aatgaaaaat ggatgaatat ggataaagaa
    33001 acgtatgaag aaatgcaaac tgttgaaaag cagatgttat ttgatctcac tctttactta
    33061 aaagaaccag aagatgaaag cttagcagac cgtatcttcc aagctcataa gaagtggttc
    33121 tcttacagct ggcctgaata tcagccaaaa gcgcataaag gactaggaat gatgtacgaa
    33181 gccgatgaac ggttcactgc atattatgac gagcgaagtg gtcatggtgc agcaaaagct
    33241 ttaaatgaaa ttatccaaaa aaaggcgtaa tcttttacat gatccaacaa aaaagtacac
    33301 gtaaaatgac ctaataacaa aacagcagac acccaagtta gatttttatg ctaacttttg
    33361 gtgtctgctc cattctttca caagtttttt catcctactt taaatatatt catcgttgaa
    33421 taaacgtata aggtattttc actttatcct ttttttctgt aagaaacaat tgaaatgcca
    33481 tttctccgca agcttccaga tggtggtcga tggttgggaa accaagaagc tcgctgatca
    33541 gcaaattatc gcgtccaatc accactggtg tcttatcttg cagatagctt tgcaagatgc
    33601 ctgctgcaac catatccgaa tttgtaaaga caccatcaac tccaagctct ttaaaaaaga
    33661 agcctgcttg ttttcctttc tctgcatcta tacaatccca aaaaacaagt gattcatcaa
    33721 aagatggaaa aagttgatga catagctgca aagtgatttt tgagttataa cttagcctgc
    33781 cacttcgccc caacgtgatg cccaattgct ttataccttg ctgcttcaaa taagtaagac
    33841 cttctgtaat tgattgttct cgatcaataa agacacagct tgcctgtttt cccttgattt
    33901 cttcacaaaa aactagaggt ccatattttt gatatgccaa taaatcatct agtgtattgg
    33961 cacgagacgt aatgatcaat ccatcgaaag ctttagctgc aaattcttca agataccttc
    34021 gctctttttc tttatgatag cccgtcggta aaagtgtcac tttgtaatca taatcaaacg
    34081 ctgcttcaat gatcccactg atcaactgat caaaatacgg atgattggta taaggcagaa
    34141 tcactccaat gcttttcgtt tttccataac ttaactgtct ggcatttgca ttagggatat
    34201 aatcgacttc tttcatgacc gcaagtattt ctttcctttt ttcctcatct acatatggat
    34261 gtccattgat tacacgtgaa atagtagaaa ctgaataacc tgtgagcttt gcgatatctc
    34321 gaatgtttgc cactgctgct tcgccaccta tacaaaaaga ttttcttgta aatagtctac
    34381 cacagctgga ccgcattcaa aaaactgata catttttcct tcatgcgaaa aactgatccg
    34441 ctgcccgccc caatatatat cttttgtcaa attttcgatt tcattcattg gtatttcttt
    34501 tactatattc ctattcaacc cttgaaaact gtacaacgtt aatacggatc ccttgaactc
    34561 gacaaacgca taaaaataat tgttcgttgt gccatctgtt atctctacat aagtattcat
    34621 tgcaagccct ccttttgata ctttgagtat agtgtatgaa acgcgtctca tagcaaggag
    34681 tttgttttgc caaagaaaag ttttattcct ctttcattat taccaaagtc tcttatttta
    34741 aattgaatac attataatga tactgtaaat atatctttat tattagtgaa t
//
LOCUS       DACESX010000014        33041 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.014, whole genome shotgun sequence.
ACCESSION   DACESX010000014 DACESX010000000
VERSION     DACESX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.014"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            23..853
                     /locus_tag="IXF88_000587"
     CDS             23..853
                     /locus_tag="IXF88_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1507337.1"
                     /translation="MIGLAILLSGCSKWIDRGESITAVGSSALQPLVETVAETYQTEN
                     PGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRVAVVGITPIV
                     NKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRSTFEKWVLDGE
                     TAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTDENVTTNKWT
                     IWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVERDWQGNIVKE
                     "
     gene            968..1888
                     /gene="pstC"
                     /locus_tag="IXF88_000588"
     CDS             968..1888
                     /gene="pstC"
                     /locus_tag="IXF88_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1507338.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1888..2772
                     /gene="pstA"
                     /locus_tag="IXF88_000589"
     CDS             1888..2772
                     /gene="pstA"
                     /locus_tag="IXF88_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1507339.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2787..3593
                     /gene="pstB"
                     /locus_tag="IXF88_000590"
     CDS             2787..3593
                     /gene="pstB"
                     /locus_tag="IXF88_000590"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507340.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3609..4367
                     /locus_tag="IXF88_000591"
     CDS             3609..4367
                     /locus_tag="IXF88_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507341.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4380..5057
                     /gene="phoU"
                     /locus_tag="IXF88_000592"
     CDS             4380..5057
                     /gene="phoU"
                     /locus_tag="IXF88_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1507342.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5294..6325
                     /locus_tag="IXF88_000593"
     CDS             5294..6325
                     /locus_tag="IXF88_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1507343.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6322..7089
                     /locus_tag="IXF88_000594"
     CDS             6322..7089
                     /locus_tag="IXF88_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1507344.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7189..8757
                     /gene="liaX"
                     /locus_tag="IXF88_000595"
     CDS             7189..8757
                     /gene="liaX"
                     /locus_tag="IXF88_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1507345.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9057..9374
                     /locus_tag="IXF88_000596"
     CDS             9057..9374
                     /locus_tag="IXF88_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1507346.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            9374..9724
                     /locus_tag="IXF88_000597"
     CDS             9374..9724
                     /locus_tag="IXF88_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1507347.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9891..10829
                     /locus_tag="IXF88_000598"
     CDS             9891..10829
                     /locus_tag="IXF88_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1507348.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10844..11674
                     /locus_tag="IXF88_000599"
     CDS             10844..11674
                     /locus_tag="IXF88_000599"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1507349.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11718..12743
                     /locus_tag="IXF88_000600"
     CDS             11718..12743
                     /locus_tag="IXF88_000600"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1507350.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12758..13696
                     /gene="galU"
                     /locus_tag="IXF88_000601"
     CDS             12758..13696
                     /gene="galU"
                     /locus_tag="IXF88_000601"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1507351.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13821..14261
                     /locus_tag="IXF88_000602"
     CDS             13821..14261
                     /locus_tag="IXF88_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1507352.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14264..14866
                     /locus_tag="IXF88_000603"
     CDS             14264..14866
                     /locus_tag="IXF88_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1507353.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14925..16031)
                     /locus_tag="IXF88_000604"
     CDS             complement(14925..16031)
                     /locus_tag="IXF88_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1507354.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16241..17242
                     /gene="ccpA"
                     /locus_tag="IXF88_000605"
     CDS             16241..17242
                     /gene="ccpA"
                     /locus_tag="IXF88_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1507355.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17579..19924)
                     /locus_tag="IXF88_000606"
     CDS             complement(17579..19924)
                     /locus_tag="IXF88_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1507356.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20203..21105
                     /locus_tag="IXF88_000607"
     CDS             20203..21105
                     /locus_tag="IXF88_000607"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1507357.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            21318..23093
                     /locus_tag="IXF88_000608"
     CDS             21318..23093
                     /locus_tag="IXF88_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1507358.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23159..24745)
                     /locus_tag="IXF88_000609"
     CDS             complement(23159..24745)
                     /locus_tag="IXF88_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1507359.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            24996..25220
                     /locus_tag="IXF88_000610"
     CDS             24996..25220
                     /locus_tag="IXF88_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1507360.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25362..27113
                     /locus_tag="IXF88_000611"
     CDS             25362..27113
                     /locus_tag="IXF88_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1507361.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27113..28897
                     /locus_tag="IXF88_000612"
     CDS             27113..28897
                     /locus_tag="IXF88_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507362.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29223..30074
                     /locus_tag="IXF88_000613"
     CDS             29223..30074
                     /locus_tag="IXF88_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1507363.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30128..31879
                     /locus_tag="IXF88_000614"
     CDS             30128..31879
                     /locus_tag="IXF88_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1507364.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            31883..32053
                     /locus_tag="IXF88_000615"
     CDS             31883..32053
                     /locus_tag="IXF88_000615"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507365.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(32093..32995)
                     /locus_tag="IXF88_000616"
     CDS             complement(32093..32995)
                     /locus_tag="IXF88_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1507366.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT        10510 a         5865 c         6970 g         9696 t
ORIGIN      
        1 aaaacttaaa ttattcttac caataatagg attagctatt cttttatcag gctgttcaaa
       61 gtggattgat cgaggagaat ccattacggc tgttggctct tctgctttac agcctttagt
      121 agaaacagtt gctgaaactt accagacaga aaatcctgga aaattcatca atgtacaagg
      181 gggcggaagc ggaactggtt tgtctcaagt acagtcagga gcagtcgaca tcggaaactc
      241 tgatttgttt gcggaagaaa aatcggggat cgatgcctca gcattagtcg atcatcgagt
      301 agcagttgtt gggatcactc ctattgtgaa caaggatatt ggcgtaaaag acatcagtat
      361 ggaaaacctg aaaaaactat ttacaggcga gatcaaaaac tggagtgaag tcggtggaaa
      421 agatcagcca gtagttatat tgaaccgtgc ttcaggaagc ggaacccgta gcacgtttga
      481 aaaatgggta ttggacggag aaacagctgt tcaggcacaa gaacaagatt caagcggaat
      541 ggtgcgccag atcgttgccg acacacccgg tgcaatcagc tatgttgctt tttcttatgt
      601 ttccgatgat gtagatacgt tgaatgttaa cggtgtagaa ccgacagatg aaaatgtgac
      661 aacgaacaaa tggacgattt gggcatatga gcatatgtac acaagagatc atccaaaaaa
      721 actgacaaaa gaatttttag actatatctt atctgatgaa gtacaagata acatcgttgg
      781 agaactaggt tatatccctg tttctcatat gaaagtcgaa cgtgattggc aaggaaatat
      841 cgttaaagag taatctttac acaaaattta caaacttagc taaactttta catgttatta
      901 aaaccaaatt gatgttcaat ttgtacactg agtgagtatg ttaaacctga ttaaggggga
      961 gtcatctgtg gaagatgtga aaaaagtatt attaacaaaa tcaaaacgtt caaaaatgga
     1021 acagcgtgga aaattcatca gttttttatg tatcgcgctg atcgtgttgg ttgtattatc
     1081 tattttttat tttgtagcta gtaaaggatt ggccaccttt tttactgata agatcaatgt
     1141 atttgatttt ctgtttggta cagattggaa tccaagccaa atcggagcaa atgggaaacc
     1201 aatggtaggg gcattgccta tgatcacagg ttcgtttatt gtgacctttt tatctgctat
     1261 tgttgccaca ccatttgcca ttggtgcagc agtattcatg acggagatct ctcctaaaaa
     1321 cggaacgaag atcttacagc cggtcatcga gctgcttgta ggtattcctt ctgtcgttta
     1381 tggttttatc ggtttatcag ttatcgtgcc ttttgtcaga agtatttttg gcgggacagg
     1441 gtttggtatc ttagctggta catttgtgtt gttcgttatg atcttgccga ctgtcacaac
     1501 gatgactgtt gatgcattaa aggcagtacc acggcactat cgggaagctt cattagcgct
     1561 tggtgccaca agatggcaaa caatctacaa agtcgtgcta cgagcagccg tgcccgggat
     1621 tttaacagca gtcgtttttg gtatggctcg tgcgtttggt gaagccttag ctatccaaat
     1681 ggtcatcggt aatgcagcat tgatgccaac aggacttact actcctgctt ctacattgac
     1741 gtccatctta acaatgggaa tcggaaatac aatcatggga acagttgaaa ataatgtgtt
     1801 atggtcatta gcgctgatct tgctattgat gtcattattc tttaatatcg tgatccgtat
     1861 gattgggaag aaaggagagc tgaaataatg aacgcaaaac gctcagataa aattgctaca
     1921 ggtgttttat atgtagtttc ggggatcatc gtattgattt tagcagcttt actactttat
     1981 atcttagttc ggggaatacc gcacatttca tggtccttct tgaccgaacc ttccaaagcc
     2041 taccaagaag gcggtggaat cgggatccag ctatttaact cgatttattt gcttttgatc
     2101 acgatgatca tcagttttcc tatttcttta ggtgcgggta tctatctttc agaatatgct
     2161 ggaaaaaact ggctgacaga tgtgattcgc acgtcgattg aaatcttgag ctcattgcct
     2221 tctgtcgttg tcggtttatt cggtttcttg gttttcgtta ttcaattcca gtatggtttc
     2281 tcgattattt ctggtgcatt ggctttgact gtattcaact tgcctctttt gactagaaca
     2341 gttgaggagt ctttacaagc ggtccattac acacaacgag aagcaggatt ggctttaggt
     2401 ttgtcacgct gggaaacagt gataaaagtg gtggtttcag aagcgacacc agggattttg
     2461 actggtgtga tcctaagttc tggaagaatt tttggtgaag cagctgcatt gatctatacg
     2521 gctggacaaa gtgcacctgc gttagatttt acgaactgga atccattaag tgtttcaagc
     2581 ccgatcagca ttttccgtca agctgaaaca ttagctgtcc atatttggaa gatcaataca
     2641 gaaggcacga tgcctgatgg tgtttctgtt tcggctggcg cttctgccgt gttgatcatt
     2701 gctgttttac tgttcaactt cggcgctcgt gcgattggca aacgattata ccgaaaaatg
     2761 acttcagcct aaggagaaga agcaaaatga aaaaatataa ctggaatgaa acaaatgtca
     2821 tcaccatgcc aaaagagaaa gaagtagcac tatatacgga tgatctacat gtctggtatg
     2881 gcgacaatga agcaatcaaa ggagtggatc tggaatttga aaaaaataag atcactgcac
     2941 tgattggtcc ttccggctgc ggaaaatcta cgtatcttcg ttcgttgaat cgaatgaatg
     3001 atgggatcgc taatacgaaa atcactggga aaatcatgta tcaaggcgta gatattaatg
     3061 cgcctcaagt ggatgtttat gagatgcgca aacggattgg catggttttt caacgtccta
     3121 atccgttcag caagtcgatt tatgacaata ttacatttgc tttgaaacag cacggcgaaa
     3181 aaaacaaaaa gaaattagat gaaatcgttg aaacaagctt gaaacaagct gccttgtggg
     3241 accaagtaaa agacagcttg gacaaaagtg ccttggcttt atctggcggg caacagcaac
     3301 gtttatgtat tgctcgtgcg atcgcgatga aacctgatat cttgctgcta gacgagccgg
     3361 caagtgcttt ggaccctatt tcgacaggaa cggttgaaga aacacttgta aacttgaaag
     3421 aagactatac gatcatcatc gtgacgcata acatgcaaca agcggctcgt atcagcgact
     3481 atactgcctt tttctatcta ggaaaagtaa tggaatatga caaaactaac aagatattta
     3541 ccagaccaaa aattcaggca accgaagatt atgtgtccgg tcattttggt taagacggag
     3601 gcctcaacat gacaaaagaa attatttcat ctaaagatct tcatctgtat tatggaaaga
     3661 aagaagcact aaaagggatc gatctaagtt tcaatcaagg ggaaatcact gcgatgatcg
     3721 gtccttctgg ttgcgggaaa tccacttatc tgcgttcttt gaaccggatg aatgacctga
     3781 ttccgaatgt aacgatcacc gggagtgttt tatataaaga caaagacatc tatggtccaa
     3841 aaacagatgt tgttgaacta agaaaagaaa tcggcatggt attccagcag ccaaacccat
     3901 ttcctttttc tatctatgaa aatgtcatct atggattgaa attaaaaggg gaaaaaaata
     3961 aaaaagtcct tgatcaagta gttgaagaaa gcctgaaggc agcgtctgtt tgggatgatg
     4021 tgaaagacaa attgcataaa agtgctttat ctttatccgg tgggcagcag cagcgtgtat
     4081 gtatcgcacg tgttttggca gttaatccgg aaatcatctt attggatgaa ccaacaagtg
     4141 cgcttgatcc tgtttctact gggaaaatcg aaagtatgct gttagaatta agagagcggt
     4201 atacgatgat catcgtgacc cataatatgt ctcaagcttc acgtatttca gataagacgg
     4261 cattcttttt agatggtcac ttgatcgaat acaatgatac gaagaagata ttcatgaatc
     4321 cagaaaaaca agagacagaa gattatattt caggtcgatt tggataagga ggaaacggaa
     4381 tgctacgtac acaatttgaa gaagaattgc tgaatctcca taatcagttc tacgaaatgg
     4441 ggatgatggt gagcagcgcc gtgcacaagt cagttcgtgc atacatcaaa cacgataaaa
     4501 tcttagccca agaagttatc gaaaacgata taaatatcaa taacatggaa acacgcttgg
     4561 agaagaaaag ttttgaaatg atcgcattgc aacagccagt tacaactgat ttgcgtatga
     4621 tcattacggt catgaaagct agctcggatt tagaacgtat ggccgatcat gcggtgtctg
     4681 ttgctaaatc cacgattcgc gtaaaagggc agacgagaat accggaaatc gaaaaagaga
     4741 tctccgatat gtccgattat gtgaaaaaaa tggtggacaa cgtcctagtt gcttatgtca
     4801 aaacagacga aaaagacgca cgtacgatcg ccaatatgga tcaaagagtc aatgactatt
     4861 tcaatcgaat ctataatgca acaatcaaaa atatgcagga aaatcctgag acagtcatca
     4921 gtggaacaga ctacttgaat gttgccagtt atctagaacg gatcggagac tatgtgacaa
     4981 acatctgtga atggatcgtc tacttggcta cagggaaaat cactgagtta aatacgaacc
     5041 acaatgagat tttctaaact aaagcggtga ttctgccgtt tgtttaggaa aagaggttgt
     5101 gaaatcagcg gttgtgaaaa agctgttctg catcaagtaa taagaacagt caaacaaaaa
     5161 tagctcctca tatttttcat ttggctggac ttattaccgc agatgcaagc ttttgaacac
     5221 tgtttattcg ggaaagggtt tgtgacaaaa tatgtcacaa acccttttca tcatttcata
     5281 gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta tttgtgtgct
     5341 tacttcatgg gttattcagc atcaagctgc tttatatgaa gtacctgtca tcgagatcga
     5401 aaaagtacga ataaaagagg atcatcaatt gattacagga agactgatga acacgaatca
     5461 aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag aaacagcaaa
     5521 tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc agctagtagc
     5581 aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg ccatgttgct
     5641 tattggcgga aaacatggtc tatttgcttt aggaagtctc atcatcaata tcgttttact
     5701 tttacttttg ttgctcatct ttactaaaat agaagggatt tctctattaa atatatctat
     5761 cctgttcgtc gttataggga tgattattag catattggct ttagatggat ggaatagaag
     5821 cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt ttatttgtct
     5881 tttgacaatg aatctttgga aagaccaagg actgcgctat gaggaactga attatctgac
     5941 acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg gaggaacact
     6001 cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac aaccagaaat
     6061 aacagcgaaa cagttatggc tttcgggaag gcaagtgggt cgagatgtga ttggctcgat
     6121 gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc ttttatattt
     6181 aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg aatttttacg
     6241 cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc tcttttttct
     6301 gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat tttagcaatc
     6361 agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt attcatcaat
     6421 ttagggatct ttttcttgtt gttattcggt tatcatcaga agtggccgat actggcattg
     6481 tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa cggttataat
     6541 ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt cctactattg
     6601 cttcctatca ctgatttttt agcaatccaa ggttttacaa cgatagaact agaagaatta
     6661 tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc gttgcttctg
     6721 attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg gacattcgaa
     6781 gtatatcagg caaatccgca attatctttt taccaattgc gccattctag tttcgctatt
     6841 gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat gggaagttct
     6901 ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt gatcaatgaa
     6961 aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc actacttgtt
     7021 cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga acaactcaag
     7081 atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt gcataatatg
     7141 gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat gaaagaaaga
     7201 gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg cttagattta
     7261 ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc agacaaggta
     7321 actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact tgaaaacggc
     7381 gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga tcaagaaaac
     7441 ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc gcgtctagat
     7501 gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca ggaagcattg
     7561 atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt agcacagcgt
     7621 cagacgttag aagcagaaat caaagagtta gaagctagtg gcgatacact gttagaagaa
     7681 caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc tgagaaaaaa
     7741 gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc aacagataca
     7801 ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggaaa attcttgaag
     7861 aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa agatgtcagc
     7921 ttgcgtgttc ctgggattgc gacaacgaaa tttgagcatg aattttacta tgaagctcct
     7981 gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa aacgtgggac
     8041 agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg tgctgaacca
     8101 tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat ctccttccaa
     8161 attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg agtatacgac
     8221 cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga agctaaagat
     8281 atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc aacgatgctg
     8341 gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt agattctatt
     8401 atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct ttctgtgtct
     8461 ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa agtagaagca
     8521 agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt ggaaggacat
     8581 gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt agttcgtgag
     8641 aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg tgaaatcgcg
     8701 caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga taaataaaga
     8761 ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa atagcttccc
     8821 ctattttaca aaattgtgcc ttattgccga taggctatct cttgaacacc gtttaccaag
     8881 agattatgaa cttgccaatt atcgccgagt cacaaggaat aatttcaaaa aaagctcgtc
     8941 acgttttatg aatatggtaa tttgtgaccg agaattttgc aaaaagacac cgattaagaa
     9001 gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga gacatgatga
     9061 aaaaaaagtt gacaaaatcc aataaaaatg ttgttttgac agggacttta gcaggaatag
     9121 gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc ctgagcttag
     9181 ttgcttttgg aagtccaatc attttataca ttttattagc tttgattatc ccatctgcgc
     9241 gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac aattatcgtg
     9301 aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat gatgacgaat
     9361 ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa cattcatatc
     9421 attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg ccgttcttgc
     9481 cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga tactgtccct
     9541 gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag cgattcttaa
     9601 aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg gtgcagcgtt
     9661 gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata acagtagaaa
     9721 ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc aggaatgcgt
     9781 tttctattac attggtaatt aaataaaaac gtttttttta attggtagta cttttggcta
     9841 gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga atgtcagaag
     9901 ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca ggcgacgaga
     9961 aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta gagttgactg
    10021 gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa gaaatcagtt
    10081 ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta tgtagtgaag
    10141 atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg atccaggcag
    10201 caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgc ttattaggag
    10261 aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac ggtgttttag
    10321 tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga aaaagtgaaa
    10381 cagcgttgga gttgatcaaa cgcggtcatc gattgatcgc agacgatcga gtagatgttt
    10441 atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa catctgattg
    10501 aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt gcggtacgtg
    10561 gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat aagaaatacg
    10621 accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtaggcatt ccacaaataa
    10681 aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc gcgatgaatt
    10741 tccgtgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga ttaagtcgct
    10801 tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag cacaaatcaa
    10861 tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga tcatcgtatc
    10921 aggtatcatt atcgccatgt ggcttagcag cagagaggct gttcgggtag gattaaaagc
    10981 agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg gcgctcgctt
    11041 atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa tattcaatat
    11101 ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca cgctgtattt
    11161 ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg caccaagtgt
    11221 attattagca caagcaatcg gtcgttgggg aaatttcatg aaccacgaag catatggacc
    11281 agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca ttgataatat
    11341 gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt ggagtttgat
    11401 tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa aaggtgaagt
    11461 ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag gattacgtac
    11521 agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg ctgtgttatt
    11581 tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac cggattatga
    11641 ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa aatgaaaaaa
    11701 atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc tcttggggta
    11761 ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg ggacatcgtg
    11821 cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta ccagatcatt
    11881 gtctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa gacgcagatg
    11941 cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa ctagtgccta
    12001 aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag gggacacata
    12061 aaaggatctc tgatatcttg ttagaggaaa tcccagtaga aaaaagaaaa gctgttgttg
    12121 ttctttctgg accaagccat gcagaagaag tagcgaaaca tgacatcact acgatcacag
    12181 ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg aataattatt
    12241 ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct ttgaaaaata
    12301 ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat gcaaaggcag
    12361 caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg ggtgccaacc
    12421 cgctgacctt tatcggatta agcggagtag gcgatctagt cgttacttgc acaagtgttc
    12481 attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta gaagaggtgc
    12541 tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg atggaactag
    12601 cccaaataat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg ctgtatgaac
    12661 aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatgggaaa aaagaaaatg
    12721 aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagtcaaaa aagcagttat
    12781 tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg caaaggaaat
    12841 gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt tgaaatcagg
    12901 tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag atcactttga
    12961 ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat tattgaaatt
    13021 agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc caaagggttt
    13081 aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg tcgttatgct
    13141 aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg atgattacga
    13201 acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa cttcaaaata
    13261 cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca aaaactttgt
    13321 tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac gttacttatt
    13381 gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa atgaaatcca
    13441 actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc gtaaattcac
    13501 aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta ttgaatatgg
    13561 tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt taggtaaaac
    13621 attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag aaatcaaaga
    13681 agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga ataaaaagta
    13741 gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat agaaaagaga
    13801 gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc gcagcaatcg
    13861 cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct aaaactttag
    13921 aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc acaaaagacg
    13981 tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa ctgctcttgg
    14041 atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct gatttaagtg
    14101 aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc ggcggaatcg
    14161 gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg atgaaatttt
    14221 ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta aaaaaggtgg
    14281 atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt tattgttagc
    14341 accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg atttgatgga
    14401 tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa caggtattgc
    14461 aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta gtgtcaaaca
    14521 aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct tgttaaatct
    14581 taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag atatgaaaaa
    14641 acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca tgattgatgt
    14701 aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag aagcaaaacc
    14761 agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga aagaagctaa
    14821 aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc acaaatttat
    14881 agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt ttctaaaaca
    14941 agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac atatacacag
    15001 tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag agagaagcac
    15061 atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc gtggacggtt
    15121 gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata gctgttgatc
    15181 acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac ggcttcttgt
    15241 gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc ggttggtttt
    15301 tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac gccaagatcg
    15361 aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg agggcttgca
    15421 gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc ttgttttttc
    15481 aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt cactgcttta
    15541 aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt gtctttttcc
    15601 tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt gtaagaatcc
    15661 gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc gttcttttca
    15721 atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat gatctcccaa
    15781 gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt ttgagcatct
    15841 tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat aaaaagagct
    15901 agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg gccagggtcg
    15961 ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc attgattttt
    16021 acgttattca tttggcaacc tactttctaa attaattttg tattcaataa ttgtaccact
    16081 gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc atgaaaacag
    16141 agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa tgtaagacgt
    16201 attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc aaacaatcac
    16261 tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac gtgtcgtaaa
    16321 tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta tcgatcgctt
    16381 ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa caacagttgg
    16441 tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg gtatcgatga
    16501 tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca acgaccaaaa
    16561 agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga tcttcatggg
    16621 tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc ctgttgtatt
    16681 agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt atgtatccgc
    16741 aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg cttttgtcag
    16801 tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata aagaagcatt
    16861 aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat acaacttcaa
    16921 agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg tctatgtttc
    16981 agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg tgaaagtacc
    17041 ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag ctcgtcctcg
    17101 tttaacaagt atcacacaac cattatacga tttaggggct gtatctatgc gcctattgac
    17161 gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt atggaatcga
    17221 agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt gcctgccgtt
    17281 taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga tgaatattgt
    17341 gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg gttatgacaa
    17401 aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt gaaattacat
    17461 ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac catcaaaaaa
    17521 ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt ctattaaatc
    17581 aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa gctgaagcgg
    17641 tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta aaggcgcttt
    17701 tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt tggatggatt
    17761 ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact tttttcttca
    17821 tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt tttgcttggt
    17881 aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct ttgctgtcca
    17941 ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga gtagagacaa
    18001 ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa tctgcttttc
    18061 ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt tttgcattga
    18121 tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag atccggacag
    18181 agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa tcgatgatat
    18241 agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc tgttgaagcg
    18301 tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca tttgccgggt
    18361 agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg gaattcaaca
    18421 catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac tcacggacgg
    18481 tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca gcattttctc
    18541 cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata agccccatgt
    18601 cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga cgttctgtat
    18661 cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat ccgtagatgc
    18721 ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca gcaccatcat
    18781 ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca tctttatcga
    18841 ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt tgataataag
    18901 cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat tgttgtgcaa
    18961 tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca gtctgttctg
    19021 cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct tcgtattgtt
    19081 ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt ctggcaaggc
    19141 cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac gtgatcgggc
    19201 tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt gcatctacac
    19261 caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta ttattacgac
    19321 caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag tatttttcta
    19381 cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca tctgtaagga
    19441 tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca ataccagtag
    19501 cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt ttatcgaagt
    19561 attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta tcagatgaaa
    19621 tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct gcataaacaa
    19681 tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta gtcggcaatt
    19741 gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct agagctgcga
    19801 acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta aaaataatct
    19861 taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta tttttgcgtg
    19921 gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat aatagaacaa
    19981 aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta aaacgtgaga
    20041 aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt tcagttacta
    20101 tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta ccaatatata
    20161 ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt aggctcacac
    20221 gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc cagttatgga
    20281 tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa agccatcgaa
    20341 gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac aaatatcgtc
    20401 gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat gtttcctttt
    20461 gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc tactcaaatt
    20521 acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc tagaattatt
    20581 gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt ggagacaatg
    20641 gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat catcgatgga
    20701 gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa tgatgccggt
    20761 tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat catcgggtta
    20821 gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc gcataaagac
    20881 cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa gatcgtccat
    20941 gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg caaagataaa
    21001 aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca aacattcgat
    21061 ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt tctatctgac
    21121 agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa acaaaggagt
    21181 actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat ttagacaaac
    21241 aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg gcaaaacgtg
    21301 attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa ctaaaaaaga
    21361 aacatacata ctttattctt ggcatagccc tattatttac aattattcgt ttgtatttat
    21421 atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag ctgctgattc
    21481 gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact acaacacttt
    21541 ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat ctttcttatg
    21601 cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc gcgtttaaac
    21661 cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg ttttctccga
    21721 ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg attccggctg
    21781 ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt tctagcagaa
    21841 aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg ttcattcgcg
    21901 aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt ttcgtccaga
    21961 tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa cgactctttc
    22021 ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata gttggctttt
    22081 ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt gcaaccatca
    22141 atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga atagatgatg
    22201 aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca ctaaataaag
    22261 cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa ttatatacga
    22321 ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc atcttttggg
    22381 cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa gaaaccaatg
    22441 atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga acaatcaaaa
    22501 agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt gatctgccaa
    22561 tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa gatttccatc
    22621 aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa gaattattaa
    22681 accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct aatttccaat
    22741 taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt ctggctattc
    22801 ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc gtatttgcaa
    22861 aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta atactgacac
    22921 aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa aaaaaggact
    22981 attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc gtagtaacaa
    23041 gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa taaaaagacc
    23101 cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa atcagttttt
    23161 attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt cctttatata
    23221 aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca taagcgatgt
    23281 gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt tcgatctgtc
    23341 ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag ccaagaagtt
    23401 ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg gtcatcatca
    23461 gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat tcattcatta
    23521 agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac aaaacacaaa
    23581 tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa atcgggatac
    23641 gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta atagtagagt
    23701 tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca gattttgcta
    23761 cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca tacagaccaa
    23821 aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg atcaatagca
    23881 gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta gaaaaacgta
    23941 cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt gaaactgctg
    24001 aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa gctccagtca
    24061 gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta gggataaagc
    24121 cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca aaaacgaata
    24181 aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg acttttgtca
    24241 gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc aatggaatat
    24301 caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct gttgccataa
    24361 tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg cgtgctcgga
    24421 ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata tactgtaaaa
    24481 gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg aaattttgtc
    24541 ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct actaacgcac
    24601 caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt acatcaactg
    24661 ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga tgcttatgaa
    24721 attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc tacgattaca
    24781 ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa ttttttttca
    24841 tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa ccactatttt
    24901 ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa aataagtcat
    24961 tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt attgtcgtac
    25021 ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta gcgcgtaaat
    25081 atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg cgatcaatga
    25141 tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg cagcaaatga
    25201 agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga ttttttcagt
    25261 ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa agacaagcac
    25321 agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt tttagaaaat
    25381 taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc tcgctattcg
    25441 cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt gatgagatcg
    25501 ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg attatcgctg
    25561 cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga agtgcagctc
    25621 gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg gacaatctct
    25681 tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat ttgaatgcga
    25741 tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt acgggcggaa
    25801 caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt gcgctgattc
    25861 ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat gatgcttttc
    25921 gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt atcactggga
    25981 tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt gaagaaaaga
    26041 ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta tttgatccat
    26101 tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt ggaaagtatg
    26161 tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat atcggtatgc
    26221 ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga ggaaatgctt
    26281 cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa caactgggtg
    26341 cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt aattatccga
    26401 aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga gaaacattag
    26461 gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg atgcgtgaat
    26521 acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa tattcattga
    26581 atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt tcgatgacca
    26641 ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta gagcatgcgg
    26701 cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat gataccttag
    26761 ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg attgctcgag
    26821 cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca gtagatgcca
    26881 aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa acaactatca
    26941 ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt gtcgatcaag
    27001 ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc tggtatgaac
    27061 aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc tgatggatga
    27121 aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa agatcatcaa
    27181 acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta tcttatttgc
    27241 gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt ttatggatga
    27301 tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg gtatgtactt
    27361 atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt attccatggc
    27421 ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc atacactagg
    27481 tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga cgaatgacac
    27541 agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga tctttgcagt
    27601 cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt ggactttggt
    27661 gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt ctcgtattta
    27721 ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat cgatttccgg
    27781 catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat ttgaacaagt
    27841 gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga ttctgttaag
    27901 tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt tgtttgggat
    27961 cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga cctatgtcca
    28021 gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca cagatgggat
    28081 ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac cacaacaaaa
    28141 ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg taagcttttc
    28201 ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc cgggagagac
    28261 cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg tattgatgcg
    28321 gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc gagaatatcc
    28381 aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct tgttttatgg
    28441 ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac agatacgcca
    28501 tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag gatatcactc
    28561 aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga tcacttttgc
    28621 gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag caaatatcga
    28681 tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag gacggacaac
    28741 gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt tagttttaga
    28801 caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa atggtttgta
    28861 tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac agtagaattt
    28921 tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat ctgtgagagt
    28981 tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag tgcctaatag
    29041 ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa acgatacaac
    29101 ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt ttcatggacg
    29161 gctttttggg tttttctttt tgttttggat acagccctat acatcatgga aaggagcaaa
    29221 acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat gaaagaaatc
    29281 aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt gacccgattt
    29341 ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca caacagttcc
    29401 aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa atcattgaat
    29461 atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca ttgacggatc
    29521 atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat gtattgctgt
    29581 atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca gtgaagaaaa
    29641 tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc gcattgcatt
    29701 tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt actataacaa
    29761 tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat gaagattcag
    29821 tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc ctagatggag
    29881 aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa aaaaaatata
    29941 aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg ttcaactacc
    30001 agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta gtgcatgaga
    30061 atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata agggtagtga
    30121 cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg gaaaatacca
    30181 agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca atggtttgac
    30241 acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa atgatgagat
    30301 cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt atcaagttgt
    30361 cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa accttacttt
    30421 tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg atggaatatc
    30481 gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc gtggactgac
    30541 agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg ttatgtttga
    30601 taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa cagctgcccg
    30661 taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa tgaatcctag
    30721 cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca tattttctgg
    30781 cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga ttgcgcgtaa
    30841 ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc aaattcagaa
    30901 accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat tcttcaccgt
    30961 tcttattaca atgcctttag gttttttact cgttggacca gtattcacat tcgcaactga
    31021 tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt atggagcaat
    31081 cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa tcgtaccaat
    31141 gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg ttgcagtagt
    31201 gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa atccaaaaga
    31261 caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag agccagctat
    31321 ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg gtggtgcgtt
    31381 agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg gactaggcat
    31441 ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga ttaatgtatt
    31501 gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct tttggaagga
    31561 tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg taaaaacacc
    31621 gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg cacaaggaac
    31681 actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc cttttgacgg
    31741 aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg aaacagggct
    31801 ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat attttgaaag
    31861 cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct tcgacaaaaa
    31921 gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca attacacgga
    31981 ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg cacttatcac
    32041 cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct ttttattcca
    32101 cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata gggcgtttat
    32161 cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt tctgcaaaaa
    32221 cacaactata taacacgtct agaagataac aaattgatgt attgatcgta aaattaccaa
    32281 tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc attgcagata
    32341 gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt ttcaatattt
    32401 ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc ccgcacatat
    32461 cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca atcgtgacat
    32521 ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta ttcgagttac
    32581 tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt tgcagttggt
    32641 catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa gccattttat
    32701 ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct acgtcttgaa
    32761 aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg acccagccat
    32821 caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa catcgctcag
    32881 caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga aactggatca
    32941 cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat aacatgtaga
    33001 taccttcttc ttattttttt acgtaaatta taactttttt g
//
LOCUS       DACESX010000015        32426 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.015, whole genome shotgun sequence.
ACCESSION   DACESX010000015 DACESX010000000
VERSION     DACESX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.015"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            62..430
                     /locus_tag="IXF88_000617"
     CDS             62..430
                     /locus_tag="IXF88_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1507367.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            600..1031
                     /gene="mraZ"
                     /locus_tag="IXF88_000618"
     CDS             600..1031
                     /gene="mraZ"
                     /locus_tag="IXF88_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1507368.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1052..2011
                     /gene="rsmH"
                     /locus_tag="IXF88_000619"
     CDS             1052..2011
                     /gene="rsmH"
                     /locus_tag="IXF88_000619"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1507369.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            2016..2417
                     /gene="ftsL"
                     /locus_tag="IXF88_000620"
     CDS             2016..2417
                     /gene="ftsL"
                     /locus_tag="IXF88_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1507370.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPESSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2418..4610
                     /locus_tag="IXF88_000621"
     CDS             2418..4610
                     /locus_tag="IXF88_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1507371.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGMFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4636..5598
                     /locus_tag="IXF88_000622"
     CDS             4636..5598
                     /locus_tag="IXF88_000622"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1507372.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5612..6973
                     /locus_tag="IXF88_000623"
     CDS             5612..6973
                     /locus_tag="IXF88_000623"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1507373.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6999..8087
                     /gene="murG"
                     /locus_tag="IXF88_000624"
     CDS             6999..8087
                     /gene="murG"
                     /locus_tag="IXF88_000624"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1507374.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8102..9322
                     /locus_tag="IXF88_000625"
     CDS             8102..9322
                     /locus_tag="IXF88_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1507375.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9478..10803
                     /gene="ftsA"
                     /locus_tag="IXF88_000626"
     CDS             9478..10803
                     /gene="ftsA"
                     /locus_tag="IXF88_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1507376.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10826..12067
                     /gene="ftsZ"
                     /locus_tag="IXF88_000627"
     CDS             10826..12067
                     /gene="ftsZ"
                     /locus_tag="IXF88_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1507377.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12084..12761
                     /locus_tag="IXF88_000628"
     CDS             12084..12761
                     /locus_tag="IXF88_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1507378.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12775..13374
                     /locus_tag="IXF88_000629"
     CDS             12775..13374
                     /locus_tag="IXF88_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1507379.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13389..13676
                     /locus_tag="IXF88_000630"
     CDS             13389..13676
                     /locus_tag="IXF88_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1507380.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13725..14507
                     /locus_tag="IXF88_000631"
     CDS             13725..14507
                     /locus_tag="IXF88_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1507381.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14528..15235
                     /locus_tag="IXF88_000632"
     CDS             14528..15235
                     /locus_tag="IXF88_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1507382.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15497..18295
                     /gene="ileS"
                     /locus_tag="IXF88_000633"
     CDS             15497..18295
                     /gene="ileS"
                     /locus_tag="IXF88_000633"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1507383.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18344..19870)
                     /locus_tag="IXF88_000634"
     CDS             complement(18344..19870)
                     /locus_tag="IXF88_000634"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1507384.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19885..20532)
                     /locus_tag="IXF88_000635"
     CDS             complement(19885..20532)
                     /locus_tag="IXF88_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1507385.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20716..21045)
                     /locus_tag="IXF88_000636"
     CDS             complement(20716..21045)
                     /locus_tag="IXF88_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1507386.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            21222..21950
                     /gene="sfsA"
                     /locus_tag="IXF88_000637"
     CDS             21222..21950
                     /gene="sfsA"
                     /locus_tag="IXF88_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1507387.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21966..22979
                     /locus_tag="IXF88_000638"
     CDS             21966..22979
                     /locus_tag="IXF88_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1507388.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22979..24256
                     /locus_tag="IXF88_000639"
     CDS             22979..24256
                     /locus_tag="IXF88_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1507389.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            24319..27021
                     /locus_tag="IXF88_000640"
     CDS             24319..27021
                     /locus_tag="IXF88_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1507390.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYQ
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            27173..27490
                     /locus_tag="IXF88_000641"
     CDS             27173..27490
                     /locus_tag="IXF88_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1507391.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            27520..27840
                     /locus_tag="IXF88_000642"
     CDS             27520..27840
                     /locus_tag="IXF88_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1507392.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            27969..29408
                     /locus_tag="IXF88_000643"
     CDS             27969..29408
                     /locus_tag="IXF88_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1507393.1"
                     /translation="MEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSVAVMINAI
                     IRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFSWGYNLAR
                     AYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGATIADGTLS
                     VGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAVSKAFAAI
                     LPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIVTVFVQVF
                     WFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGKAYMWVRG
                     SFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMFGLPIVLN
                     AIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGADWRAPIVT
                     LVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            29476..29697
                     /locus_tag="IXF88_000644"
     CDS             29476..29697
                     /locus_tag="IXF88_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507394.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            29728..29910
                     /locus_tag="IXF88_000645"
     CDS             29728..29910
                     /locus_tag="IXF88_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1507395.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            29910..30323
                     /locus_tag="IXF88_000646"
     CDS             29910..30323
                     /locus_tag="IXF88_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1507396.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            30446..31627
                     /locus_tag="IXF88_000647"
     CDS             30446..31627
                     /locus_tag="IXF88_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1507397.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            32016..32089
                     /locus_tag="IXF88_000648"
     tRNA            32016..32089
                     /locus_tag="IXF88_000648"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32050..32052,aa:Arg,seq:cct)
     gene            complement(32158..32400)
                     /locus_tag="IXF88_000649"
     CDS             complement(32158..32400)
                     /locus_tag="IXF88_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1507398.1"
                     /translation="MRKKLKELGIEKQFTLHGLRHTHASILLYQGVNILSVSKRLGHS
                     SLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        10850 a         5560 c         6889 g         9127 t
ORIGIN      
        1 aacagttgtg taaagtatat acagtatact tgaagcaacg ataaaaaagg agtttacgtt
       61 catgggcaca ttcacaccac ttatcttgtt ttggtatatt tacccggtaa tcgttttgtt
      121 tggttgccaa tttcttgtaa aattattttc gttaaatcgt cgttttaaaa taaaagcccc
      181 tgatttggcg attccttttt tgtggggcgg tatgcatgca ttgtctagaa atacagggac
      241 aatttctatc ttaccgtttt tctttatttc tgttttgcta atgggaattt gtattgctgt
      301 tttccaagca tattattatg aagaaattat ttatccgcgt tatttcaaga tgacttggcg
      361 tttggttttt ctgttgacaa tcgtcttgta cgctgtcttg atcattgtga acatactttc
      421 ttacctatga aaaaaataga aaagctgtaa aaagtctttt aaaaggcttt ttgcagcttg
      481 atttttaaaa aaatgtttat gaataaaaaa agttgttccc ggatttccca gaaaaggtgg
      541 tagaaagtgg gggattgtgg tagactaatt ttagttagtg gaggaatggg gggctttcaa
      601 tgttgatggg tgaattccaa cataatattg atgcaaaagg acgtttaatc gtgccctcaa
      661 aacttcgtga agaacttggc gaaaagtttg ttctgacacg cggattggat ggctgtcttt
      721 tcggctatcc gatgagcgaa tgggaaaatc tggaagctaa actgaacgaa atgccgttgg
      781 ctaaaaaaga cgcacgtact tttgtgcgat tcttctattc ggctgccact gaatgcgaac
      841 ttgataaaca aggacgaatc aatataccca gcacgttaag aaattatgca gctttgacaa
      901 aaggttgcgt agtcatcggt gtatctaacc gaattgaaat ttgggatgag acacgatggc
      961 aagatttttc tgcagcagca gaagagaact ttgacgaaat tgctgaaagt atgattgatt
     1021 tcggactata agaataggaa gaggctgact aatgactgaa acatttcaac actatacagt
     1081 catgttgaaa gaaacggtag atggattgaa catcaaaccg gatggcgttt atgtagactg
     1141 tacactcggc ggtgccggac atagtgaata tttattaagc caactaagtt ctaaagggca
     1201 tctgtatgcg tttgaccaag atcaaaaagc aatcgatcac gccaagctgc gtttagcttc
     1261 ttatgtcgaa caaggtatgg tcacatttat caaagcgaac ttcagagaat tagaagaacg
     1321 cttgagtgag catgtagata aggtagacgg cattttgtat gatctagggg tttcctctcc
     1381 tcagctagat gaagcagaac gtggatttag ttatcatcaa gatgcaccgt tggatatgcg
     1441 catggatcag tcagctcctt tatccgctta tcacgtaatt aatgaatatt cctaccatga
     1501 attagtaaag attttcttcc gctatggcga ggagaaattt tccaaacaaa ttgctcgtga
     1561 aatcgaacga gtacgaaaaa caaaaccaat ccaaacaact ggtgagcttg tcgaaatcat
     1621 taaacaagtc atcccagctc ccgcaagaag aaaaggcggg catcctgcaa aaagaatttt
     1681 tcaagctgtg cggattgcag taaatgacga actcggtgcc gaagaggcct ctttagaaca
     1741 ggcaatccgg ttattgaaag tcaatggaag aatcagcgta atcacattcc attcattgga
     1801 agacagaatt gtcaaaagta tgtttaaaga atatagtacg gtacaagatc taccaccggg
     1861 acttcctgtc atacctgacg aattccaacc agaactgaaa gtcattactc gtaaaccaat
     1921 ccttccttca gaggaagagc taacagaaaa caatcgatca cgcagtgcaa agttacgtat
     1981 tgcagaaaaa ataagaatga aagaggagta gaatgatggc agaattaaag aaaatgcaag
     2041 attacgaata tgatttacct gaattggtgg atcagccgga acaacaaatg cctgaatctt
     2101 cttcgaatca agaaatcatt cttccacaat caccggcaaa acgattaaag cagatttctg
     2161 ctttggaaaa aattgctgtg gcaagcgtgg cgattgctat tgttgcttta tgtgttttaa
     2221 cagttatgct gcgcacaaat atcagcggag ctgaaaaaga aatcacgtca atacaaacag
     2281 aaatcaatga taaaaagcaa gaaaaaacga gtttagaaca agaaaaaaac gaactttctc
     2341 gtactgaacg gattaaacaa atcgcagaag aaaaaggatt gtctattaat gacgacaatt
     2401 taaggaaagt gaagtaaatg agtcttaaaa ataaaattcg gcggtttatg gaaaagaaaa
     2461 atctaaatcc gatgaacaat cgcaaaaaag taggtatcat tttatttgct acgagtattg
     2521 gaatgttctt tttatttgcc gtccgtttct cgtatatcgt tattggaggt catgttgctg
     2581 gtacctcatt agctgaaaaa acaaaacagt tgtaccaagg aagtgaagta gtaaaagcaa
     2641 aaagaggaac gatttatgat cgtaacggtg tggcgctcgc agaagatgcc tcctcttatt
     2701 caattaaagc gatactctca aaaacatata cctctgggga taaaaaactg tatgttgaag
     2761 aaaagaattt tgataaaata gcagaaatcc tgcataagaa tctttctata gacaaaaaag
     2821 atgcactgaa tattttagag gacggcgcaa aaaaagagct gtatcaagtt gaatttggta
     2881 gctatgggaa aaatatctcc caagaaacca agcaaaacat cgaagcggac atgaaaaaag
     2941 aaggtgtcgc tggtctttat tttgtcgacc atcaggctcg gatgtatcca aatggcgtct
     3001 tttcttctca ttttattgga tacgcagttc cagacaaaga tgagaatggc ttagttggta
     3061 aactaggttt ggagtctgct tacaatgata tcttgagcgg taaagatggt aagattattt
     3121 atcaaaaaga taattttcaa aatcctctgc caggtacagt ggcagaagaa gaaaaagctg
     3181 tcgacggaca agatatctac acgactttag acagcagact acaaagttat ttagaaacac
     3241 tgatggatca agtaaatgaa gaatatcagc cagaagaatt gacagcagtc ctgatgaaag
     3301 caaaaaccgg agaaatcttg gcaatgggac aacgacctac tttcaatcct gaaacaatgg
     3361 aaggattgac tggaaaagat gctatctggc gtaatttctt ggtacaagat agttatgaac
     3421 caggatcaac catgaaagtt ttcactactg cagctgctat cgaagaagga gaattcaacg
     3481 agaatgaaac cttccaatct ggaaaaatcc aagtagaaga tgctacgatc aatgaccatg
     3541 actttggtga aaaaggtgtg ctgaccatgc gtcaagctct ttcatggtca agtaacgtcg
     3601 ggatggtcat actggaacag cgattaggcg gacgatggta taactattta caaaaactgg
     3661 gatttggaca aagtactcat tctggattag atgatgaagt aaacggtgct ttacctacat
     3721 taaatattgt cgaccgagca atgagtgctt atggacaagc ggtaggtgtg acgaatttcc
     3781 aaatgatgaa agctttcact tctattgcga ataacggtac aatgattcaa cctcgatata
     3841 tcagcaaagt ggtggatccc caaactggag aagagcgaac aactcaaaca gaagttctag
     3901 ggcaaccttt ttcaaaagaa acaacagaaa aagtccgtga atatatgaga gatgttgtcg
     3961 aaagcgaaaa ctacggtagt gcttatggtg tatatagtgt acctggatac aatgtatcgg
     4021 caaaaaccgg aacagcccaa atcgcttctg atacaggggg atatcaaaca ggagatacag
     4081 cctacctgta ttcgattgtg gagatggttc cttctgaaga tccagactat gtcttgtatc
     4141 taacaatgaa acaccctaag acatatgatc gaatggcatt ggctaaaatc gcaaatccat
     4201 tgatgaaacg ggcaatggat tttaaagaaa ctgaagaaga ttcagataca gaaacaaaaa
     4261 cagaaaaagt atccgtagcc gattatcgta atcttgaagc ggacgttgca gcagctgatg
     4321 ctcaaaaaag tggactgcaa cctgtagtga tcggtaatgg taaaaaagta caaaaacaat
     4381 ctactgccaa cggtgatcag ttgatctctg gagaaaaact gatcctatat actggtggtg
     4441 ataagctgat gcctgacgta acaggatggt caaaagcaga tatcatgaag ctggggaaaa
     4501 tcctcggtat tgaagttagt tttgatggag atggttattg tgtcaaacaa gaacttgctc
     4561 catatgaaaa aatcactgaa gacaaactta gttttacttt agaagaataa gaagagaaat
     4621 aggagagaaa agagcatgga atggacacaa gcattgattc ccattgtcag cagttgtgca
     4681 atgacaattg ccgcaatgcc gctatttatt ggttattttc aaatgaaaaa gcaaggtcag
     4741 gcgattcgtg aggagggacc aaagtggcac aatagcaaag ccggaactcc tacaatgggc
     4801 ggcttagtgt ttctgatcgg ctctattttg acaggaattt gggttggagc ttggcaaaaa
     4861 cagctaactc ctactctttt catcttgctt tttgtattag cgttatacgg agtaatcggc
     4921 tttcttgatg attttataaa aatattcaag aaaagaaaca tgggactgaa ctcgaagcag
     4981 aaattgattg gacagattct agggggaatc atcttttatc ttgtttaccg ttctgaaggt
     5041 tatccaggtg tattgaattt cttcggacta gatctacctt taggaatcat ttatggtatt
     5101 ttcgctctct tctggcttgt tggattttca aatgcagtaa atctgacaga tggcatcgat
     5161 ggattagttg ctggcttagg aagtatctct tttgcagctt acggtctaat tgcttggcat
     5221 cagcatcagt atgatgtatt aatcatttgc ttaagtgttt taggaggttt actgggtttc
     5281 ttcgtctaca acagaaaacc tgccaagatt tttatgggag atgtcggttc attagcgttg
     5341 ggcggacttt tagcggcaat ttctataatg ctaaaccaag aatggacatt gcttttgatt
     5401 ggtttgatct atgttatgga aacagcaagt gtgatgcttc aagttacttc ctttaagctt
     5461 acaggaaaac gaatttttaa aatgtctcca attcatcatc actttgaaat gtgcggatgg
     5521 tcggagtgga agattgatac agttttttgg ttgatcagca ttgtgacttc gttgatcaca
     5581 ttatggatcg tttggtagga gatgacggga aatgaaaaaa atatcaacat atgaaaataa
     5641 gaaagtgctt gtcttaggac tcgcaaaaag cggagtaagt gcggccaaat tattacatga
     5701 attgggcgca cttgtcaccg ttaatgatgg aaaacctttt gacgaaaatc cggaagcaca
     5761 agagctgctt tctttaggga taaaagtaat aacaggtagt catccaatcg aattgttgga
     5821 tgaagaattt tcattgatgg tcaaaaatcc agggattccc tacagtcatc ctttagtagc
     5881 aaaagcccaa gaaatgggta ttccggtaat tacggaagtt gagctagctt acgaagtagc
     5941 ggaatgccca attattggga ttacaggaac gaacggaaaa acaacgacaa caacgatgac
     6001 aggactacta ctgaatgcag gagcagatca aggaattgca cgtttagcgg gtaatattgg
     6061 gtatccagca agtggtgtag cgcaagaagc aaaatcagaa gataaaattg tgatggaatt
     6121 gtctagcttc caactcatgg gaatcacaga tttccgccct catatagcgg tgattaccaa
     6181 tatctatgaa gcacatattg attatcatgg tacaagaaaa gaatatgtca aagctaaatg
     6241 gaaccttcaa aaaaacatga cagaaaaaga ttatctgatc cttaactgga atcagtcaga
     6301 actacaagaa ttagcgcaaa gaacaaaagc acgcgtactg cctttctcga caaaagaagt
     6361 gttggaagac ggtgtgtatg ctgacgatta ctcaatatac tataaaaaag aaaaaataat
     6421 ggaaatatct gaacttggcg taccaggtaa gcataatgta gaaaatgcac tagctgctat
     6481 ttctgtagca aaattgtatg gtatatcaaa tgaagctata cgagaaacgt tgcatttttt
     6541 ccatggcgta ccacatcgta cgcaatatgt aggagaaatc caaggcagaa aattttataa
     6601 tgattcaaaa gcaaccaata ttttagcgac taaaatggct ctaagtggat ttgaaacgtc
     6661 taaggtcgtg ttattggctg gcggattaga cagaggaaat acatttgatg aattaattcc
     6721 ttcattaaaa ggaatcaaag caatggtcgt atttggccaa acaaaagaaa agttgatgga
     6781 tgctggaaaa aaagcaggca tcgagacaat agtaacagcc gattctgtag aacaagcagt
     6841 tcctttagca ttagaaaaca gtacagatgg tgatgtagtt ttattatcac cagcgaatgc
     6901 cagctgggat caatacccaa attttgaaac tagaggaaac agattcatgg aagctgtgaa
     6961 ccgattaaaa tagaaaaagt agaagcgagc gataaaaaat gaaaattttg gttacaggcg
     7021 gaggaacagg gggacatatt tatccagctc ttgcctttgt caattatgta aagactcaag
     7081 aaccgaatgc tgaattcatg tatgtgggag caaaacgagg tctagaaaac aaaatcgtcc
     7141 ctgatacagg aatgcctttc cataccttgg agattcaagg atttaaacga aaaatttcca
     7201 tgcataacat caagacggtc cgattgtttc ttaagagtat ccgtcaagca aaaaaaatcc
     7261 tcagtgattt tcaaccagat atcgttatcg gtactggagg atatgtttcc ggagcggtag
     7321 tgtacgctgc agccaaaatg ggtattccaa cagttatcca tgaacaaaac agtgtaccag
     7381 gtgttaccaa taaattttta agccgatatg tggatcggat cgctctttca tttaaagatg
     7441 cggcttcctt ttttcctgaa aacaaagcag ttcttgtagg taatcctcga gcacaagaag
     7501 tagcagatac taaaaaatca gatattttga aaacatttga tttggatcca gaaaagaaaa
     7561 cagtcttgat ttttggcggt agtcaaggag cacttaaaat caatcaagca gtatcagttt
     7621 ttttaagtga atatattcct tcttcttacc aagttttgta tgcttcagga gatagatatt
     7681 atgaagagat cctaaataaa ataaatcctg aaatacaaaa tattagtgtc cagccttata
     7741 tcaaaaatat ggcagaggtc atggcttcca gtgatctgct ggtggggcgt gctggtgcaa
     7801 cttcgatcgc agaattcaca gctctggggt taccggcagt gttggttccc agcccatatg
     7861 ttacgaatga tcatcagact aaaaatgcaa tgagtcttgt tcacgcagga gcagcaaaaa
     7921 tgattgcaga taatgaacta acaggtgaat cactaagtca gacagtcaac gaaatcatgg
     7981 gtgatgaaga attgcaaaag caaatgtgcc gagcttctaa agaacaagga atccctgatg
     8041 cttcaaaacg tctttatgac ttagtaaaac aaataatcaa aaaataaagg cggtggtctt
     8101 agtgagtaag aaaaaagaac caaaaaaatt gtctcatccg tcttctgatg aaaacaattt
     8161 gactccttgg caaaaggcta accgaaaata tctggcagaa caagagatga aaaaccaaga
     8221 gccaaaaaaa gaagacataa cattagatac acccctttca gattctgaag aagaaaaaac
     8281 ggttcaagag gaacagcaag aagaaagcca gtccgagaaa aaaatcgaat tttttgaaga
     8341 gatcaagcaa aagaaaaacg gtggaccata taacggttct tttttgaacc ggctgcccaa
     8401 tttaaaaagt caacggcaca aagtactatt tcgtcggtta gctttgatca tcactgtatt
     8461 gggtataccg ctgatttttt tgatctatta tgtttctcct tatagcaaat tacaagcagt
     8521 tgcagtatca ggcaataaga cagtcaacag ccaagaaatc atctctgata caaaattgtc
     8581 attaggagaa aatgtttggg gacagtattt tcatcgatct acttatatcg atcgccttaa
     8641 aaaagcacag ccaagaatcg aaacagcaaa tatacatttc aaagggatga atgaattcga
     8701 tttggatgta acagaatata aagagattgc tttgatcgca aaaaacaatc aatactaccc
     8761 agtgattgaa aatggaacag tattagatga aaaagtagca aatcctacaa aaaatctccc
     8821 tatcttggaa gagttcaaag actctgcaaa aatcaaagaa ttaacaaagc aatacaatca
     8881 gttatcatca gaattacaaa aagcaatttc tgaaatcaag tacacgccaa gagcaagcaa
     8941 taaaaatctg atccaattga atatgaatga cggcaatcaa gttattgtaa atatcaataa
     9001 tcttgcgaac caaatgaaat attattcgca agtagccaaa gatatggatg aaaaaggtgt
     9061 gatcgacatg gaagttggta tcttctcgca tccttacggt agcactcaag aaacggaaga
     9121 atcgggccaa tcggaagaaa caactgaaag tgaagaaaat gcggatcaaa caaacgaatc
     9181 aagtgaagag gcagaaacgc aaaaagcaca aacacctgga tcgtctagca caaacggatc
     9241 atctgattca aatgattcaa gtgaaacaag tcaaatagaa gataatccaa gtacaactcc
     9301 gtcaagttta aatgaaaatt gataaaacaa ctttaaaaag taagaatgta ctttctaaaa
     9361 accagtaatt tatggtagaa ttggaagcag tgaatatatc tattaataag tatattttaa
     9421 aacggacgct agttgttcgc ttgtatatgg aatagtaagt aggagggaat cccattcatg
     9481 gcaaaaacag gaatgtatgt aggccttgat attggaacga catctgttaa agttgtcgtg
     9541 gctgaatata tcgacagcca aatgaatatt attggagtag gaaacgcaaa atcagaaggg
     9601 attaaccgag gcattatcgt tgatatcgat aaaacggtcc aagcgataca acgagcagta
     9661 cgacaagcag aagaaaaagc aggtatccag atcaaaggcg taagtgtcgg tttgccagca
     9721 aacatgcttg aagtagaaaa ttgccaggga atgattgcag ttaacggaga ttcaaaggaa
     9781 atcaccgatg aagatgtcag aaatgtggct tcagctgccc ttgttcgttc aatacctcct
     9841 gaacgtcaaa tcgtgtcaat attgccacaa gattttactg tcgatgggtt cgagggaatc
     9901 aaagatcctc gtggaatgat cggcgtaaga ttagaaatgt acggtctgtt gtttacagga
     9961 cctaaaacga tcatccataa catacgtaaa tgcgtggaaa atgccggatt aatcgtcaac
    10021 gaaatggtga ttactccttt agcgctgact gaatcaattc tttctgacgg agaaaaagat
    10081 tttggtacga tcgtcattga tatgggtggc ggccaaacaa caacggctgt tatgcatgat
    10141 aagcaattga agtttaccaa tcttgaccaa gaaggtggcg aatttgtcac taaagatatc
    10201 tctattgtat taaatacgtc atttaacaat gcagaagcgt tgaaaataaa ctacggggat
    10261 gcatacccag aacggacatc accagatgaa gaatttccag tagatgtgat tggtcagtca
    10321 gaaccagtga aagtcgatga acgctacttg tcagaaatta tttcagctcg aatggaacag
    10381 attttcaaca aagcaaaaga tgcattggac caaatcgaag cattagagtt gccaggtggc
    10441 gtcgtattga caggcggagc tgctagtctt cctggtgttg tggacttggc acaagaaatc
    10501 ttcggcgtaa atgtaaaatt gtatgttcca aatcacatgg ggctgagaaa cccggtattt
    10561 acaaatgtaa taagtatcgt ggattattca gcaaacttga gtgaagtgta tcaattggca
    10621 aaaattgctg ttacgggtga aacctcagca gctcgtcaaa tggtagttga acaagaaaca
    10681 acaaacacat atgagagcta tgaagcacct gaagaaacaa tctacgatga gccggaacca
    10741 aaagagtccg gagaaaatgt aaaaaataaa atcaagggtt tctttacaaa tatttttgat
    10801 taattcatcc tgggaggaat aagatatgga attctcaatt gataataata tcaatgacgg
    10861 cgcagtaatc aaagtcatcg gagtcggtgg tggcggtgga aatgctgtaa accgaatgat
    10921 tgaagaaaac gttaaaggtg ttgaattcat tacagccaac acagacgtgc aagcattaaa
    10981 aaattcaaaa gctgaaacag ttatccaatt aggccccaaa tatactcgtg gtttaggtgc
    11041 tggttctcag ccagaagttg gacaaaaagc ggcagaagaa agtgaacaat ccttacgcga
    11101 agctttagac ggtgcagata tgattttcat cactgcagga atgggtggag gaaccggtac
    11161 aggtgctgca ccaatcgttg caggtatcgc gaaagaattg ggcgcattga ctgttggtgt
    11221 cgtgacacgt ccattcactt ttgaaggacc gaaacgtggc cgttttgctg cagaaggtat
    11281 cgctcgtttg aaagaaaatg tggatacatt attaattatc tcaaataacc gtttattaga
    11341 agttgtagat aaaaagacac cgatgcttga agctttccgt gaagcagata atgttttacg
    11401 acaaggtgta caaggtatct ctgatttgat cacagcgcca ggttacgtta accttgactt
    11461 tgctgacgtg aaaacagtga tggaaaacca aggtacagca ttgatgggga tcggtgtggc
    11521 tagtggagaa gatcgcgtag tagaagcaac gaaaaaagca atctcctctc cattgcttga
    11581 aacatctatt gatggagctg aacaagtact tcttaacatc acaggcggtt tggatatgac
    11641 attgttcgaa gcacaagatg cttcagatat cgtagcaaat gcagcaactg gcgatgtaaa
    11701 tatcattcta ggtacatcta tcaatgaaga aatgggcgat gaaatccgtg tgacggtcat
    11761 cgcaacaggt atcgatgaat caaaaaagga acgcaagtct agccgaccag ctagacaagc
    11821 acaaatgcaa tcacctgctc aaaagactgt attagatatg gatcaagcta aaccaacttc
    11881 ttcagaagaa gaaaacagtt ttggcgattg ggatatcaga cgtgaacaaa atgttcgtcc
    11941 tagagttgat gattccaatt ttgacaatat cgaaaaaaaa gaatttgata cattcaaccg
    12001 cgaagaaaca aaatctcatg gcgatgatga attaaatacg ccaccgtttt tccgtcgtaa
    12061 aaaataaagg agaggaagag cacatgattg ctgacaactt gcagaaaatc aagcaagaaa
    12121 ttcaggattc gtgtgctctt gttcatcgtg atccgaaaga agttacatta gtagctgtaa
    12181 caaaatctgt tgaatccgct caagctgaag aattggttag agctggcgtt atgaattgtg
    12241 cagaaaaccg agtagataaa ttattagaaa agaaacaaga tttatttgat tttcctgaaa
    12301 taaagtggca tttaattggt aatttacagc gcagaaaggt aaaattgatt gtaaatgaaa
    12361 ttgattactt tcatgcgttg gacagtttaa gattagcaga agaaatccag aagcggttag
    12421 agaaagaatt atcctgtttt attgaagtaa atgtttctgg tgaggaatca aaacatggaa
    12481 tttcaccaga tgagctttta ccgtttgtgg aatctttagc tgcctgcaca aaaatcaagg
    12541 ttgttgggtt gatgactatg gcgcctaaag aagcttctga tgagcaaatc cataaagtgt
    12601 ttggtacgct gaaagaattg cgtgatcttg ttcaagcaaa aggttatgcc catgcaccat
    12661 gtagtgaatt aagtatgggg atgagtcaag actttcaaat tgcgattgaa gaaggtgcaa
    12721 catttatccg tgtaggaaca gcgctattca aagacgaata aaggaggaac aaaaatgccg
    12781 cttttaaaca gaggagcaat tgctaatttt ttcggattag ctgatgagga agagtacgaa
    12841 taccaagatt atcctgaaac aacaaaaaag gctgtacagc caactaaaaa aaatacaaca
    12901 cagaatcaat caactcattc tgtatctgct gcgaatactc atgaaaaaca aaaaacggcg
    12961 acttcacgtc ctgtaacgca tgaaagtatt cagccggctg aaaagaaaaa agtaattgaa
    13021 ctacatcaat cacctacaaa aactgtagcg aaagaaagaa cagcttcaag aacctctaca
    13081 caacctaaag caaatcttgc tggaaaaatc actgtaatgg aaccaagagc ttattctgaa
    13141 gctatggcga ttgctaaacg aatcattgct ggagaagcaa ccttagtgaa ttttcattta
    13201 gtagaagaaa aacaagcgag aagaatcgtt gattttctaa caggaacagt ttatgcacta
    13261 gatggagata tcaaacgtgt tggagatgag atttttcttt gtacaccgac tgggatggag
    13321 attgacagtt caaccgctca atcttttgca gatgcgaatt tgtttgatct gtaagaggag
    13381 gaactacgat ttatactatt gcactattac ttcatttact tcaacgggca gcctatttct
    13441 atagcattat cttgatggta tatgcattgc tctcttggtt tccaggcggg tatcaatcag
    13501 ggctaggacg attattagct aagatctgcg aaccgtatat cagtttgttc gatcgtctgc
    13561 ctttgaggat tggacccttg gatttcacaa ttttaggggc aatcctgatc ttgaacttag
    13621 ccgtacaagg tttagcacag atcgttatat ggataactgt ccatttagtt tattgattga
    13681 agcaacaaaa tctttattct cagaatagat aggaggggaa aattttggat gcaaatgtat
    13741 accagcactt tcgggcagat gaacgtacat ttatcgattc ggtaggcgat tggattgaac
    13801 aggttcaagg acaatatgca ccctatctga ccgaatttct tgacccaaga caatcctata
    13861 ttttagaaac actgattcga caaagcagtg atttgcgttt catgttttat ggcggatatg
    13921 aacaggcaga aagaaaaaga tgtttgattt tccccgatta ttacgaacct gtagaggaag
    13981 attttgaaac agcattgtat gaaatccatt atcctagcaa atttgccagc ctaagtcatg
    14041 ggaaaatcct tggaacttta gtgggaacgg ggatcaaacg ctcttacttt ggcgatatca
    14101 tctcggacgg tgatcgctgg caagtattta ttgcaaatga ggtggatcat tttgttgctg
    14161 cacaagtaac aaagattgga aaggttgctg tacgcttgga accaatcaaa tacacagaaa
    14221 tgattatgcc gaaagatagc tggacacaag aaagaggaac agttagttcc atgcgactag
    14281 acagtgtgat ctctgagatt tataatattt ccagacagcg gtccaagcag ctgatcgaag
    14341 ctggtaaagt aaaagtgaac tggacagaaa atacaagacc tgattttgta cttgaactat
    14401 tagatattat ttcagtcaga ggttatggca gagtccagat ccaagaactt gaggggaaga
    14461 cgaaaaaaga aaagtacagg gtactgttcg gtgtattacg taaataaaat aaaaaggtgg
    14521 tttactcatg gctttaactc cattagatat ccaaaataag acattcccga caaaaatgcg
    14581 gggatataat caagacgaag tagatgattt cttagattta gttgttcgtg actatgagga
    14641 attgactcaa agaaatcgag aattagaaaa agcagtcaaa cattctgaag aaaaacttga
    14701 gtacttcaat gaattaaaag atgctttgaa ccaatcaatc atcgtagctc aagatacggc
    14761 tgataaagta aaaacaagtg caagcaagga atccgaagtg atcgttactt ctgcacaaaa
    14821 caaagcagat gagttagttg cgaacgctga aaaacgtgca catcaattaa caactgatgc
    14881 agaagaaaaa gcaagaaaaa tcttgacaga tgcaacagaa aaagcaagac agcttgcgac
    14941 agaaactgaa gatcttaaga aaaaaacacg agtattccat caacgtatca gcttgatgct
    15001 tgagtctcaa ttagaacaag tgaaaagtcc ggaatgggat gaaattttac aaccattctc
    15061 cagctacgtg actgacagcc atgaagtgat caaagaagtt ttagcaaaac agcttgacaa
    15121 tgaaaatgat actgatgtaa actcagacac aggagtcata gaagcaccta tgacggcatt
    15181 tgatacacag gctattgata tctcgatcgt gcctggtgaa aatgaaaacg agtagaacag
    15241 aaaagcactt tgttattctt aaagcgagcc gatgagagtg gaagttcggt aagacactgc
    15301 atggtgaaag atcctctact tgagacctgc ttttccgaaa ttagagtaag aagcagcggc
    15361 tgacctcgtt attggtcaca gagagtttgc actagataga tgcaactaaa tttgggtggt
    15421 accacggcat gttcgtccct tgcggctgaa catgcttttt tgtgtccgga aaacaaataa
    15481 aggagtgtat ggttgaatga aaatgaaaga aaccttgcaa ttaggaaaaa caaaattccc
    15541 tatgcgtggt aatttgccaa accgagaagg cgagtggcaa aaagaatggg aagaaaatcg
    15601 aatctaccaa aaaagacaag agttgaacga aggaaaacct agctttgttc ttcatgatgg
    15661 acctccatat gccaatggta atatccattt aggacattca ttgaataaaa tcagtaaaga
    15721 cattatcatt cgttcaaaat caatgtctgg ctatcgtgca ccatatgttc caggatggga
    15781 tacacatgga ttgccgattg aacaagtatt ggctaataaa ggcgtgaaac gtaaagaaat
    15841 gtccatggca gaatacttgg aaaaatgtcg tgaatatgca ctttcccaag tagataagca
    15901 acgagcagat ttcaaacgcc taggagttgc aggagattgg gaacatcctt atgttacatt
    15961 agatccttct tatgaagctg cacaagtacg cgtatttggt aaaatggctg aaaaaagcta
    16021 tatttacaaa ggattgaaac caatttactg gtctccgtca agtgagtctt ctttagcgga
    16081 agctgaaatt gaatataaag atgtaaaatc tccatctatt tttgtagctt tcaatgtcaa
    16141 agacggcaaa gatattcttg atgaagatac agcttttgtc atttggacga caacaccatg
    16201 gacattgcca gcaaaccaag gtattgctgt aaatccaaaa tttacgtatg tattggtgga
    16261 agccgatggc cgtaagtttg tcgtagcgaa agatttgcta gaaacagtcc aatcagaaat
    16321 cggttgggaa aatgtaacag ttctaaaaga atttgctgga caagaaatgg aatatatgac
    16381 tgcaagtcat ccattctatg aacgtgaatc acttgtgatt ttaggcgatc atgttacttt
    16441 agacgctggt actggattag tccatactgc tccaggacat ggggaagatg actatatcgc
    16501 tggtagcaaa tataaactat ctgtagtgtc acctgtagac tcaaaaggat tgtttacaga
    16561 agaagcacct ggatttgaag gtatctttta tgacaaagca aatcctatga tcactgatct
    16621 attaaaagaa aaaggagctt tgctgaagct agatttcttt actcatagtt atcctcacga
    16681 ttggcggacg aaaaagcctg tgatctatcg ggcaactcca caatggtttg catctatcga
    16741 taaattccgt cagaatattt tggatgaagt agaaaaagta gagtgggtta tcccatgggg
    16801 taaaacacgt ttgtataata tgatccgtga ccgcggcgat tgggtaattt cccgtcagcg
    16861 tgcatgggga gtaccacttc cgattttcta tgctgaaaat ggagaagcaa tcatcacacc
    16921 agaaacaatc gaacatgtgg caaaattatt tgctgaacat ggatcaatta tctggtttga
    16981 aaaagaagcg aaagaacttc ttcctgaagg atttacccac ccaggttctc ctaatggcga
    17041 atttacaaaa gaaaaagata tcatggatgt ctggtttgat tctggttctt ctcatgaagc
    17101 tgttttacgt cagcgaccag aattatcatt cccagcagat atgtatttag aaggatcaga
    17161 ccaatatcgt ggctggttca actcaagtat cacgactagt gtggctatca atggtgtagc
    17221 accatacaaa tcagttattt ctcaaggatt tgctttagat ggtgaaggcc gtaagatgag
    17281 taaatcttta ggaaacatca ttgcaccaga aaaagtgatc aaacaatttg gtgcagatat
    17341 ccttcgctta tgggtatcta gtgtagatta tgaagcagat gttcgtgttt ctatggatat
    17401 cttaagtcaa gtggcagaag tatatcgtaa aatcagaaat acgatgcgtt tcttattagc
    17461 gaatacagaa gatttcgatc ctaaaaaaga tactgtttcg tacaatgatt tacgttctgt
    17521 agacaaatat atgaccgttc gattgaacca agtaatcaaa gaaatccgag aagaagggta
    17581 cgacaaatat aacttcatgc atatttaccg tacagttatg aacttcttga cggttgatct
    17641 atcgtcattc tacctggatt ttgctaaaga tgtagtttat attgaagcag aagatgatta
    17701 tcaacgtcgt tgcatgcaaa ctgttttcta tcaaacagct gtagctttga caaaattatt
    17761 gactcctatc attccgcata cagcagaaga aatctggtca ttcctgaaag aagaagagga
    17821 gtacgttcag ttaagcgagt ttcctggtta tgaagagttt gcaaaccaag aagaattgat
    17881 ggatacctgg actgctttca tggacttccg tgataatgtc ttaaaagcat tggaagaagc
    17941 aagaaacagc aaattgattg gtaaatcttt ggaagcaaaa ctaactgttt atccaaaaca
    18001 acaagttcgt gaaatgttaa aagctttgga tgcagatatt gcacaattat tgatcgtttc
    18061 accagactac tttgaagtta tggcagcaga tgaacaagtg ccagataacg ctatggtatt
    18121 tgatgatgtt gctattacag tggaaaaagc agacggggaa acttgcgatc gctgtcgcca
    18181 agttcgtaaa gatgtaggag tagatgaaaa acttcctcat ctatgcggtc gttgtgcaaa
    18241 aatcgttgaa gacttctatc ctgaagctgt agctgaagga ttcgaagaaa agtaaaaggg
    18301 aaaagggggt gtgacatgtt ctttgtcaca cccccttttg ttatcaatcg tttaattttc
    18361 cacgttcacg ataccagtta tctggttgcc agatccattc gaatccgtct ttttcaagta
    18421 gttcgaaagc tgctttaggt cccattgatc cagctttgta gttgggaaaa tccggttgtg
    18481 tttggtccca agcttttcga atgacatcca cgatatgcca agattgagcg acttcttccc
    18541 aatgagagaa gttcgttcca tcaccattca acgcatctaa tagtaatttt tcgtaagctt
    18601 ctgggctatt ttcaaccatt tctgcactat ttctaaattc caatttgatt ggttccgttt
    18661 caaatccttg ccctacttct ttcccattca aggataaaga aaatccttca gttggctgaa
    18721 tatagatcgt tagaacatta ggtggaagtg tcgtatcgtc acatggttca tcgacagatg
    18781 ttttgaatac attaacagga acttgtttga atacgatgtt gatccgagtc cctttttcag
    18841 tcaaacgttt gcctgtacgt acatagaaag gaacacctga ccaacggaag ttatcgatag
    18901 tgaatttgcc tgcgacgaat gtttcagtct ctgattgagc atccactttg tcttcttcgc
    18961 gatagccctt gaattgtaca ccatccaatt caccttgagc atattgcccg cgaacgaagt
    19021 tttctaacac ttcttcttcg ttataaatgc ggatagcttt caatgctttg atcttttctg
    19081 tacggatttc tttttcggaa aatgctactg gcggttccat tgcaagtaat gctacaacct
    19141 gtaaaatatg attttgcacc atgtctttaa gtgcgccact atgatcgtag tagcccccgc
    19201 gatcttctac accgaggttt tcgccaaaag taatttggat attgtcgata tagcgattgt
    19261 tccattgagc ctcaaaaata ttattggcaa aacggatagc tgagatgttt tggatcattt
    19321 cttttcctaa ataatgatca atcctgaaaa tatcttgttc tgggaatact tctctgattt
    19381 gctggttcaa ttcaaaagca gactggtaat cagaaccgaa cggtttttct atgatcaaac
    19441 gattgaagcc attttttgtc ataatatttt gtgatttcaa atgagaaacg atcgttccaa
    19501 aaaattgagg tgccatagcc aaataataga cacggttacc gtttaattga tagtgtccat
    19561 ctaatcggtc agacaattct tttaatgtct gataatgcgt tgaatcattt acatcatggg
    19621 attgatagta aaaatggctt gcaaaggtag ttgcttcttc atctgaagga ttcaatgcac
    19681 ggattgtttc tttgacaact tcacggtagt gctcatcgct ccagggacgg cgagcggttc
    19741 cgattacagc aaaatgctcg cctaaatttc cttttctgta aaggcggaac aaagacgggt
    19801 acaatttacg ctgggctagg tcacctgtac cgccgaatat cgtaaacaat acattttttt
    19861 cattagacat gtggatacac tcctttatat tttatcgaca aaaatcgtgc tggccgcctt
    19921 aaagctgacg gggattttct ttctatcatt ttggatagtg atcggtcctt cataagctgc
    19981 gatgtcaacg atctcatatt cttcgttgat attcaggtca atagtgacta agtaatccaa
    20041 caattctttt tcatcaagca ctcgtgcgat acgcactttt gtaccgacag gataggactc
    20101 caatgtttgt cggcgttctt catggacaac ttcctctttt ttaggaatcg atcctccatg
    20161 aggacaatgt tcaggatgat tcaaatagtt ttctaaatga tctgcaagat gttccgaagt
    20221 cacatgttcc aatacttcag catcatcatg gacttcattc cagctatagt ctaaatgttc
    20281 aactaaaaaa acttcccata agcgatgttt gcgaatcaga ctgctagctt tttgcagtcc
    20341 gcttgcagtc aattgtacac cttgataagg ggaatgttct acaagctctt ctttcacaag
    20401 tttagaaatc atttcactga cagatgcagc tgagacatct agactagata cgatttgttt
    20461 attgttgact ttcgttgtat ctccacctag ttctaaaatg attttcaaat aatcttctcg
    20521 atttggtgtc atattcgatc atcctttcat ggtaaaaaac catcattagt aagtgtaaca
    20581 aaaaaaacgt taattgacta tttttctaaa taaaaaacaa aggattttta gacgttttta
    20641 caacaaaaaa cgaaagatga tagagaagac tcattctttc gtttataaag gatatatata
    20701 tacattggga gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc
    20761 ataaagagtt tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac
    20821 taaacgatat tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa
    20881 cgtgtaatca gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt
    20941 accttgatac tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat
    21001 aaaacttgag agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa
    21061 atcactcctt tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta
    21121 ccgaataaaa gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca
    21181 aattatgata ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg
    21241 aaatagcttt ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg
    21301 gagaaataat tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag
    21361 gcgctgaagt ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga
    21421 ttgctgtaaa aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg
    21481 caattgatgg tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg
    21541 tatacaagcg tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg
    21601 tcggacaaaa agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg
    21661 cttttcctga tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg
    21721 cacaagatgg ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg
    21781 caacgatcca tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa
    21841 gcggagttca aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc
    21901 aggccattcc atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta
    21961 aaataatgat caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag
    22021 cagctttaag cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag
    22081 cacaggaatt tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt
    22141 ttttcaatat tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta
    22201 gtcaagctaa gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt
    22261 cgacgccaaa agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt
    22321 ttgaagcagc aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat
    22381 taaaagacga gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc
    22441 aagtattaga tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga
    22501 tggatttagg tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag
    22561 tccattattt cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac
    22621 gttatgagaa ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt
    22681 cagagattta ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag
    22741 cacaatttca tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa
    22801 atccaatggc agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag
    22861 cttggggatt gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt
    22921 atgatttaag aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat
    22981 gaacgaattt gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt
    23041 tatctcacct tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat
    23101 agtagcggtt tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact
    23161 aaacgtggaa ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc
    23221 tattcaagta gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc
    23281 tttggtgggc ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat
    23341 cttgattggt acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca
    23401 tcaagtgaaa acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca
    23461 aatcattgat caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc
    23521 gacagcagat cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat
    23581 tgacgtgaca gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc
    23641 gatacgaaag aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct
    23701 gattttcttt aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg
    23761 tgtaccggta ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga
    23821 acaattcaaa aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt
    23881 agatattaca ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta
    23941 tttgcatcgt tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt
    24001 acaagataat aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga
    24061 agagattttt ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc
    24121 tcctgcagta aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata
    24181 taacaaagaa aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg
    24241 agcaaggcgc aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt
    24301 tgccagagga ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa
    24361 aaccaacaag gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat
    24421 gcaagccgat atttaagtga actataccag gcccatcata ttgtaaaaac cgctggccga
    24481 ccagtcgttt actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct
    24541 tctacgcaag tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata
    24601 cagcaggcaa aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga
    24661 gaaactggta cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca
    24721 gagatgcttt ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgctcaaaat
    24781 ccgcaactgc tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct
    24841 caagatagtc ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc
    24901 caccgtttac cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac
    24961 cgtccgcttg gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg
    25021 actgaatctt cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc
    25081 ttgccgaatc ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc
    25141 aaacaagaag cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc
    25201 ttcatgcttt atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc
    25261 tgtgcaaaat cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa
    25321 gaagaacttt cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt
    25381 ctcgaccgat tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat
    25441 gttgtctggt cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa
    25501 gtcacttctc tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa
    25561 gatgtggatg cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa
    25621 gatcttttgc cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa
    25681 cggttagacc gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt
    25741 acattggatc gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg
    25801 aaaaatttta agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa
    25861 gaacaagttg agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat
    25921 ctaggagaaa gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc
    25981 agacagactg cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg
    26041 aaagctttta atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa
    26101 tatgtcaaaa actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga
    26161 tcattaaaca ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc
    26221 acgatgacaa gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc
    26281 ttagaagata tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc
    26341 aaagcggaaa agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact
    26401 ggtgaaggag tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag
    26461 gtagaactga tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc
    26521 ttattggaag aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat
    26581 atcccatttt tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt
    26641 atagctgatg atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc
    26701 gatgtctttt ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag
    26761 accgatttgt cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc
    26821 ttcatgttag acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt
    26881 ttctcaaaaa tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa
    26941 cgaaaatatc aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt
    27001 gaaaataaaa taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt
    27061 gcgcactata aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg
    27121 gcatgcttct tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa
    27181 aaaaacaatc atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat
    27241 gcaaaaagca gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga
    27301 tgcagataat aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg
    27361 tttcatgaaa gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat
    27421 caatatgcaa gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct
    27481 aatgaaataa atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta
    27541 gaagccatca tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg
    27601 attgcggctg ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa
    27661 gaatcactta tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga
    27721 aatcatatgc aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt
    27781 gcgtttactg atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa
    27841 atacaacttc ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta
    27901 taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat
    27961 aaagtttgat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt
    28021 tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg
    28081 ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg
    28141 acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg
    28201 gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt
    28261 ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta
    28321 ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc
    28381 cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca
    28441 cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt
    28501 ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca
    28561 acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca
    28621 ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg
    28681 tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag
    28741 gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt
    28801 tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag
    28861 cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag
    28921 ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg
    28981 cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt
    29041 tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca
    29101 atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc
    29161 cattcttgat tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc
    29221 tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct
    29281 tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt
    29341 tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac
    29401 aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt
    29461 cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa
    29521 ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc
    29581 aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa
    29641 ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa
    29701 atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt
    29761 ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt
    29821 atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa
    29881 agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag
    29941 catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg
    30001 gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa
    30061 ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta
    30121 catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga
    30181 cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg
    30241 atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag
    30301 atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat
    30361 gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag
    30421 cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc
    30481 gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag
    30541 gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga
    30601 ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga
    30661 gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg
    30721 gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc
    30781 ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga
    30841 taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa
    30901 aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat
    30961 gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa
    31021 gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata
    31081 tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca
    31141 tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt
    31201 tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc
    31261 ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga
    31321 aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta
    31381 tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc
    31441 aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct
    31501 attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa
    31561 agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta
    31621 tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg
    31681 gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt
    31741 tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc
    31801 cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc
    31861 attggaaagg acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac
    31921 tagtggaaac gttttgccta acttataagc tgattttttt agcagataag cttgcaagta
    31981 aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag
    32041 caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc
    32101 cctgtatcct ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta
    32161 gttatcattt ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc
    32221 aagctctcga acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa
    32281 acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg
    32341 ccttagtcca tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa
    32401 atatttgctg acggcgtttg atgaaa
//
LOCUS       DACESX010000016        30818 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.016, whole genome shotgun sequence.
ACCESSION   DACESX010000016 DACESX010000000
VERSION     DACESX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.016"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            623..1951
                     /locus_tag="IXF88_000650"
     CDS             623..1951
                     /locus_tag="IXF88_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1507399.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2153..2986
                     /locus_tag="IXF88_000651"
     CDS             2153..2986
                     /locus_tag="IXF88_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1507400.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3002..3739
                     /locus_tag="IXF88_000652"
     CDS             3002..3739
                     /locus_tag="IXF88_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1507401.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3894..4862)
                     /locus_tag="IXF88_000653"
     CDS             complement(3894..4862)
                     /locus_tag="IXF88_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1507402.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5094..5927
                     /locus_tag="IXF88_000654"
     CDS             5094..5927
                     /locus_tag="IXF88_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1507403.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVFKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5961..6800
                     /locus_tag="IXF88_000655"
     CDS             5961..6800
                     /locus_tag="IXF88_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1507404.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6826..7386
                     /locus_tag="IXF88_000656"
     CDS             6826..7386
                     /locus_tag="IXF88_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1507405.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7585..9306
                     /locus_tag="IXF88_000657"
     CDS             7585..9306
                     /locus_tag="IXF88_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1507406.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9471..10613
                     /locus_tag="IXF88_000658"
     CDS             9471..10613
                     /locus_tag="IXF88_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1507407.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10629..11840
                     /gene="thiI"
                     /locus_tag="IXF88_000659"
     CDS             10629..11840
                     /gene="thiI"
                     /locus_tag="IXF88_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1507408.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12413..13060
                     /locus_tag="IXF88_000660"
     CDS             12413..13060
                     /locus_tag="IXF88_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1507409.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            13453..16098
                     /locus_tag="IXF88_000661"
     CDS             13453..16098
                     /locus_tag="IXF88_000661"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1507410.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16408..17721
                     /locus_tag="IXF88_000662"
     CDS             16408..17721
                     /locus_tag="IXF88_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1507411.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17711..18364
                     /locus_tag="IXF88_000663"
     CDS             17711..18364
                     /locus_tag="IXF88_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1507412.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18419..19111
                     /locus_tag="IXF88_000664"
     CDS             18419..19111
                     /locus_tag="IXF88_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ1507413.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19261..19461
                     /locus_tag="IXF88_000665"
     CDS             19261..19461
                     /locus_tag="IXF88_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1507414.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19715..21394)
                     /locus_tag="IXF88_000666"
     CDS             complement(19715..21394)
                     /locus_tag="IXF88_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1507415.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21396..21608)
                     /locus_tag="IXF88_000667"
     CDS             complement(21396..21608)
                     /locus_tag="IXF88_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1507416.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            22001..23731
                     /gene="efrA"
                     /locus_tag="IXF88_000668"
     CDS             22001..23731
                     /gene="efrA"
                     /locus_tag="IXF88_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1507417.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23728..25488
                     /gene="efrB"
                     /locus_tag="IXF88_000669"
     CDS             23728..25488
                     /gene="efrB"
                     /locus_tag="IXF88_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1507418.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25583..25780
                     /locus_tag="IXF88_000670"
     CDS             25583..25780
                     /locus_tag="IXF88_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507419.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25933..26418
                     /locus_tag="IXF88_000671"
     CDS             25933..26418
                     /locus_tag="IXF88_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1507420.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26522..27115)
                     /gene="clpP"
                     /locus_tag="IXF88_000672"
     CDS             complement(26522..27115)
                     /gene="clpP"
                     /locus_tag="IXF88_000672"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1507421.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27295..27822
                     /locus_tag="IXF88_000673"
     CDS             27295..27822
                     /locus_tag="IXF88_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1507422.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27913..28650
                     /locus_tag="IXF88_000674"
     CDS             27913..28650
                     /locus_tag="IXF88_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1507423.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28654..29571
                     /gene="miaA"
                     /locus_tag="IXF88_000675"
     CDS             28654..29571
                     /gene="miaA"
                     /locus_tag="IXF88_000675"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1507424.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29573..30802
                     /gene="hflX"
                     /locus_tag="IXF88_000676"
     CDS             29573..30802
                     /gene="hflX"
                     /locus_tag="IXF88_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1507425.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10176 a         5273 c         6576 g         8793 t
ORIGIN      
        1 cgtaggttcg agtcctactg ccggagtaaa taaagcttat gcaaagaaaa agaaatccaa
       61 tcacttgggt ttctttttct tttattatta tgatgaatca agctttctgt ttttgtcacg
      121 tatgttgtaa tctttacgaa agagtagagt tggctactca taatcaacca tacttcccat
      181 catcgctaaa tacgaaataa atcagcattc ctacaccaat caaagcacaa gctgcaccaa
      241 gaaaagggat gacattccca aagttatctg tattcataag tatgccacca cccacaaaca
      301 aaaagccgcc agcaatcagg tttttcattt tattcatctc ctttcttacg ttatagtatt
      361 tccttctttc aatgagtatt ttgaaagcta tcttattgta tgatgttctt ctaggcctct
      421 tctttattat acaggccatt ttcgttatct catagaaata ttccttttta tctgaaaact
      481 attgtatacc gtatgacatt tttcttcatg aagttgtaag tatattctca ttcaaatgaa
      541 aatttcatca tagaagagat tgtatttcta aattcttaag agtaaaatac gaagtataac
      601 ttggatttgg aaggatgatt ggatgaaagc tattacgcct gatatgacaa caaaatttaa
      661 caaacagttt caagcaaata tcaaacagca ggcattgcag cgggcagttg tgaagaacgg
      721 aattactgct tctgctgaaa atgtttcagc acatgtggag aatacaccgg tattttctat
      781 agatttggct acggggaaag tggctaatca aatgcaatcg gggcgttgct ggatgtttgc
      841 tgcactgaac acattccgtc ataagatgct gaatcaattc catttaaaag agtttgaatt
      901 atcacaaaat tacacttttt tctgggataa atatgaaaag gcaaattatt tctatgaaaa
      961 catcattgca acaggaaaag aggcaatcga tagccggaaa gtagctttct tactcgctac
     1021 tcctcaacaa gatggcggcc aatgggatat gatcgtttca ttattccaaa aatatggcgt
     1081 tgtgcctaaa tcggtgatgc cggaaagtgc gaatagttca aattctcgag atttgaataa
     1141 ctacttgaac aaattactgc gtaaagatgc agtagtgtta cggaaaatgg ttgctcaagg
     1201 agaaacatta gacgctattg aagaaaagaa agaagaaatg ctagaaagca tttataactt
     1261 cttagctatc tcattaggaa cacctccaaa agaattcgat tttgagtacc gcgatgaaga
     1321 aaagaactat catcttgacc gaggactgac accacaaatt ttttatgata aatacatcgg
     1381 tgtaaaatta gatgattatg ttagcgtgat caatgcacca acaaaagaca aaccattcaa
     1441 tcgttcttat acagtagaga tgctaggaaa tgtcgttggc gggaaagaag tcaaatatct
     1501 gaatgttgat atggaaactt tcaaaaaatt agcgattgct cagcttgaag aaggagaatc
     1561 tgtttggttt ggttgtgatg taggtcaatc ttctactaga actagtggca tcatggcctt
     1621 agatgcttat tcaatggatg atctatttga tacggatttt acaatgacta aagctgaacg
     1681 tttagatttt ggagaaagtc tgatgaccca tgcgatggta ttgaccggtg ttgacttagt
     1741 agacgggcaa tcaacaaaat ggaaagtcga aaatagctgg ggcgaaaaag ttggaaagaa
     1801 tggcttcttt gttatgagtg atgcctggat ggacgaatat acgtaccaaa tcgttgtaag
     1861 aaaagattta ttgacgaaag agcaattaaa tgcgttcaat gaagaaccaa ttgttctatc
     1921 tccgtgggat ccaatgggtg ctttagcata gacgagtatt tcaatgaaga gtatgtagta
     1981 aagataaaaa gagctggctt aagccagctc tttttatctt taaagtccat tccctgtttt
     2041 ttatggtccc tgtgctataa ttctaaataa aggataagga ttggaaataa tccttttatc
     2101 attaaaaata tgatgacgaa gtagaataaa attgggagta ggggggaaag tcatggatat
     2161 ccaaactgtt ttatctgatc tgaaattaaa tggtaaagtg attccagttg ttggtggcga
     2221 tgttaatcag acctatcgta taaaaaccga acatcgtgct tattttttaa aaatccatcc
     2281 gaatgtaaaa aaaggatttt ttgaagcaga agtagacgga ttaaaagaac tgtcagcatt
     2341 cgtacgtgta ccggatacgt atatgctagg tgaaacaagt gaaggcgctt atttgttaat
     2401 ggaatggatc gagccgggaa aaggggatca acgagatctt gctgcagctt tagctaatct
     2461 ccatcagcaa acagcgccac aatttggttt tcgtaaagat aactatttgg gtacacttgt
     2521 tcagaaaaat tcttttgaag aagattggtg gacttttttc tttaaagatc ggcttgaatc
     2581 ccagatttct ttggcagaag aaacgaatcg atggaatgta cagcgtcaag aaaagtatct
     2641 gcgatttaaa gaacgtgtat tgaaatcggt tgaaccaaaa aagatcactc cacgcctctt
     2701 gcacggagac ctgtggagtg gtaatgtgtt ctttgaccag caagggcatc cagtttttgt
     2761 cgacccagct gtttcttatg ggaatcggga acaagatatt gcaatgagcc agttattcgg
     2821 tggtttccga ccggaatttt tagatgctta tcaaactata tttccattag aaaagggatg
     2881 gaaagaccga ctgccgattt accagttata ttatcttttg gcacatttga atatgtttgg
     2941 agaaagttac ggttcccaag tagatcaatt actagaaaat ttttaagagg agggcgtgtt
     3001 tttggcaaaa aaatatacca aaaagcatga agtttcatat tatgagtgtg acatcaacca
     3061 aacgatgaca tttccttcga tgttaggagt agttatcaaa acgtctgaag atcaaagcga
     3121 ccagttggga agaggcagtg aattcgtcaa tagctttgga cttacttggg tcatcacgaa
     3181 ttattccatg gagattaatc gtctccctag agtcggtgaa gtaatctcag taacgactca
     3241 agccatggag tacaataagt ttttctgtta tcggaacttc tggatccatg atgaagatgg
     3301 gaaagaatta gtcaaaatag aatctgtttt cgtattgatg gattttgtga atcgaaaaat
     3361 gagcagtgtt aatgaagaga tcattgctcc ttttgagagt gagaagatca agaaaatcaa
     3421 gcgtcaggaa aaaattgaaa agattgattc gggcttcatg ttgccatatc gtgttcgttt
     3481 ttatgatatc gatagtaatc agcatgttaa taatgcgatg tattttaatt ggatcattga
     3541 cgtattagga tatgactttt tgactacgca tgttccagaa aaagtaatta ttcgatttga
     3601 taaagaagta gagtacggga atgagatcga aagccatttt gaacaaatcc ctatcgaaaa
     3661 tggtgtcaaa acaaaacatg aaatccgaat gcaagaacag ctgtattgtg aagcaaatat
     3721 tatatggaaa aataaataaa aaagaggttg tgacagaagg gggcaacttc aagaaataag
     3781 aagaaattta cgaaaattgt ttctcaaatt tttgtaaatt ttcgacttag ttttcgaaga
     3841 agctattttt gtcacagcct ctctttttaa gtttagctat tagataatga tgtttagttt
     3901 tggataaacg aaatcaattc ttcacccgac aaaccatagt ttacagcaat caatagtttt
     3961 aaacgtgctt tttgactatt aatactgtta cagaaaataa tccccatttt ttctagttct
     4021 tttcccccgc cttcataatc ataaacaggc tctgcgattc cattgaaaca acgagaaaca
     4081 agtactacag ggattttttg gacaagaagc ttttccaaag cagctaaggc ttgtggaggg
     4141 agattccccg cacctaaagc ttcgattacc aaaccgtcta ctttcgtatg ggcgatggct
     4201 tccaataagt ccccttgcat cccagcatat gctttaacga tcgggatagt cccatctaca
     4261 gcggaaatgt ccagtcgctt tgtttctaaa agttcctgca agaataaaat tcgattctta
     4321 gttactaagc cgatgggacc caatgtaggt gttcggaaag tagcaacatt tgtggtatgg
     4381 gtctttgtca cgtagcgtgc agaatggatc tcatcgttca tgaccaccaa aaccccctta
     4441 tctagcgctt cttcacaact tgcgacacga atcgcacttt caaaattata aaggccgtca
     4501 cttccaagtt cattacttga acgcattgcc ccagtcaaaa caattggtaa tttgttgcca
     4561 atcgttgtat caagaaaaaa agcagtttct tctaatgtat ctgtaccgtg tgtgatcacc
     4621 actccggata tacctgatgc ataagcggac aagatacgtt cttttaatac caacatgtga
     4681 gaaggcatga tgtgtggtga gggaagattg aagatatctt caactaataa ttctacatgc
     4741 ttggggattg tgatttctgc atctaataat gggtttgcca catcaggact aacaccgccc
     4801 gtgagcatgt tttctttcat ggcgatcgtt ccgccggtgt gtagtacaag tatttttttc
     4861 attttacaga cttccttacg aataattttg catagccgtt aaatcttatt gtatactata
     4921 taaaagagtt ttgctatcag tgaagaataa cgaatagcct aacttatcat aaaaaagctg
     4981 accttattaa aatggaaaag actcaacctt gacctcctaa tacaatggag tatctggttg
     5041 agtttctttt acaagaaagc agccaaggaa atcaatcacg aaggtggccg gaaatgatca
     5101 gaatcggatt aacatcgttt aaagaacatg acaaattaac tggtaaaaag aataacactc
     5161 tttatgaata tgcagggttt ctacccttag tggaattaga tactgcctat tatggaattc
     5221 cccgaaagtc ctcagttgag aattggataa gtgaagtacc tgaatcattt cgctttgtat
     5281 tcaaagtcta tcaaggaatc agcggtcaag gagactggaa gccttattat cagaccgaag
     5341 aagagatgat tgcgcatttt ttagatgtta tgtcgccaat ggtagatgct ggaaagatgt
     5401 tttgcttttt agtccaattc ccctcatcat tcaaatgtac gaaagaaaat gtccagtact
     5461 tgcgcaaact tagaaagtgg tttgagggtt atcctgtttc tatagaattg cgggattatt
     5521 cttggtacag cgatgaattt cttgaacgta caagaaactt tatgtctaga gccgcttttt
     5581 ctttagccat catcgatgag ccacagttgc tgaatacaac gattcctttt gatccatttg
     5641 tcaccaatcc gaactttgct ctttttcgat tccatggtag gaatgcgcaa ggatggcaag
     5701 caaatgataa agagtggcgt aaaaaaagaa cgttatatcg ttattcagaa aaagaactgc
     5761 aagaactagc acatgaagtc aaagtagttg attctaagac aaaagaggta ggtgtgattt
     5821 ttaataataa ttctggtggt gatgcggctg aaaatgcatt agcactgcaa aaaatattaa
     5881 atatcaagcc agatggactc aatccaaccc aaatggatct gttttaaggg cttattttat
     5941 tcaagaaagg ggcgaagtct atgatcaaag ctattttttt tgatattgac ggaacattgg
     6001 tcaccaatcg ttcaaaagcg ttggaatcaa caaaacaagc aattgaatat gctcggaaaa
     6061 acggcatttt atgcggtgta gcaacggggc gcagcccagt aaagatcaaa gaaatcattg
     6121 atgaattgga attagatatg tatgtcgtgt ataatggtca actggttttt actgcagatc
     6181 gcacgattat cgaccaccct tttgaacaaa aagtattgga acatattgtt gaatttgcag
     6241 atgagaacca tcgacaaatc gtttttggtg cgagaaatcg gttagatgga tcaacgacga
     6301 tgctattagg acaatcgatt tttatcaagc gactagtcag ctttttaccg cgtaaattcc
     6361 cggtacgtct gatgaaaaaa atcctccagg tttttagccc tcaccgacaa aaggatcgtt
     6421 atgaaacgtt agagatatta aaagaaccaa tttatcaatg tatattgctc agtccggagt
     6481 cagaacagaa aaagttagaa catcgctttc ccgagtgtac gtttcagcgt tcaaatacgt
     6541 atactgtaga tatcatccca aaaggtggtt caaaattact aggcatccaa gctttcgcaa
     6601 atgctgtcga aattgaaatg gaagaaatta tggcattcgg cgatcattac aatgatatcg
     6661 agatgctgaa aggtgtagga attggagtgg ccatggggaa tgctcagatc gaagtaaaac
     6721 atgctgcaga ctttgtgact caatcaaatg aagaagatgg gatatacgaa ggactgaaac
     6781 acttcggtct gatttattag tcaatgaaag aaggaaaaaa tcagtatgga agcagagaca
     6841 ccttttcaaa aagtagaaac attccacaaa acttttgatc caagaagacc agagataccc
     6901 acggctttta cagaaaatga agctcataca cgtgctgcgt tcaaaataga agaacttgtt
     6961 gaatttgttg cagctacttc acctaataaa gaacaactgc atgaagctgt tatgctgctg
     7021 cacgaagcat tagataaagc agaagaaaaa atacttggta aaacacagtg gggaagtgat
     7081 ccgctggttg aacaagtaga tgcactgaca gatatccttt actttactta tggttctttt
     7141 tcattactag gaatagtgcc tgatcgaata ttcgaaatcg tccatcaggc aaatatgggg
     7201 aaattatttc cagatggaaa acctcattat gaccctgtaa caaataaagt gttgaaacct
     7261 gaaaactggc agatggaata tgctccagaa cctaaaatcg cggcagaaat ccaacgtcag
     7321 attcaagaag cgattgctaa gaagaaatcc gctgatatag gcatgtgcac aaatgaaaaa
     7381 gagtgatcag agtttcacta tttttgttat ctttatacga tggaaaaaca aaagattttc
     7441 gtttaagtta gtgaatgcat gaaaatagta tgctataatc tagaatagat gtcttttgca
     7501 cgagagacgt attatttgga tttatgccaa gcagaacgcc atctgttgca taaaagatgc
     7561 atactgaaaa cggaggaacc ataaatggga aataatgtta tcatcggaat cgtcattgca
     7621 atcattatag tcgccattgt cgtctttttg gttagttact tgatgaagaa aaaaaaccaa
     7681 gatcgattgg atgccttaga aaaacgcaag gaagaattat ttgacttgcc agtgattgaa
     7741 gaagtcgatg atgttaaaaa aatgcatctg gttgggcaaa gccaaaatac ctttcgtgaa
     7801 tggagtcaaa aatggaatga catatctact tcctcgttcg ctgaactaga aagtcaaatt
     7861 ttcgaagtgg aaaacctcaa tgaaagtttt cggttcttaa aaggaaaaaa agcagttgtt
     7921 gaagcagaag ctacaatgga ccaaatggag aaacaagtga cggaaatccg tgaaggtcta
     7981 aaggaattgc gggaaacgga agaacgcaac tcactagctg ttcagcaggc acttgacgtc
     8041 tatgaagaac tgaaagaaaa agtcagagaa gacgagcatg aatacggacc agctcttttt
     8101 gaattgcaaa aacagatcaa agtgatcgaa aaagaattta cgcagtttgt gactttaaac
     8161 acttctggag accctgttga agcccgcgag gttttggata aagcagaaaa acatacgtat
     8221 gaagtggaag ctctgatgaa aaaaattcca ccgctgtatg aggagctcca taccactttc
     8281 cctgaacaac ttgaagaaat cagtgatact tacgagaaat tgatggaaga acaatatgta
     8341 ttcccagaag aagatcttgc tgaagatatc gcaaaagtaa gtcgaagaat tgaaaattca
     8401 ttagccaatc ttgaaaaaac agaagttgaa acggtcgaat ttgaaaaccg tgaaacagct
     8461 gatttgattg attcgttata tgatatctta gaacgggaaa tggaagctca gagatatgta
     8521 aaaactaacc aatcaacgat tgcagagtat atcaaacata caacaaagaa taaccgtcaa
     8581 ttattgattg agctggatca cacatctcaa agctatacat tgaaccacaa tgaaattggc
     8641 cgtgtacgag gtttccaaac cgaagtggaa gaaatggaac gtcaaaatga acagatgatt
     8701 ccacagatcc gtcaacacga aattccttat tcagaagtgc gcacgttcta taaaacagtc
     8761 ttcaaagtct tagaagatat cgaaaagcaa caagtagaga tcgacgactc attacatgaa
     8821 ttgagaaaag gcgaaaaaga agcgcaagaa aagatcgata cattcgaatt caaattgcgc
     8881 agtttgaaac gttttgtaga aaaacaacgt ttgcctggct tgccgaatga ttacctagaa
     8941 ttcttttttg ttgctactga ccgtattgaa gaactaagtg ttgtcttgaa taaaattcga
     9001 gtaaacatgg aagaggtcaa tcgtttagtt gctctatgtg aagaagatct agaattatta
     9061 gataagaaga cacatgattt agtagatgca gcagcattaa cggagcaaat gctccaatat
     9121 gcaaatcgtt atcgtcatac acacgaagaa gtgagagaag caattgataa aagctactat
     9181 ttattcaata aagaatatca ctatcaagaa gcattagacg aaatcggtac agcattagag
     9241 cgagtagaac ctggtgcttt caaacgcatc gaagattttt attttaatca tccagactta
     9301 gtataaagca aaaaagttag taaaccagca agagcaagac ggattattgt cttgttcttg
     9361 tctttttttg tatatttgaa aattgaaaca agtgaaaatt tcacctgctc ttgaaagcac
     9421 cttggacaac cgttataata gaaaagaatt tcaaaaaaga ggggtttctt atgatatatt
     9481 ttgataacag tgcaacaacc ccaatctttc cgcaaagctt agatgcatat gtaaaaacca
     9541 gtcagcggat tattgggaat ccatcaagct tgcatgattt aggcaatcaa gctaaccggt
     9601 tgttgcagca agcaagaaag cagatcgctg atttgatcca tgtagaacca gaagaaatct
     9661 attttacttc aggcggaaca gaaggagata actgggtatt aaaaggaacg atgatcgaaa
     9721 aaagagaata tgggaatcat atgatcattt caggagtcga acatccggcc gtcagcgaga
     9781 cagcagaaca attaaaagaa ttaggaatcg aagtaagtat cgccccagtg gataaacgcg
     9841 gatttgtacg tgtcgacgaa ttgaaagaat taataagaaa agagacagta ttagtctctg
     9901 ttatggctgt gaataatgaa gtaggatcga ttcagcccat ccaggaaatc agtgatcttt
     9961 tggatgattt tcctaaaatt catttccatg tagatgctgt tcaggcgatt ggaaaagtac
    10021 cattctcgca atggttgaca gaaagagttg attttgcaac attttcagct cataaattcc
    10081 atggcccacg gggaactggt ttcatttatt ggaaaaaggg tcgtcgttta gcaccattac
    10141 tgacaggtgg agggcaagaa agaaatcagc gaagtgggac agaaaatgtt ccaggtattg
    10201 tggctatggt taaagcttta cgtttatcat tagagataaa ggaacaaaaa cctgagcaga
    10261 cacaacgctt aaaagtagct ctaatcaatg cgttgaagac gtatgagaaa gtgacgattt
    10321 tttcagaagg aactgactat gcaccgcata ttttatgttt tgctctaaaa ggaattcgcg
    10381 gcgaagttct cgtccatgcc ttagaagaaa aacaaatatt tgtttctact actagtgcct
    10441 gctcctcacg taaacatatg gcaagcagta cattgcacgc aatgcatgta ccaaatgatc
    10501 tggctacatc tgctgttcga atcagcttag atgaaaacaa ttcgcttgca gaagtcgagc
    10561 aatttatgat tattttcaag cagctttata agaaattttc tgcaatcaat taaaagagga
    10621 gtgttcttgt gaaatacagt gaaattatgg tccgttatgg ggaactttcg acaaaaggaa
    10681 agaatcgaaa aagctttatc atgcaactag cacaaaatgt cagacaagct ttgaaagact
    10741 ttccagaaat caaaatccat gctgatcggg accggatgca tcttttattg aatggcgcag
    10801 acgggcagct agttattcca aaactagcaa aaatatttgg gatccaaaat ttctctccaa
    10861 gtattcgtgt agaaaaagat gtcgaagttt tgaaacaagc tgttcaagac atcatgaaag
    10921 aaatttatac tgggaaagaa acatttaaaa ttatcgctaa aagaagcgat caccaatttg
    10981 aattgacaag taatgaattg aatcaaacat taggtaatgc tgtttttgat attttcccgc
    11041 atatccaagt tcaaatgcgt caaccagata ttccattacg tgtagagatt cggcgtgatg
    11101 gcgcttatct atcgtatgaa acgatcaagg gagctggagg attgcccgta ggaacaagcg
    11161 gaaaaggaat gcttatgctt tctgggggga ttgattctcc tgtagcaggg tactttgcca
    11221 tgaagcgggg agtggaaatt gaagctgttc attttgctag tccgccgtat acaagtgaac
    11281 aagcactgca aaaagcaaaa gatctaacag ccaaactagc accttatgtg ggaacgatcc
    11341 attttatcga agtgccattt accgagattc aagaagaaat caaaaagtca gtgccacaag
    11401 gatattggat gacaattact cgtcgaatga tgttgcgttt gaccgatgaa attcgccgta
    11461 ttcgtcatgg tttagtcatt ttgaatggtg aatcattagg tcaagtggct tcacaaactt
    11521 tgcaaagtat ggtggctatc aatgaagtaa cgaatacgcc aatcattcgg cctgtagcga
    11581 caatggataa acttgaaatc attgaagtag cagaacagat cgatacattt aatctttcta
    11641 tccagccttt tgaagattgc tgtacgattt ttgctcctcc acagccaaaa acacgccctc
    11701 aactggaaaa ggtccatcaa tatgaagagc gtttagcaat agatgaaatg atccaacgag
    11761 cactagctgg cttgaagatg gaagagatac aagcagaatc gtctaatgaa aaaactgaag
    11821 aattttcaga attgctataa gaggttatga aaatagtgtt ttgaccagag tattagagaa
    11881 acaaaacgaa aaaatagctc catgtatttt accgtttttg aactaatgtc gaagattagc
    11941 tgattgaata ccatttatca agaagttatg gaaaagccgg ttctttttta ctaactgaaa
    12001 aataaaattt tatttagaga tcaagctaca gttgataaat gatataaaaa agagaatttc
    12061 ccatgaaata gcgaggttgt gacagaagtg ggcagcttca agaaataaga aggaatttac
    12121 gaaaattgct tttcaaattt ttgtgaaatt tcggcttatt tttcgaagaa gttacttctg
    12181 gaacaccgtg tatcagaggt tgcgaaaaag ctgccctgca tcaagtaata agaacagcca
    12241 aacaaaaata gttcctcata ttttacgttt ggttgagctt attaccgcag atgcaagctt
    12301 ttgaacaccg ttttatttgt ctcacaccct tttccttcct acttgtaacc ttgctcccgt
    12361 gttagaaaca tgttacacta aatatgtgaa attattaaca aggagcgtcg ttatgcgaga
    12421 aaaaacagat acactgccaa aagcaactgc gaaaagatta cctttatatt tacgctactt
    12481 gaagatgctg gatgactcag gtataagtag aatcaaatca aatgagttca gcgaaatcac
    12541 ccaaattcct tctgcaacga tccgcagaga cttctcgcaa ttaggcgagc taggcagaag
    12601 cggatatggc tatgatgtcc cttttcttat cgatgtattc aataatatcc ttaatactaa
    12661 agaagaaaaa cgaatcgcat tagtaggata tggaaatctt ggcaaagcat tgaaacacaa
    12721 caattttaga agaaatgaaa atctaaatat cgtgtgcgtc tttgataatg atccagcact
    12781 gatcaatcga gtaattgatg gagaaatgat ctatccgatt gatcgtttcg cggagatagc
    12841 caaagcaaaa aatgtgacag tagcaatctc tacggtacca agtaaatatt cacaatctgc
    12901 tattgatgaa attgtcaaag gaaatgtaac cgctatactt aattttgcgc ctgacagagt
    12961 aactgtccca gcttatgtca atgttcaata cattgatttg acaaccgagt tgcagacgct
    13021 tatttacttt gatgagaatt actcagaagt tttttcttag gaaaatttga gtaggcttat
    13081 tgatcttgcg agaactcgtc gtaattcttt caaaaatttt ttagtactaa atgaagtggt
    13141 tttttcacga ccacttgttg actaaaaaaa aaaaccattg tagaatgaag cataacgctg
    13201 aacaagagga gtaactggca cattcattct tagagagaga actatctgct gaaaggttct
    13261 caatgacaac cagtgaaggt agcttgggag tttctgtctt gaatggagta gagatagacg
    13321 agtcgtgatc gttaactcac ttgagaggca ataatgattg attattgcaa tttaggtggt
    13381 accgcgtaaa tcatacgtcc tagaatacga aagtatttta ggacgttttt tagtttaaag
    13441 gaggaaaaca gcatgacgga agaaaataac ctatcaacaa aatataatcc gcaagaagtt
    13501 gaagctggac gttatcagca ttggttagaa caagatttgt ttaaaccgag cggagataaa
    13561 aaagcaaaac cttattcaat cgtaatcccg cccccaaatg ttactggaaa acttcattta
    13621 gggcatgctt gggatacgac cttgcaagat atgctgattc gtcaaaagag aatgcaagga
    13681 tatgatactt tatggctgcc tggtatggac catgcaggaa tcgcgacaca agcaaaagta
    13741 gaagcgaagc ttagagagca agggatttct cgttatgatc taggtcgtga aaagttcatc
    13801 gatcaagtgt gggaatggaa agaagagtat gcgagccaca tccgcgaaca gtgggctaaa
    13861 atgggcttat ctttagatta cagtagggaa cgttttacat tagatgatgg attatctgaa
    13921 gcagtacgta aagtttttgt taccttatac gaaaaaggtt tgatttatcg tggtgaatat
    13981 atcatcaact gggatcctca agcacgaaca gctctatctg atatcgaagt tattcataaa
    14041 gatatcgaag gcgcctttta tcatatgagc tatgaactag cagatggatc aggcgtagta
    14101 gaaattgcga caacccgtcc agaaacgatg ttgggagata cggcgattgc tgttcatcca
    14161 gaagacgagc gttatcaagc gctcatcggc aaaaaagtga ttctaccttt agtcaataaa
    14221 caaatcccaa tcattgcaga tgaatatgta gatatggaat tcgggacagg tgtagtaaaa
    14281 atcactccag cacatgatcc aaacgacttt gaggtaggta atcgtcatga tcttccacgc
    14341 gtaaatgtta tggatgatga tggtacgatg aatgaactag caggaaaata tgctggaatg
    14401 gaacgttttg ctgctcgtaa agcaatcgtg caagatctga aagaaatcgg tcgattgatt
    14461 aaaatcgata cgatgatcca cagtgtgggt cattccgagc ggacaggtgt cgttgttgaa
    14521 cctcggctgt ctactcaatg gtttgtaaaa atggcgccgt tagcgaaaaa agccattgat
    14581 aaccaagaaa cagatcaagc agtggaattc ttccctccaa gattcaatca aacgttttta
    14641 cgttggatgg aaaatgtcca tgattgggta atctcacgac aattatggtg gggacatcaa
    14701 attcctgcat ggtatcacaa agaaacaggt gaaatgtatg tagggatgga agcaccagcg
    14761 gatagtgaaa actgggtaca agatcctgat gttttagaca cttggtttag ttcagctttg
    14821 tggcctttct caacaatggg ctggccagat acggaaagcg aagattataa gcgatacttc
    14881 cctactagca ctttagtaac cggttatgac atcattttct tctgggtaag cagaatgatt
    14941 ttccaaagct tggaatttac tggcgaacgt ccatttgaga atgtcttgat tcatggattg
    15001 attcgtgacg aacaagggcg gaagatgagt aaatcattag gtaatggaat cgatccgatg
    15061 gaagtaatcg aaaagtatgg agcagatgct ctccgctggt tcttatcgaa tggttctgca
    15121 ccaggacaag atgtccgctt cagctatgaa aaaatggatg cttcttggaa ctttatcaac
    15181 aaaatctgga atgcttcacg tttcgttatc atgaatatcg aaggaatgac cgtagaagag
    15241 atcgatttat ctggagaaaa atcagtggct gaccggtgga tccttactcg tttgaatgaa
    15301 acgatcgaac gagtaactga gctatttgat cgttttgaat ttggtgaagc aggtcgacaa
    15361 ctgtataact ttatctggga tgatttttgt gactggtaca tcgaaatgag taaagaagta
    15421 ctttatggag atgatgctgt ttcaaaacaa accacccgta gcatcttagt ccatacactt
    15481 gatcagatcc tacgtttatt gcatccaatc atgccattcg ttacagaaga aatatgggaa
    15541 catattcctc atcagggaaa ttctctagta gtggcagaat atcctgttgt acgtccagag
    15601 tttgatgatg aaacagcttc taaagggatg gaagtattga aagaattgat tcgagccgtt
    15661 cgaaatattc gttcagaagt gaatacacca ttgtctaaac caattacgtt gctaattaaa
    15721 acaaacgatg ctaaaatcga tcaattttta gttgaaaata caagctatat cgaacgcttc
    15781 tgtaatcccg aagagttgac gatatcaagt gagatcactg cgccagatct agcgatgtca
    15841 gctgttctga caggtgcaga aatcttcttg ccgttagcag gattgatcaa tattgaagaa
    15901 gaaatcaaac gattagaaaa agaactggaa aagtggacaa gcgaagtaaa acgtgtccaa
    15961 ggaaaacttt ctaatgaacg ttttgtttct aacgcgccgg aagaagtagt agaggcagag
    16021 cgtgcaaaag aaaaagatta tttagagaag caaacggctg tcaaagaacg aatcgaaagt
    16081 ttgcgtacta ttcaataaga atagaaaaag agagtgtgaa atcagctagc tattactaaa
    16141 agctagctga ttgaacatcg ttacaggaaa gagtttgagc ctcactctta gcttcgattc
    16201 ataaggagat gtgacataaa ttttgtcgca tctctttttt agtctactct tattatgtag
    16261 gagaaggagg gaggtggatc atgtatcaag agtcaataaa gtagttgata aaatcgttcc
    16321 tctctctgtt tcttttctct ttgtcatact tttatttttc ttatgtgttc tatttgttat
    16381 aataaaagag aaatggggga attgttgatg acgattgaag aagcaataga atggatccat
    16441 agtcgattac catttggttc acgtcctggc cttgaccgag tcgaagcttt attagaagtg
    16501 gtagggcatc cagagagaaa agtaaaaacg atccatatcg ctggaacaaa cggaaaaggt
    16561 tctactgtag cttatttgag aagcctatta gaagaggctg ggctgacagt aggtacattt
    16621 acttcacctt atatcgaaac gttcaatgaa cggatcgcaa tcaatggcaa aagcattcca
    16681 gacaatgatt tgatccagct tgtaaaaaaa tatcagccac tgatcaaaca acttgatcaa
    16741 tttgaagcag tagctggtat tactgaattc gagacattaa cagcaatggc atttgattat
    16801 tttttgatga agaaagtgga cgtagcgatt atcgaagttg gcttaggcgg cttattggat
    16861 tctaccaatg tagctgtacc aatgctgacg ggaataacaa cgattggact tgatcatacc
    16921 gaaatacttg gagatacaat tgaagaaatc gctgcacaaa aagctggaat catcaaacaa
    16981 ggcgttccag tagtaacagg aaatattgcc ccagaagctt ttactgtcat ccaacaaaca
    17041 gctaaagcaa aaaatgcacc gctttatgta tacggggaaa attataaaat aaactatctg
    17101 catcctgatg agatgtgggg agaagtattt gaatttttta atgaaatagg aaagatccct
    17161 caattaacca ctccgctttt agggcaacat caagctgaaa atgcaggagt agccatcgaa
    17221 ttgtattttg tttactgtca attggaaaag ctgcctttcc gagaaaaaga tgttttgaag
    17281 ggattgaaga aatctcgatg gccaggaaga atggagcgtt tgagtaacga gccattagtt
    17341 gttttagatg gtgcccataa tacgcatgca atgaaccggc ttgttgagaa tttgaaaaaa
    17401 gaattcaaag gatatcaaat caacattttg ttttccgcct tacgaactaa aaatgttaga
    17461 gaaatgctag aacgattgca gcaagtacca aatgtccata tttatttgac gacctttgaa
    17521 catccaaaag ccatcgaatt aagtgaagta tcagatttag caagtgagag agtatcaatc
    17581 gtttcattgt ggcaattcgg attagcagat atattggaaa agatgacaaa tgaagacgta
    17641 ttagttgtga ccggttcact ttatttcatt tcagaagttc gccatttatt aatacaatta
    17701 ggaggagatc atgaagtata atgcagtgat ttttgacatg gatggtttgc tgttcgacac
    17761 agagttagtg tattacgaag ccacacaagt agtagcagac aagatgggtt ttccttatga
    17821 taaagacctg tatttaaaat tcttaggtgt ttcagatgaa gaagtatggg cgaattatca
    17881 tatgattttc tcggaatttg gcaaaacaag tgtacaaaga ttcatagatg attcatacca
    17941 ggaaacgctt gaacgttttt cgcaaggagc ggtacaaatg aagcccggtg tgttggaact
    18001 cctctctttt ttagaagaac atcagattcc caaagttgtc gcttccagta accaacgagc
    18061 agtcattgaa cttttattga agaagaacaa tctgtttgaa cgatttgaga cgattgtttc
    18121 tgcagaaaat gttcaacgag caaaaccaga cccagaaatt ttcttgttag ctaatcagaa
    18181 gctaggtaca gcgaagaaag aaacattgat tttagaagat tcacaaaatg gtattttagc
    18241 agcttctgcg gctgagatcc cggtagtcat ggtaccagat ctgcttccac catcccaaga
    18301 attgatcaaa aaaacagctg gggttgtttc ttctttacat gaaatccctg cattgttaaa
    18361 ataaaaatcc atctcttttc gtatcttatt ttagataaga tagaaaggag atgttttttt
    18421 gggaaaacga ttgatcaaag aagtacctac tagctcttta ccaagggaac gtatggaaat
    18481 ttatggcgcg gaagctttat cggatcagga gcttttggca atcttactgc gtaccggtca
    18541 acacccttat agtgtgatgt cgattgctgg aaatctgtta aaaacgtttg gaggactggc
    18601 ctctttacga caggctactt tgcatgagtt agaagagatt cgagggatag gacgcgtgaa
    18661 agcaatcgaa atcaaagcct tgatcgaatt aggtcgacga attcagacgg atcatgaaag
    18721 agcggctcca ccggttcgta ccagctacga attggcccag caattgattc tggaaatgaa
    18781 agatcacaaa caagaacatt tggtttgtat ttatttggac acgaagaatc aagtgatctt
    18841 aaaaaaaaca gtgttcatcg gttcgctgaa tcagtcagtc gctcatccta gagaaatttt
    18901 ccattatgct gtacgttatt gtgcagcaag gatcgtttta gcgcataatc atccttctgg
    18961 taatgttatt ccatcgcagc aggatatgaa ttttaccaaa aggatacaaa agtgtggaga
    19021 aatgatgggg atcactgtac tggaccactt gatcatcgga cgcaaacgat attttagttt
    19081 gagagaagaa ggaatgatgg aagagaagta agttgctttc ttgcaaaaaa aatgaagcaa
    19141 gtgtacaatt atattgtaat tttgtttggg aatagaagtt gaaacatctg ttgcacctct
    19201 atcgtacacg agcaatgtta caatttaaat tagtgctttt ggcacgagga ggatattatt
    19261 atggaaaacg gaacagtaaa atggttcaac gctgaaaaag gatttggatt tatctcacgt
    19321 gaagatggaa gcgacgtatt cgttcacttc tcagctatcc aaggtgacgg attcaaaaca
    19381 ttagaagaag gtcaagcagt gactttcgac gttgaagaat cagaccgcgg cccacaagct
    19441 gcaaacgttg taaaagctta attttatctg taaatcaaat tgatggtaga catcaatttt
    19501 tacgagagaa caaagcgagt aacaaaaata cctgccacaa gagacaattt ctcttgtggc
    19561 aggtattttt ttgtatgaaa aagtaaataa atttaagagg ctatgaaaga gccaccttgt
    19621 atcaaggaat aagaacaaac aaaaatggct tctcatgttt ttcatttgtt ttctcttata
    19681 ttcaggacaa ggtgctgatt tcataacctc ttatttattc ttcatctggt gtcattacca
    19741 ttggcaaaat cattggatgt ctttctgtac gttcgaataa gaaaggctga agcgcgccag
    19801 tcatcgcttc tttcaattta tattctgaac agtcaggtcc attcaaacat tctcttaaag
    19861 cgtggaataa gattcgctga gcttcattga tcatttctcc tgattctcgc atgtagacaa
    19921 atccacgaga gagaatatct ggaccagcca agatttcttt tttcttgata tccactgtcg
    19981 cgacagctaa tacaagacct tcttctgaaa gcaggtgacg gtcacgtaaa acgacattac
    20041 caatatcacc gatcccgcta ccatcgatat acacatctcc agccacaaaa tgcccagcag
    20101 gacgcgcaga atcgctagtt aacgctaaaa catcgccatt ttccatgatg aagcagtttt
    20161 ctgcaggaac acctacatct tgtgctagag aagcatgtac tttcaacatg cggaattctc
    20221 cgtggacagg aataaaatat tttggcttca tcaaacgaag cattaatttt tgttcttctt
    20281 gtccaccgtg accggaagta tggatgttat tgatttttcc gtgaatcact tctgcaccag
    20341 cttcagacag caaattgatc aaatgattga cacttgttgt attaccagga ataggtgaac
    20401 ttgaaaagat cactgtatcg tctggctgga tttggatttg acggtgagta ccattcgcga
    20461 tgcggctcaa cgcggccatc gcttctcctt gagaacctgt acataggatc atcgtttcat
    20521 tggcaggcaa gcgattgatt tcgtgtgcat ctacaaatgt gccgtctgga actttgatat
    20581 atcctaaacg ttgaccgttg acgatggcat tttccatgct tcgtccgaaa acagcgattt
    20641 tacgaccagt tgccacagcg gcatctgctg cttgctgcaa acggaagatg ttagaagcaa
    20701 agctggcaaa aatgatacgt ccttcgattt ttgaaaagat cttcaaaata gaatgcccaa
    20761 tcgttttttc tgatttagta aatgttggaa tctcagcatt tgtactatca gataaaaggc
    20821 acaagactcc ttcttccccg atacgagcca ttttatgcaa gtttgctggt tcgccaacag
    20881 gagtgaaatc gaatttgaag tctccagtcg ctacgatatt tccagaaggt gttttcacca
    20941 cgatccctaa tgcatctggg atactatggg tcgttctgaa aaagctgaca gaagtctttc
    21001 taaaacggat gatcgtatct tcattgatct catggagctc tgcatctcga agcaagccat
    21061 gctcatctaa tttgtttgtg atcaacgcta atgcgagagg accagcatag ataggaatat
    21121 ttgcttgttt cagcaagtag ggtattccgc cgatgtggtc ttcgtgcccg tgagtaatca
    21181 ccaaggcttt tactttgtgt aagttttgta tgatgtagct gtagtcggga atgacatagt
    21241 caattccaag taaatcgtct tccgggaatt tgatcccggc atcaatcaaa atgatttcat
    21301 cttggaattg taccccatac gtatttttcc cgatttcacc taatccacca atagcaaaga
    21361 cgcctgtctc gttatttttg atggaaagtt tcatattaaa actccactaa tgagaaatca
    21421 gcattttctt tttcgtactc tagatgactt ccttctagaa gttgaacgaa ttcaatattg
    21481 tatggggtat tttcttccac acgttggcga gcgtctacat cgctttttgc ctcaatgtaa
    21541 agagattttg tttcttcccg tttagggttg cgtacttttg tttcttgata ataaacttta
    21601 tagatcattg ttttgtctcc tttatctgcg taaagcagtg atttagctag ttgatttttt
    21661 tagacaacac aaaacgaggg gacaagccac ctggtttttc atatgctttg actagaatac
    21721 tggattcgag tcaaagagac aagcctcgtt tttgcttatc tgttcggata ttttttcaac
    21781 cgtaccttct gtataaaaaa tatacaacaa ttaattgtta ttctagcata aaacagcaaa
    21841 taagtataga aatcaactag attgaaagaa aaaatcaatg tttatcatcc cgctataatc
    21901 ataatgactt ttttatgaga gaagtgttct attaataggc tcgttgtgct atcattttta
    21961 gtaatgaaaa tgtaacaaaa gattaaaagg agatgagaaa gtgaaactaa tgtggcatta
    22021 taccatgcgt tataagaaat tcttgttttt caatttcata tgcgtatttg gttttatttt
    22081 gattgaatta ggacttccta cgattttagc acgtatgatc gatgtaggaa tccgcaatga
    22141 tgattttgat tatgtgaagc aacaagggat atttatgatc gttattactg taatcggtat
    22201 tattttgaat atcttgttag gttattttgg ttcgaggatt acgactaata tcgtagcaga
    22261 tattcgagat gatttgttcg aaaagatcca aagttattcg catcaggaat acgaaaatct
    22321 aggcgtatct tcattgatca ctcgtacaac aaacgatgct tatcagatca tgttgttcct
    22381 gcaaaatatt ttacgtatcg gttttatggc accaatgatg ttcattgtca gtttgtacat
    22441 ggtcatgcgt acaagtgtga cactcagttt gtatgtagta ggtgcattac ctttactatt
    22501 attggcagtc gtagcgattg ctaaggtgtc tgaaccgtta tctaaaaagc aacaaaaaaa
    22561 cttagataaa atcaacagta tcttgcggga aaatctttca ggattgcgtg tgattcgtgc
    22621 attcgtcaat gaaaagtttg aagaaaaacg ttttgctgta gtcaatgaga attatgcttc
    22681 tagttcaaaa agcttattcc gtttgatggc tgttgcacaa ccgggttttt atttcttatt
    22741 taatatcgta atggtattga tcatctggag cggggcagtg caaatcgatg caggcgagct
    22801 tcgtgtagga gatttgattg cgtttatcga gtatatattc catgccttgt tctcattcat
    22861 gttatttgct agtgtgttca tgatgtatcc aagagcagct gtttcagctg gacgtatcca
    22921 agaagcccta gatgccaaac cttcgattgt agaagatcca aatggtatat ctgatacaaa
    22981 aactaaaggg tatatcgaat ttcaaaatgt aacatttgct tatccaggcc attcgcaaga
    23041 gccagtgata cgaaatgtca gttttacagc ctctccagga gagaccgttg cttttattgg
    23101 aagtaccggt agcggaaaat ctacgttgat ccaattgatc cctcgctttt atgatgtgag
    23161 tgaaggaaag atcttgatcg atggagtaga cgtgagagat tatcggctaa gcaagttgcg
    23221 agataagatt gggtttattc cgcaaaaagc gctgctattt actggaacga ttgcagataa
    23281 tttgcgatac gggaaagaag atgctacgca agaagaaatg gaacgtgcag ctgagattgc
    23341 tcaagcttca gaattcattt ctcgaaaacc agatggattt aatgaacatt tatcagaagg
    23401 tggttccaat ttttcaggtg gacaaaaaca acgattggca atagccagag ccatcatacg
    23461 ccgtccggaa atctatattt ttgatgacag cttttctgca ttagattatc aaacggatgc
    23521 gaagttacgt gctcgtctga aaaaagaaac gacagaatct actgtattga tcgtagcaca
    23581 acgtgtggga acgatcatgc atgcagataa gatcatcgta ttaaatgaag gagaagtcgt
    23641 cggtatgggg acgcatcggg agttgttgga aaaatgcccg atttattatg atattgccgc
    23701 ttctcaatta tcaaaggagg aattagcatg aaaaaaacct tttcttcctt aaaacgttta
    23761 gctcgatata tcagaccgta tcgtgagact tttcttttag tgatcctatt tacagttctg
    23821 acagtggctt tcaacgtggc tttgccatat gttacaggac ttcctactac agaaatcagc
    23881 cgtaatattg ccaatggcga atcaatcaat ttttcttata tattcaaatg tctcatttgg
    23941 atcacagttg ttggaatagg gtattgtatt tcgcaattac tttctggtgt tttgatgaca
    24001 aatgtcgttc aaagtgccat gcatgatttg agaagagaca tagaagaaaa aatcaatcgt
    24061 cttccggttt catatttcga taaaaaccag caaggaaata ttttgtctcg tgtgacgaat
    24121 gatgtagatg cagtcagcgg tgctttacaa caagctttta ttggtatcgt aaatgcgata
    24181 ttgggaatta cgttggctgc agcaatgatg ttttatatcc agccgatgat ggcgctgatc
    24241 tctatgatca tgattccaac atcgatctgg atctcaaaac gagtgatcaa tgcttcgcag
    24301 aaatattttc aaagcatgca gaattctctt ggtgagctaa acggctacgt ccaagaaaat
    24361 atgacaggtt ttagtgtatt gaaagtatat ggacgcgaaa aagaaacatt ggaaggattc
    24421 aaaaaagtta atcacagctt gaaatacttt gggtttcgtg cagcattcat ttctggcttg
    24481 atgatgcctc tagttcaatt aactgcctat gctacttata ttggtatggc tgttctagga
    24541 agcttttatg ttattactgg ggcgatcgtt gtcggtcaac tacaagcatt catccaatat
    24601 atttggcaaa tcagtcagcc aatggggaac gtgacacaat tgtcttcagt tttgcaaagt
    24661 gcatcagcgg caacgaaacg tgtattcgag attttagatg agccagaaga aaaagaaaac
    24721 aaagtagatg cttcactgcc agaaacgatt ttaggagatg tgacattcga tcatgtcgat
    24781 tttgcatatg atccgaagaa accattaatc caagatttga attttgaagt gaaagctggt
    24841 caaaccgtag ctgtggtcgg tccgaccggt gccggcaaga cgacattgat caatctattg
    24901 atgcgttttt atgacgtaga caaaggagca ataaggattg atggaatcga caccaaatca
    24961 atgagtcgca gtgatgtgcg ttcgctattt ggtatggtct tgcaagatgc atggctttat
    25021 caaggaacga ttgcagataa tatccgcttc ggaaagcttg atgcgacaga ttatgaagta
    25081 gttgatgctg cgaaaacagc aaacgtagat cattttatcc gaacgatgcc agatggatat
    25141 gatatggcca tcaattctga gggagacaat gtgtctttag gacaaaaaca gttgctgacg
    25201 attgcccgag cagtggtctc cgatcctaaa atcttgattt tagatgaagc aacaagttca
    25261 gtagacaccc gtttagaagc gttgattcaa aaagcaatgg atcgcgtgat ggagggacgg
    25321 acaagtttcg tcatcgctca ccgtttatcg acaatccgtg atgcagatct gattctagtg
    25381 atggatcaag gtcagatcat tgaaaaagga acgcatgaga gtttattggc acaaggtgga
    25441 ttttatgaaa aactgtataa cagccaattt gctgaggaag cagaataagt catataagaa
    25501 taaaacagtt taaaacttat tttttgttct ctattcttga taatggtaaa gtaaagttat
    25561 caagaaaagt gaggacacgg agatggaaca gtttttaaat gaaaaaatta ctgaagccaa
    25621 aagagaagtt gatcgtttaa cgcacgtctg tcagacgaag ccttttgatt cagctgattt
    25681 ttttgaaaat gagtttcaaa tcgaacgctt ggcagcacaa atagaagtgt acgaattaat
    25741 tttaaaaaaa gtaatagaag aaaatataga tgtacaataa aaacaggtaa aacgtcagtt
    25801 accagatact gtaaatagac atctggcctg agcaggtttt acctgttttt tttcttatga
    25861 aataaatata tgctatgata cctttattca ataggattag tttctacaaa aaatgaaagg
    25921 agaaaaataa atatggaacg gtttatagca tcaaatgcca cagtgattgg tgatgtgaca
    25981 ttgtcagaag atgtgacgat ctggtatcaa gctgttttac gcggggacag taattggata
    26041 aaaataggac aaagaacgaa tatccaagat ggtacaatca tccatgtcga tcatgatgca
    26101 cctgtagata ttgcggagaa tgtgacggta ggacatcagt gcatgctaca tggctgtaca
    26161 atcgaaaaag gggcgctgat cgggatgggg accacgatat tgaatcatgc ggttattggt
    26221 gagaatagtt tgattggtgc tggatcatta gtaacagaag gaaaagttat tcctcctaat
    26281 gtcttggctt ttggtcgccc agctaaagtg atccgtcctt tgacaaaaga agaaatacaa
    26341 aaaaacaaag aaaacatcca gcattatgtc gagataggtc ggatgcatgc gcaaggaaaa
    26401 ttcaaggagc aaagataggc agtagcatcc ataaaataaa aaaggatggt cagtttgagt
    26461 tgaccatcct tttttatttg gtttagataa gagagcttaa aaaagcttcc tttgaaacag
    26521 attaatttaa tgaagaactg ttttccatga cttcatcgat caatccatat gcttttgctt
    26581 gttcagctgt catatagttg tcacgatctg tatctttttc aatcacttca agcggttgtc
    26641 cagtgcgttc agctaaaatt ttgtttaagc gttcgcgtgt ttgcaaaata tggcgtgccg
    26701 caatttcgat ttcagttgct tgcccttgag cgccgccaag aggttgatgg atcatgatct
    26761 cagcatttgg taaagcaaat cgtttacctt ttgtaccagc tgtcagcaag aagcttccca
    26821 ttgaagcagc catacccata acaattgttt ggacatctgc tttgacaaag ttcattgtgt
    26881 cgtaaatagc catacctgct gtaactgaac caccaggaga attgatataa agatagatat
    26941 ctttttcaga atcttgagcg tctaagaaaa gcaattgggc gatgatcgaa ttagcaagat
    27001 catctgttac ttggccgctt aacatgatga tacggtcttt caataaacgt gagtagatat
    27061 cgtaagctct ttcacctcgt gaagattgtt caatgactgt aggaattaaa ttcataatag
    27121 agttcctcct tgatttcgtt tagaaatagt gtaccataat ttagtttcta atacctaaaa
    27181 tgtagtatac atttttggtc aaaaaaggtc aaataaaaaa cttaggcaaa ttagtcgcat
    27241 aaacccgctt tatggtatga taagcagaga aacaaaacaa ggaggaacaa cagattgata
    27301 gttggtatta ctggccttat cggagtgctg acaggtgctg tagttgtcta tatcggcatg
    27361 atactgcaaa tgcgtcactt ggaaaataaa gaacagcgta gacgtgaatt ggaattgaaa
    27421 gtcaaagaaa tcgagacgct gaatcaatta aataaaaaga tcaacgagat cttgcaaaaa
    27481 cggacaacgc tgatggaaga atatgtcagc tttgatgcat ttgatgattg ttatatcacg
    27541 attgacgatt ttgcgtatct ccaatcattt tcagcgcaaa atagttttta tctgccaaat
    27601 tatattttag acgagctatt taagaatatc tcccatcgaa aagtgatttt atctccagat
    27661 gaaacaatga agattggtgg atatgcttat aaaggcggac gcgtgatttt agagaatttt
    27721 acggatagtc tgactgaaat gatcaatgaa aaaaaagctc agttgaagac tatgacgcgc
    27781 cagccgattg gtttcttctc gaaagatgat gtttggagct gatattttga agcattgccg
    27841 ttgcacatga gagcgtggta atgctttctt tttttcttaa tgatacaata gaactgaacg
    27901 gagtgaagaa agatgttaca aatctatgcc catcgcggaa gcagcggcac ccacccagaa
    27961 aatacgcttc ctgcatttgc agaagctgta cgagtaggtg cagatggtat cgaactagat
    28021 gtccatctaa gcaaagatgg ttatttgatc gttatgcacg acgaagaagt ggaccggacg
    28081 actaacggta aagggctgat tcgagaaaaa acattggaag aactcaaaaa attgaatgca
    28141 gggggcaaat tcagcaaaga atttccagca gctaaagttc ctgaattgag agaagtgtta
    28201 ggactgctcc tccaaaaaaa ttaccgaggg atgctaatga ttgaaattaa aactgatcag
    28261 tatgaatatc ccggtatcga agaaaaatta gccgaaacat taaaaagtag agattggcct
    28321 tttcaacatg catattgcag ctttcatttg gattctttgg aaaaaatcgc attactggaa
    28381 ccggagatac agctggactt attgatgagt acttctgata aaaagatcca actagcagca
    28441 gaaacccctt atatagaagg agtacatcca aaaaatactt ggttgttcga acatccagtc
    28501 caggcagctg attttccaaa agcaattcgt gcatggacga ttaacgatga agaagaaatg
    28561 gccaaatgtc aagaactcaa aatagatgga ataataactg attttccgga gaaagctctt
    28621 cggtggaaac aatcaacgaa aagtaggtaa aatatggaaa aagtattagt gatcgtcggt
    28681 ccgacagcag tcggtaaaac agcgctaagt gtggaactcg cacaactatt tggaggagag
    28741 atcatcagtg gtgattcatt gcaaatctat aaaaatctgg atatcggtac agcaaaaatc
    28801 aaagaagaag agatgaataa tgttccgcat catttgattg atgtgatcga acctactcaa
    28861 cgttactcag ccgctgattt tcagcattca ggtcgacaat tgattaccga gattgcgaac
    28921 cgagggcacc tacctatcat agctggtggt acgggtctct atatccaatc ccttctatat
    28981 gattatcagt taggagcgac agaagaagcg aatcctgaga ttagaaaaaa gtatgaaacg
    29041 ttagctgaaa caattggttc ccaagcatta tggaatcagt tgaaagaaaa agacccggca
    29101 gctgctgaga aaatccactg gaacaatcaa cgcaaagtga taagagctct ggaagtttta
    29161 gagacgacag gacatagtat cacggcacct aaagaagagc caaagcaatt gtatgattac
    29221 ctcatgattg gattagatac gaagcgtagt attctttatg aacgaatcaa tcaaagagtc
    29281 gatttgatgc tagcagaagg tttagtagca gaagcgcgta aggtgtatga gcttggagaa
    29341 gtccaggcca gtcaaggtat tggatataaa gaatttttcc cttattttga agggaaagaa
    29401 acattggaag aagcaacaga acaagtcaaa ctacattcga gaagatacgc gaaacgtcag
    29461 ttgacgtggt ttcgtaaccg tttggatgca cagtggtttg atcttataag tgagccaaat
    29521 caaaaaagtc agattgagca aacaatcaaa aaatggctgg aggcggaata aaatggaaga
    29581 aaaagtgatc atcgtagggg tagaaaccga agcaaaccag cgttactttt cagagtcaat
    29641 ggaagagcta gttcagctga cgaataccgc atcaggagaa gtcgtattca caattacaca
    29701 aaagagacca caagtggatc ggcaaacgat tatcggtaaa gggaaattac aagaattagt
    29761 tcaacaagcg gatgcgcatg aagcggattt gatcatcttt aatcatgagc taactccgag
    29821 acagagccaa ctgattaccg atgctgttgg tcttccggta attgatcgcg tgcagcttat
    29881 cttagatatt tttgcgatgc gtgctcgttc gaaagaaggg aagctgcagg ttgaacttgc
    29941 tcagttagaa tacctgctgc ctcgtttagt agggcaaggg aaaacattat ctcgtcttgg
    30001 cggtgggatc ggaacaagag ggccaggtga aacaaagtta gaaacagacc gccgacatat
    30061 acggaataaa atttttgctg ttaagaaaga attgaaagaa gtagaagctc atcgagagcg
    30121 caatcgccaa aaaagaaaaa atagcgagat ttttcagatt ggacttattg gctacacgaa
    30181 tgccgggaag tcaactatct tgaatctatt gacacaagcg gatacttatt cgaaagatca
    30241 attgtttgca acgttagatc cattgactaa gagatggcgt tttgctgaag gatttgagat
    30301 tactgtaacg gataccgtcg ggttcatcca agacctgcct acgcaactga tcgatgcttt
    30361 ccattctaca ttagaagaaa gtcaaaatat ggatcttttg ctacatgtag tcgatgctag
    30421 ttctccagat cgtattttac aggagcagac tgtcttaaaa ttgatggatg aactgaatat
    30481 gaaagaaatg ccgatactga ctgtttacaa taaggcagac cagatcgacc cagcgatgtt
    30541 cacgcctacg ctattcccaa atgtattgat ttctactcag acgaaagaag gtaagatggc
    30601 gttgatcgaa gcaatcaaac gtcagctgat ggaactaatg accccttata cactttctgt
    30661 gccaagcaac gaaggacaga aattaagcga gttgagaaga caaacaatgg ttttggacga
    30721 agcgtacatg gaagaaacaa acgaatatga agttaaaggg tttgcaaaaa ccaattcaaa
    30781 atggttaaga aaaaaccaat aaaattttat ttttttaa
//
LOCUS       DACESX010000017        30742 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.017, whole genome shotgun sequence.
ACCESSION   DACESX010000017 DACESX010000000
VERSION     DACESX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.017"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(185..1768)
                     /locus_tag="IXF88_000677"
     CDS             complement(185..1768)
                     /locus_tag="IXF88_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1507426.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2346..2489)
                     /locus_tag="IXF88_000678"
     CDS             complement(2346..2489)
                     /locus_tag="IXF88_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1507427.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2712..3191)
                     /locus_tag="IXF88_000679"
     CDS             complement(2712..3191)
                     /locus_tag="IXF88_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1507428.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3264..3548)
                     /locus_tag="IXF88_000680"
     CDS             complement(3264..3548)
                     /locus_tag="IXF88_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507429.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3626..3820)
                     /gene="ssrS"
                     /locus_tag="IXF88_000681"
     ncRNA           complement(3626..3820)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IXF88_000681"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3892..5178)
                     /locus_tag="IXF88_000682"
     CDS             complement(3892..5178)
                     /locus_tag="IXF88_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1507430.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5316..5987
                     /locus_tag="IXF88_000683"
     CDS             5316..5987
                     /locus_tag="IXF88_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1507431.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5953..6441
                     /locus_tag="IXF88_000684"
     CDS             5953..6441
                     /locus_tag="IXF88_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1507432.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6454..7401
                     /gene="prmA"
                     /locus_tag="IXF88_000685"
     CDS             6454..7401
                     /gene="prmA"
                     /locus_tag="IXF88_000685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1507433.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7402..8166
                     /locus_tag="IXF88_000686"
     CDS             7402..8166
                     /locus_tag="IXF88_000686"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1507434.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRISAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8399..10612
                     /locus_tag="IXF88_000687"
     CDS             8399..10612
                     /locus_tag="IXF88_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1507435.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10623..11069
                     /locus_tag="IXF88_000688"
     CDS             10623..11069
                     /locus_tag="IXF88_000688"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1507436.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11240..12499
                     /locus_tag="IXF88_000689"
     CDS             11240..12499
                     /locus_tag="IXF88_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1507437.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12588..13535)
                     /locus_tag="IXF88_000690"
     CDS             complement(12588..13535)
                     /locus_tag="IXF88_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1507438.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13929..15221
                     /locus_tag="IXF88_000691"
     CDS             13929..15221
                     /locus_tag="IXF88_000691"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1507439.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15236..17005
                     /gene="aspS"
                     /locus_tag="IXF88_000692"
     CDS             15236..17005
                     /gene="aspS"
                     /locus_tag="IXF88_000692"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1507440.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            17114..17989
                     /locus_tag="IXF88_000693"
     CDS             17114..17989
                     /locus_tag="IXF88_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1507441.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18255..20138
                     /locus_tag="IXF88_000694"
     CDS             18255..20138
                     /locus_tag="IXF88_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1507442.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20213..20497)
                     /locus_tag="IXF88_000695"
     CDS             complement(20213..20497)
                     /locus_tag="IXF88_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1507443.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20487..21221)
                     /locus_tag="IXF88_000696"
     CDS             complement(20487..21221)
                     /locus_tag="IXF88_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1507444.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21350..21973
                     /locus_tag="IXF88_000697"
     CDS             21350..21973
                     /locus_tag="IXF88_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1507445.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            22165..23286
                     /locus_tag="IXF88_000698"
     CDS             22165..23286
                     /locus_tag="IXF88_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1507446.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23293..26421
                     /locus_tag="IXF88_000699"
     CDS             23293..26421
                     /locus_tag="IXF88_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1507447.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26440..29634
                     /locus_tag="IXF88_000700"
     CDS             26440..29634
                     /locus_tag="IXF88_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1507448.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29780..30487)
                     /locus_tag="IXF88_000701"
     CDS             complement(29780..30487)
                     /locus_tag="IXF88_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1507449.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10024 a         5593 c         6527 g         8598 t
ORIGIN      
        1 agaaaaaaca tttttagatc agcctcgtgg gctgtcgact ttattcttcg ctttatgttt
       61 agctagaaaa ccgatccgaa acattcattg atcgaataat aaaaacttga ttttccacct
      121 gtactttgcc tgaatcgtga aagcacaggt atgaaaatca agttttttgt tgcgaaaaaa
      181 cgtcctagtc aatcagttgg ccgagcggct ggcggctgat ctgctcctga tcaataacga
      241 gtgcttcttg gaagtgttgc tgcaaaaagt agcggtcatg gctgactgct aggagcgttc
      301 cttgatagtc ttgcagcatt tcttcaattt cttctcgagc atagctgtcg aggtgattgg
      361 ttggttcatc caaaatcagg acgttgattt tttctaaaaa gagtttcagc aacgccaaac
      421 ggattttttc accaccggat aaatctttga tccgtttacc cacatcgctt tgataaaagc
      481 cacttttggc taattgccgt cgagcgactt cttcgtttcc agtcaatgtg atggcgtaag
      541 ccagcaaacg gctatcggga tcggcaaact gcagctgctg cggcaaataa ccgatttgca
      601 agctactacc taatttgatg gtgccaccgt aaggcttttc ctcaccgagt aataatttga
      661 gtaaggttga ttttcctgca ccattgtcgc cgatgatggc taaatgatct ccacgataga
      721 ctgcaaaact gctgtctttg aatagtactt gatcagcgta ctgctggtgc aagttttgaa
      781 tgatgaaaac ttctttgcca gattttccac cattagacaa gctttgcagc cgatttttcg
      841 ggggaggtgg cggtttgacg agtgtcagtt tcgccaggcg gcgttccagt tctttggctt
      901 ttttgaagaa ggactcattg tctccttcat gtgcccactg acgaaaacga cgaatcatga
      961 ctttcaaccg ctggacttct ttttgctgca gttcgtaatt ttttgtcagc tcagccaagc
     1021 gagcttcttt caagactcga aaacggctgt agtttccggg atactcaatc aatgcacctt
     1081 cttccagttc ccatgttttt gtggtgactt gatcgagaaa ttgccgatca tgagaaacga
     1141 ccagaaccga tttttttgta ttctgtaaat agttttccaa ccagcgaata ctgttaagat
     1201 ctaaatgatt ggtcggttca tcgagtaaaa gtaaatcggc atcggctgct aaaattttgg
     1261 ctaaagcgac tttaactcgt tcgcctccgc taagttctgt taaccgtgct tcctgacggt
     1321 gtggaatccc taagccttgc aagatcgctg caatccggtc ttctaatacg taaccgccta
     1381 gttcttcaaa gcgatgctgc agctcaccgt aacgggccag acacttctct aagtttgtat
     1441 ctggttctgt catcaatgct tcgtagcgac tcatttgctg tttgaggtcc tgtatctcag
     1501 cggcagaatg gagcaagtag tggttgacgg tcgcttcgga aactgtaagt tcttgttcga
     1561 ccgtcccgat ctttaagcct tttttgcgac tgataacacc gctgtcgaca ccttctctgc
     1621 cggtcaagat ttgtaaaaag gttgatttgc cagttccgtt gataccgact aaggcaattt
     1681 tttcaccagc ttcaatttga caggataaat cctcaaataa tggattcccg ccgattgttt
     1741 ttttaataga aattgcttgt atgatcatga tctctctcct tgagaccact caaaaaggcc
     1801 atggttatcc atggcctgtg ttatttaaac tcagattatc atgagaaaaa taccttgaga
     1861 tggccattga ttttacgtga attcaactgt tttggacaaa cttgtcccgt caacagcaac
     1921 tgtaaaacca attgcatggc taaatgcgat atggtatcgt tggtaatttt cattacttgt
     1981 tattttagtc atgcgcatca tagtattttc cctccttcgt gtagttgtct tttccagtgt
     2041 acctgaaagt gtcagaaagt tcaactagcg ctgacattta cttaatattt atcgatgtgt
     2101 aattctgtca gagagctttt tttatctgac tgagggtaat gtctcgtgga ccacatcatt
     2161 gtattctgag ctaaaccagc gttcaggcga aatcatatgg tgtttgataa aaaactttgt
     2221 ctacatgact tccttttgcc atttgcttaa taccgttgca ttaaacagct cttctctttg
     2281 gaaatctcac caacatcgcg ttcaggagta tcgttgtggt tgcaatggca agcggtagcc
     2341 tttcatcaat tcttgaatga tttaccgaat gtagcacttg ccttctcgcg gtacccgaac
     2401 ccgaaataaa attttagttt ggcgataaaa tcggtatccg ccatcgatac ttcatatttt
     2461 ttcgtatcag attcatcgga aacttccaca ttgacgaacc aataagcttt ggcatgctta
     2521 ggtcgtttta aaccacacca gggcgactaa tcgccctggt gttcactttt ttttatttta
     2581 cttgaactca ttgactgttt tccctttatt ttaaacatta taaagaccga tgagtgcttg
     2641 ccgcgtcttt tgtttttatc acggtttttt gtgcaaaaaa acctgccgat agttggcagg
     2701 tcgtctacgt gttaagcttt ggtgtggtct ttagctggca attggttttc taaatcagtt
     2761 cggacaacaa gcacatcgca agctgcgttt ctaataacat attcagaaac agaaccaata
     2821 aataaacgtt ctacagcatt caatcctgtg gctcctaaca taatcaaatc tacttgatta
     2881 tcttctggga tttctctggc aataatttgt tttggtgagc catattctac gacagaggtt
     2941 acattattca agccagcttt cttcgcattg ctttcataat ctgccaatgt ttgctttgcc
     3001 atttcagtag cttgttctgc aagcattcca tcaaaggatg aaacggtttg gaaagaacgt
     3061 gtgtcaatga catgtgctaa tagaagttct ccgttatttc ggatagctac attgaccgcc
     3121 tttttaaaag ctaattcagc ttcatcagaa ccatctacgg caaccatgat ttttttgtat
     3181 tgttgtaaca taagcattca ctcctcaagt caatcgtatt gtactctttt attgtagaac
     3241 actttaggga aaaagagaat tatttactgc tttggttcag caattttttt tgcaaatcgg
     3301 atactgaagg tggcagaaac aataattacg atcagtaaaa agaccaaggc aataaagcgt
     3361 tgattgaaaa aagctgttaa aatacccatt acgcctaaaa cagcatatgc agctccgtaa
     3421 aattgtagga atccttgatt tttatcattt ttgttgatca atacaaaaaa gatatcctgt
     3481 ttattaaata aataatagct aacgaataat aatactaaag tggcgataat cattaaaatc
     3541 ctaatcatga acaaatcctc caaaataagc aacaaataga aaaagcaatt tgccaagagc
     3601 aaattgcttt attctttaaa agatggtaat ctccgttaat gccgttgttt gtcctgtagt
     3661 attgatcccg cgaattcaag caggtgggtc ccatggtcgt agcttcgaac taccaaatga
     3721 taaggcatgt taccagcagt gacacaagac gggtcccaag atatcaataa aaatgttcgg
     3781 tcaacacaca aagaggacaa ttcgaacatc acagatttcc cacctctata gtagcatatg
     3841 aatttaaaaa gagcaatgag agagctcgca gaaacagaac cgttttcttg tctagttttt
     3901 tggaggatgc tttttccact cttttatacg gtaatactgt tgacccagag cctgttcata
     3961 tttccctgta tcttttggct gataatactg agcattcttc agtttatctg gtaaatattg
     4021 ctgattgacc caagcctgat caaaatgatg aggatactga taacccacgc cacggttgag
     4081 tgatttcgca cctttgtaat ggctatctct caaatgatca ggaacatctc cagctttgcc
     4141 ctctcgaata tcagcaagtg ctgcatctaa tgccatgtag gcagaattgg attttggcga
     4201 caagcaaaga tcgacgacga cgtctgctag tgggatacgt gcctcaggca atcccaattt
     4261 ttcagcagct aatacagcgt tcactgtacg agccgcagca gcaggattac ctaaacctat
     4321 atcttcataa ccgataacca tcagacgccg gcaaatactt gccagatcac ctgcctcaac
     4381 taaacgggcg agataatgaa gtgcagcatc cacatcactt ccacgaatcg atttttgaaa
     4441 agccgaaata acgtcataat gagcatctcc atttttgtca tgggtaagag cttttctttg
     4501 gacacattct tcaatgatcg acagtgtaag atgaatcctt ccttctttat ctgaaggagt
     4561 cgatagtgta gccagttcca gcccattcaa tgcactccgc aaatcgccgt tcgttgcacg
     4621 cgaaaggtga agaagagcat cttcgtctaa ctggattgct tgttgtccaa ggccgcgttc
     4681 tttatccctt aaagcatgtt cgactgccaa ctggatgtct tgttctgtta atggtttgac
     4741 ttcaaagatc tgtgtacggc tgcggatcgc tggattgatg gtaatataag gattctcagt
     4801 agtcgctccg atcaagatga ttcggccact ttctaaatga ggaagcagaa aatcctgttt
     4861 tgttttatct aatcggtgta cttcatccaa taagaggatc actgtaccac tcatttttgc
     4921 ttcttccgct acaacttgta gatccttttt tgaatctgta gcggcgttta gcatgcgaaa
     4981 tgcataatta gtagagccgg cgatcgcact tgcgatactc gtttttccag taccaggagg
     5041 tccatataga atcatggaag agagcatgcg agcatcgacc atgcggcgta tgatttttcc
     5101 ttccccgact agatgctgct gaccaacgac ttcatcgatc gttcgaggtc tcatgcgata
     5161 agccaatggc tgttgcatag gattttcctc cttcttgttt ttacgtccct taattatatc
     5221 atatttagca agatgcctag aaagaacagc cttctgttgt tttgttttca tagcttatcc
     5281 aactaatatt gtgtatgata aagtaggtga gaaaaatgac ggataaacaa aagatagatg
     5341 aatttttttg gaataaaacg acgccagaag tggcacggga tttattgggg atgtatttgg
     5401 agcatgacac accagaagga agattagctg gatatattgt agacgcggaa gcatacctag
     5461 gtccagaaga cgaggccgcc catagctatg gtttaagaag aacaccgcgt gtgcgggcaa
     5521 tgtatgaaaa accaggtacg atctaccttt acacgatgca tactcatcgg attttgaaca
     5581 tcattactca gccagaaggg attcctcaag gagtaatgat acgtgcaatc gaacccgccg
     5641 caatgattga tcaaatgagt aaaaatcgtg gcggaaaaac tggtccagat atcagcaacg
     5701 gtccgggaaa attagtggaa gcattagcaa ttcctcaaga actttatggt cagtctatag
     5761 cagatagttc gttacgctta gtttttgaga aaaaaaagac accaaaaaag atcaatgctt
     5821 tgcctagaat cggtatccca aacaaaggtg tatggacaga aaagccgttg cgttttgttg
     5881 tgtcgggaaa tccttatatc tctttacaaa gaaaaaacca aatcgagaaa gattggggtt
     5941 ggaggaaaga aaatgaaaaa gaaggatcta ttaacatatt tagatgaaat aattgaaaaa
     6001 caagtgacag actatgatgt agctttagat tgggatacaa aaaatcatac gatcgagatc
     6061 gttgttcgct tgtttgcaga aaataaagca caatttgaga ttgatgatgc agaagggatc
     6121 gtttcgcaag aaccaattat cgaatttgaa gacggcgtac ttctttttaa tcctcagaaa
     6181 tctgtttttg atgaacgcaa ttatctagca gtaatccctt atgaaggaaa aaaagggttg
     6241 gcaaaatcag tagctgattc tttagtggaa tatctaaatg aagtattagc tcaagggcaa
     6301 agcgatttgc ttgatttttt ggatgaagac gatgacgaag ctgtattcga gcttcactgg
     6361 gataatcagc aactagcaga attagtagaa gtaaaacagc aaaatgaagc agatacttat
     6421 ttaccgtacc caagttatta aggagtgaca gatatgaaat ggaatgaagt aaaagtagaa
     6481 actgaaagtg aagcagtcga agcagttgct aatattttga tggaggcagg ggcgagtggt
     6541 gtcgcgattg aagattcttt agatgttgaa cattttcagt cggatcaata tggtgagatt
     6601 ttagataaaa atgattttac atcgctgaaa gaaggcgcac tagtcatggc ttattttccg
     6661 gaaacgactt tcttaccgga aatcttgcct tttatcaaag atcgtatcac acaattacct
     6721 gagtttggtc tagctattgg aaaaaataaa gtaacagtta gtgaagtaga ggaaagcaac
     6781 tgggcaacag cctggaagaa atactatcat ccggtgcgta ttacgcgaat gctgacggtt
     6841 gcgccaagct gggaaaacta ccagacagaa gatcctctag aaaaaattat cacactagat
     6901 cctggtatgg catttggcac aggcactcat cccacgactt ccttgacatt gacagcacta
     6961 gaaacagtct tgcgtggtgg tgaaacattg ctagatgtgg gaactgggtc gggcgtgcta
     7021 agtattgcag caaaatatct tggtgcaaaa gatgtctatg cctttgattt agacgaagta
     7081 gccgttcgtt cggcgaaaga aaatatggat atgaatgaaa tagccaaaga tgtccatgta
     7141 tcagctaacg atcttttgac aggaatcgag attgaaagtg atgtaatcgt tgcgaacatt
     7201 ttagcagata tcatcttatt gatgatccca gatgcttggc gtttactgaa gcaaacaggc
     7261 acgctgatcg tttcaggaat cattgaagag aagaagcaaa tggttctgga tgccatgtta
     7321 gaacaagggt ttatcgtgga tcaaattttc cagcaaaaag actggtatgc aattattttg
     7381 aaaaaaccag aggtggaata gatgcagcgg tattttttaa aagaatcata tgaatcaaaa
     7441 gactattttg ttttagaagg ggaaaactat caccacattg tacgggttat gaggatggaa
     7501 cccgaggatg aagttttttt ggcattcaat gatcaaatag ccattgttgc agaaatcacg
     7561 gagattactg aagaggctgt ttatctgaaa gaaattaaaa aagaagaaaa tcaaaaggaa
     7621 ctgccaagtt cgattaccat agcgagtggc tatcctaaag gggaaaagct ggattggatc
     7681 gttcagaaat ccactgaatt aggagcgcaa gcttttcttg gtttcccggc atcagcctct
     7741 gtcgttaaat gggatgcaaa aaaacgtatg aaacgacaac aacggcttga aaagattgct
     7801 caagaggcag cggaacaatc ccatcggcag atcgtgccat cagtagagtt attggaaacg
     7861 aaacaagatt tatttgagcg gctagatgat tatgacgtgg tcttggttgc ttatgaggaa
     7921 tcagcaaagc aaggcgaaaa atcaaatgca gccaaaattc ttagtgaaac aaaaccagat
     7981 agtcgaatct cagctgtctt tggaccagaa ggtggattat cccgcgatga agtcgattat
     8041 tttgtagaac acaaagggat actttgtgga ttaggaccaa gaatccttcg tacagaaaca
     8101 gcacctcttt atttattaag tgctgctagc tatcaatggg aattaatgaa tccttctttc
     8161 agctaacgtt cttcattaat aaaagagtat cgacagaaag aattgaaaaa atagtcaaca
     8221 atttgtataa tagaaacaat tatttataca atggatacga aaaatcgttt gaaaaagctt
     8281 ttaaaaagcg aacatttccg taaaaaaacg tataactgca cggattttgg gacaattatc
     8341 aaacagatat ttgtcccgat tcccgtttta ccgaaaaaag aaagggggat cgattcctgt
     8401 gacaaaagaa gaaattttaa cgggacccgg tgtcataaag ctcgttagtc aatacatggg
     8461 tcccgaacat gtagcatttg ttgagaaagc atgcgaatac gcgactgctg cacatgacgg
     8521 acagtttaga aaatcaggcg aaccctacat tattcatcct atccaagtcg caggtatatt
     8581 agcagattta aaaatggatc cccatacagt ggctacaggc ttcttacatg atgttgttga
     8641 agatacagaa atcactttag aagatctgag agaagaattt ggcgatgacg ttgctatgtt
     8701 agtggacggc gtaaccaaat tagggaaaat aaaatataaa tcccacgaag agcagctagc
     8761 agaaaaccac cgaaagatgc tgctagcaat ggctcaagat ttacgagtta tcatggtcaa
     8821 attagctgat agactacata acatgcgtac gttgaagcac ctgcgagaag ataagcaaag
     8881 aaggatcgct caggaaactt tagaaatcta tgcgcctctt gcacatcgtc tagggatcag
     8941 ccggatcaaa tgggaattag aagatacagc acttcgttat ctaaatccaa aacagtatta
     9001 ccgtatcgtc cacttgatgc agacgaagag agaagaacgt gaaaaatacg taagtggtac
     9061 tgttgaagat attcgaatag cgacagaaga gctggggatt tttgcagaaa tctatggacg
     9121 gccaaaacac atttattcaa tctatcgtaa aatgaaagat cagaaaaagc agttcaacga
     9181 aatttatgac ttgctagcta ttcgagtgat cgtagattca atcaaggatt gttatgctgt
     9241 attaggagca atccacacaa aatggaaacc aatgcccggc agatttaagg attatattgc
     9301 catgccaaaa gctaatatgt accaatcttt gcatactacc gtgatcggtc cggcaggaaa
     9361 tccggtagaa attcaaatcc gaacacagga aatgcatgaa atcgctgaat tcggggttgc
     9421 tgcacactgg gcctataaag aaggaaaaaa tgaaaaagta gaaccagatg gtatgacgaa
     9481 acaattaagc tggttccatg agatactcga acttcaagac gaaagctatg atgcttctga
     9541 atttatggaa ggcgtaaaag gagatatctt tagtgataaa gtctacgtct tcacaccaaa
     9601 aggagacgtt actgagttac caaaaggatc cggaccattg gactttgcat acagtatcca
     9661 tacagatatc ggtaacaaaa ccactggtgc aaaagtaaat ggcaaaatgg tgcagcttga
     9721 ttacaaattg aaaaacggag atatcattga gatcatgact tctccaaatt catttggccc
     9781 aagtcgcgac tggttgaaat tagttgctac tagcaaagca agaaataaga tcaaacgttt
     9841 cttcaaagcc caagatcgag aagaaaatgt gatcaaaggc cacgaatccg tggtcaaatg
     9901 tattacagat ctaggattta cgcctaaaga tattttgacg aagaacaaac tgcaagaagc
     9961 actcgatcgt tttaattatc aaacagaaga tgatctctat gcagctgtag ggtatggaga
    10021 agttagcccc ttgacgatgg ccaatcgtct gactgaaaaa gaacgtaaag aacaaaaaat
    10081 cgagcagcaa aagcaagaag cagaagaaat catgaatcag ccgaaaaaag aacctgacaa
    10141 aatgaaagta cgtcatgaag gtggcgttgt cattcaaggg gtagaaaact tactaattcg
    10201 tatcagtcgc tgctgtaatc cgattcctgg tgatgatatc gttggttata tcactaaagg
    10261 cagagggata tccattcatc gtcgagattg tccgaatgtt cagcctgaca aaccaaatgt
    10321 agcagaacgt ttgattgaag tcgaatggga agatacatcg aatacacgaa aagagtatga
    10381 tgcagatttg gaaatttacg gctataatcg ttcaggctta ttgaatgatg tacttcaaac
    10441 agtcaatgcg ctaacgaaaa atctcaacag cgttgaagca cggacgaata aagataaaat
    10501 ggcgacgatc catttgacgg ttggtatcca gaatttatcc cacctaaaga gtatcgtgga
    10561 taaaatcaaa gcagtacctg atgtctacag tgtacgccgg acgaatggat aggaggacag
    10621 atatgagagc agttgttcaa cgggtaagta aagctgctgt gtcagtcgat cagcaagaaa
    10681 ttggaaaaat tggctacggc ctacttgttt tattaggcat tcatgaaacc gatacgcaaa
    10741 aagatgtgga ctatctggta aaaaaaattg cacaaatgcg tatttttgaa gatgaacaag
    10801 gaaaaatgaa tattagtgta gaagatgtgg gaggacaaat tttaagtatc tctcaattca
    10861 cactgtttgc ggatacgaaa aaaggaaatc gaccaagctt tgtggaagcc gcacgaccag
    10921 aaacagctat tcctttatac gaatcattca atgaaggaat cagaagtcgc ggcatcacag
    10981 tcgctaccgg acaattcgga gcagatatgt cggtttctct cgtcaatgat ggacctgtaa
    11041 cgatcattat tgattctaaa aacaaataaa gtaatctggt tttcactagt aaaaactatg
    11101 aaacacgtcg cttaagttag actgataaac tctaacttaa gtggcttttt tgtctgcaaa
    11161 aagcgcttct tttgtcagca gtcggaaaaa aagttaagct taaattatac aagttatttt
    11221 ttataggagg ttgctgaaga tgaaagaagt accagtagta gatatcaaaa tcacagattt
    11281 aaaaaaattg tatcaaaaag gatacaacat gttggaggag ttacgccatg aagcggacgc
    11341 tcccgtcgtc aaagcaaaga ttttcaataa agaagctatc acgatctacg gatcatcagc
    11401 agcaaaagta ttttatgatc caaggaattt caaacgaaaa ggtgctatgc caaaattagt
    11461 attgaagaca ctttttggtc aaggtggagt acagactttg gatggagctg ctcatcacca
    11521 tcggaaaaat atttttatgg atctaatgac accggaaaga atggaagatt atcatcgtat
    11581 cttagataag aatctgacac aagcacttga agcacagcat ggtcaatttg aactatttga
    11641 tttaagtaag atggttttct ttacatctat ttgcgagtgg gcaggtatca atttatcagc
    11701 gatttctaaa gatgaggtcg aaaaattagc agaatatcaa atctctatga tcagcggaac
    11761 gtttacttct ccgattgatc acataaaagg tgtcgaaaat cgcaaaaaat cagaaaaatg
    11821 ggcgcaaggc ttgattgaag aagctcgaca aaatcctgtg gctggaaaag aaaatgtggc
    11881 attatatgct tttgccaatg ccacagattt agatggtcag cttttacctc tggaagtggc
    11941 agctgttgaa ttattgaaca tcatccgtcc aacggttgct ctaactgtgt gggcggcatt
    12001 gatgggacat gcacttttca gtagaccaga tttgtatcag caattaaaaa atgacttttc
    12061 gacgttacaa gatccgttta ttcaagaaat gagacgctat tatcctttct tcccaatgct
    12121 acctgcgatt tcgctgaaag aagtagaagt agatggttat cgtataccag aaggcagttg
    12181 ggttatttta gatttatatg gaacagacca tgatgaacga acagttgaag cgccagattc
    12241 ctttatgatc aaacggtatg tagggaaagc aaaagatatt tcctataaag aagaatatga
    12301 gatgattgca caaggcgggg gaaattttcg acaaatgcat cgctgtgcag gagaatggat
    12361 cactcttcat agtctacgtg tgttttccga tcagctagta aataagtttg agttctctgt
    12421 acctgaacaa gattggacga ttccattcaa tcagtttccg acttatccga atagccgagc
    12481 gctactttac aagaattaga ttagcataaa cttatcgaaa aaaaggaaga tcaaacagtg
    12541 ctgatcttcc ttttttcata ttactataaa ttgataaggt caattgatca aaatccacga
    12601 aatgcttcat caatggtttg ataatcttca ttggataaat gcaaatctaa agcttggaca
    12661 ttgtcagata cttgctctgg acgcttagct ccaggtataa ctacagacac tcgtggattc
    12721 ttcatgtacc aagctaaaac aagttgcgca ggagtggctt ggtatctttt agcaatgggg
    12781 cgaagtacat cgacagcggt taaaatttct ctgaaacgtt ctccttggaa atctggattt
    12841 ctttttcgag gatctccttc accaaattgt ttttcctcac caagttcata ttttcctgtc
    12901 agcaaaccgg aagcaagagg aaaataggga acaaaactga ttttgttttt ttcaagataa
    12961 gggaataatt ctttttcagc ctgccgatgg atcaaactgt atttatcttc tactacatcg
    13021 acatatccat ctgcattcgc ttctttcaat tgttctaacg tgaagtttga cacaccgact
    13081 gctcggattt ttcctgcttc ttttaattcg tgcaatgtag caactgattc attttttggt
    13141 gtgctttcat ccggaaagtg gatataaaaa atgtcgatgt aatcagtttg cagacgtttt
    13201 agtgcatctt ccaccgattg tttgagaaac tcaggagaat tatcaaatgt tcgcccttta
    13261 tttggtacat gagcagcttt tgtagcgatg acgacacgtg agcgatcata ttctttttcc
    13321 ttcagtacct cgccaatcag ttcttcggaa cggccgtttc cgtaagcata agcggtatcg
    13381 atcaaattga tcccgctatt caaagctgta cgaactactt gttttcctgt ctcatcttcc
    13441 aaaccgtcaa aaagattatg tcctccaacg gcatttgctc ctaatccaag ctgttctgcg
    13501 tagacttgtg aatgtccgat tcttatttct gccatagctg attcctcctt tttgtctagt
    13561 ctaaaacaaa ataccaaaaa gctgaactaa aacgtttttg tgaggggaaa tagtcagata
    13621 tctcttgact ttgagactgg tttttagtag gttataagta atatcgaatg tcaatgaaag
    13681 agcgagtaag tttttgaaac tttacaagag aggatcatcg aggctgaaaa tgatctaaag
    13741 tttcattagc ctaaagacac tctggagatg gacgagtgaa aagccgtccc gcgtacgagc
    13801 gttattcgta agaggaaaga gaaatctttc aaaattaggt ggtaccgtgt atgaatttca
    13861 ttcacccttg ttcatgatga acaagggtga tttttttatt tacatagcta agaaagggag
    13921 agaaaactat gagtttccaa agaccaaaag gaacaaatga tattttgcca ggcgaatcag
    13981 aaaaatggca gtttgtcgaa gaaacagcac gtttattatt cagagattat cagtataatg
    14041 aaatgcgtac accgattttt gaacactatg aagtgatatc ccgaagtgta ggggatacaa
    14101 cggatatcgt ttcaaaagag atgtatgatt tttatgacaa aggggaacgc catgtgacgc
    14161 ttcgaccaga aggtacagca ccaatcgttc gttcttttgt tgagcataaa ttgttcggcc
    14221 cagagtatgc gaaaccgttc aaaacttttt atatgggacc aatgtttcgc tacgaacgcc
    14281 ctcaaaaagg acggttacgt caattccatc aaatcggagt agaagcattt ggatcagaaa
    14341 accctgcaac tgatgtggag agcatggtga tggcacttga tttcttcaaa cagttgggga
    14401 tcacgcaaat ccggctagtc atcaattcat taggtgataa agaaacgcgt caagcctatc
    14461 gtcaagcatt gatcgactat cttttacctt ttgaatcaga gctgagtgaa gactctaaac
    14521 gccgccttca tgaaaatcca ttacgcgtat tggacagtaa agacaaacga gatcaaaaat
    14581 ttgtggcaga agctccatct atcttagatt atctaagcga gcctgctcaa aaacattttg
    14641 ataccgttgt ttccatgctg gatgcattgg ctgttcctta tgaaatcgac agtaatatgg
    14701 ttcgcggact agattattac acacatacga tttttgaaat catgagtgac gcaccaaaaa
    14761 tgggggcaca agcaacgatt tgcgcaggcg gacgttatga caatctagtg gaagaactag
    14821 gtggtccttc aacacctggc ttcggatttg caatgggtat cgagcgatta ctcttgacta
    14881 tggaggcaga ggaagtagtt atccctgcat ttaatgagtt ggatgcttac gtggtcgcac
    14941 tcggagacga aacaaatatc gaagcattga aagtggtcca agcgattcga aactttggtt
    15001 tttcagctga ccgcgacttc atgaaccgta aagcaaaagc gcaatttaag acagcagata
    15061 aagcgaatgc caaattagta ttggtgatcg gtgaagatga gcttgctagt caaacgatta
    15121 atgtcaaagc aatggcaagc cgaaaagaaa aaagttttcc attagaagag atatatgaac
    15181 attttgatga agtctatgat gaaatgactt tattcgagta ggaggaaaaa aataaatggc
    15241 acaaagaacg atctattgcg gattagtttc cgaacaacaa ttagaacaaa cagttacatt
    15301 aaaaggatgg gtacaaaaaa gacgagacct tgggggagta atctttatcg acctacgtga
    15361 tcgtgaagga atcgttcaaa tcgtctttaa tcctgaaatc aacaaagaag catgggaaat
    15421 cgcggataaa tgtcgcagtg aatacgtgat cgaagtgact ggggaagtta gaagacgcgg
    15481 agaaaatgca gtaaatccta aaatggccac tggtgaattc gaagtgattg caagtgaaat
    15541 cgtgattttg aatcaagcaa aaaccccgcc atttcaaatt gaagatgatc aagcaatcag
    15601 tgacgaaata cgtttgaaat atcgttattt ggatctgcgt cgtccaaaaa tgactaaaaa
    15661 catcaaatta agaaatgcga caacaaaagc gattcgtcat tatttagatg ataatgactt
    15721 tctcgatatc gaaacacctt acctagggaa atctacacca gaaggcgcaa gagactacct
    15781 agttccttca cgtgtccatg ctggacattt ctatgcgttg ccgcaatctc ctcaattgtt
    15841 taaacaattg ctgatgaatg ctggattcga ccgttattat caaatcgttc gttgtttccg
    15901 cgatgaagat ttacgtggag accgtcagcc tgaatttacg caagtagata ttgaaacaac
    15961 gttcttaaca gcagaagaaa tccaaactta cacagaggga ttgattgcga aggtaatgaa
    16021 agaagttcgc ggtatcgaag tcacattgcc atttcctcgt atgacctatg atgaagcaat
    16081 ggctcgctac ggcagcgaca aaccggatac acgttttgat atggaattga tcgatctaag
    16141 cgatacagtc aaagaagtag aattcaaagt cttccaaatg gctcttgaaa acggcggtgt
    16201 cgtgaaagca ttgaatgcta aaggcgctgc agatcgctac tcacgaaaag acatggatca
    16261 gttaggtcaa tatgtaggac aattcggagc taaggggctt gcatggctga aagtagaaga
    16321 agacggattg aaaggtccaa ttgctaaatt catgggagaa gcaacagaag caatcattaa
    16381 agcaacagat gcaaaaccag gtgacttgct gatgtttggc gcagataaaa gtgaaatcgt
    16441 cgctgctgca ttaggtgcta tccggacacg tttaggaaaa gaattaggac taattgatga
    16501 atcgaaattc aatttcttat gggtaactga ttggccacaa tttgaatttt cagaagaaga
    16561 aggtcgatat gtctctgctc accatccatt cacgatgcca aaagaagaag atatcccgtt
    16621 attggctgaa gaccctgcaa aagtctatgc agaagcttat gatatcgtct tgaacggtta
    16681 tgaactaggc ggaggttcta tccgtattca tactcgtgag cttcaagaaa aagtactaga
    16741 aactctaggc tttacaaaag aacaaatgga agaacaattt ggtttcttgt taacagcttt
    16801 agattacgga ttccctccac atggcggtat tgcattagga ttggaccgtt ttgcaatgtt
    16861 attagccgga gaagataata ttcgtgaagt cattgccttt cctaaaaacg gaaaagcagc
    16921 agatgtgatg agtgatgcac caagtatggt ttcgccttta caattgttcg aattgaatat
    16981 tgatgtaacg aatatagaag aatagccgat aaaaaggtaa aaaggatttg tcgaatacgc
    17041 ttcttttgac aaccttgatt taattgaaaa acactttata atgaagccat aacatataaa
    17101 gcggggcttt tccatgaaaa aaacattcga aaattgggct tatcatgatt acacgaccaa
    17161 gctttcggta tctatcgttt atgcgttgat cgcatcagtc gcattgaatt tcttttatca
    17221 gccaggacat atttattcaa gtggtattac tggtctagct cagattttga cgactttgtc
    17281 aaaatcactg ataggattcg aagtaccggt atcaacaact ttgtatttac tgaatatacc
    17341 tttgtttgta ttggcgtggt taaaaatcgg gaagaagttt acaattttca cttttatcac
    17401 agtcactttg acctcccttt gtatccatat tttaccggaa acagtcctat ctaccgatcc
    17461 aatcatctgt gcgttgtttg ggggggctgt gatgggacta ggtattgggc tagcgttgag
    17521 aaacggccta tcatcgggcg gtctagatat cgtgagtatc acgattcgta aaaaaacagg
    17581 aaagtcgatt ggttctattt cgatctactt caatgcattg atcgtttttg ttgccggaat
    17641 tctatttggc tggcaatata tgttttacag tacgttgtct attttcgtca gtggaaaagt
    17701 aactgatgtc gtttatacta aacaaaagaa gatgcaggta atgatcgtaa cgaaacatcc
    17761 agaaaaagta gtaaatgaaa tccaaaactg tatgcgtaga gggatcacga tcatcaatga
    17821 agcagaaggt gcttataagc atgataagca gacagtattg atgacgatcg ttacacgata
    17881 cgaactgcct ctacttagag aagcaatgaa agcttctgat ccacaagctt ttgtaagtat
    17941 tgcagaaaac gtccagatac ttggtcgttt ctatgaggat ggcatttaga tcgagtttga
    18001 caaaaattca tgaaatactg ctgtagacag acgttcgttt gtctgcagtt tttttctacc
    18061 atagtagaat cagcaaagaa aaactgcttt tttatttgaa attttcttaa agttgaaaaa
    18121 aagcatgttt atttttttaa atttatggga tgatagaaaa aaatagtaga gaaaggacac
    18181 gattctactt gctattttca cttaataccc gtatattacg aatgaaaaaa gtttatgaaa
    18241 agttggaata ttgaatggaa aaacaaaaaa ggttaaaaaa cagccgctac atcactgggt
    18301 ttgatgggct ccgttcatta gctgtggtgg gtgttatctt ctatcacctt ctgccaacta
    18361 gtatgaaagg cggctatttg ggcgttccga tttttttcgt cgtatctggt tatttgatca
    18421 cggatctgct gcggcaggaa tgggaacaaa atggaaaaat caatatttgg caattttatg
    18481 tcagaagaat gaagagactc tatccggcac ttgtttttat gcttgtgaca gcttcggctt
    18541 atatcacttt gtttcagcga gggctgttaa acaatcttcg aggagttgtg gtcagcagct
    18601 tactgtatgt caacaactgg tggcaaatca ataacggttt atcttatttc gatcgatttg
    18661 ctaatgagtc accgtttacg catatctggt ctttagcagt agaaggtcaa aactatttgg
    18721 tttggccgat cttgtttgta ttgttgatgg tatttgttcg taaaagaaaa tggatctttt
    18781 atactgtgtt aggtacatcc ttcctctccg ccatcttgat gatgatcttg tatacaccag
    18841 gaggcgatcc aacccgtgtt tattacggta ccgacactcg tctgttttct atatggttgg
    18901 gaagtgcgct tgcgtttgtt tggccaagta cgcgtttaaa gaaaaacatc ccgaaacaag
    18961 cgaaaaatat tctgaatatc gctggaggaa tctcgctttt ggcactgatt ctttgcttct
    19021 tctttttaga tgatcattac aatttcatct attacggagg aatgctccta gtgagtctgt
    19081 tatgcgtagt gctagttgca gtgacagcac accccggagc aagtttgaat cgatggctga
    19141 caaatccagt atttacttgg attggcaaac gaagctatgg catttatctg tatcagtttc
    19201 cagttatgat tttttatgaa gcaaaggtga ctgatattgc cgatcatgtt ttgttgcata
    19261 ctttagtgga aatcacattg attctaatca tcagtgaact ttcttatcga tatgtggagc
    19321 gtccgctagc acgttttgat tatggtcgta cattccaagt gatgaaagga tggttcagca
    19381 aaccgatcat cagtaagagc aaaccatggg ttgttccagg cacattactt gtagcaattg
    19441 cactcgttgg tttcgttacg gcaccgaaaa acgctgtaac agccgatcaa aaacaactgc
    19501 aggcaaaaat cgctgaaagt aaaaaattag cagaagaaac cagaaacaat tcagaaaaca
    19561 ccactagttc ggttgaccaa gcgatccttg ataagtacga gttgtccaag gcacaaggga
    19621 ataaggcaca aaaattagaa ctaacggcat ttggtgattc agttatgttg gatgctgcaa
    19681 cggatctaca agaactttat ccaaaagtag tggtagatgg agatgtcgga cgacaattat
    19741 atgccagtgt accgtacatt gaaaaattaa aggaacaaga tttgttgaaa gacactgttc
    19801 tgatcggact aggaacgaat ggctcattct cggaagctca gtttgatagt gtgatggatg
    19861 caatcggtga tcgaaaagta tactgggtca atgtccgagt accgacgaag cgctggcaaa
    19921 atgacgtgaa tgcgatgctt gaacagatga cgaaaaaata cgacaatatg acgctgattg
    19981 attggtacag ctacagtaat gaccatagtg actggtttta tgatgatcaa gtccatccaa
    20041 atcctgaagg gatgacacat tacattcact tagtcagcga agccttgcta ggaggaagtt
    20101 caagtacgaa cagcagtgag acaaacgtga cagaatgata gctacaaaaa acttcaggca
    20161 aaaacatttg tttttgcctg aagttttttt atatttaccg tgtttttaac attcatatag
    20221 attcaggaag caaaacggtt gagtgtgtct gtaaataacg ttctttttgt tttcgggtga
    20281 gttttttgaa tgcagcttct gcttttgttg cttcgcttct tgaagaaaat acttctgcat
    20341 ggatcatctt aacaggtctt cttttttgaa gacgagtata tttagcacca gtaccattgt
    20401 tatgttcctc taatcgtcta gaaagatcgg ttgtatatcc accataaaat gtctgatctt
    20461 tacagaataa tacgtaaaaa taatgttcat ttgccataga gcatctcacg aatctctgca
    20521 gtgtattctc catccatatt gtaagtgatc aatggtggag cgactttgaa tccgtctaat
    20581 ttgccttctt tgattccttc aactaacaaa atatttgcct cttttccagg ttttgggtaa
    20641 acaaattgga tccttttagg tgcaattcga tagtcttcca tgacctgaat gatttctaag
    20701 aaccgatcag gacgatggac catagtaaag cgtccatttg tttttagtag cttacttgaa
    20761 atatcgatca cttcttctaa cgtcgtatga atctcatgac gggcgattgc caggtgttta
    20821 ttagggttcg ttttatttgt tggcagccct ttgaaataag gtggattaca gacgactaag
    20881 tcacaagaat tatgtcgaac gacagataag ctgtctttca gatcgatggt gtggacagtc
    20941 atttgttcat ttaaatggtt aagcgcgata ctacgtcttg ccatgtctgc tagacgaggt
    21001 tgaagttcga tagcgtcgat agccgcctgg gttcgtttac ttaaaaaaag tccaactgcg
    21061 ccatttcctg cacaaaggtc aacaatcttc ccttttttag gtacacggga aaagtgagca
    21121 agcagcacag catctaaaga aaaagaaaac actttggaac tttggataat cttgatatct
    21181 tctgcgtata attggtcgat tcgttcgtct tcgtgaagca tggattccct ccaaaatgaa
    21241 aaaaatagtc gtctgtatta tactattttt attaaaaaga accaaggtgg cttgtcaaac
    21301 ctctattttt tatgcatact atgaccataa gaagggaaag gaagagacta tgttttatcg
    21361 atttatgcgc ggggtagttc gagtactatt agccttagtt aatggaaatg cccgctatga
    21421 gaataaagaa gttttaccgc aagatgagaa ttacatacta gttgctcccc atcgtacttg
    21481 gtgggaccca ttatatctgg cggtagctgc agcacccaag gaattcagct ttatggcaaa
    21541 agaagaactg ttcaaaaatc cagttctgcg atttatatta actcgatcca atgcttttcc
    21601 agttaaaaga gaaaatccag gaccaagcgt cattaaaaca cctgtaaaga ttttgaaatc
    21661 gacaaatttg agtttgatca tgtttcccag tgggacaaga catgctacag aacttaaagg
    21721 cggaatggca ttgatttcca gaatgtctaa agcgaaaatc gtgccagctg tatatcaagg
    21781 tccgttgaca cttggggatc tgttcaagcg tcagagagtg acagtgcgtt tcggcgaacc
    21841 aatcgactta tcagatatca agaaaacgga taaagaaggt atggcggaga tcgaacgacg
    21901 aacacaagca gcttttgaca aattggacaa agaagtgaac cctgatttca aatatgaagt
    21961 caaaaaaaaa taaaatgata agtttatgcg tgggcggtct tttgaatcta ctgtcctgtt
    22021 cagttcagac tttttcaatg aaaacgaggg agagacgtca agtctgccct ctttttgcgt
    22081 tccaatatgt aaaatagaag acaggtgtca tgtaatggat caaagtgtta taataattag
    22141 aaggacattt tttgaggtga ttgaatgcgt tttttacata cagccgattg gcatatcgga
    22201 aaaaaactac aaggttatga cttgattgaa gaccagcgtc atgtactatc ttctatacta
    22261 gatatcgcga aagaagaaaa agtagaggca atcgtgattg ccggagactt atatgatagg
    22321 agtgtcccag ctgttgatgc cgtagaatta tacaatgaat tgatggtcga ttggaattta
    22381 aaggaaaaga tcccgatttt tgctatatca ggaaatcacg atagcagtac gagactttca
    22441 gcgggtacgc catggttcag ttattcgaac tattttttgc gaacacagct agcagaagca
    22501 tttgaaccag tcgagtttaa aaacgtacaa tttttctttc ttccttactt tgaaccaatt
    22561 gcagcgagat tatattttga agatgacaat atccgaacga tcaagcaagc ggtagccaag
    22621 gtgatagata aaatgcagga gctatttgat tcagaaaaaa aacaagtatt gattagccat
    22681 ttttttgtaa tgggaagtgt aaaatctgat tcggaaacga aaatcgaagt aggcgggttg
    22741 gattcgatcc ctggtgaact acttacaccg tttgattatg tagcactagg acatttgcat
    22801 aatcaagctg ctttgagaca ggataatgct cgctatagcg gttccccatt gaaattctct
    22861 ttatctgaga tcaaccaaaa aaaaggcgta tggatcgttg acctgacaac aggcctggag
    22921 ttgaatttta gggagatcga acccctgcat gatattgaag aaataacagc gagtttcaaa
    22981 gaattgctgg atcctgcctt ttatcagtcc gtcaatcggg agaattacat ctatatctat
    23041 ctcactgata gaacgatcat cccgaatatg atgaaccagc ttcggcaaat atatcctcgt
    23101 attttgggga ttgaacgggc aaacggtcga gcagaacaaa caaagcgcaa acgaaaagta
    23161 ctagaaatga aagatcctca aaaattagca gaagcatttt ttagtgaaat gacggaagag
    23221 cagctaactg caaaccaaga aaaatggctc ggagaaacgt tgcaagaaat catggaaatg
    23281 gaatgatcaa gaatgaaacc gaaaaaatta caacttaaaa attttggtcc ttttatcaat
    23341 gagaccgtag atttttcacg tttgacagaa gcaccgcttt ttttgatcag cgggaagaca
    23401 ggagctggca aaacgacgat ttttgatggg atcaccttcg cactttttgg tgaaacttcc
    23461 ggaagacttc gctctggaaa agagatgcgt tctttgtttg ctacaccaga cgaagaaaca
    23521 agcgtgactt tttcttttga acatcagcag atgagctatg aaatcgaacg aaaaccagaa
    23581 caagttttag caaaacgaaa aggaaacgga acaaaaaaac aggcagccaa agtaacgttg
    23641 accatttttg atgggaaagg aaaagaaata aaacaaatca caaaacgtac agaagcagat
    23701 cagttgatca gagaactgat gaatctcgat gcgaaacagt tttctcaaat cgttttacta
    23761 ccccaaggag aattccgtaa ttttttggtt tcatcaagca gtgataaaga gactgtttta
    23821 cgtcatttat tcggtactca atttttccaa cagttcaatg agcgattaaa agaaaaagca
    23881 aaacaacagc aacaaaaatt ggatcattta gagcaagagc tgcatcttct gcaaaaaagg
    23941 tttgttttag tcaaagaaga ggaaccagca gcagggtttg aagccgtttt agcccaatgg
    24001 acaaaccatc aggcaatact cgaaaaacaa atcacggaag aaaaagaagc tctaacggaa
    24061 cttcaggaaa aacaaaagca actagaaaat tcctactatc gttttgaaag cttgaaaaaa
    24121 agttatgaag aaaaaacgat attaataaac aagcaaaagg aattagctgc tcaaaaagac
    24181 gaaatagagg aaaagaaacg atggattggg tattatgaat ttacgcaacg attagtcgaa
    24241 ccaatccagc gcctacaaga aataaaagtt gaaaaaatag aactgtcaca aaaaagtagt
    24301 gagcaaacaa agaaactagc gatgactacg cagaattatg aagagtggca aaaaaatgaa
    24361 gcaaaccgcg tacgccgaca aacaaaaatc aaagaagctg ctcaaaaact gcaaaacaaa
    24421 cagatgatgg tgccaattgt cgaatcctta cgtagtaaaa aacaagacgc agaacaatta
    24481 acaatttcat tggataaaga agaattgaat gccgagaagc tgtatcaaaa tctcgctatc
    24541 tatcaggaaa gaactcgaat tcttcaagaa aatttactgg atcaggaaaa actgcaagaa
    24601 gatcgtcttg cttttggaca gttaaaatat ctagatgaaa agcagcaaga aatagaaaaa
    24661 caaaaagcaa aagacactgg gtcgctcgca cagataagag aagaactcaa aaaagcaact
    24721 gaagaaataa caaaaaatag tcagctattg aaagaacaga cagaacaagc agaacagctg
    24781 aaaagccaat gggcaaagca gcaaattgct cgtcttcagc tttttttgcg tccaggggag
    24841 ccatgtcttg tttgtggatc aacggaacac cccatacaac aattaaaaca cgaacagcca
    24901 aataaggaag atatcttaaa agtagaacaa caactggcag aagctgagtc tgctgtagaa
    24961 agaactcagc aaatcattgc caaaagtgaa gcacatgaaa ataatcttca acaacaatta
    25021 tcagatttag aacaaaaaat catggcaacg aatatacaat tggctgaaca agaagaacac
    25081 ttgcaacagg ctttgaaaaa ggcatttcct gagataacag aacaaaaaac aaaaacaact
    25141 cttgtagtaa tcgaacaatc tttagataga cgaaaaagca agatggccaa tgcaagaata
    25201 gagctggaag aaataagaca acaagcagaa aagctcgaac aacaattagc tgtctacaaa
    25261 cagcagcaga cagaaaaaaa taatcagctc cagcaactgc aaggagaaat caaaagttta
    25321 gaacaacaat tgcaagatac ggatagtaac tgtttagaag aggagatacg acgacttgaa
    25381 aaacagttag aagaagactc gaatcaacta gctgttgagc agaaagcagg agtgcaattg
    25441 ttgcaagatc ttcatacgct tcggacacag cttgatcttc aacatgaaca aatagaaaaa
    25501 atagatgaaa aaatagaaaa ggaacaagaa agaatagcta aaaagttaga aagccagtcg
    25561 tatttttcaa caattgaaga aatctgtgca tttgcacaac agcaagcaca atataaagaa
    25621 aaccaacgta cagttacttc ttatcatgaa gagcaattag tgacagctga ccggctgcgt
    25681 caattagaaa atatccagct aactgagaca ttcccagata cagaagagat cagccaacag
    25741 ttagaagaaa taagacgtat gatcgaaata aagcaagaaa agatatacgg tttacaagaa
    25801 caaaaaatta gtaatcaaaa aatctacgat gaatgtcttt cgatttatca cacaagccag
    25861 acgaaattag atgagcttac gcaattacag cagctttcgc aaacattcaa cggagaaaat
    25921 cctaagaaga ctagtttgga acggtatgtg cttcaggttt atcttcaaga agtgttacaa
    25981 gtagcaaatg atcatctcca acgtttaacg aaaaatcgtt atcaatttga gctagcggat
    26041 accattggca gttatcgagg gaaaaccgga ttagaaatca atatctatga tgatcaggca
    26101 ggtatgtctc gaagtgcgca taccctgtct ggcggtgaga gttttattgc agcactttct
    26161 ttagcattag ctcttgccga tgtgatccag acacaagctg gtggtgtagc aatcgatgca
    26221 ttgtttattg atgaaggatt tgggtcatta gatgaagaag ctttggaaat ggcaatggaa
    26281 gctttagaaa cgatcgaaag tgaaggaaga atgatcggca tcattagtca tgtaagagaa
    26341 ttgaaagaac gagtattaca acaaataaga attgatacag aaggtagcgg acaaagcaaa
    26401 atacgctatc aacttggata aaaagggaga ttggtaacta tgaaatggag tattccagaa
    26461 aaagtcattg cacgtggacg tcaatacttg caagaaggcc gagtgttatc ggttgtccct
    26521 gaccaagaag aaaacgtgtg gcatgcagaa gtcttgggca gtgaattgta tatggttgat
    26581 ttggatggta cagcaaaaga gatagattac tgccaatgtc cctattggga ggaacataaa
    26641 tattgtaagc atacagttgc tgtggaactt tatttacggt cgaaaaatct atctcgtatc
    26701 atgaaaaaag accagccaat gctagaaccg gtaaaagcaa caagtgaggg agaaatcatt
    26761 gcaaaaggtt tttctcgtct aaaaacacaa aacaatcctc aagcggtgca acctttgatt
    26821 attgaatacc aagtagaaac gattgagact aatcagtatc acccagagct ttctattttg
    26881 gcactttact tgcgtatcgg ttcattggca tcaccgaaaa aaacatatat cgtaaaaaac
    26941 atctatgatt ttttacaagc ttacacagaa aaagaaactt acactgttaa taaacagtac
    27001 cagtttcgtc tagataaacg ggcgtttcaa aaagaagatc aaaaaatact cacgcatctt
    27061 gcaggaagtg ctcagacgca tcaattatta ggtacgaacg gcttacaagt caaaggaaaa
    27121 ctagataaaa gatatctgct tctcccagta gaacaagccc gcttcctgtt agagcagatg
    27181 aatcagacta cacgtttgca tctgcagata aatgaacaaa acgatcaagg aatacaattt
    27241 tccgatcaaa acaaaccttt atcattcaaa gtggaaaaat ttggagaaga ttatttgctg
    27301 caagcattga atgattttga ttttttcttt atgcattatc agtggggagt tatccaaggg
    27361 acattttatt ctctgacgaa atatcaacaa acggtttatc aaacttggaa acaattgatt
    27421 cgccgattgg agaaaccaga agtagtgttc aagaaaaaag aattaccgtc tttgtttaaa
    27481 gagattatcc ctctgctgtc agagatcggc gacgtagcta ttgcccctgc agtagaagag
    27541 gaagtagcag atatccccgt cgaatttatt ttctttttcc gaaaagcaaa aggaaaaatc
    27601 caagctcgag tcgatttcgt ctatggcgaa gtcatctatt cgactgaccc gaagcatgag
    27661 gtctcgacag aacaggcttt tcaggtgtta cgtgacacag tagaagagaa acgagtactt
    27721 gatctattca gaatgtatcg ctaccagaaa aatgaaactg gttatgaacg tccattgcca
    27781 agcggggaag aactatatgc ctttttccgc acagaactat ctgtcttccg tcagtttggt
    27841 gaagtcagac ttggaagaaa attgcgagag ttgtatttag atgcgcataa acatcaaccg
    27901 aaaatcgaag taaccgagac cggttcatgg ctcgatattc gattcgatgt gacagggata
    27961 gaggagcaag aaatcgatca tgtcctgcaa agcttgctga gaaatgatgc tttttataca
    28021 ttagaaaatg gtcaggttct ttcttttgat tcagaagaat ttcaacaaac aagccaagtg
    28081 ttgcaacaat tacgggaatc gatacgtaca gaagaaggaa cgatccatgt acctaaaaat
    28141 caaggattga tcattcaaaa tcaattggaa aaaagcaatg ctacttttag tgaatcgttc
    28201 caaacgatgg tacaagatct cattcatcca gaaagatacc aggcacagct tcccaaagga
    28261 ttgaatgcaa cgatgagaga ttatcaaaag caaggttttc gatggttgaa gatgttaggt
    28321 cattatcagt ttggcggtat tttagccgat gaaatgggac ttgggaaaac actacagaca
    28381 attgcctttt tactttcgga aaaagaagaa cggaaaagct tctctgcttt gatcgtagcg
    28441 cctgcgagct taatttacaa ctggcaggca gaggtgagaa aattcgcccc ttctttaagt
    28501 atccaagtaa tcaatgggaa taaaaaagaa cgtgaagaat tactggctaa agacacagat
    28561 atccgagtga cttcttatgc cagcttaaga caggatctag ctaattatca gtcgcaaaaa
    28621 attgattatt tgattcttga tgaggcacaa atggtcaaaa atagcagtac gaaaactgca
    28681 caagcattga gagaattagc tgtacctcag cggtttgcac tgagtgggac accaatcgag
    28741 aataatttag aagaattgtg gtcattgttt gcaacgatta tgccaggatt tttcccgact
    28801 aaaacaaaat tcagggaact tgccgcagaa gaaattgcgc aaatgattcg accgtttatt
    28861 ttacggcgag ataaacaaac tgtgttgaaa gacttgccgg aaaaaacaga gatgaatcta
    28921 tattctgcac ttacagaaga acaaaaaact gtttatttag cttacttgcg tcaaatgcgt
    28981 gaagaaatca catcaatgga ttctgaagca ttcaagaaaa accggatcgg gatacttgct
    29041 ggtctgacaa gactgcgaca aatttgctgc gatcctcgtc tgtttattga agattaccaa
    29101 ggcggttccg gcaagctcga acaagtaaaa gatctactgg tagctgccaa agagaacaaa
    29161 cgaagagttt tgctgttttc tcaatttaca gggatgctga ctatcttgca agaagagtta
    29221 gccgagctag ggatctcgac attttattta agaggcagta cgaaaccaca agatcggtta
    29281 agtatggtag acgcatttaa tgctggcgaa aaagatgttt ttctgatttc cttaaaggct
    29341 gggggaactg gattgaatct cacgggagca gatacggtca tcttgtatga cctttggtgg
    29401 aatcctgcca ttgaagaaca agctgctgga cgtgcacatc ggatcggaca aaaaaatgtg
    29461 gtagaagtgt ggcgtatgat ctctgaagga acgattgagg aacgcatgga ttcactccag
    29521 caagaaaaac gcgaactgtt ccaaaaagtg atccaaggta atgaagaaca gttgacgaaa
    29581 ctgactgaag aagacatccg catgatttta agtgtaggag aaatcgagga gtaagagact
    29641 cgaaaaagcg ttcagtaatg ggtaagaaag cctttctaaa gaagcgattg tttatttgat
    29701 ttaaaaaata gcaaggaggc tgggattcca ttttatccca gcttccttgc tatcattttt
    29761 ttatttctat ttttaaaggt tatacaaaga ttgtcttcat tgtctctaac agctgatcaa
    29821 aagaagaaat ctcgatatcg caaattggag tctgttcaat tttgcgttct cgatggttga
    29881 accaaagcgc ttgccagtta gcactttttg cacctagaac atcattgtcg taattatcgc
    29941 caacgtataa ggtctcttct ggaagcatat gaaattcttt ctcagctagc tgaaaaatct
    30001 ctttttctgg tttttggaaa cctgttgctt gagaaatgag catattgcgg gaaggaatcc
    30061 aattgttcaa ttgaagttgc ttcagttttt ttgtctgatg gtcagtcggc ccatttgtga
    30121 taattcctaa tgggacattc ttttccttca agaaatcgag ggtttttttg acctcctcat
    30181 gcaagcagat attatcaagt tcttcctcgt aaattttttg aaataagagg ccatcttctt
    30241 ccgtaatatg agggtagtct aaatctttta gtgattggct gatccgatgt gctcgcatat
    30301 attctagtgt ccaatctcct gccattactt ttgggaagtt ttcatcacta tgatggcgga
    30361 accggatata tagtggatgc atatctgcat ctgaaacaag cggaacgact cttttgaccg
    30421 cgtttcgaaa tggctgctgc tgatcataca tcgtatcatc aacatcaaac acaactgatt
    30481 taatcaaaat tttacacgcc tttcggtttt cattgatttt ttcacaaatc cttgactatt
    30541 gtaacagaaa aaattgtcta aaaaagctga taaataaagg tttttcttac tatatttcta
    30601 tttccttgac agaataaggt ttttatattt cagttttttt cactaaaaat cgtgaaacac
    30661 gtcaataagt ttgatgaaca aattaatcat tatggtagaa gtcgattgat ttattttaat
    30721 gcaatctctg tggaattttc ga
//
LOCUS       DACESX010000018        30701 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.018, whole genome shotgun sequence.
ACCESSION   DACESX010000018 DACESX010000000
VERSION     DACESX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.018"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(490..1677)
                     /locus_tag="IXF88_000702"
     CDS             complement(490..1677)
                     /locus_tag="IXF88_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1507450.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            2396..3199
                     /locus_tag="IXF88_000703"
     CDS             2396..3199
                     /locus_tag="IXF88_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1507451.1"
                     /translation="MSQEVTENVIDNDVDLVSDQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNVFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQQETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFESFDYEKIYAKKGRKVDRLRFTFSEPESNLPSISMHNWLEED"
     gene            3806..4249
                     /locus_tag="IXF88_000704"
     CDS             3806..4249
                     /locus_tag="IXF88_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507452.1"
                     /translation="MDAINWHEFVNYMEKLLDDLKSFSIADAVKHGLPVSTTMKMRGL
                     KVPDEIIKLRTKTIIETAKIVDSMNLDLKGKKTTYEKDFVRFLKNVIGKYLGKKSFTE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIVEYLENKEQKKGR"
     gene            4368..5426
                     /locus_tag="IXF88_000705"
     CDS             4368..5426
                     /locus_tag="IXF88_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAQ1507453.1"
                     /translation="MEKDVNSASTKDQYNIIGGLLGDKDKRTRHGLRIMLATFLGVFL
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLTFARSGAEITISDVWTDKTRDVTVVKLG
                     YSGNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDYTADDDTKVNVNESDSVTKALSQYSMNDVDNEGVISFGKKAKPVN
                     TPDNINFRINPYSDTTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKTINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            5468..7417
                     /locus_tag="IXF88_000706"
     CDS             5468..7417
                     /locus_tag="IXF88_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1507454.1"
                     /translation="MENSTEFFKNIQTEFNHALTLMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAKRQRQEGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TIIPRLKEYERILKSEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPEASLK
                     LSKKIPDELLEEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDIAKLEEELKSIKGQPVAVYTVTMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRKLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVNDTVSLSNTILFATTNAGAE
                     MYDSMNRYSSLSRVANQENSNEYSTELKREYRKVALEIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAHNEILKSAELVRKHLNVDLRLPPQKKHFYGQEFYADDLSMHLV
                     FDRASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSKAGGFEGKGDT
                     DAEIIAKPSIAESKENKFVFSVGGV"
     gene            7420..11109
                     /locus_tag="IXF88_000707"
     CDS             7420..11109
                     /locus_tag="IXF88_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507455.1"
                     /translation="MKFKKSLLQNRKIKRWVVRGLVIVGALAVTLGAPFGMNDPTSDD
                     QGNKQETVANTNVAYAESKPAEKGVWTLYARILMGDKEVNDSATKTGQMNLLGQGGIS
                     ADFPYSDISKQGNAIAGGNAGTQLASTLATYNYYGYIETVSGNAIASQASSFIGGIGR
                     TLGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGKGGSDVSNPVAKALTSLL
                     NNLGLNNQLFKAIASLSMLFFTAILGYQGMKLLSKEGVNSKGLSPVLEKWIVRIFVLF
                     VFLPVAGSLLGVYINGIQSAKEDGKLPTNVVGDYIINTRLWASTQNLAPSSDTGGNVP
                     NGKPTSEIKFVDTDYEPSKKSEMIRMINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NVNTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGNAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTTNNFDATQPWGVANNATFSTQSVALMLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFVRVWEVVLTGSLAAGAGVMAISLGVVTTSLIGLQLPIFLITFVRAFGDTAV
                     GFIPNTLLGDGVANIVRSAMVLGVGYLLVFKKGEKVNNNLINMLIGLPLEVAFGFDER
                     AKLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGRGSVKDMVAGGLGAVGAQGIAD
                     RLNNMGDSNADAKTIADKVVPETPASVPFNGGGVSGTINNTSFKDLVRQDQLRNNSQQ
                     YSDHLGRMVDEKGNFVDENGEVLKDSKFGKDAKGNFIDDKGRLVDEDGNLIDTNGKPL
                     EDNEDGNIGMDSQGRLIDDKGRLVDDEGNLVDENGRKLLNGDFGQDVNGRLVDDKGRL
                     VDEDGNLVDEFGKKLGKHSNGYNVNGDTVDEDGNIIDSRDALVADRNKELEQQQHTPI
                     AVAIPDDESDEVILDQAHQGLREEAKDKVVSDQAQEELKNKSNGEDVSNQYHQTAGNV
                     PVEGQSNELHNNFDNVADRNDTVLKEKESLRQETDKDQSKLKKTTIPTPIGSTVPKVN
                     QANDSETNTGLGMAKDNQSIREPKKTTESSKSNKSKSTSNKTGKSKEPSKAKSAVEYL
                     SKTGKNVYTSADKALAKAPKGSLAGMTYEAKNEAIKLGKGTAKVAKVTAKGATKTGKA
                     VKKSTMSVKDYIAKYSEQKPTDKKS"
     gene            11521..11859
                     /locus_tag="IXF88_000708"
     CDS             11521..11859
                     /locus_tag="IXF88_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507456.1"
                     /translation="MFDSIKNLVSGMTPEMKTLVQAILYLMATFFALKPGFTAMKHFG
                     DAKWGEGLKYVGAVVAIFIIPVITTVMLLTMGTKTGNDLNQKANMISALIPVVGIYLV
                     TKFPKEEQLG"
     gene            11971..12207
                     /locus_tag="IXF88_000709"
     CDS             11971..12207
                     /locus_tag="IXF88_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507457.1"
                     /translation="MDREKMLVHANEVLTSGFKGTDLAKIMNMNVNQFYDYRNGNKKI
                     EKARLETLIKFEKAYLYVLDKQKRTIDRKKGVLQ"
     gene            12207..14372
                     /locus_tag="IXF88_000710"
     CDS             12207..14372
                     /locus_tag="IXF88_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507458.1"
                     /translation="MAKFKDITESINYVAETVPVVVLSLKPQLLEVYQKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYVVIEDVQKLSSEEFPDEEDAILDSEKYVVLLKSSLN
                     YVVDLPEKFKEKLLAGVRFMDSNGQLLVIPDDPNMFEREVEEATDFYSAVDQLEVATK
                     GLERIAETFNSSDAVEFEAVAEAETEAVPDEEEALFEDNDEATEAGNQEASIEETQTE
                     TYQQPKIDEKPESQSDKENYVYEAEKIVSESDVPKNLDADLLEKDFEQIIANKFQPVV
                     LDEVSDPVDLNEEMRSIAELKPLLFLAKESMNARISIANNKINSELNKTKEQLLLKGK
                     QMLADELLRIEEATSLSGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMA
                     KQAYLEAELKKLEQRYDAEHLPEVKQQEDEFRKATEESLDQQYQEAIESIKNDALKQN
                     QEAKEVVVERIINSLKPEIEKSLTEVQEKTLEIVEKTKAENEAEFHDTKEKTTDVVVK
                     YFANKEEVEKLVAKDTNELESKLVAQQNIIEDYKNRVKEFKDLADGYKLNSQKLTEQS
                     EALREDLSRARNDLSQMAEKTKSLVVTTENQTESKRDFTSKKMATIIAVSAMACGTIL
                     GSSYFISHNHQEAQAQTETSISRVENSLGSNTASTSTTDNNYEATPTTEFKVGEDVVI
                     SIDGKTTKAEVLSIDGDKAVVKAPDGNQYKIQAHLTEKN"
     gene            14384..14614
                     /locus_tag="IXF88_000711"
     CDS             14384..14614
                     /locus_tag="IXF88_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1507459.1"
                     /translation="MREENIVYPVIENQANTKSSQFLQEVQVVYPVNELPQTNETVSK
                     SISLIGLLIVATALTVGVYRKLKVKGSISDGR"
     gene            14604..15470
                     /locus_tag="IXF88_000712"
     CDS             14604..15470
                     /locus_tag="IXF88_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507460.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVDWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLRFIFIPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNTARVTMGKKKPFRGTEILKVRQDGTIMYRDGSVALMFNLDGATSLTAFP
                     EEIIAQEGKSIQYQNARDRSLAEIKITSSQPQNTERQIRNMKVQKRNAKSNKAVFDIV
                     DQQERFVSEYIDGVKTTVIQYLILKSQSQMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSEIYALK"
     gene            15488..17446
                     /locus_tag="IXF88_000713"
     CDS             15488..17446
                     /locus_tag="IXF88_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507461.1"
                     /translation="MELMSRSKLLNFRKKKHKKEQNADSSKAVNNTYVSDLTSLVGYQ
                     FTPSYIRTGNRWGTIVKLVNTYGMNRNYEFGWMVKAIPEVKVEGVKAYLIEADKPVSQ
                     KDQQEIMHENVGGSTNSYENNLNDKNSDAEINKTVMRIIDLNTASLLDSTANKIIDWK
                     LEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSFGGDQRDLFDRLLSEPVGSVYEY
                     TSMSSDFAGNDHAIRKGLDDADGWAIGTMSTSYASGVAFMAMDRSFKGKVLVASHKDA
                     TIKGYREEISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGETFVAKPSLNSV
                     IEKINLAEEGLNSLELFGDRKDIGDLYRRNLDKLTQIFYLLADRELNPATRVLLKKEL
                     GNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKTGTEKEIDRII
                     VLKDTLRTALDSFSKMFNQRTTLKEIGKSDKLQFYYNLSDLRTEINVMEAQFLNTFDF
                     ITSQAKKGDIVMIHGFDRLSVETVGLIKKRLTMLEEDGVRLAFLFDTIGSGEDKDGIE
                     TADIFNTDGKIYQSFESDFNYTIIGKMTSEELMKYEKKVAPARKGEDDQKLSVDLRQY
                     LTSGRSNKFQIRRPVDLTNNFVIGEFII"
     gene            17585..17920
                     /locus_tag="IXF88_000714"
     CDS             17585..17920
                     /locus_tag="IXF88_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507462.1"
                     /translation="MKQYLNEWKVIEGSLVAQRIKGLPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDEKYFIIQEYEGQLTPFYTTWEMSEDGINNFPCESI
                     EESISQTEC"
     gene            complement(18446..20041)
                     /locus_tag="IXF88_000715"
     CDS             complement(18446..20041)
                     /locus_tag="IXF88_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507463.1"
                     /translation="MFILDDEINKVKNPRIVPLLEEVRSSFYQGNYRSAIAVNYSALV
                     LDLIDKLSDLANIYEDDTSKKILEEIAKLRKSDPNSPRWEMELLEKTYNSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGDTWELREITKETCQDVIRKSYEIVFLKNPILGKKV
                     TNEIIQYASRIHSMSVTPEEFHSALKNSYLKKLSEKAKENLCKTAFKFVFKLPYGNEE
                     VDRDRVSTFKLLDALIFDNKEFYLKILKKEISNYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIASHPELQRDFSEEIIQNLKISIKDFEPKQPITTHIEDYYRLRSLAVLGNT
                     KEELQEHLNRLLDPILQQNMRVSSLATSTLETIYNQYLYFGEKEMFLDFMIEHLTDSP
                     AFRYSDKDMEILPWIYDKLNQDQFNKLLYNLNRNNQFYYNGYFSSFISNLLDFYKQKF
                     HMDLRTHYNGLLYPNAVTMDQKFQLKDKELKKLYELAESKSDTYPEILNDLKLNLVNK
                     SEQYLGEKINSEEKLLDYIKQIK"
     gene            20162..20746
                     /locus_tag="IXF88_000716"
     CDS             20162..20746
                     /locus_tag="IXF88_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507464.1"
                     /translation="MGLKQSVYIWLYPDNPMSIMADIFVGSILLCLLYLIFFHNLIGY
                     MILDWKGMFDIERKPFNNKQIYTTFAGGDLEEKEVDVTHEIGLALSGIQDADVLGSMI
                     TDTGYNAYEVFYLMYFIWRDSQDIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     NTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            20757..21008
                     /locus_tag="IXF88_000717"
     CDS             20757..21008
                     /locus_tag="IXF88_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507465.1"
                     /translation="MKKIIDQPTNRLIVEGVIFVTMYFTVGNGISNTIIQKDPQYLLF
                     SLYAWIVQMLWVSLLMLLFYWIVRVILLVLNKAKKKKDR"
     gene            21015..21737
                     /locus_tag="IXF88_000718"
     CDS             21015..21737
                     /locus_tag="IXF88_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507466.1"
                     /translation="MKATKLKLVLFSTAVLLTLSACGTSKSADNTKDTKSSSTVVTES
                     SSAQTSTTMSSMTENNAEKFQKYKDVNQGKKPEEVYNEFISSVKNDESYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIQEGFSYAPTLMHDITIGKPAYEYNAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTLAVVTPKKIDGTLYWDLMGMTTNTSDEMIESTSNYYM
                     DLGAYKSYEQSEPVMDDQSTIK"
     gene            21867..22610
                     /locus_tag="IXF88_000719"
     CDS             21867..22610
                     /locus_tag="IXF88_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507467.1"
                     /translation="MKEISASEAHISQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQELVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            22616..22981
                     /locus_tag="IXF88_000720"
     CDS             22616..22981
                     /locus_tag="IXF88_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507468.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKQRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            22997..23527
                     /locus_tag="IXF88_000721"
     CDS             22997..23527
                     /locus_tag="IXF88_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507469.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            23689..24054
                     /locus_tag="IXF88_000722"
     CDS             23689..24054
                     /locus_tag="IXF88_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507470.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     RKLAQSYLEEKAGVIKDKE"
     gene            24071..24460
                     /locus_tag="IXF88_000723"
     CDS             24071..24460
                     /locus_tag="IXF88_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507471.1"
                     /translation="MNEETELFLKKVNFLVEKYFLTKHKIMYLIKGLIGGIILWILTF
                     FSLFGGFTNYKKLVDENVKQISASESLRHQAEDLVSNPLFIGGMVEVIIISFVIGYLG
                     MCIFQIIFDLIRGKVFQEFKSEKINLR"
     gene            24490..24714
                     /locus_tag="IXF88_000724"
     CDS             24490..24714
                     /locus_tag="IXF88_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507472.1"
                     /translation="MEQTNHITEETRKFICLESFYSEGRYCNKGETYTAYPIEGGFKL
                     VFENGDMNFTTELFECVLETWSDVLLEVTK"
     gene            24767..25048
                     /locus_tag="IXF88_000725"
     CDS             24767..25048
                     /locus_tag="IXF88_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507473.1"
                     /translation="MAYENLQLVVAGVSGDIYLAKAKDGIMDTNYRRVATNDVLVATT
                     EWFIRNNKKLIQFEADENGRHSLFYTNDQKKADRIIAILKENDDGGQRD"
     gene            25029..25271
                     /locus_tag="IXF88_000726"
     CDS             25029..25271
                     /locus_tag="IXF88_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507474.1"
                     /translation="MVDKEIKGEIRLFQRENDDWGTEISKNLAEDIELRLSFISMIIE
                     SFAFELGIPQEKLAEILIKASNNSPQHFHIDLGTVN"
     gene            25393..25641
                     /locus_tag="IXF88_000727"
     CDS             25393..25641
                     /locus_tag="IXF88_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507475.1"
                     /translation="MSENHLKRLSKGTASMFDANLLIFKSLLEFEKQRVEDIAKYGNY
                     ATVPSVYNDLMKWFKVQTGLEYSQQNLVVKIEEYENHG"
     gene            25916..26128
                     /locus_tag="IXF88_000728"
     CDS             25916..26128
                     /locus_tag="IXF88_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507476.1"
                     /translation="MPVKTNTEWLDICDELFDTKDKKELAMRVAYAIVQKDKYFAVPS
                     TDYDIEVIVDPITGDKKLRVVIECNA"
     gene            26237..26413
                     /locus_tag="IXF88_000729"
     CDS             26237..26413
                     /locus_tag="IXF88_000729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507477.1"
                     /translation="MDIFEVKPDSGKFIVLQEKRVIAIERTEIEAYKKIHELAEQYIE
                     DLNNNFQEFLTQNE"
     gene            26524..26745
                     /locus_tag="IXF88_000730"
     CDS             26524..26745
                     /locus_tag="IXF88_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507478.1"
                     /translation="MDIYEEVICDVEGKEEFLPCIKELETTVAITKVANAVSNETNEL
                     VGIHEAGNLVFSCLNSNKSALLALDGDLF"
     gene            27117..29357
                     /locus_tag="IXF88_000731"
     CDS             27117..29357
                     /locus_tag="IXF88_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507479.1"
                     /translation="MSEFEFLDKSIGSAFLIDPDLFGAWAQSSGYNWAFSDDMVELLS
                     QTMNWEAYKIKQFLDVVAQPIYTSADDHWSGRELTTLKELAEYNKRYKIPFQVSYMSD
                     DFSVIYSIGKESRYLIPNRHLEIKMNSDYTDYTLSELKALRQSDSEEFSALKNEVSAD
                     NFRRSDIEELKKKQKQQLEKQKKLIEDIEYKRVEELAIQEAKIEKMRQELYKKQQSML
                     AEANKMRFELEKKVEDLNTKIFYLETEINAIEGYFGESTTFVQLRSGQKSLPEEPVVM
                     FQKVRYLDEELGRYFSMREVEGTADTMLTFEQALSSRDDLFDLFSPNKKSISMIRISK
                     SGYGYTNGQMESTNMLEKYKKYHGETLAILLRNGDNLWICWLDENKISFKDDLFYQNK
                     VTEQASETKNMVKEIDWTHTRNLEERLKDAKKQKKAVRGEFASRYILFSILQGVLDNG
                     TMMTLPEKVNVIQAINQKSPYFIASVADGWLEDTRLPSFSELIDKYSFYKETGEDRVL
                     EAEYELNPDKFENMRDYPHKQNDDIYVLYRLRGWSPTSANYTYYNSDNRSNSESGRDL
                     AYSIDLKDGIYQINLVKQGKFQKQMFSRTFVGVDSTTGENVYDYYYERETEYYIRGQK
                     AYNKSDYWGDKAKRLTANFRVYESEFINLTFLNSVFIDYVITTKRIGSYSSTYASLVP
                     MLNSMSRFLKEREEKELEYIKEIMPDFEMHEWQIALSDWKLTNKVRRITPFQAKRFLK
                     AMKFVQ"
     gene            29495..30292
                     /locus_tag="IXF88_000732"
     CDS             29495..30292
                     /locus_tag="IXF88_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507480.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYGESQNKPRKKKQAYVLKMVNGE
                     ELIVLGYSKEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYNSNRFDRLEKFCRPYTVLKTKKFNKTKRVS
                     IKLPTGQISLGEKDYLIKVGKELIIKVPERLFKENFEIRETLVWKEE"
     gene            30332..30514
                     /locus_tag="IXF88_000733"
     CDS             30332..30514
                     /locus_tag="IXF88_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507481.1"
                     /translation="MAVNRKLQVLITDDFGGAEWFDVQSIDFEEEVVVIENVYGEEYS
                     YSLEGENVEDLRLIGE"
BASE COUNT        11226 a         4372 c         6090 g         9013 t
ORIGIN      
        1 aaattcacaa aattaaaaaa caaatatttt tttagttttt agaaataaat tcacatactt
       61 tgtctttttt tgtatatatt cggaatacca catcgctttt tgtccacatt cacatcgctt
      121 tttgtccatg ttcgcatcgc tttatgtcca ccctaaaatc tatttaagat tgatataaag
      181 cctttttgga gattgctaaa gttttaaaga ttaaaatata tattataaat ataaataaaa
      241 ggggaagctg aagcttcctc cttttccttt tttttaatta ttcaaaatta cacaagcaaa
      301 ataaactgct agatagtgtt agttttggtt ttaaggtttt taatttttga gattttgaaa
      361 taagagagac taactatata gagtcaagta aaaattagcc aaatcaaaaa atattttata
      421 acagataatt ccgaaaaaag ggtacaacaa ataagcttct taccattagt ttttttgaat
      481 gttcaatagc taatcagaaa caattatata aggctcgttt ttagtcaaaa tagcatgaat
      541 cacgtaacaa agcttacgag aaaccgcacc taaggcagtt agatgatgtt ttccttcgga
      601 aacttttttc tggtaaaacg cactaaaaac aggatcacat tgagaagcta ctaaagccgc
      661 cgaaaacaag gcttttcgca aatatggaga ccctcgttta ctcataacat tatgcgttgc
      721 ctcatattgc cctgattgtg tcacggaagc gtctatccca gcgtaagcaa cgagtttttt
      781 cgggttagaa aatttttgaa tatctccgat ctcaccaaga atagtcgcac cgtttatagg
      841 accaattcca ggaattgtga gaataacaga atttaaagtg tccattatgt gcttgatttc
      901 tctttccgtt tcctttattt ggctttcaat gaattttaat tgttcaatca ttgaacgtag
      961 ttggaaggtg aacgcttctt gagcaaattt tactccaaag gagttagaag ctgcttgctt
     1021 caattggtcc gctttttctg ccttgattcg gttcctgctt aactcagcta agtattgggc
     1081 caaagtttct gaggaaatgt tttctaagtc gatcggggca ctaaacgcca gcaataactc
     1141 tttagaagct tttccgaaaa cgccctgctt ggtaaaaatc gtcgcgtatt ctggaaagac
     1201 ttgatccaat acggctataa tttttctttt gaagtcgctc gctgtaccta ctaaataagt
     1261 tcggtaacga gaaagttgtt tcaaagaaaa aacattttca tcggataaga ccgtttcgac
     1321 aaatgagcca tagcgcatta agtcagcaat caaaacagaa tcaataatat cgttttttcg
     1381 tttccgtatt tctgtacctt tacgccatcc gtctgtttga aggggattca caacgtgaat
     1441 caaataatct gattcgtgaa ggtatgaaaa gatggacagc caatagtgcc ccgtggcttc
     1501 catccctata acaaagtcac tgggtgctcc ggaatagtcg accattcgct caattaatga
     1561 ctctgctcct tcagtggtgt taggaaatga aaaacctttt agtaatacct ttccttcctc
     1621 gttcatgata gaagcaacgt gagtgcgttt accgatatca atgcctaaaa agtacatgta
     1681 tatctgacct ttcaaaaaaa gaattttgga ttgaaatcca ctcaactgaa aagcgtcact
     1741 accttgtacg agatacggag taaagtaagg actttcaaca tctaactcat acgcaatcca
     1801 cttatcagag agaggagcca gtctctcgag tgcgggttca atgacccaag gggggaacgg
     1861 cttagcctct caatccaata aatatattat ctcaaaagaa atgagttaat accaaattaa
     1921 aataacgatg gttcataggg ttatccaatc aaaaccggaa ggttaaaacc ctaactctaa
     1981 tatacaagga ggaatcctcc tttattaaat atctgttaat atatctgtta aatatctgtt
     2041 aatatatctg ttaggggggt cttaacccct tgggagagta agtattgaag gggtatgaag
     2101 gtaacacttt tggacagtaa aggtaacaaa agtggacagt aagggtaaca tttttggaca
     2161 gtaaaggtaa caaaagtgga catttgaggt atcaaatcag gataagacgt taccttcggt
     2221 tttcttgatt tgaaaatcga aaaagtagaa aactagatta gtaacaaatc tttgttgact
     2281 gttaccttta gttgtgattt aatttaggta acaagcagaa aaaaagcgtt tcctttaatt
     2341 tagtaccaaa ttaacatgat taaagggatg caacaagaag attggagagt aatcaatgtc
     2401 tcaagaagtt accgaaaacg ttattgataa tgatgtagat ttagtatcag atcaaacaaa
     2461 tgcaattgtg cgttataaca atggtttgaa cacaacagct ttgcgaggat ttaaacctgt
     2521 cgaaatggat gtgttttggg ctattttatc aaaaatgaaa cgtcaaggaa cagcagaagt
     2581 aacttttgat ttcaacgtct ttcgttattt ggtcaaatac gacagaagag aaaaggacag
     2641 tttttattct gtgttaaaag ctttaagtga caaactaggt actttaacct accgctttga
     2701 agattcagat gtctatgaac agctgtggct ttttcaaaaa ttccgaatag ataaacagca
     2761 agaaacagtt acaatcaagg caaatgagga gtttgaattt atcttaaatt caattggcaa
     2821 aaactttact cgttttgaac ttgataactt tacaaaacta agaagtagct acacaaaaga
     2881 gctgtatcgt catctcatgc aatatagaaa taaagagtgg cgaaatggct attgggtagt
     2941 caaagttgaa gattttagaa gagcgttaag tattccttca agttacagaa tgacaaatat
     3001 tgacaaaaga atattagagc aggcgaaaga agaattttta gctcctgatg aaaatggtat
     3061 acaggtattt gaatcttttg attatgaaaa aatctatgcg aagaaaggaa gaaaagttga
     3121 tcggctcaga ttcactttta gtgaaccaga atcaaacttg ccatccattt caatgcataa
     3181 ttggttagaa gaagactgaa aagaagcagg ttgtaatttt aatacaacct gcttcttttg
     3241 aaaaaagtat gggtaacact tttggacaat aaatgcaaga agtttttttg tgaccaaact
     3301 ttatattaga ttcctgtgat tttgtgtgtt agaaaagccc tgatagaaaa gcaaattcaa
     3361 acacttttta atatgtatgg gtaacacttt tggacatgtc aataataaat atattttctt
     3421 tcgtcttttg cctaactaaa ggtaacactt ttggacagta tagttttgta aactaattag
     3481 atcgggttta atgagatata tcttagcatt tatgtaagaa tgaaggtaac attaatggac
     3541 atgtcaactg tttgaacaaa aaaatataga ctatttggat ctgatttttc atttttccaa
     3601 acgttaaaaa atagttagtt attttattaa tagatagtca tccttaatgt ttgaaatagc
     3661 agaaaataag atattgtttg ataaatgttg ttgttataag gaaaacttga atttgttaat
     3721 tgatttattt atgttattat gatactataa taacataaat gtgttacgcc cgaacctcat
     3781 tttacaaaaa tgaaggggtg agacaatgga tgctattaat tggcatgaat ttgttaacta
     3841 tatggagaaa ctactagatg atctgaaaag ttttagcatt gctgatgccg taaaacatgg
     3901 gttgccagtt agtacaacga tgaaaatgcg agggttgaag gttccggatg aaattatcaa
     3961 gctgaggact aaaacaatca ttgaaactgc aaaaattgtt gacagtatga accttgattt
     4021 gaaaggcaaa aaaacaactt atgagaaaga ctttgtacgt tttttaaaaa atgttatcgg
     4081 aaaatatcta gggaaaaagt cttttactga attgagagac gaagagattg taccaactcc
     4141 aatggttagc aaaattcgca aaaacaaagc cgagggaaat tactatcttg gcacaatagc
     4201 aaaaatcgtt gagtatttgg aaaataaaga gcaaaagaaa ggaagatagt tcgtgtgata
     4261 aatatgaata gggaagaatg taccgaaatg ataaaaaaac ccgaacctag aattaccgaa
     4321 aaactgactg attgcttgct cttttacgaa agggggcagg caaactaatg gaaaaagatg
     4381 tcaattcagc atccactaaa gatcaataca atatcattgg aggattactt ggtgataagg
     4441 ataagagaac acgtcatgga ttacgcatta tgcttgcgac atttctaggg gtgtttcttt
     4501 tattaacagg cttgggtagc tacatttatc aattaggaaa ctataattat tatcttgtca
     4561 acaatacaac accaatcggc tcatcattga cgtttgctcg atcaggagca gagatcacta
     4621 tctcagatgt ttggacagat aaaactagag atgtaacggt ggtcaaactt ggttactcag
     4681 gaaacgcaag aaaattgcta tctacacaag gtaaaaatta taacttgtat atggcaacaa
     4741 aagaaaaaga tcgtcctaag aagcttgaaa tgagttatgg gatgttaagt accgatggtg
     4801 atgctttctt gttcctaaaa ggtaaattag atgaaagagc ttatcaagtt tttattgcta
     4861 accaagtatc actaatttca ggtgattata cagctgatga tgataccaaa gtcaatgtga
     4921 atgaatcaga ttcagtcacc aaggcacttt ctcagtattc tatgaatgat gttgataatg
     4981 aaggagtaat ttcttttggg aaaaaagcaa aaccagttaa tactcctgat aatatcaatt
     5041 tcagaataaa tccatactca gatacaacta acgtatatca aggaagcttc cttaccaaag
     5101 atggcgatat tgattactca aaagttgttg ctgtgacaag taagaataat gttgtaaata
     5161 agctagaaga ccagttaaat gcttcaaata ctaacatcaa gaaaatcgaa gccacaaaag
     5221 ctgagtatct tgaacgttta caacaaaatc ctgacgataa agattcgcaa gaaagtattc
     5281 gtcaactaga cagtcgaatg gagaccgaaa ttaaaacaat caatgataca caaaaattga
     5341 ttgatcagta caaaaatgct gactttaaca aagattcttt tggagatatg aagacaacat
     5401 ttaagtacct aaccctgact gactagaaac aaattattct tattttagag gaagtgaaca
     5461 caaacagatg gaaaatagta ccgagttctt taaaaatatt caaaccgaat tcaatcatgc
     5521 tctcacacta atgaaaccac cgaaacataa gttagtgggg cgtgatgacg aactccatga
     5581 gttgtcactg atcttaaata gaccggaaac accacaagct atcttgttag gtgaagcagg
     5641 aacaggtaaa acagcactgg caagtgaatg ggctaaaaga cagcgacaag aggggaagaa
     5701 agtacaagta tataaattat caatcggaca attgcgagtt ggaggagatg acgtcctcca
     5761 aacacgtttg gaaactataa ttccaagatt gaaggaatac gaacgaattt taaaatccga
     5821 ggacaaagat aatgaatttg tcctctttat agatgaattt cataaagttg tcagttcatt
     5881 tgggagcggt aataaaattg ggggagattc gctgaaggaa cctttagcaa atgaatcact
     5941 tcagttgata accgctacaa ctagaaaaga atatgaaaac tatatcaaac gagatgatgc
     6001 tttaggtaga cgactacaac cgttagaact caacgaatta aaaagtgatg tcgttgtcga
     6061 tatactaaga caatggttga atacatcatc tgtcaaagat gatcctgaag cttccttgaa
     6121 attatccaaa aagattcctg atgaattgct tgaagaaatc gtacaagcaa acaagcttta
     6181 tcgtgaggat ttatccgaac cggctaaatc aattgccgta atggaagatt tgattgcgaa
     6241 ggatcgtgta gatggcaatg cgtataatcg agaaagcctg ttacaaatat ttggaaaata
     6301 taatattcaa ctagattctg attttgatat agcaaaattg gaagaggagc taaagtccat
     6361 taaagggcaa ccagtagcgg tatatacggt cacgatgttg tttaaacgaa tgaaacttgg
     6421 atttgatcgt tttaaagggc gaaagcaacc aaaagcaacg gctttgttta caggatcaac
     6481 aggggtcggt aaaactgcga tggctaaagc tgtagcaaga gggctatacg gtgatgaaaa
     6541 taaagtcctt ttagtaaata tgccagacta taaaactaaa gatgatgcta tgcggtttag
     6601 aaagcttatc ggaacacatg tccggcatga tccatcagca atcattgttt gtgatgaata
     6661 tgaaaaagca ggcgtagaaa cacttgatgc ctttttatct attctagatg aaggaagaat
     6721 cagttttact gaaagaggat gggatgatcg agaagttaat gataccgtct cactttcaaa
     6781 tacgatttta tttgcgacaa cgaacgcagg ggctgagatg tacgattcga tgaaccgata
     6841 cagctcacta tctcgtgttg ctaatcagga aaattcaaat gagtacagca ctgagcttaa
     6901 aagagagtat cgaaaggtag cattagagat taaacaagct ctaattggtg aaggtctaaa
     6961 acccgaagtc atcaaccgat ttggacggat tgttccattt tgggctttat cggaacaaac
     7021 tcttttggaa attgcacaca atgaaatatt gaaaagtgct gaattggtaa gaaaacactt
     7081 aaacgtagac ttacgccttc caccacagaa aaaacatttc tatggtcaag aattttatgc
     7141 agatgatctc tccatgcact tagtttttga tagagcttca caagaagatt ctaaatcagg
     7201 gggtgcaaga aatatcttgc atctggtcga atcagaattt gaagaagcaa ttactgaagc
     7261 aataattacc tatcctaacg ccaaaatatt tgacgttaaa ttgagcaaag caggagggtt
     7321 tgaagggaaa ggcgatacgg atgcagaaat tatcgctaaa ccttccatcg ctgaaagtaa
     7381 agaaaacaag tttgtgtttt cggttggagg ggtgtagcta tgaaatttaa aaagtcttta
     7441 cttcaaaatc gtaaaattaa gcgatgggtt gtaagaggtt tagttatcgt gggggcactt
     7501 gcagttactt taggcgctcc atttggaatg aatgatccaa cgtctgatga tcaaggaaat
     7561 aaacaagaaa cagttgcaaa tacgaatgtg gcttatgctg aaagtaaacc ggcagaaaaa
     7621 ggggtatgga cactatatgc tcgtatcctg atgggagata aagaggtcaa tgatagtgcc
     7681 actaaaactg gtcaaatgaa tttacttggt caaggaggta ttagtgctga ctttccttat
     7741 tctgatattt ccaaacaagg taatgcaatt gccggtggta acgcaggtac gcaattagca
     7801 tcaaccttag cgacttataa ttactatggg tatatcgaaa cagtttcagg aaatgcgatt
     7861 gcaagtcagg catcctcttt tattggtggt atcggtagaa cgcttggcag ttttatcgga
     7921 tcaattagct tgatactttc taatattgct gaatccatca atgaactcct tggaaaattt
     7981 ttcatgagta tgaatatatt ttcacttatg ggttggggta aaggtggatc ggatgtttct
     8041 aatccggtag ctaaggcgtt aactagctta cttaataatt tgggattaaa caatcaatta
     8101 tttaaagcga tagcaagctt atctatgctc ttttttacag caatactagg ctatcaagga
     8161 atgaaactgt tgtcgaaaga gggtgttaac tcaaagggat tatcaccagt acttgaaaaa
     8221 tggattgttc gtatttttgt attgtttgtt tttctaccag ttgcaggatc gttattggga
     8281 gtttatatca atggtattca gtcagcaaaa gaagatggaa aattaccaac aaatgttgtt
     8341 ggtgattaca tcattaatac aagactttgg gcttctactc aaaatcttgc tccatcatcc
     8401 gatactggtg ggaatgtgcc aaacggaaaa ccaacatctg aaattaaatt tgttgacact
     8461 gattatgaac catctaaaaa atcagaaatg atcagaatga tcaatactag cagttacgct
     8521 agacaaaata atgatctaga ttccaattca gtcggcatgg cattgttaaa agaatggtca
     8581 agtaatgata cgtttaatgt caatacttat gccggtgatg tgaatgtagc tgaaaatgat
     8641 gcttatatgg gaagagcgcc tgctgtttgg acaggctata atttagggga tggaaaagaa
     8701 ggaaatgcaa ttaagaaacc taccaattta aatggttata tttggtcagc taatcaaaac
     8761 gttacagacg acaatcgcaa gcctgatgga aaaggaacaa cgaataactt tgatgctact
     8821 caaccttggg gtgttgctaa caatgcaacg tttagcacac agtcggtagc gttgatgctc
     8881 caatcagcac tggatacatc aggggctaag ttttatgcgt ataatatcgc tccatcagga
     8941 attcaaggta gtgctaagaa tatgtcaaca gtaaagacag aatggcgtac agcttcttta
     9001 gttggtcatg gagctatggg taaattcggc tcatggttag cgatgaacgc tgaatcaatc
     9061 gtattagcag tgctttatct agcttgtgct tgggcgatgt ttaacgttaa tattgtaaaa
     9121 gccttcaaac gtgagtttgt gcgagtttgg gaagtagtct taacagggtc tcttgccgca
     9181 ggagcagggg ttatggcaat ctcattagga gtcgtaacca catctcttat cggattacaa
     9241 ttacctattt ttctgatcac ctttgttcgt gcatttggag atacggctgt aggattcatc
     9301 cctaacacat tacttggtga tggtgtagca aatattgtac gatccgctat ggtcttaggc
     9361 gtaggatacc ttctagtctt taagaaaggt gaaaaggtta ataataattt gattaatatg
     9421 cttattggat taccacttga agtagcgttt ggttttgacg aacgtgctaa gttattattt
     9481 ggcaatagaa atgatgctgt tcgagttgtg gatggcgttt ctaaagcaat caaaaaaagc
     9541 caaggggcta aaaatcctta ttatgatggt ggcagaggat cagtaaaaga tatggtcgca
     9601 ggaggacttg gagcagtcgg agcgcaagga attgctgatc gtttgaacaa tatgggagac
     9661 agcaatgctg atgctaagac aatcgctgat aaagttgttc ctgaaacgcc tgcttcagta
     9721 ccatttaatg gtggcggagt tagtggaaca atcaataata cctctttcaa agatttagtt
     9781 cgtcaagatc aattacgtaa taactcgcaa caatatagcg atcatctagg acggatggtt
     9841 gatgaaaaag gaaactttgt cgatgaaaat ggtgaagtat taaaagacag taaatttggt
     9901 aaagatgcca aaggaaactt tattgatgac aaagggcgtt tggtcgatga agatggaaat
     9961 ttgatagata caaacggtaa accattggaa gacaatgaag atggaaacat cggtatggat
    10021 agccaaggac gtttaattga tgacaaaggg cgcttggtcg atgacgaagg aaaccttgtt
    10081 gatgaaaacg gacgtaaatt acttaacggt gattttgggc aggatgtcaa tggtcgtcta
    10141 gttgatgata aaggacgatt agttgatgaa gacggaaatc tggtcgatga gtttggtaag
    10201 aaacttggaa aacacagtaa cggttataac gtaaatggcg atacagtcga tgaagatgga
    10261 aacatcattg attcaagaga tgctcttgta gcagatcgaa ataaagaact cgaacaacaa
    10321 cagcatacac caattgcggt tgcaatccct gatgatgaaa gcgatgaagt tattttagat
    10381 caagcacatc aagggttaag agaagaagca aaggataaag ttgtttcaga tcaagcacaa
    10441 gaagagctaa aaaataaatc gaatggtgaa gatgtttcaa accaatatca tcaaacggct
    10501 ggaaatgtac ctgttgaagg tcagtctaat gagcttcaca acaactttga taatgttgct
    10561 gatcggaatg atacagtctt aaaagaaaaa gaatcattaa gacaagaaac agataaagac
    10621 caatcaaaat tgaagaaaac aactattcct actcctattg gttcaacagt tccgaaggtc
    10681 aatcaagcta acgattctga aacaaacact gggttaggaa tggcgaaaga caatcaatcc
    10741 attagagaac ctaaaaagac gactgaaagt agcaagtcga acaagtctaa atctacttca
    10801 aataagacag ggaaatctaa ggaaccatct aaagctaaat cagcagtaga atatctatct
    10861 aaaactggta agaacgtgta tacttcagca gataaagctt tagcaaaagc gccaaaagga
    10921 agcttagcag gaatgaccta tgaagctaag aacgaagcta tcaaattagg taaaggaact
    10981 gctaaagttg ccaaagtaac tgctaaagga gcaactaaaa ctggtaaagc agtgaagaaa
    11041 tctacgatgt cggtgaaaga ttatattgca aaatatagcg aacaaaaacc aaccgataaa
    11101 aagtcttaag aagaataaaa agagggagca ttaatatgct ccttcttttt tgttcttctc
    11161 ttcaagggga atgtcaagaa aaacttaatt gtcttcaaaa atttcttttt tatttggtag
    11221 taaatctatg aaatatttgc ttttttagtt atcggagaat aagagggctt atgctagtta
    11281 gttaaaatca ggtaactagt cattaaatgc tgataaatca gcaattttta cgaaaaaaaa
    11341 aagaaaaaaa tttgagggaa aatacctttc aatctacaaa aatttatgtt attatgataa
    11401 cataaagatg taaatatcga agcatctttt ctgtattccg atcattgaca agtgaacaga
    11461 gaaaagtttg cgatgatata attatcttgt gaaaaataat cgaaaggaag gatttataat
    11521 atgtttgatt ctattaagaa tttggtttcc ggaatgacgc cagaaatgaa aacgttggtt
    11581 caagctattc tatatttaat ggctacattt tttgcattga aaccaggatt cactgctatg
    11641 aaacatttcg gtgatgcaaa atggggagaa ggcttaaaat atgttggagc tgttgttgct
    11701 atttttatta tccctgtaat tacaactgtt atgttgttaa cgatgggtac taaaacaggt
    11761 aatgacttaa accaaaaagc taatatgatc tcagctctta ttcctgtggt tggtatttat
    11821 ctagttacaa agtttccaaa agaagaacaa ttaggttaaa aggtaaattg agcaaatttg
    11881 gatgatatgc cgatttgttc aatttacttt ttgctaaaaa actacactcg aagcgtagtt
    11941 tttgaagtga gggaagacaa tagatgtaag atggacagag aaaagatgtt ggttcacgca
    12001 aacgaagtat tgacgagcgg ttttaaaggt accgatttgg cgaagataat gaatatgaac
    12061 gtaaaccagt tctatgatta tcgaaacggc aataaaaaga ttgaaaaagc cagacttgaa
    12121 acgctcatca aatttgaaaa agcgtatcta tacgtgttag acaaacagaa aagaactatt
    12181 gaccgaaaaa aaggagtttt acaataatgg caaaattcaa agatattacc gaatctatta
    12241 attatgtagc agaaacagtt cccgtagttg tactctcttt gaagccacaa ttactcgaag
    12301 tgtaccaaaa agaagaaaaa gaagcgaagg caaaaaaagc taaagcaaag gtatggaaag
    12361 aattcgatgc gttgctgacc aaagatttac caaagaagaa ttacgtggtg attgaggacg
    12421 tacaaaagct cagcagtgag gaatttccgg atgaagaaga tgcgattcta gattccgaaa
    12481 agtacgttgt tttattaaaa tcaagtctga attatgttgt tgatttaccg gaaaaattca
    12541 aagaaaagct attagctggt gtccgcttta tggatagtaa tggtcaatta ttagttattc
    12601 ctgatgatcc taatatgttt gaaagagaag ttgaagaagc tacagacttc tacagtgcag
    12661 tagatcaatt agaggtagct actaaaggct tagaaagaat tgctgagacc ttcaactctt
    12721 ctgatgcggt tgaatttgaa gctgtagcag aagccgaaac agaagccgta ccagatgaag
    12781 aagaagctct ttttgaggat aatgacgaag caactgaagc aggaaaccaa gaagcaagca
    12841 tagaagaaac acaaacagaa acctaccaac aaccaaaaat tgatgaaaaa cccgaatccc
    12901 agagtgataa agaaaattat gtttatgaag ctgaaaaaat cgtatctgaa agtgatgtac
    12961 cgaaaaattt agatgctgat ttgttagaaa aagattttga acagatcata gccaataaat
    13021 ttcaacctgt agttttagat gaggttagtg atcctgttga tttgaacgaa gagatgcgat
    13081 ccattgcaga gttaaaacca ttgctgtttt tagctaaaga gagcatgaac gctcgtatct
    13141 ctattgctaa taataaaatc aacagtgaat taaataaaac gaaagagcaa ttgctactca
    13201 aaggaaagca aatgttagcg gatgagcttt tgcggataga ggaagcaaca tcactaagtg
    13261 gtcaaaatca gtttacaaat acgctatcgg tcgctaaaga aagctacgaa gcagaagcat
    13321 ctaaaattga tgatttagtg atggaaaaat ccagtatcat tcgtaaagat tatgatatgg
    13381 cgaaacaggc ttatttggaa gcagaactaa agaaactaga gcaacggtac gatgctgaac
    13441 atctgccaga agttaaacaa caagaagatg aatttagaaa agcaactgaa gaaagtcttg
    13501 atcaacaata ccaagaagca attgaatcta ttaaaaacga tgctttgaaa caaaatcaag
    13561 aagcaaaaga agttgtggtg gaacggatca tcaattcatt gaaaccggaa attgaaaaat
    13621 ctttaacaga agtgcaagaa aaaacgttag agattgtcga aaaaacaaag gcagaaaatg
    13681 aagctgagtt ccacgataca aaagagaaaa caacagatgt ggttgtcaaa tattttgcga
    13741 acaaagaaga agttgaaaag ctagtagcta aagatacgaa tgagttagaa tccaaacttg
    13801 ttgcacagca aaacattatt gaagattata aaaatcgagt aaaagagttc aaggatttag
    13861 cagatggcta taaattaaat agccaaaaat tgactgaaca aagtgaagct ttacgagaag
    13921 atttatctcg tgcaagaaac gacctatcac aaatggcgga aaaaacaaag tcactagtag
    13981 ttactacaga aaatcaaact gagtctaaac gtgattttac aagcaaaaag atggcgacta
    14041 tcatcgctgt atcagcaatg gcttgtggaa cgattttggg ctcatcctac ttcattagtc
    14101 ataatcacca agaagcccaa gctcaaacag aaacctcaat ttcaagagtg gaaaattcac
    14161 taggatcaaa tacagcttct acttcaacaa cagacaacaa ctatgaagca acaccaacga
    14221 cagaatttaa agtcggagaa gatgttgtca tcagtattga tgggaaaact actaaggctg
    14281 aagtactttc cattgacggt gataaggcag ttgtgaaagc tccagatggg aatcaataca
    14341 aaatacaagc tcacttaact gaaaagaatt aaggagggct aaaatgcgag aagaaaatat
    14401 tgtttatcca gtaattgaaa atcaagcaaa tacaaaatct tctcaatttc ttcaagaagt
    14461 gcaagtcgtt tatccggtta atgaactacc acaaacgaat gaaacagtga gcaagtcaat
    14521 atcattaatt ggcttgctta ttgttgcaac agcgctaact gttggtgttt atcgtaagct
    14581 gaaagtgaaa ggaagtattt ctgatggcag ataactcaaa ctatggattt aaacataatt
    14641 tgcatcaaaa accttctgaa acaatagtca gagttgattg gcttgaaaaa catgtcaaca
    14701 gcacctattc tgttacagca aaaagactaa ttgtttcagc tattgttgtt gcagttgttg
    14761 ttcttggttc aatcgtttat ttggtttccg atacgattaa attgagattt atttttattc
    14821 caacgctatt gatgataatt ggttttctta tactgattag tcagtatatg tttattgatc
    14881 catctagcgg atcactgatc tcaggctcta ttagactgtt taaatcagta gttaacacag
    14941 caagagtaac gatggggaaa aagaaaccat ttcgaggaac ggagattttg aaagttagac
    15001 aagatggaac gattatgtat cgagatggta gtgtagcact aatgtttaat ttggatggtg
    15061 ctacctcgtt aaccgccttc ccagaagaaa tcatagcgca agaaggcaaa tcaattcaat
    15121 accaaaatgc aagggatcgt tccttggctg aaatcaaaat tacttcatca caacctcaga
    15181 atacggaacg tcaaatacgg aacatgaaag tacaaaaaag aaatgcaaag agcaacaaag
    15241 ctgtgtttga catagtagat caacaagaga ggtttgtatc tgaatatatt gatggggtca
    15301 aaactacggt gatccaatat cttattttga agtcacaatc tcagatggta ttggatgaag
    15361 gcgtagaacg tttagaaaat tacacgagta gagggcttta ctatagcgtt actcagttgt
    15421 cagctaaaca aaccaaagaa atattatcag aaatttatgc actgaaataa tagaggtgag
    15481 aaatttaatg gaactaatga gtagatcgaa gctacttaat ttcagaaaaa agaaacacaa
    15541 aaaagagcaa aatgctgata gttctaaagc ggtaaataat acttatgtca gtgatttaac
    15601 atcattggtt ggttatcaat ttacaccatc ttacattagg acaggtaatc ggtggggaac
    15661 gattgttaaa ttagtgaata cgtatggaat gaacagaaat tatgaatttg gatggatggt
    15721 taaagctatt cctgaagtaa aggttgaagg agtaaaagct tacttaatcg aagcggataa
    15781 gccagtttcg caaaaagatc agcaagaaat catgcacgaa aatgtcggtg gatcaacgaa
    15841 cagttatgaa aataacttga acgataaaaa ttcagatgca gaaatcaata aaactgttat
    15901 gcgaatcatt gatttgaata cagcatctct gcttgacagt acggcaaaca aaattattga
    15961 ttggaagtta gaaatcctct tagtatccga ttctcctaac aagatcagcg aacagttgat
    16021 caaactgcaa cgcctgtatg atgaaaaaat gtatggatta aaacttatgt cttttggtgg
    16081 cgatcaacgt gatctgtttg ataggctttt aagtgagcca gtcggtagtg tttacgaata
    16141 cacatctatg agttctgatt ttgcaggaaa tgatcatgct attcgtaaag gcttagatga
    16201 tgcagatgga tgggcgatcg gtacgatgtc tacaagctac gcttcaggtg tagcgtttat
    16261 ggcaatggat cggtctttta agggtaaagt cttagtagca tcccacaaag atgcaacaat
    16321 caaaggctat agggaagaaa tctcagcatc atcactttgg gggcaattga ttgctaataa
    16381 cgcaatggca aatcataaac ggacgtttca catcgtatta aataaatttg actactttgg
    16441 tgatccgaca ggggaaacat ttgttgctaa gccatcttta aatagtgtga tagagaagat
    16501 taatctagct gaagaaggac ttaattcttt agagcttttt ggtgatcgaa aggatattgg
    16561 agatttatat agacgtaatc ttgataaact aactcagatt ttctatctgt tagcggacag
    16621 agaattaaat cctgcaacaa gagtgttgct gaagaaagag ttggggaact tttatattag
    16681 tcagaagctt tgggatatta actatgaaga gcggtcgaaa ctgttagtaa acagcagtga
    16741 tacaatgccg gttcttggag attttatcag ctatttaaca aatgctgaga caaaagcgaa
    16801 aaaaaccggt acagaaaaag aaattgatcg gattattgta ttaaaagata cattgcgaac
    16861 agcattggat agcttctcta agatgttcaa tcagagaact acattgaaag aaattggcaa
    16921 gagcgacaaa ttgcagtttt actacaattt gagcgattta agaacagaaa tcaatgtcat
    16981 ggaagcccaa tttttaaata cctttgactt catcacgtca caagctaaaa aaggcgatat
    17041 cgttatgatc catgggtttg atagattatc agttgaaacc gttggattaa ttaaaaagcg
    17101 cctaacaatg cttgaagaag atggagttcg gcttgcgttt ctctttgata ctatcggatc
    17161 aggagaagat aaagacggta tcgaaacagc tgatattttc aatacagatg gaaaaattta
    17221 tcaaagtttt gaaagtgatt ttaactacac tatcatcggg aaaatgacca gtgaagaatt
    17281 gatgaagtat gaaaagaagg ttgctcctgc aagaaaagga gaagatgatc aaaaactatc
    17341 agtggatttg agacagtatt tgacatcagg tcgaagcaat aaatttcaaa tcagaagacc
    17401 agtcgatttg acgaacaatt ttgttattgg agagtttatt atttaagttc tatccttatg
    17461 gtatcggaaa attgtgtcta gaaggaagaa atatccgtca aaacaaacca tacttttttt
    17521 ggaaaagcgg agttttagaa caatttccta ggagaaatag ataaaacaaa aggggcgaat
    17581 ggttatgaaa caatatttaa atgaatggaa agtgatagag gggtcattag tagcacaaag
    17641 aataaaggga ttacctgatt gcttagagaa ggatcatttg tttcaaatac gagaaatgtt
    17701 aaaaaaagaa cagtttgatc cagaccaatt tttagtagtc gagtatccaa ctattggggt
    17761 gtactgttgt aaccatatca atgatgaaaa atattttatt attcaggaat atgaaggaca
    17821 attaactcct ttctatacta catgggaaat gagtgaggat gggatcaata attttccatg
    17881 tgaaagcatt gaagagtcaa tatctcaaac cgaatgctaa ttgattataa ctataagaaa
    17941 ggtagtgagt taatggatac agctatattg tatattaaca taattccgtc tatatccttt
    18001 atcattactc ttacaatttt catacgtaga aaaaaatttt ttacagagaa gaagcttgta
    18061 tgctttttga tgaaaagtgg agtagtattt cttttttctt ggttattgct tattttatca
    18121 acttcaagtt ttttaattat attatttgcg actgtgggtc ttatagactt tggtgtaata
    18181 tcaggagcag taatgtggtt ttggttaact agaaaaccta aaaaaaggaa caagaaaaat
    18241 ggtttggaaa ataaacgatt atttagatta atgacaaatc attaaagcta taggcatatt
    18301 aagagataat atttaccaat ttcctgattg ttttgaaaac gagcatctgt atcaaatcaa
    18361 aaacatgatg aattgatttt gttatattga aatagatatt aagaaaaagc attccttgtt
    18421 aaaacacaag ggatgctttt tgtcttcact taatctgttt aatataatct aacaactttt
    18481 cttcggaatt aattttttca cctaggtatt gttcagactt attaacaaga ttcaatttaa
    18541 gatcatttaa tatttctgga tacgtatcac ttttcgattc agctaattca tacagttttt
    18601 ttagttcctt atcttttaac tgaaacttct gatccatagt gacagcattt ggatatagaa
    18661 gtccattata atgtgtgcgt aaatccatgt ggaatttttg tttataaaag tctagcagat
    18721 tagaaataaa agaagaaaaa taaccattat agtaaaactg attatttcta tttaagttat
    18781 acaacaattt attaaattga tcctgattta atttgtcgta aatccatggc aatatttcca
    18841 tgtccttatc tgagtatcta aatgcaggag aatcggtcag atgctcaatc ataaaatcta
    18901 agaacatctc tttttcacca aaatatagat attgattata tatcgtttct aaggtagaag
    18961 ttgctagaga agagactctc atgttttgct gaagtattgg atcaagtagt ctatttaaat
    19021 gttcttgtaa ttcttccttg gtgtttccta gaactgcaag tgaacgtaga cggtagtaat
    19081 cttctatatg tgttgttatt ggctgttttg gttcaaaatc ctttattgat atttttaagt
    19141 tttgaattat ttcttctgaa aaatcacgct gtaattctgg atgactagct ataaaggtaa
    19201 gcaaaaagaa gccttttgta taagtaatct gttgattttc tccaagtgat ttattaattt
    19261 cctctctcga ttcagctgtg aacctgtagt ttgagatttc tttttttaga atttttaaat
    19321 aaaattcttt gttatcaaaa atcagggcat ctagtagttt gaaagtactt actctatctc
    19381 tatctacttc ttcattccca taaggtaatt taaaaacaaa cttaaaggct gttttacaaa
    19441 ggttttcttt ggctttctca cttagttttt tcagatagct attttttagt gcagagtgga
    19501 attcttcagg tgtaacagac atagaatgta ttcgacttgc atattgtatg atttcgttcg
    19561 ttactttttt tcctaatatt ggatttttta gaaatacaat ttcataggat ttccgtatca
    19621 catcctgaca agtttccttt gttatttctc ttaattccca agtgtcacca ttgtaagtga
    19681 ctactggatg agctgaataa tttctttgat ccttaatata ttttaaatcc tcatattcgt
    19741 agctatcaat tagctcagtc gaattataag ttttttctag tagctccatt tcccatcttg
    19801 gggagtttgg atcacttttt cttaatttag ctatttcttc taaaattttc ttagaagtat
    19861 catcctcgta tatatttgct aaatcagata atttatcaat taagtctaaa acaagcgcac
    19921 tgtaattgac agctatcgct gaacgataat ttccctgata aaaactactt ctgacttctt
    19981 ctaataaagg aacaattcta ggatttttaa ctttgtttat ttcatcatct aatataaaca
    20041 tataactctc cttttttgtt gatatgacaa gtttaccaca aaaaataaat taaaaaacga
    20101 gacatattta tgttattatg gtatcataat aacataaata tatggaatga gatgatgcaa
    20161 aatgggcttg aaacaatcag tttacatctg gttatatcct gataatccca tgtcaataat
    20221 ggctgatatt tttgtaggta gcattttatt gtgtttactg tatctaattt tcttccacaa
    20281 tttgatcggg tacatgatcc ttgattggaa aggaatgttc gatatagaaa gaaaaccatt
    20341 taataataag caaatttata ccacctttgc aggtggagat ttggaagaaa aagaagttga
    20401 tgtgacacat gaaattggat tagctttatc aggaattcaa gatgctgatg tattaggatc
    20461 aatgataacg gataccggat ataacgccta tgaagtattt tatctcatgt attttatatg
    20521 gcgagatagt caagatatta tcaacggtcg tgctgaaaaa acaatcattt caaatactga
    20581 aatttctttc tgtatgggtg aaggggaaat gattgtatat aaagatggat cagcaaacat
    20641 taataccgcc tttggtgata ttgaaactag tattaaagag cttagggagt atgtacagaa
    20701 tccctacaat tttcaatatt tagcaggaag aataaattta gtttaggagt aagaatatga
    20761 aaaaaattat tgatcagcca acaaatcgct taatagtcga aggcgttatt ttcgtaacaa
    20821 tgtatttcac tgtgggaaat ggtatttcaa atacaatcat tcaaaaagac ccacaatact
    20881 tgttgtttag tctttatgct tggattgtgc agatgctttg ggtgtcttta ttaatgctgt
    20941 tgttttattg gatagtacgg gttattcttt tagtgttaaa caaagcaaaa aagaaaaagg
    21001 ataggtgagt aaaaatgaaa gcaacaaaat taaaacttgt actattttca acagcagtat
    21061 tactaacttt atcagcatgt ggtacatcaa agagcgctga taataccaaa gacacaaaat
    21121 ctagcagtac agtggtgact gaaagcagtt cagcgcagac atctacaact atgtcttcaa
    21181 tgactgagaa caatgctgaa aaatttcaaa agtataagga cgtaaatcaa ggtaaaaagc
    21241 cggaagaagt ttataacgag tttatctctt cagtaaaaaa tgatgaaagt tatgggaatg
    21301 atccattaca aaataaaaat ctgactattg ttctttacaa acctgattgt cctgattgcc
    21361 aaaaagcaga atccaaaatt atgcaagaaa tacaagaagg atttagttat gctccaacat
    21421 taatgcacga tattaccatc ggcaaacctg cttacgaata taatgcagtt gcggtagata
    21481 taacagatgg cgtacctgaa tgggtgcaaa atatccctgg tctggctgat gagaacaacc
    21541 aatatcacac gccaacatta gcagtagtaa caccaaagaa aattgatgga acactatact
    21601 gggatttaat gggaatgaca acaaatacat ccgatgaaat gattgaaagc accagtaact
    21661 actatatgga tttgggagcc tataaatcat acgaacagtc tgaacctgtt atggatgatc
    21721 aatctactat taagtagttt ttagggcgta agccctattt ttttaaaatt atgcgataac
    21781 aaattaatga tttctgaaac aaacgttctt actcaactac tcgaaacaca caagctaatt
    21841 aactagatta taaaggagaa tgcgtaatga aagaaatcag tgcaagtgaa gcccatattt
    21901 ctcaaaagaa tcaggttggt atatataagg tgcttcatgg tgaagggatt tataaaatac
    21961 agttacttac tggttacaat aacggtgcaa atgggaagtg gttaaaggca acaagtattc
    22021 gtgaacttag aagtgaagta gaaaaaatta ggcttggtct cacaagtgaa gaaatagcta
    22081 aactgaaggg aaataattct tttgtaattg aaaaagaaaa ggaaaaacat ctactttatc
    22141 gtgagctgaa aattgaagaa tactatgaaa ttgatttacc tgaaaaatta agaattattg
    22201 atcaacgttc agaagaaaaa tggaaaactg aaatatatcc aagcttaaat gactacaaga
    22261 catcaggaaa gcccatgtat aactacacag gctttttaac ctacacacaa tacaaatatg
    22321 cctttcgcaa attaaaaggc attaaacaag ctgaaattgc cctgtatgag atgaaaaaag
    22381 tggatagatt gaaattgatt caagagttag tgcaaaatac atggaatcaa gcgattgtcg
    22441 agctagaaaa agaaaatggg aaaattttaa ctaataaata tctgatcgta aatgaaattg
    22501 aagaattaat tggtttattt tcatgtgaaa aaggtgaagt cttcttgaag tcatcaccaa
    22561 cctattcaat tttggacagt ccgtatcagt ttatacaggc aaggagatga gttagatgtc
    22621 tagcttgaaa gatgactata tccaagcaaa acaagagtat gaaaaaatac agatgttgaa
    22681 atctgagaaa gtcgaattat ttttaacaca aaaattagca gaggatcata ttgctaactt
    22741 agatgctaaa actttagagg aatatgatcg gcagtttaat cagatacatc acaatatcat
    22801 tttgcttgtt gagcaggcaa agcaaaggtt tgaaaaagcg gaatatgatc tattagatca
    22861 aggaattgta tgcttggata agggattgcg aagatattat ggtgcagagt taaaaaagca
    22921 aaatgtagat agaaatgcac taaaacatga aatcttagaa ttgataatgc aacgaatttg
    22981 aagggataga aagcaaatgt cacaaaacat tagtgctgta aaacaattaa tagaagaaga
    23041 tttagagacc acagttcaga gattagcgga acgaatccat tttgcaaatt atcaaaccat
    23101 ctctaactgg tcaagcagga acagaaatcc ggaagaaatt ccaatgaaat ttttttatcg
    23161 aatcggaaaa gaatatggtg ttgatccgat aaaaattttc gagaagacca tgatttatta
    23221 ctatcatgga acacctaacg aagtgttaga acatcttttg gaagaggcaa aagaaaaatc
    23281 atcaatatta gagctatcgc aaaatgacaa agttattcaa gtgaaatgcg ttgatccaac
    23341 aggaaagatt attgtcggtt ttgatattga aagaaaatcc aaaatagatt ttattactca
    23401 attaaaaaag ataaataatt tagatattct aacgcttaga ccagatcatg aacagttgtt
    23461 tatgagtttt aaaaaacaat tgaaagaaat acaaaaaaga tataacaagg tgtttcctaa
    23521 tgcgtgaaaa gctcaaagaa atcagagttt tttggataat taacaaagaa tttatcatct
    23581 tatatactct tggatttctc agcatgatct tgttttggtg gttatattta aaaaagtaat
    23641 tggagagggt caagaggatt tttatcaaga taaggatgtg gaaactgcat gtggaataat
    23701 agattaaaaa caggattgtt attaatagtt atttcctgcg ctatgatgat tggtatgagg
    23761 atacaaaggg aacaatccta ttttgaagtt tctgctaaca acgtaataga aaaatgctat
    23821 tacggtcaac attattggag tgaggaagtc agagaaaata tagatcgtga atatgttcaa
    23881 agaattgttt gggatgcata ttctatcaaa gattatccta aaagtttaac gagtagatta
    23941 ttttacagcg aaaaagataa tcaaaaactc tcagatttga tgatgaaaaa agtaaggaaa
    24001 cttgcacaaa gctatttgga agaaaaagca ggagtaataa aagataagga ataaatggag
    24061 aaatttgcta ttgaatgaag aaacggaatt atttttaaaa aaagtgaact ttcttgtaga
    24121 aaaatatttt ttaacaaagc acaaaattat gtatctaata aaaggattga tcggtggaat
    24181 tattttatgg atacttactt tcttttcttt atttggtgga tttactaatt ataaaaagtt
    24241 agtagacgaa aatgtgaagc aaattagtgc atctgaatca ttaagacatc aagctgaaga
    24301 tttagtaagt aatccattat ttataggagg tatggtagaa gtgataataa ttagcttcgt
    24361 tattggttat ttaggtatgt gtatcttcca gataatcttt gatctaataa gaggaaaagt
    24421 ctttcaagaa tttaaatctg aaaaaataaa tttacgttag agataatagg ctatgagagg
    24481 agaagaatag tggaacaaac taatcatatt actgaagaaa ctaggaaatt catttgttta
    24541 gaatcgtttt attcggaagg taggtattgt aacaaaggtg aaacttatac ggcatatcct
    24601 attgaagggg gatttaaatt agtttttgaa aacggagaca tgaattttac cactgagttg
    24661 tttgagtgtg ttcttgaaac gtggtcagat gtgttgcttg aagtcactaa ataatcaatg
    24721 ttatctagta gaattaagca actaaaaaag aaagaaggaa gaaaatatgg cgtatgaaaa
    24781 tttacaatta gtagtagcag gagttagtgg cgatatatat cttgcaaaag caaaagatgg
    24841 aattatggac acgaactata gaagagttgc tacaaatgac gttttagtag caacgacaga
    24901 atggttcatt agaaataaca aaaagttaat ccaatttgaa gcagatgaaa atggtagaca
    24961 tagcttgttt tatacaaacg atcaaaaaaa agctgatcgg atcatagcga ttctaaagga
    25021 gaatgatgat ggtggacaaa gagattaagg gcgaaataag attatttcaa agagaaaatg
    25081 acgattgggg tacggaaata tcaaagaatc ttgctgaaga tatagagtta cgattgagct
    25141 ttataagtat gataattgaa agttttgctt ttgagcttgg tataccgcaa gaaaagttag
    25201 cagaaatact gattaaagca agtaacaata gtcctcagca ctttcacatt gatctaggta
    25261 cagtaaatta gtctttaatc attaatatta atttttttga taagctgatc tgattaatat
    25321 ttcttaatgt aatcattcca atattgatct tcagccgtta acggaatata tagaggaaag
    25381 cggtgaaatg aaatgtctga aaatcatttg aaacgattat cgaaaggaac ggcaagtatg
    25441 tttgatgcca atctactaat ctttaaaagc ctgctagaat ttgaaaaaca acgtgtagaa
    25501 gatattgcta agtatggtaa ctatgctact gttccgagtg tttacaatga tttaatgaaa
    25561 tggttcaagg tacaaacagg gttggaatat agccaacaga atttagtggt aaaaatagag
    25621 gagtatgaaa atcatggcta aaagtttatc acaagaatta agagaatttg cttttagaga
    25681 gtgatgatcc tatagctgaa ctagatcaat tagcggaaag agcaaaagaa ttagaaatga
    25741 tttctggtgc gtatagaatt atttctgaag aattaggtca agcggttgtg tcacaagcac
    25801 ttgaaaatta tttgaaaaaa caagttgatt agtgacagaa ataacggaat aggatataaa
    25861 tgatggattc aagatgatat tagttaataa aaaatagtat ggagggcaac taattatgcc
    25921 agtaaaaaca aacactgaat ggttagatat atgtgatgaa ctatttgata ctaaagacaa
    25981 gaaagaactg gcgatgcgtg tagcatatgc tatagttcaa aaagataaat attttgctgt
    26041 tccatctaca gactatgaca ttgaagtgat agttgaccct attactggag ataagaaatt
    26101 acgagtagtt attgagtgta acgcctaatg tattctttag aacaatagga ggaatcttat
    26161 ttcttaaaag tgcaggaatc atatagaaga gtggttgcta accgtcttaa gtatattaga
    26221 aagagatgca aatttaatgg atatttttga agttaaacct gattcaggaa aatttattgt
    26281 tttgcaggaa aaaagagtga ttgcaattga aagaacagaa atagaagcat ataaaaagat
    26341 acatgaacta gcagaacaat atattgaaga tttgaacaat aattttcaag aatttctaac
    26401 gcaaaatgaa taggcaggta tagacagaga taacaaacta ggaaaggaag accataaatg
    26461 aaaatttttg aaattgatga cccgtatttt gcacttatag tagctgaaaa cgaagaaaaa
    26521 tgtatggata tatatgaaga agtaatttgt gatgtagaag gcaaagaaga atttttacct
    26581 tgtataaaag aacttgaaac gacagttgcg attacaaagg ttgcaaatgc agtgagtaat
    26641 gaaacaaacg agctagtagg aattcatgaa gctggtaatc ttgtgtttag ttgtcttaat
    26701 agcaataaat ctgcattatt ggctcttgat ggggatttat tttaatccaa caaccaccaa
    26761 aatcacctaa aatggtcgat ttgaccagtt tggataaata attgtgtaga tcaattttgt
    26821 aaatgaagat gttagataaa ctagacatca atctgtatag ctaaagaaaa gagaacggta
    26881 aattatatcc gctctctttt ctttagctat ttatagaata taccttctct aaatccttcc
    26941 atcaatgtga gcttgcttaa ttcatttgaa attaaaaaag ttattgctat tatacacatc
    27001 gttgtgtata atataaagtg taaatataat tgttcctttg agctattcat tagagttgaa
    27061 gcatagaata gtttcataga atccatagta aaaaatttga gagtaaagga gaagttatga
    27121 gcgaatttga atttttagat aaaagtattg gttctgcttt tttgattgat cctgatctgt
    27181 ttggagcatg ggctcaatca tcaggttata attgggcttt tagtgatgat atggtcgagc
    27241 ttttaagtca aacgatgaat tgggaagctt ataagatcaa gcaattttta gatgttgttg
    27301 cacagccgat ttatacatct gctgatgatc attggtcagg tagagagttg acaacactca
    27361 aagaactagc ggagtacaat aaaagataca aaattccgtt tcaagtttct tacatgtcag
    27421 atgatttttc cgttatttac tcaataggta aagaaagcag atatttaatc cctaataggc
    27481 acttagagat aaagatgaat agcgattata ccgactatac gctaagtgag ttaaaagctc
    27541 ttagacagag cgacagtgag gaatttagcg cactcaaaaa tgaagtgagt gcagataatt
    27601 ttcgcagatc agatattgaa gaactgaaaa aaaagcaaaa acagcaacta gaaaagcaaa
    27661 aaaaattaat tgaagacatt gaatataaac gagtagaaga gcttgcaata caagaagcca
    27721 aaattgaaaa aatgcgacaa gaactctata aaaaacagca gtcaatgtta gcagaagcaa
    27781 ataaaatgcg gtttgaatta gagaaaaaag tagaagattt gaatactaaa attttttatt
    27841 tggaaactga gattaatgca atcgaaggct attttggtga gagtacaact tttgttcagt
    27901 tgcgttcagg tcaaaagagc ttgccggaag aaccagtggt gatgtttcaa aaagtacgat
    27961 atctagatga agaattagga agatatttca gtatgcgaga agttgaaggt acagctgata
    28021 ccatgctgac ttttgagcag gcgctatcct caagagatga tttgtttgat ttattctcac
    28081 cgaacaaaaa gtcaatctca atgatacgta tttccaaatc aggatatggc tacactaacg
    28141 gtcaaatgga aagtacaaac atgctagaaa agtataaaaa atatcatgga gaaactttag
    28201 caattttact gagaaacggt gataacttat ggatttgttg gttggatgaa aataagatca
    28261 gtttcaaaga tgatttgttt taccaaaaca aagtaacaga gcaggcatct gaaaccaaaa
    28321 atatggtaaa ggagatagat tggacacata ctcgtaattt agaagagcga ttgaaagatg
    28381 ctaaaaagca gaagaaggct gttagaggag agtttgctag tcgttatatt cttttttcaa
    28441 ttttgcaagg tgttctagat aatggaacga tgatgacctt accagaaaaa gtaaatgtga
    28501 tccaagcaat taatcagaaa agcccatatt ttattgcaag cgtagcggat ggatggttag
    28561 aagacacacg cttgccgtca tttagcgagt tgattgataa atacagtttc tataaagaaa
    28621 caggtgaaga cagagtatta gaagcggaat atgagttgaa tcccgataaa tttgaaaata
    28681 tgagagatta tccacacaaa caaaatgatg acatttatgt gttataccgg ttacgtgggt
    28741 ggtcgcctac atcagcaaat tacacttatt acaacagtga taaccgttca aatagtgaga
    28801 gtggacgtga cttagcatat tcgattgact tgaaagacgg catttatcag atcaatttag
    28861 taaaacaggg aaaattccaa aaacaaatgt tttcccgaac gtttgttgga gttgattcta
    28921 ctacaggcga aaatgtctat gattactatt atgaaagaga aacagagtac tatattcgag
    28981 gacaaaaagc ctacaacaaa agtgactatt ggggtgacaa ggcgaagcga ttaactgcaa
    29041 attttagagt gtatgaatcc gaatttataa acttaacctt tcttaatagt gtttttattg
    29101 attatgtcat tactacgaaa cgtattggaa gttattcaag tacctacgca tccttagttc
    29161 caatgttaaa ctcgatgagc cgtttcttaa aagaaagaga agagaaagag ctagagtata
    29221 tcaaagaaat tatgccagat tttgaaatgc acgaatggca aattgcttta tcagattgga
    29281 aattgacgaa taaggttcgt agaataacac cttttcaagc caagcgcttt ttgaaagcta
    29341 tgaaatttgt tcaatagaaa aatccaccag acaagctgat ggagactaac ccaattacta
    29401 acagttgtaa ttggtgttag cttataaatt aattatggtg tttagtcaat cccaaattgt
    29461 gaaacggcac caataattgg aaaggaaaaa tgaaatgtct gaagatttac gagagctgaa
    29521 gttacgaaag aagcagtatg accagtattt ttccggtttg ttgaataaac atattgaaga
    29581 aggacttgat catgattttg gaaaagatga atattgtttg tattgcgagc gttacaatga
    29641 aaaaatgtct gaatatctac agctgaagaa agagatacag aaattagaga aaaaagtaca
    29701 tgcttggaag tataaagatt ccatgtccgg ttatggtgaa tctcaaaata agccaagaaa
    29761 aaagaagcaa gcgtatgttc taaagatggt aaatggggaa gagttaattg tattagggta
    29821 cagtaaagaa gaggttcgag aactcgttga aacacctgtc aggaaaaagg tagaaacaat
    29881 aaatgagata gacgacagcc aatacaatag catctatatt gaaacaagtc atggtggata
    29941 taagaaatta gcaactttcc ttagaagttc taaagttggg ataatttcta aaaaagacat
    30001 aagtgctgtt accgatagtt tttgtacttt atctgttcct aaagcatatt ttcaatacaa
    30061 cagtaataga tttgatcgct tggagaaatt ctgtagacca tatactgtat tgaaaactaa
    30121 aaaattcaat aaaactaaaa gagtttcaat caagttaccg actggtcaaa tctcgctagg
    30181 cgaaaaagat tatctgataa aagtaggaaa agagttaata atcaaagtgc cggaacgctt
    30241 atttaaagaa aattttgaga tacgtgaaac cttggtatgg aaagaagaat aatagtttgg
    30301 aagacatagc agggtataag gaggaattga aatggcagta aatagaaaat tgcaggtatt
    30361 gattacagac gactttggcg gagcagagtg gtttgatgtg cagtctatag attttgaaga
    30421 agaagtcgta gttatagaaa atgtatacgg cgaagaatac tcatattcgc ttgaaggaga
    30481 gaatgttgag gatttacggt tgattggtga gtagaaaata gcagaaatat cggctctata
    30541 atttatggga ctgatgaatc agaattgatt cttcagcccc attttcattt taggtattaa
    30601 aaaacatatc gttctctagt atgattaaat caccacaaaa taaacaactg agaggacgat
    30661 acgctatatg aatgattcta tcaaaaaaat gctgcgatta a
//
LOCUS       DACESX010000019        30589 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.019, whole genome shotgun sequence.
ACCESSION   DACESX010000019 DACESX010000000
VERSION     DACESX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.019"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            141..1010
                     /locus_tag="IXF88_000734"
     CDS             141..1010
                     /locus_tag="IXF88_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ1507482.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1219..2244
                     /locus_tag="IXF88_000735"
     CDS             1219..2244
                     /locus_tag="IXF88_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1507483.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2283..3233
                     /gene="manA"
                     /locus_tag="IXF88_000736"
     CDS             2283..3233
                     /gene="manA"
                     /locus_tag="IXF88_000736"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1507484.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3327..5030
                     /locus_tag="IXF88_000737"
     CDS             3327..5030
                     /locus_tag="IXF88_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1507485.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5032..7065
                     /locus_tag="IXF88_000738"
     CDS             5032..7065
                     /locus_tag="IXF88_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1507486.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7076..7516
                     /locus_tag="IXF88_000739"
     CDS             7076..7516
                     /locus_tag="IXF88_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1507487.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7716..8843)
                     /locus_tag="IXF88_000740"
     CDS             complement(7716..8843)
                     /locus_tag="IXF88_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1507488.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8846..9523)
                     /locus_tag="IXF88_000741"
     CDS             complement(8846..9523)
                     /locus_tag="IXF88_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507489.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9787..11553
                     /locus_tag="IXF88_000742"
     CDS             9787..11553
                     /locus_tag="IXF88_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1507490.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11568..12005
                     /locus_tag="IXF88_000743"
     CDS             11568..12005
                     /locus_tag="IXF88_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1507491.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12018..13169
                     /locus_tag="IXF88_000744"
     CDS             12018..13169
                     /locus_tag="IXF88_000744"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1507492.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13231..13929)
                     /locus_tag="IXF88_000745"
     CDS             complement(13231..13929)
                     /locus_tag="IXF88_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1507493.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13922..15274)
                     /locus_tag="IXF88_000746"
     CDS             complement(13922..15274)
                     /locus_tag="IXF88_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1507494.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15497..16897)
                     /locus_tag="IXF88_000747"
     CDS             complement(15497..16897)
                     /locus_tag="IXF88_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1507495.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17058..17795
                     /locus_tag="IXF88_000748"
     CDS             17058..17795
                     /locus_tag="IXF88_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1507496.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17920..18399
                     /locus_tag="IXF88_000749"
     CDS             17920..18399
                     /locus_tag="IXF88_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1507497.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18396..19163
                     /locus_tag="IXF88_000750"
     CDS             18396..19163
                     /locus_tag="IXF88_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507498.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19393..19962
                     /gene="lepB"
                     /locus_tag="IXF88_000751"
     CDS             19393..19962
                     /gene="lepB"
                     /locus_tag="IXF88_000751"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1507499.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20066..20668)
                     /locus_tag="IXF88_000752"
     CDS             complement(20066..20668)
                     /locus_tag="IXF88_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1507500.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20774..21243
                     /locus_tag="IXF88_000753"
                     /pseudo
     CDS             20774..21243
                     /locus_tag="IXF88_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21349..21933
                     /locus_tag="IXF88_000754"
     CDS             21349..21933
                     /locus_tag="IXF88_000754"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1507501.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21936..23009
                     /gene="prfA"
                     /locus_tag="IXF88_000755"
     CDS             21936..23009
                     /gene="prfA"
                     /locus_tag="IXF88_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1507502.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23002..23841
                     /gene="prmC"
                     /locus_tag="IXF88_000756"
     CDS             23002..23841
                     /gene="prmC"
                     /locus_tag="IXF88_000756"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1507503.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23834..24013
                     /locus_tag="IXF88_000757"
     CDS             23834..24013
                     /locus_tag="IXF88_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507504.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24035..25060
                     /locus_tag="IXF88_000758"
     CDS             24035..25060
                     /locus_tag="IXF88_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1507505.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25235..26479
                     /locus_tag="IXF88_000759"
     CDS             25235..26479
                     /locus_tag="IXF88_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1507506.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26632..27579
                     /locus_tag="IXF88_000760"
     CDS             26632..27579
                     /locus_tag="IXF88_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1507507.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27713..28342
                     /gene="upp"
                     /locus_tag="IXF88_000761"
     CDS             27713..28342
                     /gene="upp"
                     /locus_tag="IXF88_000761"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1507508.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28481..28723)
                     /locus_tag="IXF88_000762"
     CDS             complement(28481..28723)
                     /locus_tag="IXF88_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507509.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28844..28916
                     /locus_tag="IXF88_000763"
     tRNA            28844..28916
                     /locus_tag="IXF88_000763"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28877..28879,aa:Thr,seq:cgt)
     gene            complement(29035..29673)
                     /locus_tag="IXF88_000764"
     CDS             complement(29035..29673)
                     /locus_tag="IXF88_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1507510.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29796..30428)
                     /locus_tag="IXF88_000765"
     CDS             complement(29796..30428)
                     /locus_tag="IXF88_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1507511.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT        10410 a         5378 c         6178 g         8623 t
ORIGIN      
        1 agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct cacatttttt
       61 ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa taaagcttga
      121 cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc tatttaaaaa
      181 gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag aacgttgttg
      241 gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa gaagctggaa
      301 gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa
      361 agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat atcctttgtc
      421 gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga cgtatgggga
      481 ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag aataagaacg
      541 accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa tctgaagaaa
      601 aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat gttactaact
      661 taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat aatgaagaag
      721 agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa gctagcggag
      781 gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa ttcatgatcg
      841 atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt gatttcttct
      901 tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa tggtggttcg
      961 tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa ctttttcttt
     1021 cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc ttattctaga
     1081 gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc agtttttttg
     1141 tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa gaatctgata
     1201 gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa aaagatggaa
     1261 gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt agaaacattt
     1321 acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga tctgttgttt
     1381 gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg tctattagaa
     1441 gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag
     1501 acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg gataccaaac
     1561 agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga accaggtgag
     1621 caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg gttgatgcta
     1681 gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt ggaaccatta
     1741 ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa
     1801 ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag agttttggct
     1861 gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac ttttgtccaa
     1921 atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga gacgattgat
     1981 tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg atgggactta
     2041 aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac aggtacgttt
     2101 ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct
     2161 gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa
     2221 gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga ggagtttgaa
     2281 ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc
     2341 gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa gattgggcga
     2401 tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat
     2461 tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa ccagtgtttc
     2521 cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta catccagatg
     2581 atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg
     2641 atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga gaagaactag
     2701 ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca gttaaaaaag
     2761 gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga atcatgattt
     2821 tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac agaaaagatg
     2881 cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca actgtccctg
     2941 cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg atcgtcactt
     3001 atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc acatctttca
     3061 aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac cttgtcgttg
     3121 acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat ggcgtttctg
     3181 agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa tagttcccag
     3241 atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa atccgtccat
     3301 caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc
     3361 gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg atcatgccaa
     3421 atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt gtgtctggcg
     3481 atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt ttacccatct
     3541 tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa
     3601 ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga gcgatgatta
     3661 tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat gtgaaattca
     3721 aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt agcgttattt
     3781 gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg ttgctcgcct
     3841 ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg atcttagaac
     3901 ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca cctttaggaa
     3961 tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca aatcctggac
     4021 ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa aaagtaaatg
     4081 cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat tttccatttg
     4141 tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc ggcacgttga
     4201 tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca ctactcgtga
     4261 tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata gcaatcagta
     4321 catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa gaaaaagaga
     4381 acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat tccgtcattt
     4441 ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat
     4501 tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa ttgaataacc
     4561 aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac
     4621 ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg ctgtcacgaa
     4681 taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca gatatcacag
     4741 aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga cgcggctcac
     4801 aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta
     4861 tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa
     4921 gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt ctagttgaac
     4981 attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa gatgtattta
     5041 tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc tgtatctatc
     5101 caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga attagataat
     5161 cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag aggctcattt
     5221 gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga tgggaaaagt
     5281 aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt actgacgaca
     5341 gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac cactttttat
     5401 gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat cattcggaac
     5461 cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc gaatgtatta
     5521 gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc atcactgaat
     5581 tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt aatcggggaa
     5641 gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt ggttttgatg
     5701 ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga gacatataat
     5761 agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg gatcgcttct
     5821 gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt attaaatgac
     5881 ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca gcttgcttat
     5941 ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt ttatgaagat
     6001 gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag agccgtttta
     6061 caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata cgaggaagtt
     6121 gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt atccgaagaa
     6181 gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc
     6241 aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta
     6301 cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta
     6361 aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga
     6421 tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta
     6481 aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat
     6541 aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga tcaaatcaat
     6601 caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg
     6661 attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat
     6721 cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg
     6781 ttattccata catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa
     6841 catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt
     6901 atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga
     6961 gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt
     7021 tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat
     7081 acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt
     7141 gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc
     7201 aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca
     7261 tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga
     7321 tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc
     7381 tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat
     7441 cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa tgatttttga
     7501 acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac
     7561 ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat
     7621 gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac
     7681 aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata
     7741 gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc
     7801 atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt
     7861 tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa
     7921 gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga
     7981 gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac
     8041 aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg tatctcctga
     8101 ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt
     8161 atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta
     8221 aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag agagattacc
     8281 tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc
     8341 aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agagagatgc
     8401 gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac
     8461 tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga
     8521 tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg
     8581 ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat
     8641 tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta
     8701 tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca
     8761 aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg
     8821 cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa
     8881 tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag
     8941 aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt
     9001 tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca
     9061 acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat
     9121 gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca
     9181 atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca
     9241 aataatcaac agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag
     9301 accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag
     9361 ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg
     9421 caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt
     9481 ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg cctccaatat
     9541 aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca aaagttattg
     9601 tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac
     9661 cgaaatcttt ttattttgtt gacatactca aacctttggc acaaagtttc gtgccaaagt
     9721 acgactattt ctctttttag taagcggata caattagagt atcaaaagga gagagggaga
     9781 gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa
     9841 ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt
     9901 gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt cgctaccatg
     9961 gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta
    10021 attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga
    10081 acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc
    10141 aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat
    10201 aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt
    10261 ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc
    10321 catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat
    10381 gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa
    10441 tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt
    10501 actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt
    10561 ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta
    10621 tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta
    10681 agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct
    10741 taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca
    10801 gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag
    10861 acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc
    10921 tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc
    10981 gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa
    11041 gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa
    11101 gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat
    11161 tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct
    11221 gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag
    11281 attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa
    11341 gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta
    11401 aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg
    11461 gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa
    11521 tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc
    11581 cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt
    11641 gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat gccatggtag
    11701 atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag
    11761 atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg
    11821 tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt attgcaggag
    11881 tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt gatatggaca
    11941 atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat ctagcaattg
    12001 cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc ggtcgaggat
    12061 ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat gtgaatcaca
    12121 cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca gatgaatcaa
    12181 aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag
    12241 taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg aatatcttac
    12301 cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa
    12361 catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag
    12421 aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc tttccaaatg
    12481 cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc gtccaagtgg
    12541 aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa atccaattaa
    12601 aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta tttagtgtca
    12661 atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag accattgatg
    12721 aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag gaaacaggaa
    12781 gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat attgaaaaaa
    12841 tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc gctcggacac
    12901 cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa ctacaagaac
    12961 ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat tactatgatc
    13021 ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac ttggaacaat
    13081 tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg
    13141 tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa
    13201 atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa gatagggcag
    13261 atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag ctgccaggtt
    13321 ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc ggtaaatgac
    13381 accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc ccaaaggtcg
    13441 ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat aagtttcatt
    13501 gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct gacttaacgt
    13561 gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca tgtagtagtg
    13621 acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt cgatatagtt
    13681 gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct caaagtcttg
    13741 accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat gtatactaac
    13801 gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca tcaaaaggtt
    13861 tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat tgagttcata
    13921 attagccacg gttcattcct cctttgataa atccttgagc agctagttct ttacgaagct
    13981 gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat tctttgattt
    14041 tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga atcccggcaa
    14101 cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca gctgggatat
    14161 ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg attccgtctg
    14221 cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga ccaatcatat
    14281 cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat tttttcccat
    14341 tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa ccttgtttga
    14401 ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa gcattgtaga
    14461 cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg aaatctgctg
    14521 acacattgtc catccgtacc atatcagtta atcgataatc caattcagga cgtttgaagt
    14581 cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta taatgcaaga
    14641 tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt tgttcacgat
    14701 cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata gaattgaaga
    14761 ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca ccgtctacaa
    14821 tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg aaaatatttg
    14881 taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt gcttcatcta
    14941 tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag aaagtcgata
    15001 caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg aattgctggc
    15061 gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg gtgacaacga
    15121 cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc agcgctagtt
    15181 tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc gacgtttttc
    15241 caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa acgatttgct
    15301 gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt gcttttctta
    15361 ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac
    15421 aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat tattccctct
    15481 tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca tagcctagat
    15541 ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc cgcccaataa
    15601 acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta atccctaaag
    15661 ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc agcgtttgtt
    15721 cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa agcataaaga
    15781 caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata ttgatgttat
    15841 cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta gttgttcgaa
    15901 tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa aaaatgatca
    15961 gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta cgagtcgtaa
    16021 tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg aataaatggt
    16081 ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca aaagctaaca
    16141 cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa cgtttgtata
    16201 atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa
    16261 acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa tccccagtga
    16321 cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa aaaacggcat
    16381 cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa
    16441 gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg ataccagaca
    16501 aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt
    16561 tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca acttgaaata
    16621 atatttccag cagtataata ccgttatcat tgaacacaga attgatatca aaagtcgata
    16681 gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa gaaacatggc
    16741 tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc gtcatcaata
    16801 tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc
    16861 gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc tgacaatagt
    16921 cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca aacagataac
    16981 aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta gaaaaaagaa
    17041 ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa
    17101 aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa
    17161 agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca ttggaattca
    17221 tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat cctaaaagtg
    17281 agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat gagaaaactg
    17341 accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc aaaatggttt
    17401 taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata
    17461 tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac tttggaagtg
    17521 ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga atggatatcc
    17581 aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac
    17641 gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt ggttgcgtgt
    17701 tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca aaagcttact
    17761 taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga
    17821 aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc gtagtaaagt
    17881 aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt agcacctatt
    17941 ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac aggtattgtg
    18001 aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga aacaacacca
    18061 actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga aaccactacg
    18121 acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata tacaactgac
    18181 agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc aaccaatcca
    18241 agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac gactgtagat
    18301 gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat
    18361 acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta gaccttatca
    18421 acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt aaccgtggat
    18481 atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat ggactttggc
    18541 aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg cttgtttatt
    18601 ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc gatgtcactc
    18661 agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa gaatcgatag
    18721 ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct tatgacgaag
    18781 attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt gagatcaaaa
    18841 caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg tatccttatt
    18901 actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga agctactcgg
    18961 acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg gaacctgtcg
    19021 gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat gagccttatc
    19081 gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt tatagtcgta
    19141 gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa
    19201 acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt ttttatataa
    19261 cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa cattgtagaa
    19321 taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga aaatacaagt
    19381 aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct tgtattagcg
    19441 gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg
    19501 gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa ctatgaacga
    19561 atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt taaacggatc
    19621 atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat taatggcaaa
    19681 aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga taagttacaa
    19741 ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc cacctttacg
    19801 agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga taaccgcttg
    19861 atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca aggaaaagtt
    19921 gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag
    19981 ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat ttcttggagt
    20041 tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag aaaagcgaag
    20101 cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta gcagagacaa
    20161 agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta tggactttcg
    20221 gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg ccaggagcta
    20281 aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa agtacaagtg
    20341 atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca gcgtggatcg
    20401 ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc agcatagttt
    20461 ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa ctaacaagcc
    20521 cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt agataagtag
    20581 ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca taatggttag
    20641 cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac tttgaaattt
    20701 taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca aaaagggaga
    20761 atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag gaaagtgtgt
    20821 ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa cggaaaaata
    20881 tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag aatatttagg
    20941 ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta ttgtatggga
    21001 ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga agcagaaggt
    21061 tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg gcactactct
    21121 atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt ggaagttttt
    21181 ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt ggaacaaaag
    21241 tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta aaaaatgctg
    21301 aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat ggcacaacta
    21361 ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa agtcgcacat
    21421 aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga tacaagagat
    21481 ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc gatatttgaa
    21541 gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg tgtgctgatc
    21601 gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat tgtggacgaa
    21661 ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga gttatttgaa
    21721 ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac aatctgttgg
    21781 ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc tgtttatgaa
    21841 ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg aaaacattac
    21901 tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta caagcaattg
    21961 aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt tctgatacaa
    22021 agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc gaagtttatc
    22081 gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt ggtgaaaaac
    22141 tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa aaagaaaaag
    22201 aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat gacgataaaa
    22261 atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta ttcgctggcg
    22321 atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca gaagtgatgg
    22381 aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc accggagaca
    22441 acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt gttccttcaa
    22501 ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg ccagaagctg
    22561 aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat catgcttcag
    22621 gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact cacttaccaa
    22681 cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt gaaaaagcaa
    22741 tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa agcgagtatg
    22801 atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc cggacgtata
    22861 atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa aaattagatc
    22921 agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat gatcaaacat
    22981 caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct gtcacgggct
    23041 tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt atttttggaa
    23101 cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat ttcagaagaa
    23161 gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc acctcagtat
    23221 ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc aacactgatc
    23281 cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc tggtgtaccg
    23341 ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt gaaagcagca
    23401 agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga agtagcgaaa
    23461 gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac attaggacca
    23521 gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag tcggaatgaa
    23581 tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct ttttgctgag
    23641 aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt actaaaatca
    23701 aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa aaaaatattt
    23761 cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg caatgaacga
    23821 atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt tttttattat
    23881 aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac aagtttatcc
    23941 actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac tcagacaaaa
    24001 taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat atgatgaaac
    24061 agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt ttcctaccga
    24121 aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga aagtgttttc
    24181 tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt ttgaacaagt
    24241 aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga acttctggcc
    24301 tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag ttgtgacggg
    24361 aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag aagtaatcga
    24421 actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac caagtccaac
    24481 gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta ttgatgatgg
    24541 agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa cagccatgcc
    24601 tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca tcgaatctcc
    24661 tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc ctgggatgaa
    24721 atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg attggtcaac
    24781 tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa gccccgaaat
    24841 tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca actcagtaga
    24901 agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa cacttggatt
    24961 agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt acatgaatcg
    25021 cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt attgtcgcat
    25081 tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga aaaactaagc
    25141 gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga tgggaaaaat
    25201 aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa cgtttgaccc
    25261 tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt tggagctgat
    25321 tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta tcttgacaaa
    25381 taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat ttgtcgatat
    25441 cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat ttgcgaacgt
    25501 ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg tagaaccagg
    25561 ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg gatcaccagt
    25621 caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga caacagaagt
    25681 tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat tgatcgtagc
    25741 aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa tcgcagatga
    25801 agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag cagcaggact
    25861 tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata aaacattgcg
    25921 tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa aaataaatag
    25981 tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg gtaaagcagt
    26041 agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa tcatcgccaa
    26101 tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca tttcaggggc
    26161 gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg gaaaagaagc
    26221 agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc cttttgaaac
    26281 actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca ctactcgcgg
    26341 gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat tacaagcaaa
    26401 agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa ctgaaaaatt
    26461 tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt tcaatgttgg
    26521 gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga tgctctctag
    26581 tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt catgactgat
    26641 cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc tcatccgcgt
    26701 atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca agaatcacct
    26761 gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga atggggaaaa
    26821 ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt tattttttat
    26881 attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt gatcagagaa
    26941 cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc accagaagca
    27001 aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa taaattagcc
    27061 aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt aggtggtaac
    27121 atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat atccaagcaa
    27181 ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca tgaattcgga
    27241 gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata tacgacgaat
    27301 gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa tgaactaaat
    27361 gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga tgaaggtgag
    27421 gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg cttccaagga
    27481 atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac gcgtgcagca
    27541 atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt ccgcaggaaa
    27601 acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag tcaatcactt
    27661 gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa aaatgggcaa
    27721 atttcaagtg atcgatcatc cattgattca acacaaacta actattattc gggacaaaaa
    27781 ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga tggcatatga
    27841 agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca ctgaaacaac
    27901 acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg caggtattgg
    27961 aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata tcggtcttta
    28021 tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag aagacattga
    28081 tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag caatcatggc
    28141 aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt tggtagcagc
    28201 acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct atacagcttc
    28261 tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg atgcgggtga
    28321 ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac ttttgtcaca
    28381 tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc tttcgtttgc
    28441 atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt tttagttctt
    28501 tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt tctgcagttt
    28561 catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca gtttgcgact
    28621 tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca atttccgatg
    28681 gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct gcctttcaca
    28741 tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc catcggactc
    28801 tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct tagctcagtt
    28861 ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag ctggcattaa
    28921 aagagaagac agatatatca aggattttta atccattgat acatctgtct ttttgtttaa
    28981 aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa aaatttagga
    29041 gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg cccaacttgt
    29101 aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca ccccttgtat
    29161 caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac tgtaatggat
    29221 aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact tactagaaaa
    29281 aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag gcaaaataat
    29341 aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc atttcaaaat
    29401 ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa aatatttggc
    29461 tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt ttttaccaaa
    29521 aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa taatggatgg
    29581 cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata tccctttttt
    29641 aagttctatt ttttttccta aaatgttatt catgataata actcctacta ttcagttttt
    29701 gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta tggatatttg
    29761 tattgctaaa aatttatctt atatattcat tttgattata ctacttttat ttgtaaaaat
    29821 tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag atgttccgag
    29881 cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa atcagtatca
    29941 aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag ggaacagtct
    30001 acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat gtttgaacca
    30061 gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat ataactttcc
    30121 atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa catgcttttt
    30181 gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat ctccattctt
    30241 tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat aattggatgg
    30301 cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag ttcaaaatct
    30361 tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt aactttagga
    30421 aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc taaaccaact
    30481 tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt gtcgctatct
    30541 cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaatt
//
LOCUS       DACESX010000020        30397 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.020, whole genome shotgun sequence.
ACCESSION   DACESX010000020 DACESX010000000
VERSION     DACESX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.020"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..414)
                     /locus_tag="IXF88_000766"
     CDS             complement(<1..414)
                     /locus_tag="IXF88_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507512.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKI"
     gene            501..1199
                     /locus_tag="IXF88_000767"
     CDS             501..1199
                     /locus_tag="IXF88_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1507513.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1377..1718
                     /locus_tag="IXF88_000768"
     CDS             1377..1718
                     /locus_tag="IXF88_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507514.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1711..2382
                     /locus_tag="IXF88_000769"
     CDS             1711..2382
                     /locus_tag="IXF88_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1507515.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2388..3074
                     /locus_tag="IXF88_000770"
     CDS             2388..3074
                     /locus_tag="IXF88_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507516.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            3077..3826
                     /locus_tag="IXF88_000771"
     CDS             3077..3826
                     /locus_tag="IXF88_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1507517.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            3838..4107
                     /locus_tag="IXF88_000772"
     CDS             3838..4107
                     /locus_tag="IXF88_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507518.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            4269..4571
                     /locus_tag="IXF88_000773"
     CDS             4269..4571
                     /locus_tag="IXF88_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507519.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            4568..4729
                     /locus_tag="IXF88_000774"
     CDS             4568..4729
                     /locus_tag="IXF88_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1507520.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            4726..5031
                     /locus_tag="IXF88_000775"
     CDS             4726..5031
                     /locus_tag="IXF88_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507521.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            5031..5387
                     /locus_tag="IXF88_000776"
     CDS             5031..5387
                     /locus_tag="IXF88_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ1507522.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            5347..5592
                     /locus_tag="IXF88_000777"
     CDS             5347..5592
                     /locus_tag="IXF88_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507523.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            5589..6008
                     /locus_tag="IXF88_000778"
     CDS             5589..6008
                     /locus_tag="IXF88_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ1507524.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            6005..6562
                     /locus_tag="IXF88_000779"
     CDS             6005..6562
                     /locus_tag="IXF88_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ1507525.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            6559..6855
                     /locus_tag="IXF88_000780"
     CDS             6559..6855
                     /locus_tag="IXF88_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507526.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            6932..7345
                     /locus_tag="IXF88_000781"
     CDS             6932..7345
                     /locus_tag="IXF88_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1507527.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(7803..8078)
                     /locus_tag="IXF88_000782"
     CDS             complement(7803..8078)
                     /locus_tag="IXF88_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507528.1"
                     /translation="MYKPYMISYNLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            8532..8738
                     /locus_tag="IXF88_000783"
     CDS             8532..8738
                     /locus_tag="IXF88_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507529.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            8934..9101
                     /locus_tag="IXF88_000784"
     CDS             8934..9101
                     /locus_tag="IXF88_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1507530.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            9127..9471
                     /locus_tag="IXF88_000785"
     CDS             9127..9471
                     /locus_tag="IXF88_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1507531.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            9476..9757
                     /locus_tag="IXF88_000786"
     CDS             9476..9757
                     /locus_tag="IXF88_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507532.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            9860..10174
                     /locus_tag="IXF88_000787"
     CDS             9860..10174
                     /locus_tag="IXF88_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ1507533.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10152..11846
                     /locus_tag="IXF88_000788"
     CDS             10152..11846
                     /locus_tag="IXF88_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1507534.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            11866..13044
                     /locus_tag="IXF88_000789"
     CDS             11866..13044
                     /locus_tag="IXF88_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1507535.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            13007..13693
                     /locus_tag="IXF88_000790"
     CDS             13007..13693
                     /locus_tag="IXF88_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ1507536.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            13693..14853
                     /locus_tag="IXF88_000791"
     CDS             13693..14853
                     /locus_tag="IXF88_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1507537.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            14863..15738
                     /locus_tag="IXF88_000792"
     CDS             14863..15738
                     /locus_tag="IXF88_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507538.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            15735..16046
                     /locus_tag="IXF88_000793"
     CDS             15735..16046
                     /locus_tag="IXF88_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507539.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            16036..16389
                     /locus_tag="IXF88_000794"
     CDS             16036..16389
                     /locus_tag="IXF88_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1507540.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            16379..16780
                     /locus_tag="IXF88_000795"
     CDS             16379..16780
                     /locus_tag="IXF88_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507541.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            16773..17177
                     /locus_tag="IXF88_000796"
     CDS             16773..17177
                     /locus_tag="IXF88_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507542.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            17189..17797
                     /locus_tag="IXF88_000797"
     CDS             17189..17797
                     /locus_tag="IXF88_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1507543.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            17817..18179
                     /locus_tag="IXF88_000798"
     CDS             17817..18179
                     /locus_tag="IXF88_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507544.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            18182..18364
                     /locus_tag="IXF88_000799"
     CDS             18182..18364
                     /locus_tag="IXF88_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507545.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            18381..21812
                     /locus_tag="IXF88_000800"
     CDS             18381..21812
                     /locus_tag="IXF88_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1507546.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            21863..22600
                     /locus_tag="IXF88_000801"
     CDS             21863..22600
                     /locus_tag="IXF88_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1507547.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            22610..24901
                     /locus_tag="IXF88_000802"
     CDS             22610..24901
                     /locus_tag="IXF88_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507548.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            24925..27051
                     /locus_tag="IXF88_000803"
     CDS             24925..27051
                     /locus_tag="IXF88_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507549.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNLTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            27214..27660
                     /locus_tag="IXF88_000804"
     CDS             27214..27660
                     /locus_tag="IXF88_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507550.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            27662..27799
                     /locus_tag="IXF88_000805"
     CDS             27662..27799
                     /locus_tag="IXF88_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1507551.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            27837..28130
                     /locus_tag="IXF88_000806"
     CDS             27837..28130
                     /locus_tag="IXF88_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507552.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            28127..28351
                     /locus_tag="IXF88_000807"
     CDS             28127..28351
                     /locus_tag="IXF88_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1507553.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            28348..29373
                     /locus_tag="IXF88_000808"
     CDS             28348..29373
                     /locus_tag="IXF88_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1507554.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            29563..29636
                     /locus_tag="IXF88_000809"
     tRNA            29563..29636
                     /locus_tag="IXF88_000809"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29597..29599,aa:Ile,seq:cat)
BASE COUNT        10806 a         4643 c         6382 g         8566 t
ORIGIN      
        1 aattttgtgg atttgggttc tatttagagt aaatatcatt tttgtttggt gattaaggag
       61 tttattaaaa ttaattttcc ctacttcaaa tgcaaggtaa caagatatag cacttttagg
      121 aggaatattt agcggtacag cttcagagta tattctatta tcatcgactg atcctccttt
      181 agcaattaat ttcttaaatg gagtagctga ccatctgcgg tttaagttag cggatgaaag
      241 ttctaaatta actaacatag ctggttctgt tgaatagtta gaaatgatta cttttgtata
      301 aatcatatct gcagcgaagt aagaagcatt aagttcaact tctagttgag ggcgcattcc
      361 ttttattttt atccctgtta aaaacgtact caatataaaa cttattattg acatccattg
      421 aaaaattgtt aagtgaaaaa aattcaaaat aaacaccacc agtttttaac taaattatac
      481 caaaaaggag agaagaaata atgcaagaat tagtaatttt gaaaaataaa gaagctgtga
      541 ctacgagctt acaagtggca gaaagcttcg agaaaaaaca tcaacatgtt ttaagagata
      601 ttgatgcact aaaaaaagat gtgtccaatt ttggacagat gtttgtagaa ggtaatgaac
      661 cagattcata tggcagaaat cgacgagttt tcttcattag tagagatgga tttttcttgc
      721 tggctatggg gtttacagga aagaaagcta tctacttcaa acaaaaatac attgaagcat
      781 tcaacgaaat ggaagatgtt attcgcaaga atactgttcc tcaaacaatt gaagatatga
      841 tgatctatca attagaagaa atgaaagatg ttaaaaaaga tgtttccatg cttaaagata
      901 ctatgcgaat tagcggacaa caagagtttg aaattaagca aaaaggaaat atgaaagtta
      961 tgggagttct agggggaaaa gaaagccgag cttatgaaga aatcagcaaa aaagtattct
     1021 caaaattttg gtctgaattt aaacgtacct tttcaatccc aagatatggc gagttacctc
     1081 gtaagagatt cgatgatgct gtttcattta ttgaaatgtg gttgccagaa actgcgatcc
     1141 gtatggaaat tgatcaactg aacagacaac agagactttt tggtgatgac aatgaataga
     1201 gctgaagcgc taagaatagg gacggcaatt gctaattgct ggtggaaata ctataaacca
     1261 atcatcctaa gccaacaaca tattgacaag caaaaagcat ggcaacaaat aaaaaagtga
     1321 ctccgccggc aagcaaagag tcacaaagaa aacacatcat aaggagattt tagcatatgg
     1381 aaaaagaact ttccactcta gatcaatatt tgattgatcc tgattggggc aagccgaaaa
     1441 ttgaggaaac aagtggtcga aaaatcagac gaaatctttt gacgaatgaa gaactagctt
     1501 gggatcaaga tgatttaggc aaccatgtaa ctatttggga tcatgtttat cttatccatc
     1561 tatcgaagca ttcgaataaa cctgaatata tttacgtcat cgaagatggc ttgattgatg
     1621 cgctagaaga gtacgacaga gataacttga ttgatatctc ttattacgga ccaggtaaga
     1681 aatacattgc tgaaatggag gcagaatttg atgagtgaaa tcaaagggac aacgaacttt
     1741 gaaaaacttt ttagtcgtaa gttaaataaa attctcaaga aaaaaggaaa ttttgattat
     1801 ttatcttggg ctcacgcgtg ggagattatg aaaaagaatg atccacaggc aacggtaact
     1861 attaatgagt acaaacacta cagggttgtt tctggaactc atcaagactt tcttgttgag
     1921 gaatataaac cttttcttat ggatgaaact gggacttatg tatctgtctc agtaacggtt
     1981 aaaggacaca cggaaaccga attatttcct gttttagatt atcgaaacca accagttgtt
     2041 aaaccaaatg caatgcaaat caataactca ttgaagcgat gctttgtgaa agcattggct
     2101 ctacacggac tgggattata tgtatttcaa ggggaagata ttccaacacc acctagaatc
     2161 gatacaaaga aattaaacat gctagagacg attctagaag ctttcaatga gcagatgggt
     2221 aaagatatga ccaaaacctt aatcgaatat gttaatgagc agacagataa attagggctc
     2281 ttagctgata acgttgaaac tattgaacag ttaagctatg agcaatgtgc cttgatggag
     2341 cgagcaatag cagctaagag aaaagaatta gataagaagt gatatgagtg tttaaaccat
     2401 taatcgattc atattcagcg gttctgaaaa agttcaaagg aaaagacata agcgcaacca
     2461 tcaatgagga agtgaacatt gatcgactaa agacgatgta tgacggctac gatggtgatc
     2521 gagtcattga aattcgtttt attgatccta gacgtttcac cgtacagcaa cgaaacttca
     2581 tctatgcgct gataggcgat atttttatcg atacaggcat gccaacggac ttctggaagg
     2641 aattcttcta ctttcgtttt gaaggtgtca cagggcgcaa aataagcctg aaagacgaat
     2701 cgaatacaac tgtgagtgat gccaatgtct tagcaaatat catcttagat ttcatctttg
     2761 aacatcatat tcctttcaaa gaaggctatg agattttacc tgcgaatcaa gaatattact
     2821 tctacaaatg catcacaaaa agagtctgct gcatctgtgg caaaacagga gctgacatcg
     2881 atcactttga caaagcgcta ggaagacgaa agcgcaaaga agttgatcat gcagagtaca
     2941 catttgcagc actctgcaga atccatcaca cagagaagca caaaataggt gtgattaatt
     3001 tcaaaaataa atatcaaatc aaagggatca aattaaacca gaaaacaatc aaaaagttaa
     3061 atattggagg gtaaaaatga cagaacatcg aagttattat gcgattatac cagccaacgt
     3121 aaggtacgac aaaagactta aaccaaatac taagttgtta tacggagaga taacggcctt
     3181 gtgtaatgaa agaggctttt gttgggcagg caatgagtac tttgcagatt tatatggtgt
     3241 gaataaagag accatatcgc gatgggtaag tgatttgatt aagtttggat acttgaatcg
     3301 ggaaatcatt tacaaagagg gtaccaatca aataatcaat aggtacctac gaattaatca
     3361 ataccctatt gacgaaaaac gcaatacccc tattgacgaa aaagtcaaag ataataatac
     3421 atctattaat aatacattta ataatacaaa agaatatata agagagttac cgccttcgaa
     3481 aaaatcgaag gctaagcccg tccgtcataa atacggagag tataaaaatg ttcttttgtc
     3541 agatgagcag atggagaaac tcaaaacaga attccctaat gattaccaag agcgaatcga
     3601 acgactgtca gagtattgtg aatcatctgg taagacttat aaaaactatt tggcaactat
     3661 tcgaagttgg gcaaggaaag aaaaaagtga acctaagaac gcaagcagtg gatacaagcg
     3721 cacaggaaga cgagagaagc ttcctgaatg ggcaatcgac caagaagcct atctcaagaa
     3781 aaaagcgcta gaacgagcta atagacaatc aaaagcacca ttctaagagg tggaaaaatg
     3841 aagatcgatt atctagaact aattaatgaa atagcgaatt ataaaaaggg cgaggaatta
     3901 gacgtcctga gagacgtata tgatcaactc gaagaagctg gaattgaagg aattaagaat
     3961 gatcgttcga gttggagtaa gctcagatac tatttcgcac tttatatcga tacaacacaa
     4021 ttaagaaatt tagcatatac caaattacta tttgttgatt gtgttaaagg cttgcaaaaa
     4081 catcttaatg aacttgagca ggtgtaatca gatggaccta aagacattta cagcacagat
     4141 tgaactaatg catcaagaag ctttaagaca aagtgtatcg tacgaagaca agtggctcaa
     4201 cacgttccat ggcggacgtg agagcgcact tgatcaagta ctcaaattac tgaaaggaga
     4261 atgtcgggat ggataagaaa gcggcaatgc agcgaattat cgaattgact tattcagaag
     4321 attggcaaaa tgacaaagaa gctgcttcag aagtgatgag gcttggaaga gcgatgtggg
     4381 cagacaagag caacaagcca agaccacgaa aaatcgcaat ttggcacggt gacaaacttc
     4441 tagtgatagg gacagctgaa cagttagcaa gtctcacagg cttgcacgag aaaatcgtga
     4501 ggaaaagagc aaggtgtggc tacacagacg ctaagaagag aacgtttaga tacgtggagg
     4561 aatcatcatg acaacagaag aagtgattca aatgcgtatt cgaagccttc agcgtgagat
     4621 tgacgatctg gaacgaacaa aggcagtgat ggtcaatgaa acggcgagaa aggcaatcga
     4681 tttgcacata gagaatttaa gaagggaaat ccatcgattg gaggaatgag cgtggataag
     4741 aaagcagcaa tgaaaagaat tgctgaatta accaagtcag aatcttggca agaagacaaa
     4801 gaaatagttg cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa gcccaaacgg
     4861 agaacgccga gaaaaattgc aatctggcat gatgaccgaa ttttagtaac aggtactgct
     4921 gaacagttat ctgaaattac tggattaagc aaaaacatta tctgggatag agctaggagc
     4981 ttatggattg attcaaaagg acgacagttt aggtatgtgg aggagagata atggatctca
     5041 ttacacaata cagtgacatc atcctcaaga aaatcatgat gaagattcag aaagataaaa
     5101 aatcaaaaga acgagctgaa ttagttaagt tagaaatggc tgaaacagga gcaggagtgc
     5161 gaagtagcag gcattggaaa gcagcagcaa acattgaatt ttattacaac gaaattcaaa
     5221 aagggttcga tcagatgcgt gagctggatc ggcaaacaaa ttggagcaag aaacttcatc
     5281 aagatcgttt caaatttgta gagaagtata aagaaatatt agaagagtat ttgaggagga
     5341 cagcaaatga taaaaaaact cgttcaattc agcatggatt tatatgatat cgaatcagga
     5401 gcaacactat ctgtggaatc ggaccatcta atcataaatt ttggtggaaa gcgccagatt
     5461 attttgtggg tagttgatga tgtactgttt ccagaaattg ttcatgattt cgaagaatca
     5521 aaagcggttg agtttgaaat agtgaaaaaa gtaatggaat tgattgaaaa atacgaggag
     5581 gacagcgaat gataccgaag tttagagcgt gggataaacg aaagaacgta atgagagatg
     5641 tagccgtctt gcattttact aaaaacggca aaacaaactt tattgaatat tggataaatc
     5701 ctaccgaatt gaaatcatat cacgtgcgaa acatcgacct catgcaatcc acaggaatga
     5761 aagataagaa tggtgtggat atatttgaag gggatatagt attagtcagc gtgcgaaatg
     5821 gcttcgatta cttagataat aaagtctgta ttgtcaaaaa ttcaatagat tattccggat
     5881 tagtttgtgc cactgttgat gaagacttag agtatcaaat ttttaacaca gagctgtttg
     5941 aagaatacac gtacgaagtc accggaaata tatacgagaa tagcgagtta ttggaggaac
     6001 agcgatgaat aaacaggaag caatcaaaaa gttagaaagt attaaagcga taggaaatga
     6061 tgcaatagct gcttgctata acgagagtat aaattcaggt attacgttaa tgaaaaaaat
     6121 agacgaaccg cagaaaccag ttattccaca actcgtggcc ggttggcttg agaaatctac
     6181 ggaccctttt acaaaagctg aaaaaatagc gtatttaatc aaatctaaag atggtgattc
     6241 atattatttc tgtgattggt ttgtacgaga tggcatagtg acgcaagagc aaggagaaga
     6301 attactcgct tgggcaacaa gacaatcata tgaaacacta ttgagcctat acaacggcta
     6361 cgaggttgag aaagagccgt tatgggcaat aaagaatgcc gatggaaact atcttactaa
     6421 atgtgcttta tggggaaaag atggagtaaa ttatagtttt gaatgcaatc catctcatcg
     6481 attgcttttc actgataaag caacagcgga tgctgcagca ttgttggtga atggaacagt
     6541 ggaagaggtg gtagaaagat gaaactaaaa gacggatttt acgctagcag tcacggtatc
     6601 ggcggtttaa tgctagatat gccgacaaag aaccctaaaa cacgcaagaa atcaaaattc
     6661 aaagtcggtg acatggtccg ctgtgaagca gaagagttcg tttatccgtt cagaggatac
     6721 gttaaaaaga tactgtcaaa ctcagcaatc attcgtattg aaaatacaat gaagtgtgat
     6781 cgttcgactg caaaacacaa gcattattta gctgtagcga gattagttga catggaagta
     6841 atcaagagca aataaaaaaa gccggatcgc tccgactgat tcaataaatc caacacattt
     6901 attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg tagatttcaa
     6961 acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg agcggatggc
     7021 aggtcgctct ttgatagata ttaagtcgcc gattataacg gatatgccga aggcaccgaa
     7081 gcatggcaat aaggcagaag acgcgattat tcagatgatg gatatagaaa cggagagaga
     7141 tgcgattcta gcggctttga tggctcttag tctgattagt cgtcagatac tctactacag
     7201 cttctgtgtg ccagatagct tctcaaacta cagaattagc cgtgaagtgg gttattcaga
     7261 aagaagtata caacggatga agtcggaagc tctaatagag tttgcagaag catataaaca
     7321 cggaaaaata attgcttata aataatttgg cggttttttg gcggaatgat ggcggttttt
     7381 agacatttac cagtgttatt atggtagtgt cgaaagatta ggaaacagga tcgacaaaat
     7441 aaacgaaggg aggcaatctc cctcatcgtt ttaaacttct tcttagatag acagcaacta
     7501 attatgaaga aaaggatgtg aattcaactc ctcctaaatt cttcatcggt tgctgtttat
     7561 tttgattaga cagcagcact cacaaaacta aacatgttct tgtcgtcttt caactgctgc
     7621 tgtctattaa tttatgtatt ggagggaaga aaaatgaaat tatcagtaga aggcactcca
     7681 gaagaaatac aagaattgct ccaagctatt ggtagtagca aggagcaaaa ggtaagtgtt
     7741 tctaattcag atattgacgg tatttgcaat tgtttaaaca gagaaaaaat atctaatcct
     7801 tactaagaga aaatatgttc gttaatgaat ttccaatttt cctctgtagc tctaccttga
     7861 taattttttt gcagttcaca gatgaataag gaatcgttat tatcaagtac tgtatttaat
     7921 ttttcacaca tttgatttgg attcaaatta gttttaacta accaaaaaga tttttgtagc
     7981 tttatatatg ccccaaattc ttttattatt tcaaatactt tatcatactt ttgccctggg
     8041 ttattaagat tataactaat catgtagggt ttatacatgt tgtcttgcac atccttgttt
     8101 tatatttcag cggaccactc gctgataaat aaaattatac gcttagtatt tattttcaca
     8161 atattaattt atcgtaataa cttttgagta aatagaacaa aaaacctgca ctagtttccg
     8221 ctagtacagg cagtgactat atcggttgat aatctagcat atattaaaaa taattgcaag
     8281 aaaagaaatt tatagtggtt tataccaaat tatcaaagca atgttatttt gttgctgtct
     8341 attgtttttt aattattcac acgataacta aaggtgggtg aagagaaatg attccattaa
     8401 taatttcaat ttttgcgctc tgtcttaatg tctatatgat tggatttaaa aatgggcaaa
     8461 ataaaaaata gtagcagcca agaataattt tatagtgtca ctgtggcgga aagggtagac
     8521 gctaagcatg tgtgctaggt caatgcttcg gcaaccatgc aaggttcgat tccttgccag
     8581 cgacattaaa tgcctatgac ggttacgact accgaaaaaa gatcgttaag aagctatacg
     8641 gtgctacgta cggcaatgta gtaagtgtgc tatctgtaca ccaccaagct tcggtcactg
     8701 tggcggaagt agaagacgca gcggtaaatg gcgagtagcc tcgtgagagc ctggtaagtt
     8761 ctcgtgagtg gtgcaatccc actccagcga ctttaagcaa ccgaggcatc ggcggtttaa
     8821 aaatataggg gtgcgcaatt tcgtacgcgt tttgtgcatc gtgcaagtta ctattacata
     8881 ttagatcact ctttgagtgg tctttttatt tttgcacaaa ggaggaaaca acaatgtata
     8941 gaccacaata cttagaacag aagtatgaag taatcactgt tcataaaggt aatggcgaaa
     9001 aagtatatga gtatagaaga ccaataaaga gcgatacata taaacgaaag gaaaacaatg
     9061 aagttattcc attgtatggc aaaagaatag ctaagcatta aataagattg cgaaaggaga
     9121 cggaacatga ccgaggaatt ctatagatgg ctattacagt tgacaagaga agatcgtttg
     9181 gttaagttct atcagtctcc taaatggcgc aggcttagag agaaagcgat gaaacgagat
     9241 cactatgaat gccaagagtg tagaagacta ggtaagtatc atagagtaga gaacgttcat
     9301 catataaagg aagtcaagga tagacctgac ttagctttag atttagataa tcttatttgt
     9361 ttatgtgttg aacatcataa tgaagttcat ggcagatatc ttacagcatt agataaacaa
     9421 gagaagaaga tagaaagctt tgctaacttc gatgcaagtg aaaggtggta agtgcatgat
     9481 catcaatgat aatggcagag agtatgatac agaaaagatt gaagagtatt catcttatac
     9541 tcagggatta attaaacgtt tgatatacgt tcgctatgta ggtattaggg atctgttatc
     9601 agataactgt tgtagtaaat acaaagtgaa tcaagtaaga gaagcgttga ataaagataa
     9661 taacgtcgaa agaataaaaa atgtttttgg atatagtatt gaagagatta attattacat
     9721 tgacttcgct gaagctttca ttccgatggt gagataaccc ccccttaaaa taaatcgcaa
     9781 attttttggg ggtgatgaaa cggagggggc tgtcaggaaa agagattttt tcgaacttta
     9841 tcatgaaagg agggctaaaa tgtttaaaaa cgaattgtct caaaatcgct acagagaaaa
     9901 attacgccgc tctttaataa gccaattgga aagtcagaaa acaaatattg agccattctt
     9961 agataatgtt gatcgttata tcagtttatg ggaaacggcg atatcactgg aagaagatat
    10021 atccgagaac ggcattagac tggagaatgg taaaaagaat gaatcagtag cgttgcttgt
    10081 ttctgtcaac aaacaaatgg gattgatgtt ggataaactt gccattactc ctgaattggt
    10141 aggtgaagca aatgaatcaa ttcctgagtt ataagcatat tgaaaattgg ttcaaagcta
    10201 tagaagaagg cactatcaag gtatgcaaag agcaattatt gctaaaaaat tatctagaag
    10261 aaagagtctt tactagagaa gatatttact tcgataagca gatggtagag gattcaatca
    10321 atataccagc acaatacttt ccattcgaat taattccgtg ggaaaaattt ctacaatgtt
    10381 ttatttatgg tgttcgatgg aaaaaagata aaacactagt gttcaataga tatctttcat
    10441 taatgggacg tggtaatggt aaaactggtt ttgcttcttg gaacaacttc tttctactaa
    10501 ccgctaaaca cggtattaaa aattatgata ttgatatcta tgccaataat gaaagccaag
    10561 caaagactag ttttgatgat gtatttaaag taattaaaga tcatcctgat ttagataaaa
    10621 aagtatttaa agctacgaag gaagttattc aaaatatcgc tacaaacagc aaacttcgtt
    10681 ataacacggc aaatgctaga acaaaagatg ggaagcgacc aggtgcaaac cgctttgatg
    10741 aaattcacga aaatgaagat tattcaatga taaatgtggc tacttctggt ggtggtaaaa
    10801 ttcgagatta tagagaattt tatgatacaa ctaatggtca tgttcgtggt ggtccgcttg
    10861 atgacattat agaagaatca aaaatgattc tttctggaga acttggaatt gacaaggatg
    10921 gagcagaatt ttctagtttg tttccattta tttgtcgctt ggataacgat aatgaagttg
    10981 atgatcccga catgtgggaa aaagcttgtc caactattaa ttacaatgca gatttaaaac
    11041 ggaaaatgtt tcaagaatac tctcaaatgc aacgtaatgc tggtttaaga cttacgttca
    11101 tgaccaaacg aatgaacaga cctatggaag atacacgatt tgctgttgct tcatatgatg
    11161 atgttctgca tacgaaagaa aaagaatttc ctgaaaaaat ggatgaagtg ataggaacag
    11221 tcgattttgc tgatagacga gattttgcca gcgttgggtt gctaggaaaa tacgataaag
    11281 atgtttattt tacacaacat acttttatcc acgaatcagc ccttcgatta caaaacatca
    11341 aacgagaggt tatagatatt tctatagatc aaggaaaatc acagatcgtt catggaaaaa
    11401 atatagaagc tgattatatt gtaggttggt ttcttgaaat gagtaataaa tattatatta
    11461 aaaaaatcgc tatggatatg taccgtgcaa aaatattgaa gcccgcttta gaagaagcag
    11521 gttttactgt ggaaattgtt cgaagcggat ctgttacaca tggtatgtta aaagatctgg
    11581 ttgatgacct ttttattaat caacgtttat tttttggtga cgatgcgatt atgcgttggt
    11641 attgcatgaa tgtatatgaa gagcatattt ctaatggaaa tatacgctat gaaaaaatag
    11701 aacctgaaac tagaaaaacg gatggctttt tttcattcct tcatggtttg aattttttag
    11761 atgatattta tgattctgct cctgtaacag tcacaaatag ctcagtagaa aatacaggaa
    11821 ctggatttac tcctctagta ttctaacttg aaaggaggtg agaaagtggg gatttttcaa
    11881 aaggcggtag gatacttcac aaaaaaagca acggttcctt tagaagaata cttttgtaaa
    11941 ttgcaagttg attttgtgta tcgaaaattt gcgattgaaa cttgtattga tttgattgca
    12001 aatgcgatga gtaaagcgga attcaagtca tatgaagatg gaaaaaataa aaagaatgat
    12061 ctttactata ggctgaatgt agctcctaat aagaaaaata atgcaacaga atttagaaaa
    12121 aaactgatca ggagattaat attctacaat gaagtattga tcgtttctcc gtctaataat
    12181 tctagcgaaa tatttattgc ggatagttgg gatgtcacag aatatgcatt gaaagatgat
    12241 gtgttttctc aagtgcaaat taacaacata gtccttgata gagaatttct agaaagtgat
    12301 gttatctata taaaatacgc agatcaacaa attaggcaac tagtcgatgc gtattatcaa
    12361 gcgtatggga aactcatttc tagtgccatg aatgtttaca agcgctctaa cgctcgtaga
    12421 tacgtactga aagggaattt attccgaccg caagacaata caacacaaga tcaaatcaat
    12481 aaaatgatga catcacaatt taaggctttt atggaagctg ataatgcagg tgcggtattt
    12541 caattacaaa atgagtacac attagaagat ttcagcggaa acttccaaag caattcaaga
    12601 gatataaaaa acttaataga cgacatcttt gagatgacag cagcagcgtt tcacgttccg
    12661 aaaaacctac taaagggaga catgagtggg ttatcggatc aagtggacgc ttttttaatg
    12721 ttcgaaatca taccgattgc tgaacttatt caggatgcgt ttaacgctag tctctatgaa
    12781 gtagaagaat acttgtcagg gaattttgta cgtgtggata caactatgat caagattact
    12841 agcttcaaag atttggttga cgctattgat gtaggcatta gaaatggagt atttacaatc
    12901 aacgaaggaa gagaacgcgt tggaaatgat cgctctgata aggcgatggc agatgaaata
    12961 tttataacta aaaacaatca acaagtatcg aaaggaggtg aggcgaatga cgacaatgaa
    13021 aacatttcta gcagtaaaga atgaaggcgc agtaccgcaa atttttattc agggatttat
    13081 tggttctagt tggttctttg aagggaatac tgacaaggga atcaaaaata ttttggatag
    13141 tctaggtgat caagaagaaa ttgaagtagt aattaattca aacggtggag acgtatttca
    13201 agggattgct attgggaact tacttaagtc aaataaagca aaagttaacg ttgtgattaa
    13261 cggcttagcc gctagtgctg cttcaattat cgcaatggct ggcgatacta taaaaatcta
    13321 caacaatgca caattgatga ttcaccgcgc ttccacatac ggagaaggaa atgtcgatga
    13381 cttccgtacg attgctgacc aactggaatc aattgataaa tcggtaaagg cttcatataa
    13441 aacacgattc aatggcacag atgaagcatt gcaagaactt cttgaaaaag aatcgtttat
    13501 ggatgcagaa acagctttga gttatggatt ggtcgatgaa attatcgatg cagaaaatag
    13561 ctcaggtact gaagctaaaa aagaacaaag cgttgaagaa attttgaatg acgttaaaga
    13621 aaaaagagca gaaaaaattg ctgcatttac agcagcatta aataaaacat ttggacaagg
    13681 agatgtaaaa taatgacagt taaaaattta aaaggtgtaa cagctgcaag cgaccaattg
    13741 atgaaagctt ttaaagatgg taacgaagaa tcttttagcg cagctatggt aagcttatct
    13801 aaggaaattc aggataaaat tttagaagaa gcaacagcaa aaaatcaaga tcaattagta
    13861 ttaatgaacc gtggtcagcg tgtattaact acacaagaaa caaaattcta taacgaagta
    13921 gtgaaaaacg aaggttttgc aggggtcgaa gaattagtgc cagctactgt atttgaacgt
    13981 gtatttgaag atttagaaca atctcatcca ctattgcaaa aaattacttt tgttaacaca
    14041 actggtgtaa cagaatggat tgtgtcacgt ggagtcaatc cagcatggtg gggtaaactt
    14101 tgcgaagctg ttaaaaaagt tttagataat ggctttgatg taattaacat gaagcagttc
    14161 aagctatcag gttatattcc tgtatgtaag gcaatgcttg atttaggtcc agtatggtta
    14221 gatcgttatg tccgtactgt tttagtagaa tcattgagaa ttgcattaga acaagcaatt
    14281 gttgatggta ctggtaaaga tatgccagtc ggaatgatgc gtgacatgag caaacaaact
    14341 agcggagaat atgctgaaaa aaaagcagaa cctattacag ctttagatgc tgtaactatg
    14401 ggcggtttga tggcgcgact atcaaaattc aatatcgaag gtgtgaatga tccgatttat
    14461 cgtaatgtga atccttctga tgtggtccta attgtgaatc caactgatta ctggtctaaa
    14521 gtattcccag ctaagactgt actaactgct aatggagaat acgtacaagt attgccagta
    14581 ccagtttcag atttgcagtc aacggctgtg ccagaaggaa aagcagttat tggggtagcc
    14641 tcagattact tcatgggtgt aggatctaca ctaaaaattg aagcttcaga tgaataccat
    14701 tttgttgaag acgaacgcat ttatctagct aaacaatatg caaacggtca acctaaacgt
    14761 aacgatagtt tcattgtatt agatattagc gctttgggaa ctactactac aactacaaaa
    14821 ccaacaacca caacaactac aacacaagcg taggtgatca gaatgaagta tattctttgt
    14881 cagccggcaa tcaatcggtt taaatgggag cttgaagttt gtttaactaa tctgaagaaa
    14941 ctaggaatca aagatatcgt attgcttttc agcagacacg atgatcagat tcctattttt
    15001 tttgagaagg aatatggtgt tgaagttcat gtgtacgatg atctgcggga cgacaaagag
    15061 tatattcctt cgattaaacc atatttatgg tggaaatatt tagaagaaga tcattcgcgt
    15121 gaggacgacc gatatttcta tatcgattcg gatgtcattt tcaataaaag aattaatttg
    15181 cgcaaattgc cttctaaaga tgatgtttgg tattgtagcg actgctgtag ttatctaagt
    15241 cttgattata ttagaagctg tgaaaacgga gaaaatattc taaaagatat ggcaaacatt
    15301 gtaaatgtta cagtagaatc tttggaaact ataaacacta attcaggagg cgcacagtgg
    15361 gttattaacc gtcctaaagc gaattattgg aaaaaggttt atctggattc taatcggcta
    15421 tatcgctacc ttagagggca aaaaacaaat atacaaatct ggacagccga gatgtgggca
    15481 cagctttgga acatgatgta tttcaatatt ggtcctaaag ttcacgagga attagacttt
    15541 tgttttgcta ctgatccaat agaaaaagtt aaagaagtaa aaatcttaca caatgctgga
    15601 gtaacaacaa acgatgaaga tttatttttc aaagggagat acgtgacttc tacgcctttt
    15661 gatgaagatt tatcatttgt aaacaagaaa aaatgctctt acgcatatgt taaagcaatt
    15721 aaggcggtgg ttagatgacg cctgaacaag tgactgaaga attgctaata gctgtgaagg
    15781 ataatattta cgttacctgg aacgaagaag atgagtcaat taaaaagatg atagctaaaa
    15841 atgctgttta tcttcaaagt aaagtgagta caacactttc tttttctcct gaaagcttag
    15901 aatacggatt gctaatcgaa agatgtagat acgactggaa tcgtgcttta gatgagtttg
    15961 aacaaaattt cgctagtgag ttattaggtt tcattcaaca ttatgcgcta caagaatata
    16021 ttgcaggtga tgtgaatggc gaataatcgt agactcgaag aaacattcaa cgatggttgg
    16081 ttaaagattt tgacgcaaac taccaaaaga aatgaactag gaaaaaagat tggtgtagaa
    16141 gatacagaaa tcacttcttt aaaatttaga aatctttcca tgagagatag tgatataaca
    16201 gctatggatg cgatgggatc gaaattaact aagaaagtaa agactccatt tcatccaatc
    16261 gccaagaaat ttaataaaga tcaatatttt atcgtaatcg atagtatgcg ttacaacgtt
    16321 atctatgccg attacgataa tttttatatc tatttttatc ttgaaagtgt gggtgaatat
    16381 ggtgattgat aattctaaag aaaaagaacg tttaaataag caaatttctg ctatcaaaac
    16441 ttccttagaa gagcattttg gcctcaaact ctttcaagac tccgtaggcg aggatgagct
    16501 acctgatgat tttaattact tcattctcga aacaggagaa atagaaatga tcactgagcc
    16561 aaaatatagc gtgggtcaaa atctatatct aactttctat tcagaaaata gagaagattt
    16621 aacaggagac tcactagata ttatttcatt gattcaaaat cgttcgattc gttttcagag
    16681 aatggatccc aatcatttaa aactagagaa ccaagatcgc tatatcgatc aattggtatt
    16741 tacgtttaga cgattattga agagtgattg tcatggctaa aaatagttgg gagctaaaaa
    16801 taaatggaca tgatgaactt cttgtgcgga tggaacgcta ttcaagcgag agcgaacgac
    16861 tgattaacga agcattgaaa tcaaaaggtt cggctattgc agtggatagg attacagaaa
    16921 aaattcctgt ttctgaagca gatttaagaa gagggcacca acacgcaaaa aatagtcgtc
    16981 cacttaagac tcaatacatt aatttgggtt tcatcattag acctacaaga aaatttgagt
    17041 atttaaaata tcctgatttg gggataggta cttctaaaag aaatcagccg gacgaattta
    17101 tgagaagagg attaggtctt gcacttgatc caattacaga acttctgatt cgtcaattcg
    17161 ataaattaaa taaatagggg gaacaacaat ggctaaaaca aaaactgtag taacaacgtt
    17221 cgataacgta agtatcaaac gaattgcttt taattttaag aacgcaggaa atgcaatcgc
    17281 aacgaattgt aatggacaat tagatggcga aacagaaatg caaacggtgg ttaaaaaatg
    17341 tggagcgaca gaagtaaaat caaaatctaa accaatcaat atgacggtaa caattactgc
    17401 acatgtaccg atggaagttt atcgacgttt caatgggttg aaacaagatg gacgtattaa
    17461 accaggcatt tactcttacg gtcctgattc cgtaggcgaa gatttctcac ttgctgcaga
    17521 gatcgtggat gacttcgaag aaaatagaaa gttagttggt atgttagcat gcacttcgaa
    17581 tacaggatta acattctcta ttgaaaatgg tgcggatgaa gtagctgcgt tagaactaga
    17641 aacaaaagtt atgcaagatg aatttggtaa attttatcat gaagcaattg ttgcagaact
    17701 tgaagaagac ttaacagatc aatggatgac gaatctatct gctgatgtga ttaaaaaaac
    17761 ttcagttgtg acaactacgg ccactcaatc acagtaaaaa aaacggaggt agcgaaatga
    17821 acgaagatta ctcaaaaatt gaactaaacg atggaacaat tttgaattta gaacctaaac
    17881 tgaatatcaa gaaattattg atgatcaata gagattttaa cacagacgag tttgcaaaaa
    17941 tgactgtggg aaaaggatcc atggatattt ctgttattca aggtgcaaag gctgtgtata
    18001 ttgcttaccg ccaagcgaac atgactgatt atatttcatt cgatgaattt atcgataaat
    18061 gggattttga tatggctact gccagctata tttatcaatt gatgatgttc aaacaagcac
    18121 gcgatgctta tcaaaaagaa tttgaaaaag caaataagga aaaaaagctt caaaagtaaa
    18181 aattccaaag ctcttagttg aaacgtgggt cgatgtctat tcgatgttga ccgacgtttt
    18241 ttctatgcct tcagatttgg ttttaagcga tatctgttta gatgacattt tgcaaatggc
    18301 ttacaacaag agtgcttatg aaggatggaa aaactatgca ataaaccaat cccagaaaaa
    18361 ctaaagaaag gaggtaaaaa atggctaaaa agagaacaga agcagaagta actttcatag
    18421 ctaacgatga cggattgaaa tctacgttaa aagaaatcag tgctgaatta actaaaaata
    18481 gagcagaatt aaaactagaa caagctcaat tacaacagac tggttctgaa tcagacaagt
    18541 taggaagtaa attatcttct ttagaaaagc agtatgaatt acaaagtcaa aaagttgaag
    18601 taactagcca acgtttagcc aatgcgaaaa aatattatgg agaaaattcc accgaagttc
    18661 agaaacttga aagagaactg attaatcaac aaacagcgca acaacgtttg tcaaacgaaa
    18721 ttgataaaac gagtaatgca ctagctcaag caaaaggcga aatacagacg tacgagtcta
    18781 caatgcaaca gttggatagc gaacaaaaaa atgttcaagc tagtgcttct ctgattgaat
    18841 ccgaatacaa aaaatggcaa gcaactgctg gtcaatcagc ttctgaagcc gagaaattag
    18901 cgaaagccca agaatatgtt tctcaacaat ctgaaaatgc ggagaaaacg atagatatcc
    18961 taagacgaca gttagaagct acacagtctg aatttggcgc tacatccaca gaagcaatgc
    19021 agatggaagc gaagcttaat gatgctgaac gtgaatttga agagttagga caagctgcta
    19081 aaaatgtaga tacaactaac ttggacgata tcggaagcaa aatagatatg aataatttaa
    19141 tggaagcttc tgacgtttta agcgacattg gcgataagct tacagaatta gggaaacaag
    19201 cagtggactc tgctaacagt gtaggtagtt cccagagtaa gatacaagct aattttggtt
    19261 tgtctaaaca agaggctgaa gaattaacga atgtagccag agacatttat tataaaggtt
    19321 ttggagaatc gttagatcag tccacagatg cattgatttt ggtaaagcgt aatttaggag
    19381 atttaaataa tcaagattta caaaatatca cggaacaagc tatggtccta gaaaacacca
    19441 tgggcgctga tatggatgaa acgttacgtg gtgtaaatgg cttaatggtc aatttcggct
    19501 tgagtgctca agatgcaatg gatttaatgg tttcgggtac tcaaaacggt ttagataaaa
    19561 cgcacgaatt aggcgacaat atggcagaat atagccaatt atggagtcaa atgggatatt
    19621 cagctgatga aacgttcgga atgcttcaaa atggtttaga tgcgggtgct tataaccttg
    19681 ataaagtcaa tgacttagtt aaggaaatgg gaatatcgtt aacagatggt cgatttgagc
    19741 aaaacatgga tatgtttagt gaaagtacta gaaaagcttt tgaagagtgg aaaaatggcg
    19801 gaggaacaca aaaagacgtt attaattcca tgattcaaga ttttagcaat atggatggtc
    19861 aatacgacca attaaataaa gcttcgacaa tttggtctgc gcttggcgaa gataatgcga
    19921 tgaaagttgt ccaatcttta actgatgtta accatacatt tgatgatgtt agtggatctg
    19981 cacaaaaaat gaatgaagat tctactactc cgttgcaaga gttgaacggg aaaatagctg
    20041 aattaaagga ttcattagct cctataggca acacaatcat agatgcactc gaaccagtaa
    20101 ttgattttct aggaaagatg gctgatgcgt ttaataatct tccacaacca gtacaggatt
    20161 tcattgttgc tataggtggt cttacagcag catttggcat actgttaccg gcaatactag
    20221 ccgtgtcata tttatttggt ccgatgatgc ttataattgg aggaattata gctgttatag
    20281 caggcgttat tgtggcaatt aagaactggg gtgcaattac tgactggttt agtggtttat
    20341 ggaaaaaatt tactgattgg ttgggtgata cttgggaaag tataaaagac ggagcctcat
    20401 cagtttggga tggggttaaa gaaacctggt ctggatttgt agattgggtt caagatattt
    20461 ggcaaggagt ttctgattgg tttggagagc tatggagcgg attagttgaa ggagcttcca
    20521 acatctggca aggagtccaa gaaacttggc aaacattcgt tgattgggtt tcaaatattt
    20581 ggaacggagt caaagaagta tggtcgatta tttgggcaga cattgtagga attgttcaaa
    20641 taccatggac attaataacg tcattgattc aagctggtat taatattatc gtgggtattt
    20701 ttgatgtagc tggacagtta ttgggcgcag cttggcaagc tgtttggaca cctatttctg
    20761 atttccttaa aaatacttgg gatactatga cacaatggat aagtatcgct tggaatggga
    20821 ttgtaactac attccatact atatttgatc cagtagtggc atggtggaat ggtatatgga
    20881 cagctattag tactacggct tcaaatattt ggaattcaat tagtgcaaca gcttctagta
    20941 tttggaacag tatcaagaat acaatcacta gcttggtaca agcagctgct acagtaattc
    21001 aaaatatttg gtcaactgta tctagttggt taggtggaat ttggaattca atcagctcta
    21061 cagcatcaaa tatctggaat agtgtgacta gtagtataag caatgctata aacgcagcta
    21121 aaagtgccat tcaaagtgtt tggaatagta tatcttcgtg gattagcgga atttggaacg
    21181 gtatcaaaaa tactgctttg aatctttgga atggaattac aagcactatt agctctaaag
    21241 taaacgatgg aaaaaatgca atttcaagcg gttggtccaa tctaacaggt attgtttccg
    21301 acatattcaa taatgttaaa agtacaattg ctaacatatg ggaaggcatc aaaaagactg
    21361 ttagcgctcc aattgattgg attagagaca aaatcagtgg catttttgat aatttgaata
    21421 tttctatacc acatattccg ttaccggaat ttatcatgga aggcagcttt aacccgctaa
    21481 aaggtcaaat cccccatttg cgtgtaaaat ggcatgctaa aggaggtatc tttactaaac
    21541 caactttact aggtggaatg aacggtgtcg gtgaagcagg acctgaagca gttttacctt
    21601 tgaaaagatc tgttttgcaa gaaattggtg atcgtatctt gagtagcaca tcagtttcat
    21661 ctagggcaca aacgattcaa cctgtgaata actacgaatt caatttcaca attgatggta
    21721 acgcagatga ggttactatg aagcaaacaa ctcaacaaat cattgatagc attacaaaaa
    21781 ttcaaaatga taatgcttcg gcatggcgtt aaacaggaga gtatttctcc tgttttttta
    21841 gtattaaaaa ggatgtgaaa aaatgactga ttgtatacat tctataatcg atggatttcc
    21901 tgattatttg cataaattgg ctttagctga aagaccaacc ataccttctc caaaaagaca
    21961 gagagttgaa acttctgttt taggtaggtt aggtggctta gtacaagatt actcgtttga
    22021 agatatgtcg tttacattgc actataacta tttagaggat gtggaagacc atcaagcgtt
    22081 caagcaatcg ttttatatca tgcgtcattg gttaaactat gcaaagaaat tagaattctc
    22141 tgacgatccc aacgtctatt acgttatcca gactatcgat attggggatg cagaaaacga
    22201 tattgttgaa tggggagagt tcgatgtaaa tattactgcg aaaccattcg caagagttca
    22261 agaagatgta cctataaccg tagataaacc acagtcattt aacttgctga ataatagttt
    22321 agaagaaagt tttccaaaga ttatcatcac tccttcagct acttcatgcc agttcatctt
    22381 aaatgattat gtgtttagtt tcgaaggctt agtagcagga actgatgtag tcattgatag
    22441 tgatttgatg ctttgctacg aagagcaatc ggacggagat attttagatc ggtccaacaa
    22501 aatgaagacc atgcaatatc cgacattgca agtggatatc aatcatttta attgcactgg
    22561 tttgagcaaa atacaaattt atcgtaacgg gttaaggtag gtgaaataga tgatcgataa
    22621 tttaataact atttacgata aaaatgacgc gaataattta gctgaacatt tatatgatac
    22681 gcaaggttta ggcgctttgt cagactggtt aacagctact gttagcaata aactaaacgg
    22741 agccgagata tttcagggta cttattcaat aaacggaact aatgcagatt tgattgtaga
    22801 cggacgtatt attcagtgtt atgtagatga aaatcgagca aaacagcgtc tacggattta
    22861 ttatgcaaaa acttctgtaa taggcaatac gatagaagta aaagctgaac ctattttcaa
    22921 tgatataaga aaatcggtgt tgaataaata tgacagcgga acagaaaaga tcactgctac
    22981 tcaggcatgg caaaacgcaa aagttttagc gaaaccagct atcccttcgc agttttcttt
    23041 ctcgtcatta gtagatacgc ttgctaatgt gaagatagaa aaggcgaatt ttttagaatt
    23101 ctttggtgga aaagagggat ctattctaga tcgatttcat ggtgagtttc taaaagataa
    23161 taacacatta cgtcatgaaa aaagtctagg tacggatcat aaaatcaaag cgatttatac
    23221 taaaaactta actggtcttg acttagagat tgatgctcaa agtgttttag ttggagttta
    23281 tccattcatt agcagctctt cagaaggaga agacgagatc actctaccag aagaagttat
    23341 tttcacggat tacgtggatg attatcctgc tggatatgtt tcttttgttg attttaaaga
    23401 caaagcgact gatgtagcca cattaaggga agctgctaaa gactggttga aaacaaacat
    23461 agataaacaa aaaccacaag tgagtggttc gattgaatta gtaccattga ggcatcaaag
    23521 aggctatgaa aaatttgttg atctagaaaa agtttcgatg ggtgacggag tagatgtgta
    23581 tcatccacag ttaaaagtga atatgtcagc gagaattgtg gaatatacgt ttaatgttct
    23641 aaccaattca tacgataaat tagttgtagg aaacgtcaaa acaaacttct tagaaaacac
    23701 agagaataat gtcagcaatt tgattaatga tgccattgat caattgaaaa acggtggcga
    23761 aatcagtgat ttactcaatg atattgtaga tcatcaaact gatatgatta ctggtcaaaa
    23821 tggtggttat gttttattag atcctaaaga agcgcctagt cgtattttga ttatggacac
    23881 accagataag aataccgcaa ggaatgtttt acaaatcaac aatgctggta ttggtttttc
    23941 taaaactggc attaatggaa catatgaaac ggcatggacg ttagatggcg gattcaatgc
    24001 ctcgtttatt acggctggtg aaatagtagg aattactatt agaggtacta cattaattag
    24061 tgatggtgct gattatagaa caagtattgc taatggcaaa atgacttggt actcaaaaaa
    24121 agttaacaaa gatattatgg agctagaagc acgtgattat gtaagtgctg atgccggtat
    24181 tgtatcatac accatgaaaa ctggtggtgg tttcatgatt agaaatccac agggtaactt
    24241 ggtttttagt acgtgggata atggtaataa cagaccgttt ctatcttttg gtgcgcccaa
    24301 tttcaggtat agcaatgcta gttatgtaac ttctggcgac ggtagttctt taagcattaa
    24361 tggtagtgcg ggtaactcat gggaatttaa ggtagctggt aggactatga aatttactag
    24421 tgatggtatg ctaacgttac caggttgttt ttttggttca tgggaagatg ggaaacttgc
    24481 taggtttgaa caatcaacgg tacaagtata taaagatttt actgttagag gtactaaaaa
    24541 ctcaactgta ccaacagaac attatggaca acgactattg aacgcttatg aaactccaga
    24601 atattatttc gctgattatg gggaagccgt tacaggtgac aatggtaaag ttcgtgttga
    24661 tattgacccc atgtttgctg aaacagtaaa tctaagtcgg tatatgacac atgtgacacc
    24721 tacagaacta gttttgtgtg ctgttactca tgaagatatt gaccatttca tcattgaaac
    24781 tagtaagcca aacgtattag ttagatggaa tttagtggca caccgtctag ggtatgaaga
    24841 tattagatta aaagaggata cagcatatga tagcacagtg cttgaccaaa aacgttttta
    24901 aaacgaagac aaggaggtat ataaatggct agcagtttat ataatttggc tttagatttc
    24961 agcaaagaat taaactacac caaagctatt atggctcgtc aaggtgataa agggattacg
    25021 gtgacggtta aaccgtttct aaatggcttg cagatggata cgagtggcgg aacatttact
    25081 ttaaaaggaa caacaccatc taaccgttac gtagataatg ttgcaactag tgtaactagt
    25141 gaagaagtca cgttttctct tgatggcaca tttatgagtg aagcaggata ttataaacac
    25201 tgctacgtag aatatagaaa agacaatcaa attttaacaa cgcaagatat catttttttc
    25261 tcactaggag tgtctgacat ttcgcaaggc caagccgatg aatatgtttc gcaattagaa
    25321 gagttgattc gaaagtacaa cgaaactttt gatgctttta tggctgaaat caaaggtaga
    25381 gtggatagct taaatcaaca gattactgat ttaactggtc aagctaaaac gctacaagac
    25441 aagttagatg ctctgaaaga agaaatttct aagttaggta acttacaagt gatgtacagt
    25501 aacagcatcg acttcgggga ctatgattat tcggagaacc cgaatttaat gccatatatt
    25561 acagaacctt gggttggacc tttgttaggt aatggacata cagttaaaga tagtgttaaa
    25621 agggttataa ctcatactaa aactagaaca gctaattccg gcgacatatt atcgcttggc
    25681 ttaggtattc cgtgtacagc ggaagctaat aataggtatc ttataaccac tttacgacca
    25741 tctacgacat acacattaag cgttactatg agtgtcggtt cagattggac tggagagacc
    25801 aatactatag gggttaggct tagatattta aatgaacaag gtggtataga actgcccatt
    25861 aatgcattaa taccagcaaa tgtagaacgc gataaaatgg tcactcatac atttactggt
    25921 ataacaaaag ataatttaac tagcataaca aactgttatg ttgaaatatt ctcacttaat
    25981 agtgaatata aaggaacggt tagtgtcagc tatgacgtta agttaaaagc acactaccct
    26041 aacttactag atggccctta ctggttaggt aaagttcctt taggcgagaa tattgctgac
    26101 cctacagttg tctttccaca caaaactagt gaatatatgg tttatggtag acgcaataca
    26161 gaaaactata tcgctgacca aacatatacc atatcaatga aagcaactaa actaacagtt
    26221 caatcgtttg ccgtgtatat tgctgctggc cgcgttaaag taggagacat gaagcctact
    26281 gaggggttag ctaatacatg ggagttaact tttactgtaa ctaagcaaca tatagatagt
    26341 ggggtaacta attatctgga aatataccaa tacccgtcag ctacaaaagg agctgttcaa
    26401 attgagtggc tcaaactaga aaaaggcaat actcgaaccc cgaatattag tgagtataaa
    26461 tatcgtggta ctggtatgcg tgattcaaac aatccaaaag attatgtttg ggatctagca
    26521 ccagaatatg tcgaagataa tttggccaca gatattaaaa tttctgaaat tactggtaaa
    26581 gcaaacaatt ataccgatgg gaaagtatcg gagattaatt cgcagttgac tgcttcaatt
    26641 aatgaagtag acaccacagc taaggatgct caaacaaaag cgaatgctaa tgcgactgct
    26701 atagatgaat tagacaataa gatcgatgaa cgcattaatg atacagctac taccacatta
    26761 acagttacaa acgggaatac cggatcagca aagctttatc gtgaaggaaa aacagtttct
    26821 atatattttg tggctttaaa cggaaaaagc agtggtggaa atgattcaac gatactaaca
    26881 attccagaag gctatcggcc accaattagt tttgagcaac tggttggctc gatagaccgt
    26941 tctactttga acagtgctca gttatctatt ggtgcagatg gagccattaa atggcgaaga
    27001 aactcaagtt atggatcgga ttataccttt gcaattactt acacgattta gaaaagcgtg
    27061 aatcgatatg aaggcagcat atagaccaat tgaaccttac ggattcgagc aaatcattgt
    27121 gaatgatgaa gaacatttac cggaagaatg cacagaagtc gaaccaccga ttccaaattg
    27181 gaaaccgaaa ttcaattact gggagggaaa taaatgaaaa acatttggaa atatggacgt
    27241 actggcggag agtacgcagg aaaagtattg gacgacatgc ttgtatccgt tccttacaca
    27301 gatcagccac cgcttgaagg aattcgtgct gatggcgaac cgttaacaat cgctgatcaa
    27361 atgtttgatc ctaaattgaa tcaatggatt attttagcga acgcactaga tcacaacgat
    27421 ttaaacaatc tcaaagcaat gtatgagtcg ttagaaaatg agaacggcga tttaaaacag
    27481 atcaatgcca aactcatgct aagcgatgta gcaattaaac aggaaaatac tgcattgaaa
    27541 gaaaaagcgg atagtttagc acaaatcaat tcaaaaatga tgcttgcttc gttacaaaat
    27601 agcaaagaca tttcagaaat taaagagcaa ctaaatccag cttcaaaggg aggtgagtag
    27661 tatgtttagt tttagcgatg tgaaaatgat gtatgattgg ggctgtttca ctaacgaaca
    27721 agtaatggtt ttcgttccgt tgtgcattac tgaagaaaaa gcagataaaa tcattagcaa
    27781 agaagagagc gcatcttaat tgatgtgctt tttattttga ttcaaggagt tgtcacatga
    27841 ttaatttagg ggaatgggga gcgatagcag gatcaataac cgctatcgtt tctttgattt
    27901 tattagtaat aaaaccaatt actgcatctt tctcgaagat tactgagact ctttcaaaag
    27961 taagtcacaa tttagatttg ctgactaaag atttagaatc gagcaaatca gatcgattga
    28021 tgattcatga agaactaaag aaacacgatg aaagattaga tacacatgca gaaaaattgg
    28081 tagaacacac gcaacaaatt aaaactttat ttagggagag aagaaaatga ataataaaac
    28141 gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta ctttcgtggg
    28201 gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca tcattactgg
    28261 ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca aaatgttctc
    28321 ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa ttactattac tgcgatgagc
    28381 ctgttaacgg ctcttttttt attacccatt aatacgtttg cttatactat taatgacgag
    28441 tataatttag cgccgaatca aggagactcc agattagcaa ttcctaacaa aattattttg
    28501 cacgaaactg gaatagatgc accagcaaga aacgtagccg ccaacatgaa aaataattat
    28561 aacggaagca attcttatac tacagatgtt attggtgacg gtgggattgt ttaccgtgtg
    28621 ggtgagcaag gatatgtttc gtggggagct ggtaacgcta atccttatgc gcctgtacag
    28681 attgaattac agcgcacata tgataaagca ttgtttgaaa aaaactatcg agcttatatt
    28741 gaatatacaa gagatagtgc aaaaaaatat ggaattccat tgactcttga tcaaggaact
    28801 tctttattta caaaaggaat catttctcat ttgtgggtga caaattatgt ttgggggaac
    28861 cacacagatc catatggtta cttatcgcaa atgggagtta gcaaagaaaa gcttgcttat
    28921 gatttagctc atggatttac cgatgaaaat ccaacaactt ctgaaaacaa gcctgtcatt
    28981 gatccaacac gagctggtgc agctaatcct acgctgacag atggaacaaa tcacgcccac
    29041 attgatcagt ttggggaaat cgaaaacgca aacttgcatg tggctggatg gcacattgct
    29101 aactataaat acgagtatat tttcattatg gactacaata ctgggaaaga attagctcga
    29161 gtaagagctg atggaattta tagaccagat gtaaaccaag cttataatac ttctggaaat
    29221 gttggttatc atgtatcttt caatatgcgt aattttccta gtaagaaagt ctatgtaatg
    29281 atgcgggcaa cgaatgatcc agagggaaac actaaaggcg gtgcgcaaga tttccatgac
    29341 aaacgttggt atttaaatat tcctaaacga taaaaatagc tcctcgttga ggagcagtac
    29401 ataactatat tgacaactat aaaaattatt cgataaaata gtgatgttat cgcatatctt
    29461 cactatcacc cataaatagt cacactccaa gctatgcgat aacaggtttg ttgccacaca
    29521 ttctactggt tgattgttta tggctttatg tggcaacaac cagtaccctt agctcagttg
    29581 gtcagagcag acggctcata accgtccggt cgtaggttcg agtcctacag ggtacattaa
    29641 cgtagccatt tgaatcgttc tgtgttagaa tttttttgaa gagtattata caagctaaag
    29701 cttttcttca ttgccactca aatgagtggc ttttttatgt atccttttat ggattaatga
    29761 aaggatgttt cacatagtta tacttctgta tatttgaaaa gttttacttt gatttttaaa
    29821 tagaaagaca tttgggttaa attgtgagat aataataaag aagagtttaa agcgcacccc
    29881 aaaccacttc cccataagtg tgttacgctt taaactcttt tatatttgaa gccattaaaa
    29941 agcataccat atttttgaaa aaaagtgaga aaaaaggctt acaattggag tggtagttaa
    30001 ttagtgactt atttttgatt ttatagcact gatactataa aatatagata tcatcatatt
    30061 acacaatctt aatactaact taaaaaatat ctcctttcac aagtatggtg ataaaattcg
    30121 ttccgggcta cctttttagg tagcctactt taatctttat acctttctgg atcaacgaaa
    30181 gtatacttta tatagtcata acgccgatga tcgctacgtg cgtccggcac gtcagtcatg
    30241 aatcggctac actagactag acagaaaaaa taaggtgtgt agaataataa aaaacacact
    30301 ggaggatttt tcatgtcaag acgtcaacga agaacctatt caaaagaatt caaacaacaa
    30361 atcgtcgatc tctatctcgc tggtaagcct cgcgcag
//
LOCUS       DACESX010000021        30229 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.021, whole genome shotgun sequence.
ACCESSION   DACESX010000021 DACESX010000000
VERSION     DACESX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.021"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(53..1117)
                     /locus_tag="IXF88_000810"
     CDS             complement(53..1117)
                     /locus_tag="IXF88_000810"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1507555.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(1114..2199)
                     /locus_tag="IXF88_000811"
     CDS             complement(1114..2199)
                     /locus_tag="IXF88_000811"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1507556.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(2212..3189)
                     /gene="mvaD"
                     /locus_tag="IXF88_000812"
     CDS             complement(2212..3189)
                     /gene="mvaD"
                     /locus_tag="IXF88_000812"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1507557.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(3182..4126)
                     /gene="mvk"
                     /locus_tag="IXF88_000813"
     CDS             complement(3182..4126)
                     /gene="mvk"
                     /locus_tag="IXF88_000813"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1507558.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            4448..5101
                     /locus_tag="IXF88_000814"
     CDS             4448..5101
                     /locus_tag="IXF88_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1507559.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            5186..6091
                     /locus_tag="IXF88_000815"
     CDS             5186..6091
                     /locus_tag="IXF88_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1507560.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(6093..6725)
                     /locus_tag="IXF88_000816"
     CDS             complement(6093..6725)
                     /locus_tag="IXF88_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1507561.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            7045..7569
                     /gene="infC"
                     /locus_tag="IXF88_000817"
     CDS             7045..7569
                     /gene="infC"
                     /locus_tag="IXF88_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1507562.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            7641..7841
                     /gene="rpmI"
                     /locus_tag="IXF88_000818"
     CDS             7641..7841
                     /gene="rpmI"
                     /locus_tag="IXF88_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1507563.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            7894..8253
                     /gene="rplT"
                     /locus_tag="IXF88_000819"
     CDS             7894..8253
                     /gene="rplT"
                     /locus_tag="IXF88_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1507564.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            8505..9842
                     /locus_tag="IXF88_000820"
     CDS             8505..9842
                     /locus_tag="IXF88_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1507565.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            9890..12019
                     /locus_tag="IXF88_000821"
     CDS             9890..12019
                     /locus_tag="IXF88_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1507566.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            11994..12683
                     /locus_tag="IXF88_000822"
     CDS             11994..12683
                     /locus_tag="IXF88_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507567.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13091..13777)
                     /locus_tag="IXF88_000823"
     CDS             complement(13091..13777)
                     /locus_tag="IXF88_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1507568.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13913..14107)
                     /locus_tag="IXF88_000824"
     CDS             complement(13913..14107)
                     /locus_tag="IXF88_000824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507569.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(14157..14597)
                     /locus_tag="IXF88_000825"
     CDS             complement(14157..14597)
                     /locus_tag="IXF88_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1507570.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(14601..15446)
                     /locus_tag="IXF88_000826"
     CDS             complement(14601..15446)
                     /locus_tag="IXF88_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1507571.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            15595..17013
                     /gene="pepV"
                     /locus_tag="IXF88_000827"
     CDS             15595..17013
                     /gene="pepV"
                     /locus_tag="IXF88_000827"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1507572.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(17070..18017)
                     /locus_tag="IXF88_000828"
     CDS             complement(17070..18017)
                     /locus_tag="IXF88_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1507573.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(18240..18590)
                     /locus_tag="IXF88_000829"
     CDS             complement(18240..18590)
                     /locus_tag="IXF88_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1507574.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            18790..19869
                     /gene="pepA"
                     /locus_tag="IXF88_000830"
     CDS             18790..19869
                     /gene="pepA"
                     /locus_tag="IXF88_000830"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1507575.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            20013..20333
                     /locus_tag="IXF88_000831"
     CDS             20013..20333
                     /locus_tag="IXF88_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1507576.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            20355..20819
                     /locus_tag="IXF88_000832"
     CDS             20355..20819
                     /locus_tag="IXF88_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1507577.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            21024..21629
                     /locus_tag="IXF88_000833"
     CDS             21024..21629
                     /locus_tag="IXF88_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1507578.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(21668..22957)
                     /locus_tag="IXF88_000834"
     CDS             complement(21668..22957)
                     /locus_tag="IXF88_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1507579.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            23385..23864
                     /gene="rlmH"
                     /locus_tag="IXF88_000835"
     CDS             23385..23864
                     /gene="rlmH"
                     /locus_tag="IXF88_000835"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1507580.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            23949..24458
                     /locus_tag="IXF88_000836"
     CDS             23949..24458
                     /locus_tag="IXF88_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ1507581.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            24558..25208
                     /locus_tag="IXF88_000837"
     CDS             24558..25208
                     /locus_tag="IXF88_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507582.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            25659..26597
                     /locus_tag="IXF88_000838"
     CDS             25659..26597
                     /locus_tag="IXF88_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1507583.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            26610..27662
                     /locus_tag="IXF88_000839"
     CDS             26610..27662
                     /locus_tag="IXF88_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1507584.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            27928..28563
                     /locus_tag="IXF88_000840"
     CDS             27928..28563
                     /locus_tag="IXF88_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1507585.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            28754..29545
                     /gene="msrA"
                     /locus_tag="IXF88_000841"
     CDS             28754..29545
                     /gene="msrA"
                     /locus_tag="IXF88_000841"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1507586.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            30025..>30229
                     /locus_tag="IXF88_000842"
     CDS             30025..>30229
                     /locus_tag="IXF88_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ1507587.1"
                     /translation="MRSTKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQR"
BASE COUNT         9438 a         5481 c         6226 g         9084 t
ORIGIN      
        1 gcttttactt ccgccgtttg tacgtgttta gagcgtgaga caaaagtgtt ctttactttt
       61 gtcccacgct cttttctgtg cgtcttccat acttcactaa gtcgatccct cttgcttcgc
      121 accaatcctt cactggtccg gaaaagatca gagattgttg gtgtagggcc gcggtattcg
      181 ttgcgccgac catagtataa agcagacgaa gctcttcttg ccattgcttc atcagtataa
      241 tagtttcttc tacgccgtga ttcatcagat gcgtcagtac ggttcctgaa gtacccacgg
      301 catttgcgcc gagacaaagg gctttaaata tgtcataggc attgcggata cctccggagg
      361 ctaggatttc catagaagtg tctgcttcat ttgcttcaag caatgaagag actgtcgatt
      421 gcccccagtc agctaaataa ctcagttccc gcttggagcg acgggcgttt tcgatctggg
      481 tgaagcttgt tccgcttcga ccgctgatat caactgtatg aactcctaaa gcagctagat
      541 cattgagcgt ttctcttgtc atgccaaaac cgacttcttt tacgatgagg gggacgtcaa
      601 tggctgtttg agtttcttga ataagtactt tccaattggt aaaatcacgg tctccctcag
      661 gcataaccaa ctcttgaggt gcattcaaat gaatctgtaa ggcatctgcg tgaaacaatc
      721 ggatagcttc tttggctctt tcgacagaag tccctgcacc gatgttggca atgattttcc
      781 cgtgaggata ttcttgtcgc ataatcgtat aagtatcggc taaagaaggg actttcaagg
      841 cagcgctgac tgaaccagtg gcaatcatca aatctgcttc tcttgcgacg attgcaagat
      901 cttggttgat tttttttgtt ttttcgcttc cgccagtcat cgcattgata taaaatgggc
      961 tgcttagtgt aaacccaact gcctgtgtac ttagatcgac atcagaaact gctatttgcg
     1021 gcaaagggtt atggatgatc cgtacgaagt caaattcgtt tttttgttta tcatgaaagg
     1081 ctttagctag tgaaacatgt tcgtcttttc gattcatcgg ttttcctttc tttgatcgct
     1141 atagacatgt aacggcagtg gagtgatttc tgctttttcc catgcactca ttaaaggaag
     1201 aatgccagat ttctggtcaa caattacgat tccgcaatcg cccccacctg caccagaaga
     1261 ttttgcggct ccttcatact gttcagctaa attacacaat ttgttcaaag caggcgtttc
     1321 gatgacgacc ccagtgattg cagataaatc atgcagtaat tgtcggtttt ttcgaatcat
     1381 ctgttggatc aacgttacat tattttcttt aaaccctttg atcatttcgt tgacacattc
     1441 tgtactattt tttaagaaaa gctgataagc caccatttta tcttctctcg aacggtgaac
     1501 ttgatcgacc aaatcagaag tagaggcagg gctacccgtc caaccaatca ataaacgtaa
     1561 atcttcagga gcaatcaatg gctcaatgga tagacctggc caatccaggg caagtaactc
     1621 actgatagaa tgctgagttt cttgttcttg gagccaagga tggtcgaagg ttgagaaagc
     1681 gatccagcca ccataacagc tagcagcgat gtcgccgcag gaaccattat cttgaactgc
     1741 taaattggct agtgctgcaa tcttgaaaat ctccaactga gataaattca acgcgtaaaa
     1801 aacattcaag gctttgaccg ttgcaacggt cacggcaccg ctagaaccca atccatattt
     1861 ccggccattt gaactgtcta actcacttgt tactttcaga tcataaaagg ataaaagaat
     1921 gtttttttct tgtgcatact tttcagtcaa gcgaatagca gcaaggatat aatgaaaagg
     1981 attttcccga atatctaaaa ctaattctcc gttgcgtctt gtccaacgta caggcatccc
     2041 actatattgt gcagattgga tacttccgac ttttcgtgca gattctacag tgactgtcac
     2101 gaattgatcg actgcagcga taactgctgg atggcctgtt tcaacaactg catattctcc
     2161 ggcaatatag agtttgcctg gtgcagatac ttcaatcata aaaatctccc tttattcaat
     2221 aatcgcaatt cctggaccag catgtgcagt aattacttgt tctttgctaa ataatttggt
     2281 aaatgtttct tgtacctcag ataaatggct gttttccact aaaactttga cattagggcc
     2341 agcatccatc gtaaagtagc atggaatgcc ttgctttctc aattgtcgca ccgcatccat
     2401 cgcttttaag ctgtcaggag accagtaagt gaatggaggc tgggcagcta aagtcgttcc
     2461 atgcattttt agtccgtttc tttccatcgt ttctccgaga agttggaaat cttttgtttt
     2521 gattgcttgt ttcaattgat atagatcccc ttcaacggaa tctaaccagc cttgataaaa
     2581 actggatgtt tcgactgtcc gacgcatccc atttctgctg gacacttctt ttttctggtc
     2641 gttgatcaaa acgaaaacca ttgctaaatc gtcttcgaaa gaatcggaag ggactggctt
     2701 agcgtaagaa cttaagtcgt catggccttt ttcccattcg acgaaaccac cgaaaatact
     2761 tcggcaagct gaaccagacc cgcgtcgagc aaatcttgaa agagagagat catctaatcc
     2821 aagcttaaga gcagtattgc aagcgcctgc taaagcagct agcccgctgg cagaggaggc
     2881 taatccagct gcggtaggaa cgaaattctg actgattact gaagctttta gtgagaggcc
     2941 ggcttcttta cgaaaaaggt caagaaactg actgactttt tttgttgctt tttcgtcttg
     3001 caaggtgcca tctagataaa attcatctac catatagcta tcagaaaaga tgacttctgt
     3061 ctctgtataa aatgcatcca atgttaacga aaggctgttg ttcattggaa ggatgagttc
     3121 ttcatttttc ttaccccaat atttgattag agcaatgttg gtatatgcgc gtgctttgcc
     3181 tttaaacata ggtatgtact cctaatcctt gtatccaagt ttcagcagca cccgcatctt
     3241 caagtgcttg actgatttct tgggcttttg tttttgtttg tgctaatgca atcatacagc
     3301 caccgcgtcc accgccagtt aatttggcac ccaatgcccc ggtatctttt gctgtttgga
     3361 tcaatagatt aagaaaatca ttgctgattg tcagcttttc cagaagtgat tgtgattcat
     3421 ccattgtctg tgccaacgtt tctggtgaat tttcaatgat tgcttgtttt gcttgcttcg
     3481 tcaagtatcc taatttttgg atcatttgtc cagtttcatg aggctgggtt tcaaagaggt
     3541 gagcgacatc tttgacggct tctcttgttt gtcctttgat tcctgtatca gcgacaatca
     3601 aaaaagcatc gatgttcaaa gaaaagtagt cgaaaggatg cccttttgta aaataaatcg
     3661 gctgcaagct gctagtagcg gctgcatcga ttccactagg attaccgtgg gcgatttttt
     3721 ccgaaagctg gacattttct aatagtattt cacgagacaa aggaaatgct aagtaatcat
     3781 aaaaagcacg agtgactgct gtggcgactg cagcgcttga tcccattcca cgttcagcag
     3841 gaatcgtgct ttcaatggtc agttgcaagt ttttttggat gtcatgctgt ttttttaagt
     3901 gaatgaataa ttttttgata ttttttaatg catgaggagc ttcggctaaa tttcctgaat
     3961 agtaggaaga aaccagttga tcgtccattg catccagctc ttcaaggaat gcggtgactt
     4021 ttgttgcata gaaaggaaag gcaatcgctg gttctccata aacaaccgcg tgctcgccca
     4081 tcaatatgat ctttccgctt gattctcctt ggccatagtt tgccataatc taactccttc
     4141 tcacgtaaaa gtgattttta agcaaaaaat aaagcgccca aaacgagttg cgctgcttca
     4201 cgaatttttt tgtccactaa gggtctcaaa aaacattcct cctttatttt actaaaacca
     4261 tctcagaagt aaaactaaga atacgttttc taaagaaaaa acactttttt cttaagaatt
     4321 ttttgacttt ttttcttctt tgactagaaa aaaatacaat tgacactact agatattggg
     4381 ggtatattta aaaaggttgg agttaaacac aatatttagt gatgaagtga gctggaggta
     4441 aatgagcgtg atcgttttag caggtacaat tggtgccgga aaatcaagtt taacagaaat
     4501 gatggctgaa cactttgata gtcaggcatt ttatgaatcg attgatgata atgaagtctt
     4561 gcccttattt tatgcaaacc ctgaacagta tgcttttttg ctgcaaattt attttttgaa
     4621 taaacgtttt gcaagcatca aacaagcaat gaaagatgac aataatgtat tggaccgttc
     4681 gatttatgaa gattcactgc ttttccatct aaatgcagat ctaggaagag caacagaaac
     4741 agaagttcgt gtgtatgatg aactgctgga aaacatgatg gaagaacttc cctatgctgc
     4801 ccataaaaaa catccggatt tattagttca tattcgtgta tcttttgata cgatgttgga
     4861 gcgtatcgaa aaacgtggtc gttcgtatga acaattgtcc tttgatccaa gtctatacga
     4921 ttattacaaa gaattgaaca gacgttacga tcaatggtat gaagaataca aagaaagtcc
     4981 aaaaatccaa atcgatggag accgctataa ttttgtggaa gatcctcaag caaaagaaga
     5041 agtcttgaaa atgatcgaag aaaaattagc agagattcgc caaaccaaag tagccatttg
     5101 atttacagtt gctcaaataa agggtaaacc tttatttgag cgactttttt ctttatactg
     5161 gaaacaaatg tcaggaggat agagtatgcg ggaaaaacac tattttaatc aagaccgtaa
     5221 tttttggatc aatgtacagc aaaacgatgt gctagctgtt aaaaacttaa aagaagaata
     5281 tggcattagt gacgagatgc tgacttattc tctagataaa aatgaacggg cgcgcgtaga
     5341 gtacgattct tttgatgaag cgttactgct tgtatcaaat gttccgcacc agcaaaaagt
     5401 cgataatcat tatgaaacta gtcctatcgc ttttatttta aaagaagacg gtttgtttac
     5461 atttaccacg ccgaataccg agtacgtgat tcgactaatt cgatcgcttt tggaacgaat
     5521 gccagacatg agtgtataca gtttattgtt tcgtacattg tttttgattt ctgattcctt
     5581 ttttccttta atcgaggaag tgaatagtga acgtcagcgg cttaatctga aattaagaga
     5641 aaaaacaacg aacaagaatc tgctgcaact ttcagattta gagatcggtt tggtttactt
     5701 agttacagga acaaaacaaa atgtagtact tttagaacaa atcaaggcat tggctattta
     5761 tcgcaagttg tcagaaaagg aaaaagaaga gttggacgat gcgctgatcg aagcacgaca
     5821 agcagttgaa atgacgaact tagcttcgca gattttagac cagctctctg gcacttataa
     5881 taatttgctg aataacaact tgaatgacac gatgaaattt ttgacggtct ggtcgctgat
     5941 cttgaccgtt ccaactattg tgacaggatt cttcggaatg aatctccagt taccctttac
     6001 ccattccgtt ttcggatggg gaatcgcctt gatcatcagt ttggtcttat ctatttggat
     6061 gcttattgca ctgtggcggc ggattcgttg atctagtctt tgatctgcaa accaagggct
     6121 tgggcgatca caagtgcttg ctcttggttg acgattagtc ccgccaaaga atcttgagat
     6181 aaagcgattg ctgtgaagaa attagtcgta aagtctaatc ccttcagaga agttcgaaac
     6241 cagttcgaac cggtcaactg attgtcagac aaaaaaaggt ttttccaagt gacctcataa
     6301 aattcgctgt cttctaattg acaatgatcg aaggagacag ctttcaagtt tgtactacta
     6361 aaggaagcca tcgaagcaat acaatcagtg aaagtacagt ctcgaagata actttcggca
     6421 aaattcgtac ctgttaattt acattgatga aacacgactt ggtggaaact gccaccaatc
     6481 cactccagat tggaaaaatc acatttttca aaaatgacat tactgcattc aaaatgacgt
     6541 aaaactgttt tttgcatcgt aagtttttgg atatgactct ctcttaatac catatttttg
     6601 gcatcgagat agctttggtc acccccgttg atcagaagac cagagaagat ctcctcatct
     6661 tctaaataaa actctccctt tgtaagaaga ggaagagaag gtggaatagg ttcgattttt
     6721 ttcatgtggg ttcacctcgt ttttctttag tatagcaaag aagcgggaat cactgctaga
     6781 aagtctgcta atctaatcat tttcagaaaa ggaagacaaa ctgtatgaaa aacattgaca
     6841 aaaagattag tttttgctat actatgatgg ttgagaaata aaagcagaag cacccgcttc
     6901 tcgccttagt tgataatgtc actgggctag atagttttca ctggttctat taggtgaagt
     6961 acgcgcgggt tctgtaagag aactcgtttt tttattgccg tttattcggt aagcttttct
     7021 ttctctcata atatttggag gtgaatgacc atagcaaagg atatgatggt taacgacggc
     7081 attcgtgcac gagagttacg attgatcggt tcagatggtg agcaattagg agttaagaca
     7141 aaagcagaag cattgaatat tgcagaacaa gcaaacttgg atcttgtgtt agttgcccct
     7201 ggtgcgaaac caccagttgc gcgaatcatg gactacggta aataccgttt cgaacaacaa
     7261 aagaaagacc gcgaagcgcg taaaaaacaa aaagtgatca atgtaaaaga agttcgctta
     7321 agtcctacga tcgacttgaa tgacttcaat acaaaacttc gtaatgcacg taagttcttg
     7381 gaaaagggcg ataaagtgaa agcttctatc cgtttcaaag gccgtgccat tacccataaa
     7441 gaaattggtc aaaaagtctt gaatcgctta gctgaagaaa ctgcggatat tgcaacagtg
     7501 gaacaaaaag cgaaaatgga cggacgcagc atgttcttga cgctggcacc gaaaaacgac
     7561 agtaagtaat ttgaatgact aactggctgt tggacagccg atagtcggat aacgaaagat
     7621 tttgaggagg aaatatagtc atgccaaaac aaaaaacaca ccgcggatca gcaaaacgtt
     7681 tcaaacgtac tggtaaaggc ggattgaaac gcttccgcgc gtttacaagt caccgtttcc
     7741 acggcaaaac aaaaaaacaa cgccgtcaat tgcgtaaagc aagcatggtt tctagtggcg
     7801 acttcaaacg tattcgccaa caactagcaa aaatgaaata agaagtacaa ataacagaat
     7861 ctgaagattt atttttatta ggaggaatta tacatggcac gtgttaaagg tggaacagta
     7921 actcgtaaac gtcgtaaaag agtgctaaaa ttagcaaaag gttactacgg ttcaaaacac
     7981 actttattta aaacagcaaa agaacaagta atgaattcat acaactacgc atatcgcgat
     8041 cgtcgtcaaa agaaacgcga cttccgtaaa ttgtggattg ctcgtatcaa cgcagcagct
     8101 cgtatgaatg gcttgagcta ctcaaaatta atgcacggtt tgaaagttgc tgaaatcgac
     8161 atcaaccgta aaatgttagc tgacttggct gttaacgatg cagcagcatt tactgcattg
     8221 gctgaccaag caaaagaagc tttggctaaa taagctaaac aattataaag aggaaagaag
     8281 ataacgaaaa gttatgttct ttcctctttt tttatttaca atcctccatc aaatgaaaca
     8341 ggacaataac gtataagtct aaaatagcat aattaaggtt ttttaattga aaactgaaca
     8401 tttatggtca ccctttaatg aaagcgctaa ctacataagt tagaatgagt ttgactgata
     8461 tattagtttg cttatcagaa aaacaaaatg gacggaggaa taacatgagt gatattatta
     8521 ttggtatttt gttagttatc tcattctttt tcatggtctg gtactgtgta aaaggctaca
     8581 acttgatggt cggctttgcc atcatggcta ctgtatggat gggattagcg ctagtcggaa
     8641 acacgttttc accaaacccg gcgatggaag ggcaaggagt aattgatgtg ttgacgcata
     8701 tctatacgac ggggccagct gagtacgcca aatcgatctt ggtaaatgtc ttctttggtg
     8761 cctttttcgg gcgtgttcta gtagatagcg gaatcgcagc taccctgatt cgaaaggtag
     8821 ttgaacttgg aggggacaag ccaagaatca cgatgagctt actatgtatt gtaaccgctg
     8881 ttatattcat gtcgatgaca ggaatcggac cagtgatttc tattgcagtt attgtgttgc
     8941 caatcttgat gtcattaggt atttcagtac cggttgcact gttctcattt atgggctcga
     9001 ttatggctgg aatctttgct aatatcgtca atttcaaaca atatcaaacg atttatgctg
     9061 gtttcaatcc agcggctgaa agctatactt ataatgacta tttccaaatt ggtatgattg
     9121 ggatgattgt aagcttagtt gtggtactaa cggtcgctaa tatatcaatg aacaaaaaga
     9181 aacgctatgc aatggcagca aacgtaccag ctgaaggtgg cgacgcacca atgatctcat
     9241 ggttagccgt attattacca gttttagggg tcgttctttt agatcttcca atcatcttag
     9301 gttttattct agcaggtatc tgggcattgc tgtttactgg aaaattgcgc ggaggttata
     9361 aagaaatctg tcgtcagttt gcgaaattat ttacagatgg cgcagtagat gttgcaccaa
     9421 tggttggttt cttgatgacc ttagcgatgt tcaataattc agctgcgtat gcttctcctt
     9481 atttttcagc tatttttgga gattggatac cgcaatcacc attagtcttg gcaatcgtat
     9541 ttgctatatt gacaccacta ggatttttcc gcggtccaat gaatctagtc ggttcaggct
     9601 cagctatttt agctgtcgta ttagctgtta atccaacgat gtcgccagcc tttttgttcc
     9661 cgttatttgc aatcacaacg attgctcctc agcatttaga tattacacaa tcttgggttg
     9721 cttggggatt aggttataca aaagtaactt ctcgagaata tatgaagaaa tccatcccaa
     9781 ctggttggat catcggagcg atcttatgcc tgatcacgtt cttattgtac gggaacgcct
     9841 agtaaacaaa acgtcgataa aaacgattag atgaatggag gaaaagaaaa tgaaacgttt
     9901 tgtgcgaatg ggaattgatg ttggcggtac acatacgaaa gcagtcgcaa tcgataatgc
     9961 gacgcacgaa atcatcggaa aatcttcagt gaaaacaacc cacgatgatg tccgtggagt
    10021 agccgcaggt gtcgttcagt cttttcaaaa ttgtttgcgg gaaaataata tcagtccaga
    10081 agatgttgtg tttgttgcac attctaccac acaagcgact aatgcactga ttgaaggaga
    10141 cgtagcaaaa gtcggcgtaa tcgggatggc aaaaggtgga ttagaaggtt ttttagcaaa
    10201 acgacaaact agattgaacg atatcgattt agggaataag aaaaaaatag agattgtcaa
    10261 tgcattctta cctgtcaaac acttaaatgt agaccgtgtt tcagaaacga tttcttcttt
    10321 agaaagagaa agagcggagg tcttggtttc ttctatggca tttggagtag ataatgggga
    10381 accagaacga gttgtttatg aagcagcatc ggtaaaatcg atccctacaa cgatggcttc
    10441 tgacatcaca aaattatatg gattgacaag aagaactcgg acagcagcaa tcaatgcatc
    10501 tattttacca aaaatgctag atacggcaac ctctacagaa gattccgtac gagaagcagg
    10561 ggtcaatgtt tctttgatga ttatgcgcgg agacggcgga gtaatggaaa tcaatgaaat
    10621 gaaaaaacga cctgttttaa ccatgctttc cggtccagca gcatcagtca tgggctcttt
    10681 gatgtacttg agagcgtcta atggtgttta ttttgaagta ggcggaacaa caaccaatat
    10741 cggggtcatt aaaaatggac gtccagcaat cgattattca attgtaggtg gccacccaac
    10801 atatatctct tcattagatg ttcgggttct aggtgtggca ggcggttcga tggtacgggc
    10861 taatcagtca ggaatcatcg atgtcggtcc acgttctgca catattgccg gtctagatta
    10921 tgcagtattt acggagacag aaaaaatcaa aggtcctaag gttgaatttt tctctcctaa
    10981 agaaggagac cctgctgatt acgtcaaggt agtgatggaa gacggagaag aagtgactat
    11041 tacaaataca tgtgcagcca atgtccttgg attagtgcaa gaagaacatt tttcttatgg
    11101 aaatgtccca tctgcacgaa aagccatcca agcattagct gattattgtc atacgacggt
    11161 tgaagacatc gcagaacaga tcatggaaaa atcctatgct aagattgaac cggtgatttt
    11221 agaattggca gacaagtatc acttggagaa agatcagatt tctcttgtag gtgtaggggg
    11281 cggcgctgcc tcattgatta cttactttag taataaaatg ggtgtgaaat attcgattcc
    11341 tgaaaacgcc gaagtgattt cttctattgg tgtagcactt gcaatggtcc gagatgttgt
    11401 agaacgtatc atcccttcgc caagtaaaga agatatacga tcgttgaaaa atgaagcgat
    11461 gaataaagcg attgaatcag gcgctacacc agaatcgatc gaagtccacg tagaaatcga
    11521 tccacagact tcaaaagtaa cggctatcgc tactggttct acggaagtga aagcaaccga
    11581 tctaaccaag gagatcacaa cagaagaagc actagagctt gctgcagagg atatgcgatt
    11641 aaataaaaat gaagtttgct tattggaaaa tacacctttc ttttatgtat gcggcgaaca
    11701 gaatcgttca aaaaatgctg gaagtcttcg gatcatcgat caaaaaggat ttatcaaggt
    11761 ccaacgtgga catgcttcat gtatgaaaac aactgcagcc aattatatga cagctgtcga
    11821 acaactttgg gaagatatgg cagtttatca aactgaattg attgcccgac cagaatttta
    11881 tctctgctta ggagcaagag tctctgattt tacagcgacg gatctggaac aactacagct
    11941 tttgatggat ctggaagtat ctactatgga accggaggaa gaagtgatcg tcgttgccgg
    12001 aaatatcaaa caaacataag ttagaagagt tactaaacga attacaacag atccccgaag
    12061 aaatttgggg attctaccag tttcggcgag atttattttg gaaaaagatt cctttgtcca
    12121 agcagaaaat tcttatacaa caatcaatcg attgtggtat cgaaacggca tgtagcatca
    12181 aaaaaaagta cccttttgcc gatgtaggag aaatttgtga acaaatggct gttccaatcg
    12241 tttcctgtga atcagagcaa ataaacgaac gaatcacttt tgcaacttat gcagaagatg
    12301 aagggattcg attgatgacg gagccgttag aaaaattaaa atgttcagga ctagcttcga
    12361 tctctaaaga aacagcgcaa gctttgatta ttggacatga actatttcac cacatcgaag
    12421 cttctgttaa gggaatttat acgcaaagcg aaaaaattgt gctgtggcgg ttgccttttt
    12481 atacacatca atcaaacatt cgtgcattga gcgagatagc agcgatgagt ttttcaaaag
    12541 agatgaatca aagcgatttt tcgccttatg tattagaggc agtcttactt tggccatata
    12601 atgaaattcg tagccaaggg atcttagaag aaataaaaaa aatagaaaaa agatgtacgg
    12661 agtatgattt agattacaaa taaagagact gtgacaaaag tcgtgtcaca gtcctttttt
    12721 tccgaataaa cgatgttctc ctgtcaactc ctcggtcaca agtcaaaata ttcaacaaac
    12781 atgagaagct gttttttgaa attgttcctt gtgactcgga gatgaacggc aggctcacga
    12841 cctcttggaa aacggtgttc aaaagcttgc atctgcggta ataagtccag ccaagttaaa
    12901 aacataagaa gctattttta cttggctgtt cttattactt gatgcagggc agctttttca
    12961 caacctctca ataaacggtg tgcaatcagc tgaccctcgg tattagctca aaaaaaacgg
    13021 caaaatatga ggaactattt tgccgtttct ttctctaata ctcaggtcaa aacgctgatt
    13081 ccaaaacctc ttatttgatg ggtctgtcct gaatatcttc aaaataatcg gggtaattat
    13141 gaatcacgta ttcttgctcc tcgataatca tatgtaaatg catcgtgcaa agaaatccga
    13201 cttcatcaaa gttatgatca atcattgatt gaagcattgt ctgatgttca tctaatactc
    13261 gaccccagtc taattctgaa atcgtcaaac gtaaccatct gaaacggttc aagtgcgtac
    13321 tataactttc tagccaatcc caaatttctc gttttccagc aatttcataa caagttcggt
    13381 gaaaatcagt atccaaatca aaaaaagctt ttttattttg agcgattact gcctgatcgg
    13441 tttgttctag tattttttct agtttagctt gatcttgctt ggtcattttc gcactgcatt
    13501 cttgcaggat cggtttttcc aacttttccc gcgtataacg ggctaatgaa gcagatcgga
    13561 tatcgatttt tgaaacataa gtgccggact gtggaatggt ttttacaagc tcttgattgc
    13621 gcagttggat cagtgcttct cgaatcggtg tacgcccgat agctagttgt tcttctaaca
    13681 ctttatcgga gattttactt ccaggtgcta actcggaata aatgatttgt ttacgtatag
    13741 attcataagc ttgctgttgc aaattattga taaccattta accttccttt ctatgcagat
    13801 ataatctatt ataacataaa tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa
    13861 aaaaagccct gataggcatg cctattagag cctcgaaaag aaaaatattg aatcaatctg
    13921 gtcgtacctc ttgtatcggt ttgacacgac cgccgattct tttagcaaaa gcttcggatt
    13981 cttttttgtc tttgaaaatg agcagattgt ctaagttttt ttcccgagtg cgacagccag
    14041 ttttatcgat gtaaccgtgc attactttaa cgacatacat ggggatcacc tgcttttttc
    14101 ttttcattat acgaactact cgacacaata caagaaaaat gcttagacgg tctttattaa
    14161 gaatttacat catccaaaga agcgtacgct tggaataact gatctaactc cggattgact
    14221 ttgctcagct tcactaatcg ttgcgaatca gaaagtaaaa aacagtgttt actactgcca
    14281 gtagttttta attttttgtc ttcagttccc acaatttcgt agctaaaatc caaccgtgtc
    14341 cccgtatact tttggatagt tggatggatg ctcacttttt ctccgtaatg gatcatttct
    14401 ttatagctgc aatttacttc cagaacgggg atgatgatcc cagattcttc gattttttta
    14461 tagggaaacc ctagatgtac caataaggct acccgagcct cttcaaacca gcggatataa
    14521 ttgctgtgat gaataatccc catttgatct gtttcataat aaaaaggttc tcgcacataa
    14581 cctgtaaatt tctccatgac tcatgcctcg ttttccattg attttttaca aaaataattc
    14641 atccatttcg gaagtgcttc gctgatttgt gtcggcaaaa caatatattg ttcttttgcc
    14701 agctggtctc caatgaaact atgaagataa atagctgctg caatcgtttg gtcattttga
    14761 ggaaactggg ctaaaaatcc tgtgatcatc ccagctaaag tgtcacccat gccgcccgtt
    14821 gccatagcag cagaacctaa aggattggtg tagaagtgct ggtgaccata aatttttgtc
    14881 tcatgacttt tcaaaacgag cacagcaccg atttcctgtt gcttcttttt gttttcttta
    14941 tcccgttgtt cttgtatcgg tagagaactc aaccgttgcc actccatttg atgaggtgta
    15001 agtacagttt gttcgggata gggtagcaaa agatgattag cagcaagtaa acttaaagca
    15061 gagccatcaa tcaccagcca ttgttctttt gattggtttt ctagtacttg tttgaaacgt
    15121 tccaaactct tagaagaagt acccaatccg ggaccaatca agagtacatc tgcttgcttt
    15181 attacggaaa gaaactggtc agtatccgtc caaactacaa tcattgcttc tggcagatga
    15241 ctgtgaagag cactccgatt tttttcatca gttactacag tcgtgagacc cgctccgctg
    15301 tgtacggcag ctaaagcact cataatgatt gctccgccat attgttcatt gccgccaatc
    15361 aaaacgatac gtccaaatgt tcctttatgt gaaatttttg gacgaggtgt gatgactctt
    15421 ttcactagtt gttcgtttat cagcatctaa aaacctcctt ttactcatta tagcgtatat
    15481 tttttttaga gaaaaacaga taagaaaagg aattgtcaga ttttttgaac tatttttttc
    15541 tgtaatttgt ggtatcatga acacgaagac ttaaataagt ggaggattaa ctttatgaca
    15601 attgattggc aaaaagaagt agaagcacgt aaagaagact tgcttgaaga cttgaaaaat
    15661 cttttacgtg tcaacagcga gcgcgatgac tcaaaagtaa caccagatgc accatttgga
    15721 cctggacctc gcgacgcatt gaaacatatg ttagcttatg gcgaacgtga tggatttaaa
    15781 gtaaaaaatg tcgataacta tgcagggcat attgatcttg gcgaaggaga cgaaacgcta
    15841 gggatatttg gacacatgga tgtcgttcct gcaggagatg gctgggacac agatccttat
    15901 gaaccagtaa tcaaggatgg aaagatcttt gcacgcggat caagcgatga caaaggtcca
    15961 agtatggcag cttactatgc gatgaagatc attaaagaac ttgacttgaa actatcaaaa
    16021 aaagttcgct tcgtagtcgg cagcgatgaa gaaagcggct ggggcgatat ggcttattat
    16081 ttcgaacatg aagaagaacc agattttggt ttctcaccag atgctgaatt cccaatcatc
    16141 aatggtgaaa aaggaaatgt ttctttagct ttacgcttca aaggcgacaa tgcaggagat
    16201 tatgtcttga aatcttttgt ttccggactt cgcgaaaata tggtgccagg aacagcaact
    16261 gcagcacttc aagtaccaag tgcagatgca gctatcgcga tggaagaagc attttaccaa
    16321 ttcatcgaag caaatccagt aagcggaacg attgaagcgg acaatacgta tgtaaaaatc
    16381 gaattagttg gtaaaggcgc acatggtgca agcccacaat ctggtatcaa tgcaggttct
    16441 ttcttggcat tgttcttaga taactatgaa ttcctaggat ctgctaaaca atttatccat
    16501 gtagcagctg cttatgtcca tgaagatttt tatggagaaa aactaggagt cgcttatgaa
    16561 gatgaaaaaa tgggcaaatt gacaatgaat gctggactat ttgcttttga agaaaatggt
    16621 acagaggaag cgaacttcat caatatgaat ttccgttttc caaaaggtgt aacagtagac
    16681 ggattgcaaa gtgacatcga acaaacagtg gggcaagaag gcgcaacagt tactcgaggt
    16741 gctcgcgtaa tggaaccaca ttacgtacca atggatgatc cattggtggc tacattattg
    16801 caagtctatg aagaccacac tggagaaaaa ggctacgaac aaatcatcgg tggcggaact
    16861 tacggacgtt tgttgaaacg tggcgttgct tatggagcga tgttcccagg atatactgat
    16921 acaatgcatc aagcaaacga atttatgagt cttgatgact tgttcagagc aacagcaatc
    16981 tatgcagatg ccatctatcg tttggcgaaa taattaatta aaaagaggat caagaattgc
    17041 aaattatttt tgcagtcttg atcctttttt tactttctac tgtggtattc atgataaaca
    17101 tgatatagat tcaattcata ttctttctta gcgttcgtga cgactgtatc caaaatcaac
    17161 cctgttacta atgaaagaag tgctaaagtc atcaatccag tacttaaaat cgcagaaggg
    17221 atgcgtgtaa taaagcttgt cagcatatat tcgcgaatca caggaatccc cgtgaccaat
    17281 ccaaataaaa agaagaagat ggtccaaatc ccaaagaaaa gcaaaggctt ataatcttta
    17341 aacattttaa taatcatcat gatcactttg aatccgtcag agaatgtatt caattttgat
    17401 tcgctgcctt caggacgatc acgataatcg attgggattt caacgaactt gaatttttta
    17461 tctaaagcat ggatcgtcaa ttccgtttcg atttgaaaac cagaactcat aataggaaaa
    17521 cttttgacaa agatacgatt gaatcctcga tagccggtca tgacatcaag aatttttgtt
    17581 ttatacattc tagtaattgt atttctaaca aggttatttc caaaatcgtg gaaaggtctc
    17641 ttattctcat caaagtaggt tccatttgat aggcgatcac caatgaccat atctgcttca
    17701 ccagaacgta atttctcgag caaaccatgt acagcttctg caggataagt atcatcgcca
    17761 tctaccatta aataatagtc ggcatcgata tcaaagaaca tttggcggat cacatttcct
    17821 tttccttgac gaggttcttt ttttacgatt gctccacctt gaacggccaa ttcgtatgtt
    17881 cgatcggtcg aattattatc atagacataa atatctgctt ctggtaattc tcgtttaaag
    17941 tctgcaatga ctgttgaaat tgttgcttcc tcattgtaac aaggaatcag aacagcaatt
    18001 ttttcttgtt gttccatttt tcactctcct taactataga aaacaaaagc taaattaaaa
    18061 atacctcata atcaaaggaa atgctagttt ttcccgctaa atatcattta tttaaaaaga
    18121 aaatactgga atatatgttt ggaaagctct atctaaaaaa ccacccacta caaaagaagg
    18181 acatgatacc tctcttttga atagtgggtg cgtattcgtt ctctcacttt tttgacgtat
    18241 taatctgttg cacctttttt tcttgaaatt tctaatacgg ttcctgtatg agcatctgct
    18301 aaaaattcat aataaacagt ttcgccatct tctattcgag aaagacctcc ccgataagca
    18361 ctcgaccgaa cggcgaattt cctaataggt tccttttcaa aagagatcca agaaccttcg
    18421 attgcacctt cttttagaaa agatgacttg atattttcaa gtacttggtc tgctgacaat
    18481 acttttttgc gatgatacca aatcgttgaa gctaccccgc tcaataatcc tagaccgatt
    18541 ccaactgcta acccgccttt aaaatagctt agttcgcttt cttcatacat gtggacatac
    18601 ctccttcttc tcacttgtta ctccttgtgc ttattttacc acactctcat attttctttg
    18661 aaataaataa ccgctaccag aaaaaataag ctataataaa tacgatcggt ctgcagaaaa
    18721 aatcatattt tatccgcaga tcaatataag tctgagagga gtttagtctg aaatttagac
    18781 gaatagcata tggaagaaaa aacatttcaa cgaatcaaag agttaacaga attacaagga
    18841 acaagcggtt ttgaacaaga tgttcgcgct tatatggaaa gtcatatgga accgctggta
    18901 gatgagttac agttagatgg tttgggcggc atttttggtc tgcgccatca tgaagaagcg
    18961 gatgcacccc gtgtaatggt cgcagcacat atggatgaag tcggattcat gctgacacaa
    19021 atccaagaaa acgggctttt taaagtcgta cctctaggcg gctggaatcc ttacgttgtt
    19081 tctgctcagc gttttacact taaaacctct actggtaaga attatccatg tatttcttct
    19141 tccgttcccc cacatttatt gcgaggaaca agcggacaaa aatcagtaga agtaaccgat
    19201 attttgttcg atgctgggtt tgaatcaaga gaagaggcaa tgagttacgg tgtccttcca
    19261 ggagacacaa tcgttcctta cgctgaaacg atcaaaacag ccaacggaaa gaatattatc
    19321 agtaaatcat gggacaaccg ctatggctgt acaatggtat tagaagcttt agaagcgttg
    19381 caaaatgaaa cattaggtca tacattgatt gcaggcgcga atgtccagga agaagtaggc
    19441 ttgcgtggtt ctaaagcttc tgtcaataaa ttcaagccag atctgttctt tgcagtagac
    19501 tgctctgcag cagatgatac agtgacgaaa aatggcacat tcggacattt aggagaaggt
    19561 acattgatgc gtatccaaga tccaggtttg atcatgcttc ctcgtttgcg cgagtacttg
    19621 ctggatatcg ctgaaacaaa caatatccct tatcaatact ttgtttcaaa aggcggtaca
    19681 gatgcaggtg ctgctcatac acaaaacgaa ggaatcccaa gtacagtgat tggcgtagtg
    19741 ggacgttata tccatacgca tcaaacgatg ttcagtattc gcgattttga agctgcacga
    19801 gaaatgttga ttcaaacact gaaaggtttg gataaatcaa cagtgaatac gatcgtatac
    19861 ggaaaataat gcgatagtga aactataaaa tctggcaaaa gattgctttt tgccagattt
    19921 tttttataaa aagaggatca atgtttgtta gttcgaaaga aaggtgtata atttaattga
    19981 aaaatacata aacagaaagt agaggacaaa gaatgattat cccaaatagt ttagaagaat
    20041 tagcaggcta cgtagaaact ggaaagagtg tatttttctt tactgccggc tggtgcggag
    20101 actgccgttt tatcaaaccg caaatgcctg aaattgaaaa tgacttttct gattggcaat
    20161 ttattgaagt agaccgtgac aagtacatag atgtagccgc tgaatggaac atttttggta
    20221 tcccaagttt tgtcgtgatc caagacggaa aagaattagg tcgtctagtg aataaagatc
    20281 gtaaaacaaa acaggaaatc gaatcgttcc tagaaagtgt aacaaaaggc tgacatctgt
    20341 taggaggggt caagatgaat caagaatata agaatatttt agtaggtatc gacggtagtg
    20401 aacaagcact tgctgctttc aaaaaagcgg tagaagtggc tcgccgtaat aatgggaaag
    20461 tatatgtagc aaacgtaatc gatcagcaat tttataactt tatgggctat gcacctattg
    20521 atcaaaatct agtcgatcag caaactgaaa cagccaagga aatgatcgaa gaatgtaaag
    20581 aatatgggaa atctgttgat tacacaaata tagaaggaat tattgcatat ggttctgttc
    20641 gtgaatccat ggcagtcaaa ttgccaaaaa aatatgagat cgatctaatc atggttgggc
    20701 agtcaggtct aaatgcagtc gaacgcttta tgacaggaag tgtagccagc tacgtgatac
    20761 gtcattcacc ttgtgatgtc ttgatcgttt ctgacgaaga agaacagcca agtaattaag
    20821 caagtggaac ttgtacgacc tttttaatca attaactaaa acagtaaagg gactgtgaca
    20881 aaagtcttgt cacagtccct tgttacgaat aaatggtgtt ccagaagagg cttcttcgga
    20941 aataaagcat gaaaaaaatt tcatgcagtg aatgcaattt tttgttaaga taaatctcag
    21001 caaaggataa tggaggaaag aacatgattt ttgcttataa caaagcacac gtcggagaca
    21061 ccttgatggt tattatagcc gacgataaag gaacagaaaa cactgttgaa cgcaaatgga
    21121 atgttgctcg gatcaaagat gaacaaggtc aagttgttgc ttggaacttt ttccatatct
    21181 ccgatcatct aacaatcgaa gggaacggcc aagtaagctt aacagatgag gaattagcga
    21241 aattgaatcg attgatcaaa gaagcaggat tcgaagaaca gcttgaagct gatcatgaac
    21301 cgaagatcgt tgtcgggtac gtaaaaacat gcgtcccaca tcctgattct gaccatttat
    21361 cgattactga gacagaagta gacaatggta cagtattgca aatcgtttgc ggtgcaccca
    21421 atatcgaagc tggacaaaaa gttgtcgttg caaaaccagg agctatgatg ccagatggaa
    21481 tgatgatctg gccaggaact ttgcgagggg tagatagcta tggaatgatc tgttccgctc
    21541 gtgaattgca tttgccaaat gctccagcaa aaaaaggaat cttagtattg cctgaggatg
    21601 ccgtagtcgg agaagcattt ccaaaataaa gaggttgtga caagtctttg tcacaacctc
    21661 ttttttatta attttcacta ttttgatttt gttttaatgc cgattgatcg atcgtcaatt
    21721 caacagtagt tgttttttct ttcgagcctt catagtaagt gattttgaca gaatctccca
    21781 cttttttctt atacaaagca gattgaagtt ctacaccaga agaaacttct ttgtcatcga
    21841 tcttagtaat gacgtcgtat tgtttcaatc cagctttttc agccggtgta gctgtttgga
    21901 catttgtcac gatgacaccg ttagttacag atgaaggaat cttcaagatt tgttcttgtt
    21961 gctgagtaga aacggcagat aagtcgacca ttgtgatacc tagagctgga cgtgtcactt
    22021 ttccgtcttt ttccagttga ttgatgatat tcactacatc gttacttgga atagcaaatc
    22081 ccattccttc tacgcttaca ttagaagtag attcagaagt acttgcgatc ttacttgagt
    22141 tgataccgat aacttgtcct tcgatattaa ctaaaggacc accagaattt ccagggttga
    22201 ttgctgcatc tgtttggatc gcattgatat tgactgtttc gttcgattca ttcgtacttg
    22261 tcacttgtcg gttcaaggaa gaaatgattc ctgaagtgac agaatttgca tattcagatc
    22321 ctagtgggga accaatcgca atcgcaggct caccaacctt cagagcactt gagtcaccaa
    22381 aagaggcaac tgtttcgact ttgtctgaat tgatcttcaa caccgctaaa tctgaataag
    22441 catctgttcc aactaattca gcttttacct tcgtaccgtc tttcatcaag acttcaagac
    22501 cttgctggcc atctactacg tgattattcg tgactacata agcagtattc ccgtcttttt
    22561 tgtagatcac gccgcttcct tcactataag cttccaaatt actatcatct gagctgctac
    22621 tttcttcttg ctgcccgaat agtccgccaa aaccgcttga ttggttttga ctttgtaagt
    22681 tgataacaga aacgacagcg tcttgaactt tatctaccgc tttggtgata tcagaatcta
    22741 cattgacttt gacattttcc acaactgttt caccggcaga attctgattg ccgcttgtag
    22801 cagtagaact attgttgcca gtgcccatcg ctgcataaaa aatgccagct gtcagtagtc
    22861 ctccaacgat ccctccaaca agccccagac ctaacttacg ccagaggcta ttatttttac
    22921 tttttttcat ctttggtgtc acattttttc tatccatgtg ttcctcctcc aattacattc
    22981 atttatctac tgttagtata gtcttatttg ttgactttag tctactttga gactgtgaac
    23041 tttttatgaa aaactttagg aaaaatcatt gttttcagat gaaaatattt gattttcttt
    23101 ttccacagat actgtgtaaa actacgcgga taggattgga aaaataaggg ataaaacata
    23161 tccacagcct gtgtataaag tggataaatt cttataaaac gtatgtttgc ctaatgaatg
    23221 tgatgaaata aggtaaaata acactgtttt ctgtggataa acctgtggat gttgtgcata
    23281 actgttgggg aaatcttttt aacaaaaata gccaaaataa acatatagac gaaaaaattg
    23341 attataataa acaaagtgag catgaagacc aaaggagaga aaacatgaat atcaaaatta
    23401 tctcagtagg aaaattaaaa gaaaaatacc tgatccaagg tatcaatgag tatgtcaaac
    23461 ggctgaatgc ctatgcaaaa atcgaattga tcgaagttcc tgacgaaaaa gccccggaga
    23521 atttaagcga agcgcaaatg agacaagtaa aagaaaaaga aggggaacgg atcttagcga
    23581 aaatcaaaga acaagaatac gtctatgcct tagcaattga agggaaaaat cctacaagtg
    23641 aatcttttgc taaacaaatc gaccaattag gtatccaagg aaaaagccac cttgtttttg
    23701 ttatcggcgg ctctttagga ctcagcgaag ccgtcatgaa aagaagcaat gctcagatct
    23761 cttttggaaa aatgacgtat ccacatcaat tgatgcgtct gatccttgtc gagcaaatct
    23821 accgtgcctt ccggatcaat gcgggggcgc cttatcataa gtgatgcggt ttttatcatt
    23881 gatcctaaaa atcttagcaa gacaaaaagt acgtaagaaa gtaaagacta aatacttcgg
    23941 aggaacaaat gaataacaat gataggttat tgcgattgcg ttatgcgatt gatttgaaag
    24001 atacggattt gattaaagct tttgagctag gcggtgtacc actgactaaa gaagaagctc
    24061 aggcggtact gaccaaagta caggataaga acaaagataa tgcagaaaat aatgtgtatg
    24121 aaaaaaccat caacaaccaa gtttttgacg cattcctaaa tggcctgatt ctcttggcgc
    24181 gtggtccgca aaaagataag ccgatagtag acacgccgca aaaaagcaag gaaatcaaat
    24241 atatcaacaa tgtgttgcta aaaaaattaa aaatcgcctt gtctatgacc actgatgata
    24301 ttttagacgt ttttgctgag gcagaaatct atccatcaaa aggcgaaatc ggggcatttt
    24361 tgagaaaaga aggccaacga aattttaagc cttgcggaga taaatatatg cgtaattttc
    24421 tcaaggggtt aggaatttat aatcggagaa aagtataaag aaatttcatg agaaatcaaa
    24481 acaatggttg ttttgatgag agctagtttt tgtatgagat taaaatatta tcttaatcga
    24541 ggagctggat tggttttatg aatgatgaat ataagaatga tgaagataag atgctttttg
    24601 aagagataga gaatcgatgt aggctcaatt ttgagcttcg gggaaagatg tctttaatac
    24661 aacaaaaaaa gtatttagcg aataaatcag agtttactct gggacatgtt gaaaaattaa
    24721 tctcggattg gattagtagt agaagcgaat ttacaaaaat aaaacagccg attaaatttg
    24781 acatgaaaaa acttttgtta aataagagtg agataggtaa tagagatcaa tatattagag
    24841 ctaaagggca agaaataatt gattcattag gtgaaatgag gtcatacaat tatttgtatg
    24901 taacacatag agctgatgga atggtcatta ctgtggggaa atcttcttct aatgatattt
    24961 ttttagatgg agatcttttt tatcaactca atacaaatca cttgtctggc actgaaaata
    25021 taattttaag gacggaatat ggcaatgaaa tatttgcaaa atatgatgag attttaaaaa
    25081 attatttgga ctgggcttgg ataattccgg ttgagtcagg agatgctaag aaactggaga
    25141 gattattggg cgatgaacta attaataaaa aagtaccaat tttaaattat tattcccata
    25201 gacaataaaa ggctattttt catgataata ctagttataa ctgtaaaaat ggaagaaaat
    25261 ctgggaaaga agtttttaaa taagtgtatt gttcgatttt aataaataat aagcaatacc
    25321 atccgtctgt gttgcgcaac atcgtactac aatgaatatt ttttcacaaa aagaaaatca
    25381 tccatcgaat gaatggaagg ttttagtata cacgaggtta atgccccctc ttttataatc
    25441 agaggagtga ggttttttct atgaatgttt ctattttctt attgctagtc tagtttctct
    25501 tacgatgaaa ttgggattct attccagttt ttattgattt gctgataaaa taacttgaat
    25561 ggtggatata aacatgataa ataagagaag aataacaaaa caaggttgtg aaatgattct
    25621 caaatgaata taatagaaag aaaaaggagg aagaatcaat gaaaatagca attgcaggcg
    25681 ctggcgcaat gggaagtcgc tttgggttaa tgcttcacca aagtgggaac gaagtattat
    25741 taatcgatgg ctgggcagaa catgtccaac aaatcaaaga gcatggatta caagcaaatt
    25801 ttaatggtaa agaggtagaa gcaaaactac caatcgttct tcaatccgaa gtagaaaaag
    25861 aagatcaagt tgatctgatt attctattta ccaaagcgat gcagctggaa aaaatgctgc
    25921 aggatatcca atcattaatc aaaaaagata cagaggtctt atgtctatta aatggtatcg
    25981 ggcatgaaga tattattgag aaatttgtac caatggaaaa tatctatatt ggaaatacca
    26041 tgtggacggc aggtcttgaa ggtcctggtc aggtcaaatt atttggaagt ggttcagtag
    26101 aattacaaaa tttaggtgat ggaaaagaag cagctgcaaa aaaattagca gacaaattgt
    26161 ctgaatcagg attaaatgct catttttctg acaatattca ctattctatt tatcgcaaag
    26221 catgtgtgaa cggaacaatg aacggactat gcacaatctt agacgttaat atggctgagc
    26281 taggaaaaac atcgactgct cataaaatgg tggcgacgat tgtcaatgag tttgccaaag
    26341 tagcagcagt agagaagatt gaactagatg tcccagaagt cattgcacat tgtgaatctt
    26401 gttttgaccc ggaaacaatt gggttacatt atccttcaat gtatcaagac ttgattaaga
    26461 accatcgatt aacagaaatc gattatatca atggcgcaat ttctagaaaa gggaagaaat
    26521 atggtgttgc gacgccttat tgtgatttct tgactgagct tgtccatgca aaagaagata
    26581 gtttgaacgt aaaataaagg agagattgaa tggaaacttc acaaaaaata tcaccgaaaa
    26641 tttttctgaa taaagtcctc gctggtacag caacaggaat cattattgga ttgattccta
    26701 atgcagtatt aggtgccatt ttaaaatatt ttagtgcgta tccctttgcg gtaacgatcg
    26761 cacagttagc agttattttt caactagcaa cacctttgat cattggtggt ttgattgcct
    26821 tacaatttgg atttgatccg atgagaatga tggttgtagc cggagcttct tttgttggtt
    26881 ctggtgtagt caaattcaca cctgatttgg gtgaaacggg tgtttacgtt ggtgctggta
    26941 caggcgatat tattaatacg atgctgacag catcgattgc tgtcgggctg attttactga
    27001 ttggaaatag ttttgggtca gtgtcgattg tgttaacacc gattgttgta ggtgtaggtg
    27061 caggtttact tggtttttat ttattccctt ttgtaacagc aattacttct gcaattggat
    27121 cattgattaa taacttcact accttacaac cgctgttaat gtctatcttg attggttgtt
    27181 cctttgcctt tttgattatc tctcctattt caacagtagc aatcggaatg gcaatccaac
    27241 tagatggtgt ttctgctggt gcggctgcga tgggcgtagc tgcaaccact tttgttttag
    27301 tagtgaactc ttggaaggtt aataagtcag gtgtgacaat tgcgattgct ttaggcgcta
    27361 tgaagatgat gatgcctaat ctatttagaa aacctgtgat tcttattcct tgtttagtta
    27421 gtgcagtgat tacagctatt cctgtcgctc tattttctat ttcaggtacg cctgcttctg
    27481 ctggtttcgg cgtggtcgga cttgttggtc cgttggcttc tttagatgca gggctaaatg
    27541 gctttcttgt tgttctttgt tggttagtca tccctgtttt tgcggcacta attactcaat
    27601 ttttatgcga gaaagtattt aaactgtatg atcgagaaga agtcttcaaa tttttaggct
    27661 agtagatttt actaagaaat tttcaatcaa tggatgcgta agtgagatgt caacaacatc
    27721 tgacttacgt ttttttgcct ttttgagtag gagattgatg ctgacaactg aactcttcag
    27781 tgccattcga ttttttaaaa tgtagaagaa actcaccttt atgttattga ttgttcaaaa
    27841 ggcaactaga ataaaaatat gtgttaaaat gagaaaaaat ttgattgttg gatgtatcga
    27901 ataatcacta aaaagtagga gaagaaaatg atttttttgc aaaaatatgg tttttatttt
    27961 ttattattag gtgtgattag tgatttatcg acaccgtata tccttggcct tttttatccc
    28021 aaactgaatc aaatgactac cgtaattagt gtgtttggtg acgtagatag tccagtcaga
    28081 cgtgctttct tggtctggtc agttgtatct ggactttttt ttgttctttc gttgcctgcg
    28141 ttgtatcacc tatttattgg aacatcaaaa acacttgcaa tacttgctgt tgcaacagtt
    28201 ggtttatatg ggataggtga ttgtattttt actgggttat tcagtatcaa tacgaatgaa
    28261 agctcttgga atctatcgac ttggatccat aatataggtt ctgggctagg ctatgcaggc
    28321 tttctacttt tcccattgtt acttgttctg ctttatcgtc aaagtggtca aggtacgctt
    28381 agccattttt atcttgtttt aacagttatt agtttactaa ttgcaggtct ttacggatta
    28441 gcacggattc cgtcgattag tcagtttgcc ttctttaagc aattgggctt ctttcaacgc
    28501 ttaagttttt tctttaacta tttgccgatg atttgtttaa gtttttttca actacgaaag
    28561 tagaaaaagc agtgtgaagc tgattaagta aaagaatttc tctatgggta accattcgtg
    28621 tggatgtgtt cgactaagaa tagagatgag gagagcgaaa agcaagttag tcttaacttg
    28681 ttatgcgttg tgctaaattt caggtagtca ttttttgaat gaataagaag aaaacttgaa
    28741 agagggtgac ttcatgttag atgtggagca gatggaaata gacctttata atattatttt
    28801 aaatcgaaat acaagagaat gggaaagaac acaatttgaa gaggcaaaag aagacctagc
    28861 aaaaatgtcg ataaagcagg tgatagaaaa actagaggtc aacttgcgtc cgctagcact
    28921 acgagataat ttgacaccag atgcgatgga tttttattta agaatcacag gggagtcagc
    28981 gagtgcaaag gcttatgatt atgccaaaca taagataact tcacccttat accaagaacg
    29041 ggcgattttt gccggtggct gtttctggtg tatggttgag ccttttgaaa caaaaccagg
    29101 tgtaatctca gtgttatctg gttatacagg tggagagacc aaaaaaccaa attacgatca
    29161 agtcagtggt ggatatactg ggcatgttga agctgttgag atcatatttg atacacgtca
    29221 aataacctat gaagagctgt tggagattta ttggcaaatt tcagacccta cagatgcctt
    29281 tggtcaattt caagatcggg ggaaacaata tcgttcaatt ctatttgttg ttaatgaaaa
    29341 gcaaaggcaa ttggctgaaa aatcaagaga acaactgatt caatctaacc gatttagccg
    29401 tcctatcgtt acagagattc gccaagcaaa gactttttgg ccagctgaaa atcatcatca
    29461 acaattttat cagaagcaac ctaaaagata taaaaagatc aagcattcgc atcagcttca
    29521 tttatttttt cagcgacata gatagaggaa ggttgtggcg caaagcgttc agctccgaca
    29581 aaataatgag caactgacaa aaatagcttt tcatattttt ggacagttat gaattatttc
    29641 gaggagttgc tttgagaaca ccgaggaaaa ggttgtggcg caaagcattc agcttcaagt
    29701 cgcaaggaaa caggaggtat gacaagtctt ttgtcacaac tcctgttttt ttactagcct
    29761 ttatttccat gtgaaagaga attcgaattg tagaatcttg atttttctat ggaattttgt
    29821 agagaatcat cttgataatt aatagacacc taatcgaatt ccacattaat gatgaactaa
    29881 attccgtata aaagcgcaaa caataactat aaaacccgtg gtgctagtta attttttcct
    29941 agaggaaaat gtctaaacat gttggtaaat caacaaaaaa acgcatatag tagtctcaaa
    30001 actcccaaga aatgaggact acatatgcgt tcaacaaaat atactgaaca ttatacagat
    30061 acttttataa cattcaagga aattatgaga actgtttcta acatatacca caattgtgta
    30121 ccagataaga ttaaaaaccg aagaaatact gatcaactga agcaacgcga tacagtgatt
    30181 attgcttgtg ttatatgggg cataattaat ggctatacta gccaaagag
//
LOCUS       DACESX010000022        29114 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.022, whole genome shotgun sequence.
ACCESSION   DACESX010000022 DACESX010000000
VERSION     DACESX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.022"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..1034)
                     /locus_tag="IXF88_000843"
     CDS             complement(<1..1034)
                     /locus_tag="IXF88_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1507588.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLRDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFT"
     gene            complement(1116..1805)
                     /locus_tag="IXF88_000844"
     CDS             complement(1116..1805)
                     /locus_tag="IXF88_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1507589.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1911..2279)
                     /gene="rplL"
                     /locus_tag="IXF88_000845"
     CDS             complement(1911..2279)
                     /gene="rplL"
                     /locus_tag="IXF88_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1507590.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2332..2835)
                     /locus_tag="IXF88_000846"
     CDS             complement(2332..2835)
                     /locus_tag="IXF88_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1507591.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3245..3934)
                     /gene="rplA"
                     /locus_tag="IXF88_000847"
     CDS             complement(3245..3934)
                     /gene="rplA"
                     /locus_tag="IXF88_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1507592.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4038..4460)
                     /gene="rplK"
                     /locus_tag="IXF88_000848"
     CDS             complement(4038..4460)
                     /gene="rplK"
                     /locus_tag="IXF88_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1507593.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4803..5471
                     /gene="sdaAB"
                     /locus_tag="IXF88_000849"
     CDS             4803..5471
                     /gene="sdaAB"
                     /locus_tag="IXF88_000849"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1507594.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5484..6356
                     /gene="sdaAA"
                     /locus_tag="IXF88_000850"
     CDS             5484..6356
                     /gene="sdaAA"
                     /locus_tag="IXF88_000850"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1507595.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6447..7466)
                     /locus_tag="IXF88_000851"
     CDS             complement(6447..7466)
                     /locus_tag="IXF88_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1507596.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7639..8526)
                     /locus_tag="IXF88_000852"
     CDS             complement(7639..8526)
                     /locus_tag="IXF88_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1507597.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8603..9148)
                     /gene="nusG"
                     /locus_tag="IXF88_000853"
     CDS             complement(8603..9148)
                     /gene="nusG"
                     /locus_tag="IXF88_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1507598.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9249..9419)
                     /gene="secE"
                     /locus_tag="IXF88_000854"
     CDS             complement(9249..9419)
                     /gene="secE"
                     /locus_tag="IXF88_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1507599.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9443..9595)
                     /gene="rpmG"
                     /locus_tag="IXF88_000855"
     CDS             complement(9443..9595)
                     /gene="rpmG"
                     /locus_tag="IXF88_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1507600.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9757..10401
                     /locus_tag="IXF88_000856"
     CDS             9757..10401
                     /locus_tag="IXF88_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1507601.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10457..10960)
                     /locus_tag="IXF88_000857"
     CDS             complement(10457..10960)
                     /locus_tag="IXF88_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1507602.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10973..11019)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11557..12522
                     /locus_tag="IXF88_000858"
     CDS             11557..12522
                     /locus_tag="IXF88_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1507603.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12621..13793)
                     /locus_tag="IXF88_000859"
     CDS             complement(12621..13793)
                     /locus_tag="IXF88_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1507604.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13780..14925)
                     /locus_tag="IXF88_000860"
     CDS             complement(13780..14925)
                     /locus_tag="IXF88_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1507605.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14939..15871)
                     /locus_tag="IXF88_000861"
     CDS             complement(14939..15871)
                     /locus_tag="IXF88_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507606.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15988..17067
                     /locus_tag="IXF88_000862"
     CDS             15988..17067
                     /locus_tag="IXF88_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1507607.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17064..17693
                     /locus_tag="IXF88_000863"
     CDS             17064..17693
                     /locus_tag="IXF88_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1507608.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17803..18030)
                     /locus_tag="IXF88_000864"
     CDS             complement(17803..18030)
                     /locus_tag="IXF88_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1507609.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18133..20721)
                     /locus_tag="IXF88_000865"
     CDS             complement(18133..20721)
                     /locus_tag="IXF88_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1507610.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20741..21400)
                     /locus_tag="IXF88_000866"
     CDS             complement(20741..21400)
                     /locus_tag="IXF88_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1507611.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21449..21952)
                     /gene="trmL"
                     /locus_tag="IXF88_000867"
     CDS             complement(21449..21952)
                     /gene="trmL"
                     /locus_tag="IXF88_000867"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1507612.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22173..23039)
                     /locus_tag="IXF88_000868"
     CDS             complement(22173..23039)
                     /locus_tag="IXF88_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1507613.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23148..23789
                     /locus_tag="IXF88_000869"
     CDS             23148..23789
                     /locus_tag="IXF88_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1507614.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23851..25212)
                     /gene="mgtE"
                     /locus_tag="IXF88_000870"
     CDS             complement(23851..25212)
                     /gene="mgtE"
                     /locus_tag="IXF88_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1507615.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPLKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25230..26129)
                     /locus_tag="IXF88_000871"
     CDS             complement(25230..26129)
                     /locus_tag="IXF88_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1507616.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26131..26928)
                     /locus_tag="IXF88_000872"
     CDS             complement(26131..26928)
                     /locus_tag="IXF88_000872"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1507617.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26906..27610)
                     /locus_tag="IXF88_000873"
     CDS             complement(26906..27610)
                     /locus_tag="IXF88_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1507618.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27741..28316
                     /locus_tag="IXF88_000874"
     CDS             27741..28316
                     /locus_tag="IXF88_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1507619.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28434..29111
                     /locus_tag="IXF88_000875"
     CDS             28434..29111
                     /locus_tag="IXF88_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1507620.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8568 a         6167 c         5234 g         9145 t
ORIGIN      
        1 gtaaatacag tattatttcg gttgcttgct gcttcaccta gcccttgtac tcgtgtttga
       61 ttggcgactt gaccaaattg attcccgacg tctaataaaa taatagctaa aagcaaaatg
      121 gccaaatggc ttccgccaac caataaagca ataaagctta ttgtttgcat aacaagtccc
      181 atatacacga tttttctttc agaataagaa tctgccagtc gtcccacgat aggtgcagca
      241 actgctccag acaaaccaaa aatcgctaat atccctgctt cgaatgttcc ccaattgtag
      301 gcgggactgc tgatataaaa aatcaacgta gaccaaaaga tcgaaaatgt tccaaacatg
      361 aaaaatccac tgactgccgc ttctcttaat agacgttgag aagcaactaa atgcggcaaa
      421 cttttaagtg aatcaaggta tgaaacagct gtactacttt gctggacaga acgcggcatt
      481 ttccagtaaa gcagaagtgt aagaattccg actgcacata aagcaaataa atagatcatc
      541 cgccaagaaa aaagactggc aacaatacca gataccgtcc gagaaagcaa aatccccacg
      601 agtaaaccgc taagcaatgt acccatgacc ttccctcttg cttttggtcc cgctagtacc
      661 gcaccgtaag gaatgatgat ttgcggcacg atcgaaagta atcctattaa aaaagaagcg
      721 catgcaaata acgtaaacga aggcgcaaaa aaagcggcca acagagagac ggcagataac
      781 gctaccattc gaatgatcaa ttttggacga tccaccacgt cccttaaagg aactaaaaac
      841 agtaacccta acgcatagcc taattgggtc aacatcgtca atatcccgac tgcacttgtg
      901 ctaacagata aactagctgc tacttttgtt ccaatcggct ggatgtaata catattggca
      961 acaacaacgc cacaagtgat tgataatagt aaaataagcg aagttgttaa aggtgttttt
     1021 tcattactct tcattgattt cccccatttc ctatcttagt ttacaactta tggaaaagat
     1081 tggaaataga aaaatacaac tattcgccac acttattatt tgtgcttttg ttttccttgt
     1141 tttttcttgc gatctttata aatgaagaca actaacccta ctagcacagt aacacttgca
     1201 accaacgtga ctgcagtttt gttatatccg tgaaacagag cttctccacc acacgcatac
     1261 aacacagacg aagggatgac tccaatcacg atcgacaata tgatttgctg tagaggcaat
     1321 ttcatcgcat ctgcagcaaa attgaccata gaagatggaa taaccggtat catatagcct
     1381 aacattacac ctatttttgg atgtttcatt tgtcggattg cttgcaccca atgattcgtt
     1441 tcggttttct tatctaaaaa ttttaagcga tgcataagga aaattgcagt gatgttacca
     1501 agagcattac ccaaaatgtt gatgatcgca cctgtcagtg gtccaaagct caaaccagca
     1561 atgactccaa ctactgaagt aggaatacct ggaacagcac acatcaccgc tgtcaatacg
     1621 atcaagagta tcgatgcaaa agtcccatgc gaacgaatat gtgcgagtag ctctgcctta
     1681 ctcgctctcg gatttaaaaa taattggatg tctgtctgat attctaaata caaccgatag
     1741 cccactacca gtatcagaat gattcccaac gagatcaaca tcaatcggat tattttcttt
     1801 ttcatatttc ctcctgaaaa aaatctatat tcattaaata atagtttaac ataaaaacaa
     1861 aaaagcaccg aaatccaaca taggatctca gtgcttttaa atagtaaaaa ttatttaaca
     1921 gttacagatg cgccaacttc ttcaagttta gctttcaatt cttcagcttc ttctttagat
     1981 acgccttctt taactggtgc aggagcgcca tcaactacag ctttagcttc tttcaagcct
     2041 aaaccagttg cttcacgaac tgctttgata actttaactt tttggtctcc agctgcagtt
     2101 aattctacag tgaattctgt ttgttcttca gcagcagcag cgccaccagc agcagctaca
     2161 gctacaggag cagcagcaga tacgccaaat tcttcttcaa tagctttaac taggtcgtta
     2221 agttctagga tagttgcttc ttttaactca gcaacgatgt tttcaatgtt caatgccatt
     2281 ataatttcct ccgtttaatt gttctttttt atttaatgct ttatgcggca attaagctgc
     2341 atctccatct tctttgtctg ccactgcttt gacagcgtaa gccacgttgc ggactggcgc
     2401 ttgtagtaca gatagcaaca tagatagaag tccttcgcga tttggcaatt ttgccaatgc
     2461 tgtgatttct tccaatgaag agactttacc ttcgatgata ccgcctttga tttctaatgc
     2521 ttttgcttct ttagcaaatt cgtccatgat tttcgctggt gcgactacat cttcgttact
     2581 gaatgcaaca gctgtaggtc cagtgaatac ttcgtccatg ccttcaaggc cagctttttt
     2641 agctgcacgt gaaagaatag aatttttgat aactttcatt tctacgttag catcacgaag
     2701 ttgtttacgt aaacgtgtta cttcatcaac tgttaatccg cggtagtcaa cgactactac
     2761 tgatgcagct gctgtaaatt tttcgcttac ttcatcaacg attgctgctt tttttgcgat
     2821 aattgcttca ctcatttttg agtttcacct cctgaatttt gatggaagag ttttttgcac
     2881 tgaaaaggca agtcactttt atagttactt tgctcaacca gtattattca tcacctattc
     2941 acttcgttca tagtgattct actcgctttg ctcgaaagga aactcctttc gctttgctca
     3001 accagtattg ttcatcatct cttcacttcg ttcatagtga ttcacaataa aaaactctat
     3061 gccaccgcag acatagaggg actattgaat atattcaata aacgtcctcg gtaggaaatt
     3121 aaggcttgat gccacctact gtcttcggta caagttaatt attaacctca actagcttag
     3181 catgctaaac tagttgagtc aagaattatt tttcagttgg atcaatcaac attctaatat
     3241 ttaattagaa agaagcttgg tcaacatgga taccaggtcc aaatgttgtt gtgatcgtta
     3301 catttttcat gtattgacct ttagctgctg atggtttagc ttttagcaat acatcatgga
     3361 ttgctgcaaa gttttcaact aatttttcat cgtcaaatga aacttttccg attggtacat
     3421 ggatatttcc agctttgtcc acacggtaag ttactttacc agctttcact tcgtttactg
     3481 cttttgttac gtccattgta actgtaccag ttttagggtt tggcattaag ccttttggtc
     3541 ctaatactcg tcctagacga cctacagtag ccatcatatc aggtgttgca acaactacat
     3601 caaagtcaaa ccatccgttt tggatttttt gaaccatgtc gtcgtcacct acgaaatctg
     3661 caccagctgc ttcagcttct tttgctttgt ctcctttagc aaaaactaaa acagtttgtg
     3721 ttttaccagt tccgtttggt aatacaacag caccacggat ttgttggtct gcttttttag
     3781 ggtctacgtt caaacggtat gcaacttcaa ctgttgcatc aaattttgca atgtttgttt
     3841 cttttgctaa agctactgct tcggcaacag gataaacttt acttgaatca acttttttta
     3901 atgcatcttg catttttttg ctctttttag ccattttggt tcctccttga ttgtggttat
     3961 aacggttgta cctcccacgt gttctatatc tttcaatata gagccaactg agaagctgct
     4021 gtcttagggt gtatagatta ttctacagtg atccccatgc ttcgtgcagt tccttcaacc
     4081 atgcgcattg ctgcttcaac gttagctgcg tttaggtctt ccatttttaa ttcagcgatt
     4141 tctcttactt gatcgctaga aactttcgca actttatttt tgtttggttc gcctgaacct
     4201 ttttcgattt tagctgcttt tttcaataga acagcagctg gcggtgtttt tgtaacgaat
     4261 gtgaatgaac ggtcttcgta tacagaaata acaaccggaa tgatcaaacc tgcttgatca
     4321 gctgtacgag cgttgaattc ttttgtgaat cccatgatat tgatacccgc ttgacctagt
     4381 gcaggaccta ctggtggagc tggagttgct ttacctgcag gaatttgcaa tttaacgatt
     4441 ttttctactt tctttgccac gagacatacc tccttaagtc cgtgatgtgg ttaattggag
     4501 atgcatattt ctcctcccac gttttatctg atctgaaaaa atccagacaa ataatgtgca
     4561 aaacaatgca cactggtaca gtatagcaaa tttatttgtt ttttcaagat ttttttctgc
     4621 tatttttaca ttttgcttga ataaacgcta aatttattct aaaaaaacaa caatttttcg
     4681 ttctcatttc ttcttttatg ttacacttgc gctagcatgg ttcaacagat gaaagcaaac
     4741 aataacacgg ttttttagac ccttgtacaa agattcctac ataacaaaat gaggtggaga
     4801 aaatgaaaga tttaaggtat agaagtgtct ttgatattat tggacctgtg atgattggtc
     4861 caagtagctc tcacactgcc ggagctgcta gaatcgggaa aatcatacgt agtatatttg
     4921 gcgaacagcc tgattctgta gatatttatc tatatgaatc atttgcaaaa acctatcggg
     4981 ggcacggaac agatattgca ttagttggcg gcctgttagg gatggaaccc gatgatgaac
     5041 gtttagctga ttctttgaaa ttggcatatg aagccggtat ggaagtcttg tttgttccta
     5101 agagtgaaaa agccgaacat ccaaactctg taaagatgct tctaaaaaaa ggaacacgaa
     5161 agctttcagt cactggcatt tcgatcggcg gtggcaatat ccagatttct gaattgaatg
     5221 gattcaaaat ttctttaagc atgggtactc ctaccttgat cgttgtccac caagatgttc
     5281 caggtatgat tgctaaagtg actagtatac tttctgagac caatacgaat atcggcacta
     5341 tgacagtgac acgggaatca aaaggtgaaa atgccattat gataattgaa gtagatgatc
     5401 ctcaagtaga agaaacggta aaaagactca aacagctgcc aaatatcgac agcgtaaatt
     5461 atttcgatta aggagctaaa aaaatgtttt tatcgattga agaattagta gatcaagcaa
     5521 aagcttatcc aagtgtagca gaattgatga ttgcagttga aatggaaatg agtggccgtt
     5581 cccgtagtca catcatcgag atcatggaaa aaaacctctc agtaatggaa caatcaatca
     5641 acgagggaaa agaaggtgtt acctccgtca caggaattac cggtggtgat gcaaaaaaat
     5701 tagatgatta tttaaatgag gcagactttt taagtggaga aacaatctta aaagctgtac
     5761 gcaatgctgt agctgttaat gaagtcaatg caaaaatggg gttgatttgt gctacaccga
     5821 ctgccggaag cgcgggtgtt gttcctggtg tgctgatggc tgtccgagat cggcttgatt
     5881 tgacacatga acagcaattg gaattcttat ttaccgctgg cgctttcggg ttagtcatcg
     5941 caaacaacgc ttctatcagt ggtgcagaag ggggatgtca agcagaagtt ggttctgcaa
     6001 gtgcaatggc agcagcagct ttggtttgtg caaaaaatgg gactcctcat caagcagcac
     6061 aagcagtagc cattacattg aaaaatatga tgggactgat ttgcgatccc gttgcaggct
     6121 tagttgaagt cccgtgtgtc aaacgtaatg ctttaggctc ttctcaagct tttatctctg
     6181 ctgatatggc attagcgggt atcgaaagtg tcattccgcc ggatgaagtc gttgctgcaa
     6241 tgtaccaagt cggacgtcaa atgccgtcga ttttcaaaga aacagcagaa ggtggcttag
     6301 ctgttacgcc aactgctaaa cgacttgcgg aagagatttt aaataagagg gcgtgataaa
     6361 gctccttaat ttcgagtatt aagagattag ttatcttcat aacaaaaaac gaagagagga
     6421 ctaattgccc ttctcttcgt tttttattaa tccatcaatt tcgtattttt tacttcatcg
     6481 atcgtttttg ttcctgcaag ttgcatcgtg atcgacaatt ctttgttcag gtgttcaaaa
     6541 acagatgtta ctccttctgc cccacctaga ttcaacccat aaatcactgg tcggccaatc
     6601 gcaactaagt cagcaccact tgccagagct ttgaagacat gctctcctcg acggacacca
     6661 gagtcgaaaa tcactggtac acgtttgttg actacttcgg caatttttgg caatacttca
     6721 aaggacgcag gacctccatc taattgacgt ccaccatgat tggataccca gataccatct
     6781 gcccctgcag agatagcgat tactgcatct tctggcgatt gtattccttt tacgataact
     6841 ggaagatggg tgatttcttt gattgttttg atgtcatctg gtgtcaagcc ttgttttgca
     6901 gctgcataaa tttctgcgat tccttttcct tcgccattac cacttgcact ttgttcgcta
     6961 tacgcagcta agttaggcat tggtaatgga aattggaatt gattgatgac gtcttcttca
     7021 cggtaaccac cgagcgttga atctgccgtt aaaatgatag ctttagcccc tgcttctact
     7081 gctttgttca aaatgaactc attgaatctg tcgtctttac tcatatataa ttggaaaaac
     7141 tgcggtgcgc ctggcgctgc atcggctgcg tctttgattg tcgtatttgc ataagtgctg
     7201 atggaaaaga ttgatccagc agcagctact cctttagctg tatcagcttc ccctttttca
     7261 tgtgccaatc cttgggccgc tgatggcgct tggataatcg gtgtatctaa ttcaattccg
     7321 aaaacagatg tatgcaaatc ggctgagtcg attccgcgta atatccgtgg catgattttc
     7381 tttgtattaa aagaagtcgt attttctttc atcgtccact catcttcaga accaccgcga
     7441 atatagccaa aagctcctgc ttccatgcgc tcttttacac gaccttctag ttaggcaatg
     7501 tttacaatat cgattggatg ttcttcatta cttgtatgat aagtcatagt agatccttct
     7561 ttcatcttct tgattacata taaatcatac taaacttaca aaaagtaagc aaacatttac
     7621 tctcagatac ttttatcgct attctttcca aataaatcga cttacaagct gatctacttc
     7681 ttggttttca tgaagcatac tatgagaggc attttcacct gtcactactt tttcctctat
     7741 ttcatttccg cgttgcttca acaacgcata gaccgctaat gcactgttta acggtactgt
     7801 tccatcactc aagtctgttt catctagttg accagcaatc aagaaaaatc gagtggttat
     7861 aggaatatta ccgatcattt gctggtaatc ttgatagcgg ttagaaacga cagcaggccc
     7921 ctttgctaac tcatctgtca gagactgcgc actatcatcg acaaaatcat taaaaggcgc
     7981 gccaattgct gttaatgtct gaacttgtgg caatgacgta tcctgtccat acgttcctaa
     8041 atagcgtagt gcactaactc ctcccatgga atgccccact atgttgactt tatctatgcc
     8101 gtagttttct ttcagataca aaagacaagt tttgatccat tctgcttgat tccactcgtt
     8161 atttttatta tcttcaaaca atacttggac catcggattg tctgcttttc cagacaaatc
     8221 tcctgttgct tgtatttgtc catctgttcc tactgttaaa accaattctt ttactgcttg
     8281 ctgatttttt tccaaacgcc gcaacatccc tttaaaggaa ttttctgtcc cgctgtagcc
     8341 gtggaaaaaa agtgttggaa tagtggtttt ttctatcact ttactggatt ctgccgtttt
     8401 ttctttatca gaaacttcag tagtatttat cttcgtcgtc tggcaaccac ttaacgcaaa
     8461 caaactgata aggactaaag ctgctgcagt tgcacgtttg cataaatttt tgattggttc
     8521 tgtcatacac ctcttccttt ccatatagaa aaaagaaaga cttgaaaggc caatcatatg
     8581 atcgacattt caagtctctt tattaatcga ttttatctac ttgatcgaaa tcaagttctg
     8641 tactggtttc acgtccaaac atatcgatgt tgactttcaa tttttctttt tctccgtcaa
     8701 cttctgttac ttgtccttct aatccagcaa atgcgccttc gatgattttc acgacttcgc
     8761 caacttctac ttctagatca ttttgtcgca ggctcatacc gatagaacgc aagatatgat
     8821 tgacttcttc tggaagtaat ggtgctggtt tgcttccggc tccgtgtgaa ccaacgaatc
     8881 ctgtcacacc aggtgtgttg cggacaacat accatgaagc gtctgtcatg atcatttcaa
     8941 caagtacgta tcctgggaat gttttatgaa cgatttcttt ttctttccca tttttgactt
     9001 cagtttcttt ttcttcagga acaacgacac ggaaaataaa gtcggccatt ttcatacttt
     9061 gcgcacgaga ttcgatattt gcttttactt tgttttcata tcctgaatat gtatgcaaga
     9121 cataccagtt tttttcaaaa gattccattg aaggctcctc atttcattgt aaaataaaaa
     9181 aacctcagta cacccgaagt ttttctttcg cttcattata gcactaaaag tacgttgtgg
     9241 ctagcgagtt attttaaaat ccagccaaat aagctttgga tggctgtatc cattacgtaa
     9301 aacatcaatc cgaaaataat agaagtttcg atgactacta acgtatcttt acggagttgt
     9361 tttctgcttg gccaagttac ttgtttgatt tcatctttta cgctttttag aaatttcatg
     9421 agacaacctc ccttgctact gtttatttcg tttctttatg taatgtatat tgattacaat
     9481 atttacaaaa cttattgatt tccaaacgtt cgccgctttt tccttcactg actgattttg
     9541 tgtagttgcg agacccacaa acggaacaag cgagtgctgc ttttttcttt gccatttact
     9601 tctgccccct tgctgagaat cagacttata cttcataaca gtatcacgta actttttact
     9661 tgtcaatttg tgaaataaac tatctattgt tttggataaa attatgatat gattaatttg
     9721 aaattaagct aaaatgagac aattggaggt atagcgatgc tattaaaaac acttgtatat
     9781 aagaaaaaag atctgacaac cgttacagag actagtacat tagaagaagc attaaaaatt
     9841 ttagaaactt ccggataccg ttgtgtgccg atcttagatt cttcaggtaa tattttccgt
     9901 ggaaacattt acaaaatgca tatctatcgt cataaagcaa atggcggtga tatgacgctt
     9961 cctgtcactc atcttttgaa aaacgctaca aagttcattt acctggatac ttctttcttc
    10021 aaagtattct ttacgatcaa agagcttcct tatattgctg tccttgacag tgaaaatcat
    10081 ttttatggta ttttgacaca tagtacgttg cttaatatgc tctctcaatc ttggagcgta
    10141 gaacaaggaa gctatgtact gacaattgct tccacaggaa agcaaggaga tttggctgct
    10201 attgcaaaaa tcatttcaaa acacagcagc attgccagct gtattactct tgatatcgaa
    10261 aaagacgaat acattcgacg tacattgatc acgctaccgg ctaacactga agcgaaagta
    10321 tgtgaaacga tcgtcaacca cttagagaaa aagaattata aagtggtaga aattgaaaat
    10381 ttgcaaaacc aagaagcata atcaaaaagc gaaaaaagaa aacagctaat ctgctttctt
    10441 ttttcgcttt ttataattag acacgctttt caaaatcaag cgcgcgatct aaataataca
    10501 tcaatggtgc tgaaacagcc atgacgataa actcacttaa tgcaagcgtg ccataggttg
    10561 tccaaaatgc ttggccacct gttggaacta acatataagc aatcagtccc atgctgatgg
    10621 taaagaagat cgtgttcaat actaaacgca tctttacatt tttcactttg ttttgcaata
    10681 aagctgtcag gcctaaagaa agcaatgttt ggccgccacc aaaaaccaca tccatgatac
    10741 caaaaccaaa gaaggcatta tagacgatga ccccgcctaa aactccccaa agcaatttac
    10801 ggttaaagac aactaaatga ttcaaacttt ctgaaatacg aaactggatc ggccctgagg
    10861 atacaggcgc aactaaaata gttaacgcta gataaagagc catgatgatg ccatttacaa
    10921 cgattacgga taaccgttgt ctgcttttcg ttttttgcat tttctctcct cttttctcct
    10981 agttttttta acgcgggatg gttgatgaac cgcggttatg tgaaacaaac aataattata
    11041 acagactttt catttatttc aatccgtctt atctgctttt aaaaaagcaa aagcttaaac
    11101 aatgtaaacg tttgctaaaa atcgtgggat atggtataaa gtaagtgtaa ggggtcataa
    11161 gaattcccag taaagcagac gaagtaggat ctgcaagaac agaattgttg tgaaacttaa
    11221 ataaataaaa aaggatgtgg gaatttgagc aacctcttac tagagaacgg tctagcaagc
    11281 agctgaagat ctaagtggac atgccgaacc ttggaacttc tccttacgta agtaatggat
    11341 catgcgcttg ctaggctttt ttgtgttttt cggtcttcgg acctttttca ttttcctaga
    11401 cttctgctat aataaccaag aattaatttt gtaacagatt tttttgaaaa aacaagtttc
    11461 tttcattcaa aaaatagaaa aagcttgcta attattggtt aaccagcaaa caggagtatt
    11521 aaaatatctt gttttattga atagcaggag gaaatcatga aattccatta tcatatattg
    11581 atcaatccat cggcaggcag cggaaatggc tataaagtag cagaaagaat tttaccggta
    11641 ttaaaaaata agcatattga ctatacactt cattatagtg aatacaaagg acacgatgct
    11701 gagattgctg aaactttggc aaaagaaaca ttgattcctt ggatagaaga agaacagaaa
    11761 acaatcgata ctttccccct tttgatcatc gtaggaggtg acggcacctt acatcaggta
    11821 cttgatactt tctatcagat ggaagtagaa ttcccagttg cttatattcc agcaggttct
    11881 ggaaatgatt ttgctcgagg ggcaggcctt ccaaaaaatc ccaaaaaagg acttcagctg
    11941 attttagcag ctcaatcacc agaaaaagtc cacataatgg cttatgaaga aaaaataagt
    12001 gaaaaaaaag gaatagctgt taataatttc ggtataggat tagatgcagc tatcgtccat
    12061 gcgacaaatc attcttccac aaaaaagcga ttgaacaaat acaacttagg atcattttcg
    12121 tatttattct ctattctccg tgctttattc actcaaaaag gatttccaat tctggtagat
    12181 gcaggaggaa aacaattaag tttttcaaac gcatttttat gtacagcaac gaagcatcct
    12241 ttttttggcg gaggaatcgc gattgttcca actgctgatg cgtctaaacc agtcattgat
    12301 tttgtcgtgg tcgaacgaat caatttcttt aaaatcttgt ggctcattct tttacttata
    12361 cagaaaaaac aattgaaatc gaaatatttc catcactaca caacaaacag attacgtatc
    12421 gtttcaacaa caccgcaaca tggtcaagaa gatggagaag aaatggaaaa acgacctttt
    12481 gatatcacaa taaccacaac taaacagttg ttttggtgtt aataaaagag gttttgtcag
    12541 aagctacatc tggaacaccg tttattcgga aaaaaggact gtgacacgac ttttgtcaca
    12601 gtcctttttt tactatgcag ttaattcttt gcccaattgg gttgccatcg atagtttctt
    12661 tctttaccca aaataaattg gatcattgaa aacaccgcag caaaagcaac taagacacaa
    12721 ccattgaacc aaaaagattc tgcaaatgtt tgattgtaac tgagtgtttc actgatcaag
    12781 ttattatttt gaacaaacca atatgttggt gtgaatgccg ctattttgct aaccatttct
    12841 ggcaagtatt ctgctggtac aaacacccca ccgataaagc aactgcctgt gataaaaata
    12901 tctttgatcc caccgctggt ttctgagttt tttaccaatg aagtgataca agccgaaaag
    12961 ctaatgatcg gcaataaaaa caagaaagta tttaaaatat gataccatac cgttatatcc
    13021 cacgaggtgt ggatcactaa cagaaccatt agaagtaaga agccactgaa caacagcagc
    13081 gagtaactaa tcaaactaaa ggttactttg cgattcaagt ttcgtttggt aatgggcgca
    13141 ctctggtttc gtttactgat ctctttgcga ttaaacgcta gattgatgac gctgaaaccg
    13201 ctaaaaatgg atagaaacaa cccataagac aagaaattaa aggttgtgcc cgtcaacagc
    13261 tttttcatcc gctgactata tcccgcactt agctcaactt ctccagtaac cgagagtgcc
    13321 atttgtgtct gttccatagc tgcatcgatt gttttatctt cttcttgaaa cagaatcagt
    13381 gtttgcaaaa aggtgttcac ttgctgtgta acaactgttt tagaaaaggc atctgggcga
    13441 ctctgtatct tgatcgtcgc catctttcct acttcgactt gttcggtaaa gtctgcaggc
    13501 agatgtaaga taatgtttac acgattaaaa taaagagcat catcgatttc tttttgtgag
    13561 gttccatcca atgtaacgac tgtttccttg tcattcaaat atttacgaag gccatcgact
    13621 acaggcgact cttcttcact aatcaaggcg attttcgcat cttgcagaga ggtattttca
    13681 acatcaatat tttgcgcaaa aaagaatgtg gcaacaacag tgataattac acctatcagt
    13741 aaattcgatt tatttgcttt aagcaatgtc caaatcgttt tataaagcgt cataacgcac
    13801 ccctctttcc atccaagcat ttcctaacgc aaaaaggaaa ataaatccga tcaaccatag
    13861 aagattggta taaaaactag ccaaatcagt gtagaaataa agtttataca aactttcgct
    13921 gatcaagttg acgagattta attgaccaac aatcggaata tgaagatcaa tccaatactt
    13981 cacttgttcc gaccccatca taccagcaaa aaagctcatc gcaatcgtca ctgaaatcaa
    14041 gatgctatcc ttttgcacca gattcatttt tggtatcaaa ttaccaacca atgtacctaa
    14101 caaaatcgca cacaacgacc ccagagcggt aacaatcatc aaccattgcc aacgttcacc
    14161 aaagtctact tgatagataa aacgaaagac agctaacata atataaatct ctgtcaaaac
    14221 aatcgtaaag cttgctgcca aattagcgat tgaaactaac gattgctttc taggaatcaa
    14281 ggacaagcga ataccatttg acgattgatt tgcttgctga tcctgtacat tgcggacccc
    14341 ccacatcgtc ccgtagagaa tcgccattcc aatcagagtg aagaaataaa aacttttgaa
    14401 actcatatta cgacctgcat gaacttcttg aaaagatact tcttgttcaa aaacagtcat
    14461 gatgggcgcg agatcaacgt tctccacttc tgccacttta ctcgaaatta taccaatacg
    14521 ctgtaaaaat tgatccatga cgtttttcat caacgtctga ctcattcctg cttgacccac
    14581 atggagcgaa acagatgaac catcaatgag ataaaaacca gccaaatcat ctgcatttag
    14641 cttttcattc gcttctactt ccgatagttc ctgtacttca aataatggtt tatcattgac
    14701 ttcgacttgt tctaagactg tggtaaaatt ttccgaaagc gctgtttgat ccgctttgat
    14761 tacaattcca gtattaatcg tttcaagatt gccaccttga tcaatctcgc caaaggccgc
    14821 cataaacatc aatcctaaaa gaattggaaa ggccaatgtc caaaacaaca tggatttatt
    14881 tcgcagcagt atttttgtgc gatatttata aaaatgccaa aacattattt cccctcctct
    14941 agtctctcaa atccttgcca gtaatctcta aaaagacatc attcaaggac gcttctttgg
    15001 ttgctatttt tccgtaactg atcttttctt cttgtaagat tgtaagaatc ggcaacaata
    15061 ctgtatttag ttcagtagtt gttacttgga gctgaccttt tttataagcg attccatcag
    15121 aagctattgt tcgctgaatc gctgacaaca cattttctgg gagtaatggg atgtcgatcg
    15181 tcaaacgatc cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca
    15241 cctgtccttg atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat
    15301 gtgttgtata gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt
    15361 tattccgact ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct
    15421 tgtgggcaat cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg
    15481 gacgaaacga aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt
    15541 tccttttctc tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac
    15601 ttagttcgtc aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat
    15661 aattttcagg agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca
    15721 aagaaagcat acagttgatg gcagtcgatt tcccactgcc gtttggacct aacagaccta
    15781 agacttctcc ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc
    15841 gtttgactaa tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt
    15901 gatctgacta taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg
    15961 acatttgtca caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc
    16021 tttctactgg ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt
    16081 atctatttat tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca
    16141 aattggattt tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg
    16201 tttctgccag cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt
    16261 ttgttaggtt ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt
    16321 gtcgtaattt tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat
    16381 cttgagaaaa agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg
    16441 gaacaaaata aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa
    16501 gaacggaacc gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg
    16561 attttacaaa ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta
    16621 gaaaatttaa gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac
    16681 ctccatgaga catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct
    16741 tcttctgtta cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc
    16801 ttcttaatgg tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc
    16861 gttgttttac attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc
    16921 accagcccac aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt
    16981 caagaacaaa agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc
    17041 attttaccaa gggagggaca ccaatgataa cagtagtcat tattgatgat gatccatttg
    17101 tgacgaaatc gttacaaacc attttagaaa gcacgaaaga gatccgtgtg ttaggcattg
    17161 gtcattgtgc caaggatgcg ctagtcttat acgaaaccca tcgtcccgat gttttgctga
    17221 cggacatccg catgcctaaa caaacgggca tcgatgctgc caaagaaatt ttggctaaat
    17281 ttcccaaagc catccttttg cttttgacga cattcaaaga tgaagaatac atccgcgaag
    17341 cattttccat cggtgtcaaa gggtatttaa tcaaacagaa cttgcaagcg attattcctt
    17401 ctgtcaaatc cgcttataat ggacaggccg tatttggtaa tgaaatcatc gagactttca
    17461 ctcaaatgat gaagaataaa ccgacgattg atagtcatgc ttatgcttct tttagtgaac
    17521 gtgagcttgc aatcatcaaa gaagtagctg ccgggaaaaa caataaagaa attgcagatg
    17581 ccttgtactt gagtgacggc actgttcgca attacatcag ccaattacta gaaaagctag
    17641 atttacggga tcgcacgcaa ttggctatct atttttatca gcatttgcaa taagtcttat
    17701 ctaacacaaa aaaacctgaa tgatgctcct tcgcatcatt caggtttttt tcacatcttc
    17761 tattcaattg tcccgcaaac catttcctca cgattgaaag aatcaaggat tcgcagggct
    17821 gccaggattc gttggattgt tcggcgtgtt aggcggaaaa ataggcgcct tgtctaacgt
    17881 tttttctagt tttgattctt cacgatcgtc tcgttctaca tcaggacttt cattcaattt
    17941 gaattctttt tccatctcat ctaacttttt aaagtcattg ccagattctt ttgctaattt
    18001 cgctaattta tcttcggttt ctttagccat aaagtaacgc cccccttttg atttcagctt
    18061 agcatatcac actgaaaaaa caaaagaaaa cgctaaccaa cgatcatttt tagctatttc
    18121 tcatctttaa aatcaagcat tttttgccgg tataaaatcg tagggagaac tgttttccat
    18181 accaatcatc ggatctacta actggttatc gatcacttct gctgtaagaa cgaaatcttt
    18241 agaaacagca ggttctacct cttctttctt ttcttctcca acaaatgaag aaaacagatc
    18301 tggttccact gtttccatta cttggttttt cgtttcattc gtcccactgt catcttctac
    18361 aggtagcttt tcttttacaa cccgtggctc ttttgtatag ttcccatctt caaatggatc
    18421 atttccgacc aaagaatctt cataagctgc tagtttggtc cggagagtga tattcatttt
    18481 atcattatcc atgatccttg atttcaacgt agtcatccgg cttgctgatt cattcgctac
    18541 acaagtgaca taggcttgct cttcccctaa caaaatcaac agttccttac tgcgagtcac
    18601 tgccgtgtag agcaaattac gctgaagcat ccgctgatat tgatggacca tgggcaaaat
    18661 aaccatcttg aattcactgc cttgtgattt atggattgag caacaataag aaagagtgat
    18721 tttattccat tcgtttcttt tataagtgac ctcatttgca tcaaactgga taactaattc
    18781 atccacttta tcttcagaat cttttgccaa gatgataccc gtgatttctc ccatatctcc
    18841 attgaataca ttcaattcgg gagtattgac taattggagg actttgtctc ctatccgata
    18901 aacggtgtcg ttccaatgta cttccttttt tccaggtttc cctggattga atatctcctg
    18961 catcatttta tttaacgcat cgatccctgc aggtccccga tacattggtg ctaatacttg
    19021 gatatcttgt gcacggaatc ccttattttt cgctttttcc acaattttag agatcaaggg
    19081 ttcaatttga taagcatcac ttcggaaata ggaacgatct ttttgattct ttgtgaaatc
    19141 ttttggcaga cgtccttctt tgatctcatg ggcaagagga ataatactag aaccatcacc
    19201 ttgccgataa atctgattga gttccatttt gggaatttgt tcgatttcca aaagatcatg
    19261 gagtacttga ccaggaccga cagagggaag ctgatctttg tctcctacta agataacttg
    19321 catattcgtc ggaatcgctt tgaataatgt gttagccagc catgtatcga ccatagacat
    19381 ctcatcaaca atcaaaagtc cgccttctaa ttctttcgta ctgactgaag gtgttttttc
    19441 ccgtccattc agccccagaa gacgatggat cgtactggca ggcagacctg tcgtttcgtt
    19501 cattcgttta gctgctcggc cagttggtgc tgcaagtaga ataggaaaaa tttcttgggt
    19561 atattttgaa ggatccaatt ccagtccgtt caactctgca aaaagattga cgattccgtt
    19621 gatcacagta gttttccccg tacctggtcc tcctgttaaa ataaatagag gggaacggat
    19681 tgcttcagcg atagcttgtt cttgcgaagg tccatactgt atgccgaata ttttttcgat
    19741 acgccgaata ttcttctgca cttctttttc aggataatgg atttcttttt tacgactgag
    19801 aaggcgctgg atcgaattac cgatccccca ttcagaaaag tacaaactgt tttcgtaaag
    19861 attagtctct tcttgttgta tctttccttc ttcaacaagt tggataatta cagtagcaac
    19921 ctgatcaggc tggatttcaa ttgggcgact tgtttctaaa atacggatcg tttgctgaag
    19981 caatgtctcc gccggtacgt atgtatctcc tgtttgaact gcatgctgaa aaatctgatg
    20041 caagatagct gctcgaattc ttctcggtga atctgctgca ataccgattt gttcggctag
    20101 attatctgca cgtttgaacc cgattccttc tatgtcttcg actagctggt acggattttc
    20161 ttcgatgata tccaaagact cgttcttata ggcctggtag atactgaaag ctaactgact
    20221 accgaacccg taacgattca gtcccatgat cacttgatcc atcccgtggt ttagccgaat
    20281 cgtctcagca agcatttctc tttttggctt cgtcaaaccg gagatttgtt ttaataccga
    20341 aggatcagca atgatttttt cgatagcatc ttctcccagc aattctacaa ttttttctgc
    20401 tgttttcttc ccgattcctg ggaatttttc gctggaaaga tacgctacga tcccatttgc
    20461 agttgttggc ttttcttgct catatctttc gagtttaagc tgccttccgt atttaggatg
    20521 atccactaat tctccataaa aacgataagt ctcattttct tgaatatcgc cgaaacttcc
    20581 ggtgactaca atctcttttt cacggtaatc agctgtggtt tcactgatgt gtaccaaaag
    20641 cactttgtaa aaattactag gattttgaaa aaaaatcgcg gcaactgtac caacgaaata
    20701 aggcatttct tctggctgca tcggaagccg cctccttctt ctatagtaaa ggttctggtg
    20761 ctgtttcagt tcctaaaaaa tctgtttggt tccatacttt cagttcatat ggcaataatc
    20821 tgcaaggctc ttcagtctct aaaatcgtca aactactatt gaacaatcca cccatttcac
    20881 gcaactcact tagctctttt cctgtcatcc attgaatggc tgcagttaaa gaagctccat
    20941 gtcctacgaa taaaagtggg ccatctttat gtcgggcaac cgcatctgct acaacagttt
    21001 cgatacgtgt gattgccgat tcaatcgttt ctccgtgaaa aggagaagga tcatacagat
    21061 ctaaatgatt acgcaaattc ggcagattat ccggatattt ctggtacatt tcatcgatcg
    21121 actgaccttc taaagtaccc agccctagtt ctcttagctt atctgtataa aaaatctcta
    21181 ccggacgagc aagctctttg ttgatacctt ctgctgtatc acgtgctcgc tttgctgtac
    21241 tggaataaat tgctttaaaa ggaacatatt ttaccttttt cccaaatgct ttgatttctt
    21301 cgtaactttg tggcaaaagc ggagaatcgc cattgctccc ttgaaaacgt ctttcctgat
    21361 tccattgtgt cttcccatgt cgggtaaaat atagttccat acgcagcaaa ctcctgtctt
    21421 tcttttattt tactctataa taaactgtct aatccagctt gtcattttca tagtgatgcg
    21481 tcaattctaa attagggaat ccttgctggc gaagtgcttc atacactatc attgcagctg
    21541 tattcgataa attcaatgag cggacatgct catcattcat agggatgcgt aaacactttt
    21601 cttcgttttc acgcataaat gtttctggca aaccggttgt ttcttttcca aataaaaaga
    21661 agtgctcttc tccatcagca aaattttcgt cactatatac tttatgggca aattttgaga
    21721 tcaaatgtag tttacgatta cctaaaaatg ttaaaaaagc ttctaaatta ctgtggtaag
    21781 taatatttac atcattccaa taatccaacc ctgcccgttt tagatgtttg tcatcagttg
    21841 aaaaacctag tggctcgatt aaatgaagcg gagtattcgt ggctgcacac gtacgggcaa
    21901 tatttccagt gtttgcaggg attaatggtt caaataaaac gatatgattc atcaggatca
    21961 ttcctctcat atttatgttc gtttctattt tctgacaatt tgatttttgc ataaaaaaaa
    22021 acgtatcaac tcggtgtcag tcactgcgag tcgttacgtt agtgaagctc ttctcactaa
    22081 cttttatcag aacaggaacc tgataaaaac caatgaaaaa acaacttgtt gctaatataa
    22141 caccctcaac ttttttttgc aacctctact cattaaaaac tgctagaaaa acgttttctc
    22201 ttagttcctt ttaataattc tatgtcaatt gtaatcttct tcaaaggatt cttatataag
    22261 tctgctttga ttcgctcttc tacctgccat tctaaaggtg acatggccaa aagatctttt
    22321 tgattttcct ctggtatctc aaattcatag gttacacgct gacgttcagt taatttcatt
    22381 gcttcagcaa acttggctac tacacgttca ttggaataac tctgtttttc aggttgattc
    22441 ggataaaaag cctgacgtag ttcctttaaa taattttctt gtggcaccac cttcaacact
    22501 tctccatctt ctgagagtac acgttggaat tcttggtaat gagaaggcga aaagatattt
    22561 aaaaccgcgt tgaatgtttg atcagcaaac ggcaaattgg tcaaatcagc tacacaccaa
    22621 aatgcttcgg cagattgttc tgtagctgcg tagactcctt cttttgaaat atcaaatcca
    22681 acagcatttt gaatcttttt caattttgaa agttcattca agaagctacc ttctccgcag
    22741 ccaacatcta atagcgagac ttcttcaggt aaatgctgcg caatagtctc aataacgggc
    22801 tgatacatgc cagattggat cattctacgc cttggaacga acattccttg atcgtattct
    22861 gttttaaccg catgggataa aaaataaagt gttccttttt tggaaaggtc aaaacggtga
    22921 ttatttttgc atatcaagcc attatcagac ttttttactt cttcttggca aaccggacag
    22981 cggaacagct gatgattttc tgccaaaaac tggcgtcctc ggtcaatttt ttttaacata
    23041 gttccccctc atttctgttt tatcttaccg ttagatgctc actttggcaa ctgctaaaaa
    23101 tatgatacaa tgcctagaag aaagtgaaag ataaaggaga agattagatg atcaaagaat
    23161 tttgcgctga aaactacaca tctattcctc tagcaattgc tagaggagcc gatcggatcg
    23221 aattgtgtga caacttagcg gtcggtggca caaccccgag tactggtgtg atcgaagaaa
    23281 cgttaagtta tgctggggaa aaagatatcc ccgtaatgac gattattcgt cctagagggg
    23341 gcgacttcgt ctataacgat atcgaattga aaatcatgca tactgatttg attgaagcaa
    23401 agaaattagg gacggatggc gtagtatttg gttgtcttac tccttcaggc tggctagacg
    23461 aagaggcctt agaactattg atcgaaacag cagaagggtt gcagatcacc ttccatatgg
    23521 cttttgacag tattcctgaa gatcggcaat tcgaagcaat cgattggctg gtagaacatg
    23581 gtgttgaccg tatcttgact catggcggtc ctgctgacaa aactattgta gaaaatttcc
    23641 cacggcttaa agaattgatt gcctatacag atggccgctt aatcattttg cctggtggtg
    23701 gtgtcactgc agagaatgca gcacatgtag ctaaagaatt gaacgtaagc gaagtacacg
    23761 gaaccaaaat cgtggatctt cagttataat cttataaaca aaaaaacatt tgcaacaggc
    23821 ataatgctta ttgcaaatgt ttttgttttt ttacataaaa tagcccataa ataaagaagc
    23881 aatattgaaa taaatcagta cactcgtcaa atcgctcaaa gtcgtgataa agggaccgct
    23941 tgctactgct ggatcaaatc ctagacgatc cattagcatc gggattaaac tacctgcaag
    24001 attcgccact gtgattgcaa atagcatagc catccctatg acaaacccca agataaaatt
    24061 atgtttccaa aaacctacga ttagaaaaat cgtgatcccc gtaacagctc ctgtgacaac
    24121 gccggtcaag acttcagcca aaacaagtcg aacaaatcca tgatctttct catcagaaat
    24181 cgcaagtcga cgaacagcca cagctagcga ctgggttcca gcattaccgg cagttcctgt
    24241 aatcagcgaa atgaagactg ctaaaatact cgcttcactt accaagtctt catagtgact
    24301 gattaaagaa gcggtcgaca tacctaaaaa caacagagtg atcagccaag gcaaacgtcg
    24361 agacgctgcc tttagtggat tttcattaat ttcttccaca ttgaccccgg caagtcctga
    24421 ataatcgcta gcagcttcat catcaataac gtcgatgata tcatcgactg tgacgattcc
    24481 taacaaatga tcatcataat cggtaacagg aagagctaag aagtcataat ctctaaatgt
    24541 ttgtgccaca tcttcttggt cgtcgcctac atgaactgaa agcactcgtt cgctcatcag
    24601 atcagaaatc attgcgtcgt catcattcac gattaggtcg cgcagagaaa taacccctac
    24661 tagctgacct tcttgattaa cgacatagat gtaatagatt gtttcagcta catctgcttc
    24721 gttcttcagc acgtacatcg cagagcgaac agtttgattg gctacgatcg aaacaaattc
    24781 agttgtcatg atcgcgccag ccgtttcatc ctcgtaatgg agaagttcct tgatttcact
    24841 ggcatcgtct gccgacatca aacttaaata tttagcaatt tgctttttat ccagcgtatt
    24901 aagcaagtca acagcattat ctgtatacat ctcagaaagc atatcagctg catagctagg
    24961 acgcatttca tctaggtatt ctttcatatg ttcgttgtct tcttcaatca catcgaacat
    25021 atccgctaat tctttaggcg acagatagga atagatcagc tgcctgttct cttctgtaag
    25081 agattggtag aattgcccct gctcatagat atgcatctct aaaaaactgt ttctgaatgc
    25141 ctgaatatta ttttccctta aatcttgagt caactgggca aatctttcct ctaattcttg
    25201 attttcatcc aaaccaatct cacctcgcct tattcttcaa accgtctcac aactgcctcc
    25261 atgtctgcgg ctaaaggctg cttgaactcc agcaactctt ctgtaaatgg atggtaaaaa
    25321 cgcagatcat aacaatgaag ggcttgtctt tttattccca gatccattcg cccgccatac
    25381 atatcatcac cgagcagggc acagccaatt gcggaaaaat gaactcttat ttgatgtgtc
    25441 cttcctgtat gcagctggat atcaactaac gcgatttctg ttagtcgctt ctctatccag
    25501 tactcagtct gtgccgaacg tccttcatca gagaccattc gcttcaatag cgaagaataa
    25561 tctcgggcga tcggcaaatc gatatgtcca tgtgccgata aatctgaaag ccttccactg
    25621 actaaggcct gatatttttt cacaaacttc ttttcacgaa gttgcatatc taattttgcg
    25681 tgcgcaaatc catgctttgc aaaaagcatc aagccagatg tatcccgatc tagtcgcgtc
    25741 accacatgga ttacttggtt ttcgtaacct tgttttttat aataagcctt tacacgatta
    25801 gccattgtcc catttggatg gtattgggca ggaatagacg aaacgcctgc tggtttattt
    25861 actaccagaa cgtgttcatc ttcatacacg atatcgatcg gtgtttcatc caaaagtacg
    25921 gtttcatgtt ctccttcagc aggaatcaca atggttacct tatcatcctt agctagagaa
    25981 aacaagacat tttctacttg atcattaact ttgatttgac cgccttgaaa ttttattttc
    26041 gctaacagac cctttgagat ccctttttct tttaaaaaat attttacttg ctgagaatga
    26101 tctttctcat aatcccatgt aaactgcata tcaatcctca ctaataaagg catctttcac
    26161 acgatgccaa aaatgcatat gtcgataaga agcaaaatgg atccgttctt ctgctattcg
    26221 ataataaatt gagcggatat ttgcttttgc gacattcaat tgatcaaccg ttacaagata
    26281 atcttctgta ttttccaatt ttatttctag ccattcatca tgggcaatca cgatcggaga
    26341 acccaaagta cgaaacactc gattattcag tgaagcaatc tctgccaact gaaaggcatt
    26401 gatactagga tggatcacag caccgccgac acttttatta tatgctgtcg aaccagtagg
    26461 cgtagaaatc gtcaatccat ctcctctaaa actttcaaac agctcgtttt tgatgtatac
    26521 atcggctacc atcgtacgat tggcccgttt aatcgttgat tcattcagtg ctaaaaaatg
    26581 ctggtccggt gtttcatcca aataactgat ccgaacatcc agcaagggat aactaacact
    26641 ttgttcccgg ttcgttcgta agctgtctac tagttcttcc agctcgtaat cccgccaatc
    26701 cgtgtaaaac cctaaatgac ctgtatgcac gcccaagaaa cgcacatcat tcagacaatg
    26761 actgtagtga tggaacgccg acaataatgt gccgtctccg ccgactgaga tgaccaattc
    26821 tggctcatga tcatctcgtt gaatccctgc ttgttctaaa agtgcgttca aacgttctgt
    26881 cacttctata gtctttgctt ctttattatg aacgatcgct actttcatcc cctgcctcct
    26941 ctctttcgct ttctttcttc aaaagcgttc gatagtagac atcatctgat tttcctcttc
    27001 catgggagaa taaccgctgt gcttcttgga tttcttcacg gatactggac atctcttcat
    27061 ccaactgata ggcagcttct gccgcacgct gaagtcgttc actcatttct tctggaaaaa
    27121 cgccttgata tttataattc agcgaatgct caatcgttgc ccagaaattc atcgcaagag
    27181 tccgaatctg gatttccgcc aaaattttct tctcaccgct gatcagctgt acaggatact
    27241 cgatcaccag atgataagaa cggtagccac ttgctttttt atttgtgatg taatctcgtt
    27301 cttggagaac tttcatgtct tttcttttac ggatgatctc aacaacttga tggatatctt
    27361 ctacaaactg acacatgatc cttaacccgg caatatcaga catttcttct tctaaacgtc
    27421 ccattggaat atgccgtaat tgtgattttg ttacaatact atcgactggc ttgactctcc
    27481 cagtgacaaa ttcaatcggg acatgtcgat tttgttcacg gaactgtttg cggatccccc
    27541 gcagtttcac tttcaattct gatacagttt gttcatatgg tgctaaaaat gcttcccaat
    27601 ctcgttccat agctgatacc tctacttcat taacattcat tgtttattct accatatatg
    27661 caaagttttt tcgtactctt gatttatgca ttgaccaaaa aaagaaaatt aggcaaaata
    27721 gacaaaaagg agtcgatcaa atgacacaaa tgcttgaaat cgaatataaa tctatgctga
    27781 caaagcaaga gtacgagata ttcatcgctc actatcaatt aacagataaa gatttccgtg
    27841 tacagactaa tatttatttc gataccgttg atgaacagct gaagaaacaa aattgtggcc
    27901 tacggatccg tttcgttggt catcaagctg aatatacatt aaaaacacct gctgaaaaag
    27961 ggcgtctcga aacaacggat acctttataa cagaagaagc agaagcattt atcttcgaaa
    28021 aacgtctgcc tcgttcaggc gcagtttttc aaaaactttc tgatttgaat atcgatcctg
    28081 cttcattaat ccaaaccgga aagcttacaa caaaacgagc tgaattccct attgaagaag
    28141 gacttttagc tattgatgaa agctggggag acaatctaca cgattttgaa ctggaactag
    28201 aggtagggga tgcatctgtg ggaaaaattg cttttataaa ttttttaaaa cgtttttctt
    28261 taccttatcg tcctgctaag aacaagatcc aacgtatgct tgaagctaaa aattagaaac
    28321 ctttcatttt cacaaggcac ttgagttcat ttttttactt ttttctgtta agttatagga
    28381 tgtgaaagaa tctagtcact agttgtatag aatctatttt gggggagatt catatgatcg
    28441 aaatctattt atttgttaat cctttagggg aatactgttt cgaaatggaa caacagttat
    28501 tacaatttat cgaagaagag tacgggacac catcaaaaga aaagatgcaa tttcgctttc
    28561 ttcctttggt caatttgcag acaattgggg atgtcatgca acaaaaagga atttctcaga
    28621 acaatcttga ggaacgtaat cacttattct caatgactta ttctgcagca ttggactgca
    28681 aagcggcgca gtttcaagga aagaaaaaag gccgccgttt tttgatcaaa ttgcaagaag
    28741 cagtcggctg taatgacata ccatattctc aagaacttgc tgaatccata tttgaagaaa
    28801 tcggcggcga tttgaatatg tttaaagaag atcgtcagtc tgacttcgtt aaagaagtat
    28861 tcttttctga tcaaaaaatt gctcgggaaa tgggcatcac aaaacaccct tctgcagtcg
    28921 tttacaatta tgcctgcgac cgtgattttg gtgttttagt agaagtaaaa ggtacaagta
    28981 gcctttggag aatcacagag ctgtgtaaaa caacagataa ctcatatcaa gtttttaatg
    29041 gtgaacattt acatacaaat gattctcagt tccattcaag aactagccat ttacacttgc
    29101 tttctaatta aaaa
//
LOCUS       DACESX010000023        28295 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.023, whole genome shotgun sequence.
ACCESSION   DACESX010000023 DACESX010000000
VERSION     DACESX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.023"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            34..1374
                     /locus_tag="IXF88_000876"
     CDS             34..1374
                     /locus_tag="IXF88_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1507621.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1569..2012
                     /locus_tag="IXF88_000877"
     CDS             1569..2012
                     /locus_tag="IXF88_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1507622.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2274..3692)
                     /gene="nhaC"
                     /locus_tag="IXF88_000878"
     CDS             complement(2274..3692)
                     /gene="nhaC"
                     /locus_tag="IXF88_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1507623.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4096..5319)
                     /locus_tag="IXF88_000879"
     CDS             complement(4096..5319)
                     /locus_tag="IXF88_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1507624.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5351..6190)
                     /locus_tag="IXF88_000880"
     CDS             complement(5351..6190)
                     /locus_tag="IXF88_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1507625.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6207..6887)
                     /locus_tag="IXF88_000881"
     CDS             complement(6207..6887)
                     /locus_tag="IXF88_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1507626.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(6889..7962)
                     /locus_tag="IXF88_000882"
     CDS             complement(6889..7962)
                     /locus_tag="IXF88_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1507627.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(8612..9283)
                     /locus_tag="IXF88_000883"
     CDS             complement(8612..9283)
                     /locus_tag="IXF88_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1507628.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(9371..12049)
                     /gene="mgtA"
                     /locus_tag="IXF88_000884"
     CDS             complement(9371..12049)
                     /gene="mgtA"
                     /locus_tag="IXF88_000884"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1507629.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWFFITVVAYLALVTAVKKIYIRRFQELL"
     gene            12709..13146
                     /gene="ndk"
                     /locus_tag="IXF88_000885"
     CDS             12709..13146
                     /gene="ndk"
                     /locus_tag="IXF88_000885"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1507630.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(13168..14034)
                     /locus_tag="IXF88_000886"
     CDS             complement(13168..14034)
                     /locus_tag="IXF88_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1507631.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(14268..16346)
                     /locus_tag="IXF88_000887"
     CDS             complement(14268..16346)
                     /locus_tag="IXF88_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1507632.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            16839..18056
                     /locus_tag="IXF88_000888"
     CDS             16839..18056
                     /locus_tag="IXF88_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1507633.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(18515..19138)
                     /locus_tag="IXF88_000889"
     CDS             complement(18515..19138)
                     /locus_tag="IXF88_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507634.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(19294..20691)
                     /locus_tag="IXF88_000890"
     CDS             complement(19294..20691)
                     /locus_tag="IXF88_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1507635.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(20713..21684)
                     /locus_tag="IXF88_000891"
     CDS             complement(20713..21684)
                     /locus_tag="IXF88_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1507636.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(21691..23991)
                     /locus_tag="IXF88_000892"
     CDS             complement(21691..23991)
                     /locus_tag="IXF88_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAQ1507637.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(23993..25318)
                     /locus_tag="IXF88_000893"
     CDS             complement(23993..25318)
                     /locus_tag="IXF88_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1507638.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            25549..25974
                     /locus_tag="IXF88_000894"
     CDS             25549..25974
                     /locus_tag="IXF88_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507639.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(26472..27173)
                     /locus_tag="IXF88_000895"
     CDS             complement(26472..27173)
                     /locus_tag="IXF88_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1507640.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(27189..27629)
                     /locus_tag="IXF88_000896"
     CDS             complement(27189..27629)
                     /locus_tag="IXF88_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAQ1507641.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(27629..28204)
                     /locus_tag="IXF88_000897"
     CDS             complement(27629..28204)
                     /locus_tag="IXF88_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1507642.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         8206 a         5972 c         4881 g         9236 t
ORIGIN      
        1 tttgattgtg aatttaaaag aaagaggtta tttatggaag ataaaaaatt gaaaaaacaa
       61 cttagctccc gacatatcac catgctagct ttaggtggag cgattggtgc aggtctattt
      121 aaaggaagtg gcgaagcgat tggaatcgcc ggcccttctg tattgatcgc ttttttgatt
      181 ggcggcataa ttttatatat cgtgatgaaa ggtctgggaa aaattgtttt aagtggtggt
      241 gatacgcacc acggattatc tggattgatt cgtccatatc taggtgctca tgctgccgat
      301 tttaccgatt gggtctactg gtccatgtgg attatcaata tcattgctga agctgttgca
      361 gctgcttcct ttttacagtt gtggttcccc cacatcccag cttgggtatt tgtttttatg
      421 ttggctgttt taactactat catcaatctt tattcggtac gattattcgc agagacagaa
      481 tattggctgg cctttgctaa aatcagtgtc attatcctat tgatcatttt tgctgcttat
      541 cttgtcggtc agcaaatgtt gggtactggt gttttcccta cccttcaaca attaactgat
      601 catggtggtt ttactcccca cggtatgaag ggaatcatca gttctttgct tgtagttatc
      661 tattcttacg gcggttctga gttgattgct atcacagtaa gcgaagcaga tgatcctaaa
      721 cgtgcgattc ctaaagcaat caaaggcgtc atgggaagaa tcatttcctt ttacattatt
      781 cctttatttt tgcttttgat tatttttcct tggaatactt tagcaggcac gaacgttagt
      841 ccttttgtga tggtatttga aaaaatgaat attccatttg cggcagatat cgtcaatttc
      901 gtgattgttc ttgctctgtt ctcgtctatc aattcaggtg tatatgcttc ttcgagaatt
      961 ttgttcttcc gcttgaaaga tcggaaaggt tcctctagaa aactagctgt tttaaacaag
     1021 catcaagtac cgcaacgtgc tgtgctgttt tgtaccagtg ttctttatct aggtgtggct
     1081 ctttcttatt ttgtcggtga caaattattt ggctatctgg caggttctct ttcctataca
     1141 gtattattga tctggatcat tatcagtgct gcgtcctttg tactgacact aaggaaagga
     1201 acgttatggg acaaaggaat cagctttttt gcattagcag tcttatggtt gattttcctt
     1261 ggtatcctat tcacgaactc cattggggta actgtattga ccggtttgct ttacctgttc
     1321 atttatttca gttatcaaaa aaagaatgat gctttcgtct tgacgaatga atgaaaccaa
     1381 tagagaattt tattctattt atattgaata tctggagcta ttgaaaaaaa ggagctgcga
     1441 caaaaattgt tgcagttctt ttttccgaat aaacggtgtt caaaaaccat ctgtgcagat
     1501 taattgattg ttctccacgc tcatgacatg atagaagaaa ggaatgaaga aaaaggagtt
     1561 tgttttaaat gaaaaagtat tggaagtttt tgttgatgat tggtgtttcg acggtagtca
     1621 tgtttggtat gatgtatttg aatgtttatg cgcttgatca tatcttcttc agtcgcacac
     1681 gtgtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgttg ttgttcatgt
     1741 ggaaaatgta tgaaaacaaa aagataaatg ctatgatttt agccgctagt cttcttgttt
     1801 ttgccggttc cttatttatg gtacgaagcc aaacaactgt caacgatacg gcgtggatga
     1861 aggcgatgat ccctcatcac tctattgcta tcttgacaag taaacgagct gatttaaaag
     1921 atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa gaaatcgaca
     1981 aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc catccaaata aaaaaagagg
     2041 atgttccaga agtgatcaac ttcaagaaat aagaagaagc tacttctgga acaccgtgaa
     2101 tcagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc
     2161 ttctcatatt tttcgtttgg ttaggcttat taccgcaaat gcaagctttt gaacaccgtt
     2221 tattcgtaaa agggagtgtg acaaaagtct tgtcacactc cctttttacg tttttattgt
     2281 tctaattgcg ctgtcttgct tactgcttct tgcggtttca caaatttctt tgagatcccc
     2341 caacctgtaa aggcactgac catcgaaaac acaggtgaaa gcaaactgaa aaacacgaat
     2401 ggcaagtaag tgagtgtagg aatgccaaat gtacttgcta cgaaacttcc ggcgattccc
     2461 caaggcacta agtagttgat tactgttcca ccatcttcta gtgttctccc taatacagtt
     2521 ggatcaaatc ctgctttttc gtaagtttct ttaaatgcat taccaggcag gataacagat
     2581 agaaattgtt ctccaataaa gatatttact ccgatgcttg tcatcaatgt tgtaaaaatc
     2641 aattttgggg tgttcgtcat ctttcttgat actcgttcca taacggttga gatcaaccca
     2701 aattcgatca acaaaccacc taatgacaat gttagaataa tcaatgacac agttggcatc
     2761 atgctttcga ttcctccacg gctaagtagt gaatcgattt gttggtttcc agtatttgaa
     2821 acaaatcccg attcgatgac ccctgccaac ttcgttaagg aagtttgtgg gttttgaata
     2881 aagatcattc ctactgctac agcaatattc aacaaaatcg tcaaaattgc cggtaccttt
     2941 ttccatgcac acaagagcat taaaaataga ggaatcagtg accagatcga aatagtaaag
     3001 tttgcttcta aaatatcagt cacttcacga atagatgaaa gacttgcggc tttacttgtg
     3061 tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg ccggaacagt tgaccacatc
     3121 atatgcttga tgtgtttgaa caaatcggca tcaacaatag ctgctgctaa atttgttgat
     3181 tcggataatg gagacatctt gtctccaaaa acggcacctg aaacaatggc tccaacaaca
     3241 agggctggat ttagtcctag tgttgtccca ataccgaaaa atgcaatccc aatcgttgac
     3301 ataacagtaa aggcactccc tacagcgcta cctacgattg cacaaacaac aaatacagaa
     3361 gggacgaacc actggacact aatcaattta aatccaatga ccatgagggt tggaataatt
     3421 cctgcttgta tccatacagc aatcaatgaa ccgactaaaa taaaaataaa gattggaatg
     3481 atccccggtt tgatgccatt gatgatccct tcgtgcagcc aatctgtcgg gtattttttt
     3541 atcactgcgt acaacatgac cattccgata gctgccaaaa tcgtgatatt aggtgaaacc
     3601 tttaatccga caacgccaat cgcaatgcct aaaacaatga ccatcaatac aagtgttgct
     3661 tctaaaaaac ttggtgttcc ttttttttcc atgataatct ctcctttttt agttgttctt
     3721 atgttctaaa aaagttaaga tatcggatta ctccgccaca attcatccgc gattcctcct
     3781 ttataaaaat aaaaagacct gctttcaact aaggacgaat gactccgcgg taccacctta
     3841 attaaaaaca gatctgtttt tcactttcaa acgattattc aaatgtatgg actttccatg
     3901 taattcgtca attaggcttg atcggttcac agcgaccacc gactttctga acaaaacacc
     3961 taaccactac tgcgtgaaaa gttgacttgt atgaagttta ccttccataa gatcatttgt
     4021 caatgaaaaa gaaaatattt tttcattttt ctgaaaatta tttttataac tttcgcgcac
     4081 aactttatct ttttactaga aagtagcgcc ctcttgtttt tttcttgcta ctatttcttg
     4141 atgttcttgt ttgatttttt ctaatagctt gggttctctc aataaatcta gagctgtatt
     4201 ggcaagtaat tccgctgcaa tcgcaataga agcaagtcct ttttcacttt tagcagcagc
     4261 tttgaattct tctgtatgtc cggcaatata tgtctcagag attgctacag taggctgtat
     4321 cgttggaata acttggctaa catttcctac atcagaagaa cctagacttg ttctttgttc
     4381 cgtttctatt tcttcatcag gaacccctgc ttcttttgca tgttggaaga ataagtcatc
     4441 gaaagaaggc gttacgatga catcatcgac tgcattttgg aacaatccaa actcaaaagt
     4501 cgtacccgtg cttaatgcag cacctcgtac gatattttcc actttttcat atactttgtc
     4561 taatgtttgt cgatttcctg cacgtaaata gaaacgtcca gcagcgtagt ctggtatgac
     4621 attggccgcc acgcctccat ctgtaatgat tccatggata ttgacatctt ttggcaattg
     4681 caagcgtaga gcgttgattc cgttaaacac ttggatcaag gcttccaaag catttactcc
     4741 tttttcaggt gctgctgctg catgagaagc gacaccgtaa aatttgatat cgactggatc
     4801 atttgctaaa gattctgtcg ttttcccata acgaaaagct ggatggacac ataatgctgc
     4861 atcgacatcc tcaaaaaaac cttcccgaac gaagcttccc ttagctgatc cattttcgcc
     4921 tccttcttcc cccggagtac catacacacg aatttctccg ccaatctcat caatgaattg
     4981 tttcactatg ctggctgcta atacggagta agtaccaaat aaattatgtc cgcatgcatg
     5041 accaatacct ggtaaagcat caaattctgc caaaaaggca attgtcggac ctggtttatt
     5101 ggaaatataa cgtgcatcaa agcctgttcg gtgcccagct acttcttttt ctacccagaa
     5161 cccttctttt tccaattgtt tgattaaagc tgctgaggaa taaaattcat aattacttac
     5221 ttcaggatgg tcgtggatag ctagtgcaag tgcttgatat tctggtaaac ggtattgaat
     5281 cagatccttc aattgatttg ttgagagtgt ggttgtcata aaaatttcct cctatgctag
     5341 tcgttttttc ttattttctc ccgaatgttt cccacgcagg tacgcttgaa ccttttgatg
     5401 ttttttcgat tactttggct gtttcttctg tttgataggc atctactatt ttttgatacg
     5461 ttttattgtc ttcatcttct tttcttgcaa cgatgatatt gacataagga cgtgcatgct
     5521 catcgactgg ttctaagaag attgcatcag aagcgggatt aaatccagca tctacagcta
     5581 caccgctatt gatgactgaa atatctacat ctgacaaagc acgtgctgtt tgggaagcat
     5641 ctaactcaga aatgtctaga ttttttttat tctcggtaat atcagaaacg gtaggcgctt
     5701 ggccttttgc cttgtccact ttgatcaatc cagcagtttg caataacaga agagctctgc
     5761 cgccattcgt gacatcatta gggattgcaa cttttgctcc atctttgata tcagccacat
     5821 cttttaacgt attggaataa attcccaacg gtgcattaac cgtattccca atcgatacta
     5881 aatcagttcc gtgttcttca ttgtaattgt ctaaaaagaa ttgatgttgg aaggaattca
     5941 aatcgatcga tccatctgct aaagccgcat ttggctgatt gtaatctgta aatttcacat
     6001 attccaaatg gatgttttcc tttttcaaac ggtctttcac atcgtcccat acatctgtat
     6061 cttccccaat gatccctagt tttacggtcg tttctttttt ttcagttgta gctgcttttc
     6121 cttggctgca tcctgccaac caaataattg ctacaaatgt aatgactgca aaacgaataa
     6181 tttttttcat tttttctcct ccattttcaa tgtgtgactt ttttaatcag ttgattactg
     6241 ataaattgac tcaaaaaaac taagaccaga atcaataatg tagcgacaat cgttacatct
     6301 gtttggaatc gattatatcc tctcgatata gcaagattcc ctaatcctcc ggcacctact
     6361 gctccagcca tggtagtcag tccgatcaca ttgatgatcg tcaacgctga gacccgaatg
     6421 ataccaggta acccttctaa caaataaaca cgtaaaatga tgccgattgg gctcgtgccc
     6481 atcgcttctg ctgcttcgat caccccagga tcaacttcta gcagtgcatt ctcgatttga
     6541 cgggcaaaaa acgggacaac gccaaaaatc aaagggacta aagctgcttt ggcaccaatc
     6601 gtcgttccaa ctaagaaacg agtagtcaaa gctaaaagag aaagcaagat aatgaaaggg
     6661 atggaacgaa tgatattgat caacttatca agtatttcga acagcagcct gttagctagt
     6721 aatccatttg gacgtgtcac tactaacgtc acacctaaga tgattccgat gatcccacca
     6781 atgaatgccg tccaaaaaga catatatagt gtttgctttg tcgcttcgat gaattcatcc
     6841 ggaatcaaat agacattcgg caaatatgtt ttaaaaaatt cagtcatttt agtttcctcc
     6901 ttcgatgact ttgatacggt tatctaattc atttttttgt aaaatagtta cttttacacc
     6961 agatttaatc aaataatcca atgctttttg ccgctgtttg gtttctccag ctaaagtaac
     7021 gatcaaactt cctaagggtg tttcttgtaa tagctctaca ttaccgtata agatattcgc
     7081 tgttacttgg tacttgctgt aaagatgagc gattagaggt tcgttcgtct ggttgccgat
     7141 ataacttaat tccaccaacc atttatttgc aatctgatcg gcaaaagcag catgggcact
     7201 gattgtttcc aatgcttggt caaggtgtgt cgctgtccga ataaaatctt tagtcaattc
     7261 ttcttcagga tgactgaaaa tctggatgct gctacctttt tcaatgattt tccccgcttc
     7321 cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag tgatcaggac
     7381 gattgttaga cctagctgac gatttagttt ttttaacagt gcaaggatct gaagggtcgt
     7441 tttcggatca agtgcactag ttgcttcatc gcataacaac actttaggtt catttgccag
     7501 agctcgtgcg attgccactc gttgtttttg cccccccgac agttgtttag gatacgcaga
     7561 tgctttttct tccagaccga caagttccag tagttcattc actttttgac gacgttcctg
     7621 ttttgatttt ccagaatact ttagagaaaa atctacgttg tcaaaaatcg ttcgactttc
     7681 cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc gcaattcttt
     7741 tggagagagc tggcttaaat tcgtctgatt gacgatgact tctcctgctg ttggtttctg
     7801 caacaaattc atcacgcgga ctaacgtact ctttcctgca cctgagtaac cgacgatccc
     7861 ataaacatcc cctttttcaa tcgttaaatc tacagaatct actgcctgta tctctttttc
     7921 tttttgtttg aaacatacag aaatatctcg caattcaatc attaacattc ctccttgtct
     7981 ataaaaaaag catcctcaag aaaatgaaaa tagtcatttt cttgaggacg cctttttgcg
     8041 tgttaccacc tcatgttcaa gctgtgttca cacacaactc ctcttcaagt accatcatac
     8101 tctagcaaga tatcggtcgc agccgtcacc gccttacatc tatgctcggg atgaattgct
     8161 cgatggccat tttccaatag tcgttccttg cttcttctca gctaccgaag ctctctgtca
     8221 aaggttcttc tatcgtactt accatttcaa tgcaaataat attcaaaaaa aaaatccttt
     8281 tactaacata aactatgtca gtaaaaggac gacgtatcgt ggtaccacct tttttcggaa
     8341 gtttccttcc ctcaagccca acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg
     8401 aacatttccg aatttgccta cttatcgaca aattattctc caaggccatt ttcagctttt
     8461 cttacttgac tcttcacacc agccagagcc tctctgcaaa gtttccaagc ttactttcct
     8521 tctcaacgaa atcaatattt tgttgttggg aataatctac aggaggaaaa ctgaaaagtc
     8581 aagcatgaaa tccaaattat tttttctata tttatttttc tgttttttta tctaacattt
     8641 caataacagt tcgaagatct tttccttccg caagaaggtt caatttttcc ttctcttcga
     8701 cgctataatt tcgaacaaaa ctggaatcgg gaatcaaaac aacgcctagt cctgcacgtg
     8761 aagcggctgt cgctcctact aaagaatctt ccactgcaaa agctgaaaga gcttctaatc
     8821 ctgttcgttc caaagctgtc agataaggga ctggtgaagg cttgttctcc gatacttcat
     8881 ctccgaacgt aaacgtatca aagtaatcgg ctaattgaaa gtgatccaag atatctgttg
     8941 ctcttttcgt aacagttgtc gtagcaatcc ctagtttcat atgacgttct tttaagtctg
     9001 tcaatatttc ttttgcatag ggtttcaatt ggatagctcc acttgtcagc tgttgataaa
     9061 aatattcttc acgctttgct cggacttcct gcactttttc aggactgcct gctgcctgca
     9121 ctaaaatatc aaaactttgt ttccagtttt ttccgcccca agcgtcaacg atttctttcg
     9181 ggatatgaat atcaaattgc tggaatcccc aaagccaacc atcccgatac gttctttccg
     9241 tatccactaa caaaccatct aaatcaaata ttgcaccttt catctataca ttaatccttt
     9301 ccaaaacaat acacctagtt tagcatgact gatccaaaat gaacagaagc atagagccca
     9361 gccgcatcac ttataagagt tcttggaatc gacggatata gattttcttc acagctgtca
     9421 caagcgccag ataagcgacg actgttataa aaaaccataa ccagtaattg ccagggagcg
     9481 gcatcaagcc taactcagca ccaaatcctg tgaatggaag gactgaaccg acaatgattc
     9541 ctaatgtagt gataaccagc atggcaaatg ttgcattgct ttggataaat ggtattttcg
     9601 gtgtacgcaa tgcatggagt actaatgttt gtgtccacag ggattcgaca aaccatcctg
     9661 catggaataa tgcgataaaa atcacttgtt gtgagctatc caatgtatgg aaacttccac
     9721 caactacagc aggacaaata acaaaataca tcaacaagta agtagtgata tcaaaaatcg
     9781 aacttgttgg accaaaataa accatgaatt ttccaatact agatgcattc catttctttg
     9841 gctcatccaa atattcttta tccatccgat cccatggtaa ggaaatgcaa gaaacgtcat
     9901 agatcagatt caaaaataga agttgcaaag gcaacattgg taaaaatggt aagaacgtac
     9961 tggcaatcag tacggaaaac atattgccaa aattagaact tgctgttgcc ttgatatatt
    10021 tcatgatgtt tccaaaagtt tctcttccag aaagaaggcc tctttccaaa atcgtcaaat
    10081 ctttttctaa aaggatcacg tctgctgatt cttttgcgat atccacagct gtatccacgg
    10141 aaataccgat atcagccact ttcattgctg gagcatcatt gataccatct cctaaaaatc
    10201 cgacagtatg cccattttta cgtaaagctg tcgtcaatcg tgctttttgc tgaggattca
    10261 attttacgaa tacttcgtgt tcttcagcta cttgcgctaa ttcatgatca ttcatttccg
    10321 agattttctc accagtaatc aattcattga tttctaggcc tacttgacgg cacacagacc
    10381 gtgtgactaa ttcattgtcc ccagtcagaa cttttacagc tacaccatgt tcttttaaag
    10441 ctttcaacgc ttgttttgtt gtttcttttg gtggatcaag gaaagcaaga taaccaatca
    10501 gaaccatgtc tgactcatct ttgactgaaa attctccaac aacgcttgga ttggtctttt
    10561 gtgcgacacc aatcactcgt aagccgtctt cattaagtga ttgtactttt gcaagcaccg
    10621 cttcttttcg ttcttttgtt aatggtgcga cagtaccacc aagatcaata aatttagaaa
    10681 tgttcaacat ttcttcaatc gcgccttttg tgatcatctg tgttttgcca gccggatcag
    10741 cgactacaac actcatacgg cgacgttcaa aatcaaaagg aatttcatct actttttgat
    10801 aattgattgt atctgtatct aaagtttctg ctgcttcatc aatgatagcc acatctaaca
    10861 aatttttcag ccctgtttga tagtagctgt ttagataagc atggcgcaaa acacgatcat
    10921 cttcatttcc ttcacaatcc atatgatatt caagaatgat tttgtcttgt gtcaatgttc
    10981 ccgttttatc cgtacataac acatcgatcg caccaaaatt ctggatagca tttaaatttt
    11041 tgatgactgt tccttttttg gccatcacac ttgctccttt gaccaaattc gtcgtaacga
    11101 tcattggaag catttcaggc gttaaaccaa ctgccacaga taatccaaaa agaaaagctt
    11161 ctaaccaatc tccttttgta agcccattga taacgataac tgctggagcc atcaatgaca
    11221 taaatttgat cagcagcatg gatgttttgt ggatgcctaa ttcaaaactt gattggatgg
    11281 gtttttcaga tagcgtttct gctacatgcc caaatagtgt gtcatttccg acagaaacaa
    11341 tgattccttc cgcgcttcca ctgatgacat tgcttcccat aaagacaaga ttttcataac
    11401 ttgtttcact ttgctgatcg tcttcacata ctttcgcatg cttttctact ggatagcttt
    11461 cccctgtcaa tgctgcttga gaaatgaata gatcctttgc ggacaataaa cgcatatccg
    11521 ctggtatcat atctcctgct gaaagccgaa caagatctcc acagacgatt tcttccatag
    11581 gaatctctgt atactcgcca tttcgtttga cagctgccgt tactttcaca agtgatttca
    11641 gtttttcagc tgcttgatta gaacgcacag actggatcaa cgtcatcgtc ccactgacga
    11701 taaccatagt gaagataatg gctacaccaa agagatcttt atctcctgga gcggctaata
    11761 cgtattccgt aatgaatgaa ataattccta atgcaatcaa cactaatgta aagggtgtga
    11821 tgtatgcttt caatatttct ataatgaatg gtgttttttt gccatatgaa atcgcatttt
    11881 caccataagt ttctcttgaa cgctcgattt gttcttctga caatccattt ggtgatgcat
    11941 ggaacaattc aaataattct tgcttcgttt ttccagcaaa agctgcatag attttttgtg
    12001 tatctttctt tttttccatc tcgatctctc ctttgtgtcc aaaagacagc tgttctcgct
    12061 gtttttggtc ttttgctatt catcggatcg atagagaaaa tgctgaaagg ggctgagaac
    12121 agatagataa tagccctggc ctttcctcta aatccttgtt tttcgaatga ctgtcatcca
    12181 tttgttctca tctcctttac taagtaattt taattcatga cttccaatca tttttcgaat
    12241 agttattttc ccaactcgct cacctccctt tttgtttttt cacctatggg atttgcggca
    12301 acaaaaaaac ctctcgccaa atactcagcg aaaggtttga aaaagcaaat caaaaaaagc
    12361 ttctcagatc tcatcgttga gttttgacac tgtacaacgt aaaaagcaga tatctgcttt
    12421 tagctacctt atgaaaagcc ttatctcgac aattcctgtt ctacccattg gcgtctctcg
    12481 acgtttttgg gcagtagcct atgtttgtat aggagcctca cctaacaaat cctattcggt
    12541 tttccctcgt taatataaca actaaaaaag aggatgtcaa atcaattttc catgtttttc
    12601 atactttttt aaaatacacg cgttatattt cctgtctcac gcagttttat ttgtctcatt
    12661 ttccacctcc cgctataata gaattatagt aggaaacgag gtgctgttat ggaacgaaca
    12721 cttgttatta tcaagccaga cggcgtaaga cgccatttgg tcggaagcat catccaacgt
    12781 tttgaagcaa agggactggc cattgctgaa atgaaatttg caacgatgac acctgaacta
    12841 gcaaaagaac attatcagca cttgactgaa cgtagttttt ttgatgagct tatcgattac
    12901 atgacttctg gtccggtcgt ttatcttgtg ctggtcggcg aggaagtgat cgatatcgtc
    12961 cgtaagatgg tcggagcaac aaaaggggcg gatgccgttc caggtacgat ccgaggagat
    13021 tatgcactgc cggggactga aaacatcatc catgcttctg attcccgtga tgctgcaaca
    13081 aaagagatcg cccgcttttt cccgccattc gatacaaaca aaccgtctga aaaagcggcg
    13141 aagtgataca aatcctaaac tgttggtcta actgctgggt ttagcaatgg ccagcagttt
    13201 tttctctaaa gaattccttt gattcttccc taatgtatat tcgatcactg cgaaaaactc
    13261 ttggtgattt tccgattttt cttcccctaa agcagcagaa atcagtcgtt ggtaataatc
    13321 cgccatcaat gtttcataaa tcactgtact tctttcttta tgcgctaaac tccgtttcat
    13381 atcttcattc acgctacgtg ccttttttaa gcgaccattt tctatataaa acaaagtcaa
    13441 gttcgtcaaa aaagtaaaga gatactgctc gtgattattc agttgcgcga tccgtcgcat
    13501 tctttttttg attttcaaat aattattctc aataaaacga tcactgaata tccaaggaaa
    13561 atctgctaga atagatactt caaattgccc atactctggt acttgcatca aatattcttc
    13621 aatgatacga cattcataac gcaaaaattc aaattccgtc tcagatactt ctgttttttt
    13681 ggacatgtgc agattcaaaa ccaaatagta cagatcttct gttttcttat agtgcctgcg
    13741 gttattagca atttctttgt aaggatcaaa gctgtcttct tgaatccgat ctagcattcg
    13801 tctttttttc tttaattttt ttggctcatc aagctgattt cgtatccatt catattcggc
    13861 aagtgtcatg ttcattcgct ctaaaaatga tttcaatgtt ccaaacgata cttttttcac
    13921 tgaatttgtc tcgatcccga ctagcgaagt tttagaacag attccttccg ctaattgggt
    13981 ctgtgtcagc tttttgttga ctcgaagctt cttaatcaat tctccatcat acataatagt
    14041 tctccttttt taattgaaat taacccttgg cttttccaga atgcttgcca ataagaggaa
    14101 tgattatttc aatttaaatt ttccgcattc aataaataga tacgttaaaa ataaaaaata
    14161 cacgaaaaaa ataaaaaaat gtttttctca taaaaaatac caaccttcaa gcatgagact
    14221 gctgtctaat ccctgaaggt tggtttgatt gtttattgat cattttttta ctgctccgca
    14281 ttggttcctg ttactggata taggatagtc gttgttttat tgccgttttt tgaaaagaca
    14341 ctggtcgaat gttcaccttt ttgcttttcg attgcttcat tacgttccgc ttcgttttta
    14401 tagtcatatt ttttcggatc aatcgtctca aatccgtttg gcacataaaa ccgcaagaga
    14461 ttttcctgat tcagcttatc tgaaagactt aacgcagtct tgactttttc ctgatctttg
    14521 gagatttcct cgtcgattcc tgctgctttc gtatcgatga tttctcctgt ctgattattg
    14581 taagcttttc ctcctaggac ggtgtatttg ggcgtcacga agttcccatt tcgaaaagca
    14641 actacttgat cgtgctgaga agataataag tctgtaccaa aatgaagata atctttatca
    14701 tcgattccca atagatgtag taatgtcggc aaaacatcga tttctccacc atattcatga
    14761 ttgactgtcc ctttagtgta accaggcata tggatcatca atggcactcg ttgcatctgt
    14821 gcgttgtcaa attcatccca tgtatctgga tctttaccaa gagcacttgc taaatcttta
    14881 ttatctgtat tcgagatccc aaaatgatcc ccgtaaatca caaagattga attttggtaa
    14941 ataccagatt ttttcaaata cgtaaaaaat tgttccaatg attgatccag ataatgggcc
    15001 gttcgaacat aattatctac tgtgctattg ccagtattca aagatggaaa gtcgaaattc
    15061 ttgtcatccg tataataagg cgtgtggttg gttacggaaa gaaatttcgt gtaaaacggc
    15121 tgttgtagat gttcaagata ttgggcagat tctctgaata aatctttatc taatatccca
    15181 taacctaacg tttcatcgga ttcatcaaaa taagatcgat caaagaaatt gtcataccct
    15241 aaatttttat atacatgatc tctattccag aaactagcta cattcccatg gaaaacagca
    15301 cttgtatagc cttctgtttg accaagggtc gctggtgctg cttgaaagac attatctgac
    15361 ccaagttctg tgaataatga gccttgaggt aaaccaaatg tgccagtttc caacatgttt
    15421 tcagcatcac ttgtctttcc ctgtcctact tcatgaaaga aattgtcaaa actgatcgtt
    15481 gcttgattct ggaaaatact atttaaaaac ggcgtgactt cttgtccgtc taccttcata
    15541 ttgatcaaaa actgttgaaa actttctaaa tgaaggacaa tcacattttt tcctttagca
    15601 atcccaaatg tttcaggatt tggctctgca taatgtttct ttgtatagtc taaaacttca
    15661 tcaattccat tactgcttgc atgcgcgcga acactgctag tcattcctgt tttgattccg
    15721 tcataaatag tgtacgcatc gattcctaga tatttgacaa tataggaacg gtcaaatgtc
    15781 cgctgtagca attggggacg gttggcttcg ctcaacgcta aatttcctga aaaaatcaat
    15841 cctgaaagac aggtaacagc gatagccatc ggtttgctga cagggttgct tttgataggt
    15901 attttcttga ccttcaacca aaccagtagc ccaatgaaca cagcgatatc caaccaatag
    15961 atgacatcat ggggtttcat caaagaaaag gagcttcctg acaacccttg tgacactttc
    16021 gaaaagccta atacagattt gactgtaatg aaatctgtga attcccgata aaaaataata
    16081 ttcaagtaca acaaaagcgt atttaaaata tcaatgatca aaagaatggc taacgctggc
    16141 tttggctttt taatatacaa agaaaggcca aaaaacaata aggtagtcgc aatcgggttg
    16201 atccacaaga taaagtactg gatcgtacct tccacaccta atgaaaaatc tgcatagtag
    16261 gcaatgatcg tcttcagcca gaaaaagaaa acagctgtca aaacgattgc taaacgattt
    16321 tggcacaatt ttttaagttg tgtcacgtat atcacttctc tctattcaca ttttttctta
    16381 ttttgctcct gaaaaatgaa aatttcctaa aaaagttgtt atcttaagat ttttttattc
    16441 tttctaaaga ttattccatt catttttcac agcataaaat cgtttcggtg taagcggtta
    16501 aaatagtaag gagataaaaa tcttgtggtg accgatttac tctatagtat tcaaaagtag
    16561 tttctcggta ttcgtacaaa aaaaacatac taagattaga ggggaacatc attgacgaat
    16621 gatgttctcc tctattttct atttttagat tatagtgaag agtgaaagaa agttcgaaca
    16681 aagccttgag gactacgagt ccctatcaaa aaccgaactt ctttactgtc attttagaat
    16741 caggtttacg tatgttgggg ccatgagccc gtgtatagga aattggaatc gaaatgaagc
    16801 agtaaagttc tttcaacact taaaaaggag gaaaacaaat gccttacatt cttgcattag
    16861 atgtttcaat gggacacagc tacagtgtcc tatacaaaga cgacacctgc ttatttgaag
    16921 acgagatcga acacactcaa agaggatttc atgcattact cgaagagatc cagcacttgc
    16981 ctgaacgtcc attgattgtt tttgaagcaa cgggtattta ttccagacca gtcgaaaagt
    17041 tctttcaaga tcatcacttt tcttactgtt tgttaaaccc gttagaagcg aaaaaacaga
    17101 tggaagaaaa caccttacgc agttggaaaa cagataaatc ggatgcccat cggctcgcac
    17161 agactcatct taaaaatcaa cgtcaaccaa aagaagtaca aagcaatttt tacttagaga
    17221 tgcgcgattt agcccgtttt tatcaagaaa ttgagaagaa aatcactcga ctgcgcatgg
    17281 atttacacaa ctgccttcag ttgacttttc cagagcttga acagttcttt tcaaacaggc
    17341 tgacccctta cgcattgaca ttgatccgtc tttttcccca tccagatttt gtattggcat
    17401 ctactcggac aaaaataaaa aataaactga tcaacgaaac acgggaaaaa atctccgcga
    17461 atcgtgccga gcaaaaggca gatcaaatca tccactatgc acagtgtgcc tatccagctg
    17521 ttgaaaaaga cagtattcac tgtcaaaaaa cgatctatta cgccgaactt ctccaagatt
    17581 tactcgaaca aaaaaaagca ctggcgacac aaatgatcaa aaaagccgaa gcctctcctt
    17641 gctttttgtt gtaccaaacg ttccctggga ttggcgcact cacagccgct ttattgctcg
    17701 gtgaattagg agatatcaca cgatttaaaa cccataaaca attgaatgcc tttgtcggaa
    17761 tcgacatccg acggtatcat tcaggtaagt atactggcca agaccgcatc aataagcgtg
    17821 gtaatcccaa agcacggaaa attattttct tcaccattcg aaatatgatt cgccagcaac
    17881 gcgcagctcc taatcatatt gtcgattatt actacaaatt aaaaaagcaa cctatcccta
    17941 agaaagataa ggttgccaca gttgcttgta tgaacaagct tctgaaatgt atgcacgcta
    18001 tggttagggc acatacagag tacgactatg cgtacgcggt ctcagtggac cattaactaa
    18061 accacttaca gtataccact tctttgccca gatcgaaaat tttttacgaa cggggcttta
    18121 tttagtgtgc cttgattttg tcgctttcgt ttacgagcta tttttcttgc accttttact
    18181 tgactaatcg taggaaagag gctgtgacaa aattcttgtc gcagctccct atttcttgat
    18241 gcaggacagc tttttcacaa cctctccact aaacggtgtt caatcagctg accttcggta
    18301 ttagctcaaa aaacggcaaa atatgaggag ctattttccc gtttttttct ctaatactca
    18361 ggtcaaaccg ctgatttcac aacctctgat tcacggtgtt ccagaagtag cttcttcgga
    18421 aataagccga aattttacaa aaacttgaga agcacttttc gtaaatttct tcttatttct
    18481 tgaagctgac cacttctgtc acaacttctg tttttcattg tccataggtt tgatattgtc
    18541 cttctgatga ctcattcgta atgattgagg cttgattttc ttcatttccc gttatcgttt
    18601 gtccactgcc agaatctgaa gggcttagtt ctgtcgactc gtttttttga gaagaagcag
    18661 ttgttttctt gacttgttcg ttggtctttt tccattccgc tagtcgatcg gccaccactt
    18721 ttttgtccac tttttcagaa taagctgtaa ccacttttcc atttttcaaa cgtgctacct
    18781 tgtcgccatc atgagtcaaa agcaaaacga ccaaatgttt gtctttgaat tgatattgtc
    18841 tgagagaaat cgcactaagt acgtcatcga tattgataaa ttgattttct tcttttcctt
    18901 ttggcaaata gatgtccaat tcaccttttt cgtaattggc agttaattta tattcctccc
    18961 accaataagt tgagaattct tttttgaaat tatctactga ttctttgctt gctacgattt
    19021 tttctgtttc tttctgagca agatctgctg tcatttttcc tgaaaatgct gcggataaaa
    19081 taagcagtaa gaaaaaaccg aataaccaaa agagaagaaa cggtcttttt ttcctcatgc
    19141 cttcctccac tcctatttaa ttttgactat tctattatat acaaaaaatg aaatagaaag
    19201 acaaaaaagt tgaaaaagat cgtaaataat tgaacttctt tttcctgaag cggtaagaac
    19261 gggaattttc atacattctc tcttggctat tctttaagct tcaaatccat ttctgtctga
    19321 tacatctttg aaccaatgac ccgatttctt gatcgttctt tttccatctt gtgctaaatc
    19381 tactgaaata aaaccgtaac gattcttata ggcattggtc cacgaccagt tatccatgca
    19441 tgtccacata tgataaccta aacaattggc accttcttga atggcttgat ggacatattt
    19501 cagatgagat tgaacaaatt cgatccgata atcatcgtgg atcataccat cttccttgat
    19561 aaatcgtgct tcattttcta cacccatgcc gttttcagaa ataaaacagt cgatattccc
    19621 ataatgatct cgtacattga tcaaaatatc atagattcct ttttcataga tctcccatcc
    19681 gcgataagga ttcattttac gaccaggcat ttcataattg tcgaagtaat cctcaggcat
    19741 aggaccgttt ttagtatcga ttggtgtttc ttttgctttt attcggcgtg gttggtaata
    19801 attaattccc aaaagatcta ctgtgttttc tttaatgatc gtcaaatcat ctggatgaat
    19861 ctccggcatt agatcgagtt cttttattct ctcaatcaat tcactgggaa actcgccttt
    19921 tacagctgga tcaaggaatg aacgattgaa gaacgcatct gctattcgtg ctgctttaac
    19981 atcttcggga tttgattcgt ctcttggata actcggtgtc aaatttaaga tgattccgat
    20041 cacaccgctt tgatccattt catggtaagc tttgatggct aatgctgaag ccaacgcttc
    20101 atgataacca acttggattg cctttttcat atctgattct tccggataat gccatcgata
    20161 caaatagcct gcctcgactg gaacgattgg ttcattatgg gtaaaccatt tttttacttt
    20221 cgagccaaac aattgaaaac aactttttgc gtatttttca taggcttcaa ccgtttctcg
    20281 gttcaaccat ccgccttttt cttgcagata catcggcata tcgaaatgat acagattaac
    20341 aaagggttcg ataccgtttt caatcaactc atcaaaatag tgatgataga aacgaacggc
    20401 ttcttcgttg attgttccag attcatcagg tatcaaacgg ctccattgga tagaggttcg
    20461 aaatgaatta tgcccagtct gtttcatcaa ctgtacatcc tcttgatact tcgtataaac
    20521 ttgcgaggtt ttttcaggtc ctacttgatc aaagaacttt tgcggttcgt ttttgtacca
    20581 ataatcaaaa atattctctc cttttccatc gcctgaaatt cttccttctg tttgtggacc
    20641 acttgctgca gagccccacc aaaaattttt tggaaattga tatcttttca tcaaagcacc
    20701 tctttttctt ttttagttga ttccatgtat gatattttcc atcacaatca ctttttcatt
    20761 ttcttcctct tcttttttga ttcgtttcaa cagctgttct gctgctcgtt ccccgatttt
    20821 atctgttggt ggcgtgatcg tgatttttgg aaggatactc agatcgtttt tatctaaata
    20881 atcatagtga ctgaacgcaa tatccttccc gattctgaca ccttcttgat tgaaacattc
    20941 gatggcacca atcgacatgt tgtaattgct gacaaaaata gcagttgccc ctttttctaa
    21001 taatgctttt gccccgtaat agccgctttt tctagaatag tctcctgtaa agatctctaa
    21061 atggatagac ggatcctttt gtaccgcttc tttggctcca gctaaacgtt ctcttgctac
    21121 gtaatctgtt tgagcggcag caataaatcc tattgtttta tgtccttgtc cgccatgatg
    21181 agcaatcact tttgctacac tttctcgatc attcactact attgtatcaa tacctgctgg
    21241 cccacccgga caattcaatg cgacaactgg gatactcatt tttgtcagca tttcaaatcc
    21301 agaccactct tcttctgata agaagacgat caatccatcg atcgcatgct tcatcaggta
    21361 ttcgattttt tgttcgattt tttctggctg atcggcaaac ccgcttaata ataagctata
    21421 cccattttct tcagctactc tttcgatccc tgaaacaacc tccgaaccaa atcggttaga
    21481 tatcccgttt atcaacagtc caatagagaa acttcgatta ttcttcaatc ctttagcaat
    21541 gatgttttct ttatagtcca acgctgaaat cgcttctgat attttttcca tattcttttc
    21601 cttcagctgc tgaccgttta aatagcggga aactgttccc actgaaacac cagccatctt
    21661 agccacgtca cggatcgatg ccatacattt tcactcctgc cagttacatt tgatttcttt
    21721 ttcttgttgt tcgaccatga tgaccgcttt ctttgcataa tgccgcttgt catcaatatc
    21781 catataacta aagtcttcag aagtgagttc aaaaacaagc agtctttcct cttgagaccg
    21841 caattcatct ttacgaaaag ctttcaataa tttcgttctt tggatgacaa cgccgtcaat
    21901 taatcggaca tagacaagag tacttgtttt acctgaaaca cttcctgtat ttttaacttt
    21961 aattgatatt attactttgt caatatccgt tttgacttcc atctgactgt attcaaatgt
    22021 tgtatagctt agaccatgtc caaaaggata aaatggactt ccgcttaaat cataataatt
    22081 ctcttgttta gaagcagcac gctgatagta ataaacaggt agttgccgac tatttctagg
    22141 ataagacaaa ctcaatcgtc cgcttgggtt atttcttcca gaaatcatct gcgcaatggc
    22201 ttttccaccc tcctgtccag gaaaccatgc tgttgcaatg gcattcgtaa atgactcgat
    22261 tttcatcaaa tcgtagggtc ttccttgcac aagtatcgtg atgattggct tgcctagttg
    22321 atacaattgt tcaatcagct gttcttggta tccacctaaa gaaagagaag caacatctac
    22381 attttctcca gaatccatgt tgatcccttt cgatgaaact gccccgttac gaagaaattc
    22441 catatcaaag ttccgcgcac ttgatccacc tagaactgca atgatcacat ctgagttttg
    22501 agcaagggtc accgcttcta ataatttttc ttcctgatcc tcctgatttc ttacttcaca
    22561 tccttgtgca taagaaacct ttgattgggg aaactctgtt tttatctctt gtactatctt
    22621 ttttttcaaa cggtcttctg tttgtggtgc agagtagtct cccaacaagt ggtaaacatc
    22681 gtgagcattt ggacctataa cagcaatgtt ttttacttgt tgatccaacg gcaatatacc
    22741 gtcatttttt agtaaagtga tactttcttt tgctgtctct aaatttaagt gctctgattc
    22801 ctccatgaga tgatcccaat actcctttgg atcgtttgca aatggctgtt caaataaacc
    22861 taataaaaat ttgacagata aaactcggcg aactgcttga tctagtagct tttcatctac
    22921 gacttgatta tcaatggctt ctgcaatctt ggtataggtc tcatcccaca aactgagatc
    22981 gattcctgcc gctaatgcat atgctgcagc agtcttttta tctgtaaaaa catcggataa
    23041 acgatccaaa gccacaccgt ctgccataac gattccttga taaccgatct cttttcttaa
    23101 tgtttgctca aaaagcgctc gattgatatg acaaggtatc ccgtcgatgt cattataagc
    23161 agccatgaca cctactgcat ttcttgtact cttcaataat ggtccgtaaa tatcggagaa
    23221 ctctctgcgt ccaatcgtta ctgtgcctga attatgaccg cctaaagctt ctccttgtgc
    23281 gatacagtgc tttaacacaa caccgatctg atccttcttc ttattgattt cctctactgt
    23341 ctgatcaaga aaagaaactt tttctgaaat aatctctcct tggaaccctc gaacgaccgc
    23401 ttcagaaaaa cgagctgaga gaatcggatc ttccccaaag cactcttctg tccttcccca
    23461 acgaggatct tttgccagat ccaaagtcga caccagcgct aaatgaacac ctttagcagc
    23521 cagttctttt gccatcaatc gactcgattg ttctaataat gctacattga aagtatttcc
    23581 acggccgata ttcgttggat aagaaatccc atccaagcct tgatgaccat gtggacattc
    23641 ttctacaagc aaaactggaa ttttccatcg cgaatgctgt agtacatatt cttggactag
    23701 gttagccacc ttccagcttt cttttgcagg aatcccattc ttataatctg ttttagacca
    23761 cggatcagag cgaaacagac cgtataaggc acccagaccg cctccccatt taacatgttc
    23821 ttttaacttt tctgttaatt cgatcttgtc tccgtttttt tcataacatt cccaaccata
    23881 caaatgctgg tttacctgtc ctactttttc agcaatattc atcctcgaaa gtaaatcttc
    23941 tactctttgg cggatggtta actttgggtt ttgatacggg taatcgtaca ccttaatatc
    24001 ctccttctgc caattttgcc ttattagcgg ctaatacaaa tggcaggaat aaaaatacat
    24061 ctagaatgat aagtattact gttaagactg ctgctcgtat atcgcctcct gtagcaataa
    24121 aagattgtat aaccggtgga gtgatccaag gtgcagcagc aactgtttta ctgatgattc
    24181 caagacttgt ggctgcgtag gcaataacca agttaataga tggtataata gcgcatggaa
    24241 tcatatatat tggattcatg actactggta atccaaacat aattggttcc gtaatattaa
    24301 ataaacaagt agtcagacca aatttagcga ttgatcgctg ttcttgacgt ttagaagccc
    24361 acaagatagc aataactaac ggtaaaatac acccgccacc tccgatcaat ccaaatgcat
    24421 tggtaaaggg ctccgtaata atattaggaa tttcttttcc tactgaaaaa gcatccatgt
    24481 tttcctgaat agactgtaac aaaggtgctt gccgtatagg agccaaaata ctcgaaccat
    24541 gcataccaaa cacccataag aaatcagaga aaaactgtaa aaataagatg cctgggacgg
    24601 ataaaacaaa agctttcaat ggtgtctgaa tcgctaattc aattaattgc ggaacattca
    24661 aaccagtcgt atggtttaaa gtaaaaacga caactgaaaa aaataaaatg acaactattt
    24721 ctggtactaa agaattaaat gacgcagcta ctgccggtgg aactccttct ggcatcctga
    24781 ttttaaaact cttcattttc gctaatttga taaataattc tgtactgaca agggcagcaa
    24841 tcatcgccgt tgctaagcca gacgaagaag tcagactttg taagaaaacc cctgtagctt
    24901 ctgctgtatt tccgtccatc aaagtgactg tacttgataa agacatcaaa ataaacatta
    24961 cggctgtact tagaattcct gcatgaacag gactaaatga tggatcatca atcacgcctg
    25021 tttggatata gtgggttgcc aaattccagc cgatattgta acacacgata atagctaaaa
    25081 tactcatcgt accattattc acgattgaag caagttcagc caatggactt agatcaagta
    25141 ttctttggac tgttgcattt gaaaaaatca gagcattcag caaaaccatg acagaagcag
    25201 ccattactaa cggcatagta acgataaaac tgtctcgaat ggccagcaag tgtcgctggc
    25261 tactcatctt agatacgatt ggtagcattt tttcagatag tctgtctacg actttcatta
    25321 tttcatctcc tagattctaa aattgaaccg gttcattttt aatatagcac tttttttaaa
    25381 gattgcaaca cattttttta aaacgctttc taaaggtggt ggatttttta tgacttttga
    25441 aaaattattc tatttaatgg tattttgtga attttttctc ttccatccgt ttgaagtttt
    25501 actttccaat ggtataattt ctttatccta tcaaatgggg gaagtatttt gaagaaatta
    25561 atcgtactat ttattttgtc tgcgggactt ttgacattga ctggctgtga tttgtttggc
    25621 ggctctgacc aatcctcgga cgaaacaacc gattcttctg tcttaaaagc ggaaaaagca
    25681 gatatcacca acagtgtata tacgctaaaa gtacgtaaag atatggattt atccgcggga
    25741 cagttgacat ttacagataa aaaaatggaa tggacacgca cgtatcagac acaaggagct
    25801 gattcttctg aatcatctga agaaaaaaca gtgctgacag atatcaagat tactaccaaa
    25861 gatgacgaat atattatttc aggaaaagaa aataaaaaag aagcaacagt cacatttacc
    25921 aaaatcggta attaccgttt gaaggataag gatggcaaca tctacagtct atagtgccat
    25981 ctgttggatc ttcttttgtt tctccccttc ttttctgcta tagtaaagaa gcaagatgac
    26041 tgtaaaggag acgaaatgga tgatcgatag tcctgattat tgtacatgta ataacaacaa
    26101 aaatatccat acagaattta atgatcaagg aagtttgagt atatgcgaca attgccacaa
    26161 actagtcaaa gattctttta aatcgtttga atcttatcct gtcaaaaatt atgtaaaaag
    26221 caaaagaaga taagcaaaat caagcaaaac tcactatggc tgatttttat gaacttcaaa
    26281 ctcagcttct tcctttgatc aagcttcaaa aactggaatc agtaaaacaa acagtggatc
    26341 atttcagttc acaaatcaaa gagtaacgtt ttatttattt cacatctttg aatataaaat
    26401 aaccaaacga tagaaagact tattctacat cacgtagtaa tttttctttc ccgtcgtttg
    26461 gttttttact tttagcctaa ttcttgacct ttcgcatgct gattatctgt aaaatatttt
    26521 ccagcaggct ttccttccaa ttcagctaat ttttcttgga tctgacgaat cacctttttt
    26581 gcatcttctg gattttctac aaaatcgaat gtatccccat ccacttgtat tttaggacat
    26641 acatcaaaat cctcatacca ttgattatag cgggtgttca gtgtttcgta ataggtgtat
    26701 aacgtttcat cgctttctag ctgttcatag tcacgcccgc gttttttgat acgttctagc
    26761 atcgtgtcca aagaaacttt gatatggacc atcaaatcag gacgtttttt tggagcaaca
    26821 tcatctaact cgtttagcat cgtatctaat aagctatcgt actgctgtac ttcgatatcc
    26881 gttacccgac ctaaatcagc atttagatgg aacaacagac tatcttcgta gatggagcgg
    26941 tctagcacat tgtcatcgtt cactaaagca tttttcattg ctaaaaatcg cttattcaaa
    27001 aagaagattt gcaataaaaa tgcgtactgc tcaggatttg aataaaaaag cggcaatact
    27061 tcgttatctt cgacattttc ataaaatggt ttagaatcaa tctcttttgc aagcatatcc
    27121 gtcaaactgc tcttacctgc accaatcgtt cctgaaatca aaatcactga catacaaccc
    27181 ttcctcttct ataagtactc gcctacgtaa gtttcaacag gcaatttttc aaaatcatat
    27241 tcacgtacag cttggtctga ttttaaatac gtgtgaagac tttgtgtaat catcaagttc
    27301 gtaggtaccg tctcttcttg tacgatgatc atcggttttt tcaacatagt tgccacacct
    27361 aattcgtaag ccgttcctgg atcgattgtt tgatgctcaa agtcaaggat agccaaaaca
    27421 aattctgcat ttgtcaactc ttccatatct ttttcgtata cttcctgcgc ccatccagcg
    27481 ctgaatagtt cataattgct ttcttggtga tgacgtggag agtagaaact tgtgattgtt
    27541 ttgttttcct ccaacgcttt ttcgatacga cttactcgat cgatttgctc ctctgaaaaa
    27601 aatggtcctg ctaaatatat ttgactcatt attttgcctc cttcaataat aactgatttt
    27661 taatagatac tgctcgtaat tcattgacca gttccaatgg attgttggaa aagatcactt
    27721 gatccaacca ttttttatcc ataaaatcct cttttgcaaa agtttctaat aattgttgca
    27781 agccgctgta ataaccgcca atattcaata ttccgattgg cttatcaggt gtcaggccga
    27841 tcgctatcca agaaagaagt tgcgaaaatt cctccaatgt acctagacct ccagggaaaa
    27901 cgaggacagc atctcctttt tcaaataata atacctttct ttcatcaatt gtctctgtag
    27961 gaataaatgt agtaacatct ttcccaggca gctcagtttc aaaaaggcca tttggataga
    28021 tacctgtgac ttttccgtta ttttccagta ctgttttact aatcactccc ataatcccgg
    28081 agtatgaacc accgtaaacc agttcgattt cttcttgtgc catatatttt cctaacgttt
    28141 gcgcaataaa tttgtaactt tctttattgc caaaacggga accgcaaaaa acagtaagtt
    28201 tcataaaaaa acactcccta actcaaaagt ctatcttcat tctacacgtg tttttttaaa
    28261 ataaaagact agattttgta gttttttagt ggtat
//
LOCUS       DACESX010000024        28226 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.024, whole genome shotgun sequence.
ACCESSION   DACESX010000024 DACESX010000000
VERSION     DACESX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.024"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            41..277
                     /locus_tag="IXF88_000898"
     CDS             41..277
                     /locus_tag="IXF88_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1507643.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            274..672
                     /locus_tag="IXF88_000899"
     CDS             274..672
                     /locus_tag="IXF88_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1507644.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(1419..1697)
                     /gene="ldh"
                     /locus_tag="IXF88_000900"
                     /pseudo
     CDS             complement(1419..1697)
                     /gene="ldh"
                     /locus_tag="IXF88_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Converts (S)-lactate and NAD(+) to pyruvate and
                     NADH; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
     gene            complement(1993..2937)
                     /locus_tag="IXF88_000901"
     CDS             complement(1993..2937)
                     /locus_tag="IXF88_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1507645.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            3158..4371
                     /locus_tag="IXF88_000902"
                     /pseudo
     CDS             3158..4371
                     /locus_tag="IXF88_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4393..6408
                     /locus_tag="IXF88_000903"
     CDS             4393..6408
                     /locus_tag="IXF88_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1507646.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6491..7864)
                     /locus_tag="IXF88_000904"
     CDS             complement(6491..7864)
                     /locus_tag="IXF88_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1507647.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7890..8174)
                     /locus_tag="IXF88_000905"
     CDS             complement(7890..8174)
                     /locus_tag="IXF88_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1507648.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(8176..10191)
                     /locus_tag="IXF88_000906"
     CDS             complement(8176..10191)
                     /locus_tag="IXF88_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1507649.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10370..10675)
                     /locus_tag="IXF88_000907"
     CDS             complement(10370..10675)
                     /locus_tag="IXF88_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1507650.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10764..11795)
                     /locus_tag="IXF88_000908"
     CDS             complement(10764..11795)
                     /locus_tag="IXF88_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1507651.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            12002..12877
                     /locus_tag="IXF88_000909"
     CDS             12002..12877
                     /locus_tag="IXF88_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1507652.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(13029..13871)
                     /locus_tag="IXF88_000910"
     CDS             complement(13029..13871)
                     /locus_tag="IXF88_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1507653.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13963..15840)
                     /locus_tag="IXF88_000911"
     CDS             complement(13963..15840)
                     /locus_tag="IXF88_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1507654.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15837..17258)
                     /locus_tag="IXF88_000912"
     CDS             complement(15837..17258)
                     /locus_tag="IXF88_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1507655.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(17261..20650)
                     /locus_tag="IXF88_000913"
     CDS             complement(17261..20650)
                     /locus_tag="IXF88_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1507656.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20907..22298
                     /locus_tag="IXF88_000914"
     CDS             20907..22298
                     /locus_tag="IXF88_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1507657.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22371..23018)
                     /locus_tag="IXF88_000915"
     CDS             complement(22371..23018)
                     /locus_tag="IXF88_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1507658.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(23026..23712)
                     /locus_tag="IXF88_000916"
     CDS             complement(23026..23712)
                     /locus_tag="IXF88_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507659.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23718..24791)
                     /locus_tag="IXF88_000917"
     CDS             complement(23718..24791)
                     /locus_tag="IXF88_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1507660.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            25013..25687
                     /locus_tag="IXF88_000918"
     CDS             25013..25687
                     /locus_tag="IXF88_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1507661.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25692..27032
                     /locus_tag="IXF88_000919"
     CDS             25692..27032
                     /locus_tag="IXF88_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1507662.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(27181..28167)
                     /locus_tag="IXF88_000920"
     CDS             complement(27181..28167)
                     /locus_tag="IXF88_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1507663.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8305 a         5267 c         5109 g         9545 t
ORIGIN      
        1 acttactata gaatgagaat ggtatagagg gtggcgaaat atgaaaaaaa tgaaaaagta
       61 taaaattaga aaatctggca atagcgatgt aacaactatc ccaccagaag taaaagaatt
      121 catgggtgtg cagacaggtg acgcaatcag ttatgttttt caatctgatg gttctgtacg
      181 aatgatcaaa gcacaagaag aacctgatat tgattcatta gttgattcga tcatgaatca
      241 atatgaagac gcgttaaaag atttggttga tttatgaggt atctttcgac taaagaaatt
      301 atcaaaatca acgcgaaagt tattttacgt cactcgccag gcgaaatgat cggtgtaaaa
      361 gatgcaaatg cattagatat ggccgtcaaa cagccttcac aagcagtatt caatcaagaa
      421 ctctatcctg aaatctatga aaaagcggcg attttagcaa ttaatttagc taaaaaacat
      481 ccttttcata atggcaataa acgaactgct cttgttgcca tgttgttgtt tcttcaattg
      541 aataattgtc cagttgcttt ttctcgtcaa gaagctgttg attttatcat aatgattacc
      601 actagttcat tggatttcga ttcactaaag tccaagataa ccagttattt acgacaaacc
      661 gcaataaaat gaacgaatta aaaactttat ttctcaatta gatcagcatg tcattaagga
      721 atatgctgat ttttttatgt ttaatcatac aaaatagttt tttcataaaa atatggaaaa
      781 tatggattaa ttttgaaata attgatttaa aaggaataca aaagagtgaa tagtaggaaa
      841 tagtggttta gaactagtgg gctgattttt tatatatttt agtttacata atatatatta
      901 tacaaagtag atttaaaaaa tgataaacct ctctcgaacg gttttttatc aaaaatcgtc
      961 atttttccat caaaatctgc ttatgcaaaa ttataaaaaa attttgcagt ttttcttact
     1021 ttttttgtcg tttaattgaa tgataaaaac cagtttcttt attcagttaa tcaaatatga
     1081 acgaattaga tatgaatcat tgtatttgcc gttgcggaag gattttttaa ttaaccacag
     1141 ataaacattg ataacgattc gcttattgta atcaacagat taaatttcat atgttcatat
     1201 gtacggatac attttcggac cgattaattt taaccattct gtttatttgc ttacctatta
     1261 gatatcaata aaaaactgct taacagctat ttcagctgtt aagcagtttt aggattttta
     1321 gttcatattc attgtttctt ataatacagt gttcaatact tctcttaaag cacttgttga
     1381 tttgctgaac attgcttttt cttcttcgtt gattgataat ctaaacgaga ttgaccaggt
     1441 ttttggttga taccagctgt gataacaacg atatcagcat ctttacattc ttcatagcca
     1501 cctgaccata cagccacatt ttcatcgccc caagccatac catctaaaag gtccaatgct
     1561 tccccttctg ctttttcttg attcacatcg atcaatacta attcattaga gattccttgg
     1621 ttgatcattg cataagcgat acttgttcca acaaaacctg taccgacgat tactacttta
     1681 cgacttgttt ttttcatgaa taaacagctc cttttcataa ctatttgtta ctttcatgaa
     1741 aagtatagtc ttgtttattc attttatcaa catgttaatt ataatagttg ttattataat
     1801 ttttgatgga aacgaattcc tgaatacgca taacagataa atgacaaatg tttacaccta
     1861 tatcccctga taatacaacc aaaagtttag aactgaaaat aaatccaaaa aaattcgctg
     1921 caacgatata ataagacagc aatcgcacct aaaacaattt tccttcatat gctttagtga
     1981 aagattttct tcttaatgtt tatttctcca ttcattggga gtacatgttg aatacttctt
     2041 gaataatttg aataaagaag tcgtttgttt atatccgata cgattagcga tttcttccaa
     2101 cgaaaaactc gtttcttgaa tcagttccaa cgcaaagtcg aattttttct ttagaaccag
     2161 ttcttgaaag gaagcatcaa aatacatttt caatttatta ctaagataga ccggattgta
     2221 gccaaaatga gctgctgttt cttttaacgt acaagaagta aaatgcttat cgatataatc
     2281 taatactcta agcatctcat tttctgcgca tccatctgct gatgaaaagc tttgttgttc
     2341 taataaatga gtcaattcga tcagtaatag tgatgtatag agatttaacg aacggatctt
     2401 atgactgtat gtatcaaaat aaatcaacag catattttca atcaaatgcg tcaaacgact
     2461 tgtttttccg ccgcgaaaat gaatgaattg tgtatgttcc cctttttctt ttgatgcatt
     2521 tatcaaaaaa gaagaaagta gattatttgt gcttgccaaa tgattcaata tctctgtaga
     2581 taaggtttct tcttttaata agatgttgat taaaatatcc tcttgccctg tgtattcgat
     2641 tctttgagta gcttctttat caatgatcac cacgtcactt tcttccatct ctatcctttc
     2701 tccattaata atttgtaaac atgtaccgct atacatataa ttgatttcaa caaaatcatg
     2761 tgtatgctcg ggaacttccg catatcgacg gtgtttactg atatgaatat ttccctgttg
     2821 aaatagatcc cctcggtgga tttttggcgg tgtctgaaga tggtaagcta agtttaaatc
     2881 attgatattc attccttgtt cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa
     2941 aaatgtctaa ttcagctagt tccaattgac tccctccttt ttgtaagcgg tttctttcgc
     3001 ttattagttc acgctgtact gctattatat gcaaagctat ttttggtttc ataaagactt
     3061 tctatggttg ggacatgaac ttcctgtgat tccgctattg agagaataac cacttcatac
     3121 tatatttttg ataagcgctt acaaaaatga aaggaacatg cttatgaata aaaagatgat
     3181 gaaattttct ctgctgtcag tctctcttat tttgacttct gctggtgcag tcacgtctgc
     3241 aattcccagt atgtcagcta cttatgccac gcgttcttta tctgctgttg aattattaac
     3301 gactgctccc tcttttatgg tcactatttt tgttttgatt agtagtttta ttgctaggaa
     3361 aatcggacaa aaacaaactg tgatcatcgg attagtgatt gcatcgattt gtggtatcct
     3421 ccccctcttc tcacaaagtt acagtattgt cttagcttca agagctggac taggtattgg
     3481 ctttggattg atcaattctt tggcagtcag catgattgct gccttttatc acggagatga
     3541 acgagcctct atgattggtt ttcaaagtgc atttcaagga tttggtgcag ctttaatgac
     3601 atttgttgca ggacagctag tacagttcgg ttggcaatat acctttttag tctacgccat
     3661 cagcttacct attcttgtct tgtttgtttt gtttgttcct acacctgaaa caactgaact
     3721 tcaatcgcat ggcacagccc caacaagaca agtaatcaaa acgaatccta aaatgattag
     3781 ttatggtatt tttctcttgc tggtcatcgt ggtatataat gctgtatcga ttaaattagc
     3841 cacagtcctg acgacttatt ctctaggaaa cgaaactaat gcagcgaata tcttaacaat
     3901 catgcaattg gcaagtatgg ctgccggagt ttgttttggc tggcttcaga caaaagcaca
     3961 tcgctatatg cttacacttt ctttactact gatgggaatc ggctttgtat tattgccact
     4021 gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta
     4081 ttcattcctt acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc
     4141 aatacttcaa tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg
     4201 ctctttctct cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt
     4261 atcttgctgt tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag
     4321 acagtgataa gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg
     4381 aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac
     4441 tttgagatcc gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg
     4501 ccagcgtact ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt
     4561 cttgatcaac cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat
     4621 ggacaaaaac atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt
     4681 atctttgtag gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa
     4741 tcagatattg gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg
     4801 ctttatgtcg atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat
     4861 ccagtcactg aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat
     4921 ggctgtgtag aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga
     4981 gagtccgatg tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca
     5041 agaaaaatga aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact
     5101 ggaatggttc aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt
     5161 tcaagcgatt cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta
     5221 tgtagtgatt tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat
     5281 gcgtatcaat cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga
     5341 acgatattag cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac
     5401 tattcgatct tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa
     5461 tttttacgca tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg
     5521 aatgaattag ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa
     5581 atggataaag aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc
     5641 atgatcactt gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact
     5701 catctctatc aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat
     5761 gaagaacttg gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat
     5821 gcgaatattt tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta
     5881 aagaatgtct tattgaatga cactatcaca cagattacta ctccatattt caaattctat
     5941 gaacaagatg ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat
     6001 tattggggag ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact
     6061 caaacaggaa atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat
     6121 gcatggggag caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca
     6181 aagccaggtt atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat
     6241 tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg
     6301 attacttctg atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta
     6361 gcaaaacagc cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg
     6421 cttgcgacat atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt
     6481 tgaagtttgc ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa
     6541 agctagataa tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac
     6601 cgtattctcc tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg
     6661 tagaagaagc gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa
     6721 tcaataagct attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc
     6781 cgaagacacc agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg
     6841 tagtttgcaa gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa
     6901 agccaattaa tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt
     6961 tagaatcggg tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca
     7021 ccattcgaac cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca
     7081 gtgcaaagat tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag
     7141 ctaacaaagc aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat
     7201 ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc
     7261 caaaaaactt tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg
     7321 caacttgatt gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag
     7381 ttaatagtgt tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc
     7441 ccgcgcttac taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa
     7501 gaaaaatgaa tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta
     7561 gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat
     7621 tgctaggaac tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta
     7681 gtgaattact gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga
     7741 tagttccttg gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa
     7801 tcaaagaaac gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat
     7861 ccattgatag ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt
     7921 tcttttactt cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata
     7981 gaagttagcg tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat
     8041 acacttccaa catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca
     8101 agtgcttgtt tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact
     8161 gcaaaaattt tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt
     8221 tgatgcgtgt aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc
     8281 caaagcccga agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg
     8341 atgggctgga ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac
     8401 ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat
     8461 aaacgcacca tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt
     8521 attttctgat aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg
     8581 atccgataat tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc
     8641 cttcttttct aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc
     8701 tacgccttct tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg
     8761 cattgaaaat cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc
     8821 caagtcagtc atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag
     8881 aggtacagta gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc
     8941 tgaattagtc tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat
     9001 caatgaagag ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc
     9061 ttctgcgtcc gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc
     9121 ttcagggatg ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc
     9181 gtagatggaa gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga
     9241 ataggaaata cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg
     9301 aaacgaagca aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca
     9361 ttccagcaaa aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca
     9421 aagagagaaa aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac
     9481 ttgtcgacta atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc
     9541 catggatgtt tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat
     9601 tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac
     9661 tttggcgtaa taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc
     9721 aagtgactgc atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga
     9781 ccctagcagc ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa
     9841 ttcatttctt atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc
     9901 ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc
     9961 tcttcgctct tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc
    10021 ccagtcgatt gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa
    10081 aggagttaat tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc
    10141 tagactgatt tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt
    10201 cacctcctac ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac
    10261 ttacagttgg atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa
    10321 aaagcaggtg accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat
    10381 catgtttatt ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt
    10441 cgatatcttt ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg
    10501 aaccagataa agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt
    10561 cttctttgtc ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt
    10621 taggggccat tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg
    10681 ttcctcctag tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa
    10741 caaacccgat tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt
    10801 ccttcttcca aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg
    10861 ccattaacta atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa
    10921 attacttttt cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt
    10981 tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct
    11041 tcttgtcgat cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt
    11101 ggacgttctt tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa
    11161 aaatccagat cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag
    11221 ctgatatcat ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt
    11281 ccatataaat agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg
    11341 aaataatcat aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact
    11401 ttttggactg tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga
    11461 cctttttctt ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt
    11521 ttctctacta gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat
    11581 tgactaggtg tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc
    11641 tgatcagtat attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt
    11701 tttttgatat tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta
    11761 ctctttccat tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt
    11821 tttcagtata tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt
    11881 tcagaaaaac acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa
    11941 atcaaaaaga tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat
    12001 catgcttatt gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat
    12061 tgtcgattac atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc
    12121 aaaagctacg tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag
    12181 cggatttaaa gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt
    12241 caatcaaacg attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc
    12301 aaagaaaatg gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca
    12361 tgaagattta gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc
    12421 aaaaggtgat tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa
    12481 gtttttgatc atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag
    12541 cgaggattgc gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat
    12601 gacctatttg aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga
    12661 aatggccaaa caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa
    12721 agtagcgact ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt
    12781 gatatattct caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat
    12841 gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata
    12901 agaaagatag tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc
    12961 tttttcaaac ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg
    13021 aggaatagct aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg
    13081 aggaaaatca gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat
    13141 actttctgta cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca
    13201 agtaagcgat gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc
    13261 cctttttcga ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact
    13321 tgataagcga gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc
    13381 ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt
    13441 gtattttctc cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc
    13501 agcacagtat tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa
    13561 ataccgatcg tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta
    13621 gtaaagggat ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc
    13681 atgttttctt ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa
    13741 tgagagatga tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct
    13801 agtatcccta ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc
    13861 tttggtttca tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat
    13921 ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt
    13981 gcgtctagca aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac
    14041 atagattccc ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc
    14101 aggagaaatc aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg
    14161 gtttgttaat ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata
    14221 ttttagacct gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc
    14281 tttcgccgat acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt
    14341 gtcactatct tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac
    14401 gtcaccatct actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt
    14461 ttgatcattt gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc
    14521 tgctttttcg ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga
    14581 aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata
    14641 atttactggg tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga
    14701 atcattatca aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt
    14761 gttttgcgtt ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg
    14821 aattttttca ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt
    14881 agacgtatca tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa
    14941 aggcgtgatt gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa
    15001 attatatttt ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc
    15061 ggcaaagtca ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt
    15121 ttgatcagtt gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct
    15181 tggtgttcct tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc
    15241 agtaccgatt tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag
    15301 atggatagta tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat
    15361 ctcatataca gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc
    15421 ttctttgatc gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac
    15481 atttccatca gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac
    15541 tgcttgtttt gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt
    15601 gacgttataa actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat
    15661 ttctttcccg ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag
    15721 gatgacttga gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag
    15781 taatggtaaa ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat
    15841 tttttcactc ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata
    15901 ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta
    15961 ttttcttttt tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa
    16021 gttgaaatgg taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc
    16081 tcttttaatc gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg
    16141 gaaatcaggt gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc
    16201 tggaggtaat ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct
    16261 ggtttatttg tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt
    16321 gtactgacac caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt
    16381 gctgtactgc ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc
    16441 aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct
    16501 tctgtcagct tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct
    16561 tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc
    16621 gtatgcaagg aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat
    16681 accttctcaa aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata
    16741 gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc
    16801 ttttctttga cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt
    16861 tcttgatctt tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta
    16921 gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt
    16981 gcttcttcta ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa
    17041 acttggaatg tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg
    17101 tcatttggat gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc
    17161 tgaaccattt ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac
    17221 aagaagagac acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt
    17281 ttttcaaaaa aagataccaa atagaaaacg cacaaccaat catccctact agatagattc
    17341 ctaaacggcc aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta
    17401 aagaaatctg gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca
    17461 cttgaattga cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc
    17521 cttctggtgt aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa
    17581 aggtatctgt ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc
    17641 tgaattttgc tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa
    17701 acgcttttag tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt
    17761 ttcccgtgta aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag
    17821 tatcatatcc ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat
    17881 caaaactggc aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat
    17941 ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc
    18001 cttgcatcgc atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca
    18061 actggatcgt atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag
    18121 aagcgatctt gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg
    18181 tttctgtcag cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg
    18241 ttccgtcgcc atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag
    18301 cgccaataag atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg
    18361 tgaattgttt agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta
    18421 caggtaattc tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta
    18481 gtgataatat ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac
    18541 gttcccacgt atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc
    18601 aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat
    18661 cagctagatt gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag
    18721 ctttagcaga tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag
    18781 ttgtcctgcc gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga
    18841 ttctcacttg gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct
    18901 tgatggtatt tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac
    18961 tcttgactga aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt
    19021 gtccattgct tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat
    19081 ctgttgcatg atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt
    19141 gtcgcatacg agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt
    19201 ggatgccaag accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg
    19261 ttgcgataaa cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga
    19321 ttcttgagat tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt
    19381 aataattgct gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac
    19441 catccgtcag caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg
    19501 catctcttag tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt
    19561 ttacttgatt tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat
    19621 atccttcgct tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg
    19681 ctaaagtatc gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt
    19741 tcatacttcc tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta
    19801 catttccacg agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat
    19861 actttcgtat cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg
    19921 tccgatcatt aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt
    19981 tattgccttg atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat
    20041 aagtgccctg atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca
    20101 cagctggcaa ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg
    20161 atgattcttc ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt
    20221 ctgtctcatc aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt
    20281 ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga
    20341 tttccaacgt aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt
    20401 ccacatcact taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg
    20461 tgttattcgc atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt
    20521 cagctgaaat tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg
    20581 gcacaaagtt tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg
    20641 tggtcgtcat ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt
    20701 attaaaataa acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa
    20761 gggcgctaaa gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt
    20821 ccttcgatta atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca
    20881 ggttgttgga acgaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg
    20941 acaagtggat gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg
    21001 taaggtatat cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac
    21061 aaaaattggg gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg
    21121 aaatgtcgca aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta
    21181 aaacgattga gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt
    21241 tgaaacaatg caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt
    21301 ttcagttaac tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt
    21361 taagttacta cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa
    21421 ttatggtcaa gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat
    21481 ctttctttat tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg
    21541 aatggaaagc tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa
    21601 tcttgaaaaa tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt
    21661 ttgtcaatac gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga
    21721 tacgaaaaaa tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag
    21781 aatatgttgt tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata
    21841 cgactcattt gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac
    21901 gtctatatgc tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc
    21961 tgatttatta tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc
    22021 attatacttc tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat
    22081 atattgaaga atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg
    22141 ctattactga agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga
    22201 atcaacccat tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg
    22261 caaatgaaga agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta
    22321 taaaccaccc tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt
    22381 ccttcagcaa tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa
    22441 ttatttgcaa tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa
    22501 ctaactgctt cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat
    22561 ttttcgttca ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt
    22621 cgctgggtta acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac
    22681 cactctaaat atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt
    22741 aagatatctt gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc
    22801 aaatcttgaa aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct
    22861 gagactttga cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt
    22921 ctctgtttct ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct
    22981 ttatctctga ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt
    23041 ctcttcttct gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat
    23101 acggacatca tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag
    23161 taatttgacg acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa
    23221 gatcaagctt ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc
    23281 agaaaggtct ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga
    23341 atcgattttt gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc
    23401 ctttaccgtt aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa
    23461 gcgtaaatct gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat
    23521 ttctccggaa gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga
    23581 accagaagga ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt
    23641 caaagctgag acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa
    23701 tatttttttc atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta
    23761 cgtatcgaaa acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac
    23821 cattgtgacc aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa
    23881 gcaaacaaaa gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa
    23941 gatttagcga tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc
    24001 ttctgaagtg tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa
    24061 aagtatatca tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg
    24121 aatgtcccaa tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct
    24181 gaatccttta ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt
    24241 ccatacttca aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa
    24301 taagtttcag gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc
    24361 gtatctccaa ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt
    24421 gttttaaatt catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg
    24481 aaaactgtaa cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt
    24541 gctttctctc ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat
    24601 acttgatttg catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt
    24661 ttaaactctt ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc
    24721 aagaacataa taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata
    24781 gctagaaaca tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt
    24841 gaatagtgta actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa
    24901 ttgtctaaga catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct
    24961 tatttatgct ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa
    25021 actattagta gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc
    25081 aatcggcgag ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg
    25141 agtttatgat cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt
    25201 agcaaaaata cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag
    25261 tttggaagat aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt
    25321 ctatcgagaa gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt
    25381 tgatgaaaat gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt
    25441 gtataaagaa caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt
    25501 acaaaataag ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga
    25561 ttctgatacg acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg
    25621 aagtactgga ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag
    25681 ggtgtgatgc aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa
    25741 atgtagcagc atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa
    25801 accaatctgc atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa
    25861 cagtacaatt agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa
    25921 cggaacaaaa cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta
    25981 acactcaagt gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg
    26041 ggcgtttcaa ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa
    26101 atattgaatt aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa
    26161 aagcggagac gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa
    26221 tcaagaattc attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga
    26281 ttctctctat tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt
    26341 cttggagaag acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa
    26401 cgattattca aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag
    26461 aatccgaaag catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg
    26521 atttgctgac aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca
    26581 atacacaaga atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg
    26641 gaaaagagtt tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa
    26701 tccatcaagt tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata
    26761 agatcatttt ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg
    26821 gcccttctat cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc
    26881 aatctcgggc gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa
    26941 tcgaacagca tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta
    27001 aagtccaatt gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca
    27061 aaaatcttgt cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc
    27121 ggaaataagc cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc
    27181 ttacgatttt ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc
    27241 ttctcggcta acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc
    27301 aacttcttca cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat
    27361 agtaatcgtg tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg
    27421 tttgcgttcg atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt
    27481 gaccacatgt ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa
    27541 tttttcttct tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc
    27601 agtcttgctt gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg
    27661 tgtcatattc aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg
    27721 atctgttcca gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc
    27781 ttgtgtatcc attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac
    27841 aaattttcca tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc
    27901 cgtctcagaa tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg
    27961 gtcttcttca ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc
    28021 aaatcctagt ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata
    28081 cccttcgtta cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc
    28141 gatgaccgtt aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg
    28201 gtatattgtt acttataaat ttattc
//
LOCUS       DACESX010000025        27490 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.025, whole genome shotgun sequence.
ACCESSION   DACESX010000025 DACESX010000000
VERSION     DACESX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.025"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="IXF88_000921"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="IXF88_000921"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1507664.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="IXF88_000922"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="IXF88_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1507665.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="IXF88_000923"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="IXF88_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1507666.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="hflB"
                     /locus_tag="IXF88_000924"
     CDS             complement(4246..6357)
                     /gene="hflB"
                     /locus_tag="IXF88_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1507667.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="IXF88_000925"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="IXF88_000925"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1507668.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="IXF88_000926"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="IXF88_000926"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1507669.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="IXF88_000927"
     CDS             complement(8557..9027)
                     /locus_tag="IXF88_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1507670.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="IXF88_000928"
     CDS             complement(9073..9498)
                     /locus_tag="IXF88_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1507671.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="IXF88_000929"
     CDS             complement(9565..9834)
                     /locus_tag="IXF88_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1507672.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="IXF88_000930"
     CDS             complement(9845..11440)
                     /locus_tag="IXF88_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1507673.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="IXF88_000931"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="IXF88_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1507674.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVVSEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="IXF88_000932"
     CDS             complement(14993..15553)
                     /locus_tag="IXF88_000932"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1507675.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="IXF88_000933"
     CDS             15906..16880
                     /locus_tag="IXF88_000933"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1507676.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="IXF88_000934"
     CDS             17050..18450
                     /locus_tag="IXF88_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1507677.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="IXF88_000935"
     CDS             complement(18528..19250)
                     /locus_tag="IXF88_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1507678.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="IXF88_000936"
     CDS             complement(19329..19835)
                     /locus_tag="IXF88_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1507679.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="IXF88_000937"
     CDS             19958..21757
                     /locus_tag="IXF88_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1507680.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21825..23399)
                     /locus_tag="IXF88_000938"
     CDS             complement(21825..23399)
                     /locus_tag="IXF88_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507681.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23654..24163)
                     /gene="mreD"
                     /locus_tag="IXF88_000939"
     CDS             complement(23654..24163)
                     /gene="mreD"
                     /locus_tag="IXF88_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1507682.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24167..25021)
                     /gene="mreC"
                     /locus_tag="IXF88_000940"
     CDS             complement(24167..25021)
                     /gene="mreC"
                     /locus_tag="IXF88_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1507683.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVASVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25176..25814)
                     /locus_tag="IXF88_000941"
     CDS             complement(25176..25814)
                     /locus_tag="IXF88_000941"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1507684.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25815..26465)
                     /locus_tag="IXF88_000942"
     CDS             complement(25815..26465)
                     /locus_tag="IXF88_000942"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1507685.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26478..27377)
                     /gene="rsgA"
                     /locus_tag="IXF88_000943"
     CDS             complement(26478..27377)
                     /gene="rsgA"
                     /locus_tag="IXF88_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1507686.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
BASE COUNT         7556 a         5932 c         4994 g         9008 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttctcctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg ggatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag agacgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta tacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa ataaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggtcga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgattaatc attcttacat gctgacagca
    21841 aagtcagggg caaaccattg aactgaacca acttttacgc tttcgcctgg ttgaggagca
    21901 tggatatatt gtcctccgcc taaagcaatc gctacgtggt aagttccgcc ttgtgacccc
    21961 cagaataaca agtctccagc ttttgcttga gaaactgaaa tttttgttcc agctgattct
    22021 tgaggaactg tccaaccacc gatgtcacga ccagttactt gcatgtaaac atagcgtgtg
    22081 aaccctgagc agtcaaagcc acttggatct ttaccgcccc aaacatatgg agtaccaata
    22141 tatttgtaag cttctgctac gattgctgca ccattcacac ttccagaagg agctggagca
    22201 ggtgttacag gttttggttg ttcaggtgtt ggtgttggcg ttggagccgg agtactactt
    22261 cctgtgccgt ttcctgtatc aacactttga tctgttgatg gtgttgtagg cgcaggtgtt
    22321 gttgattctt ctgttgttga agattcaggc actgttgttg attcttctgt tgctgaagat
    22381 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcgctgttgt tgattcttct
    22441 gttgctgaag attcaggcgc tgttgattct tctgttgaag attcaggtgt tgttgattct
    22501 tctgttgctg aagattgtgt agctgatgat gattcttcag tcattgaaga ttgtgtagct
    22561 gatgattctt ctgttgctga gctttgtgca gtagatgaag cttgagtagc ttgtgcttca
    22621 gcctctgctt gttcgcgtgc ttctttttca gctttttctt gtgcagcttg ttgacgagct
    22681 tgttctgcta gacgttgttg ttcacgaata cgagcttgct ctgcttcagc ttctgctttt
    22741 tgacggttca agtcagcttt cttgtcttca gcagttgctt gttcagcagc taatgaagtt
    22801 ttcagtacgt tcaaatcagc ttgtttagat aaaagatcgc ctttttgaga ttccaaagca
    22861 gcttggtttt cagctaattc ttttagttta gcatcgtttt cagctttttt gtcttctaca
    22921 gcttttttat cttgtttttg ttgttccatc aagtcgttgt ttgcttttac catcgttgtc
    22981 attgcttgaa cgcgtccaat agcatctgct aatgaatcag cattcaatac agcatcgatg
    23041 tagtttgagc ttgtgttgct tacttgtgct tcgcgtgctt gtttttggat tgtatcttca
    23101 cgtttttcaa tacgttcttg taaatctgca atgtctttta ctaattgtgc agattcttga
    23161 cgcaatgtat cttgttttgc taataggtct tgtgcttgtg tgttgatttc agaaacttgg
    23221 ctttctaagg catcgatttg tgattgtgca tctgcttgtt gatttttcaa gtctgcaatc
    23281 ttttgatctt gctgttgtat ctgagaatca aaatcgtccg ccgcggcagc gatcggtgat
    23341 gctacggcag tcaaggtcat tgaacatacc attactgctg atattaaact ctttttcact
    23401 cttcattcct ccgactggct taatttaata aatatctaaa tatatttaag tctttcttaa
    23461 cgatataaaa attgtaccat agcagtgtgt caacgatata atagttatgt tacaaaaatg
    23521 tttcaaaaca acttttaaaa atgacaaaac tgttcgtttc agaaaatgat catgaaaaag
    23581 ccgcgttaag tctagcattt cttaagataa caacgattct ttttcgcttt cgacctaaaa
    23641 ataaaaaaag aaattattca tcactgaata atttcttaaa gggaaaaatg aataatacaa
    23701 atataatcat gttcaacagc agtgtaggtc ctaaaaacct tgtaataaaa tacgtgttat
    23761 ttactactgc taatttaaaa atcaactgga ccaccatcgt aaacaattca taacccgtga
    23821 caaagatgat catactgaaa aattcagtaa agatgttgac atggataacg tctttcatta
    23881 catacatcaa ccatacaatt aaagggagag ctactgcata gataccgata acgccaatat
    23941 agtaagcgtc atagatagcc ccgagaacaa tcgtggtaat cagtaaataa cgtttttcaa
    24001 acgccatact gcaacataat aatgccaata tcagaaagtg ggcatttgac atataggtgc
    24061 ctttcgacca ctcccctagc attcttgtta aatgaccatc tatcaacatc aaaaagaaca
    24121 aaacgattgg cagatagtac ttcattgttt ctttcttcaa catggactaa tcctccgcta
    24181 gccgttggac aatcgtcaca actgacacgt catacatttc tgcataaggt ttcacataaa
    24241 cttcccgatc cagcccataa ctatctggtt tcgttttaat aaccgtaccg atcggcaagt
    24301 tagctggaga attccctcca agaccggatg tctggacaac atccccttct ttgatatccg
    24361 tatcaccagt taattgggtc accactaagg catggagctt ttcatcatag tttttaagca
    24421 aaccaaacgc ttcgccatta gccgaagata cccgtactgg aaaatgattg gagctttcat
    24481 tagaggatga cagtaattcg attttagacg aagccgtatt gacctcgatc actcggccaa
    24541 tcaatccttt ttgcgacatg actgccatat ttgcttcgat accgtcttta gaacccttgt
    24601 ctacgatcag catgtcctgc caactgtcag gcgaacgagt aatgacattt gccgttactt
    24661 tttcgtaact agtcagcgtc tgattcaact caagttcttt tttcaacgct tcgatttctc
    24721 tctcgtaatt cttgttttgc tgtgaaacgt catcatagct atcgattttt tcttttagcc
    24781 gctcattttc ggcataagtt gaaaaaagat tatggacaga agctactcca tttcgtaccc
    24841 accgtactgg ggcagagatt gttttatcaa ccattgcaac actgtcatta acgatggatt
    24901 ggaagaaatt gggctttcct tcattcgctc gctgcgcagc ggtcacactt agtatcgtca
    24961 ccacgatgat aacaataacc aaggtaataa taatattttt attaggattg aacttcttca
    25021 caaagacacc tcgaattaat tagactcttg tgttcaattt tatcactaaa tgccgtgaaa
    25081 caaaaggtaa gttgctcctc ttaagctttt ttttagcaaa taatagattc tgtcattttt
    25141 agcctgaaag acaaattttt ttatttgttt atggatcaat ctttgctttg aataactgca
    25201 atcataccat ctgaaaaaga aaattctgcg gtatctgaaa gaaattcgtt acttgcataa
    25261 gatgtcggat attctacttc tttattttcc aatacatact tgctgccttt taatgtaaga
    25321 ttcgagacgg gagtcaagca gcaataagct aaatacttca tatctggtat ctgtcttact
    25381 atatgctctc caggcaaata ataagacaaa taattttgac aatctctgat aataagctga
    25441 tgggcaaagg gtttgaagcg aggttccata cctaaccaga gattggctaa aagatgatcc
    25501 aatcttccgc ccgtagctcc aatcaaagtg acttccgctt gcgggtaacg ttcaaatgtt
    25561 tttaagatac ctaactgtgt atctgtatcg tctttttctg caggggaacg ggtatactct
    25621 ttggcttttg caaatacttc cttctgctct tcgttcgaca atgaatcaaa atcacccact
    25681 gccagatcta ctgggtagcc tgcttgaagc aggtacagcg caccacggtc tatcccgaca
    25741 taggcatcgt atttggacag cagagactgc ggccagtttt gcggattacc tccagcagct
    25801 aataaaattt tcatttagtc taatgcctct tttaatcgtt cgatttgtcc gactggattt
    25861 tcggctccat agatatatga tcctgcaacg aagacatccg cccctgcatc tttacagatt
    25921 tttgctgttt ctggaacgat tccgccatct acttcgattg cataagtcca attgttctct
    25981 ctacgtagat cagcgagttc cgtaattttt tctactgttt cttcaataaa gctttgtccg
    26041 ccgaacccag gattgacagt catgactagt acttgatcag ccaaaggcaa aacatgtttg
    26101 atcatggaga ctggtgttcc tggattgata actactccag ctttaactcc taagcttttt
    26161 gtcatttgaa gtgcccgatg aatatgagga gttgcttcga cgtggacagt aatgatatct
    26221 gctccagctt ttgcaaatgc tggaatgtag ttttctggat caacgatcat cagatggacg
    26281 tctaacggta atttagtaac aggacggatt gcctgaacaa tatttggccc taatgtgata
    26341 tttgggacaa attggccatc cattacatct acgtggatgt aatctgcgcc tcctttttcc
    26401 actaactcaa tgtctctttg taaattggca aaatctgcac tcaaaatgga tggtgctatc
    26461 ttcattcttg tttcctccta ttttcgttta ccttcttttt ttgcatacat tggttttctt
    26521 ttttcgattt cttgtaagaa ttgcaaataa ttgtcgtaac gtgtctgtgc gataccacct
    26581 gtttctactc tatgttttac ttcgcagcca ggttctttag catgcatgca ctctctaaat
    26641 ttgcaatgga cagaagcttc tacaaattca ggaaattgtc tcggcagttc tcctgcttca
    26701 attgtcaaaa agtcaatcga gctaaatcct ggtgtatctg ctactaaccc gccatagata
    26761 ggaatcagtt ccacatgacg tgttgtatgc cgtcctcttc caagtgcatc agaaatctct
    26821 cctgttgcta aattcagttc cggtgatatt tgattcagta gcgtcgattt tcctgctcca
    26881 gactgaccca taaagaccga tagccgttct gggaagtaac ggatcagttc tgtcgtatca
    26941 cccgtatgat tgggtacaat caccggatag cctattttag tataggtttg cttgatctct
    27001 tcgatttttt ctgggtcgtc tgacaagtcg gtctttgtca aaaaaataat cggttcgatc
    27061 gactcgtatt ccaaggtaac tagaaagcgg tcaagtagat tataagaaaa tgcaggctct
    27121 acgatacttg tgacaatgat tccaagatca acatttgtga ctggtgggcg aactaattga
    27181 ttttttctcg gcaagatttc cagaagatag ccgtctgttt gattttcact ctcaaagatc
    27241 acctgatctc cgacaagcgg cgtgattttc cgattccgga aatttcctct tcctcttgtt
    27301 tgataggtct ctccgtttgc gtatacatag taaaaaccgc tgattgcttt tctgatttgt
    27361 cctttgataa gtgccacctc cgtttttgtt tattttacca tacgaagagg aaagtaggtg
    27421 ttattccatg caaacttctc aaagtatatg gcttgaaaat tgatcaaaag agatagaaac
    27481 acaaaaaaga
//
LOCUS       DACESX010000026        27314 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.026, whole genome shotgun sequence.
ACCESSION   DACESX010000026 DACESX010000000
VERSION     DACESX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.026"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(38..634)
                     /locus_tag="IXF88_000944"
     CDS             complement(38..634)
                     /locus_tag="IXF88_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1507687.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            984..1328
                     /locus_tag="IXF88_000945"
     CDS             984..1328
                     /locus_tag="IXF88_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1507688.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1329..1748
                     /locus_tag="IXF88_000946"
     CDS             1329..1748
                     /locus_tag="IXF88_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ1507689.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1840..2190)
                     /locus_tag="IXF88_000947"
     CDS             complement(1840..2190)
                     /locus_tag="IXF88_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507690.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2156..3487)
                     /locus_tag="IXF88_000948"
     CDS             complement(2156..3487)
                     /locus_tag="IXF88_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1507691.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3742..4308
                     /locus_tag="IXF88_000949"
     CDS             3742..4308
                     /locus_tag="IXF88_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1507692.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4453..4540
                     /locus_tag="IXF88_000950"
     tRNA            4453..4540
                     /locus_tag="IXF88_000950"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4485..4487,aa:Ser,seq:cga)
     gene            4876..5850
                     /locus_tag="IXF88_000951"
     CDS             4876..5850
                     /locus_tag="IXF88_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1507693.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6175..8070
                     /gene="asnB"
                     /locus_tag="IXF88_000952"
     CDS             6175..8070
                     /gene="asnB"
                     /locus_tag="IXF88_000952"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1507694.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8265..8711)
                     /locus_tag="IXF88_000953"
     CDS             complement(8265..8711)
                     /locus_tag="IXF88_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1507695.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8939..9088
                     /gene="rpmG"
                     /locus_tag="IXF88_000954"
     CDS             8939..9088
                     /gene="rpmG"
                     /locus_tag="IXF88_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1507696.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9176..9694
                     /locus_tag="IXF88_000955"
     CDS             9176..9694
                     /locus_tag="IXF88_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1507697.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9771..10727)
                     /locus_tag="IXF88_000956"
     CDS             complement(9771..10727)
                     /locus_tag="IXF88_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1507698.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10893..11699
                     /locus_tag="IXF88_000957"
     CDS             10893..11699
                     /locus_tag="IXF88_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507699.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11696..12685
                     /locus_tag="IXF88_000958"
     CDS             11696..12685
                     /locus_tag="IXF88_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1507700.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12682..13686
                     /locus_tag="IXF88_000959"
     CDS             12682..13686
                     /locus_tag="IXF88_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1507701.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13818..14360
                     /locus_tag="IXF88_000960"
     CDS             13818..14360
                     /locus_tag="IXF88_000960"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1507702.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14380..15078
                     /locus_tag="IXF88_000961"
     CDS             14380..15078
                     /locus_tag="IXF88_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1507703.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15099..15311
                     /locus_tag="IXF88_000962"
     CDS             15099..15311
                     /locus_tag="IXF88_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1507704.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15311..16273
                     /locus_tag="IXF88_000963"
     CDS             15311..16273
                     /locus_tag="IXF88_000963"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1507705.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16291..16686
                     /locus_tag="IXF88_000964"
     CDS             16291..16686
                     /locus_tag="IXF88_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1507706.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16849..17214
                     /locus_tag="IXF88_000965"
     CDS             16849..17214
                     /locus_tag="IXF88_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1507707.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17211..19022
                     /locus_tag="IXF88_000966"
     CDS             17211..19022
                     /locus_tag="IXF88_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1507708.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19084..19251)
                     /locus_tag="IXF88_000967"
     CDS             complement(19084..19251)
                     /locus_tag="IXF88_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1507709.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            19500..20489
                     /gene="galE"
                     /locus_tag="IXF88_000968"
     CDS             19500..20489
                     /gene="galE"
                     /locus_tag="IXF88_000968"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1507710.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20501..21985
                     /gene="galT"
                     /locus_tag="IXF88_000969"
     CDS             20501..21985
                     /gene="galT"
                     /locus_tag="IXF88_000969"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1507711.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            22009..22989
                     /locus_tag="IXF88_000970"
     CDS             22009..22989
                     /locus_tag="IXF88_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1507712.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            23078..23902
                     /gene="tsaB"
                     /locus_tag="IXF88_000971"
     CDS             23078..23902
                     /gene="tsaB"
                     /locus_tag="IXF88_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1507713.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23886..24428
                     /gene="rimI"
                     /locus_tag="IXF88_000972"
     CDS             23886..24428
                     /gene="rimI"
                     /locus_tag="IXF88_000972"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1507714.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            24435..24899
                     /gene="rimI"
                     /locus_tag="IXF88_000973"
     CDS             24435..24899
                     /gene="rimI"
                     /locus_tag="IXF88_000973"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1507715.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24896..25912
                     /gene="tsaD"
                     /locus_tag="IXF88_000974"
     CDS             24896..25912
                     /gene="tsaD"
                     /locus_tag="IXF88_000974"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1507716.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(26484..27185)
                     /locus_tag="IXF88_000975"
     CDS             complement(26484..27185)
                     /locus_tag="IXF88_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1507717.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         8877 a         4693 c         5642 g         8102 t
ORIGIN      
        1 acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga ccacttagat
       61 attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt ttagcaatca
      121 aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc aaacataacg
      181 catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca aaacggtttt
      241 gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg gaaagtatga
      301 atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg cagattccaa
      361 tatagttata gataaatcca gcataagggc caaatatcag gacaccagct gcagtactga
      421 tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca aaaataatcg
      481 gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct ttgaaaacac
      541 ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata atggatatga
      601 aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt agaggttctt
      661 tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata tgacagaaaa
      721 caaacaattt tgtaagttca tcataagtat aaaagattga tctcaaaaaa acatccaact
      781 aaagacttat ttaataactt gtttttttta aaataaaaaa agcgttagca actatgctta
      841 aaaagcatat cgactgaata aactaagcat tggacaggct gaaagactac atttaaaatg
      901 aaatgcgaag aaacaataga aaataaagtt gccttagaac catagttgaa tcacctaaca
      961 gtcgtgaagg gagaataaga gaaatggcag aaaaacaaac cgcaggacga gatcaattag
     1021 gagattttgc accaaaattt gcagaattaa atgatgatgt tttatttgga caagtatggt
     1081 ccagagaaga attatctgct catcagcgta gtatcatcac catatctgca ttaatttcag
     1141 gaggaaattt tgaacaattg ccagctcatc tgaagattgg caagcaaaat ggcattacta
     1201 gtgaagaaat agcagaaatc attacccatt tagcttttta tgtagaatgg ccaaaagctt
     1261 ggtcagcttt tagtttagca aaagaaatct atttagaaga aaatagcgaa gataagaaag
     1321 aggcgtaaat gatgacaaaa cacgaagaaa tacacaatgg cgtgattttt ccagcaggtg
     1381 aaaaaaacga agcttacgct caagtattta ccgggcaaag ctatctgaat atgttagtga
     1441 atgatcccga agtttctgtt ggtgtaggta atgtaacttt tgaaccaggt tgtcgcaatc
     1501 actggcatgt ccatacagac ggctatcaaa tcttacttgt gaccggagga gaaggctggt
     1561 atcaggaaga aggaaagcca gcaagacgat taatggctgg agatgtcgtc gtaacgcaca
     1621 aagggattaa acattggcat ggtgcgacaa aagacagttg gtttgaacat ttggcgatca
     1681 ctgctggtaa ttcagagttt ttagaacctg taacagatga agtgtatgaa ggattggaaa
     1741 atcaataatg tcaagcttga acagctcctg aaagtagatt ttaatcgaac tttcaggagc
     1801 tgcttttttg attcagtctt ttataaatag aaaatagggt taataatagg aatgagaagc
     1861 acgtcgaaag atcttaatat tgataatcgg ctgggcatac atgatttcca cgtcatcaat
     1921 caaactacct aacagatgat aatcttcccg atcatgtcgg ggattaccta atagttcttg
     1981 atagtagatt tccatactag ggtcccgagg attgttcagc aaatcagcaa gtacatctaa
     2041 atcttctggc tgagtttctg ccgtaccctg tatctctatg aaaagccctt cctcagcaaa
     2101 ttctactcca atacgtattt cagttatttc atgggaatat aaataagtaa gaagctcatc
     2161 gatgattctt tttgataatt ctttttccaa gtgtcttttt ttcctttctg acagcggtaa
     2221 agttatctag tatcggtaca aggatacctg caacgaaacc agttgaaaaa ccactgttat
     2281 atagatttag tcctccgtgc aaagtgctga cgttatggac aagagcaata tgtagcacac
     2341 cagctattac accgtaaaaa ctgccgtaaa aaccgcttat gggcgcaaga cctgtcccaa
     2401 acacagctgc aacaagcatg ccagtactgg tgatctcatg aaaagttcca aacaaagaag
     2461 caataaatat tccgactagt atcggaaaac tgttttttaa atgacaacca aaagcactga
     2521 aacccaccgc agataaaata gcaccgatga caggtccatt taactgaccg ccaaccagca
     2581 aaacataaga agacagcatc agaccaacta agcccatgtt gatcaaagta gcaccaatac
     2641 cagaattagc aatgaaatca gttgtcaatt ttccagatga atcgaaaatt tctcgaatcc
     2701 ccgaaagccg aaaggaatta taatagaaac caattccgac tgtcagcgaa aaaaagccaa
     2761 aaataatcca aatgagagga aagtgatatt cattgaaaac caatgtattt tcgacgattg
     2821 aatagctgaa taacctcaaa acagcagtaa aagtcatcgc aatcaaccca gaggtgaatc
     2881 caatattata aagtgtgaat ccacgatgga aaactaaagt ttgagcagct aagggcggaa
     2941 gaagtatacc aattgctatg ccagctaaat tccccaataa aatacctatc agtaaaggaa
     3001 aacgcatacc aaaagtgata taactaacga ccggtgataa tacgctacca aacagtccga
     3061 tcatgatgta ctgagaaaaa ggtttattga caaatacagc gtatagatac acacctaaaa
     3121 tgattgagat agaattataa aggtttttgc caaaaaaaga gaaaccggta actgttagaa
     3181 tcgaagcaaa taagggaccg tttaaaatca ccttgtgccg ataagctaaa agcacagaaa
     3241 gtaaggtcaa aatccctaca tttatgaatg cgcttccgaa aacacctaaa gcaaaataat
     3301 ctgtcaaaag attgcttggc gaggtaagaa tagttagcaa tccactccaa ttatcagtag
     3361 gagtattaga aagcaagcca ataaaaagaa aaaaaacacc tgtaaaacca agataaaaat
     3421 actttctttt ctgtgaaacg attcgttttt gaggatgaat ataaataacg tcagtttcgt
     3481 tttccattac acaccttctt tttcattttc tttaactata acttgaaatg aaagatttgg
     3541 acaactatca tttaggtcaa aactaaaaat gtaagggaac ccattgaatt taaccaaatt
     3601 tttttaattt tgcaaaaaaa agttaaagaa gactgaaaaa aatttaaaat ataacgtttt
     3661 gagtaaaatg tttgcaaaaa atgctaaaaa tagggtacaa taaaacaaca tatactgcag
     3721 taaagaagtg ggggagtttt aatgagaaga aaagtctata ccaaggatca aattctaaaa
     3781 gctgcttatg atgttgttgc aaaggaaggg tttaaaggat ttactgcgag aaatattgct
     3841 aaaaagatgg ggatttcgac acagccgatt tatctggaat tcaaaaacat ggaagattta
     3901 aaaaatacgt tgctggattc tatctttgaa caactaaaag aagatgtttt ccctattgaa
     3961 cgtactggtg ataaaatcat tgatctcggt ttgaactata tctactttgc aaaaaagcat
     4021 catagtcttt acatggcatt gtacattgat gaacatgggg acggaaagaa aatgcatgat
     4081 ttttcataca attatttccg tgaaatagtt aaagaagatg aaagatacaa tcaattatcg
     4141 gatgaatatg tcgatgctct gcatactggt acatgggtag cagtgactgg cttagcgtca
     4201 ttgatgtcat caaacatcat gcatccaaca gaagaacaaa tcattgcact gatcaaacgg
     4261 acaattgact cgattttagc attagataat cctgaaataa tcagatagtg ttctatcagt
     4321 atgaatttga tcggtaagtc cgttggctgc aaactgtcaa tggacttttt tgctaaaaaa
     4381 agcgatttct gttatttatc tattagaaat aggcttgaca tttcgtgaaa aaagtttaaa
     4441 aatagtactt acggaaggtt ggcagagtgg taatgcaccg gactcgaaat ccggcgaacc
     4501 ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct tatatcaacg attagaggtt
     4561 gtgaagaagc tgtcctgcat caagtaataa gaacggccaa acaaaaatag cttttcctat
     4621 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttgttcgga
     4681 ataaggggct gtgacaagac ttttgtcaca gccccttatt gttgtacttt taacctacaa
     4741 tattttttga ttccccctga gtactatttt ttataaatgc tagtaaatca atcttctttg
     4801 gccttttttt tgataaaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt
     4861 ttaggaggga aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga
     4921 aggaattttg attatgagat gtcttataat gaagtagtca ctattactcc aagaaattat
     4981 cgtttcgatt ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca
     5041 gctggggtat ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg
     5101 gcaggactga attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac
     5161 gttgcaccat ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct
     5221 aaagaattat tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg
     5281 tcacctttac actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa
     5341 gatggacttc atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat
     5401 tatcaattat ttaatctgaa caattatcgc tcgctatcaa atggaacacc agaaaatcat
     5461 ttttcaaacc agatcagctt gaatgtttat agtcgtggga tgggaggatt gggcttacct
     5521 ggagatctct cttctgtttc tcgatttgta aaagccacat ttactaaaat gaatgctgca
     5581 tcaggtgatt cagaatctga aagtatcagt caattttttc atatactagg ctcagtcgag
     5641 caacaaaaag gagtatgcga tactggagaa ggaaaatacg aatatacgat ttattcttct
     5701 tgctgtaacg ttgataaagg gatttattat tatcgtacgt atgaagacag tcaaattact
     5761 gctgtaaata taaataaaga agacttggat cgtcatgaat tggtcagtta tccagtcata
     5821 aaagagcaac agataaacta tgtcaattaa ttaatgaata gatccgtttg gtatcatatt
     5881 ttctgataca gtcataatta gacacgatct ttaggtcgtg tctttttttg ctgttttttt
     5941 tggaaatagc atccactaat atttttttaa cacgaagaga ataaggtagc ctcaaaaaag
     6001 aaaacatggt aaattagata ttgggacttt attttctgta aaaacagaaa ctttcatctt
     6061 ttgttttcac gtgacgaaat tgtaagagag aaaatattcg tccgctattt attctatgat
     6121 aagatgcata tgaatgaaat aaacagtaat tttatattag gatgtgaaag ttaaatgtgc
     6181 ggtattgtag gttttgttaa cgacaaggac aacaaaaaaa caattattaa tacaatgatg
     6241 gatcgaatcg ttcaccgtgg acctaatagt tcaggagaat atatcgataa acatgttgct
     6301 ttaggattca gaagattaag tattattgac ttagagggcg gtactcagcc tatttacaat
     6361 gaagatcgga caaagatcat tatttttaat ggcgaaatct ataattacca gcctttgaga
     6421 gaagaattga ttgcagctgg ccacgttttt caaacacatg cagatacaga agtccttttg
     6481 catggttacg aagaatgggg aacagaatta ttgcaaaaga tccgcggaat gtttgctttt
     6541 gctatttggg ataatgaaaa gaatgagtta ttcggtgcaa gagaccactt tgggatcaaa
     6601 ccatattatt atgcagaaat gaacggaaca tttatgtttg gttcagaaat caaaagtttt
     6661 ctgcctcatc ctgattttaa caaagaattg aacaaagaag cgttgaaacc ttatatgact
     6721 ttccaatatt ctccattaaa cggcgaaaca ttcttcaaag atgtctatcg tttgcctgaa
     6781 ggccattatt atacgtataa agatggaaaa ctagacattc agcaatattg ggatgcagat
     6841 tttgaaacaa aagaaacgca ttcccgacaa gaatggatcg aaaaaatcga cgaaaccgtt
     6901 caagcttcta ttgaagcaca tacggtcagt gatgtagaag ttggttcttt cttatccagc
     6961 ggggttgatt ccagttatgt aacctctgta ttgaaaccag atcattcttt ctcaatcggg
     7021 tttgatgata aaacctataa tgaagcgatt gaagccagaa aactaaccga actattggac
     7081 ttagataata cagcagcagt gatcgacgga gacatgtcgt tcaaagcatt ccctttgatc
     7141 cagtatcact tagatgaacc tgattcaaat ccttcttgcg tacctttgta ctttttagca
     7201 aatcttgctt cacaaagtgt gcgggtagtc caatctggag aaggggcgga tgaactgttt
     7261 gcgggatacc aaacgtatgg attccatacg aattcaaaat tcatccgtgt gattgctcaa
     7321 ggattgaaaa aattaccaaa aggaacacgc tataatttag gacgtaaaat cggaaaaatg
     7381 aaaaatttcc atggacggat ccatttatat gaatcacttg ctcctgcaaa agaatatttt
     7441 atcggtcatg ctcgagtgtt cgaagaatca gaagcatcag aagtgttaac accaaaatat
     7501 caaacagcac catctgtgga tgaaatcatg actgttcatt acgagaaaac agaaggaatc
     7561 aaagatgaag tcaataagat gcaatatgtc gatcttcatc aatggatgcc aaaagatatc
     7621 ttgttaaagg cagataaact ttctatggca agttctttgg aagtccgagt acctttattg
     7681 gatatcgaag tcatgaaatt ggctcaacaa ataccaagta agtttttatt gaatcaaaac
     7741 aacacaaaag atattttcag acaagctgcg aacaaacatc ttccggaaga atggtcgaat
     7801 cgtgtgaaat taggattccc tgtgccaatc aaagcttggt tgaaagaaga acacggctac
     7861 gaacaagtca aagcattatt cgaagcagat tttgcaaaag aattttttga tcaagaaaaa
     7921 atcatgaagt tgctagatga ccatcacgaa ggacgtaaag aagagcaaag aaaaatctgg
     7981 acaatcttca gtttcttgac atggtataaa gtctactttg tagatgaaac gattccgcaa
     8041 gcagaagcaa tcgactatgt gactgtttga tgaaaatcaa atatcaataa aaaagaggtt
     8101 ttctcctacc gtttaacact gagtgataag aaacaatttc aaaaaacagc tcttcccgtt
     8161 ttatagatat tatgacttat ggttcggagt tggcagagga acaccattgt ttcgaacata
     8221 agggggttgt gacacgattt tcgtcacaac ccctttacat atgttcaata atcgatttct
     8281 ttttcttttg tcccatcttt tttataaatg aacaaagttg attctttgtt ttgtgctact
     8341 tgctttcctt tttcgattgc ttcttttttt gtatcaaaat ggttgcttgc cttttttgcc
     8401 cctttagatt tcacgatcca ttgttcttcc tcatattcta cgatcacatc tgaatcaagc
     8461 agttttccag cacgaggatt cgtatcgtgt tcatccgtct tgcttggatt tttttctttt
     8521 tcgaaagttc gtttttctga ttcactagca tcttcatacc atttttttgc ttgatcaatg
     8581 gcaattggga tcgcacggtt atcagggtac ccttcatcta ataatgcatt tgcgatgtcg
     8641 attgcttttt ttcttgtcaa tttatctaag tttttcattg atgcaggaaa gtccttcatg
     8701 ttccatggca tattagtatc cccctttatt aaaaaacata atctatataa acaagtgtaa
     8761 gggtatttat acaaaaagaa aaagattttg tttgagaagt aaaaccgttg tatttatcgt
     8821 caagtagttt tacttaacaa aatcgtgaaa aacactagca atctagaaaa aatcatggta
     8881 agataatcaa gtctgagagt catcttagag gattcttgta aaaatgggag ggaaaaacat
     8941 gcgcgtaaac atcactttag agtgtacttc ttgtaaagaa cgtaactatc taacaagcaa
     9001 aaacaaacgt aacaatcctg atcgtttgga aaaacaaaaa tattgcccac gcgaacgcaa
     9061 agttactttg caccgtgaaa caaaataatg tttttaaagt tctcaattcc ttgctggtca
     9121 aagaattgag acttttttta tctcaaaaca tctaaaatca taaggagcgg ataatatgtt
     9181 tatttccatg tgggcacaag ataaaaatgg tctgatcggc aaaaatggtt tgttaccatg
     9241 gcgattgcca aatgatatgc gtttctttcg cgaacataca atggatcgaa ttttagtgat
     9301 gggaagaaag acctatgaag gaatggggga tctttcactt ccttatcgcc atatcatcgt
     9361 gttgacaacg caaccaaatt ttcgcacgaa agaaaatgcg gaagtaatgc attcgatcga
     9421 tgaacttttg tcgtatagtc aaacgatctc agaagatatc tatgtttcgg gtggtagtcg
     9481 gatttttcaa gaacttcttc cttacactgg tcggatctgg cgaacattga tcgatagcac
     9541 gtttgaaggc gatacgtata ttgggaatat tgatttttca gacttcgccc ttatagagga
     9601 gtatgagggg ataacggatc aagaaaatct atatgcccat cgttttcaaa aatgggaaag
     9661 agtaaaaaat agggttgctt caaaagaaga ataaaatcta tgcgtctagt ttgttaataa
     9721 gaaaaaaagc atctcacgac taatcgtgaa gattcttttt ttagttaaaa tcaatttagt
     9781 ttttctttga tatcattgac catttcctga ctggcaatag gtccgttata tagccaacta
     9841 gattcaggtc cgaattgcag tacatgattg tctgtcactg ctttaagatt cttccagctt
     9901 ggttcattga acatggcagc atctttgtca ctgttcacaa ggatgatgta gtcagcatct
     9961 aattcagcta gcttttcaga ggaaacagca gaccagtcag aagtggcttt tttggagacc
    10021 tcttccacta gatcaggaac accaaattcc agatcgtcgt atacgattct tccacttgac
    10081 cgatttttgc tgaccataaa ggctgagttg ttggtcaccc acaacactgc agctgtcttg
    10141 ttctcgattt tatctgctag ttcttctcga gttttcttga tggatttttg ataatcggaa
    10201 atgatttttt ctgcctcttt ttcttttgcc aaagctttgc cgacatcttt tagttgttct
    10261 tcccatgtga catctgttcc gttttttaca acataagttg gcgcgatttt gctatattgt
    10321 tcgtatttcc cgccttcgac agtagcggaa gaagaaatca acagcaaatc aggctcaaag
    10381 cttaacactt tctcataagg aaggtcataa gaaatcgttg ggacatcctt taaatcatct
    10441 tgcagataat cttgaatgga accactgcca acagtccact gggcgatagg tttttcccct
    10501 agtgcaatca gatagtcttc taagtaactg ccgatgatcc gtttgggcgc gtttggaatc
    10561 tctactttat gtccaagtgt atctgtgact gtatgagtac tattggatga agtcgtcgaa
    10621 ttggcagttg tagctgtttc tttatctgaa gagggggagc aagcgctcag tactccagcc
    10681 agcagcaata tgctagataa tgtgaatact tttgttttta atttcatttc tttcctccta
    10741 acataattga gaatctttct cattggttat tattcctgat atttcgaacg ctgtcaaccg
    10801 cttcatttgg ctatagatat gaaagtagcg cttgacgaaa tgttgaaaca gcgtcataat
    10861 tgagaatgat tttcagcgaa aggatgggtg aaatgacgag attaagagca gctgacattg
    10921 cagccggcta cgaaaacaaa aaaatcattg aaaacatgtc tgttacgatt cctgaaggga
    10981 aaatcactag tttgattggg ccaaatggaa gtggaaaatc tactttattg aaaagtttta
    11041 cccgtatact acctgtgaaa tcaggaaaga tcattgtcaa taactctgat atctctacat
    11101 acagttcaaa agaacttgca aaaaaaatta cattattagc acaaactagt gaacacccta
    11161 caggtatgac agtagaagaa gtagtatctt acggacgata cccttatcaa aaactgttca
    11221 gtggcatgga cacgcaagat catgaagcaa tcgagtgggc gttggatgct acaggtctag
    11281 ctggatttcg tatgcgggat atcgcaacgc tgtctggtgg acaaacccag cgtgtctgga
    11341 tcgctatggc gcttgctcag gaaacagata tcttgatctt ggatgaaccg acaacatttt
    11401 tagatccggc acaccaatta gagatcctac agttattgac tgaaatcaat caaatgaaac
    11461 aaacaacgat cttgatgtcg attcatgaca ttaatcatgc ttcacgtttt tcagattatt
    11521 tattagcaat gaaaaacggt aagttgatca cagaaggtac acctgaagaa gtgatcacaa
    11581 aagagtgtat gaaaaaaata tttgagattg atgcaacgat tgtcacgctt ccaagaacaa
    11641 ataaaccagt ttttctaacc tatgatttgt tgccaaaaga gcaggaggat caattatgag
    11701 gactatgaag aaaaatagac tcctgctttt ggtctgtttt gttcttttga ttattttttt
    11761 tgttgctctt tcaaatggcg cagcgactgt gggaaaagca gatttttggc aggcgttgtt
    11821 tgattttgat agatcaaaac aaagtcacca aatcatacga aatattcgtt tgcctcgtgt
    11881 ggcgggagca tttcttgtag ggagttgttt tgcattgagc ggtgcattga tgcaaggagt
    11941 gacaagaaat cctttagcag attcaggctt actaggaatc aacgcgggtg cttcgttggc
    12001 aatggccctt agttttgctt tttggcctaa agcttctgat ttttctgtgt ttctaatgtc
    12061 attggcaggt tcttttgctg taactttgct cgtatttggt tttttgcagt tttcgaaaat
    12121 gggcatgaat cctttacagc tgattttagc tggcgtagct attagttctt tttttactgc
    12181 tatcagccaa gcacttaccc aattctttaa tctgcagcag gatcttgctt tttggtttgt
    12241 aggcggagct gccaatatca catggaaaca attagcagta ttgtttcctt tatatatact
    12301 tgctgttttg gtttcttttt cattaggaaa agcaatatct ttgattcatt tgagtgaaac
    12361 acatgcatta gctcttggaa gtcatcctaa acgaacgcgg ttgattgttt tctgtctagt
    12421 tgcagtgtta gcagccattg ccgtttcgtt agtcggacct gtttcattta ttgggctgtt
    12481 ggttccgcca gttgttcgcg gaatggttgg aacggactat cgttatgttc tacctgtctc
    12541 ttttttagtt ggcggtggat tggttatgtt ggcggatttt acagcgagga tgatcaatcc
    12601 cccttttgaa acaccttttg gattagtcat tgcattgatt ggcgttccgt ttttactgat
    12661 acaagtaagg agggaacaac ggtgaaaaaa atatattgga cgttctattt cttgcttttt
    12721 gtacttgtca tcctgtctat aggtagttta atgattggta cgccttttat ttacctggat
    12781 cagctttttg ccacgttcat agggaaagaa tcatcttctt tggcgctgat tgttagagaa
    12841 tttcgagttc ctcgattact gatcagttta ttagcaggag cttgtcttgg cgtttctggt
    12901 tatatcttgc aaggagtaac aagaaatgaa ttggcagatt ctagtatatt gggcattaat
    12961 gccggagcag gatttttagt gatgctttat ttaggtttct tttcgcacta ttcgctgccg
    13021 tttctattgc tagttgtagc ctttctaggt ggtgttttag ctgcttgctg tgtctatctt
    13081 gcggcctatg atcgaaatgg ctttttgggt atgaatcgaa tcttattgtc cggtattgcc
    13141 gttaatgccg gcctttctgc attaacattg ctatgcacga tccaattatc taaggaaaat
    13201 tatggatttg tgaatgcatg gttagcggga tcaatctggg gtgcgagctg gccttatgtt
    13261 tgggcattgc ttccatgggc ggttatctta attccgctag gaggacttta tgcacagagg
    13321 attggattgc tatcatttgg tcaagaacgc gcgcaaggat taggccttaa cgtggctaaa
    13381 agtcaaaaaa tccttttgtt gttagctgtt gctttggcaa gcggtagtgt agcggtgaca
    13441 ggtagtttat cttttgtagg actactagct cctcatgctg cgaagctagt cgtacgtaaa
    13501 gaaaataact ggacctttgt tctgagcggt ttgttcggtt ccatttttct gcttagtgca
    13561 gatatggttg ctagagtgat tctaccaagt ggggaagtac ctacaggaat tttgattgcc
    13621 ctttttggtg cgccatattt tctgtatgca ctttttatca gacaaaaaca cgcgctgaac
    13681 agttgaaatt tattaaaaaa ggaaggccaa tcatttctag gtcttctttt tattctattt
    13741 atgcatttat ttgaaagttc gattgctatt tttctttttt tcctatacca ttttatgaga
    13801 gaaggaaggg tgagtttttg gaccaattga aagtaagatt acgccggaaa ggaatcgaaa
    13861 agctgaaaaa tctggccaaa agtggcaaga aaaaggaaaa agaacagcga atctataaac
    13921 agctattcgc cagcgagtac tggaagtcag caaaagtgat agggacgact ttatcgaatg
    13981 aatttgaact ggatacacag ccggttatcc aacgagcaga aaaagaacaa aagaaaatag
    14041 ttgtacctaa aactttgccg aaacgtcaga tggcgtttta tgaaatggat gaacagtccg
    14101 ttcttgtacg ttcgagtttt ggtgttcttg agccacaatc agagaagctc tatttgccgg
    14161 agatgatcga tctcctcatt gtaccaggta ttatctacaa tagagccggt tatcgaatcg
    14221 gttttggcgg tggttattac gaccggtatt taaataattt tcataaaaaa acatgtagcc
    14281 ttgtttttcg tgaacaattg aatgaacaat ggcaaccgga gagctttgat cagaagatcc
    14341 aacaattgtt tattgactaa aatgaaatgg gaggttttca tgaattatca acagcaaata
    14401 aaattgcgtg catggttaag acgaccgtta tggacctatg cttttttagg gatacagaca
    14461 gcggtcttcc tcatgatgga attatttccg cgacttgaga tcccttatta tgccggaatg
    14521 tatggtcctt atcttgttca cttcaatgaa tggtggcgtt tagtgacacc aatcttcatc
    14581 cattttggac tgatgcattt cgtaatgaac tcactgatac tgtattttat ggggcagcaa
    14641 atcgaggcaa tctatggcca ttggcgtttt ttcttgattt atatgttcag cgggataatg
    14701 ggaaatacag caagctttgc ttttaacgaa gcgaatgttc tgtctggtgg tgcgagtact
    14761 tcgatattcg gtttgttcgg tgcgttgttt atcttagggt ttcattttaa acataaccca
    14821 gcgatccagc agctagtcag acattttctt ttatttattg tgttgacatt tgtttttggt
    14881 cttctggata cctctgtaga tgtttgggga catgtcggtg ggctaattgg cggattagtg
    14941 ttagggaatg ttttgggtct accgaaacaa cagacaagct attcgataca ccaaaggatt
    15001 ctttctacac tagtttttgt atttcttttt gtaatatgta ttttactggg tttaaaaaaa
    15061 tatggtttac ttgtataata tgaaggaagt gcttaaagat ggagagctta tatgatgtac
    15121 agcagctgtt caaaaaattt ggcatcttta tctatgtggg tgcacgcatc tatgacattg
    15181 aattaatgac tatcgaattg cgtaatctgt atgatagccg tttgattgat catgatacat
    15241 acatgacagc gtggcgcata ttaaaaagag agcaccgaat tgaagaaagc cgaaagaaag
    15301 gaaatttgta atggacaaga agattattgg gattgattta ggtggaacaa cagcaaaatt
    15361 tgcaatttta acaccagaag gagagattca acaaaaatgg agcatcgata ccaatatttt
    15421 ggatgatggt aagcatatcg ttcctgaaat cattgaatca atcaaccatc gtttgaattt
    15481 atataatatg aaagccgaag actttatcgg aatcggcatg ggtaccccag gaagcgtgga
    15541 cagcgaagct ggaacggtta ttggtgcgta taacttaaac tggacagaag ttcaatttgt
    15601 gaaaaaactg atcgaggcag gaacaggaat caaattcgct atcgataatg atgcgaatgt
    15661 tgctgcttta ggtgaacgat ggaaaggtgc tggagaaaac gatccagacg ttgtattcgt
    15721 tactctagga acaggtgttg gcggtggtat cgttgctggc ggaaatttga ttcacggcgt
    15781 gagcggagca ggcggagaga tcggccatat cactgtggat cctgaagggt tcgaatgtac
    15841 ttgcggtaaa cgcggatgtt tagaaactgt gtctagcgct actggtgtgg tacgtctagg
    15901 tcgttacttg gcagaagaat acgcaggaga ttcaaaactg aaagcaatgt tagacaacgg
    15961 tgaagaagta acaagtaaag atatctttga aatggctcaa gaagacgatc cgtttgcatt
    16021 gatggttgtt gatcgcgtat gcttctactt aggtttagct tgcgccaact tggggaatac
    16081 attgaatccg tcaagtattg tgttaggtgg aggagtatcg gcagcaggag aatttttacg
    16141 tagccgtgta gagaaatatt ttaaagaatt tactttcccg caagtaagag aatcaacaaa
    16201 aatcaaatta gcagagcttg gtaatgaagc tggtgtcatt ggtgctgcct cattagcctt
    16261 gaaatttgct taaaaaaacg gaggcattac atggttttat gggtaattaa tatcattttg
    16321 ctattgattg tgcttgcaat catcttttgg gaagtctatc tacgagtaat ggccaagcgt
    16381 tcagcaacga ctttgacaga agaagaattt cgagaaggaa tgagaaaagc acaagtaatc
    16441 gatgttcgcg aaaaagatgt gtttgatgct gggcatattt taggtgcaag aaacatccct
    16501 tacactgttt tgaaagattc attaggatct attcgaaaag accagcctgt ctatatttac
    16561 gatcaaaaga aaagcttgag catccgtaca gcaaataaat tacgtaaagc aggttatcaa
    16621 gagatttatt tacttaaggg tggatatgac ggatggtctg gaaaaatcaa aagtaaaaaa
    16681 gtataaaaca gatgattgta tgacagcttt aaaaaccgaa tcaatcatta gattttgacc
    16741 aagatcacat gaacggcgca atggatagtt gtgccgttca ttttttttat tttgtgctat
    16801 tatctacata tataagctaa gcgaaaaggc agaaagaagg aaacaactat gatcaaatta
    16861 aaacgtgttt accttgatgc tgaaaaagag gatggctata gaatactcgt ggataggttc
    16921 tggccacgag gaatttcaaa agaaaaagcg cagttagatc tgtggttgaa agaaattgca
    16981 cctagtaatg aagcacgcaa aacttttgac catgtaccag aacgatttgc ttcatttgca
    17041 aagaaatacc agcaagaatt gattttcgaa gaagaaaaga agcaagctat ttttaaactc
    17101 agtcaactta tgaaagaaca tccagtcatt acttttgttt atggtgcaaa aaatgaacat
    17161 cagaaccatg ctgttttatt gaaaaaattt atcgaaatgg aggcatgcaa atgaaaatag
    17221 ggatcattgg tgccggacct agaggattaa gtatagcaga aaggattctc cataataatc
    17281 aggacagaca ttctcttgaa ctttttcttt tcgatcccaa tggtcctgga ggtcgagttt
    17341 ggcggttgaa tcagccaccg gagttattaa tgaattctgt ttctcaacaa gtgacgcttt
    17401 ttacagatga aacattgtcc agtggaggat tggtaacaca gggacccaac ttgtttcaat
    17461 ggagtaaagg acctgctaca gattatatcg aacagacggt aaccaaaaat aagcaactaa
    17521 ttatagaaga aatcaatcgt ctggaaaaag atcagcaatc tactcgatgt ttatacggac
    17581 tctatcaacg ctggtttttc tcaaagttgc aagaagaatt tccagcttct attcagtttg
    17641 tttattcttt agtaaaaaaa gtcagcaaaa gaaaagatgg ttatctttta cagacagatc
    17701 agcaaagcta ttctatcgat cagcttgttt tagcaagcgg tcactgggaa aatgatgaga
    17761 cagaagaaga aagagtgtgg gtagactacg caaagaaaaa tgatttactg tatcagcttc
    17821 cttctaatcc tgcagatgtc gatctttcga agataccgcc taaagaaaat gtctttttaa
    17881 gaggtctagg cttaagtttt tttgattatg tcggattgtt tacagtatca agagaagggc
    17941 gcttcgagaa cgaaaaagga aaacttgtct atcatccatc aggcaaggaa ccgattgtat
    18001 acagtggatc aagaaggggg ctcccttatt accctcgagg aagaaaccaa aagcaaggcg
    18061 gatcaatggc atggccacgg ttactaacaa aagaaaacct ggagcaatgg catcagtcgg
    18121 gatttttgac tggcgaaaca ttttttgact atctgaaaaa agacgttgag ttgttttatt
    18181 acaagaaaat catcgaagaa tatcatttgc cgattccaaa gaaaaatttt gagcatgatt
    18241 ttctctccct ctcttcagag gagtgtctcg gaaagcatcc agcactaatc ccttatagat
    18301 gggattggtc gtatttagaa attcctctta cctatcagga tgagtccttt gcagaagcta
    18361 gccgagcatt tattgaaggg cagataaaag aagcggaaaa aggcaattgc acaggagcat
    18421 ttgcttcaac atttgatgca ttaaaagact ggcgtgatcc aatccggcag gctattgaat
    18481 ggcacgtttt ttctgcaaag gaatacaaag aaatactatg gggatggttt actcatctaa
    18541 atagcttttt aacgatcggt ccgccagtca ttcggacgaa agaattagca gcactgatag
    18601 atgcgggtat ttttcatttg ttcgaaccgc catttgatat tcagcaaaag cacaaccgct
    18661 atgttatttc tctacaagat aaagaaatca gcagtcgcta tttgattgaa gctcgattgc
    18721 cacaaacaag cttttctcgt tcaagtaatc ctgtcataaa aaatttgaag gaaaacaaac
    18781 ttgttcgccc tttttcattt tcagataata caggaagtta tcattcagaa gctatagatg
    18841 ttgagcgagg tacgaatcgc gtgattgatg cttcagggga aatccagtct gacttttatt
    18901 gctatggaat acctgttgaa ggtgtggatt ggctaactgc agcagtagct agaccttata
    18961 cagatccatg gaatctgaga caggcagata aaatagcaca acttatattg actgacaaat
    19021 aaaaatacgc atgatgactt atctgttctt tcacaagtct tcatgcgtgt ttttaaataa
    19081 ttattaacga gaaacgcgtt tgcgcattgc ttttttacgg ttttcttcga tcattgcttc
    19141 tttttcgtca attggatcga tcactgtttt tttcacagaa gcgaccaaac cagcaacggc
    19201 agcggctgtt gccagacttc ctactaatat tccagataca aattttttca ttgaatccac
    19261 ctcatttatt atttgttata tctattatgg aacaaaagag aaaaaaaggc tagttttacg
    19321 tttgtttgta aacctttgca aaaaatcctt gaaaatgttt actaaatgtt ttattataaa
    19381 tttaccatat gaatttacga aattttactt tgtttgtttt caccataaaa gtaaacttcg
    19441 tttttttaaa ttatatataa tcaacaaaca caattttact aatcaaagga gtgtttttta
    19501 tgacaatttt agtactaggc ggagccggtt atatcggttc gcacgctgta gaccagctag
    19561 ttcaaaaggg gtatcaagta gctgtggtag ataatttgct cacaggtcac aaacaagcag
    19621 tacatccaga tgctcatttt tatgaaggag acatccggga taaagaattt ctacgttccg
    19681 tatttgaaaa ggaaccgatt gaaggagtaa tccattttgc tgcaagctct ttagtaggag
    19741 aatctgtcga aaaaccattg atgtatttca acaacaacgt atacggtatg caaatcttac
    19801 ttgaagtcat gcacgagttc aatgtgaata aaatcgtgtt ctcttctact gcagcaactt
    19861 acggagaacc aaaagagtct ccaatcacag aagatacacc agctaatcct aaaaatccgt
    19921 atggtgaaag taaattgatg atggaaaaaa tgatgaagtg gtgtgaccaa gcatatggca
    19981 tgcgctatgt cgctttgcgc tacttcaatg tagccggagc aaaagcagat gcatctatcg
    20041 gagaagatca tacaccagaa acgcatcttg tcccaatcat tttacaagta gctcttggtc
    20101 aaagaaaagc attggctgtt tatggagacg attatgatac accagatgga acatgtatca
    20161 gagattatgt tcaagtagaa gatttgattg ctgcacatat tttagctttg gaatacttga
    20221 aagaaggaaa tgaaagcaac ttcttcaatt taggcagcag caaaggatac tcagtcaagg
    20281 aaatgctaga agcagctaga gaagtgacag gaaaagaaat tccagcagaa atagcaccaa
    20341 gaagagctgg agatcctagc cgattagtcg cttcaagtga aaaagcgaga gaggttctgg
    20401 gttggaaacc tgaatacaca gatatcaaag caattatcaa aacagcatgg gattggcatg
    20461 tcagccatcc aaatggttat gaagaatagg ggaatgagat atgtcaatca gtcaaacgat
    20521 ttgtgatttt gccacacttg cgatcgaagc tggcggatgg atggaaatgg accgcttata
    20581 tttacagaat cgtcttttag ggatggtagg agaagatgct ctagaaagca ttgaagtaag
    20641 accagttaag gaagaatctg tagacttatt ggatcagctt gtcgctaagg caagagaaaa
    20701 tggtgttctt tcagaattgc cagcagaaca agagattttt gaagcgcaat taatggattt
    20761 tctcacaccg ccaccttcag tcgtcaatgc attctttgca cagcactatt cgaaaagccc
    20821 agaggaagca actgagtatt tctttaattt aagtaagaaa aatgactaca tcaaaacaag
    20881 agccatcgca aaaaacatca aatttccggc agagtcggag tatggtgaac tggaaatcac
    20941 aatcaattta tctaagccag aaaaagatcc aaaacaaatc gcagcagaac gcgaggcggt
    21001 aaaagtagac tatcctacct gcatgctttg catggaaaac gaaggatata gaggtcgctt
    21061 gaactatcca gcccgcacca atcaccggat tattcgaatg aatctagacg gtgaaagctg
    21121 gggattccaa tattctcctt atgcttatta caatgaacat tcgatcattt tatcagaaca
    21181 acaccgcccg atgaaaatca caaaggaaac atttgctaga ctgcttcgga ttactgaagt
    21241 cttgcctcat tactttgttg gttcgaatgc tgacttgccg attgttggag gatcgattct
    21301 ttctcatgat cattatcagg caggacgtca tgaatttcct atggcaaaag caccgataga
    21361 aaaacttatc aaactagcag attatccgca agtcatcgca ggagtcgtga aatggccgat
    21421 gtctgtgatc cgtttgcaat caacagagaa aaaattgttg attgaagcag ctaactcagt
    21481 attggaaaag tggaaagcat attcggatga aacagtatct atcagagctt tttcagcaga
    21541 tggtacacca caccatacga ttacgccaat tgcacgaaga agggacaatt actttgagtt
    21601 ggaccttgtc cttcgagaca acaatgtttc agaagaacat cctgatggga tttttcatcc
    21661 acataaagat gtccaacata tcaaaaagga gaacattggg ttgatcgaag taatggggct
    21721 tgctgtttta ccacctcgtt tgaaaccaga actagtagaa gtagagcatt tcttattagg
    21781 agaagaaaac cagatggctg aatatcatcg tccgtgggca gaacaattaa aaaaagaaca
    21841 tcctgatttg acgaaagata cggtcaccga tgtcgtacaa aaatctgtag gtcaggtttt
    21901 tgcacgtgta ctggaagacg ctggagtatt caagcgagat gaaaaaggac aggcagcgtt
    21961 tgaacgattt actgcaattt tataaacgta aaaaaggagg agagacaaat ggcaacaatt
    22021 aaagacattg cgcagttggc aggagtttct cctgctactg tttcaagagt tctcaactat
    22081 gatcctgatc tttccgttgg cttagccaca aagcaaaaaa tttttgaagc agcagaacgc
    22141 ttaaattata cgaaacatcg taaaaatgca gcaaatacta agcaaacgct tcgcctgatc
    22201 cagtggtacg acgatacgga agagcttgaa gatctttatt atttatctat tcgtctagga
    22261 atcgaaaaaa aggcagaaga agcaaacgtt actttattga aagaaacgtg ggattccatt
    22321 agtgatcttc cgacagatgg aacgatcgca ctaggaaaat tcgacgaagc tcagttatta
    22381 gaaatgaaag gatcccaaaa tgccttgctt ttggtagatt ctgacggcac ttctcaagga
    22441 atcgactctc tagttgtcga ctttaagtca agtgttcaaa aagtcattga acattttgtc
    22501 tcccataaag cgcaacatat cgctatgctt gctggaacag aatatacgaa gaaaaaccat
    22561 cagcggctaa aagatcccag aattatggca tttcaacaag ttttggcaga aaaaggctac
    22621 gacgaaggtc caattatcga agcagatttt tctgtcgagt caggatatca agcagtgaag
    22681 cagtatttaa aaacgaatcc gcatgttcct gatgcgcttt ttgcagctaa tgatgcatta
    22741 gcgattggcg cactgaaagc gattcaagaa aaaggtttag tggtaccaga agatatttct
    22801 gtgatgggtt tcaatgatgt cagtgtagcg aaatatgtaa cgccctctct ttctactgta
    22861 aaagtttata ccgaatggat gggagagcta gcagtggaga cgattcttca attaagtaat
    22921 tccaaagcac cggtttcaag aaagattatt gtggaaacag agttgattat tcgagaatca
    22981 acgaaataaa agatggaaaa gctgtgggga aatgaagtga taccgcagct tttttgtgtt
    23041 tcaggaaggg gcaaaatttg cccgccagca gataatcatg tatcattgta ctagttttgc
    23101 ctttggtatt ttcatcctca gaataaaaaa gtatgctaca atggaaggcg agaatgtcat
    23161 agcagaaagg atcaaatata tgattacatt aggaatagat actgcaaacc aaactttggc
    23221 gatcggtgtg gtagaagacg aacaaattct tggacaaata caaacaaata tcaaacgcaa
    23281 ccacagtgta acactgatgc ctgcaatcga ccaattgttt gcagatcttc aaataagtcc
    23341 aaaagacatt gaccgaatcg cggtttctga tggtcccggt tcttatacag ggcttcgtat
    23401 aggtgtaacg acagcaaaaa cgattgctta tacattagat aaagagctag taggagtatc
    23461 tagtctgaaa accatcgcgg ctaattgcat cggtgtggat aaaattattg tgcccatgtt
    23521 tgatgctcga agaaaaaatg tatacgctgg ggcataccgc tggaaagaaa atagattaga
    23581 aattgtactt gaagatgtac atatttctgc ttctgatttg ttcacacaac tacaaaagct
    23641 gaatgaagct atttatttcg tcgggagtga ttgtcacaaa ttcttctatg aaataaagga
    23701 aatattgcct agtgcgatga ttaatacagt tccagcatgg gatattccaa gtggtgtaac
    23761 tgtggctcaa ttaggtgctt tagctgaacc agttgcagag aaacaagctt ttcttcctcg
    23821 ctatttaaaa cgggtagaag cagaagaaaa atggttggag acgcacacac ctggagacga
    23881 aagttatgtt gaaaaaattt agagcgtatg ccagaagctt ttttcaagat aaaatgattt
    23941 atacagaaaa aacaatcgaa caaatcgatc gtcgttacgt actaagagaa ttgactacag
    24001 aagatatcaa agagctgctg gatgtcgaac gagaagtcta taatggcgaa ttgccatgga
    24061 caaaaagtgc ctttatatca gaattgaagt caccgcttcc tcatctatat ctaggtatct
    24121 ttgataatgg aagaatgatt ggttttatcg gttcaagagc gttcggttta gattgccata
    24181 tcacgaatat tgccgttcga ccggcatatc agcgtaaacg aatcggttcg ctgcttattg
    24241 acgaaatcga gaactttgct ataatgaatc gctgtgagac gatgtcatta gaagtgcgta
    24301 tgagtaatca agatgcacaa agactttatc ggaagttagg ttttcaagca acaacgatca
    24361 aaaaaagata ttacactgaa acaaaagaag atgcgttgga tatgataaaa cttttagaaa
    24421 atgagtgaga ttagttgatt tgcagaaaaa aagattttga tgaagcgtac ttggcagaat
    24481 gcgtatggat gattagcgaa aatgcttatc cacatggttc accttggacg aaaatgcagt
    24541 ttttggcaga catccaacag cctcactctg attatctgct tttagttgaa gataaccaga
    24601 tctgtggctt tattggatac tcgaaagtaa tagatgaagt cgagatcaca aatattgctg
    24661 ttgcagtttc agaacaagga aaagggcatg cacggcattt gttacaatta ctgattacgg
    24721 aacaaataaa agattcgctg catgtttttt tagaagtacg tctatctaat gaagcagcaa
    24781 gaaaacttta tgaatcagaa aagtttcgta tattaggtaa acggaaatca tattatcgca
    24841 atccgacgga agatgcgatc attatgagca gaaagttaaa aacggagacg atgaaatgaa
    24901 tgaagaattg attctggcga ttgaatcaag ttgtgatgaa acgagtgtag cggtcgttag
    24961 aaatggaaca gagatcttat ctaacatcgt tgcttctcaa ataaacagtc ataaacgttt
    25021 tggtggcgta gttcctgaag tagctagccg ccatcacgta gaacaaatca ccttatgctt
    25081 ggaagatgca ctggtagaag ctggtgtgtc ggcagaagat ttatcggcag tcgctgtcac
    25141 ttatggacca ggacttgtcg gctcgttgct gattggtatt tctgcagcta aggcttttgc
    25201 ttgggcacac caattgccgc ttattcctgt aaaccatatg gcagggcata tctatgcagc
    25261 ccgtctggtt aaaccattcc agtttccatt gatggcactt cttgttagcg gaggacatac
    25321 agaattagtc tatatgcaag aagatggatc atatgaaatc attggggaga caagagatga
    25381 cgcagcagga gaagcttatg acaaagttgg aagagtatta ggtctttctt acccaagcgg
    25441 aaaagaaatc gatcagctag ctcatcaggg aaaagataat taccattttc cacgagcgat
    25501 gatccatgaa gataattatg atttcagttt tagcggatta aaaagtgcat ttatcaattt
    25561 ggtgcataat gcacaacagc gtggagaaga tctagacaaa aatgatttag cagctagctt
    25621 tcaagcaagt gtgatcgatg tattgattac taaaacactt cgagcttgtc aaaattatcc
    25681 agtcaagcaa ctgattgtag ctggaggtgt agcggccaat caagggttgc gtgaaggatt
    25741 gcaggatgct ttggcaacta aactccctga ggtagaacta gtgattcccc ctcttcgact
    25801 ttgtggagat aatgctgcaa tgattggagc agcagcacat gtggaaatgc aaaaaagaaa
    25861 ttttgctagc tatcagttga atgctgatcc aagcttggtt ttatctgaat aaagaggttg
    25921 tgaaaaatct gccctgcatc aagtaataag aacagcccag gaaaaacggc ttctcatgtt
    25981 tttgacctgg ctgttcttat taccgcgtat gcaagctttt gaacgccgtt taatcaagag
    26041 ttcgtaagcc tgtcgtttaa ctctgattca caaggaacaa tttcaaaaaa cagctcctca
    26101 tgtttatgaa cattatgact tgtgaccgaa gagttgacag gtacacaccg tttaatcgaa
    26161 ataagggctg tgacacgctt ttgtcaaagc cctatttttt acagcttatt catcattatc
    26221 cgttcctctg attgccctca ctgattaatc gtttattgtc gaggaatatc gaataatcta
    26281 ggtcggatga ggacaagacg cttcctctag ttaatctatc agtatttttt gcaaacagtg
    26341 aatattacat aactgaaaca cacatagaaa atctttgagt cttgcccagt gatctaaaac
    26401 tgccggatct ttcagaagtt ttagggagga aaataagcag tgaccctgat aaatatcagg
    26461 gtcactgctt atttttgttc gcgttattct ctcatagctg gagtagtagc tgtcttacag
    26521 aaagtaaaac gctttttgtc ataatctaac agtccggaat cacgtagttg tttgattact
    26581 tgtccagcag tttcacgtgt cgtgccgcta ttcactgcta attcttttaa ggtgatcgga
    26641 taaggaatgg tgatatgacc atcttttgtt ggtacgccca attcttcttt caaaatgaat
    26701 aaattttgtt ttacccggtc aaaggcgcta gaggtcaatc cgatttggat acgcaattca
    26761 tgtatttcaa ctactttaga aattttttgt atcatataga tcaactgttc gggatttttt
    26821 cgtgctaatt tttcatagta ggaaacagga aggtagaatg tttcaatatc agtcatcgct
    26881 tgagctgtat aagtgtaggt ctcttctcca aacattcctg acataggaaa aaggttattt
    26941 gtattcacat agtcatagta gtaataagtt gcactttcat cataacgttc taaacgaacg
    27001 agtcctttgg tcaagatata caaccgttgt ctgggatcac catcgtcgaa aaggatctgt
    27061 ccttttttat aagaacgaag aacagtatgt tccttgatct gttggaactc ttcatccgag
    27121 aaatgttcaa aatcaggttg atatctgagt tttgaaaagt gaaaatcgat tttattaaca
    27181 cgcattaaaa agtcctctcc ttattgttca ctatatttat tgtcactttt tttaaagaat
    27241 ttgtaaagtt ttaacatacc aacattagaa acaagtttta atactttaca aatataattt
    27301 gttattatgt aaaa
//
LOCUS       DACESX010000027        24578 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.027, whole genome shotgun sequence.
ACCESSION   DACESX010000027 DACESX010000000
VERSION     DACESX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.027"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(152..550)
                     /locus_tag="IXF88_000976"
     CDS             complement(152..550)
                     /locus_tag="IXF88_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1507718.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="IXF88_000977"
     CDS             complement(547..1320)
                     /locus_tag="IXF88_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1507719.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="IXF88_000978"
     CDS             complement(1317..2000)
                     /locus_tag="IXF88_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1507720.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="IXF88_000979"
     CDS             complement(2167..3054)
                     /locus_tag="IXF88_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1507721.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="IXF88_000980"
     CDS             complement(3051..3920)
                     /locus_tag="IXF88_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1507722.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="IXF88_000981"
     CDS             complement(4057..5343)
                     /locus_tag="IXF88_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1507723.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="IXF88_000982"
     CDS             5616..6194
                     /locus_tag="IXF88_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1507724.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="IXF88_000983"
     CDS             6194..7123
                     /locus_tag="IXF88_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1507725.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="IXF88_000984"
     CDS             7120..8856
                     /locus_tag="IXF88_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1507726.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IXF88_000985"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IXF88_000985"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1507727.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="IXF88_000986"
     CDS             complement(9752..10261)
                     /locus_tag="IXF88_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1507728.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IXF88_000987"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IXF88_000987"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1507729.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="IXF88_000988"
     CDS             complement(11562..12653)
                     /locus_tag="IXF88_000988"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1507730.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IXF88_000989"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IXF88_000989"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1507731.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IXF88_000990"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IXF88_000990"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1507732.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IXF88_000991"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IXF88_000991"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1507733.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="IXF88_000992"
     CDS             complement(16616..17590)
                     /locus_tag="IXF88_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1507734.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="IXF88_000993"
     CDS             complement(17571..19454)
                     /locus_tag="IXF88_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1507735.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="IXF88_000994"
     CDS             complement(19472..21697)
                     /locus_tag="IXF88_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1507736.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="IXF88_000995"
     CDS             21812..22846
                     /locus_tag="IXF88_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1507737.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="IXF88_000996"
     CDS             complement(22913..23926)
                     /locus_tag="IXF88_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1507738.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAHDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="IXF88_000997"
     CDS             complement(24004..24192)
                     /locus_tag="IXF88_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1507739.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7064 a         5340 c         4397 g         7777 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc gcaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcatg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aactaaaa
//
LOCUS       DACESX010000028        24371 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.028, whole genome shotgun sequence.
ACCESSION   DACESX010000028 DACESX010000000
VERSION     DACESX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.028"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(33..881)
                     /locus_tag="IXF88_000998"
     CDS             complement(33..881)
                     /locus_tag="IXF88_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1507740.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1105..3045)
                     /gene="thrS"
                     /locus_tag="IXF88_000999"
     CDS             complement(1105..3045)
                     /gene="thrS"
                     /locus_tag="IXF88_000999"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1507741.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3382..3561)
                     /locus_tag="IXF88_001000"
     CDS             complement(3382..3561)
                     /locus_tag="IXF88_001000"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1507742.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3608..5431)
                     /gene="cadA"
                     /locus_tag="IXF88_001001"
     CDS             complement(3608..5431)
                     /gene="cadA"
                     /locus_tag="IXF88_001001"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1507743.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5709..7988
                     /locus_tag="IXF88_001002"
     CDS             5709..7988
                     /locus_tag="IXF88_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1507744.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8081..8728)
                     /locus_tag="IXF88_001003"
     CDS             complement(8081..8728)
                     /locus_tag="IXF88_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507745.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8907..9626)
                     /locus_tag="IXF88_001004"
     CDS             complement(8907..9626)
                     /locus_tag="IXF88_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1507746.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9757..10908)
                     /locus_tag="IXF88_001005"
     CDS             complement(9757..10908)
                     /locus_tag="IXF88_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1507747.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11125..11865)
                     /locus_tag="IXF88_001006"
     CDS             complement(11125..11865)
                     /locus_tag="IXF88_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1507748.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11869..12192)
                     /gene="rsfS"
                     /locus_tag="IXF88_001007"
     CDS             complement(11869..12192)
                     /gene="rsfS"
                     /locus_tag="IXF88_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1507749.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12249..12842)
                     /locus_tag="IXF88_001008"
     CDS             complement(12249..12842)
                     /locus_tag="IXF88_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1507750.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12826..13473)
                     /locus_tag="IXF88_001009"
     CDS             complement(12826..13473)
                     /locus_tag="IXF88_001009"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1507751.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13486..13797)
                     /gene="yhbY"
                     /locus_tag="IXF88_001010"
     CDS             complement(13486..13797)
                     /gene="yhbY"
                     /locus_tag="IXF88_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1507752.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13813..14922)
                     /gene="yqeH"
                     /locus_tag="IXF88_001011"
     CDS             complement(13813..14922)
                     /gene="yqeH"
                     /locus_tag="IXF88_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1507753.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14919..15446)
                     /locus_tag="IXF88_001012"
     CDS             complement(14919..15446)
                     /locus_tag="IXF88_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1507754.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15626..16411)
                     /locus_tag="IXF88_001013"
     CDS             complement(15626..16411)
                     /locus_tag="IXF88_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1507755.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16404..17273)
                     /locus_tag="IXF88_001014"
     CDS             complement(16404..17273)
                     /locus_tag="IXF88_001014"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1507756.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17274..18653)
                     /locus_tag="IXF88_001015"
     CDS             complement(17274..18653)
                     /locus_tag="IXF88_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1507757.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18661..19080)
                     /gene="fabZ"
                     /locus_tag="IXF88_001016"
     CDS             complement(18661..19080)
                     /gene="fabZ"
                     /locus_tag="IXF88_001016"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1507758.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19105..19581)
                     /gene="accB"
                     /locus_tag="IXF88_001017"
     CDS             complement(19105..19581)
                     /gene="accB"
                     /locus_tag="IXF88_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1507759.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19584..20819)
                     /gene="fabF"
                     /locus_tag="IXF88_001018"
     CDS             complement(19584..20819)
                     /gene="fabF"
                     /locus_tag="IXF88_001018"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1507760.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20838..21575)
                     /gene="fabG"
                     /locus_tag="IXF88_001019"
     CDS             complement(20838..21575)
                     /gene="fabG"
                     /locus_tag="IXF88_001019"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1507761.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21587..22504)
                     /gene="fabD"
                     /locus_tag="IXF88_001020"
     CDS             complement(21587..22504)
                     /gene="fabD"
                     /locus_tag="IXF88_001020"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1507762.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22611..22835)
                     /locus_tag="IXF88_001021"
     CDS             complement(22611..22835)
                     /locus_tag="IXF88_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1507763.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22888..23850)
                     /locus_tag="IXF88_001022"
     CDS             complement(22888..23850)
                     /locus_tag="IXF88_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1507764.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23860..24300)
                     /locus_tag="IXF88_001023"
     CDS             complement(23860..24300)
                     /locus_tag="IXF88_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1507765.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6670 a         5427 c         4243 g         8031 t
ORIGIN      
        1 ctaaaaaact tatataaaaa atagtaaggt ttttatttcg aatccataaa atattcttgg
       61 attgcttctc gtgttttttt cactttttcg ctaagctttt cgtttttagc catatagaaa
      121 taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg aattagttgc cgcacttcga
      181 ttcgtgattt cttttggtct agatgtttgt acgagtacat cggctatctt gctaagcggg
      241 ttatttccaa attgcgtcaa cgcaatgatt tcgatattca tgcttttcgc cagctcggct
      301 aatgcgacta catctgcact tctacctgag tttgaaacaa gccaaagcac tttctttttc
      361 tcattgctga caagttgcgg gagcaagacg tggatatctt tttcaaaaat gattggctta
      421 ccgacacgag accatttttg cagaatatct tctactacta aagaagaggc gccaacccca
      481 tataaaaaaa cagtctctgc cgattccaaa cactctacga ctttttggat cgtttcttct
      541 tctagaatat ctactgtttc ttttatcgct acttgtgcat tgttaccaag tttgtttttg
      601 atagagtaaa aggactcatt tgcttctata tcactaaaac cttcatcgat tccagtctgt
      661 agttctgttg aaatcgtgat tttgaagtcc gtcagacttt gaaaaccgat ttttttggaa
      721 aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg ctaattcatt agctgtcatt
      781 gtagggacct tctcaatatt accgataatg tagttggcca actttttttc tgctgaagaa
      841 taatcttcca tcatactttc aatacgacta attacgctca taagttcctc caccctttta
      901 atgatagtct atgtaagtat accatatcag acccttattc acggtcgtat actggataaa
      961 aaacaaaaaa acaatccgcc caacagaata tcttatccca tcaatcccac agattctatt
     1021 gagcatctgt gtctttgagg aatacctgtt atctattgtt cggattgctt tctatctact
     1081 acttttttac aggaaattta cctattagtt ttctctgctg taatttttga cttctgcttc
     1141 catagcatca ataaagatat tcaaatcttc tacagctgtt tctttacttc cataacgacg
     1201 tacgttcact gttgcatctt ccaattcctt gtctccaaca actaattggt aagggatctt
     1261 ttgtgtttga gaggcacgta tcttatagcc cattttttca ttacgatcat caacttctac
     1321 acgcaacccc ttcatttgca aacgttcttt gatttcataa gcataatcgc catgtgcatc
     1381 aacagaaaca gggatgattg ttgcttgtat aggtgccaac catgttggaa aagcgccttt
     1441 gtatacttct gttaaataag cgacaaaacg ttccattgta gaaacaattc cgcggtggat
     1501 aactactgga cgatgcgtat tttccccgtc ttctccaaca tatgtcaaat caaagcgctc
     1561 tggcaataag aagtctaatt gtatagttga taatgtttct tctgtaccaa gagctgtttt
     1621 gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg ccttctgctt caaagtaatc
     1681 tagttctaat tcatccatag cggctttcag cattgcttgg gctttttccc acattgcgtc
     1741 atcatcaaaa tatttatctg tattgtttgg atcgcgatag ctcaagcgga aacgataatc
     1801 tgtaatattg aagtcagcat atactgccac catcaattca agcgtacgtt taaattcatc
     1861 tttgatttga tcaggacgaa caaatgtatg accatcattc aacgtcattt ctcgtacacg
     1921 ttgtaaacct gacaaagcac ctgatttttc atagcgatgc atcattccta attcagcgat
     1981 acgaattggt aattcacgat aagaatggat cgtgtttttg tagaccatca tgtgatgagg
     2041 gcagttcatt gggcgaagaa caagcatttc gccatctccc atatccattg gcgggaacat
     2101 gtcttcttga taatgatccc agtgtccaga tgttttgtac aactcaacat ctcccattat
     2161 tggtgtatat acatgttggt aacctagact aacttctttg tctacgatat aacgttcgat
     2221 cgtgcgtcgg attgttgccc cttttggaag ccagaatggc aaaccagaac ctacttctgg
     2281 ggaaaccatg aacaagtcaa gttcttttcc tagtttacga tggtctcttt cttttgcttc
     2341 ttcacggaga cggatatatt ctttcaaagc tttcttgtca aaaaaggctg tcccgtatac
     2401 acgttgcatc atttgattgt ttgagtttcc acgccaataa gcacctgcca ctgataataa
     2461 tttaaatact tgtatacggc cagtggatgg aacatgtggt ccacgacaca aatcgatgaa
     2521 ttcgccttgt tgataagcag taatcacttc ttcttctggc aattcactaa tcagttctac
     2581 tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt tttgaaagaa cacggcgttc
     2641 gatcggcaga ttttctttga cgattttcat catttctgct tcgattgccg gcaaatcttc
     2701 tgcagttact tggttttgtc cattatctgt atcgtagtaa aaccctgaat cgatagctgg
     2761 tccaacgcca aagtggatat ttggatagag gcgacgcatg gcttgggcca tcaaatgagc
     2821 tgctgaatga cggactaagc caagtgcatc ttcatggtct ggtgtgatga tctcaatact
     2881 tgcatcttcc tcgattggac gagtcaaatc aacgagtttt ccgttcactt taccagctaa
     2941 agcttttttt gctaagcttt ttgaaatgct ttcagcaatt gccaatgttg ttgttccaga
     3001 ctcaaattct ttgactgcac catctggaaa agtaattttt atcataatag tttcctcctt
     3061 gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa aaaaatccta gcatctcttg
     3121 aaaagagata ctaggacgaa aagttcgtgg ttccacctaa tgttcaaacg caaatgcgtt
     3181 cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca agggtttcga aagtggtcat
     3241 cctttggttt tctcctggaa aagtttcagc ctagcttttc ctctcttatc gaagactctc
     3301 aaattcagtt ggctttctca tcaatcaatt atgtgtttat ttaaccacta ataaatagaa
     3361 aaatcaagtg ggaaactaaa cttatttttt ccattcccca tttacaccgt atgttccttt
     3421 tttcatctca ttgatgatga catggatgtg ttcttttggt gcctgcgtgt ttttatgaac
     3481 agcttccgtg atatctttca tcatattttc taattgttcc ggactgcgac cttctactaa
     3541 ttcgacatgt acgaatggca taatgctctc tcctctttct gatcgttctt ttttcaactc
     3601 gattctatca tactatgttt gtcttgggta ctgaatcagg aaaaatggct ggatcttttt
     3661 ttgctctcaa ggcactcaaa atagagaccg tatccacgac ttcttgcaaa acagctccga
     3721 ataatgcggg gattagtccc gtacttgcaa ttaacatcaa aaaaatacaa ataaagatgc
     3781 caatcagtac agactgtttc gctattctca tcgtatctct tgaaatcact actgcttttg
     3841 ctacacgcaa gagatcatct ttcaagatga ccgcatctgc actttcacta gcagctgtag
     3901 aaccatgagc ccccattgca ataccgatat ctgcagcagc aagtgctggt gcgtcattca
     3961 cgccgtctcc taccatcgca acaggtcttt gatctttagg gatactttct aaaatggtca
     4021 gtttatcttg aggcaagcta cgggcatgta cagaatctaa ttggactttt tctgcaatcg
     4081 tttgagcaat ggcagatcca tccccggtga tcatcatcaa atttttcaca tcaagttttc
     4141 ttagctcctt gattgtcttt gctgcttctg ggcgaatctg atccgaaaat aggatcgttc
     4201 ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt ggacgtttct atttcttcag
     4261 ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt attgactact gcttggattc
     4321 cttgaccggt cacttctttc agttgactaa caggatacag ttggctgttt ttcacgttgt
     4381 tcactaacga acgagctaag atatggctag attcctgttc tgcactagct gcatacagaa
     4441 gtagctcttc tttttttatt ccattgactg gctgaatcgc ctctacttcc aatatccctt
     4501 tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt acgtgcgagc ttttcaatcg
     4561 ttgttccagt ttttacaacg atcccgtttt tacttgaacg gctcattcct gcaactaaag
     4621 caatgggcgc agctagaatc agtggacaag gtgatgccac taccagtacc tcagcaaatc
     4681 gtaaagggtc ttttgagaac caccaagaaa taccaccgat caaataagca atcaacgtga
     4741 aagggactgc ataacgatca gctaaacgga caaatcgggc aggcttttct tttgattctt
     4801 cgactagttt aataatcatt tgatactggc tgtctgcggc acgtttttca gcgatcattt
     4861 ggatcgatcc gtctccattt aaagaccctg acataatctg atctccaggt ttcttggttt
     4921 ccgggcggga ctcacctgtc aaagaagatt catcgaccga agattctcct ttggtcactc
     4981 gaccatctgc gggtacaagt tcgccaggct tgactagtaa ctggtcatca atatttattt
     5041 catcgattac tatatcctgc aattttcctt ctttgaatcg atgggcaatt cttggggtgt
     5101 tatctaacaa agactgcagt tctttacccg ctcgtttatt cgcgtaatcc tctagactat
     5161 ctccgcccgt aagcatgatc aatatcatca aactagccca gtattctttg acggctaatg
     5221 ttgcaaggat tgcggtaatc gctaatacat ctacgccata tcttcctgaa cgtaacgtct
     5281 tgatcatttc caaaaacatc aagaaggcga gaatccctcc agtaaatgcg acaatcgcaa
     5341 atgcaagggc cggctggtga aaaacaaatt cacttaataa agctatccca ccaataagca
     5401 aagtaagtaa aaattttttg aaatgtgtca ttgttttttc tcctctttta tgtgacttag
     5461 agaggttagt aataaatttc cagctgcctc tttcatctta agcatagtcc ttgactagca
     5521 ctttttaaaa caataggcct taattgaaaa caactatcac tatcagcgtt tccaaaattc
     5581 atcagttcta caaacaaaaa tattcagcaa ggattacttt ttcgattcat cagttaaaaa
     5641 gtaccttttt tcgaaaaact atgctatact tttaattcgt tatttacggc ggaaggagca
     5701 aacatgcgat gaatgagaaa caactagaac aaacgcacgt ggatgaaacg atcgaactaa
     5761 tccagatcga acaaaatatg ctggatacaa aacaacaaga attgactcag gaaatgaatc
     5821 attcgacaaa agacacagct aatcacaaga ttcgagctgg ttccaatgaa gctttttatg
     5881 aatctgctgt tgaatatcgc caacatgagc aggaattgct tttgaaatac catactcttg
     5941 aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa agatagtcct tattttgccc
     6001 gtatcgactt taaagaagac cgtgaaacag aaacattata tctaggtatc gcttctttaa
     6061 gagatctggc agaagaaaca atcgttattg actggcgcgc accaattgcc aatctttatt
     6121 atgaaggtga gattggtccc gcattttatg aaactgaaac agatagaata gatgtcgatc
     6181 tgcagttgaa acgacaattt aaaatccaag aaggacgtat tttatctatg gtagatacgt
     6241 cggaagtaat caatgatgac tttttgctag agatattgga tgaatcatca agcaaccata
     6301 tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc aatcattcgg gacacaacaa
     6361 gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa gacatccgct ttgcttcaac
     6421 gtattgcttt tctgttgtat cataatcgca agtggctcga ggctgaaaac gttcttctat
     6481 tttcacccaa ccatttgttt tctgattaca tctcgactgt attgccttca ttaggtgaaa
     6541 gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca gctgctgcca gagttttctt
     6601 tactagatga acagcaacaa gaaagtggtt ttttgctagg ggaaaaagat ccgatacaag
     6661 tgatgaaaag cggattgacc ttagtcgatc agattgatcg ctatatccaa ggcatcacaa
     6721 actacggtcc cttgttccgt gatatgaaaa taaatggccg tacgatccta tcaaaggaat
     6781 cgattcgcca atggtataaa gaaacaaatg aacaattacc tttacatcat agactttctc
     6841 tattacaaac gaagctcctg aaaaaattag gcggtctgca aaaagacgaa acacgtcagc
     6901 aatgggtcaa agatctggcg gaagaacagc tccaagaact atacgcaaca gatccaaatc
     6961 ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa actggccaac caaatcgtac
     7021 gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta tcaatttgtc aatataacga
     7081 aacaatatct ccactttcta caaactgtgc cactttctct tctaacacga cataaaatca
     7141 gcgagaccag gtggcaggaa catttgacaa ctgttcgtac acacttgcgt caaagagaat
     7201 tgcgtcaaga ggatgctgta ctattctttt tactgatgcg ccgaacatat ccagtgacgg
     7261 ttacacacaa agctcgctat atctttattg atgaaatgca ggatttcgca ccatcacaag
     7321 tggcattgct gcaagccttg ttcccaaaag caaatttgac tttctgtgga gatctaaatc
     7381 aaaatgtctt tggaaatgaa acgatcgttg gatcattgga agaccttttc gtggaccgtg
     7441 aagtcactcg tttccaatta acgacaagct atcgctccac aaaagaaatc actgattttg
     7501 ctaatcattt tattgtgaaa gaacatcaag tagaaacaac agcaagaaaa ggagaccttc
     7561 ctttgctcat ccaaggaaca acagatgata tcaaattgaa ttggttagca gataccattc
     7621 gaacgactca gggaaaagcc cggtattggc gtacagcaat catagctaaa acacaagaag
     7681 aatgcgaagc tctttatgct caactgccag acgagttgaa acaacaagtc cagctgattt
     7741 taaatgagag tgactttatg aagcgttcca ttattttact tccagccttt ttagcaaaag
     7801 gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc agaacatttt tcttctcagc
     7861 aagacaaatt gatcctttac accatgtgta cacgtgcgat gcatgaattg acccttttta
     7921 cctccaataa tgaaagtcct ttgttaaaca atatggatat ttcatcttat gagaaaaaag
     7981 tactgtaagt aaaaagacaa ctgctatcat taatgttagc agttgtcttt ttatttacaa
     8041 taataattgc agaaaaagta attcaatcat ttaaggtcat ttattctata tataaatcca
     8101 aatctgtttc aggatatttt aatttgacaa gcgaaataat cattttcagt tcttccatgt
     8161 tttctggata ttctgggtca aataccttgc tatgacggcc aatcccgcca ttgtcgatca
     8221 atcggtaaat tcgattccat tcaatgaatt ggtcaattga ttggaaattg ctgattggat
     8281 agccgccaca atccaaaatc tctgatacac gaaatgctag gtatgcacta tcgatcactt
     8341 tatttttttt cgtgtctatc ttctcatttc tttcgataag aattttttca ggacgatgtt
     8401 tcaagctcca ctctaatagt agaaagaaag cctccatccg atactcttct tcaaataacc
     8461 gaaaggctga atcagaacga cgaaaaagca gcggaatcag ataattttgt ctataaatca
     8521 gcattggtgc gcggtccacc atacactcta aataacggtt cagataccct gtaccgacat
     8581 agtttcctaa tcgatagcct tcaacgccga atcttgcgta tttttgcgct aattttgaac
     8641 aatcagttac gttcacagct aactcttcta ctttgcggta accgcttaag atacgttcaa
     8701 aaggttctaa agtgattttt gcttccatat tcttccccgt ccatcttcca catacttcat
     8761 atcatttcac atgatactta tgctctcatc ataccacagg ttgaattaga aaagaaaata
     8821 aatcacacaa aaaagcaaga caatctgtta gtgagattgt cttgcttttc gaaaaagact
     8881 gaatactttt cgttaaaact ggttgcttag tcttctaatg ccacattatg gaacacatgc
     8941 tgtacatctt ctagatcttc cagtgcatca atcatttttt caaatgtttc ttgatcttct
     9001 ggagatagtg ttacctcgtt ttgagggatc atttggatct cagcgattga aaaatcagtg
     9061 attccttttt cttttaatgc ttcttgtata ctatggaaat ccgctacttc accgtaaacg
     9121 atgatttgtc cttcttcttc cattacatcg cgaacatcga tgtctttttc cattaggtat
     9181 tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg
     9241 taagaaaccg ctccggaaac acccatgttc ccgccatttt tcccaaatgc tgcccgtacg
     9301 tctgctgctg ttcgattcac attgttcgtc aatgtatcaa caatcaccat tgagccgctt
     9361 ggtccaaatc cttcatagcg caattcagag tatgtttcat ctcctgcacc ttttgctttt
     9421 tcgattgctc ggtcaataat atgttttggt acattataag ttttagcgcg atcaatgacg
     9481 aaacgtagtt tttggtttgc atgcggatca ggatcacctg attttgctgc cgcgtagatt
     9541 tcaatcccaa attttgcata aatccggcta ttattagcat ctttagcagt tttctttgct
     9601 acgatatttg cccatttacg tcccatattt tcacttcgct ttctgatttg atagtttcat
     9661 aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg attctgtgaa gctttttttc
     9721 tttcttcctt cattcttctc atttttagtc aattatctat actcggattg ggatttgccc
     9781 gaaattctgt tccggaatat tgcctcccaa ttgatagatt tgatcacttc taactaacag
     9841 ttccgcttct tgtgcctctt tttttccgat tcgggaataa atagaaatct cttttttttc
     9901 ttttaagaat cttcttccag catcggtaaa tccaagtagt cgaagataat ttttattttg
     9961 ttggacagga atctcttgct ttttgatatt cagcaagaca taacaagata atcgttggat
    10021 ccgtgtccat gtatatcttt ttgtttttac ctttgacaca aaatcctcaa aactatcagc
    10081 agttgcagct gctgttttca tgcgcgattc tattccttcg gtcatctgat agatatctgt
    10141 cagttccagc aaagtagaag ataacaaccg ataacgcaaa tacggccaat aagattccca
    10201 agtctgcagc gaacaagttg aaagatcttc ttctgtctga ggcggcacaa gtccagctat
    10261 tctttttcct tcttctaacg catggcgaat ggctgttgca ctggctatcc tctgatctgt
    10321 gatgattttt tcatgatagc ctgcactttt acgtcggatc ggatgtaatt ccatagggtg
    10381 gacatacttg gcattttcct ttgcataact taatcccaag atatgatttg gcgaagaaaa
    10441 atcaagcgta atatcgggat acagactgcg aaaaacctca gtcatttttt gcggataact
    10501 atacgtttga tcagacagtt cttgaaatgc ctcttctatc ctatcttgat tctcttggac
    10561 aaagcgtccg aaactgctgt aatcaaacga agtctcttct tctgttccaa aacacaaact
    10621 gtcacagtcc agtgtttgca agatttttac tgcaccttta gcaaaatagt cagctgactg
    10681 aaccgcccat tcgacgggaa gctccactac taaatccaca ccattttgta aagcttcatt
    10741 cgcgcgatgc catttatcga tgatagccgg ttcgcctctt tgcaaaaaat tcccgctcat
    10801 tacagcaatg acgacctctg ctcctgttat ttttcgtgcc atttccacat gatagcgatg
    10861 tccattgtga aaaggattgt actctacgat aatcccgcag cttttcatgc gcatcctcct
    10921 tcatagttat cgataaaaaa gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa
    10981 tacccgaact atataaacta ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact
    11041 gcaagagcaa tgcctatatc gttcggatat ataatttatg ttgaagattt attcttttct
    11101 tgttaactcg agcaggcaaa accgctagtc tttgtatgct acaaagaacc agcgctttga
    11161 tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct
    11221 gttttccagc attcttaaat aactttccaa tggataggta cgctcttgat gtagttcatc
    11281 ttcacgaata aatcgaccag acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc
    11341 gatgctgtgt ttctcgcttc ctggataact atcccacaaa aaagcaaaat tatccccttg
    11401 ataatgatag ctatattctg gaaaaacctc atctacttga taggtggagt gtacgtcaaa
    11461 taaaaatcgg ccattttctt ctaacgcttg atagacttca tcaaacactt gctgtacctc
    11521 ttgtctattt ttcatatagc acagcgaatc agaaaaacag gtaattgcct gatattgtcc
    11581 gatttctgat aaatcgagca tattgccttc cacaaattgg atcgatgcgt cttcttcata
    11641 tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg tctaaccctg tcactttata
    11701 tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg catgccagtt ccaaaatatt
    11761 ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac cattgctgat aaagtgtttc
    11821 gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa gccatatgtt aatccaacca
    11881 ttcactaaga tcgactagcg gagcatctga ccatagtttt tccagattat agaaatttcg
    11941 ttctggcgca tggaaaacat gtacgatcac atctccaaga tcgatcaata cccattttcc
    12001 gccttctttt ccttcaatac gttttacttc atatttgttt tcttcttctt tatcaatgat
    12061 ttcttctgta attgcgttga tttgacgttc gctatttgct gaacagatca tgaaataatc
    12121 tgccaacaag gaaacatttc gaacgtctaa agcgataatg tcttctgccc gttttgaatc
    12181 tgctgcttca atcgcaattt ttaattgttc tttactatct atgatgattt cctcctaata
    12241 tgagcagctt atttttttgc tacccagtaa ttatatgttt cgattgtttt tggataaatc
    12301 tgttgttctt gcgcaagcaa atgggccaat gtatgttttg tttcaaatgc tacagcttcg
    12361 tctagatcag cccatgcaat ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt
    12421 tcaatatagt ctgctacata aatgattttg tcaaggagac tcatcttagc agccccagtc
    12481 gtatgcaccc ggattgcatg aagaatctct gcatcggtga ttcctaattc ccgctctaca
    12541 aacgaagcgc caacaagacc atgccagatt gcattattat aattcaataa ctccggatca
    12601 aaaccgtctc ttacgattac cattttgaac tcctcatctg gtcgttcttt tgcataatca
    12661 tgtgtcaatg cagcaatact agctttttca ggagaagcac cgtatttttt ggcaagagcc
    12721 acggctgttt cttctactcc aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt
    12781 attttttgca tcaggttttc tctagaataa gcagtatatt cattactata gtccatattc
    12841 atacaaccct tcattttgaa tataatcaat tactttatct ggaacaaggt agcggataga
    12901 acagccttct ttgatctttt gacgtatttt cgtggaactg atgtcgatct caggtacatc
    12961 tacccaaatt acaggatatg gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa
    13021 gttgaccata gaagtcagct catcgatttt ataccatttt ggcaaatatt ctaccatatc
    13081 cccaccgata ataaagtaat aatctgtatc cggattgttt tgcgtcaatt ccttcatagt
    13141 atcataggtg taacttttcc caccacgagc taattcaatc gtttcaatct gcaaaaaagg
    13201 attgtcttcc accgctaatt ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt
    13261 ctcatcaaca tgaggtggct gataagaagg catcaaaaat acgcggtcaa gacctaaatt
    13321 ccggccagct tgttcagcca tcactaaatg tgcaaggtga acgggattga atgttccgcc
    13381 caagatccct acttgctttt tcttctctaa gaattgaggc atttcttcag gaaataattg
    13441 tgaacgaaca aatgtcttag cttgcgtatt catcatggtc actccttata ttgcttttac
    13501 ttcttttgat agcctttgat acttttcttt tgtggaaggt ttgtataata caataacacg
    13561 tccaatgatt tggacgattt cgcaatctat ttctttttct aatacttgag ctgcttcttc
    13621 tgcaatctca tctgtatttt gaagtaacga tactttgatc agttctcttt tttctaaagc
    13681 ttcttcaatt tgaacgatca ttgctgaatt gatcccacct tttccgattt gaaaaatcgg
    13741 ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt tttcctctta aactcataat
    13801 tttccttctt ccttaaatca atgctttgcg tttcaataca tctacaccct ttggcgccca
    13861 gcccgcaatt actactggcg ctgttacagt gatccagcct agaccggcaa aaacgatgtc
    13921 tgttttttct ttcacagaaa actcaaaacg aacaagctcc ggaaatgcct cgacttcatc
    13981 aggacgagga ggctgtaata atccacctac atgtttttga taaaattcgt ctgctttttc
    14041 cagtttcgtt cgatgaatat ttacatcatt tgacacataa gcaataaatg aagcacgatt
    14101 gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag
    14161 ctgataaact ttaggtttga tttcttttaa cggtgcaatg atccgcaaat cttttttacc
    14221 taagtagtgc gccatttgat ggcgatggat aattcccggt gtatcaatca aaaaatgccc
    14281 gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat
    14341 tacatcttgt actcccgctg tatttttgat gatttgattg ataagcgtcg attttccgac
    14401 atttgttact ccgacgatat agacatcccg tccatcgcgg tattcttcga ttttttctaa
    14461 tagttccgtc atctcatgag atttcttagc actagttaat aatacatcta ctggacgaat
    14521 tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat ttagtttttt tcaatgattt
    14581 aggtaatata tccactttgt tcccaactaa aagtactgga ttgtcaccga caaaacggtg
    14641 tagtcctggg attagtgaac cattgaagtc aaaaatatcc accacattga cgatcaatgc
    14701 atctttttgc cctaattcat tcagtaagcg caaaaagtcg tcatccgtca atgagacatc
    14761 ttggatctca ttataatggc gtaatctgaa acaacgctga caatagactt ccccggaagc
    14821 taaacctttt tccaaagccg tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg
    14881 tataacggca ccacagccga tacattgtaa ttcttctgtc attcgattcc acctcgccag
    14941 cccatatcag ggtgttttgc taatagatag gccataattc ttttttccca aaaacgatta
    15001 aattgtgtct tccaactatc tgttgcgata atgggtttta caaggatgct acgtatccct
    15061 gcacgattgg ctccacgtat atcagtcata atctgatcgc ctaccattac tatctcatca
    15121 tctgctaaat ccaattgttt ctttgctatg ttgatccctc ttgctaaagg tttcattgct
    15181 cgtgcaacat acaaaagatc aaatttttca atcgctcgcg cgacacgttt tgaattattg
    15241 tttgaaacaa caacaacagg gattcctgca tttttcattt ctaaaatcca agtaagcaac
    15301 tcttctgttc catctggatt attccatgca atcaacgtat tgtctaaatc ggtcaatact
    15361 gctttgatgc ctagattctt taattgggca ggtgtaatct gatagatggc ttctaccatc
    15421 catgttggtt tgtaatttga aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac
    15481 ataaagtact cctcaacccc gtattatact gcatatcagc aaaaaaaact acctctatac
    15541 tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac caaaaataaa gattgatcac
    15601 agaaaaattt agaaacaaaa aacaactaat attggcggaa cctcttgtat cgtttttcca
    15661 gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct ttcaatcaac gcttttttca
    15721 taatgtgtgc gactttatat cgtggcaact gctcgccgtt gaacgtttca ggaataactc
    15781 gatcaacgat tcctaactcg cgtagttctg gcgcagtaat tttcatcaac tcagctgctt
    15841 ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa tccttctgga gaaagcacag
    15901 cataaatcgt ttcttccagc atccagactt catccgctaa acctagagca agggcaccgc
    15961 cacttccacc ttcaccaatg atgatggcga tgatcggtac ctttaaatca gacatttcga
    16021 gcagatttct ggcaatagcc tctccttcgc cgcgctcttc tgctcctaca ccgcaatatg
    16081 cacctgctgt attgatcaag gtaatcaccg gtctattgaa cttttccgct tgtttcatca
    16141 gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc aaaatttctg cggatatttt
    16201 cctgcagatc ccgtcctttt tgaataccga tcactgttac cggtttgcca tctaatcgag
    16261 cgattccacc aacaatcgct tcgtcatctg caaataaacg atcaccatgg cattcctcga
    16321 aagaatcgaa gatctcttca aaaaaatcca agcttgtcca tcgttcttta gctcttgcta
    16381 actgaacgac atcatgtgct gtcttatcca cgatctatcc atcctttctg tgtatgcaat
    16441 tcaagaagcg tatggatttt ctgtttcaat tccatgcggg gaacaatctg atcgacaaaa
    16501 ccatgagata ataagaattc agccttttgg aaatcttctg gaagttcctg cttgatcgtc
    16561 tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt ggggttctgc tagaataata
    16621 tcgccttcca ttgcaaaact ggcagttact cctccggtcg tcgggtccgt taaaacagtt
    16681 agataaaaaa gaccttcatt gctatgccgt ttgacagcag cagagatttt tgccatctgc
    16741 attagagaaa agattccttc ttgcatccgt gcgccacctg aagctgtgaa caagacgact
    16801 ggaagacttt cctttgttgc tcgttcgaat aatctggtga tcttttctcc gacaatcgtt
    16861 cccatacttc ccatgataaa attaggatcc atcactccaa taatgaaggg aatcccctga
    16921 atggttgctt taccagtgag gactgcttca tgaagtcctg ttttatcttg cattttttta
    16981 attttttccg tatattcggg aaatgctagc ggatctttgg ttacaagatc cgtatcccat
    17041 tcttcaaaac ttttttcatc aataatcaaa gctagacgct gccatgcacc gattcgaaaa
    17101 gcgtatccgc aatgagggca tactttttct tcaccgatgt ctttcgtata aaggatatgt
    17161 ttgcaatttg gacactttgc ccacatgtta tcaggaacag atggcgagtt agcaggtttg
    17221 ctctctctat taggattgat ccgaatatat ttctttttct taaacagagc catctatgcg
    17281 cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt cttcttgtaa aaatgctgtg
    17341 ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat ccatctgaaa ttctgcgttc
    17401 gtgatgattc cttctgtgat aaattcactt aaggctcgct gcatcttcat taatgcttca
    17461 aaacgcgtat ttccatggac gattattttt gcaatcattg aatcataata tggcgggatc
    17521 gtatatcccg ggtaaactgc actgtctact cgcagtccca tcccacctgc tggaagcagt
    17581 aagttttgga tcttccctgg agaaggagca aaatggaaag caggattttc tgcattaatc
    17641 cggcattcga ttgcgtgtcc ttggaatact acttcttctt gacacacatt caaaggctca
    17701 ccggctgcta tctccacctg tttttttact aaatcgattc ctgtgaccat ttctgttact
    17761 ggatgttcca cttggattcg agtattcatt tccatgaaat aaaaatcgcc ttcttcatcc
    17821 atcagaaact cgatcgttcc ggcattttca taattgactg ctttagctgc tcgaacagca
    17881 gcgtctccta gtgcttctct ttttgtttgt gagatcacta cagaaggaga ttcttccagt
    17941 actttttgat tgttgcgttg taaagaacaa tctcgctcgc ctaaatgaat gacatgacca
    18001 aatcgatcac ctaagatctg aacttctata tggcgagcag gataaatgat tttttccaaa
    18061 tacatatcat catttccaaa tgccgctgct gcttcttgct gagctgaaga aaaatgctgt
    18121 gggagctctt cttttgattg aactttccga atgcctttcc cgccaccacc tgcagctgct
    18181 ttcagcatca ctggataacc aattctgtct gctatttcca atgcttcttc tactgtagat
    18241 aatacaccag tacttcctgg aatgactggg acatttgctt cttgcatcaa ttgacgcgca
    18301 ttgatcttat ttcccattgc atcgatcgtt gcacttttag gaccaataaa tgtgatattg
    18361 cactcttcac acatggaggc aaatcggcta ttttcagata aaaaaccaaa tcctggatga
    18421 attgcttcgg cttttgtcac gattgcagcg ctcagtactt cctgaacatt cagatatgaa
    18481 tcaacggctt ttgccgggcc aatgcagatt gcttcatctg ctaactgtgt atgaagtgct
    18541 tcggcatctg cttctgaata tatcgctacg gtttggatcc ctagttcgcg gcatgcacga
    18601 atgatacgta cagcgatttc accgcggtta gctatcaata ctttagaaaa catatttcac
    18661 ctatccaatc ataaaggtta attccgcttc ggctactttt ttaccgtcta ctttagcgat
    18721 tcctttgcct attccggctg aagctttcac tttcaatatc tcaacttcaa ggtataaggt
    18781 atctcccggt gtaactttct tacggaattt tgctttgtcc aatccgccga aataagccgt
    18841 ctttcccttg aattgttcaa gagaaagtaa tgcaactgct cctgcttgag ccattgcttc
    18901 aacaatcaat acccctggca taacaggttc ttctggaaaa tgtccttgaa aaaagggctc
    18961 attgatcgtt acatttttct tagcaacgat ccgttctcct tcaaccattt cttccactcg
    19021 atcaatcaaa agcattggat aacgatgtgg aatgatttct tttatttctt gtatgttcat
    19081 tgttttccct ctttcctgct ttgcttattt ttgctgaatc ctaaagagtg gctgtccata
    19141 ttcgacaact gactcgtttt ctacaaggat ttctgtgatc actccgtcgg cctcactagt
    19201 tatctcattc atcagtttca tcgcttcaat aatgcagata acttctcctt tggcaacttg
    19261 atcacctact tttttgaaag ctggtttgtc gggcgctggt tgcagataaa cgattcccac
    19321 gattggcgaa acaacctcga ttccatcaaa tactgccgat tctttttctt tcattatttc
    19381 tggagcagtt gttgcttgtt cagatatgac attgactggc tccgaaactg gcaacgtttc
    19441 gtttgtttga accatattca tgcctcgact tgttttattt ttattcatat acaattcaaa
    19501 ttgaccttca cgcaaatcga attccgttag cgaggattga tcaaattgac tgactaattc
    19561 tctgatttca ttgatatcca aaattattcc tcccagcgtt tcatgcacag aaccgcatta
    19621 tgtccgccaa accctaatga attactcatt gcataagtaa aacttgcagg tttgctttcg
    19681 tttttgacaa ctgtcaagtc aatctcagga tcttgttttt ctacattgat cgtaggtggg
    19741 ataaattgat gttctaatgc cagcaaagtt gccaaggctt cgatcccgcc ggctgctccc
    19801 aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag tatctctgaa attttcacct
    19861 aatgcgattt ggattgcttt tgcttctgcc tggtcattgg ctggtgtact tgttccgtga
    19921 gcattgatat atccgacttg ttccggctgt acaccagctt cttccatcgc aagcttcatt
    19981 gctttagatg caccagaacc atctggagta ggagctgtca tatggtaagc gtcacagttt
    20041 gcaccgtatc caacgatttc tccatagatt ttggcaccgc gagcttgtgc atgttctaaa
    20101 gattcaagaa ctagaacgcc tgctccttca cccatcacaa aaccattccg attttcgtca
    20161 aacggaatcg aaccacgatt aggatcagtt gattctgaga gtgctgttaa agctgcaaat
    20221 ccagcaatcc ccatctcaca aatcgttgct tctgttcctc cagcaagaat gacatcagaa
    20281 tagccatgtt tgatgttacg gaatgcttcc ccaattgaat tcgttccgct agcacaagct
    20341 gttacgatag ctgtacaaat accttttgca cctacacgca aagcaatatt tccagctgcc
    20401 atgttggcaa tcgccattgg tacgaacatt ggagaaactc gttttgcacc tttttcatgt
    20461 agacgaataa cttggttttc gattgttgga agaccaccga tcccagatcc gatgatgacg
    20521 ccaaaccggt cttgattcat tttttcttta tctaaaccgc tcatctccat tgcttctaaa
    20581 gcagcgtaga ttccatagat cgaaaacata tccattcgtt tgctatcttt tttaataaaa
    20641 tatttatcgt aagggaaatc tttgacttca cccgctaagg taatccctgt ttctgaagca
    20701 tcaaatttag tgatcggtgc gatgccattc gtcccctctt ttaagctatt caaaaagctg
    20761 tctgcgtcat ttccaatagg tgaagtgact ccatagccag taattaccac tcgattcatt
    20821 tgtttgctct ccttttctta tccgtgcatg accaatccgc catcgacatt gatgacttgt
    20881 ccggtgatat atggattttg tgctaagaaa actgctgttt gtgcaatatc ttctacttgt
    20941 ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct tgactttgtc tgctaaaact
    21001 tccgtcatat cagttgtgat gaatcctggc gcaatggcat tacatgtaat gcctctagtt
    21061 gcagcttctc gtgccacaga ttttgttaat cctactacgc cagctttact agctgcataa
    21121 tttgcttgac caatatttcc aatcaaccca gatacactag agagattaat gatggcccct
    21181 tctcgctgct tcatcatttt tttcaatacg tgctgcgtca tattgaaggt tccaatcaaa
    21241 ttgatatcca gacatttttt gaaatcttct gcatccattc gcatcacaag tttgtcattt
    21301 gtgattccgg cattgtttac taacacatgg atcgatccaa gcttttcttc tgcttctttg
    21361 atcatttgtc ccgctttctc ataatcagag atgtcaccgg aaactcctac gcattttaca
    21421 ccgaatgctt cgatttcttc tatcttttct tttggaatct ctccgcgacc attcaaaatg
    21481 atatttgctc cagcttttgc aaaagctaag gccatcgctt tgccgattcc tcttgtacta
    21541 cctgtgataa atacattttt tccttttaca tccataacgt tcctccttat agtattgcta
    21601 ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac tggtattgtt ttatcgattt
    21661 ttttcataaa tccgcttaat acttttccgg ggccgacttc gatgacttgt tcgatgccga
    21721 ttgtttttaa cttatggata ctctcataga aacgaacagg ttgcatgact tgctgttcaa
    21781 gtagtcccgc gattgtttct ttcttcatta tttccgttgt tgtgttgctt atgataggaa
    21841 aagaaggttc agagaattga atctgactta ggtccttcgc cagttttttt gctgccggtt
    21901 ctaaaatagc tgtatgaaaa ggcccgctga catttagagg gatcattctt ttgaaccctt
    21961 tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc ttcgccacca atcacgatct
    22021 gctgaggtgt attataattt gcaggagata cgatgccgta cttgctagct tcatgacagc
    22081 tttcctcgat cgtttcaatc ggcgcattca tcactgcaac cattttcccg cttccagctg
    22141 gggtagcttc tgtcatataa gcgcctcttt ttgctactaa cgcaactgct tcagtgaagg
    22201 atagcgcatt actagctact aatgccgaat attctcctaa actcagtcct aaagctgcat
    22261 ctgggatgat tccatgctcc ttcaataagc gataatacgc gatgctgact gttagaatag
    22321 ctggctgtgt atattgcgtc tgatccaaac gttcattttc tgtaaagcaa agttcagcca
    22381 tatcgtagcc gagaatctca cttgcttcat caaatgtctg ctttacaacg gcttcttcat
    22441 ataaatcttt tcccatacct tgatattgtg caccttggcc actaaataag aatgctgttt
    22501 tcatgttttt ctccaatata tccagaatat atgtttgatt cttcctattc aaaaaggccg
    22561 agacattcct atttcaggaa atgtcccagc ccccaaccta attttaatga ttatgcagtt
    22621 tgtttttcta cgtaagtcac taaatcttga accgttttga ttccgtcttc tgtttcgatt
    22681 ttcacgtcaa attcatcttc aatttcattg atgatttgga ataaatccaa gctgtcagct
    22741 tctaagtctt cttggatgtc agtagtcaat ttgatttctt cagtttcttt tcctaattct
    22801 tctgcgatga tttcttgaat tttttcgaat accataataa tatattcctc cgatattgtt
    22861 tgctttttat ttttataatt taacagtcta caactccatg agaatacttc cccatgttaa
    22921 acctccgcca tagcctgtaa atattacttt ctgctgtttt cctaaggtga tctttccact
    22981 agaaactgct tcatccaata agatagggat gcttgcagca gatgtattcc catatttatc
    23041 catattggtc aggaactttt cttgcggcat ctttactttt cgggcaattt tctcaataat
    23101 tctgaggttt gcctgatgca aaagcagata atcaactggt ttgtctttca tgacttcttg
    23161 gatattgttt gcaacgtctc gtacggcaaa atcaaaaata tctctgccag tcatttgcaa
    23221 ataatacgaa ccttctgaat cttctgaata gaacggattt ttattttgct ggtagccagc
    23281 ggtcaatgag ctacttctgg taccgtcagc tgctaatttt tctttcagca attttttctg
    23341 tggattcgct tctaagagaa tcccacctgc tccatcgcca aacaacacag cagtcgtacg
    23401 atcagaccaa tcgattagct tagatagtgt ttcaccacca atcaccagtc ccttagttga
    23461 gccgcagcgg atcatttttt cagccatgct taacgcataa acaaatcctg agcacgcagc
    23521 actgatatca aaagcgatag catgagttgc tcccaatttc ccttgtactt gtgctgctac
    23581 agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa ataaaatcca aatctttagg
    23641 gtttagattt ttctgtttta tcaatttttc cgccactaaa atgcataaat cagaagtctc
    23701 ctgatttgtt acacatcttc gttcacgaat acccgtcctt gacgaaatcc attcatcaga
    23761 ggtatccatc atcttggaaa gatcattatt agtaataatc ttttcaggaa ggacgctggc
    23821 tgttgcagta atcattccaa attgttccat taaatatcct cacttatagt cttgcaagaa
    23881 gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt tcctccgtag ccattccgtc
    23941 taagatgttc ttgaccattt cacgatgaaa atgctgatgg acccgaaata gtaactttcc
    24001 ttttttcgtt agtcccaact ttacaacgcg gcggtcatct tcactgcgga tacgttcgac
    24061 atagcctttt tttactaact tgttaatagc agtggtcaag gtcccaacag taattgatag
    24121 ttctttggct acttctgaag ttgttttttt cttgtacatc ccgattgcct cgatcgtgtg
    24181 catctctgtg attgacagat ctttgaattg agattttttc agctcagact cttcgattgt
    24241 caaaatatca ttgaagacac taacaagata atcatttact gtttttaaat ctggttccat
    24301 gttccaccgt ccactaatta gtttgattat caaatctttt gatgttcaaa ctatatctta
    24361 acttttttca a
//
LOCUS       DACESX010000029        23827 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.029, whole genome shotgun sequence.
ACCESSION   DACESX010000029 DACESX010000000
VERSION     DACESX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.029"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(53..787)
                     /locus_tag="IXF88_001024"
     CDS             complement(53..787)
                     /locus_tag="IXF88_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1507766.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IXF88_001025"
     CDS             1114..1524
                     /locus_tag="IXF88_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1507767.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IXF88_001026"
     CDS             1517..2218
                     /locus_tag="IXF88_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1507768.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IXF88_001027"
     CDS             2218..3804
                     /locus_tag="IXF88_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1507769.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IXF88_001028"
     CDS             3823..4044
                     /locus_tag="IXF88_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1507770.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IXF88_001029"
     CDS             4041..4802
                     /locus_tag="IXF88_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1507771.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IXF88_001030"
     CDS             5182..5691
                     /locus_tag="IXF88_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1507772.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IXF88_001031"
     CDS             complement(5766..6305)
                     /locus_tag="IXF88_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1507773.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IXF88_001032"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IXF88_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1507774.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IXF88_001033"
     CDS             7372..8169
                     /locus_tag="IXF88_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1507775.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IXF88_001034"
     CDS             8197..8832
                     /locus_tag="IXF88_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1507776.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IXF88_001035"
     CDS             8852..9625
                     /locus_tag="IXF88_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1507777.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IXF88_001036"
     CDS             10033..10830
                     /locus_tag="IXF88_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1507778.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IXF88_001037"
     CDS             10808..11221
                     /locus_tag="IXF88_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507779.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IXF88_001038"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IXF88_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1507780.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGLSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IXF88_001039"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IXF88_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1507781.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPVRLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IXF88_001040"
     CDS             15998..16558
                     /locus_tag="IXF88_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1507782.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IXF88_001041"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IXF88_001041"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1507783.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IXF88_001042"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IXF88_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1507784.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IXF88_001043"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IXF88_001043"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1507785.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IXF88_001044"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IXF88_001044"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1507786.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IXF88_001045"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IXF88_001045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1507787.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IXF88_001046"
     CDS             21905..22483
                     /locus_tag="IXF88_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1507788.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IXF88_001047"
     CDS             22535..23017
                     /locus_tag="IXF88_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1507789.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IXF88_001048"
     CDS             23134..23508
                     /locus_tag="IXF88_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507790.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7780 a         4284 c         5116 g         6647 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacta agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagtccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtt
//
LOCUS       DACESX010000030        23749 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.030, whole genome shotgun sequence.
ACCESSION   DACESX010000030 DACESX010000000
VERSION     DACESX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.030"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(12..482)
                     /locus_tag="IXF88_001049"
     CDS             complement(12..482)
                     /locus_tag="IXF88_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1507791.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="IXF88_001050"
     CDS             complement(641..1099)
                     /locus_tag="IXF88_001050"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1507792.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="IXF88_001051"
     CDS             complement(1270..2415)
                     /locus_tag="IXF88_001051"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ1507793.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKRDEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="IXF88_001052"
     CDS             complement(2428..3345)
                     /locus_tag="IXF88_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1507794.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="IXF88_001053"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="IXF88_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1507795.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="IXF88_001054"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="IXF88_001054"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1507796.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="IXF88_001055"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="IXF88_001055"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1507797.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /locus_tag="IXF88_001056"
     CDS             6871..7752
                     /locus_tag="IXF88_001056"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1507798.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /locus_tag="IXF88_001057"
     CDS             7749..8465
                     /locus_tag="IXF88_001057"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1507799.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="IXF88_001058"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="IXF88_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1507800.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="IXF88_001059"
     CDS             9182..10183
                     /locus_tag="IXF88_001059"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1507801.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="IXF88_001060"
     CDS             10180..11541
                     /locus_tag="IXF88_001060"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1507802.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="IXF88_001061"
     CDS             11501..12652
                     /locus_tag="IXF88_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1507803.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="IXF88_001062"
     CDS             12627..13796
                     /locus_tag="IXF88_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1507804.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="IXF88_001063"
     CDS             13971..15350
                     /locus_tag="IXF88_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1507805.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15631..16428
                     /locus_tag="IXF88_001064"
                     /pseudo
     CDS             15631..16428
                     /locus_tag="IXF88_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            16617..17285
                     /locus_tag="IXF88_001065"
     CDS             16617..17285
                     /locus_tag="IXF88_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1507806.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(17485..17871)
                     /locus_tag="IXF88_001066"
     CDS             complement(17485..17871)
                     /locus_tag="IXF88_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507807.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(17843..19225)
                     /locus_tag="IXF88_001067"
     CDS             complement(17843..19225)
                     /locus_tag="IXF88_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1507808.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(19381..20568)
                     /locus_tag="IXF88_001068"
     CDS             complement(19381..20568)
                     /locus_tag="IXF88_001068"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1507809.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(20575..21606)
                     /locus_tag="IXF88_001069"
     CDS             complement(20575..21606)
                     /locus_tag="IXF88_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1507810.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(21622..23076)
                     /locus_tag="IXF88_001070"
     CDS             complement(21622..23076)
                     /locus_tag="IXF88_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1507811.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPTLSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTALLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVASASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            23251..>23749
                     /locus_tag="IXF88_001071"
     CDS             23251..>23749
                     /locus_tag="IXF88_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1507812.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGV"
BASE COUNT         7552 a         4353 c         4897 g         6947 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcatctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagatt atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    15481 caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta
    15541 gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact
    15601 taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca
    15661 gatcaagaga ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt
    15721 ttacggatga ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag
    15781 cgcttgatca agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac
    15841 aattcttatc gaggaaaagt tggagcagta gccaaaaatc ggatccatcg tcgtttctac
    15901 accagtatct gccatcagaa aatcacgacg gatacaactg aatttaaata ccatgaagtg
    15961 gatgccaaag gcattatccg tcaaaagaaa ttgtatctag atccttttat ggatatgttc
    16021 aatagtgaga ttttatccta tcgtatctct gaaaagccca atgcattagc ggtaatggaa
    16081 ggtttggagg aagctatcca gaccactaac gattgtccct atcgtcgtac gtttcactca
    16141 gatcaaggtt gggcgtatca aatgaaagct tatagaaaca aattgaagga gtacaaaatc
    16201 tttcaaagca tgtctcggaa agggaactgc ttagataatt cacttatgga gaattttttt
    16261 ggcttattaa aacaagagat ttttcacggg aaagtttata actgtttcga ggaattaaaa
    16321 tcagccatcg atagctatat ctattactac aataatgaac ggataaaaca gaaattaaac
    16381 tggcaaagtc tcgtccaatt tcgtaaaaca accgtaacgg ttgtgtaaaa atatcatttt
    16441 agagcataaa aaaaaggagt ggatttctcc actccaaaaa agtctaactt ttgggggtca
    16501 ctacagtgtc acagtccttt ttttagtaaa aggatacatt tcttccttgt atattccatg
    16561 cgtgttcgtg agaaagcata tataatttaa ttaacagata gttaggggga gaaaaaatga
    16621 ttttttcaat tacaatgaaa ctggtgttcg ggttgatcgg tttattatta gtggtacgtt
    16681 tgcttgggaa gaaatccatg tcagaaatca caccgtttga tttagtctat acgcttgttt
    16741 taggtgggat cttagaagaa tcaacctatg atgacaatgt ccatgtaggg catgtcctat
    16801 ttgccattgc gctttgggca gtaatgattt atggtatcga acgtatcgtc caaaaaaatg
    16861 aaaaggtcaa tcgttgggtc aaaggcgagc catctgttct aatcaaagac ggcgtcatca
    16921 acatgacaga gttgacaaat aaccatatcg agatggagca gttgcgagca atcttaagac
    16981 agcaagaatg cttttttctt gaaaatgcca aacatgttat cctagagaat gccggtcaaa
    17041 tgagtgtatt gaaaaaatca gatgaagaca aagctttatc gatcctgctt gtagatgaag
    17101 ggcaaattca gcataaagtg cttcagtcca atcaattgac tgaagcatgg cttatggaga
    17161 acttgaagaa agaaggctat gcagatgtaa aacagctgat ttatgttgaa tggtcagaag
    17221 aaaaagggct ctacatacta aataaagaag acgtcatgca tgaatcttac cgaatagatg
    17281 gatagataga cagaggttgt gaaatcagcg ccttggcttg ttactcaaaa ctaagcgact
    17341 ttttcacaac ctctgatatc taatatacgt tgttccagaa gttgcttctt cgaaaaataa
    17401 gccaaaattc cacaaaaatt taagaagcaa ttttcgaaaa atcctttctt attttttgaa
    17461 gttgatcact tctgtcacaa cctctcatgt cacaaaccat ttttttgttt cctttaactc
    17521 aatataccgt acgtcttccc acaatatcgg ctgatggtca atcataaagt aatcttcata
    17581 ggcttcacca atgaagtgcc cttcgacctc atccaagagt cttccaaatt catcttttaa
    17641 attgagctga atgattactg ctttacgaga taaaaaggct tctgtcagta tactatcgat
    17701 ttcttgcaat gtcatttgtt gtttaggaag aggctgtttg cgtgcttctt cttcattttt
    17761 tttgataccg ttgactagtt cttccatcgc aaaagcagtc ccccatttta atccaaatgg
    17821 ccgatctcga tactcgttat agtcaatgaa tttttttgct gtccgtctca tcttttccac
    17881 cttctaatcc actcatacca cctgcatgcc cgccaacgag agaactacgt gcgatggcgc
    17941 gtcctccttg caagagacta tgcgcatgga tgattgattg aaagccgtat ttttttctaa
    18001 tcgtatcgac aacaaaatca actttcgctt catttatctg cttttcagga tcttcaaaaa
    18061 ggttaagctg taaacctgtc gcgtatacga ggtcggaaca gttcacgcct acacgccgaa
    18121 tatcctggta gtggtaatgc tggtggaaaa tggtcaataa agcatctgct agatctctgc
    18181 tagcatttgt cgcttcgatt ttcatttgct ggtgaaaccc ggtcttgcca tttggatcaa
    18241 gatagcctaa agagtatcct acccataagc tgactacttg tgtttttgct gcagatcgtc
    18301 tcaagcgagt gccgacttgg tcggccattt ctttgataac gatctcgatt tcttctcgtc
    18361 ttgtataatc tctatctagg atttgactat ttccgatact ttttgatttt ggtgtatatt
    18421 cttgtcccaa aaaacttcga tcgatgcccc aagcatgggc ataaagctga gtaccgatga
    18481 cccctagctt gcttttcaag tgataataat ccgcgtgagc taaatcataa acagaatgta
    18541 tacccatttg tttcagacgt tttgctgttc gtttgccgat tccccaaaag tcagtgatat
    18601 ctgagatttt ccataacttt tctggtacat cttcataacg ccattcagca aacatttgct
    18661 ttccgtcttt tgagccattg tctaaggcta gtttagcaag tagcgggtta tcgccaattc
    18721 ctatagtcgt gtagattccg gtctgctcaa acacatcttt atggatcagt ttagccatgt
    18781 cgtacgcatt tgttttacca aagagtgcca tgctatttgt tacatcaagg aagctttcat
    18841 cgacggaata gacatgatgg ttcgcttcgt ctgcatattt tttatagata gaattgattt
    18901 ctctgttctt ttccatataa taggccatcc ttggggcaac aatatataaa tcacttggat
    18961 aggggaaggg aagatctctg gcacggctga tattggtgat gccgtaagct ttttttgctg
    19021 caggactgga agccaagatt aatccgctac cccgtgtttc tgtcgaatcg gacggatagg
    19081 acatgactac taatttagtt gtaaggggat caagatcctt ttcaacgcac tctacggagg
    19141 cgtaaaaaga tttgcagtca atacataaaa tatctcttga aggttctttt tcataaacaa
    19201 ataccggatt ttgattcgcc cacataacaa tcacctcttc aacacaatta gaacacacgt
    19261 tctctttccg tgtcaataaa aaacagcaat tttcaaatac gagcaaaagg ctcacatttg
    19321 gaaattgctg agaggttatt tttatctatt tgtttttagc tgagatcgtt gattttttta
    19381 tcaaacatga acagcttctg gatcgatctt tgttgtccca tttccaatgt gtgcaacata
    19441 aaattccgga gcatagccga caactttcat gtattcatca tagacattgt ttttgaaggc
    19501 aggtatattt tcttctttga caagagcaat cgcacagcca ccaaatccag ctcctgtcat
    19561 tcgtgcacct aagacacctt cttgtttttg agcagcatct actagtgtat ctaattcgat
    19621 cccagttact tcataatcat agcgaagaga gtggtgagaa tcatttaaca gttgaccaaa
    19681 tgcttctaag ttcccagctt ctaattcagc ttttgccttc aaagtccgct gattttcaga
    19741 aacggcatgg cgagctctgc gtatcaatgt atcatcgccg atttggtcaa tagttgattc
    19801 aaattcttct tcatctaagt ctcctaaaga agagataggc aaagtttttt gcaaacgtgc
    19861 caaggcttct tcgcattcgc tacggcgttc attgtatttt gaatctgcca attcccgccg
    19921 cttattcgta ttcatgatga cgattgcata accatctagt ttcactggaa ccatttcgta
    19981 tttcagcgta tttgtatcta ataagattgc atgatctagc tcaccgaagc cgatggcaaa
    20041 ctgatccatg atacctgaat tgacaccgat gaattcattt tctacttttt tacctgtttg
    20101 gactagttcc aagcgatctg tttttaaatc aaacagatct tctaatacaa cacctactaa
    20161 taattctaat gaagcgctac tggaaagtcc tgcaccatta gggattgttc cttccactaa
    20221 taactcaaac ccatgatcga ttgagtgtcc agcttctttt aatttaagga ccattccttt
    20281 tacgtaattt gcccagtttg cttttttatc atattctagc tcatctaaag taaaactaat
    20341 gacaccgata tcgtcaaagt ttgtggaata aactaatatt ttattatcgt ctcgaggtgc
    20401 tgctacacct tgcgttccat aagtgatgga agcggggaag acatggcctc cattgtaatc
    20461 tgtgtgttca ccaatcaaat tgatgcgtcc tggtgagaag tattgtgtag tttttttagg
    20521 tccgaatgtt tcggcaaatt ttgtgtttaa gcttcttttg atttccatga tgagttattc
    20581 ctttcttgtt ttgattttga attctgtcac tgtttcaaat actgatccgg cttttaaatg
    20641 aatcgaacca aatgctgaga agcgctctgc acctggagct gtttgagtct caaatgtgat
    20701 gccaccgtga tgtgccagtt ttcggttgcg catttctggt gtttctgtgc cgaaatttgc
    20761 agtgaaaata accacactag aagcatctgt agcaatatct acttggattc ttttatcggg
    20821 gctagtcaag cgagcggctt ctttacccaa gccggtttct tttaagaaaa atggatgatc
    20881 gattccgtca acaagatttt tttgatccaa atcactagca aaaacggacg ctaatttttt
    20941 aggtatttga aaatcgaaag cacttcctgc agcattttct tttaccccta taggaatact
    21001 atctgttcgc aaaggagcat aagcttcgct attgagccaa agctcatgtt gatcgattga
    21061 ctgagaagca tctcccgtca agttgaagta aacatgattt gtcggattga ataaagtatc
    21121 ttggtcactg gtgccgcgag aggtcactcg ccagatatta tcttttgtca aagtatagcg
    21181 gatttccaca tccattgtac ctggaaaacc atgttctccg tcgggacttg tagtagtaaa
    21241 aatgacagaa gcttcatttt cgccattcaa aatgacataa gaccattttt ttaattcaaa
    21301 tccaggagca ccaccatgca gattatgtcc agtttttgga tctgtggcta attggtaagt
    21361 ttttccattg agagaaaagt ggccattttc gattctccct gcggtgcgac cgatagaagc
    21421 accgatatac ggatcttttt cgatatattc ttcagcggtg tcaaatccca aaactaattc
    21481 tcgatcattt gattttaaac tgacgattct agcacctaaa tcagagatag aaagtgctag
    21541 cttgtttttg ttctccaagg ttatcaagtg ataaccttgg ccaaaatctt tttctgtaat
    21601 cttcattttc aagatccttt cttaatggga aggaactact gcatcgtgaa gttcatcttc
    21661 cattgtatca gctggagcca atttgttttc caatttctca acgatttgtg catgtttttt
    21721 ctctgtcaat gtaatcttcc aagcaaaaat aatagcggag aaaatcatca aagcagcagg
    21781 agcatagaat gcataagctt tgaaagtcgt tacgccatga gcagtgatat cagcagcagt
    21841 agcattacca gtcatcccag aagcgctagc gacaaaccct acgatcccgt tagacaaagc
    21901 accagcgatt ttatctagta aagggcgaat tgatagagta acagcttcgt tacggatacc
    21961 atttttccat tgaccatatt caacagagtc tgtgatcgtc atcaaagcgg ataagaaaat
    22021 caattgttga gggaagtaga acaatactag cccgatgata acgattggca aacttgaacc
    22081 tgcgatcgta aagaaaaagt aagaagaaat catcattgcg atccctccaa ggtaaacgta
    22141 gcgacgagag atgacgcgtg taagcattgg gaatagtgga acagctacta aaccaagtaa
    22201 ggcagcaact agaccaacga tccaaaattc agttggttgg ccaagtacat atttgaagaa
    22261 gtagaataat aaggcagttg ttgctacatt ggcaatcgca taagcgatat aagcaagggc
    22321 aagccacatc aactgatcat tttgagcaac tgctttgaac acatctttga tcccagcttt
    22381 ttcattttgc tgacggatcg cagtatcgac ttcttttgtg cccaaggcag tgataatcgc
    22441 agtaccgcct gaaataacag cagcaatcac accgaaccag aaccagcctg aagcgccttc
    22501 aatatgttta tgagtaacaa gataggtaaa gaaagtaacg atagggacaa cgactagagt
    22561 agtaccattt gcacctagag aagagccgaa acgagcaaac gtagcaaatt tttcacgttc
    22621 ctttgtttcc gtactcaaag ttggaatcat tgaccaaaaa gcgatatcct taaatgaata
    22681 aaaactatcc aataagatga aaacgattgc aaataatatg ataaataaag tcgtattatg
    22741 agtagctaag ccaaagaaat tggtaaagat tgctaccaag agtacggaac taacaacgcc
    22801 gccgataact aaccatggtt tgaatttacc gtattttgta cgagtattat cgatgatgcc
    22861 accaatcaaa ggatcaaaaa taatttcaac taagcggata cctacaacta agcttgtaac
    22921 tgtagcaatc atttttgcat tcgttgcctt atctgttcct gcgaacatct ggcttgtcac
    22981 gaataccata aaataagtgc ttaatgtaac atagaatgtg tcgtgtccaa aagcgcctaa
    23041 agcgtaggac acacgttgga tcattttgct tttcataatc tgtattcctc tcctgttcaa
    23101 tatataatac taagtaaacc ggttacaacg aataacaaag atcttttaat gacttaaact
    23161 taacaagaaa tcgcttcctt tttaatatat ttctgtgact tttatttaac tttttgttgc
    23221 attatactaa aggagtaagg agggatagac gtggagcaat cgattttttt aaaaaaggaa
    23281 catcaacaag tagaaaaaga attgtatttt gatttttgtg gtttctctaa aacgatgccg
    23341 tttcattctt ttggtcctgc tattcgcaat gattacatcc tgcatgtcgt gatggaaggc
    23401 aaaggatctt atcatgtgaa aaatcaacaa ttccaattga aaaaaggtga tctgttcttg
    23461 attcgtccag gagactcgac gttttactta tcagatggag aagatccgtg gatgtattgc
    23521 tggctttcat ttggtgggag tgctgcagaa gaaatcattc ggcagtcgct tttcagagaa
    23581 gaccaataca cgatggtctc ttctgacata caaaaatata tcgacattat tttagaatgc
    23641 atgaaagata cggaagaaac cttggaaaat gaattacagt taacagcatt aacctaccgt
    23701 tttttatcag tccttttaaa agatggcgga aaggtcagcg gtggtgtac
//
LOCUS       DACESX010000031        23573 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.031, whole genome shotgun sequence.
ACCESSION   DACESX010000031 DACESX010000000
VERSION     DACESX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.031"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IXF88_001072"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IXF88_001072"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1507813.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IXF88_001073"
     CDS             complement(1174..1869)
                     /locus_tag="IXF88_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1507814.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IXF88_001074"
     CDS             complement(1882..2598)
                     /locus_tag="IXF88_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1507815.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="IXF88_001075"
     CDS             2928..3533
                     /locus_tag="IXF88_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1507816.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="IXF88_001076"
     CDS             3858..4181
                     /locus_tag="IXF88_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1507817.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="IXF88_001077"
     CDS             4363..5733
                     /locus_tag="IXF88_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1507818.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRIFQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="IXF88_001078"
     CDS             complement(5863..7164)
                     /locus_tag="IXF88_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1507819.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9385)
                     /locus_tag="IXF88_001079"
     CDS             complement(7394..9385)
                     /locus_tag="IXF88_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1507820.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="IXF88_001080"
     CDS             complement(9529..9795)
                     /locus_tag="IXF88_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1507821.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IXF88_001081"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IXF88_001081"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1507822.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IXF88_001082"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IXF88_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1507823.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /locus_tag="IXF88_001083"
     CDS             13139..13702
                     /locus_tag="IXF88_001083"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1507824.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="IXF88_001084"
     CDS             complement(13955..14476)
                     /locus_tag="IXF88_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1507825.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="IXF88_001085"
     CDS             complement(14479..15321)
                     /locus_tag="IXF88_001085"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1507826.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="IXF88_001086"
     CDS             complement(15314..16039)
                     /locus_tag="IXF88_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1507827.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="IXF88_001087"
     CDS             16215..16511
                     /locus_tag="IXF88_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507828.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17079..18671
                     /locus_tag="IXF88_001088"
     CDS             17079..18671
                     /locus_tag="IXF88_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1507829.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18684..19424
                     /locus_tag="IXF88_001089"
     CDS             18684..19424
                     /locus_tag="IXF88_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507830.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19592..20941
                     /locus_tag="IXF88_001090"
     CDS             19592..20941
                     /locus_tag="IXF88_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1507831.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21069..21659)
                     /locus_tag="IXF88_001091"
     CDS             complement(21069..21659)
                     /locus_tag="IXF88_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1507832.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21806..23158
                     /gene="brnQ"
                     /locus_tag="IXF88_001092"
     CDS             21806..23158
                     /gene="brnQ"
                     /locus_tag="IXF88_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1507833.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6750 a         4746 c         4491 g         7586 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatctttc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcgg atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgac acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacaggaaa ttttctgttc tagttctttt
    16561 tttattgtat tacatacatt tttgtgtata attatactat cgtttgcaat ggatagatga
    16621 ttcacatttt gattgttttt tcggtaaaag taaagatttc tatatttatc agtatttttt
    16681 caaaaaccag cagttataga ttgacaaagc ctctcgagta tcgtaaactt tttttgtata
    16741 aataactgaa tacgagatgc ctcaatcaat gaggtagagg tcgcgagaat cattaaccct
    16801 gtggagtgaa gcaaacatgg agaagcagga atagggagga tcgccgaaat gtgcactttt
    16861 ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc ggactgtctt agcagtgatg
    16921 ctaagttgcg ctatgttttg tgagaggttt atcaccttat ggaattttag cggccttttt
    16981 atatctataa atcggtcgct ttttattttt tcagataaag ctttaccgca tttcatctag
    17041 accgctcgtg tatcatataa tcagaaaggg agaaaaaaat gaaaaaaaat atggtttcag
    17101 ttgccgtact atttgctttt gtcttctcac tattcagtgt gggatctctg gcaaatgcag
    17161 atgaaaaaac accgagcgat gagtttcgcg taggtatgga ggctgggtat gcacccttca
    17221 actggtctca acaaacagat gagaataatg ccttgccaat tcaaggccaa aatagttacg
    17281 ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga tggtttaggg aaaaaattag
    17341 tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct tcaatcaggc aagatcgacg
    17401 cgatcatcgc cggaatgagc cctactgctg aacgacgaaa agaaattgat tttaccgatc
    17461 cttattacga atcacaatta gtagtagtag ttcaaaaaga tagcaaatat gcaaaagcaa
    17521 aaagtttagc agatctttct ggagcaaaga tcactgctca gttaaataca ttccactata
    17581 ctgtgatcga tcagatacca gatgtgcaga aacaacaagc aatggataat ttctcagcaa
    17641 tgagaacagc tttagcttct ggaatgatcg atggttatgt cagcgaacgt ccggaaggtg
    17701 taacggcaac tagtgtgaac aaagacttgg aaatgcttga attcagcggt gcagatggct
    17761 tccagacaaa cccggaagat gttcagattt ctgttggtat gcgtaaaggc gacccggata
    17821 tccaaaaagt aaaccagatt ctttctggca tctctaacga tgaacgaacc aaaattatgg
    17881 accaagcagt caaagatcag cctgctgcaa ctgatagtga agaaaaacct ggcatcattt
    17941 ctgatttcac tacgatttgg aaacaatacg gcaatatgtt cttacgaggt gctggactta
    18001 ccttgttcat cgcattgatc gggacagttg tcggtactac acttggatta ctgattggtg
    18061 tcttccgttc ggtacctgaa tcaggaaatg cactgactcg cttcttccaa aaaatcggaa
    18121 atgcacttct ttctatctat gtcgaagtat tccgcggaac accgatgatg gtacaagcaa
    18181 tggtcatctt ctacggtctg gcactcgctt ttggcatttc attgaaccga acgattgccg
    18241 ctttgtttat tgtctctctc aatactggag cttacatgac agagatcgtt cgtgggggga
    18301 tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc cattggtatg acccatggcc
    18361 aaacgatgag aaaagttgtt attccacaag ttttacggaa tatcttgcca gcaacaggaa
    18421 atgagtttgt tatcaatatc aaagatacag cagttttaag tgtaatcggc gtagctgatt
    18481 tgttcttcca aggaaactct gcttccggtg cgaatttcca attcttccaa acatttacga
    18541 tcgtcgggat catctactta gtcatgacct ttacgatcac cagaatcctg cgggcggtag
    18601 aaaagaaaat cgatggtcct tcagcttata ccaagcttga agaaatcgac aattcaaatt
    18661 tgcaggaata aggaggaaga atcatgaatg aaaaaattat tgaaatcaaa catctaagaa
    18721 aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt aacagtcaat aaaggtgaag
    18781 ttgtgacgat catcggttct tccggttctg gtaaatctac tttcttgcgc tgtatcaatt
    18841 tactggaaaa accaacaggc ggcgagatca tttataatgg agaaaatgta ttagctccta
    18901 aatataattt acctaaatat cgaacgaatc taggtatggt tttccaatcc ttcaacctgt
    18961 tcaacaatat gaatgtcttg gaaaactgta tgtctggtca aacaactgtc ctgaaacgag
    19021 ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga aaaagttggt atggatcgtt
    19081 atatcgaagc caaaccagca caattatctg gtggacagaa acagcgtgtc gcaattgctc
    19141 gggcattatc aatgaatcca gatgtattgc tattcgacga acctacttcg gcgttagacc
    19201 ctgaaatggt tggcgaagtg cttaatacga tcaaagactt agcacatact ggcttgacaa
    19261 tgcttatcgt gacgcatgag atggaatttg caaaagatgt atctgaccgt gtcattttta
    19321 tggataaagg tgtgattgca gaagaaggta caccagaaga tatctttgtc catccgaaag
    19381 aagaacgtac aaaagaattc ctttcacgta ttttaaatgc ttaggatcaa tagctgattg
    19441 ggacaaagag ctgcggtgtt taccgcagtt cttttttatg aattactcat aaaaggtaaa
    19501 tttctgaaaa ttttggaatc ttagttgtgt ttttcagaaa actctcttaa tatggaagta
    19561 attaaacttt tttagttagg atgatttaaa aatgaatgaa gagcaagaac tgcagcgtgg
    19621 actgaaaaat agacatgtcc aactgatttc tattggcgga gcgattggaa cggggttgtt
    19681 tttaggttcc ggaaagtcta tccagctagc tggcccttcg atattgctcg cctatcttat
    19741 tactggatgc atctgttttt tcattatgcg tgcgttaggc gaacttctgt tatcaaacac
    19801 caataaccat tcttttctgg attttgttgc agaatatctt ggcaaaaaag cggctttcat
    19861 cactggctgg acgtattggt tctgctgggt atcgattgca atggccgatt taacagccat
    19921 cggaatgtat gtacgctact gggcgcctgg tgttcctcag tggcttcctg aactgattgc
    19981 attaggtctt cttctttgct tgaatatggt ggctgtctca ttgtttggtg agttagagtt
    20041 ttggtttgca ttgatcaaag ttgtagcgat cattgccttt atcctcgtag gagcttatat
    20101 gatcctcacc cactatacga cagatattgg cacagccagt atcacgaatc tatggtctca
    20161 tggcggattc tttccaactg gtgcaaaagg atttatctta gctttccaaa tggtaacctt
    20221 tgcttttgct gggatagaat tagttggatt agtcgctgga gaaacagaaa atcctgaaaa
    20281 agtgttgccg gaagctatca ataatattcc tatcaggatc atcttatttt acttaggatc
    20341 tctttttgtc attatggcaa tctatccatg gaacagcttg aatccagata acagtccatt
    20401 tgtagaagta ttttcggaaa ttgggatcac gattgctgct tccttgatca atttagtcgt
    20461 attaagtgca gcagcttctg cctgcaacag tgcaatatat agtacaggac gtatgcttcg
    20521 ttctttagcg caagaaggca gtgcccctaa aaaattcaaa aagttaacaa cacatcatgt
    20581 gccagggaat gctttgacat tttctactat cgtcattttc atttctgtta tcttgaatta
    20641 cgtgatgcct agtgaagtat tcacactagt aagcagtatt gccactactt gtttcttgtt
    20701 tatctggagt atgcttgtct acacgcacat gaaatatcgt aaaagtatcc tcggcaaaaa
    20761 agctcattca tttaaaatgc cgttgtaccc atttagtaat tatctggtat ttctttacat
    20821 ggcctttgtg tgtgtcgtct tgttcctagg aaaagatacg agaatcgctt tattactaac
    20881 acctgtgtgg ttcttgcttc tattactaat ctatcatatg aaatatgaac gtaagaagta
    20941 agaaagtagt tgtgacagaa gtggtcagct tcgagaaata agaaggaatt tacgaaaatt
    21001 gcttcttaaa tttttgtgaa atttcggctt atttcttgga gataaaagct tctgtcccag
    21061 cctcttttct atttccccgg aaacaaccgc tgataatagg catctgcttt tttataggaa
    21121 aaaatccaaa taccagatac aaaaaaacgt ccgaataacc cgatcaacgg tacaaaaaag
    21181 acaccggcaa tcaagcataa gttaatcatc atggagataa gacttcgacg gtcctttgac
    21241 aaaactctca cgacatggga atctggttta tttgctttca tgatttgcct catcaagata
    21301 ccaaaagaaa aactccacat cgtgtatact aagacataag ccatctcagg ccaaaaagct
    21361 tcagggttac gcccgaccca actagtaaca gaaggacata aagtcagcca aaagagcaaa
    21421 aagatattcg accagaccac tcggtaatta attcgttctg ccatctgaaa tatacgatga
    21481 tggttcaccc agatcaccgc aacaaatata aagctgatgg tataagctaa taatgtattt
    21541 cggatgaccc ataaagaatg aaagctagat gtattaggga tcttgatctc taatacaaga
    21601 actctcaaaa caatggctac cactccatca gtaaatgctt ctaatctcga ctttgtcacg
    21661 atgattcctc ctcagatgat tcagcgcttt tattttatca cttacgttat taaataacca
    21721 tcgttttata tagggaattt tctgaaaatt gttgcatatt tttcgaaatt ttgttacttt
    21781 taaaaaaacc gtaaaggagt tatgtatgac aaaaaaatta tcatttaaag actatctttt
    21841 tataggctcc atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca
    21901 tttgggtcaa gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg
    21961 tatcggtctt cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata
    22021 tgaattagca aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt
    22081 agttatcgga ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt
    22141 agcaccattt atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt
    22201 ttttgtgctg gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa
    22261 gttcctgaat cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa
    22321 tccactaggt tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt
    22381 cacagggttt actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat
    22441 catcattgtc acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga
    22501 cacgatcaaa tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc
    22561 ttatttaggt gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct
    22621 ggctcagatt gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt
    22681 tatattagct tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac
    22741 agatttgttt ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg
    22801 tttagcggca aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt
    22861 catctatcca ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca
    22921 gcgtacggct gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg
    22981 gttgaacgct tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt
    23041 tgcggaaagc tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat
    23101 cggttttgtt atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata
    23161 aaagaggttg tgaaagagac gttcagcctt gagcaataaa aagtaatttt gaaaaatggc
    23221 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgttt
    23281 attgagaggt cgtgaaaaag ccgcttagtt tcaagtatta agaaatattt tgtgaaaatg
    23341 gcttctcaca tttttgcata aatttgactt aataccgaga aactggcttt tgaacaccgt
    23401 ttattgagcg tctataaact gacctgagta ttagagaaac aaaacggcaa agtagctcca
    23461 tatactttgc cgttttgttt tgaactaata ccgaagagaa gctgatcaaa caacatttct
    23521 tttataatat agattttaga catatatatg atataaaaat agttttcgca aaa
//
LOCUS       DACESX010000032        23529 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.032, whole genome shotgun sequence.
ACCESSION   DACESX010000032 DACESX010000000
VERSION     DACESX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.032"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            6..305
                     /locus_tag="IXF88_001093"
     CDS             6..305
                     /locus_tag="IXF88_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1507834.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IXF88_001094"
     CDS             378..1739
                     /locus_tag="IXF88_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1507835.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IXF88_001095"
     CDS             1761..2021
                     /locus_tag="IXF88_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507836.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IXF88_001096"
     CDS             2035..2787
                     /locus_tag="IXF88_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1507837.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IXF88_001097"
     CDS             complement(2881..3486)
                     /locus_tag="IXF88_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1507838.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IXF88_001098"
     CDS             complement(3539..4513)
                     /locus_tag="IXF88_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1507839.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IXF88_001099"
     CDS             complement(4891..5670)
                     /locus_tag="IXF88_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1507840.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXF88_001100"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXF88_001100"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1507841.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IXF88_001101"
     CDS             7161..8558
                     /locus_tag="IXF88_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1507842.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IXF88_001102"
     CDS             8639..9079
                     /locus_tag="IXF88_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1507843.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IXF88_001103"
     CDS             9227..9553
                     /locus_tag="IXF88_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1507844.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IXF88_001104"
     CDS             9554..10303
                     /locus_tag="IXF88_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507845.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IXF88_001105"
     CDS             complement(10383..10838)
                     /locus_tag="IXF88_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1507846.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IXF88_001106"
     CDS             complement(11265..11540)
                     /locus_tag="IXF88_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507847.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IXF88_001107"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IXF88_001107"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1507848.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IXF88_001108"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IXF88_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1507849.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IXF88_001109"
     CDS             14770..17436
                     /locus_tag="IXF88_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1507850.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="IXF88_001110"
     CDS             17800..18552
                     /locus_tag="IXF88_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1507851.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="IXF88_001111"
     CDS             complement(18574..19728)
                     /locus_tag="IXF88_001111"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1507852.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="IXF88_001112"
     CDS             19913..22354
                     /locus_tag="IXF88_001112"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1507853.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22684..23388)
                     /locus_tag="IXF88_001113"
     CDS             complement(22684..23388)
                     /locus_tag="IXF88_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507854.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTAENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         7520 a         4127 c         4873 g         7009 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaaatta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaat atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagg cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa aggaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca
    22501 agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg
    22561 acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga
    22621 aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa
    22681 tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa
    22741 tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagcagtcat
    22801 agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg
    22861 ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa
    22921 acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt
    22981 ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg
    23041 cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag
    23101 aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc tctttaatac
    23161 agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc
    23221 taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc
    23281 ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa
    23341 tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct
    23401 tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa
    23461 aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag
    23521 aaaaataaa
//
LOCUS       DACESX010000033        23487 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.033, whole genome shotgun sequence.
ACCESSION   DACESX010000033 DACESX010000000
VERSION     DACESX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.033"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(29..1186)
                     /locus_tag="IXF88_001114"
     CDS             complement(29..1186)
                     /locus_tag="IXF88_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1507855.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(1220..2590)
                     /gene="radA"
                     /locus_tag="IXF88_001115"
     CDS             complement(1220..2590)
                     /gene="radA"
                     /locus_tag="IXF88_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1507856.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(2653..3180)
                     /locus_tag="IXF88_001116"
     CDS             complement(2653..3180)
                     /locus_tag="IXF88_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1507857.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            3410..4216
                     /gene="proB"
                     /locus_tag="IXF88_001117"
     CDS             3410..4216
                     /gene="proB"
                     /locus_tag="IXF88_001117"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1507858.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            4213..5460
                     /locus_tag="IXF88_001118"
     CDS             4213..5460
                     /locus_tag="IXF88_001118"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1507859.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            5536..5880
                     /locus_tag="IXF88_001119"
     CDS             5536..5880
                     /locus_tag="IXF88_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507860.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(5997..6323)
                     /locus_tag="IXF88_001120"
     CDS             complement(5997..6323)
                     /locus_tag="IXF88_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507861.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(6696..7897)
                     /locus_tag="IXF88_001121"
                     /pseudo
     CDS             complement(6696..7897)
                     /locus_tag="IXF88_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            8014..8529
                     /locus_tag="IXF88_001122"
     CDS             8014..8529
                     /locus_tag="IXF88_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1507862.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(8728..9174)
                     /locus_tag="IXF88_001123"
     CDS             complement(8728..9174)
                     /locus_tag="IXF88_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1507863.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(9221..10699)
                     /gene="msr"
                     /locus_tag="IXF88_001124"
     CDS             complement(9221..10699)
                     /gene="msr"
                     /locus_tag="IXF88_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1507864.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(11053..11682)
                     /locus_tag="IXF88_001125"
     CDS             complement(11053..11682)
                     /locus_tag="IXF88_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1507865.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(11666..12130)
                     /locus_tag="IXF88_001126"
     CDS             complement(11666..12130)
                     /locus_tag="IXF88_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1507866.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(12617..13885)
                     /locus_tag="IXF88_001127"
     CDS             complement(12617..13885)
                     /locus_tag="IXF88_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1507867.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(14116..14853)
                     /locus_tag="IXF88_001128"
     CDS             complement(14116..14853)
                     /locus_tag="IXF88_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1507868.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(14920..15489)
                     /locus_tag="IXF88_001129"
     CDS             complement(14920..15489)
                     /locus_tag="IXF88_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1507869.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            15617..16489
                     /locus_tag="IXF88_001130"
     CDS             15617..16489
                     /locus_tag="IXF88_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1507870.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            16831..17703
                     /locus_tag="IXF88_001131"
     CDS             16831..17703
                     /locus_tag="IXF88_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1507871.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            17700..19658
                     /locus_tag="IXF88_001132"
     CDS             17700..19658
                     /locus_tag="IXF88_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1507872.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(19909..20760)
                     /locus_tag="IXF88_001133"
     CDS             complement(19909..20760)
                     /locus_tag="IXF88_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1507873.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            20883..21350
                     /locus_tag="IXF88_001134"
     CDS             20883..21350
                     /locus_tag="IXF88_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1507874.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(21613..22095)
                     /locus_tag="IXF88_001135"
     CDS             complement(21613..22095)
                     /locus_tag="IXF88_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1507875.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(22195..23058)
                     /locus_tag="IXF88_001136"
     CDS             complement(22195..23058)
                     /locus_tag="IXF88_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1507876.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6919 a         4912 c         4250 g         7406 t
ORIGIN      
        1 ttccttacca gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct
       61 cttttttatt gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag
      121 cagctgtttg aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc
      181 catcttctac gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg
      241 tcccggcttt tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg
      301 catttgccaa cgcattgata ttaagaacag gtacattttg gaattcagag actttattca
      361 agttatagtc attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc
      421 tgtctacttc actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt
      481 ctttttgcaa ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat
      541 cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc
      601 cttctaagaa tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat
      661 ccaagatttt atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa
      721 cttgactagc tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt
      781 cttcgattcg tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa
      841 tcggcaatac actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa
      901 cacctaaagt caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga
      961 cctcacttag tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca
     1021 aagataataa aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc
     1081 atccgtttga ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc
     1141 ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt
     1201 ttcgacaatc atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta
     1261 ctccaactat ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt
     1321 atacttttgt gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac
     1381 gtctgatttc tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt
     1441 tttctttata gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta
     1501 attttacccc acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc
     1561 ttttttccag cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc
     1621 gtttggcatt tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac
     1681 gtgtcccttc cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta
     1741 agaaaatctg tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc
     1801 caatctcatt cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat
     1861 gttgttcacc ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg
     1921 cgatcgagcc ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag
     1981 cgattttgag tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg
     2041 tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat
     2101 gttcgatggc acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt
     2161 tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata
     2221 catataagac ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg
     2281 atttcccgat acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc
     2341 ctaacacacg gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga
     2401 cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt
     2461 ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc
     2521 gtcccaagaa cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt
     2581 tttttgccat tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt
     2641 atctttaacg ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg
     2701 atcttgatct gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt
     2761 gatcactaca tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc
     2821 ctcattattg taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg
     2881 cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc
     2941 tttgattcca gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt
     3001 tttcaatcca tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg
     3061 aatcacgacc tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg
     3121 cggtagattg atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac
     3181 cggttttcct ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt
     3241 tctttacaaa ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt
     3301 tccaaacgac tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac
     3361 aagtagaata gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga
     3421 aattaaaaat gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa
     3481 tggaaatatc aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa
     3541 tcggggaaag gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt
     3601 acatttgtca gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca
     3661 agccgaactg atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca
     3721 gatcctcgtg acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac
     3781 attggaacag cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc
     3841 agtggatgaa ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat
     3901 ggcaaaactt gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc
     3961 agataatccg actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga
     4021 aaagattctg gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag
     4081 taaattaaaa gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg
     4141 aaaaaatccg gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa
     4201 ggaggacttg aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa
     4261 caactagcac ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc
     4321 atcaaaaaga atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc
     4381 gaaaacaata tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat
     4441 gccatggcta cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat
     4501 gagatgtgga cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg
     4561 accggcatca tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc
     4621 aaatcaggga atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta
     4681 ttagtccaat tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag
     4741 tttgtcgatg atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta
     4801 gatgtgctga ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg
     4861 gtacctatca ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag
     4921 ttatctatgg cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac
     4981 gcggcagaaa cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa
     5041 aaagaactgg tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta
     5101 gagacggctg ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt
     5161 gctgtcaaag tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact
     5221 aaacattcag aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa
     5281 gtggacgcgg cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt
     5341 ggttttggtg cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg
     5401 ttaaatgaat taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa
     5461 tgtttcgtgt gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac
     5521 tagaaaggtg tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg
     5581 aatatcgctg cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga
     5641 agcataggga gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg
     5701 ctggttacac gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat
     5761 tactattctt ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt
     5821 tttcacttat cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa
     5881 aaaatatccg tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt
     5941 agaggctgtg acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac
     6001 cagctgcctg gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg
     6061 ccatcataat aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct
     6121 tccgctgatt cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc
     6181 tgcatcgctt ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca
     6241 cgataaatca accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact
     6301 tcctgaaaat attcttccag catctattat ccccccattc tttttcttaa atgaatttta
     6361 gcttacctac tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa
     6421 aatcgtgtca catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat
     6481 aagtcacaat attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag
     6541 agttaaacga cagtctcaca acctcttggt aaacggcgtt caaaagcttg catctgcggt
     6601 aataagccca accaaacgaa aaatatgaga agctattttt gtttggctgt tcttatgact
     6661 tgatgcagga cagctttttc acaacctctg atttcttatt cttcttcatt tgtagcaaaa
     6721 taattacggt gataaatgat acaacataca ctgataagaa caatggcacc tgcaaaataa
     6781 aacgcatagc tttcaccaat atgttcacca atcacacctg caataaatgg tgctaaagct
     6841 ccaccgaaaa agcggacaaa actatagctg gacgaagcaa cgtttcgttg cagacctggt
     6901 atttccatag caatcgtcgt caacagtgta ttgataattc cttggaaaaa tcctgctaac
     6961 acaatgctga tcggtacaag gactggcata gaagttccag ctcctaataa cagcaaacaa
     7021 ataatgaata agacaaatgc tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga
     7081 gccggtgcaa cgaaaatcga agcaatagcc agaagcacac cccagccaaa aaatacaaaa
     7141 ccgacttcta attcagaata gccaatcaat aaaaatggcg catacgcaag taaagtaaag
     7201 aatccgaaat tatataacaa tgcgattagt ccaactgaac gaagtttatg attgcttaac
     7261 gctttgacac cttcccaaag gcctactttt ctctttggtt tctcagtttc aggtaaaagt
     7321 accgctaggg ctataaaacc gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc
     7381 catgaaccac tgcccaagac cccaccgact agagggccaa cagccattcc gcagccaagt
     7441 gctgcttcat acatcatgat tgctttttct gtgttaccaa ctaaaatact gacgatcgtt
     7501 gctaaagcag tagagacaaa taaagcattc ccgacacccc agccggcacg taacccaatc
     7561 agcattccga tattcccaga taatccgcca agaccagcga atacaacgat gataaacaat
     7621 cctgtcatca atgttttttc aaaccgatcc gactagataa gaaacctgta aatagcatca
     7681 ccacaccagt gactaacatg taactagtaa ataacagggt cgtttcagct ggcgtagcgt
     7741 ttaactgtgc agcaatcgtt tttaagattg ggtctactag acccactccc ataaaagcga
     7801 tcatacaggt agctgctaca actagcgctg aacttttccc ctttgtttct tcatttgttt
     7861 gttcttgtat atctatttga ttctccaata tttccatccc tttctctcta ttagcatcat
     7921 acatgattta ttttacatgt aaatatgcac atgtaaaacg tggaaacttt actgtgatat
     7981 aataaagaaa ataagacgag aggtaggatt tttatgaata ctttagccta tatattatta
     8041 tgcgtgcttt tatccaaacc acgttctggg tatgaattga agcagctgat cacaattttt
     8101 tgggaagccc atcacagtca gatctataca acgcttgcca agttagacca gcagggttat
     8161 gtgaccgttt tggacaaaga cgagcactct caaaaaaaga tctaccactt gactgatgaa
     8221 ggacgtgctc ttgtagagga atggatcaaa gaggaaactc ccgctcctac gcaaaaagat
     8281 gaatttctag caaaaatcta tgctttttcg acgctcgata aaacaacagc tactggactg
     8341 ctattcacta gagaacaaca gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat
     8401 gacctctcgc tagacaagaa acaagaattt ggacgttatg ttgtgatcca gcgcagaatt
     8461 atgctttgtc agcaggaact ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt
     8521 tttgagtaag ggataaacag aacctttcta gaaatatcta ttgaagagca aattatcttc
     8581 aataaaagag caatacagtt ggaaaagaaa aagcgatccg aacgacaggc caaaacctat
     8641 cgttcggatc actatttttc tagaactgca ttcaagagac tatgacaaaa agcatgccaa
     8701 ggcctttttt caaataacag tgttccatca ttcattactt tgaattgctt ttagcacact
     8761 tcttgaaagc gggccaacga tgatcaactg caacggtaaa gcggcaatca cattcatttt
     8821 ccaagcattc atataagcag agaataattg atcaggtatt ccttgttcca tgatcacacc
     8881 gaataaagac atacatgtca ccattcctaa aaccatcaag catgaaatag ttaagatcat
     8941 ttggaccttg ctttctggat ttactggtag tttaaaggca atttttttcg ccagcacacc
     9001 gatgatgaag acatccaaaa tgaaagccac cacgaatcca ggcactagac caatcaacaa
     9061 acttgataaa tgaagatctt gatgcagcca taaattatac gcgctcatcc ccaagaccat
     9121 caaaaaacac atcattgttg taaaaattat tccttctttt ttatttgtag gcaatctgta
     9181 ccactccttt aaatttgtca cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta
     9241 ctttttttat tttctgccct tttatttcat aaacttcatc agccgtacgc tcgacaaagt
     9301 aagaatcatg agaagtaaac aaaatcgttc ccggatatat ttgcattagc ttctctaaag
     9361 cttcgatcgt tgccatatca ataaaattag tcggttcatc taacagcaac acattacttg
     9421 gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc tcccccactt aatgtacata
     9481 acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg
     9541 attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat ggattcttca gatgtcattt
     9601 gataaccctt ttgctgatat acttgaaagc ttactttcgg agagagaagg attccttctc
     9661 cttgtttgcg gatatgttct aataaggttg tcttacctga accattttca ccgacaatcg
     9721 cgattttttt acctaaagaa aactgaaaat caccatttac cagtaatgtt ctcccacttc
     9781 tttccaattg gacattttgt cccatgatgg gatagcggct gtggagttca agagattttg
     9841 gtactggaaa acggatttgt ttcatctgct gcggcttggt tgtttctcct attttttgga
     9901 gtcttctctc taatgttttc gcattctttt ggatggcttt ttgtacactg tctttttgtt
     9961 tagaggaaga caaccgatcc ggtctgattt gttgcttttt ctttttactg gacacttttt
    10021 ccaacttttc cgcttgtttt ttcttttctt ggatcgattt ttccaatcgt tttttctctt
    10081 tcatgatcga ctctgcttct cgtagctgag tctttttctc caattccttt tgagtgagat
    10141 aggcactata attccccgaa aactctcgga tttctccgtc ttttacttcc cagattttcg
    10201 atgctagctc atctagaaaa tagcgatcat gactaacaaa aaccagcgta ccataataat
    10261 aacgaagctc ttcaatcagt cgatcaatac tttttctatc tagatgggtt gttggctcgt
    10321 ctagaagcat cccttgttca taagaagaaa gaagacgtat caactcgatc tttcgttctt
    10381 cccctccgct ccatttccgc tctgaagttt cttccagtct taactggaaa tgactggcta
    10441 attccaaatt taggtccttt gcttcagcga gataggttaa ttgaggcagg tagttgaagg
    10501 tgatctctgt ttggattttc ccatgatcgg gaaaaagttc accggctatt agtttgagca
    10561 aagtcgattt accgacacca ttttctccta tgatccctat acgatcttgc tgatagacac
    10621 gaagagaatc aatagatagt tctagctggt tgccaaagct gacttgtaga tttgttatat
    10681 ttactgctaa attttccata tgttttacac tcctatttca tgtaaaacaa caattatcga
    10741 tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg catcacatct tttcctactc
    10801 tgaacgaaat aactatgact aaatcaccaa atctgtcata gctttcacct attctactgt
    10861 cgttcgaaaa aatggacata ctccatccat gaaattgttg tttatacatg tcttttgaat
    10921 ttatttagtt gttattcaaa agttcgaaac gtaatttcat cgatgcagtc atttttccga
    10981 cctccttttt tattaggtta ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta
    11041 actgaaaacc ttttattcga acgtagtgat cttttctggt gctaaacgaa cagactcgta
    11101 accttcttct accgcttttc ctattgataa aataataact ggaacataac ggtctttatc
    11161 caaaccaaat gcctctgcca actgatctgc ttcgaatcca ccaattggat ttgtttcata
    11221 gccatgatca cgcgcaacta acatgaattg catagccgct aagctagaat cgactttgac
    11281 tacatcgttc atttcttctc ttgaaaaact cttgtaataa ggaatgatcg cagctaattg
    11341 ttgatctctg acttcttttg gcatcttccc tgattcgtaa gcttggttat agatttcttc
    11401 gccatattcg taacattgca tatcgccaaa aatcatgacc attgcagaag aagtatcatt
    11461 ttgtctcgta ttgaaacgga tcaaaggacg caagatttct tttgcttcct cgctttcagc
    11521 gaccacaaag cgccatggtt gcatattgac agaagaaggt gcagtcgtcg cttctttgat
    11581 catttccagc atttcttcgt gtgggatttt gtatgtttca tcatatacgc gaacggatct
    11641 tctaccagtc ataattgttg aaaaatcatt tttttgttcc attattatta acctctttcg
    11701 tttctgttgt tatattgcaa agttgggtat tcaatttatc taaaagtgtt agaaattgtt
    11761 tcatttctac atcgctgaaa gccggggata gcacggaacc tgtatcttta tggtccattt
    11821 cacaatgctc gattttcttc tgggcttttt ctgttagcgc tacatacact tcccggttgt
    11881 tctgaggatt tctctgacgc gaaacatagc catttgcttc caataccttc agatgacgag
    11941 taactgctcc ttgatcaatc tgcaactttt cttgcaaact gctttgtagc aatggatttt
    12001 cttctttcag tctcatcatg atctcgtatc tagtaagact tataccgata ttttcttcaa
    12061 agatctggac gattttttga tcggctaatt tgatttgata taaaaggtga ctaatctctt
    12121 ctaaagacat aatttcctcc tttctaataa ttgactcgtc aacgattgac ttgtcaataa
    12181 tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat
    12241 agaaaagtat agagaaaaga aattttttgt agaatgaacg gaaaaaatga taaagaaagc
    12301 acaacgctaa aaccaagatt attgattctt atcaaactat agaagatagc tcttctgcag
    12361 tagaaaattt tacaccgcca aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat
    12421 attagaaatt ggatcagtat gcttaaacag taataaaaaa gttatgacaa gatgttgtca
    12481 caaccttttt tgagtaaacg gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa
    12541 acaaaaaaca tgaaaatctg tttttgtttg gctgttctta ctacttgatg cagggcagct
    12601 ttttcactac ctctttttaa taaccataaa atcctattcc ttcatatctc cagcccatcg
    12661 gtgttagcca atctctttcc ttagcactca aggtatagtg atgggtacca gttttttcat
    12721 ttggattaaa tagtcgataa attggcttgg aaccaccaga ataaaaactt agaccttcat
    12781 aacgccaacc gatttttgtt aaagagttga tctcagattg tgccattgtg taatgatggt
    12841 ctccactgta aggattgtat aaacggtaga caggttgtcc gctgtttggt gcttgccaag
    12901 caatcccttc atattcccag cctacagttg gcagataatt tttttctccg gcattcaatg
    12961 tatataaatg ttctcctgta tgacggttat acagacgata catcggagaa gttgaagttg
    13021 gtgctgtatc atactgtgtc agattatagg tttcgattac attgttcaat gtagaaccat
    13081 aattaggcgc agtggcataa cgacctgtta gccaagccgt agcatctttg taagagttcg
    13141 tattgctttt ccatgcaccg gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca
    13201 ataccgttac gttatcttgc aatgattctt tgtacgaagg atattttcta aaagcggcat
    13261 tcaccgtaat ccattgccca ttcacatatt ccgaagtagg catcgtaaca gattggccat
    13321 tatagcttcc tttaatccca aatagattgt aattaggggc agaagccaaa gtactgttcc
    13381 cccagccact ttcaacgatg gcttgagcaa tcatgactga tgcatataag tcattggcac
    13441 ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa cgcttgttga gaattagcag
    13501 atgagcgagc ataaaaagca tagccagtat agttttcgaa ttcagacaaa tctgcttgat
    13561 gggtatgtgt caatgtttgc ccatctgtca attggttcgt gatttcttct gcattctctg
    13621 gattgttctg agcttctgct gatgtactgt cttcggtttg tgtttgttct gttgtttcag
    13681 ttgttggttc ttcggttgaa attgtatttt ctgagacagt agctgactgc aaagtactct
    13741 ctgtatcgtt ttcaattcct gtttctgaag aactcgatgt ctgagttgcg acagtttctg
    13801 tcgattcttc agctagcact gttgtgttgg caactgctag atttcctaaa agaataacgc
    13861 taaataataa attctttgct ttcatctttt ttctcctttg aattatgaaa cttttcctaa
    13921 ataacagtgt aactaaaaaa gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt
    13981 tctttttgat aaatttgctt tttctacata aaaagacgta aatgaagaat attcttctcc
    14041 attcacgcct taaattgatg gtcgtttgaa taactattta acaagtaatt ctctttggat
    14101 attatccgtt atggattata cttcctgtgt cgtcggacgg ataatcatgt tactgacaga
    14161 catccggtct ggcgtttcta tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa
    14221 tccccattct ttttgcgctt catgaagttc aagagcagcc tttttgtcgc tgatggtttg
    14281 atataattca gtttgaacag ctcctggaga gatgatcgta gatttgatgt tattttgccg
    14341 ttgctcttga cgtaatcctt ccatgattgc acggacagca aattttgttc cgcaataaac
    14401 ggcggaatca ggataaacaa catgtcccgc cacagaatca gttgaaatga tatgcccaga
    14461 tttttgtttt tccatgattg gaagtacggc ggaaattcca tttaacacac ccatgatgtt
    14521 gatatcaagc atattttgcc attcatcacg gtgaccttca actaatggag cggttggcat
    14581 gattcctgca ttattgtaca atacatcgat tcgaccgaac ttttccatcg ctaaatcgat
    14641 aactgcttgg acttgtgcaa aatctcttac atctgctgtt tgaatatata gctcagcttc
    14701 tggtaaagat tcttttattg cttttaggcg atcttctcgg cgtgcagcta taactagttt
    14761 agcccctttt tctgctaata gcttcgttgt tgcttcaccg attccgcttg aagcgcccat
    14821 gatgacaatc actttttctg ataaatcatt cattctattc tcctcctgtt ttgaaaaatc
    14881 tcttgcttat actcactatc tcttatttca tcttactgtt caaataatcc gttcgacagg
    14941 tttgacgatt ttggcaggga ttcctgcaac gatggtattg tctgggacat ctttcgtaac
    15001 agttgcattc gctgcaacaa tggcattctc gccaatcgtt acgcctggca gaatagttgc
    15061 tcctgctccg atccaagcat ttttcttgat gtggattggc tttactgaca agcctcttcg
    15121 ttcttttgga tcggttagat ggttcaccgt gatgaggttg acacgaggac caatcaatac
    15181 tgaatcttct aaacagatgc cacctaagtc aacgaacatc acttgacgat tgataaagac
    15241 atttttccct atactaatat gtttgccgaa atctgtataa aaaggcagca gaacagtcac
    15301 cgtatcatct actttgtcac taatgatatt ttctagccgt ttgcggatat ctgctggttc
    15361 atggtaccca gtattcagcg ctgctaaagg ctcttcgttt tcagcttgga tctgatggat
    15421 cacttcaaag agcggatccg ttactcgaat ctctttattg acgatttgtt tttcgatgag
    15481 gcttgtcatt ttgctccctc cttttatcct tactttataa cggacggctt atcatgtcta
    15541 atacttataa aacatactta actattcctt ttaactatac ttagtggtat gcttttttaa
    15601 aaacaggagg gaaaaaatgg atattcgtat actgacgtat tttttagcag tagctagaga
    15661 aaaaaatatc agccgagcag caaaatctct gcatatcacg caacctactc tctcaaaaca
    15721 actgaaagat ttagaagaag aacttggtgt gacacttttc actcggggct cccgagagat
    15781 acagttgacg gattctggtc gatatcttta ttcaaaagga aaagaaatct tgtcgttggt
    15841 agaaaaaacg gcgaacaatt taacaaatga cgcaatcatc tctggagaaa tctacattgg
    15901 cggaggcgaa actaaagcaa tctctttcat ttctgatagc cttcattcgt tgatcaaggc
    15961 gcatcctgat atccagtttc atttatatag cgggaatgca gatgatatca tagacaaact
    16021 ggatagcgga ttacttgatt ttggactcgt aatcgaaccg acaaacaaac aaaaatacca
    16081 gtcatttcga ctgccagccc aagatcgttg gggacttttg atacatcgaa atcatccttt
    16141 agcaaagcaa accgtcatcc atccgcagga actgaaaact gttcctttga tcatttcccg
    16201 gcaaacgtca gtggacagtc aactagctga atggctaggt ttcagcatag atcagttatc
    16261 gatcgtcgga acatataact tattgtacaa tgcttctttg atggtcgata acgggacttt
    16321 tggtgcatta gctctagatg ggatcatcaa tacttatggg acggaaaaaa tattcatccc
    16381 actcgatccg ccattacaag cttcactgaa tctcatttgg aaaaaagacc agcctctttc
    16441 tcctgctgct cgtaaatttt tagatgtcct aacggagaaa tacagttaag tagagttttt
    16501 ttccttcgct ttcagctttt taacaacgaa taagataagc aaaagatcca acgattaaat
    16561 aacataagtt tcttaattta tttcttcctt ttatttctga taattccagt aaattactat
    16621 aattataaaa aaagaattga caaaacataa ctgtctatat aaaattgatt acgttaacaa
    16681 taattcgttt catgtgacta gatcatacta ggcatgaaaa atctctgagg aacatggttt
    16741 ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt
    16801 gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat
    16861 gcagtattgg tagatgacgg tggacaggaa aaagtagcta ttggaaaagg gattggattt
    16921 gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg aacggatatt tgtcatggaa
    16981 ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga ttgatgaacg ctttttattt
    17041 gcagcagaaa cgatcattga tcatgcggaa actgttttga tggaaaagtt gaatgaacat
    17101 gtattgatcg ctttgaccga ccacattgct ttttctgcgg agaacattaa taacggtatc
    17161 gtcatccgca acaaactttt aagagaaatc gaagtgcttt acagtgatga attcagtatt
    17221 gctcaatggg cagtagagta tctgacaaaa gagcttggga ttccatatac ttatgacgaa
    17281 gcaggatata tcgcaatcca tattcacagc ggacgaagcg gtcggaacaa taaccatcgc
    17341 agtatccgtg aagtgacgat tgtatctgac atcatccatt tagtggaatc agaattggaa
    17401 acagatatcc attcagaacc attttcactg aattattcaa gactggtgaa tcatttacga
    17461 ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa
    17521 atggtcaaac gaaaataccc agaaagctat aagatttcta aaaaaatccg tgtgttgctg
    17581 accaaagaat accagctcgc gatcacgaaa gaagaactgg gttacttggc catccatatc
    17641 gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat
    17701 tgaaagcata tctacaacga atgggacgtt cccttcaact tccagttgcg gtcttacctg
    17761 ctgctgcttt actagtaggg atcggaaact ggtgggcatc ttatagtaac gacattgtcg
    17821 ctcatttttt acaagctggc ggtaacgcag ttttaggtca attacctttg ctgtttgccg
    17881 ttgggttagc actagggatg tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg
    17941 tcgcatttga agttccagta aatgttttaa aaactgattc agtagctact ttattgaata
    18001 tcaaaccaga atcagttgat ccatcttttg ctcagatcag taacgtattt attggtatta
    18061 ttgccggatt gattgcggca gctctttata atcgtttcca cgaagcaaaa ttaccgatgg
    18121 ctttatcttt ctttagcggc aagcgtctag tgccaatctt ggctgccttt gtcatgttgc
    18181 tgatcagtgc agtgctactg ctcgtatggc ctccggttta ctctgcttta gtttcttttg
    18241 ctaaattcat tttgagttta ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt
    18301 tactgatccc tacaggattg catcaagcaa tgaactccgt gttctggttt gacgtagcag
    18361 gaatcaatga tattggtaaa tttttggctt ctgaaggaac aaaaggcgta acaggaatgt
    18421 atcaagctgg atttttccca atcatgatgt tcgggcttcc agcaggagca tatgctattt
    18481 acagaaatgc acgtccagaa aacaaagcaa aaacagcttc cttgatgcta gcagcagctt
    18541 ttgcttcatt ctttactggt gtgacagaac cacttgaatt ttcatttatg ttcgttgctt
    18601 ggccattata tgtcttgcat gcaattttca ctgggatttc tttagcagtg agtgcattct
    18661 tccattggac agcaggattt gcctttagtg ccggatttgt cgattacttc ctatctttga
    18721 aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca aggattagta acagcagtga
    18781 tttactactt tggctttaac tttgctatca aaaaattcca tctgatgact cctggccgcg
    18841 aggaagcaga tctttcagat gacgatactg caacgactaa ttcagacaat aaatatgcgg
    18901 cacaagcaag caaaatctac gctgcattag gcggagccga caatgtaact tcgatcgata
    18961 actgtactac acgccttcgt ttgcaagtca aagatacagg cctgatcgat caaaataaaa
    19021 tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg
    19081 taggtacaga agtacaattc gttgcagatg agatggcaaa actacatggt ggtgctgcag
    19141 cacgacctgc acaaacaaac accgtggtta aaacagaaac atttgccggt gaaacgatcg
    19201 aacaagatat ttacgctatc gcaaatggga aactgattcc aatcactgaa gtttcagatg
    19261 atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt attaccagaa aatggagaaa
    19321 tcttctctcc aatcgctggt acgatcacaa acattttccc aacgaaacat gctgtcggaa
    19381 tccaaacaga tgctggtatc gaagtcttgc ttcatatggg catcaacact gttgacttaa
    19441 aaggcgaacc attcacttta tatgtagaag aaggtcaaaa agtcgcaaga ggtcaattga
    19501 ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa aaacacggat atggtcgttg
    19561 tattcacaaa tggcgacaaa gtccaaagct tagaaatcga acccgcacgt gatgtaaaag
    19621 caaatgataa aatcggctct gtttccaaca aagcctgagc catcgtcttc cccagacttt
    19681 tttatcgata aacaaacagg agctgagaca aaaatcttgt ctcagctcct ttttccgaat
    19741 aaaaacggcg ttctcctgcc aactccgcgg taataagtcc agctaaacga aacatatgaa
    19801 gagctatttt ttatttgact cttcttatta cttgatgcag aacagctttt tcacaaaatt
    19861 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
    19921 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
    19981 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
    20041 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
    20101 tgcttcaata atttctaact ctttacctgt cgcacgctct aatgccttgg ctaattcttc
    20161 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
    20221 ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc
    20281 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
    20341 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
    20401 gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata
    20461 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
    20521 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
    20581 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
    20641 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
    20701 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
    20761 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
    20821 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
    20881 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
    20941 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
    21001 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
    21061 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
    21121 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
    21181 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
    21241 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
    21301 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
    21361 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
    21421 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
    21481 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
    21541 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
    21601 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
    21661 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
    21721 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
    21781 ttctgctaaa gcgcttacaa caagatacgt tccttttggg aatgggatga ttcgcgtagt
    21841 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
    21901 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
    21961 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
    22021 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
    22081 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
    22141 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
    22201 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
    22261 tgtaaaagag atctcgggag cttcgactaa cccataatct gaagaaggaa gccattctgc
    22321 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
    22381 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
    22441 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggt ctccatcaaa
    22501 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
    22561 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
    22621 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
    22681 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
    22741 ttgtatgccg tatctactga tttctgaagg cagatatcct gaccaatcac gaaaagctct
    22801 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
    22861 atctagaaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
    22921 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
    22981 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
    23041 gttcagatct ttaagcatat ttatcttcct ccttgattgc ctttttatca tattatcaaa
    23101 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
    23161 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
    23221 tatgttttac ctttagaaaa ataaattttg gggaagacag attctaagta aacaaagcgg
    23281 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
    23341 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
    23401 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
    23461 aaaaaacaaa aaaagttcca tctttaa
//
LOCUS       DACESX010000034        23440 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.034, whole genome shotgun sequence.
ACCESSION   DACESX010000034 DACESX010000000
VERSION     DACESX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.034"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            182..2017
                     /gene="tdc"
                     /locus_tag="IXF88_001137"
     CDS             182..2017
                     /gene="tdc"
                     /locus_tag="IXF88_001137"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1507877.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2322..3800
                     /locus_tag="IXF88_001138"
     CDS             2322..3800
                     /locus_tag="IXF88_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1507878.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3766..5097
                     /locus_tag="IXF88_001139"
     CDS             3766..5097
                     /locus_tag="IXF88_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1507879.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5211..7763
                     /locus_tag="IXF88_001140"
     CDS             5211..7763
                     /locus_tag="IXF88_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1507880.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYTLGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7770..9209)
                     /locus_tag="IXF88_001141"
     CDS             complement(7770..9209)
                     /locus_tag="IXF88_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1507881.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9555..9917
                     /locus_tag="IXF88_001142"
     CDS             9555..9917
                     /locus_tag="IXF88_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507882.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10103..10960)
                     /locus_tag="IXF88_001143"
     CDS             complement(10103..10960)
                     /locus_tag="IXF88_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1507883.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11080..11991
                     /locus_tag="IXF88_001144"
     CDS             11080..11991
                     /locus_tag="IXF88_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1507884.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11998..13644
                     /gene="mdcA"
                     /locus_tag="IXF88_001145"
     CDS             11998..13644
                     /gene="mdcA"
                     /locus_tag="IXF88_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ1507885.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13746..14045
                     /locus_tag="IXF88_001146"
     CDS             13746..14045
                     /locus_tag="IXF88_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ1507886.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14035..15711
                     /gene="mdcD"
                     /locus_tag="IXF88_001147"
     CDS             14035..15711
                     /gene="mdcD"
                     /locus_tag="IXF88_001147"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ1507887.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15715..16308
                     /locus_tag="IXF88_001148"
     CDS             15715..16308
                     /locus_tag="IXF88_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ1507888.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16332..17408
                     /locus_tag="IXF88_001149"
     CDS             16332..17408
                     /locus_tag="IXF88_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ1507889.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17433..18302
                     /gene="mdcB"
                     /locus_tag="IXF88_001150"
     CDS             17433..18302
                     /gene="mdcB"
                     /locus_tag="IXF88_001150"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ1507890.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18413..19177
                     /locus_tag="IXF88_001151"
     CDS             18413..19177
                     /locus_tag="IXF88_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1507891.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19666..21759
                     /locus_tag="IXF88_001152"
     CDS             19666..21759
                     /locus_tag="IXF88_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1507892.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21784..22086
                     /locus_tag="IXF88_001153"
     CDS             21784..22086
                     /locus_tag="IXF88_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1507893.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            22113..23402
                     /locus_tag="IXF88_001154"
     CDS             22113..23402
                     /locus_tag="IXF88_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1507894.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         7500 a         4028 c         5012 g         6900 t
ORIGIN      
        1 tcgctttttt gcgttgaatt atcagaaaaa tccgacttta tttatttttg tttgtaaaaa
       61 tctacgtgga tttgatgtta taaaaaaaaa attctcattt ttggcgctat agtgaagagg
      121 tgttacagat aaacacatgt atctgcaaga cattgaccaa caaaatatgg agggaagaaa
      181 aatgaaagat atggatatca aggccgtctt tataggcgac aaagctgaaa atggaccagt
      241 ttataaaatg ttgttgaaca agatggtaga tgaacatctt ggatggagag aaaattatat
      301 tccttcagat atgcctgcaa ttagcgaagg agataagctc actccagatt atctagccac
      361 ccgcgaccat atgatagagg tcttggatga agtcagtcag cgattacgcg caggatcgat
      421 cccatggcat tcagcaggcc gttactgggg acaaatgaat gctgaaacat tgatgcctgc
      481 tttattagct tacaactatg cgatgctgtg gaacccaaat aatgttgcat tggaatcttc
      541 tatggcaact tcgcaaatgg aagcagaagt aggacaggat tttgcttcat tattcaatat
      601 gacagacgga tggggacaca ttgcagcaga tggttctatt gctaatcttg aaggattatg
      661 gtacgcccgt tgcatcaaat cgattccttt agctgtcaag gaagtgctgc ctgaaaaagt
      721 gaaaaaaatg tctgagtggg aacttttgaa tttatctgtt gaagaaattt tggaaatgac
      781 cgaaagcttt acggacgaag aaatggatga ggtcaaagca gcttcttccc gtagcggaaa
      841 aaatatccaa agattaggga aatggctggt ccctcaaaca aaacactatt cttggatgaa
      901 ggcgttagat atttgcggag tcggcttaga tcagatggtt gctatcccag ttcaagagga
      961 ttatcgaatg gatattaatg ctttggaaaa aacaattcgt gaattagctg gacaaaaaat
     1021 cccaattcta ggtgtggttg ctgtcgtagg aacgacagaa gaaggtcaag tagacagtgt
     1081 agataaaatc gtccaattaa gagaaaggtt aaaagatgaa gggatctatt tctatttaca
     1141 tgttgatgcc gcatatggcg gctatgcgcg ttcactgttt ctaaacgaag caggagaatt
     1201 tgtgccgtat gcttccttag ctgaattctt tgaagaacat catgttttcc accactgcgt
     1261 aacgattgac aaagaggtat atgaagggtt cagagctatt tcagaagcgg attctgtcac
     1321 gatagatcct cataagatgg gctatgtccc ttatgctgct ggaggaatcg taatcaaaca
     1381 taaaaatatg agaaatatca tttcttattt cgcgccatat gtgtttgaaa aatcagtaaa
     1441 ggctccagat atgttaggag cttatatttt ggaaggatca aaagctggag caaccgccgc
     1501 cgcagtctgg acagcacatc gtgtattgcc attgaatgtg accggatatg gtcaattgat
     1561 tggggcttca atcgaggcag ctcaaagatt cagagaattt ctcgatcatc tgacttttac
     1621 tgttaaagga aaaacaatcg aagtctatcc attgaatcat cctgatttta atatggtcaa
     1681 ttgggtattc aaagaacaag gctgtacaga tttgaacgct atcaatgaat taaatgaaaa
     1741 aatgttcgat cggtcttctt atatggatgg cgatgtttat ggtgaacggt ttattacttc
     1801 tcatactaca tttacacaag aagattacgg tgattctccg attcgttttg ttgaaagaat
     1861 gggtctaacg aaagaagaat ggaaaaaaga acagaaaatc actctgctac gtgcggctat
     1921 catgactcct tatttgaatg atgatcgaat tttcaatttt tatacaaaaa aaatcgccaa
     1981 agcaatggaa aagaaactaa acgaaatcat ccaatagaac tgatttttgt gtgaaagaat
     2041 aacaagagac atatggagaa cagacaatta tttgcttcat caaaacaatt gtatttcccc
     2101 ctgtttttca tatattcctc gccaagatat tcttttttat aaaacgttct ataagttgga
     2161 tcgttcgttt catgtatcaa acccagttca ctaagatcgt tcaatggatg tttcggtgat
     2221 actgggtttt gtactgaatc gatgaaacct tgtcatttct gttcttgaac aaaaataaaa
     2281 gaaaacaaaa acatatgtcg ctattgaaag aaggaataaa gatgaaagat actggttcat
     2341 caaagcaaat cacgaccatg acctttatcg gaatgacctg cgcccttgta gcaagtgtac
     2401 gaaatattcc agatgtagca gcaacgagct ggacgatgat tttttacatg ctggttgcag
     2461 tattattgta cgcttttcca atctcattga tctctggtga atttgctgga atgtttcccc
     2521 aaaaaggtgg tccagaactt tgggtatcga atgctctagg aaaaaaatgg gggttcgttg
     2581 tctcatggct gttatgggtc caaatgtttc ctgggatggt gatggtcgct tcagcactcg
     2641 ctccgttgtt tggtaatatt atcgataatg tgccgcttgg tttgaacagc aaatttacat
     2701 tagttgtcat tttagtcgtt tattggatca tcactttttt gaatttgaaa tttgatatgg
     2761 caaaaatcgg cggaaaagtc ggtgtttggt taggattgta cgtgcctttg acgatgatgc
     2821 tcttattagg gtttgctgcg tggatcaaag ttgggatcgt accaaccgga acattaggca
     2881 gtttttcatg ggacaaatta ataccagata ctacgactgc atcctcattt gtttattttg
     2941 cgccgatcat gtttattttt actggtatcg aaatgtcctc tgtgtatatc actcgattgg
     3001 aaaatccagt taaaacgtat attcgcggag tatttgccgc gttaattttt atatttttcg
     3061 tcaatatttt gaatgcttta gttgtagcaa atgtcgtacc aaaaggacaa atggaattga
     3121 ataatatcgc gcaatctatt tcgatttatt gccaaattct tggtttgcca catcttattg
     3181 tgaatctgtt tagtttactt gtcttcattg gtgtagcagt ccaattatct gcttgggcct
     3241 caggaccagc aaagacagtg acagaaagtg ctagaaaagg tgcgtatcca ccaaaattca
     3301 atttttggaa gacgaatcaa tttgacgtat ccaaatccgt tattttgact cagtctgtca
     3361 ttatctcaat ctttgcactt ttttatttgc tgattcctgg cgtcaatcaa gcatttttga
     3421 tgctcgtaaa ttcaactact gttatctact gtatcgtcta tgtcatcatg gggatcgcag
     3481 ttttgagatt gcgttataca catgcaaagt tgaatcgacc atttagaatt ggaaaaaaag
     3541 agaaaaagag caatcttgga gtttggactg tcgttattgt tttatttgcg gcaatcggat
     3601 tttctgtcgg actgacgatg aaagctggaa catggatcaa tctaattgca gtcacagcca
     3661 tcagtgtcat tttgtttgta gtaccactct gtattgaaaa aataaagaaa ccttcttggg
     3721 aacaggaagt cgccatgctg cttgaaaaag aaaaaggtga acttgatgga aaaaccggca
     3781 atgaaacgga ctctaactaa tcgaaatatc cagttaatcg cattaggtgg cgcaattgga
     3841 acaggattat ttttaggttc agcaaaggca attgcatcag cagggccagc tgttattctc
     3901 gtttatttga tatgcggttt ggctatttat ggtttgatgc gggccatggg tgatttattt
     3961 ttagcaaata gtacatttaa ctctatcagt gatttcgttg actattattt aggcaaaaag
     4021 tgggcatttt ttgtcaattg gacttattgg ctttgctggc ttggagctgg acttgcggaa
     4081 ttaacagcaa taggattgta tatccatttc tggtttgtac atgtaccagc tttgataagc
     4141 ggaggtagcg cactaatgat cttaatggca gtcaaccttg ttgcagtcaa gtggtttggt
     4201 gaattggaat cttttttctc aatcatcaag atcttgggga ttttcctttt tatcttattg
     4261 ggtataggat tgagtgtctc agattttcat acccagtcta tcggaatcaa agaaaatttt
     4321 ggacagatcg atgaattact accgtttggc tttgctggaa tggtagtctc atttcctatg
     4381 gttttattca gttttgctgg aattgaaatg atcggcttaa ccgttggcga gacaagtgat
     4441 ccgcaaaaga atttgaaaaa agcaatcaac tgtctgcctt ggcgtattct ctttttctat
     4501 ataggaacga ttttgtcgat tttatttgtt gttccatggc attgtttaga tttaaaagaa
     4561 agtcctttag tgactatgct gcaagtgatc cattgccaag gcggagcagc aattgtcaat
     4621 ctaatgattt tggttgcttc tttgtcctca gcaaatagtg cagtctacag tactagcagg
     4681 attttatatc aaacagcaaa agaaaaaagt ttgccccatt ggttttgtgc gctttctaag
     4741 aaaggcgtac ctttacacgg tattttaatt acaggaatgt tattttttgc cggtctttgt
     4801 ctacattttt ttgtagctga tagtcgattg ttttttcagc ttttggcagg catgacaacg
     4861 agttgttttc tatttgtgtg gtcttcgatc ttgtgtgcac atgttcaatt tattaaagaa
     4921 aagaaacgga acactacaca aagatataaa gatcttgctt tgttggtgtt ttttacttca
     4981 attgctttca gcttgttatt cgaaagcacg acacgcttta ttcctttttt attggttatc
     5041 tggtttgtat tcttaaattt tgtatataaa agaataagca atagaaatat cagttaggcc
     5101 cagatacatt ttgcaaaacg aattatttca gacccccctg aaataatcga tacgcctgaa
     5161 aaaacttccc tgcgtataat aacagtatca attaaacagg aggggatagg atgaactggt
     5221 acagattgac ggtagaagat gtattgaaaa actatcaaac atcaacagaa ggcttgtcgc
     5281 aagaagaacg acagaaacgc ctgactgatc aaggatataa tgaaataaaa gcaaggcagc
     5341 agacaaaacg atggaaaaaa atagcaaagc attttactga tctgctgatg atcgtgttga
     5401 tcattgcctc ttttttgaaa tttgcttcta gtgaatatat tgaaggaagt attattttat
     5461 tcgtagtgat cgtcaatgga ctcgttagtt actggcaaga aagaaaagcg gaagaatcat
     5521 tgaatggttt gaagcagctg atgggacaag aagcgatagt actatcaaac gggatgcaag
     5581 aaaaaatccc tgcgaaagaa ctggtaaaag gagatgtcat tgtcttacgt gccggagatg
     5641 tcgttccagc agatatccga ttaatcgaag cacatgattt attagtggaa gaatcgattt
     5701 tgacgggaga atcagaagca tctgaaaaaa gtcatgccgt tttgacagaa gaagaatcga
     5761 tcggcgacca gaaaaatatg ggtttttcag gaacgcttgt acaatctggt tctgcagtcg
     5821 gtgtcgttgt agaaacagga aacacgacag aaatcggtaa aatcaatcaa gcattgcaat
     5881 cggtcgaaca acaaacgacg cctctgatta aaaaaatcaa ccgattaaac aaacaaattt
     5941 ttcaaggcat tctttgtttg agcctattct tagttatttt tacgacattt cgatatggta
     6001 tggattggca tatccttctt tcggctacga ttgctttggt ggtgtcaatg gtacccgaag
     6061 gattgccagc tgttttgacc atgattctat ccgttggagt acatgaaatg gcaaaagaaa
     6121 aggcgatcat caaaggactt ccttcggtag aaacattagg ttctatgaca gtgatctgtt
     6181 cagataagac aggaacattg acaaaaaatg aaatgacagt tgtggatgta gctgcaaaag
     6241 aagaagcctg cgtgttgtcc attatgaaaa actgtcagga attaaaaaca aaagaagaac
     6301 agaaaacaga aaatttaagt ggcaatccga cagaagttgc gctgattcga cacacagaaa
     6361 cagccaatct acctttgaaa aaaatagaag caaagattcc gttcagttct gaatacaaat
     6421 atatggcaac gatgcatgcg gaggaaaaag gagcggtgat ttacgtcaaa ggtgcaccag
     6481 aagtattatt tgctaaatca actctttctt ctgcagaaca agaagcatgg agtcagacag
     6541 ctgcagaatt tgcgagaaaa ggtcagcgtg ttttaggatt tgcttataaa aaagtagatt
     6601 cgaaacagga actcacgcat gagactttaa cccaattaac ttttgcaggg atcgccggat
     6661 tgatcgatcc gccaaaagaa agtgcagtca aagctgtaaa ggaatgccaa caagcaggga
     6721 tctcagtgaa aatgattaca ggggatcaca aagacacagc aaaagcgatt gcggaacaaa
     6781 ttggcttaaa acatactgcc aaagttttag aagggatcga tttggatctt atgtctgatg
     6841 aagagttgat tcagcaagtg ccaatagtcg atgtctttgc tcgaacgaca ccagaacata
     6901 aactgcgaat tgttaaagct ttgcagaaaa atggagaaat cgtcggaatg accggagacg
     6961 gcgtaaatga tgcacctgct ttgaaaagat ccgatgtagg aattgctatg ggaatcaaag
     7021 gtagtgaagt aagcaaacag gcagctgata tggttttagg agatgataat ttccatacga
     7081 ttgcaaaagc cgtaaaagag ggccggcgga ttttggataa tttgcaaaaa accatcaatt
     7141 tcttcttgcc aactgcatta gctcaaggat tgatcttgat ttgggcactt atgctgaatc
     7201 gcccgctacc tttgtcccca gtacagattt tatgggtcaa tatggtaacg acaatcactt
     7261 tatcttatac actagggttc gagtcgccag atccagaaat catgaagcgc ccaccaagag
     7321 atccaaagca aggaatccta tctggttatc atttgtttcg gatcatctat gtctccctac
     7381 tgatcatggt tcctgcctat ttattggcta tgcagtttga agggcaaagc ttacagcaaa
     7441 ccattcttct gcaaaatatc gtcttagctc aagctgttta catgatcaat tgcagagagt
     7501 tgtccaagcc gtcattgaac caaggtcttt tgcaaaacaa agcgcttttt ctttcattag
     7561 gtatccttgc tttattgcaa gggatggtta tttttctacc agtcggccag cagctgatcg
     7621 gtacagtaag tttaacgatg acacaacagt tattgattgg agcaaatgtg attcttctat
     7681 tcttagttgt agaaatagaa aaagggatca tgaacaaact gtttcgcaaa aaagaaaaaa
     7741 cagctagaga atacagtcgc tagccgttac tataaaaaag ttttgaaagt gagttcagtc
     7801 aaacgattga gctcattttt cgttggaata gaagaaatgt atacaatagg gatatctaga
     7861 gaatctagag ggaaattgga aatcaagata tctgtttttt caaaagaaat agtggaaaga
     7921 tcggttagtt cgataggtaa gaagcagaca cgttttccta agtaatcttt taattcctgc
     7981 tgcaagaacg ccttccatga aggttctcct tggaaaataa aatagacatt gaacaaattc
     8041 ggtttgatag aaaaaatcgt ttgttggatc agtagagaca agagttggag aaagtaattt
     8101 ggttcattga tttctatttc tttttttaga tcgttcagta aattctcttt ctgttgatac
     8161 aagtgtgcat attgctgatc atatgtttct aaaaaacgat ggtattgacg gtccaatgct
     8221 tcaatcaata tcggcgattc atgatatttt aatatgagga gagccaagtt atcagtcaga
     8281 gatgtttttt ttagttgatc aagagacagc tctgaagcta gttcctctac aaacgtttcg
     8341 aggacctcta aaaatggaga atgatgcagg cagcgagcgg tttcttgtcc atagttattg
     8401 ttgtaattcc aactttccag aaagcagaaa gaagcaaaag aaagctcagt gtctttaaga
     8461 taaatacctt ccgttaaata caattttttc aatgcagagt catacaggga ggagagtgtg
     8521 ctgttggtat ggatagaaga atctaatcgg caattcgctt ttatacggat cgtattgata
     8581 aagaaccaac atgttccgaa aatctcttct gtttcaaccg ctagttcagt ttgactgagg
     8641 tttcttaaaa attgaaaata atgggattca tggatcgaat aatcctcgaa aaaatagttg
     8701 ttatgagtga aaggaatcag caatcgatga taaaaagtac gaatcgcacg ctcttttcca
     8761 agaatacaag ctgtatattt cgtcaaatcg atctgcaagt cgtaacttgc taaacgctga
     8821 ttgattttag tcacatgtct tttcaatgtc ccataagaaa gcccatggtc ggctagaaaa
     8881 tcagggacag caactttttt tgtaaaaagc agatttttca gcagttcgac actgactgat
     8941 tgtttgaaaa aaatctccca aaggtcattt gttaataaat tttgctggtt ttgcaaactg
     9001 attcctgctt gatcgctgat gatgtcccag ccttcgggca gctgagagcg aatagattga
     9061 aggtcagaaa aaatcgttcg ttcggttgtc cctatgattt cagctaattc atttacggtt
     9121 atctttgtat gattcagcaa ttgttctact aaagaaacat gacgtcgaat atctttttcg
     9181 gtaatgattt ttttcattag tgaatacatc aatcagactc ccttctattc taagaatacg
     9241 gagaaaataa acggtggtca agtttcaact gatttttcaa tcattgttta tttttctgga
     9301 gcatttactg atgataagaa tttcatcatg aacttgaaaa atgataccac taaaatcaaa
     9361 aaataggtgg gcaaacaata ataaacattt taatagtaga tggtagcaag accaacacag
     9421 gcggattgaa catttaagag ttgataaaaa agaaaaatat cgaagtattt gattagcaac
     9481 tacttgtatt catttttcta ttcttacgtg gtatctttga ataaagcgaa tcaataagaa
     9541 agagatggaa gcagatgcta acgaaaaaag aactaatcga tcacatcaat gaaacataca
     9601 atttgtctcc agaatatatt tttaaaaaat ttcctgatta ttgcgtattc agacatcaaa
     9661 aaagcaaaaa atggtttggg gtcctgatgt ctgttccaga agaaaaaatc tatgatgatg
     9721 caagtgacca taggatcatc aatattcttg atttgaaggt cgatcctgaa cttggcggaa
     9781 ttttgcgagg aaaagagggg tattttccag cctatcacat gaataaagaa cattggatct
     9841 ctattgatct acgtaaaata gaaaagctag atgaactagc agatttgatt gatggaagtt
     9901 ttaaatcaac gcaatgaatg tctaggacaa tagagaagag gttgtgacag aaatgtttag
     9961 cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttggg aaatttcggc
    10021 ttattttcta agaaattact tctggaacac cgtttgttcg aaaaaagagc tgcgacaaga
    10081 attttgtccc agctcttttt ttttattttc ctaaataaaa ttcccgcatc gtttttgcaa
    10141 tcattttgat caaaggatga tcttctgtga agctagaaga taaataaatt gttcgtgtca
    10201 agggcaaatc tttgatttcc cgcaagacga aaggatcatt tttatcgtaa gaccaagaaa
    10261 cttttgggaa aaagctaaca gctaatcctt gcttgacaat agaacgtaat gtcgcaggat
    10321 catctgtcgt cgaactgatc gtcaattcga tacttttcgc ttcaagtgct gcatccagtg
    10381 tatgacggaa ggcgttgctc tttgacagca tcaagatggg ggtgcgttgg atatcttcta
    10441 attgcagatg ttctttttta gcaagaggat gtttcttagg aatggcgaca taaacaggtt
    10501 ctttcatgat aggaatattg gtcatgccag gcttcatctc cgaagtgatg tacagtacag
    10561 gatcggtaat atcatctggt aaatgatgtt gcatgatcgt taattggata tcaggatgct
    10621 tttccataaa aattcggacc aagttcggaa tagaaacaga ggctacctct aaatacaata
    10681 tgacggaagt aacgatcttc tcattgaccg aagacaataa tttgattcct tcttctaatg
    10741 tgtctaatgt cttgactgca tatggataaa attcttctgc ataaacggtc gtctcaatgt
    10801 ttctgccgct atggtaaaac aaagggacac ctaattcgtt ttccaaatgt ttgatcgcac
    10861 ggctcaaggc aggttgagaa ataaataatt ctttggctgc ttgggtaaca ttttttgttt
    10921 cgaatacttt tttaaaatac tgcagatgaa gcaatttcat aattatccat cccatccata
    10981 cctttttgtt atggtttttt ctttttttgg cattttttat tatagcagaa attgactaag
    11041 atagcagtgg aaaaagtaag tgctgaggag gcgaggctca tgagtacagg tttcttattt
    11101 cctggacaag gcagtcagaa gttagggatg ttagcggata tccctgaaat gtacatggaa
    11161 cgtttagaaa atgtgacggg gtatcggtta gttgataaag aagaaaacta tcaagatacg
    11221 gtttttatcc agctagcatt actgacaaaa gcagtatttt atttagatga aatggaaaaa
    11281 aacggtattc agcctgatat tgcagcagga cattcgattg gtgctttttg tgcagctgtt
    11341 gcttgtcgat ctttgagatt tgagaatgca gcggaactag tctatcaccg cgcagtctta
    11401 atgaaagaag cttatccaag cggttatgca atgggcgtag tagtaggtgt gacgagaagc
    11461 gaagctgagg aaattgtagc aaaaacattt gatcctgacc atccagttta tctgtcaaat
    11521 gaaaattgtc cgatgcaaca tacgttatct gggagtatcg aaggtttatc cgttacgttg
    11581 gcagaagcca aacggcacca agcgcaaaca gcgaagctgc tgaaagtccc tgtgccatct
    11641 cactgtctct tgatgaatga tacggtcaag aaattcaagc cgtatatgca gtctgtccat
    11701 ctatctgatg ctagatgttt gtatataaaa aatacggatg gaagagccac tcaggacgca
    11761 gaagagattc gtaaagattt gttggaaaac ctagctttcc ctgtgcagtg gaaccaaatg
    11821 atggacttgg caaaagaatt aggaatggat ctgacgattg aatttccccc cggcaatact
    11881 ttgacaaagc tgattcatac aaaatttggt gtagataaag ggatccgtac gatcaatcta
    11941 gaccaacatg gtattgatga cgccacgttt ctatatcaaa aatggagatg actaattatg
    12001 gaaaaaccaa accgcaactg ggcttctaaa cgcgaaaaaa aagcgcagcg attggaaaaa
    12061 atcagcaact tgctcaatgg caagtatgta gaaactgaaa aaatcgtaga agtaatggaa
    12121 aaactgattg ctcccggcga caaagttgta ttagaaggcg acaatcaaaa acaagcaagt
    12181 tttctttcag aaagtcttga aaaagtagat gcagaaaaag tgaatgatct gcatttgatc
    12241 atgtctagta tctcacgacc agaacatttg aatatttttg aaaaaggaat cgcaagcaag
    12301 atcgatttct cttatgctgg tgcacaaagc ttgcgtgtcg cccaaatgat tgaagacggc
    12361 acaatgaaat taggtgatat ccatacttat ttggaacttt atggacgctt attcatcgat
    12421 ttgattccaa atgttgtttt agtagctgca gataaagcag ataaagacgg caacttgtac
    12481 acaggtttca atacggaaga aacaccaaca atcatcgaag ctgcagcttt caaagatggt
    12541 atcgtgatcg ttcaagtcaa cgaagttgtc gattctttac cacgtgtaga tatccctggt
    12601 gactgggtag acgttgtcgt gaaagcagat aaaccttatc aattagaagc tttattcaca
    12661 cgagatcctc aaaacatcac agaattgcaa attctaatgg caatgatggc aatcaaagga
    12721 atctacgcag aacacggcgt acaatctttg aatcacggta ttggattcaa tacagcagct
    12781 atcgaattgt tactgccaac ttatggtgaa tcactagggt taaaagggaa aatcgcgaag
    12841 aactgggtat tgaacccaca tccaacgatg attccagcca tcgaatcagg ttgggttgaa
    12901 agcatccata gttttggtgg cgaagttggt atggaaaaat atattgctgc tcgcccagat
    12961 gtcttttttg tcggtaaaga cggcacgatg cgcagtaacc gaacattatc acaagctgca
    13021 ggacaatatg ccatcgatat gtttatcggt tcaaccttgc aattagatta cgaaggaaac
    13081 tcttctaccg taacaaaagg acgtttatct ggttttggcg gtgcgcctaa catgggacat
    13141 aatcctggcg gtcgtcgtca ttctacacca gcatggcaat cacttcgcga caatgatgat
    13201 ccattaggga aaggccaaaa attagttgtt caaatggttg aaacatatgg ttcaaataaa
    13261 aaaccagtct ttgtcgaaga attagatgca ctagctgttc aaaaacaagc aggcttagcc
    13321 aatgcaccag tgatgattta tagcgaagat actacacata tcgtgacaga agaaggaatt
    13381 gcttatttgt acaaagcaaa aacaaaagaa gaacgacaag cagcaattgc agcaattggc
    13441 ggtgttacac ctcttggaat gactagcacg aaagaatcac tagatagctt gcgtgcagcc
    13501 ggcatcgtga aattaccaga agatctaggc atcaaacgta gtgaagcgaa acgttcatta
    13561 ttagctgcac aaaccatcga tgatttgatc gaatggtcag atggcctgta tgaaccacca
    13621 atgaaattca gaacttggtc ataaaattgg cgagaaaaac cttttgtcgc catttaccca
    13681 gatggaagaa gaaagacgaa taagcagaac gtgtatgctc tgttttacaa aaaggggaga
    13741 gatccatgga acaattagct tataaatatc caacgaaaca gccgatcaat aagaaaatcc
    13801 atgtaggagt ggtcggttca ggagatttgg aaatcttgat gtttccaaca gaaaaaacag
    13861 aatctactgt aaaaatttgt acaggaagcg atggcttcgg cgaagtatgg aacaatgtat
    13921 taacgagatt ttttgatcgt tatccaattt cagcagatat cgtgatcaat gacttcggtg
    13981 ctacaccagg agttgtgtac ctacgattga cgcaagcaat ggaggaactt tcagatgaaa
    14041 aatagttttg tagaattaaa cgccagagaa cgagtgaacg ctctacttga caatggacgt
    14101 gaattgattg atccattcga tcaaatcatc gcgccaagtt taccagcaca aggaatcgtc
    14161 cctgaaagtg atgacgggat cgttgtcagc cgtggcacga tcaatggcaa acaagcagta
    14221 gtaatcgcta tggaaggaaa attccaaggt ggcggtatcg gtgaagtttc cggtgcaaaa
    14281 atcgttgctg cacttgaaca tgtgttagaa gaaaataaaa aaggaaatga gatctttcca
    14341 gtcattgtac tagatacagg tggtgttcgt ttgcaagaag caaactacgg actgttatct
    14401 atttcagaaa tcggaaatgt aatcattgct ctaaaacaat atgtaccagt catcggtttg
    14461 attccaggac gtgtaggatc atttggcggt atgtcgatta cttcagcatt aatgagttat
    14521 ttaatcgcaa caaaaaaagc gagagtcggt ttgaatggac cagaagttat tgaacaagaa
    14581 gcaggcgttc gagaatttga ttctagtgac aaagacttga tttggaatac attaggatct
    14641 cgtcaacggg tagaatcaaa aatcattgat gaattagtga ctgatagtgt agaagcaatc
    14701 aaagctgctg tcatcgcagc aatggacgaa cgaaaagaca cgcatcgcag tgaaagctca
    14761 gcattttatt tatcattact tgattcttta gatctttcaa aaccaatggc cattgacgat
    14821 tacaatgaag caattgctaa ccatcaagca aaatcaattg acttaccaga agtagcagaa
    14881 ggaaatgaac cagctgcagc aagtgttggt tacgattggt ttgcggcact aactggcatg
    14941 aaaaatccaa cttcttctat tagtactgtg tattatggta aatcaagtaa attcatagaa
    15001 gaagccatcg tgacatcaat cgtacctgat cctaaaaacc cgatgtaccg tgtacgaaac
    15061 ggtgaagttg gtttagttga aggtttccgc atgggacaaa ttttggatca cgtttacgaa
    15121 gaagataaag acaaaaaagt gaaacggcca atcatcgttg tgatcgatgt accaagccaa
    15181 gcatacggct ataacgaaga attgatcggt atccatgtgg ctttggctaa tagtgctgct
    15241 gcttatgcaa aactccgtca agctggacat ccagttattg gattgattgc aggaaatgct
    15301 atttcaggtg catttttagc acatggatta caatcaagcc gcttgattgc tttaaacaat
    15361 aaagaaatta ccgtccaagc gatgtctaaa gaaagtgctg cacgtgtgac aaaacgttcg
    15421 gttgctgaaa tcgaagcagc tgctgaaaaa gttccagcta tcgcttacga tatcaataat
    15481 tatacaaaac taggagcgat ctatcatttc ctagaagata tcaccgacca agctgcttca
    15541 gaacaaaatg tcagcaaagt catcgaagca atcaatgaag ccattcaaga tgtccgcaat
    15601 acaaatgaca cgatgctgac aaatcgatac acaaacgaaa cagccaaaac attcggacgt
    15661 gtcgaaacaa ataaagtccg tgccaaaatg gacgaagaat gggatgcata aaaaatgatt
    15721 caagcacatg atattgtcct ttttgataaa catgaaatag aagggttcac cttgcctgaa
    15781 tgggtcgttc taactgaaaa gatgttcgga acagttcgtc gtgcgtcgac ggatcacgca
    15841 gaaatactac cgattggttt acgtggacct gcccgcaatc agagatggtc aacagaaatc
    15901 tcaaaacatg ccgtcaaaca agtcattcat ccgtgggaca tcatcgaaca agaatcgtat
    15961 agacaagaga aaatcatgga ttatcctgtt tatcagcaat ttcatgctgc tagaaacata
    16021 ttatcaggat gtaaatgggg aatcggcggc agcttaggat ttgaattatc gactgggatt
    16081 ccggcagtaa aagaaaccag tgattttgat ttgctgctgt acgcaaattc accgattgaa
    16141 ttgccaattc aggtaattca atcgcatcct gctttttttg agcaattcga tacccaagta
    16201 gtgactcggc aaggcggttt ttcactaaaa gagtatttga gagagccaga gaaaaaaatt
    16261 ctattgaaaa cagtcgaagg acctaaatta accaaagaac tttggtaata aagaaaaaga
    16321 gagagaaaga gatgtttgaa aacaaaaaaa tcaaaatcaa cgtaagtgac ttccagaaag
    16381 tatcagctgt aacggctgtt atgatgcaaa cgatattgag ctttgctttg gcaaacagcc
    16441 acagcgcaac aaatacagct tttcttggta ccttttacgt catcgttaaa tacacagcac
    16501 caatgtttat ttttgcaatt gtttataata tggtaaaaac aagtcagcat ttgtcttatt
    16561 gggaattttt gaaagaaaag ttttttgaac tagtcgttcc gtatcttcta tggacgacgg
    16621 cttatctttg gctatttcca gcagtccaac aacaaactcc gtatacggat actgcaagct
    16681 ttttactgaa atatgtcaca ggtgatggag cgcctcattt atggtatacc gttatgatgc
    16741 tgcagatcca attgttcatg ccgttttttg tttggctcgg ttacaaagta tttgctaata
    16801 aaaaatatgt atggccagta ttgattggtt caacgatttt atatgtcgca tggtatctgt
    16861 tctatcaaac acaagtattg actgggcctt atcatgacag ctggtactta ttagatcggt
    16921 ttgttgctag tttcatgatt tatggggtag ccgcatttgt ttatcacgaa aaagtctatc
    16981 aatatttgga cagagtacgt tatcttttct tacctgttgc cttagttatc gcgttctttt
    17041 ctgtccgttc attgctcgca catccaggag acttgagctt tgcgaatgca ccttacttga
    17101 atacgatcca aagtttatac agtttagtca tcatctttgc tgtcttcata ggcgcttcca
    17161 aaatgattgt aaacgattcg ccaaaattgc cattgttcaa atggttgtct gtttatgctt
    17221 atcgtacgta tttagccaat gtatttgttt tccaagtact gttattattg tttaaagata
    17281 gttggctgca gttaccttca ggagtcatga ttcttgtagc ttatctaatg acggcaagtt
    17341 gtgcatttgc tttaagctgg ttacttcata tcatttgggt agctatcaaa aaaggtttca
    17401 gcaagtaata gtaggaagaa ggagtgagtg tcatgataaa tgacgaacga aaaaaagcag
    17461 tgactcagca actcgctgcg ttggtagagg aagcattgat cgcagaagta acattaagtc
    17521 caaaacctgg attagtggat gcactggatg cgggtgcaca ttccgatatg gactatgctt
    17581 tatttcttaa gtctgctaag acgctaactc ctttttttga agaaatggct caaatagcat
    17641 ggcatcatgc tattgatcag gagttaagag aaagaattgc tgagattggg cgaaaagcgg
    17701 aaaaggcaat gctgattgct acaaatggca tcaataccca taaaggagcg atttgggcaa
    17761 tggggctatt gatcagtgtc attgcacaaa aaatcagtat ggaacagcct ttttctttat
    17821 cgaaaatact tgatgatgtc agcaagcttg ctgcttttcc tgatacaaaa tatcgtcata
    17881 aaaaaaccac tcatggtcat agggtgaagc aaaaatatgg cgtaaatggt gcatatgaag
    17941 aagcgatatt agggtatccg catatcaggc ttgcaattca agcagctaag gagctagata
    18001 atgattcata tgtacagaaa caaatgcata tgttgttagt tttgatgagt tcgctagatg
    18061 acacatgcgt attgcatcgc agtgatcaga aaacgttagt ggaaatgcaa aaacttgctc
    18121 atgctgctag tcaaagtgct ttaccaaatc gtccatttac agcactgttg cacttttgtc
    18181 aggaaaacca aatctctcct ggaggaagcg cagatctctt gtctgcaagc ttgttcatgc
    18241 tgaagataga ggaattatgg actgccgaca catacatttg tccgatgtta ctcaatgact
    18301 aaataagaca aaattgctaa aaaaacagtt tatgcatctg gttattgact cttcctttta
    18361 gcaaggctat cataaaaata gttttttgta ataaaagata acgaggaaga caatgaaaaa
    18421 aatcaattat ttagcaatca ttttgttagt gggcctatgt ttgtttgctc tgccgaccca
    18481 aggattcgct gtcgcgggtg gtcatggtgg aggtacatct agcggtcgaa gttcgggcag
    18541 aggttcattt ggtggtcgtt catatggggg cagttatttt ggcagtcgtt cttacggcag
    18601 agattcttat gggtattatg gtccaggtac aggtccttct agttatgagc aaacgggatt
    18661 tcttgcatta gctgttttgt cagtaggagg ggtttctgct ttttctatcg tcaagaataa
    18721 aagacaaaag aaaaggctga aagcggagat attgtcccgc atgtcaggaa cggctaaaga
    18781 aaagaaagca cagtttctgg aaatccaaaa gatattcatg aagatccaat ccgcttggga
    18841 agaagaaact cctgaaaaag ccagaagctg ttatacagac aagctgtttg ccgaacatca
    18901 gaaagttctg caaaaaaaca aagcagacgg tgtaagaaat catactaaaa aaataactat
    18961 tcaggaagta ggaaattatc gacagatcca cgaaaatagt tttgccatcc gaattgattt
    19021 cagatgctgt gattattcag tcgatcgaaa agatgggcga ttgatctcag gaagtaagac
    19081 acaacgacag tatttttctc agatatggta ttttgatttt tctgagaaag aaaaaagatg
    19141 gcaagccgat tttattcagc cgattatgct ggattgacag gaaatattta tgaacggagt
    19201 tgtgacaaaa gccttgtcgc agctcctttt tctgaataaa cggtgtgcca gagatagcct
    19261 atcggcaata aggcacgatt ttgtaaaata tgggaagcga tttttcaaaa tcgcttctta
    19321 tcgctcaagg ctgaacatct ctgtcacaac ctctccacta aatggtgttt aaaagcttgc
    19381 atctgcgata ataagaacaa ccaaacgaaa aataggaagc actattttag tttagttgtt
    19441 cttattactt gatgcaggac agctttttca caacctcttt tttgtataga tgggcaaaat
    19501 ttttgtaata ataggcgtat gtctttttcg ttgaggagat gaaaaagcat atggcttatt
    19561 ttttgtttgc tttgcttact ttctttccac ttcaagtggc agaaatatct actttttatt
    19621 atgtaagcgg aaccatatac ttgaaatcag aaggagatca tttctatgaa aacaagaaca
    19681 attgaaatac ttcacagaat catgacagcc caatatccag taaaagcaaa acatctagca
    19741 agtgagttca atgtcagtca gcggacgatc cgtcaggaag ttcttgaagc gaatatctgg
    19801 ttgagcagta aaaagcttcc ggagatccgt acaattcgca acaaaggttt tttgttgaag
    19861 ctggaaaagg aaaagcaagc tcgattagaa actgaaataa aagatgtgaa agacggcttt
    19921 ttgaatcggc gcgaacgtat ttttgatcta gttttagccg ttgcttacag ccgaatcccg
    19981 atccatctaa acaaaaaaga agaagaattt caaatctcta aaagtacatt agatgaagat
    20041 atgagacggt tacgagcaac actggtcagt tatgggatag agatcgtcag ttatggaaag
    20101 caaggactgg tttataaagg ttcagaacga tctatccgta cgatgatcta tgattttatc
    20161 aatcagaatc tgggaatgac cgacttcttt atgaatcaag atgcacaatt aacaccggct
    20221 caaaaaatct ttctccatta ttttcctttg gtggaaatca aaaaaataca ggagatctat
    20281 tccaataagt tgttgaaaca agaagatgat atttataaaa aacaaatcct gctttttaca
    20341 ttgatctgga tccagcgaat caggaaacat gaatttatct ctgccattaa ttggaaaaat
    20401 acagaaataa aagacagtag cttttctgca tttattgacc acatgatcga ggaatttcaa
    20461 ttggacaatg tcccgcaagt agaaagaaat tatcttcgtt tcactattga gacgttcaat
    20521 gctagagata tctccaattc aatcgagtgg gtacaagctc aattactaac gatccaattg
    20581 atccaatttg tagagaacga gacacacgta cctttccatc taaaggaaga agctttattc
    20641 gaaaatctct ataagcacat ggctgcactg attgtaagaa tcaagaatca tatccaagtc
    20701 atgaatccat tgaaagaaaa catccgaaca aattatttcc caatctaccg cgcagtcact
    20761 cattttgttc ctacgatcga agaagtaatt ggtggcaaga tactggagga cgagatagct
    20821 tttcttgtca tccacttttc gacgatcgcc agtgtaatca agcgagactt gaattatgtt
    20881 tataaatcag tcgttgtctg taaccatggg atggctactg gaaatctgtt agcagagaat
    20941 ctgaaagaaa agtttcctca gatcgagatt actgcagttt taagttcaaa agaaatcgaa
    21001 ttagtagata aattggatgt caatctggtt ttcagcacct ttcaattgaa ttatcgaaat
    21061 aagccgcttt tagttgtcga tccgatttta actgagacga atatcttggt tatagaagac
    21121 tttttgaaaa gaaatgaaga actacagcgg ttagagccta acagtcatga atcgacggag
    21181 ttattcactc aaataataga tgtcatgaaa gaaagtggtg gccgagtaga tcgaatcatc
    21241 tatggcaagt tagaaaaatt attcgaaaca aacaatttag aaatcaacaa aagggagatc
    21301 cagcctatgt taaaagatat tttgacggac agcaatatca tgatcaaaga aaaagccact
    21361 acttgggagg aatcgattga actagctgct agaccattgg ttaaagaaaa cattgtcgaa
    21421 gagcgctatg tcgaagcgat gattcgtgca gtgaatgatt acggaccgta tatcgtgatt
    21481 ggcaaacaca tggcactagc ccatgcccga ccagaagatg gggtcaataa attaggagtc
    21541 agcgtagcta cgattgagca accaatcgat ttcggtaatc ctgaaatgga tccagtaaag
    21601 attatttttt gtttggcagc agtagactcc tactcgcatc tgaacatcat gaaagaacta
    21661 attgaattga tcaacaacga agagaagttg gatcgattga ttgaatgcac aaatatcgaa
    21721 gatttcaaac aactgctttt ttcagtagtt gagaaataat aaatgatacg ggggagaaca
    21781 aagatgaaca aattgaacat tttattcgta tgcggtgcag gtttaggaag tagttttgca
    21841 gcacagatgt ctgctgagga tgttttgaac aaattaggag tagaagccaa actggaccat
    21901 tgtgatattt cttccgcagt ttccatgaaa ccagatgtga tcatcacagc agaaaatttt
    21961 cgttcgcagt tcgaaaagtt cgcagttgat cctaaaacgg cgatgatcta tttgaaaaat
    22021 attgtttcaa aaaatgaaat tgaagaaaaa atcaccccag ttctgcaaga taagggagtt
    22081 atctaaatac aaaaagaaaa gagggtaaaa acatgggcgt tatcaatttt atcatcgaaa
    22141 atattttgac tcaggcatcg atcaccattg cattgattgc tatgttgggt ctactactgc
    22201 agaagaaatc agcaggacaa gtcatctctg gcacgttaaa gacactccta ggatttcaag
    22261 tgttgagtgc cggttcgagc atcatcgttg gaagtttaac ttatttcggg aaaatcttta
    22321 ctgaaggatt tcatatgcaa gggatcatcc cttctattga atctatcaat ggtcaagcaa
    22381 tgaatgattt gggtttaggt agagatattg cactgacgtt cttagccatt tttgtattca
    22441 atattatttt ggctcgtttc acaaaatgga agtacatttt tttaactggt caagcaattt
    22501 tatggatggc aacgatgacg acagtctttg gctattttgc cggtctgcga gggatcgtat
    22561 tgatcttggt cggtgatttt atcggagctt gtttcgccat tgctatgccg gcagtagcgc
    22621 aaccgatcat tcgtaaaatc actggatcaa atgatattgc tttaggtcat ttttgtacga
    22681 tcgggtactt atttgaagcc ggtgttgcta agctttttgg tgagaaaggc gaaaataaaa
    22741 agtcgatcga agatatcaag ttgccgacac atttcgaatt tctgcaagat acttatttgt
    22801 ctgtcatggt cgtgatggtc ccgctatata tcatcactgt tttatttgca ggcgaaccat
    22861 tcgcttctga attatcagga gatcaaaact atatcatgtt tgccttttta caagccatcc
    22921 aattcgtagt gggtgtgtat gtattgctag ctggtgtacg tctactgtta ggtgaaatcg
    22981 tgccggcatt tcgagggata gctatgaaat tagttcctga tgcgatccct gccttagatt
    23041 gtccagtttt ctttccttat agcccaaatg ctgtcatttt aggtttcatt acgacaacga
    23101 ttggtacgat tattgccatg tttactttgc ccatgttcgg cttagcgatg attttacctg
    23161 gtatgctaac caatttcttt gccggaggga cagcagggat atttggcaac gcagttggcg
    23221 gacgccgagg agcgatcatt ggcggtattg ctcatggttt ctttattaca ttattgcccg
    23281 ctttgctagt aacgatcttt aattcgatgg gattcatcaa tgctacagca accgatgtag
    23341 atacagtagc cgcagcactc ttatatgcct ggatcttaag cccagttttg aaagcatttt
    23401 aattgaaagt agggaaaatg atgtttggat tttttaagaa
//
LOCUS       DACESX010000035        23277 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.035, whole genome shotgun sequence.
ACCESSION   DACESX010000035 DACESX010000000
VERSION     DACESX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.035"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..341
                     /locus_tag="IXF88_001155"
     CDS             <1..341
                     /locus_tag="IXF88_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507895.1"
                     /translation="NKVINPALSINAPLIPTALSFSVTAVVSEIEDTEKIKIVEIEVL
                     NKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVVKFNGTEIGRQIFD
                     IKVNKPVEKN"
     gene            355..756
                     /locus_tag="IXF88_001156"
     CDS             355..756
                     /locus_tag="IXF88_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507896.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            758..1129
                     /locus_tag="IXF88_001157"
     CDS             758..1129
                     /locus_tag="IXF88_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507897.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1165..1467)
                     /locus_tag="IXF88_001158"
     CDS             complement(1165..1467)
                     /locus_tag="IXF88_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507898.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1716..1916)
                     /locus_tag="IXF88_001159"
     CDS             complement(1716..1916)
                     /locus_tag="IXF88_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1507899.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2221..3453)
                     /locus_tag="IXF88_001160"
     CDS             complement(2221..3453)
                     /locus_tag="IXF88_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1507900.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            3706..4275
                     /locus_tag="IXF88_001161"
     CDS             3706..4275
                     /locus_tag="IXF88_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1507901.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4453..4893)
                     /locus_tag="IXF88_001162"
     CDS             complement(4453..4893)
                     /locus_tag="IXF88_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1507902.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5051..5815
                     /gene="map"
                     /locus_tag="IXF88_001163"
     CDS             5051..5815
                     /gene="map"
                     /locus_tag="IXF88_001163"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1507903.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            5847..6770
                     /locus_tag="IXF88_001164"
     CDS             5847..6770
                     /locus_tag="IXF88_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1507904.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            6846..7985
                     /locus_tag="IXF88_001165"
     CDS             6846..7985
                     /locus_tag="IXF88_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1507905.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            7978..8778
                     /locus_tag="IXF88_001166"
     CDS             7978..8778
                     /locus_tag="IXF88_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1507906.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            8778..9605
                     /locus_tag="IXF88_001167"
     CDS             8778..9605
                     /locus_tag="IXF88_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1507907.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9583..10317
                     /locus_tag="IXF88_001168"
     CDS             9583..10317
                     /locus_tag="IXF88_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1507908.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10417..11283
                     /gene="rfbA"
                     /locus_tag="IXF88_001169"
     CDS             10417..11283
                     /gene="rfbA"
                     /locus_tag="IXF88_001169"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1507909.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11297..11869
                     /gene="rfbC"
                     /locus_tag="IXF88_001170"
     CDS             11297..11869
                     /gene="rfbC"
                     /locus_tag="IXF88_001170"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1507910.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            11891..12919
                     /gene="rfbB"
                     /locus_tag="IXF88_001171"
     CDS             11891..12919
                     /gene="rfbB"
                     /locus_tag="IXF88_001171"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1507911.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13017..13868
                     /gene="rfbD"
                     /locus_tag="IXF88_001172"
     CDS             13017..13868
                     /gene="rfbD"
                     /locus_tag="IXF88_001172"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1507912.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            13903..15936
                     /locus_tag="IXF88_001173"
     CDS             13903..15936
                     /locus_tag="IXF88_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507913.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            15980..17260
                     /locus_tag="IXF88_001174"
     CDS             15980..17260
                     /locus_tag="IXF88_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1507914.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17470..18276
                     /locus_tag="IXF88_001175"
     CDS             17470..18276
                     /locus_tag="IXF88_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1507915.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18288..19508
                     /locus_tag="IXF88_001176"
     CDS             18288..19508
                     /locus_tag="IXF88_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507916.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGVNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19498..21084
                     /locus_tag="IXF88_001177"
     CDS             19498..21084
                     /locus_tag="IXF88_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1507917.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21123..23261
                     /locus_tag="IXF88_001178"
     CDS             21123..23261
                     /locus_tag="IXF88_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1507918.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         7415 a         3900 c         4797 g         7165 t
ORIGIN      
        1 ttaataaagt aattaatcca gctctttcta ttaatgcgcc tttaattcct actgctttat
       61 cattttctgt aacagctgtg gtgtctgaga tagaggatac agaaaagata aaaatagtag
      121 aaattgaagt tttaaacaag aatgaaaaac aaattttttc tacgggtgaa gtatcggtaa
      181 gcttgcctcc tcaagttaac gatataaact ttaatataaa cgccagaaat gtcttggttg
      241 aagaagcagg agagcattat gctgttgtta aatttaatgg aactgagatt ggaaggcaaa
      301 tctttgatat caaggtcaac aaaccagtgg aaaaaaatta aggagacgat acggatgctt
      361 gatatagtac catcaaatac acatcggagt ggtagcttaa taaattttgc tgcgattttt
      421 gcttgcgcat cggtaattct agcatctcca gttgttgtgg atgcaagttc tactccgaaa
      481 actaaaaatg ataatcaact tgtaattact acaaaaaatg agattaacac aatttcaagt
      541 aatgatggca aaatttttga tgtgataaat acggttttaa aaagaaaagc tgaaatcaat
      601 ataaaatatg atgaagattt aaacttgtat ttctttgtta tcaaaacaac atctgaattg
      661 ttttcctcag attacgatgt tttggatact ctcgataatg tattatctga ctataaatat
      721 atggggaaat ctgtagtagc aactttgggg gaataaaatg tttgacttta atgaatattt
      781 atctatttcc aaacaaattg aatcagatga taaatataat tctaaagaat cttatagaag
      841 aactgctgtt tcaagagcat actacagtgc ttttaaaaaa tctgatgagt atttgaaaga
      901 aaattatgat attatttata atggaagtag cggaaaagga agccaccaaa ccgtttggaa
      961 tttgttttca acagttaagg aattgaatac cttaggaata cataatagtg ggtatagaat
     1021 gttagagaaa agaaaatgtg ctgactatat tcctaatgaa accattacga aaacagatat
     1081 ggcgctgatg aatcgagaag cagaaaaaat aataaacaaa ctcacctaat gaatttcaag
     1141 cgagtttgtt tgttattttt ttatttaatc tttgtatctt tctggatcaa cgaaagtata
     1201 ctttatatag tcataacgcc gatgatcgct acgtgcgtcc ggcacgtcag tcacgatatc
     1261 aaacaaaaag tatacaccct tcttcattct agttttcgca gcaggaattt tgaaatagtt
     1321 cttattagaa tagtagagat tgattaataa gctatcttcg attgctaaaa agaaaacttc
     1381 tgaattccat actttataaa aatctttgat aaatctattc gaagggtcaa atttaaacca
     1441 taattgtgtt tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa
     1501 tgttcttttt gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc
     1561 aaattatgtg ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat
     1621 attaagattt cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt
     1681 acaaataacc cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta
     1741 gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa
     1801 agtaaaatag atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg
     1861 gaaaaattga ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt
     1921 caatagattc gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata
     1981 tatcgttctt aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac
     2041 tggagatgac tttttattat ttttagtaat agctgatggg ttaatactta caactttcct
     2101 tttcaaagta ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca
     2161 atagcttaga tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac
     2221 tcaagcccaa tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc
     2281 gatatccatc ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc
     2341 agcttctgct aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc
     2401 agaacctagt gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat
     2461 gccagattga gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc
     2521 accttcatct gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc
     2581 aacaactttt ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact
     2641 cagtggcttt gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac
     2701 ttcttccgtc ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc
     2761 ttcccaaaga tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg
     2821 aagggcagaa aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg
     2881 cttcttccat gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc
     2941 ccataatgcc tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg
     3001 tttaccagcc gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa
     3061 attcattagt gctttgccat tcgctgattg ataagacgct actcgatggc tatctacacc
     3121 agcaaatgca tcgggatcgg cagaaacaac gctgatcctg cttgctcctt tttcaagcca
     3181 ttcatccgct tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg
     3241 taaaagaaat tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc
     3301 tagtttataa gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg
     3361 taaaacaact gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata
     3421 tttttttaga agttcattaa aatcagataa cataataaaa attcctcctc accatttttt
     3481 ctggtaacta ttagcatgat accatttaat tatgattcaa acaatgaggc tttaaataaa
     3541 aaaaggaggt tattttttaa ggttaagttt catgatatat gttactactt gctatttcag
     3601 cttgtgaaga aaatcaaaaa ctaattttgc atatcaaaaa aacatgttac aatgaaatta
     3661 atcaatggtg gaagcgttat aaaaaaatta aggagattaa aaaatatggc aagaaaaaag
     3721 acgattacta gagaacagat tttggcagct gcttatgaag tagctgccac cgatggattt
     3781 acacgattta ctgcgagaaa catcgcgaac aaaatgaatt gttccacgca accgatttat
     3841 cttgagttta aaaatatgga tgacttgaga caagctcttt ttgagcagat ctatgatcat
     3901 ttacaatatg aagtgttcac tgttgagcat acagggaata cgattatcga tcttgctttt
     3961 aattatatcc attttgcaag aagagagaag aagttgtatc gttcactgta cttagaagaa
     4021 tatggtggag gaaaagagat gcagaatttc tcttatactt actttgtgaa tacagtgaaa
     4081 cgcgatccag cttatgtgaa tctttcagaa gagcagatcg aatctttgta taatgggaca
     4141 tggatcatag ctactgggat cgcagctttg atgtcttcaa gcatcatcca tccaactgat
     4201 gaacagattg aaaagatgat ccaagacagt atagatgcta tcttgaaatt caaagaacca
     4261 atcgaaatcg attgaatata ctcaaaagtt ccacaaatca gtaggtaaca agagatgtgt
     4321 caaaataagg aaaagttcgt ttttcttaca gcgtatgcat ttttaactcc cgaagaactc
     4381 ttccatgaat gcaatgaata aaagagccat acagattgaa taaatctgga tggctctttt
     4441 attgcaagta tattaaattt ttgcgactag ttctttagcg aaagcttcta aattttcgat
     4501 atcttcttct tcagcattca gatccacttt aacattttcg gctccttttg atgcgcctgt
     4561 ttttgcaaaa gcagcatcga aatcatccac cgatttgcag aattcatcgt aaaaagtatc
     4621 accagatccg cagacaccgt aaatttttcc caatagatcc aattcttgca agtcttcata
     4681 aaagtctacg atctcatcag gcagatcgcc atcatcgtat gtataagtag ctactacgca
     4741 aatatctgcc tcctcgaatt catctgcatc tacttgggta cattcgttga tttcgacttc
     4801 gatatccaga ttttcaaatg cttctgcaac aatatcggcg atttcttcag tgtttcctgt
     4861 catgctagca tatacgattt ttgctaaagc catgatgtgc ctcctcgttt ataattattt
     4921 aacaattatg aattatacca aaacatcatt gaaaagaaaa taaaacctaa agacacctgg
     4981 ggaatgtcat ttggctaaat gtatgccgct atgttaaaat gagagggatt tgtaaaaagg
     5041 agacgaaaat atgattactt taaaatcagc acgtgaaatt gaaatgatgg cagaatctgg
     5101 agctttatta gcagatgtac ataaaaatct gcgtgatttt ataaaaccag gaatcacaag
     5161 ttgggatatt gaggtatttg tcagaaacta tatcgagagt catggcggaa ttgctgcgca
     5221 aattggcttt gaagggtatg aatatgcgac ttgtatcagt ataaatgatg aaatttgtca
     5281 tggattccct cgtaaaaagc ctttgaaaaa cggtgatttg gtaaaagtcg atatgtgtat
     5341 cgatttgaaa ggcggcattt ctgattcttg ttgggcctat gtagttggag aatctacacc
     5401 tgagatcgac cgattgatgg aagtaacgaa gaaagctttg tacatcggta ttgagcaagc
     5461 acaagtaggg aatcggattg gtgacatcgg tcatgctatc caaacttatg tagaaggcga
     5521 aaatctagcg atcgttcgtg attttattgg tcatggcgtt ggtcctacga ttcatgaaga
     5581 accagcagtt cctcattacg gagaagctgg aaaagggttg cggttgaaag aaggcatggt
     5641 tatcacgatc gaacctatgg ttaataccgg aacttggaaa atgaaaatgg atcctaacgg
     5701 ctggactgcg tatacaagag acggcggact ttcttgccaa tatgaacata cattggctat
     5761 tactaaagat ggtccaagaa ttttgacatc acaaggagaa gaaggcactt attgatctat
     5821 ccagtcaata ggtacgggag aggtacatga aagtttggaa caaaataaaa caaaatgaaa
     5881 atctgatgcg ctttattgag acgactcaaa agcgcatcac tgattcagaa atgagtacga
     5941 cctctgtcgt cgttgcttat tatttactct tgtctttatt tccattgatt attgcttttg
     6001 gtaatatttt accgtttctc cacatcgatc aagagactgt actgacctat ttacgagaag
     6061 tgattcctga gaccgtttac cagtttatcg gtcctgcaat caaagacctt ttgacacaaa
     6121 gttcaggagg cttgttatcc gtttctgcat tagcggcttt atggtctgca agtcaaagta
     6181 tcaatgcttt gcaaattgcg atgaataaag cgtatggcgt agaaaataga aaaaattttt
     6241 tgatcgtccg tttcttctca ctaattgtca ttgtcttgtt catgattgct attagtgggg
     6301 taactatcgt tcttgggttg ggacagctga ttttggatgc tattcagcca atcgtacaaa
     6361 tacccgtgaa ctttatcgat cagttccaaa ctttgaagtg gccaatcaca ttggtagctt
     6421 tatttgtcat catgttcttg atttatttaa ttgtccctaa tgctcagttg aagttgaaga
     6481 cgatcattcc gggcacgatc ttcgctactg tcggctggat gctgttatca caagtgttta
     6541 gtatctatgc taggtatttt gcaacgagga tcagcggtta tcagatcata ggaagtttta
     6601 ttgtactcat gctttggctt aattttgctg ctacgatcat cattttgggc ggaatcatca
     6661 atgcagttgt tcaagaatac gtgacgggtc atcaaatcaa aaaacgtcga gatccaagcc
     6721 gaaaatggtt caataaattg aaaactaaat tttctcataa aaaaaagtag aaacaagagt
     6781 tcttattgta aatttatatc agtaatttat aataattatg actcttaatt aaagtaggtg
     6841 cccatatgct tgtttctttt agtatggtta taagattttt tctgacaatt ttattatcac
     6901 tgatattaac acctgtattc aaaatcattt cagtacaaac aggtatggtc gacaaaccaa
     6961 atgaaagaag aataaataaa gttccaatgc cttcagcggg cggactgcct atctttattt
     7021 catttgttat atcgacttta ttcttattcc gtaaaattat ccctcaatcg tatattgtac
     7081 cgatcttgat tgcttcttta gtaatcatag tcacaggagt gctcgatgat aaatatgagc
     7141 tgacacctaa acaaaaaagt atagggatct tgattgcttc tcttattatt tattttgtag
     7201 cagatatacg tattgattcc ttcacgcttc cttttattgg atatatccaa ctaggctggc
     7261 ttagttttcc cgtaacgatt ttttggatct tcggtatcac gaatgcggtc aatctgattg
     7321 acggtttgga tgggctggcg gcagggattt cattgatcgg tttaattacg ataggaatca
     7381 ttggatattt tttcctccat gcttctacag tgtatattcc agttgttatt ttctgtcttg
     7441 tagcaagtat tatcggtttt tttccttaca atttttatcc ggcaaagatt tatttaggcg
     7501 atacaggagc tctattccta gggtttatga tggccgtcct ttcactacaa gggctgaaga
     7561 acgtgacttt tgtttcatcg atttctttgc tgatcgtcat gggagtgccg gtaacagaca
     7621 cgttttttgc tatcattagg agaaaagcaa accgtgtttc tttttctaca gcagataaaa
     7681 aacatcttca tcaccgacta atcagtttag gctttactca taaaggtgct gtcctgacga
     7741 tttatgcgat ggctttgatg ttttctttca cagctatggt catgaattat actggaagaa
     7801 tcggaacgat catattgatt attgccatgt tgtttgctgc aattttatta tttgaattga
     7861 ttggtctgat caatgagaaa catcagttta ttttaaaaac attgcgattt ttagggaata
     7921 aagagtaccg tacgcaagta aaacagaaat atttgaagaa atggaaccgt catatagatg
     7981 aataacaaat taaaccagcc gatccttatt agtattgtga cggcaaacag tgataaaatt
     8041 tttcggacac tagataattt tatttcaact gtaaaagatg aaacagcagt aaaaatccga
     8101 atttttgaca acaattcact tcccgagtac aaaaaaagac taacagagta taggaaatat
     8161 ccctacatcg atatctattt tcatgatgaa aattccggat ttggttatgg acataatcac
     8221 aatctgatag ctagcgattc tcgctatggc attatttgta acccagatat tttagtggat
     8281 gaagagacag ttacagcatt ggtcttgtta ttgaaagaac atccggaatg tgcgatgtta
     8341 gcacctaaga ttcttaatga agatggaaca actcagcacc tcatccgtga gcagctggct
     8401 gtttttgatt acgttttgcg ctttgtgcct ttcaaatggg tcaaaaaaaa gtttgacaaa
     8461 cgactggctg tgtttgaatg ccgaggattg cctactgatg ctaatagtta tgtacggatg
     8521 atctcaggta gttttatggt agctgatatt gataaattta aagagataaa ggggtttgat
     8581 gagcgtttct tcatgtactt tgaagacaac gacttgtgtt tgagttttga aaaagcaggt
     8641 tacaagttat tgtacacacc cttttatgca gttatccatt tttacgggaa gggtgcgcat
     8701 cgaaatgcaa aactgtttcg cgtgtttttg acttctatga tgaaattttt caataagtgg
     8761 gggtggcgtt ttttctgatg aaaccagaaa tattagtagt cgttgtgctt tatcagcaac
     8821 tattcagtca atctccagct tatggaccat taagcaaagc acttagcgaa aagcaaattc
     8881 agcttattat ttacgataat agtcctcaaa ggcagcaaaa tgagctgttt gaaaaagaaa
     8941 atatcgttta tcatcatgat ccgcaaaatc ctggtttagc ggtggcttat aattttgcgt
     9001 tgtctcaagc agatgaagga acacgttgtc tcgtcactct ggatcaggat acgaggctag
     9061 tcgacgatta ttttgaacta ttgcgtaaag taacctttac agatgaatgt gttgctgcag
     9121 ttccgatgat tttttctggg agccgacaag tttctcccct atattcagat cgttatatca
     9181 atcgtacagc tcaagcagta gaagttgaag gcccaacagc tgaacgaatc atggcaatca
     9241 attcaggtgt tgcctggtct gtcaaatttc taaaaaagat tggcgggttt aatcctgctt
     9301 tttctttaga ttttttggat cattggctgt tttggaaagt ggatcagttg gaaaaaactg
     9361 tggagatcct tcctgtacga atggaacatg atctttcggt tttagactat gaaaaagttt
     9421 caagtaatcg ataccgttct attttacaag cagaaaatcg attttatcat gaatatgatc
     9481 aaaggcattt atccagtcac cgacgacaat tattgttaag aacagctaaa cagttcttga
     9541 ccgtcaaaaa ccggcatatc tggcggatga ctttgcgctc atatgttaag aattggaagg
     9601 tgtgaattcg tgcgttcggt ttgtatagct acctttaatg gaggcagata tttaagagag
     9661 cagttagata gtattttatc tcagctttca gcagaagatg aagtagtcat ttctgatgat
     9721 gggtcagtag atgatacatg ggatatatta actgaatacg cagaaaaaga taagcgaatc
     9781 cagctttttt cggggcctcg tcaaggattg atcgctaatt ttggttatgc gatccagcag
     9841 acgaaaggag atctaatctt tttggcagac caagatgatg tttggttgga gacaaaagtg
     9901 aaaaagatca ctgactattt tgagcagaat ccggatcagc tagtagtagt ttctgattta
     9961 atcattgtgg acagcgagct gcaagagatc catgattcgt atttttcgtt tcgaaattcc
    10021 aaagaaggat ggcttaataa tgtgataaga agtcactata ttggtgctgg tatggctttc
    10081 cggtcttctt taaaacccga tattttgccg atcccgtcag aagtgccgat gcatgatatg
    10141 tggattggtt tgctgggagg aaagaagact gggttcctaa gagaaccgtt aactctgtac
    10201 cgaagacatg atttaaacgc gagtgagatt gaaacacatt ctaaattacg gcaaaaaatt
    10261 tcttggcgtg ttcatctgat tacggcgttg atcaaacgaa aatggtttaa acgttgataa
    10321 aagatgttta aatccttaat atttccatga caaatataac aattgatgag ataataacaa
    10381 agtgaaattt gaaatcaaaa aggagagcag atatccatga agggaatcat tcttgctggg
    10441 ggcagcggaa cacgtctgta ccctttaaca aaggcaacat cgaaacaatt gatgccgatt
    10501 tatgacaaac caatgattta ttatccaatg tctactctga tgttggcggg aatcaaagaa
    10561 attctgatta tttcaacgcc acaagataca ccacgtttca aggaattatt tggaaacgga
    10621 aatgatttag gactgcagat cgaatatgca gtacaagaaa gtccagatgg attggcacaa
    10681 gcgtttatca ttggagaaga atttattgga gatgatagtg tctgcttggt attaggggac
    10741 aatatttact atggtggcgg tctgtctaaa atgttgcaac gagcagcttc aaaagataca
    10801 ggcgcgacag tatttggcta tcatgtcaat gatccagaac gctttggtgt agtagaattt
    10861 gatgagaata tgcaagcttt gtcgattgaa gaaaagcctg ctcagccaaa atcaaattac
    10921 gctgtaactg gcctttattt ttatgacaat gaggtaattt ctatcgctaa aggtatcaaa
    10981 ccttcagaac gtggagaact agagattacg gatgtcaata aagcatattt ggataaagga
    11041 aaactatccg tagaacttat gggacgcgga tttgcttggc ttgatacagg aacacatgaa
    11101 tcattgcttg aagcatctac ctttatcgaa acaattgaaa aacgtcagaa cttaaaagtg
    11161 gcttgtttgg aagaaattgc ttaccgtatg ggctacatcg ataaggaaca attgctggcc
    11221 cttgcacagc cattgaaaaa aaatcaatac ggtcaatatt tattgaattt ggccgctgaa
    11281 taaggagtcc ttacagatga aagtaacaga tacaaaatta acagatgtca aaatcgtaga
    11341 aatggacgtc tttggcgatc atcgcggttt cttcacagaa agttattcaa aagcaaaatt
    11401 tgcagaacat ggattgaaat atgactttgt tcaagacaac cattcactat cagctgaagc
    11461 tggtgtcctt cgggggcttc atttccaaaa aggagaagct gcacagacaa aattgattcg
    11521 ggtagtcact ggagcagttt tagatgtagt agtggatatc cgcaaaggta gcccgactta
    11581 tggtcaatgg gacggattta tcttatctga acataatcat cgacagttgc ttgtacctaa
    11641 aggatatgcc catggattcg ttactttgac accaaatgtg aacttccttt ataagtgtga
    11701 taattactat aatgcagaag cagatggggg catcgcattt gatgatccag atttagcgat
    11761 tgattggcca attgatccat caagagcgat tatgtcggaa aaagatcacc gtcatccgac
    11821 attaaaagaa tttgaagcag aaaatccatt tgtttacgga gaaatctaag aattaaggag
    11881 aaattttctt atgaaaaaca tcattgttac cggaggtgcc ggattcatcg gctctaattt
    11941 cgttcattac gtggtaaaca atcatccaga agtgcatgtc actgttttgg ataaactgac
    12001 ttatgcagga aacaaagaga acttggccgg acttcctagc gatcgcgtag aattggtcgt
    12061 aggagatgtt gcagatgcag aattagtgga ccgtctagta aaagaagcgg acgcagttgt
    12121 tcattacgcg gcagaatccc ataacgataa ttcattaaaa gatcctttcc catttgtaca
    12181 aacgaatatc attggtacgt atacactttt agaagcttgc cgcaagtatg atgtacgcta
    12241 tcatcacgta tctactgacg aggtttacgg cgatcttcca ttacgtgaag acctgccagg
    12301 acatggggaa ggcgaaggag aaaaattcac tgcggaaaca ccatataatc cttcaagtcc
    12361 atattcttct acaaaagctg gttcagatct gttagtaaaa gcatgggtgc gttcttttgg
    12421 cttacgtgca accatttcaa actgttcaaa caactatggt ccttatcagc atattgaaaa
    12481 atttattcct cgccaagtaa cgagtatctt aagcggtatt cgtccaaaat tatatggcga
    12541 aggaaagaat gttcgtgact ggatccatac gaatgatcat tcttcagcag tgtggctgat
    12601 tttgacgaaa ggcagaattg gcgaaaccta tctgatcggt gcagacggtg aagaagataa
    12661 taagaccgtg atggaattga tcttagaaat gatgggccaa ccaaaagacg catacgacca
    12721 tgtaaacgac cgcgcagggc acgatttgcg ttatgcaatc gattcgacaa aattgcgaga
    12781 agagctaggt tggcagcctg agtttacgaa cttccgtgat gggttggctg acacgatcaa
    12841 atggtacgaa gaacatgaag actggtggaa aaaagaaaaa gaggccgttg aagcagcata
    12901 tgccaaaaat ggacaataat acagaggaaa tttcactgta ttaatggaaa aacacatgtg
    12961 aggcaggcgc tcgcctgtct cttttttaaa ataaaaatca aggagcggat aaccatatgt
    13021 ttttattaac tggaggaaat ggacaattag gtacagaatt gcgccattta ttagatgaaa
    13081 aaaatctgcc atatgtttca acagatgcaa aagacttgga tatcacagat gctgagaaaa
    13141 caatggctta tattacagaa ttaaaacccg atgttatttt ccattgcgca gcttacacag
    13201 cagtagataa agcagaagaa gaagcaaaag aattagatga gaaaatcaat gtcgatggca
    13261 caagaaatgt agcacttgca gcaaaagctg tgaatgcaca attcgtctat atcagtacag
    13321 actatgtttt cgatggaaaa aacaaaaatg tagaatacga agtagacgac caaacgaatc
    13381 cactaaatga atatggtcgt acgaaattat taggcgaaaa agcagtagaa gagattttag
    13441 aagactatta tatcatacga acttcttggg ttttcgggat ctatggacac aatttcgtct
    13501 tcacgatgca acgcctagcg gaaacccgcg acaaactgac tgtagtaaat gaccagtttg
    13561 ggcgaccaac ttggacacgt actttggcag aatttatggt atatgtcatc gaagaaaaag
    13621 caccatttgg tatttatcat ttgtcgaacg aaaatagctg ttcttggtat gagttcgcaa
    13681 aagaaatatt gaaagataca gatgtagaag ttgcacctgt tacgtcagaa gaatttccgc
    13741 aaaaagcaac acgtccgcag tattcagtca tgagcctgaa aaagacagaa gcgttaggtt
    13801 ttgtcatacc aacttggcaa gaagcacttg cgcaaatgct ggaagctgcc aaaaaaatga
    13861 ataagtaatt tcctttttat caaatagaat ttggagcatg atatggaaaa acaaacaaga
    13921 agtgcacgac gaaaagaaaa gccaaaagcg aatcaggcag attcgaattt ggtgcttgac
    13981 ctgtttctaa aactccgcta tcttattggc gttttagtca tcgtggtcat cgttactttt
    14041 aatttaaacg gaagttcact tggtgtttgg gataaatatg tgtcgcaaag agacgatggg
    14101 aaaaaaactg atgtgatttt tggacagaat cgggaagtgc ggtcagacga atggctggtg
    14161 cagacacctt tttacctctc acaagctgaa gaggactttt cgttggtgaa cgaggattat
    14221 tcattaaacg gacaaaacat gatcattgcg tataattctc ctgtaaagga tattacggtt
    14281 atcggaaaac cattcaactg gggattcttt ttcctcggaa gagatcgagg actttccttt
    14341 tattgggcga tgaaattagt cgtcatggtc ttgcttggat tcgaagtact gatgatatta
    14401 acgaagagga aaaaagctct atcgctaatc ggtgcatttg cgctgacatt ttctccagca
    14461 gtccagtggt ggttcatgca acatgtgggc gatttgattt tctttacttt gggactgatg
    14521 attgcttttt atcattattt ctacaatcat gataaaaaat ggctacgaac gttgatgatg
    14581 cttttggtgg ttattttcgg tattgggttt attttagtga tttacccagc ccatcaagtg
    14641 atgctggcgt acttgctgat tttttatttt gttggcctgc ttatttacta tagcaagaaa
    14701 gtttcatggg atctgttgga tgtagtgttg attggagggg cagttttatt tattggcgga
    14761 gtcatggttc atttctggct aacgtcaaaa gacgcattgt tagcttcttt gaatacctta
    14821 tatccaggaa aacgggtaag tactggagga aagtggacga ttggcgaatt tttttctttc
    14881 cttacaaatt ggaaaattcc ttttgaagat atcaactatt cgaataactc tgaagtggcc
    14941 ctgttctatc acttttttcc aactgtattc ctagcaagtc catttgtgtt atttggcaaa
    15001 aaagatagcg aacaaaagtt actgggacgt attttgatga ttttttgtct gttttctatc
    15061 ttttggatta ccgttgggtt gccaaaatgg ctagcagaaa tcactctgct gtcttatgtt
    15121 cctccattaa gagccatcct tacttttagt tttgcggctt gtttattatc gatctggttt
    15181 atcgcttata tttggagaga agatgttatt cctatttggt ataaattcat tttgttggca
    15241 gtaaatgcat ggtcttattt ctatgcgatt acgcattcga aaatggataa ttatttttcg
    15301 gattttgaaa ccatcgcagt tgctgtgttg ggaacagtga ttttagcttt tgcattattt
    15361 ggctggagac gcttgtttta cctcgctttt gtcgccttag ttatcgtatc tggctttttc
    15421 gtcaatccag tcgtagaagg tacaggagcg atttttgaaa aaacactggc aaaagaaatc
    15481 caaaaaatcg atcaacaaga tcctggtaaa gtttggttgt ctgaagatga attatataac
    15541 ttcaccccaa cattgggagt gaaatccttc aatacagtac gcttttatcc agatatagct
    15601 gcatggaaga aaatcgatcc gaaagaagaa tacgagaagt attataatcg gtatgctcat
    15661 acaagagctt ttatcgctaa ggaagataca aagttcattt tagatcgtcc agatatgttt
    15721 tcggtcacgt tgaattttga agacgcagaa aaattaggga tcgactatgt gatcagtaaa
    15781 cgaccattaa ctgattacaa ccaattatat gaagcgcagt tcacgcagct atatggaccg
    15841 gataaagacg gttatatgat ttttaaagta gaatatccgg attatccaga tgtaccacaa
    15901 acacaaactc aggaaaatca gctagtcgta cagtaatagg ctagctgaga ataatcctga
    15961 aatattagta gaaaggagag tgaagataat tcaagcaatc gtacaaaaat ttgtcaacaa
    16021 aatgagtgtc ggtttcttgc ttttgttgct gacgctagtc ttttttgttt ggacagcagg
    16081 ggtaggtttt ccagcaactt tgtttatata taacaatagt tcattactta tcgcttttag
    16141 tgtggctatt ttgctcattt taaatgttta taaagcaaaa ggcgtcgatt ttctttatct
    16201 aggtgttgcg cttgcccttt tcattttctt ttcttacttt cgggaattaa gaaatgagtc
    16261 tttatttgga gacttactgt ttcctttgat tattttattt tgtttagtaa ttaaatggat
    16321 cgagtggaca aaatgggatc gcggtattta tctgattgtc tttacggtct tcctcggaat
    16381 cacgttatat cgtgtattta cagagatcca agtaccagaa ggacaaagca tctgggctcc
    16441 aggcaattcg ctttctgata tttggatcaa tacgaataca atcggaagtt ctttgatgac
    16501 tcttgcttta ttgatcagtg gatttgccag ttcttttgaa cgatggtatg ttcgaatcat
    16561 cggtgggata gcagtcgttg cagccttttt aggtatttgg gtctgccaat ctagaggtgc
    16621 attagtcgcg ttgattgttt ttgcgatatt ggatctttta cctaaatcgt tgatgcgtgt
    16681 cattcgtgcg ccatttattg cctacacggt tacgattctt ttagctttgc cgatttctta
    16741 tctggcggct gtctctgaaa aagtcaattt gtttaccggt cgagaagata tttggcataa
    16801 attctatcag acgctaggag aaaaatctga acaagtttta ctaggaatga aaacatttat
    16861 cttccagcga ggaaaccaat ttttaggaaa ccacaatagt tataattcta ttttgaatat
    16921 ctatggattg ataggatttg ggattgctgc tttgctgctt atccttttta ttggtcgctt
    16981 aacgttgaaa gcagatttat ccaacgggca gatgactttt atctgggcct tttttgcggt
    17041 gatgatgcag tcatttatgg aggatacatt gacttcatac cgatgggttc cgattattta
    17101 tatcctgttg gctatggccg ttcatcgata tgatgaacca gaagttacgg aggaattcta
    17161 tgttatggaa gaggcatatg acgatatgac ttatgatgat acatcttatg aggaacaacc
    17221 agatagcgct cattcttcca ggatccaacg ctatcattga taggcacaaa atacgagaga
    17281 ggctgtgaca accgtcgcag ccttttttgg tttattgcaa ttgtctagca tcttctgtaa
    17341 aatagaaacg ttagagaaaa tcttgtatca tagaaaatta agaaagaaat aagaacgcag
    17401 ctcggtacca aaaatatggt aaaatggcac ttgactgtaa ttcactgaaa atcgcttgga
    17461 ggaacacaaa tgtttaaaga tttgtattta tttatgaagg acatttatca gaacagaaaa
    17521 ttactgctac agttttcgat caatgacttc aaatcaagat atgcaggatc gtttttagga
    17581 atcttatggg cgtttatcaa tccgctattt actgtattga tttattggct ggtatttggt
    17641 gttggactga aagcacaaat gatcgatgca aaatacccat ttatcgtata tttagttacc
    17701 gggatgattc cctggttctt cttctcagat gcatttgcat caactacggt tgtttttcgg
    17761 gaatatactt atttagtaaa gaaagtcgta ttcaatattc gtatcttgcc cacgacgaaa
    17821 atcctatcca atctgtacac ccatttattc ttcattctga ttggcatagc tgtcgcgctt
    17881 ataaatggta tatatccttc agcaatgtct ttacagctga tttattattt attttgcatg
    17941 tgtgctttct tgacaggatt gacttggctg acggcatcga tccaaccatt tttacctgat
    18001 atcatgcaat taattacgat cgtgctgcag ttgatcatgt ggacattacc gatattatgg
    18061 agccctaatc agtttgattc aggtcttgta agcatattga aattaaatcc gctgtactat
    18121 gttgttcaag gttatcgtga gtcattttta agtgaagctt ggttctggga gcatggaaca
    18181 tatactttgt atttctgggt tttcacgtta gtaatgttcg taatcggctc tgcagtgttc
    18241 cgtagattga aaccacattt ttcagatgtg ctgtaaggag aatagctatg acagaatatg
    18301 caattagact aaaagatatc acaaaatctt ataatatgta cgcaaaacct tcggatcgat
    18361 ttcgtgaagc cctcaatcct tttaagaaat cctatcatga tgtattttat gcgttgaaaa
    18421 acgtgaatgt agaaataaaa aaaggtgaga tgatcggttt cgtgggagaa aacggatcgg
    18481 gaaaatctac aatgttgaaa atcattacgg gagtactgac accaacatcc ggcacgatgg
    18541 aaatcgaagg aaaaatctca gctttgctag aactaggatc aggagtcaat cctgagtatt
    18601 ctggctatga aaatatctat ttaaatggaa tggttctagg attttcaaga gaagaagtcg
    18661 atgaaatggt cgatgatatc attgaatttg ctgacatcgg cgaccatatt tatcaacctg
    18721 taaaaaccta ttcaagtggg atgttcgttc gtctagcttt tgctgtagcg atcaatgtga
    18781 atcctgatat tttgattgtg gatgaagcat tggcagtagg agatcttgaa tttcaactaa
    18841 aatgtatgga aaaatttaca gaaatcaaga actcgggaaa gacgatttta ttcgtttctc
    18901 atgatattag ttcgattcgc cgtttttgtg atcgttccta ttggttgaaa aatggaaaag
    18961 ttgtcgaata tggtgatacg atggaagtaa cgacgaatta cgaaaacttt ttgaagaaaa
    19021 aatcagttaa aacagtagat cgaaataaaa accaagtaga acgcttcgca gctccagata
    19081 tcgtagacgt gacgaaagcc gaattgttgg ataaagataa aaatccagtg gatatgctgg
    19141 aacaaggaga aaaactatac gttaaagtaa cctatgaagt aaaagacgat acaatcaaaa
    19201 atccagtttt aggaattgct ctgcgtacag tcgacaattt ttatgtgtgc ggactcaata
    19261 ctctactaga tggtatcaag atcccttgga aaaaaggtga gaatatcttt tatctggagt
    19321 atgaaaagtt aggtttgtta gctggtgaat attattttga cgttgcggtc tttgaagaaa
    19381 atgcaacggt tcctttggta tacaagacaa aatacatgaa cttgtttgtc agcggttctt
    19441 atatcggaga aggcatcgtg gtcttagacc ataaatggga agaagggact catagcaatg
    19501 aaatatgatt ttgaaatgga cctagatgaa caaagcagtg tgggaaaaat tgccgcacag
    19561 atcaaaccag gaagtaaagt tttagagttt ggtcctggaa acggccgatt aacgaaacat
    19621 ttgatcggag caaaacaatg tgaagtgagc attgtcgagc tagataagga attgttcgat
    19681 tttgtcagcg agtttgccca agatggattt tatggagata tcgaaagttt tgaatgggca
    19741 aactattatg ctggacaaac atttgattac gttttgtttg ccgatgtttt agaacatttg
    19801 gtagatcctg gaaaaacgtt gaagaaagtt agagaatttt tgaatgaaga aggagaaatt
    19861 ctaattacat tccctaacct tgcgcataat tctgtcatga ttgatttgtt caataatcaa
    19921 ttgccatggg cttcttatgg tttgctagat gaaacccata attcttttta tactcatgat
    19981 ggcttccaaa aagtttttga aaaagcagga ttatacatca atattgaaga ttatttgtac
    20041 ttagctgtag gtgatacaga attaaaatca acttatgaag aattgccgga agctgtacgt
    20101 tatgatttca aaatgcgacc atttggcgaa gtctaccaat acttcttctc attgatgaaa
    20161 catccagtag cacaaagttc cattgcgcag ccgcaaaatt ctaattatgt gaaagtatta
    20221 gaagtaatcc agcaaacgaa acaagatgaa ataattcaac aaataccatt caataatttc
    20281 acaggggaaa atgagacact aagtttccat gtatcagaat tagcagaacg aatggtgttt
    20341 cgcttcgcgc agcaacctag ctttatcgaa ttcagcgcag aagctgctgg agaaaaactc
    20401 acatttatcg attcaaatgc tgtagtcaaa acagtaaatg attgttattt gtttgatggt
    20461 aaagatttac cagagttctt tttaacggat attgcaggga aagaagtgac tatccattgt
    20521 cactatcgtt ttatcggaga attgacacca acaatgaaag aattgctgga aacaatccgt
    20581 ccaatggctg aagttacaaa gcagctttct gagaaaaatg atgaattaga aagagaaaat
    20641 cattatctta ttgagaataa tacaagatta gatcagacgt tgaaaacaac cataaatcgc
    20701 tattgcacgt tgttggaaag tgatgaatgg acaatcaaac atagattagg gcgacgtaag
    20761 aaagaaacat cgaaaaaaat ccaagaaaaa gagctttctc tatgtattga cgaaaaaatc
    20821 tgggatgcag aaacaaagat attgaaaatc atcgggtggg gaatcgctaa ttcggatcgc
    20881 caaccattgt cctataagtt atcagccgac caatcaccat tttttgaagc aattcctgtt
    20941 ttacgagaag aagtcaatca agcaaaacag ctagctgaag gagcaaaagc aggatttgaa
    21001 ctgcggatcc tttgcgagaa ggaacgttca ttcttagttg aagcagtggc tcaaaatggt
    21061 caatcatgga tcattgaaat gtaaataatg attaacttat aaagataagg agtaaacgaa
    21121 tcgtgcaaaa aaaagaaatt gccgtattta tcgatcaaat cactcgtgaa cgggccactg
    21181 gcgatttgct tattgtgggc tgggcaattg atgaagtgac caaagaaatc cctacgataa
    21241 aggtagaaaa agaaaatgtg attgctgaag caacacatgt cgtaagattg gatatcaatc
    21301 atttatataa tcttgatgtt aagacccaaa gcggttttaa ggtacgttta tcagggaaaa
    21361 tgagaggaaa agcaatctta gacttccaaa cagcaagaca tcaaaatggc atagctgtga
    21421 aattaaacgg taaatatcct tatgatgacg gcattgaatc ttcatgggaa agaaaaaaaa
    21481 gattactgaa aaaagggata aattatgcaa ggactcatgg cgtaaaaaaa gcaatccgcc
    21541 gggtcaaact ggaattgaaa ccaggatcga tcgactatgc agagtggatc agtcgtcatg
    21601 agaaacctga tctgaaagag caaaggaaag aagtacaagg atttgcctac cgcccattga
    21661 tttccatact tatgcctgtc tataacgttg aaatcaaatg gctcgaaaaa tgcattgatt
    21721 cggtacttga tcaaacgtat gatcactggg aactatgtat cagtgatgat gcttctactg
    21781 atccagctat ccgaaagtgt ctagaatcct atcaagcaaa agatgataga attaaagtcg
    21841 ttttccgaca agaaaatgga cacatcagtc tggcaactaa ttctgcattg gaaatggcag
    21901 aaggagagtt tatagcgctc ttagataatg atgacgaatt acctccattt gccttatatg
    21961 aagtggcaaa agtgctaaac gtacatccag aactggattt gatctacagt gacgaagata
    22021 aaatcgatgc agatgggaat cgctttgacc cgcacttcaa agcagactgg tctcccgata
    22081 cattgatggg aaataattat atctctcact taggcgtata tcggacaagc attgttaagg
    22141 aacttggcgg tttccgtaaa ggatatgaag gctctcaaga ttatgatttg gttcttcgtg
    22201 tgacagaaca aattcctgca gaacacattt accacattga ccgtgtcttg tatcattggc
    22261 ggacgatccc gggatcaact gcaagcaatg gagaagctaa aagctacatc tatgattctg
    22321 gagtgaaagc attgacagat gctctatcta gaagaaatat caagggaagc gtacatcctg
    22381 gcagaatttc tggtttttat gaaatcactt atgatgtttt gcaagaagac ttggtcagtg
    22441 tgatcattcc aactaagaat ggctatgaag atctaaaaac ttgtgtggat tcgatcatcg
    22501 aaaaaacgtc ttatccaaat tatgaaatca ttattgcaga taatggaagt acagatccta
    22561 aaatgcaaga attatttgca gaatacaagc atcagctgaa agaccgattt atcgtcgaat
    22621 taattgacat tccattcaat tattcacgta tcaacaatct ggcagcagaa aaagcaaacg
    22681 gcaaatactt tttattcttg aataacgata cagaagtgat tgaacctgac tggatgacag
    22741 cgatggtttc ttatgcacag tttgatcgaa tcggctgtgt aggagcaaaa ttattttatc
    22801 cggatgatac aacgcagcat gcaggtgtat tattagggat cggtggcgtt gcaggacatg
    22861 cattgaataa ctatgatcga acacattgtg gttactttgg ccgattggtc attgacgtca
    22921 attacttagc agtcactgca gcttgtatga tggtcaaggc agctgacttc aatgctgtca
    22981 atggatttga tgaaatatta gaagtagcat tcaacgatgt tgacttgtgc ttgaaagttt
    23041 atgaactggg tcgttacaat gtctatgcac atcaagcaga attgtaccat ttcgaatcta
    23101 aatcaagagg ttatgaagat acaccagaaa aacaaagacg ttttgctggg gaaatcaaaa
    23161 aaatgcaaga taagtggcct tcttatattg ctcatgaccc attttataac gataatctaa
    23221 ctaaagaagg aatcggcgat ttttcgttga gacctgattg attaaaagaa ctgtatt
//
LOCUS       DACESX010000036        22967 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.036, whole genome shotgun sequence.
ACCESSION   DACESX010000036 DACESX010000000
VERSION     DACESX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.036"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            103..1068
                     /locus_tag="IXF88_001179"
     CDS             103..1068
                     /locus_tag="IXF88_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1507919.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1125..1355)
                     /locus_tag="IXF88_001180"
     CDS             complement(1125..1355)
                     /locus_tag="IXF88_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1507920.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1441..2328)
                     /locus_tag="IXF88_001181"
     CDS             complement(1441..2328)
                     /locus_tag="IXF88_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1507921.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2417..3640)
                     /locus_tag="IXF88_001182"
     CDS             complement(2417..3640)
                     /locus_tag="IXF88_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1507922.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3657..4736)
                     /locus_tag="IXF88_001183"
     CDS             complement(3657..4736)
                     /locus_tag="IXF88_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1507923.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(4867..6594)
                     /gene="ptsP"
                     /locus_tag="IXF88_001184"
     CDS             complement(4867..6594)
                     /gene="ptsP"
                     /locus_tag="IXF88_001184"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1507924.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6594..6860)
                     /locus_tag="IXF88_001185"
     CDS             complement(6594..6860)
                     /locus_tag="IXF88_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1507925.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7339..9573
                     /locus_tag="IXF88_001186"
     CDS             7339..9573
                     /locus_tag="IXF88_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1507926.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9721..10041
                     /locus_tag="IXF88_001187"
     CDS             9721..10041
                     /locus_tag="IXF88_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1507927.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10246..10470)
                     /locus_tag="IXF88_001188"
     CDS             complement(10246..10470)
                     /locus_tag="IXF88_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ1507928.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10668..12248)
                     /locus_tag="IXF88_001189"
     CDS             complement(10668..12248)
                     /locus_tag="IXF88_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1507929.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12649..14025)
                     /locus_tag="IXF88_001190"
     CDS             complement(12649..14025)
                     /locus_tag="IXF88_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1507930.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14153..15271)
                     /locus_tag="IXF88_001191"
     CDS             complement(14153..15271)
                     /locus_tag="IXF88_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1507931.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15413..15847
                     /locus_tag="IXF88_001192"
     CDS             15413..15847
                     /locus_tag="IXF88_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1507932.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15925..16458)
                     /locus_tag="IXF88_001193"
     CDS             complement(15925..16458)
                     /locus_tag="IXF88_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1507933.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16552..17730)
                     /gene="mutY"
                     /locus_tag="IXF88_001194"
     CDS             complement(16552..17730)
                     /gene="mutY"
                     /locus_tag="IXF88_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1507934.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17723..18520)
                     /gene="recX"
                     /locus_tag="IXF88_001195"
     CDS             complement(17723..18520)
                     /gene="recX"
                     /locus_tag="IXF88_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1507935.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18627..20009
                     /gene="rlmD"
                     /locus_tag="IXF88_001196"
     CDS             18627..20009
                     /gene="rlmD"
                     /locus_tag="IXF88_001196"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1507936.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20183..20761)
                     /locus_tag="IXF88_001197"
     CDS             complement(20183..20761)
                     /locus_tag="IXF88_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1507937.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21083..21553
                     /locus_tag="IXF88_001198"
     CDS             21083..21553
                     /locus_tag="IXF88_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1507938.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21661..22887)
                     /locus_tag="IXF88_001199"
     CDS             complement(21661..22887)
                     /locus_tag="IXF88_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1507939.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         6813 a         4645 c         3955 g         7554 t
ORIGIN      
        1 tcaaagtatt ttctgtgaac agaactatag aattccctaa aacttgtcca ttatttcaaa
       61 atttgttata gtctttttat tcaaagcgta aaaggagatc ctatgttaaa agaaaaaatg
      121 aaaactctcc ccatgctgcc gggtgtctat cttatgaaag accgagatgg taaggtcatc
      181 tatgttggta aagcaaaacg gctcaaaaat agagtaagca gctattttca tcagaataaa
      241 cagcattcaa aaaaagtatt acgtatgatt catcatataa cagattttga tttcgtcgta
      301 gtagacacgg aattagatgc tttattgctc gagtgtcaat tgatccaaca ttaccgtcct
      361 ttttataacc gacaaatgaa ttatttcagt aattataatt atgtacatat taccaataag
      421 ggatttgtat taacagatac tcctacggct cgaacatacg ggccttttcg tctctataaa
      481 aaaatgccgt ctattttgcg tataatggaa gaaacttatc aaatgccttg gttgtctgaa
      541 attagtttgt tagctttgcg agtccagctt cctgacctac aggaaatgac ttttgaacag
      601 aaaaaaaagg aactccaagg gctgtttcaa ggccgaaata aaaagctact gacttacttg
      661 aaaaaaagac aacaacattt catctatcag ttgaatttcg aaaaagccgg tatgcttcaa
      721 aaagatatcg aattagttac ttattttatt agacggatcc aggaacaaaa gcagttttta
      781 agaacaccat cgctcacctt cagcatgcca ttagcagcag atgagagtca gaaaaagcac
      841 tacctcgttt gttatggtca acttgcagaa acaatgatta cctctggtga cgtttctcct
      901 gacttctatt atgaaaagaa agaagctcat ttatctttga aacgtcaatt gtcaaaagaa
      961 gaaatcgatc ctgtacaaat cttgatcagt taccgcaaaa aattagaaaa agaacagttt
     1021 gaaattgaac tgttaaacaa aaaagaagct gaaaagcaac taaattagtt gcttctcggc
     1081 ttcttttagg catcttcaac ttcaaatgtc aattatccta aaatttattg gattaagtca
     1141 tagatttcca tcgctacgat atcgatatta tcaaattcgt attgctgatg agtatctgtt
     1201 tcagttaatt caaatgtatc ggtcgcttta tcataagtta ctgtacactt ctctacgcct
     1261 tctttttcaa aacggcgaac ttgcagttca ttatctgctg actcgaccat agcctccagt
     1321 cgttttatga ttgcgactaa ttgtgaatgt gtcatcggag gtcctccttt tcttatttct
     1381 gttatattgt atcaaaagca tagcctactt gcattgattt tctctaaaaa aactcgtttt
     1441 ttatgatttt ttttctggta tttttccttc aggccaccaa agtttgatca aagcttgggt
     1501 tccgtcattc tcaaaacctt tatctgccaa ctcttcatat agtttcaatg cttgggtagt
     1561 tgctggcaat acaagatcca ttttttctgc ttcttctaat gcaatcttta aatctttgat
     1621 aaaatgtttg acaaaaaaac caggtgtgta atcttctttt aaaatacgag gagaataatt
     1681 gcttaaagac cagttagctg cacttccacc tgaaagcgtc tcaatcactt tctttaaatc
     1741 aagtccgttt ttttgtgcat agacaagcat ttccgtcatt cctgtcatcg tacctgcaat
     1801 catgatctga ttagccattt ttgtatgctg acctttccca gcagaaccat gcaatgtaaa
     1861 tgtcgtacca aattctttga ataaaggcag tacttgatca taaacggctt cttctccacc
     1921 gaccatgatc gtcagtgtac cattctttgc tcctaagtca ccaccggata ccggtgcatc
     1981 taacgcatct gcgccttttt cttttgctgt ttttgcaatt ttttctgcca ggcttggtgt
     2041 acttgtagtc aaatcaacta aaatatgtcc tgagatatct gcagaaaaaa tccccgactg
     2101 tccatagtag atttcttcta catctttggg gtaacctacc attgtaaaga gaatattact
     2161 ttgttctgct accgcttttg gtgtatctgc ccagatagct ccttctgtta ctaaaggatc
     2221 agcttttgct tttgtccgat tatagacagc tacttcgtgt cctgcttcta gtagatggcg
     2281 tgcgacagca cttcccatga cgcctgttcc gataaaacca agtttcattt tttcgacctc
     2341 ctaaaaatca aaaatccttg cttctatgct agtatacgct agaaacaagg attttttcat
     2401 aaaaaatgtt tgaattttat cttttaaatg aatttaattt tattttgatt cgttcaatcg
     2461 agtcttcttc ttcttttctt cgcatctctt cttcatgata aacaaccatg tcttgataaa
     2521 attcgatcat agcttcgccg aaatgtgtag atgaaatatc ataaagttta gcttctaaaa
     2581 cttttggatc tttaggaact tcactggaaa tataatcaat aagtgctggt gcaaagtctt
     2641 cgtcggtttc aaaagttttc cctaatgaag gatgatcgaa caatcggttc aaataagcat
     2701 ttccttccac gacacaaggt gttccggctg ccatcgcttc tgtataagta agcccttgtg
     2761 tttctgaagt agaagcactg acaaaataat ctgccgcttt ataataaagt gctacatcat
     2821 tgttgtccac ttctcctgtg aactgcacaa attcttctaa ctgcagttct gagatcaatt
     2881 cctctaactc ttctacataa ggtccttttc ctacgataac taatctggcg ttaggataag
     2941 cggagatgac ctgaggaaac ccattaatca aggcttggat atttttctca taagaaatcc
     3001 tgcttaaaga aagaatcatc aaatggtttt cctgaagacc tagtgactcc cttaatttgc
     3061 tgatatcctg ctgagtgata tcagaacgtt tgaatttttc aatgtcgata ccagtaggaa
     3121 tcacgcgaag cggtgctatt acaccatagc tgcgtaatgt atcaataaca cgttcacttg
     3181 gacaaactac acctgtcgta tgatttgcaa ataatctaga caagtatttt acatgggatt
     3241 gcctgatcac ttttccttta ccgatataat gaaggtagtc ttcatacatc gtatggtaag
     3301 tatggatcag aggtattttc aacttcttag ctaccatctt cccaagtatc cctgctccga
     3361 attctgtatg ggtatgaata agatctagtt ctagttcttt ggcaaccaaa taagcatacc
     3421 acatgcctct aacaacaact cttcgatcct tgaaagaaat aaatggtaca cttggcattc
     3481 gaatcacatc tttttccaac ttatccgcat taggatcggt cgttgtaaaa atatagactt
     3541 catgcccatg tctttctaat tcttgtttta atgttttgat cgatgtagca actccgctga
     3601 cttgcggaaa gtaggtatcg gtaaaaaagc cgattttcat gtttttcgcc tccaaactag
     3661 tccttgacct gatttacttt ttttaatttg ctagagactt tttcattcag tacactttcg
     3721 tatacatttt taagttcttc accgatttta ttgatacttc gctcttttgc tgtttgataa
     3781 ccggcttgtg tcgtactcgg tagttcttga ttggtaatct gttggatcag tcggataaat
     3841 tcttcgtttg tcttccccat atagcagttc ttgctatctt ctaaccagcc attatacact
     3901 ggaatatctc gaacaagtac gttttgtttg cttgctaaag cttctaaaac aacgatccct
     3961 tctgtctctt cataagaagg gaaaaagaat agatctgcac ctgaatacgc accttctatg
     4021 atctctccac gaatgtaacc aggaaacagg acattttcag gatgatcttc ttttacaatt
     4081 ttccggatgt tccgaggaat cgagtacatg ggaacatgac caaaccaaat aaatgtgtat
     4141 tcgggcatct tcttagcaat ttctacgaaa tcaatgattc ctttacgttc gaaaaacaaa
     4201 ccgacacaaa tgattacttt ttgatccgga gacagtttga aatattctct gaacttttgt
     4261 tctttcattg gatcagggtg atatttctct aagtcgatcc cattggaaat agcttgaatc
     4321 ggcacacgga taccatagct ttccaacaac tgttttgaat aaggtgttgg cgtgatcaga
     4381 tagtccgctt tttggtacaa tcctaccaga tattttttta cgataggaga aagctggttc
     4441 gatccgataa aggaatttcg gaaatcttcc tctgtagaat gcgcatgata gacaactttt
     4501 tttccttctc ttctggcttt attgaccata ttatgactat tgattccgta tgtattgatg
     4561 tgcaggatgt cataatcttc tttttcatct agtgtataag agataccgac ttccgtcaga
     4621 gcacgttttt gatgatcaag cgcacgcccg attccagatt ttgcaagcat cttttcactt
     4681 tcaaaatata ataatacctt caccttacgt cccccttcac ttatatagta taacatagcc
     4741 tacaaactgt cactcaaact taagccctat ttttgtctat tcttactgac aaaaacgttt
     4801 tcttaaaaaa gaacaaaaaa actggcgtag ctcttttaca agccacacca gcttttgata
     4861 cgttgtttat tttacatatt catgaacaag ttcaacgact tcttccatca tatcgcattc
     4921 tttcaatgca cgatcagcaa gttcagccat tttcgctgtg tccaagcgtt tcattaaact
     4981 acgcgtttta agaatagatg tcgcactcat tgagaactca tctagaccca tacctactag
     5041 caatggaaca gctgtttgat ctccagccat ttctccacac atacctgccc attttccttc
     5101 agcgtgtgct gcatcaatta catttttgat caaacgtaaa attgatgggt tatatggttg
     5161 gtacaagtaa gaaacgcgtt cgttcatacg gtctgctgcc attgtgtatt gaatcaaatc
     5221 gttcgtacct acagagaaga aatctacttc ttttgcaaac ttgtctgcta agactgctgc
     5281 tgctggaatc tcgatcatga tgccgacttg gatcgtatca gatacttcct tgccttcact
     5341 tatcaatttt tgtttttctt cttcaaagat cgcttttgct gcgcggaatt ctttgagtgt
     5401 agctaccata gggaacatga tacgtaaatt accatgaaca gatgcacgaa gcaatgcacg
     5461 catttgtgta cggaacattt cgtcgccttg ttcagataaa ctgatacgca atgcacgata
     5521 tcctaagaat ggattcattt catgaggtaa ttgcagatat ggtagttctt tatctccacc
     5581 gatgtccatt gtacgaacaa ctacaggttt tccttccata ccttctaata cagctttgta
     5641 agcttcatat tggtcatctt ctgttgggaa gtctggagaa tccatataca ggaattcagt
     5701 acggtacaga ccaactgctt ctccaccatt attatggaca ccaactagat cttttggtgt
     5761 tccgatgttt gcagctaatt caaaatgctt gccgtcagct gtgacagttt ctgcattttt
     5821 taatttttcc cattctgctt tttgtgctgc gtaatctgca cctgcttttt caaattcagc
     5881 tttttgttca tctgtaggat cgatgattac atcgccttcg atcccgttga cagccaaaat
     5941 atcgccttct ttgactttag cagtaatttc ttttgtccca acgattgctg gaatttctaa
     6001 agaacgtgcc atgatagcag aatgtgaagt acgtccacca atgtctgtta caaacgcttt
     6061 tacaaaattg cgatctaatt gtgctgtatc acttggagtc aagtcatgtg caacaacgac
     6121 cacttcttcg ttgatcattg atggatttgg taaagtaacg cctaataagt gcgctaaaat
     6181 acgtttagca acgtcacgaa tatctgcagc acgctcttgc atataagcat tatcttccat
     6241 tgcttcgaac ataccgatat acatatccgt tacttctttt agcgctgatt cagcatttac
     6301 gctgttgtct ttaatatttt gcttgatttg acctaccatt tctggatctg aaagaaccat
     6361 caaatgggca tcaaatactt gcgcttctgc ttcacctaag ctttgcgctg ctttttcacg
     6421 aatttgctgc aattcttcag tagatttagc taatgcagca tccaaacgag cttcttcagc
     6481 cgcagtatct tctactgttg ccttactgaa tgttaaatcc ggttgaacta gcaggtaagc
     6541 ttttgcaacg gctacaccgt cactagcggc aatccctttt agcatttcaa ccattattca
     6601 gataagcctt ctttcttcat tgtttcaacg attgctgcca ttgcatcagc ttcgtcagca
     6661 ccttctgcag taatagtaac atcagaacct tgacctacac ctaaagacat aacgcccatg
     6721 attgatttca agtttactga tttaccttta tactctaagt ttacatcaga gttaaatttg
     6781 cttgctgttt gtactaatag tgtagctgga cgagcgtgga tcccagtttc tgctactacg
     6841 tgaaattctt tcttttccat attaatcggt ctcctttaag tgagtgttgt ttttatgtga
     6901 gggttttctt ttttagtaat tcagagaatc ctaacaaatg ccaccctttc atctgtaaag
     6961 attaacattt tttttcgtta gatacaacag ttttcattca atttcagata aaagtattca
     7021 aattattccg gattcttttt catccttttc ttcacatttt ataacaaacg tgtttatttc
     7081 ctttttcttg tcgctttctc ttctcctgac gtttatcatt ctttcctttg accctctttg
     7141 ttttcatatg aaacgaatta cttgcttccc tttttatttt tagtataata agagtcaaag
     7201 gtcaggaatg gtcaatagag atttcacctt ttctacctca taaatctctt aaaaaactag
     7261 gggttattct tttacctcat gaaaaacgag atgtaaaata tggatgtacc catttttgtg
     7321 aaaggacgtg aatgattcat gctttgtcaa aactgcggca agaatgaggc aacgattcac
     7381 ttgtatgcca ctgtcaatgg tcagcggaca caactcgatt actgtcagag ctgttatcaa
     7441 aaaatcaaaa atcaacaaaa tggaggtctg actaatatgg cccaaaatga tccatttgga
     7501 tttggcagtt tggacgatct tttccgttcc atgtctcgcc aaatgcaaca gcaaggaaat
     7561 tatgagcaaa caccaccaac acaatttggc ggaaacaaca acttcaatgg tggtcagcca
     7621 cctcaaggca atacagaagg tttattggga gaatacggaa tcaatatcac agaacaagca
     7681 cgacaaggtg atatcgatcc tgtgatcgga cgcgatgacg aaatcaaacg agtcattgaa
     7741 attttaaatc gccgaaccaa gaacaatcct gtcttgattg gagaaccagg tgttggtaaa
     7801 acagcagttg ttgaaggttt agcacaaaaa atcgtagatg gtgatgtacc gcaaaaatta
     7861 atggacaaag aagtcattcg tttagatgtc gtatctttag tccaaggtac tggtatacga
     7921 ggacaatttg aagaacgtat gcaaaaattg attgaagaga tccgtcaagc agaaaatgtg
     7981 atcctattca tagatgaagt tcacgaaatc gtcggtgctg gttctgctgg tgatggaaat
     8041 atggatgctg gtaatatttt aaaaccagct ttagctcgag gagaattgca aatggttggt
     8101 gctactacct taaatgagta ccgcatcatc gaaaaagacg ctgctttaga acgtcgtatg
     8161 caacctgtac gggtagatga accaactgtc gaagaaacga tcagtatttt gaaaggtttg
     8221 caaaaacgtt atgaagacta tcatcatgta aaatatactg atgaggccat cgaagcagcc
     8281 gcaactttat ccaatcgtta cgtccaagat cgcttcttgc cggataaagc catcgatttg
     8341 ctagatgaaa caggttctaa gaaaaacttg acgatccaaa tcgttgatcc taaaacaatc
     8401 gagaaaaaac tgcaagaagc agaagaacaa aaagttttag cttctcgaga agaagatttc
     8461 gaaaaagcag cttattatcg cgatcagatc aataaacttc aaaaaatgaa agaacgtcag
     8521 ctcactgaag aggaaacacc tgtgattacc gaaaaagata tggaaaaaat cgttgaacaa
     8581 cggacaggta ttccagtggg tgaattaaaa gaaaaagaac agacacaact gaaaaactta
     8641 gcagatgact tgaaagctca tgtcatcgga caagataacg ctgtagatcg agtagcgaaa
     8701 gcgattcgcc gaaaccgtgt tggattaaac aaacagaatc gtccaatcgg ctccttctta
     8761 tttgttggac caactggtgt aggtaaaaca gagcttgcga aacaacttgc ctacgaacta
     8821 ttcggttcac aagattcaat gattcgtttc gatatgtctg aatatatgga aaaacatagt
     8881 gtctctaaat tgattggttc tcctccaggt tatgtcggat acgaagaagc tggtcaatta
     8941 acagaaaaag tacggagaaa tccttacagt cttgtcttgt tagatgaagt agaaaaagct
     9001 catccagatg ttttgcatat gttcttgcaa atcttagatg atggtcgatt gacagatgca
     9061 caaggaagaa cggtcagctt taaagatacg atcatcatta tgacaagtaa tgccggaact
     9121 ggtaaagtag aagcaaacgt tggttttggt gcggcacgcg aaggtgttac tcgttctgta
     9181 ttgaaccaat taaacaacta ctttacacca gaatttctga accgttttga cgggatcatc
     9241 gaattcagtg cattatcaaa agaaaacttg atgacaatcg tcacactaat gctagacgat
     9301 gtcaaccaaa tgctggcagc acagcaactt cacatcgaag ttcctacgaa tgtcaaagaa
     9361 aaattagttg acttaggcta tgatccatct atgggtgctc gtcctcttcg acgcacgatc
     9421 caagaacaaa tcgaagatgg tatcgctgaa ttttatcttg atcacccttc tatccatgag
     9481 ttgaaagcaa aactggataa agatggaaaa atcatcgtta cttctaaacc agaacgtttg
     9541 gctaaagaat cagccgaaga aacggctgaa taaatccagt tttatagcgt gagacggtat
     9601 ttctttgaga aatgccgtct cattcttttt tcagaaaagt caaaatactg ttcattttat
     9661 aaaaaagaaa ttataataag agaaacaagc aattaaaaat acataaaaga ggtgagcgcc
     9721 atgaaattgg taaatgtaac aaacagccat tcccgtcttg tattaaacca gcttgaaaac
     9781 actgatgccc atctagtcaa ggtctacaca gccggaaata ccactattgt ctacactgaa
     9841 gcccctgaac ataatgaaat tctgctgatc aatgacaaac gtaaaatcca gccgaaagaa
     9901 atcgaagatg ctaaaaatta tttcttagat aaaatcaaag acgccgttta ccacgaggat
     9961 gaaatcaaag taatcgaatt ggcaggcctt gttgaaatct cgattccaaa acatgaatta
    10021 ggcgctacat tagccgtgta aacaaatttt gaacataata attaaagagc gtgggacaaa
    10081 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcgaaag cagaagcaac
    10141 tccttcggaa ataagctgaa attcacgaaa attgcttttc aaatttttgt gaattccagc
    10201 ttatttcttg gagttaaacg cttctgtctc agcctctttt tctttctagc tttttgattt
    10261 ttctcttttc ttttgtactt ttctcttttc attttcagtt ctacgcgtgt ctttattttt
    10321 aactttttcc tctttatgga tgattgatcg tactttttgt gcaatttgct cgtttttgtc
    10381 ccagtctttt acatctaata atcgacataa tcgtaacatc tcttcttttt tctgtttttt
    10441 tctttcacgt ctttgccatt ctgtttccat gtgtcacact ctcctttctt taatcatatg
    10501 tacgcaaaat aatttttata tttttttgat tagacatttc tatgatttta atatagtaaa
    10561 aagagccgtc acggaaaatc cgtcacggct ctaaacaaaa tatgagaggc tgtttttgtt
    10621 tggctattct tattacttga tgcagggcag cttgtgtcaa gacttcttta catcaaactt
    10681 tttaatttta cattgggata tttatccgca aaccagcgtt ctgcaaattg gttctcgaat
    10741 aagaacaacg gttggtcgaa acggtcacga gccaagatat tgcgacttga actcatcttc
    10801 tcatccaaat cttctggatc gatccagcga gcgattttgt gtcccattgg agacatgatt
    10861 acttcggcgt tatactcatt taacatacga tattggaata cttcgaattg tagctgacca
    10921 acagctccaa tgatatattc ttccgttaga tatgttttat aaagctgaat cgctccttct
    10981 tgaactaatt gatagattcc tttgtggaag gatttttgtt tcatgacatt ttttgcagtc
    11041 actttcataa acagctctgg agtaaatgaa gggagttctt cataagcaac ttttagtttt
    11101 ccttcgaaca atgtatcccc aatctgataa ttgcctgtat catatacacc gatgatatcg
    11161 cctgctactg ctttttcgac gttttctctc gaatctgcca taaattgtgt gacattgctt
    11221 aatttcagtt ttttatttgt acgttccaac cagacatcca ttcctcgttc gaatgttcca
    11281 gaacagattc ggacaaaagc gattcggtca cgatgggctg gattcatatt tgcttggatc
    11341 ttgaatacga aacctgaaaa ttcttcctca taaggactta ccatctgatc atcttgtgtt
    11401 ttgtgtccat aaggagctgg tgccaattca acgaaagtct ctaaaaaagt ctgtacaccg
    11461 aagttagtca atgcagaacc aaaaaatact ggtgtctgat cgccacgagc aattttttct
    11521 tgattaaatg catctccagc ctctttgatt agttctactt cttctaagac ttgttcatat
    11581 aaggaatttt cagttagagg atgattttcg ggaagcagtc catcttcatc caattctact
    11641 aatcgttcat cctgataatt ttccggacga taaaactcta cacgattatg gtagatatca
    11701 taaagacctt ctagcccttt acccataccg attggccaat tcatcggata agattcgata
    11761 tctaatagct cttctaattc ttctaacaat tccaatggtt cacgaccatc acgatctagt
    11821 ttgttgataa aagtaaatat cgggatgcca cgttttttta caacttggaa taatttcttt
    11881 gtctgtgctt cgatcccttt tgcactatcg attaccatga ccgcactatc cacggccatc
    11941 aaagtacgat aagtatcttc agaaaaatct tcatgtcctg gtgtatccaa gatatttact
    12001 cgtttccctt gataatcaaa ctgcataaca gaactagtaa cagaaatccc acgttgtttc
    12061 tcgatttcca tccagtctga tttggcaaaa ttacctgttt ttttgccttt tacggtcccg
    12121 gcttgacgga tagccccacc aaacaatagt aattgttctg taatcgttgt tttcccagca
    12181 tccggatggg aaatgatcgc aaaagttctg cgattgtcca cttgttgctt taattttgga
    12241 ttattcataa cctaactcca tttcttccat tcaattattt atattttctc taggcacaga
    12301 ttgtccgatc tcttaaagaa agacaatttc atatagggct gtgacaaaag ccgtgtagca
    12361 gtctcttttt ccgagtaaac ggcgttctag agacagccta tcagcaataa gcccacccaa
    12421 gccaaaaata cgaaaagctg tttttgcttg gctgctctta ttacttaatg caggatagct
    12481 ttttcacaac ctttgtcaac tactacaact gaatcagtat agcatggatt cagcgaatga
    12541 aaaagccacc aaaccataag agtcaaatat ttttttgtca gctgactaaa agaaaagacg
    12601 tgttgcttgt ttattcaagc aacacgtctt tcataaccaa gtttccaatt atcttcttgg
    12661 ctcgtcatct tcgaattctt ttctgaagta tcttcgttct tcttcaggtt ccgcttctgt
    12721 ttcttccgga tcatgaaaaa tgacacggat tttaaggatt cgagaacctt ccatttcttc
    12781 agaaaccagc gtaatattat cgacatcaaa cgataacttt tcgccttcat ccggaatcat
    12841 gcctaatgct gtaatcaaat aaccagccat cgtatctaca tcactcatat gcagatcact
    12901 gtcaaaagct tcattgaact catcaatcag catacgtcct tggatgatat attcgtgttc
    12961 atcgattttt tcataaagat tctcaacttc atctgattca tcatcgattt ctccgacgat
    13021 ttcttctaaa aggtcttcta gtgttgctaa cccaacgacg ccgccatatt catctaataa
    13081 aatagccatc tgattctgcg tctttttcat ttcatataac agatcatcaa taaagaccgt
    13141 ttcaggaacg aataaaggct cctgcatgat tttcttgata tctaaattat caaagccgaa
    13201 tttatgggca gcttttagta aattctttgt atgaaggatt ccgactactt tatctttgtc
    13261 ttcgttataa acagggattc ttgaatagtt ttcggataaa acttcattca cattttcttc
    13321 cacagtgtcg ttgatatcaa tcataaatgc atccgttcgt gggaccatga cttctcgggc
    13381 aactttcgta tcaagagaaa aaaccccttg gagcatttct aattcttcat tattcaatac
    13441 gccttcgttt tcaagcatat aacgcatttc atctctggtc atttttgaat cttcatcatc
    13501 aaaagtcatc ggcgtgatac gcgccaataa acttgtggaa gcagaaagca gccagacgaa
    13561 gggcttagct actacaccaa tcaagcgtat cagccctgaa gtgaactttg cgacttcttc
    13621 tgatttattt aaagcaatac gtttagggta caattctcca aaaacaatcg atacataggt
    13681 caaaatagcc agcacaatga tatttgcaat attttttgcc gctgtgccgc cacctaatac
    13741 cggagctaac cgttccgata aagtattagc tagtgaagca ccagacaaaa tgttgaccaa
    13801 agtgattcct acttggattg tcgataggaa attatttgga ttctgcaata tttttaaaag
    13861 cttttgagat ttcacatctc cttcatcagc tttttgctcg attcgattct tattgatcga
    13921 aacaacagca atttcggaag ctgctaaaaa agcattgatc aatgtcaaaa cgatcaataa
    13981 taaaatctgc gctaacagcg actgactctc agggtcagca ttcataatcg cattctctcc
    14041 ttataaaatt aatttttagt ttacgataga aaaaaacgtg gcactatagc cacgttaaaa
    14101 tggtatcaca aagcaatttt ctttgcaata aaaacaagtg tactaaaact acctacgagg
    14161 tcgtagcaat tttcttcttg ttttcatttt gcttgtcttc tttgacgatt ttgattatat
    14221 aggaaatgat cgtcttgcag attgcgtaaa aaggaacacc taagaagata ccaagcaatc
    14281 cggcaatatt tccagctact agaagaacga taatgatcgt taatggatgg attttcagtg
    14341 atttcccaat tacattagga taaatgatat tcccatccaa ctgttggacg attagcacaa
    14401 ccacacaaca aagtaatgct tttaccggat cattgaagac tgttactaga aatgcaggtg
    14461 ctagtcctag ataaggtcct aaatatggaa ttaggtttgt caatcccgca atgaccccaa
    14521 ataagaaagc ataatccaca ccaattacca tatatcctaa aaatgtgaag gtaccaacga
    14581 ataaacattc gattgcttgt ccgctaatgt aattagccaa tgttttattc agttgcccca
    14641 ataactccac aatttggttt cttctttttt ctgggaaaaa ctgttggatg tttggcacta
    14701 gtttttctcc gtctttcaac atatagaaca agatgaatgg tgctgtaatg attactacgg
    14761 ttgccgaagc gatcgttcca acgatcgaac ctaaactatt ggatactcca ctaaggaatt
    14821 gctgaatgat tgtcccgtaa gaaatgtcta attgctcaaa atatttattg atatctactt
    14881 ccttgaacaa aggcaattcg gctacctctt ttaaccagct ttctacctgt ttgatgaaat
    14941 caggcatatt agatgccaat gaggaaattt gctgtactag acttgggatg acactaagta
    15001 aaatccaaat aattgctgca actaaaagga gcagcacaat tgcgacagcc catattcttt
    15061 tgatttttgc tttcatcaac agcttgacaa taggattcaa taaatagtaa agaaagcctg
    15121 caatcagtac aggtgcaaat aacgtggaaa aaaatgtccc gatcggttcg aaaataaagt
    15181 ttatttctga tgaaatcatg atcaatgcag ctatgatcaa taattcaagc gaccaaaaca
    15241 ttagtttaga ctgtctaagt ttttcaaaca atagatttcc tcctttcttt cttactagta
    15301 tatattatat tcgttaaaaa ttcttttatt ctttttttat ttttgaaaac ttgccatact
    15361 ttttatttgt tttattttcc agtatactat acgaaaggaa ggtggctaga ccatgaaagt
    15421 tgtacaaaca aaagatacaa tgagcaatat ttacttagat gctgtacgga tcaggcatca
    15481 agtttttgta gtcgaacagg gtgtgccttt gtctcgagaa atagacaaag atgaagccca
    15541 ttgtatccac ttcgtcctgt actcagataa aaaagaaccg caaggaactg tacgtctttt
    15601 gcctttagaa aacggaaaaa tgaaattaca gcggatggcg attctttctg aatacagaca
    15661 ccaagggtta ggaaaaatat tgattgaaga agcagaaaac tttgctaaga atcaaggcta
    15721 taatacgatt ttattagggg cacagtccac agctgaaact ttttacgaaa aactaggata
    15781 tacagcttac ggcgatcctt ttgaagatgc tggcatgcct catatttata tgaaaaagac
    15841 tttataaaaa tttatttcga aaaaagcaaa aaacagcaag cttttgagaa aaatctctca
    15901 ctggcttact gttctttttt gtgtttactt acgcgacaat tgcttgtaac ggttatacca
    15961 aatatcaata tagccttccg agaacgggcc atctccatta ttgatccagt caaccagaat
    16021 cttcacattt tcttttagga tgaagtcaat atcgttcggg taattcatca ttttgctgtg
    16081 catctcatat tcatcgacat ccaatagtcg tttctcgccg tcaggaaata ccttgatatc
    16141 caagtcatag tcgatatatt tcaaggcctc atcgtccagc aaaaaaggtg aagctagatt
    16201 acaataatag gaaaccccct tttctctgat cattgctatt acattgaacc agtatttttt
    16261 atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg cgtccatcag attcagtgac
    16321 taatgtgtga tcattcacgc cgatcataga gtgttcgctt gtttttaata ccatggtatc
    16381 tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt tggatcgtca caaactctcc
    16441 ttcttttgga actcccatag caacccttcc ttaagcagca ttttgtgctt tgtgtttggg
    16501 tgttcattac accgacatca taattattat atcaataaaa atgagctttg cctagaaagg
    16561 attcctattg tttcgatttt tctctaactg atctaccagt ttcatttgga ccttaggaaa
    16621 aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa gttttgttct cggtaaattc
    16681 ttgttctgct ccctcggttg cccgtccgta aaaaaggaga acatgccatt tcagatgact
    16741 aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa acaaagagtt cctgcttttc
    16801 aaatgggaga ttttgctctt cttctgctac taggtcatca aacagatcga tttctggctt
    16861 tgtttcccat tcttttttca atcgctcata ctcctcttgt gtcacttcca tcattggaaa
    16921 tgtccacata ttcgccagca atttttggct atctctttct tcaaaataat acgctccaga
    16981 atgattttga agagctgcac tgatataata aacgtctttt ggctttgctt tttttgtttt
    17041 tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa caaaaggctt ggatcggaca
    17101 agtctcacac tttggagaag ttggtgtaca gatagctgaa cctagatcca tcattgcttg
    17161 gttgaattca cctggatgct tttcatcaat gattttccgc atcgcttcat caaagatttt
    17221 cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac aatctgctga ctactcgcat
    17281 cacgttgccg tcaactgcag gttctggaag tccaaatgcg atacttgcaa tcgcgcctgt
    17341 cgtatatggt cctattccct tcaatgagct aatttcttca ggcgtttgcg gcatttcccc
    17401 atcaaattct gacatgatct gtttagcagc agcttggatg ttccgggctc ttgagtaata
    17461 accaaggccc tcccatgctt ttaaaagctt ttcttctgga gcatttgcta gctcttcaat
    17521 tgtaggaaac cattccatga aacgataaaa ataatcgatc actgtgtcta ctctggtttg
    17581 ctgaagcatg atctcagaga tccagatacg ataaggatca cgattatagc gccaaggcag
    17641 gtttcttttt tcttgttcat accattgaat gaactgatct tgaaattctt ttgtttcttt
    17701 atctgtccat ttttcccatt ctttattcac tttcctcttc cttttccgta atgaatcgct
    17761 ggatcatatc caaatcaaat cctttttgat ataagctttg ctttactttt agatttcttt
    17821 ttttaggttc aaaccttaaa tttcttcgcc atagtttatc tccttgtttg actaaataat
    17881 catattctat ttcttgatct gcttcttgcg gaagatcagc tatcgcttgt tggatcactt
    17941 cttgagaaaa gcctttcgtc atcaatgttt gttggatttt tctcaataat tctttttggc
    18001 tttttccgtg attttttcca aacgcttttt cggctgtctg atgtgcattt tgaacttggt
    18061 cttcaaatgt atattggctg agcgcttgtt caatgatttc aggtttcaaa cctttttgct
    18121 gcatctgctg cgctaatttc tttggtcctt tgtcacttaa gcgcatattc gtgcgcacat
    18181 aactttcccc atagaccaaa tcatcgacaa ggtccaattc ttttaagcgg gctacgattt
    18241 tatttcgatc ttcttgaagg atttcctttt cttttaagta tgttcggatt tctttttccg
    18301 atcgcaactg ataacttaag taattcattg ctaggtgaaa accgaaatca gctccgctac
    18361 tcttcttgat ctcttggata gtttcttcgt ctaattcccg tccttttaat aatcgatagc
    18421 gtactaatac atcttcagat acttgtaaag cttctccttc agataaatct acgcgataga
    18481 aagggccttt gcctttactg attctcgtaa cggttaacat aaacatcctc ctcgaacata
    18541 tattctctat cataccacag aaaatccccg taatcaggta ttatatgtta gaataaacca
    18601 cagaaaaaag aaaaagggag atacttatgg cagaaacaat tgtcaaaact ggacaaacgg
    18661 ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg aatcggttat ttcaaacggt
    18721 tgatcatatt tgttccatat gcactaccaa aagaagaagt actagtgaaa atcacgaaag
    18781 caactcctcg ctatgcagaa ggagaattaa taaaagtcaa aaaaacaagc aaagatcgcg
    18841 tagtggcacc atgtcctgtt tactatgaat gcggtggctg tcaattacaa cacttagctt
    18901 atcaagctca attggatttt aaaaaggatc tgcttttgca agcattggaa aaattcaaac
    18961 cagcaggctt tcgatcttat caacttttgc cgacgatcgg gatggatgag ccttggcatt
    19021 atagaaacaa agcacaattt cagttacgaa aaaataagca gactcaaaaa attgaagctg
    19081 gtttatacga agcaaactct catcagttag tccctataac agactgcttg gttcaagaac
    19141 ctcgaacaca aaaagtaatg aatactgttg ttcaactatt gaataaattt gatgcaccca
    19201 tctatgatga acggacaaac agtggaatct tccgcacaat catggttaga atcggtgtta
    19261 aaactgggga gttacaagtt gtttttatta ctcgcagtaa aaaattcccg caaaaaaacg
    19321 caatgatccg tgagatcaat gaacgactgc ccgaagttgt ttcaatcatg caaaatgtcc
    19381 aggcacaaaa aacgtctctc gtgatggggg atgaaacgat ccacttatgg ggaaaagaag
    19441 ccattgaaga acaaatcaat gaagtgactt tcgatctttc tcctcgtgcc ttctttcaac
    19501 tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat caaagcatta gatgtccagc
    19561 ctaatgaaac gatcgtggat gcttattgcg gagttgggac gatcggctta agtgtagcaa
    19621 aacaagccaa agaagtacgg ggaatggata ccgttcctca agcaatcgct gatgcaaaaa
    19681 aaaatgcgga aagattagga tatcacaaca ctcgatatga agtcgggaca gcagagcaag
    19741 ttctgcctaa atggttgaat gaaggttttc agcctgatgg tatcatcgta gatccaccac
    19801 gtacgggttt ggataacgca ttgatccaat cattattgaa ataccctcca aaaaaacttg
    19861 tttatatctc ctgtaatgtt tctacacttg ccagagatct ggttaattta agtaaaaagt
    19921 tcaagattga atatttacag tccgtcgaca tgtttcctca gactgcacgt tgtgaagttg
    19981 tcgtgaagat gacgagactc gctgactaaa tgaaagaggt tgtgaaaaag tctgccctgt
    20041 atcaagtaat aagaacagcc aaacaaaaat agctcttcat gttttatgaa tactgtactt
    20101 gtgaccgaag agttaacagg aggacactat ttatttaaaa aaagggttgt gacatagttt
    20161 ttgtcacagc cctttttgat tattattgga tattcacgtc tatcacttga taaaatgcat
    20221 ttgtcgtatc gctgatattc catactgcta aaatcacatt atatcctttt cgatcctgcg
    20281 gaagtgtgat cgtttggttg actgtttttg gtggaacagt tgctttgtcg tctatttttg
    20341 tgatcaattc aaaatcacta aatttcaaag gttgattagg attccaagtt ggtttggtaa
    20401 tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa attccaagta atatttaatg
    20461 ttcctggatt aaccatgttt ttatgccatc tgttagatgt ctgttcatct aaaggctcaa
    20521 agccgctgac tcctgcacta gcaatttttc catcaatgaa tgtatttttg gtagcttcga
    20581 tactttgcgg ttcccattgt gctcttccga cattttgatt taagtttcct gctgctgatg
    20641 ttcctaggta tgcacggctt cctggagaag taatataacc atgcgcaaaa cctggaatgc
    20701 taaaaccgaa caaccctaaa ctaactaacc caataaacaa taacgtacta atctttttca
    20761 tgtagaaacc tccttcattt gataaccaca ttgtaaacgc tttattgtat taacacaaac
    20821 attatgcttt tataatttta tgttgaagta aaaaaggtga aaaaagtaag atgcagatta
    20881 taacagaagt agacgagaaa agtacaaata gaaaacatga gaatcaaatc atatgatctt
    20941 tttcaaaaac atcattcata taaacaaatg ttcaactttt taataacaac aattatcaat
    21001 ttttttctta cttcacaaac taaatacagg tcttgctgtg agccttcatg gtaaactaat
    21061 attatagaaa ggagttagta aaatgaagat gataaaagat attcgttttt gggtttgtgt
    21121 tataggaatt gtcatattag gctttttatc agggttgctt agcggaaatc ccggaaaata
    21181 ttactattct ttgcaacttc caccatttgc accacctagc tggatatttg ggccgatgtg
    21241 gaccttactg tatatattga tgggtatttc tctttatttg ttacttaatc acaacaacaa
    21301 aaaacagcga aacaatcttg tcggtctttt tgtgattcag ttcattttta attttatatg
    21361 gtctgctcta ttttttaatt taagaaatat tttcattgca gcaatcgata ttacactttt
    21421 agttattttc ttgtcagtgc ttatgtatca gttgtggctg catcatcgcc tggcaatgtg
    21481 gctcatgatt ccttattatt tatgggtact attcgcaact ctattgaatt attcaattta
    21541 ttttttaaac taagcgctaa aaagagacgt cctgtaattt aacacataga aaaagtgcca
    21601 cctaagttag attttttgtc taatttaggt ggcactcttt attaaccaaa ctattttttt
    21661 ctagtaccca cttgttgttc catactcatt attctggtca taaccataac cattttgttc
    21721 ctgtccagcg taaggatcat aactttgagt atcttgactt ggtgtatagt actgacttgt
    21781 atcttctgtt tgctgataat aggtattcgg atcttggtac tgatcatatg tgttttctga
    21841 ataggaagaa taatcggtgt acccttcact attcgatcca tcaccgtttg tcgaatcgcc
    21901 atcttccgaa tcatcaaaga acatcattga agcattctca tttttcaaat taggtaatgt
    21961 atcgcttgtt tttatattta attgtttctt cagttcattt tggatcgaaa gaagatcatt
    22021 ttttcctaaa atttgatagt agatatcatt gatcatttgt ccttcgcctt gcaactggtc
    22081 ttgtttgatt gtttcaaacg ccggggtgta gttcgtcgct acatcaagca tgtcatccca
    22141 atctaagtcc gtcgtcacat tgttctcaac tgcttttaaa atttttctat aattgcttac
    22201 gccatctaaa cttaaaactt tattgacgat tttcgtcact acttcccgct gacgacgttg
    22261 tcgaccgata tcgccttctg gatcttcgtg tcgcattcga gaataggcta aacctttttc
    22321 tccattcaat gtttgttcac ctactggtac atggacacca tccaaagtaa agtcaatttt
    22381 attattcact ttgatgccac cgaccgcatt gatcaaatct tccatgccat ccaaattgat
    22441 cgttacataa tgatcaatag gaatatcgag cagttgctca attgaatcca tggccatttc
    22501 gacaccacca aatgcgtagg catgatttaa tttatctacc gttccataac ctacgatatt
    22561 cgtataaata tctcggtcca aactgatgat cgttgatttt ttttgtttcg gattaactgt
    22621 tactaccatc atcgtatcag aacgtccttg ttccgttctt cctaatccac cggtatctaa
    22681 acctaatagc aaaaccgaaa aaggttcttt gtcatcaatg cttactctct cagaacgttt
    22741 ggaggatatt cgattagagt ttttagaaat tttgtttact gtttggctcg cttctcccat
    22801 aaatctgatt ccgtatgctg ataatccggc aatcagcaaa acgaataccc ctaacaaggt
    22861 taatagaact ttttgccatc gcttcataat acacctctgt ttcacatttt ctaaaatatt
    22921 gtctcatata actgttcaat cagcaaattc actttttaat cataaga
//
LOCUS       DACESX010000037        22789 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.037, whole genome shotgun sequence.
ACCESSION   DACESX010000037 DACESX010000000
VERSION     DACESX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.037"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            76..1269
                     /locus_tag="IXF88_001200"
     CDS             76..1269
                     /locus_tag="IXF88_001200"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1507940.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1399..2871
                     /locus_tag="IXF88_001201"
     CDS             1399..2871
                     /locus_tag="IXF88_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1507941.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            2954..3742
                     /locus_tag="IXF88_001202"
     CDS             2954..3742
                     /locus_tag="IXF88_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1507942.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3809..4477)
                     /locus_tag="IXF88_001203"
     CDS             complement(3809..4477)
                     /locus_tag="IXF88_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1507943.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGIKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4592..6304
                     /locus_tag="IXF88_001204"
     CDS             4592..6304
                     /locus_tag="IXF88_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507944.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6307..8076
                     /locus_tag="IXF88_001205"
     CDS             6307..8076
                     /locus_tag="IXF88_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507945.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8281..8847)
                     /locus_tag="IXF88_001206"
     CDS             complement(8281..8847)
                     /locus_tag="IXF88_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1507946.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(8847..9803)
                     /locus_tag="IXF88_001207"
     CDS             complement(8847..9803)
                     /locus_tag="IXF88_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1507947.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(9803..10459)
                     /locus_tag="IXF88_001208"
     CDS             complement(9803..10459)
                     /locus_tag="IXF88_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1507948.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            10829..12847
                     /locus_tag="IXF88_001209"
     CDS             10829..12847
                     /locus_tag="IXF88_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1507949.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFSARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            13274..15688
                     /locus_tag="IXF88_001210"
     CDS             13274..15688
                     /locus_tag="IXF88_001210"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1507950.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16036..17589
                     /locus_tag="IXF88_001211"
     CDS             16036..17589
                     /locus_tag="IXF88_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1507951.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(17970..19754)
                     /locus_tag="IXF88_001212"
     CDS             complement(17970..19754)
                     /locus_tag="IXF88_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507952.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(19769..21490)
                     /locus_tag="IXF88_001213"
     CDS             complement(19769..21490)
                     /locus_tag="IXF88_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1507953.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            21590..22351
                     /locus_tag="IXF88_001214"
     CDS             21590..22351
                     /locus_tag="IXF88_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1507954.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7256 a         4078 c         4711 g         6744 t
ORIGIN      
        1 tcgttgcaag ttcccgaaag gatccgcagg acggagatgc cttgtaaccg aatttaacta
       61 gggggaatca ttataatggt agaaagacac ttatttacat cagaatctgt ttctgaagga
      121 catccagata aaattgctga ccaaatcagt gatgcaatct tggatgcaat tttaaaacaa
      181 gatccaacag cacgagtagc gtgtgaaacg tctgtaacaa ctggtcttgt tttagtattt
      241 ggagaaatat ctacgacggc atatgtagat attcaaaaag tcgtacggga aacaataaaa
      301 gaaattggtt atacacgtgc aaaattcgga tttgatggag atacagcagc tgtattggtt
      361 gcgattgatg aacaatctcc tgatattgct caaggagttg atgaagcact tgagatccgg
      421 gatgaagata aaaaagacgt attagatgag attggtgctg gtgaccaagg tttaatgttt
      481 gggtttgctg tagatgaaac accagaattg atgcctttac caatcgcttt gagtcatcgt
      541 ctggtacgac gcttggcaga tttgcgtaaa tcaaatgaat taacgtattt acgtccagat
      601 gcaaaatctc aagtaacggt tgaatatgat gatcaaggac aaccggaacg cgtagataca
      661 atcgttattt caacacagca tgatgatgca gtagataatg aaacaattcg tcatgatgtc
      721 atcgaaaaag tagtgaagga agttattcca gctgaattat tagatgatca aacgaaatat
      781 tatatcaatc cgactggccg atttgtcatt ggtggtcctc aaggggatgc cggattaaca
      841 ggaagaaaaa tcattgttga tacgtatggc ggttatgctc gtcatggtgg cggtgctttt
      901 tctggtaaag atgcgacaaa agttgaccgt tctgccagct atgctgcacg ttatattgct
      961 aaaaacattg ttgcagcagg gcttgctcgc aaagcagaag tacagctagc ttatgcgatt
     1021 ggtgttgctc aacctgtttc gatctcaatc aatactttcg gtactggaac ggttccggaa
     1081 gaagaactga ttgctgcagt gagagaaaac ttcgatcttc ggccagcagg aatcatcgag
     1141 atgcttgatt tgcgtcgtcc gatttacaaa caaacagctg cttacggtca ttttggtcgt
     1201 acagatgtag atttgccttg ggaacatacg gacaaagtag atgcgttgaa agcaagttta
     1261 gcaaaataag ttatattcag ctggaacgat cgccaagtgg tggttgttcc agcttttttt
     1321 atggcagtta acctcttgaa aaagatagta attatttatc tatttttaga tcctgtcata
     1381 atgaaaggga ggaataagat gaaaaaagaa acaaatgtta ctttggtcac agtgagtatt
     1441 ttcattgcaa cgttcatgac ggctattgaa ggtacaatcg taacaaccgc tatgccaacg
     1501 attgtcggtt cattacatgg aatggaaatc atgaactggg tattttcgat ttatttgctg
     1561 acaaacgcta tgcttactcc gatttacgga aaacttgcgg ataaagtggg acggaaacca
     1621 atctttattt ttgggacatt attgtttatt atcggttcgt tattttgcgg tttatctcaa
     1681 agcatgattg ggttgattgc tgctcgtgcg attcaaggga tgggtgctgg ggcaatgatg
     1741 ccagtagctt tgacgatcat cggcgatctt tattctattg aaaagcgagc aaaagtttta
     1801 ggtttgaata gttcagcctg ggggattgcc agtgtttttg gaccgcttgc gggaggattc
     1861 atcgttgaaa cgatcagctg gcactggatc ttttttatca atgtgccgat cggaattcta
     1921 ttaattattt tgatttggct atatttaaat gaaccaaaaa tcatacatga gtctaaacca
     1981 atggatattt ttggtagttt tagtttgatg gccatgcttg tctcattgct gttagggttc
     2041 caattattag gagacgaagg aatgagtccg cttgtttctg ctttgctgct aggtagtatc
     2101 gtaatgttag gtatttttgt ttttgccgaa aaaagagcta aagatcctgt catcatgatg
     2161 catttattca aaaatccact atttgttgtg gtcaacttgg ctgcagcatt ggctagtggt
     2221 tttttgatgg gaatcgatgt ttatatccct atgtggatgc aaggtgtatt agggctgaat
     2281 gcagcaattg gtgggctggt attagcacca ttatcactag tctggatggt cggttctttc
     2341 cttgcaagca agtggatgac tcaaatgaaa actcattggg tcttgagaat tggactacta
     2401 atcgcagcta ttgggggcgc ttggttgtat cttatgcctt tcagaagtaa ctatttatgg
     2461 ttttttgcta tatcaacggt catcggtata ggcttaggtg tgaccatgac tacctcgact
     2521 gtcagtgcac aaaatagtgt atctcgtgac cagcttggtg tagcaacttc ttttaatacg
     2581 ttagttcgaa caattggtca aacagtcatg gtttctattt ttggtttatt gataaatggg
     2641 gtaactaaac gagaacttgc agcagcaaat ttaacaaatg atgcagatat catgaataaa
     2701 ctggtgaatc cacaaacggc taaatatatt gatgcggata ttttgttacc attaagaaaa
     2761 attctttatg atgggttaca ttccgtttat ctagttggct tgttactcgt gatcattgct
     2821 ttggtcttaa cgcttttcgt tcatgaaaaa agtggtaaaa taaaacagta gtttcggtta
     2881 agatatgtac gtagccgaat cattcaaatt taaagttatt caaaaataac ttcttactaa
     2941 ggaaggcgaa aatatggcat ttattcaagc aaatatttat tcgaatgtgt tagaaatgga
     3001 agtcaatcta aatgtgatac taccgcaaac aacaaaaaag gtgattggca ccagttcaga
     3061 gcaagagaca acggatatac ctgttttata tttacttcat ggaatgggtg gcaatcatag
     3121 tgtatgggaa agacgtacgt cgattgaacg ttatgtagca aactacggat tagctgtcat
     3181 catgccatct actgatcttg gctggtacac agatacagtt tatgacatga agtattggac
     3241 attcattgca gaagagctac ctgagatttg tcatgaatta ttcccgcaat taacaagaaa
     3301 aagagaaaaa acatatgccg ctggattatc gatgggcgga tatggcgcgt taaaactagg
     3361 ccttgcaaaa tcagaaaatt tcgcagcagt aatctctctt tcaggcgctg tttctgtagg
     3421 aaatcgaatg gatgatcttt tgatggttcg taaagcatct ttttgggaag ggatctttgg
     3481 accattggat caaatcgaag gttcaaaaaa tgatccgcaa tacttattga aagaactagt
     3541 aaaaagcgga aaaccagttc caagatttta tatggcttgt ggggaatctg attttcttta
     3601 tcaggcaaat caggaaatgg tatctgctat gcaggaacaa ggcttggcgg tcacctttga
     3661 acaaggacct ggcgatcata actggatctt ctgggaccaa tggattcaac gtgcattggc
     3721 gtggctaatg gaagaaaaat aaaaaaagag tcttaaaaag atagctccta tagagatcac
     3781 aaacatagtg atctctatag gagctttttt actgctcgtt ttctcttctt ttattttgtt
     3841 ggcataagcc ttcaatatag gtttgtgcac tcgttagaaa gagtgctttt tcattcaatt
     3901 ctgcatggga ctgcatactt tctttaattc cctctaattg attttcaact acataattac
     3961 gcataaatag aactgtttct ttcaaaaatt catttccgtc cataattttt tgtcgaagct
     4021 gctgttcatg ctgctgatcc attaactgtc caatcaataa attgtggaga gaggcaatgg
     4081 cctgatgact ttgggtgact gaaaatcctt cacgaacaag taattggatc atttgctcca
     4141 aatgttctaa tcgactcgga aaagcgggca cagtattgat ttctaattct gccccacaag
     4201 ggaaacgtgt ataaaggtca tagtaatttt ccatatactg cagcaactgt tcattccaag
     4261 gcaaagttgg atctggaagc gctaattctt cttccattgt ttcagccatt ttttgcaaga
     4321 gctgttgttt gtttttgaaa taccaatata aagcaggtgc ttgaacatga attctgtttg
     4381 cgatgtttcg catggaaagc tgcgaaagag cttgtgtttc agccaggata tcaaaggcgg
     4441 cctgaatgat gattgcttga gaaagcttta tttccatttt atcaacctcg tgtattgcat
     4501 tatcttttta aacatggtaa attgttagtc ggtctaattt tacagtgtta aattagtcct
     4561 gtcaatttaa acaaaggggt gtaatcaacg aatgaaatta atcttacgac atgcgaaaaa
     4621 atataaagta gctgttttta tttccttgct gtccgtagca gtcatggttg cagcagcatt
     4681 atggcaacct aagttactac agcaagtatt ggaagctatt attacagaag ataatgatga
     4741 aatgaaaaca attggtattt acctgatcag tctggctttg ctaggattag ccgcaggtgt
     4801 aacgaacacg attttctctg caaaagtagc tcaaggcgta agtgcagata tccgagaaga
     4861 agcatttcga aagatccaga cattttcatt tggaaatatc gaacagttct ctgctggaaa
     4921 tcttgttgtt cgtctaacaa atgatatcac gcaaatccaa aatttagtga tgatttcttt
     4981 gcaatcactt tttcgaattc cattcttatt tattggtgcc ttcattttag ctatgatcac
     5041 gatgccgcaa ttatggtgga tcatcgtagc gcttatcgtt acggtatttc tgattacggc
     5101 actttcattt acaaggatgg gcaagcactt catgatcatt caaaaactga ttgatcgtgt
     5161 gaatagcatt gcaaaagaaa acttactagg gattcgtgta gtgaaatctt ttgtacaaga
     5221 agaaaaccaa ttgaagtcat tcagcaaagt aagtgaggac ttaacaaaac acaacatcat
     5281 agtcggtaca ttattttctg tgatgatccc gtccttcatg ctggctgcca atttggctgt
     5341 ggttggggcg atcttttttg tgagcgatct tgttaaagat gatcctacgt taattggtgg
     5401 aattgcttca ttcatgaact acttgatgca gattatgatg gcaatcatta ttggcggaat
     5461 gatgatgatg atgacttcaa gagcagccgt atcgctgaag cggattgcgg aaattttaga
     5521 tgctgaacca gatcttactt atttagacgt tccagaacaa gagttgacgg gttctgtcac
     5581 ttttgaccat gtttcttttc gttatcctgg tgaggataca gatacgctta aagatatcag
     5641 cttttctatc aaacctggag aaatggttgg tatcgtcggg gcgactggtg ccgggaagtc
     5701 aacactagca caattgatcc ctcgtctatt tgatccgaca gaaggcaaag ttgaagtagg
     5761 tggtgtagat cttcgtcaag taaatgaaaa gagtttaaga caaacggttt cctttgtttt
     5821 gcaaaaagca attttattct caggaacgat tgctcaaaac ttgcgccaag ggaaaaagaa
     5881 tgcaacagaa aaagagatgg aacatgcttc ttctattgct caagcaaaag aattcatcga
     5941 aaagttatct gatcgttacg aagcaccaat agaagaacgt agcagtaact tttctggtgg
     6001 tcaaaagcaa cgtttatcca ttactcgtgg cgttatcggt aatccaaaga tcttgatcct
     6061 agatgacagt acgagtgcac tggatgctcg ctctgaaaag ctggtccgag aagcactcga
     6121 tcgagatttg aagggaacaa cgacgattgt catcgcacaa aaaattgctt ctgttgtcaa
     6181 agcagaccgc atcttagtat tggatgaagg cagattagtc ggcgaaggaa cccatgagga
     6241 attggtagct ggaaaccgtg tctatcagga aatctttgaa actcaaaaag gaacggagga
     6301 gtaagtatga ctgatctaat aaaagcaagt aaatttttct atcactattt gaagcggtac
     6361 aaactctcct ttttctttat ctttatcaca gtgatcattg ctacctatct acaagtaaag
     6421 gcacctcaat acatcgggga agcttttcaa gaattagcta attacgtagg aggcttgatg
     6481 caaggcgtag acgataaaag caaatttgtt tctgtaatat ggaaactctt gctgttttat
     6541 gtactagcta gtgcagccaa ctttatctat agtattttgt tcacacaagt agtagggaag
     6601 tcaacgaacc gaatgcgtat cggtctattc aacaaattgg aaaaattgac gatccgtttc
     6661 tttgattctc atcaagacgg tgaaatttta agtcgtttta ccagtgactt agataatatt
     6721 caaaatagtt tgaaccaagc tttgcttcaa gtcatcacga atgctgtttt acttgtcggg
     6781 atcttgatca tgatgttccg ccaaaatgta gagttagctt gggctacaat tgcttccact
     6841 ccagtagccg ttttgatagc tgtcttgatt atccgcaaag cacggaaata tgtggatctt
     6901 cagcaagatg aagtggggaa actaaatggt tatatggatg aaaaaatcag cggacaacgt
     6961 gtcatcatca caaatggctt acaagaagaa acgattgaag gattcttaga gcataatcaa
     7021 agtgttcgcg aagcaacctt caaaggacaa gcttattctg gtctactatt tccgatgatg
     7081 caaggaatgt cactggtcaa tacagcgata gttatctttt tcggcggctg gttagcatta
     7141 aatggtgatt tggaacgttc ggtggcatta ggcttagttg tgacattcgt ccagtattca
     7201 caacaatatt accaaccact gatgcagatt tcatctggat acagcatgat ccaattagcg
     7261 attacagggg caagacgatt gaatgaaata tttgatgaaa aagatgaagt caaaccagaa
     7321 aacggtaaag catttgatgg tgttgaaaaa ggtcttgcgt tgcagcacgt agattttggt
     7381 tattctccag aaaaactagt cttgaaagat gtctcgatca atgtggacaa aggagaaatg
     7441 gttgctcttg ttggtccaac aggatcgggg aaaacgacga tcatgaatct gctaaaccgt
     7501 ttttacgatg tcaatgatgg ttcagtagtc atcgatggaa tagatatccg agattatgat
     7561 cttgatgcat tacgtagaca tgtaggaatc gttttgcaag aatctgtatt gttctccgga
     7621 acgatcaaag acaatatcac attcggcaaa ccggacgctt cagatgaaga agtcatcaat
     7681 gcagcaaaac aagcgaatat tcatgacttc atcatgacgt tagaaaaagg ttatgataca
     7741 gagatatccg aagaaaataa cttattcagt acaggtcaaa agcagttgat cagtattgct
     7801 cgaacgatca tcacaaatcc atctttattg attttggatg aagcaacaag taatgtagac
     7861 acggtaacag aagcaaggat ccaaaaagca atggatgaag caatcaaagg aagaaccagc
     7921 tttgtgattg ctcaccggct gaaaacaatc ttgaatgcag atagaatcgt tgtcttgcgt
     7981 gatggggaag tcattgaaga aggcagtcat cacgagttat tagcagaaaa tggattttat
     8041 gcggaacttt atcataatca atttgtattt gaataaagag gttgtgaaaa agctgtcctg
     8101 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgat ttggttggac
     8161 ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga aatcaacgct
     8221 ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg cgttttttat
     8281 ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt tgataaaccg
     8341 ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat caaccatatc
     8401 cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc gtccgcgagg
     8461 cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca tagtgatgat
     8521 tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga gattttgttg
     8581 ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga cacgttcttc
     8641 taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct ggatatcaaa
     8701 actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat cgtggccatt
     8761 gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca gtaatgtatg
     8821 gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac atcatatttt
     8881 ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac ttcccatttt
     8941 ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt caaacgatgg
     9001 atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac atagtcagga
     9061 cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt tttgcgcatc
     9121 agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt gcgtctgaca
     9181 ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg cgtacaaaca
     9241 cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata atcatgtgct
     9301 cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc tacccataaa
     9361 tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag acaatccggt
     9421 cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc aaatcgttct
     9481 ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac gatgtattgt
     9541 tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg gattggaaga
     9601 gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa agtacaaccg
     9661 ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat cggcaccttg
     9721 aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata tcttttattt
     9781 ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta tctatttgtt
     9841 ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg agtaagtagc
     9901 cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc ccgacactta
     9961 ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt tgaacaaaag
    10021 ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca tgtccactag
    10081 ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct cgcatgaaaa
    10141 atagtttgat caccgggttg atgattccag caattacaat aaatccagaa acgaaccaaa
    10201 atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca aataaaatga
    10261 tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc agatcaggat
    10321 aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac gacagatgga
    10381 actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca gccacctgaa
    10441 atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta tagcattttt
    10501 ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct ttgaataacc
    10561 aatattctta taaaaaaaat aactgtaatc ggaaagacat tttttggtaa tgcaaatgaa
    10621 atgttagtga aataaaatca gtgtttttcg aaatcctttt aaacaagaac gtagacgtag
    10681 gaagaacttg aaaatgaaca gaagttgttc attacggtaa attacaatat gattacaaaa
    10741 aacaacaaag ctcttaaata acttttaata aatattaaga gaagttataa ttattaataa
    10801 tagataaatt gaagttggag gatagaagat ggaaaacatt gcaagaaaag aacggagacg
    10861 tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg tgggaactgc
    10921 gatggtaggc tgttctgtcg ctgccccatt agtacagccg gttcctgttc aagcggatga
    10981 aacacctgtt cagttcagcg caagaatcaa tacagcggca tttatcacag aaattgctac
    11041 ttatgctcaa ccaatcgctc aagccaacga tctatatgca tcagtgatga tcgcacaagc
    11101 agttgtagaa agcggttggg gcagtagtgc gttatctcaa gcaccttatt ataatctttt
    11161 tgggataaaa ggcagctatc aaggacaaac cgtctacatg gatacgcttg aatttttaaa
    11221 taatcaatgg gtaaataaaa aggagccttt ccgacaatat ccttcttttg ctgaatcttt
    11281 caatgacaat gcctatgtac ttagaaatac ttcatttggc aacggttatt actatgccgg
    11341 tacttggaag agcaacacga aatcctatac agatgcgaca gcttgtttga cagggcgtta
    11401 tgcgactgat cctggttacg caggcaaatt aaataatatc atcgcaactt acggattgac
    11461 ccaatatgat acaccagcta gcggaaacgc aggcggtgga gcgactacag gtggcaataa
    11521 tactggaagt gcaggtagta ctggcggttc aagcaattct ggtaatacaa actcaggttc
    11581 agcaacttcg accacttata ctgtccaatc tggtgattct gtttggggaa tctcaaataa
    11641 attcggtatc acaatggatc aattgatcca atggaacaac atccaaaaca atttcattta
    11701 tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat ggctcttcga cgaataattc
    11761 cggtaacaat accaattctt caggaaatgc tggtacatca aacggaggac aaacaactgg
    11821 tgcgaaatac actgttcaat ctggtgattc tgtttggaag atctcaaatg atcacggaat
    11881 tacgatggct caattgattg aatggaacaa tatcaagaac aatttcgttt atccaggaca
    11941 acagctaatc gtatcaaatg gcggttcttc taatgctgga gcaaatactg gctcaaatac
    12001 tgggaataca agtggctcta gttcgtccag ctcaagttct tctaatacag cttcaggaac
    12061 gaaatataca gttaaagccg gagactctgt ttggagcgtc tcaaataaat acggaattac
    12121 gatggctcag ctaatccaat ggaacaatat ccaaaataac tttatctacc caggacaaca
    12181 actgactgtt tcaaacagtg gatcaacaaa cagcacgtca ggtaatacag gttcgtcaac
    12241 caatacaaat aacagcagtt cagttaatac tggcagcagt tcgtctcaag gcacttatac
    12301 cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa aacggcatca cgatggatca
    12361 gttaatcgaa tggaataata tcaaaaacaa cttcatttat ccaggacagc aattgatcgt
    12421 taaaggtgga acttcggcaa atacaaatac aggttcaact acttctgcag cgaaaccaaa
    12481 tacaccgaat acgtctgcta cgacaagtac atcatccact ggtaatacga tgtataccgt
    12541 gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat catatcacga tggatcagtt
    12601 aatcgaatgg aataatatca aaaataattt catctatcca ggacagcaag tgattgtgaa
    12661 gaaaggttca tctcaatcaa caaaccaaca atcagttact ggttcgaaaa cctatactgt
    12721 gaaatctgga gaatctgttt ggagtgttgc tgacagtcat ggaattacaa tggctcaact
    12781 aatcgaatgg aacaatatta agaacaactt tatttatcca ggtcaaacgt tgattgtagc
    12841 taaataaaat agctgttcga agaaataacg attgaattcc acaaaaatat ttgttactat
    12901 aaggctactt aaatatgaca acacttgaaa aggaagtagt aaaaagactg aagtttcaga
    12961 gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa ctcgcctttg agttgccctt
    13021 gctgaatacc cgagtaagca agcggcggtg acggccgtta caacgttcga ggccttttca
    13081 ctaaggtaaa attaggtggt accgcgtaga ttacgtccta taaaaagagc aatcttttta
    13141 taggattttt ttatttcttt tgtgaagttt gagaaaacaa agcaaaagga aataaaaata
    13201 aacagattct gccggttttt tcgttatatt tataatagaa acaagtgttt tcaatagaac
    13261 aggaggacag caaatgagtt acaatcataa agaaattgag aaaaaatggc aaaaatactg
    13321 ggcaaaaacg aatactttca acacccatga tgatcctgaa aagccaaaat tttacgcact
    13381 ggatatgttt ccctatccat ccggtcaagg gctacatgta gggcatcctg aaggttatac
    13441 ggcaacggat atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc tgcatccaat
    13501 ggggtgggat gcattcggtc tgcctgctga acaatatgcg ctagatacag gaaacgatcc
    13561 agctgagttt acccaaaaaa atatcgaaac attccgacgc caaatcaatt ctttaggatt
    13621 tagttatgac tggaatcgcg aagtgaatac aactgatccg gagtattata aatggacaca
    13681 atggatcttt acgaagcttt atgaaaaagg tctagcttat gaagcagaag tagcggtaaa
    13741 ctgggtacct gaattaggaa cagttatttc aaatgaagaa gtgatcgatg ggaaaagtga
    13801 acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa tggatgctga aaatcactgc
    13861 ttatgctgat cgcttattgg atgatctaga attagttgat tggccagaaa gcattaaaga
    13921 aatgcaaaga aattggatcg gtcgttcagt aggagcaaat attgaattta aagtggctgg
    13981 tacggataaa tcctataccg tatttacgac acgaccagat actctattcg gtgcaactta
    14041 ctcggttctt gcacctgaac tagatcttgt tagagaaatt acgacccctg aacaaaaagc
    14101 tgctgtagaa gcttatatcg aagagacagc taaaaaatca gatctaaaga gaacggatct
    14161 tgcaaaagaa aaaacaggag tatttactgg tgcttatgca atcaatccag ttaatggcaa
    14221 ggaaatccca atctggatcg ccgattatgt attatcttca tacggcacag gagccatcat
    14281 ggctgttcca gcacatgatg aacgtgactt tgaattcgct caaactttcg gactagaaat
    14341 tcttccagtt atcgaaggtg gagatgttga aaaagctgct tacacagaag atggtacgca
    14401 catcaactct gaatttctag acggcatgaa caaacaagaa gcaatcgata aaatgaatga
    14461 atggctagaa gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac gtgactggct
    14521 attctcccgt caacgttact ggggtgaacc aatcccaatc atccattggg aggacggaac
    14581 agtcacagct gtcccagaag aagagctacc attacggttg cctaagacaa aaaacatcaa
    14641 gccaagcggt actggagaat ctccattagc aaatatcgag gagtgggtca atgtcgttga
    14701 tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca atgccacaat gggcaggaag
    14761 ctcatggtat tacctgcgtt atatcgaccc tcataacaaa aaagaactag cagattatga
    14821 aaagttgaag cgttggctac cagttgacat ctacattggt ggagcagaac atgcggtact
    14881 tcatttacta tatgcacgat tctggcataa attcttatac gatatcggtg ttgttcctac
    14941 aaaagaacca ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa acaacgaaaa
    15001 aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa cttacggtgc
    15061 agatacactg cgcatgtacg aaatgttcat ggggccatta gatgcttcga ttgcttggag
    15121 cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt tgatcgttga
    15181 tgagaacaat aaaatgcgtg accgtatcac tacattgaat gatggcaagc ttgataaagt
    15241 gtatcatcaa acagtgaaaa aagtgacaga agactatgaa aatcttcatt tcaatacagc
    15301 aatctcccaa ttgatggtct ttatcaatga agcctataaa gtggacgctc tgccttatga
    15361 atatatcgaa ggatttgttc aactactcgc accgattgct cctcacatgg gtgaagaact
    15421 atgggcaatc cttggaaatg atggtggtat ttcttacgca ccatggccaa catacgatga
    15481 agcagcactt gttgaagacg aagtagaagt tgttttccaa gtaaacggta aagttcgtgc
    15541 gaaaagcaat gttccccgag acttaggaaa agatgagcta gaaaaagcag cattagccaa
    15601 tgaaatagtg caagaatata ttgaaggcaa aacagtcaga aaagttattg cggtaccaaa
    15661 caagttagtc aatatcgttg ctaattaatt ggctctttgt caataaggac tgatgagttg
    15721 tgcaaaatca aatctgggcc agaatgaact tcattctgtc tcagattttt tgttatttca
    15781 aattgattaa ttgattgatc aaaaaggttt tgattttcat gttctcaaac acgttcaatg
    15841 tctttttctt ctgaatggtc ttgtaagaga tctctgtaat catcaaattt ttgtctatta
    15901 atcttagtat tttttgatgt ataaactacc tgaaattgag agtacatacc aaattcataa
    15961 attttttatt agtgggacta gtgattgtat ggattttgac gcataatgaa tgaagtatga
    16021 gaaaaggaga gacaaatgga tcaagaacta tttattatcg aagtattatc agctaaacaa
    16081 aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct tttaagctta
    16141 gtatttttct ctggtttgct ttttttcgga gctgtatcgt tcctatatcg ccatatgagt
    16201 gcagtagtcc gttcagctga agggatgcaa aaattaaata atttgtatca cttttcgtgg
    16261 ggagttggcg gaataacaat tgttgtgctt attgtttttc ttctacttat ttggctccgt
    16321 taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca acagttttta
    16381 cataataaac caacttctca agatgatgac tatctttatt ttgagaagac ttcgacgagt
    16441 caagcttttc ggatacaaaa aagtgattgc cagttattgc tgagatcact tgatgattta
    16501 cctatctact taggcgaaag caaatcaaaa ttcggaatga acacattcca tattttttta
    16561 ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc ctggtggata cctgacaact
    16621 aagatgagta cattagtcac tggttttttc ctcattcttt tagctggaac aatctattat
    16681 gggagataca ctcagccaaa aatgtcttct catataaatg ctgcttctca catgacaatg
    16741 aactcaacta acgatacgca tatacaaaag acaaatgaag ggttgttagc tgatcctaag
    16801 caaaaggtaa atttgacaac acaaaagata aatgatctca aacttgatac agagacgcac
    16861 tctctctata tgacaacaga tagtggtgct aattgggtat ttgtgccgat tgattcgcaa
    16921 tggttgcgtt ttggcgaata caccctgaca acggggctga ttcctgaagg ttattggatg
    16981 gacaacacat atgatatttc cgccgatttc tcatggttta tctattcttc tggggatgat
    17041 gtacgtctgc tgacttcaag agataatggt aaaacatggc aaacgtctat tatcacccaa
    17101 tcagttcagc cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg tctatttatc
    17161 tgtacatcag ccagtgggat gtctgcagaa actttgtaca tttacactac tatggatggc
    17221 ggaatcactt ggataaaaag tggaagtaca atgatcaatc agccagtaca aaatgtcagc
    17281 ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa gtttgtttta tacaaacaac
    17341 ggaggacgct cattcaaaga atcagttctg tcgattccgg aaaactatca gacagggggg
    17401 atagatcttt ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac gttagagaca
    17461 aaattttatt tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc ctgcctcttc
    17521 cgttcaactg ataacgggga aacatggcaa ttcgttcagc aactatcgca agtagaacaa
    17581 ggagattaaa gaagagagat tgtgagcctg acgttcagca ccgagtcaca agaaacaaat
    17641 tcaaaaaaca gcttctcata tttgttgaat agtgtaactc gtgattcgga gttgacaaga
    17701 gcaacaagaa gcgattttga agtatagctt ctcgtatttt acaagatcgt gtcttattgc
    17761 cgataggttg tctcttgaac accgtttatt cgtaaaaagg gattgtgaca agtcttttgt
    17821 cactacttac cgaacgaaac tgaaggagct atccaaaaag tatcattagt attattcttt
    17881 gtgtttctag tggcaccact agtacattta aaaaagctat cgatcatgga ctaatcatac
    17941 atgatcgata gctttttaga taatgtttgt tacgctatct gaaattgact attataaaga
    18001 ctggcataaa gcgttggtga ttggagtagt tcagaatgtg ttcctttttc gacgatatcg
    18061 ccattcttca ttacaaggat catgtctgca ttttcaatcg ttgacaaacg gtgtgcaatc
    18121 acaaaacttg tcctatcttt tgtcacagct tccattgctt gctgaatcaa tgcttctgtt
    18181 cgtgtatcta cgctagaagt tgcttcatct aaaataacga caggaggatt cgctagaatg
    18241 actcgagcaa ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt ttcgctagaa
    18301 atgatcgtat catatccgtc tggcagtgtt cgaataaagt gatcacattg tgcgatttca
    18361 gccgcttcga taatttgttc tcttgtggca tccattttcc cgtaggcgat attatcagct
    18421 accgttcctt caaacagcca ggtattttgt aaaaccatcc caaaagaacg acgtagttct
    18481 ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga tctttccttt attcagttca
    18541 taaaaacgca tcagcagatt gactaaagtc gtttttcctg caccagttgg accaacgatc
    18601 gctaccgttt gttttggctt tactgaaaaa tcgacttgat tcatcaatat tttatctgat
    18661 gtgtaaccaa actgtacatt tttgaaagct atttttcctt tcggttgttc taaaagtctt
    18721 gcatctggaa ggtctggaat ttcatctgtt tcatctaata tctcaaatat cctatcgatt
    18781 gctgccattg cggattggat cgagttgatc acataagaag cggtagaaat tggttcagaa
    18841 atctgattga tgtactgaag ataagcttgt aagaagccga tcgtcacacc acctgataga
    18901 acaagcatag caccgaatac cgcacttatg atgaaggcga gttgattaat gaaacgaatg
    18961 gccggataga tagcaaagtt cataaattga gcacgtttaa aagcgttgta ttgcttttta
    19021 tttacttcgt gtatagcagc ttctgcatga gcttgctgat tgaatgtttt gaccactaga
    19081 ttaccagcta agatttcttc cattcgatta tttaaaacac ccagttcata ctgattctgt
    19141 tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa caattgaact gccaacgatc
    19201 agcaataaga cgagcaaagt aagcttgctt tcgatataaa acagcatcag accagcagca
    19261 gctattgtta cgacagaagt aaaaaattga ttgatacctg tcaataacac ttgagaaagt
    19321 tgattgagac cggtagttgt ccgactaatg atgtcaccta cttgatgatt gtcaaaaaac
    19381 gacatcggta atgttttgaa tttttttgtg atttcttttc gcaccctcaa tgtgatcttt
    19441 tcgcttaacg ctgccattgt ccgttcctga ataaatgaag tgatgctgct taagactgaa
    19501 cagccgatca gcaataagac agggaacaac aatgcttgtt tgatatcact gaaagaaagc
    19561 tgtccaatac ctgtttttct tatttctgat aagagatcat cgatcccgat tcccatgatc
    19621 aagggcatgg ctacgactaa taggttcccg aataaactgc atagaaacaa tccgtaaaaa
    19681 tatttatgtt caggtttgat cattttccaa aaacgtatca aagcacgcgt gttcaacgtt
    19741 tttttctttt ccatcgtatc ccttctttct atattccttg tgattttgca aactcctgat
    19801 aagtttggca attagacagt agttcttcat gagtaccttg tccaacgatt tttccttcat
    19861 ctaaaacaat gatgttatct gcttgttgga ttgtactgag gcgttgagca acaatcacga
    19921 gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct gttttatagt
    19981 ctaaagcgga gaaactatca tcgaatacat agatctctgc tggtttgatc aacgctcgag
    20041 caatacatat ccgctgtttc tggccccctg aataattaga accgccttga gcaacaaagc
    20101 tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc ttgatcgctt
    20161 tagacaactc ttctgttgag gcatattcat ttcccatctg caagttgctt aaaattgtcc
    20221 cgctgaataa gaaagccttc tgagggacat aactgatgtg ggagcggatc tgttcttgag
    20281 tcatttgagt aagaggaata ccaccaaggg ttacttttcc tatcgtaggg tcactaagtc
    20341 tcaagagaag tttcgccaaa ctactttttc cagatccggt cccgccaacg atcgctgttg
    20401 tttttccttt aggtattgtg aaatcaatat cttctagaac tggatcagag atttcagtat
    20461 atttaaatgt gacatgttca aaagcagctg catttgtctg atcaaaggtt ggttcagcta
    20521 ctgttttgat cgtctcttcc tcaatttcga tctctgcttg cagtatttct tctatccgtc
    20581 gtaatgaggc aaacgattta gggataatga ccaataccat agctgcgatc atcaagtaag
    20641 caagtgtcag catagagtat tcgactacag cagtcacgac accgatttgt aatgctcctt
    20701 ctgctacaag atttccgcca aaccacaaaa tagaagcata aacgattccc atgagcaaaa
    20761 aagcaattgg agtgacccaa gcaaatattc ggttcacttt gatcatttga ttagcatagt
    20821 cagcaaagct cttattcgtt cgcttttctt catatgattg attatcaaag gcacggatca
    20881 tattaatccc agtaaagaat tctctcaatg tgatggtgat ccgatccatt tttggctgga
    20941 tactcaaaga taaaggtgcc cctcgtttca tcagtcgata aacaatccat acataaaaaa
    21001 caacagagat caatgtaacc catgttagta atagagaata acgaaacatc atgagaatgg
    21061 tgaaaacgca aataaccggt gctggtaaga tcatttggca aaataaaaca atcatttgct
    21121 ggacattatc gacatcattc gtcattcttg tcaaaagaga tccagaacta aaacgactga
    21181 tatccttcat tgacagctgt tggaatttac ggaaatattt ctcccgcgtt tggaatccaa
    21241 acctagcgga agccaatgct gaaaagtaac tagaggcaac tgcagcgatt gttcctagaa
    21301 acgctacaag cagcatttca actccaattt tttttataac tgaggcttcg ccacttgcta
    21361 tacctgtgtc gatcagcttt gctactaatt ttggtacacc taatgtaccg gcgatttgta
    21421 aacagatcat cacaaaggta aggagagcga gtcctttgtt tttctgtaag aatgttttta
    21481 agatagacat attattcctc cttttttcct gatacttttc gattctatac cttcaagtaa
    21541 cttgaaggtc aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa tgaaaaaaat
    21601 gtatacgatt ggccaagtat cggaactatt tgatttgcca aaatcaacga tccgctattg
    21661 ggatgaacaa ggattaatcc gttcaagtag acaagaagag aatgattatc gattgtttga
    21721 tatcgatgat atttttatgc tttatgatat cgttttttat agaaaattag atataccgat
    21781 caaacagatg aagaatttat atggaaaaac attgacggag ttatatgaga cacttgatga
    21841 aacagaaaga agaatccata aagaactggt ggtgatgaaa caaaaacaaa aagagataag
    21901 ggagcaaaaa aaacagttga aattgatgat cgatacaaac gaggaagaat ttcctgtaga
    21961 agagatccct tttgactgta tgatctcaac ggagtttgaa gatattgtgg aaatcaaaaa
    22021 atttctacct aactattcaa gctttgggat gatgagtatg ccagcttcca gttcacagac
    22081 gatgtatgga ttttttatcg accctagtga agtacattta tttactcaag acgtgatttg
    22141 ggagaaaaaa gatacggctg tttatcgtcg attcttattg aaatccgaga tgaaccatgc
    22201 ggaacgtaat aatattttag aaatcagaga aagaatgtat gaaaaaggat ggaaaacagg
    22261 agaagtgatc ggccagtatc tattgacgaa tacggatgaa aacaatatca gaactgaata
    22321 ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt gtttaagata actcgaaaat
    22381 gatggttgtt ttagttataa agaaaaattt tctggttcga ttacgggttt aatgtggggg
    22441 attattagtc gtggatagat agaattttag ttttttagag gaaatatgtc tttaaaaatg
    22501 gaatggttgc aattcttagc gatatatacg atgtaattgt cctcttaaaa aagtatggaa
    22561 aaataattgt attgggtgtt atgtataatt catggtgaac tgagactaat gagaagaaaa
    22621 attcatattg ttatgagtga gattgtatca gatgagaatg ataaattaaa taggtgcata
    22681 aaagcattac acttaaaata cattgttgct tacattatta caatatgtta ataaggcaaa
    22741 aataaataga tggattgaat aaacatattt tttaatcaaa tagtcccta
//
LOCUS       DACESX010000038        22687 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.038, whole genome shotgun sequence.
ACCESSION   DACESX010000038 DACESX010000000
VERSION     DACESX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.038"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(189..2270)
                     /gene="cadA"
                     /locus_tag="IXF88_001215"
     CDS             complement(189..2270)
                     /gene="cadA"
                     /locus_tag="IXF88_001215"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1507955.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(2794..3153)
                     /locus_tag="IXF88_001216"
     CDS             complement(2794..3153)
                     /locus_tag="IXF88_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1507956.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3183..3524)
                     /locus_tag="IXF88_001217"
     CDS             complement(3183..3524)
                     /locus_tag="IXF88_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507957.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(3521..4195)
                     /locus_tag="IXF88_001218"
     CDS             complement(3521..4195)
                     /locus_tag="IXF88_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1507958.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5372..6670)
                     /gene="asnS"
                     /locus_tag="IXF88_001219"
     CDS             complement(5372..6670)
                     /gene="asnS"
                     /locus_tag="IXF88_001219"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1507959.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6710..7900)
                     /locus_tag="IXF88_001220"
     CDS             complement(6710..7900)
                     /locus_tag="IXF88_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1507960.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7921..8448)
                     /locus_tag="IXF88_001221"
     CDS             complement(7921..8448)
                     /locus_tag="IXF88_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1507961.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8495..11284)
                     /locus_tag="IXF88_001222"
     CDS             complement(8495..11284)
                     /locus_tag="IXF88_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1507962.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11431..11631)
                     /locus_tag="IXF88_001223"
     CDS             complement(11431..11631)
                     /locus_tag="IXF88_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1507963.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(12083..12403)
                     /locus_tag="IXF88_001224"
     CDS             complement(12083..12403)
                     /locus_tag="IXF88_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507964.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGYHPV
                     IYEV"
     gene            complement(12787..13905)
                     /locus_tag="IXF88_001225"
     CDS             complement(12787..13905)
                     /locus_tag="IXF88_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507965.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            14376..15683
                     /locus_tag="IXF88_001226"
     CDS             14376..15683
                     /locus_tag="IXF88_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1507966.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            15802..17001
                     /locus_tag="IXF88_001227"
     CDS             15802..17001
                     /locus_tag="IXF88_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1507967.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(17028..18296)
                     /locus_tag="IXF88_001228"
     CDS             complement(17028..18296)
                     /locus_tag="IXF88_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ1507968.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(18459..19106)
                     /locus_tag="IXF88_001229"
     CDS             complement(18459..19106)
                     /locus_tag="IXF88_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ1507969.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(19514..20017)
                     /locus_tag="IXF88_001230"
     CDS             complement(19514..20017)
                     /locus_tag="IXF88_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1507970.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(20140..20904)
                     /locus_tag="IXF88_001231"
     CDS             complement(20140..20904)
                     /locus_tag="IXF88_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1507971.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            21012..21287
                     /locus_tag="IXF88_001232"
     CDS             21012..21287
                     /locus_tag="IXF88_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1507972.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            21725..22675
                     /gene="yidC"
                     /locus_tag="IXF88_001233"
     CDS             21725..22675
                     /gene="yidC"
                     /locus_tag="IXF88_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1507973.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         6532 a         4816 c         4019 g         7320 t
ORIGIN      
        1 atattttttc catcaaaaaa ataagttatt ttcttttatt tcatttacct tattgaattt
       61 caagctattt tgaattttct gcaaaattaa aaacattagt tattttataa tcctatctat
      121 tttctttaca tttttcatta agaaaaggat ataaaaaaaa gaggacgttc attcgtcctc
      181 ttcattgatc aacgtttatt ttttacatgc aagcttcgca tggcattcaa aacagctaat
      241 actgtcaccc caacatctgc aaatactgca tctcccattg atgcaaaacc aaaagcacct
      301 aaggcaagta ccaaaatttt tatcccaata gcaaagacaa tgttctgttt gacgattttc
      361 aatgttttct ttgctaaatg gattgcttct gcaatcttcg atggttgatc attcatgatg
      421 actacatctg ctgcttcaat tgcagcatcg cttcccaatc cacccattgc gattcctaca
      481 tctgctcttg ctagcaccgg tgcatcgttg attccatctc cgacaaatgc ggtcttttta
      541 ttgtttcttt gtaagatttc ctctaaccgc tgcactttat cttctggaag caattcgcta
      601 tatatcttgt ccacgcctac ttgcttcccg atatgatcag caattttttt cgaatcgccc
      661 gtcaacatga ctgtgttctt cacaccagcc ttttttaatt gaaccaatgc gtctacggca
      721 tcttctttca aggtatctgc aataacaaga taaccactgt aactttgatc gatcgctaga
      781 taaagcaatg taccgatttc ttgacttgaa ggaaaagaga tttcaaatct ttccatcaat
      841 ttgtgattac ctgctaatac ttgtttgcca tcaacttcag ctaagatccc ttcacctgca
      901 aattcttgga tattagctgt tggtaaaagc ggacctgcgt agccttctaa aacagattga
      961 gcaatcggat gtgtagaaaa ctgttctgca ctcgcaacat attgcatgaa tatctttgga
     1021 tcgatttttg tatcgatcgt ctggatcgaa aagtcacctt tcgttaatgt gccggtttta
     1081 tcgaatacca ctgtctcagt ttgtgcaagc aactccaagt aatttgaacc ttttactaat
     1141 acaccgattt tacttgctcc tccgataccg ccaaagaaac taaggggtac ggagatcact
     1201 aatgcacatg gacatgagat cactaaaaat gtcaatgctc gatagatcca ttcagaaaac
     1261 gccgctccag aaacaacaag tggaggtaaa acagctaata aaacggccaa tcctacaacg
     1321 actggtgtgt aatagcgcgc gaatttagta atgaaatttt cagctggtgc ttttttactg
     1381 ctggcatttt ctactaattc taataatttg ctgatagtag aattttcata agatttttct
     1441 acttgaacta aaaatggttt atttttattg ataaatccac ttaatactgt ttcaccaaca
     1501 tatacagatt tagggactga ttctcctgta agtgctgacg tatcgactaa tgattctcct
     1561 tcgatgatcg ttccatctaa cggtacacgt tcaccaggct tcacaagtac aaactgaccg
     1621 atctgcacgt cttcagggtt cacggtcatc aatccttccg tcgtctgcac gtgagctgtt
     1681 tctggacgga tcgccatcaa ttcgcgaatc gattttcttg aatgactgac tgccaatcct
     1741 tggaaatact cgccgatttg ataaaatagc atcactgcta cggcttctgg atattcaccg
     1801 atcaacatag cgccaatcgt agcaacagac ataagaaaat tctcatcaaa gatttttcct
     1861 ttgacgatat tcatcactgc cttcttcact acatcatatc ctatcagcag ataaatcaat
     1921 aaatacccta tcaagcgaat tggttcatta aaatcaaata atagcaataa agcgatacct
     1981 gcaagggaaa ataaaatccg gaacagcgta tttctctctt tttcatttcc atgttcagat
     2041 actttttcct tatctatttc tgttactttg acatctggtt ctaattcctt cactactttt
     2101 tttatttctt gttcaatcgt agccaaatga tcttcctctg cgtcaatcgt tagttttcca
     2161 gaagtgaagt ttaccgttgc ttccttgact ccattgatct tctgaactcc tttttcgatt
     2221 ttcattgcac agttcgcaca atctaaacct tctaatctgt aacttttcat tttaatgacc
     2281 tcctcgcctc aacatatgaa tatctcttca taactaatta taaatgaacg gttattcata
     2341 tgtcaaggta tccgaaagaa aaaatgaaaa acatctgcat cggaaaattt cttccataca
     2401 gatgttttgg cttattcttt ctttttcttt tcgatcattg ccactgctct tatccaaccc
     2461 gcacttgcct tcttgatctt gacaggatag caactaagtt tgaagcctgt tgctggcaat
     2521 ttgtctaaat tcattaattt ttcgatagga caatatcttt tttcaatccc tgcaaaatgt
     2581 ccttcccaga tattcgaagc tccactacgc tttcaaaaat atagaaagag ttctttaatg
     2641 aaaattattt caactttcca taaacagtaa ccccatataa ataaaagacg taaaagtcac
     2701 atggaagaac ttatacagga gaacgcatag agtcaggtga aatacatgta tcccacctga
     2761 ctctatgctt tatctaacct ttattattct cttttataca atttctgcac cgaatggcat
     2821 caacgccaat cgagcaagtt tgaaagattg cttggcaaaa ggtatcccta tgatcgtgat
     2881 acatagaagg atgccgctga cgacatgtgc gagagcaatt ggtaggcctg aaacgatcag
     2941 ccagatgata ttaacaatca atgacatgcc agactggtga taaattactt ctttgccaaa
     3001 aggcgcaaaa gaaagggagg cgatcttaaa acattgcata ccgattggga tacctatgat
     3061 cgtgatacac caaagtatcc ccgctgctaa ccagctaaga ccaccgatta gtccgccgaa
     3121 tataaaccag atgacatttc ctaaacaact cattgtattt ctccttctat tactgttttt
     3181 gattaaaact gggcaaaaaa tgattttatt cttctttttt tagcttccag tatcaaatat
     3241 tctgcaaatt ttttttggat ttcttccgta ttttcttttg ttactaatgt gtgttggatt
     3301 agttttcctg atggtcgctg atccaatcgt tcttctgact gttgcacgtc cgcttgcgca
     3361 aatccgataa tccgtttttc ttttgttaca gcaacgagca atagtttatt cttatcattt
     3421 aggtcagctg ccgtttttgc ttttacgaat gattctggat gatcagtaat cagctgattg
     3481 atttgaggta gatcttcttt tactgcccac cgtatttcca tcatttctca cttttctttc
     3541 gctcattttt atctccacta aaaaaatcga ctgcggcaat tgcatctatc cagctttgtg
     3601 caatgatctc atgtccgcca atcgttgggt ggaccccatc ttctcttgta tatttcgaaa
     3661 aaccataaac aatccctaaa gcattgaata taccatctaa aggaaccagc aatgcgccat
     3721 attccttagc aagtttacga acaatttgga ttttagggtc taagtcttta cgccagccat
     3781 gacggtcgtc ggttatagga agtacaaacg gctccatcaa gatgatgttg cagattccca
     3841 ctgtttgaag ttcatttagt aattttcgat aaacggattc aaattgctga gcagatttct
     3901 cagttccgaa tatagcctga tcgctagtat tatgccaggt atcattgatt ccgatcaaaa
     3961 tcgatacata gtcaggttgt aaagctaaac aatcttcttg ccagcgtact tgcagatctg
     4021 caattttgtc tcctcctatc ccacggttca tgcattcaaa ttccatcgtt ggatattggg
     4081 agattagttt tccggcaatc aaaagtggat aacctttacc tagatcagtt gcttctgctc
     4141 ggtctcttcc tgcgtctgta atgctatcac cgataaataa tatacgtttt ttcatttgtc
     4201 aaaaactcct ttttgaataa aacgatgatt ttcttcctta tattatccca taagccctat
     4261 caagacgaaa gagaaaatat atgtataaaa aatgacacca gcctagtccc ttcaaacaat
     4321 tgaatgaacg ctcatcaagt gtctagtttt ttatgctatt tttttcttaa cgtgccggat
     4381 tccccttgat tccttcgttg tttggtccag agagatcgat cacacctaag cttccgtcaa
     4441 tcagaccact gatgacgcca tcagaacata tatttatagg ctcatctaag acttctagta
     4501 aatgtccctt tactaatatc tcttctagat tgcttgggat agattggtca taactttatc
     4561 tggtccgatg atttcaaggg tgaattgaac ttacatatgg attattgcat gctgaaaata
     4621 cggacctact ccatcttcga atgttactat tagtttgttt tcaggtgcta gatgctgctt
     4681 tatttcaaca gcagaagcat catcaaatca gatttccatt tgaattacct tctttgtaaa
     4741 tgtttgattg attgcgattt cgttatagaa ccttgtgaat caaatagcaa tttcttttgc
     4801 tttcttctct tgtctgctgg aaacttcctt tatcccgtat tttaaaaaaa gaactgcgag
     4861 agttcgcagt tcttttttag aataaacggt gctctccggc caactcctcg gtcacaagtc
     4921 acaatagtca tcaaacatga aaaactgttt tttgaaatta ctccttgtga ctcggagctg
     4981 aacggcaggc gcacaacctc ttgatataaa cggtgttcaa tcagctgacc ctcgatatta
     5041 gctaaaaaaa cggtaaaata tgagaagcta tttttccgtt ttgtttctct aatactcagg
     5101 tcaaggcact gatttcacaa cctcttcctt aaacggtgtt caaaagccag tttctcggta
     5161 ttaagccaaa tttatgcaaa aatgtggaaa gccatttcca caaaattttt cttaatactc
     5221 gaaactaaac agctttttca caacctctcc actaaacggt attcaaaagc ttacatctgc
     5281 ggtaataagt cccgccaatt caaaaacatg aacagctgtt tttgcttggc tgttctaatt
     5341 acttgatgca gaacagcttt ttcatcacct cttatggata aattcggttc aacaaacgtg
     5401 ggaatggaat agcttcacgc acatggtcga taccagcaat ccaagtgact gttcgttcaa
     5461 ggcctagacc aaaaccagag tgcggaacag aaccgtattt acgtagatca agataccatg
     5521 aatattcttt ttcatccaaa ccgtgattac ggatttgttc tagcaagaaa tcatagtccg
     5581 ttgcccgttc gcttccgcca ataatttcac cgtaaccttc tggtgcgatc atatctgcac
     5641 aaattaccac gtcttctctt gttggatgtg gtttcatgta gaatggcttg atagcttttg
     5701 gatagtttag gataaagaca ggctgttcga atgaattcgc aatgaacgtt tcatgaggtg
     5761 aaccaaagtc ttcgccccat tcgatgtcat caaatccatt tttcttcagt aattcgatcg
     5821 catcatcata agtaatacga gggaatggca attttgtata agtttttaag acttctttgt
     5881 ctcgtcctag tacatctaat gcgtattcgc aatgatctaa aacattttgt actaagaatg
     5941 ctacatattg ttcctgtact tctaaacttt cttcttgatg agtgaaagcc atttctggtt
     6001 cgatcatcca gaattcaatc aaatgacgtc gtgtttttga tttttcagca cggaaagtcg
     6061 gaccgaatga aaagactttt ccgaaagcca tagcagcagc ttccatgtat aattggccac
     6121 tttgagataa ataagctttt tgatcaaagt aatttgtttc gaatagatcc gtagtgcctt
     6181 ctggtgcgga accagtcaag atcggcggat cgattttgac aaaatgatgt tgattaaaga
     6241 attcatacgt tgcacgaatg atttcatttc tgatctgcat aatcgcatgt tgtcttgaag
     6301 aacgtagcca taaatgacga tgatccatca agaagtctgt tccgtgttct tttggtgtga
     6361 tcggataatc gtggctctcg cttaccactt ccacatttgt aattccgatt tcataaccaa
     6421 attttgaacg gctgtcttca cggatttctc cagttactaa cacggatgtt tcttggtgca
     6481 gatttttagc tgtttggaaa acttcttccg gcacttcgct ttttacaact acaccttgga
     6541 aaaaggctgt tccgtctctt aattgtaaaa atgcgatttt tccgcttgat cgcttatttg
     6601 cgacccaagc gccgattgtt actgtttctc ccacatggtt tttcgaatcg atgatttgaa
     6661 tttgttccac gtatgactct ccttttatat tccttgaatt tcttcaggat taattttgac
     6721 ttttactctc aataaattgc ttgattctgc gtaccgcttc ttctaacgta gcgcgatcag
     6781 tggcataact catgcggaca ttttctggtg ccccaaagcc ttcgccagtc acaagcgcta
     6841 cccctgtttc ttctaaaaga tcttctaccc attgtgtgac attttcatat ttacacatgc
     6901 gtagcgtttc ttttacatta ggaaaaagat aaaaagctcc ttgtggcttt tcaagagaaa
     6961 cacccggcag ttctgccaca agcggatata acgtattcag gcgttcttca aaggcgttcc
     7021 gcatttcttc cactgtatct tgctcgcctt tcaatgcttc aacagctgca tattgactga
     7081 ctgctgtcgg attgctagtt gattgggaag caacagcgat cattccattg atgatcacag
     7141 gatttcccac cgcataacca atgcgccagc ctgtcatcgc atatgtttta gacacgccgt
     7201 tgatgatgat ggtctgattt ttgattgctt ctgaaatcgt tgcaattgga gtaaactcat
     7261 tgccattata aactaaacgg ccataaatat catcagcaac tatcaaaata tcatttttta
     7321 ctgcccattc accgattgcc tgcagttcat tcttgctata aatcattcca gtcggattcg
     7381 atggagaatt gatgattacg gcttttgttt tttctgaacg tgcttgttct agctgctcaa
     7441 cagtaacttt ccagctattg gattcttctc ctttcaaaaa gacaggaagt ccttctgcca
     7501 gttttacttg ctcaccgtaa ctgacccagt aaggcacagg aatgatgact tcatcttgcg
     7561 ggtcaaggat cgtttggaaa agggtataca aagcaaattt cgctccgtct gttacgactg
     7621 tttgtgaagg ttcatagaca agtccatagt ttttcttcag ataatcaacg attgcctgtc
     7681 gaagctctgg gattcctgct gttggtgtat aatagctagc tttcccattg cggattgctt
     7741 cgattgctgc ttcttggatg ttttctggtg tcgcaaaatc cggttctccc acagtcaaac
     7801 tcagcacatc tctgcctttg gcttttaatg ccttggcctt tgccgcagct gccaaagtga
     7861 cagacggttc taatcgtttt gttctttttg ataactccat cccattcatt cctttacctt
     7921 ttaaattccc ttgattgtct tgacttcatc tccagattca aagccaatca ggtaataatt
     7981 cagtttccca tttgtttctt ttcctactac ttcccacaca ggctgatcat catacatccc
     8041 taaagcagct ttttcgattt ggatttcagg atgggccgca gcaattgtct gttttgcttc
     8101 attttcagtc agtccctttg tttgatccaa tacttttact ttttcaccag atttaggaat
     8161 gataacggcg atttcttttc ctttatcatt tgtacctgtc agtgaaaagt atgtcttctt
     8221 tcgagtaaac caataaaaat gatcggcttc ctcaagattt gcatatttct ttgcgatttc
     8281 tactgcttct ttcttcgcct gcaccgccgg acgactggaa cgcaagtaaa aaatcgagct
     8341 gatcacgatg accgctaata aaaatacgat caagccaagt aacacgcgat taatcgtttt
     8401 ttcctttgtc tgttttttct tcaagggtcg tcgctccttt atcttcattc cttttcatta
     8461 tagcagattt taattatgaa aaaagacagc tcctctaact tccttcttca tttttcttta
     8521 agaattcatg gatatcctga tagatttcct ccatagctag ctctttgatc ggtacttttt
     8581 tcggtaatgc atggatgatc cgtgcaccat attttgcagt aactagtctt cgatccaaaa
     8641 taagcaatac gccacgatct gtctccgtcc ttatcaagcg tcctaatgct tggcgtaact
     8701 tcaaagctgc tttaggtacg gattcctggt aaaaaggatt gatcccctct gattctaaat
     8761 aagcgttcct agctttgacc atcgggcgaa gcggattatc gaagggtaaa cgtgtgacta
     8821 caacgatctg caaagcatct ccaggcaaat ctacaccttc ccaaaaactg tcagcgccaa
     8881 acaatacgct tttttctgac aaaatgaagc gtttcaaaag cttctcccgg cttccgccaa
     8941 acccttgagc tagtatttct cgtccttttt ctagcataga cagatgcatt ttctgataga
     9001 ccttctgcaa aacatcatga gaagtgaata acacaagtat tggccgatct tcttgttcga
     9061 tcagacctgt aagtgctgtc tctaaataat cgatatatgc ttgattgctg acttgatgga
     9121 cggcaggtgc ctcttttggc acatagatcc ttgcctgttt ctcataatca taaggagagg
     9181 caatcacttt aagaggtgct gcttcaatgc caagccgctt agagagatag tgtcgattag
     9241 ggccgatctt cagcgttcct ccaatataaa gaatacgatc atatcgagga taccaaacag
     9301 tcgacggcaa catagccgct tgaaaatcat ggagatgaaa aatcatctgc tgcgctgtgt
     9361 tcccaggaac gaaccaatgg atcaaatgat ctcgccagtc ttccagccat tgatccatca
     9421 gcaagtactg cttgtctaat tgatcaaata agctatataa acttgctaaa tcgatccgca
     9481 tctggtttgt ccagctttct tggataatct caaaagtcag acgcatttgt gactgtagct
     9541 gctggatctc ctgataaaaa agctgcaggt gatcaatggc tgtcccagat tccagtggca
     9601 gctgatccaa catctctttt gtcgtcatga cttcttctgg atactgtccg ttaggttgct
     9661 tgccgctcac taaactcatt aatccttctg ccatacaaat ctgtgcttcg catagcgcag
     9721 aaagttcttc tcgataaatc gataaccagc gtaaggcttc atgttctttc ggtaaatagc
     9781 tttgtattcg gtcaaacagc tgatttggtt cttctaattg ctggatttgt ttatggaaat
     9841 agttaatact taatcgtttg tcatttactt tttcagcgat ttccggcaaa tgatgtactt
     9901 catcgatcaa caaaaatgga ctagctggaa gcaatggttc ttttcgctgt gtttcttgta
     9961 ttaaaaacgc atgattgaca atcaaaaaat tactttgcct gattttcttt tgcaagaaac
    10021 gaacaaagtc ttgttcgtaa aatgactgat ttcttgctaa aaatccagtg cctctgtgcg
    10081 cgatatctgc aaagaatgaa tggttcaaat ttgttaaatg taattcatcc aaatcaccgg
    10141 tcttcgtttg cgtcagccaa actaagaccg ccatttgata caaagcatat tgtttttgtt
    10201 ctggcggttc cacaagtgtg cctttaaaac gctgtaaatc aatatagtgg cgatagcttt
    10261 tgataattac aggttgtatg ggctgatcta gcaattgatt cagcaatgga atgtcttttt
    10321 ctatgatctg ctgctgcaaa actagcgaaa cggtactgat gacagcgggt ttttcaggtg
    10381 tagccaaata actcaatggc agcagataac cgattgtttt acccattcct gtagctgctt
    10441 cgatcatcaa atcttttgat tcatcttttg tgaaatggtc gtagaccgca ttcatcagtc
    10501 tgttctgttc ttttcgatac atcagtgttt taccaaacat tttttctttt gcttgttttt
    10561 ttcttggata tgtccgctcg ccataatgga cgtatatgaa tagttccact tcttttttct
    10621 gcaaagctag cccatcgacg atttctaatg agggatcaag tggctctggt ttttctttca
    10681 tttcttcaat cacatgataa atgaaacgac tcgtatccat acctgtgtgt ctacttaatc
    10741 gcgcaatctg ctctaacgtg atgattggca attttctcat ccgttcttcg atcagcaaaa
    10801 gaagttgacc cgtcacttcg gcatcactat ctgcctgatg aggattctca tgaataaatc
    10861 caaggctttc tgacaaatca gctaagcgaa agcttggttc cgtcggcaag aaaatttgtg
    10921 caagttctac tgtatcgatg cctggtatct tcaaactagg aagtccgcat cgcatgagtt
    10981 catggttcaa aaaattataa tcaaaataaa tattatgcgc gacaaatatg gtgtctgcca
    11041 aaagattgta aatcgtttgg gcgacatctt cgaaataagg cgctttttgg acgcgttgat
    11101 tcgtgatccc tgttaaatgc tggatttgtt ttgaaatctt ccgccctgga tgaatatcga
    11161 tcgaaaaacg tgaagtgatc cgacctgatt caatcatgac gcagccaaat tggatgatcc
    11221 gatcttcttt tggattcgtc ccagtcgttt cgatatcaac gactgcataa gttcttggtt
    11281 tcaaaatatc ttcctcttct cctgcctcta atgttctttt gaaattatac tacaaagtgc
    11341 agaaaaatac tctcatcgct ttttaaagaa aatagacaaa aacgcccaga cacgcaaatg
    11401 tctgggcgtt tattttttag tataggttaa ttaagcttta gtaacgtttg ttgcttgagg
    11461 tccgcgttgg ccttcttcaa catcgaaagt tactgcttgg ccttcttcta aagttttgaa
    11521 tccgtcgcct tggatagctg agaaatgaac gaatacatcg tttccgtttt ctgcagtgat
    11581 gaatccaaat cctttttctg agttaaacca ttttactgta cctgtttcca taataaaaat
    11641 cctccttgtg tatcaacaca tattttgcaa ttacttgaat ggtgtatatc tggaagatgt
    11701 ttatttgaaa caagctacca cagattaccg aacaaaaact actcctatag tatatcatac
    11761 cgtttacaaa aaagctagag tttttcgact ttttttatta aaaaaagttt tctcagcgat
    11821 tacataaagt gaaaaaccga acttttttta aaataaacga catattgttc agttggtgtt
    11881 tttttgctct gatgttcttc tttaaactaa aaaaattatt ctttctgact acttatcggt
    11941 tctgtttcaa cggtcgaggc tggggaagat tctttagtag gtctgatgtt ctcatggtat
    12001 ttctgatagc ctatgacagt taccagaaga acaaggcaaa ccaatacgat caacgagatt
    12061 agtttttctt ttttgctcat atctatacct cataaataac aggatgatat ccatcatatt
    12121 tctgagtatg agtaaaagca aaagctgcag ctgtagaatg taagaaatgc cagcaacttt
    12181 cataatcaca ggtcggacag taccaaaagt aaccaaatct tggctgcaaa gaccgttctt
    12241 ctgtttcagg aatcgcagaa tcaagatctg ttgtctgtac ataaacattt ttgtacagac
    12301 gtgtgcctgt aataggagac cgctgtgttt cttcagcaaa aactggctga cttgttaaac
    12361 ttagggatag aaacagcaaa caccataaca gtcttttttt cataaagacc tccttatcga
    12421 taagtttttc ccaattaaaa tcatatctca agaacgcccg ttattcacat tttgttcata
    12481 aatgatgttt tttgtatcat aatcgtcaaa aagaggtcgt gaccacgtca caacctcttt
    12541 tttaaacggt gttccagaag tagcttcttc ggaaataagt cgaaatttca caaaaatttg
    12601 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
    12661 cttccttaaa cggtgttcaa aagcttgcat ctgcggtatt agctcaaaaa acggaaaaat
    12721 atgtgaaact atttttccgt ttgtttctct aatactcagg tcaaaacact gatttcacaa
    12781 cctcttttaa taacgaagaa tcgctgtcag actcttttca tctggcgcat ctttttgatc
    12841 caaaataaac actttcccgc cattaatgat cacatcatct gctaatgcat tcaacacttg
    12901 acgccgatca tattctgttt caggatcttc accgaaacca tcagctaagt ttgacgtagc
    12961 gataaataaa tgtgacactt ttccttcttt cgcagctgga acgatatcaa ctaactgatc
    13021 gatgaatttc ttatctaaga gtttattgta ttcttttgat tcttgagctg cgagttcact
    13081 tgacagcttc ttcgcccctt cttcgatatc tctaaaggat agctgcgcag gagaagcaga
    13141 aatcgcaacg tctgcacaat aatagggatt tttagcaatt tttttgaaca tcgtctgatt
    13201 ttctggtaat gcaaataaat aaatcggcca ttgttcagga ttttcgaaag aatccttcaa
    13261 gtacgtatca acagcttggt aatagttgac ccaatcgatc tctacttctt catcttttgg
    13321 atttacgcca tggaaagtta ctccatctcg ggctccgcct cctccattag aagagaagtt
    13381 caaaccgcca cctgtcagtt cagatcccaa tgcagtaacg acatcaaccg gtgccccttc
    13441 tggtagagat acttctttta cttgtttgtt gctgacctca tacaatttca tggagtcgcg
    13501 gttcaatccc aataaataat aagaatagtt gaactgccca tttttgataa tagccagtaa
    13561 ataaggaaga tcacttacat agtattgatc gtctaccggg atactcaaac gatggatata
    13621 ggtctggtca gcagttataa tgaccgctac actcgtagtt gcactacgcc aaaacgaagg
    13681 atctcctaac aaattttcaa ttttttcttg gaacggggac cagtcatgtt cggaatattt
    13741 tttctcaaaa cgtttctttg cttctttcgc aaaatttttg aatttgatct gatctttttc
    13801 cacatcttga tgggcgacat gcgtattcag catgatcgtg acgaatggtc ctttagcgcc
    13861 ttctgaagtc aattgtgtca ataattcttt atcctttttt gccataaacg tacccctcct
    13921 aaaaaaatca ttcctttgtt tctatctaaa gtctaccaca acactatttt tccatacaat
    13981 aaaacgtatc taaaaaaata aaacggtttc tacggtttta aatttagcta tttttttaag
    14041 ttatcttcat aatttcctca attatttagc cccagtcttc aactattctt catgaaatgc
    14101 tgataaacta agaaaaagaa taaaagaggt ggatcttatg catttgatta tgattttcag
    14161 tttattaagt attatcagtt tatttgcttt cagcgcttcc cgcttattag atgcggaaac
    14221 acagcctatt cctgtaaaag tacgtaaaaa cgaccgctaa acttccataa cgcgcgttct
    14281 tatcctaaag ttttttcaga aacattgaaa ctctctcccc tctctgataa aattttaatt
    14341 tgtagcgaaa caaattaatt acaaggagat cggatatgtc cataaaaaaa acacgtctag
    14401 tcctattatt ggctagtctc attctttgcc ttgcttcttt tctaccagct gttgctgtgc
    14461 aagctgaaga tacgttcaaa gtaaatgcaa aagcagcttt tgctgtagat gcgcaaagcg
    14521 gtaagattct ttatgatcaa gatggagaaa aaaccatggg gatcgcttca atcacaaaaa
    14581 tcatcggtct ttacatcgtc cttgatcaag taaaagaagg aaaactgtca tgggatgata
    14641 aagtttcgat ttcagattat gcagaaaatt tgagtatcac cccagattta tcgaatgttc
    14701 ctttgcataa agaaaacacc tacactgtca aagaattatt cgactctgca atcatccagt
    14761 cagccaatgc ttcaatggtg gccttggcag aaaaaatctc tggcagcgaa gctaaattca
    14821 ctgaacgaat gaaagaacaa ttgaaagatt ggggaatcaa agatgcaaca atcgttaatg
    14881 catcaggatt gaacaactct tatcttggcg aaaaccgacc agaaggcact ggagaaaacg
    14941 acgaaaatca aatgtctgct caagatgtgg ccatcgttgc ccgccatttg atcttggatt
    15001 tcccagaaat actggatgta tcaagtacta cgacccaaat gtttggcgaa aatacgcaat
    15061 caccagtgga aatggtcaac tggaactgga tgctcccagg attcgttaat tacaaggaag
    15121 gcgtggacgg attaaaaact ggtacgactg agcttgcagg tgcttgtttc gttggtacga
    15181 tcactaaaaa cgatcaacgg atcatcactg tcgtattgaa tgcggacggc catgcggaaa
    15241 acccgagtgc ccgcttcaat gaaacagacc gtttgatgga ctactgctat gacaattggt
    15301 cagaaaaaga gctaggaaaa gctaacgcaa gtattcctag tctaaaaacc attgatgtaa
    15361 aagacggaaa agaatcaaca gttcctgttg tgcttaaaag ccctgtaaaa gtatgggtac
    15421 gaaacgatat ggatactggc aaattaacga tcacgccaac acttgacaag aagcaagtca
    15481 ctgacaatga gatccaagca cctatggata aaggtacaaa aatcggtacc gctacgatca
    15541 ccttggcaga cgataaatta ggctatttag aagacaacaa tgcaccttca acagagatca
    15601 ttacggataa aacagtggaa aaagcgaata tattcgtgat tggctggcga cgcgtcgcag
    15661 aatttttcgg caacttgttt taattgaaca ataagaaaaa catctttcca tcggaaagat
    15721 gtttttttgc tttttctagt ttaaataaca gcttttttca tctattttcg ttatactgta
    15781 taagaaaaag gagttgaatt tatgaatcta accagagatc tgatcttttt agttctctat
    15841 cttattgggg tattctctat cgtttattgg cgaaaacaga agaaggatcc ttccttgttc
    15901 ttttacggca gtacgtggac atttacggta atcttaactt attttgtcgt gttggaaatt
    15961 ttttattcga ccttttattt tttgattcca ttgttgttct ttagtatctt tgctttttct
    16021 tatttcacag aaaaacgaag gctgctgaat ggtcttttat tcaatgtttt cctcatcagt
    16081 ttcggcatct acttgtttgt cttgctgtat gaaacgcaaa atattttcct tggcggactg
    16141 atcgccttga tcacgattcc attgctactt gtccttttat ttggtattta tggattgatt
    16201 gtttttcttt tttggaacgg ggtcacagtt ttacgtagag aatcgcattc attagctaat
    16261 cttttaacgt tgatactagc catttttctt acactgtttc ttgtttttga ctttttcttg
    16321 ttaaaatatt tgccgcaatg gataaatgct ttgtttttct gcgttcccct gatcttgatc
    16381 tatctattta tcgtattcta caactttttg actgtctcat tcttgtatca gttcaaccgg
    16441 cctcgataca atcaagactt catcgttgtg ttaggcgctg ggttgatcaa tggagaaaca
    16501 gtttctcctc ttctagccaa gcgaatcaat aaggcaattg ctttttatcg ggctcaaagc
    16561 cgtgctacat taaatccgcc catcttattg atgtctggcg ggcaaggagc agacgaaaaa
    16621 gtaccagagg ccattgcgat gaaacaatat gccatggaac aaggaatccc tgaaagagac
    16681 attcttgtcg aaaccaattc gacaaccact ttggagaaca tgctctattc aaaggaaatc
    16741 atggatcagc aaatgaaagg gccataccgc gcgatcttta gttccaacaa ttaccacatt
    16801 tttagagccg gtctttatgc gtggcaagcc aaattgaaag caaatggtat tggtgcaaaa
    16861 acagctttct actacttgcc taatgcgttt ttaagagaat acatcgccat cttgatgatg
    16921 aacaaaaaaa gacatatgat catttgcgga ttgcttttcg caggctccgc attcttatcg
    16981 ctgattactt tacttttcta aaaaaacaag gtgtgacaaa acatatgtca caccttgttt
    17041 ttttctttca ttctcttaac tattggcaaa aagagtatcc cttttttgac cgctacttgc
    17101 tgagcagaat aaataccgct gttgcctgaa cacataaagc tgatcaagca gatcataaag
    17161 aagtatactg ccgcttgtga accgaacaat tcgatcccca tcacaaagca ggcaatcggt
    17221 gtatttgtcg caccagaaaa tactccgata aagcccaatc ctgcaagaaa aggaacagaa
    17281 acatggagca aaggcgctag tgctgcacca agcgttgcac caatttcaaa caatggtgtc
    17341 acttctcccc cttgatatcc cgcgcctaaa gacaaaactg tgaagaataa cttcccaata
    17401 aaatcataga catgtgcctg tccattaaat gattcttgaa gtaacggaag actcaatccg
    17461 agataacgtt gtgtttgcaa aatcaatgct gctgccacaa ctacagctcc acctatcgta
    17521 tttcgccaaa caggatgagg aatccattgt gcatatcgcg cttttaaaaa gacgatcgaa
    17581 cggctgaata cccaaccaat caaaccaaaa gcaatcccag caacaaaaac tttgaagaac
    17641 agcaacatac tccattcagg aatcacaccc atttggtaat gtagatgaga aacaccatag
    17701 ctttctgtca tgaaatttgc aaacagtgca gcaaagaagc tagggaagat cgcttctgtt
    17761 cggactttcc cgattgccaa cacttctaat ccaaataatg ttccagctaa aggtgtgcca
    17821 aatacagaac taaaacccgc actgattcca ctgataatca agatttcccg ttctgattta
    17881 tccaatcgga acaaacgtgc tgtattatct gctaacgctc cccccatttg gactgctgtt
    17941 ccttcacggc caacagatcc cccaaataaa tgggtagtta tggttccaaa cagtgtaagt
    18001 ggaataagtc gcaacgggat tttttcttct ccaccatttc cttgatcgat cactaagttg
    18061 tttccacgac tagaaagacc gccgtgatac gcatagagat aagcaaaaag cgcaccacta
    18121 actggtagta gataaaggag ccaaggatgt gatagtcgga tatctgtcac aactgtcagg
    18181 ctatttaaga aaaaagcaga caagctcccc atgaatatac cgagaatcgc tgtgatcacc
    18241 agccacttca gcatgtacac taaaatgcgt actggactcg gtaattgttt ttccataaaa
    18301 aattcctcct caataaaaaa acgcctacta aagaaagggg ctgtccccag actttagtag
    18361 gcgtcattag ctatctttcg ttagcattta aaggcgaaca ccatcaccta ttcacttcca
    18421 ttaaaagata gcatattttt ctgtaaaaaa caataaaatt atcgttcttt ctttacacgt
    18481 tcagcaaatt catagaaagg agccaatttc gttaactgtt gattacattg gtcactatca
    18541 tgacaaatat aattcccctt tttcgtatac gtgccatcag aacctgcttt cgttgtagaa
    18601 agaaaaagcg aaacattcga tgttttctga cagattgcac agattccctt atttatcgtt
    18661 ggtgataaag taccactaat cccttttaat ttttcatctt cgtaatagat catgaatttt
    18721 ttctgcgtac ctggatcatt ccagccgatg tacgtatgtt cttgcaagtc gatttcattc
    18781 caagctggtt gtttgagttt tttcactttt cggaataatt tctcgatttg tggtttgcta
    18841 ggctgcataa acggctgaac gatttgcttc agctcctcca atgctcgttc tgcttgcgct
    18901 tttgtcagtt ttacgtctaa aactgtcgtt aaaaatgtat cgatttctgg cagttcgtgt
    18961 tctaatatgt ctcgaatctt ttcagttgct agtgcctgaa ctgtttgtac cgtttttgga
    19021 tcattgacag atgagtacgc atttaataac tgttctactt ctctttttat aaaaaaatat
    19081 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
    19141 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ccggactctt
    19201 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt cttctatgta
    19261 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
    19321 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
    19381 aaagcttgca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag taattttcgt
    19441 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
    19501 ttggttttac ctgttaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
    19561 tacttgcaga catgggctga gccgcttctt tgactgcgca gtatttgtcc acgaatgtca
    19621 caatatatcc ttctttatac ttaggcggtg cgatcgttgc accccacatg tgcttcaaaa
    19681 tgatatctcg ctcaagatcc gacaaatcgg tgattttttc tgcattcttt acagcaattc
    19741 ttggatggat atacgcatgt gtcccctcgt caaatttcgt cgtacgccaa tcatagtaga
    19801 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
    19861 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
    19921 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tgtacataag
    19981 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatctg ccaccttttt
    20041 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
    20101 cgctctagat aaaatagcta aagcttaata gttccttaat cataaagaaa aatgataaag
    20161 aacgatccct gctgcgactc cgacattcaa tgattccgca tttcctttca ttgggatata
    20221 tacgttctga tcagtcaaag acaagatttc ctgacttact ccctgacctt cgtttcccat
    20281 gattatagcg aaatcctctg ttttttctac tttgtctaat gcatgtgctt cttcatttaa
    20341 ttctgtaccg tacaccgtaa accctttgcc tttcattaca gggatcactt ctgccaaatc
    20401 tttttgtaac acaggcaaat gataattgct tccctgcatg cttcgcagca cctttgtact
    20461 ataaatatct gccgttcctt tacctaataa aacaccagcc ataccaaacg catctgccgt
    20521 acggatcatc gttcccacat ttccaggatc ctgtacatta tctagtaaaa gccagcctcc
    20581 tgaaaaatct ggcgtatctt cagatgttaa cgtcatcacc gctaagatcc cttgcggtgt
    20641 aggtagatct gacatagcgg tcatgacctc atctgaaact agaaaagtag aaatctcttt
    20701 gtttacagcc agccagtctc cccattcttt catacctctc tgtgtcacaa aaagttcttt
    20761 tatttctgca tcagacgacg cagcttcttc gatcagatgg aacccttcca aaagatattc
    20821 tgccttttct tctctgtatt tctttttatg tagctttttt aattctttga tttttggatt
    20881 ttttgctgat tggatctctt tcacattcct tcacctcacc tgattatatc atggaatagg
    20941 tgcttgagct tgtttcatcc taaaaaaatc ggtatcattg atttataaaa caggaaagaa
    21001 aggtgacgaa catgcgcaaa gttaaaatga atgtccaagg acgtgtccag ggcgtgggat
    21061 ttcgctatat gaccaagatg gttgctgatc agttaggcgt gaccggaacg gtaaaaaacg
    21121 aagacgacgg ctcagtcaca atcgaagcaa tcggaaatga cgacatcatc caaaaattta
    21181 tcgaagaagt caagaagtct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
    21241 aagatccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtgaaaagg
    21301 tcgcttagtt ttgagtatta aggaaaattt tgtgaaaatg gctttccaca tttttgcata
    21361 aatttgactt aataccgaga aactgacttt tgaacaccgt cgatcaggtt gtttttgagg
    21421 cgctttgcct caagtattaa agataggtag taaaaaagct gatctataac ttgcaataaa
    21481 tttgcttaac ccatattttc cagacagaac aaaaaagttt ttagtttttt atataagaaa
    21541 gaaacacttg gaatcactct tttctttcca agtatttctt tcttgatttt ttgtcttttt
    21601 tcaaaatttt tcgctacgaa aaagaaagaa tatttatatg aaatttctat gaagttgatt
    21661 gaattccatg cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta
    21721 ttctatgaat aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt
    21781 tttatctggc tgtgtgcgga tggattcaaa cggaaaccct gacacttcag gaatcgttta
    21841 tcgagtactc gtccatccta tgggccaagc gattacatat ctagttgaga atttcaattg
    21901 gtcttacggc tgggcagtta ttgcaatgac tgtgatcgtt cgtatcatca tcttgccact
    21961 aggtatcagt caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc
    22021 acaagttgaa gcagcacaac aaaagctaaa agctgcaaca acacgtgaag aacaaatggc
    22081 agcacaagca gaaatgcagc aagtatatcg cgaaaacgga ttgagtatga ccggcggaat
    22141 cggctgtctg cctctattga tccaaatgcc gatcttctct gccttgtatt ttacggctcg
    22201 ctacacagaa ggtatccgtg aatcatcttt ttacggcatc gatctaggct caccaagtat
    22261 ggtgcttgtg gccattgcag gggtcgctta tctgcttcaa ggatatattt caacaatcgg
    22321 tattcctgaa gaacagaaaa agacgatgcg tacgatgctg atcgtctctc ctgctatgat
    22381 cgtcttcatg tcgatcagtg cgccagccgg cgttacactt tactgggtag tcggtggtgt
    22441 gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg aaaccacgct tgaaagcaca
    22501 agtggcagaa gagttgaaac aaaacccgcc aaaacaagtc gtcactccaa gaaaagacgt
    22561 gacagaatca gcaaaaccag ctgacaacaa acaagcaaca aaacaaatca aacaaccaaa
    22621 aaacagtaca aataacaaag gccgcaacgc aggaaagcag cagaagcgga agtagatagt
    22681 aaataag
//
LOCUS       DACESX010000039        22685 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.039, whole genome shotgun sequence.
ACCESSION   DACESX010000039 DACESX010000000
VERSION     DACESX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.039"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(394..2775)
                     /locus_tag="IXF88_001234"
     CDS             complement(394..2775)
                     /locus_tag="IXF88_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1507974.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2765..3412)
                     /gene="recU"
                     /locus_tag="IXF88_001235"
     CDS             complement(2765..3412)
                     /gene="recU"
                     /locus_tag="IXF88_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1507975.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3476..4015
                     /locus_tag="IXF88_001236"
     CDS             3476..4015
                     /locus_tag="IXF88_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1507976.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4105..4524
                     /gene="gpsB"
                     /locus_tag="IXF88_001237"
     CDS             4105..4524
                     /gene="gpsB"
                     /locus_tag="IXF88_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1507977.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4546..4913
                     /gene="rnpB"
                     /locus_tag="IXF88_001238"
     ncRNA           4546..4913
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IXF88_001238"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5122..6288
                     /locus_tag="IXF88_001239"
     CDS             5122..6288
                     /locus_tag="IXF88_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1507978.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6334..7830
                     /locus_tag="IXF88_001240"
     CDS             6334..7830
                     /locus_tag="IXF88_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1507979.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7911..8594
                     /locus_tag="IXF88_001241"
     CDS             7911..8594
                     /locus_tag="IXF88_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1507980.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9239..10429
                     /locus_tag="IXF88_001242"
     CDS             9239..10429
                     /locus_tag="IXF88_001242"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1507981.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10531..11484
                     /locus_tag="IXF88_001243"
     CDS             10531..11484
                     /locus_tag="IXF88_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1507982.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11553..12902
                     /locus_tag="IXF88_001244"
     CDS             11553..12902
                     /locus_tag="IXF88_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1507983.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13085..13408)
                     /locus_tag="IXF88_001245"
     CDS             complement(13085..13408)
                     /locus_tag="IXF88_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507984.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13537..14700
                     /locus_tag="IXF88_001246"
     CDS             13537..14700
                     /locus_tag="IXF88_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507985.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14770..16119
                     /locus_tag="IXF88_001247"
     CDS             14770..16119
                     /locus_tag="IXF88_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1507986.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16164..16595)
                     /locus_tag="IXF88_001248"
     CDS             complement(16164..16595)
                     /locus_tag="IXF88_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1507987.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16983..19625
                     /gene="alaS"
                     /locus_tag="IXF88_001249"
     CDS             16983..19625
                     /gene="alaS"
                     /locus_tag="IXF88_001249"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1507988.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19825..20421
                     /locus_tag="IXF88_001250"
     CDS             19825..20421
                     /locus_tag="IXF88_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1507989.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20543..21691
                     /locus_tag="IXF88_001251"
     CDS             20543..21691
                     /locus_tag="IXF88_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1507990.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21729..22142)
                     /locus_tag="IXF88_001252"
     CDS             complement(21729..22142)
                     /locus_tag="IXF88_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1507991.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22221..22652
                     /locus_tag="IXF88_001253"
     CDS             22221..22652
                     /locus_tag="IXF88_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1507992.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
BASE COUNT         7336 a         3967 c         4752 g         6630 t
ORIGIN      
        1 tcgataaaaa aagatggtgc agacaaatta tgataattaa gcatactatc agataactca
       61 aaatgaagcc ttctttcaaa aaattattca aaaccatata ttgtattttt tatttgataa
      121 taacaatggg attattaaga agtggcacta aataaaagac tagtgtgtat ttagccgatt
      181 taattataaa caagtaaatg gataaagtat atgtaatgat aaaagaaaaa tggattttct
      241 aatttttaag ctaaatgtta gatcacacat gtatatgctg gatagtctta taaaaaatca
      301 tgtaatacag cttggtacat gatttttatt tgtagctgtt ttttacatag acacaaaaaa
      361 gaaaccacgc ttgtagacgt ggtttctttt ttactatctg tttccagaag ctgaggtgct
      421 gctggatggt gtatggtcat ttgcagcatc cgctccgcta gttgctggct cactggaaga
      481 agcaggagga gcagatgaag acggtgtcga gctttcttcg gatgaagaag aaggaaccgt
      541 tgttgaagac gctggctcgc ttgtttctgc tgtcgtgctt tcttttgagg actcagcagt
      601 tgattcgctt tgactagtag agctagagct ttgagtcgtt gtctctgttg ttgagcttcc
      661 aggagtctgg acatagctgg aagatggaat agttgttgat ggtgtaattg cattcgatct
      721 agctgtatat tgatctttgt agtacagttc accgcccaca cgaattaatc cgcttggcat
      781 ctcccagtct gtatttgtca cgttagctga aacatactgc ataagctcgc gataaacatc
      841 agaggctacg tgtgacgatt ctgaagtgac aggtgtcatt tttttattgt aacctgtcca
      901 cacggaaatc gaataatttg gcgtatagcc cgcaaatagg atatctggat acaccccaga
      961 agaaatacca agttttgcat actcgtcatc tgtgtaattg gatgttccgg tcttaccggc
     1021 ttgatataac ccagcgattt gcgcattcgt accagttcct tcagtgatcg tatcttttaa
     1081 aatgtctgtg atcatataag ctgtttctgg agacatggct gtttttccat ctggctcata
     1141 agtttcttct gttccgtctt ggaagacgat tttattaaca tattgaggtt tgtaataagt
     1201 ccctccattg gcgaaagccg cataggctgc tgccattttt aaggaagaag ctccgtattt
     1261 tgtcccatct tgttcttccg tattactaga gatcgcattc gattgatgga tcgtactgta
     1321 ttcgatccca agatttttta agaaagaagc cactttatct gaaccgactt cattgaataa
     1381 tttgactgct ggaacattac gggataagta aagtgcttgg cgtaaagtaa tggtgcccat
     1441 atattgatta tcccagtttc ctacaggagt cgaagtccct tcatagttat acggtgcgtc
     1501 agtaatagtt tttccagtag aatatttcaa atattcaaaa gctggtccgt aatctgtgac
     1561 aggtttcatc gtagaaccaa aatcacggga agtattcact gctaagttgt tgcctaaagt
     1621 tacgtcttct gcgatgtgac gtccgccaat ctgggctttt actttcccgg tattggtgtc
     1681 gataagggtg gaggcaactt gcatctcgtc gtctgggtaa gagacatact gatccgtgtt
     1741 gacgatatca tatagtttct tttgggcgtc cagatccaaa ttcgtataaa tctctaaacc
     1801 gtctgtgtag acattcttat ctgttttttc ttgtacttca ttgatgactt ctttgacata
     1861 attatcgacg atcttagtat tgtcatcgga ttgagttagt tcttgcagac catccgttac
     1921 cggtacatta acagcttgat catattctgt ttgagagatt ttttcgtttt gaagcatcgt
     1981 atacaaaaca gtatctcgtc ttttcttcgc ctgatctgga taaacataag gatcataggc
     2041 ggaaggggct tgaggcatcc cagcaagcaa tgctgtttgc ggtaaagaaa gttctgataa
     2101 tttcttacca aagtacatct cagaagctgt ctccatcccg tacaagccgt ttgacatata
     2161 cactttattt acgtagtagg tcaagatctc ttgtttcgat tttttttgtt ccaatcttac
     2221 agccatccac gcttcttggg ccttgcgttt cagagtctga tcctcggcac tggtggagaa
     2281 gaaggaaagt ttgatcaatt gctgtgtcaa agtacttccg ccttgcagtc cgccagaggt
     2341 aaagttagag agggcagaac cgatgatccg aatagggtca accccgatgt gtttataaaa
     2401 acggcgatct tctaccgaca cgatcgcatc ttctaatgtt ttcggtaatt cattcgcaga
     2461 aattttttca cgcttttctg cgcctaaatc ttcgaatagt tcgccatctt gtgtatatag
     2521 tttggaagat acggtagcgt ctaatttttt atccgtcaat tcgggcgcat cttttgcgta
     2581 ataccagaaa agtcctacac ctgctaaaaa gaggatacag aaaaatgaga gtattcctaa
     2641 aaggatcttg ataaataatc cttttttatg tgttccagag gactttccag aacctttgcc
     2701 agaattcttc ttcacactct ttttaggagt aggctgtttc tgtcgtctag agcttctagt
     2761 ttgttcattt gccattcttg ctttctcctt caatagataa tgttgataaa tagaagtcaa
     2821 ccgcgtccaa ataaggaatc cgcggagaaa taccagtttt gatctctatt cctagttctt
     2881 cgatcaaaga taaaggtagt gatttgcgtc ctttttcctt ttgggcgtcc cagtaagcaa
     2941 tcaaatgaac actattcaaa taaaaacagc gcttcaaaga cgaaaaccat aacaagacaa
     3001 agcagacccc attttgttcg aggcatgctt tcatatgttc gatttgatgg gcatggaagt
     3061 ttttaaaggg aaaagatgtt ttgttcttgg tttcttttgc ttcaaagtcc aagtaaaagc
     3121 ctcgatagac gccattatag tctgtagttg aggcttgggt aaagtaggct tccttgatca
     3181 cagcagcact tctcttagga tagtctactt tcacgatttg gataggggta ggctttttat
     3241 ggataaccgc caacccgcgg gaaagataat atttattact ttcattgatt gcttcttcaa
     3301 agcgcatccc ccgattgccg aattcagtga tctgatttct tttttcttga attttttcga
     3361 cgggagaggc attcttataa taaggttggc catttggata gcgcaatacc atctttaatc
     3421 acactcctag gccacattat agcaaaaaag cggatagaaa gaaaaggaat ctgagatgga
     3481 taagtcaaaa gtaatctatg tgtctgggta tcgaagtttc gaactcggtg tttttaaaga
     3541 aaatgatcct aaaatccaag ttgttaaaaa tgttttaaaa aaagaactcc atcagttagc
     3601 agaagaaggg ctggaatggg tacttgtcag tgggaatttg ggcgtcgaac tatgggctgt
     3661 gcaaatagta gcggaattga aagaagaata tccggaacta tctcttggta ttttgtatcc
     3721 tttttctgat tttggtaata actggaatga agggaaccaa gaaaaaaaac aattagcaga
     3781 acaattagca gattatgtcg aagctgttag ccatcaaccc taccaatcac ctagtcagct
     3841 aaagaatcac actcgtttta tacttgagca tacagtgggc tgtctgctga tctatgatga
     3901 agaattccct ggaaaaacga agtatttttt tgaagatgca cagaattacc aagcagatca
     3961 tccctacgaa attcggctga tcagtatgga cgatcttcaa aattttagtg aataatcgat
     4021 attgtgtttg atttatcaac ttttttccta tacaatgaaa aaggtagaaa tgttattacg
     4081 taaatgaaaa ctagaggtgt aaatatggcg aatttggttt atagtcctaa agacatttta
     4141 caacaagaat tcaaaacaaa aatgcgcggc tatgatcccg ttgaagttga tgaattctta
     4201 gacaatatca tcaaagatta tgaaacttac agcaaagaac tccttgcttt acaagaagaa
     4261 aacgatcgat tgagtgcaaa agtagctcaa ttatctaaaa ctcaaggagc agctcaaaca
     4321 cgcgtgcaac aaacagaggc accaaagagc gcagcagtga ccaattttga tatcttaaaa
     4381 cgcttgtcaa atcttgaacg tgaagtattt ggtaaaaagc ttgatcaaca agcaagtgca
     4441 gtcaaaccag cacaacctaa tccaaataac tacacaaatg cagacacaag cttagatgat
     4501 aatgaaaaaa cacgtcaatt ttaatttttg attaatgaat ttgcggtttt cgggtaatcg
     4561 cggtctgact tgatcaggct gaggaaagtc catgctcgca caggctgtga tgcctgtagt
     4621 gttcgtgctt agcgaaacca taagctaagg tactctttgg agtaacggca ggaaaacaag
     4681 ctaaggcttc ggctatgctt aagtatcctt gaaagtgcca cagtgacgaa gcggctgggg
     4741 aaacccatcc ggtggaacgc ggtaaacccc tcgagcgagc aacccaaaca atggtagggg
     4801 cgctttttgt ctggaagaag aacaggacaa aaaggcagta taactgcaga tagatggtta
     4861 ccgctttgtt ttttctccct ggaaaagcaa agtacagaac atggcttata gaaaaccgca
     4921 aacatcaggg aattaggtcg tgacaaaagt aatgcgttat aagaaacaag aaaaacattt
     4981 gaaagctatt tttcaatact tcagatgaat cgttcttatt acaaaacaaa cttttgtatc
     5041 accggttatt cggaatttga gtaaaagtgt ggggtaggac tgatgtccca cctccttttt
     5101 ttataaataa ggagattgtg tatggaaaaa aacaggaagt tcaacctcgt cgcaactgcg
     5161 gcaagcgggc tagaagcact ggttggtaaa gaattaagag atcttggaat cgaatgccaa
     5221 gttgagaacg gacgtgcacg ttttgaagga acaatggaaa cgatcgctac agctaattta
     5281 tggttacgta cagcagatcg agtaaaaatc gtggttgggg aattcgatgc gtatacgttt
     5341 gatgaattgt ttgaaaacgt aaaagcattg ccttgggaag attttcttcc actagatgca
     5401 gcttttcctg tagctggaaa atcgatcaag tcgaaactat atagcacacc tgattgccaa
     5461 gcgatcacaa aaaaagcaat cgttgaacgt ctccgtacct attatcaccg tccagcttct
     5521 gtaccactga cggaaactgg cgcacatttt cagttggaag tagctttgct gaaagaccat
     5581 gtgatggtaa ctctggatac gacaggacca agtttattca aacgtggtta tcgcttagaa
     5641 aaaggtggag caccattgaa agaaaacatg gcagcagcac ttattatgtt gacaaattgg
     5701 cgaaaagacc gtccatttta tgatcctgta tgtggttcag gtacgctttg tattgaagca
     5761 gccttaatag gccataatat cgcaccagga ttcaatcgtg agtttgcttg cgaatcttgg
     5821 gattggttca gtcaagaaat catggaaaat gttcgatcag cagccgaaga aaaagcagat
     5881 tatgatattg aattagatat tactggttca gatatcaatg gacggatgat tgaaattgcc
     5941 aaagccaatg cagaagaaat cggattaggc tcttcgatta cctttaagca acaagcagtc
     6001 aaagacttca aaacggataa agaatacggg gtcatcgtgg ctaatccgcc ttacggagaa
     6061 cgtctaggag aagaagaaac tgttcggaaa ttatataaag aaatgggcga tgtattccgt
     6121 ccattaaaaa catggagtaa atatatcttg actagcgact tagcgtttga ggaattttac
     6181 ggacaaaaag caaccaaaaa aagaaaattg tacaatggtg cattgcgtac ggatctattc
     6241 cagtattggg gaactcgtcc gcctagaaaa aaagagaaag acgaataatc ttactaattg
     6301 attcattcaa ttggatgttt ggaggaatag ggaatgaaag aaaaagagtt tttacaagaa
     6361 gtaaaagaaa tcaatcttct acaacaagct ttaggaatcc tcgattggga tacacaaaca
     6421 ggtatgcctg aaaaagctag tgcataccga agtgaagtag acagctatct ttacagcatt
     6481 tatttttcaa aaaaaatcgg tccaaaaatc aaagaagcca ttcgctattt ttctgaacat
     6541 ccggaagaat tgtcggaagt tggaactgcc gtttttgcca aagtaaaaga agagtatgat
     6601 ttggaacatc gcgtaccgga agatcttatg agaaaatata cagctgccgt ttcttcagca
     6661 cacggacaat ggcaaaaagc tagagaaaca caacaatttt ctgattttca acacgcattg
     6721 aatcaaaata ttgaattaac aaaacagttg ataccttact ggagaaaaga tgaaaagacg
     6781 aattatgatg ttttgctgaa tcaatacgaa cccgggatga ctgtcgaaat ccttgataag
     6841 gtatttgatc agcttaaaga aggaattctt tcgatccgcc gtacgctcca agaaaaaggg
     6901 actgaacctg aaacagactt tttaaatcgg agaatgacga aaaaacaaca gaagagattt
     6961 gtagtgaaag taatcgaaca acttggttat gattttagtc gtggtcgttt agatgacact
     7021 gtccacccat ttatgactgg aattaatccg aatgatgcac gaatcacgac acgatggaac
     7081 gaaacagatt tcaaaatggc tgtttttgga gtgatccacg aggctggtca tggtatgtat
     7141 gaacaagaca tcgatgataa atatgcgtat acaccggttt atgaaggtac atcaatgggt
     7201 atccatgaat cacaatcgtt gtttaacgag atcattattg gaagtagtcg cagcttctgg
     7261 aaaaagcaat atcctttctt tcaagaatgt gcagaaggaa cattcgatga tattcctttt
     7321 gacaaatttt atcgtttatt gaaccataca aaatcaagtt tggttcgtat cgaggcagac
     7381 agtttgactt accctctcca tatcatcatt cgttatgaaa tcgaaaaaat gattttcaat
     7441 gaaggggtag atgttgctga actacctgaa atatggaatc aaaaatatga agagtatctg
     7501 ggaatacgac caacaaatga tgtggaaggc atcctacaag acgttcattg gtcaggtgca
     7561 agctttggtt attttccctc ttatgcatta gggtacatgt atgcggcaca attacttcat
     7621 tctttgcagc aagaaataga tgtggaaaat gttctagcgt cagacgatta ttctccaatc
     7681 aaaaactgga tgtcagagca tatccatcaa tatggtgcat ctagaaaacc aaatcaatta
     7741 atcatggatg caacacatga accgcttaat ccgcaatacc tgatcgacta tatgaaagac
     7801 ctgtatttta atgtatatca ggtaaaataa aatacaattt acaaagatag ctgaaattca
     7861 tctaatctat agatgttttc agctatttac ttaagaaaga agtggaacga atggataaat
     7921 tacaagtgat cgctgattat agtatggacc aattagcgca tgatcaaaca ggacatggga
     7981 gcgatcatac caaaagagtt gtcaagttag ctgaaaaaat tttggagacg gaaccgcagg
     8041 cagaccgctt tgtgacgctt gcagcagctt acctacatga tacgattgat gataaagtcg
     8101 ttaaaaatga gaacgaggca aagcaacagc ttcgtgtatt ccttcgctct ttgccaatag
     8161 tagaggaaca gatctcgatg attttttcca ttatcgaaaa tatgtccttt tctaaaaatc
     8221 tctctgaagc ggccgaacta tctctggaag gtaagatcgt tcaagacgct gatcgtatcg
     8281 aagcattagg agcaatcggc attttgcgta ctgcttattt cggcggtgga catgggcacc
     8341 cgatttttga tcctgaactg tatccgcaaa cattcaagga caaagaaaat taccgaaaag
     8401 gcacgacagt gatcaatcat ttttatgaaa aactgttctt gctgtctgat aaaatgaata
     8461 ctgattacgg atatgaagaa gccaaacgaa gagaaacctt catgcgtgaa ttcttagaag
     8521 aattttttct ggaatggtct attgacgatg aaaattttac tccagacaat tggatcaaaa
     8581 ataacaaaaa atgatggagg ttgtgacaga agtggtcagc tccaagatat ttcggatcat
     8641 tttccgtaaa agttacttct ggaacactgt ttaccaagag gtcgtgagcc tgccgttcag
     8701 ctccgagtca caaggaacaa tttcaaaaaa tagcttctcg tgtttgatga atattgtaac
     8761 ttgtgattcg gaattggcag gagaacaccg tttattcgga agaataggac tgtgacaaga
     8821 cttttgtcac agtccttttt tcgaattcaa caagtaaaac ttttaagaaa tattcagaga
     8881 gtaaaattaa aaattgctga taaacgaaga tggaaatttt atgtattttt attggtctat
     8941 gcaataaata aattaaatat tcagaataca attgatttta acgaaagggc ttctatcaat
     9001 ggttgtgttg tatttatctg cccaggtaga gtaaaataga tcattatcta aaaggtaatt
     9061 gttcatacta tataagtcat ttgaaagtaa aaagatactg taaataaaaa atgtggttaa
     9121 ataattctca taatcagtgt aagcgttatc aaaaatatag catacttgct ttgtagatga
     9181 atgacacgat atatgtttgg tatgttctgc aagacagatg aatatggagg tggagacaat
     9241 gtcttccaaa gaactaacaa tgtatttaga gaaagaattg gatcaactga aagaaaaggg
     9301 gttatacaat acgatcgaca tactagaaag tgaaaatggt gcttgtatca tcgtagacgg
     9361 aaaaaagatg atcaatctag catccaacaa ttatcttgga tttgccaatc gcgaagaatt
     9421 gaaaaaagca tgtattgaag caaccgaaac ttatggtgta ggagcaggag cagtccgaac
     9481 gatcaatggt tcgctgaaaa tccatcagca attagaagaa aaaatcgcag aattcaaagg
     9541 gacagaagca gcaatcgcct ttcaatctgg attcaactgc aatatgggcg ctatctctgc
     9601 agtaatgaca aaagaagatg ctatcttatc ggatgaatta aaccatgcat ctattattga
     9661 cggttgccgc ttatccggag caaaaatcat ccgaattaaa catcaagata tgaaagacct
     9721 tgagaaaaaa gcaaaggaag cgatagaaag taagaaatat aaaaaaatca tgtatatcac
     9781 agatggtgtt ttctccatgg atggagacat tgcacgattg ccggaaatta ttccgattgt
     9841 tgaaaaatat ggactgatca cctacgtcga tgatgcgcac ggctcaggcg taactggaaa
     9901 aggtgcagga accgtcaagc attttggatt aagcgacaaa atcgatatgc aaatgggcac
     9961 gttatcgaaa gctataggtg ttgttggggg ttatgtagct ggatcgaaaa cactaatcga
    10021 ctggttgaaa gctagaagtc ggccattcct attttctact tcgctaactc caggtgcagc
    10081 tgcagctgca ttggcttcga ttactttgat gcaagaacat ccggaacttg tcgaaaaagt
    10141 ttgggaaaat gctaattatt ttaaagaaga attgaaaaaa gtaggctata acatcggtat
    10201 gtctgaaaca cctattactc cagtcatttt gggagacgaa aaagtgactc agactttctc
    10261 taagaagctg atcgaacatg ggatttatgc taaaccaatc gtttatccaa ctgtgcctct
    10321 tgggacaggc aggatcagaa atatgccgac tgcggaacat acgagggaaa tgcttgatga
    10381 agcagtagca gtttatcaaa aaataggaaa agaaatggaa attatttaac taaatttagt
    10441 tttttcgtct atttaaaatt agagaacatg ttcacgcata tattgttaat catctgaaga
    10501 tagtagtaaa aaagatcagg gaggatcatc atgaaaaaag tattggtgac aggatgttta
    10561 ggacaaattg gttcagagtt gatcacacgg ctacgtcaag aactaggcag tgaaaatgtc
    10621 attgcgacag atatacgcat gcctgaagtc attgagaaaa acgatatctt tgagatccta
    10681 gatgtaatgg attacgaaaa aatgcgcagc atagcagaac aatatcaagt ggacacattg
    10741 attcatttgg ctgctctttt gtcagctgtt gcagaagcaa aaccacaatt agcctggaat
    10801 ttgaacgtga ctgggctggt caatgcattg gaagtcgcta gagaattcca gctgaaattt
    10861 tttacgccaa gttcaatcgg cgcgtttggt ccaaatactc caaaagatca taccccacaa
    10921 gatactcttc agcgtcctac aaccatgtat ggggtaacga aggtagctgg agaactacta
    10981 tgtgattatt attatgtgaa atatggcgta gatactcgtg gtgtccgttt ccctggattg
    11041 atttcttatg caaccttgcc aggaggcggt acgacagact acgcagtcga tatttattat
    11101 tcagcaatca aagaaggaaa gtacactagt ttcattgatc aaggtacatc aatggatatg
    11161 atgtatatgc cagatgcgat cgatgccatt ataaaactca tgaatgcaga tcctgatcga
    11221 ttggttcatc gaaatgcctt caatatatcg gcgatgtctt ttgacccgga agagatcaaa
    11281 gcatctattc aaaaaatcat accagatttt gaaatggatt atgatgtgga tcctgtacgt
    11341 cagtcaatag cagaatcttg gccaaattca ctagatgttt cttgtgcgca agaggaatgg
    11401 ggcttttcgc cgaaatacga cttagatgtg atgacaaagg atatgttgga aaaactgaaa
    11461 gaaaaattgg tagagacggt ataaaacaag aaccacatcc aaaaagtttt ttatactttt
    11521 gggattggct atggtatgaa aggggacaag ggatggaaga atatcaagcc acgccagtaa
    11581 aagaagtaaa aatagaaaat gaattaaaac gtacgatggg attttttact gctttatcga
    11641 ctgttatggg cacagtgatc ggtgcagggg tattttttaa agccgcaagc gtagcagaag
    11701 ttacaggatc cgccagtttg catatgtttt cttggtttct cggtggtatg atttctgtct
    11761 gtgcaggact aacaggagct gaactggcag ctgctattcc agaaacagga gggatgatta
    11821 aatatatcga acggatttac ggaaatacag cagctttttt attgggctgg gcgcaagtcg
    11881 tgatttattt tccagcaaat gtagcagcct tgtcgatcat tttcggtaca cagttcgtca
    11941 atctatttgg tctttcccag tcaatgattg ttcccgtagc tgttacagct gctgtatcga
    12001 tcctgttgat caatttttta ggttctaaag ctggaggggc ttttcagtcg attacgcttg
    12061 tctgcaagct tattcctttg tttgtgatcg tcattttcgg tttgttccga caagaaggag
    12121 tagacttcca actgtttccg atccaagcag gagaaaatct ttcatttttc tcagccttgg
    12181 gagcgggctt gctagcaacg atgttcgcat acgacggttg gatacacgta ggaaatattt
    12241 cgggagaatt gaaaaagccg gcgaaagatt tgccaaaagc aatttcttta ggaatcatcg
    12301 gaatcatgat cgtttattta ttagtaaatg cggtgttctt aagaaccgca tctatcgacg
    12361 gagtcgcagg aaacagcaat gcggcaagtg acgttgcaaa aatgattttt ggcggcttcg
    12421 gtggaagact ggtcacagtt ggtatcttga tttctgttta tggaaccatc aatggctata
    12481 cccttactgg aatgcgtctc ccttatgtta ttgcaaaaga aaacaacctg ccttttagca
    12541 agctctttgc taaacttcat gataaaacta aagttcctgt agccgcaggt atcttagagc
    12601 tagtcatcgc tattggcatg atgatgatcg gtggttttga tacactaacg gatatgctga
    12661 tctttgtcat ttggattttt tatacgatgg tctttgttgg tgtcattctt cttcgtaaaa
    12721 aagaacctga tttgtttcga ccatacaaag ttccaatgta tccgtttatt ccactagtag
    12781 caatcattgg agggactttt atcgtatcaa gcacgcttat cactcagacc tttttagctt
    12841 cgatggggat agtattgaca cttgctggta tcccgattta tctctattta aaacgtagat
    12901 agctaaaaca ttcgaatttt attagaactg cacttctctg ccaaacaaaa tattttattt
    12961 aaatgaggag tacgtggaag aaatgtcaaa agtactcgtt aattttgatc acgtaaatgt
    13021 cgttttgtct acgatgaaat agcaaagtaa aagacagaaa tcgggctcaa ccgatttctg
    13081 tcttttactt tgatttgcgt aactgaacta ttcggcgtac gccgcgataa atatttaata
    13141 aaaccaagca accagaaatg aaaatgggaa gccaaatcag atagacaggg tagccaagca
    13201 ctttagacaa agcatgtgga gtcattttat ccatttcaag aagtgcttgc tgccaagtct
    13261 gaagattgaa gactagcatg aaacccgaaa taatcaggag aataaatcct aaataatgaa
    13321 ttgtagttct ttttcttaca taagcgatcg tagcataaag gaacagtaaa aaaataggta
    13381 cgccaaaaac aaagtacata ttcagcatct cgacaccccc tttgtgttta tcttacttta
    13441 ctttgaaaga aatgagaaat aatagcctcg ttacttgctt gaaacagcaa aggactgcta
    13501 agataagaga gatacaaaga atcaaggagg agtcatatgg caagttatat cgaaccgatt
    13561 cgttttgcgc tgattgtttt tccattttta gccttagcga tctcatctgt tttttttatt
    13621 tatgaatatc gaaagtatgg aacatttcta gtaacaaggg cagttatttt gtattctttc
    13681 gttttttatt tattgtgtgc gtattttttg gtgatcttac cacttccggc aaaggagacg
    13741 gttgcccaaa tgacagggcc aaagatggag ctgcatttat ttgcttcatg gcaacatttc
    13801 agagacaaca cggtattggt cttgtctgaa cctagtacct acctgccggc aatgaagcag
    13861 agtgtatttt tggaaccagc ttttaatgtt ttattactca taccatttgg catttattta
    13921 agatattatt tccggctttc ttttatgaaa acgatgattg ctagtttctg cctttctcta
    13981 ttttttgaat tgacgcagct aagtggactt tattttatct acccaagacc ataccgctta
    14041 tttgatgtga atgatttagt gacaaataca ttgggtggaa tcattggttt tgtcgttacg
    14101 ccgttattta catttatgct tcctacaaga agacgaatgg atgaactttc ttatgaaaaa
    14161 ggaaagactg tctcattgac tcgacgcttg gtcgcttatt tgatcgactg gacattttta
    14221 tctcttatta atttggtagc ggcgattttc tttaaaattg cgttcgattc aggggcagtt
    14281 acttctcata tctggtggct tttattagaa gtaggggtat actttatgat tgtaccgttt
    14341 ataaccaatg gatggacact tggcaaaaaa attgtgcgta tccgtttggt ggaagaagac
    14401 cgcgaacgaa tttctttaaa agcattgatg atccgctacg ggtatctgta ctttcttttt
    14461 tatggtttaa gctggttggg gcagttgctt cagcaaatca tgaattcgac aacgaatacg
    14521 gtgttgcaga tgagtgcagt attcatttct ctcggtgtct tgttactaca aggtattttt
    14581 attttaaata tcttgctatc atttttgaga aaaaaacgga ttctgtttta tgaaaaagct
    14641 agtcacacat atactgttag cacgattggc caaacaaagg atgatgattc tcgaaattag
    14701 ctgagcaaaa acgctttgtc tgtgttataa tgccaaagtc gtgataattt aaagagaaga
    14761 ggtgcacgaa tgaataattt tgatatattc cgcttcaaac cgtttatcaa tgaagcgtta
    14821 accgcaaagg gcttcaaaca gcccacagag gtccaggaac gtttgattcc tttaattgca
    14881 aaaggaaaaa atatcattgg gcaatctcaa actggtacag gaaaaacaca tacgtttttg
    14941 ttgcctttgt ttgaaaaaat caatgcagaa aaagaggaag tacaagttgt tattacagct
    15001 ccaagtaggg aactagctgc acagatctat gaagcagcta cacaaatcgc taaattttct
    15061 acaccggaga tccgtgtctc gaattttgtc ggcggtacag acaaacagcg tcaattaaat
    15121 cgtctgcatc accaacagcc acaggttgta gtaggcacgc ctggtagaat attagacatg
    15181 atgaatgaac aagcgctgaa gattcacacc gcatttgcat ttgttgtcga tgaagcggat
    15241 atgacattgg atatgggttt tttaaatgaa gtagaccaaa ttgcaagtcg attaccagaa
    15301 aagctgcagt ttttagtttt ttctgccact attccagaaa aacttcgtcc attcttacga
    15361 aaatacatgg aaaatccaat cattgaagaa atcaaaccaa aagcagttat ttctgaagac
    15421 attgaaaatt ggctgatttc aacaaaaggt aaagatgaga atcaaatgat ttatcaactg
    15481 ctgacaattg gtcatcctta tttagcaatc gtatttgcca atacaaaaca gcatgtggat
    15541 gaaattgccg attacttgaa aaaccaaggc ttgaaagtag caaagattca tggagacatc
    15601 accccaagag agagaaaacg agtgatgcga caagtacaga atctggagta tcaatatgtt
    15661 gtagcgactg atttagctgc tagagggatc gatatcgaag gtgtctctca tgttattaat
    15721 gcagaaattc cagaagatct agacttcttt atccatcgtg tcggacgtac tggacgtaat
    15781 ggcttaaaag gaacggccat tacgttatat tcgccaaatg atgagcaagc cattgatgcg
    15841 atagaaaaat tgggcgtctc atttgaacca aaagaaatca aaaacgggga aatcgtcaaa
    15901 acatacgatc gtaatagacg tgccaaacga gaaaaatcaa aagatgtatt agatcccacg
    15961 ctgattggtc ttgtgaagaa gaaaaagaaa aagatcaagc ctggttataa gaaaaaaatc
    16021 gattgggcaa ttgcagaaaa aaataaaaaa gaacgcaaaa tcgaacgcag acagcaaagc
    16081 cgtactgctc gtaaaaataa gaaaaattca cataaataaa aaacaagagg ttgtgacaaa
    16141 aggcgtgtca caatctcttg tttttaagct agactttttt tcattccgat acaaagtact
    16201 tcatcttcaa taaaaggttc tgtgcaagta tgataccctt gtttttcata aaaatctaca
    16261 gccgtcattt cagctactac ataactagtc tgacaacctt ctgaaaccgc ccgtttttca
    16321 gcagcttcaa gcaactgacc gcctaaccct tttcctcggt attcttttgc tacgcaaaat
    16381 cgttccggct tgaagcaagt aggtgttttc ttttcataac gcaaagtagc tactggtctt
    16441 ttttgatcaa gaaatagata ataggtttgt tgcgggatat cttttccgtc gaattctagt
    16501 tctgcaggaa tcccttgttc taatacaaaa acttgatatc tcaaaaagca gctagctgcg
    16561 cgtatccatt catccgctcc aactattttt ttcatcatat tcctccttgt catgaatagc
    16621 agagatgtat tgattccttt attcttatcg taccaaaaat cgtggagtat gaccaaaagg
    16681 aaatgaaaat aaaaattgag atattgacag tgatcaatag aatgcgtata ctaattaagt
    16741 acgaaaagga tatgatttgt tttttaagac tcacagaaaa ttctttcttg gctgaaaaaa
    16801 gaatagtcac taatcaaatt gctccctttt tctattttgg tagaaatacc gacgcagctc
    16861 agcgttatcg agtttaagag gtaatgatta cacatcattg caatcaaggt ggtaccgcga
    16921 gttttcgtcc ttggctgtga tggtgttttt ttgtatttaa tttatagaaa gcaggtaatg
    16981 acatgaaaca attaagtagt gcacaagttc gacaaatgtt tcttgatttc ttccaatcta
    17041 aaggacatac gattgaacct agtgcgtctt tgatccccgt gaacgatcca accttattgt
    17101 ggatcaactc tggtgttgca acattgaaaa aatattttga tggttcagtt gtgccagaaa
    17161 atccaagaat cacaaatgcc caaaaatcta ttcgaacaaa tgatatcgaa aatgtcggta
    17221 aaacagcacg gcaccatacg atgtttgaga tgctgggtaa tttttcgatt ggtgactatt
    17281 tcaaaaatga agccatccac tgggcatggg aatttttgac tgatccaaaa tggatggcat
    17341 tcgatccaga aaaactttat gtaacagttt atccaaaaga tacagaagca aaacgcatct
    17401 ggcatgaaga agttggcctg ccactagatc acattattga tgtagaagac aacttctggg
    17461 atatcggtgc aggcccatgt ggtccggata cggaaatttt ctatgaccgt ggagaagctt
    17521 ataacgattt agcagaagac gatcctgaaa actatccagg aggagaaaat gaacgttatt
    17581 tagaaatctg gaacttagta ttttcagaat tcaaccataa gcctgataac acatttgaac
    17641 cacttcctca taaaaacatt gatacgggga tgggattgga acgcatggtc tcaatcgttc
    17701 aagatgcgtc aacgaacttt gagacagact tgtttatgcc tattatccac gccgtagaaa
    17761 agctaagcaa acaagtaaaa tatggtgaaa acccagtaac agatatttct tacaaagtaa
    17821 tcgcagatca tattcgcgcc ctttcatttg ctattggcga cggtgcattg ccatcgaatg
    17881 aagggagagg gtatgtctta cgccgtttgc ttcgtcgtgc tgtcatgcat gggaaaaaat
    17941 taggtattga acaagcattc ttatatcgtt tagtccctgt tgttggggaa atcatggtaa
    18001 gttattatcc tgaagtactt caacaaaaag aatttattga aaaagttatc cgtacagaag
    18061 aagaacgttt caatgaaaca atcaatgaag gtttggaaat cttaaatcaa gtgatccaag
    18121 aagtaaaaaa cgaaaacggg acgactctag caggaaaaga catcttcaaa ctatacgaca
    18181 catatggctt cccggtagaa ttaacggaag aggtggcaga agatgaagga ctgaaagttg
    18241 atcatgctgg ttttgaaaaa gaaatggatg cacaacgcga acgtgctcgc tctgcccgca
    18301 gtactgaaaa atcaatgggg gtacaatctg ctttattgac agatatcaaa gtagaaagta
    18361 aattcattgg atacacagaa caagaatcag atagtcaatt attggtcatc gttaaaggtg
    18421 agtctctttt gaatgaagtc gcagaaggtg aagctcaaat gatctttgat caaacacctt
    18481 tctatgcaga aatgggtggg caagtagcgg atcaaggatg gatcatgaat gaagtaggcg
    18541 aaatcgtagc agttgttaca gatgttcaaa aagcgcctaa tggtcaattt atgcacaccg
    18601 tagaagtaaa agcaccaatg agtgaaggac aaacctatca tctattgatc gatcaatcga
    18661 tgcgcaatcg gatcatcaaa aaccatacag caacccatct actgcacaag gcattgaaag
    18721 aagtacttgg cgagcatgca aatcaagctg gttctttagt cgctcctgga catttacgat
    18781 tcgactttac ccactttggt caaatcactt cagatgaatt agctgaaatg gaaagaatcg
    18841 tcaacgaaaa aatctgggaa gctatcccag ttgaaacaat tgaaacagat atcgatacag
    18901 ccaaaaacat gggtgcaatg gctttgtttg gtgaaaaata cggacatgac gttcgagtag
    18961 taaatattgg cggctggtca atcgaactgt gcggaggcac acatgtagca aatacagaaa
    19021 acatcggaat cttcaaaatc gtttctgaat caggaattgg tgcaggcgtg cgacgaatcg
    19081 aggctgtaac aagtaaagaa gcgtacgaac tacttgaaga agaagaaaaa caactaaaac
    19141 aagtagcaac aattgttaaa tcacctcaat tgaaagaagt tgtttcaaaa gcggaacaat
    19201 tgcaacagca agtaagagag ctacaaaagg aaaatgaaca attagctagc aagttagcga
    19261 atcaacaagc tggtgatgta ttcaaagatc cagaaacgat caatggaaaa acaatcattg
    19321 cagcacaagt aaaagtgaaa gatatgaatc agctgcgtca attagctgac caatggaaac
    19381 aaaaaaatat ttcagatatt cttgttttag caaacgaaca agaaggaaaa gtcagtctgc
    19441 tagcggcaat gacgaaagaa tcaaatgaag ctggactaaa agctggcgac ttgattaaag
    19501 caatcgcacc gaaagtaggc ggtggcggtg gtggacgtcc agacatggca caagctggcg
    19561 ggaaaaatcc tgctggtatt ccagatgcac ttaaagctgc gaaagaatgg gttgcgcaac
    19621 aatagggcgt ttacgtttga cagaaaaaca aaagttcaaa cattgaacga aacatgatta
    19681 tgggggcgaa gacaaacaag tcttgccctc atactttttt caattggatg gcgcatctaa
    19741 tcaaatggtc ggattcggat ggtctttagt tgacgaaagt gacagaaatt tttacgatta
    19801 gtataaataa aaaggggtga aaaaatgaat agtagaaata agacctttcg actagtctta
    19861 cgtgcgatct tgttagcaat catcatcgta caagcgatgg ttccttggct ggggtttatt
    19921 ccattaggat tcattagttt aacaattatc catatcacag taattattgc tgcagtagta
    19981 ttaggcccaa aagatggcat ggtgattggc ttgttttggg gaatcgctac aattgttcgt
    20041 gcctatgcca tgccaacaac gccatttgat acattagtgt tcacgaatcc gattatctcg
    20101 gtagttccca gagtacttgt tggcttagta gcaggcttgg ttttccactg gatttatcaa
    20161 aaaaataaat caataacgat cagctcagcc tttgcagggg tactgggttc attggtcaat
    20221 acagtcttag tgttaggatt catgggatta ttttatacag gtgcaacggc agatgcctat
    20281 ggtgtagact ctgctctttt atttaaaacc ttagcagggg ttgcagcaat caatgggata
    20341 ccagaagcaa taggtgcggg aatcataaca ccattactag caaaagcgtt atttgcagca
    20401 acaccgttga aaccagaata atgaccatct tttaatagta ataggacgca cgatagatag
    20461 aaaaaggaag agaggagagg aaaacaatcg tcctctccat ctttttacct gagcaacaag
    20521 caaactagaa ggggtaaaaa acttggctaa gaaacaagaa gaaaaaaaga aacaaatacc
    20581 gcaacagcgg acaaaaaaag gagtactagc gggtatctta ggattggtta cgaatctgat
    20641 cttgtttgtc tctaagctaa tgatcgggct tttttcagga agtgtctcca ttatggctga
    20701 tgcagtgaac agtctatcag atactgcttc atccatattg actttgatcg gatttaaaat
    20761 tgctgcaaag ccagcagatc aggaacatcc ttatggacac gaacgcttcg aatatatcag
    20821 tgggttattt gtttcgatca tcattactta tgtaggattt caatttttag agtcttctgc
    20881 tgaaaaaatc atccatccag aaaatgtctc tttaacaccc atcgtctttt ttgtcctaat
    20941 catttccatc tttttgaaat ttgcacaagg caaaatgtat caggttattg cgcaatccat
    21001 tgattctgaa acattgagag ctacagcaac agatagttat aatgatgtct ttactacact
    21061 ggctgtattg ctttcagcag caatcgaatg ggtaactgga tggcgtattg atggatatat
    21121 aggttttgct ttagcattat atatccttta tagtggattg acgatgcttc gaaattttgt
    21181 atacgaacta ttaggaagtc gcccaacgcc agcagagatt gaagaaatgg aagagcattt
    21241 aaatgcttgc gatgatatac ttggctatca tgatttattg gtgcataact atggacccaa
    21301 taaacgcttt gcatcagtcc acatcgaagt agatgaatca atgaatttga atgtggctca
    21361 taagattatt gatctgatag aaaaagactt taaaaagtct ttggatgtgg aacttgtctg
    21421 tcacttggat ccaatgcctg ttgataacga aaaatactat aatatcttgc ggcagttaaa
    21481 gcaaattctt cttgaaacag gtacagggct taatattcat gattttagag taattcatca
    21541 aggaaaaacg ctgcaatttg atgtagtggt ccctgagaaa ttccagtttg ttgatacaga
    21601 actagaaaga atcttacgta gtcaaataga agaaaaaatc ggagattacc aattggaaat
    21661 tacgtttgat cacaattatc ttttatatta aatgagaaac tataaaaaga ctccttaaag
    21721 tttttaattt aaagagtctt tttttctttt aattgtttat ggagagcctg acgtgcaaga
    21781 ttatctgctc ctttattttt gttttcgggc aaccattgca ggatcaataa agaaaattcg
    21841 gatagaagtt cttggattct ttgaaggtag ggaaggaaga gctcgttctt cgttgcgttt
    21901 ttgtcgaccg tagaaactag tgttttgctg tctgaaaaac agaaaatcgt ttcattctgt
    21961 aagtcgtttt tctttagaaa ttctaaagtt tttaaaaaga cttcgaactc tgcttgatgg
    22021 tttgtcccta tagaaagcgg gatagatagc tgttcttgtt taccttgata gacaatcaag
    22081 attccacctc cactaggacc agggttgcct tttgtagagg catcaatgta tacttttaac
    22141 ataaaaagag attctccttt ttgttcaaac taatgttatg gtaagattat atcattactg
    22201 aatcggtgag aggttagaga atgaaaaaaa atattcgatg gcagcctgaa ctggcacata
    22261 cgatcatcta ttggtcattg accttgatca ttctgttttt tagtctgatt ctttctttag
    22321 aaaacacagg tccttattgg aaaagcaatt ttgttttagg tatttttctt gtttttctat
    22381 ttctaggttt gcggcgttct tttattttaa aatcaaactc tgtgattata cgttatgctg
    22441 ctttttggaa agaccgagaa atatctgtcg gacagattcg atctttgacg ccaactaata
    22501 ctggggtgca aatgatgttg caagatgaga aagccccatc tgttttcatg atgcgaaaaa
    22561 aagatcaagt agtccttttt gatcattttg aaaaaaacgg acccaaatca gaatcaattc
    22621 cttatcctta tacgaaaaaa agaaaaagct aattttaaaa atcagaacct aaaaaaacga
    22681 atttt
//
LOCUS       DACESX010000040        22447 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.040, whole genome shotgun sequence.
ACCESSION   DACESX010000040 DACESX010000000
VERSION     DACESX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.040"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            25..696
                     /locus_tag="IXF88_001254"
     CDS             25..696
                     /locus_tag="IXF88_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1507993.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IXF88_001255"
     CDS             755..1195
                     /locus_tag="IXF88_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1507994.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IXF88_001256"
     CDS             1207..1875
                     /locus_tag="IXF88_001256"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1507995.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IXF88_001257"
     CDS             1939..2562
                     /locus_tag="IXF88_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1507996.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IXF88_001258"
     CDS             2618..3004
                     /locus_tag="IXF88_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1507997.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IXF88_001259"
     CDS             3217..4608
                     /locus_tag="IXF88_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1507998.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="IXF88_001260"
     CDS             5219..5587
                     /locus_tag="IXF88_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1507999.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="IXF88_001261"
     CDS             5767..6708
                     /locus_tag="IXF88_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1508000.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6777..7508
                     /locus_tag="IXF88_001262"
     CDS             6777..7508
                     /locus_tag="IXF88_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1508001.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="IXF88_001263"
     CDS             7652..9832
                     /locus_tag="IXF88_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1508002.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="IXF88_001264"
     CDS             9852..10334
                     /locus_tag="IXF88_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1508003.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="IXF88_001265"
     CDS             10350..11111
                     /locus_tag="IXF88_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1508004.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /locus_tag="IXF88_001266"
     CDS             11242..12576
                     /locus_tag="IXF88_001266"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1508005.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="IXF88_001267"
     CDS             12883..13368
                     /locus_tag="IXF88_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1508006.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="IXF88_001268"
     CDS             13361..14044
                     /locus_tag="IXF88_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1508007.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="IXF88_001269"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="IXF88_001269"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1508008.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="IXF88_001270"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="IXF88_001270"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1508009.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17660..18289
                     /locus_tag="IXF88_001271"
     CDS             17660..18289
                     /locus_tag="IXF88_001271"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1508010.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="IXF88_001272"
     CDS             18341..18673
                     /locus_tag="IXF88_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1508011.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="IXF88_001273"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="IXF88_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1508012.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="IXF88_001274"
     CDS             19499..20872
                     /locus_tag="IXF88_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1508013.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="IXF88_001275"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="IXF88_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1508014.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="IXF88_001276"
     CDS             21927..22367
                     /locus_tag="IXF88_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1508015.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         7612 a         3873 c         4896 g         6066 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggcagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa tattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcacacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaaa
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaattt
//
LOCUS       DACESX010000041        21072 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.041, whole genome shotgun sequence.
ACCESSION   DACESX010000041 DACESX010000000
VERSION     DACESX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.041"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            156..1862
                     /locus_tag="IXF88_001277"
     CDS             156..1862
                     /locus_tag="IXF88_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1508016.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IXF88_001278"
     CDS             complement(2020..2922)
                     /locus_tag="IXF88_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1508017.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IXF88_001279"
     CDS             complement(2919..3623)
                     /locus_tag="IXF88_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1508018.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IXF88_001280"
     CDS             complement(3654..4295)
                     /locus_tag="IXF88_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1508019.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="IXF88_001281"
     CDS             complement(4333..4647)
                     /locus_tag="IXF88_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508020.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /locus_tag="IXF88_001282"
     CDS             complement(4635..5264)
                     /locus_tag="IXF88_001282"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1508021.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IXF88_001283"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IXF88_001283"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1508022.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="IXF88_001284"
     CDS             complement(5984..6916)
                     /locus_tag="IXF88_001284"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1508023.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="IXF88_001285"
     CDS             complement(6913..7689)
                     /locus_tag="IXF88_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1508024.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IXF88_001286"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IXF88_001286"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1508025.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IXF88_001287"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IXF88_001287"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1508026.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="IXF88_001288"
     CDS             complement(12014..13294)
                     /locus_tag="IXF88_001288"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1508027.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="IXF88_001289"
     CDS             complement(13313..14239)
                     /locus_tag="IXF88_001289"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1508028.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="IXF88_001290"
     CDS             complement(14252..15535)
                     /locus_tag="IXF88_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1508029.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IXF88_001291"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IXF88_001291"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1508030.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="IXF88_001292"
     CDS             complement(16323..17234)
                     /locus_tag="IXF88_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1508031.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="IXF88_001293"
     CDS             complement(17231..17695)
                     /locus_tag="IXF88_001293"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1508032.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="IXF88_001294"
     CDS             complement(17700..18398)
                     /locus_tag="IXF88_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1508033.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="IXF88_001295"
     CDS             complement(18385..18771)
                     /locus_tag="IXF88_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508034.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="IXF88_001296"
     CDS             complement(18918..20588)
                     /locus_tag="IXF88_001296"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1508035.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="IXF88_001297"
     CDS             20771..21004
                     /locus_tag="IXF88_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1508036.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
BASE COUNT         5896 a         4744 c         3738 g         6694 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aa
//
LOCUS       DACESX010000042        21052 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.042, whole genome shotgun sequence.
ACCESSION   DACESX010000042 DACESX010000000
VERSION     DACESX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.042"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            74..2671
                     /gene="adhE"
                     /locus_tag="IXF88_001298"
                     /gene_synonym="adhC"
     CDS             74..2671
                     /gene="adhE"
                     /locus_tag="IXF88_001298"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1508037.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2900..4006
                     /locus_tag="IXF88_001299"
     CDS             2900..4006
                     /locus_tag="IXF88_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1508038.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4023..5051
                     /locus_tag="IXF88_001300"
     CDS             4023..5051
                     /locus_tag="IXF88_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ1508039.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5057..5536
                     /locus_tag="IXF88_001301"
     CDS             5057..5536
                     /locus_tag="IXF88_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1508040.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5552..6847
                     /locus_tag="IXF88_001302"
     CDS             5552..6847
                     /locus_tag="IXF88_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ1508041.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6858..7682
                     /locus_tag="IXF88_001303"
     CDS             6858..7682
                     /locus_tag="IXF88_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1508042.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7847..8746
                     /locus_tag="IXF88_001304"
     CDS             7847..8746
                     /locus_tag="IXF88_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1508043.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8872..9741)
                     /locus_tag="IXF88_001305"
     CDS             complement(8872..9741)
                     /locus_tag="IXF88_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1508044.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9894..11354
                     /gene="rhaB"
                     /locus_tag="IXF88_001306"
     CDS             9894..11354
                     /gene="rhaB"
                     /locus_tag="IXF88_001306"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ1508045.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11351..12607
                     /gene="rhaA"
                     /locus_tag="IXF88_001307"
     CDS             11351..12607
                     /gene="rhaA"
                     /locus_tag="IXF88_001307"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ1508046.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12621..13466
                     /gene="rhaD"
                     /locus_tag="IXF88_001308"
     CDS             12621..13466
                     /gene="rhaD"
                     /locus_tag="IXF88_001308"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ1508047.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13468..13779
                     /gene="rhaM"
                     /locus_tag="IXF88_001309"
     CDS             13468..13779
                     /gene="rhaM"
                     /locus_tag="IXF88_001309"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ1508048.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13861..15012
                     /gene="fucO"
                     /locus_tag="IXF88_001310"
     CDS             13861..15012
                     /gene="fucO"
                     /locus_tag="IXF88_001310"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ1508049.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15139..15897
                     /locus_tag="IXF88_001311"
     CDS             15139..15897
                     /locus_tag="IXF88_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ1508050.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            15997..16641
                     /locus_tag="IXF88_001312"
     CDS             15997..16641
                     /locus_tag="IXF88_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1508051.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16648..17655
                     /locus_tag="IXF88_001313"
     CDS             16648..17655
                     /locus_tag="IXF88_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ1508052.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17671..18513
                     /gene="kduI"
                     /locus_tag="IXF88_001314"
     CDS             17671..18513
                     /gene="kduI"
                     /locus_tag="IXF88_001314"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ1508053.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18533..19336
                     /locus_tag="IXF88_001315"
     CDS             18533..19336
                     /locus_tag="IXF88_001315"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1508054.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19601..20941)
                     /locus_tag="IXF88_001316"
     CDS             complement(19601..20941)
                     /locus_tag="IXF88_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1508055.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         6770 a         3684 c         4744 g         5854 t
ORIGIN      
        1 tagaaaaata aatattgtat gatatacatg tgaattattt aacaaataac catgacccat
       61 taggaggata atgatggcta aagtaataga aaaagaaaaa acgaagacaa ttgacgtaca
      121 agcaatgatt gatgatttgg cagaaaaagc aaatgcagca ttgaaagaaa tggaaagctt
      181 tgatcaagaa aaagtagacc atatcgtgca tgaaatggca atggcagctt tggatcaaca
      241 tatgccatta gcgaagatgg ctgtagagga aacaggccgc ggtgtttacg aagataaagc
      301 aatcaaaaat atgtatgcgt ccgaatatat ttggcataat atcaaacatg ataaaacagt
      361 tggtgtgatc aatgaagatg tccaaaaagg gttgattgaa attgctgaac cagtcggtgt
      421 ggtttgcgga gtaactccaa caacgaatcc gacttccaca acgattttca aatcaatgat
      481 cgcgttgaaa acaagaaatc caatcgtttt tgctttccat ccaagtgccc aaaaatcttc
      541 tgcagaagca gcacgagtgg tacgtgatgc tgctatcgct gccggagctc ctgaaaattg
      601 tatccaatgg atcgaacatc cttcgatcga agcgacttct atgctaatga accatccagg
      661 tatcgcgatc gtcttagcga caggtggagc tggaatggtc aagtctgctt actctacagg
      721 aaaaccagct ttaggtgtag gtccaggtaa cgtaccagct tatattgaaa aaacagcaaa
      781 aattaaacgt gcagtgaacg atttgatcgt ttcgaaaaca tttgataatg gaatgatttg
      841 tgcttctgaa caagctgtga tcgtggataa agaaatctat gcagcagtaa aagcagaatt
      901 ccaagcacac caagtttata tcgtaaaacc agatgaattg caaaaattag aagatgcagt
      961 gatgaacgaa ggaaaatatg cggtcaatcc atcaatcgtt ggacattctg cgatggaaat
     1021 cgcaaaattg gcaggaatca gtgtaccaaa aggaacgaaa atgctgattg ctgaattgga
     1081 aggcgtaggc ccagattatc cgctatcaag agaaaaactt tctccagttc ttgccatgat
     1141 caaagctaac aacacggacc atgcgtttga tttgtgtgaa ggtatgttga atcttggagg
     1201 attaggacat acagcagtta tccattcaga aaacgaagag ttgcatgtca aattcggtct
     1261 tcgaatgaaa gcttgtcgta ttttagtgaa tacaccatct gcagaaggcg gcatcggtga
     1321 tatctataac gaaatgattc cgtcattgac tttgggatgc ggttcttatg gtaagaactc
     1381 tgtttctcgt aacgtctctg ctgtaaactt aatcaatgtc aaaactgtag cgaaacggag
     1441 aaataacatg caatggttca aattaccgcc aaaaatcttc tttgaaaaga actctctatt
     1501 gtacttagag aaaatggaaa acgttgaacg tgtcatgatc gtttgtgacc ctggtatggt
     1561 ccaattcggt tactgcgata cagttcgtga agtattggca cgacgtaaaa atgatgtgaa
     1621 gatcgaagtc ttctcagatg ttgaaccgaa cccatcgaca aataccgtat acgcaggaac
     1681 gaaactgatg gcagacttca agccagatac agtaatcgct cttggaggcg gttctgcaat
     1741 ggacgcagct aagggaatgt ggatgttcta tgaacaccca gacacatctt tctttggtgc
     1801 aaaacaaaaa ttcttggata tccgtaaacg gacatataag atcgacaaac cagaaaaaac
     1861 acaattcgta tgtatcccga caacatcagg gactggttct gaagtaacgc catttgcggt
     1921 catcactgac agtgaaacac atgtgaaata tccgttagca gattatgcat tgacgccaga
     1981 tgtcgcaatc gtcgatcctc aattcgtcat gtcagttcca gcatctgtca cagcagatac
     2041 cgggatggat gtgttaacac atgcaatcga atcctatgtt tcagtcatgg cttcagatta
     2101 tacacgtgga ttgagcctac aagcaatcaa gttagtcttt gactatttag aaaaatcagt
     2161 aaaaacacct gatatggaat cacgtgaaaa aatgcacaac gcttctacaa tggctggtat
     2221 ggcgtttgct aacgcattcc taggtatttg tcactcagtc gcacataaaa tcggcgggga
     2281 atacgggatc cctcatggac ggacaaatgc gattctttta ccgcatatta tccgctataa
     2341 tgcaaaagat ccacaaaaac atgcgatgtt ccctaaatat gactatttcc gtgcagatac
     2401 agattacgca gatatcgcaa aattcttagg tctgaaaggc gaaacaacag aggaattagt
     2461 cgaagcttta gcaacggctg tttatgaatt aggaaaatct gtcggcatca acatgagtct
     2521 aaaagctcaa ggagtcacac aagaaacact agatacaacc gtagaccgta tggctgaatt
     2581 agcttacgaa gaccaatgta cgacagctaa tccgaaagaa ccattgatct ctgaattgaa
     2641 acagatcatc attgatgctt acaacggata agcaaaagaa agcgtagaaa aaagataaga
     2701 aaatagatga aaaagaactt ggaaaacgtt gtttctccaa gttctttttt taatttccaa
     2761 gtaactgaaa gttacttttg ttatttaaca tttaggtcaa ttttagacag tttttgttca
     2821 aaaagcgata tttatagtgg acggtattac tgctatatta aggatataaa atgaaagcgt
     2881 ttaaaaaggt ggcgtaaaga tgattattaa agaagagatt tttgtaccta aagaattagt
     2941 gaaagaaaaa attgacctgc tcgttgaaaa cctgacaacg atcaaagacg ataatggtga
     3001 atttttgttg gactttgatg gactgaaagt agatgacaaa agctggacag tgtggaattg
     3061 gccacaaggt gtcggtctat atggtattta taaaaattac cagatgacaa aaaatcctcg
     3121 cgcttatcaa gtggtcaatg aatggtttga ggatcgtatg gaagaaggcg ctccgcccaa
     3181 aaacgtcaat acaatggcac cgctattgac tatggcttac ttgtatgaag acacgaaaga
     3241 ttcacgctat ttgccttatt tagaacaatg ggcagaatgg gtcatgaatg acatgcctcg
     3301 gacaaatgaa gacgggttac agcatgccac atatggaccg gaaaataaga atcagttgtg
     3361 ggacgataca ttgatgatga cagtgcttcc tttggcaaaa atcgggaaac tgttgaatcg
     3421 cccagaatat ttagaagaag ctcgttacca gtttatgatc catatcaaat acttgatgga
     3481 taaacgaaca ggattgtggt atcacggctg gacgtttgaa ggaaatcata actatgccga
     3541 agcattttgg gctcgtggaa actgctggtt gacgattgct attccagaga tcattgaaat
     3601 cttagaatta tcgaaagaag atgcattgcg caaattattg atcgaaacac ttgaagcaca
     3661 agtgcgtgca ttaaaaactt atcagtctga aagcggttta tggcatacgt tgttggatga
     3721 tccttcttct tacgtagaat cttctgcaac tgcaggattt gcttacggta ttttgaaagc
     3781 ggtccataaa cgttatttac cacaagaata taaagaagtc gcttatcgtg ctatccaagg
     3841 attgcttgaa caaatcgatg aaaaaggaga agttcaaaac gtttcaatcg ggacaggaat
     3901 gggggacagt ttagattttt atcggaatat cggaatcaca gcaatgcctt atggacagtc
     3961 gttaacggtg ctttgtttga cagaattgct cgtctcttat tgttaaaaga aaggaaaccc
     4021 atatgaaaaa aacatcactg ctgtttgcag caagttcgct tagcctaggt ctgctaggtc
     4081 taagcggctg cggaaataat tctagtgtgg caactgtcgc agatgcagat gtaaatgaga
     4141 tagttacctt gcgctttgct tatgcaagca acagccagcc agtaattgat gcaatgaatg
     4201 aatttgggcg tttagtaaaa gaaaaaacag atggttccgt acagatccag tatttcccag
     4261 atggacaact gggtggagaa cgtgaactga tcgaattgac acagtcagga gcagtagatt
     4321 tcacaaaagt aagtgcctca gcgttggaaa gtttctctaa agattattcg attttttcag
     4381 ttccttatct attcaatgat gaagaacatt tctttaaggt gatggataat gaagaaataa
     4441 tggagccaat ctaccaatcg accaaaaatt taggatttac cggattgacc tattatgatt
     4501 ctggtcagcg cagcttttat atggtagacg gtccgatcca tacgccggaa gatttggagg
     4561 gaaagaagat ccgtgtgatg caaagcgaaa ccgctatcaa aatggtagag cttttaggcg
     4621 gttcgcccgt acctatggga agtgatgaag tgtacacgtc tcttcaatcc aatttgatca
     4681 acggatcaga aaacaatgaa tttgtcttgt atacagctgg acatggtggt gtggctaaat
     4741 actattccta tgatgagcat acacgtgtgc cagatatcat catcatgaat gatgccatca
     4801 aagaacagct aactgatgag caggaaaaag ccattgaaga agcagcgaaa gaatcaacgg
     4861 tttttgaaat tgaagctttt gcaaaagcaa ttgaagaaga aaaagcttta gcacaagaag
     4921 aacatggagt ccaattcaat gatgtagata atacaccgtt tcgagaggca gttgccccac
     4981 ttcatgatat attcaaaaac gatcctacct acaaagacct ttatcaaaag atccaagagc
     5041 aaggagcgta gctgtgatga aaatgatttt aaacaaggta ttagagatcc tgggagctgt
     5101 tttgcttgtt gggatggtcg ctgttgtttt atggcaagta ttttccagaa cggttttagg
     5161 aaatccaaat acggtcacgg aagagcttgt tcgattcggt cttgtctggt ttgccatgtt
     5221 gtcttccgcg tatgttgtgg gacaaaaagc acatttagcc gtaacggttt taagtgaaaa
     5281 attaactggc aaaaaaagta aaattttaga agttgttgtt caaagtttat ttctggtatt
     5341 tgctgcgatg atcatggtgt atggcggatg gaatgctgtc acattgacaa tggggcagat
     5401 ctcgccttct ttaagtattc ctatgggata tgtttatctg tcagtacctg tatcgggggt
     5461 actcatcatt atctatagcc tgatcaatct aatcgataca ttaagcaaca agccagcaat
     5521 tcaagaaaaa atataaaaat ggaggaataa aatggcaata gaagccggat tagtgttatt
     5581 ttttgtattt gttgtgttgc ttgtattagg gatgccgatt gctataagta ttgcattctc
     5641 atcgatgatg actttattgt tagttatacc gtttgatgtt tctgctttta gttcagctca
     5701 aaaaatggtg ggaagtatca atagtttttc gctgatcgct attccgtttt tcgttctctc
     5761 gggcattatc atgaataatg gagggatagc taagaaacta gtggattttg cgatgttgtt
     5821 cgtcgggcgc gtgccggggg ctttggctca tacgaatgtg ttagggaatg ctttatttgg
     5881 ttccatctct agctcggcca ttgccgcttc taccgcgatt ggcggagtgt tgatccctca
     5941 gcaagtggaa gaaggatatg accggaagtt tgcgacagcg gtcaatatcg cgtcagctcc
     6001 gacaggtatg gtcattcctc caagtactgc ttttatcatg tattcacttg ttgctggtgg
     6061 tgcttcgatc tctgctttgt ttatgggagg ttatctcgta ggcgctttgt gggcgctagg
     6121 aatcatgcta gtagcattca tcgttgctaa aaagaataaa taccctgttg tcgaaaaagg
     6181 acagatggct catgctggaa aaataataaa agaggcgatt cctagccttc tattgattgt
     6241 catcattatc ggtggtatct tgacggggat atttacagca gtagaagctt cagcgatagc
     6301 tgtcatttac tccctgttga ttgctatgtt ttactataaa acggtaaaac taaaagatat
     6361 acctaatatg ttgtttcaat cagtagcaat gtcagggacg atcatgttct tattggctac
     6421 ttcttccatg atgtctttcg ccatggcgtt taccggtatt cctcaggcaa ttagttcgtt
     6481 gattatgggc gtaacggata ataaatttat tattctgtta ttagtcaatc ttgtgttatt
     6541 actggtgggt atgtttatgg atgtggcgcc tgctatcttg atttttacac caatattttt
     6601 accaatcgtc acaagtgtag gggttgatcc tgtacattac gggttgtttt caatcatgaa
     6661 tctgtgtgta ggctccatca cgccgccagt aggtacagga ctatatgtcg gtgcaagtgt
     6721 aggaggcgta aaagcagagg caatgctgaa accgcttctt cctttttatg gggttatctt
     6781 agtcgtcttg tttttgatta cttacttccc gcaaatcgtc atgtggctac ccaatatgct
     6841 gagttaggag aatagttatg ttgaacatta caattagagg acatgatctt tccgaagtcc
     6901 aaacgataga agatcttgca gaaaaaacaa aagaacaagg gattcataca ctgcagctcg
     6961 ccttaggtct ttcgtttcct gaaacacctt ctggcgcaaa tgagatcaat tctgggatgg
     7021 gaaattatgt caaacgagtt ttagaaaagc aagaagtgac cgtaggaatt ttaagttgct
     7081 atatcaatat gattcatcca gatttaacga tacgacaaga gttgctgaca aagttcgagc
     7141 agtatcttcg ctatgcttct tcctttggtg cgtcgatggt tgcttcggaa actggatgtg
     7201 ttttgccgga aatccaatat acggaagaaa atttcacaga tgaagcattt gctgaagctg
     7261 tgtccgttat ccgtcgcttg gtaaaagctg gagaaaaata ccaaatgatg gtggggatag
     7321 aaccaggttt gaatcatccg gtgtattctt tagcaagggt agagcagctg atccaagcag
     7381 tagattcgga ctatctagga atcattttag atccgaccaa tttgatcaca agtagtgatt
     7441 atcaagaaca agtccaattg gtagaagaag cttttgaacg ttttggtgaa aagatatgtg
     7501 cggttcattt gaaagatttt cgtgtcgaac aggaaaaaat cgtgccggta aatttgggag
     7561 acggtgtgat cgagtacaca aaaatcaaag aaatcatcat gaagaatcgt ccgtacctgt
     7621 atgtggttct tgaagaaaca aaagatgatg ggattcgtta tggaagaagt cttcttgaat
     7681 aatgtaaaga agacgttggt taactaatat ttagaatgga gggggcgggg ctagaagatg
     7741 ccacgtctct tcttttttat aagagtatat aaaagcagct ctttttgatt acaaaaaacg
     7801 tggtataata aaattgtgca taagtgaaca aaaggaggtg cggcacatgc tccaaaagaa
     7861 aatcagcggt gaacaagtca atacgatggt ttctgatcag tatgaaatct atcatgtcaa
     7921 agatgtcgtc agccaaaata aaacgatcta tcatcatcat gatttctatg agatccatgc
     7981 tacattaaag ggagaagcga ctttttattt gaacggacga caatttacga tcaaagcagg
     8041 aagtgtgctg ctcatccatt ccaatgattt gcatcgtatc gtccgccaat cttcagatgt
     8101 attcgagcgt gtctatatct ttgtcacccc ttcttttcta gaaagtcgtt ccacgccatg
     8161 gtctaatcta tcggcttgtt ttcaaccaat gggagagaag aggagtcgtg tattgcaaat
     8221 agaacaggaa gtattgaagc aaaaattatc atttatcgac catcctcctg ataaaaaaat
     8281 ttacggtgaa gacataaaat atgaacaagc tctggtagaa tacttgattt ttctcaatca
     8341 agttgtacaa aaagaagaga gtgcgcaaga acagaatgct gtagttccta atgagcgtgt
     8401 ggaacaaatg ttacaatata tctcaaaaaa tttatctcat ccgctttctt tagaggaaat
     8461 ggaaaagcag ttttttgtca gcaaatacca tatcacacga gaatttaaaa agtacactgg
     8521 atttacgttt catcagtatg taatgaagaa aaagctgatc tatgccaagc acttgttgcg
     8581 agaataccgc agctctagtg ctgtatatag taaaagtggt ttttcttctt atccgcattt
     8641 tctgaaagca ttcaagagag agttcggcat aacgcctaaa gaatttttga aacgtgatac
     8701 acagaaacag ttggttcatt atgaacattt tgaagatgaa caatgagaat gatcactaaa
     8761 aaataaccgc agtagctgct gttgtttaca tagaagcaga aactgcggtt attttatttt
     8821 tatctatata tttcaaaata ttttgaaaaa atgacgtaca tattatatga atcagctgaa
     8881 tttacgaaag tctctcggcg atagtcctgt ttttgctttg aacaggcggc taaagtaagc
     8941 aaaattctca atccctattt cttcggaaat cattcgaacc gtaaattctg tataccgaag
     9001 aaggtcttta gcgtaagaaa gttttttgct gatcaaataa tcgattggtg gagaaccgat
     9061 aaatttggaa aaatctttgc tcagctgata tttattcaat aagaattgtt gttctaattg
     9121 atcaagtgta atcgtttctt tgaaatgttc gtctaagtat tgcttcagtt tgtaaacgta
     9181 ggcgggaata tcctcttctt cgaaatctag ctgatacttt tgcaaaatca gttcattcag
     9241 caactcgtgg atagctacag aggactggaa atcggtacgg gcattcgtgc cttgttgcat
     9301 gtggatcaat tctgtgatca aatggtggat cttgttttct gatggatccc ctgttgaatg
     9361 gaaggtgtta tggccgtctt ttgtgaactc ctgataaaat gattggacgt tctgaccatt
     9421 caaatggatc caatccattt cccatggttc ttcgctgatc gtttgataac gctgatattc
     9481 ttggcagtca ataaaaaata agtctccagc ttttagctgt tgctgattat cattatatgt
     9541 aagcgaacct ttgccagaca aagtaaactt tatcaagtaa gaaggtaaat gtgctcgttc
     9601 agtatagtaa gggattggtg ctttgaagta accgatttct tggatataaa aaaataaatt
     9661 cctgcttctt tgtgtaggcg tcaagatcac tctttttgag tcttccgacc atgagtggtt
     9721 ggctttttcc cagtaatcca tgtaaacact cccaacttgt caagataatg caattatata
     9781 ccaaaatagt gtgaagcaaa atataagatt ctgtattatg atagcattgt taataaatga
     9841 taacgcttta ttaataaagt gctaatcaac aataaagtaa aagaggtgga gaaatgaatc
     9901 caacatttgt tgccatcgat atcggtgcat caagcggacg actaatgaaa agtacgaggg
     9961 atgcaaaagg ttctttgatc ttggaagaag tacaccggtt caaaaacggc tttcggaaca
    10021 aagacggtca cgatcgctgg aagatcgatt atcttatcaa agagattttg attggatttg
    10081 aaaaattaaa gcagacaggg attgatcgcc tttttgcggg gatcgataca tgggcagtcg
    10141 attactgtct gattgatgaa caaggggatc ggataaatga tccgattgct tatcgggaca
    10201 agcgtaccaa tggagcagta gaacagtttt ctgaaatcta ttctctagat aagttgtatg
    10261 agcagacagg gattcagatc cagcctttca ataccttgtt ccaattgttt gctgaagaaa
    10321 aagaaaattt ggcaaaagca cataaattgt tactgatacc tgattattta gggtatgtct
    10381 ttactggtaa catggtgacg gagaaaacta atgcatctac tatgcagctg ctgaatgcag
    10441 ggacaaaaga atgggaaata gaacttcttg aaaaaatcgg tgtctcgaag gaattatttc
    10501 caccattgac agagccggga acgatactag gagatctgca aaaagaacaa ttttcaaaat
    10561 atgatttgcc agatgtgacc tttatcacag tagcaagtca tgatacggcg tcagctgtat
    10621 taggaacgcc aggtcaagga gaacgttggg gttatatcag cagtgggaca tggtcgcttt
    10681 tagggattga aacaaagatc gccaatgttt ctcgcaatgc tttttctgaa aattatacga
    10741 atgaatgggg tgcccataac acggtgcgtt ttttgaaaaa catcatgggg atgtggttga
    10801 tccaggaagt tgcccgaatg caagagtacg agtacagtta tgcggaattg gcagagcttg
    10861 ctgcaaaaga aaaaccgctg cagcaagtga tggaaatcaa tgaccctcga tttttgaatc
    10921 ccccaaatat gatagaagag attcagcaat actgttttga aacagagcag ccagtccctg
    10981 tctcatcggg agctatcgct cgttgtgtgt atgataatct tgctctttgt tacgcacaag
    11041 agctgatcaa agtagaacac ttaactggaa cgaaattgga aaagctgcac atcgtagggg
    11101 gcggatcgaa taatcgtcta ctgaatcaat tgacagcaga ttatgccgga atcattgtag
    11161 aagcaggacc tagtgaagcc acagcaatcg ggaatgttgc catgcaaatg ctcagtacag
    11221 gtcagttttc gaatatagaa caagcaagaa atgtaatcaa acagtcgttt ccatgtgaaa
    11281 ctttttaccc agatgaaaat aagcaaaaca cgatggaatg gtacgtgaat aaaatcaaag
    11341 gagtaatcgt atgaacacga ttgaaagaaa ttacgaacaa gcaaaagaaa aatatgcaac
    11401 tatcggtgta gatacagatg ctgtactaga aaagatacag gacatcaaaa tttctatgca
    11461 ttgctggcaa ggagatgatg tcaaaggttt cttgacaccg gatggcgaat tgaccggtgg
    11521 aatcatggcg acagggaatt ttccaggagc cgcacgaaca cctgaagagc ttagacaaga
    11581 tctggagaag gcttattcgt tgataccggg caaacacaaa ttgaaccttc atgctatcta
    11641 tttggataca gaagaatctg tcgatctaaa tgaaattgag ccgaaacatt ttgaaaaatg
    11701 ggtagagtgg gcaaaaaaag aagaaatcgg tcttgacttc aatccgactt tcttttcgca
    11761 tccaatgatg aaagacggct ttacattggc tcatccagat aaagaagttc gtgaatactg
    11821 gatcgaacac gggaaaagaa gccgcaaaat cgcggagtac tttgggaaag aaactgggca
    11881 aacaagtatc aataactttt gggtacctga tgggatgaaa gataatccga ttgatcgttt
    11941 taccccacgg gaacgtttaa tggaatcact cgatgaaatt tttacagaag agttagatga
    12001 acggtatgta caagatgcag tagaaagcaa actatttggt ttaggcgctg aagcttatac
    12061 agtcggttcc catgaatttt atatgggtta cgggttgact cgtgacaaac ttgtttgtct
    12121 agatgctgga cattttcatc caacagaagt gatttccaat aaattatctt cattagcttt
    12181 attcagcaaa ggaatcatgt tgcatgtttc ccgtccagtg cgttgggatt cagaccatgt
    12241 ggttttgatg gatgatgaat tgcaggaaat cgctaaagaa ttagtccgta acgaattgtt
    12301 ggaaaaaaca aatatcggtc tggatttctt tgatgcaacg atcaaccgta tcgctgcttg
    12361 ggtcatcggt acgagaaata cacaaaaagc actgctgaaa gctatgctgg aaccagttga
    12421 gcgattgaaa gaaatggagc tggcttttga ctttacttca agaatggctt atacggaaga
    12481 attgaaagat ttcccgtatg ccgatgtatg gaattatttc tgttacaaaa atcaagttcc
    12541 agttggtttg gattggctgg aagaagtcca ggaatatcaa aaagacgtgt tggaattaag
    12601 aaaataggag tgtggctata ttgaaaaaaa tagatatttt acaagcgcct tatgtgaaag
    12661 aaatgatcga aacgacgtcg aatctttatc gcttgggatg ggatgaaaga aatggcggga
    12721 atatttctta tttactaaaa gaagaagaga tcacaccgtt cttggatcct gaaaaagtga
    12781 tccgccgcat ctcgatgatt tttgatgcca gccgtttagc aggatgctat tttattgtaa
    12841 ctggttcagg aagatatttt aaaaatgtag cgagcgaacc tgccgagaat cttggaatcg
    12901 ttcgagttgc tgaagatggc aaaacactgg aacttttgtg ggggctggaa aacgaatgcc
    12961 ttcctacaag cgagttgcca agtcatttca tgagtcatat cgcccgttta gaagtagacc
    13021 cagataatcg gatcgttatg cattgccatg ctagtcatct attagcgatg agttttacgc
    13081 atgaattaga cgaacgttcc tttagtcgta cgctatggca aatgtgtacg gaatgcttag
    13141 ttgtttttcc agaaggaatt tccatcattc cgtggatggt tccgggaaca aatgagattg
    13201 gggaagcaac agctgaaaaa atgaaagaaa ctcgattggt tttatggcca caacatggaa
    13261 tctacgggtc aggaaaagac atggatgaag tctttggttt gatcgagaca gcagaaaagg
    13321 cagcagaagt ctatacgtac gtcaaagcgc aaggaccaat cttgcaaaca atcacggacg
    13381 aaaacctgtg gcatttagca gatgcgtttg gtgtcactcc aaaagcaggt tatttggagg
    13441 aagttcatac gaaagcaggt gtctgacatg gtgaaaaaag ctttttgcat gaaagtttac
    13501 ccagaaaaac aagaagaata caaaaaaaga catgatgaac tttggccaga aatgcgtaca
    13561 gcacttcgtg aacacggggt catcgactat tcgatctatc ttgatccgag tacaagtttg
    13621 ttatttggtt acttagtgat cgaagatgaa aagttatggg aaaaaatggc aaatacatct
    13681 atcaatcaaa aatggtggaa gtacatggaa gatatcatgg agacaaatcc agatgcctct
    13741 ccagtcagtt gggaattgat gccagtcttt gaactgtagc aacaagcaac tgggcaaccg
    13801 gttgcttggg gttctttcgt ttttattgtg aaaaataata caaattagga ggaatagaaa
    13861 atgagtaatc gaatgatttt aaatgaaaca tcgtattttg gaaaaggagc gattgagtcg
    13921 atcgtccctg aatttcagca gcgaggattc ggcaaagcag ctgtcattac tgacaaagga
    13981 ttgatcgagc acggtatagc aacaaaagta acagaacttc tagataaagc agcaatcgct
    14041 tatgctttat acgacgggat cgtacctaat ccaacgatcc aaaatgtaaa agatggagtg
    14101 gcttttgtta aagaggcaga ggcagactgt ttgatcgcaa tcggtggagg ttcgccaatc
    14161 gatacggcaa aagccattgg gattattttg acgaatccag agttttcaga cgtcattagt
    14221 ttagaaggtg ttgctgatac gaaaaatcca tgtttaccta tattagctat accaacgact
    14281 tctggaacag cagcagaagt aacgatcaac tacgtcatca cagatgaagt aaatcaccga
    14341 aaatttgttt gtgttgatcc tcatgatatc ccaatcgtcg cgttcatcga cagcgatatg
    14401 atgatgggaa tgccgaaaaa attggctgca tcaacaggta tggatgctat gacgcatgct
    14461 attgaagggt ttatcacaaa aggcgcttgg gaaatgactg atatgctgca tttaaaagca
    14521 atcgaaatca tcggccattc tttggaagtt tctgtagatg gcgatcaaaa tgggcgagaa
    14581 aaaatggcat taggacagta tattgcggga atgggcttct caaatgtcgg tttaggactg
    14641 gtacacggga tggctcaccc gttaagtgca tggtacaaca ttcctcatgg cgtagcgtgt
    14701 gcagcacttt tgccaactgt catgaaatat aacaaagaat atactggaga aaagtaccgt
    14761 gagatcgctc ttgttctagg gattaaagga gcagctgaaa tgtctttaga agatgtaaga
    14821 gaagcggctt gtggtgagat cgatcggttg agcaaagcgg ttggaatccc agaaaccatt
    14881 tctgaattag gtgtaaaaga agcagatatt ccagcaattg cagaagatgc tttgcgagat
    14941 gtatgcacac caggaaatcc aagagaaacg acagtagaag aaatcattgc tttatatcaa
    15001 agtttgatgt aaaaacgaag cggatcttca acattaaatt gcctgtaaag agaaatggta
    15061 gtttatatct ggatatctct tttaaattga tggtatttcc gctataattt ttcttataaa
    15121 gaaataggag aatattatat ggaaaataaa aagccttatg gaactgtatt gatcaaagcc
    15181 tctaaaatac tagactattt agcggaaaac caagatgtct ctttgcagac gatcgcaaag
    15241 ggtgtgggaa tgacctcttc tacagtcttg aaaatactag atactttgtt gatgatcggc
    15301 tacgtaaata aaaattcaga gaaaaactat cgtttaggtg caaagcttat tcgctacgcg
    15361 aataaaaaca ttgaacaaat cgatttggta gagttgactt taccgttttt agaacagctg
    15421 cagcaaaaga tcgatgagac tatccactta ggggtattgg acaacaatga gatcttgtac
    15481 gtgaacaaac tagatccaaa acatcaaacg atccgcatgt cttcaaaaat cggaatcacc
    15541 cgaccattat acaattctgc gatgggcaaa gccgtattag cagaattcag tgaagagcag
    15601 tacactgatt atcttgaaaa gcatccgttg atcccttaca cagaatatac gatcaccaat
    15661 gcattacggt tgaaaaagga aatccaaacg gtaaaagaaa cccacatcgc atttgacgat
    15721 gaagaaatcg aacgtgatat tttttgtata ggagcctcaa tcgtgaaaaa cgaacaaatc
    15781 attggtgcat ttagtgtaag tatgccgaaa tatcgtttaa cagatgagac aaaagaacaa
    15841 ataatcagtg ccctcaagca aacaaaaagc gaaatcgagc aaagaattca aaaataatga
    15901 aaaaagcgtt tgcaattttt cttttctgcc gtataatatg ggtatagatt tccaagttat
    15961 aaaataaaat ttctaaatag gaaataggtg gagaagatga aaaaaattga tattttatct
    16021 cgattgaaaa acgccggagt gattgcggtg gtacgcggaa aaagcaaaga agaagctttg
    16081 aatgcttgcc acgcaatcat caaaggtggt ctaactggta ttgagttgac ttttacagtc
    16141 cctcaagcag atcaagtaat caaagaactt ttgtcattct acaaagacca accagaaata
    16201 gtgattggcg caggcacagt attagacgct gtcacagctc gcttggctat cttagctggc
    16261 gcagagtata ttgttagccc ttcctttgat caagaaacgg cagagatgtg caatctgtat
    16321 caaattcctt acttaccagg atgtatgact attaccgaga tcaaaacagc gctgaaaagc
    16381 ggggtagata tcgtcaaact atttcctgga agcgcttatg gaccaagtat catttctgcc
    16441 ttcaaggcgc cgatgcctca agtcaatatc atgccaactg gcggtgtgag tctggacaat
    16501 atgaaagagt ggttcgatgc tggtgtcgta acagtcggag taggtgggaa ccttttagca
    16561 cccgcagcta caggagattt tgacaaagta acggaagtcg cgcaacagta tgcagcaaaa
    16621 atgaaagaaa tcaagaggta gctgcttatg ggaaaagtag tgacactcgg agaaatcatg
    16681 ttgcgattgt ccacagagca gggaacccgt atcgattcaa gtgaacgatt cgctgcccat
    16741 tatggcggag gggaagcaaa cgtagcaatt tctttagcta attacggaca tgaagcgatt
    16801 tttgctacaa aagtacctga taacgcattg ggaaaagcag tcaaaaagca cttgcaatgt
    16861 tatggagtag atacgaaaca ggtattatct ggtggatcaa gactaggtac ttattatatg
    16921 gaaactggta gtggcgaacg cgccgcctca gtgatctatg atcgtgccgg atcaagtttt
    16981 gctgaaatgg atcatttgga atggtcgcta gaggagttat tccaaggaat agatatattt
    17041 catgtttcag gaatcacccc agctttgtct aaaaaatggc aaaaaatgac gaaacaggtg
    17101 atagaagctg caaaaaaagc tggctgcctg atcagcttcg atatcaacta tcgtggaaaa
    17161 ttatggacac agcaagaagc cagcgaagta atccatcagc ttttaccttt agtagatatc
    17221 tgctcagctg gaaaaatgga tgctctttat ttgttaggta tcgagaaagc gccagaagca
    17281 gaagaacaac ctctaattta ttattatcaa aaaatgcagg aaaagttccc gaatattcag
    17341 gtgttttatt cgaccaagcg taccgttcat tcagcaagtg aaaatgaatt gaaggggaca
    17401 ttgtggatga accatgaata tgtggaatcc caagtccata gaatcacccc aatcgtggat
    17461 cgagtaggtg gcggagatgc ttttgcaggt ggcgtgctcc atgggatttt atccaatatg
    17521 tctccgcaag aaatcattga ttttgctacg gcagcttctg ccttgaagca tacgatccat
    17581 ggggactgca accaatttag tcaagaagaa gtcgcacaat ttttagcatg cggctctgga
    17641 aaaatcattc gatagaagga gtagaagaaa atgcaagaaa tggaaacacg ttatacacac
    17701 agtccagaag atatccgtca ttattcaacg gaacaattga gaaaagaatt tttagtagaa
    17761 aaggtatttg aaccagggaa aatcagtttg acttatacgc acaacgatcg aatgatcttt
    17821 ggtggagtga cacctactga aaaagaatta gaaatcacat tagacaaaga actaggtgtt
    17881 gattacttct tagagcgtcg tgaactaggt gtgatcaata tcggtggacc aggattcatc
    17941 gagatcgatg ggcaaaaaga agaaatgaaa aaacaagacg gatactatat cggaaaagaa
    18001 acacgacatg ttgtattttc ttctcaagat gctgaaaacc cagctaaatt ctatattagt
    18061 tctgttccag ctcatcataa atatccaaat gtaaaaatca gtatcgacca agtaaaacca
    18121 atggagactg gtgaaggcct gacactgaat gaacgtaaaa tctaccaata catccatcca
    18181 aatgtatgcg aaagctgtca attacaaatg ggttatacga ttttagaacc aggaagcgct
    18241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctactttgat
    18301 tatgccaatg aagatacacg tgtgttccac atgatgggca aaccagatga aacaaaacat
    18361 ttagttgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
    18421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
    18481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
    18541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
    18601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
    18661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
    18721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
    18781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
    18841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
    18901 cccattcatc gtttcaaaag cagttattcc ggatatgatc gaaaaaggcg gcggtaaaat
    18961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
    19021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
    19081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
    19141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
    19201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
    19261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
    19321 cggaaaacaa ccttaaaaga gctttagtac actgtttagt ggagaggttg tgaaatcagc
    19381 ggattgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
    19441 ggacagaagc atttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
    19501 tttgtgaatt tcagcttatt tccgaaggag ttgcttttag gctcttcgtc aatgagtgtt
    19561 ggtgaagaaa ttcaatagaa taatcgagat actcacacgg ctagacagct atcttgtcta
    19621 gccgtgttgt tgttctggct ttagtcagtt gggtagtgtt cttagttttc ttgaaatatt
    19681 ttcctcgata taaaaagatt cgttctcggt agttttgaaa gtttctaaag ccataggcaa
    19741 ttcgttttag tactttgata tggttgttca aacattctat agggccgttg gaatagggaa
    19801 ctcgcaacgc tcgtttgatt tctgttcgat atttcttgaa ggtcgtgaac actggttgat
    19861 agtgctctgg caactccttg tagtcttctt ttaggagcgc gacgaattca ggaacatctt
    19921 gtcttctgat agcggaaaga aaaacctggt agacttcgta gcctcgcttt aaggagtcat
    19981 cataatataa tagccgatcg actatctccg cttctgtcag atgatctcga aatgacggac
    20041 gccagtagcg atgctgacca ttcaattccc acgcattctt ttgaagtagt ctccagtatt
    20101 tctttaagtg cttcgaccga cgatcccctt ttttaaaggt cttcattact tgaattcggt
    20161 ggttggtaaa ggcccgaccg atgtgttggc taatatgaaa gcgatcggcg acgattcggg
    20221 cattcggaaa acactctttc accaatgtgc gatagggtgt atagaaatct gaaaccacta
    20281 gacgtacttg ttcacggact tttctagggt agcgcaagaa atagtcgcgt aaaggattga
    20341 gttgccgatt ttccacaata tcaatcagtt gatgggtctt gccgtccatc atgacaaagc
    20401 tcatggctcc cgagacgttt ttcaccgatt tgaattcatc gaaacacacc acttctggta
    20461 acggctggtt caaagaaacg gttttaggcc gaaggctgcg aagtattcga atgacagtgg
    20521 tcggagaaat gtgcttcata cgtgcaatca aagacataga aataggctca cttaatagct
    20581 caagaatggc ttgtttgact ctgcgggcaa tggagcagcg acgatcgcta actgaatctt
    20641 ctgctaaaaa agtctttcca cagtgtttac atttgaagcg ctgtttgtta atccgcacaa
    20701 tggtcttgta gttactgaca tcattcaata agatggatac ttttttccag ccccatttaa
    20761 tgatcgtttg cttattttga tagtggcaat ggaagcattc ctctggttga taggtcaatg
    20821 tgccatcaaa aacaaaacat gtttcgtcat taatgcgctc tttgcgaaag gcatcttcat
    20881 gaaaggtaag atttaggtct agaatatcta gtaactcttt ggtaaactct gaataggaca
    20941 tcttgaaaca ctcctttgaa tggttttcgt cgactttatt ctaaaggatt tcaaggtgtc
    21001 tgtttgcgtt tattagaaaa aagaaaaagt attggcaaca gaaggcgtca cc
//
LOCUS       DACESX010000043        20087 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.043, whole genome shotgun sequence.
ACCESSION   DACESX010000043 DACESX010000000
VERSION     DACESX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.043"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            299..1960
                     /locus_tag="IXF88_001317"
     CDS             299..1960
                     /locus_tag="IXF88_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1508056.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IXF88_001318"
     CDS             complement(2030..2377)
                     /locus_tag="IXF88_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1508057.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IXF88_001319"
     CDS             2461..3402
                     /locus_tag="IXF88_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508058.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IXF88_001320"
     CDS             3403..4446
                     /locus_tag="IXF88_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508059.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IXF88_001321"
     CDS             4452..5522
                     /locus_tag="IXF88_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508060.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IXF88_001322"
     CDS             5512..6465
                     /locus_tag="IXF88_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1508061.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRYDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IXF88_001323"
     CDS             6477..7487
                     /locus_tag="IXF88_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1508062.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IXF88_001324"
     CDS             complement(7772..8593)
                     /locus_tag="IXF88_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1508063.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IXF88_001325"
     CDS             complement(8690..8968)
                     /locus_tag="IXF88_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508064.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IXF88_001326"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IXF88_001326"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1508065.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IXF88_001327"
     CDS             complement(11724..12311)
                     /locus_tag="IXF88_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508066.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IXF88_001328"
     CDS             12486..13808
                     /locus_tag="IXF88_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1508067.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IXF88_001329"
     CDS             13963..14739
                     /locus_tag="IXF88_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1508068.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IXF88_001330"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IXF88_001330"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1508069.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IXF88_001331"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IXF88_001331"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1508070.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IXF88_001332"
     CDS             16629..17258
                     /locus_tag="IXF88_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508071.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IXF88_001333"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IXF88_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1508072.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IXF88_001334"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IXF88_001334"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1508073.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IXF88_001335"
     CDS             19445..19981
                     /locus_tag="IXF88_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1508074.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         6544 a         3600 c         4097 g         5846 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggtatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacggct ctttgtcaaa tgatgttggt aaagagaaat cttagaataa tttggacaga
    20041 acagaaagaa gaaaacctct ttctgttctg tttatttggt taaaaat
//
LOCUS       DACESX010000044        19901 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.044, whole genome shotgun sequence.
ACCESSION   DACESX010000044 DACESX010000000
VERSION     DACESX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.044"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            64..252
                     /locus_tag="IXF88_001336"
     CDS             64..252
                     /locus_tag="IXF88_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508075.1"
                     /translation="MDIYKKLASKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPAD
                     FYRKMQIINYQPLAETHA"
     gene            complement(896..1849)
                     /locus_tag="IXF88_001337"
     CDS             complement(896..1849)
                     /locus_tag="IXF88_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1508076.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1872..2627)
                     /locus_tag="IXF88_001338"
     CDS             complement(1872..2627)
                     /locus_tag="IXF88_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1508077.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2624..3583)
                     /locus_tag="IXF88_001339"
     CDS             complement(2624..3583)
                     /locus_tag="IXF88_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1508078.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3576..4526)
                     /locus_tag="IXF88_001340"
     CDS             complement(3576..4526)
                     /locus_tag="IXF88_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1508079.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4676..5128
                     /locus_tag="IXF88_001341"
     CDS             4676..5128
                     /locus_tag="IXF88_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1508080.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5217..7484)
                     /gene="gshAB"
                     /locus_tag="IXF88_001342"
     CDS             complement(5217..7484)
                     /gene="gshAB"
                     /locus_tag="IXF88_001342"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1508081.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7671..8669)
                     /gene="ftsY"
                     /locus_tag="IXF88_001343"
     CDS             complement(7671..8669)
                     /gene="ftsY"
                     /locus_tag="IXF88_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1508082.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8680..9489)
                     /locus_tag="IXF88_001344"
     CDS             complement(8680..9489)
                     /locus_tag="IXF88_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1508083.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9498..13079)
                     /gene="smc"
                     /locus_tag="IXF88_001345"
     CDS             complement(9498..13079)
                     /gene="smc"
                     /locus_tag="IXF88_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1508084.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELTKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13099..13785)
                     /locus_tag="IXF88_001346"
     CDS             complement(13099..13785)
                     /locus_tag="IXF88_001346"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1508085.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14133..15914)
                     /locus_tag="IXF88_001347"
     CDS             complement(14133..15914)
                     /locus_tag="IXF88_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1508086.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15940..16854)
                     /locus_tag="IXF88_001348"
     CDS             complement(15940..16854)
                     /locus_tag="IXF88_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508087.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16881..17843)
                     /locus_tag="IXF88_001349"
     CDS             complement(16881..17843)
                     /locus_tag="IXF88_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508088.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17843..18787)
                     /locus_tag="IXF88_001350"
     CDS             complement(17843..18787)
                     /locus_tag="IXF88_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508089.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18788..19792)
                     /locus_tag="IXF88_001351"
     CDS             complement(18788..19792)
                     /locus_tag="IXF88_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508090.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5521 a         4271 c         3541 g         6568 t
ORIGIN      
        1 aatatcgagg gttcttatat tgaagtaaaa acactctttg tcaatgaaga taaaaccaaa
       61 gcattggata tttataaaaa attagcttca aaaaaaacga atagttttgg attgatatta
      121 agcgagtata aaatcaaagc agaagagagt tatttctatc aattactaaa acggtggagt
      181 aagcttcccg ccgattttta tcgaaaaatg cagatcatca actatcaacc gcttgctgag
      241 acacatgcct aaatagaggt tgtgaaatca gtgctttgag ctaataccga aggtcagctg
      301 attgaacatt gtttagaaga gattgtgaca actgcgattt gctttgagta ataagaacgc
      361 tggacagaaa atagcttctc atattttcta gtctagtggg cttattgccg agaagcagca
      421 gatggaacat cgtttagaag agattgtgac aactgcgatt tgttttgagt aataagaacg
      481 ctggacagaa aatagcttct catattttct agtctagtgg acttattgcc gagaagcagc
      541 agatggaaca tcgtttaaag aagagatcgg tacaaaataa aaatatctaa atttaagttt
      601 tatctaagtt tcgctttgta cctttgattc aaatcaaagg aggcgatctt gatgctgaaa
      661 aaatggatca cactattttt tgccactccc cattctagaa tcaacccgta tgatgtagat
      721 aatgaattca cgttgaaaaa agatttctca aataaataaa aaccatccgt aaaatcatca
      781 atacgatttt acggatggtt tttgataact tctttgagga taggctcttg ctatttaaga
      841 atgtaagaaa cattattgtt acatagtatg agagtttaat cctctgttat ttatgctatt
      901 taaatgcttg attcacatct tcgatcatca gtttcattga ttctaaaccg cccccgctta
      961 aataccagac atctggttca agggagatga tctgctgatt tttaccagca ttcgtttgtg
     1021 ctaccagttc attggcagaa atatcatctt tgctgtcgtc tccaccaata gcttttgttc
     1081 tgtctactac aaataagata tccggatttt tttccagcac atattcataa gaaacacttt
     1141 gaccgtgagt ggatgcttcg atttgatcat ctgcttgttc aaagccaaat aaatcatgga
     1201 tgaaaccgaa tctagaacca gatccgtagg ctgataattg cccttcattc acaagtgtga
     1261 ctagtgcttt tttgtcagta gcttctgctt tctttttcgt ctgatcgatt gcactagata
     1321 attcctctaa ttgagtttta gcttcttctt gcttaccaaa gatctcccct aaagcagtga
     1381 cattttgctg gaaagattcc catggatttt tcgcatctaa agaaaggaaa atggtcggag
     1441 caatttgact tagatctttt tgaaaatctc tttgtcggcc agaaatgatg attagatctg
     1501 gctgaagctg attaattttt tctaagtctg gttcttttat tccacctgct gattctactt
     1561 ttttatagga tgaaagatac tcaggaaggc tgcttgtagc tgctccaact accttatctc
     1621 caacaccaag agcatccatc gtatctaaag aaccattgtc gaatacgacg actttttctg
     1681 gattatttgg cacctcgatt tggtctccat tactatctgt tatcgtcaat gtagtttcag
     1741 tttgtgtgct gctttttttt gtgtctgcct gactggtgtt gttgctattt ccacatgctg
     1801 ctaaaccaaa tgatgctaga atcaagatac ctactactaa ttttttcatc tcttctctcc
     1861 taatgttagt tttagaaata aagacagaat ctgcggcctt caatctcaca aatccgtatg
     1921 tccatttcgt ataattcatc caatacatct ttttttatca tttcttgggt cgttccttgc
     1981 gtaaataact gtccatcttt cattgcaaca atttcgtctg cataacttgc agcaaagttg
     2041 atatcatgaa gaacaataac aattgttttc ccgatttctt gtactaaatt ttttaaaatc
     2101 tgcatgattt gaaccgcaaa attcatatct agattgttca aaggttcatc cagtaaaata
     2161 taatccgtgt cttgcgctag caccattgca atatacgcac gttggagttg accgcctgaa
     2221 agagtgtcaa tcgattgatc cgctatttct tctaacccaa ggtgttccag tgattcctga
     2281 acgatttcct cgtcttttgt cgtcaatttt cctttcgtat aaggaaatcg tccaaattct
     2341 actaattctt tcacggtgat ttggacattt atttcattcg attgtcgtag gatcgataat
     2401 tttttggcta attcattgga tttccaagcc tttacttcgt tatggtctaa atagatttca
     2461 cctgtatctt ttggtatcaa acgactgatc atcgacaaca ccgttgactt tccagcacca
     2521 tttggaccga taaaagcagt caacttcttt tcaggaatcg ttaactgtac atctttaacc
     2581 actgattttc catggtacag tttattgacc ttttgtaaat caatcatagg atactcttcc
     2641 tttcgcgaat caattttgtg acaaaataaa ttcctccgac aaattcaacg acgacactca
     2701 ctgttgtatt ccagccgaaa atctgctcta ctgcgagctg tcctcctgtc agaaacagca
     2761 tagctaccag aaagcttccg ataaatagct gaccatgacg ataagtagaa aagacacgat
     2821 aactgatatt tgcgacaata aaccctaaaa aagtaactgg acccacaaga gccgttgatg
     2881 tccctgtcaa aagactgaca caacataaaa tcaaaaattc aaaacgatct gtgtctatcc
     2941 cgaggtttac tgcttgttca tttccgagat ggaaaacgtc tagcacatgt cgatatcgaa
     3001 ataacaggta ccagcagccg ataatgatca aagaagcagg caaaagatac tgactctgga
     3061 tattgccaaa gcttgcaaat aatctgcctt gaagcaaatc atattcattc ggatccatga
     3121 tgacttgtaa aaaggtactg acactaccaa aaagcgtacc taaaatcatc cctgacatca
     3181 gtaataaaaa aagattcgct ctcatttttt tcaaaagaaa gaaaaataac aatagactca
     3241 gcccgaccat cagcccaacg ttcaataaga aaaaaactgt acctgtctgc tgaagcatcg
     3301 tcactcctcc gataaagaag aaaaggaccg tttggacaag gacatataac gactcaagtc
     3361 ccaatactga aggagtcaaa aactggttat gcgtaattgt ctgaaagctg attgtggcac
     3421 tgctgacagc tagcgagaca aggataaatg caaaaatttt cttactcctc aatgcacatg
     3481 caaaatgcca gtttccatat gtatggatcg tcaagtacat tcctgtaaca aataaaacaa
     3541 gaacaagcat accaataaaa atagatggct tttttttaag catatcgtcg tccccttatc
     3601 agaagataaa ggaagatagc cccaccgatg attccaacga tcacactgac tggtatttca
     3661 taaggaaaaa tcaacacgcg agcgacaaga tcgcaaaata ctaagaacaa actacctgct
     3721 aaagcagtta cgccaattgt tttgtgcact tgatctccat atattctaga gacaagattc
     3781 ggaatgatca ctccgataaa aggaatgctt cctaccatga tcaacatgac tgcactagcc
     3841 aatgatacta agccaatgcc taaaaactgc atcttctggt aatctattcc cagatttact
     3901 gcaaacgatt cgcccagacc aatgatcgta aatcggtaag caaatagcca aaccaagagc
     3961 aacaatggaa caacagcata caataactca taatttcctc gtatcacagt cgcaaaattc
     4021 ccttgaagcc atgaagtcat attttgtacc aactggaatt gataagcaaa aaaagtagca
     4081 actgaaccaa tgatattccc taacatgact ccaatcaacg gaagcatcaa ttgatttttc
     4141 tggaggaaca atcgcgtaag ggataaaaaa agcaatgttc ctgcaaaagc aaagcaaaat
     4201 gctgtcaagg atcggacaat caaggatgca ccaggtagga aaatcatagc cactaaaata
     4261 cctaaacgcg cactatccat cgtgcccgca gtcgttggtg aaacaaattt attttgggtc
     4321 aaatgttgca tgatcaaccc acaaatacta ctggtcgctc ccacaaggat caaacaaatc
     4381 gttcgtggca aacgagtcac tagcaagatt tgtaagcttt gcccatctaa ctggagcagt
     4441 tctgacaatt gtacattcga tactccgatg aacaatgaaa tactacttaa aataactaat
     4501 aatgctacta gtcgtccgta gcgcatccaa tccacctttc taattgagaa tgattttcat
     4561 tcttatttta agtgaatcgt tgaacaaaag caaccgcttt ttcatatgtt ttaggtatta
     4621 ttgggaatat gctataatcg taagaaagac aaagagaaga aggagggtat tttttatggc
     4681 acaagatgat gtaaaaagcc atttggataa gggactttat ggtacacctt tagtgaaccc
     4741 tgaagaacag cataaatatt tagggacatt cagggaacgc tgctatctta gtatgactgt
     4801 tgctgaaatg atccaaccaa aaaacaaaga aaacttttta aaagaactca aaaaacatcc
     4861 agatgcttcc atactcttga atggggctct acccctttct gtccaaaacc aatatatcca
     4921 gctagcaacg aaaaacaata gccgtttcac tgtagtcaac gattacgtca ctgcaaatcc
     4981 tgaggaattc ggattgctct taacagcaaa agaagcagta aatgaacctg tgattgatat
     5041 cgaaaaaaaa tacccagaag aaaactcttc gccagctgaa aaagaaaaac caaaaaagaa
     5101 aaaaagcttc tgggatcgtt tattttaaaa cgaaaaaaca cataaatttt tacatgcaaa
     5161 aaaagctttt gatcagattg acatcaaata ggacaatctg tcaaaagctt tttcttttaa
     5221 aatacttcag gatataacaa tttcaataca ttcatcgtca atcttctgcc ttttcctgca
     5281 aaaggataaa catgcatatg catggctgga ttgaaatttg cttcgatgat cccatatgct
     5341 tttttatcag ttgtaggatc gatttcttta tctggtataa tcaaatcgat accactaatc
     5401 ttagctccta gtgcttctac cgcagaaacg gcgatttttt tgtaagcttc agaaaattca
     5461 tcagtcatat cgatcgaatc accacctgta ctgatatttg agttctccct tagataaaca
     5521 atttgattgg cttggggaac tgattcgatc gtcaaccctt gttctttcaa catcaattgt
     5581 tctaattcac ctaattggat caattctaaa ggtgcacgat gattcgttcc acgcaaaggg
     5641 tcactatttt tttcttcaac aagttctttc actgttcgga tactgtcacc aataacatta
     5701 gcagggactc gcaacatgat ggcttggact tctccgtcga tcacaaagaa acgatattcc
     5761 gttcccggca taaattcttc tactaaaacc gaactgtctt cacgaaaagc aatggctaaa
     5821 cctgcttgat aatcttccag actagctcct tccttgaaaa tcgtgatacc taacccgtaa
     5881 ttggttgatt taggtttgat cacaaatgcc tgctcggcaa atctcggata agcttttacc
     5941 gcttcttcca tggatgaaaa ttctgcgcct cctggtaccc ggaacccagc ttcttttaag
     6001 acttttttcg ttactgtttt gttttccatg ataagtggga cgatatagct gtccttactt
     6061 gtcatgtttg catttttcac atattctaca tggtcttgga attgcagacg caaaaattga
     6121 tccgactcat ctaatacttt gacttggaca cctttttgga tggcatcgaa cataaatatc
     6181 tgagtagaaa gttccatttc gcgaaaaccg gccaactgat agggacgttc atggctttct
     6241 ttataatatt gatgtcccaa tgccacagca acttcttgct gactcgatag ctgtgcttct
     6301 tgatagagtt tcgctgctaa cgtttgttca ggatgatcca tttgagtcaa tgcttcctct
     6361 actaaataaa gagaatccaa ccaatccaat tcaaccagca tttgcttcat tccttcaaga
     6421 atttctctac cttcttgatg aaattctgtt tgatctgatg gttgttctag tgctactttg
     6481 ttatttcgag tcgtaccttc tgctacccat tggtcacagt cttctttttc ttctgtccat
     6541 aacatgtaca tcaggaataa ttgtaagaac cgaacttgct ctggattgat acccaatctt
     6601 gcataaggat tcaaatcgat attccgcaat tcaatataac gaacacctgt tgttgctaaa
     6661 tctgcgactt tctttccgcc tcggaaacga agcggtgtat aaaattcttt ctcctccgaa
     6721 agtttccctt cttcgatcat tcgctcgata tctgctaaat actgtttcat agaagcatag
     6781 gaaactttca catttggatg attcgtatag cccagcgcac tgttgcgaat actgcgaaca
     6841 ggttcttgcg gatgtgattc atctaaaaag tagttcttct cactcattgg tgaagcaccg
     6901 aataaataag tgatcatcca gcgatagcgc ataaagtttc tcgctgtttt caaataaagt
     6961 tctgttttga attcggaaaa atcttgaaat tcttcctgat ggctgaacaa tgttcttagc
     7021 aactcatcgc caaattcaaa attgaaatga ataccgctta ccatctgttt tcgcttcccg
     7081 tattcttttg ctaaataacg tcgatacaag acatcttcga aattttttaa ttttgcaata
     7141 atgatctctt cgtctttttc cggtaaggca ggtggcatac ttaatggcca gatcatctct
     7201 tctttcggta tagaacgagc agtcacatca taaacagcag cgagatactg aaataattcg
     7261 ggaatagaat cggtaacagg ggtgatcatc tctgtttgtg tttcgctaaa atctgtttgg
     7321 atataggggt gataggatcg atcgccaaag attgctggat gatcggtttt tgctagattt
     7381 cctgcaagat caacccgctg tccttcccgt tctataccaa atcgagcttg gtcgataaaa
     7441 gggcgcgcat tgacatgcaa taataattgc ttaaaattca tcattaagtg acctccaatt
     7501 catatgaatc taattataat caaaaagtct aagaaaaaga acgaaattgt ttgagaaccg
     7561 cttacaaata agtataaaaa tgaaggcatc aaaaacacag ctatgttaaa aacaatcatt
     7621 caaacaagga gctgcaactt ttgtcacagc tcctgctttt tagacatggg ttattcttct
     7681 tttagtaatc ctttgaacaa tccaacgaca aaatcatttg gatcaaacgg ctccaagtca
     7741 tcgattcctt caccaagacc gaccaatttc actggtaaat gcaattcatt acggatagca
     7801 agtacgatac cgccctttgc tgtaccatct aattttgtca aaacaagacc ggtcacatct
     7861 gttgtttctt tgaattgttt tgcttgaacc atcgcgtttt gaccagtggt tgcatctaat
     7921 acaagaagtg tttcttgggg tgcttctggt aattcccgct ggattactcg ttttattttt
     7981 tctaattcgt tcatcaagtt cactttattt tgcagtcggc ctgcagtatc aactagtaaa
     8041 acatctgcct gctctgtctt tgctctttcc aaagcatcga atacgacagc tgctggatcg
     8101 cctcctgcat tgccacgaac aacttcaaca ccagcgcgtt cgccccagac aactaactgg
     8161 tcgattgctc cagcgcggaa ggtatctgct gctgccatca atactttttt accttcgttt
     8221 ttgaactgat gggcaagttt accaatactt gttgttttcc ctacaccatt aacaccgaca
     8281 aacaaaataa ctgttagtcc gtcttcttgg atatttagtt catttacttc attgattcct
     8341 tccgcttcat agagatccac aagtttctcg atgatcgcat tttggacttc tgccgatttt
     8401 ttcgcatttc tgagtttcac ttcttgacgt aatgcttcgg tgattcgtat cgctgtttca
     8461 aaacctacat ctgcaccgat aagtgtttct tctaattctt caaaaaagtc ttcatcgact
     8521 gttcgaaaat tggcaaacaa ctcattcatc cgttcaccaa atgttttacg cgtcttttct
     8581 aaacccttgt cgtacttctc ctgtacttca acttcttctg ttttctcttc gccagtaaaa
     8641 gcacgcttga ttttatcaaa aaatcccatt tgactccccc tactctaata caaatttttg
     8701 gatcactttg gctacgccat tttcttcatt gctggctgtc acgaaatctg ctgcttcttt
     8761 gacgaaaggc acagcattct ccatcgctac gcctagtcca gcatattgga tcatcggtaa
     8821 atcattttcc tcatcaccga tcgccatgat ttcttcttgt ttcaaaccaa gatcttttgc
     8881 caataaagaa ataccataag cttttgtaac accttttggc ataaattcca aaagcatact
     8941 tcttgttttg atgatctcat acatttcatg gaattctgct ggtatttctt tgattttagg
     9001 atctaattct tcttggtcat aagcgattac tgccttatta agcacaaatt catctgtaat
     9061 atcgcttata tccgctggtt ggaattgcag cagcttattc aacacattgt acaaggattg
     9121 cttccttggg gcactaggca gctgtaaaac cacattgtca gacaaaacat ctagtggcag
     9181 atccaattgc tgagccagtt tgtataagcg ttggatatct gatacttcca tcactttctt
     9241 ttccatgatt tcaccagtat catttttttg taccaaaccg ccgttgaaag tgatgctgta
     9301 gtcaccttca tctaccagtc ctaattcttg cagataatgc gccatcgcag ctaaggggcg
     9361 tcctgtacat agaacgatct tgaccccttg ctgttttgct tgtgctaatg cttgtttatt
     9421 ctcatctgaa atcagctttt gttcatttaa taatgtaccg tccaaatcga ttgctactaa
     9481 tttgatcatt ttcgttccta cactttcgtt tcgttgattt tcgcctcttc attgatatct
     9541 tccatccgta cagaaacgat ttttgataca ccggattcct gcatcgtcac tccgtacagt
     9601 acatctgctg cgaccatcgt tcctttgcgg tgtgtgacta cgataaattg tgtgtcatct
     9661 tggaaatcgc tcaagtaacg accaaaacgt ttcacgttgg cttcatctaa cgctgcttct
     9721 acctcatcta agatacaaaa cggaacagga cagacacgga taatggaaaa gagcaacgcg
     9781 atggctgtaa gtgctcgttc tcctccagaa agcaaactta gactttgcag ttttttgcca
     9841 ggaggttgta cttcaatctc aatacctgtt ttcagcaaat cagttggttc cgtcagtact
     9901 aattcagctt taccgccgcc aaacatattt ggaaagacaa ttttgaattc ttgacggata
     9961 gcttcaaata cttctttgaa tcttgtccgc acttcatcat ccatctcatc catcgtttca
    10021 aacaattggt ttttcgctgc taacagatca tctcgctgtg ttgctaagaa tgtatgtctt
    10081 tcactgactt gttcgtattg ttcgattgaa tttaagttta ctggtcccag tttttcaatc
    10141 tgttctttta aggatgaaac ctttgttcta ctgtcttcga tatcggttgt ttcttgatag
    10201 tcttgggaag ctttctcaaa tgtcagctga tattctgact gcagataggt caaatgatta
    10261 tctaaaatca tttcagctcg gtctttttgg acttctaatt tggattgttc cgacagtcgt
    10321 gctttttgct cacgattacg ctctgccaag actgcttcaa gcttatcgat ctccttttgc
    10381 cttctatctc gctgctcttt ggcttttgtc aactcggctt ttaatgtttc tcgctgagca
    10441 gataattcgt taatttgttt ttccaaactt tcttctgtga cttcatgatc agaaaagtct
    10501 gctgtcaatg tcgccaactg cttttctatc gcttcttgac gttcggttgc ctctgctttt
    10561 tggactcggg caccgcggag ctggatctgc agatgattaa actgctcttt caagacagca
    10621 agatctgctt gttcttgtgc tttttgactt tgtacttgtg ctcttctggc ttccatctgg
    10681 tcgctttctt gacttaacga tttgatttct tcgtcgattt tttgtcgttg cgattcaaga
    10741 tctgtttgtt tgtctttcaa ttcgttcgtt tgcttttcgt aatcttcgat aaactgctgc
    10801 agttcacgtg tctcaaaagt tgagatttgc ttttcttttt caaaacgttc aagttcattt
    10861 atgatgtttt gtagctgatt tgtcgcttct tgttcttcaa aacgtaattg ttcgcctcgt
    10921 gtacgcaaaa cttcttgttc ttcggctaaa cgagctgttt cttgttgcag ctcctgaact
    10981 tttttttctt gtgcttggag ttgtttatct gcttcttcaa attcactagt caactgtttc
    11041 aattcttgat tttgtacaaa caaactgcct tggtttcctc gtttattcgc tccacctgtc
    11101 atggatcctc cggcattcat cacatcacct tctaaggaaa ccactcgata ttgatagcgg
    11161 atcgtttgcg cgattgcatt ggcactagtt aagtctttgg ctaaaaggat cgttcctagc
    11221 aggttttgaa ccacagtttg gatttgatcg ggataactga cttgttcact ggcaatccct
    11281 ataaaccctt ctacagctgc tgcttgtgtc aatatatgag ccggtaattg tcttggtttg
    11341 attgtcgtca gtggaaggaa tgttgctctt cctcctcttt gctgtttcaa atacgtgatg
    11401 gcttgccgag catctttttc attttcaacg atgacatgct gggctgcccc acctaaagct
    11461 gcttcgatag ctaacgtgaa atctgcagga acatcgatca gttcagccac tgctccgaca
    11521 atacccgaaa gctgctgttt atgctgcaag accaatcgaa ccccttggta aaaaccaaaa
    11581 tagttttctt ggatttcttg aaggcttttc ttacgagcac gtagctgctg gacttgattc
    11641 attaattgat acattttagg ctgttctttt gctaatgctt cttggaactt tttcccgttt
    11701 tgttggatca tttctaactt ctcttttgtt tcagttaaag atgattttag ctgcgcctgc
    11761 ttttgtgtca attcttcttt ttgtaagatc aatcggtcta ctgatgcttc tacttccgat
    11821 tgcttggcta acgtctgctt gctttttgct gtttcttgta tatattgacg ttctagatac
    11881 ttcagttcgt ttcctacagc tgcttgttct tgcatcaaat caacatattg atctcttaat
    11941 tctgccaata attctttcgt tgatttttgg tatttttcta attcttgctg tgtttttatg
    12001 agatttgctt cagctttttg gatctctgtt tctttttctg ctgctgcttt catcaagctt
    12061 tcttgaagct tttcaaaatg tttaaccttt ttttgtgctt ctgctaatga agtttgatat
    12121 tcttgcgagc ttttttgtgt atgctttgtc cgctcctgca aaacatcttt ttgtccttct
    12181 gtctgcttca gtttttccga aagatctaat aatacttgct gatttttttc gatcagacga
    12241 tcggcttgcg cattttcttt acgctgcttc gccaaaatgg attcttgttc ttggatcgac
    12301 tcagatagtt tgcctaattc tagattgaat ttcgcaagct gtgcttgttt gttgtcccaa
    12361 tctttttttg ctgtcttgat ttcagcaacc atcaaagaga catcggtttg agtcagtgtt
    12421 tcttttagcc gtaaaaactc ctttgctgcc tcactctggg cggccaaagg agttaactgt
    12481 tcttccaatt cgtggatgat gtcttggacg cggctgaggt tgtcttctgt ttcaaaaagc
    12541 ttctgttctg ctttcttttt ccgctgcttg tatttcaaga caccagctgc ttcttcaaag
    12601 atcccacgac ggtcttccgg tttactactg aaaatcgctt ctacttttcc ttgagaaatg
    12661 attgagaacg attcttttcc taatccagaa tctaagaaca attcttggat atcttttaat
    12721 ctgcagcttt gtttattgat gaaaaactcg ctttccccag tccgtcgata acggcgggtt
    12781 acactgatct cattgtactc caatggcaga taatgatcac tattgtctaa aatcactgtg
    12841 acttctgcaa tattcagctg cttacgggta tcggaaccgg caaagatgat atccggcatt
    12901 tttccgcctc gcagactttt agcagattgt tctcctaata cccagcggat agcctctgta
    12961 atattgcttt ttccgctgcc atttggtcca accactgctg tgacgctatt ttcaaaatca
    13021 atgactgtct tgtctgcaaa tgatttaaaa ccggctattt caattcgttt caaatacaaa
    13081 gctttccctc acttactttt acagtgctgc caatgcattt tccgccgcag cttgttctgc
    13141 taattttttc gatttccctt gtccagtgcc aatcaattga tcatctacat aaacttcgat
    13201 ccaaaatact cgttcatgag ctggaccttc ttcattgatc aatcggtatt cgattgatac
    13261 atcacctgat ttttgcaaga cttcttgtag ttttgtttta tgatccatct catgtgaaaa
    13321 agcacctgct ttgactttag gaaaaatcac tttttcgatg aatgcatggg tcgtgtcgaa
    13381 tccctggtca agatataacg cgcctaaaaa agcttcgaat aggtcgcaaa gcaatgctgg
    13441 tcgcgtacgt cctcctgaat tctcttctcc cttgcccaac aaaacatatt gatcaaagtg
    13501 acattctttg gcaaatttcg acaagctgtc ttcgcgaacg atcgcagctc gtgtttttgt
    13561 caattttcct tctggcaaat gaggatataa acggaataaa tactgagaaa ccatcagttc
    13621 taaaacggca tctcctaaaa attcaagtcg ctcattatcc gataattgca gattgcgatg
    13681 ctcattcaca taggatgaat gggtaaaagc ttgttcaagc aaatttagat cgtgaaaaac
    13741 gatgttatag ttttctttta gttcttttgt cagctggttg tccattttcc acattccttt
    13801 tatggatatt ttttcgatac ttccttatta tactggtttt ttgtcaaatt tgaaatggtt
    13861 cggtcaaaag gtaagtaaag ctttaataat cctagtgatt gacgagaagt tgtgaaaaat
    13921 tcgtttagtt tcgagtatga agaaaaaatt ttgtgaaaat ggctttccat atttttgcat
    13981 aaatttgact taataccgag aaactggact ttgaacaccg tttattggag aagtcatgaa
    14041 atcagcgcct tgacctgaat attagagaaa taaacagaaa aatatcaaaa aaccgaaaaa
    14101 ggacatcgcc ctttttcggt catttttagt gattatgaac gtgaatctgc tgtaacagaa
    14161 acatttgccc atgggtttgg tacatcataa gaccaatccc agtctttgac acggtcgttg
    14221 actggcaata cttcattacg gaacaacgtt ggaatcgcaa atgcttcttc aaatgcatat
    14281 tgttgccatt tttgatatgc ttctttttgg tactcttcat caaatgcttt gcttgaactg
    14341 atatcgttga tcaattttgt attttcttct gattcataac gagtgtagtt gaacgcagca
    14401 tttggtcccc atagtccact tggatttgga tcagttcctg tattccaagc agcttggtaa
    14461 acatcaattt caggatcatc gttttctaat ttatcataga aagaattgaa ttcgatcaga
    14521 cgacctgttg tgtattggac atccaaacca atttctttcc attgttggat atagtaatct
    14581 gccaatggtt gcgctgtttc cccaccggac atcgaagcaa agttgattgt tagtttattt
    14641 ccatctttgt cttctcttaa accatcgtca tccacatctt tgtagccagc atcatctaat
    14701 agttttttcg ctttgtctag atcttgtgtg aatcctttca tctcatcatc atggtatgac
    14761 tggaatactg gaggaatcaa tgaagtcgca ttgcttctca aacctgcata gaaacgttca
    14821 cctactgcat tattatctaa tgcatagccc attgcttgac gtaatgattt atcagccatt
    14881 tttgaatctg gattgtattc cacactcttt gtatctgcat tccatttacc taacttgaat
    14941 cctagataag tataagaagt ttgttcacgg cctaagtttg tatagccagc aatttcttta
    15001 taggtatcgt aagtatctgt tggcatttgg aaaatcatat catataattt agagttcaat
    15061 gcttcagttg cacttgccat cggtacagat ttcatcgtga tctttttcaa tttaggcgta
    15121 ccattccagt agtgctcgtt tggcaagtat tctactgatt ctcctgcaac gatattactc
    15181 atgtaataag ggccaaaagt cactggattt ttacgtactt gatcgcttga ttccatatct
    15241 ttgattgcta caccttctaa cgtatgtttt ggtgctgcat atgaccagat accaccaccc
    15301 gattgaagca tgcttgggtt tacttcttta tattcgattt caactgtttt gtcgtctatt
    15361 tttttgattc cggagattgt gtcggcactt ccatcatgat attcttccat acctacgatg
    15421 ttacggaaag tgtcgtcata acggatacca gtgtaatctt tattcccaat gatttcgtaa
    15481 gggaaaatca catcgtccgc cgttacatct tcaccatctg accatttgac gttgtctttt
    15541 actttgattg tcgctttttt cgtatcttca tctagatcaa gcgttgcagc tccgtcgtct
    15601 gtgattctga aatcgtcatc gtttgcaaat agcggttcat gagaaggagc cataaaagct
    15661 gcatcatatg aatcttggta aaactcccac tggaacaatc ctttaaactg actgtccgtt
    15721 gcgaccgcag cttctaattc gccgtcttcg attgcatcat cattattttt tgttgcaatt
    15781 ggaaaagtta attcctcttt gttattgccg ttgcctccag agttgtttcc agaggtatct
    15841 gtacctccgc ccccgcatgc tgcaagaacg acagcagaca tcagcgctgc aaatccaaaa
    15901 atcgttttct tcattcctat ttcccccttc gcttctatac tagcctaacc gttggcgcgc
    15961 atctgcagaa cgcttgaatg cttgtccaac ataattgata ctcaacatca aaactaaaat
    16021 aaggattgat gcgggtaacc agatccattg ccgattcact aagacttctc cgttacttgc
    16081 ataaccgatc aatgtaccta aactaggtac atttgccggc aagccgaaac ctaagaaagt
    16141 caaagttgtc tcgataccga tgtttgccgc aaagttcatc gtcaaattag taatgattaa
    16201 tgaacttaga ttcggcatga tttcgcggaa catgatttta aaatcattgg ttcccattgt
    16261 ttttgaggca ctgacataat ctcttcttgc ttcagataat gtcttgcttc ggaataatcg
    16321 ggctttagct acccaataaa atgcactcat gatgaatata aaggaccaga cattgtatct
    16381 ggaaataatc gatacgaaga cgataatgat cagcatgatc ggtagaatca tgataaaatc
    16441 aacgatccgc atcaagatgt tgtcaatgat ccctccgtag tagccagaga tgatccctaa
    16501 tccaaccccg atgatcgaag taaggatcgt gatcgcaaat ccgatcgtaa cagagtttct
    16561 agcacctatg atcaattgtc ctaatacatc acgtccgcct tcatctgctc ccagaatgaa
    16621 cttttgtcct tgtgtcgaaa ctgttcctgg tgcagcgtat ttgtcgaaga tactaacata
    16681 catcactttg tcttgatcgg tcagcatcgc accgataaag atcactaata ataaaaccac
    16741 tagcaaacct aatgaaaaaa tagcaatttt atctttttta aattctcgag caatcattcg
    16801 tatccccata ggaggaatac tttcttgaac tgctgtattg tttttttcac tcatcgatcc
    16861 attctccttt ctgattggtt ttattgaatc cgaatacgcg ggtcaacgat actcatgatg
    16921 atatctgaaa gtaatgtccc cactagcgtc gcgattccta aaatcaatac taaggatgtg
    16981 atcacggtat agtcccgttg gccgatggaa tcaataaata gttttccgac tcccggataa
    17041 ccaaaaatct tttcgataac tacagaacca gcaatcaacg aagtgatttc ataccccatt
    17101 tgagaagcca tcggcaacgc tgcatttcgg aaaatatgtc gactgaatac tttattcgtc
    17161 ggtacccctt tggaacgggc tgttctgaca aaatcgagcg atttcgaatc gattacttca
    17221 cttcgcagat attggattgt gacggcagtc gccaaaaatg cttgggtaat tgatggtaac
    17281 agaatatatt tcaaacggtc tatccattga gtgataaatc caccttgcaa acctagtgtt
    17341 tgtgatccac ttgttgggaa ccagcctaaa cgataaccaa aaacaaatag catgatcaag
    17401 ccgaagataa ataaaggaac agcaaaacta aagaagttgt agattacgac aatcttatcc
    17461 aagattgaat tttgataccg gccagcaagc atccccagag gcacagcaat caaataagta
    17521 aggacaactg tcaagatcga tagatataaa gtgttgatga tccgttcgcc gatcaagtca
    17581 gcaacagagc gtttgtataa gaaactttga ccaaaatcgc cttgcaaagc atttccgatc
    17641 caacggaagt actggacata ccatgggtca ttcaagccag ctgcttgtcg catctgttcg
    17701 atcactgccg gatcctgatt tggattgatc aaaccggtaa atggatcccc cggcatcgct
    17761 tgggctaata agaagatcag aacacttaaa ataataactt gcgggatcat caataaaaca
    17821 cgtcgcagaa tcgttttcca cattagcttg cacctccatc tttcaacgcc actttgtgtg
    17881 tagttgtgat cgtacgcaaa tcgtagactc gaccagtcgc atcatagtat tctttttggt
    17941 tttggatata ctcttgttct actcttcgtc gattttcttt gtggctttcc ctattttcta
    18001 catcgatttg cggaatagct gaaagcagac gtttcgtata gatatgtcgc gggtcattat
    18061 aaatgtcttc tcttgtaccg atttctacaa atcgaccttt gtacataatt gcgatattgt
    18121 cgcacatgtg ttttactaca cctaaatcat gtgaaatgaa taaatagctg aggccgtatt
    18181 cttgctggat tcgtttcatg aaattcaata cttgcgcttg aacagataga tccagagcag
    18241 atacaggttc atcggctacg atcagttttg gattggtagc aacagcacgg gctactccta
    18301 gacgttgacg ttgtccgcct gaaaattcat gaggatattt atatagcgca tcttccggca
    18361 tcccaacgat atctagcagt ccttttacct ttttcttttc ttcttgatcg cttaaccgtt
    18421 caaaatttcg gatgggttca gcaatgatat ccaacactcg ctttttagga ttcaaactgg
    18481 acattgaatc ttggaagatc atctgcacat ccttgttata acccattttt ttgcggttgc
    18541 ttcgcttcgt tacatctttt ccttcataca tgatttgtcc ggctgttacc ttttctaatc
    18601 ccacaacggc ttttcccgta gtagattttc cagagccaga ctctcccact aaaccatatg
    18661 tctttccttt ttcgataata aaatccacac catctacagc atatacatga tccgttactc
    18721 gattgaagaa tccactgcga atagggtaat gaactttcag gtctttgatt gtgattaatt
    18781 ctgtcatcta cacctctcct ttttcgtctc taaagtggaa atgcttatag caggagcaac
    18841 gtaccaagtg gttcggtgcg acttcatgaa ggataggctc ttcttcatga tcgcttgctg
    18901 gtatccatgg aatacgtggt gcaaaccgac aacctttacg aggcatgttt ttcagagaag
    18961 gaacgacacc ttcgatcaca tggagttctg catcatgatt tccttcttgt ggtatagagt
    19021 tcagtaaaga gcgtgtataa ggatgcttag gatgatcaaa cagttctttt acacctgcta
    19081 cttcaacgaa ctgtccgcca tacatcactg ccactcgatc agccatttct gctacgactc
    19141 ctaagtcatg agtgatcaag atgatccctg tttttgtttc ttcctgaagg tctttcagta
    19201 aatcaagaat ctgtgcttgg atggttacat ctagggctgt cgtcggttca tctgcaatga
    19261 cgattggagg tttgcaggcg attgcgattg cgatgatgac acgttgtctc ataccgcctg
    19321 atagttcatg gggatattgg cgtccaactc tcgcaggatt aggaatccct acttgttcta
    19381 gcaattctag tgcacgagct tgacgctgtt ctgcattcat ctttgtgtga taggaaagtc
    19441 cttctttgat ctggtcttcg attctcatga gaggatttaa tgcagacaac ggatcttgga
    19501 aaatcattcc gatatcattt ccgcggattt tattatagag cgtttcgttc aggtcgatta
    19561 aattcagatc gttatataaa atctcacctg taatttttgt gttattagga tcaagtaatc
    19621 ccataatcgt agtagccaag gtacttttcc cgcacccaga ttccccaacg attgctaaaa
    19681 tctcgttttt gtctaaagta aatgaaacgt catctactgc atcgtaatag tcatctttga
    19741 gtcggaatcc tgtatgtagg ttctgaactt ctaataattg gttatcggac aatcgaatac
    19801 ccctctctgc ttcctaaatg gaagtttttc ttaataaaaa aggacattac acagaataaa
    19861 tcgttttata acaaaggtta ttcttatctt tgtgtaatga a
//
LOCUS       DACESX010000045        19871 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.045, whole genome shotgun sequence.
ACCESSION   DACESX010000045 DACESX010000000
VERSION     DACESX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.045"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..661
                     /locus_tag="IXF88_001352"
     CDS             <1..661
                     /locus_tag="IXF88_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1508091.1"
                     /translation="LNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFV
                     YAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMG
                     IDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNS
                     GALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSAL
                     F"
     gene            673..1593
                     /locus_tag="IXF88_001353"
     CDS             673..1593
                     /locus_tag="IXF88_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1508092.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1634..3091
                     /locus_tag="IXF88_001354"
     CDS             1634..3091
                     /locus_tag="IXF88_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1508093.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3436..4050
                     /locus_tag="IXF88_001355"
     CDS             3436..4050
                     /locus_tag="IXF88_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1508094.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4043..5767
                     /locus_tag="IXF88_001356"
     CDS             4043..5767
                     /locus_tag="IXF88_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1508095.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5764..7215
                     /locus_tag="IXF88_001357"
     CDS             5764..7215
                     /locus_tag="IXF88_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1508096.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7688..9841)
                     /locus_tag="IXF88_001358"
     CDS             complement(7688..9841)
                     /locus_tag="IXF88_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1508097.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9843..10889)
                     /locus_tag="IXF88_001359"
     CDS             complement(9843..10889)
                     /locus_tag="IXF88_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1508098.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11039..12325
                     /locus_tag="IXF88_001360"
     CDS             11039..12325
                     /locus_tag="IXF88_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1508099.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVE"
     gene            12341..15043
                     /locus_tag="IXF88_001361"
     CDS             12341..15043
                     /locus_tag="IXF88_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1508100.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15028..15885
                     /locus_tag="IXF88_001362"
     CDS             15028..15885
                     /locus_tag="IXF88_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1508101.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15956..16813)
                     /locus_tag="IXF88_001363"
     CDS             complement(15956..16813)
                     /locus_tag="IXF88_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1508102.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            17024..19195
                     /locus_tag="IXF88_001364"
     CDS             17024..19195
                     /locus_tag="IXF88_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1508103.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19263..19604
                     /locus_tag="IXF88_001365"
     CDS             19263..19604
                     /locus_tag="IXF88_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1508104.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
BASE COUNT         6648 a         3520 c         4100 g         5603 t
ORIGIN      
        1 tttgaatctg ctgatctcag tcgtatttgc aatcattatg agcgagttga gaaataaacg
       61 actcgttaaa gtttatcaaa cgatgtcctt actaccttat tttctatctt gggtcattat
      121 cagttacttt gtttacgctt ttttaagtcc tgacaaagga atattcaatc agtggatcac
      181 tggacatgga ggagaagctg tcaattggta taatgaaccg aaatattggc cgtttatctt
      241 ggtgttcatt ggtacatgga aagggattgg ttataacagc attatttact ttgcatctgt
      301 tatgggaatc gatccgactt attatgaagc tgctatggtt gatggagcta gcaaatggca
      361 gcagatcaaa catgtgacga taccacaact tattccacta atgacgattt taacgatttt
      421 agccgtagga aacattttcc gagctgattt tggcctgttt tataacattc ctaggaattc
      481 cggtgcatta tatgatgtga ctcaagtttt ggatacgtat atctacaatg gcctgacatc
      541 cacaggagat tttggtatga cggctgctgc aggactttac caatcagttg tcggctttgt
      601 cttgttgatg atcacaaata cgatcgcccg acgttttgat agcgattctg cattattcta
      661 aggaggaaaa aaatgaaaaa aaagaaagtg gagagagtcc aagtccgctc tttcaataag
      721 acggctaatt ttttctctaa tataatgatt gcactctttg ctatttcctg tattttgcca
      781 tttatatttg ttatcgccat ctcttttacc gatgagtcca tattgtcgac tcacgggtac
      841 agtttttggc cagaaaagtt cagtatattt ggttatacgt acttgttcga ccagatgcag
      901 gataaaattt tccaagcgct tttcgtcact gtacttgtta ctgttttagg tacactgatc
      961 aatagcacgg ctacttcttt atatgcttat gcgatttcac ggtcaaactt tccattccgt
     1021 cgttttttta ccgttttttg tttgattacg atgttgttct caccagggat ggtggccaat
     1081 tatcttgtta tgacaaatct tcttcagtta aaagatacga tttgggcatt gatcttgcca
     1141 atggctgtca gtcccttcaa tattattgtg atgcgcacat tctttaaacg ttcggtctct
     1201 gattcgatta ttgaatcggc acggatcgat ggagctagcg aactgcgaat atttgttcag
     1261 atcgttttac cgttagctgt tccaggtatt gcgacaatca gtttatttgc cgcattgggt
     1321 tactggaatg actggttcaa tgctttacta tacatccaag atgataagct agttccacta
     1381 cagtatcttt tgatgaaaat ccaaagtaat attcaatatc tgacacaaaa ttcaggagca
     1441 ggaagccaat tgacaggcgg attagcctca gttccaggag aatctgccag aatggcgatc
     1501 gtagtcatct cgacgttgcc tattgcgatc agctatccat ttttccagaa gcattttgtc
     1561 aaaggtctaa caatcggcgg agtaaaagaa taaagaaaca aaaatataaa tcataaaatt
     1621 aggaggaaga aaaatgaagt tatggaagaa actagccttt acaggtgttt cagcgttgtt
     1681 gttgggaaca ttagcagcat gcggaggttc tggttctaaa gaccaggcag aagctagcaa
     1741 ttcggacact ttgcagatgt atcaaatcgg agataaacca gataatttcg atcaattgat
     1801 ggaagtagca aacaaacgaa tcgaaaaaga gatcggtgta aaagtcaata tcaattacat
     1861 tggctggggc gattacgaaa agaaaatgaa cgtcatcatt tcttccgggg aaaattatga
     1921 tatcgccttt gcgaacaact atgtttccaa tgcccaaaaa ggagcctttg ctgatttgac
     1981 tgaattagcg cctaagtacg ctgaaaaagc ttataatgat ttagatgaag catatattaa
     2041 aggaaatctg gtcaatggaa aattatatgc tttcccagtc aatggaaatg tctttgctca
     2101 acaagtactt acttttaaca aagctttact agataaatac gatctttcaa ttgatggaat
     2161 ccaatcttac gctgatgcag aaaaagtctt gaaggaattc catgaaaaag aaccaaacac
     2221 ggcagctttt gccattggac aaggcttcaa agtccaaggg aactttgatt atcctttagg
     2281 gaatacgtta ccatttgcta ttgatctgga tggagatgat tcgaaaatca tcaaccaata
     2341 cgataatgaa acgtatatcg gactgttacg tacaatgcat agctggtaca aacaaggact
     2401 cattccaagt gacgctgcaa cgagcaataa ggattatcca ctagaaggaa atacttggct
     2461 aatgcgtgga gaaacacaag gaccgtatga ttatggcgat acaattttga caaatgcagc
     2521 aaaacaagaa cttgtctcaa aagcaattac tgttccacta aaatctacag ctcaagcaca
     2581 aatggcaaat tttgtcgtat caaatacttc aaaaaataaa gaaaaatcag tagaacttct
     2641 agggctgctt aacagtgatc cagaattatt gaatgggctt gtttggggga tcgagggaga
     2701 agcatgggaa aaagtcgatg gttcagatga taaaatcaaa ttgctagatg gttatcagcc
     2761 aaatacacat atgtctgctt ggaatacagg aaacaacaag attctatata cacaagagtc
     2821 tattacggat gacatgattg ccaaacgtga tcaatcgatt gctgatgcag aaacttcacc
     2881 gattttagga ttcagcttca acactgacag tgtcaaaaca gaattaagta atatttctaa
     2941 tgtcatgaat caatatttag atggattgaa tacagggact gttgatccag atgaaacatt
     3001 accgaaattg aaagacgctt tggacaaggc tggctatgat aaggtattga aagaaatgca
     3061 aaaacaatat gatgagttcc gacaagaata ataatgaata aagacaaagg ggttgtgaca
     3121 aaagacgtgt cacaacctct tgttccgaat aaacggtgtt caaaagccag tttctcggta
     3181 ttaagctaaa tatatgtaaa aatgtggaaa gccatattca caaaattttt cttactactc
     3241 gaaactaagc ggctatttca caaactcttg gtaaacggtg ttcaatcagc taaccctcgg
     3301 tattcgctca caaaacgaca aaatatgagg aactattttt ccgtttgttt ctctaataat
     3361 caggtcaagg tgctgattgc ccaacctttt tttgtataat gagaaagtat aaacttaatg
     3421 tttggaggag aagatatgtt agggaaggtt ttggaagagt tatttatccg gatctgggtc
     3481 attataaaac taaccttgta tttctggata tatacatttg ccggcgggat catctttgga
     3541 ttaggtgcgg catggaaaac agtcaatgaa ctattttatt tgtacggatt cgagtataaa
     3601 gaaatcacaa taaaaagagg ctggaatata tataaaagaa attttttaag aggcaatctg
     3661 ctatttagtt tatttttatc tggtactgct ttattgagtt acaatttata tttatctgtt
     3721 caaatcaaag ggctcatttt tttagctatt gattttattt tactttttgc cttgctctgc
     3781 ctatttgcta cttatcaata cagtatgatt ttagatagtg aatatacgat ctctgttcct
     3841 aatctgctaa agctaagttt tatttcggtt ttttccagct tttcgtcttt tctaaaaata
     3901 attattggta gcggtataat tttaggcgtg acttggcaat tcaaaggatt gatcctgttt
     3961 ggtgtcattg gtcttttgac ggtttggaat ggtacgatga caacacattg gcgtgaagaa
     4021 ttagataaac agttggaaag ttatgaataa gtttcggatt ctattttcaa aaggaacctt
     4081 actaaatcgt ctgttgcgga aatacgcggt tatcatgatc ggtgtaacga tgactgctac
     4141 cgttattttt agtatccata cttgggaaca aaatcaaaaa caggcagaaa acatgacaag
     4201 tgatgccgtt caaagcacaa gcagaatgct gaatgataaa acaaccttga gtaggatcat
     4261 caaaaaccag cttgtaggga attccgaaaa gatcgaaaat gtgacgacct acttaacaaa
     4321 acccatagat cagtatctga tgtatgtata cgaacagcaa aacagtaccg atgaattagt
     4381 ttctttccca aatcaaatca aagatctcta cgcaaattat gaagaactaa gtgcgatata
     4441 tatcgtgttg aatcaactac cggagtatta tgaatcttct agagaaaaca aaggtgggga
     4501 aataaaaaag gggacaccaa aattactaga cgccttttat atcaagttgc ctatcacaca
     4561 aggaggagct gagtcaggaa gtatttttat tggttttcaa aagaaagaac tagatatgat
     4621 tttagaaaat ctaacgtcat tcaatggact ctctttatac atgatttctg gaacgacaaa
     4681 tcgactatac acattccatg ataataaaat agctaaagaa acgtttgaaa gacaagaaat
     4741 gatcatcaca gagagtctga agaaaaattc acaattgccg attgacgatc tggaaaaaaa
     4801 caattttgtt caacaccaag aactgtctga tgattatcgt atattagctg tactagataa
     4861 aaaaagtgta aaaatcgaaa cgggaaaaaa tcttgccccg ctgatcattg gtatcattat
     4921 tttagacaat atactgctta tctttcttta taaaacattc aaacgatatt cacagcaagt
     4981 atctatcgtc atgcaggcca tggatcaaac agcagcaggg aatttagaga cgcggatcga
     5041 cataacaaac acggaatatg aactgaagga actttcaatc ggtatcaacg aaatgttgga
     5101 tagcatcaat cagtttgtag aagacatcta caaattagaa atcaaacagc aagacgcaca
     5161 tatgcgggcg cttcaagcac agatcaatcc acacttttta tataatacat tagaatatat
     5221 ccgaatgtat gcaatcagcg aaggaagtga agaattagca gatgttgtgt atgcgttttc
     5281 cgctttgctt cgaaacaata cgaatcaaga gaaaacgatc actttaaaag aagaactgga
     5341 cttttgcgaa aaatatgtct atttgtatca aatgcgttat cctaatcgag tagcctatca
     5401 ttttatgatc gatcctgatt tggaaaagat cgaagtacct aaatttgtga tccagccatt
     5461 agttgaaaat tattttaaac acggtattga ttttactcga ttcgataatg cgctaagtgt
     5521 caaagtcttg caggaaggca aacgggtaag gatcatcatc aaagataatg gaaaagggat
     5581 gactgaaaaa cggctaaaac agattgaaga aaaactgtct catcctaaag tggaacttca
     5641 cggttctatc ggtctgcaaa acgtgaatga acgtttacgt gccagcttcg gcagcagtta
     5701 ttatatgtcg cttgagaata atgagacagg tggactgacc gtttctatta cattcaagga
     5761 gggatgagcg tgtatcaagt gttattggta gatgatgaat acatgatttt gaatgggtta
     5821 aaaaagataa ttgattggca aagtttaggc tttcaaatag tagcaacagc agagaatgcc
     5881 aaagaaggac tagctgtact tgaacagcga cagattgatt tagtagtaac ggatgtgaca
     5941 atgccagaaa taaatggact ggaatttatt gaagcggctc aaaaagaacg tcataatttt
     6001 gaatttatga ttttatcagg ataccaagaa ttcgattatt tgaaaggagg tatgcaatta
     6061 ggcgccgtga attatttgat gaaacccgtg aataagttcg aattggttga aagcctcaag
     6121 aaaatcaaaa cacggttaga tcagcaaaat gaacaaaaaa atcaacaaga aatctatcaa
     6181 gaactgttat tcagtcaatg gttgaatgaa gaattggatg aaataagtga agaagaaata
     6241 ataggacaaa taagtgagaa gcagcatagg atagtattga ttcaactggc aagaactcat
     6301 gatactctta tcaatagatg gttgcaagaa cggcaacaaa tcttttacta tcaacgaaat
     6361 tacggagacc gtgtattgat cacgttgttg ttggaggcag atgaagttga tggcttctgt
     6421 tgttttgttc aagaaaactt tcctgatcag gaatggctga tcagtatcgg ggaagagaca
     6481 gattctttgg acaaaatccc tgaaacattc caacaagcga aagacaatct acagttgcat
     6541 caattttatg gagataaaga cgagcatgtt ttttacgcag tacaagcaga aacaaaagat
     6601 caaaccatcg atttttcaag tttcagacgc gtgctgcaaa ataaacaatt ggaagtcgct
     6661 caaaaaatga tcactgattt ttttgaacag tttcagttag cagccatgat gccggaagat
     6721 atacgatatt ccagtttttt actgtttatg gagattcaac gggaattgat tgatctggaa
     6781 gatgaggaat atttacaagg aatcgaaaaa atccatcagg caaaaacggt gcaagaactt
     6841 cagcagctac ttctgtcatt catcaaaaag catcagagac agaaaaagta cagtgacaat
     6901 gtggaaaaag taatcgtaat cctgcaccag cattatcaag aaccacttac actcaaagaa
     6961 gtgtcagaaa acctgcattt aaatgtcatg tatctaggac agctgttcaa aaaagaaacg
     7021 aaaaaaagtt tttcagctta tttgaaccat ttacgtatgg aaaaagcgaa acaactgctt
     7081 ttgcacagta atcaaaacat caatgagata gcaagtgaga ttggctacaa caacaccacg
     7141 tatttctcta aattattcaa gaaaatcgtc ggcagatccc ctaaagagta ccgagaaaac
     7201 agtggagaat cataaaagaa aaagccaatg taatgaaata acttataaaa aattgtctag
     7261 aaatctaatt ttgggctgtt cttattactt gaggcaaggc agctttttca tgacctctcg
     7321 ataaacggag ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtgga
     7381 aagccatttt cacaaaattt ttcttaatac tcgaaactaa acagcttttt cacgacctct
     7441 ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaatg gcaaaatatg
     7501 aggaactatt ttgccatttg tctctctaat actcaggtca gaacgctgat ttcacaacct
     7561 ctcaataaac ggtgttcaaa agcttgcatc tgcggtaata agaccaacca aacgaaaaat
     7621 atgaggatct atttttgctt ggctgttctt attacttgat gcaggacagc tttttcacaa
     7681 cctcttgtta attatagttt gataaggaaa aaggcaaatc ttttgcttga tatttcttag
     7741 ggtgagggat gatccctaat tggtaagaga tcgtgccgcc tttcagcaaa tcttgatggg
     7801 taaaatataa tttcgtatat tctttgtcca tccgtttgat tttatggata aattgttgct
     7861 gcggccgatt aggcgttgct tcaatcgtca attcctttcc atttgacaaa agcaaaacag
     7921 ccttgtcaat taatggcata ccgataacat attcaccggt tccaggcgtg acaggataaa
     7981 atcccagact attgaagata taccaagcgg ccatacttcc attgtcctca tctccagggt
     8041 aacccattgg agaatcgtcg aaggtctgag tcatcaactg tttaagcagt ggctgtgcca
     8101 tttcaggttg tcctatataa ctgaacaagt aaggatagtg aaaacttggc tggtttgaaa
     8161 tagctaattg tccaaattct atcgcggcca tctcactcat ttcgtggatc tcaaaaccat
     8221 atcctcctgg gtcaaacgtt gggggctgat tgcacagctc aatgagtttt tctttatatc
     8281 tctcactgcc accatatttt ccgatcagtc ctgaaaaatc gtgataaaca gcaaagctgc
     8341 tttgccaagc acttccttct gcataatctt gtccccaacg attagctgaa aatggttctc
     8401 tgaacttgcc atctttatct tttgaacgca taaatcctga ctcatagtca aagatatttt
     8461 gatagttcaa tgattgctgc tgatatttct tcgtcatttc ctcttcttct agtaccttgg
     8521 caacttggct aatacaaaaa tcgctataag catagtctaa tgtgtgattg actgattcgt
     8581 ggtagtgatt aggaacatag ccatatgtca agtaatcttc tgttccttgg cgtccataat
     8641 ttggatgttt gctttgaacc gtagcacctt tttccattgc cgttagaaat tcaggcatca
     8701 agtctcgtcg gatatctttt acagcagcat ctgcaatgac cgcatcgatc aatgtcccag
     8761 gcataagtcc acgttcatca ggagacagcc attttggcaa atagccactt tctctatagc
     8821 tgttcaaaaa accttccagc atttcttcgt atttttctac agcaatcaaa gaataaagag
     8881 gataaaccgt tttatacgta tcccaaaacc cattatttgt atagagaaca ccaggtttta
     8941 tagatcgact agtcgtatca tagtggattt tttgcccact aggatcaatt tcataaaatg
     9001 tttgtggaaa taaaaacacc cgatataaat tatgataaaa cgtccgcaat tgttgttgat
     9061 catggtgtgt gagctgtata cgatcaaaat aggactgcca ttgttcttga ctgtgagcaa
     9121 ggtaagtctc tttgctttgg ttttgttcta gttccaggtt ataagctgct tgttccgagc
     9181 tgataaaaga tgtcccaaat cggatagttt gtttttttgt ctgttcaaaa tacaacggga
     9241 tcgagtcgct atttttgttg gaaacagcta actcatcaat tgttaaagga tattcaaatc
     9301 tcaatataaa ataaaaagaa aaatcaggat cttctgaacc agcaaaatgt gtgatttttc
     9361 cttccactgt gtaaggatct ataacaaaga gctgatggcg tcccggaaag gataaaagta
     9421 aaccgctgtc atttttgctg taatcaatag ataagattcc gccatacatg ctaggtatca
     9481 atgtagaaca gatgccataa cgtaattgac agatcgacag atgagtcggg caaaatatac
     9541 tttcttctgg gcggtaagaa ctttgagcat ggaaaagggt gttctcctgt agttttccat
     9601 tgatcggcgt aaacagcata tgactgaaat cacccatcca aggacttggt tgatgggtaa
     9661 gacggtagcc ttgaaaaacg cggtcatcag gatgaaacca ccagctgccg ttttgatccg
     9721 tagtttgcgg tgcaaaataa ttcatcccaa aaggaacgcc agtataaggc tggcagttcc
     9781 cattagagaa atttggctga ttcgctgttg catgtctagt atctatttga ccgatattca
     9841 ttttatttct cctttgttga ttgtcttttg atgaatgaaa ctggaaattt actatcggat
     9901 ggtcgatctt ttgtctctaa ccaagtgacc aaagcatctc ccgcaagctt acctaactgt
     9961 tcaaaatctt gtgcgatggt tgtcagtggc gggtcaacaa gagctgctgc ttggatatca
    10021 tcaaatccga taattgaaaa atcttcaggg atatgataac catgctgtct caagagattc
    10081 atggtctgga tagcgacaac atcgttttca caaacaaaag cagttacttc atacttagtg
    10141 atataagaaa gcaattcatc taatgtcgcg cttttgtcat ccagtgaagt gtggaaagtc
    10201 aaaccagctt tgtccaatgc ttcaaggtaa ccaaggtaac gctgtcgaac ggattgtgga
    10261 tgagtctgcg taccaaacaa ataagcgatt tttgaatgtc cctgttcgat caaataagag
    10321 gcagcaagcg caccgccttg gaaattatcg gaaagaacgg taggaaaagg taactcatag
    10381 atttttttat ctaatatgac gacagggaat tctttcaatg aaagctcaaa aagtaagtca
    10441 agatgttgtt ctgtatcaag agcataataa atcaatccgt caaattcatt gattagctct
    10501 tttgctgttt tattttgtag aaagtcaagc gtcgtcatca tgacatcaat ctgtttatct
    10561 tgcatcacag ggttcaatcc cttgctaaaa tcaccaacag acaaatcagc gacatatgga
    10621 agaagaaaca gaatccgtcg tgtttttgta ggagaagcaa gaatctgttc cggagaagcg
    10681 gatttaacga aactgccttt cccgcgaacg cgataaataa gtcctaattg ctctaattca
    10741 gtcaatgcac gtttggaagt gattctgctt actttatagg tacgtgaaag ttctttttca
    10801 gttggtagtt ggctgtctac aggtaattca ccagttaaaa tagccgtttg caaatcatta
    10861 aatattgtct ggtacaatgg ttgattcata taaacacctc ttaatgatat atcatattca
    10921 aataatacca taatggatga aaaagtaaat aaaaagatat tttttgaaag cgtagacata
    10981 attggctgta tgaagtataa tctttaaaga tatatcaaat agaaagaagg ttttattagt
    11041 ggcttataaa gatataccaa aatcggtgca gaaatttatg gaagatatca cacaaaaatg
    11101 tggggatatc catgcggact gggcaaagaa ttttacagca gcatttgcca atactctgtt
    11161 gacaacagtc aaaaagcaaa atgacggaac caccttttta ttgacgggag acattccagc
    11221 gatgtggtta agagactcta ctgcacaagt tcgtccttat ttagtgattg caaaagaaga
    11281 tgaggaatta gctgacatga tcagcgggtt agtcaaaaag caattttttt atatcaatat
    11341 cgatccatat gcgaatgcct tcaacgaaga agctaacggt gctggccacc aaacagatca
    11401 tacaaaaatg aacgattgga tctgggaaag aaaatatgaa attgattctt tatgctaccc
    11461 aatacagttg gcctatcttc tttacaaaaa tacaggaaaa actgatcaat tcaatcaatc
    11521 ttttattgaa ggtatcaaaa aaatcttgga cgtgttcaaa acagagcaag atcatgaaca
    11581 ctctccttat acatttgtac gagacacgac aagaaaagaa gatacgctga tcaatgacgg
    11641 gcgaggtgca aaaacagctt ttacaggaat gacatggtct ggtttccgtc ctagcgatga
    11701 tacttgccgt tatggatatc tggtaccatc caatatgttt gcggtggtcg tgttaggtta
    11761 tgtggaagaa atttttcaaa cgatactcga tgataaagaa attgtacagc aaacacagaa
    11821 attgaagaaa gagatccaag aggggattca gaatcacgga cgaacggtta atcaaaaagg
    11881 gcaaacgatt tatgcatatg aagtagatgg aatgggaaac gccacagtca tggatgacag
    11941 taatgtgccc aatctgatcg ccgcacctta cttgggatat tgttctgaac aagaggaaga
    12001 ttatctgcaa acaagaagaa cattgttgag taaagaaaat ccatatttct atgaaggaaa
    12061 gtacgcaaaa ggaattggat cctcccatac gccagaaaaa tacgtttggc cgattgcatt
    12121 agctatggaa ggaatgacaa cgtcagacaa atctgaaaaa gagcggatat tgaatttact
    12181 tgtagcaact gacgcaggaa cacaccttat gcacgaagga ttcgatgtga acaatcctga
    12241 aaattatacg agagaatggt tttcttgggc aaatatgatg ttttgcgaat tagtcatgga
    12301 ttactttgat attagagtag aatagtagga ggtatcgtaa atgaaaaaga aagtatatat
    12361 catttctcat agccactggg atcgtgaatg gtacatggct tacgaacagc atcacatgcg
    12421 actgattgaa ttgatagacg atctactaga gctatttgaa gttgatccgt ccttcaacag
    12481 ttttcatttg gatgggcaaa cgattatttt agatgactat cttcaagtaa ggccagaaaa
    12541 aagacaggca atccaacaag caatcaatga aggaaaattg agaattggac cattttatat
    12601 cttgcaagat gattttctta tcagctcgga atcgaacgtg agaaatatgc tgattggcat
    12661 ggaagaaagc cgtaaatggg gaacccctgt catgttaggc tattttcccg atacatttgg
    12721 aaatatggga cagacgcctc aattaatgaa acaagctggt atttcagcag cagcgtttgg
    12781 ccgtggtgta aaaccaattg gctttgataa tcaagttttg gaagcagaaa actattcttc
    12841 ccaatactca gagatgtggt ggaaaggacc tgatcaaaca gcaatctttg gtctgctatt
    12901 tgccaattgg tatagcaacg ggaatgagat tccagtagaa aaagaagcag ctcttgcctt
    12961 ttggaagcaa aaattagcgg atgcagaaca atatgcctct acgaaccact tattgatgat
    13021 gaatggtgtt gatcatcaac cggtacaaaa agatatatca aaagcaatcc atctcgcaaa
    13081 tgaactgttt ccagattacg agtttattca ttctaatttt actgactatc tggaagcagt
    13141 tcaaaaagat gtgccagaag acttaggctc agtagaagga gaattgacaa gtcaagaaac
    13201 ggatggctgg tatactttag ccaatacagc atctgctcgg gtttacttaa aacaatggaa
    13261 taccaaggtc cagcgacagc tagaaaatat tacagaacca cttgcgacga tggcttatga
    13321 agttagtgga aactatcccc atgatcaatt agattatgct tggaagacat taatgcaaaa
    13381 tcatcctcac gacagtattt gcggttgttc agtcgattct gtccatcggg aaatgatacc
    13441 tcgttttgaa aaagcagatg aagttggaaa gtatcttgca caagattcct tagaacagct
    13501 gaccgcagca atcgatacga ccggattccc aaaagacagc tttccatttg tcattgtaaa
    13561 tactgcagga atggataaaa ctggtgaagc agaaataacc atcgaattag aaagaaaaag
    13621 attcgctgaa ggaatacctg aacaactata tcaagagtta gaaaatttgc ctaaacgtaa
    13681 gtatcatgta gaaacaaaaa gtggggccac tataccagct attttatctg aagaaactgt
    13741 ccaatttggt tatgatttgc cgaaagaccg tttccgtgtg ccatatatgg caagaatgat
    13801 caaagtgact ttaccattag aaaacatgcc agcattttct tgggaaacat tcgcgcttgt
    13861 agaaggggaa gcgaaagcag aagaaaagga aacaatgatt catcagagtg gacgaatcat
    13921 cgaaaacggc cctctacatt tgacgattga aaaaaatggt accattacaa tggaagatcg
    13981 caaaaacaaa cgaaagttga ataatctgca tatttttgaa gatatcggcg atataggaaa
    14041 tgagtatatc tttaaacaac ctttctgtga tcagccaatc ctatccagta acaaagaaaa
    14101 ctctgaagta aaagtattag tagatacacc ggaaatagca aaaataagta ttctacagga
    14161 aatggaaatc cctgtttctg cagacgagcg tttagaaaaa gaacagcaga tggttgtgga
    14221 atttcgttat cgaaaagcag aacgatccaa agaaaaacga atccttcaaa taaaaacaat
    14281 catgacaatc agaaaagata gtaaaaaaat cgactttgaa acgactctcg acaatcagat
    14341 gaaagatcat cgcttacgtg tcttgtttcc tacaaagtta catgtggaac atcacgaagc
    14401 agatagtatt tttgaagtgg tgaagcgccc aaaccatgtg tctaaaagtt gggaaaaccc
    14461 cacgaatccg caacatcagc aagcatttgt caatattcat gatgaagagt acggtgtaac
    14521 agtcggaaac tttggtttga atgaatatga agtaacagaa gacggacaaa tcgcagtgac
    14581 gctacttcgc agtgtgggag aactaggcga ttggggatat tttccaacac cagaagcaca
    14641 gtgtctaggt gagcatcggt tcaattacag cattgaacta catggaccag aagaaaaatt
    14701 ctctacttat ttgcatgcat acgcagcaca gattcctttt tccactcaac aaatcaaaca
    14761 tcatgaagga acattgattt caaaacaaca atatttaacg ataaaaagtg aaacgtttgc
    14821 aattacagct ttgaaacgca gcaagtttag tgataaagta gtagtaagag gtttcaatat
    14881 gtctagtcat ttagaaaaat tggaaataac taaagacaat ggaaaaactg tcatattgaa
    14941 tctgcttgaa gaaccaacga agcaagcggt tgtcccaata atccaacctt atgagattcg
    15001 gacaatcgga ttcgaggagg aaaacgaatg tatggcggaa tagaagcagg cggaacaaaa
    15061 tttgtgtgtg cggtttcaga tggtggagaa attttagaaa aattaagtat ccctaccaca
    15121 acaccagaag aaacattgga tcaggtattt gaattttttg atcgttatga attgaaggga
    15181 atcgggattg gctcattcgg cccgattggt atcgacaaaa caaaagataa ttacggatac
    15241 gttttggcaa ctcccaaaaa agggtgggca caattcgatt ttcttggcac aatcaaggaa
    15301 cgctacaata ttcctgctgc ttggacaacg gatgtcaatg ctgctgcata tggggaattg
    15361 aagcaaggag cagcacaagg aaaaaatagt tgtatttacc ttaccgtggg aacaggaatc
    15421 ggtgcaggtg tcgtgatcaa tgaagaaatt ttttcaggga tcgcacatcc tgaaatggga
    15481 catatttggg tgaaacgcca tccagatgac cattatgaag gtacttgtcc ttatcataaa
    15541 gattgtttag aaggattagc agcaggaccg tctattgagg cacgcacagg gatcaaaggg
    15601 caagatctgc cgcaagatca tcctgtttgg gctattcagg cttattatat tgctcaagca
    15661 ctgatcaact atacgttgac gttagcacct gaaaagatta ttttaggcgg tggggtaatg
    15721 aatcaggatc atctccttca aaaaatccgt cagcaatttg ttgaattgat gggtggttat
    15781 atggaaacac ctcaagtaag tgaatatatc gttcgttggg gactgccgaa tgaaagtggg
    15841 attatcggta gtttgctgtt agcagaacac gaacatcaaa agtaagcaga caaattacac
    15901 aaaaagaaca gagtcttatc attatctgct gataagactc tgttcttttt tatttctatt
    15961 cccataatcc aggtaaagct ttaccgacta ttttttcttg agtaaatgga tggatcatgt
    16021 gcagttctaa tgcatggagc atcagtcgtt cagctgttct attttggtag agagggtctc
    16081 caacgatcgg atgtccctta tgagctaaat gaactctgat ctggtgtgta cgtccggttt
    16141 ccaaaacaca atagacttgt gtttgttcag aaagtacttt gttgacagtg acatgtgtca
    16201 aggcgctctg accattttta tcatctacga ccctttttct ccgatcatgg cgatctcgtc
    16261 caattttatt ggtgatggtc atatctttgg caatttctcc ccatacgatg gcttgatatc
    16321 tccgatagat cattttttgt tccaacattc ttccaagtat cggcaagaca aatggatttt
    16381 ttgcaaataa aacggcaccg cttgtttctt tgtccaagcg gtggacaacg taaggtcgtt
    16441 gctgtttagt gtcgagataa gcagctaaat gattgagtaa ggtactgttt tctgtcggtt
    16501 catttggatg agtcttcatt cctgcaggct tatccactat aatcaaatgc tcatcttcat
    16561 ataaaacacg gatgtgcttc gcatctccta attgaacttc ttgataatta taatctgttt
    16621 cttcgaataa caatgtgatc tggtcattgc cctttacttc atgatggaac aaagcaggtt
    16681 gttgattgat ccaaacgttt ttacgtatac gcaaaaagtg tcgaaccttt ctaggaacta
    16741 accattcctt ttcaagcaag tcgcgaattg ttgtttcagg atggttcata ggtaaagtga
    16801 tcgtgatttt cacaagtgta ctcctttcag tgcaatcgca gactaattgt accattttta
    16861 aaacatattt aagaaaaaac ttacattttt ctgttcattc ttatgttact ctacttgcac
    16921 taacgtaaaa aaacatattg aactacaact taagcaggaa gaaaacactc tttcactatg
    16981 gtaaaataag acgattgaat caaatagaca ggagaagaag caaatggatt ttcaaagcat
    17041 tttagaaaag ataaaagctg ctttgatcag tttttggcgt tggatcagac catatctcgg
    17101 acaattccat cgatggcgaa agcgcatctg gaaaaaatat catgtgaaca aattgatttt
    17161 gctgattggt ttgatctgtg tcttagtgac aagtatctac ctctttattt tagcaaaaca
    17221 agcaaatgtc gaaacgttaa aatcaggatt aagccaatcg acagtcattt atgataagaa
    17281 aaacgaagaa gctggcacac tgtacgccca aaaaggaaca tatgtagaat taaacgcgat
    17341 ttctccctat atccaagatg cagtaatctc gacagaagat aggaattttt acgagcatca
    17401 cgggtttgat atcaaaggga ttgcccgtgc agcagtccga atggtcttgt ctggcggaac
    17461 atcagggggc ggaggaagta cgatcactca gcagcttgcc aaaaatgcgt atctgacatt
    17521 agaccaaacg tttgatcgaa aagcgaagga attatttttg gcaatcgaga tcgaaaagaa
    17581 gtacagcaaa gaagaaatct tgaccatgta tcttaacaat gcctattttg gcaacggggt
    17641 atggggtgtc caagacgctt ccagaaagta ttttggagta gatgcaacga atgttacgct
    17701 gtctgaggct gctactttgg caggaatgct gaaaggtcct ggtatttata atccgatcga
    17761 tcacccaaaa aatgcgaatg atcggaaaaa cacagtacta agtgtaatgg aagaaaacgg
    17821 aaaaatcaca aaagaacaag aacaagcaga aagccagaca gatatcagcg cgtatcttaa
    17881 tgacacatac gataattcag attctggtta tcgttatcct tattattttg atgcagtgat
    17941 cgatgaagca gttaacgagt atggtcttga tgaagaagat gtcatgaata aaggctataa
    18001 aatctataca gctttagatc aggattatca aaagcaatta gaagctactt atcaaaacag
    18061 tgcattattc ccagcaaatg cttcagatgg tgccatggtc caatctgctt cagtagcttt
    18121 agatccgaat acaggtggtg tacaagcact agtaggacga agaggagatc atgtctttag
    18181 aggattcagc tttgctacac agatgcgtcg ttcaccagga tcaacgatca aaccattaag
    18241 cgtatataca ccagcactgg aagctggata caagccttcc agtatcttga aagacgaacc
    18301 gcaaagttat tacgatgcgc ataattttga cgggacatac caaggagaag taccgatgta
    18361 tcaagctgtt gcacaaagtt tgaatttgcc tactgtatgg cttttgcatg agatcggttt
    18421 acaaaaagga tatgataaag cagaagaatt cggcctgcct ttagcaaaat ctgataagta
    18481 ttatggctta gcccttggtg gactggaaaa aggagtatct ccattgatca tggcgggtgc
    18541 ttacagtgca tttgccaatg atggtcagat gtatacgccg catcttatca cgaaaatcgt
    18601 tgattcgact ggagcggtga tcgtcgataa cacaaacaaa aaaccaaaac aggtcatatc
    18661 aaaggaaaca gccgatgaaa tgaccagcat gcttcttggt acattttcaa atggaacagc
    18721 ggctgctgcg aatcctgcag gttatacgat agcagggaaa acaggaacca cagaaactaa
    18781 tttcgatgcc acaaaagcaa atgaccaatg gatgatcggt tacacgccag acgtagtgat
    18841 ttcgacatgg atgggttacg aagaatccag caagctgcac tatttggaag ggacaagtgg
    18901 aaatgttgtc gggaaagtct tcaaatcagc ggcagaaggc atccttccgt atacttcaaa
    18961 aacaggattt agtgtagcag atgcctatgc cacaggagga caagtggtag ctgcagatca
    19021 agttggaaac acaggaaatt ctaatggaga gtctggaaat tggcaagata acttgaatca
    19081 atatggtgaa caagcaaaag aagggctgaa aaacttcggc gatatggtga aagaaggcgt
    19141 tcagggaatt ggtgaagcgg ctaaagacct ctggcggaaa tatcaaggag agtagcccct
    19201 ttgaaaacga ataaccccat gttacaatag agagtaacta ctaaaagaaa agagggcata
    19261 acatgtcaaa catctacgat agcgcgaatc aaatcgaacg tgagatccgt gaattaccag
    19321 aattcaaagc gttgaaagaa gcgtacgaga aagtacaagc aaacgaagaa gcacacaaac
    19381 tgttcaaaga cttccaagcg atgcaaatcg aattacaaga aaaacaaatg agtggacaag
    19441 agttcagcga cgaagatgca atgaaagcac aagaaatggc tgtgaaaatc caatcagaag
    19501 aagtaatcaa tgacttgatg caaaaagagc aaggcttcag tacaatcatc aatgacttga
    19561 accgtatcat catgacacca gttcgcgacc tttacagcga ataaaactat tttatgaaac
    19621 aacagagggt gtgaaagaga cgttcggcct tgagcgatga caaaattgct tcttattgcc
    19681 gataggctgt ctcttgaaca ccatttacca agaggtcgtg agcctaccgt ttaactctga
    19741 gttacaagga acaatttcaa aaaacagctt ctcatgtttg atgactattg tgacttgtga
    19801 ccgaggagtt ggcaggagaa caccgtttat tcagaaatag gagctacgac aaaagtcttg
    19861 tcatagctcc t
//
LOCUS       DACESX010000046        18957 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.046, whole genome shotgun sequence.
ACCESSION   DACESX010000046 DACESX010000000
VERSION     DACESX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.046"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..636)
                     /locus_tag="IXF88_001366"
     CDS             complement(<1..636)
                     /locus_tag="IXF88_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508105.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIA"
     gene            complement(1097..1411)
                     /locus_tag="IXF88_001367"
     CDS             complement(1097..1411)
                     /locus_tag="IXF88_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1508106.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(1427..3022)
                     /locus_tag="IXF88_001368"
     CDS             complement(1427..3022)
                     /locus_tag="IXF88_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAQ1508107.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(3045..3935)
                     /gene="iolE"
                     /locus_tag="IXF88_001369"
     CDS             complement(3045..3935)
                     /gene="iolE"
                     /locus_tag="IXF88_001369"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAQ1508108.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(3952..4977)
                     /gene="iolG"
                     /locus_tag="IXF88_001370"
     CDS             complement(3952..4977)
                     /gene="iolG"
                     /locus_tag="IXF88_001370"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAQ1508109.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(5001..6008)
                     /locus_tag="IXF88_001371"
     CDS             complement(5001..6008)
                     /locus_tag="IXF88_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1508110.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(6121..7281)
                     /locus_tag="IXF88_001372"
     CDS             complement(6121..7281)
                     /locus_tag="IXF88_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAQ1508111.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(7357..8811)
                     /locus_tag="IXF88_001373"
     CDS             complement(7357..8811)
                     /locus_tag="IXF88_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAQ1508112.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            complement(8875..9699)
                     /gene="iolB"
                     /locus_tag="IXF88_001374"
     CDS             complement(8875..9699)
                     /gene="iolB"
                     /locus_tag="IXF88_001374"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAQ1508113.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(9738..11654)
                     /gene="iolD"
                     /locus_tag="IXF88_001375"
     CDS             complement(9738..11654)
                     /gene="iolD"
                     /locus_tag="IXF88_001375"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAQ1508114.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(11665..12699)
                     /gene="iolC"
                     /locus_tag="IXF88_001376"
     CDS             complement(11665..12699)
                     /gene="iolC"
                     /locus_tag="IXF88_001376"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAQ1508115.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(12856..13872)
                     /locus_tag="IXF88_001377"
     CDS             complement(12856..13872)
                     /locus_tag="IXF88_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1508116.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(13974..14171)
                     /locus_tag="IXF88_001378"
     CDS             complement(13974..14171)
                     /locus_tag="IXF88_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508117.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(14143..15462)
                     /locus_tag="IXF88_001379"
     CDS             complement(14143..15462)
                     /locus_tag="IXF88_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAQ1508118.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(16203..17183)
                     /locus_tag="IXF88_001380"
     CDS             complement(16203..17183)
                     /locus_tag="IXF88_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1508119.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(17196..18074)
                     /locus_tag="IXF88_001381"
     CDS             complement(17196..18074)
                     /locus_tag="IXF88_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1508120.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(18090..18890)
                     /locus_tag="IXF88_001382"
     CDS             complement(18090..18890)
                     /locus_tag="IXF88_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAQ1508121.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
BASE COUNT         5181 a         4431 c         3327 g         6018 t
ORIGIN      
        1 agcaattaga tttcgattca aaagaataat attatcaaaa tacttcttta ccttgcttaa
       61 atcatgatgc acaattagca ctgtttttcc ctgatgtttc aattctttta acgtatccat
      121 aattattcgt tcactaacta agtctatccc tacaaatggt tcatctaaaa atatgaaatc
      181 cgcattttga gcaagacatc ttgctaaaag tacacgttga aattgtcctc cagataattc
      241 tcctatttga tgattagaat attttcccat atcgaccttt tccaaagcct ttgaaactct
      301 ttgccattct gcatttttaa ttctttgaaa aactttcatt ccggcatatg ttcccataga
      361 tacacattct tttactttta ttggaaaagt aaaatcaata tcagcttttt gctctacata
      421 tgcaatcttc accagttttt tattgatatc ttcattatta atggagacct ctccctgatg
      481 agggataata tttaacattg ctttaagcaa tgtagatttt ccagcaccat ttggaccaat
      541 aatacctgtt aaagcacctt cttgtatttt taaagaaatc tcgttcaatg ctaaaaaatc
      601 gttataacaa acattaagct ttttcacttc taacataact ttctccttct aaatgataat
      661 gattatcacg atcatatggt atacaaaaat tatattttag tcaaccctaa attttataat
      721 ttagattttt ttctgttgtt gaagattagg atgtcaaaat tatttgaaac tactcgtata
      781 gtaaatagta agtaattttc aaaacacgaa gaaaaacaaa aagaaaaaag gaaacgccga
      841 agcatttcct ttcagtgatt taatgagaac aacacgattt gacttagagt ctcaaaaagt
      901 cgaataggta cactcattta agtgtctcca ttcgactttt tggggatgag ctggctattg
      961 aggtccgctt accctattga ataaatacaa gattgggtat ttctaataag tccgcccttc
     1021 atattggggt gatcggggcg aacatgccat cttttcatat accacctcct cgacactcaa
     1081 taatcaaaga ttctcgttaa tcttcttcga tgcttgcaat gatgttttca atattcatat
     1141 aacgaaaatc ttcttgtttg atataaacga tctgagcggt acatttttcc tttttgatga
     1201 tttcttttac tttttcctca gaacgtgcga cctgcggcgt taaaagtaaa acgtcatagt
     1261 ctttataatt cagtttttct tcaacgtttc ggatcgttgc ataatctatc tcatacgata
     1321 gatttttggc tctgatcgtt tcaagcattt ttgatacgag cagcgacgca ctgattccgg
     1381 tctcgcaaat aatcaataga tccttatgca taataccctc ctgtttttag cctgcaaagc
     1441 caattggtga aaatgccaca taagttaaaa taacaatgac taccaatgct atagcagtca
     1501 ccttggtgta tttccaaggc tcgatatcga cctttgtatc gaatgagttc aacacaaatt
     1561 ctccatcagg tttcaatttg gaaaccacca tcaaaatgat aatgtccaga acgaataatg
     1621 ccgtcaaaac ataaaggaaa tggatatttt ggaaaagcat ttttgatagt gcatacaaga
     1681 cgatatgcac ggcaatcgtc gtttttgccc caaaagcagt tgcttttttt gaataaaagg
     1741 caaagagaat gatcacgagc agcggcatgt tgtatagtcc attgaattct tgaacgaact
     1801 gatacaatcc agccggtgca aaagaaatga atggcgctac gataatggag ataatcccca
     1861 caacgaccgt tactgtttta ccagcacgag cgatttgctt atcatttgct ttcttattga
     1921 taagcggttt ataaaaatcc agtgaaaaca aggtcgatgt cgcgttcaaa gctcccgcaa
     1981 atgatgataa gatcgcgcca aagataacag cggcgaaaat accaaagatc attttaggca
     2041 atacttcact gactaaaaaa ggatacgcgt tatctggatt tgacattaaa ctatcgccaa
     2101 aaatattgcg agcgacgatc cctggaaaga caaggaataa cgcacctaag attttgaaga
     2161 cgccgactag gaaagcgccc ttttgtgcct cagctaaatt cttgccggac aatgcttttt
     2221 ggacgatcat ttgattggta caccagaaat acagattgtt aaacagcatc cctgtaaata
     2281 gtgtcggcca tggaacaaca gccgagtcga tcgatcccac agagttaagc agccatggcg
     2341 tgtttaccac aacgtgttcg atcccgccaa caaagctgcc gtcaccaagc ttcattaagc
     2401 ctaaaattgg gaccagcaga ccacaagcca gcagaccgac tccataaatg gagtcactat
     2461 tggcacttaa cgacaagccg ccgatcagca gatagaggat gccgacaata ccaattgcca
     2521 tcgctacaat tatgatcgcg acgatcggct ggacgcccag cagctcatca atatggaaga
     2581 tcttattgaa caccagcgat ccagaataaa gaaccacggg taagaaagaa gtcatatagg
     2641 tcacaataaa taagatcgaa ataattcgtt tggtaaccgt atcataacga atctcgatga
     2701 aatctgagat tgtattgatg ccgtatttaa aatattttgg caagaatatc acagccaaag
     2761 ctacgatcgc aatcgtagca gttacttccc aggccattac ttccatccct gcatgataac
     2821 tttgaccatt ttgtccgact atttgttcgg tggacaggtt cgtcatgatg atcgtgccgg
     2881 cgattggcaa agcagttaag ctccgccctc ccatgaagaa cccttctgat ccagaagtat
     2941 cgacacttct ggatctagaa taggcaaata accatacaca gatgacaatc actaggaaac
     3001 tgattataga aaaaagattc atcgtctagc tccttttcaa tagattaaag accggtgacc
     3061 tctcgaatgt agcttctggc tttgactgca tattcaaatg gattggcttt agccgggtcc
     3121 tgttctgctt caactacgat ccagccttca tagtcgctgg catggattgc tgtccagatt
     3181 ggagtgaaat caatcatccc atcaccagga accgtgaaga cgcctttctt cacgccgttt
     3241 aagaagctgt ctttttcctt ttctacttct tcagccgttt cttgacgaat atctttgaaa
     3301 tggatgtgtt tgatacgatc ttgatgacgg ttgtaaaccg cgattggatt ttcaccagaa
     3361 aagaccaaat gtcccgtatc gaacaataat ttaactttgt ctggatcagt catttccatt
     3421 aaacgatcga tttcttccgt cgtttgaacg ccggtaccca tgtgatgatg gtaaacgatc
     3481 tccatatcct tttcgtgggc caaatcccct aatttttcta atccttccgc cagttttttc
     3541 cactcttcat cagtgaaaac tggtttatca tcaaagactg gcgctgtcat aatcccttgg
     3601 atactatgac cctgctcagc tactacgatg actttagcgc ccatcgcatg taagaaatcc
     3661 cgatgtgctt tgaaggcttc ggcagtttct tcaaaaggtt tagttgtcag aaacgcactg
     3721 aaccatgcac tggcaacctt taagccgcga atattcaaat attgttccaa cacctctgga
     3781 tcttttggat atttgttgcc aatctctgtt ccggtgaacc cagcaagggc catttcactg
     3841 atacattgtt caaaggtatt ttcactacca agctcgggca tatcatcatt ggtccacgcg
     3901 attggtgcga tggctaattg aatttgtttg gtcatattct cacactcctg cttatttttt
     3961 tagttgtact aaactcttcg tatcgaatga ttcttgacaa gccaacgcga tcttagttga
     4021 ttgcaatcca tcttctgcat taaccgctgg ttgacgtttt tcttgaatac atgagacaaa
     4081 ttcttgggct tcacgaatga aggcttctct gaatcgttct gggaagtttt gtgaacatgg
     4141 acggatgatc ccttgatcgt tcatgatcgt cacaagattt ttctcaggcg cttgcgctac
     4201 ccgcagcatt cctttggttc ccatgatctc tgtttcgaca tgatagccat gggctgcgtt
     4261 tctgccggca actagtaaag ctattgtttt atcgctcagg ctcatcattg cggctccagt
     4321 ttcaagctca ccagcctgat ccaattctgg atacgccgca tttttcccaa tcgcccacac
     4381 ttgatcgact tcttggccgg taaaccagcg aaccaaatca atatcatgga tcgccatatc
     4441 taagaacaag ccgccgctgt cgttattcat ggcaaatttg acaaagcttt ccaagccttc
     4501 gctcggatcg atcccataac aacgaatcag tgtgatttcg ccgatttcgc catcttctac
     4561 cattttcttc gcatactgat acgactcatc atagcggcgc atgaagccta acatgaagac
     4621 ctgttcagga tgctcttcga tgactttttg ggtcttttta atatcttcaa cttcaaggcc
     4681 taatggtttt tcagtaaaga catgcttgcc attttccatc gccaagcgga cttggtcaca
     4741 gtgaaagcct gatggtgaaa caataaagac tgcatcgatc acggaacttt tgaccatttc
     4801 ttcatagctg gaaaaagtat gcttgacgcc taatttcttc tgtgcgaatg ctaattctgt
     4861 ttcaactaaa ctgcacgcag ccgtaagctc acaacaactg acacctgttg ctaaattttg
     4921 tgcatgtact tttcctaatc ttcctaaacc gacgatgcct actctaatgt tgttcatgaa
     4981 atacctccgt tttctgagaa ttactcattg tatagtgctg gtcgttcatc catttgaatc
     5041 attacttttg tttttgtttc acgagactca tgacaagcca tcatggtgat cgctgctaca
     5101 tacccatccc aagcggtagg accgacaacc gcatcatttt tgatcgaatc gatccacagc
     5161 ttgaattcat aatcataggc atccaaaaag cgggtctgcc aatctggtga aacaggtgtg
     5221 taattttttt cttccgattt aattcgtgta taggctgggt ccgtcattcc tacttctgcc
     5281 gtttcgaaaa ccacatcaca tttaatatcg tagccaaagc ggcaattcac aaagatttcc
     5341 aaatcaatcg ttacaccgga ttttgtttga aagatcagca gctgcggatc atgcaaatcg
     5401 gcatgggagt tgctggtttt tttcggcaag atcatttgaa cagattcata atcttcattt
     5461 agcaaccagc gaagcacatc aatctcatga atcgcggtgt tgctgatcgc catcggtgtt
     5521 gtgtagtttt catctgcgct aggatttcta tgggcacaat gcatcattaa tgcttctccg
     5581 tattgcttcg attcgattgc ttctttcagt tcaatgtagc cgcggtcata gcggcgcatg
     5641 aagccaacct gaaccaattt cttcccataa gcaacttccg catcaacgat ccggcggcag
     5701 cctgctgcat ccatcgctaa tggcttttcg cagaaaacat atttcccatg cttgattgct
     5761 tctaacacat actgctcatg ggtcgggtcc caagaagtca caacaacgac atccacatct
     5821 tcagaagcga tcaatgcttc tccggtttca aagaatttag catcgacacg tttggcaacc
     5881 tctttagcag cttttacatt gatatctgag actgctgtca cataagcccc ttgtgttttc
     5941 tgattgatcc tgtcgatatg ctcgatgccg attgcacctg ttccgataac gcctactttt
     6001 actctcaatt acttatcccc ctgttctgtt gtttaagcac acatagtatg cgcttacaaa
     6061 attattcctg tgtagctttt attcttaaaa ggtagagccc tctctttgct ccgcctttta
     6121 ctacccataa gcttgtacgt ataattcctt aatttgacta tgactaggca cctttttatt
     6181 attttgcgga cttccgctgg tcaacgcatc cgcggccatt ttgtctaagg ctgccataaa
     6241 cgcttgctga tcaataccat aatctcgaat cgttgggatc gacagctgct cacagagctc
     6301 ttggattttt ttaatcagat aatccgcagc ctcttccagc gataaatctt cggctgccgg
     6361 ataaataaat ctggcgatcg tcgctaactc agtctgaata gcatcctttg agaattcaaa
     6421 aaagaccggc aataacatcg catttgatat cccatgagga atgtgaaaaa gtgcccccac
     6481 cggtcgactc attccatgaa tcaatgtgac tgacgcattc gaaaaggcaa tccccgcctc
     6541 cgttgaagct aagctcattt gttctctggc ttcagtgtcg tcgctatttt catatgcctg
     6601 agaaagcgtg ttcaaaattt tcttcatcgc ttccagtgaa aatagttgcg ttacttcatt
     6661 ggctttgact gaaacataag attccaaagc gtgacatagc gcatcgactc ccgttgccgc
     6721 ggttactttt ggtggtacag ttttcgtaaa atcagagtca acgattgaga tctgtggcat
     6781 gaaaacgaga tccttcatca tcattttgac tgctgttttt gtattggtaa taactgccac
     6841 atcggttact tccgaaccag ttccagcagt tgttgacact gcaatcagcg gaatgcccgc
     6901 ttgcagctga tcaaaatttc ttgcatgaat cgcttcgaga cttaattgat tagcagccaa
     6961 aacggctacc accttggctg tatccagaca gctgccgcca ccaaaagcaa tgatatgatc
     7021 acattgatgc ttctgaaaaa gcttcagcgc atcataaaca ttttcttgcg tcggttcggc
     7081 ttgaatgtct gtatactccg ctaattgatc atctgttttt cccaacagct ccttgatctt
     7141 ttgaagatgc ccggtctgtt ccataaccgt atcactaatg attaacggct tgttgcctag
     7201 ttgttctgat tcttccataa gaaattggat tgttccgccg ccaattctta aattggcagg
     7261 cattttcagt tgaatattca cagaaaaaac ctctctctgg cttatagcct ataacagaat
     7321 cctcgaagct ctttaacaga gcttcgaagc aaatctttaa atctcacttt gcgcataacg
     7381 tgctgtaaca actttccgat gggtataaaa ctcaacactg tctttgccat tggcatgcaa
     7441 atcaccaaag aaagagtgct tccagcctga aaaggcaaac caagccatcg gtgcaggcac
     7501 cccaagattg acccccagca tcccagcatc aacattttct ctgaagtagc gaatcgctga
     7561 tgcgttgttg gtatacagac acgaaccatt cgcaaattca gattcattcg tcagctcaac
     7621 ggctgccatc aaatctttta cccgcacgat tgatgcgacc ggggcaaaaa tctcatcctg
     7681 ccagatcttc atatccatcg tgacattatc aaaaatcgtt ggtccaacaa aatagccttt
     7741 ttctggaatg ttttcgcgac catctagtac cagtttcgct ccttcatcga cccctttttg
     7801 gatatagtca aaggtccgct gctgtgcatc ttttcgaatc actggtccta aaaagacccc
     7861 ttcgtccaag ccattgccga tcttgatcgc ttttgccgca gccttaaatt tttcaacaaa
     7921 ctgatccgcg atttcctctt ccaaaacaat gacggaggct gccatgcagc gttcaccagc
     7981 agaaccaaaa gctccgccaa taaggtcctt gactgcccga tcaatattgg catctttcag
     8041 cacaacaaca tgatttttcg ctccagtcaa cgcttgtacc cgttttagat tttttgttcc
     8101 ttcacggtaa acgtattcac cgacattttt tgatcctaca aagctgatgc cttttaccag
     8161 tggatcttgc agcaatgtgt tgacaacatc gtgggcacca tagacaacat taaaaacacc
     8221 gctaggaaag cctgcttttt caaccagctc aacaattttc tccatcaaca atggtgtttt
     8281 ttctgaaggc ttcagcacca ccgtatttcc tgccgccaga gccatcggga agatccagaa
     8341 gggcaccatc attggaaaat taaacggtgt gatccctccg attactccga ttgggtaacg
     8401 ataattcgtg atctcaatat cagtcgccgc tgttgataaa gaatctccca tcattaaatt
     8461 ggtgattccc gctgcgtgct ccacattttc gattcctcgt tgaacttctc ccagtgactc
     8521 ggacaatgat ttcccatttt ccagcgtgat caaacgcgcc aattcacttt tatgatctgt
     8581 taaaagctga tgcagcttga acagataacg tccgcgaaca ggaacaggaa cttttttcca
     8641 agtttgaaac gcagcatcgg cagctttaac tgcttcggct gtctctgctt tgctagacaa
     8701 cggtacattc gccagcgctt cttctgtagc cggattcatt acttcttcaa actctgttga
     8761 gcgactagcg acccaacgac catcaatata atttcttaaa aatcttgtca tttattttcc
     8821 tcctaaaaac ctaactaaac ctaactaaac tttatgtatc taacagctta ataactactt
     8881 gcgctgcaag atccattcgt gtttcttttc attgtggaat ttccaaattc gctcaggtcc
     8941 cgccatcaca tttaaatagt aggaagtata cccgtcaggt accccaactg gatgatagcc
     9001 cttaggaact tcgacgacat tttgatgctc taccgtcatc gtttcatcta gtgaacgatc
     9061 ttccgtgtag acccgctgga aaacaaatcc ctgctctgga tcaagctcat ggtaataagt
     9121 ttcctctaaa aaagactcat gtggcagatt ctcttgatcg tgcttatgag gaggatagct
     9181 ggaaaaatta ccgccctcgg tataaacttc gacgaccagc aggctgctgg cagtatcgac
     9241 attatccggt aaaatgttgt gaacaagtcg tttattctga tagagtccac gttcttcgac
     9301 ggtattatcc tccgcgcgaa tcacacttat cggcaaagct tttttcgctg gtgaatagca
     9361 aagcgccaat cggacattgt ctgtagttgc cacaagttca aacgaatgct ccgccggaag
     9421 ataaatactg tcggttggaa ttttttcaaa aacagattct cgtgttccta aatcttcata
     9481 ccaagtgtct gctgctttaa ccttaatttt tccagtcaaa atgacgatac agacctcctg
     9541 ttgactgccg gtttccttgt aagaagctcc cttttccagc tccaaaatct gaaattcgat
     9601 ataactcaaa gcagaattag actgccgaat attttggatt tgttttactc caggtgccag
     9661 ttctttgttt aatggttgat gcttcaatgt cttttccaat caatccactc cttattttca
     9721 atcacaaaaa gcaatattta atactttctg atagtggcaa gtttcgcttg tttggctttg
     9781 taagcctcct gcatggctgc tttacccgat acctctgaga taccgacgtt ccaccagctg
     9841 ccgtcgtagc cttctgtcat ggttttagga agtaccttta tctcaatcaa cgtcgatcgc
     9901 gtttgcttct tcgcatcctc gaccgctgct tgtaattctg caatcgagct tacacgatac
     9961 gtttttgcac catagccttc agcaactttg gcgtaatcga tgttcatgat ctcgtccttg
    10021 gtatttctga attcggttcc ttggctagct ccgccattat ccatctgtaa attattgata
    10081 cagccgaaac cagaattatc gaaaagtaca atgttgattt ttttatcata ttgcaaacta
    10141 gaaaccagct cgctatgcaa catcaagaaa ctgccatcgc ctactaaggc atagacctct
    10201 tgttctggtc gagccattct tacgcccatc gcaccagcga tttcatagcc catacaagaa
    10261 taaccgtatt ccaaatgata ggtattctcc ttcacagggt tccataagcg ctgcatatcc
    10321 cccggcaatg aaccggctga accgatcacg atcgcatcct catcaacaaa atcatttaaa
    10381 tgaatgaaga cctctgtttg cgtcaaattc gtagccaacg catcagcata ttcattcaag
    10441 atttcttgag aaaaatgatc tttgatttct ggcgtaaaat ctttacgctt aaaatgcgcg
    10501 gcttttaacc gatttcgctc actgatccat tcttccttca gttcatcgat cttctcgcca
    10561 taggcggttt ggtaaccttc gatcattggg gcaagctgct ccaaggtcgc tttcgcatca
    10621 ccaaccactt gaaaggcttc cagcttagcg gcctgttgtc tgcttacgtt aatattcatc
    10681 attttcgctt gttcaaaatt aaaaatactt ttagaagctg aggtaaaatc agtgtagcgt
    10741 gtgccgatcc ctatgatcag atccgcttgc tgcacagctt tattagcagc cgaagttccc
    10801 aaaatcccca ttccgccgag attgttttta aaatcccacg aaacagtcga tttacctgcg
    10861 tgcgtttcaa ctagcggaat ctgacaatct cttgagattt gctcaagaat ctcaccagcc
    10921 tccgaatatt tgcagccgcc tcctacgatg atgacaggtc gtttactggc ctttattcgt
    10981 tgactcgctt cagcaagctc acgagcggtc ggaatcctgc gatcaatata atgtactctt
    11041 tttttgaaaa attcctccgg ataggcatag gcttctgcct ccacgtcctg cggcaaacaa
    11101 atcgttgccg gacccatcgt tgccgggttc gtcatgactt caaaggcccg caataacgcg
    11161 gacatcaact gctctggtcg attgatgcgg tcccagtagc gagaaaccgc ttgaaatgca
    11221 tcatttgtcg aaaaggctac actgcttgca tgttccagct gctgcaagac cggatcaggc
    11281 tgacgtgtag caaacgtatc cgccggtaaa aaaagtacgg ggatattatt tgcgaatgcc
    11341 gtacccgctg ctgttaacag attggccgcg cccggtccaa cagatgttga tacggcaaaa
    11401 attttcttgc gtaaattctg tctggcaaag gcagtagccg catgggccat tccctgctca
    11461 ttttttccgc tataactttt caaatgtccg ggctctaatt ccagcgcctg accgatcccc
    11521 aaaacatttc cgtgaccata gacatgaaag ataccttcaa cataaggcgt ttctttgccg
    11581 tcgatgctga tgtattgttg attcaaaaat tttagtaatg cttgtgctgt tgttaaacaa
    11641 accgtctttt ccattttttt caccttaaat ccgagcgatc atttcgccgt atatttcttt
    11701 ctccaatgta ataaaatctt ttacctcctg agtcgtcggt aaagaatctg aacagttatt
    11761 gcttttgacc atcattgatg cttccgctga gccaaactct aaacaatcga tgatctccca
    11821 gccttcgaat aaaccgtata agaaacccga gccgtaacca tctccgccgc caaagccttt
    11881 gcgtgcttcc accggaaacg gcttgattga aaactgctgc tgatcggccg tataagcagt
    11941 cgatcctttc attccatgtt tgattacgac aatctcagca ccacaactgt gccaaaaagc
    12001 agcgctttca ttatctgaca tgttaggctg gatcaacttc tcagttagat cgaactcttc
    12061 tcttgaaccc atgataatgt ctgattcctt agcaatcatc gaatagtaaa ttgaaatatc
    12121 atcgttgttc ttccagttat aagcacggta atcaatatca aaaataattt tgacattatt
    12181 ccttttcgcc agcatcactg ccttgattac ggcttctctg gacgggctct ctgctagtgc
    12241 agttcctgag attaatagtg cttttgaccg tttcaagtag tcctcatcaa tatcctcaac
    12301 gctaagctgc aggtctgcgg cttgatttcg atacatcaaa atattacttt cctttgatga
    12361 gagcatttcg gtaaaagtca gccccagctt ttcaccatgc tgcgctttgc tgatctgaga
    12421 cgtatcgatg ccttgttcat tgaaataatc gatcacatat tcgccaaact gatcgtccga
    12481 gactgttccg ataaaaccag ctttcatgcc atgtttagta acacccacag caatattggc
    12541 aggagatccg cccacaaatt tttcaaacat atggaccttt ttcaatggtt taaactcttc
    12601 tttgacttga tcattgtagg caggattaaa atcaatagcg actcttccta aaagcagcaa
    12661 gtcaaattct tttgattggt caaactttat ataactcaat atatccacct ctttatccgt
    12721 tcttctattt ctgaggcaag tatagcacgc caaaacaaag cgtgcaatat ttattttaga
    12781 acgcaatatt gcgtgctgtt tgaaaatgac ggcaaaaaaa acgtgaatcg ccttacatca
    12841 cgattcacgc ctagattaaa tattgtattt tgtcttaata attatatcag taaatagcgc
    12901 atcttttgta accgttttat tattttgcag ctgattcgct aacatcagta atgaacgata
    12961 gccttgaaca aatccatctt gatcgattag aaaatcaacc tgatcgtttt tcagcatctc
    13021 gatattgttc ttagtcaaat cataaatgat tacaaagggc cgcttgcttt catcgatttt
    13081 ttccaacgct cgggcaattc ccgcttgtcc gccagaaaag acgactacgc catctaagtt
    13141 tgaataattc tccaaggtgt tgcttaaaat cttttcaacc tcagcactcg aatcaaagct
    13201 gctctgaaca cccgtcagct ccagttcagg aaacgactct ttcaattctt caacaaagcc
    13261 tgccacccgt aagctgttga cgctatttcc aaagtagccg gtaatcgcta gaaccttgcc
    13321 ctttcctttt gtcagcatac ctaacaatcc cgcagccgtt tttccgcttt tgaagttgtc
    13381 catcccaaca aaacactgtc gattcgtacc aagaatatcc gaattaaagg taatgatgag
    13441 gatgccttga tcggtcaatt gattaatttt ttctctgata ccatcactat ctactggcat
    13501 gatcgcgatc ccttttactc caagctccac caatcgatcc aacgcctgca gctgggccgc
    13561 ttcatcgacc ccgtctacct cttctaaaaa acaatcaatg tttcgctttt ttaaatcccc
    13621 gataatagat tccagacctg atcggatcgg aatcataaaa gaagatttcg ccaattgagt
    13681 gacaatcccc agcttgagcg ttgtctcttt tatgaccttc tttttctttg gaacatagtt
    13741 catttccttg gcgattgctc tgattctttc tgcaacctca ggattgatcc ttcctcgatc
    13801 gttcaatgcc cgatccactg tcccccttga cactcctgct ttctcagcta tttctttaat
    13861 tgtacccgcc atcttgcctc tccttttttc ctattgtgtt tattttatca caaaacttaa
    13921 gaaaaccacc ggagaatgaa ttctccggtg gactttttgt atgattaacc ttcttattga
    13981 acatcactga tcttaaactc tattccaagg gcgtttctat aatattctgg atacatctcc
    14041 ttcccacatt tttcacaggt aaattgggga ggattgttcg gatcatttgc aatatctgca
    14101 tcatctaaaa attttactac atccattggg attgattctt tttcaccaca acttagacat
    14161 ataaatttca cgttggcgtt cccaatccca gcgaattgct ttaaaagttt tttctttttc
    14221 ttcttcatac tgttgcctga attctttttg gtcaattttt cgattctcct ctcgcataaa
    14281 ccgctttgca tggttatctt tcgcatattt aataactaac cgtccatttt tgcgcacact
    14341 catcccaacg aattcatagt actcaccgca acaagaacat tctaagggat tttcaccaaa
    14401 ttcttcggtt attctttctt cccaggattt tggtcttaag gcctcattca gattgataaa
    14461 cagctttttt actcgtttct gatactcctt catgaccttt ttcattaatg ttttgattct
    14521 acgactatag attccatagc gacgaatcgt tttgaaattc ttattttgaa tatggcgaat
    14581 cagtgcagct atgaattcgt ctatactcat ggtcatcgtc tctttagtat tcgttcgttt
    14641 gtccatgtag ttgaacatga cagtgtcgcc atcatacatt aagattcggc ttaatgcgat
    14701 cgggccacgc ttcatataac gactgatgta ttggagtagt gactttagat ttgtcctgct
    14761 ccttttagga gcatttacgt aaaacccttc tccattgttc ttgtacgcag cttgtaatct
    14821 tgattgaact ctttgcttgt cccactcacc taacgttcgt ctaattagtt tcaatacagc
    14881 atttttccag tattttctta acatagaaaa aggtaaataa tcgtattctt cccaacttcc
    14941 atctcttgtt aagccaccca tagtaacaac catatgaaca tgcggattaa attctagttt
    15001 tgatccaaag gtgtggattg tagctactat acctgccttc acctttcttt tctcaaaata
    15061 ggcaagaata atttttgcgg cttcatccat tagtcctttc agtaaggaat ctcgatagtt
    15121 ttccatcata aagatttccc gtaacccctc atcgatcgta aagattacgt gccgatgtgt
    15181 cactttaaac atatcattcg ccacaaccgc tgaccacctt tggctttcac ctgttgcaca
    15241 cgttggacag aactttccac gacatttcaa gggaacaaac tttgtttgat gacatccctc
    15301 gcagacaaac agtcggtatc ctttttgaat atctccacaa tatctaaact tgtttacttc
    15361 ccgtttgacg actagccgaa tctttcgccc gtatttctca acaaagttat cccaattatg
    15421 gtgttcgtca aaaaagattg cttcgagaat attcgctttc atagtaatat tttacttgtt
    15481 caaaaacaaa atgaaaaggg ctaaaagact ggtagtgcag cctttttagc ccttaacttt
    15541 ttatactttc tttactgtct aaaaaatatg cggcgcacag aacgcgccgc atatcggact
    15601 aagttttatt caaatttgtc ttcgccaaca caatttgacg aagagccttt tctttcagga
    15661 agacttcggt aatttttagg agttctacct attcttttaa ccgtttattc tctttttcta
    15721 agcgtgtaat ttcatttctg cgaatggatt tcatgacttc ctctaccgag aaaagtacgt
    15781 tatccatatt tttcaacttc ttgaactcat caatacacgc tattagagcg agcttctagc
    15841 tatagactaa cttctttaac agaaaaccct tcttccgtta ccagttttac agcggtaatt
    15901 ttaaatattt catcaaatga acgtcttggc ataatccaga tcttcgcttt ctcttaattt
    15961 ggtgtcaatt ttagtataac ccgtccaact tattttttaa ctaagtgccc ggtttttctt
    16021 gacaactcca cccaaaagtt agattttttg gtctaagttt tagggtcgct tcattactag
    16081 aattcaacaa gagatgtttt ttattttatt taaattatgg ggtcagtttc catttgatta
    16141 caaacttctt tgttttcttt ttttaaacgc aataataaag gttttaattt cagccttttt
    16201 aattagaaaa tcaattttcc caaattattt tctttagctt tcttaacgat ttctgtcgca
    16261 actaccacat ctaatacagc cgaaccaact gttttgaaga tcgtgatttg ttctccactt
    16321 gtccgaccga tcaattcttc attgatcagt tggcctagtt ccccatgata gctgtctctt
    16381 tgaatatacc catcttgcag cggtgaaatg aagtccccag cttcttttag aacaccgtcc
    16441 atggtatcaa aaataacaac atctgcggct ttgattattt ctcgtggaat ttcacacatt
    16501 tccggtgtgt aagcaccgac tccattgatg tgcgcacctt ttttgaccca ctcagcagag
    16561 aatgtcgcac gttttgaagt ggtcacactt gtaatgatgt ctgctccttc aacgcattct
    16621 tgactggttt ttgttgggcg catcgttaca gaaaattgtt gcatcatttt ttcagcaaat
    16681 tggctggcac gctcaaaatc aatatcaaaa atccgaactt cttctaattt acgaacggtc
    16741 aacattgctt ctaattgtga ctgtgcttgt ccgccggttc caatcagcgc gccaatttta
    16801 gcatcttctc ttgcaagaag ttctgttgct gctccttgaa cagcgcctgt tcttaattgt
    16861 gtcaaataag taccatccaa acaagcggat accatacccg tttctggatc caatacgatc
    16921 atcgttgctg gtacacttgg taagtttttc ttgatgtttt caggatacac ggaaacgatt
    16981 ttgacaccta gcgcgccttc gccaccttct acataagcag gcatatataa gctttgtccg
    17041 ttgctttttg gcacatcgat attggtgcga agaggcacac ttgcctttcc tgctgaatac
    17101 aatgacagtg cttttttgtc cgcatcgatg gcttctctca ttgaaaaact tttgataata
    17161 tcttcttttt taagaaataa catttgaatc gctccttaat tagctgctgt aacttttttt
    17221 aattgactgc ccaacatcgt atatgcactg tatagtaaga ccaaaatcac gatccatttc
    17281 aggatatagg tatctaaaga tttgactagg aatacagcga ccaatacccc caatacaccg
    17341 aaggttgaag taaaaagtgt gatttttctg ctgtattcat taaatttaat gaattgaaca
    17401 ctgccgatcg gtactgagaa ggtacaagcc cccatcataa taggaaaggc tgcaactgga
    17461 ttcaagccta gcaagtagac agttaccatc gttaacgcat aagaaccaat gccgatattg
    17521 ttcaatgcac catagacaaa gagtaaaaat cctgccaaga ttaattttcc gccatacaat
    17581 tcactggctg ttccgtttga tggaatccag ttcatttgtc ctgcaaaaat caacaatgca
    17641 gcaatgacca atcctatgcc gacaaacagt ttgatcgttt tctctggtaa cttcacgaca
    17701 aatcttggtc ctaaatatgc accgataatt tggcagataa tacaaacagc tagtgtcttg
    17761 atccctacgt tgatggcagt gatgtaagac aatgccatga cggctaccgg tactacacat
    17821 tgggtattca acgttccggg tagttttttc atgctgaccc atttcaactt tggataaagg
    17881 acggttccga tagcaaagtc ggaaacgcca aatgttgaaa gaaagaagat gataaatgac
    17941 gtgaagggca acagacgcat atcccctggt tcttctttga aattttgttt gtttttgaaa
    18001 aggtctctta gaaagtaaat aataaaatat agattgattg cgacaattag tgtcagcaaa
    18061 atattccgaa tcataaaaaa ttcctcctac tagtaaatct ttctgattcc ctcaaagccc
    18121 tcaccatatt ttgctttaag ttcttcagga gattctgcaa tcacttttag tgtatatgca
    18181 tcatgtttga catattcttt tgctagattg accatggcgt ggttttttgt ccaagtgaca
    18241 tcggttgatt gcagttcttc gttaatggta ccaaacacga cttcttgctc atcggcgacg
    18301 atgttgatcc atcttgctcc aaagtcagca agtttgtctg tttcaaaacc atgaatataa
    18361 tacttttcta agggaagatc atattcatgg gtagaactga ataaaatcag tacaaactta
    18421 tcaactcttt tttcagcttg ctgcaatgcc tttagtagtg aatccgtgaa attctcatgc
    18481 caaacttgaa tgagcaaact ttcttttgcg tttttcacca aatgctctac tttatccaga
    18541 acatactcga taccatcaac tttccacagc atttcttctt catctttttc tttgatcatc
    18601 cctagattct tggtaagaaa agaaagatca tttttaacac ttttttctaa tttctctaaa
    18661 aactcatttg ccgagatggc atgataaagt ttaggttctg atttattaac gacaatcaaa
    18721 ttctttttta atagtttttc caaaatatta tatactttcg agcgtggcac acctgagatt
    18781 ttacttactt catatcctgt aagattacct ttttctaata gtgtggtata cactcttgtc
    18841 tccatctctg aaaaatcata ttttttcatg atttgaataa tcgtttccat tgattaatcc
    18901 tcactttatt tttagtagct actacggtaa caactataaa ttctttttga ttatctg
//
LOCUS       DACESX010000047        17707 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.047, whole genome shotgun sequence.
ACCESSION   DACESX010000047 DACESX010000000
VERSION     DACESX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.047"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..808
                     /locus_tag="IXF88_001383"
     CDS             <1..808
                     /locus_tag="IXF88_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1508122.1"
                     /translation="RFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNRRL
                     PRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFFDT
                     FVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEIVT
                     AVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFLLW
                     QIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            805..1605
                     /locus_tag="IXF88_001384"
     CDS             805..1605
                     /locus_tag="IXF88_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1508123.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1766..3034
                     /gene="rseP"
                     /locus_tag="IXF88_001385"
     CDS             1766..3034
                     /gene="rseP"
                     /locus_tag="IXF88_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1508124.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3102..4811
                     /locus_tag="IXF88_001386"
     CDS             3102..4811
                     /locus_tag="IXF88_001386"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1508125.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETNKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5023..9375
                     /locus_tag="IXF88_001387"
     CDS             5023..9375
                     /locus_tag="IXF88_001387"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1508126.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9519..9992
                     /gene="rimP"
                     /locus_tag="IXF88_001388"
     CDS             9519..9992
                     /gene="rimP"
                     /locus_tag="IXF88_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1508127.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10016..11191
                     /gene="nusA"
                     /locus_tag="IXF88_001389"
     CDS             10016..11191
                     /gene="nusA"
                     /locus_tag="IXF88_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1508128.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11213..11506
                     /locus_tag="IXF88_001390"
     CDS             11213..11506
                     /locus_tag="IXF88_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1508129.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11503..11814
                     /locus_tag="IXF88_001391"
     CDS             11503..11814
                     /locus_tag="IXF88_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1508130.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11827..14133
                     /gene="infB"
                     /locus_tag="IXF88_001392"
     CDS             11827..14133
                     /gene="infB"
                     /locus_tag="IXF88_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1508131.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14160..14507
                     /gene="rbfA"
                     /locus_tag="IXF88_001393"
     CDS             14160..14507
                     /gene="rbfA"
                     /locus_tag="IXF88_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1508132.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14615..14836)
                     /locus_tag="IXF88_001394"
     CDS             complement(14615..14836)
                     /locus_tag="IXF88_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1508133.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15284..17647
                     /locus_tag="IXF88_001395"
     CDS             15284..17647
                     /locus_tag="IXF88_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ1508134.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6035 a         3147 c         3900 g         4625 t
ORIGIN      
        1 acgttttttt ccgcaaaaaa acaaatatat tcaagaaaaa agcgaatttc aatttaatac
       61 cgatggacca attcctcagc acattgccat catcatggac ggcaatggtc gttgggcgca
      121 aaaccgtcgt ttgccacgag ttgctggcca taaagaaggc atggaaacag taaagaaagt
      181 cacaaaaaaa gcatcaaggc ttggagtgaa agtgttgacg ctttatgcct tttcgactga
      241 aaactggaag cgaccaaaag atgaagtaag ttttctcatg caactacctg tcgacttctt
      301 tgatacgttc gtaccagaat tgattaaaga aaatgtcaaa gttcatgtca tgggttatga
      361 aaacgtattg ccagaacata cgcaagatgc tgtaagacgt gcaattgagc aaacaaaaaa
      421 caatactgga atggtattga attttgcttt gaattatgga agtagagcag agatcgtgac
      481 tgctgtccaa gaaattgccg aagaagtagc aaaaggagaa atacacgcag aagaaatcga
      541 tgatgaattg attgctaagc atttaatgac aggtttcttg cctaaagagt tacaagatcc
      601 tgagctgatg atccgtacaa gtggagaaga gcgaattagt aattttctgt tatggcagat
      661 cgcatatagt gagctgtatt ttacaaaagc gctttggcct gattttgatg gtgcgcatct
      721 tgaagaagca atcgcctctt atcaaaatcg agaccgtcga ttcggcggtg taaaaaaaac
      781 agaagatgag aaaggggatc agtcatgaaa caacgtgtga tcacagcagc tgtggcgcta
      841 gtcttgttta ttccgattat atggatcgga ggcatagcgg ttgagcttac agcagcaatc
      901 ttagcggtag taggagttta cgagctgttc cggatgaaag gattgacact cttaagtttt
      961 gaaggagttt tatccgccgt tggtgcagtg ttacttgttt tgccaaaaga aagatggttt
     1021 ttctttcttc cagagaatgt aagtacattt atgttatttt atcttatagt tatgattctt
     1081 ttaggaacat cagttatttc taaaaatacg tatacgattg atgaagcagg ttttcctgtg
     1141 cttgtcagct tgtatgtggg aataggtttc cagaattttg tcaatgcacg aacagaaagc
     1201 ttggctgttt tgatatttgg tctatttatt gtttgggcaa ctgatatcgg agcatatatg
     1261 gttggtcgac gttatggtca gcataagtta agtcctgata tttcgcccaa caaaacgatt
     1321 gaaggtgcgc tcggtggtat cggtagtgct gttgtagtcg catttcttta tttattgcta
     1381 tacccagcaa aagaactatt caatcatggt atgtttgtca tgcttctgat gacgattatt
     1441 ttttcagcag tcggacaatt tggtgatttg gtcgaatctg caatcaaacg tcattatgat
     1501 gtgaaagact cgggaaatat cttaccaggc catggtggaa tcttagaccg tttcgacagt
     1561 ttgctgtttg tatttcctat catgcattta tttggcatta tttagtaagt atacctgcgt
     1621 ttctttgtga atttcataaa agacgcaggt ttttattttt cattatattg atactattgg
     1681 tgtctttggt atactatggt aaaattatag tgttttatcg ttgttttata aaaaacacaa
     1741 aaaacaagta aaggagaaaa aagctatgaa aacgattctg acatttatca tcgtttttgg
     1801 tatattagtg attgttcatg agtttggtca tttcttcttt gcgaaacgat caggaatcct
     1861 cgtccgcgaa tttgctatcg gtatgggtcc gaaaatttat gggcatcaag caaaagacgg
     1921 gacaacatat acgcttcgcc tattacctat tggcggttat gttcgaatgg caggcaatgg
     1981 ggatgatgaa acagaaatgg caccaggaat gcctctttct cttttactaa attctgacgg
     2041 catcgtagag aagataaatt taagtaagaa aatccaattg acaaatgcta tcccgatgga
     2101 attgagccgt tatgatttag aagatgagct gactatcact ggttatgtga atggagacga
     2161 aacagaagta gtaacctacc ctgttgatca cgatgcgacg attattgaaa acgacggaac
     2221 agaaatcaga attgcgccaa aagacgtaca gttccaatcg gctaaattgt ggcagcgtat
     2281 gctgaccaac tttgctggac ccatgaataa ctttattttg gcaatcgtgt tattcatcat
     2341 tctggcattt atgcaaggtg gtgttcaagt gacgaacact aatcgcgtag gagagatcat
     2401 gcctaatgga gctgcggcag aagcaggctt aaaagaaaat gatgaggtag tcagtgtgga
     2461 cggaaaagaa atccattcat ggaatgatct gacaacagtc attacgaaaa atccaggtaa
     2521 aacattagac tttaagatcg aacgagaagg tcaggtgcaa tctgtcgacg tgacaccgaa
     2581 atctgtagag tcaaatggcg aaaaagttgg ccaacttggt ataaaagccc caatgaatac
     2641 aggttttatg gataagatca ttggagggac gcgtcaagct ttcagcggtt cattggaaat
     2701 tttcaaagcc ttaggatcac tatttacagg tttcagttta gataaattag gcggacctgt
     2761 catgatgtac caattgtctt cagaagcagc aaatcaaggt gtaacaactg ttattagttt
     2821 gatggcgctt ctttcaatga atctcggaat cgtcaatctg cttccgattc ctgccttaga
     2881 tggcgggaaa ttagtcttga atatttttga agggattcgc gggaaaccat taagtcaaga
     2941 aaaagaagga atattgacat tagctggctt tggattttta atgttgttga tggtgctagt
     3001 tacatggaac gatattcaac gattcttttt ctagaaaaag ttgacaaata gggtataata
     3061 acaaacgata actattttga ggaaataaag gagtatatag aatgagacaa tcaaaaatgt
     3121 tgattccaac attacgcgaa gttccaaatg atgcagaagt attaagccat caaatcttgc
     3181 taagagctgg atatatccgc caagtatcag cgggtattta ttcatatctg ccattagcaa
     3241 atcgtgtatt ggaaaaactg aaacgcatta tgcgggaaga atttgaaaaa atcggagcgg
     3301 tagaaatgct gatgcctgcc atcttgccag cagaactttg ggaagaatcc gggcgttacg
     3361 aaacatatgg accaaacttg tatcgtttca aagaccgcaa tgatcgaaaa atgatccttg
     3421 gaccaactca cgaagaaact tttactgatt tgatgcgtaa tgaaatcaat tcatataaga
     3481 aattaccttt aaatctttat caaatccaac ctaaatatcg tgacgaaaaa cgacctcgct
     3541 tcggtttgct tcgcggaaga gagtttatca tgaaagatgc atattctttc catgcaacgg
     3601 aagaaagttt ggacgagaca aataaagatt acgaaaaagc ctataccgaa gtattcaagc
     3661 gttgtggctt agaatttcgc tcgatcatcg gagacggcgg cgcaatgggc gggaaagact
     3721 caaaggaatt catggcgatc tcagaaatcg gggaggatac gatctgttat tcgactgaaa
     3781 gtgattatgc tgcaaacttg gagatggcta caagctacta tgtatcaaaa aaatcgcatg
     3841 aaacacagct tgaattggag aaagtagcta cacctaacgt aaaatcaatt gacgaagtag
     3901 cagatttctt ttctgttgaa cctcaaaaaa tcatcaagtc tgtattgttc attgcggatg
     3961 aacaacctgt ccttgttttg gtacgtggag accatgaagt caatgacgta aaactgaaaa
     4021 actttttatc tgctgacttt ttagaagaag caacagaaga agaagcacaa aaatatctag
     4081 gtgctgagtt tggttctgtc ggaccagtcg gtgtttcaga tgacgtaaaa gtatatgcag
     4141 accgttatgt ccaagattta gcgaatgcca ttactggagc gaacgaaaca ggattccact
     4201 ataaaaatgt gaatccaaaa cgcgatttca ctgtgctttc ttatgaagat cttcgttttg
     4261 tacaagaagg agatccttca ccagacaaca acggtgttct tgcctttact cgaggaatcg
     4321 aaatcggaca tatattcaaa atcggcactc gttacagtga atctatggga gcaactgtac
     4381 tagacgaaaa tggacgagaa aaatttgtga ttatgggctg ttatggtatc ggtgtaagtc
     4441 gtttattatc agcaatcgta gaacaacatt cagatgaaca aggaatcaat tggccaaaag
     4501 gaattgcgcc atttgatctg catatcgtcc aaatgaatct aaaagacgaa taccaaacaa
     4561 gcctgacgaa tgaactagaa gaagcgatga caaaagctgg ctatgaagta ctggttgatg
     4621 acagaaatga acgcgcagga gtgaaattcg cggattctga tttgattggc tgtccaatca
     4681 gaatcactgt cggtaaaaaa gcagtggatg gaatcgtaga agtgaaaatc aagaaaactg
     4741 gcgaaatggt tgaagtacgt aaagaagaat tggcgaatac tttaccaatc ctcttgaacc
     4801 aaggcgaata ataagcgtta aaacacagaa aataaaaata gctgaatcag ctgtttttga
     4861 caatctgatg attcgctttt aaaattggaa ctgcgatttg aatcgcagtt ccaatttttt
     4921 aatgtaaaac tcagaaaaaa cgagtatact aaaaggtact gaaaaagaag aggataagat
     4981 gcgatcaatt gcattgtttc tcatcagaaa gggcgtatat ctttgtcaga aaaacgagat
     5041 ttatttgaaa aactgttgga acaaatccaa ctagaggaag cagaaaaaaa tcatccactg
     5101 cttactgctg gtgaaatgga tagtgtagtg gtccatcgta aatcgcgttt atgggaattt
     5161 acgctacatt tttccaaaat tttgcctatt atgctatatc ggtcattgac tcagcatctg
     5221 actatcgctt ttaaagatat tgcacaagtc aaactgaata tcttagcaga tgaccagaca
     5281 ttcgatgaac agctcttgca agattactgg gcccaagctt tggaaaatca gcaatgcgat
     5341 acaccactag cacagcaagt attgaaaacg caggtacctg tgataaaaga tcgtaaagtc
     5401 atcttgccaa tcgacagtac aggagctatt tcttatttga agcaacaata cttgccatta
     5461 gtagaagagt tgttcgtcag ttacggattc ccaaaatttc gtatcgaacc agaagtggat
     5521 gagcagcaag cggaacgtgt attgaaactt tttgaagaac gcaaacaaga acaggcagaa
     5581 gcctttatga agcaagcagc tgaaagtcta gttgtccacg aacaaaagaa aaaggaaaga
     5641 aaagagcaat cgccagctct tgaaggaccc atccagctag gacgaaatat tccagccgat
     5701 gaaccaacga caccaatgat cagtatcgtt gaagaagaac gaagagtcac acttgagggg
     5761 tacgtattcg acaaagaagt cagagaacta cgttcaaaac gcaagatctt gactttgaaa
     5821 atcacagatt atacttcatc atttatcgtc aaaaaattct cgaacggcga aaaagatgaa
     5881 caagtatttg atgcgatcag tgtcggtagc tggttaaagg tacgaggaag tattcaagaa
     5941 gatacgtttg tccgtgattt ggtcatgaat gcgcaagata tcatagaagt caaacataca
     6001 cctcgcaaag attatgctcc tgaaggggaa aaacgagtag agcttcatgt tcatagcaat
     6061 atgagtacga tggatgcaac aaacagcatc tctgatcttg tggcacaagc aggaaaatgg
     6121 ggacaccgag ctatcgccat tacagaccat ggtggtgcgc aagcctttcc ggaagcacat
     6181 agtgctggaa aaaaagcagg tgtgaaaatc ttatacggcg tggaagcgaa tgtcgtagat
     6241 gatggtgtcc caattgctta taatgatgca catgaagcat tgagtgaagc cacatacgtc
     6301 gtatttgacg tggaaacgac aggattgtct gcagtatatg acacgatcat cgagctagcc
     6361 gcagtcaaaa tgtataaagg aaatgtgatc gaaagctttg atgaatttat cgatccagga
     6421 catccattgt cacgaacaac ggtagatctg actggtatca cggatgaaat ggttcgaggg
     6481 tcaaagtcgg aagaagaagt attgcggatg ttccttgaat tttcaaaaga tacaattttg
     6541 gtagcccaca acgcggcttt tgatatgggg ttcttgaata ctagctacgc aaggtatggt
     6601 attcctgaag cggccaatcc agtcattgat acgttggaat tagctagata tctgtatcct
     6661 caattcaaac gtttcggatt aggtgtgtta tctaaaaagt ttggtgttag cttggaacag
     6721 catcaccgag caatctatga tgctgaagca actggtcact tagcatggat ctttgtgaaa
     6781 gaggcaatgg acaatcataa catgctttat catgatcagc taaatgagca tatcggagaa
     6841 ggagattctt acaaaagggc tcgacctttc catgtaacga ttttagcaaa aaatcaggca
     6901 ggactgaaag atttgttcaa actgatttca atgtcgaatg tcgaatactt cgaacgcgtc
     6961 ccacgaatac cacgctccca attgaaaaag atgcgtgaaa atctattgat cggctcagct
     7021 tgtgataagg gagaaatatt cgaagccatg atgcaaaaag gggtagaaga ggcaagaaac
     7081 cgagcaaaat tctatgatta tattgaagtt atgccaaaag cggtctatgc gcctttgatc
     7141 gagcaagaat tggtaaaaaa tgaacatgat ttggaagaaa ttatccaaaa tttggtagaa
     7201 atcggaaaaa gtttggataa aatcgttgtt gcaacaggta atgtacatta tctgaacgaa
     7261 gaagatgcca tttaccgaaa aattttgatc aattctatgg gcggagcgaa tccgttgaat
     7321 cgtcatagtt tgccggatgt ccatttccga acaaccgatg agatgctaac tgcatttcac
     7381 tttttagggg aagaaacagc gaaagagatc gtagtagaaa atacgaataa aatcgcagat
     7441 atctgcgaag aagtgattcc agtcaaagat gaactttata caccgaagat tccaggttca
     7501 gaagatgaaa tatcagaatt gagttacaca aaagcaaaac aaatgtacgg agatccgtta
     7561 cctgagatca ttcaaaaacg attagaaaaa gagttgaatt ctattaatgg taatggcttc
     7621 tcggttattt atctgatcgc ccaaaaacta gtgcataaaa gtaatgaaga tggctatcta
     7681 gtaggttcac gtggctctgt tggctctagc tttgttgcga cgatgacagg aatcactgaa
     7741 gttaatccat tagcccctca ttattattgt ccagagtgcc aatattccga gttttttgaa
     7801 gatggtacgt atggttctgg atttgacatg ccagaaaaac aatgtccaaa atgtggtgca
     7861 cgcttaaaca aagacggtca tgatattcca tttgagacat ttttaggatt ccatggagac
     7921 aaagtgcccg atattgattt gaacttctca ggtgattatc aagcagaagc ccataactat
     7981 acgaaagtat tgtttggcga agactatgtt taccgtgccg gaacgattgg tacagtcgcc
     8041 gacaaaacag catatggcta tgtaaaagga tacgaaaggg acaataatct gcagtttcga
     8101 agtgcagaag tcgacagatt agcaaaaggc gctactggag tcaaacggac aaccggccag
     8161 catccagggg ggatcatcgt tattcccgat tatatggatg tctatgattt tacgccgatc
     8221 caatatccag cagacgatca aaattcagaa tggaaaacga ctcattttga tttccactcg
     8281 attcacgata atgtattgaa attagatatt ctagggcatg atgacccaac tgttattcgg
     8341 atgcttcaag atttatcagg gatcgaccca caaacgatcc ctacggatga cccggaagtg
     8401 atgcggatat ttgccggtcc tgaagtactt ggagtaagtc aggaacaaat ttattccaaa
     8461 acagggacat taggtattcc agaattcggc actcgttttg ttcgagggat gttagaagaa
     8521 acgcatccta caacttttgc agaactactg caaatttccg gactatctca tggtacagac
     8581 gtatggttag gaaatgcgga agaattgatt cgacgaggag atgcgacact ggcggaagta
     8641 atcggctgtc gtgatgatat tatggtttat ttgatccatg ctggattaga tagcgggatg
     8701 gcatttaaaa tcatggaaac tgttcgtaaa ggacaatgga acaagatccc tgatgaattg
     8761 cgagaaacct atctatcggc gatgaaagaa aataatgtgc ccgactggta tatcgattct
     8821 tgttcaaaaa tcaaatatat gtttccgaaa gcccatgctg cagcttacgt attgatggct
     8881 cttcgtgtcg cttatttcaa ggtttatttc cctattttgt attactgtgc atacttttct
     8941 gtacgtgcag acgactttga tctagtctcc atgtgcaaag gaaaagatgc tgtgaaacaa
     9001 gcaatgaaag aaatcacgga caagggatta gatgcttccg taaaagaaaa gaatcaactg
     9061 acagtgctgg agttagccaa tgaaatgctg gaacgaggat tcaaatttgg aatgattgat
     9121 ttgtataaat cggatgccgt taactttgtt attgaaggtg atacgttgat cgcaccattt
     9181 agagctgtcc caagtttggg aaccaacgtg gcgaaacaaa tcgttgaagc aagaaaagac
     9241 ggtccatttt tatccaaaga agatcttgcc acccgcggta aagtttcaaa aacattgatc
     9301 gaatatatga acgacaatgg cgtattgaaa gatctaccag atgaaaatca attatcgtta
     9361 ttcgatatgc tataagcatc tgttttcctg tctgcttgta attttaagca gagtatggta
     9421 tagttcttat aggtaatgat actaactgtg agtgagcgga tttttcgctc actcttttta
     9481 atggaattac gcacttaatt tttaaggagg cgaaactttt gagtagcgtc gttgaaacag
     9541 tcacagaaat ggtgacaccg attttagatg aacaaaattt tgaacttgtc gaagtcgaat
     9601 ttgtaaaaga aggaaaaagc tggttcttac gagtatttat tgacaaagaa ggcggtatcg
     9661 atatcgaaga atgcgccttt gtcagtgaga aactgagtga aaaacttgat gcagcagatc
     9721 cagatccgat tcctcaagct tatttcctgg aggtatcctc cccgggagca gagcggccat
     9781 tgaaaaaaga aagcgactat gaaaaggcac ttggtgatta tgtccatgtc tccttatacc
     9841 aaccagtaga tggcgaaaaa caatatgaag gatttcttca atcatttgat gctgaacagc
     9901 ttacattgaa aatacgcata aaaacacgag aaaaagaatt gacttttgac cgaaaaaata
     9961 ttgcaaaagc tcgcttagct atccaatttt aatcacggag aggaatcaga aaaaaatgag
    10021 taaagaaatg ttaaatgcgt tagacgcttt agaagctgaa aaagggatct caaaagaaat
    10081 cgtgatcgat gctttagaag cagcattggt ttctgcatac aaacgtcact atggccaagc
    10141 acaaaacgtc gaagtggaat ttgatcagaa aaaaggaaag atccatgtct atgcggtaaa
    10201 agaagtaacg gaagaagtga tggattcaca gttggaagtc tcattgaaag atgcactttt
    10261 aatcaatcca gcatatgaga tcggtgatac gatccgtttt gaagtaacac caaaagattt
    10321 tggacggatc gctgcgcaaa cggctaaaca agttatttta cagcgtgtac gtgaagcaga
    10381 aagaacgatt atttataacg aattcagtgc ttatgaaaaa gacatcatgc aaggaatcgt
    10441 cgaacgccag gataaacgtt atatttatgt caatcttggt aaaattgagg cagttctttc
    10501 taaacaagat caaatgccaa atgaatttta tcaaccacat gatcgaatca aagtttatgt
    10561 ttcccgagta gaaaatacgt ctaaaggtcc gcaagtattt gttagccgca gccatccaga
    10621 cctattgcgt cgcttatttg aacaagaagt accagaagtc tatgatggaa tcgttgaaat
    10681 tgtcagcatc gcccgtgaag ctggcgatcg ttcaaaagtg gcagttcgct caaatgatcc
    10741 aaatatcgat gcagtcggta catgtgtcgg gccaaaggga caacgagttc aagcaatcgt
    10801 caatgaatta aaaggcgaaa acatggatat cgtcgaatgg aatgaagatc cagctgtatt
    10861 catcgcaaat gcattgaatc ctgcacaagt catggatgtg atcttcgatg agacaaatcc
    10921 aaaggcctgc acagttgtcg ttccagacta ccaattatcg ctagcaatcg gtaaacgcgg
    10981 acaaaatgca cgtttagctg ctaaattgac taaccataaa atcgatatca aatcagaatc
    11041 tgacatgacg gaattttatg aaaatcaaga acaacaaaaa gaaacagaag aactgcatga
    11101 tgaagcaatc gttcagtcag atttaacaga cgatgaatat gaaacaattg cattcaacaa
    11161 tgaaacggtc gaagaaaaac cagaagctta actttagtaa ggaggcaaag agatgaaaca
    11221 aaggaaaatt cctttacgca aatccgtggt ttcaggtgaa atgaaaccta aaaaagagat
    11281 ggtccgaatc acccgttcaa aagaagggga agtatcgatc gatccaactg gaaaaatgcc
    11341 tggacgagga gcgtatgtct cacttgagcc ggaagaagta caacaagctt gggataaaaa
    11401 aattctcgat cgagtactcg aaacaacatt aacagatgag ttttatcaag aacttcttga
    11461 ctatgtcact caccaaaaag cacgtaaaga gttatttgga aaatgaatac agttaatcgg
    11521 caaaaagcca tgaatctcat cggtctagcg atgcgcgcgg gaaaactaat cacgggtgaa
    11581 gaactgacca ttgctgaaat ccgcaaacaa aaagcaaaat tggtgtttgt ggcaaccgat
    11641 gcaagtgaga atacaagaaa aaagataaaa gataagagtt catattatga agttccttgc
    11701 tttgagctat tttctgaagc agaaatcacg cagatgatcg gaaaacctcg caaggtattc
    11761 ggaataatgg atgccggatt tgcgaagaag accaaggagc taattgaagg ttaggaaggt
    11821 gattgcatgg gcaacaaaag aatctatgaa ttagcaaaag aactcaaaaa atctagtaaa
    11881 gacgtcgtgg aaaaagcaca acaacttggc atcgatgtga aaaaccatat gggtgcgatt
    11941 actagcagtg atgagaagaa attgcaacaa gcgttcaaaa acccaagcac gaaacaacaa
    12001 gctgcacaac aagcaagcag aaaaccagcc aatcaacagc cgaacgatca acgtaaaaaa
    12061 gaaacaacga acaatcaaca aaaaaataaa aataaccgca gttatcaaga tcgcggtcaa
    12121 gggagcggtc aagtgaatca aggaaaaaaa ccaacaacaa accaaaataa tacgggaaat
    12181 accagcagcc aaaatcgtca aggaacgagc caaggcggca atcaaaaccg tactaacaat
    12241 aacaacaaac caaacaataa caatcgcggc aaatttaata ataataaccg taatcgttat
    12301 aataaaaaag gtaaaaaagg caaacagcaa caatcaacta aaccagctgt accaccacgc
    12361 aaattccgtg aattgccaga tgtattagag tatactgaag gtatgaacgt agcagatatt
    12421 gcgaaaaaaa ttcatcgtga gccagcagaa atcattaaaa aattatttat gcttggtgtc
    12481 atggtcaatc aaaaccaagc actggataaa gatacgattg aactgttagc gacggactat
    12541 ggcatggaac cacaagaaaa agtccaagtt gacattgctg atatcgataa attcttcgag
    12601 gcagaagaag tcgatccaga taaattagtc tcacgtccgc cagtagtaac gattatggga
    12661 cacgtagacc acgggaaaac aaccttattg gatacattgc gccattcacg tgtaacaagc
    12721 ggcgaagccg gcggaatcac acagcatatc ggtgcctacc agatcgatat tgatggtaaa
    12781 ccaatcactt tcttggatac accaggacat gcggccttta caagtatgcg tgcacgtgga
    12841 gcaagtatca cagatatcac gatcttagtt gttgctgcag atgatggcgt catgccacaa
    12901 acagttgaag caatcaacca tgccaaagct gcaggcgttc caatcatcgt cgctgtaaat
    12961 aaaattgata aaccaggtgc aaatcctcag catgttatgc aagaattaag tgagtatgaa
    13021 ttaattcctg aagcatgggg tggagaaacg atttttgttg agatctcagc gaaattcggc
    13081 caaaacatcg aagaattgct ggaaatgatc ctgttagtcg cagaagtaga agatttaaaa
    13141 gcagatccaa cacaacgcgc aatcggaaca gtgatcgaag cacgtcttga taaaggtaaa
    13201 ggtcctgtgt caaccttact tgtccaagaa ggaacacttc acgttggtga cccaatcgtt
    13261 gtcggtaata cttacggacg tgtccgtgta atgacaaacg atcttggccg tcgcgataaa
    13321 gaagcaggac ctgcaacgcc agtagaaatc actggcttga atgatgtacc tcaagcaggt
    13381 gaccgtttcg tagtcttcga agacgaaaag acagcacgtg cagcgggtga agaacgcgca
    13441 aaacgtgcat tattggaaaa ccgtgctgcc acaagccgtg tgactttgga caacttattt
    13501 gaaagcctga aagaaggcga attgaaagaa gttaacgtaa tcatcaaagc agatgtacaa
    13561 ggttcagcag aagcactggc tgcatcattg aagaagattg atgtggaagg tgtccgtgta
    13621 aaaatcgtcc attcagctgt cggagcaatc aatgaaagtg atgtaacctt agcagcagcc
    13681 agcaacgcta ttatcatcgg tttcaacgtt cgtcctacac cgcaggcaaa agtgcaagct
    13741 gatactgaag aagtcgatat tcgtcttcac cgtatcatct ataaagccat cgaagaaatc
    13801 gaaactgcga tgaaagggat gctagaccct gaatttgaag aaaaaatcac cggtcaaatg
    13861 atcgttcgtg aaaccttcaa agtttctaaa gttggtacga ttgcaggtgc ctatgtaaca
    13921 gaaggatata ttcgtagaga tagcggtgtc cgtgtaatcc gtgacggaat cgtaatctat
    13981 gaaggccaac ttgcaagtct gaaacgattc aaagatgacg tgaaagaagt caaaatgggt
    14041 tatgaatgtg gtgcgatgat tgaaaaattc aatgacatca aagtagatga cgtaattgaa
    14101 ggatttatca tggaagaagt caaaaatgac taaaaactga taaaaggagg ccaatcatta
    14161 tggctaacta ccgtgatcgc cgagtcggtc aagaaatctt aaaagaagtc aatgatattt
    14221 tgcgcaaaaa ggtccgtgac cctcgtgtgg agaacgtaac gatcacagat gtgaaagtta
    14281 cgggggactt acagcaagca acgatctatt acagcatttt atctgactta gcatcagata
    14341 agaaaaaagc acaagatgga ttgaataaag catcaggatt gattcgtcga gagttagggc
    14401 acaatctaag tatttataag acacctgaat tgttctttga acttgatgaa tcagttgtct
    14461 atgggaacca tatcgatgaa ttgcttcgcg acttgaacaa agactaatca aaaaatcccg
    14521 caaatcctgt gaaatcaggg gttgcgggat ttttttgttt tcattaagac gtattaccta
    14581 agagttccat gactttagtc tggtagcgtg tcagctaacc aaaaaagaga aaaagaaaag
    14641 taaaaaattc gactttcttt tttctatagt atataaattt ttgcatatag ataaagatca
    14701 agataccgat aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag
    14761 taactagaag atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag
    14821 ttaaaagaaa aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc
    14881 acgatccaaa ttttggataa tccctgtaga aattagcata gtagctggga taccaattca
    14941 catattgaca aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc
    15001 tcgatccagc cccagttttc caacaatgtg ttaactggcc tagcgtcatt cagcaaatta
    15061 tgcatgatga ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg
    15121 gatcaatgta aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt
    15181 tggctagata taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt
    15241 atctgcgcta gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa
    15301 agaatacttt gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca
    15361 actttatctt aaagataacc ccttactgag acgtacactg aaaccagaag atgtcaaaaa
    15421 acatccaatc ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa
    15481 ccgagtcatc aataaatatg atttgaacat gttttatatc gaagggccag gacacggcgg
    15541 acaagtcatg gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt
    15601 aacggaagat gagacaggaa tgcaaaaatt attcaagcga ttttcatttc ctggaggcat
    15661 tgcttctcat gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata
    15721 ctctctttca catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt
    15781 gattggtgat ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt
    15841 tatcaatcca gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat
    15901 agccaaccca acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg
    15961 acttggctgg gagcctttct ttattgaagg aaatgatcca gaaaaattaa atcctgttat
    16021 ggctgaaaaa atggatcaag caatcgagaa aatcaaatcc atccaaaaag aagctcgcct
    16081 gaagacagca acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa
    16141 aggatggacc ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca
    16201 ccaagtaccg attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg
    16261 gctgaaatcc tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat
    16321 cagagaaatt gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg
    16381 aatcgatcca agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa
    16441 accaggctcc gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat
    16501 tgagaaaaat ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt
    16561 gaatcaagtt ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga
    16621 tgaatggctt tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga
    16681 agggttctta gaagggtacg ttctgactgg acggcatggt ttttttgcaa gttacgaatc
    16741 attcttgcga gtggttgact ctatgttgac tcagcacttc aaatggatac ggaaatcaca
    16801 tgacctttct tggcgaaaag attatccttc gctgaatctc attgcaagct caacagtctt
    16861 ccagcaagat cacaatggat atagccacca agatccagga atattgacac atttggcaga
    16921 aaagaaggcg gagttcatcc gtgaatatct gcctgcggat gcaaatacat tgctcgcagt
    16981 catggataaa gcattccgct cgtcagaaaa aatcaatttg atcatctctt ccaaacatcc
    17041 gcgtgctcaa ttttactcag cagaagaagc agccgtctta gtgaatgaag gcttgaaaat
    17101 cattgattgg gcttcgacag caaaagagga ggaacctgaa ttggtcattg ctgctgcagg
    17161 aacggaatct aacttagaag cattagcagc tgtaactttg ctgctggaag aattcccaaa
    17221 actaaaaatt cggttcatca atgtcgtaga tttattgaaa ttacgtcatc caagtcaaga
    17281 tccgcgtggg ttgagcgatg aagaatttga tcaatatttt acaaaagaca aaccgatctt
    17341 atttgctttc cacggttatg aaacattggt ccgcacgatt ttctttgatc gtcataacca
    17401 tcatttaatg attcatggct acaaagaaaa tggagatatc acgacaccat ttgacatgcg
    17461 tgtagtaaat gaacttgatc gttatcattt ggcaaaagac gcagctttaa aaatcaaagg
    17521 aagtcaagca gaagattttg cggaaaaaat ggatcagaaa ttacaagaac atcaaaacta
    17581 tattcgtgaa aatggtattg atctgccaga agtattggat tggaaatgga aaaatcttga
    17641 ccaataaaat gataaaatcg atcccgcaaa acagaatttt ttacattgta ttatttgtca
    17701 aattaag
//
LOCUS       DACESX010000048        17628 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.048, whole genome shotgun sequence.
ACCESSION   DACESX010000048 DACESX010000000
VERSION     DACESX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.048"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            118..618
                     /locus_tag="IXF88_001396"
     CDS             118..618
                     /locus_tag="IXF88_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1508135.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(808..1152)
                     /locus_tag="IXF88_001397"
     CDS             complement(808..1152)
                     /locus_tag="IXF88_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1508136.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1306..2226)
                     /locus_tag="IXF88_001398"
     CDS             complement(1306..2226)
                     /locus_tag="IXF88_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1508137.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            2413..2904
                     /locus_tag="IXF88_001399"
     CDS             2413..2904
                     /locus_tag="IXF88_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1508138.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(2928..5303)
                     /locus_tag="IXF88_001400"
     CDS             complement(2928..5303)
                     /locus_tag="IXF88_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1508139.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            5629..6255
                     /locus_tag="IXF88_001401"
     CDS             5629..6255
                     /locus_tag="IXF88_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1508140.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6278..6982
                     /locus_tag="IXF88_001402"
     CDS             6278..6982
                     /locus_tag="IXF88_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1508141.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7174..8007
                     /locus_tag="IXF88_001403"
     CDS             7174..8007
                     /locus_tag="IXF88_001403"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1508142.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            8204..10132
                     /locus_tag="IXF88_001404"
     CDS             8204..10132
                     /locus_tag="IXF88_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1508143.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            10270..10725
                     /locus_tag="IXF88_001405"
     CDS             10270..10725
                     /locus_tag="IXF88_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1508144.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            11195..11464
                     /locus_tag="IXF88_001406"
     CDS             11195..11464
                     /locus_tag="IXF88_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508145.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            11864..12118
                     /locus_tag="IXF88_001407"
     CDS             11864..12118
                     /locus_tag="IXF88_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1508146.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(12207..13253)
                     /gene="holA"
                     /locus_tag="IXF88_001408"
     CDS             complement(12207..13253)
                     /gene="holA"
                     /locus_tag="IXF88_001408"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1508147.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(13291..15564)
                     /locus_tag="IXF88_001409"
     CDS             complement(13291..15564)
                     /locus_tag="IXF88_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1508148.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(15557..16096)
                     /locus_tag="IXF88_001410"
     CDS             complement(15557..16096)
                     /locus_tag="IXF88_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1508149.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(16232..16450)
                     /locus_tag="IXF88_001411"
     CDS             complement(16232..16450)
                     /locus_tag="IXF88_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1508150.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(16447..16692)
                     /locus_tag="IXF88_001412"
     CDS             complement(16447..16692)
                     /locus_tag="IXF88_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1508151.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(16808..17536)
                     /locus_tag="IXF88_001413"
     CDS             complement(16808..17536)
                     /locus_tag="IXF88_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1508152.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5438 a         3388 c         3118 g         5684 t
ORIGIN      
        1 ttgttcgatg attcttaata cgatatgaca caataaataa aaataggcat ggctatttac
       61 aacaatagtg ctatagtaga aaacaaatga ataaggttac ttaaagaggt gaatacgatg
      121 aaactgatca aactatggga agcagattta aagaaagctt atcagctaca aactagcttt
      181 ggaccaaatg agagtggatt tatcaacaat gcttatggct atacatttgc tgaattttta
      241 gattatgtaa aaaaatgcga aaaaacgtct ttaggaatcg atcttcctga gagttacgtt
      301 cccgcaaccg tctttatttt agaaagtgat gaacatgagt atgtaggaat ttttaatttg
      361 cgccataaac tgaacgaaac attaagaaac ggcgctggcc atattggtta cggcatctcc
      421 cctaattatc gtaaaaaagg atatgccaca caaggattag cattagtact acaagaggca
      481 aaaaaaatcg gcattgatga ggcacttttg tctgtaaata aaaaaaatct tggcagtctt
      541 aaagcacaac aaaaaaatgg ggctgcgatt tatcgtgaag atgagaaaga atactatacg
      601 aaaatcaaac tgatgtaaaa aagaaggatt ccaaaaggaa agggatttga atgtccttta
      661 tcaatagaac acatttattt aattaactta taatatagca gacaaaattt tataaacaaa
      721 gaaacctttt atctcttttc ttctagaaag gataaaaggc ttttttgttt ttcctatttg
      781 cttctcatag ctagtcagcc aagatttcta tcgtatcccc aacgcaatcc gtgcataacg
      841 gctcatctta tctgtcgtcc atgctggata ccagactagt ttgacttctg ctttcgttac
      901 ttctggcact tcttttaatg cgtcatggat agattctgtt aaaatatctg ctaatggaca
      961 gcccatagtt gttagagtca tcttaatgac tgtttctcca ttttcaggat tgaattctat
     1021 atcataaatc aagcctaaat tgacgatgtc gattcctaat tcaggatcaa tcaccatttc
     1081 caaggcagca agaatacgtt ctttgattag ttctatttct tgtccagacc gttcttggtt
     1141 tgcttcgctc atcgtacgac actccttaac acgattttta tttatcatac tcttttttta
     1201 gctgcttgta aatgagaatt tagaagtaag attctcgttt aaatttataa aaatgttctt
     1261 attagatgta atagattaaa tttattctaa aataaacctg attcctcatt ttttttgtga
     1321 acgatttttt atttctggca atacgccagt tgttcgatac attttttgtt cgttttcatt
     1381 tgcaaaccgg ccaaatactg aatgagttgg taaaatattg ataaatgcat cgtcgtcgat
     1441 ttcatgaacg atctgatcaa gatcatacat ttcgtagcgg gtgatgacca tcatgatcat
     1501 gttcccttgt ttttttgaat agccaccgac agacggcagt aatgtcattc cacgtaccat
     1561 ccgttttgct agttcttctg ttacctcttc tggcttgacg gtaacgatca ttgctgttac
     1621 tttttgatga ctcgtgtgga tggcatcaat tacttgagtc attgcataaa tggaaataat
     1681 cgtatacaag gcactttccc agttaaagaa gaagcctgcg atgactacaa tgataccatt
     1741 tagaatcagc atgtagtttc cgactgtttt tccagttgtt tgtgaaagta caagagagat
     1801 gatatccatc ccaccagttg taaagcccat ttttaaagat aatcctacgc caagacctaa
     1861 taaaacaccg cctactaggg cattcatcaa aatattatca gtgatctgtc cgactggaag
     1921 cagcatcgtc atcaccgata cacatacaac attcacaaaa ctaaggatcg ttgctcgttt
     1981 tcctactttg agaaacccta atacaaacac agggatattc aaaagaaaaa taaatgttcc
     2041 tgtgtctatc ttgatatgaa accattcata taaaagcgag gaaatgattt gagcgatccc
     2101 attcatccct gcagaaaaga cattggctgg aatcaaaaaa taatttaaag cgaccgcagc
     2161 ggtcaatccg gttatcaaaa taaccactat tttctttaat ctttcatttt tatgatacgc
     2221 agtcataatt ttactcacaa gcacctctcc cctactttgg tattccaaat gtaagaataa
     2281 cagtttatct attaaaataa aatacttcga actcaaatga gaagaatttt agcaaacagg
     2341 taagaatctg ccctaatcct atgataagat agatgtatgg taaatattta tatgtatatc
     2401 aggagggttc ctatggcaaa agatgctagc ttcgacattg tatcagaagt caatacagaa
     2461 gaagtaaaaa atgcgattca gcaaacaaca aaagaattgt ccaatcgctt tgacttcaag
     2521 gattctacag tagaaatcaa attagagaac agcaatctag ttgtattgag cgatgatgat
     2581 ttcaaaatcg aacagatcaa agatgtatta tttggtaagc tgatcaaaag aaatgtgcca
     2641 atcaaaaata tccatttttc cgaatcagaa cacgctctag gtggcaaagc tcgacaaact
     2701 gccgatttgg tcaacggaat cgatcgtgaa aacgcgaaga aaatcactac agctatcaaa
     2761 aatgaaaaac tgaaagtaaa agcacaaatc caagaagatc agattagagt gacaggtaag
     2821 aaccgtgatg atctacaagc tgtgattgct ttattacgta aacttgactt accaatcgag
     2881 ttgcaattca ctaactatcg ttaatcaaaa aacgaggctg acgtctgtca ttcctcgttt
     2941 ttttgattta ctgcttcttt tgataaccaa aaacgctgta ttccttcttc caattcccca
     3001 acacttcgta cttctcttgc tgtttgccaa tgttgtggga ataaacgtcg ataagaaaaa
     3061 ctagaaaatc gttgatctgc caatacaacg attccttgat ccgccatgcc acgaatcact
     3121 cgaccaccag cttgcaagac tttgttcatt ccaggaagct gataagcgta agcaaatcca
     3181 aggtgttcct tctcatcata atatgatttg atcagttcct gctcatgatt catctgcggc
     3241 aaacctacac ctacgatcat actgccgatc aatcggtccc ctctcaaatc gattccttct
     3301 gaaaaaatcc cgccaagtac gcaaaatcca atcaatgttt cagcgggatt tgtttgaaat
     3361 tttgttaaaa atcgttcgcg ttcttcctca ttcatgtccg tttcttggat tagcacattg
     3421 ctgtcgggat attgctgtct gaatgcttcc accgcaagat ctaaatattg ataagaagga
     3481 aagaaaacga aataattccc gacctttcca tcagcaaaag catgaatcgt ctctactaat
     3541 tttgccagac tgtgttccct ttttcgataa gtcgtctcta ggtaatttgc gatgaaaact
     3601 tgctggtttc cttctgggaa aggactcggc agtttgtaag ccagactttt ttgtcccccc
     3661 agtgtttcct gataataaga gagtggtgaa aaactagcag aaaaaagaat actgcttttt
     3721 cctttatcca acgactgctc aagaaacaag cttggatcaa tacaaaactc ttttactatc
     3781 agatcgcgat acgttttttc gactgttgtt tcgtagtgat catcataaaa ttcactgacc
     3841 ttcaaaaaat gaagcaaatc aaaataataa ggaagcaatt gttcttgtga tgggtgctca
     3901 ggaaattctg ccagccattc tttaattttt tcgcttaatt gataacctgc ctttactaaa
     3961 gattcagccg gtgctttttg atgatgataa tcccaatgat cttcttttgc tatttctcgg
     4021 atcgaatcaa attcttttaa tagtttgttg aaacggcgat gaagtttttt gaattccttt
     4081 ggtatagctt ccttacttcg tttcgttttt tcataagata gttctgccga atacatctct
     4141 cgggaacgat tcaccaaatt atgtgcttcg tcaatcaaaa acagatagtc ctcgtttttt
     4201 tcatcttcaa aaaaacgtct taaataaaca gttggatcaa ataaataatt gtaatcccca
     4261 atgattacgt cacaccagat actgacatct aatgatagtt cgaaaggaca aagtgtatgc
     4321 tttcttgcat aagtttcgat gacttcacga gtgatctgat tctcatgatt caaaagatcc
     4381 cataaacctt cattaatccg attgtaataa ccattggcat atgggcactg atctggatta
     4441 caggtcgtct catctaaaaa gcagatcttg tctttcgccg tcaaagtgac actttttact
     4501 tcagctcctg tatcttttaa tgctgtcata gcatcttcag ctacttgtct agttattgtt
     4561 ttagccgtta gataaaagat cttttctccc tcttcttccc ctacagcttt caaagcgggg
     4621 aataacgtag agattgtttt tcctgttccg gtaggtgctt ctacaaacaa tttctgtttc
     4681 gtacggatcg ttttataagc agctaccgct agttcacgct gacctttcct atactcatca
     4741 tgggggaatt tcaaagccat caaagaggca ttgcgtacag tgcgccagtt ttcttgaaaa
     4801 acaagccatt ctttataatc ttcagtcagc tttttaaaaa aagtttctaa ctgctctctt
     4861 gtctgatgtt caacctttct cgtgatcact tcttctgccg tctggaagta agtcaattgc
     4921 agatttattt cttgtaaatc atgttcttga ctatagatat aggcatacac ccttgcttga
     4981 tgaaagaaaa gttccacttg ttctggttct aaatcttcaa atcgtggttc ggaggttttg
     5041 atttcatcta tataataaat accgtctctt ttgaaaatac cgtctgcacg gccttcaacg
     5101 atcaggtaat attcatctaa ttgaactgtt gtttttaaaa agacttcttt ttcatagttt
     5161 tctcccgcat ctttttggag cttgcggtgg attctcgctc cttctaatgc agtatggtta
     5221 ctttttttac gattgtcgat acttccacgt cgcaaaataa attctactaa gtgccgcaca
     5281 gatatttttg actgtttgat catttccacc acctctttct ctctaagatt acctgaaaaa
     5341 acctagttta gcaaaaagag ctctctttcc aaacataaaa aaacaccctg ctgattaggc
     5401 agggatatag gatatagaat ttggattttt ggatgaatct aatgtaacaa ggacatatta
     5461 aaatgctatg aaaacttact ttccttctct ttagaatatc caaatacttt tcatatagtc
     5521 tgataattca gataagtaaa gagaaataag tacctgtttt tgtttcctag caatgaatgc
     5581 gttatactaa tggatgaaag aagttttggc tatcaaggag gaatgcacat gcatatcgaa
     5641 agattaaaaa caggaatgat tgaagaaaac tgttatttgg tctacaacga agaagcactt
     5701 ttgatcattg accctggaga agatgcggaa cgaatcaaac aagaaatcaa aaaaacaaac
     5761 caacagccgg tagcgatttt gcttacccat actcactacg atcatatcgg tgcagtggaa
     5821 cctttacgcg cgtattatca aatcccagtt tatgtcagcc cactagagca aaaatggcta
     5881 ggtgatccga ttttgaatct ttctggactc ggtagacatg atgatattgc tgatgttatt
     5941 gtctcgcctg cagaatatga atttgaaatg aaaacttata ctttaggaaa catgtctttt
     6001 cgcgtcgtcc ctaccccagg gcattctata ggaagcgtca gctttatttt tgatgatttt
     6061 gtcgtttctg gcgacgccct gtttagagga agtatcggta gaaccgatct gtatacaggg
     6121 aatttagaac aattacttca tagtattcgg acccagctgt ttgtattacc tgatgaattt
     6181 gcagtctatc caggacatgg cgatgcaacg acgattgaac aggaaaaaag gacaaatcct
     6241 tttttcaatc gttaaaacga cttaggagga gaataaaatg acagaaacta cactttatat
     6301 cgtacgacac ggaaaaacga tgttcaatac aattgaacga gtacaaggct ggtgtgatac
     6361 acccctcaca aagcaaggac aagaaggcat tcattattta ggtcgcgggt taaaaaatgt
     6421 cgacttttca tttgcttatt ctagtgacag tggtcgtgca atcgaaactg cccgtattat
     6481 tctaggagaa cattctaagg gaaaagaaat tccttatttt attgaaaaaa ggattagaga
     6541 atggtgtttc gggtctcttg aaggtggata tgacatggaa atgtggggtg tgatccctcg
     6601 tgtattgaat tttaaaacat atgatgaaat gtttaccaca gaagtttctt ttgaacaaat
     6661 tgctaatgct gtatacaact tagataccgc tgggtgggcg gaaacttatg aaacattgcg
     6721 agaccgtgta tggacaggat ttgaagatat cgcccatcac tgcgaaaaaa atggtggtgg
     6781 aaatgtttta gtcgtatctc atggtttgac gatcgccttt ttgcttaatc tgatcgatcc
     6841 taaacagcct gttcgtgctg ggcttttaaa tggcagcgta acaaaggtca cttatgcaaa
     6901 cggtcgattt tctatccacg gcatcaatga tacgtcttat atcgataacg ggaaaacaca
     6961 tagtcgcgac tcgtttctat aatggggata ttttaatttt taccagagaa tcataaaagg
     7021 caaagtctga acgagggctt tgtcttcttt tttctcttcg tgtacttatc tgctttattt
     7081 ctcaagtttt tcttaatatt aaaaaacaga ttgggtttct caaaaaataa cggtatacta
     7141 taagatgtat cttaattggg aaggaaggaa tttatgtttt ttgcaaatat tatcaaagca
     7201 gtgattctcg gtattattga aggaataacg gaatgggtgc ccatcagtag tactggtcat
     7261 ttgatcctag cagatgaatt catcaaactg gatgccagtt cacaatttat gtcgatgttc
     7321 aatgtcgtta tccaattagg ggcgatcttg gctgtcgtcg ttctttattt tcacaagtta
     7381 aacccatttt ctccaagtaa atcaacggta gaaaaaaaag atacgtggac actttggttt
     7441 aaagtgatcg ttgcttgcat accagccatc atcattggtt tgcctttgga tgattggtta
     7501 gaagcaaatt tccacaagtt cttaccagtt gccattgttt tgatcgttta cggtattgcc
     7561 tttattattg tcgaaaaacg caacaaaaac aaaacacctc gttggacttc tctgaatgac
     7621 tttacgtacc aagcagctct tcttgttggg ttattccaag tcttggcact tgttcccgga
     7681 acatcccgtt ctggcgcaac gatcctagga tctatcttga ttggtgcttc gcgttttgtc
     7741 gctacagagt tttcattttt cttagggatt cctgtcatgt ttggcgcaag cggcttgaag
     7801 atcgtcaaat atttagctga agggaattca ttcaaaatgg aagaaactgt tgttcttctt
     7861 gtaggaacat tcgtatcttt tgtggtttcc gtcttagcta tcaaattctt actaagctat
     7921 ttgaagaaaa acgactttac cgcttttggc tggtatcgta ttatccttgg tatcattttg
     7981 attggttatt ggtttattgc aatgtagaaa aaaccagaac acttctctta tttgagaaat
     8041 gttctggttt tttattttct ttaataataa tcattataaa taataaaaac aacatcaagc
     8101 ttaatcacca aatgataacc attatcgttc tcatttagct gttattatta tcaattacat
     8161 ttttattatg ctatccttgt tttgacaagg aggtctaata acgatgacta atcagaaaaa
     8221 agcttttgca gcaacaatcg gctgcctttt atttatgatc ttcggtttta tcttcgaccg
     8281 attctctctt accatcaccc cactttttta tatcagtgcc attatcatcg gtggatataa
     8341 acaaacatcg gaaggcttga aagaattgtg gaatgatcgg acactgaatg tggatttatt
     8401 gatggcttta gctgctattg gtgcctgttt gatcggtaat tactttgaag gagcaatgtt
     8461 gacattcatc ttttgtttaa gcggtgcatt agaggaatac acaactaata aaagtcaaaa
     8521 agaaattact gctttgatga atcttcaacc acaaaaagcc caattattaa aagaaaatgg
     8581 acaaatgcaa gaagtagatg tttctcaact aaaggtggat aacttggtat ttgtcgctaa
     8641 aggagcagct gttccgatag atggcttttt agaaagcaca caggcaacga tcgatgagtc
     8701 tgcattaagc ggtgaatctg tgcctgtaga aaaatattta ggcgatgaga tgtatgcagg
     8761 aacgctgaat caaggaaatc ccatcacgat tcgagtaaca aaaacgagtt ccgaaactgt
     8821 ctttgctaag attatccaat tagtagaaga agcgcagaat acgccaactc aaacagcttc
     8881 atttatcgaa cgaatcgaaa ataattatgt aaaattgatt ttgcttgctg ttcctctaat
     8941 gatccttctc ccccactttt tcctaggttg gagttgggat gaaagctttt atcgaggaat
     9001 ggtattatta gttgtggctt ctccttgtgc gcttgtcgca tctgctacac cagcttctct
     9061 agctgctctt tcaaattctg caaaaaatgg gattttaatc aaaggcggta tccatttaga
     9121 aaagctttct gaattaaaag cgattgcctt tgataaaaca ggaacgctca ctaaaggaaa
     9181 accagttgta actgacagct ttttctttga tgaaaaggat ttggcacaac aacttcttgt
     9241 tgctatggaa caaaaaacga cgcatccact tgctcatgcg attttgcaat atttcgattg
     9301 cacaatacct gaagaaatcc aatacttacc aatagaagaa attactggtt ttggattaca
     9361 aactacctat ttaggtgcgc aatggaaagt tggcaagcac gcatatgatc ctgagacgat
     9421 gataatcagc aaagagattg cggaaatgat cgaacgtttg gaaaatcaag gcaaaaccgt
     9481 gatttatctt tctaaagatc agcagttaat tgctgtttta ggtctgctag atattccaaa
     9541 agcaaatacc caacaagtaa tttcctactt taaatcccaa aacatccata caagtatgat
     9601 aaccggcgac cattctggca cagcaaaggc cattgctgaa caagtaggga ttgaacatta
     9661 ctatgcaagc tgtactccgg aagaaaaaac acaactagtc aaaaaagaga atgaacttta
     9721 ccaagtaaat gcgatggttg gtgatggagt caatgatgcg cctgctttag cagccgcttc
     9781 tttaggtgtc gctatggggc aagggacaga tgccgctatg gatatcgcag acatcgtctt
     9841 aatgaaaagc gacttaaata aatttgtcta cagtcatcgg ctatctttaa aaatgcggcg
     9901 gatcatcaaa caaaatatta ttttttcgat tcttgttatt ctcttgttga ttgcttctaa
     9961 cttcctgcaa tttttaaatc tacctcttgg agttgtagga cacgaaggaa gcacaatatt
    10021 ggttattctg aatggcttgc gtttactaag acctttacct gatctttctg attcttcaaa
    10081 aaaatgtaag tcttgtccac tatacaaagc gcggctgtct tctgtcaaat aagcaaagtg
    10141 tttttttatg attttgttta ttttatgaaa aaaacagctt atgaataggt cttgaaggat
    10201 gaattcctac ttttgtaact ttctaaaaac atgttatgat tgtttcgaag aaagtgagga
    10261 atatatttca tggaaagttg gcttttttta ggaattattt tacttgtagc aattttagct
    10321 aaaaatcaaa gtctgatgat agcggcagct tttgttttga ttatgaagct cataccagcc
    10381 tcccgttcct ttttcccttg gcttcaaggc aaaggaatca atattggggt gacgatcatc
    10441 acagcggcta ttctaatccc tatcgcaaca ggagaaatcg gtctacgtga tctattagaa
    10501 gcctttaaat cccctattgg ctgggtagct gttttttgcg gtgggctagt ggctgtctta
    10561 tcttccaagg gtgtaggatt gattgcttct gatccacaaa tcactgttgc acttgttttc
    10621 ggcacgattt taggtgttgt cttcttaaga ggaatcgccg ccggaccggt cattgcttct
    10681 ggtatcactt actgtatctt acaagtattt gctttaggaa aatagaacaa attttgtcta
    10741 ttttaggaga ttcttaacac ccccaattta ctgatccgtg atatactgac aacgaggtga
    10801 tacaatgttc gaaaaattaa agtatttcat aggaacaatc ggctggtttc ttctagcggt
    10861 acttttgatt ctgggcttag cttatctatt atttcctgat ttgatacaaa atgtgtttga
    10921 tactgctttt tgattgacct attaaaacga tataggggat gctcctgcga tgcaggggct
    10981 ttttttaatc aatgttttgt aataatttag gccgtgccaa tgagacttcc tatagaattc
    11041 gatgctcttt tttctttttg gctcttctgc tcttttttgt tatactagat tacagtggac
    11101 aaaagaaaga atgatcgcat ccacttcaac gaatatcaaa atggaagaat tagtgcctgt
    11161 tttcaggtta ctttgaaaag aaaggaagac gcttgtggaa acttttgaag gtcttttaat
    11221 tgatacgtac gttttacaag aacagccaat gatgctgact gggatcaact tgcagcttga
    11281 ggaccgtgtg atcaatggcc tgcttaaaga tcgctatttg gcaaaatata tcttacgttt
    11341 gcccctaaat aaatatgtgc tggaagtaag tggcaaatgg aaccgtcgga accagttggt
    11401 cataaaaaaa atgttgataa aaaatatcga tgaatacatt aaaataatcg gtacaccaga
    11461 atagccttta ctgatcaaag gatactggtt ataaaataac tgcagtttct taatagaaaa
    11521 acaaagaaca acagggaatc gtatcccttt tcaaaaaaaa ctttgcaaac ggttctcttt
    11581 gttatataat aacttagtac caaacattcc cattcaaact ttgtttaaat agataaataa
    11641 aaatctagtt tggtttgacg ctattgcctc ccacaatagc gtctttttga tttttttcag
    11701 cttgtactat ccgtttttac tgaaaaaaaa tcctaataaa agactgttaa ggaaaaaaac
    11761 ttgtcatgtt catattgcaa agcacgctgc ctttaggtat aataatgaat gttgaattat
    11821 gcggtaacac gcgtaagaac ttaagttatc ggaggtgaaa ctaatgccaa acattgaatc
    11881 tgcaatcaaa cgtgttcgta caagcgaaaa cgctaatgtg aaaaattcat ctcaaacaag
    11941 cgctatgcgt actgcaatca aaaaatttga agatgctgta gcttcaggtg ctgacaacgt
    12001 agatgcgtta tataaagaag ctgttaaagc tattgatatg gctgaatcaa aaggtctaat
    12061 ccacaaaaac aaagctaacc gcgacaaatc tcgtttaagc aaaaaaatcg ctaaataaaa
    12121 gaatcggctt cggccgattt ttttatttgt caaaaaaagg acaaaaacaa acgaccttcc
    12181 cgcatgcttt gccctcgtct ttcttcttat cgatttctta acaactgccc tgtgcgcaaa
    12241 acgaatagtt gaaaacttaa ttctttttcg atttgtcccg ttttgatttg ataatccatc
    12301 tcaactaatt gatcaaacaa ctgtaccagc aaatgttcat cgaatttttt tacttcctgc
    12361 atggcaagtt ttacacgata tggatggatt ttcaatgttt cagcaatatt tgcctgctga
    12421 taaccaatct ttacaagaaa ttttgtctgt aaaaataaac gtaattgaga aagcaaaatc
    12481 gcattaattt tgattgtttc ttccccttgc ataactaaat cttcgtataa acgcaaagct
    12541 tgttccgtct tcccttttaa aatatattgg gtcatgtcaa aaatattatg ttctaacgtt
    12601 ttaggaacta attcttctac ttctcttacc gtgatttgct gttgattttg tccataaagg
    12661 accaatttct gtaactctct catcattttg gataaatcta agtcggtcaa cctcaagaat
    12721 aaatcaattg cacttcgttc cattttgatt gaggaattct ctaaggtatt catcagatat
    12781 tgacgtactt ctttttcatt caactgttgg acattgatga taattgctgt ttttttcaat
    12841 tgtttcgtta cttttttccg ttcatccaac ttttcatatg gcgcaaagaa tactaataca
    12901 gtcgattcta atggttcttt caaataagcc gtcaataaat cagtattttg ttcgattcca
    12961 ttgtttactt tttcacctgt taaaaaataa ggattctcta cgaatactaa ccgttgatct
    13021 ccgaaaaacg gcaacgtctc tgcttcagct actacctcat ctaaacttgc attttccatg
    13081 tcaaaactta aaaaatttaa gtcatcttcg ccctctaatt ggattttttt gacaatttct
    13141 gtccgaactt gatcttggag aaacttttct gttcctaaaa tcaagtaaca agatgcaagt
    13201 ttttctttac gtattttttg taactctgct tgtatactca aattactcac cactttcaaa
    13261 aattatccta ccatgagcag atgagaatta ctagtctatc attgtttcca ctagcccatg
    13321 aggatgaaaa actggatgcc agcgataggt gatcattcct tgttgatccg ttcgccatgt
    13381 ttttatctga ttttcttcta gtgtctccaa tacttcgctg tgcgggtgac cataacgatt
    13441 atttacgccg caggatataa tcccatgttt tgcttcaata tgggaaagaa aatctgtgtt
    13501 acttgacgta cggctaccgt gatgacctaa tttgactaca tcggctttca gattaggata
    13561 aatcaaaagc aattgttttt ccattgcttg attcaaatct cctaaaaata aaaagcgcct
    13621 attgttatat tccatccata gaccaatcga atcttcattt cttccctctc ccttttcaaa
    13681 aggagtcaat acataaagat cataataacc gctgatcctg tccccatgtt tgatttcaat
    13741 gatctcagta ccattttttt gagcttcaga taaatgcctt tgtaatggct catttcgtcc
    13801 agcgccccaa ccgatataaa tttttttgat agtaaaatgc tgagataacg tgggtaattc
    13861 tcccacatga tccgtatcgt cgtgtgtcaa gatgagttta tcgattacac ttatcccctg
    13921 accttttaga tatggaacga tatttctttc agcaggagga cgtatcatac cttgcgccca
    13981 cttttctctt tcaaagtgta actgacctcc agtatcgatc aaaatcgttt cttttcgaaa
    14041 aggactttgc aaaaagatac tgtctccttg accgacatcg ataaaagcga tcgttgaaga
    14101 aaagcgtaaa aatggcaaag tcaaaaaaat aaaagaaaaa agaccaacat aaaacaagtg
    14161 tttcacaggt tgttctttca acctatccat acaaatcagt aatccgaaaa tatacatccc
    14221 agatagcaca aggggcaaac gtcctataat gagagacgac cctgaagaaa aatcgattat
    14281 tcgctcaaat aagattaatc cctcttctat cagcaaaagt aaagtttccg gcagccagcg
    14341 attgaagagt gtgagaaata acacaccagg aagaaaaaga aacttgaata ccggtgcaat
    14401 cagtaatgta aaaagactac ctaaaatagg ccactcataa aaatgccata caatcaacgg
    14461 catcgtgatc atagtaaaca ggacgtgaga ggccgttttt ttcaaccaat ctccatcaaa
    14521 agaaatcagg ataaacaaaa aactcatgaa aaaactaagc tgtccacccg catgaaaaag
    14581 aaatcgagga tcaaatagta aaagaagcca aaagacaact gcaaataagt ccatggtgga
    14641 ataatgctta ttcagcaatt tgactagcaa tcgcaataaa aacagcaaac tagcacgcaa
    14701 aacagaaata gaacttcctg caatagaaac ataacagata gttagtagac tcaacggaat
    14761 aatactgacg tgtagtggcc atccgatacg tcgaaataaa taatacagcc accctaaata
    14821 aaattggatg tgtagacccg atagactgaa cagatgcagc aaaccactct ccctaaaaat
    14881 ttcttctggt tctctgaatc ctgcatcttt atagccccat aataaagaat taatatatac
    14941 acttgttttt tgcggaaatt cagattggac aaaggagata aaagatcctc tttttctctg
    15001 aagccagttt cgccagtgat tttgcatggt gatctgttca atcttttgaa tttggaaccg
    15061 tccagaaatt ctatggttcc tgaagtaagc ttcttggtca aaattataaa gattccgctt
    15121 ttttgctgga gagatgaact cgccttcagc agtcaattct attccctttc ctcggaagtt
    15181 aagccatgtt ttcaattctg cttctgtgtt tacttgataa gaaagctgga gcttttgttt
    15241 attctctgat aaccttcctt ccattgtcaa aaaattacca tttgcacgaa tcgtatctgt
    15301 aaatacagta atttgcgcag atgttgtttt ttcctctaaa aatgaatact cttgtagcct
    15361 ataagaaatg gataccgtta tgctacacaa aatacacaag tacgcgataa aacgtccttc
    15421 ttttagcgaa aaccataaaa aaattccggc aagaagcaaa cgataacccc aattgtcagt
    15481 gtaatacaac aaacagataa tcatcgacgt aattgccgga aaaatcaacc gatttcgaac
    15541 atgcctttct aaaacgctag acatgttttc ctgttaattc attttgaagt tttgaaaaat
    15601 gctgtgagtc taaagcaact tggtgtacag atacacctaa ttgttcgatc aacgcttggg
    15661 cataaggatc atttcgatag tcatggagat aattgatccc tttcacacca gcttgcaaca
    15721 aagcctttgt acaggccaaa catggaaaat gtgtgacata gatttctgaa ccttccgtcg
    15781 ggataccaag ttttgcacac tgtaggagtg cattcatctc tgcatggatc gttcgcaagc
    15841 agtgtccatc cacgacgtaa cacccttcat ctatgcagtg gacatctcca ctgactgctc
    15901 cattatagcc gccagcaatg atcctttttt ctcgaacgag tgtcgctcct acttctaaac
    15961 gtgtacacgt actacgtaaa gataacagta ctgcctgagc cataaagtac tggtcccaag
    16021 gaatgcgttc attatgtgac ttatcagtaa ctttttcttt attcgtatcg catgcttttg
    16081 aagatttttc taacatgttt tatattcctc cgttttttca agttgattaa aaagtactca
    16141 ttgcttaaac aaaatattct ttaaatataa cgaatcatcc tgtctgacaa aagatcctac
    16201 ccttcactgc cgaaatgaag tccgtcatcc attagcccat tataaaaatt cttcgaatcc
    16261 atttgttcaa gaaattccgt ataaaaatcg atacttcaca atcatggcgt tttgctactt
    16321 ctgtgagaac agagatatat tctttgatca acttgttatt ccgatatttt tgttttgtct
    16381 catcgactgc tggtggcgtg ataaagaaag catgatcaga actatagtct tttttcagct
    16441 gaactatcat ccactgcaga ttttttggta cgtttcttta gaaagttgtt tatgctctgc
    16501 tagatcattc gtcccaatga gaataaatac tttatctgct gtatagctaa gtacatcttt
    16561 ggcaaagcgt tctcgtagag cttgactgtt attgccgctg acagccgtat tcataatatg
    16621 aatcccagat attttatttt ggagatagac attcagcatt ggttttttta atgtttcttt
    16681 gcgaacaatc aagctatttc ctatccaaaa atactacctc ctcgtcctgc caagctctta
    16741 gaattggatt attcttagta tccattagcg tccatttatt gctcgtgcca gtcgattttg
    16801 aatcattcta tttaattgta acgaagtttt ttagtttttc aactgtcttt tcaccgatac
    16861 cagaaatctc ctgcaggtct tccactttct ggaaggatcc gttttcttct ctaaatcgaa
    16921 tgatttcttg cgctttgact gatccgatcc caggaatctg ctgaagttct gatggagtgg
    16981 ctgtattgat atttattttg ggaggctcgg aatctgctgt aggctgtttt ccttccgagg
    17041 atgccaactg atcaagatgt tctggcagct cttcacctac agcagggata tataggactt
    17101 gctgatcttc tatcttcgca gaaaagttga ttcgatcgct atccgcttct tctaataatc
    17161 cacctgcacg ctttagtaca tcatagaccc gttcttctga agaaaaggaa tagataccag
    17221 gctccttcac acttcctttg atatcggtgt acatgacctt tggacgagca tcctcttcta
    17281 catcgtttcc ttcagctgat ggatcttctc cactggaact aaacgaagaa agtatttctt
    17341 ttgcagcagt ctcttctgtt tctctataaa atagcaaatt tgataataaa aaaccacttg
    17401 cgatacaaaa gataagaact atcatagaca tccaaatgat caatttgggt gaagctagta
    17461 ttttcttgct gattttttct atcattgttc ttttttcaaa gaatgaccag taaaaatcat
    17521 tcattactcc tttcatttaa aattgaagaa acagtgagct ctggcaaaat cgtcccttca
    17581 acatctaatt acgtcaattt tttcttttgt ttttattttt atcgtgaa
//
LOCUS       DACESX010000049        17073 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.049, whole genome shotgun sequence.
ACCESSION   DACESX010000049 DACESX010000000
VERSION     DACESX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.049"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(40..2490)
                     /gene="parC"
                     /locus_tag="IXF88_001414"
     CDS             complement(40..2490)
                     /gene="parC"
                     /locus_tag="IXF88_001414"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1508153.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(2502..4547)
                     /gene="parE"
                     /locus_tag="IXF88_001415"
     CDS             complement(2502..4547)
                     /gene="parE"
                     /locus_tag="IXF88_001415"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1508154.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(4713..5147)
                     /locus_tag="IXF88_001416"
     CDS             complement(4713..5147)
                     /locus_tag="IXF88_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1508155.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5274..5912
                     /gene="plsY"
                     /locus_tag="IXF88_001417"
     CDS             5274..5912
                     /gene="plsY"
                     /locus_tag="IXF88_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1508156.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(6044..6919)
                     /locus_tag="IXF88_001418"
     CDS             complement(6044..6919)
                     /locus_tag="IXF88_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1508157.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(7001..7792)
                     /gene="codY"
                     /locus_tag="IXF88_001419"
     CDS             complement(7001..7792)
                     /gene="codY"
                     /locus_tag="IXF88_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1508158.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(7810..9210)
                     /gene="hslU"
                     /locus_tag="IXF88_001420"
     CDS             complement(7810..9210)
                     /gene="hslU"
                     /locus_tag="IXF88_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1508159.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(9224..9772)
                     /gene="hslV"
                     /locus_tag="IXF88_001421"
     CDS             complement(9224..9772)
                     /gene="hslV"
                     /locus_tag="IXF88_001421"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1508160.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(9809..10714)
                     /gene="xerC"
                     /locus_tag="IXF88_001422"
     CDS             complement(9809..10714)
                     /gene="xerC"
                     /locus_tag="IXF88_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1508161.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(10912..12288)
                     /gene="trmFO"
                     /locus_tag="IXF88_001423"
     CDS             complement(10912..12288)
                     /gene="trmFO"
                     /locus_tag="IXF88_001423"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1508162.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(12257..14335)
                     /gene="topA"
                     /locus_tag="IXF88_001424"
     CDS             complement(12257..14335)
                     /gene="topA"
                     /locus_tag="IXF88_001424"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1508163.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHGHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(14457..15314)
                     /gene="dprA"
                     /locus_tag="IXF88_001425"
     CDS             complement(14457..15314)
                     /gene="dprA"
                     /locus_tag="IXF88_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1508164.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(15370..16137)
                     /locus_tag="IXF88_001426"
     CDS             complement(15370..16137)
                     /locus_tag="IXF88_001426"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1508165.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(16130..16990)
                     /gene="ylqF"
                     /locus_tag="IXF88_001427"
     CDS             complement(16130..16990)
                     /gene="ylqF"
                     /locus_tag="IXF88_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1508166.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         4350 a         3812 c         2968 g         5943 t
ORIGIN      
        1 taatacagat ggcgtctctt cattaaaata ctacaacagt tacgactcga tgaaaatctc
       61 aggagcatcg ataacttcga tgacttctcc accttttttc tcatccatcg caaatgatcc
      121 attcgatgtt cgatcaccta tagggaaatc agttgggtca atcgttaact gctgcccttt
      181 ttgattcatc aaggttattt cttttgatct tccttccgac atatagataa tacggtgcgg
      241 attttttttc agctcacgca tgaccgtcac tccccgcttc gcacgtgaag tttggctgat
      301 ttcttgagca agcatgcgtt taacagctcc tcgctgagta acgattacga tcggcgtatc
      361 accttctgct aacacaagca gcccgttgat aatcctatct ccatctttca aattcataga
      421 cttcactcca gctgcctttg ctccaacaac gggaacatct gttaaaggat agcgcaaacc
      481 cattccttga tgcgttacta agaaaacatc taagcctggc tgatcttctt tcagatacac
      541 agcaatcagt tcatcttctt ccgcctttag cttcatacag ctgagcggac ggctcttata
      601 ggtgcgccat ggttcgaact ctgtcattcg tgtttgcttg atcaatccat tcttgctgat
      661 gaatacaaat gttttctctg cttgaatttt ttgatatggg aaaacagcta aaatcgattc
      721 gtctacagac agattggaga ttgtttgaga aatgtgctca ccagcatctt tccatttcaa
      781 atcgggtagt tcatggacag ggcggtagat cacatttgct ttatttgtga tcagaagaac
      841 atggtccaat gtactcaatt cacctgagta tagcaagtag tcgccttctc gcatcccgat
      901 ttcttctggt ttggaagcac tataagaacg aaggcttgtt cgcttcacat agccttctcg
      961 cgtcacactg acaattacgt cttcttgtgc cactaagact tgcgtatcaa ttttgatttc
     1021 ttcgatttcg tcttcgattg tcgttagacg agcggaagaa taattttttt tcacttctcg
     1081 caattctttt ttcataacag agaaaagctc tttgtcattt gaaaggattt tattcaactc
     1141 tgtgatttgc gcaatcaagc tttcggattc cttttgaagc tctgtgatat ccgtgttcgt
     1201 caaacggtat aattgcaatg tgacaattgc ttctgcttgt tcttctgtaa attgaaagac
     1261 gtcgactaaa ttctttttgg cgtctttttt gtctttactt tcacgaatcg ttgcgattac
     1321 ttcatccaga atcgataaag ctttcatcaa tccttctacg atatgctgtc ttttttgagc
     1381 tttggctaaa tcgaattgac tacgttttgt tatgacttgc tttcgatgct cgatataact
     1441 tcttaaaatg tctttcagtc caacttgatg cggagtcata tggtcgatag cgaccatatt
     1501 gaaattgtaa ttgatctgta gctcagtgtt tttgaacaaa taattcaaaa ttccttgagc
     1561 attggcgtct tttttcaatt cgacgacaat ttgcagacct gttctatcac tttcatctcg
     1621 tacttcagct attccatcga ttttcttatt caaacggatc tcatccattt ttttcaccaa
     1681 ggtcgcttta tttacttcat atgggatttc tgtgatcacg atttgctgtt tattaccctt
     1741 cattgcctct atctttgttt tcgaacgaag aataactttt cctcgtcctg tttcataagc
     1801 ttttttgatt tcatcttttc cttgcaagat ccctcctgtt ggaaagtcag gtccaggaat
     1861 atattccatc aatttttcaa ggctcgcttg tggatgatcg atcatataga ttgtgccatc
     1921 gatcacttca gctaagttat gagtcgggat ttctgttgca tatccagcag atatacctgt
     1981 cgatccattg acgagaaggt ttgggtatcg agctggaagg actgttggtt ctttttctgt
     2041 atcatcaaaa ttccacacga gatcgactgt ttctttctcg atatctgcta gcatctctcc
     2101 acttaacttg gataatctgg cttcagtata acgcatcgca gcaggtggat caccgtccat
     2161 actcccgtta ttcccgtgca tttcgatcag tacttcccgc aacttccagt cttggctcat
     2221 gcggaccatc gcttcataaa tactgatgtc accatgagga tgataattcc ccatgatatt
     2281 tccgacagat tttgctgatt tacggaatcc tttgtcatac gtattgccat ctttgttcat
     2341 ggcaaataat atccggcgtt gcactggctt caatccatca cggatatcag gcagtgcacg
     2401 ttcttggata atatatttgg aataacgtcc gaagcggtca cccatcactt cttctaaagt
     2461 taattcttgt acttcttgtc gattttccat caggtgacct cctattctac ttcaaataac
     2521 tgattgtttt catttttatc tgctcgttct tcatctaata aatcattaga aactgaagga
     2581 gagatttcag tatcctcttt tttatctaag atactgccat cttcttcgag cgtaaattgc
     2641 acgtgatttt cgatccattt tcttcgaggt tctactttgt cacccatcag tgtggttact
     2701 cgacgttcag cttgcgcagc atcatcgata cggacgcgga tcaacgtacg ggaagtcgga
     2761 tccatcgttg tttcccacaa ttgttccgca ttcatctctc caagtccttt atatcgctgc
     2821 agcatatatc ctttgccgac ttttttaatc attgctgcca attcgtcatc tgtccacgca
     2881 tattcaatga cttgcttctt tccttgtcct ttggatactt tatataaagg cggtaacgcg
     2941 atatatactt tcccagcttc aatcagcggt ttcatataac gataaaagaa agtaagcagt
     3001 aatacttgaa tatgggcgcc gtctgtatcc gcatcggtca tgatgattac tttgtcgtaa
     3061 ttacagtctt cgatagaaaa ctcaggaccg actcctgcac caatcgtata gatcatcgta
     3121 ttgatttctt catttttcaa gatatcttgc attttagctt tttcggtatt gatgactttt
     3181 cctcgtaaag gtagaattgc ttggaacttt ctgtctcttc cttgtttagc agatccgcca
     3241 gcagaatccc cttcgactaa atacaattca tttttcttag ggtttcggga ttgtgcgggt
     3301 gtcaattttc ctgaaagaag agattcgcct tttttacgct tcttcccatt tcggctttct
     3361 tctctggctt tacgtgctgc ttctctcgct tctctggctt tgattgcttt acggaccaac
     3421 atctggctca tttcgctatt ttcctgcaaa taaaagccca tttgttcact gatgacgtta
     3481 tctactacgg tacgagcaac tggcgtacca agtttttctt ttgtttgccc ttcaaattgc
     3541 agaagatttt caggtacacg gatagaaagt acagcagcta acccttctct gaaatcacta
     3601 ccttccagat tcttgtcccg ttctttcagc aaaccaactt ttctcgcata ttcattataa
     3661 gatttcgtca tcgccgcttt cataccagct tcatgggttc ccccatcttt tgtccgaacg
     3721 ttattgacaa aagataaaac attttctgaa taaccgtcat tgtattgata agcgacctct
     3781 acttcgatac cttctttttc tcctgaaaaa taaacaactg gtgtcaatgt atctttttct
     3841 tcattcagat aatcaacaaa ttctttgatt ccttcttcgt aatgaaaaat ttccttgact
     3901 ggttcttctc cacgaagatc agaaagagag attttaacgc cttttagaag aaaggcagat
     3961 tctcttaacc gttcggctaa aatttcatat gaaaatttcg ttgtactgaa tattgtatca
     4021 tctggtaaaa aatggacagt cgtcccgttt gctttttttg tttttcctat ctttttcaaa
     4081 gtaccgtcag gtttaccgcc gtttttaaat ttctgctgat actctacgcc atctctaacg
     4141 atcgttacaa ttagccattt cgataaggca tttaccacac tggctcctac accgtgcagt
     4201 ccaccagaag ttttgtaccc tccttgacca aactttccgc cagcatgcag cacagtaaaa
     4261 atcacttcaa ctgttggtat tccggaagca tgcattccta caggcatccc acgtccgcta
     4321 tctgcaacag ttatactatt gtcttcatgg atggtcacat caatctcact gccataacct
     4381 gataatgctt cgtcaactgc attatcgacg atttcataga ctaaatgatg caagcctcgg
     4441 ctgtcagtgg atccaatgta catccctggt ctttttctaa ctgcctctaa tccttccaat
     4501 acttggatgg acgagtcatt gtattcgttg tttacttttt ttgccaaggc aaacgctcct
     4561 tcaataatac tttgcggcaa aaccgcacca cgattatcat actagaaaag gtcgtgaaaa
     4621 aaaagagggt agacgagaaa tcaattttaa ttatctccta accgcttttt cgacattccc
     4681 ctctatcgat catctcttct ttgtggtcat ttttattttt ggttttcttt caacttacca
     4741 agttcaatct taatacaacg atccattacg atatctgtac gtcctgcttt tcttagcatc
     4801 tcagctgctt ctttgttttc aatccctaat tgtgcccaga acactttagc atcagttttc
     4861 aaaaaatcac ctgctatttc aggtaaaaat tcactccgtc taaatacatc tacgatatcg
     4921 attggatcat cgatttctgt caaattcgca tagactttct cgcctagaat ttcttcacca
     4981 gccaataatg gatttactgg tatgatttta tatccattgt cttgcaatac ctgtgcaacc
     5041 atgtaacttg ttcgttctgg tttattgctc aagcctacga cggcaatatt ttttgcttct
     5101 tccaaatagt tgaaaatcgt ttcttgtgtt ggatttttta cagacatttc tgccacccct
     5161 tgcttttctt tttattccat ctattatact tgtatctttc attttttcaa ttgttcctgt
     5221 tacctattca tgctaaaata aaggatagtt ttcaacttga aagagggtaa aaaatgaaat
     5281 tgatcttatt acttatttta gcatatcttt taggttctat tccttccggt gtctggattg
     5341 gcaaattgtt tttcaaaaaa gatatccgac aacacgggag cggtaatact ggaacgacca
     5401 atacatttcg tgtattaggc aaaacagccg ggattgttgt cctgcttatg gatattttaa
     5461 aaggtacatt ggctacttgt ttgccgatga tcttccacct ttctgggatt aaccctcttt
     5521 ggtttggggt atgcgccatt cttggccata catttccgat ttttgctggg ttcaaaggag
     5581 gaaaagctgt tgcgacaagt gcaggaatgc ttttaggatt taatcctgta ttttttgttt
     5641 attcttgtat catctttatc gttagtttat tttgtacaag tatggtgagt ttgagcagta
     5701 tgatcagtgc agtattgatc actttatcta cgatcgtcct tccttatgta gctcctgtga
     5761 ttttggctaa gcccaattgg ttactgacga tcattgcctt tttagtttct tcctttatct
     5821 tttatcggca taaagacaat atcaagcgta tcagaaacca tactgaaagt cgaatcccgt
     5881 ttggtttgaa tagcagcaag aaaaaatcct gatacttttt cattccaaag agataaaacg
     5941 ggagtgtgac aaatcgatgt cacactccct tattacttac aggatagctt ttttacacac
     6001 cttggtaaac ggtgttccct gctaacttct cggtcacaac ctcttacttc actgtgatgg
     6061 aatattttgt cttgaagatt tcatgggcac ctagttgatt gatccctact ttttccatca
     6121 agtttccact actgttcacc gtgtcagcga ttccgcacca cggttcgata caaacgaaag
     6181 gcgcttcttt tggataagga gaccagatcc ctacataagg catatcttta tagcttaaag
     6241 ttacgctatg ggaagatttt tcacttcgaa tggcgtacgc atttagaccc cttgtttcaa
     6301 aaaccaaggc atcttgctca aaaagttcac gattcaaagc cagattcgta tttgtctgac
     6361 ctaacgtctt ttgatccata tcgatgaatg ggcctttcaa tggtaggcgt aatcttgatt
     6421 tcaatggaga aaatgccaga tagaaatctt caaatgacaa tccttcttca agaggtacat
     6481 tgaaagcagg atgtccaccg atagaaaaga acatttcttt ttcaccggta tttgccactt
     6541 gataatgaac cgtgatgccg tctcctccta attcatagct gatcactaat tcaaaatcaa
     6601 atggatactt ttctaatgtc tccggcgtac tccttaagat gaaactagcc atttcttgtc
     6661 catgttcaat gacttcaaaa tccatatccc gagcaaatcc gtgttgcccc atttgatatg
     6721 ttttcccttg ataaacatac tgatcatctt ttaaacgtcc aacaaaaggg aataaaaccg
     6781 gtgcatgtct gccccaatac gctggatcac cttgccagat atactctaaa ccatgttctt
     6841 ttgatttcag actgatcaat tccgcaccgt gttcagcgat ttcggcgatc agctggtcat
     6901 tttgaataat aacagtcatt gccattcctc caacttcgac ttacttttta aaatgggaag
     6961 gctggaacaa acatttctat ctgccccagt ccccttactt ctaataactg tgttttgcta
     7021 actcgtcttt aaaacgggaa ttcaatactt ttaaataagt tcccttcatg cctaacgaac
     7081 gagattcaat gattccagct gattccaatt ttcgtaaagc attgacgatc acagaacgag
     7141 tgatgccgat actatcggca atattagaag cagtcagacg accttcatct ccatccaatg
     7201 cttcaaagat agcttgtaca gccttcaatt cgctataaga taatgtgttg attgccattt
     7261 ggacagccgt tgcactccgt acatctgctt cgatgcttcg agacttctga taaaggatct
     7321 gcataccgac aaccgtagct ccatattctg ctaagactag atcgtcatct gcaaacgatt
     7381 gttccatacg agataagata atcgtaccca aacgttcgcc agctccaaag atcggaacga
     7441 cagtcgtcag accaaatgga tatttctctc ttgactctac cggaaatgca gtaatatcac
     7501 tagtaattgg gatattagct tcagttttca tcaactgtgc cactgtatca gtataagatt
     7561 caggaaattg tttctcaaca aacattgttt tgattcgttc attgttcaca tcgtgacgtt
     7621 cattataacc tagtaatact ccgtcacttc ctatgatata ggtgttgctg tcaagaatac
     7681 cacctagtgt gacagccatt ttatcgtaag gcaaatctgc ttgcatgtca aaagtattct
     7741 tttgctgcaa aagtgtgttt atcttgcggg ttttcgttag caagtctgtc attgtcgtcc
     7801 tccgattttt tataaaatat agcgacttaa atcttcattt gccgcaattg agccaagttt
     7861 gtcatccaca tatgcttctg tgatcgttat ttctcccatt tgcatatctg gtgcttcaaa
     7921 taatagatct tctagtagtt tttccaaaat ggtatgcagt ctgcgtgcgc cgatattatc
     7981 tgtgtcacgg ttgacatcga aagcgatttg agcgatccgt tcaattgctt cttttgtaaa
     8041 cgtaactgac acattttctg taccgatcaa cgcgacgtat tgttttacta aagcattatt
     8101 tggttcggtc agaatcttca caaaatcttc agctgtcaaa tcatctaatt ctacccgtat
     8161 cgggaagcgt ccttgaagtt ctggaattag atcacttggt ttagacaaat ggaaagctcc
     8221 tgaagcaatg aataggacgt gatctgtttg gatgatcccg tattttgtgt ttacttgtga
     8281 accttcgaca attggcaata tgtctctttg gacgccttct ctggatactt ctccagattg
     8341 ctgtgattta gaagtgatct tatcgatttc atcgataaaa ataattccac tttcttccgc
     8401 taatttgatt gcctcactat ggatatcagc atccttgact aattttgctg attcttcctt
     8461 gatcaataac tctaaagctt ctttgactgt taccgtccgt tccacttttt tcttcggtgt
     8521 caaagcacct agagtttcat ttaggtcgat ccccatttgc tccaatccat tgttcatcat
     8581 tggtgcaggt ttcttttggt cttcaacttc aatggttact tcacgactat ccagtaaacc
     8641 cttttgtagt tgttccaaga ctgtacggcg attgattcga atatcttctg tcacttcttc
     8701 ttgcggttca gcggtatttt gctgactagc attaaaaaga ttcatcattt gttcaaattg
     8761 attagtggat tgtttctgtt cttttttgat accaggtacc aagattttta ccaaacgttt
     8821 ttctgcattt tttttagcac ttgtgtatac acggctgtac tgctcttttt ctacgatttg
     8881 gatcgcattt tctactaaat cccgaaccat tgattctaca tctcttccga cataaccgac
     8941 ttcagtaaat tttgttgctt cgactttgac aaatggtgca ttgacgattt ttgccaaacg
     9001 tcgtgcgatt tctgttttac cgacccctgt aggtccgatc atcagcatat tttttggtgt
     9061 aatatcttgt tgcatttttt catctaactg caaacggcga tagcggttac gcaaagcaac
     9121 ggcaacagat ttttttgctg cgtgctgtcc tacgatatat tcatctaatt cacttacgat
     9181 ttgtcttggt gtttgtttta attcatacat atctactcac ctcttacagt tcttctacga
     9241 taatattatg attagtaaag atacagatat ctgcggcaat atttaacgca ttttctgcaa
     9301 tttctttcgc actcatgtcg ttttgagcat agtttttcat tgctcgggct gcagccaacg
     9361 caaaattccc tcctgatccg atagctaaga taccatcatc tggtgtgata acttctcctg
     9421 tacctgaaac aagaagcatt tctttatcat tcattacgat cagcatcgct tcaagttttt
     9481 gcatagattg ctgtgtccgc cattcttgag ctaattcgac agctgcgcgt tgtagattcc
     9541 cgttatattc gtttagtttg ccttcaaatt tttcttctaa agtaaacgca tctgcaacac
     9601 ttccggcgaa acctacaaca acttctccat tgtagatacg tcgtactttc tttgcagtgc
     9661 ctttcatgac aacagattca cccattgtta cttggccatc tccagccata gcaaatttcc
     9721 cgtctttttc gacggcacag atagtggttg aatgaaatgg tgattcgacc atgtttgttt
     9781 cctcctaaat ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt
     9841 tttgtaaact ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc
     9901 ctagcaattc ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg
     9961 tatggcgcag catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat
    10021 tcaagacata ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga
    10081 tatcatgctc ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt
    10141 gcagagcatc ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat
    10201 ggatcaacat gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac
    10261 ttaaacggat gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag
    10321 gactatctcc tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg
    10381 gcagtctcaa atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt
    10441 cttctttgag aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat
    10501 ttctgctata gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat
    10561 attccgcctt cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa
    10621 tgtcttcttc atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac
    10681 gcaaaaattt ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag
    10741 aataatttag cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt
    10801 ctaagtttgt tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa
    10861 acgcaagaaa aagagcgaga ctatccgtct caccctcttc atggagtttc cttactcatt
    10921 tgggattgca gttttttcta attctgtttt tattgcctca ttagctttta aagctcgttg
    10981 tgcaattgct tcatatcttg cttttttgtc tcgaatgcgc tctggcagtt caggaaacag
    11041 accatagttg gcattcatcg gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc
    11101 catacttcca atagctgttt cttgtggaaa ctcgactggt tccatttctt ttgctaagcg
    11161 tgcggcgttg attccagcaa gtagtccgct cgctgcactt tcaacatagc cttctacacc
    11221 agtcatctga cctgcaaaga acagatcttc gcgtttcgtt gattggtaag tcggtttcag
    11281 caattctggc gaattcataa agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc
    11341 attttctaac ccaggaatca tccggaaaac acgtttttgc tcgccccatt tcaagtgagt
    11401 ctgaaagccc acgatgttgt ataaagaagc tgcagcattg tcttgacgca gctggataac
    11461 agcataagga cgtttgccag ttttaggatc ttccaatcca actggtttca taggtccgaa
    11521 aagcatcgtt ttgaatcctc gctgtgccat tacttcaatt ggcatacatc cttcaaaata
    11581 tttttctttc tcaaaagatt taagttctgc cacttcggca ttgattagtg cctcatggaa
    11641 agctttgaat tcctcttctg tcatcggaca gttcaagtaa gctgcttctc ctttgtcata
    11701 gcgggacttc aaatatactt tatccatatc gatcgtagct ttgtctacga ttggtgcagc
    11761 tgcatcatag aaataaaaac catctgaacc attgaatgct ttgatgcttt ctgctaatgg
    11821 atgagaagtc aaaggtcctg ttgctactac ggtgattcct tccggtattt cagtaatttc
    11881 ttcgtttatc actgttacta atggatgctc tttgactttt cgggtaattg tttcagaaaa
    11941 agaatcgcgg tcaacagcta aggcaccacc agctggcact gctgtttcat ctgccgaagt
    12001 gattactacc gaattcaatc ggcgcatttc ttctttcaat aaccctactg cattagccaa
    12061 actgtttcct cttaatgaat tggaacatac tagttcagca aaattacttg tgtgatgcgc
    12121 ctcagtcgat ttgactggac gcatttcata taaacggaca gggactcctg cttgtgcgat
    12181 ctgccaagca gcttcacttc cggcaagccc tgctccaaca acatttacca ttttcaatga
    12241 aagaacctct tttctattat ttttggatgt cttcttcgta atctccattt acacagacta
    12301 cttgtttacc gcctttgact ttcttctcga ccagatatcc gtcacatttt ggacaatttc
    12361 tgcccactgg tttatcccaa gaagtgaatt cacattctgg gaaacgggaa caaccataga
    12421 atagtctgtt tttcttagat tttcgttcaa tgacttgtcc ttcatgacat actgggcaag
    12481 tcacaccgat ttcttttacg attggtttcg tattccgaca atcaggaaaa ttactgcaag
    12541 cataaaactt cccatatttc cctaatttta tcaccatcgg atgtccacag acatcacaat
    12601 cgaatcctgc tggttcatct ttgatttgaa ttttttcgat tttttcttcc gcattcgtca
    12661 gttctttttc aaacggttta tagaaacgat caaccacttt gacccacgct tccgtgcctt
    12721 cttcaatttt atccaaatct ccttccattt ctgctgtgaa atggatatcg acgatttgag
    12781 ggaagaattc acagattaat gaattgacaa tttctcctag ttcagtcggc tcaaatcttt
    12841 tttgtgtcaa ttttacatag tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg
    12901 aaggtcgccc taccccgttt tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg
    12961 ctggtggttg agtaaaatgt tgttttggtt caatatcaac agattggact acatcgcctt
    13021 cttcaagttc tggaagaata ttttctttct cttctttgcc gtcatctcta ccttccacat
    13081 acacttgcat gaatcctttg aatttgattt ttgaaccgtt cgcaatgaag atgacaccat
    13141 tttgttctaa tgtcactttc attgtatcta aaatagctgg tgtcatctga ctagccacaa
    13201 aacgtgacca gatcaatgta taaagtttca attgatcttt atctaaatat tgtttgagtt
    13261 catctggtgt acgaagcaca ctcgttggac gcacagcttc atgggcatct tgtgcgcctt
    13321 gagcattttt gatctttcgg tgaccatgtc tagaaaattc ttttccatac gtatcttcaa
    13381 taaatgctgc ggcctctgct tttgctgtat cggcaatccg ggtagaatca gtacgcatgt
    13441 atgtaatcaa gccgactgtt ccttgtttac ccaaagtgat tccttcatat aattgctgtg
    13501 ctaccatcat ggtttttctt gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc
    13561 tacttgtagt gaaaggattc gcagggtttc ttttgcgctc tttcttttct acttttttaa
    13621 cttcgaagtc tgttccatca attctagcag tgacttcttt tacactttgt gcatctggta
    13681 atttcttttt cttaccgtcg attccccaga aatttgcttt gaatttcttg cgagcttttt
    13741 taaaattacc gtcgatgctc caatattctt ctggaacaaa ttctcggatc tctttttcac
    13801 ggtcaataat aatttttaaa gcgacagatt ggacacgtcc tgcacttaac cctttcttga
    13861 ccttacgcca gagaatagga ctgatcgaat aaccaacgat tctgtctaag gtacgacgag
    13921 cttgctgtgc atctactaaa tctacatcga tcgttcttgg ttccttaaaa gctgctttga
    13981 ctgcgtcttt tgtgatttca ttgaagacca cccgattttt ttctttcaga tccaacccaa
    14041 gtaggtacgc taaatgccaa gcaatcgctt ctccttctct atccgggtca cttgccaagt
    14101 aaactttttg agcttttttt gctgcggctt tcaggctttt gatgacatcg ccttttccgc
    14161 gtatagaaat ataatggggt tcataattgt tttcaaaatc gatacccatt ttacttttcg
    14221 gtaaatctct tatatggcca acactggcca ttactttata gtttcgtcca agatatttct
    14281 caattgtctt ggcttttgca ggtgattcta caatcactaa atatttatac gccattactg
    14341 aatggactcc tttccattgt cgtgttccta ctattaaaag aggattcata caaatcgtag
    14401 ctaatcatat ccattcagaa aacgtttgtc aagaaaagca aatgaaaagt atggtgttat
    14461 tctttaaatt ctgccaagat atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc
    14521 aaatgatgac atccggtaga atgttctgtt aaaatatttc ctggaacaga aaaaactgtt
    14581 cttccaagat ccaatgcttg ctgtgctgtt atcagagagc cgcttttttt cttggcttca
    14641 aagacacatg ttcctttaga caaagctgcg ataatccgat ttctcattgg aaaacaatgc
    14701 tttttcgggc cggattgagg aggatattca ctcaaaagaa gatgatcatt tgcaatcacc
    14761 tcttgcaagt cacgattttc agcaggatag acttgctcta tccctgttcc aatgactgcg
    14821 attgtccgtc caccatgaga gattgctgct tggtgaccgt atgtatctat ccctttcgcg
    14881 cagccactga cgatcacata tccagcttct atcactggtt ttagtaaata ataaactgtt
    14941 tttttggcta aagcagaagt gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt
    15001 aaagatagat caccattata aaacagtaca attggcggat aggtcaagtg aagcagctga
    15061 gggggatatt tgggatcaaa gcaagtgata aacgactgtt tcgattggat tttgccccag
    15121 ttagcgtgaa tttcctgcca acttttgcga aacagctccc ggtgtttggt tattttacct
    15181 atcttaatga tgtcttcaaa tccgatttct ttataaggat actgcatcat ccagttagca
    15241 agtgcccatt ttccttgact actgattcct tttgtataag caagtttcaa taaattttct
    15301 tctatttgat acatcataaa accgcctttc gtctctcttt ctaataagat acgtaaaagc
    15361 ggctgttttt tattgtaaat atttttttac aggtgcaaaa cttttacgat ggattggcgt
    15421 aattccttgt cgttcaagtc cttctaagtg ttcctttgtt ccataacctg cattatgcga
    15481 gaaaccatat ccaggataaa gcacgtcata ttctttcatc aattggtctc gatatacttt
    15541 agcaatgata cttgcagcag caatcgacac agatcgtgca tcccctttga ttattttttc
    15601 ctgctgaata ggcaaatcca aggtcatcgc atcgatcaac aaatagcttg gtacaatgga
    15661 caatttttct actgcttgga tcatcgctaa tttacttgct tcataaatat tcacatcatc
    15721 gattgtctgc tgatcaacga tcccgatccc gattgccagg gctttttctt gaacttgttg
    15781 ataaagaagt tctcgctttt tctctgataa ttgtttggag tcattcagcc ctaatatgat
    15841 ctcatttttc ggcaagataa ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc
    15901 tacttcatca atgccggcaa tccattggtg accttgtgcg tacccttgct tttcaaagca
    15961 aaacatttct tcatattttt ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga
    16021 agccagtgct tgctgcacgc ctttgcgtgg atcatttctt aatgattcta gtgtaggatc
    16081 atctattctc tgtatgcttt tcaatcgttc tttgatactt tggatggatt cagtcatcac
    16141 ttttttgctc catttcttcg caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa
    16201 tatcatgcac gactaattcg ctgccacgat catagtcttc tttataccca cgttttgctg
    16261 taatatccat cagtaattcc ggtgccaatt tttcttcttg ctcgattgat aaaccataac
    16321 gatcgctgat tcttcctgaa taatatcggg caaaaaagtc taatccgtaa atcgctaaat
    16381 catctaaatg gacaagctgg tctttgatcg cgcctgtcag cgctagtttt ttgccgattt
    16441 cttcatcttc aaatttgggc cataaaatcc ctggtgtgtc taacaattcc aactcattac
    16501 ctaaacgaag ccactgctga ccttttgtta cgccaggctt atttcctgtc tgtgcaattt
    16561 tcttgcctac tagtcgattc atcaacgtag atttcccaac atttggaata cctaagacca
    16621 ttgcccgaat agcgcgaggc ttgatccccc gagcagcatc tcttgcccgt ttttctttca
    16681 atgcttcttt agccgcaggt actagacctt taattccttt actttcttgt gccgtgatag
    16741 ctaatgcaag atggccttgt ttacgaaaat attcttgcca cagctttgtc tgttgtggat
    16801 ctgccagatc cgctttattc aataaaacaa ggcgaggttt ttgctgaatg atctgatcta
    16861 gcattggatt acgagaagat aaaggtaatc gtgcgtctac caattcaaat acgatgtcca
    16921 catatttcat tttttcagaa acttctcgtc gggcttttgc catatgtccc ggaaaccatt
    16981 ggatggtcat tgttctctcc attcctttca taccggaata ttccccggct ttacttgttt
    17041 ttttcttttt caatataaat tcactttttt att
//
LOCUS       DACESX010000050        16980 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.050, whole genome shotgun sequence.
ACCESSION   DACESX010000050 DACESX010000000
VERSION     DACESX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.050"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(346..960)
                     /locus_tag="IXF88_001428"
     CDS             complement(346..960)
                     /locus_tag="IXF88_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1508167.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1566..1943)
                     /locus_tag="IXF88_001429"
     CDS             complement(1566..1943)
                     /locus_tag="IXF88_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508168.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2243..3124)
                     /gene="galU"
                     /locus_tag="IXF88_001430"
     CDS             complement(2243..3124)
                     /gene="galU"
                     /locus_tag="IXF88_001430"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1508169.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3167..4186)
                     /locus_tag="IXF88_001431"
     CDS             complement(3167..4186)
                     /locus_tag="IXF88_001431"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1508170.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4819..5823)
                     /locus_tag="IXF88_001432"
     CDS             complement(4819..5823)
                     /locus_tag="IXF88_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1508171.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5836..6885)
                     /locus_tag="IXF88_001433"
     CDS             complement(5836..6885)
                     /locus_tag="IXF88_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1508172.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6902..7723)
                     /locus_tag="IXF88_001434"
     CDS             complement(6902..7723)
                     /locus_tag="IXF88_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1508173.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7710..8489)
                     /locus_tag="IXF88_001435"
     CDS             complement(7710..8489)
                     /locus_tag="IXF88_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1508174.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8506..8976)
                     /locus_tag="IXF88_001436"
     CDS             complement(8506..8976)
                     /locus_tag="IXF88_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1508175.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8988..9407)
                     /locus_tag="IXF88_001437"
     CDS             complement(8988..9407)
                     /locus_tag="IXF88_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1508176.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9488..12256)
                     /locus_tag="IXF88_001438"
     CDS             complement(9488..12256)
                     /locus_tag="IXF88_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1508177.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            12456..12680
                     /locus_tag="IXF88_001439"
     CDS             12456..12680
                     /locus_tag="IXF88_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508178.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            13459..15174
                     /locus_tag="IXF88_001440"
     CDS             13459..15174
                     /locus_tag="IXF88_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508179.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            15177..16940
                     /locus_tag="IXF88_001441"
     CDS             15177..16940
                     /locus_tag="IXF88_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508180.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         5015 a         3486 c         2889 g         5590 t
ORIGIN      
        1 tataattact ttgtaaagag ttttggggaa gactctttca tcaacgtgta ttaagagaca
       61 agaaagaatc catatggatt ctttcttgtc tctttttcca ctacttttta tttttctgac
      121 actacatatt cttataactc atttctcata actgtgaaac aacttataaa aataacaaac
      181 tttaaacaaa acaagcttaa aatcaaagat gatccatctt aaaataacta aaacaaaaaa
      241 agtagtcgtt gttaatcctc attaataatc gttttctttt tttcaaacgc tccattttta
      301 tgtggaacat tattttttta aggaactttg tcaacatgtg gataactatt ttccacttgt
      361 gtcctggact agctgttttg ctgtaaaata caaaacttgc tgcagcaaaa agatcgttgc
      421 aagagaggct gttccgcaga tcgctggatc tagatttggc aaagtcaaga ctgcatcatg
      481 taaattggcg tggttgtcca aaaccaaatc agcaaattca aataatcgca aaccactgct
      541 atgttgcgat ttgatacttc gtgacaaatc aaatccagta acaatgatta atggatgacc
      601 attttctttg atccactgag ctagttcgat gattgccggg tttcttcctt cattcgaaag
      661 aagaagaaaa atgtcttctg atttgacaga aagctgatca ataattgcct gtcctgcacc
      721 ttctagcgtt tcatagacac ctccggctgg attttcaatc aatatacttg gtacaagatc
      781 cggggtttga tcccaaaatt caaatgctac accacttagt gtgcggctag catatatctg
      841 caatctacca ccttgattaa tttgtttcgc gatccaagtg ccagcttgaa gcatcagcgg
      901 ctcttctatc cttaatttct cttccaattg gtgataatag tcaaaataac ataaactcat
      961 ttatctctcc cgcctagcta tacttgacat gaatagaaac agctgcctaa tctaaggaag
     1021 tacataaaaa aacagtagtt agacagtaac acatgtggtc tttgctgttt caaacacaaa
     1081 aaaggcattg aacgaatacg cccaatacct tgacatagca aataaaacaa cgtgcagacc
     1141 atggaaaata tctacatagg ggtagaattt tcaccctaga ttaggtacaa taaattaggt
     1201 cgatgcactg cattgactat ggcaaaatga tcggtcaggg tgtatgtgtg tgactggtca
     1261 ttccctgatt cttagcgcca actaagggtc agggagcctt tttttattca gctgtttcta
     1321 tctgaattta ttgtaagtaa tctcttatcc aagtgcaagc aagaaaatac aaaaaaacat
     1381 tggtatgatt tatatatata taaataacat aaaaaaagga atggcttatc ccgatacaac
     1441 aagcattcct ttaaaaaaga ttcttttata taagtgaaaa aagctttttt ccaccaatta
     1501 aataaatctc tcatctcaat agtttatcca ttattttatc tttttttgcg tatacctact
     1561 aaatattacc gtcttcttct cgttcttcta ctttgttcga gagaatctgt actagaaaaa
     1621 gtagttgtat gtctaccttt tctgcctatc cgagactttc ttttcatata tgggatataa
     1681 atgattagat acagtacagc aaaaaataaa gcaaatacgc aagcttccca actacctaac
     1741 gccaattcaa ccatcaaggt agcaacccca acggatgctg cataccagat aaaaatcaga
     1801 aacgtttctt tttttctgac catgcgaaac atgataaata gactaataag caatagtggc
     1861 aacacaaata tccaattgct tccttccatc agccacttga gtatatcaag cagcgactgt
     1921 gcaacagcga ctaattttcc catagtgaac acacaccttt ttttataatt aaaaaattcg
     1981 cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg taatcatttt
     2041 cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga atctattcac
     2101 gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc aaaaaactaa
     2161 aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag cctttcattc
     2221 tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc aactggataa
     2281 tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca atatttgtct
     2341 tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct ctcgcataga
     2401 ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg tttcctgctc
     2461 ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa cgtccgataa
     2521 tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct tttactctgc
     2581 aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat gtttcatttt
     2641 ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc atcatcaatt
     2701 gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg acaaacggtt
     2761 catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt ggatgagatt
     2821 gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt aattctgttt
     2881 tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga tcttcaatgg
     2941 gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc aatgcttctt
     3001 ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt gccatggctt
     3061 ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct tttttgacct
     3121 tcacttgctg ttcctcctaa acgacgatta actattgatc atctatttaa cgaagagaaa
     3181 attcattctc catttttccg tcacgtaaca tgatttcttt tgctgctttt ttgatatctt
     3241 ctccattata caaaacatta tagattgttt gagtaattgg catctcaaca cctagctgtt
     3301 gagctagctc tactgctgct tttgtcgttg ccacaccctc aacgaccatt cccatgtttt
     3361 ctagaacttc ttccagctta tgtccctgtc ctaacaagtt accggcacgc caattgcgag
     3421 aatgaacact tgtacaagta acgatcaagt cccccacacc actcaaaccg ataaatgtca
     3481 gaggattggc acccatcgcg acaccaagac ggctgatttc tgccaatcct cgagtcatga
     3541 ttgctgcttt tgcatcatct ccgaatccta atccatggat agcgccggca ccaattgcaa
     3601 tgatattttt cagtgctgca ccagtttcta cgccaatcac atcattattt gtataaatcc
     3661 tgaaataatc attcatgaac agtttttgta cataggctgc tgctttttga tcaatacttg
     3721 cagctgtaat tgttgtgata tcatgtactg ccacttcctc agcatggcta ggaccagata
     3781 atacaacaat tgcctgtctt ttttcttctg gaatttcttc agccaaaact tctgagattc
     3841 gtttatgcgt atcttgttct agtcctttac ttgcatgtat aatgattggc ttagtattca
     3901 ttttaccaat aagttcctgt gcgactgaac gaatcgcttt tgttggaaca acaaataaaa
     3961 tagcatctgc gttatccact gcttctgcca attctttata tccgataatc gattctggga
     4021 tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt attgatttca tcaatctgtt
     4081 caggtacatt tccccaaata cggacatcat gcccgttttc tgccaatgtt tgtgctaaag
     4141 cagtgcccca agatccagga ccaagaactg cgattttttg tttcatggat attcctccaa
     4201 ataaataatt gataatttcc attatagtat acatttctta aagttgggac taatttccat
     4261 tgtttttaag tgtttgttta cttcttttat cgtttttact tgaaataatc aatgatatgt
     4321 tacaatagtc tacattttat ttttacactc gttatgcttt gcttgttcct aagaggaagc
     4381 agttttgaca atgttactac cagcattact attaggaggt taggaaactt ctcctagtga
     4441 gtattgattt aaaatacata agagacagat tgaacatttc taaatctgtc aggtcctgca
     4501 acggacagat tgtctctgtt gacggttgac ataggaactt ttgttttagt caacattctg
     4561 gatacaaaac gctatgtaaa ctggcgcaac tcgttgtact tggactttta ctacggatcc
     4621 atgcaacaac taaatagtac tattgcataa caaaaaatgg ttggcaccca catgtcccat
     4681 taaacagatt ataagttgaa ctgtagataa tgcaaaaaat gtgttttata acgtaattga
     4741 tgccaatatt ttaaaaaaat aaagggagca gttatcgctg ctctctttat tttttatatt
     4801 ctttttatcc agtctatcct aataatcaaa ctgacgatag tagcggcgta tctctaatgg
     4861 atggcagttc aactgctcca catgagcatc gatgcgttgt gtcactacat gcatcaataa
     4921 gaaagacaaa acacgtccgc gatatttttc actgatacct tctactggat agtcttttgt
     4981 gtctatcaat gtataattgc tgcaaatttt tggcaaaagt ttagccacac gttcagataa
     5041 cactctctgt tcatcttcgc ctaaaaataa tgtgactggg gttgtttctt ctacgatctc
     5101 taacgtcccg tgaagaaact cggcagcatg gatagatttc gatggaagcc agctttgttc
     5161 ttcccagtaa cacatggcat atgaataaac agctccccat tgattccctg ctccaataaa
     5221 ataatgcata ctatcacgtc gatgtttttc tgcaaatgat aagccaaatg catctgcttt
     5281 tttctctgct tccactaacc ctgttggtaa ataacgttcg agctgttcat agtattctga
     5341 ataagcttcg aattctccat tcaaatacat caatcgatct gctgccataa aaaatttgat
     5401 ttgttctgtt ccaggagctg gataagtgac cacaaaatcg catagctgag acaacttact
     5461 gttcgcttta tcgataaatc caatcagtgt cgctcctact ttcttgattt gttctactgc
     5521 tttcactact tcttgagttg taccggtaac agaggacaaa ataacgatag aatcttttgt
     5581 taatcttttg tttccagtag tgtaatattc tgcagcatgc tgtacaaaga caggcaagtt
     5641 gcttttccca ttcatataag tgacagcttg catcgaggaa gcatacgttc caccgattcc
     5701 tagataatag acagcgtcaa atttttccgc ataaagttta tcgatgattt cttctacttg
     5761 cggacgtaaa gctagtgcgc cttttattgc tttgatttgt tcttcttcat tgaatttcag
     5821 catgatttcc acctcttata atttactttt caagactgta tcaaagtctt caaaaatctt
     5881 tttgttcaag tcaatacctt tagctactgc ggttttataa gcgaatagtt gaaacggaac
     5941 gacaagcgct aatacagaca gatattcatc acattctatt gctaatccaa gtgttttctc
     6001 gtttcttgct ttttttgtgg taatggtgag agtctctcca acgtaccgac tcatatatct
     6061 ttgcaacttt tgcgaacgtt ccttatttac actttcccct tcaataaaga atagcaaatg
     6121 cgaagcatct gcctccaggt atgggccgtg catataagct tctaactcaa atccttgtga
     6181 cggaacgcgg attgtttcag tcaatttcgt ctctgcttct ttagctgttc cccagtttgg
     6241 accatatcca atggtgacga aacgctgtgc taaacgaaat aaactttgat atccatcaaa
     6301 aaattcttcc gttttttgaa taatcgctgg taaatggttg attattttgc gcaactgttg
     6361 catgtagtct tgttctattt ttttgctgat tttattttta cttattccaa ttcctatccc
     6421 taagagtagt aactgtaaaa cagtcgcaga atagcctttt gtaacgaagc ctactgattc
     6481 taccccggca cctaaatcaa taatttgtgc agcttctaaa gcaagtggac tttgcacgtc
     6541 atttgtgata gcaacaacag gtaacccttg tttttgtatc atttttactg cttcaacagt
     6601 agaagcactt ttcccgcttt gtgacacagc aatcacagta tcgattgaag gagaaagttt
     6661 tccataattg aaaaaataaa aaggttcttg aatatcgaca gtaagatcac cataagattc
     6721 caatgctggc ttcgctgcta aacaagcgtt ataagatgaa cccgttgcta agatcagtaa
     6781 attcactgtt tttttcatat gttctcttgt cgaaaaatca ttctttttca agatcgtact
     6841 tagtacttcc ttttcttcat agatataatc ttgcatcgta aacattttct ctgctccttt
     6901 tttaaaaaat ccctatccac gaaccaaaaa ttccaaagaa agctagtccg atcaagatcg
     6961 ttaatggttt cacttctttc ttcaatagat aaaacactat gccaaatgct ccaagacata
     7021 agatatttgg aacgatatca tcaaaaatac tttgaacagt cacagcattt tctcctgaac
     7081 cgatttttaa aggaatattg atggtgatca tcgtggcagt cattgctcca acgaccatca
     7141 gtccaataat agacgcgcct aatgtcaaac tttccatcat accgtttgcc tggatctttt
     7201 tcaaaaaacc cgttcctaag cgatatcccc aacctgttgc tagatagcga aacagaatgt
     7261 gcggaatatt gaaaatcaaa atgaacaaga tagggcctaa aatatttcct tgcatggcta
     7321 aagaggtacc cacacctgtg gcaatcaatc gcaaagttcc ccaaaagaaa gaatctccta
     7381 accctgcaag aggtcccatc aaagatgttt tgatactatc gatcgcagtc acatcaaatt
     7441 caggatcatc cttattttct tcctccattg cagccgtgat tcctaaaatc agtgtaacga
     7501 tatgtggggt cgtgttaaaa aactctagat gccgtttcaa agctgctgcc atttcttctt
     7561 tcgactgata taactttttc aaaatcggaa tcattgcata aacataagcc atattcatct
     7621 gacgttcata attccaagaa aactccattt gaaaagaacg ccaaaatacg tgatttagct
     7681 cttttttcgt tattttgctg tttttgatct cagaattctt catcttcttc tacctcccct
     7741 gcagttgttt gaagtggtag ttgacggaca ttcataatat tgactaccac aactgctaaa
     7801 atcgcaccaa aaatagctac tccggtcaca ggaatctcta agtaagaagc tatagcaaaa
     7861 cctaagaaaa aatatggtgc aacttgtttg ttgatcagca aacgagcaag catcgcaaaa
     7921 ccaagagctg gaatcaaccc tgtagctact gacaacccct tttgaacgaa gtcaggaatc
     7981 atatttaata gatttccgat cgtattgctt cccaccatat aagaaaccat taccacaaca
     8041 ccaagcatca aggaaagacc gataccggca agcaagtgca tatgctcgac tcctttataa
     8101 tttccttctt ctgcataatt gtctgcttta tggttcataa ttggaatcaa aagccctaag
     8161 taaatatttt tcaatatcaa tgtcaacgta gcaatcggca aacctagtaa aagcgcggtt
     8221 tctgtaccgg ctccagctgt aatagcaaaa gccgtcccta aaatcccacc ggtcacaaca
     8281 tcaggtggaa tcgaagcacc tactgagaac gatccgataa atgctaactc taaagttgcg
     8341 cccataatga tcccagtttt tacatcacct aacacgattc ctgtaaacag ccccgtcaca
     8401 atcggacgag aaagcaacga tgtccctagt gcatattctg attgtgcgac aaatgcaaca
     8461 attcctaata agactgcttg tacaatcatt gaggttcttc ctttcttaca aaacttctct
     8521 tacatccact tttttatctg ctggtacttg acggatttct acttctattt gtttgtcaat
     8581 taatcgtttc aagcgttcct cctcttctgg taaaatattt accgcttttc cgatgctgcg
     8641 agtaccaggt tttgccttga tccctcctaa gttgatggtt ttgatttctg gatacgcttc
     8701 tgccagctta tatgcatcct cgatcgattc cactacgatg aataacttat atttatctgt
     8761 caccccgctt ttcaacgcgg caatcgagtc atcaattgat ttgatcacta gtttgactcc
     8821 ttgtggcttg gctaatttga tcgttgtctt tcgtatctca ttcgtaggta catcatcatt
     8881 agcaatcaaa atacagtctg cgcctattgt ttgtgtccat gaaaaagcta cttgtccgtg
     8941 taataaccga tggtcgactc ttgtcaatac aatcattttt cttcctccta aaattcgtct
     9001 tcttctattt tctgattgag ccgctcgtta caaaactgga tcattttttt tgaatcagtt
     9061 agcacctgct ggatcaaaac agttgtctct acttcagcgt ccaaccgaga tactagctca
     9121 atcaatagtg gcaaattcaa tccggcaacg agatagacat gatcccgatt gatatattgt
     9181 aagaattcgt tgttgacact tccgccaaaa agatccgtca gaacaatgat ttctttatct
     9241 ttgtacctca taaaaagctg ccttactgtt tccgctaaat caaaattctc atcagtatag
     9301 caatcgaata cagtaataga atgatttttc ccatagatca gctctaatga atctaatatc
     9361 cctgaagcaa attttccatg ggaagctaag ataatttctc tatccacttt ttcatctcct
     9421 cttttcactt acacatacta aaaaagcaag ttgcatgcca aaattagtgg caacaacttg
     9481 cttatgttca aaattcttct tctatcgaga gaaactctgt atcttcactc aaaatatcat
     9541 agatataggc aatctcagaa ttggggatca tgacactata atgctcttca atgacactaa
     9601 acgcagattt aatagcagat aacccttctt tttgacactg cttccatttc tctaatcctt
     9661 gataggtttc gattggcatt tgtcggatca atcgttcaat gagacagctc acatggacat
     9721 acaacgctaa ttttttggga ttgctgatgc ttcgattcaa ctgcacctct aattctctca
     9781 taaaatgatc gatttctccc atcaccttct ctgtatctag aatagtcacg gaatccagta
     9841 ctttttccaa tgaaaaatta cgaatgatgc gctgattgaa tatttccttc tctgaagcat
     9901 tcatagtagt gtccaaccat tcagctagta cagacatctt ttcccccgag atgaggtctt
     9961 ctaatgaaag atagtcgata ttggtgatat caggatctgt ggtgccaatc attcctacga
    10021 cttcgtatat ggaaaataat gggtcactgt ttttcttatc ttttaattgc tgatattcat
    10081 atgggatgat tttcaactga caagccgtag gtaagctttt ttctagcagt tcacttaagt
    10141 ggaccgccgt accgattcca gtgaagcaag tagtaatgag tgctttgggt ttgttttcct
    10201 ctggatagag cagtcgccat tctggctgga tctgtcgaac agtctcttct gctatctctt
    10261 ctaatgatac atttttttgc aggcattccc ctactgtgat tgctaaaggt gtcgtcacat
    10321 tattcaaaat caaaagcggt acggataact gttttttaaa aaactgatgg atttctttca
    10381 gagatcccat gtctactaat atgatcaatc catttgaaac atcgctgttt tcgcaataat
    10441 caaggatttc tgaagcaatc tgctgtggtg tgacatctag tggcatatca aatgattcaa
    10501 atagatcctc ttgtaataaa cgatttacta catttgccat actgctagca gttgcataac
    10561 cgtgagcagc aatcatcgct tttggcagtc gtttttcttt tgaccatttc gcttttttta
    10621 gatagatcgt cagtaaaatc cgatccatac ttgtgtcttc aatatctaat ttgatcttac
    10681 ttaattccag aattcgttgg acatagtgat aactagatgg ataagactga gaaacttgtt
    10741 gttctaattc tttgattagt tggataatat catgttcttc cggtaaccat ttgatagtgc
    10801 ttcgctgata gagatagtaa ctcaaggcat agacactgtt tccatcgaat ttgatttgat
    10861 aggcagtttc catttgtcga aaggtctctc ggatagattg tgtccaataa accagcagct
    10921 cgtgtttttc ttgtctactg gtctcaaaca gcaaaaaatc aaataattga atcacttctt
    10981 gctttaattt tccttcgcaa gaatatagca gacagttatt tttcttgaaa tcaattagga
    11041 tttgctcaaa acttttaacg attcgctgct gttcgggctg ttgcttctcc attaattgct
    11101 ctaaggtctg gtcttgcgta atatagattc cttgctgtgc tgtattttta gtaataggct
    11161 gcgcaagtag ttctttttgc aaatgatgga tcgtgacatg gatttcaggc tgttcacgct
    11221 gttcagcata tgcttttgct gctgaaactt tgacgatatt tttcagctct cctatgtttc
    11281 cggataattt ctgacttgtt aaaagtgaca aagctggtcc actgactgac aaagggctcc
    11341 ccatttttct ttgttcttcg agaaaaaaag agtaaatcag ttctagccgt tcattacgac
    11401 tacgctgatt aagtgcaggt agttcgattc ggataggtat tcttcgaatg aaagttgtta
    11461 agaaatgact agtaatctct tcagtcgtag caaaaaagag acgtgttttg atccggcgtg
    11521 acaaattagg ttctcccata cgataaatca caccttgatc aagataggtg aacagttttt
    11581 cctgaccttc tggtgttaag cgatgtacct cgtctaaaaa caagatccca ccatctgctg
    11641 cttcaaaagc ccctttttta ttttcttctg caccagtgta cgctccctta acatgtccaa
    11701 acaaattgct agtcaaaagt tcaggattat ctgcatattg tgcacaatta aaagtgatga
    11761 atggtgcaga atcttctagc agatcatgaa gcagacaata ctgatagacc aaattaacta
    11821 aataactttt acctgtgcca ctttcgcctg taatcaaaag tggtaatccg ccatctggat
    11881 agtaaagagc tgttttgatt tgttcgatac tttctttcaa actggcgtcg tatccgatca
    11941 taaaagagaa taagtctttt ggttgcgcga aaaaaggctg ttcctctttg agatcatcga
    12001 tagaaaaata aaaattcgtg cgtaacttaa aaaattgttt ttcaaaagct tctttgtgga
    12061 aataaatcac tggtcgagaa ttgattttga ctagtagttg ctcatcattt aactggttca
    12121 aataatgact gactgtgttt ctttttaccc caaaatgatc agctaacctt tgtgctgtaa
    12181 agacctcgtt catatgttct aaatcgaaaa aggctgtctg gttctctaag taacataaaa
    12241 tttccttctt caacaactta ttcacccctc ctccatcata gaaaacggat tcattttgtc
    12301 aataaaatct ttaataaaga attgaaaaat atatcttatt atagacagca gcctacttat
    12361 cccttataat ttttattcta aaaaacgtac agttccttcc agtttttata ctggaatttc
    12421 aacatatgaa agaataagga ggctatgact attcaatgaa atcggataaa tataaaaaca
    12481 ggaaacatac ttcctggaaa acttttcgag aaatcatcgg atcttatctc gctgtagttg
    12541 tatttttgcc tttgctttat atggctatga cagaacaaac tcgttttatt ggcgtagata
    12601 cgcaatatat agccgaaaca ggatttttat tcatctttat cttgattttt gcgttctata
    12661 aatccttcca tacaaaataa gattcacaga tttcaacttt cctgtctgtt tttagctatt
    12721 acaaatcctt atatgtggta taacaacaat ttattggcat aatgaggaaa aacgagttat
    12781 gatgctttca gagagtaaag aggtactgtt gtcaccatcc tttttattga ttacgtacac
    12841 cactcttagt tttttcccat tgattacgaa aacaaggtag aaacattgat gttcctacct
    12901 tgttttcttt ttttattttc agatcaaaat ctcaagtagc ttttgacttg atgtcgggag
    12961 gttgtcttcg ttccatcaaa cctgatagaa tctgcgccaa accatagttt tctccactat
    13021 tatttattcc tctaagatag tacccacaat aaacgaataa atatttttct cattttcata
    13081 ctccttcttt gaaaatcaat tagatttctc tcaacccact tttttacaaa aattacgtag
    13141 caaaaaagat tgcaagaaca aattaaaaaa gaagtaaata gagtttcatc tataaacatt
    13201 tacttgatat attttttatg cagaattcat ttatagaaaa attaagtaaa atagacgttc
    13261 ctatgaatga agagactggc atgttttctc attttctttt ttttaaaaaa aatagtctat
    13321 tcaatttcta aagcttcttc ataataaaac tttcatttat gaaaaggctt gttctctttt
    13381 cattctaaat ttcctaagct acaatgtggt agtacttgtt ttttcaattg tggacaaata
    13441 aataatagga ggaatcatat gattcagtta ttacgttatg caaaagacta tcgtaagcaa
    13501 atcattttgg gcccgttctt caaatttctt gaagctgtct tcgaacttgt cctgccttta
    13561 atgatggctt ccttgatcga taatggacta aaaatgaatg accgaggaaa aatcattgaa
    13621 atgggcttat ggatggtcgc tatgtctgtg attggtctga tttgtgctat catttgccaa
    13681 tactatgctt cagtagcttc acaaggattt ggtacagagt tacgtaatca gctaatcaaa
    13741 aaaatcaata ctttctctca caacgagttg aatcattttg gaacagatac cttgatcacc
    13801 cgaatgacaa acgacatcaa tcagcttcag ctagctttag caatggtcat tcgtttattg
    13861 atcagagcac cgtttttaag tatcgggtca gtggtcatgg cttttgtgat cgactgggaa
    13921 gtaggtctgt ttttcttagc attattgcct attttctcaa tcattttatt ttttatcatc
    13981 aaaaaaaccg ttcctctcta ccaaaaagtg caagaaaaac tagatcagct caatgaacac
    14041 gttagtcaga atcttagcgg tgttcgtgtc atccgcgcat ttgcgcagac aaaaaaagaa
    14101 acagagaagt tcgataaaag cacaaatgat ctagcgaaca attatttacg tgtttccaat
    14161 atctctgccc tattatcacc tgctacaacc ttgatcatga atgtcgggat catctgtttg
    14221 ctgacagttg gcgggatcaa agtaaatatt ggtagcttgc aacaaggaca agttcttgct
    14281 ttgatcaatt atatgaatca aatgcttttg gcactgatcg ttgtttcaaa cctcgtcgtg
    14341 atcttcacac gtgccgaagc ttcaggaaat cgggtgaaag aggtgctaga cacagaaaat
    14401 tcgattcttg aagctgaggt cagtactact cccgattttt cttctgaagc aattatcgct
    14461 tttgaccatg ttgacttccg ttatactcct gattctggtc tttctctcca aaatattaca
    14521 tttcagctga agcgaaatac agtccttgga atcattggtc caaccggaag cgggaaaacg
    14581 actttgacac aattgattcc gcgtttctat gatgtcagtt ccggtacagt tataatggac
    14641 ggaatggatg ttcgttcttg gcagctggat catctccgtc agcaaattgc tcttgttccc
    14701 caaacttctg tactatttac cgggactatt cgtgaaaacc ttcaatgggg taagaaagac
    14761 gccacagaaa aagaatgttg ggaagctctg aaaatcgctc aggcagaaga atttgtccgt
    14821 cagcttccaa atggtttaga tacaaaagtc atggaaaatg ggaaaaattt ctctggcgga
    14881 caaaaacagc gactgacgat tgcaagagct ttgattgcca agccagcatt actgatttta
    14941 gatgactctt taagtgcgtt ggattatcaa acagacttgg atttgcgtaa ggcgctacag
    15001 acttatcttg gcagtacagt gattattatc tcccaacgtg tccgctctat tcaagaagca
    15061 catcatatct tagtgatgga tcaaggtaag attgctgctc agggtaccca tgaagaatta
    15121 cttgctcaat cagcagaata tcaggaaatc gtcgcatcac aggaggaaga atagttatga
    15181 aagtaaaaaa attatccttg cagacgctcc gccgattcat tccttacctt actgcctatg
    15241 caaaagacat ttggctagct gtgatactag gaatcatcaa tgggattgca acagttgtga
    15301 tgacgttaca aattggtcaa agtatcgatc aaatgattgg agaaggacag gtccgcttca
    15361 atcgactgtt ccatttactt attctattcg gtggtatcgt tttaatcaac gtagtcagtc
    15421 aatggctcat ccaagtgtta agcaatcggt tagcttactt atccgttgca aaattaagaa
    15481 aagatgcatt tgctcatctg aatcgattgc cacttcgtta ttatgatcaa aactcccacg
    15541 gaaatatcgt cagtcgattt acaaatgata tggacaatat ttccattgca atttctgcgg
    15601 ccttcaatca attattttct ggagtggcaa tcatcattct ttcgtttatt tttatgatgc
    15661 gtctaagtcc acttctgaca ttggtcgttc tttgttctac accaattatt ttccttgtga
    15721 gttggatcgt tgctcgtgct tcacaaacaa atttcgatag acagcaaagg attattgggg
    15781 atatctcggg ctttgtgacc gaacgaatcg gtaaccagaa aatcgtgaaa gcttttcagc
    15841 aagaagaagc tagtcaacag caatttgaac tgctaaatgc tgatctatat gaaaaaggac
    15901 aacgggcaca attttcttct tccttgacaa acccgtcttc tcgttttgta gatcaccttg
    15961 cttatttatc gattggttta gttggcggcc tattggcatt gaagagtggt tcgtcaatca
    16021 ctgttggtgt gatttccagc tttacgatct actcaagtca gttttctaaa ccatttatcg
    16081 aactttctgg aatcacgaca caactgcaaa ccgctttttc tggtctagaa cgagcatttg
    16141 atttgatcga tcagccagaa gaaaagcctg atttgccaga tgcctatgcc ttggaaccca
    16201 aaaatatcaa aggaaaagtc tcttttgatc atgtttcttt ttcttatgac cccaagcagc
    16261 gtttgatcga agatttcaat tttactgctt atccaggtca aaccattgct atagtaggaa
    16321 aaacaggggc cggaaaatcc acacttgtta atttgctgat gcgtttttat gacacaacaa
    16381 aaggaacaat tacagtcgat gaccatgaca tccgctctat ccaacgtgat acattacgta
    16441 aaagttttgg aatggtgtta caagatactt ggctatttga cagtacattg cgagaaaatc
    16501 ttcgttttgg gaatccggaa gcaagtgacg cagaaatcaa tgatgcccta aaagcctctt
    16561 atatgtatga atttgtagaa cgtttgccgg aaaaattaga tactaagatt ggcggtcaag
    16621 ggttaaagat atcagatgga caacgccaac tgttgacgat tgctcgaacg atgatcagta
    16681 atccccctat gttgatctta gatgaagcta ccagctcagt cgatacattg acagagaaaa
    16741 aaatccaaca agccttctta gcaatgatgg aaggaaaaac cagttttgtg attgcccatc
    16801 gcctgtccac aatcaaaaat gcggaccaga tattagtgat ggatcaggga gctattgtcg
    16861 aaatgggcac ccatgatgag ctgcttcaaa aagatggtta ttaccgacgg ctctatcaag
    16921 cacaattcac agcacaataa aaacaagcag tcttctggga tgggaagact gcttgttttt
//
LOCUS       DACESX010000051        16960 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.051, whole genome shotgun sequence.
ACCESSION   DACESX010000051 DACESX010000000
VERSION     DACESX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.051"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(470..3175)
                     /locus_tag="IXF88_001442"
     CDS             complement(470..3175)
                     /locus_tag="IXF88_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1508181.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3481..4428)
                     /locus_tag="IXF88_001443"
     CDS             complement(3481..4428)
                     /locus_tag="IXF88_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1508182.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4618..4887
                     /gene="rpsN"
                     /locus_tag="IXF88_001444"
     CDS             4618..4887
                     /gene="rpsN"
                     /locus_tag="IXF88_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1508183.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(5063..5425)
                     /locus_tag="IXF88_001445"
     CDS             complement(5063..5425)
                     /locus_tag="IXF88_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1508184.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5436..6557)
                     /locus_tag="IXF88_001446"
     CDS             complement(5436..6557)
                     /locus_tag="IXF88_001446"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1508185.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6573..6923)
                     /locus_tag="IXF88_001447"
     CDS             complement(6573..6923)
                     /locus_tag="IXF88_001447"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1508186.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(7053..8564)
                     /locus_tag="IXF88_001448"
     CDS             complement(7053..8564)
                     /locus_tag="IXF88_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1508187.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8886..10238)
                     /locus_tag="IXF88_001449"
     CDS             complement(8886..10238)
                     /locus_tag="IXF88_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1508188.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10391..11467)
                     /locus_tag="IXF88_001450"
     CDS             complement(10391..11467)
                     /locus_tag="IXF88_001450"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1508189.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11663..13408
                     /locus_tag="IXF88_001451"
     CDS             11663..13408
                     /locus_tag="IXF88_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1508190.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13688..14125)
                     /locus_tag="IXF88_001452"
     CDS             complement(13688..14125)
                     /locus_tag="IXF88_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1508191.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(14109..15482)
                     /locus_tag="IXF88_001453"
     CDS             complement(14109..15482)
                     /locus_tag="IXF88_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508192.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15804..16292)
                     /locus_tag="IXF88_001454"
     CDS             complement(15804..16292)
                     /locus_tag="IXF88_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1508193.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(16314..16916)
                     /locus_tag="IXF88_001455"
     CDS             complement(16314..16916)
                     /locus_tag="IXF88_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1508194.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4770 a         3625 c         3078 g         5487 t
ORIGIN      
        1 cagaaaaata tcaagcgctt tttcaaacgc tttaatacag caaaaatcta acattttttt
       61 atgggctttt tctttgaatt ctataataaa aggatagaac ttgacttaga aaatcaagtt
      121 ctatcctttt tgatatctta acttttggaa gagagatgta aggctgcttt ctacccatca
      181 ttcttactac tcgaagcata gcgcagttgt cacaacctct tgataaacgg tgtttaaaag
      241 cttgcatctg cggtaataag cccaaccaaa ttaaaaacat aagaagctgt ttttatttgg
      301 tcgttcttat tacttgatgc agagcagctt ttttacaacc tcttggtaca cggtgttcaa
      361 tcagctgacc ctcggtatta gctcaaaaaa acggcaaaat atgaggaact atttttccat
      421 ttttttctct aatactcagg tcaaaacact gatttcacaa cctctttttt taatgaaaaa
      481 tatggctctc atgcgccgct tctttgaatt tcgtcatttt ttcttcgatg aacggataga
      541 ttgccgttcc tacgattcca aagacaacaa acaaaccgat catgatccag attgctttcg
      601 tagcatttgg ccaataaatt cctcctgcag attcccgtaa caagtttacc gcatgagtaa
      661 atggcaagaa cggattgatc atttggaaga atttccctga gacttggatc ggatagttcc
      721 cgcctccgcc agagatcgac agtactaaga tgataatagc gatcccttta ccgacattat
      781 cgaataatgc agccaagaca tagacgatca tcatgaacgc taaggcgatc aagacagcaa
      841 atgacacgct gtagaccgga ttttggacat ctactcctag taagaaaata ttccctaatg
      901 taacgatcaa gctttgcgca atcgccattg tcaaaaatgt cagcatacga gcaacgaatt
      961 gttctcgttt cgtaaaattg tttcggtctt tctttcctaa atagtattct gtcgtagcaa
     1021 cacttgaaag aagaacagcg ccgacccaca gacatagtgc agtataaaac ggggtgctag
     1081 ctgaaccgtt atttgcgatt ggataaagcg cattggtttg tagctcaaca ggttgtgtaa
     1141 agaagtcact ttcttttgtt gcgtctagtt tcaataattt gatgacttcg cctagatcga
     1201 cttcttgttc gccttttcgg atcgcgtctg ctgctttttg gataccagct ttgatatttg
     1261 gccagtcatt gttgatcaag tccgcagcag cgttcacagc tgtttcgact tctggcattt
     1321 tttcatttac tgttgtcaat gtaccggtga tttcattttt tagtgctggc caatcatttt
     1381 ggataaaatc agcggccaat cctaattttt gctgaatgat cggtaattca ttgttgtata
     1441 ggtctgcccc ttcattgatg ccattgacga tggtatccat atttccattc agcattgtat
     1501 tggcatcatg gatttcttgt ccaattgcag gcatttcttt ttgatatttt tctaaaattg
     1561 ccactgcatt cgttacagtt gctttcgttg agctgagaag cgaatcaaag tcgatctggt
     1621 ccgctttgct cagtgcgcct tgtgcagtcc caatcgtatc gatgacttgg tctagtagag
     1681 aagtgacagt ggaactgatt tgacctgtat tgataccagc aatgatgcca ttgatattat
     1741 taacaacggc atcaatttga tctagcaatt catcaatagc cttcgtgtct tcattttgaa
     1801 cagctgtgtt caaagcagat aggcggtttt gcagattgct tagcaagggt tttaattgat
     1861 ttagctgatc aataattggc tgtaatttcg tatttccagc acttgtttgc aatttagtca
     1921 ggaactcgac cagtttatcg attgcatttt gttgttgaga tattccgtca ataaagccgt
     1981 tcaatacatt gctgatgttt ttcttttcat catcagtcag cttaccatct tcaatagctg
     2041 ttcttacagt atctattaac gaacctgtcg ttgttgccac ttgtccaatt gacttcaatg
     2101 taacatctac actgtttgtg atgcttggta tcgcatcttt cagctgagag gcacctgatt
     2161 ttgtttggtt tgctagatca cttgcctgat tttctaaatt tttcacatct ggcaagatat
     2221 cttgtacttg ttggatgatc gtcaagcctt gttttgcttc atcgattcct tggttcattg
     2281 tctcttggat cgaagcaaaa tcgccgtcga tctctgctag ttgcttaccg gcattttgga
     2341 tctctgggat tttttcttgc aaatcaagga tgatttttgc ttgatctttc aactcaggca
     2401 ttttgtcatt caatgcaacg actttttcac ccatctcatc tactttaggg atgtaatcca
     2461 cgaattcatt tgctttattc agtttttcct tgatttcagg tagttgactt tgtaattcga
     2521 ggacttgttt tgtgtagccg tcgatcgtgt ccaaattttc atctgttgat aaaatcatat
     2581 ctttaacttt attgatactt actaggttcg tatcgatgtc gtaacctatc tcattgaata
     2641 ctttcaataa agtcgaactg gctgttttga taaattcatt tgtgatttgc gattggatcg
     2701 aacttgctcc tttatcggtg attttcggtg caatcgcatt gattttttca tttaccgtat
     2761 attcgatttt tggtttcttg atatcaccac tagtaaaact cagcagatct tctgagaaat
     2821 cctttggcaa gtaaatacct gcgtaatact ttccggaacg gactccgtct tctaattgtt
     2881 cttttgaatc aacgaattgc catcctaatt gtttattttt atgaagcgat tcgatgactt
     2941 gctccccaat ggccacttct ttaccttgaa actctgctgg cttgtctgca ctgtaaacag
     3001 caattggcag ctctccagta tttccatatg gatcccataa tgctttgatg ttaaaccacg
     3061 catatagcga agggatgatc atgatcgcga tgatcagaag tgttgcaatc gggtttttga
     3121 aaatccgttt ccagtcaagc aaatataatc gaagcgtatt tttgattgac tgcatagttc
     3181 tcccccttct tcttttttca gctgaattac tttaccatgt atttcatact tttttcaatc
     3241 ggcttttttc aaaaaagtcg aatttttgga catatcacgg caattgtctc attgtaatct
     3301 cttacatatt gagaaataat taaggattac agcctttact ttatcatgaa ataaaaggga
     3361 aagataattt tatgccaaag tttttatcta ttatacgtat acgcaaaaaa gctaaaacat
     3421 tttttgtctt tgcccctaca aaaatgtttc agcttttttc gttttttatt cctgtatttc
     3481 ttatttttcg aatgacagga tgattttccc agcaggagga tgtccttcaa ctagctgatg
     3541 tgcttggatg acatgcttaa gtgaagcaga cagattcatt gaaataaaaa catgaaaggc
     3601 tcctttttgg taggctttga ttatgccagc aaaggcttct gcatctagat attcctttct
     3661 tggaacaaac gattcataaa agccttcctt tttttgccgc aaatctagat ctgggaggtc
     3721 atttaatgca acatacctgc cgcctggttt catgatctgg atgccggttt ctcctttgat
     3781 acttcctttc gtcgcatcga ttacaaggtc tgcctgatca gcaaattgca gacctggatt
     3841 tgttttatca taagcagcaa atgctgaagc tcctaatttt tttacctttt cctcattttt
     3901 actggatgca ctggttaaga ttcggattcc cttttcatgg agaagctgga tcagacttga
     3961 cccgaccgca cctgatgctc ctagtatcat tacagtatct gtcggctgga tttccgtcaa
     4021 atggttgatt agattataag ccgtaattcc tggcgtgacc attccggctg cttcttccca
     4081 actcatttta tctggaattt ttgctacttt tgctgatggc aacacgactt cttctccgta
     4141 agtcccgccg actgcatgga cagctactcg atcacctaca tgaacagttg tgacatctga
     4201 accaatctct gtcacaatac cagcgccatc attacctggt acataaggga atttcaatgt
     4261 tcgtatctct ttcatttctc ctaaacgcaa ggcaacatca taaggattca cactgaatgc
     4321 ttttatttct acacgaacat gtccagaacc gacttctctg ggatgagcat cgatttcttc
     4381 aaaaacatct tgtgcttctc cgtaaccgtt aattgcaaat cttcgcatgg tcaccatctc
     4441 cctttttctt taagtttacg tttatgcctt tattttgaca aaaaaaatgt cttttcgttt
     4501 tggcgcatga ttcaaaacaa aatagttgtc ttttttcgcg taacgtgtta aattatttat
     4561 ctaaaagcgt aaagtttacg atttacgcta ataaaaaata attggaggaa tttgttgatg
     4621 gctaaaaaat caaagattgc caaagcaaaa aaacagcaag aaacgattga acgctatgct
     4681 gctttacgcc aggaattaaa agcgcaaaaa gattacgaag gcttgcgtaa acttccttta
     4741 gatgctcgtc cagaacgatt gaaaaatcgt gatttaatcg atggacgccc tcgcggatac
     4801 atgcgtaaat tcgggatgtc ccgtataaaa ttccgtgaat tagcacataa aggtttgatt
     4861 ccaggagtaa aaaaagccag ctggtaatcc aagtctggct gtaatgtaaa ggaggagggc
     4921 gcatggacca ctccttggat catcaaaaaa gatacgttcg cttcttctag cagaaatgct
     4981 ggtactagaa gcagacgtat ctttttttac gcacagctgg tcctttttta tatgtaaagg
     5041 ctgcatggtt acttcgctat atttatttag taacaggagt cagtgcttcg atacccacac
     5101 taattttcaa tgctcggtca acttctgcca taatgggtcg atccaaatgg cagacctttt
     5161 ctttcaatct tgatttatcg atcgttctga tctgctccaa caggataacg gagtctcttt
     5221 ctattcctgt tccagtagct ttgatcccaa tgtgagtcgg aagcttaggt ttagccattt
     5281 ttgccgttat ggccgcaaca atgatcgtcg ggctaaaatg gttacctaga ttattttgaa
     5341 taaccaaaac tgggcgtgtt ccgccttgct ccgatcccac gactggagaa agatcagcaa
     5401 aataaatatc tcctcttttt accataggat cctccttatt cttgatattc tcttggtatc
     5461 cgctgtccta atccgcaagc cacttcatag tggatcgtac ctaattgatc tgctatatcc
     5521 tgcatagtga tttctttgtc tgcatttttg ccgataagcg tcacttttgt tccgacagga
     5581 aattcttgag gcaatcggat catcagttga tccatacata cacgcccaat cgtttcacag
     5641 tagttccctt ctaccaatag agaaaaaccc tgcatctttc gtggccagcc atctgcataa
     5701 ccgatcggta tcgttccgat ccactctgct tcaggagtga tatatgtttc tccatagccg
     5761 attccttcac ctgctgggag ttttttgact tgaatcaatt cagaaaccag ctcaagtgct
     5821 ggctgcaatg gataaacttc cggtagtgcg tgcccagagg gattaagtcc gtacattgct
     5881 actccataac gaatcatatt gccgattgtt tcatcatgcc ataaagccgt tgcactattg
     5941 ctgacatgta catatcgagg aagttcggga agttttttca gcacttctat aaagcgttct
     6001 ttttgcagat tccaatatgt atcatctgcc tgatctgctg tagaaaaatg ggtaaataaa
     6061 ccttcccatt ctaatacacg actttctttg atgaatgcag ctgcctcttt tacttcttca
     6121 ggtgtacaaa atccgatacg acccatccca gtgtctgctt ttagatgaat cgaaagaggc
     6181 gtctctagct tacttttctc taaaacagca gctgctgctt gcaaccattc ccgtgtacct
     6241 gcagttactg ataaatcatg agcaacagct acagtcagtg cgtcgtaagg aaccatattc
     6301 aagataagga tgggctgagt aaatccagct tctctcaact cgactccttc atctaaattt
     6361 gaaacacaaa agcccgaagc tccacctgca actgctgcct cagctgtttc aatagctcca
     6421 tgaccgtatc cgttcgcttt tactactgca aacaattctt ttccttgcgg aagtcttgca
     6481 acttcatttg ctacattttc agttatagct tttttatgta tcactgcttt cgttgggcgg
     6541 tgccaagcga caaccatcgt ttttccttct ttctagtctt ccaaaataat ttgtgcaaaa
     6601 gctgtttcat ttgtatgagt aatactgacc catacattgc cttcatgagg tgaacgcgtc
     6661 acaacaggct gaccattttc attttttaaa atctcgacat cttgaaaggt aacttttccg
     6721 atcccagttc cccaagcttt agagaaagcc tctttgcaag cgtaacgccc gcctaaaaat
     6781 tctacttggc gatgaaaagg caaggactgg aatagtttca tttcatcaga ggtcaagatt
     6841 cgtgcaatga attttggttt ttcttcgatc agccgagtga tcctaggaag ttcgactgca
     6901 tcgatcccaa ttccttttat catatgcttt tctcttttca ataaatttag aacactctca
     6961 ttctaccaaa ttataaccat tttgccacaa ataagtccac aaataacaaa aatgaaagtt
     7021 tctcttaaaa aagagaaact ttcattttat gtttaatcgt tatttgaacg gattacaaat
     7081 ccgcgttttt tatcattgtg acgtttaccg ccatcttttt ggctgttatt atttttgcta
     7141 taaccgcctt ttgatttgtt tccacgataa ttaccgccgt cacgacgatt acggttgttt
     7201 ccgccaccac gtttgccatt tttattgtat ccttttttct gcattggcaa tgggcgttct
     7261 ggtgtgatct tcactggcac tgcatcagct ggatcttttg ctgtcgtttt tagcaaaagt
     7321 gcaactaaat cttgtgcaga atattcttct aataatttat ctgctgtttg caggtattta
     7381 tctagaccat tttctgcaag ttttgtttcg atttgttcaa ctgcagcacc taattgacct
     7441 ttgaatgctt ctttttcggt tggtggacgt aaagtcgtca tccgtttttt cgtcaagttt
     7501 tcaatcacat gcaagtagtc catttcattt ggtgtaacga aagtcacaga cataccgcct
     7561 tttccggcac ggcctgtacg tccgatacgg tgaacgtagc tttctggatc ttgagggata
     7621 tcatagttgt acacatgagt gacacctgaa atatctaatc cacgtgcagc aacgtctgtc
     7681 gctacaagga tatctaaatg accgctctta aatgaacgca atacgctcat tcgtttttgt
     7741 tgagacaaat cgccatggat tccttctgca cggtaaccgc gtgcttctaa tccgcgtgcc
     7801 aattcgtcta cacggcgttt tgtacgtccg aagacgattg ttaattcagg tgtttgaaca
     7861 tcaaacaagc gagtcatgat atcgaatttt tcgtattctt ttgcgcggac atagtattga
     7921 tcgatcaagt cagctgtcat ttctttcgct ttgatcttca catgatgtgg atttttcatg
     7981 aatttaacgc caatattctt gatagctggc ggcatcgttg ctgagaaaag taatgtttgt
     8041 ctttgatcag gaacttgtga aatgattttt tcgatatctt ccaagaagcc catattcaac
     8101 atttcatctg cttcatccaa aactaatgtt tgaacggttc caagtttcaa tgtgtggcgg
     8161 ttaatgtggt caagcatacg tcctggtgta cccacaacga tatgtggacg atctttcaag
     8221 ccacggatct gacggccaat atctgctccg ccgtaaacgg cttgtacacg tacttttttg
     8281 tcgcgcccta aacgatataa ttcttcttgt gtttggatag ctaattcacg tgttggtgcg
     8341 ataactaaac cttgtaattc gtgacgatct ggatcgattt tttctaacat tggtaatcca
     8401 aatgcagctg ttttccctgt acctgtctgt gcttgtccaa taacgtcttt tccagctaat
     8461 gctagtggaa tcgtttctgc ttggataggc gttgcttctt caaaaccagc gcgttcgact
     8521 gatttcaaca attctggtga taattctagt tctttaaatt tcaaatgaat cctcctatat
     8581 ggttgttaac tgaggatgta gcttttttgc taccccgtcg atttgttcgg tcaaagtgta
     8641 ttgtttcttt ttgacagtgg ccacgcacgt ttttatccat tcttagcccc tgtggcacca
     8701 ccgaaaaatc actttgcgaa gtttaggcat tagaaaagcc aaacgggtcg cgttcagctt
     8761 tatcattatt atgacatctg gcttcaacaa gttgtttcta tggcaaaacc agtccaattt
     8821 ctcttcatct ttcggcaata ggttattcta acatagtttt tcgttttctg caagtttaca
     8881 gatttttatt tcatttgctg aagttgttct accacttcaa ataagcccat gctgttgctg
     8941 ccttttagca taatactatc tgttgcttca agattttgtt tgatagcatc aatcaattta
     9001 ttcttttctt tttccaaata gaacacaggt agttgggggt atttttggtt caattcttct
     9061 tttagtgcat gcatttgttc cccataaagg aaaatcatgg cgaatttctc aggatcgata
     9121 tgttcactca tttgcgcatg catatttaaa gactctttcc ctaattccag catatctgct
     9181 aacacagcga ttctgcgtcc ttttgttggc agatttttaa aagtatctaa taccagtccc
     9241 atggctgttg gatttgcatt gtagacatcg ctcaaaatag ctgcaccatt tcctgccgtt
     9301 agccattcgg ttctgttttg agtgatagaa acatgggcga gcccttggcg tatctcaggt
     9361 atggacagtc caaagaagcg cccgatcgta tacgcaatca aagcattcgt tacattataa
     9421 cttccaggca gcgggatcgt aaatgtttct ccttctacta caaaatcggt ttgtgctttt
     9481 ccttttgctg tcacttccgc atgaacatcg ccatttccaa taccgaatgt tgtgactgtt
     9541 tgtactaatg ttgccgttaa aggtgtcagc aacggttcat ctgcgggcac aacgagtaat
     9601 ccgtctgata caagtccgtc agtgatttcc attttggctt tagcgattcc ttctcgcgac
     9661 ccaagatttt ctatgtgtgc ttcaccgatc attgtgattg ctgcagcttt tggttgagcc
     9721 ataagtgaaa gttccgtgat ttcattggcg tgatccatgc ccatttccaa aacgatcttt
     9781 tctgtatctt ggggcatgtg caaaatcgtg taaggaagac caatattatt gttgtaattt
     9841 ccttgcgtct tatatgtttt gtatttttca gacaagacag acgcggttag atctttcgtg
     9901 gtggttttac cattgcttcc tgtaattgca acgacttctg cgcctacttt ttccaagtaa
     9961 taggttgcca attgctgcat ggctttcaaa ggatctgcta caggaataac tggaaaattt
    10021 tttggagctt gttcaacagg caagctccaa aatgaagcag atgctccgtt tgtgatcgct
    10081 tgctggataa attcatgtcc atctcgcgta ccagcaagag gaacgaataa acttccttcg
    10141 gttatcttcc ggctgtcaaa ttcgacattg gtaatctttg tcagtgcaga gcattcgctg
    10201 cccacggctt gtgcaatttc ttgtattgtt agattcattg agatctctcc taaaaaaggt
    10261 tgtgaaaaaa gcgtttggcc taaacggcgg gttcgcatga ctctcacttc caagtaaatt
    10321 tctctattcg tatttagaag ctagaatgaa cgcggttacg caatcactcc agcctcttct
    10381 ttatttctct ttattcattt ttttcaaaaa aagattgacg ctgatggtat cgattcattc
    10441 ctaactggat cagttcttca atcaaatcac cgtatttcaa gcccatattt tcccataaga
    10501 gtgggtacat actgaactcc gtaaatcctg gcatagagtt taattcattc aggaataatt
    10561 catttttatt tgtcaaaaag aaatcgcacc ggctcaatcc gcttccacct aacatcgtgt
    10621 aagctaactt cgcgtactct tgcgcttttt gataaacttc ttctggcact tcggctggaa
    10681 tctgcatttc gattttatta ttgatatatt ttgcttcata atcatagaat gctacgtctt
    10741 ttacgacttc accaggcaaa gtcgtccgaa cgtcttcatt tcctaataca gcaacttcga
    10801 tttcgcgcgc ttcaattcct tgttcaacga ttgctcgaga atcatactga taggctgttg
    10861 ctaaagcatt ttgcagctct tctcggtttt ctgcttttgt aatgccgaca ctagaaccca
    10921 tattcgccgg tttgacaaac atcggataaa gcaaagaacc ttcacattga tcaaatactt
    10981 ttttaggatt ttctttccat tgattcttaa gtactggtac ataaggaact tgcggcacac
    11041 cagcagcttg taaaatatac ttggtcatga ttttatccat ggcacatgca ctggtcaata
    11101 cgcctgcgcc gacataaggc atattcaatg tctctaagaa gccttggatc gttccatctt
    11161 ccccgtttgg cccatgtaaa actggaaaaa cgatggctcc ttcttctttg atttcgcccg
    11221 gattgatcac ttttcctgta aagccttcct ctgtctgtcc acttgggtcc catgaaagat
    11281 gcaagacatc tttgctggca ggtttttctg ttagtaatgg acctttgacc cattgtcctt
    11341 cttttgtaat aaatacgagt tgaacttggt aataattata ataaatggca tttaaaactg
    11401 aaaatgcgga aagaatggac acttcatgct ctgcgctgcg tccgccatat agtaaagtaa
    11461 tcttcaaaag attgtcctcc tgtattctaa atcgttcagt gatttactct catattttag
    11521 cacaaaacaa aaataaacga ataacttatt tataaaaaaa cgagccagct tctaaaagaa
    11581 cgagcgtcat ttattttttg tcagctagat tatttgtccc gttttttata aaaagttatg
    11641 ataaagaaaa ggagggatga tgatggcgca aacaaatcca cgcagccgct tggcagaaat
    11701 cattcgagtg ttcatcaatt acaatgtagt gccaaacttt gtacagcaaa aaaatcccga
    11761 acaggtaaaa aaagcctttg aagaactagg ccccaccttc atcaagatag gacagatgct
    11821 ttctgttcga gaagatttgc tttcttctgc cttcactcaa acattcaaaa cattacaaga
    11881 tagtgtacct agcgacacat tttcaacggt caaaaagacg atcgaaacag aactttctct
    11941 ttcattatca gatatattcg atgatttttc aaaatcgcct tttgcctctg cttctatggg
    12001 gcaggctcat cgtgccaagc tgaagaacgg tgacgcggta gtcgttaaga tccaacaccc
    12061 taacatcgcc gaagagatcc gacttgatct tcaactattt gaacgagcga tccctttgat
    12121 caaatacatt cctgaaacta gcgtagttga tctaaaaggg gtactccaag aagtaaaacg
    12181 ttccttgatc aatgaaatgg acttcttaaa agaaagtcaa aatggcgaac aattctatca
    12241 aaagaacaat ggctggaaag agatccgcag ccccaagatt tatgacgctt tttgttcgaa
    12301 aaaagtgatc gtgatggaag aaatgtctgg taaaaacttg aatcatttaa tgaacgccga
    12361 aaacaaaact gaaactttta tcactggaat tcaaaacaag cagctgaaac aagaagttgc
    12421 aaagctttta gttgagaact ttatgaaaca ggtgtttgat gatggttttt tccatgctga
    12481 tcctcatcca gggaatctgc tattccatgt actaacaaaa gaagaacaga cacaagctag
    12541 ccgaaaaact gaaacagtcc atgaaaaaga gttcggttcc tttgcttttc gagcctccac
    12601 ttctgcagaa gaccctgttg cgccatatac catcaactac atcgattttg gtatgatggg
    12661 gcatctttct gcaggactta ggcaaaaact cacacaagcc gttttagcgc tgtatacgaa
    12721 agatgcttat cgaatcgaaa aagcggtcct tcgactttgt cagcaagaag gctcgtttga
    12781 tgaaagtcgg ttccaccaag aactgacaag ttttcttgaa cagtattatg attctcctat
    12841 tgatgaaatc aacttgcaag aagtattcac acatgtcgtg acgatttgtc atcaaaataa
    12901 tctgcagttc gatcgtgata tcacgctttt gctgaaagca tttgggacat tagaaggggt
    12961 gatccgcgtc ctcgatcctg aagtttccct catggaagtc gcttcgcctt ttgctcaaca
    13021 ttattttctc actcatctgg atgtagaaga tacattgaaa caaagcggat tagacttatt
    13081 ggaagggatg aaggctgcgc ctaagatccc tcaacagctc caccatctat tagaaatgtg
    13141 gacttcggga caaggaaaag tcaatttgga attgaaaaag caagacaaat tattgagcag
    13201 gatcgaaagt atgatcaatc ggcttgtttt cggtatgatt ttagctgcat tgatcgtagg
    13261 gtcctccctg cttgtccaag cagcacctgt agaaaacgca gaggtcgttt ccttattagg
    13321 gatatttacg tatgctattg ccgcattcgt cattattttt ttggcaatcg atgcattgat
    13381 ccaaatgtat aagaagagga aaaagtagaa aaaggttgtg acagaaagaa tcagcttcaa
    13441 gaaataagaa ggggttttac ataaattgtt tctcaaattt ttgtgaaaat tcggcttatt
    13501 tttcgaagag gctacttctg aaacaccgag aataaaaaat gtgaaaaagc tgttctgcac
    13561 ccagtaataa gaacaatcaa aaagggaaca tggaacacat ttatcctgtt tccctgttcc
    13621 ctttttgatg tattcgagca taaataaaat accttcgtta cctactatct tctgtacttg
    13681 gtaaacctta gtcaattgcc ttttttaacg cttgacgaaa agacatatga tctttctccg
    13741 taaagggttt tggacccttt gtccttatgc cagcttttct taactttcca ctatgataac
    13801 gttgttctag aagcgtgccg atattcgctt cgtcaaattc ttttccagag tgatggaaca
    13861 ctcgcacacc cttcggcaat acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt
    13921 cattcttctt aaccataccg acgattttat aatccgcact atcagctccg cggtctacgt
    13981 agacgatttg gacatgatta gcgtattctt tggtcgtata atggtccaca ctggtcacga
    14041 taaccacgtc aagaccatag gattctccta attttatgac atcttctttt actggagaac
    14101 catctccatc aatgaatagc ctcattcttt ttcgccttct tccataaatg ataaacagtt
    14161 actaatccgc tcagtaaaag accaacattg atccattttg ccaagataag cagttgtaca
    14221 ctttctatcg gcactgattc atggttcagc gtgatcaata atagatggat tggctctccc
    14281 atttcttttg ctaaaacaaa caaaagcgtc atcaataaaa actggtggat cacactccct
    14341 ttattttgag tagtatactt gacaacgatc cggcgatcca ttggaatagc tgtatcagtt
    14401 tgataatatt ccttatttag ccagctgcct gttactacgt gccagaaact aagcagaaag
    14461 acacctatcg caaaaccatt tggaatcaag aggaccaata aagaagaact cagtccaagc
    14521 aattgcatag ccaatggaga ccatgttaaa cgtttcaaaa tccatggtcc gactattttt
    14581 gctgcaatca agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg
    14641 tacagtatgc caacataaaa actgctaaat aaaaaaagga aattccctag cattccattt
    14701 agaaaagtaa acagatttag ccagcttggc aacttccatt ccttcttctg gtgtcctaaa
    14761 acaaacacga acaagatgaa cagcagcaag aaaccataaa tagcaagatc aaaaagcctt
    14821 tcatcgaata acaatcgggc atttctcaaa agaaacaata agccaaagaa tatcaaaaat
    14881 aaaacaagct ctcgcgtagc taccagattt cttttgatgt ctttgaaatc cagttcgtaa
    14941 cgcggataat gactaaccgt atggtaggcc atgacgtaaa aaagtgtata aatagtaaag
    15001 cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag aaatagccag
    15061 atagctgcaa ataagtactt tttcccatcc atattgataa aaccttgcgt cttttcgtga
    15121 aaattgaccg tggtattagc aggtacaagc catgctgcac tcagccctag caaaacactt
    15181 gaaaaataaa ataaaggaaa ataaaatacg cctaatattc caagcaatga accagcacca
    15241 ccaatcagca gagcgatcat cagcaacgtg taactatcta gaccaaatcg tagactgttg
    15301 actaaaaata gtccagtcat ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc
    15361 caattatgcg agtctgttag tcccaaaaat aaaagataag gaataaaaat aaccgtgtta
    15421 cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac
    15481 atgacaatgg ctccttaaac agtcttataa ctaatctgct cttcgatttt agcattaaag
    15541 gggaaataaa ccaattttac agaatatatt cttgtaaaaa aaaatacaga aagaagcata
    15601 agcttctttc tgcattttta cggtacttga ttactcaagc agacaagccc ttctccacaa
    15661 aagtcttgcc cgacacccct gcatccggca ggtgataaag atagaaatca attatcctgt
    15721 gcctccaaaa tcaattagga gctctgtaaa ccagagtcct tctcgacccg tcgcataaat
    15781 ttactttaac acagttccta tgtttatgcg agagcttttt tcattttata atcgaccatt
    15841 tgaaatcctt gaccgatatc tgaaataagt tcttctgtaa tctcaaatcc ttcatgctta
    15901 taaacctcga ttgcttgatg attcccctga ttcactcgca aaaagatctc tctttccttt
    15961 gtttgcgcag caagttcttc ataccagcga aaaactgaag aagtcaatcc ttgaccacgt
    16021 actttattta gcaaatataa tttacttaaa taaatttttc catttatctc ttcataagca
    16081 gtgtaaccga cagtcttttc ttcgcttttt aataaaaagt aggacacacc ttctgtcagt
    16141 tctttttgaa tctgtttttt tgactgataa gttttcagca tgtaatccac ttgtttttga
    16201 ccaatgatcg gcacaaaagc ttccggccat acttcttgta tgactgccat cagctcatcc
    16261 agttcatttt ctgttacttt tattttttcc atacacccac gtcctttttt acattataaa
    16321 tgaccttgcg cttgatggaa taaaaagagc atgatcatgc tatacacacc caccgaccat
    16381 ggtttcaaga aaatcacaat ctgcataaat tccctaaaag ataatttcgt caaaccagct
    16441 actagacaga tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac tagtactttt
    16501 ttaatatctt tcgtttttct tgtcagcaat tcatcaaatt tctgatactt ttttggcgat
    16561 aaaataagct ggacaaaggc tacgccatag taacggacaa gcaaaaatcc gattgcttcc
    16621 ccaatcacta ggccaataca actatataaa agcccaaatc cattgccaaa catcagcatg
    16681 cccgctactg aggagatccc tcccggcaaa ataggaatga tcacttggat cacttgcaac
    16741 agaacaaata tcatcactgc atacttgcca aattgctgga taaacgcttg caaagacgtg
    16801 ctcgatcgaa aaatcccatg atgataagca taaagaatca acacaaagaa aaaaaacaga
    16861 ccaataatgg gcatgatctg aagacatttt tgtaaaactg atcctttttt agacatctat
    16921 attccccaca tcttttttca tttattaaaa ctgcctcctt
//
LOCUS       DACESX010000052        16878 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.052, whole genome shotgun sequence.
ACCESSION   DACESX010000052 DACESX010000000
VERSION     DACESX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.052"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(258..1763)
                     /locus_tag="IXF88_001456"
     CDS             complement(258..1763)
                     /locus_tag="IXF88_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1508195.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1760..2446)
                     /locus_tag="IXF88_001457"
     CDS             complement(1760..2446)
                     /locus_tag="IXF88_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1508196.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="IXF88_001458"
     CDS             complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="IXF88_001458"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1508197.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="IXF88_001459"
     CDS             complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="IXF88_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1508198.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4467..5036)
                     /locus_tag="IXF88_001460"
     CDS             complement(4467..5036)
                     /locus_tag="IXF88_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1508199.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5222..6124
                     /locus_tag="IXF88_001461"
     CDS             5222..6124
                     /locus_tag="IXF88_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1508200.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="IXF88_001462"
     CDS             complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="IXF88_001462"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1508201.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="IXF88_001463"
     CDS             complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="IXF88_001463"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1508202.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="IXF88_001464"
     CDS             complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="IXF88_001464"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1508203.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12692..12877
                     /locus_tag="IXF88_001465"
     CDS             12692..12877
                     /locus_tag="IXF88_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1508204.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12939..14135)
                     /locus_tag="IXF88_001466"
     CDS             complement(12939..14135)
                     /locus_tag="IXF88_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1508205.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14305..15741)
                     /locus_tag="IXF88_001467"
     CDS             complement(14305..15741)
                     /locus_tag="IXF88_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1508206.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16034..16843
                     /locus_tag="IXF88_001468"
     CDS             16034..16843
                     /locus_tag="IXF88_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1508207.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4952 a         3539 c         2950 g         5437 t
ORIGIN      
        1 aaataataat atagcaatct ctttataaaa ttgcaataga aaattcccta tttttattta
       61 ttatttttaa acacgaatat tacagcattt tattgttttt tcgttcatct aaaagatact
      121 tacatttaat taacggatgt tattctattt aattgttcgc tagttttttt ataatgacat
      181 ccctgaattt ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa
      241 caagtaaagc acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa
      301 attgtccctt gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt
      361 tgtttagcga tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc
      421 actcgataaa aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag
      481 tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt
      541 cgtgtagttg aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga
      601 ttacgataaa tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag
      661 tctggatata atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt
      721 gtttcattcg cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga
      781 actaagcttt tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc
      841 tcaggatcat ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca
      901 ggtgtcctta actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa
      961 cgcatacggt caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg
     1021 tcgatttcag gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct
     1081 ctgagtacct tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt
     1141 aagatcaaag aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca
     1201 tagaaagaag ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta
     1261 gaatatatag gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga
     1321 actttacgtg tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc
     1381 tcgtccaaat cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggaa
     1441 tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca
     1501 tttgtcgctt tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta
     1561 gccaaacgag aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca
     1621 atcagattga tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg
     1681 aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt
     1741 tccgattttt tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga
     1801 tatagctttc ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca
     1861 ctacgtttgt ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg
     1921 ctaataccac gttcacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta
     1981 gttcaattac ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc
     2041 gataagtaat agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca
     2101 acaatgcacg aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat
     2161 ctgctccgtg gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa
     2221 tgattggtgt atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg
     2281 ggagcatcag atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag
     2341 ttcggccgtt gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa
     2401 atcgtgccaa gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc
     2461 tttctattct actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc
     2521 tatagtcatt ttattatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat
     2581 cgacttcagc tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt
     2641 attcttcatg gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat
     2701 gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt
     2761 atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag
     2821 ccaaggcaac gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat
     2881 catctagcaa taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca
     2941 agaatcgagc tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca
     3001 agtcccaatc aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca
     3061 tgattttact gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg
     3121 tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat
     3181 aagcagaaat atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt
     3241 caagcgcgct ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca
     3301 catctactac tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga
     3361 tttctatcaa atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt
     3421 cttctactga aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca
     3481 tatctccata ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc
     3541 caatatatgt gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg
     3601 gtgcaactag cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag
     3661 ctccttcttc accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact
     3721 cttcattacg acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc
     3781 ctttatcaag gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga
     3841 ccattagcaa gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg
     3901 ttgctttgaa atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac
     3961 gattgaataa agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca
     4021 taacggccat accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg
     4081 ggatttctcc ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac
     4141 aacatatttt ttatttaccg actacaaaaa aataaacctc aaggctccat ggttagcctt
     4201 gaggttttac attttctaat taaaatgtat taagcttctt ttgacattac gtccttacca
     4261 tcatagtaac cacaatttgc acatacgtga tggctttttt tcatttcacc acagtttgga
     4321 cattcgttta accctttgat tgtcaatttg tagtgtgtac gacgttttgc ttttttagct
     4381 tttgatgttc ttctagctgg tactgccatt ttgctacacc tccttgtaaa gtcttgtatc
     4441 taaattagac acaatatatt ccaatcttac ttgttatcgt cgtcttttga tttagaatca
     4501 aaaagttcag atagttttgc aagacgagga tcaattttat tctcggcttc ttccattttt
     4561 ctgcgctggt attcttcttc ggaaacaact tcccaatcat tgccttttgg aagttctgtt
     4621 gctgcttcct cttcttttgt caatacccgc attggaatcg ccaataagat gttgtcttca
     4681 atcgactctg ctaaattcaa taatggacct tcaattagca aaatctcatc tgcagacatt
     4741 tgttcatctc tactttggaa ttgttcttct gtcatgaata cttcgtctac agaaaaatcc
     4801 atgggcagtt caacaggttc tagtgaacgt gttgaaggaa ctgtcaaagt cgtctgcaca
     4861 gtgtaatgaa cgatatagcc tttcttatca actgagacca aaccttcgac ttccactggc
     4921 gcaacatcaa gtattaaatt gtctcgcttc atgagagcgg ctttcaagtc gagcgtctca
     4981 tgaaaagtta acggtgtctc ctggtatttt ctcaattcca ataaagacca tttcattttt
     5041 catcactcct aagcaacaaa agcaattata cagggcaaaa agcatgttgt caatgaaatt
     5101 ttcttgacac cttgcagttc ataaaaaaac agaaaaatct ttattagaaa gcctctgttt
     5161 gctatcatcc tcagaatcat ttacaataat cattattcca ttaaaggagc ggatgatctt
     5221 catgaacacg aaagatatcg aatactatgt ccaaatcgtc aaacaaaaaa attttacgaa
     5281 agtggctaat tatttccgtg tgagccaacc tactattaca tatgcattac aacgtcttga
     5341 aaaagagttt gacaccgtac ttattacaag agatcgatcg catcacgaat tgatcgtcac
     5401 gccttctgga aagcagctgt ttctccatgc acaaaccatt ctccaagaga tttctactgc
     5461 aagagctgaa atcactcagt tgcaaaagaa aaagcttcgt tttggcatgc ctcctattat
     5521 cggcaatgaa tactttccta aactgtcaag ttactttatc aagcatgaat tgatgaatca
     5581 aattgagatt accgatggag gctctcggga cttatacggg atgatccgac aagggaggat
     5641 cgatttagct gtattaggct caacacagcc aatcgtggat caatatttag aaacagagtt
     5701 acttttggac aaacgtttca tgatcgtcgt gccacctaca catcctcttt ccaagcagga
     5761 tactgtctct ttcaaagata tagcagatga acagtttgta ctattgaacg aacattatgt
     5821 ccaccccgca gcattcacta agctttctaa acaaacacat actcatccaa aagttgttta
     5881 tcaaagcaat gacttgacga ttttaaagag tatggtccga gaaaatgtgg gtatcggttt
     5941 tttggcagag attgctgtcc atcctgaaga tcatttagtt tgcctccctc tttcagatga
     6001 agttcagcct cgcttcttga tttcattggc aaaacgaaca cagcagatcg tcacgcctct
     6061 tcaagaacaa gtagtccaag ttatccatga attcgttaca gcacaaaaaa acagctcttc
     6121 gtaaaagaag agctgttttt attttttatt aagctaaatt agatagctgt tgtttcgcca
     6181 cggtaaacga tgccgcgacg tgaatcaacg gtaatcaatt caccttctgt aattgttgtt
     6241 gttgcgcctt tagcaccaac gattacagga atatcttgtg caattcctac aactgcagcg
     6301 tgagaagtca atccgccttc ttcaacgata acagcagctg ctttttcgat tgcaggcata
     6361 tattctttgt cagttgatgg tacaactaga accattccgt cttttgcttt ttttacagct
     6421 tcttcagcag atgaagcaat aactgcattt gcaacgacag atttttgtcc aacgccttga
     6481 gcttctaata atttagaacc aatcatttga attttcatca cgttagttgt tccgcgttca
     6541 ccaactggta caccagcagt gatcaagatt aaatcgcctt ctgatgcgaa acctaattca
     6601 acagcttttt tagctgctag ttcaaacatt tcatcagttg ttgatggttt ttcagcaact
     6661 gttgggtaaa cgccccagtt taacatcaaa ccacgttttg tgcgttcgtc aaaagtaaca
     6721 gccaagatat ctgcatctgg acgatatttt gaaatcattt ttgcagtgta tcctgattca
     6781 gttgcagcaa cgattgtttt cacgcctaga ttttttgcag cacgagctac agataaaccg
     6841 attgtttcag ttacatctgt tttatcgaat tcgttcaatt ggaatgtacc taattctgat
     6901 aatgcatttt cagcttcttg gtcgatacgt gccattgttg aaacagcttc aactgggtag
     6961 tccccgtttg cagattcacc agatagcatt gttgcatctg tgccatcaaa tacagcgtta
     7021 gcaacgtcag aagcttccgc acgtgttgga cgtgggtttt cttgcattga ttctaacatt
     7081 tgtgtagcag tgataactgg gataccagca gcattacatt ttttgataat gcgtttttga
     7141 accattggta ctagttcagc tgggatttca acacccatat ctccgcgggc aatcataaca
     7201 ccgtcagaaa ctttgatgat ttcgtcaatg ttgtcgatac cttcttgaga ttcgatttta
     7261 gggaagattt gaacatgagt catgtctttt tcttctaaga tttcgcggat atctagaacg
     7321 tcttgtgctt tacgtacaaa acttgcagcg ataaagtcga tatcgttgtc taaaccaaaa
     7381 cgaatatcgt cagcatcttt tggagtaata cctggcaaac taatagaaac gcctggtgca
     7441 tttacccctt tacgtgatcc tagcatacct gcatttttaa cttctacgac taattcacgg
     7501 tttgcttcgt ctttttcaat gatttgcata tcgatcaaac catcatcaaa taaaatgtgt
     7561 ccgccaacgt gaacatcgtc aaataatcct gggtaagtaa ccgcgatttt ttcttttgaa
     7621 cctacatgtt caggatccat agagatgcgt gtttgatctc ctacttcaaa tttgatgtat
     7681 cctgcacgac caaaatcagc ttctgttgtg ccttgaacag ttgtacggat ttcagctcct
     7741 tttgtatcta aaagaatacc gacatctttt cctgtctttt taactgcttc acggaccata
     7801 gtcatacgtg ataattgttc ttcatggtca ccatgagaaa aattaaagcg gaagacattt
     7861 gccccagatt caatcaattg agaaataatt tctactgagt tacttgctgg tcctaatgta
     7921 ctaacgattt tggttttttt catttaaaaa actctcccat tcaattttgt ttatttaaag
     7981 ctgtacacag cttaaatagc ttggtttctt tcactcttaa aatgaaagtg aattatttag
     8041 atcatatagt gataagtctg gtttatgttt gttgttttca agtgtatcaa tgatgtcaga
     8101 cgcaacgatt tcgttgtcat ggataccgac acataatcca ccttttcctt cttgaagcaa
     8161 ttcgacagcg taagcaccga atttacttgc caacacgcgg tcgcgtgcgc ttggtgctcc
     8221 accacggaca acgtgtccca agatagatac acgtgtatga tagtcaccaa attctgaaag
     8281 tttctcagcg aattcatttc cgcccattac accttcagcc aagatgatca agcaatgttt
     8341 cttcccgcga tcgcggcctt cttggatttt tttagcaact gaagccatat caaaatcatg
     8401 ttctgggatg atgatttcat ccgctccgcc agccacacct gaccataaag cgatgtctcc
     8461 tgcattgcgt cccattactt cgataacgaa tgtacgaaca tgagaagtcg ctgtgtcacg
     8521 aatacggtca attgattcta gtactgtatt gattgctgta tcaaaaccga ttgtgaaatc
     8581 agtcccagga atatcattat cgattgttcc aggaatacct actgctggga aaccgcgttt
     8641 tgtcaaagcc attgcgccat gataagagcc gtctcctccg ataaccacta gaccttcgat
     8701 tccgaatttt ttcaattgtt cgattccttt taattgacct tcttcagttg caaattctgg
     8761 ataacgtgca gaatacaaga atgtaccacc acgttggatc ttgtcaccta catctgccac
     8821 atcaagacga cgaatatctc cagcaactaa accggcaaaa ccataattga tcccgtatac
     8881 ttccagtcct tcgtaaatac ctttacgaac aacggcacga acagctgcat tcattccagg
     8941 tgcgtctccg ccactggtca aaattccgat gcgtttcata ataaacattc acctcattaa
     9001 aatactttat cctaagtatg acttaggttt acaaccattt ttgcgttaac cacgactaac
     9061 attctaacat tatttttctc acttgtcttg atttacggat aaaaaaatga aaaaaaatga
     9121 aagcaagtag tattttttac cgtaagttat ttgaaaacca cattatcttc gccaagcaga
     9181 taaataagtt gccgctttat actcttgctg tcatccaccc aataagcatc tgacagaact
     9241 acttttcgtt gcgttttttc gtgatacata actactggta tatatcctgg gtgtttctga
     9301 aaaatcactt gtaattgttt cattatctcg ggatcttccc gttcttctgt aatacgcaaa
     9361 tagcaagttt gtgaaggata ctgggcttct aactggttag gatcagcaaa gctttctcca
     9421 attagttgca gttcctgatt atatttgctg atttcgctcc gcccttgaac aaagatcact
     9481 cgatcaacgg caaacaaatt acgatattta cgataaagag ttggaaaaac tgtgacagaa
     9541 atttctccac tactgtcggt tccttcaaca aaggccatca gttctccttt ttttgtccga
     9601 atttctctaa cttccttgat catcactaat aaattgactg gttgattagg gacgatatct
     9661 gtaatcagct gaacttgttt agctaaacga agacgatcaa acccttctgc aggatgacct
     9721 gacaaataaa tacctaagta ctcctcttcc agcttcaagc gttcttctag tgtatagtca
     9781 gcgacttctt cttcttttaa tgccatgatt cccagcaagt caagacttcc accactgtaa
     9841 acgatatttt ggatttttcc ttcgagatca agcatcaatt gtttcctgtt aggagataat
     9901 tcatcaaaaa ctcctactgc tgtcagtgat tgcagtaact ctaatttcag ccaacgctta
     9961 tcgatccgta tgaggaattg atccactgac tggaacgggc cattttgttt tctttcgttt
    10021 aatatatctt ctacaaaatc acgccgaata ccttttatcg ctgtcagtcc gaatctgatt
    10081 tcttccttat tgaccaactc aaaactggaa ttactttgat taatggacgg cgaggaaagt
    10141 ttgagtttat ttttacgtgc ttctgcaata tattctttca attttgatgt gttatgccga
    10201 acagaattca tcaggctggt aaagaaagca cctggatgat gggcttttag atacgccatt
    10261 tggaatccca cgaatgaata agcaaatgca tgtgaccgat tgaatccata gtttgcaaat
    10321 ttttcgatat attcatatac ggtcgctgct gttttttccg aatatccctg ttgtaatgct
    10381 cctgatacaa aatgcgactt ttcttcatct aatacgtctt ttttcttttt actcactgcc
    10441 cgtcgcagaa tatctgactg ccctaatgta aatccagcca tagtcgaggc gatctgcatg
    10501 atttgttcct gatagacgat cactccatat gtgtttttca aaatcgggat cagtgaatca
    10561 tctgggtagc gaatggcttc ttttcctttc ttcctggcaa tgaaggtatc tatattttgc
    10621 atagggcctg gtcgatacaa tgcattgact gcggcaatat cttcgatgtt ttctggatct
    10681 aatcttcgta aaacgttccg gataccagca gattcaaatt gaaatactcc tgaggtttcg
    10741 cctcgttgaa acaagagcag cgtttttgca tcatttagcg ggatctcttt taatcggatc
    10801 tcataccctt ctactcgctt aacagctttc aaagtaaaat caataatcga taagttgcgt
    10861 agtcctaaaa agtccatttt caaaagaccg attgcttcta cctcattcat cgtatactgg
    10921 gtcagaaaag cttcatcaga accgcgttgg agcggaacta gttcaagtag attcttatcg
    10981 ctgataacta cacctgctgc gtgagtcgaa acatgtcttg gcaatccttc taaaatcact
    11041 gcagttttga ataaaagttg gtttttttcg cttgcctgaa ctaattctac taattgatgc
    11101 gaagttttat aagcttgggt caatgtgatt ttcaattgat tgggtattgc ttttgaccag
    11161 cggtttgctt cactttgcga taaaccaaac actctagctg catctctaag aaccattttt
    11221 gctgccattg tgccaaatgt tgcgatttgt gccatatgat attgtccata cttttctcga
    11281 acataattca atacttcttc acgtcgattg tcaggaatat ccaaatcgat atctggcatc
    11341 gtaaagcgtt cggggtttaa aaagcgttcg aacagcaaat catattctaa tggatccaca
    11401 tctgtgattt ctaacacata ggctactaat gaacccgcag cagaaccacg acctgcaccg
    11461 gtaacgattt tgtttctatg agcaaaagcc attacatccc atacgatcaa gaaataatca
    11521 tcgaatccca tttgatggat gacagataac tcgtagtcca aacgttcttg gtattccttt
    11581 gtatagtcag tgattcggcg ttccacttgc gaatagcata actctcttaa atattctcca
    11641 gcttgttttc cattaggaac tggataatgc ggaagtaatt tctgatggaa ctggaattca
    11701 aatgaaatcg attcagctaa acgctgggcg cctattacag cttgggaaag acccgctttt
    11761 tcgaatcgtt tgatcatttc ttgttctgaa agaagataga catttgctgt tagtggtaaa
    11821 gatgcagata aattttctaa aggtatttgg cttccctctt tgatatgttt catcacttca
    11881 agtgcaaaaa gttcttcttg ttgtaaacta gcgatgggat gtaatgcaca aagcgaataa
    11941 ttttctttta tcacatactg ataccattca ggcaattgat caggcgtgtt ttctgtcaag
    12001 ccgatataaa aatttctagt aaaaacattt tcaagtaaaa actgccaatg accgaaaaag
    12061 ttatcctctt tattgaaagc ctcagaaaca gaatgatttg ccggaacaac aaacgctaga
    12121 tggtcggttt ctgtcatttc tgcaagttcc aatttttctt ggatcatttt tcggctggaa
    12181 agtttcatca gctgctgata acctatttca tcttttgcgt acgcaagcaa gccataattt
    12241 ttttcttcat aagtataact aagatgtaat ccaatgattg gctgtactcc ttcttctaca
    12301 caaatacgat agaattcagg tgcaccgtat aagacatctt tatccgttat ggctaatgaa
    12361 gtgtagcctt gtttttttgc ttcttgtaca tattctcgaa tacgtatcgt actttgtaaa
    12421 agagaataag aagtaattgt ataaagttgt gggaaaaaca tcttgcattc cgcctttttt
    12481 ttcgttaaaa ttcactagcg ttaaaaggtt tgatcctttt actttcatta agatagcagg
    12541 cgctgaagtt gattattcca cttcatttgc ttgcttgtaa tctgtaggaa tgagttcttt
    12601 cgcaagaact caaattttat tgagatttgg tctcgtttct ttacattcaa agaaaatctg
    12661 gtaaaattaa gatagaaaag aggtgttctt tatgcgatat atcgttacat tattctgggc
    12721 tgtcgttcta ggtcaagtag tcggttatat tggtgctgca ttaacaagcg gaacatatga
    12781 ttttactttg acaacaatca tttcattcat tgcgggtgtc attatccttt tgattggtgc
    12841 agttgcgcca agaaaagaaa cctctgccca ttcgtaaaaa aacagccaag agcttgagat
    12901 ttctcaagct cttttttatt ttctactcct caccaccatt ataatctgct gcgaagaact
    12961 ttacgaaagt tgaataaaga aagaagaata tttgacagaa caaacaaact tgtcacaata
    13021 aaaacagcac tgtaattata agcatgcgcg actgtcgatc ctagcatggg acctaaaact
    13081 tgtccaaagt tgctaaacat ctgattgtag ctatagattc ggctgactcc ttcttgaggc
    13141 gtaatcttac tgatcagtgt attgacagaa ggcatcaaag cgccagttga aaagcctaaa
    13201 ataaagcgaa gtatacctaa ttggagcggt gtagtgacga atgccatagg taaataacag
    13261 caaaatgata aaatgagtcc tccaattaga actttttgat tcccaatctt atcccctagt
    13321 tttcctagtt tcggtgaaga aaagacagcg gaaacaccag ctatcgaaac aatcaagccg
    13381 ctgacaaaaa ggatattttc tgtacttccg ctcaattcac gaatatataa ggtcaaaata
    13441 gggctgatcg tagtgattcc tatctgtaaa atcaaggtcg taatgaagag accgatcaaa
    13501 atagagagat gatccatttt tgaaaaaatt tctttggtgc ttatcagatc ttttttctcg
    13561 attggatgga aatcttcttt gactaagaaa atggtcaata ccgttgtgat catcaacaaa
    13621 gcccctgtga tcaagaatac attttccata ccaaaccact gagccagtgc accgcccatt
    13681 gaaggtccga tcagatttcc agcaatcgct ccggtagaca atgttcctaa cgcccatccg
    13741 ctcttttctc taggtgcctg tgaagcaatc atcgctgtgg catttggaat atatccagac
    13801 aaaatcccat tccaaaaccg catgatcaaa agccaatacg cgtttggtac aaaagccaaa
    13861 gatcccattg ttaatgtcat ccctgctgct gcacggatca tcatgagttt tcggcctttt
    13921 cgatcagcta gatttcccca gattggtgca accacggcag atgcaaaagc agtcacagag
    13981 attgctagtc ccgaaaacaa ttcgatttga tcttttggtg ttcctagttg ctcaacataa
    14041 actggtatga atggcataac taaactaata cttgcaccag tgaaaaaaca gccaatccac
    14101 gcaatcacta aattttttct ccaattaatc tccatctatc atccctcttt cgtctctttt
    14161 atcataccta ttttcgaata aacaagcaac gatctcgcca gtaaaaagag aaaaatcccg
    14221 caaaggtcat tttatatgta acgttctttt tatgtaaaac aaaaagagat cagaacaaat
    14281 actgttccaa tcccttctct ttagttaaat tgttgctaga atgatgaagt tgataatgaa
    14341 taaaatatca acgatccaca aagcaacaga aacttgattg tattttcctt tgattacttt
    14401 cacaattgta tagaaaataa atcctgctgc aatcccatag gaaatgctat agcacaatcc
    14461 cataaagatc gatgcaaaga aggccggtaa agcttcttcc atatctagcc atttgatgtc
    14521 tgcaaaagaa gccatcatca ttactccaac taaaatcaat gctggcgcag ttgcttgatt
    14581 tggtacaatg gcgatcagtg gagagaataa actacttaat gcgaatagca ctgccactac
    14641 tactgaagtc aaaccagtac gtccaccggc cccgatccca gctgcagatt cgacataagt
    14701 tgttgtattc gatgtaccaa agatagcccc cacagaagta gcgatcgcat cggcaaatag
    14761 tgctttatcc atttttgttt tgaacccacg accatcttcc aacgcgatct cgtcttcttt
    14821 tgagaaaata cctgtgcggc gtcctgtacc gataaatgtt ccgatcgtat cgaaagtatc
    14881 agataaactg aatgcgatga tcgtcatcaa tacttgagga atcttggaag aatcactgaa
    14941 taaagattgc atgccttcag aaccaaatgc tgcaccgaaa gttgttccta attctttaaa
    15001 tgaattacct aacgaattcg tttgccaatc aatggaactt aaatcaacaa cacccataaa
    15061 aataccaagg actgtggtcg ctacgatacc gataagaata gccccacgga cattttttac
    15121 aactaaaatg gctgtcagta ccaaaccaat cacagccaat aagataggag cgttgtcaaa
    15181 gttggctaac gcagggacga ttccaccatt tatcgtaaca tttgtcgctt ttccgccttc
    15241 aacgacagag ctcgaaatag ctgactggtc tgctgaaaaa gataagaatc ctgcattttt
    15301 gattccgaca tacgcaacga aaataccgat cccgccacca atcgcatgtt gaagactttc
    15361 gggaatcgcc cgaataatca tttttcgaat atttgtgaca gtaataaaaa tattgattaa
    15421 tccgcaaata aacaccattg ccaaggcttg ttgccaagaa taaccaagcc caaaaacgac
    15481 cgtaaaagtg aaaaatgcgt tcaatcccat accaggtgcc tgagcatatg gtacatttgc
    15541 gaacaatccc attaccagcg taccaataat cgatgcaatg attgtcgcta gaaatactgc
    15601 ttggaagggc atacctgatg ctgacaaaat cgtcggattt acgaataaga tataactcat
    15661 ggcaaagaat gtcgttactc ccgccattat ttcagttgat acatttgtat tgtttttctt
    15721 taaaccgaaa aacttttcca taaaaagttc cactccctga atttttttct gatggaaaac
    15781 tataataata aaaaataagt aaaatagggc aaatccatcg aacaatttca aattatatag
    15841 acgtttcatt cgcttttcaa cgttttttta acaaaaaaag cgaaaataat acttaacgtt
    15901 cgtgtttagc ttatgtttag ctatttatat agccataact gtagtttttg ttaggaatag
    15961 ctgattgctc ttgttaatta cctaaaatat gctagactaa aacaaatgct agttgaggaa
    16021 ggaaggaccc ttattgaata aaaaattaat tgccatcgat ctggacggaa ctaccctcaa
    16081 tcaagattcg atgattacat ctaaaacaaa agagactcta aaaaaagcaa tcaatgccgg
    16141 tcatcatgtc agtattgcta caggacgccc attccgaatg agccatcagt tttaccaaca
    16201 attagaatta acaacaccga tggttaattt taacggcgca ttagtacaca tccctaatca
    16261 gcactgggat ggtgaaagag aaacgttgat caatcgtgaa atcgcttttg aaatcttatc
    16321 gcaaaaaaag cagctaaatt tagattttat cgctgctgaa aatcgtgata ctttttttat
    16381 tgacagtttt gatttctttg atgaaaaaat ttttgcttct tctcgtccag gagagaagaa
    16441 tttgctttct cctaaaaatt tgacgacaaa tcctacatct ttgcttgtcc gaacagacaa
    16501 acgttttgca ggagctgtat ctgctgaatt aacacgtcaa tttggcagtt atgtcgatgt
    16561 tcggacatgg ggtggaccta ctgccatctt agagattgtt tcaaaaggaa tccaaaaagc
    16621 aaaaggcgtg caagagattg ctaactacct gtctatcgat caaaaagacg tcattgcctt
    16681 tggtgacgag cataatgatt tggaattgtt ggattatgca ggttggggag tagctatggc
    16741 taacggtact gaccaactca aaggcattgc aaatgatgtc actcctttga gcaatcagga
    16801 agacggttta gctgtttatc tggaaaaact acttaaacta taatagagaa ttagattcca
    16861 caaaaaagag gttgtgac
//
LOCUS       DACESX010000053        16850 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.053, whole genome shotgun sequence.
ACCESSION   DACESX010000053 DACESX010000000
VERSION     DACESX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.053"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            9..884
                     /locus_tag="IXF88_001469"
     CDS             9..884
                     /locus_tag="IXF88_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1508208.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            964..1938
                     /locus_tag="IXF88_001470"
     CDS             964..1938
                     /locus_tag="IXF88_001470"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1508209.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            2127..3263
                     /locus_tag="IXF88_001471"
     CDS             2127..3263
                     /locus_tag="IXF88_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1508210.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            3400..3744
                     /locus_tag="IXF88_001472"
     CDS             3400..3744
                     /locus_tag="IXF88_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1508211.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            4015..5301
                     /gene="efmA"
                     /locus_tag="IXF88_001473"
     CDS             4015..5301
                     /gene="efmA"
                     /locus_tag="IXF88_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1508212.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(5439..6038)
                     /locus_tag="IXF88_001474"
     CDS             complement(5439..6038)
                     /locus_tag="IXF88_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1508213.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            6442..7563
                     /gene="mnmA"
                     /locus_tag="IXF88_001475"
     CDS             6442..7563
                     /gene="mnmA"
                     /locus_tag="IXF88_001475"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1508214.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            7809..9230
                     /gene="arcD"
                     /locus_tag="IXF88_001476"
     CDS             7809..9230
                     /gene="arcD"
                     /locus_tag="IXF88_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1508215.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            9244..10362
                     /locus_tag="IXF88_001477"
     CDS             9244..10362
                     /locus_tag="IXF88_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1508216.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(10422..11714)
                     /locus_tag="IXF88_001478"
     CDS             complement(10422..11714)
                     /locus_tag="IXF88_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1508217.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            11864..12424
                     /locus_tag="IXF88_001479"
     CDS             11864..12424
                     /locus_tag="IXF88_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1508218.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            12553..12930
                     /locus_tag="IXF88_001480"
     CDS             12553..12930
                     /locus_tag="IXF88_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1508219.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            12863..14935
                     /locus_tag="IXF88_001481"
     CDS             12863..14935
                     /locus_tag="IXF88_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1508220.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(15146..16093)
                     /locus_tag="IXF88_001482"
     CDS             complement(15146..16093)
                     /locus_tag="IXF88_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1508221.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            16339..16803
                     /locus_tag="IXF88_001483"
     CDS             16339..16803
                     /locus_tag="IXF88_001483"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1508222.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
BASE COUNT         5502 a         3028 c         3606 g         4714 t
ORIGIN      
        1 cacttataat gaaaaaaagg aggcgatccc aactgaatca agtagtggat aaacctttat
       61 atttcaaagt agataaaaaa attaaaaaat tagccagcac tcagcagctg cagtcaagaa
      121 aaagtaaatt gcttttttta gctctggttt ttgaagatca aagttatgtg attatcgatc
      181 aatcaggaca tccggtagaa tattctccag caaaatatac ttatcaagag ggtctttctt
      241 gtaaaaaatg gaagctgatc aatgaagagc caatcgagtt atctagatgg atcaacagaa
      301 aagaagatat tcccgttttg atcgaagaaa aaaggtcagg aaaggaactc gcaaattgct
      361 gggtgggatt gccagaagaa cgttttttga ggtataagaa atgggctacc ccctcaggct
      421 atctttgtgg tacttacgcg gcagcagttc tccttgccta ttatcaagac tatcgtaagg
      481 ggtggatgct tccaaatgag attcgcaaga aaaatacggc tgattcgttg gttttgacga
      541 aggcattgcg tagccagatc caaccactag gacttcctac gattcctttt caggttagta
      601 cagggatttc tagtttttta aagaagaatg gcaatcatga gcgtgcacga gcaactcttc
      661 taggttcatg gcagcgagct acaaaacgta tacgtgaagg caaacctgtg atgattggta
      721 ttttgaaagt acttggcagt acatatggca atcattgggt gacagcgtac gcttattttg
      781 aaactgaaac aggagaacgt tattacaaag tacacgacaa ctggggcgat tatcataaag
      841 tcattcctgc cagttggagt aacggaacag tttcacttcc ttaagaattt gctgaaatta
      901 acagaaaatc ttggaaaaat atgttaaact atcgaagaat agagaagttt tggggaggac
      961 tacatgagta ataagtatca ggatgacacg ttgaagattt ttagtttgaa tggcaaccgt
     1021 ccattagctg aaaaaatcgc gaaagtgttc ggaacagaat taggtaaaag cgtcgtgaag
     1081 caatttagtg atggcgagat ttccatcaat atcgaagaaa gtattcgagg ggatcatgtg
     1141 tatatcgttc aatcaacgaa tgcacctgtc aatgattact atatggaact actgatcatg
     1201 atcgatgcaa tgaagcgagc aagtgcgaaa acgatcaatg tcgtattgcc ttactatggg
     1261 tatgctaggc aagaccgcac ggcgaaacct catgaaccga tcactgcgaa attgatcgct
     1321 aacttgatcg aagaagcagg ggcaactcga gtactgactt tagatctgca cacggttcaa
     1381 gtacaaggat tctttgatat tcctgtagat aatttattta cgatgccgtt gtttgctcat
     1441 tattaccgtc aattaggtct gacgggagat gacatcgtag ttgtttcgcc aaaaaacagc
     1501 ggtgttcagc gtgcacgtag tctttcagaa tatttgaaca gcacattagc catcgtagat
     1561 catgcagatg atgcttcaac tgagggtggt tatgtgatcg gtgatgttca aggaaagaca
     1621 tgtatcatgg tagatgatat cttgaacaca ggagcaacgc ttgcacgagc agcaaatgta
     1681 ttaaaagaaa acggcgcaaa agaagtatat gcctgtgcgt cacatggatt attgtccgat
     1741 catgcgaaaa gtattttaga caatgcacca atcaaagata tttgtatcac tgattctgtt
     1801 ttcacagaag aaaatcgtca tccagaaaac ttaacaatcg ttacatgttc agaattaatg
     1861 ggtgaagcgg tcaaacgcat ccatgaaaat acaccgatga gtccgttatt ccgtctggaa
     1921 gaaaaagaat tcgaataaac aaaaaaaaga gggaaagaag ttgtgacaga agtgagcagc
     1981 ttcaaggagc tgcgacaaga cttttgtcac agctcctttt ttctatgccc atgaaacgtc
     2041 ctctagacaa aaatgagctc ttctttggtt tgttcgctat tttatgctag aatgtgccta
     2101 gcaataattt ggaagggatg aatgaagtga aaactattta tctggatcat gcggcaacta
     2161 cccccatgca tccacaagta attgaagcga tgacggcaat catgcaagac acgtttggaa
     2221 atccttcaag catacatgga tttggtcgcc acgcgcatga aaaattggaa acagcaagac
     2281 aaatcattgc agacagtctg caagcaaaac ctcacgagat catctttaac agtgggggaa
     2341 cagaagggga taatacagct atcttagaaa cagcgttctc gcgtcaaaaa gaaggcagac
     2401 atctaatcac tacagctatc gaacatcctg ctgttcttaa taccatgaaa tatttagaga
     2461 caaaggggtt tgacgttaca tatctgcctg tagatgaaaa agggaatctg tcgatcgaag
     2521 aggtcaaaaa agcgttacga gaagatacga tcttggtctc tgtgatgtac gggaacaatg
     2581 aaatcggaaa tttgatgcca atcaaagaaa tcggtgaact tttagcggat catccagctt
     2641 atttccatac agatgcggta caagcgtacg gaaaccaaac gatccatcca gaagaattag
     2701 gaatcgattt attaagtatc tctgctcata aaatcaatgg gccaaaaggc gtcggtttct
     2761 tatataaacg agacggagta aatatccctg cattgctttt aggcggggag caggaagaaa
     2821 agcggcgtgc aggtacagaa aatttagcag ggatctgggg tatggcaaaa gcagttgaac
     2881 tgctgtcacc tgaagaacgt gaagcacgta acaacaaata taaaggattc cagcaattga
     2941 ttcttgatac attagaagca aacgaaatcc cttatcaagt aaatggtgat ttaacgaata
     3001 aactccctca tgtgttgaat cttcgcttaa ttggcgaagc aaatgatttg ctactgatga
     3061 agcttgatct taatggggtc gctatttcta ctggttcagc ttgtacagca ggaaatatcg
     3121 agccttcaca cgtattagaa gcaatgtatg gagaagatac accgatacta aaagaatcca
     3181 tccgtatcag ctttggtttg ggaaataccg aagacgacat ccagtatttc cttgatcaat
     3241 tagttcgtgc tgtcaaaaaa taactagtga agtcattctt atatgaacag atgtctgaga
     3301 gaacgccttc attttttagg gaacttaact gaaaaaccta gtaaaaatca ggataaactt
     3361 ttataataaa gagaaactaa ttttatcgag gtgaaaatca tggcatttga aacaacagca
     3421 acggtactgg gcgcaccaat tagctatcgt ttgcatcctg atgcaaaaag atatacattg
     3481 cgcgataatg gatttacaga aacaaacgga ggcaattatc aattgatccg accattagat
     3541 gccacgccgc aaagcaaaga aggattcaaa ttaaaaatca ctgtatcaaa agatattcaa
     3601 acattgaaaa tgtcgatcac tacagcaaat ggattgaaag cagtcaatat tttcaaagat
     3661 ccaaaacaga aaatgagcca agataaattt tattttctga tggatgggat gattagccgt
     3721 gggttattcg aaaaagtcga atgatctgtg gatataaaaa gacaactagt tttgaaacgg
     3781 cctattgtta gatgactaac tttgtaagga cgacgcaaat tagttgtctt ttttattttt
     3841 aaatttaaca catgttgtta tttttaatat tttgctgatt tattgtttct aagtaaagaa
     3901 atttaaattg aaacgattgt tatttaggga aaaaggtatt ttttgaaaaa agaatcccgt
     3961 ataatgattg ttgtgcaaac actatacaaa atgaaaaaga aaagagggat gtttatggaa
     4021 aatgaacagt cggtcgtttt aacgaattgg aagagaaatt atttgttctt tttatcggga
     4081 cagtttttgt caggaatcac tagtatggtc gtccaatatg cgatcatctg gtatttgaca
     4141 agagaaaccg gttcggcgac aattttgagt tttgctactt tactaggaat gattcccatg
     4201 gttttactaa gcccatttgt aggaccgcta gtggatcgct gggataaaaa agctcttttg
     4261 attgtcacgg atattattgt agcgattttc gcgcttattt tagcagtagt cggaacgatt
     4321 tcagaatcct tcccgatttg gctagttttt gtgtctttgt ttatgcgttc ggtcgcacaa
     4381 acattccaaa tgccaacgat ccagtcgatt atgccgacaa tcgttccatc ttctcacata
     4441 acaaggacga atggacagtt aggaatggtc caatcagcga atttcatcat tgcgccagct
     4501 ctaggtgcgg ctttgttttc agtcgttcct gtcaattacc ttattttatt agatgtattg
     4561 ggggcagttt ttggtgtcgg cttattgatt tttgtgaaga tcccaaaagt ttctcctgaa
     4621 atattggaag ttccgcttac tatttttaaa gatgcaaaat ttgggctgca gcaattgatg
     4681 gataataaag ggttatggta cataacgatc aatggtgcat ttgtgatgct gttgttcatg
     4741 ccagcaatca gtctgtatcc attgatgacg ctagattact ttggtggttc cgttggacag
     4801 gcgggagcag tggaagtagt ctacgcagtc ggaatgctct taggcggtgc actgatcagt
     4861 tttataggaa catggaaaga ccgaatgaaa cctattatta tagcctacat cattatgggc
     4921 ctgacgatcg gtgcaagcgg attggttcca aacgatagtc aagggttctt gtactttctt
     4981 atcttgaatg caggagcagg ttgtgcaacg ccatatttca atacactgct gatggcaatg
     5041 atccagcaga gttatgaatc gaatgtttta ggtcgtgtct tagggaactt caactctttg
     5101 atgaacctgg caggtccaat tggattatta tttgcaggac cattagctga tcgtttaggg
     5161 gtagaaaaaa tgttcttgtt ttccggaatc gggattttat tatgcggaat cgttttattt
     5221 ttaacgtcag ctgcccgaaa ttatgataaa gaattacaga aaaaactggt aaaagaacac
     5281 catgagcaaa aagacgaata gtatggaatg gagttgtgac agaagtcttg ttacaactcc
     5341 atttctgatc ctaatgttcc tgtggcaata agcctactca aatgaaaaat atgagtaatt
     5401 atctttattt gtttaatact tgatataggg cagttttttc acgacctatc gtacttcctc
     5461 tttgcttcat agatttcttt gatttgttgc tcagataacg gagtgccaaa tggtagtttt
     5521 tcctttaaga gttctctttc cggtgcgtct acgcctacat tgacatcgtt ctcgataggt
     5581 acgctgtaat gatggatatg tccatgaaga ttgcggatag tgtcgttagg cccgagcaac
     5641 atcggataat gagtcaagta atattgttga tggtcgaatt taacgatggc acctacatca
     5701 taaaaataaa atttgggact tccgtcagta agcctaggat catttttttc taaataacgg
     5761 aaaaaagcgc gactgtcatg gttccctttg atcaagtgga ttgttccttg cagttgagtg
     5821 accagttcca aaatttctgc atttgctttt tcatattgcg gatgcatagc aaggtcgcct
     5881 agatgataaa catggtcggt ttcttttatt actgcattcc agctagtgat caactgttgg
     5941 ttcatttcat ccacatttgc aaagagccgc ggagcaaagt cgttttttcc taataagcgt
     6001 tcatggaaaa agtgcatatc actgataaag taattcatgt atcttttgcc tccctttttt
     6061 cttagagtaa acaaaatcac ttatctgagc aactcataac atctaaaaag aaaaggttgt
     6121 aaatgagacg tgcagcttta agcgataaga agcaattttg aaaaatagtt tttcacaatt
     6181 tataaaatta ttccttattg ccgaaaggct ggcccttttt atgagtatgc caatttttta
     6241 tatcgaatgc tcaaaagatg agtgattttt tggttgacta tcatgtttgt gtactgaata
     6301 ggtccagtag aacagtattt ttacgacttg ataattaagg gatgaaattt tcagaaatat
     6361 gacgtataat gtaaaatact gaaaagttgc gaccttcaac tcgtagatgt ttcagaatct
     6421 aattgaaatg atggtgatat catgacagac aacagcaaaa cacgcgttgt cgtagggatg
     6481 agcggcggtg tcgactcatc tgtaacagca cttttgttaa aggaacaagg atacgacgtg
     6541 atcggaatat tcatgaaaaa ttgggatgac acggacgaaa acggtgtatg cacggccacg
     6601 gaagattata aggatgtagc gaaagtagct gctcaaatcg ggattcctta ttactctgtg
     6661 aattttgaaa aagaatattg ggatcgtgta tttgaatatt tcctagccga ataccgcgca
     6721 ggacgtacac ctaacccaga cgtgatgtgc aacaaagaaa tcaaattcaa agcattcttg
     6781 gattatgcaa tggatcttgg cgccgattat gtagctactg gacattatgc gcaagtcact
     6841 cgcgatgaaa acggcgtggt ccacatgctt cgtggagtag acaataataa agatcaaact
     6901 tacttcctta gccaattatc acaagaacag ctttcaaaaa cgatgttccc tctaggcggc
     6961 atggaaaaat cagaagtacg ggcaattgct gaacgcgcag gccttgcgac tgctaagaaa
     7021 aaagattcaa cgggaatctg ctttattggt gaaaagaact tcaaacaatt tttgagcaac
     7081 tatctgccag caaaaaaagg aaatatggtc actcttgatg gtgaagtcaa aggacaacat
     7141 gctggcttga tatattatac gattggtcaa cgtcaaggac tagggatcgg tggcggagga
     7201 gattcgcaag aaccctggtt tgttgtagga aaagatctag cgacaaacac attatatgtg
     7261 gggcaagggt tccatcatcc tgcattgtat gccacaagct tggatgcaag tgaaatccac
     7321 tttacgacga atgaaccaat gccaaaagaa ttcaagtgta cagcgaaatt ccgttatcgt
     7381 cagcaagacg ttcctgtgac agttcgctta ttggatgata atcgtgcaga agtcgtattc
     7441 gacgaacctg tacgtgcaat cactcctgga caagcagttg tattttatga tggaatggaa
     7501 tgtctgggtg gtggtttgat cgaccacgca tatcaagaaa caaaagtttt acaatacgtt
     7561 taagcgatat taaatgaatc aaaacagcta tggctaacaa aagaaacacc ttttcttttg
     7621 gatagcatag ctgttttttt gcaataaaat taatttttat tcattttata cttgaaatac
     7681 ttgtgtaaaa agttcataat atccaataat atgaataaag ttgctttttg ttaagcaaga
     7741 gcagttgcaa aatagaaggg aatcttttgc aagtgatttg ttcaatttta taagtgaggg
     7801 tgatggaaat ggaaaagaaa tcgacgggta ttagcaaatg gggactggtt gctctggttg
     7861 tcagttcgtc aatcggaagc ggtgtgtttg ggatcacaag tgatctggct agttctgcag
     7921 ctccagggcc agcgatcctt tcatggataa tcgttgggat tggtatttta gctttggtat
     7981 tatcgctgaa tcatttagga gaaaagcgtc cagatctaga tggtggtatt ttcggttacg
     8041 cggaagcttc atttggtaaa ttaggtggct tcatcagtgg ttggggttac tggttgtctg
     8101 cgtggctggg aaatgttgct tttgcaacga tgctgatgag cgccatggga gaatttttcc
     8161 cgatattcaa aggtggacaa aatgttcctt ctattttatt tgcaagtatt tttatttggg
     8221 gtctgacttt attagtaaac aatggagttg aaggagcgag cgtcatcaat acaatcgtga
     8281 cgatctgtaa attagtgcca ttattcctat ttttaatatt tgtatttatc gcatttaaag
     8341 tgaatatatt cactgctgat ttttggggta acgtatctga taatctagtg aatggaacag
     8401 ggcagcaagg aacgatctgg ttgcagatta aaggttgttt gatggttatg atgtgggtct
     8461 ttgtagggat cgaaggcgca agtgttttag ccaaccgcgc gaagaaacgt tcagaagccc
     8521 agcaagcatc gatcattggt ctgctttgtt tattgtttat ctatatcctt gcttcgatct
     8581 tgccatacgg cgtcttatct caagaagagt tagcaactat ttcacaacct gctatggcaa
     8641 atattttaaa aggaatcgtg ggaaattggg gagcagcgct gatcaatatc ggcttgatta
     8701 tttcgatcat tggttcttgg ttgtcttgga cgatgctccc agcagaaaca acgatgttga
     8761 tggctcgaga cggtctgttg ccgaaaggtt ggggaaaaac gaataaaaaa aatgccccaa
     8821 cttattcgtt agttgttact gcaatcttga ctaatctatt cttattgact ttcttagtta
     8881 ctgattatgc ctatcaattt gcctattctt tatgtacagc ggcaattttg atttgttatc
     8941 tacttgtcgg gatctatcaa gtccagtttt ctttaaaaga aggaatcaaa aaacaagtag
     9001 tgatcggtgg gattgctgta gtttttgaat tgatgggcat cattttagca ggattttctt
     9061 atgtcctttt atgtagtatt gcctatcttc ctggtttttg cttctactgg atcgcttgtc
     9121 gtgaaaccaa acatcaaatt acgggaaaag aaaaagtaat gattgggctg atttgtgtgg
     9181 ccgcactgct ttcaatcggg ttgcttgctg taggctggat caagatataa gggggaaaga
     9241 aacatgaaaa tcgcaaattt cggtgtagaa gaatggctaa ataaatggga aaagaaagcg
     9301 gtatatgata ttgcacaaag ctcgatcgaa gcattgacat tagacgaact cgttggctta
     9361 gatgggacta gcgtaagtaa atttttcgaa aaacgcaaaa acgaaccatt ggactatggc
     9421 tggatcgaag gatcagatgt tttcaaacaa gaagtcgctt ctctttatca aaccgtcgat
     9481 ccggaaaaca tcttacaaac gaatggagcg actggtgcta atcttttagc tttatacgcg
     9541 ttagtggaag caggagacca tgttgtttcg atgcttccta cttatcagca attatatgac
     9601 atccccaaat ctttaggtgc cgctgttgac tttgttcatt tgaaggaaga agaagattgg
     9661 cagtttgatt tagaacagct agaagcctta gtcaaacctg aaacaaaaat catctgtttg
     9721 aatagtgcaa acaatcctac aggcacattg ctagatcgga aaactttaga aaaaatcgca
     9781 gaaattgctc gtacagtaga tgcatatgta ttgatagatg aagtatatgc gcctttaacg
     9841 gataaaggtg aattcttgtc gatcgtagat gtctatgaaa aaggtatagc aaccaattcc
     9901 ctatccaaaa cctactccat tccaggtatt cgtattggct ggacagctac tggacctgag
     9961 ttggcagaag tttttcgaaa gtatcgagac tacacgatga tttgcggcgg agtcctgtca
    10021 gatgatcttg ctgtacatgc attgaagaat aaagaaaaaa tattggcgcg aaacaaaaag
    10081 atcattacag agaaccttgt gattttaaaa caatgggtag ccaatgaacc gaaagttgaa
    10141 cttgtcgctc ccaattacgt ctctacttct ttcattaaat tagccatcga agaagatgac
    10201 cagacatttt gtatcaattt attagaagaa acaggagtac tattagtacc aggaagtgcc
    10261 tttgatttac cgaagcatgc ccgactaggt tactgttgta aaaaagaaac cttggaaaag
    10321 ggattattcc tcttatctga gttcttgaaa aaacaggcct aaacaaaaac actgcaagga
    10381 cagtctgtga caactgttca agcagtgttt ttcatttacg cttattgtct atgagaagga
    10441 gagtgccctg aagctatgga gactcctgaa gctttttctt ctgcagtttc ttgctgatgg
    10501 atctcttgtg tattgtgtcg tacaagcaag aagttgacca aaacaagaag agaagtcgaa
    10561 agaaagacgc cacgataacc aaatgcactg gaaacactgg agccgatcat aggccctact
    10621 acgttaccag ttgcttggaa agattggtta taactgaaga tccgtccagc agcttgatga
    10681 ggcgaatatt ttgtgatcaa tgcttggaca gcagggagca gacaagcatc agagatacca
    10741 atcaaaaaac gtaaggcagc taattgccaa acattcgtga cgaatgccat tgggatataa
    10801 accagaatcg caaaacccaa accaatcgca agtattcttt cactcccaat cttgtcacct
    10861 aagcgaccaa atcttggagc ggctatcaaa gtggcaatcc caggtatcga agcaatgatt
    10921 ccgctgatca acgtaacatt cccatgtcca tgcagcaatt ggcgaatgta cagactgatg
    10981 attggactaa tggaattgtt ggatgcttgg atgatcatcg ttgtgacgaa cataccgatg
    11041 acgacgtgtg gatatttcaa gtctttgaaa atctgtttgg cagaaaccat gttttctttt
    11101 ttcactggga caaatttttc atggacgaaa aacagactca acaagaagac gaaaaataag
    11161 atgatccctg taatgaaaaa ggtagggcga tagccaaacg ctgaagcaga gatcccgcca
    11221 agaagcggtc caagtaatgt tcctgtaaca gaaccagttg ctaaggttcc aagaacttga
    11281 ccactttttt cttttggcgt cccagttgcg acaagggcag tggcattact gatataacca
    11341 gaaaaaatcc cttgaagcag acgcaatgcg actaattgat acacactagt cacgcaaccc
    11401 atcaaagaaa tgacgattgc cattcctaat gaagcacgca acagcatcaa ttttcttcct
    11461 ttttggtcgg ctaattttcc ccaccaagga gagataagcg tagtcaccaa gaaagtagat
    11521 gaaaaagtca atccgctcca aaagttcaat tgtgacgtac tataattacc taacgtatct
    11581 atgtaaagtg acataaaagg catgactaaa ctaaatccga taccagccat aaatgttcca
    11641 aaccataaga caaacaagtt ttgttcccaa gtttctcttt tcctaaataa tttttccttt
    11701 attcttgcag ccaattagac tccttctttc tgaaaatttt catctacagc tctttattat
    11761 aaactacaat atgtagttta tcaatgaaag tctctttttt ctctttcttt ttcgtggtaa
    11821 actgaaagag tgaaggattt attttatgag aggaaacatg gaaatgaaaa aaagaaatct
    11881 gtctcaagag aaaatcatcg actgctttcg agaactagca gaagagatgg atgttcagca
    11941 aataaccttt cagcatttag ccaaacgctt ggatatcaag gctccctcgc tgtataacca
    12001 tttcaaaaat attcgtgatg tcaaaacagc gctaacagcg aaacttctgc aggaacttaa
    12061 tgaccagctg cgacgggctc ttgtgggaaa aagtggtcag gaagcactgc agatctatgc
    12121 agaaacttat cgatcctttg ctttttcaaa tcaagcagtg tatgagttac tgatcagtgt
    12181 accgcatacc aatgaagctg ttttattaga tggaatccat gaaacgaatc agatcatttt
    12241 gcagatattt gaggcttttc aattaacgaa agaagaaaaa gttcatcgaa gcagggaact
    12301 tcgtagtatg atccacggtt atctttcctt acgtttttta ggatacttta caaaagaacc
    12361 aacaatcgat ccagaagaca gctatttttg gatgatcaat aactttatcg ctactttgcc
    12421 ttaaacaatc agattcattt gtaacaattt taagatgttc attaaatgac ccttttataa
    12481 gagaaaattc ttgcaacttt cttgaaaagt gactaagatg agtctagaaa agctgaaaga
    12541 aggagcacga gtatgtatca agtaattacc atgttcgggg acaatgagcc ttggtggttt
    12601 tttgaagact gggaagaaga tattgaagaa gaagaaacat tcgagtcttt cgaagaagca
    12661 cgacaagcgt acgaaaaaca atggaacgaa atccatcaaa attatgaata tatcaatgca
    12721 aaaagtaact ttctcagcgc cttttggaat gacggagatg agcgctggtg tgaagagtgt
    12781 gacgatgatt tacagcaata caagggactt gcgttgttga aaaatcatca gcccatcaca
    12841 gtagaaagca gaaaggaatt ttatgaaaca actaattcta gcggaaaaac caagcgttgc
    12901 gaaagaccta agcaaggtgc ttggtgctaa tcaaaagcac aaaaattatt atgaaggacc
    12961 caaggtgatc gttacttggg cccttggtca tttattagga ttgaaaatgc cggaagatct
    13021 gaataaggaa tggcagagct ggcaaatgga cacgttgcca atgatcccta aaaaactagg
    13081 aatcaaacca cttcctaaaa caggacatca attaaaagca atcaagcagc tggctcagcg
    13141 aaaagatgtc tcagaagcag tcattgcgac agatgccgga agagaagggg aacttgttgc
    13201 tcgctggatc ttagagtatg tgcatttcaa taaaccagtg aagcggttat ggatctcttc
    13261 tcaaacagat aaagcaatca aaactggatt caagcaacta agacctgcga aagaatatga
    13321 tgcattgtat gaatctgctc ttgcccgggc aaaagcggac tggctggtag gattgaatgt
    13381 gaccagagcc ttaactgtga agtacgcgga taatttatca gcaggccgtg tgcagacacc
    13441 gacactagca ttagtgagag accaagaaaa gaaaatcgag cagttccgtc cacaaacgta
    13501 tttcacgatc acgttgcaag ctggaaaaga acaagccaaa atgcttcaaa aaaatcaatt
    13561 tgctttaaaa acacaagaag aagcacaaaa acttgttcaa ttgatcagta caagcaaagg
    13621 aactgtcaca gaagtccaag aaaaattaaa aacggaatct gcgccattgc cttatgattt
    13681 aacagagatc caaagagaag ccaatcaacg ctatggctat tctgcaaaga aaacattaag
    13741 tcttgtacaa agtttgtatg agacccataa gatcgttact tatccgcgta cagacagcaa
    13801 atatctgaca aatgacatga aaagtacgat gaaagaacga ttgcaagcag tcagcgactt
    13861 tgctcctgag gtcaagcagt atttgaaaaa cggagccatc gtccaacaga caaaagtatt
    13921 ccaagacagt aaagtgaccg atcaccatgc acttttgcca actgaaaaca gagcacgtta
    13981 tgaaaaatta agtaatgaag agcgaaaaat ctatcaaatg attgtctctc gttttttagg
    14041 actgtttgct caaccccata aagtcagcca aacaaaagtt acggttgaat ttggtaaaga
    14101 acgattcatt tttcggcaaa atcgtgtcgt ccaagcagga tggaaaggcg aaactgaaac
    14161 tgagacggaa acagtcaaat gggaaaaagg aatgagaatc aatcccgatt tcgccatcaa
    14221 aaaagaacta tctgcaccgc ctaaaccatt gtcagaagca agcttgcttg gtcaaatgga
    14281 aaaatacagt ctgggaaccc cagctacgcg tgcagagatt atcgagaaac taatcaaatc
    14341 tgaactgatg gaacgtaccc ctcaaggact gcaagtatct cctaaaggta agcaattatt
    14401 agtattggta aatccctctt tagtaacacc agatttgaca gaaagctggg aaaaagacct
    14461 tgaagctatt gctgcaggaa agaaacaagc aggaaccttc ttgaatggaa tcgaaaaaga
    14521 aacaaaacgc ttggtgaatg agatcaaatc aagcaaacaa gaatatcaag atttctcgat
    14581 tacccaaaag aaatgcccag aatgtggggc caatctgcgc gagaaaaaca cacgtgacgg
    14641 caaaatctat gtctgcacca attcagattg caactaccgc agaagaaaag atccaaaagt
    14701 ctctaatcat cgatgcccgc aatgtcaccg gaaaatggaa atcatcgaag gtaaaaacgg
    14761 tgcttatttc cgttgtaaat acgatggaac gacagaaaaa ttgcttgata aaaaagagca
    14821 gaagaagaaa atgaccaaac atgaagaacg acgcttgctg aaaaaatatg cacaagcaga
    14881 agaaccagaa gaaagcccat tagcagcagc actaaaagca gcaatgaaga agtaataaga
    14941 tatgaaagag gttgtgatag aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa
    15001 ttgcttctca aatttttgtg aaatttcggc ttatttccga agaagctact tctggaacac
    15061 catttattcg gaaaaaagga ctgtgacaag acttttgtca cagtcctttt tcatttaaaa
    15121 aaatgtttac ttcatttgac gtatattatt ggtgaggctt caattcattt ggcttcactt
    15181 tttctgtttc tcttatgata taaacaggac gctgtttcgt ttctaagaag attttcccaa
    15241 tatatttccc gatgattcca agacataaca attggattcc accaatgaac aggaagatcg
    15301 agaccatgga aggccatcct gatgtaggat caccaaacaa caatgcccgc aaaataatga
    15361 acaaaattgc aataccagaa ccgacacaag acaaagcgcc gataaatgaa gcaatcgtta
    15421 acggtgcatc cgagaagttc acgataccat caatcgagta gttgaataat ttccagaagg
    15481 accaagatgt ttcgccagcc acacgttcgc ggttttcata agaaatatat tctgttttaa
    15541 atccgaccca gctaaataat ccttttgaaa atcggttgta ttctgttaat tctaggacgg
    15601 catccaccat ttgacgagtc atcaagcgga aatcacgagc gccatcgacc atttctgtat
    15661 cgctgatttt attgatcaac tggtagaatt ttttcgcaaa gaaactgcgt atcgcaggct
    15721 cgccttcacg agtcgaacgc cgggtgccga cacaatcgat atcagaagta gtgatccggt
    15781 tgatcatctc aggaagcagt tcgggcggat cttgcagatc aacatccatc acggtcacta
    15841 aatctcctgt agaagcttgc aatccagcgt acagagccgc ttctttccca aaatttctgg
    15901 aaaatgagac aaagcggaca taaggataag ctgaggctag cttcttgatt gctgctaacg
    15961 taccgtcttt tgatccgtcg tcaataaaca aatattcgaa ctcatgggca cctttgaatt
    16021 tttcaacctc atcaaaaaaa agaggaacag ttttttcttc attaaaacat gggacaacaa
    16081 ttgatacttt catcgtttct atcctttcaa tcgtatgttt atatatggtt tacattcaaa
    16141 atttaataaa ccttaaataa cacctttaaa atttgatttt acgtaaaaga acaggtattc
    16201 tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg gtttatcaaa cttttaagaa
    16261 caatttttat tagttggcaa gtagttttgg atagagaaaa aggaggaagt tccagtctat
    16321 tgactaggag caaacaaaat gaaaatacac acacctatcg gctttttatg gttagatgca
    16381 gatgaatccg gattgactgc tgtatctttt cagccaattt cagaaagcga atcagcgaat
    16441 cagcgaattt taacgcgcac acaagaacag tttgaagaat attttgcggg aaaaagaaaa
    16501 gaatttgatg ttcctttatc tataaagaga ggtacgcctt ttcagcaaaa tgtctggaaa
    16561 gctttgtcga ccattcctta tggagaaatc cgaacgtata aagaagtagc cgttgctgtt
    16621 gatagtccga aagctgtgcg agcgattggg caggcaaatc gaaccaatcc attgccaatc
    16681 gttattcctt gtcatcgagt gatcgggcaa aatggaaaac tgacaggata tatgggaaac
    16741 gcaatagatg gggttgaaat caaacgccaa ttgttgttgt tagaaggata tctgcaaaat
    16801 taaaaacagt tgtgattcgg acattgttcc atgcgagaat cacaactgtt
//
LOCUS       DACESX010000054        16375 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.054, whole genome shotgun sequence.
ACCESSION   DACESX010000054 DACESX010000000
VERSION     DACESX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.054"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            108..1259
                     /locus_tag="IXF88_001484"
     CDS             108..1259
                     /locus_tag="IXF88_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1508223.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(1368..3407)
                     /locus_tag="IXF88_001485"
     CDS             complement(1368..3407)
                     /locus_tag="IXF88_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1508224.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETVVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3465..4022)
                     /locus_tag="IXF88_001486"
                     /pseudo
     CDS             complement(3465..4022)
                     /locus_tag="IXF88_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(4561..4767)
                     /locus_tag="IXF88_001487"
     CDS             complement(4561..4767)
                     /locus_tag="IXF88_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508225.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(4783..5397)
                     /locus_tag="IXF88_001488"
     CDS             complement(4783..5397)
                     /locus_tag="IXF88_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1508226.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(5544..6227)
                     /locus_tag="IXF88_001489"
     CDS             complement(5544..6227)
                     /locus_tag="IXF88_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1508227.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(6336..6794)
                     /locus_tag="IXF88_001490"
     CDS             complement(6336..6794)
                     /locus_tag="IXF88_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1508228.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(6878..8641)
                     /locus_tag="IXF88_001491"
     CDS             complement(6878..8641)
                     /locus_tag="IXF88_001491"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1508229.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(8862..10322)
                     /locus_tag="IXF88_001492"
     CDS             complement(8862..10322)
                     /locus_tag="IXF88_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508230.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(10530..11456)
                     /gene="glsA"
                     /locus_tag="IXF88_001493"
     CDS             complement(10530..11456)
                     /gene="glsA"
                     /locus_tag="IXF88_001493"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1508231.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(11479..12906)
                     /locus_tag="IXF88_001494"
     CDS             complement(11479..12906)
                     /locus_tag="IXF88_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1508232.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(12937..14151)
                     /locus_tag="IXF88_001495"
     CDS             complement(12937..14151)
                     /locus_tag="IXF88_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1508233.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(14492..14734)
                     /locus_tag="IXF88_001496"
     CDS             complement(14492..14734)
                     /locus_tag="IXF88_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1508234.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(14766..15323)
                     /locus_tag="IXF88_001497"
     CDS             complement(14766..15323)
                     /locus_tag="IXF88_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1508235.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(15336..15524)
                     /locus_tag="IXF88_001498"
     CDS             complement(15336..15524)
                     /locus_tag="IXF88_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1508236.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(15537..16103)
                     /gene="amaP"
                     /locus_tag="IXF88_001499"
     CDS             complement(15537..16103)
                     /gene="amaP"
                     /locus_tag="IXF88_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1508237.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         4653 a         3323 c         2765 g         5634 t
ORIGIN      
        1 aaaaatcttt tggattgttg gtttttttgt gagtatattt tcctattttt tcaataatgt
       61 atgttatgct aagttcgggc tatattttat acaaaggaat gatgagaatg gaatttattg
      121 gtattttatg cctgatattg atttcaacaa cgattggctc acatatctcg cggagattag
      181 gtatcccagc agtgattgga caattacttg ttggtgtttt gctaggccaa gctggattga
      241 attgggtcaa acctgatatt ctagtccatg acttttcgga gattggtgtg atcttattaa
      301 tgtttttagc tggtctggaa agtgatttat ctcttttgaa aaaatatttc cgacctggta
      361 tgttcgttgc tttgcttggg atcatcttcc cggttgtttt tggctggttg acaggcaaag
      421 cattccaagt ttcgggaaat gaagcgattt tctttgggat tattcttgcg gcaacttctg
      481 tcagtatttc agtagaagta ttaaaagaac taaacgtagt gaatacaaaa gaaggatcaa
      541 caattttggg tgcttctgtg gtagatgaca ttttagtcgt tttagttcta agtttcagcc
      601 tatcttttct aacgggggaa gcttctagca atgcttcttt accgcttgtt ttgattgaac
      661 agctaattta ttttgttttt attttcttat tagtcaaatg gattgcgcct ttcctacttt
      721 catttgcaga aaaagtgtac gcaaattctt ctattatcat cgtatcgcta gttatttgtt
      781 taggaatgtc ttacgtagcg gatcttgttg ggcttagctc tgtcattggt gctttttttg
      841 ctgggatagc tgtcagtcag acaaaggtta aagaagaagt ataccacaat gtagaggctt
      901 taggatatgc agtatttata ccagtatttt ttgtcagtgt aggattagaa gttgattttt
      961 ctgactttag tgaacaacta ctctttattt ctatattgac gcttgtagcg attttgacca
     1021 aattgcttgg aggttatttt ggtgctcaga ttgccggttt ttctcaacat agttcattga
     1081 tggtcggtgc tggtatgatt tcgcgtggag aaatggcttt gatcatttta caaattggac
     1141 agcaaagtca attgatcgaa ccacagtact attcaccttt agttatcgtg attttattaa
     1201 gtacacttat ttctccactt gttctcaaat attttacaaa aaaagtttat gaagtgtgag
     1261 taaatcaaaa agaagttgtg aaagagacat cgtgaaaacc gtttatttga aaaaaggact
     1321 gtgacgataa cgccacagtc cttttactgt acttaaaaag caatttttta ttgataattt
     1381 tggttgaggt attgcaatag ttccgatgct cgtttggttg ctgaatcatc atcttcttta
     1441 ttttccaaca tgctgaccat catataacct tggttatcaa gattgaaagc aaacaagaaa
     1501 ctgttctctt gcccctttac atcctgtttt tctttgattt ccgctgtacc agttttcgct
     1561 gccaacggaa tccctaaagc agaaagagaa tgtgctgtac catttacatc ttgcacaact
     1621 tctctcagat ctggcacgat cgtttgtact actgtttcgc tgatgacatt cttcttatct
     1681 tttgtctctt tatctgcaat caatttagga tagacaagtg tgccattgtt ggtaaaaaca
     1741 gaatacattg ctgcttgctg gataggagta attagaagtt cgccctgtcc atatccagta
     1801 tcagctagca agatatctga gttaaagcta tcttcattag aaatttgtgc tggattcata
     1861 ctaattggca aatcaaggtc ttcaccaaaa atgaatttat ctaagcctgt acgaaatttt
     1921 ttctcaccca ttttcaacgt ttcttgtgcc gcatatatat tatcggaata gatcaaagca
     1981 gtttttaagt ctacttgtga tacatcacta ctgacacgcg ttgttttaaa agatccccaa
     2041 gaactgtctt tttgccattt aagcccgttg atcgtcaaca cttcatttgg atcgatagtg
     2101 ccgttgtcga gaccgattgc tgctgtaatc attttaaacg tagatccagg agcataacct
     2161 gtcgcaaatc ggctgatgaa tggatgttca ggattttctt cataagcttt gtaatcttct
     2221 tgtgagatcc cgtttgtcat tttgtttgga tcatagcttg gagagctagc aagcgcaaga
     2281 agatcaccgg tttttggcgt tgtcgcaaca gttgatgcag cttttcctcc tagactgtca
     2341 aaagctattt tttgtgcctt cgcatcgatt gtcaatttga tatctttgcc attttgaact
     2401 tcatgttcga tcagaacttt tttctctaca ccgtctgcat ctgtaatgct taatttccca
     2461 cctgttgtcc cacgaagatc cttatcaaaa gccatctcca aacctgagcg accgattttt
     2521 ccattactgc ttaattctgg attcttatca atatcttctg ctgtgatatc tcccacgtaa
     2581 ccaatcagct gagcagctgc ttcacccaaa ggataatatc tgccgtctac ttcttgaatg
     2641 gtagctccag ctggaagttc tggcgttgct ccatcaatga ttttcaatgg gacaaagtaa
     2701 tcgggttgta cccagctttg tgaaatcgcc tgattgatag catcttctgt taagtcgaag
     2761 gaggaagcaa tcgctttgat attggctgtt ttttcgcctc catctccaag tttacttgga
     2821 acgactccta attgttttag tttgcctgtt gttgctaatg gttcaccatt tcgatctata
     2881 atattccctc ttgctgcttc ttgtgtggtc aggcttactt tgtcatttcc ttccatttct
     2941 ggaaaaacca agttgggttg ccagttgatc gtggtttgac catcatttct gtctaatgtt
     3001 cctttataag aaagatcttt caattcacct aatgaggtat tcatctttgc tttatatgaa
     3061 aaactgtaag tagaatcgtc ttttttatct acttttagat tcgatatctg aagtcctttg
     3121 acatcagcag caccgtatat attctggtat ttatctaaga tctctttttc agataatgca
     3181 cttttatttg ccgccttttt tgatgtcatt tctgcagctt tgttataatc tcccttgttc
     3241 aaagcttgga caaattgctc aactgtcttt tctccagctt ctactgcttg ggtttcttga
     3301 tagtgctggt agtaaaaata accgccaccc gcagctgcta ttaagatcac tgcgccggca
     3361 atataagcgc ctgttcgatt tttgccgtgc ttgtcacttc ttttcatttt ttcctccttc
     3421 tcttgcttgt cattccttta tttcactatt tatacctgtt tattctaaca tatgtctttg
     3481 aggaaatcca taccacaatc cagaagcaca ctttctggaa ggttcgtttg gatactacca
     3541 agtaatttcc caaccgcttt gggtagcttg aatattgaag ttacgtcttt cagctgctga
     3601 gagatagctt tgatcacttg ctgctgcctt ctgatcctcc cgaagtctcc ttctttgtct
     3661 tcccgaaatc gagcatactg gagtaaagta ttaccatcca tcacttgctc tcctttgttt
     3721 atatatacgc catctaaatc tagagatttt tctgcatcga tcttgactcc accaggaaac
     3781 agttcattaa cgcaatcgat aaaatcttga aatgtgatac ttgcataata gggagcttca
     3841 aatttgaaat tttcctttaa tgtttgattt aataaatcaa ctcccccata agagtaagct
     3901 gcatttatct tattcattcc gtaacctgga atatcgacat atgaatctct catgatagag
     3961 atcagttttg cttgttgtgt tttctggttg tattgtgcaa ccagaagtga atccgagcga
     4021 cctccaccaa tcgatctttt ttcaccgcat ctcctcctca cacagcaatc cacttgtcag
     4081 gtgaggagcg agtttgtctc atcaaataag tcagtcacaa aaatataaag aaaaggtcag
     4141 aaaccttttc gaacaagaat tctgacctag catcatctca tcgtccgagt tttagcacta
     4201 tacaacgtaa agaaaacatt cttagctatt ggaaaaagcc ttaatccaac agtttctgtt
     4261 ttacccattg gcgactttcg acgtttctgg gcaacagcct atgtttgtat aggagcctca
     4321 cctaacaagg ttattactat attctattta cgagttcatg atagactttt cctaatgatt
     4381 aggcaatgga atttgaaatg aaatagtgaa atttaacaat ctttaatatt ttttagaaaa
     4441 aaataacaaa taagttgaaa gaatcccgtt gcaaattctt tcagttgcac caggattctt
     4501 tgataatttt ttagttttat gtgttttttt atatgttttt tcacttttcg gaataaatat
     4561 ttatttgtta aagtaattaa acaatccgct aatcgtttga tctggcattt ctatttcctt
     4621 accatctaaa gtaacagtag taacgacaaa atggtcttgc aatggcggaa cttctaagta
     4681 ctgcccttgc agaaaatcat tcactgtttc atattctttt ttcatttctt ttgaatctgt
     4741 cgatggttta tagcttattt ttaacataac tagtgctcct ttttattcgg gacgtgcaat
     4801 cgtgaaaatt tctcctattg ctgcataatc atttcctgcc aaacggctta cagcattcaa
     4861 ttttcttgga tcgatccttc ctgtcttatc atcatagact gtttcattta aatgatattt
     4921 ctgtacttct agcaaaaata aatcagctgt tactttattt ttattccgta tctcgacatg
     4981 ttcatataag gatgtttcaa aacgaacttt tgcttctgtc aatgctggta ctgaaatgat
     5041 tactgaatca gttaaagtca agtcagtcag gtacagttcg ctttcctcat taggcagatt
     5101 ggctgctgtt ttatttactt cttctacgtt gtcctcatca aggatatgga taacagcttc
     5161 tcttgtctca agaatattct ttgctgtgtc ttttactgca tcttgttttc tctggatcgc
     5221 caaggataag atgggtcgat tcgaactgac gatattgaag taactgaagg gtgcgatatt
     5281 cactatatct tttgctgatt tagtcgttac tactgctact ggtctaggga tgactgaacc
     5341 aatcagcaac ttgtaatttt cacgttctgt caattctgaa ggactccgtt caaacatggt
     5401 ttcgccttct ttctataaaa ttcgttaaaa agcagatctt gaagacaaaa tgaaaagtaa
     5461 tgcttttttc atcttcttag aatctgcttt aaactatcta tcaatttttc ttttttgtca
     5521 agaatctgtg ccgtgcgtgt tttttagtta tttgctttac ttgcggcttc gacatatctt
     5581 ttagtgatca attgcggacg ggtgataata gatccaacta cgactgaaaa agcacctaat
     5641 tcatatgctt tctctacttg ttcaggagag tcagtatgtc cttccataat tactgggata
     5701 tcgacttgtt ctatgatttg attcaataaa tcaaaatcag gtgtctgcgt ctttcgtgaa
     5761 tattctgtat agcctgctaa agtcgtagac aaaagatcaa aaccatcttc ttgcgcttga
     5821 agcgcttcct caaaagtcga aatatctgcc atagctaacc gaccgtgttc gtgaatcatt
     5881 tggacaagtt cagcagttgt tttttctgtc ggacgtttac gagaagttgc atccagtgca
     5941 atgatttctg cttgcgtctc caaaagttct tccacttctt tttctgtcgc agtaataaac
     6001 acttctgacc catcgtaatc tcttttgata atccctatta ccggtaaatc aacgattcgc
     6061 ttgatttcat tgatatcttc tgtcgtattt gctcgaatac caactgctcc gccttctttt
     6121 gcagccaaag ccattctccc cataataaaa gaactgtgta gcggctcgtt ttccaaagct
     6181 tgacaagaaa cgacgactcc gcctttgatt tgttccagtg tatccacaat atctcctcca
     6241 gtaatgttat ataaaaatac agcttcatat aaaaatgtaa cccttttcta ttcaggagac
     6301 aataatgagc tatttctttc agcagacctt tcttactaaa agaaaattac ttcaaattct
     6361 tcgtatacga ccagcttctc ttctgtaaat tctatgttca acgcttcttt ataagcatgt
     6421 ttaaaaaact cagcatggac tttccgccaa taagctaatg tccgatcccc ttctccttct
     6481 ttccaagcat gctctttcgt gacttcacta aaaggcaaga tggtcacatt tatatttttg
     6541 atgatacaaa ctggttcatt ttttccattc aggaccacgt ctaattgacc aacttttggt
     6601 aaaggctcat tctcttgttt atacagatcg taaccacttg atgtaccagt ctttatcttt
     6661 tttacaacta gttcgctcaa ctcgtctgcc attttttctg tatccccaaa tgaaaagaaa
     6721 cctaattttt ctgattgata actaggatat ttttcttgga actttttgac atattcttct
     6781 acttgctgat acataacatc cacccctttg ctttttataa tgaaaaaacc atccagatac
     6841 tagtataaac aattatctgg atggtgagca taatgaatta ttgaatattc aagacacgca
     6901 gactatcttc aacgatttgt tttggtgtta aatgataacg ttcataaagc tgccctacag
     6961 gaacgctatc cgtgaattct ttctctgcac caaaatttaa tacgttcatt gaagaatcac
     7021 cataataacg actgatattt tcgccaaatc ctcctgacaa actaccatct tctaatgtga
     7081 tgactaattc gtgtgtttct tttaatttat ctaatgtttc tacgtcattt tctgtgatcg
     7141 ttcctggatt gatcaacgtt gcttcgattc ctttttttct aaaagctttt actgtttctt
     7201 ttcctaattc aagaaaaccg cctaaagcta aaattgctac ttttcctcca ttatttactg
     7261 tttgataggc tggaatacta taatcatttg cgatagctgt accatgttct acaccatgtg
     7321 taggaattcg gattgctaca ggctgcgctg tttgttcgaa cgcccaatct agcatagaga
     7381 tcatatcctc ttttgttgct ggagcaagat atttgatgtt cgggatactt gataagtaag
     7441 gaatgtcaaa tgttccttgg tgtgttcggt cactagctga gatcgttccg ccaccgacta
     7501 aaataatcac tggattctcg ttgatcgcta aatcatgtga taattgatca tatgcccgtt
     7561 gcaagaaagt acttgattgg aagacgactg gacgtactcc ctgagaagcc aaagcagaag
     7621 cgaaagtgat agagtgctgt tcagcgatcc ccacgtcaaa ataatgatct ggatgttttt
     7681 cttggaatgt tttcagtcca aaggcgcctg gtatcgctgc attgactgcg gcaataggag
     7741 ctgtacttgt ttgcattttc ttatccaaat aatccatgat gatcatatta aatgtttctc
     7801 cagaagttac ttgctttgcc tccccggtgg acagatcaaa tggtacatgc caatgatatt
     7861 tcattttatt atcgattgcc ggctgataac cgcgaccttt ttccgtatgg acgtgaagta
     7921 caatcggatg atcaatatct tttacttctt caaataaacg gatcaccgtt tccagatcgt
     7981 ttccattatc cacgtaacga taatcaaatc ccattgcttt aaataggtta ttgctgcttt
     8041 taccgttggt ttgacgaagc tcagctaagt ttttatataa actgccatga ttctcatcaa
     8101 tagacatctc attatcgttt actaatacga ttagattgga ttgtaaaaca ccaccattgt
     8161 tcaatccttc aaaagctaag ccaccactta gggagccgtc accgataaat gctaccacat
     8221 ttcctgattc tttcttcaga tctctcgctt tagctaaacc tgttgctaag ctgatagatg
     8281 tagatgtgtg ccccactgta aaatagtcat gtttactttc atgttggttg gaatatccgc
     8341 tcacttcacg gtaatgtcct ttttcaaaac cattttttcg accagttaaa agcttatgac
     8401 cataagactg atgggaaaca tcccaaacga ttttatcctt tggtgaatca aatacataat
     8461 gaaacgcaat cgttaattct actacaccta gattcggacc aacgtgccca cctacatcgc
     8521 tgtccttttg caagattttt tggcgcatct cttctgccaa ctgtttcatt tcctcaacag
     8581 ataatttttt gacatctgcg gggctattga ttgcatccaa tacatgtaat actgtactca
     8641 taatacattc tctcctccgt actatcttct tttaatgaaa atagatttgt tacactttat
     8701 ctattctact acttagagtt gactctaagt caatagcatt ttaaaaaaat ttcagagaaa
     8761 gcttgttata taagtagatc ttcgcacgaa tcaggactca aataaaaacc ttctacagac
     8821 aattaaaaag ctttgtagaa ggtctcctaa ttaaggtctc atcaagaaat atgttttttt
     8881 aataggtgaa gttcctgagc aatatgatac aaatggatgt ttgcaccttt taatgagatt
     8941 ggatagatct ttgtcttttc agtttctaat ccattttgat agctagcagc atcgcacaaa
     9001 accactcttt ttttcgcaca tagacattca tcaggcaatt cttctaatga acgaaataaa
     9061 ataggattta tttcagcgat gccttcttcc gcatacaaat gaaattgggt tcgataaaaa
     9121 aattgtttga ctgaactttc tgaaacaaca aaaatttcta tttcctgtct ccccgcttgt
     9181 tcactgttga tcaaagaaag acttttgacg aattttcgaa tgaaattttc attccatctt
     9241 agttggattt gatttttctt tttccactct tccaatattt ctacaatttt tccgtacaga
     9301 agttgttctt tttctgatag aagatagatc ctttccggta aaaatacttg tccatctgcc
     9361 cacaacgtac ggacaaattg taaaaaggat tgcttgaaaa taaaattttc tgcatcgtcg
     9421 atttcagcga tactaataat ctgtaaaatc aattcattca gtgataggtc attgagaaca
     9481 aaatttttat aaacaacttc gaaatctttt cttaacagct ccatgttctc attggtataa
     9541 tttctactgt tgaacaaaga aaaaatataa tagacatctt cttcttgtaa gtctagaatt
     9601 cctttttgct cttccaatcc ttgacagact aattgaaata acggcttcgt ttctgcgctt
     9661 ttcttatctt cttctttcag tccaagggga aatgcctttc ttccgattgc gatctctatt
     9721 cctcgtatga taaaggactt ttcatcttcc gaatatcttc ttttaaaaaa atgagtctct
     9781 acataatcaa tcaactcgtt acaagcttct tctacatacg gatttttatt ttcatagcct
     9841 tcccaattga cataacgtac taaagctagc aagatgttcc gcgagtcaaa ttctgggata
     9901 atgatttctt tatttttcaa ataatcattt tctttaaaaa aatccaatac gatttttttg
     9961 attgtatata ctttgctcaa agaaataaat tcaagatcag ccagttctgt actcgtgaat
    10021 ataccattaa aataataact taaaaacttt aaaaaataag attgaccgta cagaaactgt
    10081 gtgagctcag aaagagcaat accatgtttg aatacgagtt tatacttatg cttctctctt
    10141 acttgttctt ttatacaata ttctagacac tcggctagac tttctagatc gtgaaggatc
    10201 gttggtgtag tcacccttag agaaatagct gtttgttcga tgtctatttc ttggaattca
    10261 aataattgtt cacacagata cacttttcta aatatatttc gctcgatgta attttccagc
    10321 attcctgata cacgcctcca gtttttctta tgattttatt taatcatatc tgtaaatcgt
    10381 atttttgttt cctttttatt tagaaaagcc ctttcaaaaa ccaataaaaa aagaaactgg
    10441 gacaaaagtc aaacaagctc acaatttcag cctcattgta ggtagctttg agccgtttgg
    10501 acttttaccc cagcctcttt caagaatggt tattcaaaga tgtcagcgtc tagttctttg
    10561 actacatctt tcaacaattg gataccagct gcgctgttac caaatggatc tagtgccggg
    10621 ctgaagacac ctatgccgta acgatttggc acagctgcca tcaacccgcc gcctacaccg
    10681 ctttttgcag gtacaccaac atggacggaa aactctcctg attcatcata caaaccagca
    10741 gttgtcataa ttgatttgac gattgtagca ctttcttcag taatgatttg tttgccattc
    10801 caaggagcaa ttcctttatt tgccagcaca gcagctaatt ttgctatgcc tgtagcggtg
    10861 acattgaggg agcattgtct gaaataagca tcgagtatct ccggtacgtc tccttcgatc
    10921 atttgattac ctttcatata gtaagcaaga gagcgattga tatcacctgt tcttgattca
    10981 gaatgataga tttcttcatt caatgtgata ttaggatcat cacagatctc tttcataaat
    11041 gacaagattc gctcaaattt ttcatctgca tctttaccgg caatcaacga ggttgttgca
    11101 atcgctcctg cattaacaaa aggattcatt ggatgtttgc gatgattgat ttccatattc
    11161 atgatagaat tgaaggcaaa accggttggt tccgtgttga ttgtttgaaa gactttttct
    11221 attccgttgt ctttgattgc taataacaat acgggaactt ttgacatgct ttcgatggca
    11281 aagcgcacat cggcgtctcc tccctcaatc agttgttttg tatccaaatc ataaatcgcc
    11341 atacctaatt gtttgggatc cacttcagct aatgctggaa tgtaatccgc cacttgtcct
    11401 gtttgataat agtggaaatt ttttccaatc aattgttcta attcttttac ttccatttga
    11461 ataaccagct ttcatttttt atttttctgc atgctggaat ttatgacggc atgcgtagat
    11521 cgtaaatgga ataactaaga cgataagata agaaacaatc agaatcgttt cataaatccc
    11581 atgctgtcca gctgcgatac tagcaggtgg aacaaatgag atacaaaaag caaaaagtga
    11641 agtcaaaaga cctaaacaag caacgattac ttttacaact tttccgcctg gaacctgata
    11701 ggctctttca agactgtcat ctttaaaaac cagcttcaaa tagcccgcga aaaacataaa
    11761 gtacaacatc aggtagatta caacagtcaa tgacatcgct gcaagaaatg aaagattatt
    11821 ccccccgccg cttaatgtaa agacggctgc ccagattgtt gcaacgatcc catttgcgat
    11881 catcaaatga gcaggaacac cgtgtgtgtt ttcttttgca aaatattcag gaagtaatcc
    11941 ttcttctgct gctacgtgaa gtgcttttga aggagaggtt acccaagaag caatttctcc
    12001 gatgacacct aaacaaacaa gaatcgcaag aattttcgca atccatgcaa gatttggatc
    12061 aatgtgtctt aataggaatt ctaacgattg aatcacccct gtattaagag acaaaccatg
    12121 ttgaggtact gtagtagcga cggtcaatcc gccaaaagta tctaaaatga ttgccagaat
    12181 aaccaataca aacattgcta atggataatc ctttttaggg ttttccattt catttgcatg
    12241 actggcagat gtttctacgc ccatatatgc taaaataaag gaaacaaaaa caaccagcgt
    12301 aggcaatttc gaaaaatcag gaatcaatgt tttcatagac agtatcgttt gtagtggagc
    12361 gcctgaagcg acataatgga tccctaagcc aaacaagact aatgctggca acaagatacc
    12421 agctacaaaa ccgatccgag caatcaatga agtgtatttg gttcctttta actgtgagag
    12481 taaaatagcc cagaaaataa tcaataggat tgccaatttc agatatttat tgttttgtat
    12541 agcaccaata cccgttgcat aggataatgc accagttaag aaatacagca tggtcaagta
    12601 gccaactgtt atctcaaacc attggaaaaa gattgcggca aaaccgaatc gttttcccaa
    12661 tgtgcgactg acccaagtat agacaccgcc tttttcccat cctttcaccg ttgccatctc
    12721 tgctgcgcac aatgccacag ggataaacca tagcaaaccg cctaataaca agaaaaatac
    12781 taagctgaag cctgaggtcg cgaatgtggg atattcatat aatgaaacga ccattgagat
    12841 ggtcatagcc aaaaagccaa agaacgttaa tttttttgca ggaattttcg ttttgctttg
    12901 ttgcatgaat cttcctcttt tctatttgaa tttgtttcaa ttgagtttgt tgccaaagaa
    12961 gaaaaagtcc aatccttctt cttgttcttc ttttgtagta gtgatcgttg taaagtgcat
    13021 cgccgatttt aaaatcactg ttgtcagcac ataggcatac acggcagtaa tgatgacacc
    13081 aatcacttga atccctaaga agtaccagct gcttggattc gttagtaaat ggctgttaga
    13141 tgcaaagatc cctatcagaa tcgtacctgt gaatccgccc atgccatgaa caccccacac
    13201 atcaagtgca tcatcccatt gtttcttttt acgaaaatca acagcagcat gacagacgat
    13261 acccgcaatg atcccaatca gcatggcact ttttgcttct acataaccgg cacaaggcgt
    13321 aatggctgcc aatccagcaa cggatcctgt tagtacgtcg acaaaatcaa agccatttcc
    13381 ttttactttt gcaatgatca gccaagtaat catcgcaaat gcaagagcga tgaatgttga
    13441 accaaatgca ttgactgctt gttcatctgc tcgaagtgca ccaccggaat taaaaccgaa
    13501 ccagccaaac caaagcagtc ctgtaccgat agcagctgcc ataagactgc tatgctgact
    13561 tctttctcct aaaggaatct ttctttgacc aagtgtgagg atacatgcta aagagccgaa
    13621 tccagctgtc gtgtggatga ctgctccacc agcgaaatct ctaaatccca tctgatccaa
    13681 aaatccgcca ccccatatcc agtggcaaac agggaaataa atcagcagat tccaaaaaat
    13741 aacaagtata atatagcctt ttaaattcaa tctccctgca aaagctcctg tcatcaaagg
    13801 aacggtaatc acgataaaca tcagttggta aagaaagaac attaaaaatg gaatcgttgc
    13861 accatgaaac atgttgggga agcttgtaac atgtctcatc aaaaagaaat ctgccgggtt
    13921 tccaataaca ccgccgatat cccgtccaaa ggccaatccg aatccaccaa atacccacat
    13981 aatcgttgtt atcccaacgg agagaaaaga ttgagacatg atcgttagta tattattctt
    14041 ccgtgctaat ccgccgtaaa aaaaagctag accaggagtc atcaggcaaa ccattgctgt
    14101 acataaaatc atgaatgctg tatctcctgt attgataggt aatgtattca tttttttcgc
    14161 ctcctttcta cgattacgag tataaaagaa ggtgtagttg tttttttctt ctgttttttt
    14221 gaattaatac ttgcatttat aaaagacaga caattaataa aagcttgatg tgttgtgctc
    14281 tttttcgttt ttcacacaaa taaagacaaa aaataaacgg tcttcttttt atagaaaacc
    14341 gtttattttc gtcttttttt atttttaaag atattttttc ataaggaata aagaatactt
    14401 catataaaat atataaaaaa gaaccgcagc gagtgtcgct acggttctca aatttgttct
    14461 tcatttcaac ataagttttt cttcaatttg cttataattt acgtgcaaag aatgaaacga
    14521 ctaatactaa aatgattgca ccgatcaatg atggaatgat agccattcct gcaattgttg
    14581 gtccccaagt tcctagtaaa gcttgtccaa ttgctgaacc tactaaacca gcaatgatgt
    14641 ttgcgatgat ccccattgat gatcctttat tcgtgattgc tcctgcaatt gctccaatga
    14701 taccgccgac gattaatgcc caaataaatc ccattttaaa ttcctcctaa ctgacttctt
    14761 acttcttatt gtacacgtgg ttcgttattt tctttgactt tttgtgcacc tttgcctaca
    14821 gcagattttg ctttatctgt ttggcgtgaa gcaaattgtc ctgtggattt tgctgcttct
    14881 gttactttgt cttgtactgt ttcttgatct ttttggtatt cttcttgtgt tttgatgtct
    14941 gcaacattca cattcacttc gataacatct aaatgtgtca tattgtttac ttcagtactg
    15001 atcaattctt tgatttggtt gtaaatatct tcgatgtctt taccgtattc tacgatcaca
    15061 tctagatcca cagctacttg tttcttacct acttctgtat tgatacctgc tgttacgtta
    15121 tctgtgttga ctaatttttc agccatattt gagaaaaatc cgccatctaa tgtcaataga
    15181 ccatctacat ctttcaatgc ataaccgatg attttttgga tgactttgtc ttcataagtc
    15241 aattcaccat tcatcatgcg gttttctcct ttttcatttg ccaaaactga tccatttgtt
    15301 gtattcatgt ttttgttttc catctttgtt tcctcctatg atttaaaaaa ttgatcaact
    15361 attcctgact gtttcaaata aaaaccgatt cctgcgccaa ttgctgtcat gattatgatc
    15421 aacaatgtct tgaaaaatcc aatcgttaac cataaaacag ctagcagtag tccaagaccc
    15481 ccaaagataa tagggagctt gtatgctgaa aatagttctt tcattatttg gccctcctat
    15541 tccactcgag agtgcttgta gtctcttgta tcttcttgtt cttcataacc gctgtatgtg
    15601 actgccactt tgaccgtttc ttttgttccg agtactttag ttacttcttc ttcgatatct
    15661 tgcatcaata cacctgtttt ccctactaat gaagaagttc gtttcaagtc tccgttgaca
    15721 tggatatgga ttcgattttt cgttgaacgt atccgtactt taggtgaatg aatgaactct
    15781 tcttcgtgta aatgggaacg taccaaccct tcgatcgctt ttttgtcgat tgttagtttg
    15841 ccatcttccc gtttcataac gaaagttcct cttgactttg gatagaaaag aacgactagc
    15901 aagatgataa tagcaagaac ggcaaatact acagctgccc agaaaatata ttgttgcatg
    15961 tagtaattcg ttaaagtaaa gaagcgaaat cgttccaatt gttcaggcat aatatacggc
    16021 gcattgatca acgcaatcgc tagaaatact gaaagtagca gtaaactcat gataacaccg
    16081 attattttta accccttatt cattttccac ctcctataaa cttattttta ccctgtaaat
    16141 aactctaccg acaatcacag tatagatagc gaacagttag catagcatca gtaaataaag
    16201 cgctaccaaa cattgctgga tcataacggc gttttattgc cagtctgata ctgaaataac
    16261 aattgcttac ttattctcta tttacttttg attgctggct atacttaaag gatattattc
    16321 aagattaaaa attgcaaaga ttatgctcag ttattattac aagattctat ttatt
//
LOCUS       DACESX010000055        16236 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.055, whole genome shotgun sequence.
ACCESSION   DACESX010000055 DACESX010000000
VERSION     DACESX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.055"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(65..1000)
                     /gene="murB"
                     /locus_tag="IXF88_001500"
     CDS             complement(65..1000)
                     /gene="murB"
                     /locus_tag="IXF88_001500"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1508238.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1073..1993)
                     /locus_tag="IXF88_001501"
     CDS             complement(1073..1993)
                     /locus_tag="IXF88_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1508239.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2106..2858
                     /gene="xth"
                     /locus_tag="IXF88_001502"
     CDS             2106..2858
                     /gene="xth"
                     /locus_tag="IXF88_001502"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1508240.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3722..4255
                     /locus_tag="IXF88_001503"
     CDS             3722..4255
                     /locus_tag="IXF88_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ1508241.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4745..5269)
                     /locus_tag="IXF88_001504"
     CDS             complement(4745..5269)
                     /locus_tag="IXF88_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1508242.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5262..5735)
                     /gene="tsaE"
                     /locus_tag="IXF88_001505"
     CDS             complement(5262..5735)
                     /gene="tsaE"
                     /locus_tag="IXF88_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1508243.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5858..6838)
                     /gene="pta"
                     /locus_tag="IXF88_001506"
     CDS             complement(5858..6838)
                     /gene="pta"
                     /locus_tag="IXF88_001506"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1508244.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6991..7671)
                     /locus_tag="IXF88_001507"
     CDS             complement(6991..7671)
                     /locus_tag="IXF88_001507"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1508245.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7797..8666
                     /locus_tag="IXF88_001508"
     CDS             7797..8666
                     /locus_tag="IXF88_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1508246.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8692..9207
                     /locus_tag="IXF88_001509"
     CDS             8692..9207
                     /locus_tag="IXF88_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508247.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9254..9817)
                     /locus_tag="IXF88_001510"
     CDS             complement(9254..9817)
                     /locus_tag="IXF88_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1508248.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9872..10051)
                     /locus_tag="IXF88_001511"
     CDS             complement(9872..10051)
                     /locus_tag="IXF88_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508249.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10128..10637)
                     /locus_tag="IXF88_001512"
     CDS             complement(10128..10637)
                     /locus_tag="IXF88_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508250.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10733..11113)
                     /locus_tag="IXF88_001513"
     CDS             complement(10733..11113)
                     /locus_tag="IXF88_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1508251.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11443..12192
                     /locus_tag="IXF88_001514"
     CDS             11443..12192
                     /locus_tag="IXF88_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1508252.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12376..13653)
                     /locus_tag="IXF88_001515"
     CDS             complement(12376..13653)
                     /locus_tag="IXF88_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508253.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13732..14376)
                     /gene="pcp"
                     /locus_tag="IXF88_001516"
     CDS             complement(13732..14376)
                     /gene="pcp"
                     /locus_tag="IXF88_001516"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1508254.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14575..15519)
                     /locus_tag="IXF88_001517"
     CDS             complement(14575..15519)
                     /locus_tag="IXF88_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1508255.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15521..16207)
                     /locus_tag="IXF88_001518"
     CDS             complement(15521..16207)
                     /locus_tag="IXF88_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1508256.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4672 a         3372 c         2873 g         5319 t
ORIGIN      
        1 accacaaaaa agagcaggac ggccaccgtc ctgctctttt acttatttta agttccatta
       61 cttattaatt ggctaaactt ctgcttgttt ctgatattgc ttgtttttct ccaatgatcc
      121 gcacttctgt ttctaaagac acatcaaatt tttctttgat tacttcttgg atgtgcgcaa
      181 tcaattcaat ataatctgtc gctgtcgcat gatcgatatt gacgatgaat ccagcatgtt
      241 tttcagagac ttgtgcaccg ccccatttca ggccttgcaa tcctgcttgt tggatcagct
      301 gacctgtata gtgtcctact gggcgcttaa agacacttcc gcatgaaggg tattctaatg
      361 gctgttttga ttgacgcaat tcggtcaact catccattct tgctttgatt gcttcataat
      421 cccctgtttg caaactgaat cgagcttcca atacgatagc acgtaattct tggactttac
      481 tgtgacgata agaaaatacc atttcgtctt ttgtcaaagt tttcaatgtt ccatcttcaa
      541 gcaaaagatc cacttcagca aaaacatctt tgatctctcc gccatacgca cctgcattca
      601 tataaaccgc tccaccgaca cttcccggga tgccgcaagc aaattcaaat ccagtcaatg
      661 attctgccaa agctgcataa gtcgtatcaa tcagtgaggc acctgcttcc gcaatgacca
      721 tcgttccttt gacatgaatc tgtttcattt cagtcagcat gatgactaca ccgcgaatcc
      781 cgccatcttg aacgatcaga ttgcttgcat ttcctaatac catccaaggt atatcatgga
      841 ttcggcaata atcgatgatt tgctttactt cttcttgttt tttcgggaat gcaaggacat
      901 cagctggtcc acctgttttc gtaaacgtat agttcatcaa tggttcatca aacaatatag
      961 taatcgtggc taagctttca actatttctt ctttattcac tgaggatttt cctttctttt
     1021 ctcaaacgtc ctcattattc tatcacgctt aatacaatct tgccagtctt gttcaaagtt
     1081 gatgacggta gtgatcttta agcacttcct cacataattc atgacttctt acaacttctt
     1141 tttgtttaag caattctgtt tccgatttac ctaaacggat ctgagaaaag aaggcctcta
     1201 ccattggctc aaacccgcgt ttgactagcg tgtttgtcca gtcgccaaat tctttcactt
     1261 tttttccatc agcatcttga atgacaagct gggtcagatc gtttacttca taagtaccat
     1321 ttttacttgt tacttgatat ttttctatat tagcgccact tgctaaatcc atcgttaaaa
     1381 tagccgtttg attttgtgtt tctaattgta aaatggcata ttccaaaaat tcctttgttt
     1441 ctcttatctg gtattttgtt ctcaaaatgg gttcatctaa taaatacaca gctgtatcaa
     1501 taagatgtaa aaacaaatca taaaggacga actctgtcgt ttcttttgcc gctattcgat
     1561 ttttttgcag gtggatcaac cgcttatctg ggatacgttt caattcttct accataggag
     1621 caaagcgccg gttgaaaccg atcatcaaaa ttatttggtt cttatcagct aattcttgca
     1681 attcacgaac ttcatttaga tggacactca gtggtttgtc aatataaaca tggataccat
     1741 tttctagaca ttttttggct aattcataat gtacttttgt tgctgcgtgg atcatacatg
     1801 cttcgatttt ttcttctaaa agctcatcga tcgtttcata tagatgttgg aacccgtact
     1861 ctttttgaat tctttctttt gtttctttat ttcttgttgc aaaataaaaa tcaagtgttc
     1921 cttgtttttt cgcataagtt ggtaaatatg ctttttctgc gatattccct acaccgatta
     1981 caccaatctt catctacttt cctcctggat catttagtta ctttcatctt acaccatttt
     2041 ttactaaaat gaggtacaat agtatcgcaa aggtaaggaa attaattcga aggagtgtgc
     2101 ttttattgaa atttatctct tggaacgtaa acgggcttcg tgccatcgta aataaaaatt
     2161 ttttagaagt ttttcatgag ttagacgccg attttttctg tctgcaggaa acgaaactcc
     2221 aagcaggaca aattgattta gatcttccag gttattatca atattggaat tacgcggaac
     2281 gaaaaggcta ttccggcaca gcgatttttg ctaaaaaacc agcattgaat gccacttacg
     2341 gaatgggtat cgatatccat gatacagaag gccgtctgat tactttggaa tattccgatt
     2401 tctttcttgt aacctgttac acgccaaatt ctcaaagcga attaaaacgt ttagattacc
     2461 gattagaatg ggaagaagcc ttttataatt atttggaaaa tctaaaaaaa caaaaaccag
     2521 tcatcgtgtg tggggatcta aatgtcgctc atcaaaagat tgatttgaaa aactggaaaa
     2581 cgaatcaaaa aaatgctgga ttcactccag aagaaagagc ggctctttct cgactgttgg
     2641 ataacgggtt tatcgataca ttccgttatt tttatccaac ccaggaaggt gtctactctt
     2701 ggtggaacta ccgattcaat tcaaggaaaa acaatgctgg ttggcggatc gattactttt
     2761 tgacgagcaa agatttagag ccaggactag ctgatgcaaa gatccatacg gacattatgg
     2821 gcagtgatca ttgtccagta gaattagatt tgaaataaag aggttgtgaa aaagtcgttt
     2881 agtttcgagt attaagaaaa attttgtgaa aatggctttc cacattttgc ataaatttgg
     2941 cataataccg agaaactggc ttttgaacac tgtttatcga gagattgtga aaaagctgtc
     3001 ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatattttt cgtttggttg
     3061 ggcttattac cgcagataca agcttttgaa caccgtttat taagaggttg tgaaaaagct
     3121 gcactgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt tttcgtttgg
     3181 ttgggcttat taccgcagat gcaagctttt gaacaccgtt tattaagagg ttgtgaaaaa
     3241 gctgcactgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat atttttcgtt
     3301 tggttggact tattaccgca gatgcaagct tttgaacacc gtttattgag aagttgtgaa
     3361 atcagcgtcc tgacctgagt attagagaaa caaatggcaa aatagttcct catattttgc
     3421 tattttttga gctaataccg agggtcagct gattgaacac cgtttagtgg agaagttgtg
     3481 aaaaagtcgc ctactttcga gtcgtaagaa atttttttga aaatggttcc tcacaacttt
     3541 gcataaattt gacttaatac cgagaagtca tgatccccat ctaactccaa atcacaagga
     3601 atgagttcaa aaactagctt ttctcaacaa atattacata aaaaattcta tgttttttgt
     3661 taaatttatt tgcttttttc aaaagtatgt tacagtaatt tcccaaggag gttttacaga
     3721 aatgaatttt gttgccatgg attttgaaac tgccaatcat cagccttata gtgcttgttc
     3781 tcttgcgcta gtcatggtaa aaaatagcca gatcgttgat gaattctata cattgataca
     3841 gcctgagact ccttttttct ggcgaaatgt ccaaattcac ggaatccatc aagaagacgt
     3901 acgcaatgcg ccgaaattcc cagaagtctg gcaaactatc caaccatatt ttcaaaaaaa
     3961 tcgtttagtc gttgcacata atgctgcttt tgatacaaag gtcttagcgg gatgcttaga
     4021 ttattatcat cttgaacagc ctaattatct ttctttatgt acagtgaaaa gtagccgtcg
     4081 cctgtttcct gaatttccaa atcatcgatt gaacactgtg tgcgaaaatt tgaacatcca
     4141 attagatcat caccatgatg cattggaaga tagtcgtgca tgtgcggaaa tcctgcttta
     4201 tcaagaaaaa aaattcggga cagatccatt gaaaaaactt gtgactataa agtaaaagaa
     4261 tacttatgaa acacacaaag aggtcgtgag cctgccgttt cactccgagt cacaaggagc
     4321 aatttcaaaa aatagcttct catgtttatt aaatattgtg actggtgacc gaggagctgg
     4381 caggagaaca ccgtttattg gagaggttgt gaaaaagctg ttctgcatca agtaataaga
     4441 acagccaaac aaaaatagct tctcatattt ttcatttggt tggacttatt accgcagatg
     4501 caagcttttg gacaccgttt accaagaggt tgtgaaatca gcgttttgac ctgagtatta
     4561 gagaaacaaa cggcaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaagg
     4621 tcagctgatt gaacaccgtt tattcaaaaa aaatggactg cgacacgact tttgtcacag
     4681 tcccttcgtg tgtttttctg ccttctcaaa aggatgacag tttctcactc ttttcctatt
     4741 tcttctattc aatcaaataa ctcattttat atacatctaa aaattcacct agatctgttc
     4801 ttgcccctct aggcatgacc gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg
     4861 ctctttcatt acgaacttga acgtcaagct ccaatcgaaa aagtacaccc atttccttcg
     4921 cccaactgat gatttcttct agcataagag tgcctagtcc cattccccaa tattcctgca
     4981 gaatactgat accgacttct cccacgtgag acaaacgaaa ttgactgtct gccttgacag
     5041 atgctgttcc aataatcgtc ccttcataga ttgcaagcag caataaatta ttattgcttt
     5101 cgtatatata atccaactct tcttctaagg tttctggtgg taacaggagt tctgcttcat
     5161 ccaaaactaa aaattctgtc tccttattaa cttgtcccat catcaaaaga atgtcttttg
     5221 catctcctgg cactgcttcc cgcaagatga tcccaacttc ttcactcatg attttcagcc
     5281 catttttcta gaattcgctg tttgaaggtt tcactttgct ccccgtatga gattagtttc
     5341 acataccgct tttctaaatc tgtatcactt ttttctaaaa tcagttccaa ataatcctcc
     5401 ggcagtgctt cttctaatag atttccccat tcaacaacgg aaagcccatc accttcaaaa
     5461 tattcatcta accctaaatc tgctccgcta gctgctaccc gataaatatc catatgatac
     5521 aacggaatcc gaccttgact atattctcta ataatcgtgt atgtcggact tttgatcatt
     5581 tgcgttatcc ctaatccttg ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga
     5641 tcaccagtca atatcagatt gtcacctggc attgcggaat gaccaattgc ctctgcgaat
     5701 tgttctgtca ttgttaatcc tgatagttcg atcatttttc atcttctccc gctcttcttt
     5761 ctttttgcca gtatatcgcg taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa
     5821 cagcagctgc tgtttttggc tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg
     5881 ctgtgataat cgataattta tatacatctt cttcatttgc tccacgagac aaatcagaaa
     5941 ccggtttgtt caagccttgt agaattggtc cgattgcttc aaagttaccg aaacgctgag
     6001 caattttata gccgatattt cctgactgca attctggaaa tacaaagact gttgcttgac
     6061 cagctacttc tgaatctggc gctttcagtt gtgcaactga aggaacataa gaagcatcaa
     6121 attgcaattc gccgtcgatc atgtattgtg gcgctagttt tttagcgatt ttagttgctt
     6181 caactacttt gtctacttct ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg
     6241 ctacttttgg ttcgatatcg aacaattctg ctgtttttgc agaatctacg gcgatttcag
     6301 ccaattcttg cgcagttgga ttcacgttga tcgcacaatc cgaaaagaca tatttttctt
     6361 gatctcttcc acgtaccatg atcatcgctc cgcttgtccg actgatgcca ggctttgttt
     6421 tgatgatctg aagtgctggg cgaacagtgt cgccagtcga atggatcgct ccagagacca
     6481 ttccgtctgc aatccccata tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa
     6541 tcttttctgc ttgttctttg gatgcctttc catttctacg ttcaacaaaa gtatccacca
     6601 tttcatccca cttatcgtaa ttatcagggc tgatgatcgt aaagctagat gttttgattc
     6661 ctctggcatg cgctgcattt cttatttctt cttcgttccc tactaaaatg ggttccacaa
     6721 gttcttctgc tttcaaccgg gcagctgcgc ctaaaatacg agcatcagta gcttccggga
     6781 aaacgatttt gatcccacgg cgaacaattt taaatcttaa actatcaaac aattccacaa
     6841 gatgacctcc agacgttttt atatgtatta tcatacacta ttccagaaaa aaagaacagt
     6901 ttattttatc tgtatcagtc aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga
     6961 actgcactat aacaggaaag cgctgtttta ttacactgtt tcaggcagtt gccaatcaat
     7021 cggttcttct cctaacgcaa ggagcgcatc attcgttttc gagaagggtt tggaaccaaa
     7081 aaagccacga cttgcagata acggactggg atgtggagat gttatgatga tatggcgact
     7141 cttatcgatc attttgattt tttcttgtgc cggttttccc cacaagataa acacgattgg
     7201 tttttcccgt tcatttaatt tttcaatgat cgtgtctgtt aatctttccc aacctttccc
     7261 gcgatgagaa tacgcttgcc cttcccgaac ggtaagaacg gtgttgagta aaaggactcc
     7321 ctgtttagcc catgacacta gatttccatg ttgaacaggt gcgattccaa gatcatcata
     7381 aagctcttta tagatatttc ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa
     7441 agataagcca tgagcttgat tttctccatg ataaggatct tgtcctaaaa tcaccacttt
     7501 aacttcttca taaggagtca attccaatgc ttcatagata tgatacatat ctggatggat
     7561 cttttgggac gcatattctt tttttaaaaa ttctcttaga tgcagataat aatcttttga
     7621 aaattctgag gacaaaactt cttgccaact gttatgaatg atttctttca aaaactccac
     7681 ctcctaataa aaagaataac tcacattttc ttcaatcaca agggacaatc ttcaccctca
     7741 gcacttgatt aatatggtaa actaggaaca agaagcagaa aggaacgata atcattatga
     7801 tcaaattaat tgcatcagat atggacggca cattattaga tgcccatatg agtgtttctc
     7861 ctgaaaatgc cgaggcgata cgttttgcaa atgatgccgg tgttgagttc atggtcgcta
     7921 ctggtcgaaa ctatcaagaa gctcgcgcag ctttagatga ggtaggaatc gattgtgcga
     7981 tgatcacttt gaacggggca caggtatttg ataaagaagg aaattcattg tttaccgtgt
     8041 cgattccaaa taatcaagca atctctgttt tagatattct agatgctaac ggtatttact
     8101 atgaagtagc aaccaacgat ggtctttatt ctgaaaatca agcgaaacga attgaaagtt
     8161 ttgcttccat ggtggctacc catttacctc accttacgta taaaatggcg attgccatgg
     8221 cctcagctaa gctggagtta ttgcacatca catacgtgga cagtattcgt gctattttgg
     8281 aagaaaagaa tctggaagtt ttaaaaatta tttgttttca tacagaaggg ccagctgtat
     8341 taggtccagt tggaaagcag atttctcttt taggcgatct agccgttact tcctctggtc
     8401 aaaacaatat cgaagtgaac cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg
     8461 ctcacgagcg tgggatttct ttagaagaag tcatgacgat tggcgataac ttcaatgatg
     8521 tcagcatgct gcaagtagca ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa
     8581 aagattatgc caaatataca acggacacaa accttgaatc tggcgtagga aaagccatat
     8641 tgcgcgcgat tgcagaagac ttgtagtttt atctagaaaa gagggatata gatgtcagaa
     8701 tttttcattc aggaaaagca gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa
     8761 ggacattccc tgtttttgat ggttggtcgc tggggaacaa agggcgatgc attgtctctt
     8821 tatgccatga atggcgatct cgttgctcac atcaagcaga taagcctgac attcggtacg
     8881 cgttttgagc tgtataaaga gttcgaaaag gtgggaacca tgcagaaaat attcaattgg
     8941 cccggtgact tttattatat tcgtagactt cactggacag tccaaggtga tatctataat
     9001 catcaatatc aaatccatca tttcaatcag caaatcatga aaatggataa agcaacactt
     9061 ttttcaggag attactatgt attggatgtc tcaaatgatg aagatgctcc agtttgtatt
     9121 tgtattgctg cggttttaga ttactggctc taccgtaaag acaaaagtcg cgataagcct
     9181 gtttcttacc gacttaactt aagttaaaaa gatgtacctg aaatgcggaa atacgcattt
     9241 caggtacatc tttttagata ttcttttgca tatgatcata aagatgacca ctgatcatcc
     9301 gctgaccgac aactttgaag tcatggcgtt cgtataattt tttagccttt ggattttttt
     9361 catccacact tagaccgatg acggagcgat tagctttctt cgccaattta ggtaatgctt
     9421 ctaataatcg cgaaccgatg ccttgtcctc taaaatcttc tgatacactg atagagtcca
     9481 aataccattc gttgggaaac gtttctttat caatgaacaa acgtgtagac gcatctagct
     9541 gatatttctc taagataggt accagtggct gatcgatgat tgattcttct tcatctgtat
     9601 aaccgtaagc aactcctgca actttaccgt ctatttcttc tacgatacct cgtgaataac
     9661 tgtaacgata atttggatca gtgattgctt cttctaaaat ctctaaagtc ttttgttccc
     9721 ctgtctgctt caaaaatggc agttccatat cttttagaat cactaagatc aatggggcga
     9781 tttctggagc atcttctttt ctcgcaaaac gaatcatagt ttcctctcct tttctccatt
     9841 ctaacatgca gaaaaagaaa tcgcaaaatc gttattttaa aaaatcttca atcttttcag
     9901 taatgaaata accatgagta acgattcttg ccgcttcagt tcggcaataa gggcagacga
     9961 ctggcatttc tttatcatta tgttcgacta cattattgac ttcttcgtat gcaaattttt
    10021 ttccgcaatt agggcatttc attttttcca tgttatctca acttcctttc atctttactc
    10081 tcttagctta ctcttatatt gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc
    10141 catacctgac ggtcctgttc tccatctatt aatcggactt tataataagg acgttcatct
    10201 ttgactagaa gtgtccactc tttcactttt gcttttttcc cgtagtagct gactgcttcc
    10261 ttcatcgctg cttgcggttg tttgaactct tccgtttttt ctatatcaaa aagatgttct
    10321 gcggcctttt ccgtgtcatt tcgtacggtt gttttcccag tggatggatt gactgctatg
    10381 acttgtcctt cacaaaaaaa ctggtattca tatgttgcat tcgtaggatt ctccaaatct
    10441 ttttcaactg caaaatgaat agcagatatg ttatgttctt ttgtttcgtt ttttacgatt
    10501 gctgctgctt ttttcaatga gatccgatat tcctggtcac taatatcatc catgtgaaaa
    10561 gcggcattga gcagtcccgt cataccggta atccctatta cgatgagggc agcaacacca
    10621 aacatcattt ttttcattct tcattctcct gtttcttttt tttaaagaat atcaagaaat
    10681 gaacgaatga aaaatcgaat gaaacgaaaa aaaagtgaca attttgttat ctttattcgt
    10741 gcagttctaa tggcaatccg tctggatcaa agaaaaacgt catttttttt cctgtaaagg
    10801 tatcttctct aatcggttct gattcgatcc ctaaggactg caattctgca attgtttctt
    10861 caatatcgtc tactttgaaa gcaagatgac gcaagccaca agcttctgga tagtttggtc
    10921 gttgtggcgc attagcaatt ccaaaaatct ccaattcagc acttcctagt ttcagatcta
    10981 atttatagtc atctcgttct ttgcggtaat tttctctgat tacttcaaat cctaatttat
    11041 taacataaaa atcacgtgat ttctgataat ctgaaacaat gatggctata tgatggatcg
    11101 ttgtaaggtt catatgagca ctcctattct tcgtttaagc tcggatctat attaatctcc
    11161 agttccattg taaatgtgtc agattgaaaa aaatagctgt ttttcctttt tataagaaaa
    11221 aaggcaaaaa aaacagaaag taggtcctcc aagacactct tcctgttccc aatataataa
    11281 atgaaagtaa gaaattatat tttcaagaaa acaaacagaa aattatcata acacaaatta
    11341 tttataattg aaagactagt tttttttcaa gaaaagcata caatagattg tgttagtaaa
    11401 tacagaacac actacattta gtgtcttaag gagtcctcat acatgaagtc atattttcag
    11461 tttttagctc atcagctaaa aggctggccg caacaaaatt attatctttt ctttttcagt
    11521 cttggctgcc aggtcatgac tcttgtcaat agtcccatca cttggctgtc cgttattaca
    11581 tttatcggta ccacgcttgg tgtattgtgt attttatcca tcaatgcggc aaaatcagtc
    11641 aacggtattt taggtatttt gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag
    11701 aactatttaa gtatcggtga acagattgct tatatgatca cattggatat ccccgtattg
    11761 ttaagtaaag aatggaatca taatatggct tcaaaaatcc gaaaattcac tggaaaaagc
    11821 tggtttatcg caatcatcag tactttcctt gtctatcttg tttcaggatt cctgattggt
    11881 cgttatacag atgatcctcg gccgtgggta gatgccattg ctttttctat tagcttgact
    11941 gctggtatca tcagtttctt gcgttacaac aaccaatatt actggtggtt agcttcaggg
    12001 cttgctcaaa tggtcctttg gtatatttca tttacgcatg gatctgcaag tttggcgatg
    12061 ttcgtgaaca gctctgttta cttgctgaat gatgtccttg catttaccgt ttcaccttgg
    12121 tataaccaaa aagaacgtga acgtatgcaa aaaatggaag aagaatacta caacgcacaa
    12181 caacacgcat aaaaaaactg agcgtgggac aaaagtaaag aacacttttg tctcacgctc
    12241 taaacacgta taaacggcgg aatcagaagc aactccttcg gaaataagct gaaattcaca
    12301 aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc ttggagttaa acgcttctgt
    12361 cccagcctct attttttata gtaattcctt cagatacttt cttgcctcat cattgaaacg
    12421 cagtgattct tctaacttct gataatattc caacgcttgt ttctgagctt cagtaaaacg
    12481 ataatgtttt cctttccatt tgtcgattgt ctcttgcaca tttccagatt ggatatcttt
    12541 cactactagt tgccattttt cttttgagac tgtacggtct ttcttatcag cagattgtat
    12601 agcttctcgt tctttacgca atacttttaa aaaaccattt ctgactatcc gaggaatgct
    12661 gtcgaagaac ccttctgtaa aaccatagcg acaatattgc ttccaaataa atagatagat
    12721 ttttggttgg atgaatcgtt ggtttttcgc aaaatattcc atctgagcag tgacttctgg
    12781 tgttcgttga cgttttgtct ttctcaacac ggaaataaat gtttcaaaat aagcattagg
    12841 atgtactggc acttcttcat atacgatgga ttgccaatct ttacgtaaaa atgcgcgata
    12901 gttgatcaag aattcttcta catcctttac taattcttct gcaaagtcgt caaatagttc
    12961 tttttttatc tgtcgctttt ttgactgccc attgatgtgt tcacttattt tcttgatttc
    13021 attttccaat gcttgataat ccgctatgta aatcggcatt tctacttggc gaaattctgc
    13081 gagccgctga gctaatttta tatccggtaa atatggctgt ttcatccgac gattttggta
    13141 caagacaaaa cagtattttt gccatagtcc ttcaggaaaa tcgatattct tctgcttaag
    13201 ccataataat tcgtctaagg ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc
    13261 cactgtcgga gagacatgca ggtgatcggc aaagtggttg atgcctagtt ttaggatctt
    13321 tccggttttt ggcgatttaa cgatgtattg cacttttaat tctctaccac actcacaata
    13381 caatttttgc tggcttcgtt ttgttcgaaa aaacggattg atcttgaagt cagaaaacac
    13441 ccaatctgtt gcagctaaat aatgttcttc cataaaacga gagctgatta gatatcggcg
    13501 gtgcttatcg actagttccc gctgtttttt cgttaatttt gcatagatct cccaacgttg
    13561 actgcttgtg tattgtttag aaattttgat gggatagctt ttgctgattt cttttggaaa
    13621 ccattcttca aagacggatt gccattcttc cacggctgtt tctttccttt ctaattacaa
    13681 aaagctggct actaacataa cgttagcaac cagccagtgt ttcttgctaa attagtgagt
    13741 tgttccggca tttccggaaa tatcttcttt attttctact gctgcttcaa ctgcataagt
    13801 caaagaagaa gcaatgtctt ctaaagacat agaaggcgta cccactggac gatcgatcac
    13861 ttgatcgggt gagaatggca cgtggatgaa cccacctcga attgccggga attgtgtttc
    13921 gatcaaatga agcaaacgat acatgatatc attgcagaca aatgttcctg ctgtataaga
    13981 gatgtacgct ggtagaccat gatcttgtac attttttgtc attgctttga ttggcaatga
    14041 ggtaaagtaa gcggctggac cgttttcttc taaagccgta tcgaatggct gatttccttc
    14101 attatcaggg attcttgctt ctgctagatt gattgcgaca cgttcgaacg agactgctga
    14161 ccgcccacct gcttgtccga cacagatcac gacatctggt tggtgctgtt ggatggcttc
    14221 tttgactact tgactgcttt tttcgaatac ggtcggtact tccactttga tgatttcagc
    14281 accgctgatt tcatctggca ttttttttac tgcttcatag gctggattca ctttgtcccc
    14341 accaaatgga tcaaaacctg ttactagtac tttcatctgt tgttcctccg tccgattgat
    14401 ttaacatatg gttatctttt atcataccat tttccttgaa cagcgtaaat aaaggaaaca
    14461 atagtttaaa taacataaca gatcaaactt aatatattct tataaaaaaa tagtgttcca
    14521 ctacaatgtt agaagaggtt gcgacaatta ttctgtcaca acccctctct tctattattt
    14581 aaatagaatc atataagcaa tttggaaaac tagcataaat aacgcaataa acaactggtt
    14641 tttgatcact ccgtatttgt ctttcatctc cagcatcgct acaggcacga tattgaagtt
    14701 agcagccatc ggtgtcagca gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat
    14761 taacgctgga ttcgctccat gactaaatac gaatggtgca ccgatcccaa ccgtcatcac
    14821 ggtgatggcc gcaaatgcat tccccatgat taccgtaaat atcaccatgc caagagcgta
    14881 aatgatgatg ccgatgttca catttccttc tggaacgatc gtacctactg cattggaaat
    14941 cactgtccca actccagcgc ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa
    15001 catgcttaac ggccctactg tgcccagcat atctgctgca tcatctaaaa atgtacttgg
    15061 tcgattatct cgagaaaaga acatcaagat tagcatggct gcaaaaacac cgacgcccac
    15121 gccgactaaa gcacctaaat tggtaaatag agcaaacaag atcgcaaaga caccgatact
    15181 caaagctgga ataaaaattt tcattcctag tttgtctgac attttttcca tatattcttt
    15241 tgatggaagc cggctttctc ctttgctgac tttcttagca attgctggta tggtcatcgc
    15301 aaagatcaag ccaccgttca cgattgccgg aagccatctt cctcctgcaa taactacccc
    15361 taagaccgtc cagaaaaagg ctgacccata acgatgagga ttggttttat ctgttaaatt
    15421 tcttactcca gtgtaaatca atacgagtcc catcacgata taaaggatct ccagtaattt
    15481 atcgcctaat aaaatatcag gatttgtaaa aaagtccatc ttattgcccc tttcctggat
    15541 aatatttctt cgctaatttc ttttctttaa gatagaagta gacacttgcc acaactaaag
    15601 ccgtgatagc cactggaact tccactaaag caagctgtcc caatgttacg tcatatccta
    15661 gatctttcag tgttgattgg actagcaacg cccctgctcc tccgatgaat agtacttggc
    15721 caaagaacca gcagacattt tccatagctg aagccatacc ttttagttct tcttcatgct
    15781 taggatggac ttctaaccct tttgattcga tggtccccaa tgccatcggt aaaatgattg
    15841 gacgaacaaa tccagcaact ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa
    15901 agatcccata aatatctaca atcataccag cagatacttt cttgaatcgc ttgatcaagg
    15961 aagccgctga ttctttcaga ccatttcttt caagcgttcc tgttgccaac ataatgatga
    16021 taaaaatcgc catcccgcga ttgtttacaa aacttgtacc taaaagttct aacattcctt
    16081 gaatgccaag accgccagtc aaagctgtaa caactaaagc aaccattacg atcaatatcg
    16141 agtctaattt aaatgcaaac ccgagaataa caatcaagac gcctaaaagg ctaagataat
    16201 tttccatata ttttcctcca taaaatgaca atattt
//
LOCUS       DACESX010000056        15924 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.056, whole genome shotgun sequence.
ACCESSION   DACESX010000056 DACESX010000000
VERSION     DACESX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.056"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            92..1519
                     /locus_tag="IXF88_001519"
     CDS             92..1519
                     /locus_tag="IXF88_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1508257.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1611..1976)
                     /locus_tag="IXF88_001520"
     CDS             complement(1611..1976)
                     /locus_tag="IXF88_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1508258.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1973..2338)
                     /locus_tag="IXF88_001521"
     CDS             complement(1973..2338)
                     /locus_tag="IXF88_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1508259.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2530..2778)
                     /locus_tag="IXF88_001522"
     CDS             complement(2530..2778)
                     /locus_tag="IXF88_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508260.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2777..2905
                     /locus_tag="IXF88_001523"
     CDS             2777..2905
                     /locus_tag="IXF88_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ1508261.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2962..4656
                     /locus_tag="IXF88_001524"
     CDS             2962..4656
                     /locus_tag="IXF88_001524"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ1508262.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4660..4779
                     /locus_tag="IXF88_001525"
                     /pseudo
     CDS             4660..4779
                     /locus_tag="IXF88_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5425..6558
                     /locus_tag="IXF88_001526"
     CDS             5425..6558
                     /locus_tag="IXF88_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ1508263.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6589..6909
                     /locus_tag="IXF88_001527"
     CDS             6589..6909
                     /locus_tag="IXF88_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508264.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6938..7402)
                     /locus_tag="IXF88_001528"
     CDS             complement(6938..7402)
                     /locus_tag="IXF88_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1508265.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7533..8786)
                     /locus_tag="IXF88_001529"
     CDS             complement(7533..8786)
                     /locus_tag="IXF88_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1508266.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8815..9132)
                     /locus_tag="IXF88_001530"
     CDS             complement(8815..9132)
                     /locus_tag="IXF88_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1508267.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9158..9478)
                     /locus_tag="IXF88_001531"
     CDS             complement(9158..9478)
                     /locus_tag="IXF88_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1508268.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9942..11357)
                     /locus_tag="IXF88_001532"
     CDS             complement(9942..11357)
                     /locus_tag="IXF88_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1508269.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11374..12690)
                     /locus_tag="IXF88_001533"
     CDS             complement(11374..12690)
                     /locus_tag="IXF88_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1508270.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12840..13538)
                     /locus_tag="IXF88_001534"
     CDS             complement(12840..13538)
                     /locus_tag="IXF88_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ1508271.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13827..14815)
                     /locus_tag="IXF88_001535"
                     /pseudo
     CDS             complement(13827..14815)
                     /locus_tag="IXF88_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            complement(14895..15728)
                     /gene="msrA"
                     /locus_tag="IXF88_001536"
     CDS             complement(14895..15728)
                     /gene="msrA"
                     /locus_tag="IXF88_001536"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1508272.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4954 a         3226 c         2722 g         5022 t
ORIGIN      
        1 ctttttcaga aattaactga tgatttcaaa aagaaaacat gtcataatat ttcccatatt
       61 gtgcaaaaaa atacaaggcg gaggctctac tatgattgaa gaatatattg agaaaaatat
      121 tttaagacag ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa
      181 gctctccaat ctcctgcatg tttgcaaaac cactttactc aatgatatca ataacataaa
      241 aaaagaattt gaagaacaga tcgcttatac acatcgggaa aaggaccgtt acactttata
      301 tttttcagaa catatacctc gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt
      361 tttaaaaact tgtctgcttt atttggaaga agatgaacct gattatcttc aattaactga
      421 atgtgaattc atctctgtca gcaaagctta ttcattaaaa aaacaagtat tagcttattt
      481 taatgactgc gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg
      541 tctgctgctt cttaatgtgt cgtatcgttt aggcggtttt aattcttggg agctacctga
      601 atcgtttttt gaacgtgccg atcgtttcat tgaatccgta acggaaaata gtggcagatt
      661 ttatgataaa gaaaataaag aaatcttatc gattggtttt gctatcagtt tcctacgcca
      721 gcaagtgtgt gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat
      781 ttacaattat gtcgaatcag catgggaaaa cactgatttc caaacctatt ataaaaaaga
      841 agaattcgcc tttattttga cactattcaa tctttgtaat tatggtttcc attcttatca
      901 gttgattgcg gaagactttc aacagctaca ccaagtgttt attgacaata ctcccgaaat
      961 aaaagaacta gttgctacct ttgaatctca cttcaatcaa gaattattcg gaaaccaacc
     1021 gttcgaacgt gcgctgattc atctgatgcg ttcggcttgg gataactatc aattattcat
     1081 gccagaaaaa ttttatcttt taaatgagga acagacaaat ttatataaag aagtccagac
     1141 aattttttcc agctggtcca gccagttacc ttatgatcta cggttgaatc ctaattgcat
     1201 gcgggctttt gtgatcgaat tgagcggaat tctccgtctg acaaaagaac atctgactat
     1261 ctatatcgtc acaaattcgg atgttcatta tctgatttat cgggaagcct tggaagctgt
     1321 aactactttc gattttcaag ttgcaccaac aatttattca agtatatctg atataaagaa
     1381 gtatgcgcag cagtcctcaa accgggtatt atgtgaacgt acattatata caccagatgc
     1441 ggtccaatat gaaaatatta ttcctatttc gatcaatacg atcgatcgag ctatcatttc
     1501 agctgtgcaa aacaaataag gggctgtgac aaaaatcatg tcacagcctc ttatttgttg
     1561 aagatgccca tttctgtaac aatctcttga tacacgtcag gctcacaacc tcacttattt
     1621 tggacatcta cgggtccgat ctctttattt aaaaaataag aaatgatggt tcggataact
     1681 accaacgccg ctagttggaa aatatcctgc aaggtaggtt tgataatcga ttcgatgatc
     1741 ccagctgaaa caagaatttc caaacttaga agtatatagc ctcccaacat ctttttaata
     1801 gcattgttct cgctgactcg gtcgctttgc acttttgtat ggacaaataa atatttcaaa
     1861 aaatccttga ttgatagaca aacaccccaa accaataaac agatagcaaa gatatcaagt
     1921 cccagtacaa gtttttcgaa atatggaaga agaaactgca tcaactctgt cgtcatccta
     1981 cctctcctct tctttgtttt tctttgtctt ctatttcctg attcaagaaa taagaaatga
     2041 ctgttcgaat gataataata agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa
     2101 ctgactcgat gatatcagca acgactagta cttccaagct taacaaaata taacttgcta
     2161 gcagttttct tgtatctgca ttacgagtat tacgttctgc atagtttttc ttgacatgag
     2221 accaatcaaa aagtttgaat agaacaagaa cgatcccgac aattaagata ccgatagaac
     2281 aaatatttaa aatagcaatg atccattcta gaaattcaat taaatttgag cgaaacatat
     2341 acgctaccta ctttctttta atttgttcct attcttttat tgtaaaagaa agttaccttg
     2401 ttcgtctatt taaaagttgt tctaatgata acggttcgct taaatttttc tttttcactc
     2461 tcccattttt ctccctccat ccataaatcg tggaagtatc caaaacaacg tagcaagtta
     2521 aaataaggct tagtccgtca tcagcttttc aaagaagcac tgtctcggtg ctttttttgt
     2581 acccaaattt ctaaaaatag aaccatacac ctcctcaaat cttttcttct tacctaccct
     2641 atttttattt acaggaaaat cagacacttc ttcttttttg tcaaaatttc ctttttcttc
     2701 tcgtcctatc atttggcatt ctaataaaaa cgaactatcc ttggatatag aaagagaaaa
     2761 caaggaggcg tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct
     2821 tgcccttggc gctgcatatc taggaaaaaa gacaggacta ttcgaagatg acagtcattt
     2881 atatgatgaa ttcgaatcaa tctagcatcc tatttagttg aatcactacg gacatacttg
     2941 gaagacattg gaggaatcac aatgagaaaa agtaaagcaa taatgattgg tgccggtatt
     3001 gccaatatgg cagcggctgt ttatttgatt caagaaggca aatggaaagg ggatcagatc
     3061 accttttatt cattggatga tcacggttca aatgatggtg caccgaccga cactgtagtg
     3121 gatgaatatt ggaataaaaa ccatccatta gaaaaccaaa aaggctatat tgctcgaggc
     3181 ggtcgcatgc ttaattaccg tacctatgta gatttgatgg atctgttaag tcgaatacct
     3241 tctgttactg agccagggat gactgctgaa gaagatacga gagattttga tgcaaaacac
     3301 cgaacctttg acaaagcacg tttgatggaa ggtggcaaag gaatcattga tggcggaaaa
     3361 ctgggattca ataataaaga ccgtattctt ctgactaaac tgattgctat gcctgattca
     3421 gaagaagaaa aactagacaa tatcactatc gaagaatatt tcaaagatga tccacacttt
     3481 tttgaaacaa acttctggtt tatgtgggaa acaacatttg ctttcagaac acgtagttct
     3541 gctcaagaat tgcgtcgcta tatgcatcaa atgatttatg aattcactca aatcgaacat
     3601 ctagtgggcg tcaatcgaac acgttacaat caatacgaaa gtattatgct tcctttgatc
     3661 gagtacttga aagaacaagg atgcaaaatc attttgaacc gccgtgtcac tgactgggaa
     3721 ttcaaagaaa caccgatgca agatgaaatc actgtgactg gtttgaaaat taccaataca
     3781 ctgactcaag aagaagaata cgttgtggtc gatgaagata cagcagttat tttcactaat
     3841 gggtccatta cagattcagc tactttagga gattacgaaa caccagctgt agaaaatatg
     3901 gattatggtg cagcttctag tctctggaaa aaagccagtg aacgtttcta caacttggga
     3961 aatcctgaca aattctttgc tgatcgagat gcaagcgaat gggtcagctt taccctgacg
     4021 acgaaaaacc atttgttgtt gaatgaaatc acccgtatca cgactcaaga accaggaaat
     4081 gccttgaatt catttatttc aaccacaccg atcacgccgt taggccaaaa agatgtcaat
     4141 atgtctatcg tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt
     4201 atttggggtt atttccttta tccaagacga cgtggcgaat ttgtggataa acagtatatt
     4261 aaaatgaatg gaaaagaaat gctagaagaa ctgattggac aactttcaaa agtagatcct
     4321 ggaccagtga atattcgtga aaaagaaaca gaaattttcg acagcgtaat aaataatatc
     4381 cctgtctata tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca
     4441 aaagtcattc ctaaacattc gaccaatctg gcctttacag gagaatttgt cgagcagcct
     4501 tatcagatga tctttactga acaaagtgct gtacgttctg gagaaatcgc tgctttccat
     4561 tttgcgggta ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa agatattcct
     4621 actctagctc gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc
     4681 ctgcatcaag taataagaac aaccaggcta aaacagcttc ttatgttttt gatttggttg
     4741 gacttattac cgcagatgca agcttttgaa caccgtttac caagaggttg tgaaaaagct
     4801 gccctgcatc aagtaataag aacacccaag ctaaaactgc ttcttatgtt tttgacttgg
     4861 ttggacttat taccgcagat gcaagctttt gaacaccatt taccaagagg ttatgaatgc
     4921 cctgcatcaa gtaataagag cagccaagct aaaacagctt cttatgtttt tgacttggtt
     4981 agacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagg
     5041 cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa
     5101 atttgactta ataccgagaa actgatccct tctgtcataa cctcttttta tttatgtctg
     5161 tttctttttg aaaacaacta ttttttcaac gtttactctc ttgccaatcc aacatcatct
     5221 aacggataca actatttcaa tgattaccat tacactaaaa aatcggaaac acacctctcc
     5281 gaacacaaat acataagact atagaatgtt tcatttaaga aaaaaagcgt ttcattatca
     5341 aaaaggtaag atctccttta aaatgaaatt atctgtagta ctaataatgc agacaatttt
     5401 atgaatctga aaggagcatc tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg
     5461 gaaatcagaa acgtagatga tccaaaaata ttagaaccaa cagatgcaat catccgtatc
     5521 acagcaacag ccatttgcgg ctccgatctg catctttacc atcatggtga agcagttttg
     5581 gaaaaagggt atgttgtagg acacgaacca atgggaatcg ttgaagaagt gggatctcaa
     5641 gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt ttaatattag ttgtggtcat
     5701 tgtcatttct gcctgaatca catggaaagc caatgtgatg aatcaaatcc agatcagtta
     5761 tatggcgggc ttttcggatt tggcaagttg aacggtaatc attggggtgg tcaagcggaa
     5821 tatctaagag ttccttatgc agactctact tcatttgttg taccagataa tgatcttcct
     5881 gatgaaaaag ttttatttct atcagatatt ttgcccacag cttattggag cgttgaaaac
     5941 gcaggagtaa aaaaaggcga tacagttgtg attctaggtt ctggtccagt cggtctattc
     6001 gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa tcgcagtaga tccagtacag
     6061 caccgtctag ataaagcggc taattacaat ggggttgaaa cttatttctt agaagacacg
     6121 gcacaagctg gaacagaatt gtatgaactg gctaaaggtg gcgcagatgt cattatcgat
     6181 tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag cgaaaaattt agtaagctta
     6241 caaagcggca caatctctcc attacagatg gcatcgcaag ctgtcaaaaa atttggaaca
     6301 gtccaagtaa ctggtgttta tatgactcca gcttcttctt acccgctgca agaatttttt
     6361 atgcgggata tcgctgtcaa gcatggacag gcaccaatta tccatctaat gccaaaaatt
     6421 tacgcaatga ttgcagaagg actattcgat cctacacaaa tcattactca ttcgctgcca
     6481 ctatcagaag cagcacaagc atatgagatc tttgacaaaa aagaagataa aaatataaaa
     6541 gttgttttga aaccttgaat tcagtaaaaa atacaggtga ttttctttat gcccaaaatc
     6601 aaacgaaata cccatgagat cgaaatagga aaagaagaag atatcgaaat acgaggacgt
     6661 cgcttccgct tctatttcca aaatcgttac acattgatct cactggctgt cgatttatta
     6721 accgggattt tctatatttt tggaagcctt gcttccttaa ctaaaatccc cgatcgatat
     6781 ggcacctatt tttatttggc tggtgctatt ttcttgacga ttcgtcctat tctcagaatc
     6841 gtaaggaaca tttttattta cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa
     6901 gaggcatgag aatttttcaa atctcatgcc tcttttttca ccctatcgta tcactttgat
     6961 acagacgagc ggtcgtccca ctttcttcct tgaccattgt caaaaatgtc cactcgtatc
     7021 gttcataaaa cgtggtatga tctgtaatta agtaaagttt ttcgtaccca agttgatgca
     7081 tttcttttct gatttccgct aatatccgct tggctatccc ctgcttacgc ttttccggtt
     7141 caacaaacag ggcacaaaga tttggtgtca gatcagggcg gttatggaag tcgttttcaa
     7201 tgacgcccgc tcctccgacg atggtttctt tttcatctaa gaccacatac cattgaggaa
     7261 tactttcctt attagcgatt ccagtacgaa tactttcctc atacacaaca gcaggaactc
     7321 cccattttga tgaaaaccaa ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt
     7381 tgatccagtt atatgtaacc aatgtttaaa cctccgacta aaataaaacg tttacaaaaa
     7441 ttatacaagt ctttatgaaa attagcaatg acaacaatag tgatgtaaaa aaaaaacgga
     7501 aaacaatcac ttgttctccg ttttttgatt gatcaagcat ctaaagcttc ttttcttctt
     7561 tcttggatcg tagctacctt gacgaacggt agatagatca aaatagaaag aaccaaattg
     7621 acagcggcta gtacaccacc tgcaatactt tttgttgcca atacaccgcc aataattggt
     7681 ggagttaccc atggaggcat aacggtagct gctggcacta aacctgtaga agtcgcaaag
     7741 taagcaacac taactaagac cattggtgtc aaaataaatg gaatgaacat gatcgggttc
     7801 aacacgatag gtaacccaaa catcgttggt tcattaatat tgaagacacc tggcgcaatc
     7861 gacaagtttg ttacaaccat atatggtttg tttttacgtc ctactaaata aatagctaac
     7921 agtaaaccta aagttgcacc tgttcctcct aagttgacaa agctgtcaaa gaaaggtttg
     7981 ttcacgataa acggtgcgct atgcccagct gcgattgcat taacgtttgc ttcaatggct
     8041 ggcgcattga ttgtttgcat caaaggatcg accatattcg ctccgtgtaa accaaagaac
     8101 cataagaatg gtgtgataaa tgcaagcagt aaggcagatg gataagagtt tgcaagtccc
     8161 ataaatggtt cttggaccac tgtatagaaa gaacctacga tatcatcaac accgaatgca
     8221 gcaaaaattg cagcaactaa agcaaacgca gagatagtga tcattgctgg tagtaatgaa
     8281 gcaaatgctt ttgcaactgc cggtggcaca ccatcaggca ttttgatgat caatttttca
     8341 tttccgctta aacgtacaaa gatttctgtt gaaataatcc caacaataat agcaataaac
     8401 aaaccgtttg agctcatacc taatgctccg cctaaagcga agaaagagcc taaagaaatc
     8461 acacctgcgg aaacaccgtc ttttcggtag ccgttagcta aatgataagc aagcaagaaa
     8521 gcaatcaaga cagaaagaat ggcaaatgta ccgttccaag cagcagcccc gaatcctttc
     8581 catgattcac caccaaaaat tgagttcatc aattcttgga acccgggaat cggtaagtta
     8641 ttgatcatta ctgctaatgc tcctaagatc attaacggca tactcaccat aaatgagtcc
     8701 cgaatcgcaa ctaaatgccg ttgtgcacca atttttgatg ctacaggtat aaaatgtttc
     8761 tccataaatg caacaaattt atccataaat cttccctctt ttctttactg tagattacgc
     8821 gttcaatcga cgatacagat caatgatctc ttttgctaaa tctgtaaaag ctatagatgt
     8881 catcagatga tcttgactgt ggaccgtcaa aagcgtgact tcaacgtgat ttccttgtgc
     8941 ttcttgtgtc agcatctctg tttgagagtg atgtgcttgg actaaagatt cctctgcatc
     9001 tttgattttt tcatctgcta aagtgaaatc accttttttc gcagcagcaa tcgcttccat
     9061 tgcatcgctt tttgcatttc cgccaaacat gatcagaccc atgatggctt ctagattttt
     9121 ttgatcttcc atttattgta tcctccgata atttttatta tttgtttatt agtttttctg
     9181 cttggtcgag tactttttca ccattcatca tgccatagtc agccatattg attacatcta
     9241 aaggaatgcc tttaggtgct agcttttcag caaattgatt ttgcataaag cgtacttgcg
     9301 gtcctaaaag taaaacgtct acatttttag attctagttg attatccgca tctgaagcag
     9361 atacagcaaa gatgtctgct tccattcctt ttgcttctgc tgccttttgc atttttgtga
     9421 ctaggagact tgtactcatt ccagctgaac atacaagcat gattgttttc ttttccattt
     9481 taaacacgtt cctttctata cttacacttg tatataagca agtagcgtgc caactttaaa
     9541 agcgctttaa aaagttgata tcatcacatt taaggtctat accaagttgc atacacacta
     9601 aaaaagaaag cgcacactaa acacacaaat taatttttat tacctttttt cttttaacgt
     9661 gcacaaaaaa agttgtgaca caaaacttgt cacaactttt ttcaaataaa cggtattcaa
     9721 aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa cataagatgt tgtttttact
     9781 ttgctattct tattacttga tgcaggacag ctttttcaca acctctcaat aaacggtgtt
     9841 caatcagctg accctgggta ttagctcaaa acgctggatt cacaacctct tttatttcaa
     9901 ataaccagta aatacaatga acatctacaa aaaacttcta tttatttata gatttcgtca
     9961 ggcaaatcca attctctctt atcaatcaaa gtctgatacc acgaagcaga ttttttcaat
    10021 gttctgcttc gttcatcgtt caattcgatt cgaactaatc catatcgatt cttaaatgca
    10081 ttcattggtg acacacagtc agtgaatgcc cataacatat agccttcgca atttgcgcct
    10141 tcttctgttg ctttgatcaa gtaatgcaaa tgctgtccga taaattcgat acggtaatca
    10201 tcttgcacga ttcctttctc atccatatac tgttcttcat attctcttcc cataccgttt
    10261 tctgttatca gccaagggat attatgataa ttttctttta gatacatggc tatatcatac
    10321 atgattttcg gatagatttc ccatcccctg gaattattca ttttcttccc aggtaaatca
    10381 aagacttcat aatatcgttc aggatggaaa ggtgtctctt cattccattg ataacgaggg
    10441 gcgcgaacgc gtttaggata atattgattg attccaagga aatctactgt atgatgctga
    10501 atcgtttcta aatcctctgt tgcaggatcg aaataaatcg attgtttttg acacaactcc
    10561 aggaactctt ctgtgtagta tccttttacc atcggatcaa aaaatactcg attataaaac
    10621 aaatcataca tataagcagc tttttggtct tctggagaat tggatcgagc atagaccatt
    10681 tcaggattta aaatacagcc aatccttcca ccataatttc cgctgcggta aatctctact
    10741 gctttagccg ttgctaaaac tttatgataa ttccacatca tccacttttt tgtgttttgt
    10801 tcatgcggcc agcgaacagc atccaaatag caacgcgttt ggggaacgat tggttcgttg
    10861 aatgtaaacc agttttttac cctatcaccg taacgatcaa aggcaatttg agcatactta
    10921 gcataaaaat caactacttt tcttgaagac cagccatcat atttttccaa aagaaaagct
    10981 gggacttcat aatgttccaa acaaatcatc ggttctacgt ctgcttcgat caaagagtcg
    11041 atcatttcac ttatatgctg agcatattct tcatctacta tacagttctc ataatcagta
    11101 aaaaaacgag cccaattgat agaagtccga taatgtgtca gatggatgga tttcatcaga
    11161 tccacatcct gctggtattg ttccataaaa ttagttgcta cggctgggcc atacccatta
    11221 tgccaaacaa aatgttcatt tttataccag ctatctataa acgaatcctg tccttctttt
    11281 tttcctttcc aaccttccgt ttgccaagca gaagatgcag caccaagaat aaaatttttt
    11341 ggaatagtaa ttttcatgat ttcctcctgt tttttagatc ccatcatttt ccacacgatt
    11401 tgctactttt acaaatggca gatagatcaa aatagaaaca ataatacaaa caagttgcgt
    11461 aataactgca ccgatatttc ctgctgttgc cagataagca ttgacgatcg gcggcattgg
    11521 ccaagggacc attacaactg ctttcgccgc aaatcctaaa gcggttgcaa aataaccaat
    11581 cgttccagtg ataagaggcg ttaagataaa cggaatagct aaaattggat tcagcatgat
    11641 cggcatacca aaaataactg gttcattgat gttgaagatc ccaggagcaa gggataactt
    11701 ggtgatttgt ttcatatctt cccgttttcc aactaacaat actgcaatca gtaaaccgat
    11761 cgtgactcca gacccgccca tactcatata catatcccaa aatggcatag taataatgtt
    11821 cggtacttct tttccagctt caaaagcttc cgtatttaca gcaattgccg ctaaaagcaa
    11881 cggttcccgt atcggcttca ccatttgatt tccatggata ccaatcaccc aaaagacttg
    11941 cgcaataaac atcaatagca aaatacccgg taacccttgt acgacacctt ctaatgggcg
    12001 ttgtacgaca ttgtaaatga tatcataaag gtacattcct gtaagttctt tgatgataaa
    12061 tcccgcagcc gcgatacccg atacagtcag aatcgtcggg aataaagcag aaaaactagc
    12121 agaaacattc gttggcacgg tatcaggcat tctcagctgt agtttcttct gtttcctcaa
    12181 ccagcaataa agttcaatag aaaggatgga gaccaacatt cccaaaaata accctttcgt
    12241 atctgtatat tgtttagcca atacgttttc tactataaca gtctgatttt cattcactaa
    12301 ttcataaaca gttggattaa cagataaata agacatcact gcgatgatac cagggaatac
    12361 accatccact ttattcagct tggctaattc gatccctatc agaaaaacag catagatggt
    12421 caagaaactt agtgtgacat agctgatggc agatgcgata ggctttaatt cttctaaaaa
    12481 acgcaagaaa cttatctgtg ctaagccatt ttccgagtca aagaccattg ctgaaaacaa
    12541 tgtgccaaaa gcacccgtaa taattaccgg aatcaatgca gcaaatgcgt tttttatcac
    12601 catgatatac cttaaattat tgattctcgt tgccacgttc cctaaaacat tggctaattt
    12661 atccataaaa ctcattccag atcccctcat tcctgcttct tctccttgac gtcatctctt
    12721 tttgtctatt agtcagcgct tacatttcga tgaacagaaa ctatttactc ctctttttta
    12781 ccaaatataa aacaaaaatt catctgatgt atttatcata aaatacatca gatgaactgt
    12841 caattagttt ctttttctcc tattattttc tgtaattctc tccgatttat tgccatatgg
    12901 acagtcattg catcaaacgc agcaagtgca ttccgccttt cgatggcctg aacgatctct
    12961 gtatgaatat cgatcgtctc gattttcaga tgctggatat cataattttt attaaacaaa
    13021 gcaattgatt cattaatgat tgggataata tgcagcaagg ctacgtttcc gctagcttct
    13081 gcaatcatcg tatgaaaatc gatgtctaat tgaacatgtt cttgtgtgcc ttctcgaaaa
    13141 gaagtttcaa tcttttgcgt taattttttc atttggacga tttgattctt cgttgcatat
    13201 tccgctgcca aacctgccat cttaggctcc aataaatatc tcagttcaaa taagtctcgt
    13261 atcatccgat cctgatcttc aatcaaagaa aaaccgagtg ggtcattggc aatccctcgc
    13321 ttatcactga caaatgtccc cgctccttgc cgaacttcaa ggatattcct ggaagctaga
    13381 gcccgcacag cttctcgcac cgtgcttcgc cccacctcta aataatcgga caattcatat
    13441 tcattcggca gtttatctcc tacttgcaac tgcatttcct ttaaatatct aaggatcttg
    13501 tcagtagtta cttctactag ggttttattt tcttccaaac cacatacctc cttttcatct
    13561 gatgtatttt ttcgtaatta ttataacacg tttgaatcat ctgatgattt tttgtataca
    13621 aaattgatta atccgttcat agacatacaa acgaagaaga tatttaccat aaaaaagact
    13681 aggataaaat tcactcctag ccttcgatca tttttatttt gaacgacaaa aaatatgatg
    13741 ttcaataaca gacgatcttc attcgattct tgatgtcatc ttttgttttt tttaatatat
    13801 ggataaaaag tttgattaca tcgtttctat tctagaattt ggtcgattct caattcttct
    13861 tctggtgaga aatctaaatt atccatcatt cgaacacttt cttgtaactg acttaaccga
    13921 cttgctccga ccagtacact tgtaacagat ttttgtcgca aattccatgc aagggccatt
    13981 tgcgctaatg attgtcctcg tgatacggcg attgtttgca gtgctttgat tttttccaga
    14041 gttgatccaa cttgttcttc tgaaaggaat ggtatttcct tacgatgtgc tctagaatct
    14101 tcaggaatac catgtagata acgatttgtc agcaaccctt gagcaagcgg actgaacgtg
    14161 attgccccta gcccttcttc ttctaatact ggagatagat cgtcttctag tagtgcacga
    14221 gaaaacatat tgtagcgcat ctgatgaata atgaacggtg cttcctttct ttttaaaatt
    14281 tcagtcattt ttttgtatct tctccattat agttggatat accaatatat aatgcttttc
    14341 cttgtcttac aagttgcatc aaagcttctg ctgtttcttc caaaggtgta tcagggtctg
    14401 ggcgatgatg atagaagatg tcaacataat ctagccccat tctttggaga ctttggtcac
    14461 aacttgaaat gatggattta cgagagcccc attccccata tggcccttcc cacatatgat
    14521 aacctgcttt gctagaaata atcagttcat cacggtaggc ttgaaaatct tctctaaaaa
    14581 tacgcccaaa gtttttctct gcagctccag cagggcctcc ataattattc gcaaggtcga
    14641 aatgggtaat tcccatgtcg aatgcgcctc ttaaaatctc tctttgatta tggataggat
    14701 cataatcacc aaaattttgc cataatccga gagataaaat cggtaatttc aaacctgaat
    14761 gaccggattt acgatattcc attttttcat aacgtgcact atctgcaata tacaattttt
    14821 ctcctcctca tcaaatcttt ttaaaacgct ttcttttttc tatttttagt ttactacatt
    14881 ttaaagaaaa ggagttaatc aaaatgattt tttcgtatat ttttttgcca tttgtttttt
    14941 actcgttgat agatcagaaa ttgattacgt gcttgctgga tttttttata acgtttcggt
    15001 tgttttttgt aaaattgctg atgatagttt tctgctggcc aaaacgtacc ggctggttcg
    15061 atttttgtga cgattggttg gtggaaagtt cctgaactgt ctagtttctg ttttgattgt
    15121 tccgctaatt ctttttgtcg ttcgccttga taaaaaatca ctggacgata ttgcttccct
    15181 ctatcttgaa attgtccaaa tgtatctgtg ggatcagtaa tttgccaata gattgtcagc
    15241 aactcgctat aacttatttc tcttgtgtca taaatgatct ccacagcttc cacatgacct
    15301 gtgtaaccac cacttacttg atcataattt ggtttttcaa catgtccgcc cgtgtatcct
    15361 gacaaaacag ataggatccc ttttttcgat tcaaacggct cgaccataca ccagaaacat
    15421 ccccctgcaa atactgcacg ttcttgatag tcagcatctt gcatggcatg cttggaaaag
    15481 tcatattttt gctccttatc aaatccttcc gtaatcaata aataaaagtc cattacatca
    15541 ggcgtcagat tcattcggat agctaatgga cgtagtgttg cttctagttt tgataactgt
    15601 tctttaacgg atatattttc tcttatgtca ttctttgtct gaatcaatac ttttcgttcc
    15661 caatctcgtg tattcggatt caaaatcaga ttataaagtg tttgaatgaa tatttgttcc
    15721 tcatccatag attctcctcc tttccgaaga agacaagcta gtgactttag ctgtaattta
    15781 tcataaagtg aggaaataag cgattacttc atccccctac agatttttcg tataaaaaag
    15841 agagaatcct tatcgacaga ttctcctttt taatcaattt attttataaa tgaaaagtta
    15901 atttaccttt tatggaaaac tatt
//
LOCUS       DACESX010000057        15763 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.057, whole genome shotgun sequence.
ACCESSION   DACESX010000057 DACESX010000000
VERSION     DACESX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.057"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            39..791
                     /locus_tag="IXF88_001537"
     CDS             39..791
                     /locus_tag="IXF88_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1508273.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            788..1702
                     /gene="pfkB"
                     /locus_tag="IXF88_001538"
     CDS             788..1702
                     /gene="pfkB"
                     /locus_tag="IXF88_001538"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1508274.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1720..3651
                     /locus_tag="IXF88_001539"
     CDS             1720..3651
                     /locus_tag="IXF88_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1508275.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3762..5321)
                     /locus_tag="IXF88_001540"
     CDS             complement(3762..5321)
                     /locus_tag="IXF88_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1508276.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5476..7710
                     /gene="pcrA"
                     /locus_tag="IXF88_001541"
     CDS             5476..7710
                     /gene="pcrA"
                     /locus_tag="IXF88_001541"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1508277.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7727..9763
                     /gene="ligA"
                     /locus_tag="IXF88_001542"
     CDS             7727..9763
                     /gene="ligA"
                     /locus_tag="IXF88_001542"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1508278.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9869..10174
                     /gene="gatC"
                     /locus_tag="IXF88_001543"
     CDS             9869..10174
                     /gene="gatC"
                     /locus_tag="IXF88_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1508279.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10174..11646
                     /gene="gatA"
                     /locus_tag="IXF88_001544"
     CDS             10174..11646
                     /gene="gatA"
                     /locus_tag="IXF88_001544"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1508280.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11643..13073
                     /gene="gatB"
                     /locus_tag="IXF88_001545"
     CDS             11643..13073
                     /gene="gatB"
                     /locus_tag="IXF88_001545"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1508281.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13083..14138
                     /locus_tag="IXF88_001546"
     CDS             13083..14138
                     /locus_tag="IXF88_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1508282.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14249..15622
                     /gene="rlmD"
                     /locus_tag="IXF88_001547"
     CDS             14249..15622
                     /gene="rlmD"
                     /locus_tag="IXF88_001547"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1508283.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15625..15753
                     /locus_tag="IXF88_001548"
     CDS             15625..15753
                     /locus_tag="IXF88_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1508284.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5366 a         2894 c         3462 g         4041 t
ORIGIN      
        1 aaaaatatgc tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgtagac
       61 aaaaaatcct agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc
      121 tttttaatgc ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt
      181 ttctagaaag agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata
      241 tgagtgaaaa atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg
      301 aaaccattaa tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga
      361 ttccttttct gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa
      421 atttagttga tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta
      481 atgctgtttt aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat
      541 ttatgggaat gaacggtgcc catcttgaac aaggattcac gactccggac ccagaagagg
      601 cagctgttaa acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata
      661 ccaaattcaa ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa
      721 ccgagcgttg tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg
      781 caatacaatg atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga
      841 tgggttaaaa ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa
      901 agggatcaat gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt
      961 tttaggaggg ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc
     1021 tttgttcaca accgtaaaag aagatacaag aatcaatatc aaactaaaat cagatactga
     1081 aactgaaatt aacggtttgg gcccatctat ttcagatgaa gaaatcgatg agttgaagca
     1141 agcattagga acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccggctag
     1201 cctgcgaaaa ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt
     1261 cgtgattgat acaacaggtg atgacctaaa aaatgcactt gttaagaaac cattgttgat
     1321 caaaccaaac aatcacgaat tagctgagct ttacaataca accttccaat ctgtagaaga
     1381 aatcattccg tatggtaaaa aattgctagc tgaaggggca cagaatgtgc tgatctcaat
     1441 ggctggagac ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt
     1501 tcgcccattg aaaaattcag taggtgcagg ggattcaatg attgccggct ttattggggc
     1561 ttacagccgt acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc
     1621 aacagctttt tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt
     1681 tgaaatcaca aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg
     1741 gtcaaagaag cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa
     1801 atggtccaaa aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga
     1861 attctagccc gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac
     1921 gcgaaaaaca gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta
     1981 gattatgaag ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa
     2041 ggcgcaaacg acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca
     2101 gatttcgtag gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa
     2161 acagctgaag agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa
     2221 gcttctgctc atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc
     2281 gctcacactt acatggcaga agatgctctg aagaaaaaag cgaaagaaat gggtgtggag
     2341 atcaaagtcg aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc
     2401 gcccgtgcag aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac
     2461 ggaaaagaac tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagctgatc
     2521 aatctagccc ttagtggtac tgcaccagta ttccatggtg atggaaaaga aagcagttca
     2581 gaagaaggaa atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc
     2641 gtatcacaca tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg
     2701 atcgaccaat ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa
     2761 gctgctagct ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta
     2821 gcaggattta ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct
     2881 ggtgcattag ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta
     2941 gctggttatg tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat
     3001 ggaattaaaa cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg
     3061 ttgctggtca atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt
     3121 atgagtggtt cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat
     3181 ttaggcggtc caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact
     3241 gttgcaactg gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca
     3301 ttggcaattt tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat
     3361 gctggtttga cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt
     3421 gcagcagctg acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc
     3481 ggattagtcg gagctttcgg gatcaaatta ctggctcctc atggcgggat tttcgttgta
     3541 ttcttactga gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct
     3601 ggtgtgattt atggtttatt gagaaaagct cctgcagcag cagaagcata gaaataaaaa
     3661 agaagaggct ggtttttccc agtctcttct ttttttatag aatcatatag aatcaagtag
     3721 tgtagatgaa tagttgttgt aaaatagttt ttccatgttc gttaaaatga ttgcttcaaa
     3781 tttgtgatag aagatttagc ctcgatttcc tgcttgattc ttggcaagtt tgcttcatca
     3841 gtcaagataa ccaaaatatc tcctacattc atgatcgtat caccgtgagt caagatttcc
     3901 tgatttcctc tgcgtataga gattagcagc atatttttag gccaattgaa gtctcgtacc
     3961 attaatccgt ctaaagaact ctctgccgtt actggaaaat caaaagttgt ccttgcacct
     4021 tttagtgagg cggtctgagt atttccaagt agacgttcca ataatgattc gtaaattgga
     4081 ttaccgccga gaaggtcatt gacgatataa gcaacaagag cagccaatcc taaaggcatc
     4141 agatgattaa gagagccaac catttctgtc actaaaataa ttgctgtcag cggtgctttt
     4201 ccaatcgctg tgaaataacc agccatggca ataacgacaa aatccttgat gtagattgga
     4261 tccatcccga aatcaaccat aatgtttcca taaaagctac cgattaaagc ccccaatgtc
     4321 aatatcggaa ggaaaatccc ccctggaaga ccaccaccgt aagaaatcat ggaaaaaacg
     4381 aatcgaagaa caaacaaacc gataatcata atgattggca aggaagcatg tccaaaagct
     4441 aaaacgattt gattgcctcc gcctagagta ctaggccata aataaccaat tggcaaaatc
     4501 agcaagaaag gaacgaaccc gtgaaaataa gctggtaaag gcaatcggtt gtaaaatttt
     4561 ggcaaagcca gcaagacctt ctgataaata aaacctaaga tgcctagaag tactcctaag
     4621 atcactaagg aaccataata tttcaacggc aaagaaggaa ggtcacctag atataaaaca
     4681 ggtctcaagc cgaaaaagta aagagagacg aagttagcag tgattgctga agcaaatgat
     4741 gtcagccaaa caagtggaga aaaggagtga tggatttctt ctaaaacaaa gagtaatcct
     4801 gcgataggag cattgaatgc tgctgcaagt ccgctacttg caccgctaga aattaaaatc
     4861 ttttcttctg aagatggact ccgaaacaat tgtccagctc cttgtccaat ggatgcgcca
     4921 agctgtatag aaggtccttc tctacctaga aaaaggcccg caccaactga aagtacaccg
     4981 ccaatgaact ttttccataa aacagagaac cagttcattg ataattcccc gcgaagctgt
     5041 ccctctactt gtggaatccc gcttcccttg atatccggtt catttttgac cagataaccc
     5101 acgaaaaatg ctgcaaggat gcagcaaata gctaggccaa ttaagtaaat cggctgttcc
     5161 cgactcattt catagatact tcgaactaaa tcgcttagct tttcgataga taatcgaaac
     5221 aggctaacga cgattcctgt cagcagtcca acaaatattc cttttagaat aaaatatatc
     5281 tgagtcttat ctaatttctt gatttttcct tttttttcca ttcaaacgcc tcgctttctt
     5341 ttaatttagc gcgaaatcaa cgaattgaca aggaaaaatc cataaaaaga aacaaatgat
     5401 tttgaactag ctagtttaat gaaagccaag aaaatgctat aattaacaag attgataaaa
     5461 gaaaggatgt tgttaatgtc atccaaagct gaattattaa acgggatgaa cccccgtcag
     5521 aaagaagctg ttcttcacac tgacggacca ttactgctaa tggcaggtgc aggaagtgga
     5581 aagacgagag tgctgacaca ccgcattgcc tatttgattg aggaaaaaga agtcaatcct
     5641 tggaacatcc tagcgattac ttttaccaat aaggctgcaa aagaaatgaa agaacgtgtc
     5701 aatgcgattc tcgcatctgg aggcgaagat gtctgggtat cgacttttca ctcgatgtgt
     5761 gtgcgtattt taagaagaga tgttgatttt atcgggtata accggaactt tacaatcatc
     5821 gattcttctg aacagctgac cttgatgaaa cggatactaa aagaactgaa cattgatccg
     5881 aaaaaatatg atcctcgaag tatattagga acaatctctc aagcaaaaaa cagcctgcag
     5941 acccctcagg attttgcaaa aatgcaaggc agttattacg aagagattgc ggcaaaatgt
     6001 tatgctgctt accaaaaaga gcttcaatat aatcaatgta tggactttga cgacctgatc
     6061 atgaatacga ttcgattatt tgaagaacat cctgattcac tcacatatta tcaaaacaaa
     6121 ttccattata ttcatgtaga tgagtaccaa gatacaaatc acgcacagta tacgctagtc
     6181 aatttattgg caggtagatt taggaacctg tgcgtcgtgg gagatgcaga tcaaagtatt
     6241 tacggctggc gcggtgcaga tatgcagaac atcttggatt ttgaaaaaga ttatccagat
     6301 gctgcagtaa ttttgctaga acaaaactat cgctccacga aaaacatcct tagcgcagca
     6361 aaccaagtca ttgaaaacaa tagcaatcga aaaccaaaaa acttgtggac agaaaacaaa
     6421 gaaggaaaca agatcactta ctaccgtgca gataatgaac gagacgaaac ccgttttatc
     6481 gtagatcgga tgcaagaaga gattcgaagc aatcatcgga attacggcga ctttgccatc
     6541 ttgtaccgga cgaacgcaca atctcgtgta atggaagaaa ctctgttgaa agcaaatatt
     6601 ccttataaaa tggttggagg gcacaagttc tacgatcgta aagaaatcaa agatatttta
     6661 gcgtatttga atgtgctggc aaatcctcaa gattcaatca gttttgagcg tatcgttaat
     6721 tcgcccaaac gaggcatcgg accaggatct atcgaaaaac tacgcagttt tgcttctctt
     6781 catgaatggc cactgcttga agcagcgcaa aatgtggatc tggcaaatat cggtggaaaa
     6841 gcaggacagc aattaggtgc atttggagag atgatccaag aagtcactca gatgatccca
     6901 tacttgacag tgacagaatt gacgaaagag gttttggatc gtagcggata tctagaagac
     6961 ttaaaaatcc aaaatacatt agaagcacaa gcacgcatcg aaaacttgga ggaattttta
     7021 acggtcacac aagaattcga caaacaattc gaacagcaaa atgaagaaga cgctgatgca
     7081 ccagaagaaa aactaacggt cttcttaaat gatctggcac ttgtttccga tattgacaat
     7141 ttagaagaag atgcttccca agtgacattg atgactttgc atgcagccaa aggattagaa
     7201 ttcccagttg tattcctgat tggtctagaa gagggggttt ttcctttatc tcgagcattg
     7261 atggaagaaa gcgaactgga agaagaacgc cgtctcgcat atgtggggat cacgcgagca
     7321 gaagaagcgt tgtatttaac aaatgctttc tccagaacat tatatggacg gacgcaatat
     7381 aaccgacctt ctcgttttgt agaagaaatc gatcaggagt tactggaaat cgaaggcatg
     7441 cgtcctacgc ctaaaaaaac acctgtattc gctaaaaaaa cagcatatag ctataaacag
     7501 ccagagacag cggttgttcc ctctaaaagt gctactggag gggaaaataa cagctggaaa
     7561 cctggagata aagtcaaaca caaaaaatgg ggtctaggaa cagtcgtccg agtaagcggg
     7621 acgtcgaagg atctagaact agatgtggct ttcccaagtc aaggagtaaa acgtcttttg
     7681 gctgcctttg cgccaattga aaaagcttag tagtggaggg taaatgatgg aacatgaaat
     7741 gacgattgac gaagctgcta aacgagcaga agaacttcgt acaagactga accaatggtc
     7801 tcgcgaatac tacgtagaag ataaaccaac agtggaagac tatgtttatg ataaagaata
     7861 cgcagaatta gtagcaattg aggaacaata tcctgatttg atcacgtctg attctcccac
     7921 tcaacgagta ggcggaaaag tattagaagg ttttgaaaaa gtcactcatg atattccgct
     7981 ttacagtttg aacgacgttt tcagcaaaga agagctgata gcttttgatc aacgtgtaca
     8041 aaaagcagtg ggacgtgtag ttgactattg ctgtgaacta aaaatagatg gactttccgt
     8101 ttctttgcgt tatgaagatg gaaatttcgt gagaggtgcg actcgcgggg atggaactgt
     8161 aggagaaaac atcactgaaa acctaaaaac tgtccggtcg gtcccaatca aattaaaaga
     8221 accaatgaac atcgaagtaa gaggcgaatg tttcatgcca aaacggtcat ttgtccaatt
     8281 gaaccaagac agagaagcag agggaaaaga tatctttgcg aatcctcgga acgccgcagc
     8341 tggttctttg cgtcagttag attctaaaat cacagcgaaa agaaatctgg acactttttt
     8401 atacacagta gctgatttcg gtcctatgca agcaaaaacc cagtatgatg cgctagaaga
     8461 gctagaaaaa attggattcc acacgaatcg ggaaaaacgt ctatgtcatt cgattgatga
     8521 agtgtggtca tacattgaag aatatcatga caaacgtgtg gatttgccct atgaaatcga
     8581 tggaatcgtc atcaaagtga atgaattctc gctacaagat caacttggtt ttactgtcaa
     8641 agcaccgaga tgggcagcag cttacaagtt tccgccagaa gaagttgaaa cgctaatcga
     8701 aaacatcgaa tggacagtcg gtcgaacagg ggtagtaacg ccaacagcta tcatgacacc
     8761 agtccgagta gcaggaacga cagtaagccg tgccagcctg cataacggtg attatattaa
     8821 gttgaaggat atccgcttga aagatacagt tctgatttat aaagcgggag acatcatccc
     8881 agaagtttcg caagtcgtgt tagataaacg acctaaagac agcgaagaat accaattgcc
     8941 tacacattgt ccagtttgtg gaagtgaact tgtccatttg gatgaagaag tagccttacg
     9001 gtgcatcaat cctaaatgcc ctgctcaaat gaaagaaggt ttgaaccact ttgtttcaag
     9061 aaatgcgatg aatatcgatg ggttggggcc gcgcgtacta gaacagatgt atgataaaaa
     9121 actagtagca gatgttgctg atctttacaa attgacagaa gaagagttat tgacgttgga
     9181 caaaataaaa gaaaaatcag caaataatat tttgacagca atcgacaaca gcaaagataa
     9241 ttcggtcgag cggttgattt tcgggttagg aatccgacat gtcggagcga aagcagcaaa
     9301 aattttagca gaacatttcg gtgatctaga aacattgagt aaaagtgact atgagtcgat
     9361 tattgcgctt gatacgatcg gtgatatcat tgccgacagt gtagtcactt atttcagtaa
     9421 cgaagaagtc catgaattga tgaatgaatt aaaacaagca ggcgtcaatt tcgaatacaa
     9481 agggcttcga aatgcccaat tgcaagaagt cgagtctcct tttaaagaaa aaacagtagt
     9541 ccttactggt aaactaacac gatttactcg tgaagaagca aaagagacga tcgaaaatct
     9601 tggcgggaaa gtgactggta gcgtatcgaa aaaaacggat atcgtggtag caggagaaga
     9661 tgctggcagc aagctgacca aagcacaaga attaggaatc gaagtttgga cagaagatca
     9721 aatggcagat gcattggcta aaagtcgctc agtagaagaa tgaaaaatga gttttttcag
     9781 acaaatttca ataaacaatt tcagacacgg cactcttgtg ttaaaataga gagcaaagtt
     9841 tttaactcgt ttagaaagaa ggggtagtat ggcgattagt gaagaacaag tgaaacacgt
     9901 tgccaaatta gcaaaacttg cgttcgcaga tgaagaactt gcaagtttta ccgaccaatt
     9961 aggtaaaatc atcgatatgg ttgaaatgct ggaagaagta gatacagaag gtgttccatt
    10021 tacgtcgaat gtggttgaaa cagtaaacgt gatgcgacaa gatcgggcgc aagctggatg
    10081 gaaccgcgat gaattattga aaaatgtgcc ggaaacagaa gacggcttta tcaaagtacc
    10141 cgcaatcatc gataatgggg aggctggcgc ataatgacag aattacacaa tcgttctcta
    10201 gaagaactgc atcatatgct tgtatcgaaa gaaatcactg tacaagattt gacaaaagca
    10261 acatttgaac gaatcaaaga aacagagcct gaggtcgaag cattcattac attgaacgaa
    10321 gaaaaagcat tggaacaggc aaaagcttta gatgaaaaag ggattgatga aagcaacgtc
    10381 cttgctggta ttccaattgg tatcaaggat aatatcgtaa caaaagatat cttgacaacc
    10441 gctgcatcta agatccttta caattttgaa ccaatttatg atgcaacggt catggataaa
    10501 atctatcaag cagatatgat ccctgttggt aagctaaaca tggatgaatt tgctatgggt
    10561 ggaagtacag aaacttctta tttcaagaaa acaaaaaatg cttgggaccc aacaaaagta
    10621 cctggtggct cttctggtgg ttctgcagca gcagttgcag ctggacaaat tccagtttct
    10681 ttaggtagtg atactggggg aagtatccgc caaccagcag cttttaacgg catcgtaggg
    10741 atgaaaccta cttatggccg tgtctcacgt tttggtttga ttgcctttgc ttcatctctt
    10801 gaccaaatcg gaccaatgac ccgtacggtg aaagataatg cattagcatt gacagctatc
    10861 agcggttatg acgaaaaaga cggtacttct tcaggcgttt cagttcctga ttttgcagag
    10921 ggactgacag gcgacatcaa aggaatgaag attgctttac ctaaagaata cttaggcgaa
    10981 ggtgtggccc ctggcgtaaa agaagctgta ttgaaagcag cagaaacatt ccgttcatta
    11041 ggtgctgttg tggaagaagt cagcttgcct cattcaaaat acggtgtggc tgtttattat
    11101 atcattgcct catcagaagc aagttccaac ttgcaacgat tcgatggtat ccgctacggt
    11161 tatcgctcag aaaatgtgaa atcattagat gatgtatatg tcaactcacg ttcagaagga
    11221 ttcggtgaag aagtaaaacg ccgtatcatg ttaggtacat tctccttaag tgctggttat
    11281 tatgacgctt acttcaaaaa agcgggacaa gtccgtacat tgatcaaacg agattttgaa
    11341 aaagtctttg cagattatga cttgatcatc gggccatctt caccaacagt tgcattcgga
    11401 atcggtgaga acattaacga tccaatcaca atgtacatga gtgacatctt aacgatccct
    11461 gtcaacttgg ctggacttcc aggtatgtct gtaccggcag gtttatcaga aggattacca
    11521 gttggattgc agatcatcgg taattatttt gatgaaaaaa cgatgtatca agcggcatat
    11581 gcgtttgaac aagcaacaga attccataaa caacaaccag ctattttagg agggaaagac
    11641 gaatgaattt tgaaacagtc attggactag aagtccacgt cgagttaaaa accaactcaa
    11701 aaattttctc tccagcgcca gctcattttg gtgcagaacc aaacagcaat acaaacgtga
    11761 tcgactgggg ttatccaggc gtgctgccag taatgaataa agctgcgctg gaattcggga
    11821 tgaaagcagc tttggcatta aactgtgaaa tctctaaaga tactcatttt gaccggaaaa
    11881 attactttta cccagataat ccaaaagcat atcaaatttc ccaatttgat caaccaatcg
    11941 gtcatgacgg atggatcgaa atcgaagtag aaggtaaaaa gaaaaaaatc cggatcgaac
    12001 gtgtccattt agaagaagat gctggaaaaa acatgcatgg tattggcggt tattcttacg
    12061 ttgatttgaa ccgacaaggc acgcctttga tcgaaatcgt ttctgaagct gatatgcgtt
    12121 caccagaaga agcatacgct tatctagaag cattgcgatc aattatccaa tttacagaag
    12181 tcagcgatgt gaaaatggaa gaaggctcta tgcgctgtga tgccaatatt tctcttcgcc
    12241 cttatggaca agaagagttc ggtacgaaag cagaattgaa aaacttgaac tctttatctt
    12301 tcgtgaaaaa aggtttagct tttgaagaaa aacgtcaagc aaaagttctt ctttcaggag
    12361 gagaaatcca acaagaaaca cgccgttttg acgaaacaac gaataaaaca gttttgatgc
    12421 gtgtcaaaga agggtctagt gattatcgtt acttcccaga gcctgatatc ccgaaattcg
    12481 tgatcgatga cgaatggatt caaaaagtaa aagctagttt gcctgagatg ccagcttctc
    12541 gtcgcgaacg ctatatccgt gagtttggac ttccagagta tgacgcaatg gtattgacac
    12601 ttacaaaaga aatgtctgat ttttttgaag ctgttttgga aaatggtgca gatgccaagc
    12661 aagcatctaa ctggttgatg ggtgaagttt ctgcttactt gaacagtgag aaactggaat
    12721 tagctgagac aaaattgaca cctgagaact tagcaggaat gattcgtttg atcgccgatg
    12781 gaacgatcag ctcaaaaatc gccaaaaaag tcttccgtga attgatcaca aatggcggag
    12841 aagcccaaga agtagttgaa aaaaatggtt gggtgcaatt atctgatccg aacaaactac
    12901 tgccaatcat taatgaaatt ttggataaca accaacaatc tgtcgatgat ttcaaaaatg
    12961 gaaaagatcg tgcggttggc ttcctagtcg gtcaaatcat gaaagcaact aaaggacaag
    13021 caaatcctgg tgtcgtgaat aagctactta aagatgaatt agcgaaacga tagaggctga
    13081 aaatgaaaaa agcacgcgta atttataacc ccacctcggg aaaagaatta ttgaaaaaaa
    13141 atttggcgga tatcctacag gcattagaag aagcaggatt tgaagccagc gcctatgcaa
    13201 ctacaccaga accagattca gccaagaatg aagcatgtag agtcgcagaa ctaggttttg
    13261 atctagtagt ggcagctggt ggcgatggca caatcaacga agtggttaat ggaatcgctc
    13321 cgcttgaaca ccgtcctaaa atggccatca tcccagctgg tacaacgaac gactatgctc
    13381 gagcattaaa aattcctaga gataatatta aagcagcagc agaagtgata aaaaaacacc
    13441 aaaccgtaaa aatggacatc ggcctttctg ataacagtta cttcatcaat atcgctgcag
    13501 gtggctattt gacagaattg acttatgaag ttccatcaga actaaagagt atttttggtt
    13561 atcttgctta tcttgcgaaa ggagcagaga tgctccctcg agtaaagcca atcaaaatgc
    13621 gcttgaagta tgatgaaggg gaatatatcg gaaatgcttc gatgtttttc ctagggctga
    13681 cgaactctgt tggaggattc gaacagatcg cgccagatgc caaattggat gatgggaagt
    13741 tttctttaat cattgtgaag acagcaaata tctttgagat cttgcatatt gcagcactca
    13801 tgctgaatgg cggtcgacat gtcgatgatc caagaataat ctataccaaa acaagcagtt
    13861 tgttcgttga aacagaagat gatatcaatc gaccagtaat gatcaactta gatggtgaat
    13921 acggcgggga agcaccgatg catttccaaa atcttcatca acacattgag ttctttgcca
    13981 ataccgatca gattcctgat gaagcaatca caggtacaga tgaagaagaa ttagaggttg
    14041 tcagcaaaga attcgtaaaa gaagtcgaac gtttaacgga tgaagatatc gacggtgacg
    14101 gaaaaatagc agacgaaaca cctaatcaaa agaattaatc aaaagccggt gctgaacgta
    14161 gtaaggtcag tgccggtttt agttgtgtaa ttagctgatt tatagtagag ttcagtagga
    14221 ttgaaaaata ggcttaaagg agcaaaatat gacagaacag caaatagtaa agaaaaacga
    14281 acgatataca gtcgaaatcg tcgacttgag ttatgaagga atgggggtag caaaaatcga
    14341 tggttatcca ttatttatcg aaaatgcact ccctggagaa gaagtagaaa tactagtagt
    14401 aaaagcaggc aataagtttg gatatggaaa agtcgaaaca tttattacac aatcacctga
    14461 tcgtcagcct gtagaaaaca atgcattact tcgcataggt atcgcacctt tagcccattt
    14521 gaagtatgaa caacagcttg tcttcaaaca aaaacaagta gagcatgtaa tggcgaagat
    14581 tgctaagatg ccagaagttc ctgtacttcc aacggtcgga atggcagaac cattcggcta
    14641 ccgtaataaa gcacagatcc ctgttcgccg tatcgatgga cagttagcca ccggttttta
    14701 caaaaagaat agccatgatc tagtccctat cgaagatttt tatatccaag atcctgtcat
    14761 cgatcaagca atcaaagtag tcagagatat cttgcaacgg ttccaagtac gaggatataa
    14821 tgaagaaaaa aatgaaggac agcttcgtca catcattatc cgtcgtggtc attattccca
    14881 tgaaatgatg gtcgttttag ttactcgtaa agaaaaattc tttaaatcca aggaaattgc
    14941 tgaagctatt catgaagaat tgccagaagt ggtttcggtc atccaaaata tcaatgaaga
    15001 aaaaacaaat gtgattttag gagacaaaga aaaagtttta tatggcagag actatatcga
    15061 agatcagcta ttaggaaaaa cctatcgaat ctcagcaaag tcattttatc aagtaaatac
    15121 tcaacaagca gagtatctgt accaaacagc catcgagttt gccgagttag ataaaaaaga
    15181 cattgtgatc gacgcttatt caggaatagg tacgattggc ttgtctttag cagacagagt
    15241 ggataaggtg tatgggatgg aagttattcc agaagcaatt gaagatgcac agttcaatgc
    15301 tttaaataat aaaatcgaaa atgttcatta tgaagtcgga aaagcagaaa aagttatgaa
    15361 aaaatggcaa gaaaaaggga tcaaaccatc tgtgatcgta gttgaccctc cacgaaaagg
    15421 attagatcat cattttattg aatcagcaat cgaaatgtca ccagaacgaa tcgtctatat
    15481 ctcatgtaat ccggcaactt ttgcaagaga tgctaagtta tttgcagaag caggttatgc
    15541 cgtgaagaaa gttcagcctg tagatctttt cccgcaaacc catcatatcg aattagtcgc
    15601 ttcattcgtt aaaggagaat aaaaatggac caaaaagaaa aaatgaaaga attgatcaaa
    15661 aagaaacaag gtggaggccg atccaaacaa atggaaacac ctaagaatga tcgtaaaaac
    15721 atgagaaaag gccctaaaat ctttaataaa tagacgtgct aaa
//
LOCUS       DACESX010000058        15442 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.058, whole genome shotgun sequence.
ACCESSION   DACESX010000058 DACESX010000000
VERSION     DACESX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.058"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="IXF88_001549"
     CDS             complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="IXF88_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1508285.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1197..3038)
                     /locus_tag="IXF88_001550"
     CDS             complement(1197..3038)
                     /locus_tag="IXF88_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1508286.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQKLVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARQTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3220..5613)
                     /locus_tag="IXF88_001551"
     CDS             complement(3220..5613)
                     /locus_tag="IXF88_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1508287.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5720..6580)
                     /locus_tag="IXF88_001552"
     CDS             complement(5720..6580)
                     /locus_tag="IXF88_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1508288.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6639..7271)
                     /locus_tag="IXF88_001553"
                     /pseudo
     CDS             complement(6639..7271)
                     /locus_tag="IXF88_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
     gene            7480..8199
                     /locus_tag="IXF88_001554"
     CDS             7480..8199
                     /locus_tag="IXF88_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1508289.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8225..10816
                     /gene="mprF"
                     /locus_tag="IXF88_001555"
     CDS             8225..10816
                     /gene="mprF"
                     /locus_tag="IXF88_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1508290.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="IXF88_001556"
     CDS             complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="IXF88_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1508291.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12559..12915
                     /locus_tag="IXF88_001557"
     CDS             12559..12915
                     /locus_tag="IXF88_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1508292.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(13156..14160)
                     /locus_tag="IXF88_001558"
     CDS             complement(13156..14160)
                     /locus_tag="IXF88_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1508293.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            14326..15177
                     /locus_tag="IXF88_001559"
     CDS             14326..15177
                     /locus_tag="IXF88_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1508294.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            15194..15439
                     /locus_tag="IXF88_001560"
     CDS             15194..15439
                     /locus_tag="IXF88_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508295.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         4483 a         3208 c         2729 g         5022 t
ORIGIN      
        1 tttttttctt ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca
       61 cgcttattct aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa
      121 tgctttagcc tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc
      181 ttccaatgtt ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc
      241 ccgatcagtc aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc
      301 gtgttctgct ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc
      361 ttcttcaccg attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg
      421 gactttttct gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc
      481 aaggtcttgc agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg
      541 aactggaata cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat
      601 ccaccatgta gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc
      661 cttcatcagt cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac
      721 ataacgttta gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc
      781 ttcttggtcg ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag
      841 ggagacacgg ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt
      901 tggcgcagaa agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct
      961 gtgaattgat ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg
     1021 gatcaatttg tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa
     1081 atcatcatat acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc
     1141 ttcatacgtt tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat
     1201 ttggcttgtt ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc
     1261 tgtttgtttc cagtttgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca
     1321 gctaagccag atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct
     1381 tctgccaccg gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa
     1441 aagtctaaaa actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa
     1501 aaataaactt ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta
     1561 cggacagaaa cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc
     1621 gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc
     1681 tgaggttctt gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc
     1741 gtctctctgc cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt
     1801 ggtgaaacaa ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt
     1861 tcattatcta aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg
     1921 cgcccatgag ccactaattt ttggaaagat gcttctccat acttacggag atattcatca
     1981 ggatcaagct tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc
     2041 aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc
     2101 aattctttcg tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata
     2161 gaagctaatc cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg
     2221 aacaacaaaa tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa
     2281 acttgtcttt tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt
     2341 tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa
     2401 ttacgaagtg gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc
     2461 gtgacaaaca atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact
     2521 tgctgaagaa aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct
     2581 aaagacaacc cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat
     2641 agcatgtgat gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca
     2701 gattcaggat tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt
     2761 tcgaagtctg ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga
     2821 aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct
     2881 gcaacagaaa aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta
     2941 ccagactttt tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt
     3001 actgtttcga taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg
     3061 atatcttttg ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa
     3121 ctgaaatcag tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat
     3181 tgctttttca agtataacac atttctatct cattaagcat caatttgcta ctcttttcag
     3241 taactgattt tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat
     3301 gaatacaaag aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg
     3361 gaacagcatt gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa
     3421 tgggttgaag ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta
     3481 atacatcaaa gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac
     3541 cgcaatgata ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg
     3601 caaataacgg taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata
     3661 cccttcaatc gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc
     3721 tgtaacggca catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc
     3781 tgagactttc gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt
     3841 agtaaaatca gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc
     3901 tgccatagca atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc
     3961 tgtcattgca accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg
     4021 cggactcact cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc
     4081 agtaatttga gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat
     4141 attggagaca gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg
     4201 aagataagct agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc
     4261 aaccggctca acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa
     4321 cataagtacg cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc
     4381 ttccaccaaa cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc
     4441 gccccatttg cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag
     4501 ctcaaaataa tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata
     4561 cgtccccata atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg
     4621 gatactcatt ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg
     4681 ggccagcgtt tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt
     4741 tacccctgtc gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa
     4801 caaagctgct gcagatgcaa taacagaaat tttgatagaa gcatcgcgga tgaacagtgc
     4861 ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt
     4921 gcgaatcgaa tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa
     4981 gcggacagca taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt
     5041 caaaaagctt ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc
     5101 tgtcaaaaga gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat
     5161 ctgttctcct gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga
     5221 ttgttcttta ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg
     5281 tacaagattt cttgcatgta tttcttgata aattccaata acgatattga cgacaataat
     5341 tgctaaatac gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa
     5401 gttcagaaaa ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga
     5461 tgcatcttcc tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg
     5521 tgtaagtcca ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg
     5581 tttaagtcta gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa
     5641 cacacgaatg attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt
     5701 ttttattatc aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta
     5761 gtgctgtacc ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg
     5821 cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga
     5881 atagcttgct atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac
     5941 aaagaatccc accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt
     6001 cagccatcgc tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga
     6061 cgatcatacc caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga
     6121 cttgcccgtc aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa
     6181 caaccatgat gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg
     6241 caggaacagt tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac
     6301 ctaataaaaa actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa
     6361 caggcagaaa aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa
     6421 tcagccgctc atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga
     6481 taaatcctga accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt
     6541 accccacttc ttgattttcc attttttgcc ttttttccac tatttgcgca ctccttcttt
     6601 catttaattt tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag
     6661 gtctctatta ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta
     6721 tgatagagat agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc
     6781 ccttcctcat ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct
     6841 actgtgtttt ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat
     6901 gcttggacag ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc
     6961 aaatatttga caccttgatc aatactgtca gctggattat caatgatctc agcttctcca
     7021 tgcagacttt ccgagctcta catgagatct gtcccttttc cttttgattc ggtcaatatt
     7081 atcgccttga tcaaattctc gtagttttcc atatgataat tttttgcagc tgttgttatt
     7141 tctatttgat agctttccac tcgttttacc gaacgataag ttcttcctcc taaaaacaac
     7201 accgcaccaa taaccgtcag aatgatcatg taaaaaagaa tacggataat agataatcgt
     7261 tttcgcttca tttcatcacc tagttatttt gacatctttt tttatcttac tatttcttta
     7321 tcttcttggc tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat gaattttcct
     7381 gtattttttg acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg
     7441 actgatataa tcgttatact ttacacagta ggtgattatg tgaaaaaaga aaagaaatat
     7501 gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga gcggtcaagg cttccctctc
     7561 tttttactgc atggcaatga cggcagcggc cgcttttttt cagaacaggt ccctgtactc
     7621 gaacgctact ataccgtgta tctagtagat agccgaggac atggtcgttc gacaaacgaa
     7681 gcatctatgt tgaattttca gttaatggcc gaggatctga atacgatcat gttgctggaa
     7741 aaaatcgatc aggcagattt tcttggattt agtgatggag caaatctagc actggtattt
     7801 gccagcagct tccctaaaaa agttcatcga ttgattttaa attctggaaa cacgcttgtt
     7861 aaaggcgttc gtttttctgc cagagtcatc agtaatattc attatgcatg ggtatggtta
     7921 ctctctttat ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact tttgctgcat
     7981 gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact cccccacttt gatcatcgtg
     8041 ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata tagctaaaac tataccaaaa
     8101 gcttcctttg tgcttgtgaa agaacaagga catgaattag ctagaaaaga tcctgaacgc
     8161 ttcaatcgag aagttcttca atttctttct gaaacataac tattaggaat agaaggtgat
     8221 ttatttgtta aaaaatacca tacaatggct gaaaaaccac ctaggactgt tcaaaacaat
     8281 ctttttgatt tctgtcattg tcattattgt cggtgaattg atgtccatcg gtaaaactct
     8341 atctatccag caattaggag aaacgtttgc cactatacct gtatggaaaa gcggactaat
     8401 gctattgatt ggtcttatct ctgtgttacc tatggttggt tatgatatta ttttgaatcg
     8461 gatgcttgaa caaaagcaga atcctcgtta cctgtttgag acaagctggc tgatcaatac
     8521 gatcaacaat attgccggtt tcggcgggtt tgttagtgtg gggcttcgct ctgaactata
     8581 tggacaaaag aaagagagca gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt
     8641 gatggctgga ctttctattt acagtctgct ttctttttta cttgtagtct tcacgccagt
     8701 tgctcctttt ttgaagcaat attggatatg gttgatcggc ggcggtctat attttcctgt
     8761 cgtcttttta tttactactt tcaaaaagaa aggattcctt ggtggtacct cacttaaaac
     8821 tcaaggacag ctattgctag tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct
     8881 ttctgttggt tatttaatgg aaatccggat tgatctgtgg caaacaattc cattattcat
     8941 tgctgcttca gtgataggaa tcgtgtctat gattccagga gaaattggga gtttcgatgt
     9001 aatgatgatt atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat
     9061 cttgctctat cgcctgttct actacatcat tccgtttttg attggaatcg tctttttctt
     9121 caaaaatatc ggttccactt ttgatcagcg ttattctggt atccctaaac aattagcaac
     9181 agaaattgcg cataaaatcg tggttgtctt attatacttt tctggaatca tgctagtact
     9241 ttctgccact atcccgcaag cttttacaga gtttcgctgg cttcatagtt tgaacccatt
     9301 gaaatttcat tttattatcc aatttcctag tatactattg ggatttcttt taatcattat
     9361 gggcagaggg atcgcagcac gtgtgaaacg agcatatttg cctacaatct tcttgattgt
     9421 tctagcttta ctttatgttt tattaagtga ttttagtttc acaccggtgg ttttcttgtc
     9481 tattctgctt ctgatcattc ttgcttctaa aaacgaatta tttcgggaac aattgatcta
     9541 ttcatgggag tggcgtacga tcgatggaat cattattggt gcactgtcct tgctttacat
     9601 cgttattggg gtatacaatc tgccagattt ccctcatcgg cgtcaccatt ttatttcctt
     9661 tttcttattt ccttctgaaa agatttggtt ttcgggattg ttggccatca tcgctgttag
     9721 tttcatgatc gttctatttg ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc
     9781 ctttaatgaa gaaaaggccc tgaagatcct tacaacttat ggaggaaact ccgatagtca
     9841 attgatcttc ttaaaagata agcggatgtt tgcctatgaa aaagacggtg aaccaactgt
     9901 tcttctccaa ttcgcttgct tcaataataa atgtattgta atgggtgatc cttctggtaa
     9961 gaaagaagat tttccagaag caatcgaagc ttttatcgaa gaaacagatc gactatgcta
    10021 tttaccagtt ttctacgaaa caagcgaaga gattgttatg attttgcatg aattcggcta
    10081 cgatttcatt aagatggggg aagaagctta tgtagacctg aatagtttta caacttcagg
    10141 gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc gaacgagaag gatttacatt
    10201 tgatgtctta caaccaccat tttctactga gcaaatgagt actttcaaaa atatctctga
    10261 taattggttg ggttcacgaa aggaaaaagg gttttcttta ggctttttct ctgaagatta
    10321 tctacaaaga gcgcctattg cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc
    10381 aacgatcatg cctacttaca cgaacaataa agtaggaacg attgatctga tgcgatatga
    10441 ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc ttgcatttgt ttgcctatat
    10501 gaaagaagaa catatccaat ggttcaattt agggatggcc cctctttcaa atgtcggaac
    10561 ttccagaaag agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca
    10621 tttttattct ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc
    10681 acgttatact ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat
    10741 tattgataat gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat
    10801 acgaagaaag tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga
    10861 gactgaagta aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa
    10921 agagcggtcc aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta
    10981 ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact
    11041 gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc
    11101 aagtcaacat caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat
    11161 gtgattgttc ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag
    11221 aatggagtat gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt
    11281 gtatgtggag taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt
    11341 tggatccccc gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta
    11401 cggaacattt ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc
    11461 tctacctcat caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct
    11521 cgacctgtaa ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt
    11581 tctggtgttg ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca
    11641 tctggatcgc cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct
    11701 gggaaaccat attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct
    11761 tcatcatcga ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg
    11821 cgtgacaata aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact
    11881 aagattgcac catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt
    11941 cctggtgcat caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta
    12001 ttgatcgtaa tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga
    12061 ggatttgcta ggcctttttt gcctaatact gtagtgatcg cagcagttaa agtagttttc
    12121 ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt
    12181 tcttttgcca taatttacaa acctcctaaa taatatagat cactgaaaaa tttcagctct
    12241 caccttttta ttatagtctt tttttgacta aatcaaaaag aatacgtccg acttactttt
    12301 tgtaagattc gaactaagct tattatacgt caatattggt caaagtcaac taagagcact
    12361 cgtctgtctt atacgcgaaa aaagtacctt ttataaataa aaaaatgtgt tttttgagaa
    12421 aaacttcaag atagtcctta actttctttt gaagcaataa gaacaaaatg aggaaaagtt
    12481 taaaattcag ctgttgccaa caactttcaa tagacgaaat cttctatttc gttaataata
    12541 aggaaagagg tgaagtacat gaaaaaatta attggcgtac ttgtcgtttt aggcatattt
    12601 ctcggcggaa gttttgcagc ttatcattat ttttacggcg gcgaagctta ttatacaaaa
    12661 atcacgacga atggcgaaat gtcagctacc caagcagata acggagagaa attcacgact
    12721 tatacctaca agcaagcggc ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc
    12781 gaagaaagag aacatccatt aaagatgaat gcgtatctta aactcaaagt caatcctcgt
    12841 aaaggtgtat taagctggga agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa
    12901 aaaatcgata attagtaaat ggtcttaaaa ttttattaga taaaaagagg ctgggacaga
    12961 agcgtttaac tccaagaaat aagaaggagt tgcttctgct tccgccgttt atacgtgttt
    13021 agagcgtgag acaaaagtgt tctttacttt tgtctcacgc tctttttttg ttatattcgt
    13081 cttttccgat ccttctcttg acacaattca tttaactgat cactggtact tgaacaccta
    13141 atattctagt catttttata aacgattcaa tacttcatcc acatttttga tcatcacgga
    13201 tatcgtacca tccggattat tcacaaattc aatcaaatct gggttgcgat atacatctac
    13261 tggtacgatc agttcgatcc cgtttgatag cttgaatttt tgttttccaa attttttctc
    13321 agaaatttct tgtacttctt ttacaggagg tgcttctggg acaaatcctt gctcttgtac
    13381 ttcttcttta aaagccattt ttgccgtgat gttttccttg aagacttttt ccgcaatcat
    13441 ctcatgatcc aaccgacctt tttcttctat cgtatcatag acagcttctt tcaaatcagc
    13501 catgatatca aattcttctg tctcaaattt ttttccaaga tgcttggcta ctttttggat
    13561 cacttttaca ttttcttcta aagaaggtgc aggaacactt tcgatcactc ttccagaaaa
    13621 ataaagctct tttttgccag aaaactcata ttttttttca attaattcgt aggtccaatc
    13681 agtcaaattc acagcgaatg cttcatctgc tttttgtgtt ttcgccccta gaattgctcg
    13741 attcaaaatc aagcggttgt cgatccctgc ttcgtctgct tcaacaaagt gagtgtaagc
    13801 ctcacgataa ttgactttca ataaagataa gtacatcact gtatctaatt catacaagac
    13861 aacaaataca tctgcactag gtgcttcttc actttcttgg tagatatcga accaccgctg
    13921 aacgattgct tcacttgctt ccacaaaatt ttcttgagcg attttaagtg attcaacgat
    13981 catgctttct tctgctagtt gtccagtttt tgtttgagca gaagagattt tttggatctt
    14041 cttttgaaga aaatcacgaa tatattcttt tgtcagatcc agctcttttt gtgaaaagac
    14101 tggagaacca gtttctctgt caataatatg cagaattgct ttttttaaat agatatccat
    14161 tttctcattc ccttctgtct tagtttacgg aaaaaaaatc aaaaatacca gaatcaaaat
    14221 cattttcttt taaacaaaaa tttcaagagt agaaacaaaa tggattcttt ttttcgaaaa
    14281 atgcgctaca cttatagttg aacgaataaa aaaggagctg aaagtatgaa ttcattaaca
    14341 agcacttatc gtctatcaaa tggttacgaa atcccggtag taggattcgg cacatggcaa
    14401 acacctgatg gggatgtcgc tgtttcctct gttaaagaag cgctggctgc aggttatcgc
    14461 catatcgata ctgcccaagg atacaagaat gaagaaagcg tcggccaagc gattaaggaa
    14521 agtggaattc ctagagaaga gatattcttg acaacaaaat tatggaatgc aaaccacagc
    14581 tatgaacttg tgatgtcttc ttttgaagaa tcattgaaga agctccaaac agattattta
    14641 gatctgtttt tgatccactg gccaaaccct gttgcttttc gtgataattg ggaacaagca
    14701 aatgcagata catggcgagc atttgaagaa ctgtatgaag caggtaaaat caaagcaatc
    14761 ggtgtgagca atttcttacc ccaccatcta gatacactag caaaaacagc aaaaattatg
    14821 ccgatggtca atcaagtttt ccttgcacca ggcgaattac agacaactgt agttgagtat
    14881 gcaaaaaaac atgatatgat cctcgaagca tatagtccat taggcactgg taaaatcttc
    14941 gatgtgcctg aaatgaaaca aatcgctgaa gcacatgaca agacgattgc acaagttgct
    15001 ttgcgttggt cgctgcaaca cgaattcctt cctcttccta agtcagtgac accaagcaga
    15061 atcaaagaaa atactgaatt atttgatttt gaattaacag aagaagaaat gaaacaaatc
    15121 gatcagttag atggtgtagt cggcaaagca aaagacccag atactaccca attttaagtc
    15181 gaaaggtgat tttatggatc caatcgaacg cttgaattct ctctcggaag aagttaccca
    15241 gacttttcat tccgattttg tctttttgat cgatgcagag aaaatccaac attttcctgc
    15301 aagaaactgg acacatgatc aaatcatcga agaactgaaa aaacggttcg atcattcgct
    15361 aatggtcaaa ccctggcacg aacatgaagt catttattca cctgaactac ctgtttttgc
    15421 cttgataccg aaaaaataga aa
//
LOCUS       DACESX010000059        15344 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.059, whole genome shotgun sequence.
ACCESSION   DACESX010000059 DACESX010000000
VERSION     DACESX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.059"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            73..2133
                     /locus_tag="IXF88_001561"
     CDS             73..2133
                     /locus_tag="IXF88_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508296.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2216..2785
                     /locus_tag="IXF88_001562"
     CDS             2216..2785
                     /locus_tag="IXF88_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1508297.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2960..3766)
                     /locus_tag="IXF88_001563"
     CDS             complement(2960..3766)
                     /locus_tag="IXF88_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508298.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3904..5418
                     /locus_tag="IXF88_001564"
     CDS             3904..5418
                     /locus_tag="IXF88_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1508299.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5821..6804)
                     /locus_tag="IXF88_001565"
     CDS             complement(5821..6804)
                     /locus_tag="IXF88_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ1508300.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6809..7456)
                     /locus_tag="IXF88_001566"
     CDS             complement(6809..7456)
                     /locus_tag="IXF88_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1508301.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7541..8173)
                     /locus_tag="IXF88_001567"
     CDS             complement(7541..8173)
                     /locus_tag="IXF88_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1508302.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8189..8740)
                     /gene="coaC"
                     /locus_tag="IXF88_001568"
     CDS             complement(8189..8740)
                     /gene="coaC"
                     /locus_tag="IXF88_001568"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1508303.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8737..9474)
                     /locus_tag="IXF88_001569"
     CDS             complement(8737..9474)
                     /locus_tag="IXF88_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1508304.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9471..10460)
                     /locus_tag="IXF88_001570"
     CDS             complement(9471..10460)
                     /locus_tag="IXF88_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1508305.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10718..11260
                     /locus_tag="IXF88_001571"
     CDS             10718..11260
                     /locus_tag="IXF88_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1508306.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11277..12362
                     /locus_tag="IXF88_001572"
     CDS             11277..12362
                     /locus_tag="IXF88_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508307.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12374..13180
                     /locus_tag="IXF88_001573"
     CDS             12374..13180
                     /locus_tag="IXF88_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508308.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13181..14008
                     /locus_tag="IXF88_001574"
     CDS             13181..14008
                     /locus_tag="IXF88_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508309.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14005..15078
                     /locus_tag="IXF88_001575"
     CDS             14005..15078
                     /locus_tag="IXF88_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1508310.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4929 a         2773 c         3134 g         4508 t
ORIGIN      
        1 tttatataat gaaatcatct tatgaaaatt actacttcat gtttagacaa gagtagggag
       61 gactaagaat aaatgtgttc aaaaagacgt tcaaaacaac taaataaaca cgcttgtcgt
      121 ttagataagg aacggtcgtg ttctgaaaaa cactacgtta agtttatgat gcgcacgatg
      181 ctgatcagtt tatctgtagg tgtctatatg catgtacccg ttcaggcaac gacacaaacc
      241 atcaaggtcg tacgtacctc tgtttctgag accactgatt cccctatcac tattactcat
      301 actttcactg atgcaaatgg aaaacagcta cttcctgcat tgacttccta taataaagtc
      361 agttatcctc caacgataaa ccactactgg ttaattccta acaaaacatt atggacatcg
      421 gaaaccggaa gcagtgtgcc gctttctatc ttttccgata tgactgatcc tacaaaaggt
      481 gatcagttaa aacagatcac tgatattttg aatcatgctt tgacagatat aaaggacggt
      541 ggaagcgcac aagtttctta tgtatatgaa aaagattatt cacagtttga aagaaagaaa
      601 atcgatgttc tggcagggac aaaagtttcg atatctgatc tggtagaaag gctgaagaac
      661 gcagatggaa cacaaggaga tatgtccaaa gtaatttcaa acaatgggaa tatcgtggat
      721 acgactaata caggaacatc tactatttcc ttgtcctatt ttgatccaat tgctttaaaa
      781 acaatgactg catctgctgt gatccatgtt cgtgctgatc aaacagctat caaaggaaaa
      841 gatcttcact taagaacagg agagagttac gcccctaaag atcttgttga atatgtaaca
      901 aaaagcgatg gaacttcagg aaataaagaa gaaatcaaaa tgaacgggac agtagatact
      961 acaactccgg gagaatatcc cgttgtatta acttatatag actcgaaaac atcaaatgtg
     1021 gcagaaacaa cagctgtagt ttatgtagaa gcatctgctg cttcatcgac tgtcatcact
     1081 cattttatag atagtcaaac aaataaagaa cttcaagcag aaacacatat gagtagtgta
     1141 aatcgtattc ctgatttgcg cctacctgct gaattttctg atcgagtgcc aaatatagat
     1201 ttatcgactt atgaacaaaa tgggaaagtc acttctattt ctgattggat gcaagcaaac
     1261 ggattagaca gaaaaagtca ctggtcggag atccttgaag tacttcactc agaactgatg
     1321 aataatagga agcaatcagg aggggaaatt acctttactt atgtttatat accagacaat
     1381 cgttcattag aaggtcatga tcttattgtt catccaaacg aaaaaataaa caaagaagag
     1441 ttgatccaat cagcaacaaa ttccttaggt aaacccgtac cgttaaagga tgtatcggtt
     1501 acaatcaatg aagtaccatt ggaggatggt tatttgacca aacaaacagg tgattttacg
     1561 attatgtata gttatcatga cccgtattca ctccaagaac tccatgctca gtcactatta
     1621 catgttttac cattaaacaa acagatagat caaaaagaac aaaaaagaca agaagaaagc
     1681 ttgtcagaaa aaaaacaaca aatggctcat gcagtttctc caacagggaa agtaaaacaa
     1741 acaagaagcc tagagttagc aagaatatct agaaaaatct cagaatctaa taaaacgata
     1801 aggcaagaag ctcatttggc cccacaagtt tctggattgg aaaatgaaat tttagagcct
     1861 aaaataaaac aacaatcaga aaaagaattt gttgctaaac agtcggaaga tcagaaggaa
     1921 tcagatgacc agaaggaaac gaagaaaaaa cgcttgtcaa ctattgattc caccgatgag
     1981 catctgcagc cgcaagtacc acttcctagt ccgggaacac ccgcacctgg tggaggtatt
     2041 ccttcttctg ctttaggaga tttcatgcga ggtatggcag gaacatgggt atacgctaat
     2101 cgaaatgaat ttgacgcaga tgactggttc tgatttatat ctctatctgt tatagtcaaa
     2161 taagtagctg agtcatatgt tatttggcat aatggagacg aggagggata gaaaaatgtt
     2221 agataaaacg ttaccttacg cagaaatctg gatgacaaga ccacttcaac aaaatgttcc
     2281 aatcacgcct ttagcggaag gttttgaatt tcgattttat caaaaagaag atgaatctgc
     2341 ttgggcggag attgagactt ccgttgggga atttcaaacg acagatgaag cacttgagta
     2401 ttttcaacaa aactttgctc cttatcctga agaacttgaa agaaggatgt tttttgtaga
     2461 gacaaaaaaa ggagaaaaag tagcaacctg taccgcttgg cgaaaaaaat caagagagca
     2521 agtgtatccg gtttttcatt ggttagcagt gaaaccagat taccaaggaa aagggctcgc
     2581 aaaagcattg actgccagag tcttgcaaga atttcctacc ctccatacgg acggaccaat
     2641 ctatcttcac acacagacat ggagccatca ggcgattgct ctttataaaa aaatgggttt
     2701 tacttttatt gctgaaaatc tagacggcac acctaatcca gattatgaaa aagttatgca
     2761 ggttttaagt aaattagata aataacgaaa tggtgaaata agtcctcagt attgggataa
     2821 aaaacgaaat agtcattcct catatcttac ttttttgagt taatactatc ggataactaa
     2881 tggcatgcca tacattcaaa aaagaagggg cagtgacaaa agtattgtca ctgccccttc
     2941 tcttgttttt tagcatgttt tatcgtttga tttttttagg aataaaattc ttgatgaacg
     3001 taaatttttg gtaatcgttt cgactataga ggtaaccaaa tacactgaaa acaactgctc
     3061 caataaaatt aacaaataaa tccttcatag tatccatgat tcccacgtct aagtaaccat
     3121 tttggatgat tgttttatct gttttaccat ctgcattttg actttcaatg attgtccgag
     3181 tgatatctgg aacatgatag acagtgtttc cgtctttact taactccaca ctattgattt
     3241 gctggatgat cgtatctttt tgcatatcga attgcagtaa tttatctgct gaaaattcca
     3301 caaactccca cataacacca actgtcatcg aaaaacagaa ggaaacaact gctagtaata
     3361 aaggagtcaa gttcatggtt tttgtatttt tattgagcaa ataaacaagg gaaaagccga
     3421 cacccccagc taaaaatccg ctgaatgcat gtagaaaagt atcccacatt gaaaaatagc
     3481 cataaaaatc acttaactct cctaaaactg ttgaactaaa aataaaagat aacaaaatga
     3541 tttctagcat atttggaatc tgtatcttta atatcttttc cagaaaataa gggatttcaa
     3601 ataaaattaa tgtcagtaat aaaagcattg tatttcccca gtgttggttc cagacctgat
     3661 ccactagcat aataacgacg atgatacgta ataaaatata gattccgttt gtgattctac
     3721 ctgcagtttt tgtactgttc gtaaaaaagg ttttcaaatg attcataaaa taactccttc
     3781 atgtatatcc ttaattatac taagaagcgt gtgtttacca aagctatatg acttaaagaa
     3841 aaacagagtt gatattgtga aaaaaatcac ttaatatagt ataaggagaa aagggggata
     3901 caaatgaaaa aattagttgc tggtttatta acagccgttg cacttcttgc tatgagcgca
     3961 tgcggcgcag atcagaaaaa tacagcgcag acaagttcta gtacgaagga atctgccgaa
     4021 gtatcatcag gcgcttctga acaagtatat acagatccaa aagaaatgaa agaatcttat
     4081 gatgtcgtga ttattggagc tggtggtgca gggatggcag cagctatcga agcaaaagat
     4141 gctgggttga atccagtaat tttggaaaag atgcctgttg ccggaggaaa cacatctaaa
     4201 tcatcaagcg gtatgaatgc ttcagaaaca aaatttcaaa aagaagaagg aatcaaagac
     4261 tcgaatgatg ccttctatga agaaacgcta aaaggtggtg gaggaacgaa tgataaagaa
     4321 atgcttcgat actttgtcga tcactctgct gatgcggtcg attggctaga tcaaaacggt
     4381 atcaaactgg acaacttaac gatcactgga gggatgagtg aaaagaggac acatcgtccg
     4441 tctgatggct cggctattgg tggatactta gtggaggggt tattgaataa cgtccatgag
     4501 cgggaaatcc ctatttttgt caacgcagat gtgacagaca ttaagaaaaa tggggaaacg
     4561 gtcaatgaag tgactgtaca tgttcaagga gaagaaccaa aagaggtcac cggaaaagca
     4621 atcgtagtta ccacaggcgg ttttggtgcc agcaaagaat ttattgaaaa atatcgtccc
     4681 gatttagctg attatattac gacaaatcaa gaaggatcaa ctggagatgg tatcaccatg
     4741 attgaaaaat taggtggaca aaccgttgat atggataaaa tacaaattca tccaactgtg
     4801 cagcaggatg agggggtctt gattggtgag gttgttcgcg gagaaggagc aatcttggtc
     4861 gatcaagagg ggaaaagatt tgtcaatgaa atggatacaa gggataaagt atctgcagca
     4921 attacagcat tgcctgaaaa atcagcttat ttgatttttg accaaggcgt gcgtgatcgt
     4981 gccacagcta ttgaatttta tgaccaaaaa ggatatgtca caactggaaa gacaatggaa
     5041 gaattagcag aaaaaatcga tttaccaaaa gaaacactag caaaaacagt tgatggatgg
     5101 aatacggatg ttgcggagaa aaaagatgct caattcaacc gtgccacagc catggaacat
     5161 gacttatcgt ctccgaatta ttatgcgatc aaaatcgcac caggaatcca ttacacgatg
     5221 ggcggtgtga aaatcaatac gaacactgaa gtattagata aagaaaacca gccaattagg
     5281 gggttatatg ctgctggtga agtcacaggt ggcttacatg gaaacaaccg gatcggtgga
     5341 aattcagttg cggagatcat tatttttggg cgacaagcgg gcatacaagc agctcgattt
     5401 gcttcggaaa atgaatgaaa tagaacgtaa ataaaaagag gttgtgaaat cagcgttttg
     5461 agctaatacc gagggtcagc tgattgaaca ctgtttagtg aagaggttgt gaaaaagctg
     5521 ccctgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt ttaacttggt
     5581 tggacttatt accgcagatg caagcttttg aacaccgttt attcggaaaa agagcgtggg
     5641 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
     5701 gaaattcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt
     5761 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttgat cagcatgatt
     5821 ttattcagtt atcttcaaaa tgatttttcc gatgtttttg tttgcttcca tatattcatg
     5881 cgcttgtttg actgcgctga attggaatgt acggtcaatg attggccgaa tcttgccatt
     5941 ttctagaaaa ggcccagctt tttgcataaa agtttgtgcg agctctgctt tatactgatc
     6001 acttctaggt gtcaataatg taccaagcag ttggacacgt ttcccaatca atacaccaag
     6061 gtctatctca ggcacgatgg tcccacctag cataccaatc aagacccaac gtccatcaac
     6121 ggctatagaa gctaaattct tttcccagta agaagcgccg atgaaatcca aaataacatc
     6181 gactccccga ccttcagtga tttttcgtac ctcttcttga aagtcttgtt ttttatagtt
     6241 gatcaattca tctgcaccaa gttctttaca aaaggcgagt ttttcttctg atcctgcagt
     6301 aacgatgatt ttcgcgtgag aaagctgtct agctaattgg atcgctgctg ttcccacacc
     6361 acttgctcct gcatggatca agacagtttc gccttcttgt aattttccaa gccagtaaag
     6421 tgtttgataa gcagtcaaaa aaacttctga gattccagca gcagaaacat agtctagcgt
     6481 atcagggaga aggattgcac gatcggcagg catcacagca tattcggcat aaccacctag
     6541 attgactaat ccgtatactt ttgtcccagg agtaaaagtg gagatatcac ttttattctc
     6601 aactacctct ccagctactt cgactcccaa aatagggtag ggaggtttca aagagactga
     6661 ttctcttttc ataatatctg ttcgattgac agctgctgta tggactttga tcagtaattc
     6721 gccttgtttt ggtttaggaa caggcacttc aatttgttct aatacatcta cagaaccagg
     6781 atgcttgatt gaaatggcac gcatggatct attcctcctt tgcccattct tggaatgctg
     6841 tattcaactc tttgctacct tcagaaagta ctagagtcgt acgagaaata tccgtttctt
     6901 ccactagagc ggcaagtaca gcagcaactg tagagcgagt gatcgttttt gatgccttct
     6961 cattaggctt agttaattga atggaagtaa cttcttcatc gttagtcaat gtgactggtt
     7021 gaagaatgac aaaatctaag ttagttcgtt tcagccattc atctgcataa tgtttagtaa
     7081 tataataatc tgtcatggac tcaggccatt ttgctggttc atctgcataa acagcactga
     7141 ccatcagata gcgagagaca tgcgcatttt cagctgcgat cactgttttg attgcgccat
     7201 ccaagtctac ttgcagaaga ttctttcctt gagagccggc agcaaaaatc aatacatcga
     7261 tgcctttgaa tgcttcggtc attttttcca cggaccaagt taaatcaaaa gagacatacg
     7321 aaacattctc tttttttact aatgtttggg aagcaacatc tctcacccct gcaaaaattt
     7381 catgggaact tggagctaaa tcttttatca ggtgtcggcc aatttgtccg tttgcaccta
     7441 caacaaaaat tttcatttgg atcatccttt cgacgtcaaa tctttccatt caatagtaac
     7501 accaaaagag aaaaaaatca gagatcggct acgatgacta tcagttagta agagaagggt
     7561 gcttgaagta tcttaacaag acattgcata ctgctgcagt cgcaagacct gcgacgattc
     7621 cttcaggtat accacttgta aaaataaccg ctagaatagc tgaataaaca gcatttaaat
     7681 ttgttccgat gactttggca taatcttgct ggaataaaaa gtagatcata ttcatcacta
     7741 aaatcgtatt ggtcaatgaa ccggaaagtc cagctaagaa taaagaaacg gaatctgctt
     7801 tttgtttcat tagtttgcgc agacctttat aaacgaaata aggaaccacc ccaatcaaga
     7861 tgcgaggaat aaacgcgatc agcaaggctt tccagcttcc atgttctgtt ccgatgactg
     7921 ggataaacgg agaaaagaca aaagacaaag gtgtttggat aactgtgcta cggatcatgc
     7981 tgatcagtcc aaatgtaccg ccaagaaaag cacctagtct tggtcctaaa acgatcgaag
     8041 caatgattac aggaatatgc atggttgttg cattgattgg accgataggg ataaaaccta
     8101 aaggcgtaac agctaataaa atcaaaatag ctaaaaacat cgcagttaat gtaaagtttt
     8161 ttgtattttt cataagtact ccttttttct aagcatttct ttcattaaga gtcagcatga
     8221 cctgttcgat gatcgtctga atatcagcta aggcaccttt tccaaaatct ccacaagcta
     8281 aaagagattc tcttggttca atttcctgat agccgacaga tctcaatgtt tccagattac
     8341 gctgagtgat cgggttttga tacatattcg tgttcatggc aggggcaatc aattttggtg
     8401 ttcccactgg aagcgccatt gcgactgttg aaagaagatc gtctgcaatg ccgtttgcta
     8461 gcttgccgat aatgtttgct gtagcaggcg cgatgagaaa caagtcagct tgttttgcta
     8521 gttcgatatg attgattacc tgcggatttg attcttgcat gacgtctgta tggactgcgc
     8581 gcttcgacag agattgcaag gttaatggcg tgataaaatg tgtgctgctt ttagacataa
     8641 tcacatctac ctgggcacct aatttgacga attgacttgt aagatcagcg cttttgtaag
     8701 cagaaatact tccagtaacg cctagtaaga tctttttcat tttgattcct ccagtctaat
     8761 tttctcagta atcaatgcag caatctcaga tttactttga gcttcttcca ctgttccatc
     8821 tttagataag agatagccgt ggtgtgtctc gccatggatt tccgtcaaat catttgcgaa
     8881 aataaaatca gttttgttat ttatcagact attttttgcg actgccagaa gagattcctt
     8941 tggtacatct actaatagtt tgaaaccaat gatttttgct tttggctgcc attcacgtag
     9001 ataagaaatg atttttggtg ttttcttcaa aacgagcaca agatgctcgg tgttagaaga
     9061 gatctttgaa acagtggtat ctgtctgttc attttccgca aaccaattgt ccagttcttc
     9121 ttgcgttgaa gaagtgggta ggttttttgc cagctgttct tctgaaaaac taaaagcagg
     9181 agtaaaatca ctgactgcca tactgtggat aatcgcatcg tagtgttctt ttttcgttag
     9241 ttcttctaat tgtaaaaata agtccagtgc agattcgatc gtataaatcg ttatattgga
     9301 gtgtcttttc ggtttcagtg cttgtttcgt agtcacgtaa tcgattgtgg tatccggatt
     9361 ggcagctaaa taatcagcta atgcttgccc taatcgtcct gtcgaatgat ttgtaatagc
     9421 acgtacttga tcgatttttt cagtcgttcc tccagctgtg attagaatct tcatttatgg
     9481 tcacctacat ttactagtgt tcttccttgg tgtgttccag caagtagttt agatgctgtt
     9541 tcaggcaatt gatccagagt aatctcttgg acgaccagtt catctgcaat ctgcaaatcc
     9601 gccatctgtt gccataaaga aagacgttga tctattggta cattgacaga atcaattcca
     9661 atcaactgga tgtttcgcag aataaaaggc aagacagtcg cgtttagttt gattccccca
     9721 gcattgccac ataaagctac tgcaccgtta taagagatca atggaagaag actgcttagc
     9781 tgctcgccac ctactgtgtc gatgacatag tcgatttgct gttttcctaa agcttttgtt
     9841 gtctctggca gaaattcatc aggtcgtacg acttgcaaag cacctagact ttttagccat
     9901 gtgacagact cttcttttct ggaaaaggca atgattgact ggaatcctag tttatgtaac
     9961 agggctatcg ctgtactgcc tacaccgcca gaagcaccag taacgacaac ttttttatcg
    10021 gaggtcatac cttgacggat taaagcgttc acacacaaca tagcagtaaa ccctgctgta
    10081 ccaaggatca tcgcttgacg cagcgtcata ttttttggta gaggaactaa ccagtctcct
    10141 ggtacttttt gatattggct ataaccgcct ggatgggtga cgccaagccc aaaaccagta
    10201 gcaagtacaa gatcttcttt tgaaaaacgt ggattgttac tttggacgac agttccggat
    10261 aaatcaatcc ctggagtcat cggatatgtt cgtatcacct gaccagactc gctcattgcc
    10321 aatgcatctt tataattgac atcagaatag gcaattttta ccaagacttc attttcagcc
    10381 aactcagtaa atggcagatc actgattgct gtatggaaaa caggttcctt tattacctga
    10441 aaagatcgaa aagcgttcat tcgtgagaca ctccttttaa aattttcttt tatcatacca
    10501 taaagcctac tcttttattc ttgttcttcc gtaaatttag acaaaaatta ggtgaaaaat
    10561 aaagaatcaa acaactagaa tttattccaa aagataaaga aatattcagc aaaaaaaatt
    10621 ttacaaaggg ttgccttttt caataaattt cgtatactaa tacgagtggt agtaaacagg
    10681 aagtttacta ttagaaaact tcgattcgga ggaaattatg gaaattggtg agaagttaag
    10741 aaatttgcgc atccaaaaga atttgacaca agaagaacta ggcgaacgca cagatttgtc
    10801 aaaagggtat atttcccagt tggaacgaga tttaagttca ccatcaatgg aaacattctt
    10861 ttctattctt gaagtcttag gtgtgactcc agaagaattt tttcatcaag aagcagcgaa
    10921 tctgcaagtg gtttattcaa aagaagatca tacggtttat ttcgatgaag aaaacgggta
    10981 tgaattggaa tggctcgtgg ctgattcgaa tgaaaaagaa atggagcctg tcttgctgac
    11041 ttttgaaggt gcaggagaat ataagacgtt tgaaccttca ttatctgaaa cgtttatttt
    11101 tgtattagaa ggggaattag agttaagctt aggagagacg gtctatgtgg caaaagcagg
    11161 acagtcgatc tattatcaag cgacaaaaca acatcaatta aggaatcatc tcaaaaaacg
    11221 aacaaaagta ttgattgttg caacgcaatc atatttgtaa tatagggggt aaaaacgtgg
    11281 ggaaaccaat catttcattt gatcaagtag tgaaacgcta tgatgatgaa acagtattaa
    11341 aaaaagtaag ttttgagatc gaacaaggaa aattttatac actgcttggt ccttccggct
    11401 gtggtaaaac aacgatccta cgtattatag ctggattcac cgaagctaca gaaggggaca
    11461 tctattttga agggaaacgg ttgaatgatt tgccagctaa taagcgacaa gtaaatacag
    11521 tcttccaaga ttatgcactt tttcctcata tgaatgtttt tgataacgtt gcttttggat
    11581 tgaagatcaa gaaaatgtcc aaatcagata tcgaaaagaa ggtaaaagat gctttgcgta
    11641 tggtccagct tcctggatac gaaaaacggg agattagtga aatgtctggt ggtcaaagac
    11701 agcgtgtagc gattgcacga gcaattgtaa atgaaccgaa agtattgcta ttagatgaac
    11761 cgttatcggc gcttgatttg aaactacgta cagatatgca gtatgaactg cgggatctgc
    11821 aacaacgatt aggaatcacg tttatttttg ttactcatga ccaagaagaa gcgttggcaa
    11881 tgagtgatga gatttttgtc atgaataaag ggaaaatcgt ccaaagtggt acacccgtag
    11941 atatttatga tgagccaatc aatcatttcg ttgctgattt tgttggggag agcaatatcg
    12001 tggacggtgt aatgatcgaa gataatctag ttgagtttgt tggtaagcaa tttgaatgtg
    12061 tcgatggcgg gatgcgtcta aatgaaccag ttgaagttgt tttacgccca gaggatttga
    12121 cgatcacgac accagataaa ggaaaactag tcgtaacggt cgatactcag ctgtttcgtg
    12181 gtgtccatta tgaaatcatc tgttatgatg aacagcaaaa tgaatggatg gtccattcaa
    12241 cgaaaaaagc taaagaagga agcaaagtag gattggcctt tgagccagaa gatatccatg
    12301 tcatgcgttt caacgagtct gaagaagaat tcgatgcacg tcttgacagc tacgaagaat
    12361 aggggggaca agaatgaaaa caatgcgcag gatctattcc gtcccttata ttttgtggct
    12421 cggtttattc gtgatcgcgc ctgtattaat gatcatttat caatcttttt ttgatatgaa
    12481 tgggcaattc acgctgaata attatctatc ttattttacg tcggggacat cgctttctat
    12541 gacgatcaat tctgtatggt atgcattttt gatcacgttt ttcacgctga tcatcagtta
    12601 cccgactgct tattttttaa caaaactgaa gcacaagcaa ttatggctga tgctgattat
    12661 tttgcctaca tgggtgaatc tgctgcttaa agcttacgca tttattggga ttttcagtgt
    12721 gcacggcagc gtgaatcatt tctttgattt tatcgggatc gggccgaaac agatattatt
    12781 cacagacttc agttttctgt tcgtggcaac gtatatcgag attccattta tgattatgcc
    12841 aattttcaat gcattggaag agatgaatcc ttctttgatc agtgctagta gagatttagg
    12901 cgcgaatagc tttgagacct tccgacgagt gatttttccg ttatcattaa atggtgtgaa
    12961 aagtggtgtc caagcagtat ttattccttc tctttcttta ttcatgctga ctcgtctgat
    13021 tggcggaaat cgggtgatca ctttaggaac ggctatcgaa cagcatttct tggttacaca
    13081 aaactggggg atgggttcta caatcggtgt gattttaatc attgctatgt tcatcgtgat
    13141 gctcctgaca ggcgagaaga aaaaaggagg tcgctcataa atgaaaaaat ttaaatggtc
    13201 gtatctttat ttagggatcg tctttttatt cttatatgca ccgatcttct atctgatttt
    13261 ttattccttc aatgaaggag gaacgatgaa cagctttaca ggatttactt gggaaaatta
    13321 tgaagctgtt tttgaagata cacgtttgat cattatcgta ttgaatacgt tcatgctggc
    13381 tttcttatct gcattgatcg ccacgatcat cgggacattt ggggctatgg gaatctatta
    13441 cacgaaaaaa cgacgtgcac ggacagcgtt gttgagcatg aataacatct tattagtctc
    13501 tcctgatgtt attatcggtg ctagtttctt gattttcttt acactactag gattcggatt
    13561 aggttttcag agtgtattgc tttcccatat tgctttcagc attccaatcg tcgtcttgat
    13621 ggtcttgcca aaattgcagg aaatgaacga ttcaatggtc gatgcggcta gagaccttgg
    13681 tgctaataat ttccaagtag tcaaaaatat cattttgcct tatctttctc caggaatcat
    13741 agctggatat tttatggcat ttacctattc tcttgatgat tttgcggtta catttttcgt
    13801 cacaggaaat ggctttacga cgctctcagt agagatctat tctcgtgcaa gacaaggaat
    13861 cagtttagaa atcaatgcgc taagtgcgtt agttttcttg ttctccatga tattggtcat
    13921 tggatactac tttattagta aagaaaaccg tccaaaacgg ctgaagaaaa acagaagagt
    13981 acgcgagggg gtggcacggc tgcgatgaaa aaattacaat ctctgttttt aggtattctc
    14041 atcattattg cggtgctgct gcttggtgtt cgtcaacttg aaaaaacaag tggcatgagt
    14101 ggagcaaagg tacttaccat ctataactgg ggagattata tcgatcctag tttgatcacg
    14161 aaatttgaaa aagaatatgg atacaaagtg aattatgaaa catttgattc taatgaagcg
    14221 atgttcacaa aaatccagca aggtgggaca aactacgaca tcgctatacc aagtgaatat
    14281 atgatccaaa aaatgatcaa agaaaaacta gtacttccat tagatcattc gaagatcaaa
    14341 ggattaaata atattgatcc acgattttta gacttggatt ttgatccaga taatcagtat
    14401 tcgattccat atttttgggg aactttaggg atcgtatata acgataaatt ctttgatgca
    14461 aatcaaatta agcattggga tgatttgtgg aagccggaat taaaagacag tttgatgctg
    14521 attgacggtg cacgagaagt tatggggctt tctttgaata gtttaggcta ttctttgaat
    14581 agtaagaata tggcacagct gacagaagca gcaaataaat tgaatcattt aacaccaaat
    14641 gtcaaagcaa tcgtggcaga tgagatcaaa atgtatatga tcaatgaaga agcctccgtg
    14701 gcagtgacat tttctggcga agctgccgat atgatgtggg aaaatgaaca tttacattat
    14761 gtgatcccac cagaaggatc caatctttgg tttgataata tcgttatgcc aaaaaccgtg
    14821 aaaaataaag aaggtgccta tgatttcatc aactttatgc tagaaccgga aaatgcggca
    14881 caaaatgcag agtatattgg ttattctaca ccaaatgaag aagcaaaagc gttattgcca
    14941 aaagacattt cgtcggacga acaattctat ccatctgatg acacgatctc ccatcttgaa
    15001 gtctacaaag atctaggatc gaaataccta ggcatctata atgacctatt cttagaattc
    15061 aagatgtata ggagataatg gcaaaactca gtaagaatgg ggacttatca acgttctaaa
    15121 gagctaaaaa tcgtcctttt tctatctgag ttttccaatc ttctaccaaa agcggatgaa
    15181 acgccgcttt ttttgtgtta ttttattttt tctaccacgt ttttctacca gagaaaagtg
    15241 gacaaaacca ttgtaaaata gggcccgaga cgggctagta aatctaagat tgaaaaagtt
    15301 acagaaggat aaagtaattg gaaactatgc aaatatactt aacc
//
LOCUS       DACESX010000060        15149 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.060, whole genome shotgun sequence.
ACCESSION   DACESX010000060 DACESX010000000
VERSION     DACESX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.060"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(108..488)
                     /gene="yajC"
                     /locus_tag="IXF88_001576"
     CDS             complement(108..488)
                     /gene="yajC"
                     /locus_tag="IXF88_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1508311.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(585..1730)
                     /gene="tgt"
                     /locus_tag="IXF88_001577"
     CDS             complement(585..1730)
                     /gene="tgt"
                     /locus_tag="IXF88_001577"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1508312.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1948..2646
                     /locus_tag="IXF88_001578"
     CDS             1948..2646
                     /locus_tag="IXF88_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1508313.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2814..5453)
                     /locus_tag="IXF88_001579"
     CDS             complement(2814..5453)
                     /locus_tag="IXF88_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1508314.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6072..7103)
                     /gene="queA"
                     /locus_tag="IXF88_001580"
     CDS             complement(6072..7103)
                     /gene="queA"
                     /locus_tag="IXF88_001580"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1508315.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7296..8471
                     /locus_tag="IXF88_001581"
     CDS             7296..8471
                     /locus_tag="IXF88_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1508316.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8475..9116
                     /locus_tag="IXF88_001582"
     CDS             8475..9116
                     /locus_tag="IXF88_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508317.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9113..10030
                     /locus_tag="IXF88_001583"
     CDS             9113..10030
                     /locus_tag="IXF88_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1508318.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10027..10698
                     /locus_tag="IXF88_001584"
     CDS             10027..10698
                     /locus_tag="IXF88_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508319.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11239..11727)
                     /locus_tag="IXF88_001585"
     CDS             complement(11239..11727)
                     /locus_tag="IXF88_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ1508320.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11928..12194)
                     /locus_tag="IXF88_001586"
     CDS             complement(11928..12194)
                     /locus_tag="IXF88_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1508321.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12194..12457)
                     /locus_tag="IXF88_001587"
     CDS             complement(12194..12457)
                     /locus_tag="IXF88_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1508322.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12450..12722)
                     /locus_tag="IXF88_001588"
     CDS             complement(12450..12722)
                     /locus_tag="IXF88_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508323.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12933..13118
                     /locus_tag="IXF88_001589"
     CDS             12933..13118
                     /locus_tag="IXF88_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1508324.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13260..15083)
                     /locus_tag="IXF88_001590"
     CDS             complement(13260..15083)
                     /locus_tag="IXF88_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1508325.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         4392 a         3246 c         2856 g         4655 t
ORIGIN      
        1 ttcaatattg agcaatatat cccatgctct tatctctttc ttttttgaat aaaaaatgag
       61 gtaggacatc tttctgccct acctcgtctt taaataagct ttgatactta tttttcttcc
      121 ttctcttctg tctcttccag cgtttctgtt gtttcagttt ttacttcaac tgtctcagct
      181 ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg ttacgacagt ccctggtttg
      241 accgttttga ttgctgcacg atcgtactca aggaagatcc cttcgcagtc taaaacaact
      301 gtccgtttgt cattgtcgat ttctgaaaca actccatgta atccgccgat cgttacgact
      361 gaatcacctg gtttcatgct atctaacaag ttttgacgtt cttgctgttg tttcttttgc
      421 gaacgtgaca taaaaaacca cattgctaat agcgcgataa acatcacaag cattggtaat
      481 gttcccattt ttttcacctc actaattctg tatctacaaa aaacacttta gcagaaaaat
      541 gctgataaca ctagtttttt acttaatttt taaaagattt tcctttagaa gctttttgcg
      601 ttttctttat tgaacccgta ttcttcaaaa aaggcttctc taaattcaag caaattatca
      661 tccatgattg cttgacgtac ttgtttcatt agattcaaca agaagtataa attgtggtaa
      721 gaagtcaaac gaatcccaaa tgtttcatca catttgatca aatggcggat ataggcacgt
      781 gtatagtttt tacatgtata acaatcacat ttctcatcaa ttggacggaa atcatgggca
      841 tatttcgcat ttttcactac tagacggcct tgagaagtca tacatgtgcc atttcgagcg
      901 attcgagtag gcaagacaca gtcaaacatg tctactccac ggataacacc atcgatcaat
      961 gaatcagcag caccgacacc catcaagtaa cgtggtttgt tttcggggat caacggtgta
     1021 gtgaattcta atacacgatt catttctgat ttaggttccc cgacagataa cccgccgatt
     1081 gagtaaccag ggaaatccat cccgactaga tctttcgcgc tttgacgacg aagatcttca
     1141 aaccctgctc cttggatgat tccaaataat ccttgacggt ctggattagc atgagctttc
     1201 aatcctcgtt ctgcccatcg agacgtacgt tctactgatt ttttcacgta gtcgtaactt
     1261 tcatcgaatg gtggacattc gtcaaaactc atcatgatat cagagcccaa tttattttgg
     1321 atgttgattg ctttttctgg tgataaaaac atacgcgaac cgttcaagtg atttttgaaa
     1381 tgcacgcctt cttcttcgat attccgcata tcacttaatg aaaaaacttg aaagccgcct
     1441 gaatctgtta aaatcggttg atcccagttc ataaatttat gcagtccgcc agcttcttct
     1501 actaagtctt caccagggcg tagccataaa tgatatgtat tacttaaaat aacgccagca
     1561 cccatttctt tcaactcttc tggggacatt gtttttactg tagctaatgt tcctactggc
     1621 atgaacattg gtgtggggaa agttccgtgt ggcgtgatca attcccctaa acgcgcgcca
     1681 gtatgttttt ctttcttgat taaacgatag cgaatggcag gttgcgtcat tcatttccct
     1741 actttctata taaaatatct tgacaactcc caccatcata atcgtttcaa gtgctttttg
     1801 caagttccct cggcttcctt ctactttatt taatattctt ttttttatcc tttttttatg
     1861 aataacaaaa aaaagaaaaa cattatgaca gaaatatttt ttgatacatg gtatattggt
     1921 gttattaaag aaaaggaggg aaaaatcatg caatcttctg cagaattaaa acgccaagca
     1981 aaagagagtc taaaaggacg ttggggacaa gctgttttac taaatctaat ccctacattg
     2041 attggtattg ctactattct tcttattatg attcctatta tcttgtttgc caccaccata
     2101 tatgatggat cagcgagcgt ctcagaagcg acaacttcag gaggcggtag ttcaggaggc
     2161 ggtggcggaa ttatttccag catcattagc gctttgttca tgagcggtat ttcttggact
     2221 tatcttgata ttttacgtgg acaaaaagat cagatcgaac cattcaaaga tgcatttcgt
     2281 ggattcaaag gtttgtttat cggcggagtg atcagcttag ctgtgctgat ctcgatcttt
     2341 acaacattat ggggtttgct attagttatt cctggaatca ttaaaagcta ttcttattct
     2401 caaagctatt ttatttacta cgatatcgtc caagaaacag gacaaacacc taaagtactg
     2461 gatacgatca ctgcaagtcg aaaattgatg gatggttata aaggacaact tttctggtta
     2521 gatctttcat ttatcggctg gcatatctta gcattagcaa ctgctggtat tggttatcta
     2581 tggctcagcc catacatttc tgcaacaaaa gcagcatttt atgaacaatt gccaaaagat
     2641 atttaaaaga gagtgtgact tgtgaccgag ggggtggcag gagaacaccg tttatcgaga
     2701 ggcagtaaaa gaaacattca gcccattagc aaaaaggagg tgtgacaaca ttcatgtcac
     2761 acctcctttt tgtatagttc cagtaaaaag aaaatcaact tagaaacagt taatcattcc
     2821 tcttcttctg cttccggtaa tcgatggaac ttgatcagtt cattcaccgc gattggtacg
     2881 atagataaaa tccctaccaa gaaccagtga ttcaaactga tgtgtactag gccgaatagt
     2941 tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag ctaatgccag taacgccatt
     3001 tctaccaaag ctttattggt cgctaaattg actttgaaga tcgattggct gctgcgtaca
     3061 ttgaacacat gcagaatcga tgaataagct aagatcaaga atgcaacagt ttgaccgaca
     3121 tgctgacttg cgtctaccca tgggctgata gtatcgacat aactacctaa ataataacca
     3181 aacaaggtta cgatggtgaa gacccctgca tttatggcaa ttttttgcaa taaacctcga
     3241 gcaaagatcc cttcattttt cggaatgggt ggctgatcca tgatgccgtg ttccgctggc
     3301 tctcttccta aagcaaatcc tggaagtcca tcagcaacta cattgacaaa cagtagttgc
     3361 acagctgtaa atggtgctcc ccatcctaac agcatcgcaa tcaaaacaat aaagatctcc
     3421 gaaatattac agcttagcaa aaagttgata gctttacgaa tattctgata aaccgaacgt
     3481 ccttgactga cagcgtctac gatcgtcgca aagttgtcat ccgtcaaaat catatctgcg
     3541 gcactttgcg ccacatctgt tcctgtgatc cccatggcac acccaacgtt acttgctttc
     3601 aacgctggtg catcattcac accatcccca gtcatcgcta cgactgcacc cgtacgttgc
     3661 caagatttga cgatccggat cttgtcttct ggcgttaccc gagcatagac agagagagct
     3721 ttgacacgcg catctaattc ttcgtctgat aattgatgca gttctgatcc tgaaagtgct
     3781 tctgacggat cattcaagat acctaattct ttcgctatgg cactggcagt caccacatga
     3841 tctccagtga tcatgactgt tttgatgcca gcctttttcg ctcgcttgat tgctccttta
     3901 ctttctggac gtggtggatc gatcattccg atcagaccaa tcaaacgtaa gtctttttcc
     3961 agtgcttcag aagaaatatc ttgcggttct tcgtcataag tagcataacc aacagcaatc
     4021 actctcagtg cccgtttacc aaagcgatca ttaacgatcg ccgcttgttc tacatcgcct
     4081 gaactaaagc gcggagcaag aacatcgaat gctcctttgg tgattgagat gtatttttcg
     4141 cccatttggt gaactgttgt catcattttt cgttcagaat caaaaggaag ctcgtttact
     4201 cgtggatatt tttcttctaa ttctgccttt gtatgataat tctcttcaac agcccttacg
     4261 atggctactt ctgttggatt acctttgata acaaggtcgt catcttcttg ctcgaccgtc
     4321 acatctgtac atagtgcagc cattttcaat atttccatcg cgctgttcgt cattgaatct
     4381 tcgatattcg ttacttcatc ttctttgctc catacccggc ggacgcgcat ttcattttgt
     4441 gtcagcgttc ctgttttgtc tgagcagata acattagcgg ttcctagcgt ttcaactgct
     4501 ggcaactgtc ggatgatcgc atgtttcctc gccatcttct gcacaccgta tgcaagtgtc
     4561 aacgtaacaa taacagtcaa ggtttccggt acagctgcaa cagccaagga aatcgaagtc
     4621 atgaacatct caaggagtgg ttctccttgc agttcaccaa tcacaaatac taaagccgca
     4681 gccgctaaag cgatcatact gattcgtttg cctagctgat tcaatcggac ctgtaaaggt
     4741 gttttttgca tagtattttc gttcaaaaga tccgcgatct tacccatttc tgtagccatg
     4801 ccaatggcag tgacgatcgc tttcccacga cctgcagcaa ccgtacatcc tttaaatacc
     4861 atattaagtt ggtcacctaa tgactcatct tcttcagcaa taaatgctgc atctttttca
     4921 actgcttcac tttctccagt caacgccgat tcttctacac gtaattgtga tgcttcgatc
     4981 aaacgggcat cggcagaaat agcatcaccg gactctaaaa caagaatatc acccactacc
     5041 agttgctctg actcaacagt ggtcactttg ccgtctcgta tgacagttgc cgtttgttta
     5101 ttcatatctt gcaaggattc cagtgctttt tctgcgtttc cttcttggac aatcgccaat
     5161 accgagttga tcacaacgat agcgatgatc agcagtcctt caaataaatc cccatgttct
     5221 gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa gaaggatgat cgttgtaaaa
     5281 tcggacaagc ttcgaaagaa ttttttgatc atcgattctt tcggcgcctc atcgaattta
     5341 ttcaatccct gcttttttcg ccgttcatcc acttcttgtg aagacaaccc agtatctgga
     5401 tctgcttctg ttaccttctt tacttcttct attgtctgct tgtattcttc catcctattc
     5461 ctcctttaaa atcttcgtac aaaaaaaggt acttatttta ctataacatc ctttttttat
     5521 ttacttcaaa taaagggctt atcgattatt tgttaagaaa gcgtaaaatt taaagtgctt
     5581 atatttgcaa acgctaccaa aggatgctat tatatagatg taaggaagga ggataccatc
     5641 aaatagacag aagaatttct cgatagaaca gtgtaagcaa acaaagttgt tagaagtaca
     5701 ggtttcaccc agacatgcgt gtagagtaca aacctataaa tagtgaatgc gtatggatgt
     5761 ttggatcacg aagtatccga aacaagaagt aatgcgatac gtaacaattg aataacgtaa
     5821 acgtctgaaa attgaatagg aacaagagat gtttcaaatg ttgaagatgt aacgaaaaca
     5881 aaatttttgt ttaacatgaa gaaattcaac accgtgcttt gcatgtatga tggaaagtta
     5941 caatcatttc tgtttatttg aatccttccg aggtacaaga aagccgagct tatcgagagc
     6001 tcggcttttt ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc
     6061 ttttcataac cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct
     6121 tcaatcgcat gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac
     6181 atgactaaag tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat
     6241 tcatagccag gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta
     6301 aatttcgttc caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc
     6361 ccgccatttt tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat
     6421 tcactatgca tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac
     6481 cctacatgta gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat
     6541 tctttcgtaa aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag
     6601 acagtttgat agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt
     6661 tctcccaatg attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt
     6721 ccgccgtgtt ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt
     6781 cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt
     6841 gtattcgtta gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa
     6901 cgagcaggca gaactcgtgt attattcata actagcgcat caccaggctg taactcttca
     6961 atgatttcat ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa
     7021 cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa
     7081 tcaaaatctt ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct
     7141 atcatgtttt tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc
     7201 taaataaaaa aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat
     7261 agttcttcat tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa
     7321 aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg
     7381 agttcatttg ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca
     7441 accgaatgac tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa
     7501 ttgatccagt ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc
     7561 ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag
     7621 aagaacggaa taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt
     7681 tagatcgcta cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg
     7741 cccttgcagc gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa
     7801 tcactcgtga ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga
     7861 tcgtatttgt aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca
     7921 tgagtgaagg ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa
     7981 acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga
     8041 caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac
     8101 aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt
     8161 cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat
     8221 ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc
     8281 gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac
     8341 agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga
     8401 tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta
     8461 aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag
     8521 gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac
     8581 cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca
     8641 gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg
     8701 taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg attttacgta
     8761 atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg
     8821 ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca
     8881 tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata
     8941 tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc
     9001 tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag
     9061 gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg
     9121 tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc
     9181 tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga
     9241 agcacagatc ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac
     9301 cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga
     9361 tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc
     9421 tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata
     9481 cggtcaaacg tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa
     9541 agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga
     9601 attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt
     9661 ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta
     9721 tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat
     9781 cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc
     9841 tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa
     9901 taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa
     9961 taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga
    10021 agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag
    10081 gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt
    10141 gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa
    10201 ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg
    10261 atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt
    10321 ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa
    10381 aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta
    10441 gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc
    10501 atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc
    10561 cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca
    10621 ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc
    10681 agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt
    10741 cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc
    10801 cttatgggca aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat
    10861 actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca
    10921 ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga tctctaaata
    10981 ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat
    11041 ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg
    11101 tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat
    11161 ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct
    11221 aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg
    11281 atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa
    11341 gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat
    11401 ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt
    11461 cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc
    11521 ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt
    11581 gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt
    11641 cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc
    11701 tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca
    11761 acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta
    11821 aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt
    11881 tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt
    11941 gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga
    12001 tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa
    12061 gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc
    12121 tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact
    12181 gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt
    12241 cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt
    12301 cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac
    12361 taataatctc gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt
    12421 tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata
    12481 taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc
    12541 aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca
    12601 cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac
    12661 gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac
    12721 acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca
    12781 cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca
    12841 aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt
    12901 agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat
    12961 ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg
    13021 taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt
    13081 atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc
    13141 atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa
    13201 acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt
    13261 tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc
    13321 aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca
    13381 aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata
    13441 tctacataac ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa
    13501 cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct
    13561 tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac
    13621 gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa
    13681 tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca
    13741 tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc
    13801 cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata
    13861 ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct
    13921 gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct
    13981 gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca
    14041 ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt
    14101 gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa
    14161 atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta
    14221 ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata
    14281 aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg
    14341 gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca
    14401 gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc
    14461 acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat
    14521 aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg
    14581 aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc
    14641 acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca
    14701 cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa
    14761 atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga
    14821 ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca
    14881 aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca
    14941 acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa
    15001 gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact
    15061 aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt
    15121 ttatagtcga aaatttatag taaacaaga
//
LOCUS       DACESX010000061        14750 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.061, whole genome shotgun sequence.
ACCESSION   DACESX010000061 DACESX010000000
VERSION     DACESX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.061"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            7..1746
                     /gene="yfcC"
                     /locus_tag="IXF88_001591"
     CDS             7..1746
                     /gene="yfcC"
                     /locus_tag="IXF88_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1508326.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1903..3087
                     /gene="iadA"
                     /locus_tag="IXF88_001592"
     CDS             1903..3087
                     /gene="iadA"
                     /locus_tag="IXF88_001592"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1508327.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3198..3401
                     /locus_tag="IXF88_001593"
     CDS             3198..3401
                     /locus_tag="IXF88_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1508328.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3502..4164)
                     /locus_tag="IXF88_001594"
     CDS             complement(3502..4164)
                     /locus_tag="IXF88_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508329.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4177..5679)
                     /locus_tag="IXF88_001595"
     CDS             complement(4177..5679)
                     /locus_tag="IXF88_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508330.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     FLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6227..7906
                     /locus_tag="IXF88_001596"
     CDS             6227..7906
                     /locus_tag="IXF88_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1508331.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8033..8512
                     /locus_tag="IXF88_001597"
     CDS             8033..8512
                     /locus_tag="IXF88_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ1508332.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8731..9411
                     /locus_tag="IXF88_001598"
     CDS             8731..9411
                     /locus_tag="IXF88_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1508333.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9419..10750
                     /locus_tag="IXF88_001599"
     CDS             9419..10750
                     /locus_tag="IXF88_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1508334.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10765..11727
                     /locus_tag="IXF88_001600"
     CDS             10765..11727
                     /locus_tag="IXF88_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1508335.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11838..12176
                     /locus_tag="IXF88_001601"
     CDS             11838..12176
                     /locus_tag="IXF88_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1508336.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12255..12962
                     /locus_tag="IXF88_001602"
     CDS             12255..12962
                     /locus_tag="IXF88_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1508337.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13065..14546)
                     /locus_tag="IXF88_001603"
     CDS             complement(13065..14546)
                     /locus_tag="IXF88_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1508338.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4663 a         2543 c         3169 g         4375 t
ORIGIN      
        1 ataaatttga agaaaacaaa ttcttcagga aaaccgaaac gcgacttcac taaattatcg
       61 acaccccata cgtatgtcat tatttttggt gtagtcatct ttgcatggat actgactttt
      121 gtcgttccag caggaaagtt cagcacgcaa gatattgaat acaaagatgc aaatggcgaa
      181 acaagtactc gtactgtttt acgtcaagat tccttccgtt atgcttacga gctggataag
      241 tcgtatgtgt ttgatcaatt agaagaactg caagatcatc cagcagaacg tgaaaaactg
      301 gatgttccgg aaaaaggatt ggaaaaagtc attgctgacg gagaaaaaaa tcttacgcag
      361 gaaaaactag atgaaatctc cttgacagat gatgtgcttt acgatcagta cggcgaaaat
      421 atctatgata cttctaaaaa attgcataaa acagccaaaa tatggggaac agatgatttt
      481 ggcggctttg gtttcttaaa ttttgttttt gaaggactag tatctggaga taagtatgga
      541 tctgctgtag ggatcgctgc attgatcctt gttgttggag gtgcttttgg tattattatg
      601 cgtaccggag cgatcgacgc aggtatctat gcgtttatca gtaaaactaa gggactggag
      661 cgtttagctt taccgctatt attctttgct ttttcattcg gtggcgctac attcgggatg
      721 gcagaagagg taattccatt ctcaatggtg atggttccat ttgtcattgc gttaggatat
      781 gactcgatcg tagcagtaac cgtgacttat gtcgcatctc aagtcggaaa tgcgacctct
      841 tggatgagcc cgtttagcgt agcggtggct caagggattg ctggaatccc cgtgttgtct
      901 ggtgcgacat tccgattgat tatgtgggta gttgttactg cattagccgc tggttatatg
      961 atgatatatg cagaaaaaat ccgtaaaaaa ccagaaagtt cgttgactta caaatcagat
     1021 gattatttcc gttctcatat caaaaagaca tctgatgaaa ataaaaaatt catgcttggg
     1081 cataaattga tcttattaga gatgttagtt gtccttgtct ggatcgtttg gggcgtgact
     1141 caaaaaggct attacattcc agaaatcgct tctcaattct ttgtgatggg attagttgct
     1201 ggaatcattg ctgttctttt caaattagat ggaatgaaag tcaatgacat cgcttcttct
     1261 ttccaatctg gtgcagctga tttagctggt acagcaatcg tagtaggtat ggccaaaggg
     1321 attcttcttg tcttgggagg atctgatgca tctgttcctt cagctttgaa tacgattttg
     1381 catgggatcg ggacggcgtt gaccggtgtt ccagctgtca ttggtgcttg ggcgatgtac
     1441 atcttccaaa gtttgttcaa cttggttgtt acatcaaatt caggacaagc agctttgact
     1501 atgccaatca tggctccact ggcagattta gtaggtgtat ccagacaagt agcagttctt
     1561 gcttatcagc ttggttcagg ttttatggac gcttttacac ccgtatcagc cagcttgatt
     1621 ggtgtgctag gtgttgcacg gatcgaatgg gcaaaatggg caagattcca aatcaagatg
     1681 caaggattcc tgtttgtttt aggaactatt ttcatcatga ttgccattgc aataggtctg
     1741 cagtagtact ggagacctgt aaataaaaaa gtaatccaaa cagtagataa ggactccaag
     1801 aaccgtcatt ttacggtaaa atcaaaggag taaagatcct atcgtttgga ttatttttta
     1861 agatcctatc atcagttata cttaaagaaa gaggaaaaat gtatgaaaat cattcgacaa
     1921 attgaagtgt ttgcaccaga ttacttagga aaaatggatg tactgattgc cggtgggaag
     1981 attttggctg tagaagaaca gctagaaggc ggctacgaag gagtggaagt agaagaactt
     2041 tcaggtgagg gaaaagtact aaccccgggt ttcattgact gtcatttcca tattttaggt
     2101 ggaggaggag aaggcggtta tcaaaatcga acgccagaag tcacattgag ccaattgact
     2161 acagctggtg taacgacagt tgtcggttgt ttgggaacag acggagaagg tagagatatg
     2221 actgcgttga tcagcaaagc gaaaggtctg gaagcagaag gaatctccac ttatgtatac
     2281 gaaggttcgt accgattgcc ggtaaaaaca gtaacagatt ctatcatcaa agactttttg
     2341 acaattgata aaattatcgg aatcggagaa atcgcagtat ccgatcaccg ttcttctcag
     2401 ccaagttttg aagagttcgc tcgtgcagta gcagatgctc gtgtaggagg catgttatcc
     2461 ggaaaagctg ggatcatcaa tgtccactta ggcggaggaa aaagaaagct agagctattg
     2521 acacgaatcg tagaagaaac agaaattccg atcacacaat ttttgcctac tcatgcaaat
     2581 cgcacaccag aattatttga agcttgcgtg gcatttgcca aacgtggtgg cacgattgac
     2641 ttcactgcta gcgaagatcc tgatttttgg gagaagacag atggtgaagt tcgcttcagc
     2701 aaagcactga aacgattgat cgaggaagat gtttctttag ataatttcac gatgacctca
     2761 gatggacaag gaagcttgcc ttattttgat gaaaacaatc atttcctagg gttaggagta
     2821 ggaagtgcca aggcattgct agtagggatc aaagaagctg tgcaaaaaga aagtatccct
     2881 ttggaaatcg ccttacgagc tataaccagt aatccagcac gtatattgaa attggataaa
     2941 aaaggaaaaa tcgaaattgg tgctgatgca gatctgtgta tcttggataa agaaacgctt
     3001 gatattgata cagttatcgc taaaggagaa ataatggtcc aagaaaaaga agtcaaagta
     3061 tgggggactt tcgaaaaaag tttttaatca acagctctaa ctatcttttt tagaaaacga
     3121 gcgttctgtt tgtctgtatc tgttgttgat gttaccctga aggtagatca ataaacaaac
     3181 taagaggagg aagcacaatg gcagatttaa aaggacgttc taaagatatg aaagataaag
     3241 tagtcggtga aggaaaagaa gcatacggca aagcaacaga cgacaagagc aaagaaacag
     3301 aagggaaagc acaatcacta ggtgctgaca tcaaagaaaa agctcgaaat ttaggcgacg
     3361 aagtcaaaga caagttttct cgaaaagacg aaaaaaaata aaaaccactt attactaatt
     3421 gaatcaagca aataaaaaat ccgaaccata tctgcgagac agatatggtt cggatttttt
     3481 atttgatcat tatcctgttt tttaaacttc gattttttga aattttttat tttttagttc
     3541 aaagacttca tcacagcttt ttgctacttc aatctcatga gtcacgatga taatacagcg
     3601 attttgttca tgggcgattt cttggaaaag tttgacgata tcttgtgaag ttgtttcatc
     3661 tagatttcca gtaggctcat ccgctacgat tagttgatgt tcacagcaga tagcacgaac
     3721 gatagcgact cgctgctgct gacctcccga aagtttggtc acctgttttt ttgctaatat
     3781 ttcatcaatt cctacacgag ccagtcgatc taaagcaact tgtcgataat tctcttgctt
     3841 ggtcttagtg atttccattg ctgctatgac gttgtctaga gccgataaat aaggaagcaa
     3901 gttgtaagct tggaaaataa tcgagacatc atttttacga taacttctta acccgatttt
     3961 gttcagtgaa cgatcttgat agtatacctt tccttctttt ggtgcatcta gtccagaaat
     4021 aagcgaaaga aaagtcgttt ttccagaacc acttgctcct aaaatagcgt acatttttcc
     4081 agcttcgaaa ttcagttcta catctttata aagcgcatct tgcggatctt gataccaaaa
     4141 tcccaattgt tctgtccgta acatcaaatt cctcctttag tttaatagga tttttcttgg
     4201 gtttaatctt aatatcccta tagaagctaa aaagacagaa aacagactga ttccaaatgc
     4261 tactccggct aatagtccca attgtgcagg tttgacattg atttccaatt cgctgacttg
     4321 atcagaaaca gcaaatggat tattcgtggt tccttgttga ccatcgcgtt gaccgccttg
     4381 ttcttgtctg ttacctggca tttgtccacc gccaggagct tgctggtttt gtgtcgtggt
     4441 ttctgtctgt tggtcaagta gctgctgtcc cacggcattc gcgacgacgt ttccgctaag
     4501 cgaagcaact cctaatgcaa gaatcataca aatagccact tcggtaaaca attggagaat
     4561 gattttactt ctggattctc ctaaagatag caagacgcct aactcatgcc gacgctcccg
     4621 aatggtaatc ataatgatca gagtaagtat gataataccg gctacagcca ctaggaaaac
     4681 aacattttta gaaaaacttg ccacgttgtt taaaggttcc aacattgact gatacatgga
     4741 atcatttgat tgtaagctaa atgtatctgt gtcgatcagc ttctcagctt ctttgagaaa
     4801 ctcatccatt ttatctggat cagatagagt gtaaatagca gaatctatcg tgttatcgtc
     4861 agatgtccca tttagttcat ttgcaaaagt gtaagaagtg taaagtgtat tcgacggatt
     4921 catgaagttg aatttcatac ccatactgcc gcttgtttcg ctggattcat aaattccttt
     4981 gatcgtcatt tcataagtgg tatcttcatc ttcagtactc gtgattgtga aagtatctcc
     5041 gactgccagg tcatttgttt ctgctagagt agaatcaatg actacattgt ttgtgccttt
     5101 gtcgctgtca tctattcctt caccatccgt gatttttgct gttccatcag aaaaattact
     5161 attttgtgca gttgctgaca caccactgat ttgaaagtct gcttgagtca tttgcggatt
     5221 cccgcccatt cctccagcag gttcaccgcc tgcatcagta gtttctgaac tgtcttcact
     5281 tgaatcagaa ctagtgatgg cagttattcc atcttttgcc atagctgagg cggaactttc
     5341 aaaggaataa ctctttacat tatctaattc agctattttt tccgcgtccg ttaaggaaac
     5401 tggtgtaaga gaaaaactgc ctggatctgg acgttcttca gtggtagaac cgcttgaaga
     5461 agtttgttcc tgttttttaa atgaagcttc tcgattggca cttaaagtca ctgttgctcc
     5521 aacttctttt tgtgcttgat tcgctgcttc ttctgcagcg cttcgaatcg tgaggccagc
     5581 tagcacaaaa ataagaatgg ctgtgaacac ggcaaacaat aacaatgaac gtccccattt
     5641 tacttttgta cttttccatg cacgtttcat aaaattcata ttttgcatcc ctcctagtag
     5701 atgcatttga taatagacaa cctatcttaa agctagctta aatcttatgc tgtttgcaca
     5761 ccatcaacat gaggttctgt atatataaaa agtcactata gttattgcaa aaaatgtggt
     5821 aaaatgaaaa aagtatgttt caaaccgacg aagaagaaat gaaagaggaa tagcaaaaga
     5881 aatcctagat gctacagagg gatttttacg tgcatagttg ttctaacaat tgctgatact
     5941 agcgatttag tttatttgtt gtataataat ggggaacagc tgaatactta tataagtatt
     6001 ctggctatag cgtttcgcaa aagatccatt ttgttaatga agtatatcca aaataaaatg
     6061 cccatccaac tgtcagaacg cggatgtgct ggtaattttc ctgctgattc tccataagtt
     6121 gagaaatagt gattttgcat atgcttcatt ggaatggctc atatatttag ataaagaaga
     6181 aaatcgacat ctttaaactt ggatgtcata agaagaggtg aagtttgtga gtaccattaa
     6241 aattatccca ttaggcggcg ttcgtgaaaa tggaaaaaac atgtatgtcg cagaagtggg
     6301 cgaggatatt tttattttag attgtgggct aaaatatcct gaaaatgagt tgttggggat
     6361 cgacgtcgta atccctgatt ttacgtattt agaagaaaac attgatcgtg tagctggtgt
     6421 ctttttaaca catggacatg cagatgcgat cggggcactt ccttacttgt tgtcaaaaat
     6481 ttctgtacca gtattcggga cagagttgac gatcgagtta gcaaaattga atgtcagtcg
     6541 taatgaagca gccaaaggat ttaaggattt ccatgtggta gatgagcata cagaaataga
     6601 ctttggtgaa acagtagtga gctttttccg taccactcat acgataccag attcagtggg
     6661 gatcaatctg aaaacaaaag aaggtagtat cgtttatacg ggagatttta aatttgacca
     6721 aacggctatc ccaatgtatc aaacagatta tgcacgattg gcggaaatcg gcaaagaagg
     6781 agtcttagcc ttactaagtg aatcggcaaa tgcagaaaat cctcagcctg tagcttctga
     6841 agcccaaata gcagatgaag tatttgatac gatcaaatat tgggaagggc gtatcatcgt
     6901 cgcttgtgta gcaagtaatt tgcaacgagt gcaacaagta ttggatgcag cagaaaaagc
     6961 acaacgaaaa gttgtcttga ctggtcaaga tttcgaacga attattcgca cagcgatgcg
     7021 tttagaaaaa cttcaattac caagtgaaga tctgcttgtt acgttgaaag acatgaaaaa
     7081 ctatgatcca gaagaactga tcattttaga aactggccga atgggtgaac ccatcaagtc
     7141 tctgcaaaaa atggcaaacg gtacacaccg taatgtcaag attgaagaag gagatttggt
     7201 ttatatcact actacgccaa gcatcgcgat ggaaaccatc gtggcgaaaa cggaagatat
     7261 catttatcga gctggtggaa cagtgaagtt gatctctgag aatatgcgcg tttctggcca
     7321 tgccaatcct aatgacttgc aattaatgat caatttgatg aaaccaacat attttgtgcc
     7381 agttcaagga gagtatcgtg aattagctgc tcatgcagat ttggctcatg cagtagggat
     7441 gccatacaaa aatatctata ttactggacg cggagacgtg ctggaatata agaacaagcg
     7501 tatgagtgtt gcaggaacta caagtgcaga aaatgtaatg attgatggat taggcatagg
     7561 tgatatcgga aacattgttt tacgtgatcg aaaaatcttg tcagaagacg ggattttcgt
     7621 agcggttgtc actatcagtc ggcgagaaaa gaaaatcatt tcaaaacctc aaattacttc
     7681 acggggattc gtttacgtaa aagctagccg tgatctgatg aaagaaagca gcaatatgat
     7741 tgaaacaatc gttgagaaac atttagaaag caatgatttt gagtggagca aattaaagca
     7801 agacatccga gatcaattag gccgttactt atttgaacaa acaaaacgcc gcccagtgat
     7861 cttgccagtc attatggaag ctactcaacg tcgaaataaa aaataaacaa aaagagtaag
     7921 atgaacctct ctatagagtg ctcgtcttgc tctttttttg cgaaaaacaa ataatttttg
     7981 aatacagcat ccctcgcttt ataataaacg atagaataaa gggagtgaat caatgatgag
     8041 tcaaacaaaa gtcgaacgtt atttagaaga acaaaagatc agctatcaac cacttgattt
     8101 tagtcatgtg gtagaagaat ctttcacaga agaactacaa aaaagaggga ttgatccagc
     8161 tttgatatgt aaaactctcg tagtgaaagg aaacaaaact ggggtgatca tcgcattgat
     8221 tcctttgaac gatcacttag attacaaaaa aacgagacaa gtgacgaaag atcgtaaagt
     8281 tggttttcct ggaatggatt ttgtattaat gcatacagga tatccccacg gagcaaacac
     8341 ccctattgga ataaaactcg cacatcctga ttattgtttt ttagcagaca gttctttaaa
     8401 agaaaaagaa gaagtaattg tgtcaagcgg tgaaatcggg agaagccttc ggataaaagt
     8461 agccgattta gaaaagatcg tccatcctat ttacgctgat ttaattagtt aagggacgag
     8521 aagagaagct aagcagagca gagacctcaa acttgagatt atatctgctt ggtacttggt
     8581 gcatcctgta ttatctgtta caattgcttt gtacatactg tactatcaat ccaatgaaaa
     8641 tatatttagc agctatattt tttcattgat gtgcaagtga tgaaatcgtt tttaataaaa
     8701 aagatacaga gaaaacgcga ggtgtaaaat atgaaaatta cgtatcatgg acattcctgt
     8761 ttaacaattg aaatggaaaa tggccaacgc gtgatgattg acccgtttat tacaggaaat
     8821 ccattgacag acttaactgc agaagaagta aagacagatt ggttgctgat tactcatggt
     8881 catagtgacc acataggaga tatgattcct attgcaaaag aaaatcaagc cacaattatc
     8941 agtattgttg agattgctga ttatgcgaac agcagaggag tagattcatt cggtatgaat
     9001 atcggtggaa aacatgcgtt tccattcggc gtggtaaaat tcgtacatgc acagcatagt
     9061 tcaagctatg aagtagacgg tatcgttcag tatatgggcg agcctagcgg aattattatt
     9121 caagcagaag gaaaaacgat ttatcatgca ggggatactg cttatttcag tgatctagga
     9181 ttgcttgcag aagaattcga tattgatgtc gctttcttgc cgattggcga taactatacg
     9241 atggggccag aagatgcagc aaaggctgcg caaacaattc gtgccaagaa agtagttcca
     9301 atccattaca atactttccc tgtcattgaa caagatcccg aatcttttgt agagttactg
     9361 gaagatagtg aaggaaaaat aatgagcgtc tcggaaacgc tggaagtgta ggcagaaaat
     9421 ggcgacaaaa catgatttga ttttagaata tattgaaagt ttacctgttg gcaatcgaat
     9481 ttctgtaaga agcatcgcaa aagaattgaa tgtcagtgaa ggaacagcgt accgtgcaat
     9541 caaagatgca gaaaatgtcg gactagtttc aacaattcaa cgagtaggga ccatccgtat
     9601 cgaacgcaaa ttaaaaaaac atattgaacg tttgacattt ggcgaggtcg tcaggatcat
     9661 tgaaggggac gttttaggcg gggcggcagg attagacaag gtcttgaata aattcgtcat
     9721 cggcgcgatg actaaacatg ccatggaacg ctatattacg ccaggatcac taatgatcgt
     9781 cggcaaccgg gcggaagtcc aaaaattagc attggaagat ggcgccgcag tattgatcac
     9841 aggaggattc aatacgactg ctgagattgc aaagctagct gatgatcttg aaatgccgat
     9901 cttacgcacc tcttatgata catttacagt cgcaaccatg atcaaccgag cgttaagcga
     9961 ccaattgatc aaaaaagata tcatgcttgt cagtgatatc tatatgccag ctgaaaagac
    10021 tcattatttg cagcagacag atacgatttc tgattatcag cgtttgtcag aagaaacgca
    10081 gcattcgcgt tttccagtcg tcaaccgcca tcatcgtttg atggggattg ttacggcaaa
    10141 agatgtttta gggaaatcgc caaaccagat catcgatcgg gtgatgacga aagaaccaat
    10201 cagtgtaaag aaaacaatga gtattgcctc tgttagccat caaatgatct gggatgggct
    10261 agaagtcatg ccggtcgttt ccgatgactt gacattggaa ggcttgatta cccggcaaga
    10321 tgtaatgaaa gcgatgcagc ttgtccagcg acaaccgcag attgcagata cgatttcgga
    10381 tcagatatct ggaaatatcc atacgattga tactgaccgt gaaggaaatc gtttggaaaa
    10441 ccctaagttc aaattcgtag tagcaccaca aatggtcaat ggcgttggaa ccatttcttt
    10501 tggcgtactt agcgaaatca tttctgatgt cgcacaaaaa acaatggtga tgaaccaaaa
    10561 acgaaatatc ttgattgaac aagtgaacct acattatttg cggctgatcc aattggaaag
    10621 cgaattagac attcgtccac gtgtattgga aatcggcaga cgttccgcta aactggatat
    10681 cgaagtgttt attgaaaaca ccatcgtttc caaagcaatc gttgtttgtc aagtaatgga
    10741 acgttcttag aaagcaggag tgaaatgaca gtacagcaag aaatagtaga tacaataaaa
    10801 aaatacgata aaatcattat ccacaggcat ttgcgccctg atcctgatgc attaggatca
    10861 caagtaggat tagccgaact attacgggaa agtttcccag aaaaagaaat acgtcaagta
    10921 ggtgaaacga ttgaagggct tcgcttttta gcagaaatgc aagaagttga agatgtcttc
    10981 taccaaggag cgctagtcat cgtgactgat acagctaatt cacccagaat cagtgatcaa
    11041 agatatcagt tggcagaaaa actgatcaaa atcgaccatc atcccaatga tgacccttat
    11101 ggagatcttg tgtgggtaaa tacaaaagca agtagctgta gtgagatgat tgcagaattt
    11161 gctttgatgt ttcctgaatc tttaaagata aacgttaagg ctgcacggtt actttatgca
    11221 ggaatcgtcg gagatacagg gcgctttttg tatccctcca cgacgtcccg tacattagag
    11281 attgcttcga tattgagaaa ttatccattt gatgcaagtg ctttgaacag agagattgaa
    11341 caaatgccga tgaaagtcgc taaactttcc ggttatcttt atcaaaatat ccaggtggat
    11401 gaaaatggtg caggaagaat aatccttccg caagaaattc ttgataaata tggaatcgat
    11461 gattcagaga cagcttcgat cgtttcactt cctggagtga ttgacgaagt gctggcttgg
    11521 gggatttttg ttcagcagcc ttcagggtat taccgcgtac gtttacgttc taaggggccg
    11581 atcatcaatg aattggctaa acgccatcat ggaggaggcc atccattagc aagtggtgcg
    11641 aatgcaaaag atttagcaga agtagaggaa atctataaag agatccaaga tatatgccaa
    11701 tcgtataaga gcagaaaaac agaatgatac ttcgttcgaa aacgtgcctt ttgtagtaaa
    11761 aatcacaaat gtatcttttc aagatcttct ttttccgtta caatagtgct cactaaagat
    11821 tttaaaggag aaagaagatg acattaccaa attgcccaga atgtggctca gagtacacat
    11881 atgaagatcg aggcttgttg atttgcccag aatgcgggaa cgaatggaac aaagaagaac
    11941 aagaaactgt catagaagac ggattagtgg tattagatgc aaatggaaac cgtctgttag
    12001 agggcgatag cgtgacagtg attaaagact tgaaagtaaa aggtgcgtca ggtgccatta
    12061 aacaaggaac aaaagtcaag aacatccgtt tagtagaagg cgatcataat atcgattgca
    12121 aggtagaagg atttggaccg atgaaactga aatcggaatt tgtgaaaaag aattaaagaa
    12181 tattcaaggt cttttcacat tcttttccta ctactaatga taaaataaag taaacgaaaa
    12241 ggagtgaaac gaaaatgtat ggttatggaa tgtatggttt tttagatccg acatttcttt
    12301 tggtcattct tggattagtt atttccggaa ttgcgtcagc gtacgtcaat agtactttcc
    12361 gaaaatacga tcaagtgaga agcaaaaaca atgtgactgg tacacaagca gcacaatata
    12421 tattacaaag tcaacagata aataatgtcg gtgttcaaca aattgccgga gatctcacag
    12481 ataactacaa ttcaggtaat aagatgttga gtttgtctca agcaactgca cagtctactt
    12541 cagttgctgc gataggtgta gcagctcatg aatgcggaca tgcggtacaa gatgcttcgg
    12601 gttatgtccc attgaagatt cgtgcagcac ttgttccagc agctaacttt ggttcgatga
    12661 tttcgttccc cttgattatg gtaggtgtat tgcttagctg gaactctacg ttaatcaata
    12721 tcgggatttt cgctttttct ttagcattac ttttccaatt ggtgacatta ccagtcgaat
    12781 ttaacgcgtc tcggcgagca atccaaattt tgagtgaagg cggactactg acggaagaag
    12841 aagtaccgat ggcaagacac gtattatttg cagcagcact gacttatgtt gcagcagcgc
    12901 tttcgacatt cctgcagctg ttgcgcttga tcttattgtt cggcggcaac cgaagagatt
    12961 aaaataaaga agttgtgaaa tcagcgcttt gatctgagct aataccgaga gttagctgat
    13021 tgaataccat ttatttggaa taagaggatg tgacaagaat tttgtcacat cctctttgtt
    13081 ttgttctcat aacctttttt catatacagt tcatgaaccg cttttattaa cttgccaaat
    13141 aacactgtat tgtgcgttgt atccagatag aaagtctccg tttcatctga aattttctca
    13201 tgctgatctg taatgatcaa atcagcatgt tctgaatatg cggtaatttt aacagcatct
    13261 ttgcgaaata actgctgcaa atggctttga agatgataat ttcctgtaaa ctgtttggtt
    13321 atctgcaaat ggatgagtaa ctgctctttc gtttctgttt gtagtaagtg ttggatcaat
    13381 tgactaaggt attgactgat ttggtttctt tccccatttt ttgaaaaaat tgtatcgatt
    13441 ttttctctta atttcttttc ctgtataaaa gaactagaat gattggacgt ggcttctgga
    13501 aagcaaagtg tagaaaaact actgctggat tcacccaaca gttcgaaaaa aacaactgcc
    13561 aacacaagaa aataaaaata aattggtatt ttattttcta aatggttgag aggaaactgt
    13621 tgagttaagt aagtcatcaa ttgaccgact tgtcgacaaa taaggttttt gcttgtggaa
    13681 aataaaaaac caatctcctg tttttgtcga aaagagattt gatttggaaa aaaacaatag
    13741 aaaaaataat taaagtaggc tctctctgtt tctgtctctt cacaagggcg actcactgaa
    13801 aaagaagtaa agtattctga ataatcatgt gtatgctgca ataagtccat caattcagtg
    13861 atttcagaag aagtttttgg aaaatatgcc tgttgtttgt ttcgtaatat gaataaagtt
    13921 aacagcaagt agatattttt ttcttgtttt gaaatttttt ctatttgctc atctgtactg
    13981 ttcattttta gtgcatttaa ttcaagtgag gaaaatggcc aatcgttacc tccaaatgta
    14041 tctgaaagaa aaaagtaaag atagagacgg atagataact catttccttc aagagtacag
    14101 caatttttta cagataggat ccgtaaatgg aattttttta aggaatgatt gagctgtttt
    14161 gtcagttttt cgagatagga gggcgatata taaagttgtt caagtaaata tttttttggt
    14221 acagcagttt gtggataatt gattagtaac atcaaatagt taaaacgatt ggattctttt
    14281 aggtagcata attttaactt agaaaaaaca gctaactttt tagtttgatc ggtttctttg
    14341 gtttggatct ctcctttatg attagaataa ataaattcat caatcaataa aaattgcttg
    14401 agtgatacat ttatttttcg gatcagttct ttgattgtcc gttttggaat atgtaagtca
    14461 ttggataatt cttgtaaaga acaattattt ttttgaatga ccatttgtag aacaagtaac
    14521 tctttttttc ttgcttcaat tagcataaat acctcctttt tattctgttt ttgtatatag
    14581 ttagacaaaa actgtgagtg gaaagtcatt tcagtctgtg ttattctcac agagtgtcgc
    14641 cttctttttt atataacgtt atttaatttt tttgttacct tgtttattct attgtttatt
    14701 tcctagggta ttttgtcaag atattcttgt ttttaaggcg gaaaaattga
//
LOCUS       DACESX010000062        14699 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.062, whole genome shotgun sequence.
ACCESSION   DACESX010000062 DACESX010000000
VERSION     DACESX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.062"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            257..577
                     /locus_tag="IXF88_001604"
     CDS             257..577
                     /locus_tag="IXF88_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1508339.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            738..1643
                     /locus_tag="IXF88_001605"
     CDS             738..1643
                     /locus_tag="IXF88_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1508340.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1640..2413
                     /locus_tag="IXF88_001606"
     CDS             1640..2413
                     /locus_tag="IXF88_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1508341.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(2590..4014)
                     /locus_tag="IXF88_001607"
     CDS             complement(2590..4014)
                     /locus_tag="IXF88_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1508342.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4225..4671
                     /gene="nrdI"
                     /locus_tag="IXF88_001608"
     CDS             4225..4671
                     /gene="nrdI"
                     /locus_tag="IXF88_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1508343.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(4741..6723)
                     /locus_tag="IXF88_001609"
     CDS             complement(4741..6723)
                     /locus_tag="IXF88_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1508344.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6737..7633)
                     /gene="murQ"
                     /locus_tag="IXF88_001610"
     CDS             complement(6737..7633)
                     /gene="murQ"
                     /locus_tag="IXF88_001610"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1508345.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(7636..8697)
                     /locus_tag="IXF88_001611"
     CDS             complement(7636..8697)
                     /locus_tag="IXF88_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1508346.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8909..9436)
                     /locus_tag="IXF88_001612"
     CDS             complement(8909..9436)
                     /locus_tag="IXF88_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1508347.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(9503..11005)
                     /gene="lsa"
                     /locus_tag="IXF88_001613"
     CDS             complement(9503..11005)
                     /gene="lsa"
                     /locus_tag="IXF88_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1508348.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(11263..12384)
                     /locus_tag="IXF88_001614"
     CDS             complement(11263..12384)
                     /locus_tag="IXF88_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1508349.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            12510..13451
                     /locus_tag="IXF88_001615"
     CDS             12510..13451
                     /locus_tag="IXF88_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1508350.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13594..14490)
                     /locus_tag="IXF88_001616"
     CDS             complement(13594..14490)
                     /locus_tag="IXF88_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1508351.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
BASE COUNT         4272 a         3122 c         2628 g         4677 t
ORIGIN      
        1 taataaataa caactccttt ctatgaaatt gcaaagcatt catcttcttt tctgctaacg
       61 atatatccgt caatgcttgt tacactttac taaaaagtat gagattgccg atatctggat
      121 aggtgtgcta tactacagaa aaagatatac gatctgccac cgggtagtaa aaaagaggat
      181 gatcattcca ttaggtcttt gaagggatga ttctcctctt tttgtgtatt ttttatattg
      241 aaggaggaac gtatgtatgg catggatgga attaatcata gcaggaattt tggaagtatt
      301 ctggtcaacg atgatgaaat ggagtgatgg tttttctaaa atcaattatt ctttttacac
      361 agtgattgga atgattgcta gtttttactt tttgtcaaag gcaatcaaaa gtttgcctat
      421 gagtctctct tatcctattt ggacagggat tggcgcagtt ggttcggtca ttattggtgt
      481 ggtcttgttt catgataagc tgaatctttc cacttggttc tttgtcgggt tgctgctcat
      541 cagcatcatt ggcatcaaaa tcactagcgg tcactagata cttagacaag cagacaagga
      601 ctgatcagaa aaagcggggc tttttatttg atattcctct ttctatgaaa aaacaagaaa
      661 ataagactaa tgacctagcg acttctctac tcatcaggtg tatgcttgta ctatctatag
      721 aaaaggagtg aatatttttg aaaagtcttt cggtccaaga attatccaag aactttggtc
      781 acaaacaagc cctcagcgat gtcagtttta gcttatctcc tggcaaaatc gttggattag
      841 taggtcctaa tggcgctgga aaaacaacga tcatgaaagc tattttagga ctgatccatt
      901 actcagaagg aacgatccgt attggtgaaa atgagatttc tccctctagt cataaaggat
      961 tagaacaagt aggagcattg atcgaatatc cgggtatcta tccattctta acaggttatg
     1021 atcatctgaa gttattctct gaaacaaacg atgtatctgc tatcgacact atcgtgaaac
     1081 agctcaagat ggaaaaatac attcacaaaa aagcgaaatc ctactcgctg ggaatgaaac
     1141 aaaaattggg aattgctctt gccttactga accatccgtc tatcgttatt ttagatgagc
     1201 cgatgaacgg tttggatcca caagcaacaa gagatgtgcg aaaaatcatt accgacttgg
     1261 ctactcaggg cacctctttc ttgatctcca gtcacttact cagcgagctt gaaaaaatgg
     1321 tcgatgagct gatcctgatc aatcaaggaa aaatcatcaa acaatgtaca atggctgaat
     1381 tgaaccaaac agcgcaagat ttcatcgtta tctctacgac aaatgatcaa gctgctcgaa
     1441 cgatcttgac agacgctgga tatcccttgg aagatcctga taaaatcaag cttctaaaaa
     1501 aaaacgaaga gttattagga actgttatcc agttattgtc tgcagaaaat gtccatgtga
     1561 cagatgtcca gcatctgacg aatgatttgg aatcttccct attggaaatt ctggattcag
     1621 taaaggggga agaagaaaaa tgacgttgct tataaaacaa gaattattta aattgatcaa
     1681 gaaaaagtca actgctgttt tatctgttct attagttgct ttgctcatag gaacagcact
     1741 tctagccaaa aaatacacga cgatcattga tccagttgaa atgactgctc agcttttcag
     1801 tgccacatct tggattgtct ttatcatgat tgctgctgca agtacgatca tttcaatgga
     1861 atcacaatat ggtacattga aaaatctcct ttatcgaaag tattctcgag gagaaatatt
     1921 agtaagtaaa tggatcacat tagttattta ttctatttat ttataccttt tagctattat
     1981 tgtcaccgtg ctgatgaaac tgatcctttt cccttcgatt tcctttacgg agaagatttc
     2041 tactggtcag acactgatcc aaagcttatt cacttatact ttgggtagtt atatcggttt
     2101 atggctaatt ttaagtttag tgttgatgat tgcttgtttt atcaatagtt ccggtgcctc
     2161 catctcagca gggatcgtct tttattttgc ttcatcaatc atatctggta tcctcatcgc
     2221 actcattcaa aaatgggagt ggatcaaatg gaatccaatc agtatgctga atctgcaaaa
     2281 ccaaattggc aacgaagaga ttatgaagca attgactcat ctttctacta atcaaatgct
     2341 gtttggcaat ttagcttaca ttgctttgtt cttagcattg ggatacctcg tattcaagcg
     2401 aaaaaatgta tgatggttca ttaacaaaga agttgtgaca gaagtggcca gtttcaagaa
     2461 atttcggctt atttttagaa gaagctactt cttgaacacc atttattcaa aaaaagaagc
     2521 gatgacacga atgttgtcac cgcttctttt ttgaataaac cacaggcttc acagcctctc
     2581 tttatacttc tatacgtgaa gcatcccagt ggcaaataaa accattgcga ttactccgac
     2641 catcgtcaca ccgataagta aaatcttatc taagtggata tgttgtacag gttcgttttg
     2701 ttctttacgt gctttgaggt aaaaataaat acctggtaag aaaagaacaa gaacaagcat
     2761 cacttcatta aaggctacaa ctagcatggc ccatacttga aaagcacttg ctaaaaaccc
     2821 tactaaaaat tggacccact ctttccgctg ataagaaagc ttcatttggt acagccctaa
     2881 gaaaagccac gtgaagaaaa tcgctgctgt accaagtgta taagcaaagt tatatgcact
     2941 gtcagtgaat aataatgaaa gtaaaaagac attcgtacat aacgtcgtca atacgatagc
     3001 ataagttggt gccccatttt tgttgagttt tccccatgct ttaggcaaaa gtccgtcctc
     3061 agccatattc cccattgttt caatcggcaa gattgtccat gagagccaaa ccccaactaa
     3121 agaaatgatc aaaccgccgt tgatcacgac cgccccccat ttaccaacca tcatttcaaa
     3181 tacatatccc atagcaggtt gcgctttgat cgtttgcagc tgattcatcg tcattgtgcc
     3241 atatggaagc atggaaacca agacgtaaat aatgatcaaa ccgaatattc ctagaatcgt
     3301 tgctttccca acatccgatt gtttttttgc tcgtgaacct acgacagaag ctccttcaat
     3361 accaacgaat acccagagca tcaccatgat gcaactcaat acttgactag aaaacgggat
     3421 cggctgccca ttcgcattca atatgatatc gctagaaaag ttattaatga attgttggat
     3481 cgaaaaggtg ctgatagcta ctacaataaa taaaaataat ggcactaatt tacaaaatgt
     3541 gacgatagca ttgacaaagc tagcattttc aactccacga ttcactagcc atgcatatcc
     3601 ccataagaag atcgtactca tcacgatcga tgccacattt tgaccacctt cgaatacggg
     3661 gaagaaataa cctaatgcac tcatcaacaa tgttgcgaac gccacgtttc ctaaccagca
     3721 tgtgagccag taaccccaac cgctgataaa accagcgaaa cggccaaaac cttcttctgc
     3781 gtaactaaag atcccgcctt ttaaatctgg acgtttttta ttgatattat tgatcgataa
     3841 ggagagcatc aacattgctg ttcctacgag tacccagctg aagatagctg ggccaccagc
     3901 cgatccggag atgtccgtca taataccaaa tacgccacct ccaatcgctg aaccaatcac
     3961 taaggcagca agtggaatca aaccaattcc ttttttctgt tgctctgtat ccatgtaagc
     4021 atcctccaaa gtaaaaccgc ttttatcaaa aagtataaca aacctttttt atttttactt
     4081 aattaataaa aactttaata gaaatataat tctaccgact tttttctcat tatacaagca
     4141 aagcgcctag tccctttcca aattacacca ccaaaaaaac ttaaaaagat gctatactaa
     4201 agggaaagaa ttgggggatg atccatgaac attttatata tatcgatttc aggaaatacg
     4261 cgtgcttttg ccaaacatct agcagaatat gctgaaaaaa tgcatgcaga agatcctgtc
     4321 aatcctgaag ttactttaaa agaaatccat gaaaatagtg attttacgaa agaaacagaa
     4381 ccatttttta ctttcgttcc tacttattta gacggcggga atgggttaga caacggtgac
     4441 acagaaatct tgaccgaaac gatgcgcgaa tatctggaat acgaagacaa tcataaactt
     4501 tgccttggtg tagtcggtag cggtaataaa aacttcaaca atcaatattg tctgacagcc
     4561 aaacaatacg cacaaagatt cggctttcct ttcttggcag attatgaatt acggggaaca
     4621 ccatctgatg tgacacgcgt ttatcaaatt ttggcagaaa aaaataaata acacaaaaga
     4681 agaagctgaa aaataaattt cggcttcttc tttttatgtt ctctattgtt tctattctta
     4741 ttaagctttg acatccatca caggtgcttt tgctttcgct ttacctgttt tttccaagac
     4801 aaagtttttt acacgatcca tattggttac caagacgatc atggaggttt ctttgtctgc
     4861 tgctttgatt gcttctaagt cagcttctgc tacaagcgta tcaggtgtca cttgctgtcc
     4921 ttctgttact ttcacatcga atggcgtacc gcctaagtca actgtgttga tgcccatgtg
     4981 aagcaagatt tctaagccat ttggtgtcgt aatgccgata gcatgttttg ttgggaaaac
     5041 agaacttatt actccagtaa ccggggaata gatatgtccg ttttctggtt ctacaaagta
     5101 accttcaccc atcatttttt gtgcaaaaac tgggtcactt gctttatcta aagaaacaac
     5161 tgttccgtca gcaactgatg ataagatagt cacactttca gctgaagctt gaggtactgc
     5221 tttttcaggt acttcattta ctgtggctgt ttcggtcgct agttcttctt tttctaaacg
     5281 gtctttagga ataccgaaga agaatgtagc aacgaagcca ccagcgtaag cagccaataa
     5341 accaaggata tagcctaacc atttaccatc agagatcaat ggaatcaaag cggctcctga
     5401 aggtccaata gcgattgctc cgacatttcc gatcattccg atcactgccc caccgatacc
     5461 tccaccgata caagcagtaa taaatggacg tcctagaggt aatgttactc cgtaaatcaa
     5521 tggttcgccg attcctaaga ttccgactgg taaagcccct ttgatcattt ctgttaattc
     5581 tttgtctttt ttgcagcgga tccataatgc caatgctgca cccacttgtc ctgctccggc
     5641 catcgcaagg attggaagca ataaggtacg accagtttca tcaatcattt gtacatggat
     5701 tggcgtcaag atctgatgta gtccgaacat gaccattggc aagaataatg tacctagaac
     5761 gaagccggca aatgctcctc caacaccaag tacccagttg atcccaccga ccatgccgtc
     5821 agaaatgaac cctgctaaag gcatgatgaa gaaaatttcg atcagaccga tggcaatcag
     5881 ggaaagagtt ggcgtaacga tgatatctac cgcatcaggt actactttat gcagtttttt
     5941 ctcaacgatt gacaataacc aaactgcgaa gataacacct aagatcccgc cttgtccagt
     6001 tgcaagagct tcaccggtaa acaagttttt cagtggtgct tctggattca tccctgtcag
     6061 catcacaaca gccccgataa ctccaccaag cgttggcgtt gcaccaaaaa cttgtgcagc
     6121 attgatacct gtatagataa ctagatagct aaacatcccg tttttcaaga tgttcatcac
     6181 atcgatatat tgttgccatg ttcctgcgtc tagtgttcca gctgtgacta agtttgctaa
     6241 aactgcggca atccctgcca cgatcccagt gccgacaaat gcagggatca ttggtacaaa
     6301 gatattagag atgtctttta ataccgattt gattttagag ggtttttgtt tcgatttttg
     6361 tgccgctttc atttcttgtg cccgttcgtt tacacggtct tttccagatg cagaagaact
     6421 tgctgtagtt gcactagcag aaggaagttt ttctcctaat ttcacgcctg ctaagtcaac
     6481 catttcctgc gctactttgt tcactttccc aggtccgatg atgacttgga gctgttcatc
     6541 ctctactacg ccaagtactc ctggaatcgc ttttaattcc gagacattta caagatcatc
     6601 attgatgacc ttcatacgta cacgagtcat gcagtggaca atgctttcaa cattgcccat
     6661 accgcctgct ccttgataaa tttgttttgc cagttcttcc aaagtcaatt cgttttttgc
     6721 catggtcttt tccccctcat aaggttcctt ttacaaaacc gtttgcttca gctaattttg
     6781 cttgagctgc ctctttatca ctatttgtca aaatcatgac gatagccagt ttgacatttt
     6841 gatctgcttc ctcaaagtac ttttcagctg tttcgtagtc agcttctgtc gcttgcatga
     6901 tgattcgttt tgagcgttca attagttttt catttgttgg tttgacgtcg accatcaagt
     6961 tattgaatac tttaccgata ccgatcatac tgatagtaga aatcatattc aaaatcagtt
     7021 tttgtgctgt tcctgacttc aatcgagtag agcctgtcaa aaattctggt cctgcatcga
     7081 cttcgattgg gaaatccgca tgctcgctga tttctgcttg tttattgcaa gaaatcgttc
     7141 ctgtaacagc accaatcgat tttgcgtaat ctaatccacc aatcacatat ggtgtacgac
     7201 cgctagctgc gataccaata actatatcat tttttgttaa attcagcttg attagatctt
     7261 cttgccctaa ttcataagaa tcttcggctc cttcgacggc tacagtcatt gcttcttctc
     7321 caccagcaat taggcctatg accatttccg gttctacacc gaatgtcggt acacattcag
     7381 cagcatctaa tacaccaagt ctaccactcg ttccagctcc catataaatc aagcgaccac
     7441 cttttttaaa ggattcaatc gtgttggcga tcactggctc gatacttggc agttcttttt
     7501 caacagcagc ggccactttc tgatcttctt tgttcatgag ttggactgct tgagaaatac
     7561 tcatttcatc taatccaaaa gtttcttggt ttctgcgttc cgttgttagg ttctctaaat
     7621 tgattttgct catttttatc ttccttcttt tcttataaat tgatagttca taccagcttt
     7681 gatttggttg atcagcggta gttcttcttt gatgactttg gctactacat tcactttttc
     7741 atccgctggc aaatcatatt tagttaattg gatctcaccc atatagcgca aatacttttc
     7801 gttatctaaa gtaatagatc ctttcatacg cttagctgta tttcttactg ggattggcgg
     7861 aatctctcga aaacgcgcat ctgcgcttcg tatgacatct cttgcatcat cttgccgatt
     7921 cgtatgtttt cctaatatat attcgtaaaa aacttcatct acaggttccg tatggagcaa
     7981 gactgtctgt tctttttgga aagaagcaaa ttgctcctga acgtgttctg acagacctgc
     8041 atctccgata taaacaatat ccgtgttact tgctaataga tcaagagcac ctgccagagg
     8101 atggatccct cgatgttctt ccaaagttgg taatccttga tacagtggac ctctcaactc
     8161 tgtatctcct ggtacaaagc cttgtacaag gaatccttga gttttgagcc attgattttt
     8221 ttcctgatac cattctttgt ccaaccctgt ttcaagacgt ggataataat tatgccaagc
     8281 ttcaatctgt gagaaatcag ccgctgcttc tttcaactca tcgatatcat taggcgtgat
     8341 cgtgctggca ttcaaactga ttttcaattg atgtgaccag tcggcgatct gctgattaga
     8401 aatatgataa tccattctta accctgttac accaattgct ttcaagggtg ttagatcttc
     8461 taatgaaaag ccagctcgtt tcaatgcctc tcctgaaata tcgaccatca actctagttt
     8521 attctttttc gcttcgcttc ctagttcgat caatcgattt tgataagccg aaacatcatc
     8581 ttccggtata tgcatggaag tgaaaatacc gacaaagcca ttggatgcca tttggcggat
     8641 atactgtttc ttctcttctg acaaagaatc attcataaat attgaaaagc caaacatatt
     8701 atccctcttt ttctgtatcc gtcttcattt ctatgactag agtatatatc taaagaaaca
     8761 aaatttcaat acttttgtta aaataattaa attttatttc taaaaattgg atttataatg
     8821 ccaaaagaat tgacacagcc acaaaaaagg cactgctaag caaaagcagt gccttgaaac
     8881 attttattta aataacacaa aagtctaatt actcataaga aattaattct gtattgtcta
     8941 ccatgaatgt aaagaaccct tcattaggca ttggatgaaa ataagcattg atcactctta
     9001 tcaaggcatt atgacagact aacaagtaga cattttcttc gtcttccatg cattctttca
     9061 ataaaggaac gatccgtgcg tacacatcta agaccgactc tccatttggg aaacgtacag
     9121 caaacgctaa acgcgctttt tgaaagtttt catctttgct tggcagtccg tcataatccc
     9181 caaaatccat ttctaccaat cgctcatcag cggtcatcgg caaattgaga cgatcaacta
     9241 ctgcttgtgc tgtatctctt gctcgttgaa gaggggaatg gatgattttc gtgattggaa
     9301 caggcaaatc tactaccttt tctgctagtg cctgtgcctg catctgtcct ttttcagtca
     9361 gcggcacatc tgctcgcccg caaactaatc cttgtgcgtt ccaagttgtt tctccgtgtc
     9421 tcgttacata aagcatgtga ttctctcctt cgttgacaaa gttctcctta accatacctt
     9481 gttgtctttg agattacaag gtttagattt tctcaagaga aataatctca gtagatactt
     9541 tatctagaaa ggtttgatca tgttccacta gtaacatagc aggtttcacg ttcaaaatca
     9601 gttgttctaa ttgttcttga ttgaagacat ccaaataatt caatggttca tcccatatat
     9661 agagctcagc tggttgggat aaagatttag ccaattccac ttttttccgt tgtcccatac
     9721 tcatctgttc gattttatta tgaaaaacat ctctttccat cccaagcttt cggagattat
     9781 tcaaaaatgc ttgatagtct actcggtttt tcttcgcgaa ttccgccaat gtccctcgat
     9841 tgtcttcata attttggcgt acgtaactaa tggtcaaatg tttgggctgg gatttttctc
     9901 ctacaacctt gccgttgaat gtccccagaa gataatggat aatggatgac tttcccgcgc
     9961 cgttaggacc tgaaattgcc atccgctgat gaggttcgat cgtaaaatga actggctcga
    10021 ataacagatt ttcataccct aattgaagat tttctacgct taacagccgt ttatgatgag
    10081 acacttggct attcatcgtc aaagcatcga tatactcgat atcttttaat aatttttctt
    10141 tctcactgat ctgggtttcc atccgattca cgattgcttt agatcgtttc atcgtccgtg
    10201 cagcatcagc gcctactgct cctttattca ctcgcctaga ttccgtatcg atgaatccga
    10261 cttttttctt tgttttgtcc ccttctcggg aacgagacca ttctgctttt tcagctgctg
    10321 ttttctttag cctgctgact tcttttttca atttttcatt tttagctatt tcaaattcat
    10381 cacgaagttt tttctgttct tcatagatag agaaattccc ttgataaagt tccagttggc
    10441 tcttttcgat tgctaaaaca tggtccacta cttcatcgat aaatccccga tcatgactca
    10501 ccacgatgaa tccttgtttt ttctttttca agtaagccgc tacttgtttt ctgccagaga
    10561 tatccaaatg attcgttggt tcatcgatta acgggaaatg agtgtcatcc acaaataaga
    10621 gtgccagtag gacttttgtc ttctctcctc ctgatagtgt gctgaattct ctccataaga
    10681 tttcaggatc tgtttgcatc agttggagct ctctttcaat ttcccatatc tcaaaatcca
    10741 taatctcatt taacacataa taggtcaaac gctctttatc ttttgtttgc tgcgggaaat
    10801 agacaaacgc ttgctgatgg atcacttgcc cttgataagg gagtttgttt tgcaggatat
    10861 tcagtaaagt tgtctttcct cgaccgtttc gtccgataag tcctagtttc cattgtgtgt
    10921 caaagtttag attggcttgt tcaaataata atgtgccttg gctgtcatag ccgaatgtca
    10981 gattttttat ttcgatttta gacattattc catcacctcg taaaaaaaga ccagtcctat
    11041 tttcagaact ggctaaagag gatgcaaggg ttggggcaca caaaaagacc catacatcga
    11101 aagaaccaag ccaatcccga aaatctgcac actgctccct tgtctccaca aggtacagtc
    11161 tgaatgcagc tttcatcaat cgacttaatt tttcaatgca cggatccctc actttgtcct
    11221 atatttgaaa ttacgatagc ataaagaaat tgtttttgtc aactacttcg ggagaatgtt
    11281 gaaaaatctg ctgagtgtct gtcctaagat ttctgttttt tctaatgttt gcctgtgttc
    11341 cattgcttcc tctaaatcga ttggtcctaa ttgaatgctt aagacaatgg ggagaaaatc
    11401 aaagagtcct tccagctctc ggtctctact tccgcaaata gcgattattg gttttcgata
    11461 tttttttgca agcactgcta cgccatatgg aacttttccg tggattgttt gctgatcaat
    11521 ccgcccttct cctgtaatga tgacatccgc ttgttcgatc tgtttttcta atccagtgat
    11581 ttggctgacc aactcaaaac cagagaccat cttcgctcct aacaaaaaaa gggctccgcc
    11641 aataccgcct gctgctccgc ttcctgggat gtcatctaaa tccacctccc aaacatcttt
    11701 tatctgctga gcaatctttt cagcttgttt atccaagaaa gctaacgtcg tatccgttcc
    11761 tcctttttgt cttccaaatt gccaagcagc tccttgttga cctgtatatg ggttggtcac
    11821 gtctgcagcg atgatcactt ttactttaga aacaagaacg ttccttcgat ccaaatgaag
    11881 ttttctagta gataacaaag gatttccttc tgtgtaccct tctaaagaag cacccaaaga
    11941 ttggagtaat cctagaccac catcgctaca tccgcttcct cctaaagtga caatgatttg
    12001 ttccacttct tgaagcagtg tatctttgat aagctctccc aaggcataac tagaagcaag
    12061 tcggatggtt tgatccgttg gtttatcaag taaatctaat cctaatactt gtgcagattc
    12121 aatgactgcg gtattttttc cttgccagtt cgtcaataaa taactgattt ttcgctgacg
    12181 aaataagaga tccatcccat caatcgtacg ccaggttccc ccgagcgcgt aatagattgc
    12241 ttctgcagta ttttctccgc catctgcaat cggcacattg atttttcttc ctttacggtc
    12301 ttcccaacca tttagaactg cctgatttaa ttcaaatgaa gaagcacttc ctttaaatga
    12361 atcgattgcc gccaaaatat ccatctatat ccctcacttt cttcttaagt aagacacttt
    12421 ttttcacttt tttcaaacaa aacaatgcta ttttcttctg tttttctata tactaaaagg
    12481 gaactcattg aataaaggag agaaaaaaca tgaaaatcgc aatagctgga gccggagcaa
    12541 tgggcagccg aatcggatta atgttgcatc aaagcggaaa tgaggtcgtc ttgatcgatc
    12601 gctggcctgc tcacattgaa gccattcgta caaatggatt gattgctgac tttaatggca
    12661 aagaagtagt tgcaaaactg ccaatctatt caccagaaga aatcatcgaa agcaacaaac
    12721 acgtggattt gatcgtagcc ttgacaaaag cgaatcaatt agatgatatg tttcgttcga
    12781 tccaatccat tattactgag aatacttatg ttttgtgttt actgaatgga ttaggtcatg
    12841 aagatgtact ggaaaaatat gtaccaaaga aaaatatctt atttgggatc acgatgtgga
    12901 cagctggtct agctggtccc ggaaaagtca cattattagg tgatggagaa atcgaactag
    12961 agaacttgga accagaaggc gaagcattta caaaaaaagt tgtagaagtc tttcaagaag
    13021 caaaattgaa tccaatctat agtcataatg ttcgttattc catttggcga aaagcttgtg
    13081 taaacggaac attaaatgga ttatgcacga tattggattg taatattgct gaattaggtg
    13141 cgcaaaaagc ggcagagggc atggttcgaa cgattgtatc tgagtttgct tctatcgcag
    13201 caaaagaagg aatcacatta gatcaagaag aagtgtacca acatattgct tctacttatg
    13261 atcctgacaa catcgggttg cattacccat ctatgtacca agacctgatc aaaaaccatc
    13321 gtttgactga aatcgactac atcaatggtg ccgtttggcg aaaaggacaa aaatatgata
    13381 ttgctacacc atattgtgcc tttttgaccc aattagtcca tgcaaaagaa ggaatccttg
    13441 gcgcagaata aaaggctctc tgatgagaat cagctggatc agcagctggt tctcattttt
    13501 ttacccccat gaaagttcaa aaagaaaaac acaattctca tgttgccata agaattgcgc
    13561 ttgtttattt tcatctatga ttggttggca cttttattta tttttaacag aggaaagatc
    13621 gtattcatat tgttcataaa tagcggagtc agtagtgaaa aaacgttttg cccaattttc
    13681 tgcatcaaaa aattccaaca gttctgatgg gattggttga aatggcgtat gcaaaaattc
    13741 cggtaattca tctaaatcac gttcttgaag caaaaaaata ttttcagaat gataaaactg
    13801 ataatgagct atggcttgat tatctgtcac tagtttcttt tgaaaataca tagattcaaa
    13861 cgttcgcaaa gtcactcctt gctgcccttc ccgcaacagt tctagaatcc ccctcgattc
    13921 cccggttttt ttcagatatt cttcataagt aaggtatcca ggttgttcat ttcctcgaag
    13981 cggaagaata aagtaattgg tcgtaacacc tctttttgta aattcctgct tatatttttc
    14041 tgcccgttct tttctgccgt tatttgttgc accaaaaaat agatccgtct ttggttcgct
    14101 agaagaaaaa tccatatgtt tcgaataaaa ggaactatta tgttttagtc caagttcttt
    14161 cgcttctaaa aaatcaaaat gataaatctc atcgattgcc ggttcatctt gtgcctgctt
    14221 cagcatcgcc tcgtgttctg ctttaaagtg gttccaaaaa aataacacga tttttgcatt
    14281 ggtaaatgga cgcacgcgtc gagccatctc catatcaaat ccataatcta aaaaaacgat
    14341 cagctgataa tttgccaact gttgtcgagc tgatttaatg aattccgact gtagtaaatc
    14401 ttttccggac caaacatcta aagagcggat ggaaggaaaa aaataaagtg atttattttt
    14461 cgcaattagc aatacgtcgt ttttgttcat ctgttccgcc tcactttctc tgtcactttt
    14521 tcctatgata gcctgtttaa atagtaaaaa gccagtaaaa cgcttcctat tatttttata
    14581 aaaatttaac atttctatga aaatacgatg aaataaatca ttcttccatt aaaatatgcc
    14641 caaaagtttt ctgaatagtt gtaattatct gaaaatttgt tatcattagt cattattat
//
LOCUS       DACESX010000063        14237 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.063, whole genome shotgun sequence.
ACCESSION   DACESX010000063 DACESX010000000
VERSION     DACESX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.063"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            82..984
                     /gene="gnd"
                     /locus_tag="IXF88_001617"
     CDS             82..984
                     /gene="gnd"
                     /locus_tag="IXF88_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1508352.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            986..1987
                     /locus_tag="IXF88_001618"
     CDS             986..1987
                     /locus_tag="IXF88_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1508353.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1984..2268
                     /locus_tag="IXF88_001619"
     CDS             1984..2268
                     /locus_tag="IXF88_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1508354.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2301..3098
                     /locus_tag="IXF88_001620"
     CDS             2301..3098
                     /locus_tag="IXF88_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1508355.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3112..3945
                     /locus_tag="IXF88_001621"
     CDS             3112..3945
                     /locus_tag="IXF88_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1508356.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3963..4820
                     /locus_tag="IXF88_001622"
     CDS             3963..4820
                     /locus_tag="IXF88_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1508357.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4842..5351
                     /locus_tag="IXF88_001623"
     CDS             4842..5351
                     /locus_tag="IXF88_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1508358.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5388..5789
                     /locus_tag="IXF88_001624"
     CDS             5388..5789
                     /locus_tag="IXF88_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508359.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5901..6254
                     /locus_tag="IXF88_001625"
     CDS             5901..6254
                     /locus_tag="IXF88_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508360.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6288..6731
                     /locus_tag="IXF88_001626"
     CDS             6288..6731
                     /locus_tag="IXF88_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1508361.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6748..7833
                     /gene="uxuA"
                     /locus_tag="IXF88_001627"
     CDS             6748..7833
                     /gene="uxuA"
                     /locus_tag="IXF88_001627"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1508362.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7826..8539
                     /locus_tag="IXF88_001628"
     CDS             7826..8539
                     /locus_tag="IXF88_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1508363.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            9291..9491
                     /locus_tag="IXF88_001629"
     CDS             9291..9491
                     /locus_tag="IXF88_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1508364.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            10059..10622
                     /locus_tag="IXF88_001630"
     CDS             10059..10622
                     /locus_tag="IXF88_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508365.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(10683..11048)
                     /locus_tag="IXF88_001631"
     CDS             complement(10683..11048)
                     /locus_tag="IXF88_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508366.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(11124..11369)
                     /locus_tag="IXF88_001632"
     CDS             complement(11124..11369)
                     /locus_tag="IXF88_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1508367.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(11439..11693)
                     /locus_tag="IXF88_001633"
     CDS             complement(11439..11693)
                     /locus_tag="IXF88_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508368.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(11857..12180)
                     /locus_tag="IXF88_001634"
     CDS             complement(11857..12180)
                     /locus_tag="IXF88_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508369.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            12367..12440
                     /locus_tag="IXF88_001635"
     tRNA            12367..12440
                     /locus_tag="IXF88_001635"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12401..12403,aa:Ile,seq:cat)
     gene            complement(12825..13322)
                     /locus_tag="IXF88_001636"
     CDS             complement(12825..13322)
                     /locus_tag="IXF88_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1508370.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(13362..14171)
                     /locus_tag="IXF88_001637"
     CDS             complement(13362..14171)
                     /locus_tag="IXF88_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1508371.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT         4801 a         2286 c         2942 g         4208 t
ORIGIN      
        1 caaacaaaaa atatttttgt aaaatctatt tacaaaaaaa tttttttgta taatgagggt
       61 ataagaaatg gaggcgctat catggaaatt ggtgtaattg gtttgggaaa aatgggattg
      121 aatttagctt taaacttaaa agagcaccat cacaaagtac aaggactcga catttcaagt
      181 acagctgtta gtcaggcaag agaaaatgac attcctgctt atcaagagat ctctgaattt
      241 ctagctactt ttgagcagaa aaaaatcatc tggttaatgc ttcctgctgg agagataacc
      301 gaaaacatgt taagaaaact ttttccaaaa ctcggaccag gagacattat tattgaaggc
      361 ggaaactctt attataaaga ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc
      421 ggttacttgg attgtgggac ttctggagga atcgaaggag cacgacatca agcctgctta
      481 atgattggag gagataaaga aacttttcta tccgtggaac aattattcaa agatgtagca
      541 attgaaagag gatatctgta tgctggtgca tccggaagcg gacacttctt gaaaatgatc
      601 cataatggaa tcgaatatgg aatgatgcaa gcaatcggtg aaggattcca acttgtacaa
      661 aaaagtgagt atgcttttga cttggaacaa gtcgcacgtg tttggaatca tggttcggtg
      721 attcgcggct ggctgatgga aatcatggaa gatcagttaa acgaacatcc caatttaagt
      781 aattatcggg gaatcgtttc tgcatcaggt gaagcaaaat ggacggttga aacagcgcta
      841 gaaatggctg ttccagttcc agtaattgca actagtttgt tcatgaggaa tctttcacag
      901 gaagaagata gtttttcagc aaaagtagtt tcagcactaa gaaatgggtt tggcggacat
      961 gaaatagttg aaaagggtga gtaaaatgcc agattatcat atagcaatcg ttaattccag
     1021 cagtttcgga agagtctttc cacaccactt ggaaagatta aagtcagttg gagacgttca
     1081 tttctttact gttgatcaag agattccagg aaaagaacta gcggagttac ttaaaggatt
     1141 caatatcatt attgcaagtg ttacgccatt ttttcgacga gatttttttg aaaacaaaga
     1201 tgaactgcta ttgatttccc gacatggtat tggttacaac aatattgatt taaaagcagc
     1261 aaaagaacac cagacaatcg tatctatcat acctgcttta attgaacgaa atgctgtagc
     1321 agaaaataat ataactaatt tgttgtccat gcttcgacgc acgacacaag cacaagcaag
     1381 agtcaaggac gatcgatggg aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa
     1441 aacagttggt gtgattggtg caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa
     1501 cggttttcgc tgtgatgttc tagcttatga tccttacaaa tcagatttat acatcgaaag
     1561 ctttggtgca aaaaaagtag aacttcaaga attactggaa caatcagata tcatttgttt
     1621 atgtgcgaat ttgacagaag agaactatca catgatttct gataaagaag tcgatcagat
     1681 gaaagaaaat gtttatctat ctaatagtgc aagaggcgct ctagttgatg aagaagcgat
     1741 gattaatggt ctgaaaagcg gaaagatcgc aggatatgcc acagatgttt tagaagaaga
     1801 accgggacgt caagatcatc cgtatttagc gtttgaacaa gtagtgatta cgccacatat
     1861 atcggcctac acaagggaat gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga
     1921 acagatcagt cagcaccttc ttcctgaacg ttctgtccag acaaagagca actatataaa
     1981 aggatgatga agaaaatggt gaacggttta aaagtgaaaa caggccctca attttattta
     2041 tatgaagaag gtggtatttc aaaagtttct gatctcttga aatcatacgg cgcgaaaaga
     2101 gtactggtca cccacggaac ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg
     2161 aatgacgaaa cgattcaatt tttttatcat cgttattctg gagaatgtag ttatgctgaa
     2221 gccagacgaa tagctacaat catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg
     2281 gaggaaaact gaccgatcta gtgggttata gtgccaatct tgcaagaatc gatttcggag
     2341 tcatccctac tttagctagt aattgtgctc catggacacc tcttgctgtc atgtataagg
     2401 agaatggaca agcagaaggg aaaacagaac acttttacag acaagctgct ttttttataa
     2461 cagatcccca attagtgatt gattcccctg ttccatattt cattgccggg ttagcagata
     2521 cactagcaaa atggtatgaa tctgagctca tcttagaaca agattttttg caagatgaat
     2581 catttttgaa gctagcacaa gatactgcga agatctgcaa agaagagatt ttgaatcatt
     2641 ctgctaaagc aatccaagat atggcacaaa gaaaactgac atcggagttc aaacagcttt
     2701 cagagattat ctttgctgtt gctggtttag ttggaggatt aggagataaa tacgcaagaa
     2761 atgcggcagc acatgctatg catgacgcta tcagtaaata tattccggaa agccatcgtt
     2821 ttctacatgg agaaaaagta gcgtatggga ttttctatca actggcacta gaaggtcgct
     2881 gggaaaaaat agacgagtta tatcctttgt atcgggaatt agaactgccg gtttctctag
     2941 aacaaatggg attatttccg aaagataaac agataataga taaaatggct gaatttattg
     3001 attcgaaaga gaaagtgcat ttgattccta tagaggtaac acagaaatca ctgatacagt
     3061 caatgatgga actggaaggt tatatcagaa agatctgaag gaggaggaca catggaaaca
     3121 ttaagtgtag tacaaagttt gttgattgct ttatgggtag ccgcggttat gtcccgctgg
     3181 ttaggtggag gagccacgtt gaccttacgt ttttctcctt tgatgactgg attagtcgtc
     3241 gggttgatca tgggggaagt tgcacaagcg atgattgtta cagccgcttt gcaaatgatt
     3301 tatatggggg ttttttctcc aggaggatca atgccagctg aaccatctat tgcagcagct
     3361 atcgcagtac cagtcgcatt attaggggat ctgtcgccag aagcagcgat tgccgttgcg
     3421 gtgccagtcg gattgctcgg aagttacttg tatcagtttc gttttttcat caatactttt
     3481 ttaggtaaat atacagacaa agctgtagaa gatttgaata caaaagctat ggcccgatca
     3541 attattcttt atccaacggt tgcctcattt ttattattcg ttccgcttgt tttcattgct
     3601 ttatatttag gggcgccatt gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt
     3661 atccacattc ttgaagtagt aggtggaggg ttagcggcaa tcggtatcgc gacaactgta
     3721 tacgttatcg gtcgaaaaga ttatctggtc tttttcttcc ttgcttattt tatgagtgtt
     3781 gttttgaaat ctttagatat tacgatggtt acttatgcca tttttgggat tattatcgct
     3841 ttgatctttg tccagtcgca aaaagggaaa caagtacagc ctgccacaag caataacaat
     3901 tcagcatctt ttgatgacga agatgactat gatgacggat tttaatttag gaggaatggg
     3961 aaatggctga aataatggat aatcaaacag gtgctcctga agaaatcacg aaaaaagatg
     4021 taacaaaagc ctatctgaga tggcattttg caaacgaaat tcctcactct tttgaacgtt
     4081 acttggcgcc atcactacta tacggaatga tgccaatctt gaaaaaactt tataaagatg
     4141 aagactcttt aaaagcagca tataaacggc agttactgtt cttcaacact caattaagct
     4201 ggggtggcgg tgtgattact ggcctaatgt cttctatgga acagcaaaga gcagaagaag
     4261 agtatgaaga aaaagagatc ttgatgcagg atgacttgat gtataacacg aaagcgggat
     4321 tgatgggggc actagcagga atcggagatg caatcgattc aggaaccgtc caatatattt
     4381 ttatcgcaat cgcggtacct tgggcacaac agggaagtcc tttaggcgca ctttttcctt
     4441 ttatctgctt tgggttgtat caggttttac taggggtatt tttcgcaaga caagctttca
     4501 ctatgggccg taatgctaca ggcctaatgc aaaacactgg tgttcagaca gcgattgaaa
     4561 tgctatcagt tttagggttg tttatgatgg gggtgcttgc gggaaactat gtaaaagtag
     4621 aatcttcatt gaaatttgcg atatccggac gtgagtttgt tatccaggaa attcttgatc
     4681 aaatcgtgcc agggatatta cctttagctg tcgtaatggg tgtgtacttg ttctatacga
     4741 aaaaaggatt gaaagtcaca caagcattat tatggctgac tggaatatta attgtgctag
     4801 caggaatcgg tattttataa aaagaaggga acgtgaataa gatgggtgta gtcaatttag
     4861 cgcgtgtgga tgagcgtttg atccatgggc aagtaatggt aacgttatct caaaaaagtg
     4921 gagtgaattc catttttgtt gtagacgaag ttgtagcaaa agataaattt atgagagatc
     4981 tttacaaaaa tgcaggaagc cgtacaggac aaaaaacaat tgtcattaca ccagagaaag
     5041 ctaaattcta ttgggatgaa tatcaattta aagaatacaa ttgtattttg atcgcaaaaa
     5101 cagtccaaac gatttacgac ttagtaaaac acggaatccc catggaagaa ttgaatattg
     5161 gagggattgc tcaaaaaaat cctgatacag atcttttagt gacgaaatct gtttatttaa
     5221 ataaatcaga tgctgaaaaa ttgaaagaaa tgaatgaaca atatggcgta aagaatattt
     5281 attttcaagc cactccatct gcacctaaaa caggattaaa agatgtattg aacaagtttg
     5341 atttgtcata aacaaatgct tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg
     5401 agctgtttcg tgattgggaa tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa
     5461 agagtaaaca gcgttttctt tcagcattag tatcagatat ttacagtatg agaacagatg
     5521 tgacagtcat tgcttacgat acgctagcct accgatcaaa aaatatttat gttggagata
     5581 tcgaaaaagc agagaaagtt atctgtactt attacgatac acccgttcat gcattaggaa
     5641 gttatttcat gtttgattgg aaagaccaaa ggaaaaaaac aatctactct atattattgt
     5701 ccttcatttt gttgttcagt ttaggttggt ggggaatgat gatatataac aaaaatcctc
     5761 atcacgtatt cgatctgcta tccgtataaa caggtatcac tgtactagca tttgggagct
     5821 actttttctt attgggaaaa gcagctcgcg gatggcctag cagacaaact tttattcgaa
     5881 atacttcatc catattaacg atgctagaaa tgatccgtac gattgatgat ccgagtgtag
     5941 cgtatgcgtt tgttgatgaa ggctgttatg gagaaaaggg cttagacagc gtgagatcgg
     6001 gtatgaaaaa agaggctatt cttttttatc tagactctgt tggggcagat acaccgctac
     6061 agttttcggg aaattatttt tcaaataaag aacagtggct gaaacaagtt gataagttga
     6121 aagaaaaaaa cgtcaattat atttttagtg caagaaaaaa gcaagcgcag ttcttctatt
     6181 tgaccaaaac agatttacga gggaaaacat ttaattggca aaatgccaat cagattattg
     6241 ctctttttcg ataagtaaaa agtgcaaaac aaggagtgag aagactaatg ttggaaatca
     6301 tgatcgttac acatggtaag ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg
     6361 ggacatcgag ccatattgaa acaatgtgct tagagtctgg ggaggacatc caaacattcg
     6421 gagagaaggt caaagaaaaa ttaactatga tcaacacagc agaacccatg ctagtatttg
     6481 ttgatctaat tagcgcaagt ccgtacaatc aagcgctctt ggcagtcaat gaactgactg
     6541 aagaaaagaa agcagccact tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag
     6601 caatcaatca tcagatgctt cacacaccga tagaagaagc ggtggaacaa attgtcaaac
     6661 aaggaaaaga taatttaact gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg
     6721 acgattttta gaaggaaggc tgaaaagatg aagtggggat ttagatggta tggtgaaaaa
     6781 ggggattcga tcccattaaa atatgttcgc caaataccgg gaataaatgg gataattgga
     6841 actttgctga ctaaaatgcc tggagatgtt tgggagattt cagaaattga ggagttgaaa
     6901 gcatctgttg agagccaagg actggaactg cttggaattg aaagtgtagc gatccacgat
     6961 gcaatcaagg cagggacaga tgaacgagat tactatattg ataactatat acgaacaatc
     7021 aaaaacttgt ctcgatgtgg ggttcgattg atttgttata gcttcaaacc aatttttgga
     7081 tgggcgaaaa ctgatttgtt taatgaaaac aacgatggaa gctattctct tttatacgat
     7141 cagaaaatcg tcgatcagat ggagccagga gaaatgtatc aattgataca cagccagtca
     7201 aagggattca aattgccggg ttgggaagaa gaacggttga aaaaattcgc tcagctggaa
     7261 aaactgtatg aaggaatgac cgaagaagaa ttatttgaaa atctgactta ttttttagaa
     7321 aaagtgattc cagtgtgcga aaaagcagat gtaaaaatgg ctatccatcc agatgatccg
     7381 ccatgggaac tttttggttt tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt
     7441 ctaaatgcag tagattctcc atataatgga ctaacgctgt gcacaggatc tttaggggca
     7501 aatccggaaa acgatttagt tgaaatcatc catgaggtag gttcgcgcat ccattttgtc
     7561 cacttcagaa acgtggagtt tttaggagaa agaaagttca aagaaacggc acatccctct
     7621 acagaaggct cccttgatat gtatcatatt atgaaagcgt tggtgcaaga agggttcgac
     7681 ggtcctatac gccctgatca tggtagaacc gtgtggggtg aagtggctat gcctggatat
     7741 ggcctgtatg atcgtgcaat gggactcact tacatgcaag gtctatatga ggcgataagt
     7801 aaggaaaaac cactgggaga gcattatgaa taaaatcaag ctcttcatct ttgatatgga
     7861 tggcctttta ttagaaactg gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa
     7921 gtatgattat gaaatgcgta aggaagtcta ttatttacta actggccaaa cagaaatggc
     7981 gataagagaa caaatgggca ttttgtacgg agaagatgtt ccatatatca aatggcgaga
     8041 agcaatcaat caatataaag aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa
     8101 aggagcagaa gaaattttat cttttgcaaa agaaagagga atccatacaa tcgtggcttc
     8161 gtctaataca cgggtaaaag tagaaatgta tttaaaaatg gaaaatctat atgccttgtt
     8221 tgatcaaatc atttcagggg atgatgtaaa aaaaggaaag ccggaaccag agatcttttt
     8281 gaaagcttgt tcaaaaatga atattcctcc aagtgaagca cttgtatttg aagactcgat
     8341 tgcagggatc gaagcagcaa ggcgagcggg cattttgtct tttctcatca aagatcattt
     8401 gatagggttg cccgatcata atgggaagca taagttgaaa gtagatgtaa gtttatatga
     8461 atccaataaa gcagactatg aattttatga cctgcatcaa gcaaaacgat ttttagaaag
     8521 taaagggttg tatctctaat aaagaggact attttctagt cctctttatt tattgtcctg
     8581 cacgactgag ggaacagatg aaaaagaagt aattttttat tttgaagtat ctaccttatt
     8641 tgtagtgaac aatttagaac aaacacaaaa cgcatctact gttatctacc agtagatgcg
     8701 tttaaagaga agaacgagaa tcgtccatat gagggtttcc actccttatg cagatttttt
     8761 taattcttgc actgcttgtt cgatcgtaac acctgtcgct acatgattgg tattttcttt
     8821 gtcaaaaacg acaaatagat tcaaatctgt atccaaggtt actcgatatt gtatatcttt
     8881 gttatagaat gtcatagcca ttctcctttc tttaaatact tgctgaaagg caacgaacgc
     8941 gttgataacg aaattccatt tataagtata ccaccaattg aaaggttttc atagttaaga
     9001 aattttaaag tcaaaagttg cagataaaag caaataaaca agaaaacaaa aagtgaatca
     9061 atcgcggtta ttcaactttt tcttcgattc gaagacaata gaagacaatg aattttatat
     9121 ttcattgcat tatccttgtg ttattagaaa aagcatgata tattaaatga gtaatctatt
     9181 ttgaaacgta atttgagcga tatatctaca tagataaaac tccttttgcc tatcaaatat
     9241 ttattgcaac gaaaaaacgt acattgatac gtatcaggag gaatttattt atgaataacg
     9301 gtacagtaaa atggttcaac gcagacaaag gttttggctt tatcactggt gaagatggaa
     9361 atgatgtatt tgcacatttt tcagctatcc aaggtgacgg cttcaaaact ttagaagaag
     9421 gccaagcagt gtcttacgat gttgaagatg gtcaacgtgg ccctcaagca acaaacattg
     9481 ttaaagctta ataaaaattg acttttaacc gtcttttcga aagaaaagac ggtttttttg
     9541 tacgaaaaaa ttcattggtc gttgaatggg ctgaatagta gtgtataaaa caattttgtg
     9601 gagcgtgaag cttttatggt agaggaaaat acgaataaag tgatgttgat aaaaagattc
     9661 ctatcttgaa tatgaatcga gatgtcttga aaatgaatga gtttgaccaa tcattatcct
     9721 atcgaaattt acagtttcgt tcgattggtc tgtcaattaa tgaaaaagta aaaaagaata
     9781 tgttctagga agagggcggc acatctaaat ttttaagtaa cggacagttc aaatatcaag
     9841 cattgaaaga ggctatccta ctgtttgaag gaatacgaat atataaaaac gacaaagagg
     9901 aaaaagtagt tcattaaatc ccttcaagtg atgaaagaat gagatctaat aaaacctgat
     9961 ctttcatctg tttttttaat aacgtaataa tttttttatt ttttgtgctt tcgccgttat
    10021 tgtagagtga tttagcattc ttatttttaa aaataactgt gttatatatt aaaaaaggca
    10081 taatagttat aagctttttt aaagaatatt tagaaattat taattttcgt ctgaaaacaa
    10141 aaaaagaaag cgttattatt ttagtctatt ttcagggggg gaaccttatg ataaaaaaac
    10201 tatttttact atcaatatta acgctagcaa tggcaggttg ttctgactct ggcttagcaa
    10261 ctgaactgat cacaaaatca actgaagcca cgacagagga gttaaaatgt ggaaacgatc
    10321 acagtgacta cagaggctta aatcgacgat gcatgggaat attaggcaga agcaattgta
    10381 agcatcgatg tagtaatcga agaaggtgta gaagaagcaa cagaaaaatc aaaagaaaga
    10441 tcaaaagaag caatctgtgc tgtgcatcgt atacagacgt cgaagatgcg gtacttttaa
    10501 accacgtcag gagatgttca tttatgtcta gggaagttgg cgatgaacca aaactagagt
    10561 acgcttttag ttttgcacgt gaggatgaag ataaccctaa aacttacgac attttagctt
    10621 gatctaagat ggttaaagct ttttaatgaa gtaagaggag atagaaggct tttctagtta
    10681 tattaggtaa gataaattcc tttttgctcc aaatttgcta aacattctgc tagataaact
    10741 tcatcgtgtt ccggataaat cggcacgttg cgctctacag taggtaaatc aaaaaatgct
    10801 tcgcatgatt tgccacgata cataggattt ttccactctg gtgctgggct gtaaccacgt
    10861 ttttccattt catccatgac aagctgatga tattggaata atctataagg agaatagtga
    10921 aaaacataat ccacagtagc atgtttcttt ccccagccat ttcctcttaa tgcacagcat
    10981 tcccgatgtt gtcctaaaag ctgttgacga ggaagctttg aaatcaattt ttcatgccat
    11041 aatcgcatag aaatgtctcc tttatatata gattgttaac cttagacgtt aaatgaaaaa
    11101 accagcacca aggctggaaa aaatcacttc agtttgcttg ctgtttctga gcgaccaatt
    11161 aggtaatcaa cggaaatatc gtaaaagtca gcgatataga ttaattcaat gatgcttgga
    11221 atttcttctc cttcttcgta cttacgatat ttgaataaag gaatacttaa cgcgctagaa
    11281 acagctactt gagataaaga tcggccagtt cttaagaaat tcagacgaca acgtaaagta
    11341 tccagtaaaa tagcttcttc gatactcatt taatcacctt tcattatagt agaatagtta
    11401 taatgactat agaaagctac acatgcaatc aatagtactc aatgtttttt atttaaacgt
    11461 atcaaataaa gaatgacgat caataaagac aaaaccagtc ctgataattc ttctataaaa
    11521 acactatcgg gttcaaagta gttgtaatta aaaactttcc acataaagta gatagccgga
    11581 agagtcaaaa tagctaaata aaaccgttct ttgttccaat tcataagata aacaatactt
    11641 gcgaccagtg cgaccgcagg cacccaaaac aaaactctca ccaacgtaat catgtaacgg
    11701 actcctcaac gattcatata ataaaagata acatgttatt gcatacatca caaatgaaat
    11761 gctaggaaaa taaaagaaga atagatcaat gaaaaatgaa aaagcagaac agttttctgc
    11821 tgataagcag gaactgttcc gctgaatgtg tcttatctat cgagaaaaaa tcaatggatt
    11881 gatatctaag taattcaata catataaaaa gaggacgatc gcattcacta aaaaggaaag
    11941 aatgatgatc ccatagcgaa tggaaacata tttaacaaat acttttgttt gaatacgggc
    12001 atatcttttt cctttatagc tgagcatcat gacaagaagc aaaagggtaa tcacaccttg
    12061 aaaaatcatt ccaataaaca gaattcctga ggtttgataa tttaattgga gaaaataagc
    12121 tgctgccgca actaaatcga ataaaatcgc aataaatggc aaaataatca ctcctaacat
    12181 tgagaataaa taaaagaata acattttttc taaaataagc aaataaaaat taaagtaaca
    12241 aaatagttat gacacaagta aaagaaataa gaataacaat attattttac ttatattttg
    12301 aaacttgtga gagtttgtca ttcgtgctat agttgaaaag taacaaaaat aaattttatt
    12361 tatgatgtac ctttagctca gttggttaga gcagacggct cataaccgtc cggtcgtagg
    12421 ttcgagtcct acaaggtaca taagaggttg tgaaaaagct gccctgcatc aagtaataag
    12481 aacagccaag taaaaacagc ttcttatgtt tttacttggc tgttcttatt actgcagatg
    12541 caagcttttg aataccgttt aaggaagagg ttgtgaaatc agcgttttga cctgagtatt
    12601 agcttaacaa acggaaaaat agttcctcat attttgccgt ttgttaagct aataccgagg
    12661 gtcagctgat tgaacaccat ttaagaaaga ggtcgtgagc ctgccgtttc actctgtgtc
    12721 ataaaaaagc caatctactt tgatagaaaa agcacgtagg actaaaacag ttctgcgtgc
    12781 tttttttatt gtgtgttaaa tatcgtaaat gaagatagat atttttatga ttcgacgata
    12841 gagacgttga aatctttcaa cagctctaaa gcggattctc cgtgttttct actggagact
    12901 aacattggat caacaaaaaa gtctgcttca ggaaaagcac tctgcaggcc tatcagatta
    12961 gagagtacac acaagtttgt ttcaatcccg actacttcga tctctttttg ttcttctttg
    13021 ctttcaaata aagtttcctg gatttcagct aaagtttcag gtggaatcgt aaagtaatac
    13081 tttttaatca ctcgttcatt ttcttgtgga gaaagaagag ggatcaattg caaatcttct
    13141 cgttcttctt ctttgtcctt cagttcaact ggtatatccc gtgtgaagag aatatattca
    13201 ttattttggc gtgctttagc taatctttga gcaatacgtt ctgccaattg atctgactct
    13261 ggaatataaa attcgctgtt tggatcaaga atatgatttt gcatatcaat aataactaac
    13321 attttctttc ctcctacatt catatcgtca ttttattctt tttaatagaa gttttttaaa
    13381 atataagcaa caccagcttc ttcattcgtc agtgttactt ggtttgcaat ttcttttact
    13441 tgtcgctgtg cattccccat cgcaatactc aacccgctgt aattcaacat agcaatgtcg
    13501 ttttcttgat cgccaaaagc catgatgtca ctcgattcga ttccaaggta atccgccaga
    13561 aaaacaagtg aatgagcttt agtgatacct gtaggcgtga tttctaagaa atgcggatca
    13621 gagaatactg attggatacc atctgcttgc agatctcttt gtagcttctg tagtttttct
    13681 tcactcttat ctgttaatgc taatttaaga tagctaagag aaggatcttc caacatattt
    13741 aaaatttgat caaaagaatg gttctgtaaa ttgaaatgtt cataataagg gtcatctttg
    13801 actagatttg attcatcgaa tgtcagctga tcatcttggg tttctcggct agcaatgatg
    13861 gatattttcg cttcttgagc atagaagaag gcttttctca gtgtatcgct atctattttt
    13921 gaacgtctga gaactttcgc catttttcca tccttcattt cagcaatcag tgaaccattg
    13981 gaagaaatca aatagccacc gattcctaat tcggaaaaaa tatctttagc agaaatgctg
    14041 ttccttccgg tagcgacggt tacgattccg ccattttcat caaatttccg aagtgcttgg
    14101 atattttctt ctggtatctt tccatttcga tcaagcaagg taccgtctaa atcaatcgct
    14161 gctagtttca ttttatcttc ctactttctt tttacatatc tattttcctg ttaccttaag
    14221 attaaatgag aatttaa
//
LOCUS       DACESX010000064        14063 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.064, whole genome shotgun sequence.
ACCESSION   DACESX010000064 DACESX010000000
VERSION     DACESX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.064"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..249)
                     /locus_tag="IXF88_001638"
     CDS             complement(<1..249)
                     /locus_tag="IXF88_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1508372.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLI"
     gene            complement(848..1567)
                     /locus_tag="IXF88_001639"
     CDS             complement(848..1567)
                     /locus_tag="IXF88_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1508373.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1616..3262)
                     /locus_tag="IXF88_001640"
     CDS             complement(1616..3262)
                     /locus_tag="IXF88_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1508374.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3441..4031
                     /locus_tag="IXF88_001641"
     CDS             3441..4031
                     /locus_tag="IXF88_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1508375.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            4123..5628
                     /locus_tag="IXF88_001642"
     CDS             4123..5628
                     /locus_tag="IXF88_001642"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1508376.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5712..7064)
                     /gene="celB"
                     /locus_tag="IXF88_001643"
     CDS             complement(5712..7064)
                     /gene="celB"
                     /locus_tag="IXF88_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1508377.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7064..7582)
                     /locus_tag="IXF88_001644"
     CDS             complement(7064..7582)
                     /locus_tag="IXF88_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508378.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7598..7924)
                     /locus_tag="IXF88_001645"
     CDS             complement(7598..7924)
                     /locus_tag="IXF88_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1508379.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7937..9916)
                     /locus_tag="IXF88_001646"
     CDS             complement(7937..9916)
                     /locus_tag="IXF88_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1508380.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9937..10251)
                     /locus_tag="IXF88_001647"
     CDS             complement(9937..10251)
                     /locus_tag="IXF88_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1508381.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10419..10712
                     /locus_tag="IXF88_001648"
     CDS             10419..10712
                     /locus_tag="IXF88_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1508382.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10790..11902)
                     /locus_tag="IXF88_001649"
     CDS             complement(10790..11902)
                     /locus_tag="IXF88_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1508383.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            12289..12468
                     /locus_tag="IXF88_001650"
     CDS             12289..12468
                     /locus_tag="IXF88_001650"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508384.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12662..12832
                     /locus_tag="IXF88_001651"
     CDS             12662..12832
                     /locus_tag="IXF88_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1508385.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13256..13996)
                     /locus_tag="IXF88_001652"
     CDS             complement(13256..13996)
                     /locus_tag="IXF88_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1508386.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4395 a         2867 c         2413 g         4388 t
ORIGIN      
        1 gatgagaaat gcttgatccg aagaaaataa aaatttgaaa ttttcccaac ccacccaagg
       61 actttccctc aagctagcca agaacccatc tgctgaaata tgaaaatttt tgaatgctac
      121 gacattcgcc attacaggta tgtaaaagaa aaaaatcatc caaagcatac ccggtacagc
      181 catcaggaca agtgctttat aacgccatag attcataaaa aaatgattga acccctttcg
      241 ttttttcatc taaaaacccc tttcgattca ttcgcaacgt aattatagcg ctttcaataa
      301 tgagaataaa taaacaaagt atagggaaaa tgaaacaaag tataggagag gttgtgaaat
      361 cagcgttttg gcctgagtat tagagaaaaa aacgggaaaa tagttcctca tattttgccg
      421 ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcagag gttgtgaaat
      481 cagcgttttg gcctgagtat tagagaaaaa aacgggaaaa tagttcctca tattttgccg
      541 ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcagag gttgtgaatt
      601 cagcgttttg gcctgagtat tagggagatc gtgtccttac tatctaactc tgagtcacaa
      661 ggaacaatgg ggactgtaac aaaagtcttg tcacagtccc ttattccgaa caaacggtgt
      721 tccagaagta gcttctacgg aaataagccg aaatttcaca aaaaagccat ccgaaagtca
      781 gacggacaag tctgcttttc agatggcgct acaactactc tgcttattct tttagctatc
      841 cattcaatta tttttctctc acttgttcta gctcttcttt ggtcatgggt cgatattgcc
      901 ctttattcaa atctttaggc agccaaaatt ttcccatacg gattcttttt aaatataaga
      961 cttcttttcc tactgctttg accattcgct taacttggtg aaactttcct tcatgcaaaa
     1021 tcagacggat aattgaagtt tctgtctctt gatctacttg atcaatttgg agttttgccg
     1081 gcaatgtctg ctcatcgcca tcaataatca gtccttctgc aaataaacgg atatcttctt
     1141 ctgtcattat accgctaacc tcagctagat attctttctc cacatgtttt ttaggtgata
     1201 gtaactgatg ggcaaacacc ccgtcattcg ttaaaatcaa gaggccctct gtgtctttat
     1261 ctaatcggcc gactggaaaa agatcttccc gataatcctg gtcttccaat aaatcgatta
     1321 cggtttgatc atgattatcc tgagtagcag aaatgactcc tgcaggttta tgcaaaatgt
     1381 agtaaaaatc tttttgatac gtaatgattt ctcctaaata agtaaccgca tcttcgaatt
     1441 catttacttg cgctttatcg cttttcacca cttgtccatt gacttcgatc gttccttttt
     1501 tgattaatcc tttgacttct ttgcggctgc caagtcccat atctgctaaa aacttatcta
     1561 aacgcatgcg tttcccccat ttcaaaagag catcaatcta gctcttctga ttcacttatt
     1621 ttatttttaa tttatgtcgt aaacgcgcta ttcctggtcc tagtagtctt tctgccaagc
     1681 ggattttcaa agctaaataa ccatacacta aagcccctat acctgctact acaagaatca
     1741 acaatagaga ctggaatttg ctatcttcgt ttaaaaaagc accaaaaatc atctttgtga
     1801 tccacgaagc caaaagcata atcaaggtaa gaataaagat cagcaatgtc ctgcgccaga
     1861 caaaacttgg tttaaagcgg gctacttcat ggattctctt taagatcaag gtacaagaca
     1921 cagcaaatcc gatcattgtc gccaataaag ggccgtatac ttcaaataaa cgaatggctg
     1981 ggtattgaag aaggagtttc actagaaacc caataaccaa ataattaatt gccgccttgt
     2041 tttcaaacat tccttgcagc atgttggaaa ctagcatata tagtcctagg aagagtccta
     2101 caaaacttgc ttggatcagt acattacttc ctagagaatc cggtgtataa aataatgtat
     2161 ttagcggata ggcaagtaac ataacaccaa atgtcgccgg aaacataaaa aatgaaaaca
     2221 actgtaaatt tccgcttgtc aattttgcca gacctactcg atctttcaac gtaacagctt
     2281 ctgtaatcaa cggtaaccca gtagacgcga tcgatgttgc caatgcaata actaccatcg
     2341 tcaacttatc tggattggca ctaaaaatcg aaaaaagatc cagtaactgt gcattggaat
     2401 actcggtgga tgcagacatg atcttcataa aagtgaactg atcgactaat ttgaagatcg
     2461 ttacccctga acccacaata ataaacggaa tcgcttcttt aacggtatct aacaacaatt
     2521 ccttcgttgc aatatttatt ttgttttcgc tgtagtgaat aaacgccgat gtatacgctt
     2581 gatgtttctt caaaaaatac aaaaggacaa gaatacttgc tagcataccg ataaatgccg
     2641 cgaaagttga ttgcgtgacc gccgtcacat agtcaccatc catgactttc atgatgatga
     2701 atgtagccaa tagcatataa aaaatacgtg ccacctgttc tacaatctgc gacagcgcat
     2761 aaggcatcat ttcttgattg ccttgaaaat accctcgtat cacactcata caaggaaaaa
     2821 ccaatacagc gatactcaaa gaacgcatcg ttggaatcag ttcttctccg ccaccagaag
     2881 catgagctag ccaaggagag gcgatgtaca tgaacaaagc aaacaacagg cctaacccgc
     2941 ccatcatctg taatgcacgg ataaataatc gtcgtgaagt gccatattca ttcaatgaat
     3001 tatagcgtgc agtttgtttt gcaatcgcag caggtattcc tgctgtagag atcagtagaa
     3061 acaatgcata tatattgtat cccatattga ataatccatt ggcagcctta gcattttcgc
     3121 ccatccaagc ataccaggga atgatataga cagcgcctag caatcttgaa atgatattac
     3181 tggcggtcat ccaagcagag cctcgtgcca ttttttcttg cggtgttaat tgatctaatg
     3241 ctggttttct ttgattatcc atcggtcaga ttccaacttt ctacaaaaca ttactatcat
     3301 tttaattggt aaaacagaaa ctggcaatcg attttatata acttttataa gaacaattgt
     3361 ttttttgaat tcggtttcgt ttttttggta aagtaaagaa atatacagcc ttttatggct
     3421 aacatttata ggagatatca atgacacaag aaataaaaaa actacccgtt atatttttag
     3481 gtcttttctt tatcacagtt ggattgaatt ggttcttgct ccctcacgat atagcttccg
     3541 ccggagtggg cgccatcgga catttagttg aaacggcctt ttctatccaa cgctcagtta
     3601 ctgtttggag catcaatctt atgatgctgc ttctagccgc tgtcctatta ggaaaatcag
     3661 tattttttaa gactgtagcg ggaagtctgc tttttcctgt tgttttagag attgttccaa
     3721 aagtggaaat gatctcctca catttctttt cactgatttc aggaagtctt ttgttcagtc
     3781 ttggggttta cacattgtac acagtcgggg cctcaaatgg cggtataacg ataccgccga
     3841 ttattttcca caaatttttt cggcttccaa tgccgcaagg acttttactt accaatagtc
     3901 ttatcgtctt tttgaattat tatgtttttg gattgttgga gacactattc gttctcctat
     3961 ctattacagt cagttcattg tttatgaaaa tactcatccg cttgtcccct gtgtcaagaa
     4021 aatttgaata aagctggatt ttgtcatagc ggaaacaaac gatatattaa aaagagtgtg
     4081 ctataataaa ccaagaaatt gaataaagcg agggatcaat caatgagctt ctctttagaa
     4141 gacatccgtc atttactatt aaaagaaaat ctattaaaag aatttgtttc aacaagaggc
     4201 tggcatcttg atgtaccaga aaaagctgaa tttcttcagt taagttatga ttcaagaaaa
     4261 gctgatgctt ctactttgtt tttctgtaaa ggaatgaatt tcaaagaaga atatttggat
     4321 tcagcaatcg aacagggtat tcaatattat gtatcagaac aaccatacga aaattcagca
     4381 gtaggaatca tcgtaacgga tatccgtaaa gcaatggcgc ttcttgctat ggctttttac
     4441 gactatccgc aaaataaatt gaaagtgatc ggattcactg gaacaaaagg taaaacaact
     4501 gctgcgtact ttacaaaagc aatcttggat cacacaacaa ataaaaaaac agcattgctt
     4561 tctactatga atacaacgtt agatggcaaa acatatttca aatcccattt gacaacgcct
     4621 gagtcattag atttatatcg tatgatgtca gaagcagttg aaaatggtat gactcatcta
     4681 gtgatggaag tttcttcaca agcttacaaa acacaacgcg tatacggctt gactttagat
     4741 gtcggaatct tcttgaatat ctcgcctgac catatcagtc cgatcgaaca tccgacattt
     4801 gatgactatt tctattgcaa gcgacaattg atcttgaatt caaaaacagt tgttttaaat
     4861 catgaaagtg actattttga tttacttagg gaaacagccg atctattcca gattccaacg
     4921 atcacttatg gtcgttcgga agaaagcaat tatcaagtga tccgttcaga taaaggtact
     4981 cacggtttta ctttatcttc tcatgaagat cgcttagcta tctgcgatac tgcttatgat
     5041 attttgctgg ctggaggatt caaccaagaa aatgcagcca gtgcgatcat tgccgcagca
     5101 ctaacaggtg cctccagtca agatgcacaa gacggattaa aagaagctcg tgtaccaggc
     5161 cgaatggatc aattgatcca atccaatgga gcgcatgtct atgtggacta tgcacataat
     5221 tatctaagct tgaaaacatt gctgaaattt gccaaaaatg aacatccaga tggccgtgta
     5281 attgttgtat taggcagccc aggaaacaaa gcaatctccc gtcgacatga ttttggaaaa
     5341 gtgctatcag aaacagctga catcgccttt ttgacggcag atgacccagc atttgaagat
     5401 ccgaaaaaaa tcgcggaaga aatcaatgaa gcaattacta atccagattt gatcgttcat
     5461 tatgaaatgg atcgtcctga agcgattcga cgcgctttag cagaaagcgc tccgcaagat
     5521 tcagtcgtta ttgcgggaaa aggcgtggat ccctatcaaa aaatcaatgg tgtagatgaa
     5581 ccttatgaag gggattatgc aatcgtgaag cggttgattg aagaataatg gtatttatat
     5641 actagtggtt tcccatttct aagaaaaaaa gggactgtga ttttttgtca cagttccttt
     5701 tttcttagaa atcattttaa cttttcacta gctaattctg ctgccttttc tctttcttca
     5761 tttaataatt tattatcata gaatttgata aatgggaacc agataatgaa tgcgatgatt
     5821 gcacaaatga ctgctaacac agcagctttc caatcaccac cactaccaac aaatgctccg
     5881 atcccgactg gtgaaggcca cggcatctgt gcaagcatcg gtttgacgat ttccagccga
     5941 atggcaaaat aagcgagtgt agcagaagcc atcggagcta agaaaaacgg aaccgctaaa
     6001 tagggattgt agacgattgg catcccgaaa atgattggtt cattgatatt gaataatcct
     6061 ggtacaagtg aggcttttcc taacactttc agctgatcag atttcgcgag gaatgcaata
     6121 aagatgatca atcctaaagt cgcacctgat ccaccaacgg ttacataaga attgttgaat
     6181 tctcctgcca acggataatt tgcgccttgt gcgttagctg ccatattagc taatacgatc
     6241 ggggtcaaga aagaagtgat gatattcgct ccatggatcc ctacgatcca caatgcatgg
     6301 atcaagaaat aaatcaccat gacaccaagc catgtattcg tcaactgcgt aacaaaccca
     6361 aatgggatag caatcatttt gaagatatcc gttcctagtg caactaataa tccattgatg
     6421 atcatcacca caaacgcaat gacaaatgtt gggatcagag cagtgaaaga acgagaaact
     6481 cctggcggaa caacatctgg catttttatg acccaatttc tcttgacaca catacaatac
     6541 aattcgaccg caataatcga cataataata gctgtaaaga ttcctgaagt tcctaaacgt
     6601 tcgacaaaag agcccatcct aataccattt actacagttt ctgtctcatt gatagaagca
     6661 atcaagctga ttttaccatc agtcatgatc aattcaggga tacacataaa gaatgcaaaa
     6721 acagatagta atgcccccgt gagcggatta acatttaatt tttgttcatc tcgttcaatt
     6781 gacgttaatt cataagcaaa cacaatggca aagtaaatag ataaaatccc catcgtcatt
     6841 gtattgacga tcatgtacaa gtcactaact ttgaaaaaag ttgcattaaa aaatccttct
     6901 aaaaatggga acggtaatgg caaaatattt aaaaccaaaa acatcgagcc tacaatcgtg
     6961 aaaggaatag aagccatccc cgctgccatg acagcccgaa cgattttaag ttgcgcaatt
     7021 ttccccatag gtcccatcag gtatttctgc aaaaaatcaa acattagtta ttctcctttc
     7081 ctaaatataa cgctttttca tattgtttaa tacggatata ctgttggtct tggttgagtg
     7141 aaattttctt taaacgatac tgtataaatg cactggcaaa aataccgctt acaacaagaa
     7201 tgaaaacaaa taactgtcct ccattgttat ttgccataaa aggatacaag aatgtaaatc
     7261 ctgaggtaaa caacactgga atcactaata aattaaagac actctggaac aaaaaatacc
     7321 actttgtcca tggaacgttc gtttggtgtt ttcgataaag ctttacttgt tctcctactg
     7381 caggcaaaat agctaaaatc agaatcatcg gtaaactact tcctaaactt ttagctgata
     7441 gaagcagaat catccattgc atgttcatga aaaagaaaaa ggcagtcagg taacgtacga
     7501 ttagataacg attgaaatac atgtttttca aactaagttg ctttttttct aattcgattt
     7561 tcctttttac tgtatcctgc acaatgatcc ttcctttcta tttatttttt tgatacaaat
     7621 aattcatttc ctgtgcaact tccaataatg tcatcgtcgt catcagatga tcttgtgcat
     7681 ggaccatgat aatttccatt tctatttttg tcccgctggc atattctttc agaagttgag
     7741 tttgtgactg atgcgcatga agaatctctt catttgcttg tcgcaacttc tcttctactt
     7801 cttcaaacat ctctttacgc atcgagcgaa atgcttcatg tatcagtgtc cgtgcatttc
     7861 cgctatggaa aatgatatca aaagaagtca cttgtagtgt ttcactgttt attttttcag
     7921 ccatctgttt tcctccttat tcgatcagtt tgataaattt actgctgaaa ttctcaaaaa
     7981 taggttcagc taagatttcc tgctgtactt ttagctggtc aactaaccta acgattgctt
     8041 tagtcacgac cgtaattcct tcattttcga cataagaagg cgaaactaaa aaaacaaaat
     8101 gaatttcttg atattgatcc cactgcatcc cagaaggaat caatgcaacg gctacctttg
     8161 tttcgattcc aactggaaca gcgggatgtg ggactgccac tgtatcactg aatatgattt
     8221 gtcccatatt ttcacgttgc tggatctgct ttcgcatttc tcttatatat ccttcttctt
     8281 catttatgct cagcttttgc agcaaatcat gaagaatttc ttcctttttc ggttcgtacg
     8341 tataagtctt gaaaaatttt tcagacaact gttcggtgta aatctgcttt ttttgctgta
     8401 gagaaaatgc tggtatcggt ctatctgagg aatgataggg tatagattct ccaactacct
     8461 ttcgtatttt acgaacatca tcttcattca aaaaaatact gacatgtaaa actggaattt
     8521 tgaaaaacat tgtcgaaaga tcaatagaag aaataatcag gtcgattcct tttaaagttg
     8581 tttcattgat ctcataatag ccttttacac ttaccactgt aatattttta ccgaattcac
     8641 tgactacgcg attctttaat agttgggcac tgccgtaacc cgttgcacag ataatcaatg
     8701 catgtagttt atgcgcagct ctttcttttt ccaatgccgc catgagatgc aaagccaaat
     8761 aggcccattc atcttcattg atggtatagt ttttcaaagc cggcatgttg ctgaaatgcc
     8821 tttttactgc gtcaaagact ttgggatttt ctttctttat ctcctcagtc aatgggttct
     8881 caatgcgaat acctcggctc aatcgaatca tcatcggtga caaatgatcg agtagtccat
     8941 tgaaaagctg gagatcctct actaatgaaa aatgaaattc ctgagacaat cttttcagaa
     9001 ctttcgctaa ttcctttgat agttcctgat cattttgatt ttctttgtga ttcgacttag
     9061 ccatgagatg taatgtcaaa taagcaattt cttcttcggg aaacgataca cctgcgatta
     9121 cctcaatacg ttgaacgatc cgctgcgcca cttgatactc tactcgctta gaaacttctt
     9181 gttcgatgcc taattccttg atttcaaacc cttctcggag ccgtttgatc cccaaagcca
     9241 agtgtaaagt cagattctgt atgataaaat ccgaaacttt taacttagct tctcttgttt
     9301 catcgagaat gataatgact agctcttcaa aactgatgtc tgggaaaaaa cggctttgcc
     9361 ctaaatattc tttcaatgaa tttccataat ggtttccaaa aaaagtatcc atgataaaat
     9421 gccgtcgatt tctttcttcc ccttcgatcc atgtcccttg accatgctta gaaacgattt
     9481 ttagtgaata tggaatcaat cgctccttga tttcttgcat gtcctttgtc aaactggaac
     9541 gactgatata aagctcttct gccaaatcat ccataaaaag aatggcatct tccaaaagca
     9601 attggttcaa tatatacttt tgtcgatctt cggactcata aaatccttgc ggttctctag
     9661 catatccagg tactatttct tgccgactca aaaacaagtc aaatgtcagt ctttgtaaaa
     9721 tatgcagttg gtagccataa cctggtttag caatgatctt acctccgttt ttttctacta
     9781 actctttgat ccgctgcaag taattacgga ttgtccgatc agataataac aattcagaag
     9841 ctaaacgttg actggtgaca tactccttct tgtgatcaat caaatatctc aacagcaatt
     9901 tttccttctg tttcattatc atccactcct attccatcat atgttctctt cgatcacctc
     9961 tgctaacttc tggatgcctg tagggattgg gacatacgct tggaaaggga tactaacaac
    10021 aggctttcca acagattctc ctaatttttt gaatttatcc aaaaacatcg tcgtttgcgg
    10081 gctgatcaga aataaatcaa aatcactatt cttaatcata ttatcgcctt ctgtagctga
    10141 aactgcatct acgagtacct cttctctttt ttgcttaaag aaatccgttg tctttgaggc
    10201 catcatcgag gatgacattc ctgccgcaca aataatcaat gcttttttca taaatcaatc
    10261 actcctttta tttttataaa ccaagcatat cttgtaagcg ttttctattc aaaatatata
    10321 tttccgtagc attgcggaaa aaatgtgtat catatcttga ataataattt ctggcaccgt
    10381 gatatactag tgatagaatt ttttcaggag gggatttagt gagtatcaca gtgaatattt
    10441 tgtatacaag tacaaatgga caagccagaa aatttgcaga agaaatggtc gaaagaaaca
    10501 ttgtcaaagc gattcgtgca gaagaaggaa acgagaaata tgactattac tttccactag
    10561 aagatccaga atctctttta ctgatcgacc gttggaaaga tgaagaagca atcgaaaagc
    10621 atcacaaatc cgaaatgatg gctcaaataa acgaactccg taaaaagtat aaattaaaaa
    10681 tgaaagtcga acgtttcact gatttaccgt aaaaagatac acatggaatg atagcaattt
    10741 atcatccatg tgtatcttca ctttttcctt attttttatt cgatctcatc taatgattca
    10801 aaccgtgttt ttctcttatt tttcggataa gtatttcagt caccccaata tacatgattc
    10861 ctaaacatac cccaaggata ttctcaaaaa tgcggatcgt tactgctcct ggaaccccga
    10921 ataaactggc agtgattgcc aaagcaccaa aagaattaaa aactgtttta tatctatagg
    10981 tagaacaaac acctaaagct agaccaccta atataccgac ccagttaaaa ggaataaatt
    11041 ggctgatacc aataaataaa acacatccaa tcaatgagcc aatgattcgg tctactgctc
    11101 gttctttgaa accaatcgac atcaatccat agctagatac aatagaagaa aatgcaaacc
    11161 ctgcccacat caatcgctga aaagggacat actctcctgc aacgatcaaa agactaatgc
    11221 caaatgcata gatgagcatc caaatttttt cctgcgaata catgcctttc aaaaaaatct
    11281 ctttaaacaa gcttttgtct ttgttctttt ctcgatgttt tctgtaaagg agaactgaaa
    11341 accaacaaaa aaacaaaaat aataagctac ttgtttgtgt aaagaaattc ttgttcaacg
    11401 aatctttagg taacgaataa acaataaaga gatatgaaaa gccgtacaaa gaagcattcc
    11461 ccattttggg atcactagcg gttgccatta atatgctgct tagtaaaata aaatgaatca
    11521 acatgcccat aactgaccat ttgatcagct ggacatacgg tgctactaat agtgagaaca
    11581 tgacgattgc taaacctatg atcgaatggg aaatcttata accaaagtca acaaaacgaa
    11641 tactcatcaa catacagaat aaaaccacag cgtatggttt atgcttcgat tcaaataatg
    11701 ttgtaattat aatgataaaa caaatggcaa acaatacgat gaataatgat cgtaaaaaca
    11761 atgaacaaat atacatgcac ttttcttttt tagaagctgc ttctcgtatt ttttgtttta
    11821 aaataaacgg atctagttgt agagactgat aaaaatccac ctgttgtcgt cctttctttt
    11881 tacttttatt ttttacagtc atctatgaca ggttgtaaat actatggaac atcatatggc
    11941 gttgtcattt tcttgtcaac tatttttcct attcatgttt ttataaaaaa acatatcgtt
    12001 ttcttgtatg attaaatcac cacaaaataa acaactgaga gaacgatatg ctttatgaat
    12061 ggttctatca aaaaaattct gagatcaata gacaaaaatt tgatgattac agaggtctct
    12121 tacgaaacct tacagaagaa aaaaacattg atcgttgctg cagttctctt ccctgctcca
    12181 tgtgtctgta gaaattgtgg ttctgctgtg gtagatggaa ataggaaatt aatggtggtg
    12241 aaaaacaaga agaaggaaac cattgtccac tttgaataat ataatcatat gccaatggtt
    12301 atacgcctaa aaaagcagcg ctatacctgt aaaaactgtc gaagccattg gaacgctcaa
    12361 agttatttta tccgtccaag gcactcgatt tcaaatcatg tcagacataa aatcacttct
    12421 ttactgactg aaaaagtgtc gttatttttt atttcgaaaa gttgttaggt atctttgaca
    12481 accgttattc gcacattgaa agagttgaga agctatttac caaagtcttc caaaagaatt
    12541 ctgtcttgag tgttgagggt tgtgaatttc gttctcacgt ttccgttgag gataaactga
    12601 gttttatttc cgcggatggc gagacagaaa aattaatcga gaaaatttac ctacacaaaa
    12661 aattcctcga ttaacaaact atttccttag ctgcactaat cctaaagaaa tagaattctt
    12721 agttacagac atgaacgcca cctactttca gctcaccaaa catgttctac caaatgcgaa
    12781 attggtgatt gatcgattac ttggtttttc cacgcctttc aaggaggcgt aacctttatt
    12841 cacgagttgg ttgaagcttt ccgagacaaa agacccgact tgtttttctc cttattgaca
    12901 aagagccatt aaaactagag aaaaaattac aactgatcgc tgattatttg tagaaagaaa
    12961 atcaaagtat tacccaataa acttgagtca ttaggcaaaa aacactgcga aactcaagaa
    13021 tgattttcgc agtgtttttg tatatttttt attaactagt attactacaa aggtttattt
    13081 ttcacttctc aacgcagcac aaaattgtct ttacttattt actggttggg ttcaaccttc
    13141 tctactccga tactaatatt tgtttgtata ccccttctaa aatcggtagt ttccattcaa
    13201 ttgatgggta ctagttgatt ttaaaaacta tgcttattca attttttatt ttagctcata
    13261 tatttcgttt ctttctaaat tttatactga tgaaaacaag tagcacaccc aatattgaca
    13321 atataaactg gctaatgatg gtttctccag tttttggaag atgcttattt ccactgcttg
    13381 ttggattgtt tcctcttata tctgagctat cagcaacttt tctattctct tttgttaaaa
    13441 agatcgccac ttcttgttct ttttcgatct tgaaagttac tggtgtcgca tccaattcat
    13501 accccgttgg cgcttgagtt tctaccagct gatatgctcc tggtgctaag ccatctatcg
    13561 ccagttttcc atctgctcct gtggttagac ctgactgcag aacggttcca ttggcatctt
    13621 gtaactcaaa aacagctcct tgcaggactt ctccgctttg gtcatcgatc tttgtcaaca
    13681 cgacaccgcc tggtgtaagt cgattttctt tcgtcaattc taccaccgct gtctgacttc
    13741 tttcgatctc aaactcgatt ggtgtcgcat ccaattcata ccccgttggc gcttgggttt
    13801 ctaccagctg atacactcct ggtgctaagc cgtcaatcgc cagttttcca tctgctcctg
    13861 tggttagacc tgactgcaga acggttccat tggcatcttg taactcaaaa acagctcctt
    13921 gcaggacttc cccgctttgg tcatcgatct ttgtcaacac gacaccgcct ggtgtaagtc
    13981 ggtttgcttt cgtcaattct accaccgctg tctgacttct ttcgatctca aactcgatcg
    14041 gtgtcgcatc taattcatac ccc
//
LOCUS       DACESX010000065        13974 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.065, whole genome shotgun sequence.
ACCESSION   DACESX010000065 DACESX010000000
VERSION     DACESX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.065"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            2..3190
                     /gene="fss3"
                     /locus_tag="IXF88_001653"
     CDS             2..3190
                     /gene="fss3"
                     /locus_tag="IXF88_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1508387.1"
                     /translation="MLSLAVQSIVGAIAPTIAFADEITHPQTVTVELDLAHQYAVEGT
                     FSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKNPLPDMSDKAKLVS
                     VLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAIEAKINQAIADYQK
                     KPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVNGNQLIISPNANSK
                     ESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVEMEGSLKIKKVDKE
                     SGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTITEKSVPAPYTIDTT
                     PMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSLAGNTFAIRKDSPT
                     GEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTFKPVKVELNYVNQT
                     VALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTLFTVKDGKAVKWTE
                     NFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAPEGYTLDETKYPVS
                     IKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGFNDLTFKVSPLEGT
                     NEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGFQKITPLEIRSTFK
                     ENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNRLMLYDLPEEEDSL
                     TSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDVDATKAAQEKDKKA
                     KPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYESKEAYEAGDEPVA
                     KDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFDDVSITHDVLDGSK
                     EAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDTSKYPEGTSFTFKE
                     INYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVPSGSQESSPTVKAF
                     PQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3302..3616
                     /locus_tag="IXF88_001654"
     CDS             3302..3616
                     /locus_tag="IXF88_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1508388.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3629..4003
                     /locus_tag="IXF88_001655"
     CDS             3629..4003
                     /locus_tag="IXF88_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1508389.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4013..4357
                     /locus_tag="IXF88_001656"
     CDS             4013..4357
                     /locus_tag="IXF88_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1508390.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            4426..5442
                     /locus_tag="IXF88_001657"
     CDS             4426..5442
                     /locus_tag="IXF88_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1508391.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLSALFIGWMGVFYFPELRQFGL
                     SKVSFSTIEIAWSMKDLVCLLGSLALSGSMMLLYIHFFPDHWRSLWNRQKLARMILEN
                     HWYEVKQTQSDGFFKDLNRSRTKETISYFPKVYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISKCVEDFYERMMARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6036..7910
                     /gene="ltrA"
                     /locus_tag="IXF88_001658"
     CDS             6036..7910
                     /gene="ltrA"
                     /locus_tag="IXF88_001658"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1508392.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8036..8392
                     /locus_tag="IXF88_001659"
     CDS             8036..8392
                     /locus_tag="IXF88_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ1508393.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            8437..9579
                     /gene="dcm"
                     /locus_tag="IXF88_001660"
     CDS             8437..9579
                     /gene="dcm"
                     /locus_tag="IXF88_001660"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1508394.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9569..9858
                     /locus_tag="IXF88_001661"
                     /pseudo
     CDS             9569..9858
                     /locus_tag="IXF88_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10114..11298
                     /locus_tag="IXF88_001662"
     CDS             10114..11298
                     /locus_tag="IXF88_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1508395.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11313..11408
                     /locus_tag="IXF88_001663"
     CDS             11313..11408
                     /locus_tag="IXF88_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1508396.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            11437..11658
                     /locus_tag="IXF88_001664"
     CDS             11437..11658
                     /locus_tag="IXF88_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508397.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            11671..12174
                     /locus_tag="IXF88_001665"
     CDS             11671..12174
                     /locus_tag="IXF88_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1508398.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            12301..13338
                     /locus_tag="IXF88_001666"
     CDS             12301..13338
                     /locus_tag="IXF88_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508399.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            13353..>13974
                     /locus_tag="IXF88_001667"
     CDS             13353..>13974
                     /locus_tag="IXF88_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAQ1508400.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKD"
BASE COUNT         4683 a         2289 c         3099 g         3903 t
ORIGIN      
        1 tattttgagt ttggctgttc aatcaattgt aggagcaatt gctcctacga ttgcttttgc
       61 tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac atcaatatgc
      121 tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc cgcattatgc
      181 agtgtacaac ggcgttaaac aagatatttt ctgtgttgaa ccaggagtcc cgatttacaa
      241 tgagttcact cctggatatg agaagaaccc gttaccagat atgtccgata aagccaaatt
      301 agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg tggcacagaa
      361 gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat cagatggtag
      421 tgccgtaaat atcgcagcca ttgaggcaaa aatcaatcaa gccattgccg attatcagaa
      481 gaaaccaagc ttccataata gtacggctaa aacagtatta gggcaatcga ctactgtgac
      541 ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaata ccgcaaacat
      601 tgattatcgt gtgaatggca atcagttgat aatcagccca aatgccaatt caaaagaaag
      661 tggtgtgttg acgcttaaga aatctgctgg taccggaaca cctgtcgcct ataaaaaggc
      721 tggccaacag actttaatgg ccggggcgat cgataagccg aacacttata cggtcaaaat
      781 tgatgtggaa atggagggtt ctttgaagat taaaaaagtt gataaagaat cgggtgatat
      841 tataccagga acggttttcc atttagactt tggaaaaact ttacccgcaa aagacgtgac
      901 caccgataaa gaaggcattg cgaccttaga tgagattcct catggtacaa aggtaaccat
      961 tactgaaaaa tcggtgccgg cgccttatac gattgatacc acacccatga ctgccacaat
     1021 taaagcaggt gaaaccattt cagtgacttc aaaaaatacg cgagaaaaag gtcaaattat
     1081 tctggataaa accggaattg aaacagggag tgatctttgg aacgataatt actctttagc
     1141 gggcaatacg tttgccattc gcaaggatag cccaactggt gaaatcgtcc aagaaatgac
     1201 cacggatgaa aaaggtcatg cagaaacacc aaaagagatt gccaatgcgt tggaattggg
     1261 aacctactat gtgactgaaa ccaaggctag tcatggcttt gtgaatactt tcaaaccagt
     1321 aaaagtcgaa ttgaattatg tcaatcaaac agtggctctt gttaccagta acgtaaaagg
     1381 gcaaaaccaa gaaattactg gtgaaaccac tttgacaaaa gaagacaaag ataccggtca
     1441 tgaaactcaa gggaaagctg agtttaaaag agctgaatac accttgttta ctgttaagga
     1501 tggaaaagcg gtcaaatgga ccgagaactt taaaccagaa ttgttgaagg gaacgaaagc
     1561 ttctgatgaa accgtaactt tgactttaga tgaaaagaat caagttgccg ttaaacactt
     1621 agcgattaat gagtattact ggcaagaaac caaagcgcct gaaggatata ccttggatga
     1681 aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg ctgtgattac
     1741 tcgagatgtt gcggcaaaag aacaagtgat tcgctttggc tttgatttct ttaaatttgc
     1801 tggatcaact actggcactg ccgaaactgg cttcaatgat ctaaccttta aagtgtcgcc
     1861 attggaagga accaatgaaa tcaccggtgc cgaagatgaa gcgaccacag cttataacga
     1921 gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg attatttact
     1981 tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa tccgttctac
     2041 ttttaaagaa aataaagagg attataccaa gagtgattat atctttacta ttaccgaaaa
     2101 agggcaaaag caaccgatta aaacggtaac ggtcccttat gggaatctaa ccaatgagaa
     2161 tttctcagtc agtttgaacc gtttgatgct ttatgatttg cctgaggaag aagatagctt
     2221 aacttctctt gcgacttgga aagaaggaga taaagaattg actactcttg attctaccga
     2281 gctagtcgat aaattgagct ataatctaca tgaaatcaaa gaagactggt atatcgtagc
     2341 tcaagccatc gatgtagatg ctacaaaagc tgcccaagaa aaagataaaa aagccaaacc
     2401 ggtggcgatt gcggaaacaa ccgcaacttt agcgaataaa gagaaaacag gaacttggga
     2461 gattctgcat aaattaactg tcgaacaagt tttagacaag accattgtct tattcaacta
     2521 tgtgtatgaa agcaaagaag cctatgaagc tggtgatgag ccggtagcga aggatgctag
     2581 cttgaacaat caagcgcaaa ctgttaattg tatggtggaa cgtcatgttt ccatccaaac
     2641 caaagcgcac ttagaagaag gttctcagac ctttactcat ggtgacgtgg tagatatgtt
     2701 tgatgatgta tccattaccc atgatgtact agatggctca aaagaagctt ttgaaacaat
     2761 tctgtacgct ttatttccag atggtacaag taaagaaatt tggaaatctg gcaagattga
     2821 ttacgaagtg aatgacaaag aatttaccaa aaccgtactt gcggaaaaag tggatacaag
     2881 taaatatcca gaaggtacaa gttttacctt caaagaaatt aactatgaca aagatgggaa
     2941 tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaactttaa cgcccaaaaa
     3001 agttccgaca acaccaagta caccagaaca atctgaaatg ccaactgttc caagtggctc
     3061 tcaagaatct agtcctacag tgaaggcatt ccctcaaacc ggggagaaaa cttccaatgt
     3121 gctactgttc attggcttta gcttgatctt tgcgacggct ggctattatt tttggaatcg
     3181 tcggaactaa ggtgataaga tgaaacatgt aaatagtaga aaacaatacg cgcgaccgcc
     3241 tcctaaaaaa gtggtcgcgc tatttttgta ttcaaaacta acaatgaaaa gaggaaaaaa
     3301 tatggaattg aaatttgttg taccagatat ggtcgagacg ttcggaaaac taacatacgg
     3361 tggtgaaggt gaagttctat cggaagggta tggtcgaaat actacaattg ttggtcgaag
     3421 ttaccatctg ttttcaaata aacaacgggc cgatgatatt gaagtggtgg tagctgcgga
     3481 agccggcgaa aaggattttg accaagatca accactaaaa gctgtaaatc cccacttaat
     3541 tgccaaaggc tatgaaattg agaatcgtgg gtttaccgat tacgtgttgt atgttgatga
     3601 tttagtgaaa gcataggagg aaaagaagat gagattaaca gaaggtattg tcgtagattc
     3661 aggattaacc tttggcaagt tgcgtttttc cgcattacgt cgagaagtac gcaagcaaaa
     3721 tgaggacgga acggttagta acgaggtaaa agaacggacc tataatttga aatcctccgc
     3781 gcaaggtcga atgatccaag tcagtattcc agcaaatgta cccttacgcg agtttgctta
     3841 tgatgcagag gtagagctgg tcaatccaat cgtggatacg gtcgccaatt atgtttttcg
     3901 ggaaggaacc accgttaatt ggtttatcaa ggcagatgat ttagtcttga aacgacaact
     3961 gaatcaagga aaacctacga accaaaatga aggaaagaaa taggtgaaga atatggaaat
     4021 taatgttgaa catattttag attgtctcga tcaatttggc aaaggtgagc taacggagga
     4081 acaactcact aacgcattaa cctatgatga aaaaatgttt ctgattatgc atcaagggtt
     4141 actggaagta ggtaacgatg ccaaagaaga ctttgatgta ctggattggt taggggaaga
     4201 agagtctttc tttatggttg tcgaggtaaa tgaaagttta tgtcggcagg cagaatctgt
     4261 tttggaagaa atcgatgtgg agatgccgga tgccatcgaa agatttttaa aacaattagt
     4321 ggaaacgaaa caacttcccg tagtagtaaa tgactagcat aactttttga aattgcccaa
     4381 tgaaatccta ttgggctttt tcgactggag gtggaaacca tcaaaatgta caaaggacat
     4441 cgcatacgcg caggagatca acacttgatt tatcactttg ttcttggctg gctaagtgcc
     4501 ttattcatcg gttggatggg cgtcttttat tttccagagc ttagacaatt tggtctttct
     4561 aaggtatcat tttccactat tgaaatcgct tggtccatga aagatttagt atgtctgctt
     4621 ggaagtctcg ctctttcagg aagtatgatg ttgctttata ttcatttttt tcctgatcat
     4681 tggcgaagtt tgtggaatcg acaaaagcta gccagaatga ttctagaaaa ccattggtac
     4741 gaagtgaagc aaactcaaag tgatggcttt ttcaaagatt tgaataggag tcgaaccaaa
     4801 gagactatca gttacttccc caaagtttat tatcgaatga aagatggctt actgtctatt
     4861 cgcgttcaaa tttcactggg aaaatatcaa gatcagctat tgaagttgga aaagaagtta
     4921 gaaagcggac tgtattgtga gttagtagaa aaggaactca aagactccta tgtggaatac
     4981 accttgttat acgatatgat tgccaatcga attgggatag aagaagtagt ggcagaaaat
     5041 gggacgttgc gattgatgaa aaatcaagta tgggcgtatg attccttgcc tcatatgtta
     5101 atcgctggtg gcacaggtgg tgggaagacc tatttcttac tcaccattat tcaagcacta
     5161 ttgaagtcag atgcagaatt gtttatcctc gatcccaaaa atgcggacct cgccgattta
     5221 ggtacggtaa tgcctcatgt ctattctcaa aaagaagaaa tttctaaatg tgtggaagat
     5281 ttttacgaac gcatgatggc tcgtagtagg tcaatgaaag aaatgcccaa ctacaaaaca
     5341 ggagagaatt atgcgtatct cggacttccg cccaactttt taatctttga tgaatacgtg
     5401 gcttatatgg gtgcgaatcg ttttcctaca agcatcgaat agatgtgaaa tggaagccta
     5461 ttgagattat caatagtgac aactaatgtt ttgagagatg gaatgaaagc cttcaagttg
     5521 agctgaaaca tccccgctaa tactcctaca tgctctaatc aatgaagtag attggttttg
     5581 gtatgaagct aggtgaagtc gtctgaaatc tgcctaagtg cttgagcata aggctgacta
     5641 tagggcgggc ggctgaaaaa gataactatg gtaagaatgt ctgaaaatga ctgacgaatc
     5701 tgcgaatgta cgggtctaaa aggctgatct tattgaaaga taggaacata cggacgtatg
     5761 gtgagatacc gacaagtaag gttccatgat atgaaaatcg ggatagctaa cgaagaagct
     5821 ataatttcac aggctcatag tagataccta aggttataca tgatagctaa aaacattgga
     5881 acgatgtaag cagaaaacta tcaattaaat gactacttct agataggtgc atataagtaa
     5941 taacgaaatt gctttgtttc tgtgaaagta agggcacagt accgatgaag cgtctaacaa
     6001 atgtggaggg atagccctaa gtctatttaa taacaatgaa tgttgaaaca gatgaagttc
     6061 aaggatctgg taagaacgtg ggaaaatccg ttatggaggt gccacgcttg aacacaaaaa
     6121 tacgttactg ggaatattat gatctacaag aaacattcga tcagctattt actcaaagta
     6181 gaaacgggaa aaagttttat caattatatg agttaatcat ttctgaaaat aatattcttt
     6241 tagcataccg tactatcaag gcaaacaagg ggtcttcgac accaggaacg gattccttca
     6301 caattgataa ctataaggaa atgaaccaag cagagtttat ccatcttatt ctcagtcatt
     6361 tagaaaatta caaaccaaaa tccataaagc gagtaatgat acctaaaccc aatggtgaga
     6421 aacgcccatt aggaattcca tgcatgattg atcgaatgat ccaacagatg ttcaagcaga
     6481 tactggagcc aatatgtgaa gctaagtttt atgaacatag ctacggtttc agaccattga
     6541 gaagtgcaaa gcatgctcta ggtcgcatta tgtatctcat aaacatcagt aaaatgcact
     6601 atgcagtaga cattgatatc aaaggtttct ttgataatgt aaaccaccgt ttactgataa
     6661 aacaattatg gaatatagga atttgtgaca aacaagtctt agctattctt tcaaaatctc
     6721 tgaaatcacc cattcaagga gaaggtatcc cgagcaaagg aacgatccaa ggaggtatca
     6781 tttctccact attatctaat gttgttctaa acgacttaga ccattgggtc tcaaagcagt
     6841 ggcatacctt tgaaacaaag tatccctata ctaaaggtta caataaattt cgtgctctaa
     6901 gagatacaaa tctaaagcaa ggctatattg tcaggtacgc agatgacttt aaaatcatga
     6961 ctaatgatta tccgacagca ttaaaatggt tccacgctgt caagctttac ctaaaagaca
     7021 gacttaaatt agatatttcc aatgagaagt ctaaaatcgt caatttgcgt aaatgtaagt
     7081 cggaattttt aggattcgct atttgcgtga aacaaaaagg gaaaaaatgg gtttgtaatt
     7141 cacgtatttc taacaaaaaa aaggatcaga ttaaagaaga gattaaacaa agaatcaaag
     7201 atattcaaaa gagtcctaca gcacaaaatg ctctcctgtt taatgctctc gtgctcggtg
     7261 ttcataatta tttcaggtat gccactcata ttaatttaga tttgaatagg atgaactacg
     7321 ttctttcatt cactacttac aaccgattat caaattgtag tacaagatgt taccccaaca
     7381 aagcttcccc aacttacaaa aagttttact caacaacgat gaaaacgtat aaagtagctg
     7441 gagtctattt gtaccctctt tgcgacgtat ctacaaaaac aatttacgga tttaatacgg
     7501 aggatacacc gtttactccg tttggtcgtc aaagattgga gcacaaaagc ctacagtctc
     7561 ttgtgttcca agaattaaga aaattgatgg aatcgaaaat accgaataga acggttgagt
     7621 atttagatag tagaatatct cgttactcaa tgaaatcagg caagtgtgag attacaaaac
     7681 aatttttgcc agcaaaagcc gttcactgtc atcactattt gccaaaaagt cttggtggag
     7741 acgacaaatt cgataacttg agaattattc ataaggatat ccatcttctg atccatacaa
     7801 caaataaaat gataatcgat cactacgtgc atgaactgaa actggtgcca gagcaaatag
     7861 caaaaatcaa cctttatcga aagatgtgta atctgcaaaa cattcaatag aaaataaata
     7921 gatggaacgc cgtatgaggt gaaagtctca cgtacggtgt ggagcggggg aaaagatgga
     7981 gattatatca aattcttacc tatcgctata gatgttaacg acaaaagaaa gtgcggtgat
     8041 tttgaataag cttaaacaaa tcgtgatgtt aggccgtcaa tctggtttct ttctgattct
     8101 agcttgtcaa aggccggatg cgaaatatct aggagatggg attcgcgatc agtttaactt
     8161 ccgtgtggct ttgggtcgta tgagtgagct tgggtattcc atgatgtttg gagaagtcga
     8221 taaaaatttt tttatgaaac gcatcaaagg acgaggttat gtagatacag ggggcagtgt
     8281 gattagtgag ttttatacgc cacttgtacc aaaagggtat gatttcttaa gggaaattag
     8341 caacattgtt ggattaaccg ttcatacgga aagagaaaat aatagtgtgt agcctaggaa
     8401 tatcaataat cctgggcttt tttattgagg tgatttatga catttttaga tttattcgcc
     8461 gggattggtg gctttcgctt aggtatggaa caagccggtc accagtgtat cggtttttgt
     8521 gaaattgatg aatttgctcg gaggagttat aaagcaatcc atgatacaag gaaggaggtg
     8581 gaaatgcatg acatcacaag tgtatcagac gaatttgttc gatccctcgg accagtggac
     8641 atcctttgcg gcggctttcc gtgccaagct ttttcaattg cgggaaagcg gcaaggattt
     8701 tccgatactc gaggtacttt attctttgaa atcgctcggt tcgccactct tctccaacct
     8761 aagtttctat tccttgagaa cgtccgggga ttgctcaatc acgaaggagg ggctacgttc
     8821 gagacgatcc tccgaacgtt ggatggattg gggtatgatg tggaatggca agtgcttaac
     8881 tcaaaggcct acgttcccca aagccgtgag cggatcttcc ttatcggaca ttctcgagat
     8941 gcatgtaccg aacaagtatt tcctatcatt ggatcgtctt caacatctga tcaaaacatc
     9001 agaaacttgg taaatatcaa tccttccaat cgaggaatgg gtggccaagt ctatggttca
     9061 gatagcgtag caccaacctt gaccggtgat gaaggaatta aaattgcctt accggtaaag
     9121 gatgggattt ctgtagcggg tatgttgtcg ggaaattttg aacaaggcaa taggatttat
     9181 gaggtaaccg gaacagcccc cactttgtca acgaaacaag ggggaacgaa gattatgatt
     9241 cggaaaaatg aaacagaata tcaagaggtg aagccagggg acagtgtgaa cctggctttt
     9301 cctaattcca caagtcgccg aggtcgtctg ggaaaacaaa gtgtgcatac cttactaaca
     9361 ggagaccaac aagcggtagt cacggatcag taccagattc gaaagttaac acctagggag
     9421 tgctggcggc ttcagggctt tccagattgg gcttttgatc gagcttctca agtcaattca
     9481 gatagtcagt tgtataaaca ggccggaaac tcagtaacag ttccggtcat ttttgatatt
     9541 gctagaagat taaagggggt aaaagcatat gatttatgac gagttgattg gagaaatcta
     9601 ctgggtgatt ggcaagattc aggcagatcc gaaattggaa gaagaactgt accggctcaa
     9661 ttttgatatt cgcaaaaacg gggtgaaagt ccaggtgatt cgtatgtagt ggatgaggaa
     9721 acggatgctc gaatggagct caatcaagtc atcggtgaat tggaacgaat tgcggacctt
     9781 gccaaagaac ctgatatccg ccaatacttg tttgagataa aagcagagtt ggaaattgag
     9841 ggtatcaccg cggagtaata gggaaataac ccactgatag gcactgaccg cgggggattt
     9901 gcgaagcaga aatcctccgt ggtgctgtgc ctgttcatgg acggcggcag ccggccatga
     9961 tgtctcaccc cccgtatcta acaggggggt acaaaaacac aaaaaacaag caagtaacca
    10021 taaaaaatgc ttgttgtatc aaggatatta agtaatttgt ataggtgtta actcatttgg
    10081 aaaagttaac accttgaatt tggaggtgaa aacttggcgc aacgaaattt agattatcgg
    10141 ttattaaaag aacgacgcaa cgaatatggc gtttcacaaa ataaattagc tactgcttgc
    10201 ggacttagcc gcccatacct aaatcagata gaaaacggtg gcgtaatcgc ctctacaaag
    10261 accatgagaa aaatttttga tcaactggaa agtttcaatc ccgatttacc tttaacgtta
    10321 ctatttgact atgtaaggat tcgctttccc acaacggatg tgcggaaaat tattcaagaa
    10381 attctccatt tgaaattcga gtatatgctt catgaagatt acgcctttta ctcctatcaa
    10441 gaacaatatg tcatgggcga tattgtagtg atgttatctc atgaagaaga taaaggtgtc
    10501 cttttggaat taaaaggtcg tggttgccgg cagtttgaaa cttttttact cgcccaaaag
    10561 cgtagctggt acgacttttt tgaagattgt ctaaaagccg gtggcgtgat gaaacgcttg
    10621 gatttagcaa tcaatgatcg agtggggcta ctggatattc ctgatttaac gaaaaaatgt
    10681 cagaaggaag aatgtatctc cttgtttcgt accttcaaaa gttaccgttc cggggaactg
    10741 ttgaaagctg atgaaaagga tggtatgggc aataccttat atattggcag cctcaaaagc
    10801 gaggtttatt tttgtttgta cgaaaaagat tatgaacaat atatcaagtt agggattcca
    10861 ttggaccaga ccgaaacgaa aaatcgtttt gagattcggt tgaaaaatga ccgtgcttat
    10921 tatgcgattc aagatttgtt aaaaggtcgt agcattgaaa gtaccacgtt ttccattatc
    10981 aatcggtatt tgcgatttgc ggataaagta gatggaaaac ggcgaaccaa ttggcccttg
    11041 aatgaacaat ggggtcgttt tattggacgg aatcggaaag agattcaatt aacttctgaa
    11101 ccgaaaccgt ataccattga acgaacatta aattggctag gtagacaagt cgcccctacg
    11161 tggaaaatgg ctaaggagtt ggatcgattg aatcaaacaa cgtatatcca ggatatggta
    11221 cagaatgcgc gactctcgga ccggcataaa aagattttgg agcaacaaag tatggcaatc
    11281 gaaaacttga ttgtatgaaa agaggaattt tgatggaaat tgtaaaagat atgttattgg
    11341 tacttggtgg cagtatgctt ggtgttaccg tgatgtgctt gatgaatgca agtgcagcag
    11401 cggattgagc aatggaaaag aaaggaaagt gacaaaatga atttcggaca aaatttatat
    11461 caatggtttt taaccaatgc tcaatcttta gtcttactag cgattgtggt cattggtttg
    11521 tttttaggat ttaaacggga gttttccaaa ctgattggct tcttggtgat tgctttgatt
    11581 gcggtaggtc tggtctttaa tgcttctggt gtgaaggatg tcctactcaa tttgtttaac
    11641 cgaattgtcg gtgcctgagg gggaatcaca atggaacaga tgcgtgttta tattgcgaac
    11701 cttggaaaat acaatgaagg tgagttggtg ggggcatggt ttaccccacc tgtcgatttt
    11761 gatgaagtga aagaacagat tggattaaat gacgaatatg aagagtacgc gattcatgat
    11821 tatgaactgc cttttgagat tgatgagtat acgccaattg aagagattaa tcggttgtgt
    11881 aacttagcgg aggaactaga aggaacgcca attggtgaag ctgcttctga gattcaacac
    11941 gctttcttca attctttcga ggaaatggta gagcatgtgg atgatattgt ctattatcca
    12001 gattgcgatg atatgagtga tgtggcgcat tacttgattg tagaagctgg tgattttggc
    12061 gaagttccag aacaattgaa gccgtatatt gattttgatg cttgcggtcg agatttggag
    12121 attggaggaa attatttggt tactagtcgt ggagtttttg agtatccaga atgaatcaaa
    12181 cactatatat agtgtttgat tagtatatgt aagcaaaaaa acacaatata tagagtgcaa
    12241 actgaagggt aatgtgatat aatatttgcc gaagaaatat ttttgaaatg aggtaatatt
    12301 atggcaaata ttcaaacaag ttttattgat ttccataatt caattagatt ggacgtagaa
    12361 gacaatacgc tacttaagga ctataaagac caagtaattg atggattaaa agattactta
    12421 cctgatgatg tgaagtttga aactttttta cagggaagct attctgttta taccggaata
    12481 aaaagctgtg atgaaaaaat tgattttgac atcgatattg cagttgcttt cgaaattgat
    12541 catactgttt atgaagatcc aagggagcct aaactgtggg taaaagaggc gctagttgaa
    12601 atttttccta atgctcaagt taatttaaaa gtaccttgtg taactgctac atttactgga
    12661 aaaaagacaa aaaaaaatgt gcacgttgat gttgcggttt atgctaaaga agatgaaaat
    12721 tatttcttag caaaagcaaa agaattcagt gctcccgaaa accgatgctg ggaggaagcg
    12781 gatcccaaag tattaaaaga gaaaattaat agtcatgtag cagattcaga tgatcgaaaa
    12841 caatttcgaa gatgtattcg ctatttaaaa agatggaaag ataacaattt taaccaagaa
    12901 tataaaccaa caggaattgg acttacaatt aatgtgatgg atacgttcct agttaataag
    12961 tccactgatt ttttaactag aaaagttcag tataatgata tggaatgtat gaagcagatt
    13021 gtttcttcgc tgaaagattc atttgtgtat gagtacagtg aaacagatgg ttggcattat
    13081 cgtctacatg catatcttcc agtgaagcct aacagtgaca cctattcaaa aatgacagtg
    13141 aatcagatga gtgattttaa aaacaaactt tctaaattat atgatgattt gattttcgct
    13201 atcgatactg aagatgagta tgaagctaca aaacgattaa ataatcaatt tggggaggat
    13261 tttttaatta tttcagaaga agaagttaca gaaaaaaact taagaaatgc atttgttaca
    13321 gactatccga gtgcttaggt tggtgttaaa gattggatta tactgatatt ttaaaagaaa
    13381 agctaacaga tgttgtgcgt ttattcgatg gtaattacta tggggatttt tcatttgcta
    13441 caattggaaa gttgcgttta aaaattgaaa taccaaaaca ttttccattg gtacttccta
    13501 ggatttacat tcataatttt gatcatttag gactttacct accacatgta gaaaaagatg
    13561 ggttggtatg tttttcttca gatataaatt atttagtaga tgaagaaaat tcagaattgg
    13621 ttttattgca gtgtgttaat aaaactataa aaacaattga ggaaggtttg aaagggttaa
    13681 ataaaaatga ttttcgaaaa gagtttgttg ctttttggga caaacaagag aatcaaattt
    13741 cgttattcta cctttgtgaa ccagcgttgt ttatcaagtc agttgataca ataatgtacg
    13801 gtaagtcttt ggttgctttg gataaagaga acgaattaca attaaatgca gcgagtagat
    13861 tgtttaatgg agagaaagaa gatactcatg aattagatgc atactatgtt ccaattagaa
    13921 acaagaataa tattatgccc ccgaagaata ataaagcatg gtcaaagaag gata
//
LOCUS       DACESX010000066        13969 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.066, whole genome shotgun sequence.
ACCESSION   DACESX010000066 DACESX010000000
VERSION     DACESX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.066"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(325..2322)
                     /gene="tkt"
                     /locus_tag="IXF88_001668"
     CDS             complement(325..2322)
                     /gene="tkt"
                     /locus_tag="IXF88_001668"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1508401.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2424..2675)
                     /locus_tag="IXF88_001669"
     CDS             complement(2424..2675)
                     /locus_tag="IXF88_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1508402.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2822..3445
                     /gene="lexA"
                     /locus_tag="IXF88_001670"
     CDS             2822..3445
                     /gene="lexA"
                     /locus_tag="IXF88_001670"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1508403.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3624..3842
                     /locus_tag="IXF88_001671"
     CDS             3624..3842
                     /locus_tag="IXF88_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508404.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4130..4642)
                     /locus_tag="IXF88_001672"
     CDS             complement(4130..4642)
                     /locus_tag="IXF88_001672"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1508405.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="IXF88_001673"
     CDS             complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="IXF88_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1508406.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7095..7529)
                     /locus_tag="IXF88_001674"
     CDS             complement(7095..7529)
                     /locus_tag="IXF88_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1508407.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7539..8327)
                     /locus_tag="IXF88_001675"
     CDS             complement(7539..8327)
                     /locus_tag="IXF88_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1508408.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8324..9265)
                     /locus_tag="IXF88_001676"
     CDS             complement(8324..9265)
                     /locus_tag="IXF88_001676"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1508409.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9442..10029)
                     /locus_tag="IXF88_001677"
     CDS             complement(9442..10029)
                     /locus_tag="IXF88_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1508410.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="IXF88_001678"
     CDS             complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="IXF88_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1508411.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11851..12861
                     /gene="gap"
                     /locus_tag="IXF88_001679"
     CDS             11851..12861
                     /gene="gap"
                     /locus_tag="IXF88_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1508412.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12946..13881)
                     /locus_tag="IXF88_001680"
     CDS             complement(12946..13881)
                     /locus_tag="IXF88_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1508413.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3856 a         3130 c         2455 g         4528 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat
      781 agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc
      841 tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg
      901 ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac
      961 aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa
     1021 gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc
     1081 cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa
     1141 atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca
     1201 gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc
     1261 ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc
     1321 atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa
     1381 catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg
     1441 ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc
     1501 catcttgatg ccatcttcac caataggagc accatgaaca gcagatgttc cttctttagg
     1561 agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt
     1621 tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg
     1681 ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg
     1741 accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg
     1801 acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat
     1861 tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg
     1921 cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt
     1981 ggcttctaca ccatctgtgt gatgtacttc aggatgtcct ggtgttttag aatcccattg
     2041 acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata
     2101 caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt
     2161 tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc
     2221 gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt
     2281 acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa
     2341 tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa
     2401 gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc
     2461 ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc
     2521 attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgtaag
     2581 ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt
     2641 tcattgatcc tctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt
     2701 catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc
     2761 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
     2821 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
     2881 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
     2941 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
     3001 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
     3061 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
     3121 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
     3181 tatgctgaca attcgtggag atagtatgat caatgctggg atcttggatg gtgacaacgt
     3241 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
     3301 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
     3361 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
     3421 actttatcgt agtcatatct attaattgct actaataaaa gaaaactttt taaaaagagt
     3481 ttgagacgat ggtctcaaac tcttttttcg cccgggttct tcttctttca aatttttatc
     3541 acttccctct ttacaaaccg aacaaatatt cgtatactaa atatacgaac aattgttcgc
     3601 ataaattaac gaggtgagaa ttcatgaaat caatgagacg tcttggctta gtattaactg
     3661 ttttgttttt aggtattatt attggtaatt tcggcatacg tccagcttta cttgtgtttg
     3721 ttccgttatt tactttgtgg ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc
     3781 ggcgcagaat tcatgaagat catttctatc atgagcccta ctacagatcc aggcaatcat
     3841 aataaggatt gactgctaat tattcaaaag acaagaagtt aaacttttac gggggtgtga
     3901 caaaaatcgg tcacatcctt ttttcgaata aacggtgttc taaagccagt ttttcggtat
     3961 taagtcaaat tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat
     4021 ttataaaaaa atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt
     4081 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
     4141 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
     4201 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
     4261 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
     4321 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
     4381 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
     4441 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
     4501 gtttttcttt tgcataattt acgatttgct tcgtagcttc ccgataagcg tcaccatctg
     4561 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
     4621 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
     4681 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgccgctgg
     4741 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
     4801 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
     4861 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
     4921 tgagaatgga tcagtttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
     4981 taggcctcat caggcgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
     5041 acttctttca gatccgctaa atcatctgga caatccataa taactagaga gttcggtttt
     5101 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     5161 ataatctcag gcacgaattt caacgtagaa gaatcaaatg ctaaataaag gctaccttcc
     5221 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     5281 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     5341 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     5401 aaagcgaccg catcaagctg actcgatgca ttggaaagga caaatttcag atgctgttgg
     5461 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     5521 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     5581 tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     5641 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     5701 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     5761 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     5821 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     5881 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     5941 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     6001 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     6061 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     6121 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     6181 tgaagtccta tccgttctga atgatgaatg gcattgattc ctaatgaaac aaggattcga
     6241 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     6301 tctacaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     6361 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     6421 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     6481 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     6541 ccttcttcga tcttttcttg gtatacagat tggtttggtc catacccatg ctcaaatcga
     6601 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     6661 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     6721 acagcttctt ggattcggct gattgcttta tccatatcgt gcatcagata cggatcatgt
     6781 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     6841 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     6901 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     6961 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     7021 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     7081 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     7141 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     7201 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     7261 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     7321 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     7381 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     7441 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     7501 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     7561 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     7621 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     7681 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     7741 gccttatcga agaaatctgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     7801 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     7861 gaataaaccg ttgattttgc agttgccatt tttccagcca tagacgcgat attaatgata
     7921 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     7981 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     8041 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     8101 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     8161 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     8221 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     8281 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     8341 cgcgaaatgg aatctcaatg ctatcaaaat ctttcatgat cgtcgtgttt tcaaacactt
     8401 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     8461 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     8521 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     8581 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     8641 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     8701 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     8761 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     8821 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     8881 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     8941 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     9001 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     9061 tatgatcgcc gtgtaaatgt gtaataaaaa ttttaccgat ttttctcgga cgaatagtcg
     9121 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     9181 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     9241 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     9301 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     9361 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     9421 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     9481 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     9541 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgggtaa tttctccgcc
     9601 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     9661 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     9721 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     9781 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     9841 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     9901 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     9961 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
    10021 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgta
    10081 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
    10141 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct
    10201 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
    10261 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
    10321 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
    10381 gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
    10441 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
    10501 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
    10561 ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
    10621 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
    10681 gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
    10741 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
    10801 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
    10861 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
    10921 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttagggacga
    10981 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
    11041 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
    11101 gttcgatttt tcgctcttgt cctggttcgc cgttttcagc aatttccggt gcaggattcc
    11161 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
    11221 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
    11281 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
    11341 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
    11401 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
    11461 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
    11521 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
    11581 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
    11641 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
    11701 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
    11761 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
    11821 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
    11881 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
    11941 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
    12001 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
    12061 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac
    12121 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
    12181 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
    12241 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
    12301 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
    12361 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
    12421 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
    12481 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
    12541 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg
    12601 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
    12661 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
    12721 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
    12781 aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta
    12841 gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat
    12901 ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg
    12961 gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc
    13021 atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt
    13081 atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg
    13141 ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac
    13201 ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc
    13261 tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt
    13321 cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac
    13381 accgtataga acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg
    13441 tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg
    13501 tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt
    13561 ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt
    13621 cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca
    13681 atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg
    13741 tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt
    13801 ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag
    13861 gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg
    13921 aattagtata atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DACESX010000067        13875 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.067, whole genome shotgun sequence.
ACCESSION   DACESX010000067 DACESX010000000
VERSION     DACESX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.067"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            98..946
                     /locus_tag="IXF88_001681"
     CDS             98..946
                     /locus_tag="IXF88_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508414.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1117..1458)
                     /locus_tag="IXF88_001682"
     CDS             complement(1117..1458)
                     /locus_tag="IXF88_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1508415.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1544..2251
                     /locus_tag="IXF88_001683"
     CDS             1544..2251
                     /locus_tag="IXF88_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1508416.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2238..2561
                     /locus_tag="IXF88_001684"
     CDS             2238..2561
                     /locus_tag="IXF88_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1508417.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2650..3687)
                     /locus_tag="IXF88_001685"
     CDS             complement(2650..3687)
                     /locus_tag="IXF88_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1508418.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4134..5117)
                     /locus_tag="IXF88_001686"
     CDS             complement(4134..5117)
                     /locus_tag="IXF88_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1508419.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5533..6303)
                     /locus_tag="IXF88_001687"
     CDS             complement(5533..6303)
                     /locus_tag="IXF88_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508420.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6296..7231)
                     /locus_tag="IXF88_001688"
     CDS             complement(6296..7231)
                     /locus_tag="IXF88_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508421.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7825..9474)
                     /locus_tag="IXF88_001689"
     CDS             complement(7825..9474)
                     /locus_tag="IXF88_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1508422.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9669..10565)
                     /locus_tag="IXF88_001690"
     CDS             complement(9669..10565)
                     /locus_tag="IXF88_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1508423.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10558..11238)
                     /locus_tag="IXF88_001691"
     CDS             complement(10558..11238)
                     /locus_tag="IXF88_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1508424.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11239..12021)
                     /locus_tag="IXF88_001692"
     CDS             complement(11239..12021)
                     /locus_tag="IXF88_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1508425.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12014..12931)
                     /locus_tag="IXF88_001693"
     CDS             complement(12014..12931)
                     /locus_tag="IXF88_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1508426.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(12934..13218)
                     /locus_tag="IXF88_001694"
     CDS             complement(12934..13218)
                     /locus_tag="IXF88_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1508427.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT         4089 a         2972 c         2438 g         4376 t
ORIGIN      
        1 agcaaaagtg attatcaaat atgtaaaatc agctactttt ttcttaaacg tttctttata
       61 ctaaatataa ataagtgttt catgaaagga gaactagatg aataattggc ttgagtattt
      121 tcctgaaaac gttttggaac gcggttacag ctatcatctg cacggttttg tccgtcattt
      181 gaactataca agcaagtact tgtccgcaac agtttctggc acggaagatt ataaggtagt
      241 gatcacttgg gatgaaaaga ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa
      301 aaaatgcaaa cacatggctg ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc
      361 caactactca ctttccgaac tctcgtccct tgtttcctcc gctagttcct ctcttgtacg
      421 ggagctttta acagagattc ttatagaaca tccgcacttt atcgaacgct tcaaagttaa
      481 gatgccattt catgcaataa attattcaga taaattaaca actatcatcc ataaatacga
      541 tcatattatc aagaaaaaca aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa
      601 gttcatccaa gaagcagttg aatcgcttat tcagcaaaat gcctatcttc ccgcattcga
      661 attgatcaat gaagtgattg ctactctcga aacattctat tgggagcctg aggatgaacg
      721 aacccttctt ctgatagagg actgttatta tttatggaaa gaattattag cagaagcacc
      781 tcatgcagaa aaaaggcaaa tgttctcttg gttcgtctgc caagtcgatc atactgacgc
      841 ctcttattca aaacgctact ctataaaaat cctgaaagaa gactttcggg aaaaagaatt
      901 ttctaaccaa aagaaaaaaa tagataaaaa actaaagaaa cggtaaagaa agacgatttc
      961 aatgaaaaat agcaagtaaa cgataacaac catcctaaat ccacaagaaa tacagcagac
     1021 cttgctttaa aaacacgaat catgcaaagg atgaagtaaa gaagacttaa cgcgtaccca
     1081 atattagtca tttgactatt aggtacgcgt tttgttttat aagtcatgac aaatttcctt
     1141 cgttgctgga tagattttcc ggcattgttg atagatttta tgatactttt ctacattttg
     1201 taagagaggt tccgctgcat ctgtatattg gataaaattc tgacaacaac tttcaggtgt
     1261 gtcgaaccac cctaacccta gtgcagcaat cattgttgcg cctaatgcag gtccttcctc
     1321 cacacttaaa caatagaccg ttgcatcgaa aatatcagct tggatttgta gccatgcaag
     1381 atttttcgcg cctcctccca ccgaaacaat acgtgtaaat ctgcgacccg ctagttcttt
     1441 acgtttcaat cccaccattg acaaatgctc attaaaaatt tagaatattc acaagtttaa
     1501 cgacaattat gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga
     1561 tataaaaact gcgatcaaag acacgttacc taccgttttc ggttatatcg gtattggact
     1621 tgcatttggt atcgttggga aagctgccgg atttcatcca ctagtcgtca cgttgatgtc
     1681 cctgctggtc tatgctggtt ctgcccaatt tatcacagtc agcatgcttg ctagtcacag
     1741 cccattgctt tccatcgttt tctcgacctt tctagtcaat tcccgaatga ttctgatgag
     1801 tatgacgatt gctccttatt tcaagaaaaa tcggctgctt caaaatctgc tgattggcac
     1861 attgctgaca gatgaaagtt tcgcactggg aatgaataaa ttgaactata ctggacaaaa
     1921 gttaaacttt cgttggatga atacagctaa ttggatttct tatttgacgt gggtgggttc
     1981 ttctcttgtt ggcgcacttt tagggaattt tattactgat ccgaaaaagt ttggtttgga
     2041 tttcgccatc gtcgctatgt ttatcgggtt actttatctt caagtcattt ctgatagaaa
     2101 cacctctaaa cgattacagt tgatcctgat tggtctcact ttgattctgg tatatgtcgg
     2161 actgattttt atcccaagca acttagtgat tgtagttgta acactgattg ggtgcggatt
     2221 gggggtgtgg atcaaacatg ccttcttttg agtttacatt gctgactatt ttaggttgta
     2281 ctttagcaac ttggctttct agggtcctac catttgtctt actgaaaaaa ttcgatttac
     2341 cgcaaccgtt gcttgagtat ttaagttttg tcccgatcgt cattatgtct gctttatggt
     2401 tcagcagtct atttacccaa aatatcggac atctgccgca gatcaatctc gaaaatgctt
     2461 tggcttcagt tcctacactt ttagctgcta ttctttcaaa aagcttattg gtgatcgtct
     2521 tggcaggaat tctttccctt tcgttgattc gcttattata aataaaaaaa caagagctta
     2581 tggcaaatta ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg
     2641 tattcttgct tatccgttga acggaccact aacaacgact tttcctaaca tatgtcctgt
     2701 ttctacgtct ttggttgctt gtttcaagtt cttcgcattc atcccatctg aatagacctt
     2761 gctcaatgta ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc
     2821 tccttgcgtt tcgatttcat agttatagtc tgttttagcg aacatatact cccagtcgaa
     2881 gctgatagat tggtttttcc aaacacctat atctaacggc tctttgatcc ctacgatcgt
     2941 tcccacatgt ccaaatggtc ggacaaggtg cttgatttga tcaaagtagc gggtaatatc
     3001 gaataataca gcgacatact ctacttgatc gattcctaat tcacgaaaac ttttacttaa
     3061 gtcgtgatga taatcgattg gatagtctac acctgttttt ttcagccatt caaaattctt
     3121 cggacttgca gtcgcgtaga cttctaggcc agcccaatgc gctagttgat tcaaaatcga
     3181 accgacgccc ccggatccgt tgatcactaa gatttttttc ccgcgattgg cattttcttc
     3241 tggagttaga ccaaattttt caaataacaa ttcataagcg gtcaatgagg tcaacggcaa
     3301 ggctgcagct tcttcatctg aaagattttt tggtgcaaga gcgacaatac gttcgtcaac
     3361 tagttggtat tcttgattgc ttcctgctct tgttgttgta cctgcgtaaa aaacgcgatc
     3421 gccgacagaa aatttttgca cttcgtcacc tacagcaaca actttcccga ctccatcaaa
     3481 acctaagata cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg tatcgacagg
     3541 attaacagat actgcgttga cttttaccag aagatcccgt gaactgggta caggtatctg
     3601 ttctttttca tcaataaagc tttttggatc atcgatcggc aacccttcat aaaaaccgac
     3661 taccttcatt aattcctttt ctgacatacg aattcctcct tagcttcatc aagttggagc
     3721 atccatacag tgttcatttc aatatatata gtatgctatt tgttccacct tttttattca
     3781 tcataattat tctcttactt aaagtaaact atttgcactt ttttggttaa aaaagaggtt
     3841 gcgacaagac ttttgtcaca acctcttata aacggtgttc tcctaccaac tcttcggtca
     3901 caagtcaaaa tattcatcaa acatgagaag ctgttttttg aaattgctcc ttgtgactca
     3961 gagttaaacg gcaggctcac aacctctgat ataaacggtg ttcaatcaac tgaccctcgg
     4021 tattagctca aaaaacgaca aaatatgaag agctattttt gtttggctgt tcttattact
     4081 tgatacagga cagctttttc acgacctcta attcacggtg ttcaagcaag aaatcattgt
     4141 tcactgaaaa gcgttggatg ttctttttca aaggtctcta agatctctcg tacttcatca
     4201 gtagatttcg tattcatcaa ctgaactcgc aagtcatttg ctcctggaaa tcctttgata
     4261 tagattttaa agaaacgatg tagtcctgtg atggaacgcg ggatgatttc tgaataatga
     4321 tcttgcagat ctagttgcag acgaagtagt ccgatcagtt ctttttctgt atgttccctc
     4381 gggtcttttt caaatgcata aggattcttg aaaatccctc ggccaatcat gatgccatca
     4441 acgccatatt tctcggccaa ttcttctcct gtctggcgat cggggatatc tccattgatc
     4501 gtgattagtg tttgtggagc gatttggtca cgtaaagcaa tgattttagg aatgacttcc
     4561 caatgtgcat ctactttact catctcttta cgcgtacgca aatggacaga aagattggct
     4621 atatcttgat gaagtaggtg actgatccat tcttccatct cagacatttc cgtatagccg
     4681 atacgcgtct tgacgctgac aggcaaaccg ccagctttag ctgcttcaat caattgtgcc
     4741 gctacttctg gacgaaggat caagccactg ccttttccac gttctgctac attagggact
     4801 gggcagccca tgttgatatc gattccttgg aagcccattt ctgccatagc aatgctcatt
     4861 tctctaaaaa attctggctt gtctccccaa atatgggcaa ccatcggctg ttcatcttct
     4921 gtaaatgtta atcttcctcg tacactgtct tttccatctg gatgacagaa actgtctgag
     4981 tttgtaaact cagtaaagaa gacatccggt gctgctgcat gctttacgac atggcgaaat
     5041 acaacatctg ttacatcttc cattggtgct aaaacaaaaa aaggctttgg cagctctttc
     5101 caaaaattag tagtcattta tttgttactc tccattctat caagcagtga agtcgcaatc
     5161 tttctcaaaa acattgtcat tatactagga aacaaaaaaa gaagcaaagt gaacagctta
     5221 aaagatgttt ctgattgagc aaaaaaaaga ctgtgacaaa aatcttatca cagtccattt
     5281 tccggaataa atggtgttca aaagcttgca tttgcggtaa taagtccaac caaacgaaaa
     5341 ataggaggag ctatttttgt ttggctgttc ttattacttg atgcagaaca gctttttcac
     5401 aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattcgctca aaaaacaaca
     5461 aaatatgagg aactattttc ccgtttgttt ctctaatact caggtcaaaa cgctgatttc
     5521 acaacctctt ttctaacttc tcaatcctct tcctttattg atcaaatacc aagtaacggc
     5581 gtacaaaaca acgatgaata acacaagtac gatcatcgat gttgctactg gcacatcgat
     5641 cgttccaagg aatccgtaac ggaaaccgga gatcatataa acgatcggat tgacttttga
     5701 aacagcttgc caaaagggcg gtaacataga gatggcatag aaaacgccac ctagataagt
     5761 taacggctgc aagacaaatg taggcacgat cgatacatca tcataagact gagcaaaaat
     5821 cccgttgagc agtcctgcta atgaaaacaa gattgccgtc atcaataatg taatgatgac
     5881 catgaaccat gaatacacat gaagtggtac aaagaacaag gaaatgatcg ttacgagaga
     5941 gccgacgaga atactgcgac ccacaccacc aatcacaaag ccccaaataa tcacatgcgt
     6001 aggaacaggt gctaccaaga tttcttcgat atttttctga aatttttgag agaagaagga
     6061 cgaagaaaca ttggcatacg aactagtgat agcagacatc ataatcaaac caggaacgat
     6121 gaattccata tatgaaaaac cacccatgtc accgatccgc ccaccgatca tttttccgaa
     6181 aataacaaaa tacaaggatg tcgtgatcac tggcggaacc agtgtttgga cccatatacg
     6241 caaatagcgg ttcgtttcct tgatagctaa actttttaat gcagtaaaat aaagattaaa
     6301 catttttctc ctccgtatga ttattttctt ctgtgatttt caagaacagt tcttctaaac
     6361 gattcgactt attcctcata gaaagaactt tgattccttg tcttgtaagc tgatcgaaga
     6421 ttccgttcac cccttgattt cgttcaactt cgacagcgat tgtctgctca tcttcaaatg
     6481 tacaagcata tccttcaatc acaggtttgg tatcatatgg cgctaaatca aagataaatg
     6541 tttcaaattg cagttttgac agaagctctt tcatacttgt attctcgatc agttcacccg
     6601 attggatgat tccgatgttg cgacaaagca tttcagcttc ttctagataa tgcgtcgtca
     6661 aaatgatcgt tgtaccattt tcgttcaatt ctcgtagaaa ctcccacatc tcgcgtctca
     6721 actcgatgtc aactcctgct gttggttcat ccaagatcag caaacgtggt tcatgcatca
     6781 atgccctagc aatcatcagt cttcgcttca ttcccccgga aagcatacgt gcacggacat
     6841 ttctcttttc ccaaagattg gactgcttca aatatttttc gctgcgtttc atcgcttctt
     6901 tgcgagagac accgtagtag cctgcttgat tcacgacgat ttgctggacc gtttcaaaag
     6961 ggttaaaatt gaactcttgt ggaaccaatc caatctgttg ctttgctcga actagatcct
     7021 tgtccagatc ataaccgaag acattcactt ttccagatgt tttattgact aaagaagtaa
     7081 taatcccgat cgttgtagac ttcccagctc catttggccc taatagcgca tagaaatctc
     7141 cttctttcac cgaaagatcg attccccgca atgcttcgac cccacttcca tagatctttt
     7201 tcaaattatt tatttcaagc gcatttgcca tttatttcct atctccttat agacatgatt
     7261 cttaattgta tcatggaata cagcagatga aaaaacaact tggcttccac acaaaaacac
     7321 acctgctaac aaagaggaga tgtgttttct agttcatgat catcttcgat agaataacta
     7381 tctaaacaac tctggaactt ttaaaatcat aaaagtaccg acagcaacta ctacgatgac
     7441 atatagaaga tccttataac tgaggttctt gtctttttta ttcatgttaa gctttcctcc
     7501 gtccttttat cgaaagcttt tttcttttat tttaaccgaa ataaataaaa tggaaatagt
     7561 tattttcaat ttctgttttc gctgtttgtt ttacggagaa ctttgccatg gaacgtgcgt
     7621 ctcgtcgctg atgctaagtt ttttcgataa aagctgctag aaggaagaca gcgtggttta
     7681 ttttgtatcg tcttttgcta aatcttccaa gatcatctgg acagcagact cgttcccatt
     7741 gaattctgcc acttttttgg catcaatgta cttcttttga taatcgtctt tttggaccca
     7801 cgcatcatgc ttcgtttgat aaagttaatc aaacggatac cactgacgct gttcatcgaa
     7861 caacaaactt tctttttggg cctgttcaac tatggattta ggataattca aaactttcag
     7921 caaggcaatc gcatttcggg tccttgaagg tccttctttt aatttgaaat caaaactgat
     7981 tccttttcct tctgttactt gttcttcaaa gtggacattc tcacaataat tctttaaaat
     8041 ctctgtcaat tcgatatcat gtgtggcgac aaatgctaaa gatggatact ccgcaagcca
     8101 ccgaacgact gaagcggaag aagcaatccg ttcgatcgta tttgttcctt ttaaaatctc
     8161 atctacgaag cagtagcagc gttctttgga agccacttgg tccagcaaac gtttgatcga
     8221 tttgatttct gaaacaaaat aactgtcgcc ttcaaataaa tcatcttcga ttgccatgga
     8281 agtcaacaca tgtccaggtt gcagggtaaa ctgttctgca atcgctgttt ggatcgtctg
     8341 tgctagaata cagctgatgg ctatactttt gacatacgta gattttcctg atgcattgga
     8401 acccgtgact aatgtattct tctgccagtt gactggatta actaccgctt cttttaacaa
     8461 tggatgatag atatctttcg ctgtcacacc gccctcagta aactctggtc ggcaagtgat
     8521 cggcatatag gtgcgataat tcaaaacggc tgctgcaact tccaattctc ccagcaattc
     8581 ccacagcttg atggcttcag ttgaatgctt ttctaattta gtgagaacga aatgatagga
     8641 gataaatggc agcatgaaca tgatgttgac atattcaaaa atcaattctg cttcactccc
     8701 gctcttcatt cgaaatgaaa aacctaattt agggatgtct cttaatggtt tagtttgctg
     8761 tttcagctct ttttgaagtg gtgtcgtcat ttttgctaac tgattggcgg ctgaaatcgt
     8821 tgaaaccaaa taactcatgc tattcagctc tgtttctagt gtttgtttct tcactagata
     8881 atagaccgta ttgaatacta agccaccgat tgctagatag attcctagta cttgccctaa
     8941 aagaagcaaa cctacaccca ccagcggaag caagccaaga aaaataaata aacctacgcg
     9001 tccaatcttt ttactttttc catccgctag atattgtttt gcaaaattat gatctttttt
     9061 tcctaaacga gcgaattgat attggattct ttctctcagt tgaggatttt cctgataaaa
     9121 agcaattagt ttttctaact gataatcttc tccaaaatca aagctacgta aacgctgata
     9181 caacgcttct gaacctacac tagaataggt actgttgatc ccttcaaata cttcgaacat
     9241 atccaagtca taccaagtaa gatcgtcgat ttcactatcc catgaacgga attttttctc
     9301 cgtgagccac gcatccttca aactttcttc tttatcaaat ctcggttgat aaggaatttt
     9361 tccccaatta ctacgaactg tttcctttac tttcaaacgt gaaaaaatgt ctaaaacaac
     9421 taataaaata acaactgcaa taataattaa aacgataaat tctctttggc ccatatccct
     9481 ctccgttcct tgatttgtta gttttagtat agcactgttg cgctatttga aaagggggta
     9541 caaaaataac tgtcttcgtt acctaattcg tatcaatcac accaaaataa aaacaccttc
     9601 caaaaaagaa gatgtcttta aaacgagata caattttttg tcattcactt caggtaaatt
     9661 agagccaatt aaaccctccg caacttgacg gagatggaga agatgccccc gtctaatcta
     9721 gcggtcatct ctcctcccat tccttccacc aatttttttg acaggtaaag cccgattccg
     9781 ctagattccg tatttgtgcg gctggtattt tcggtataaa aacgctggaa gactttttca
     9841 acagaggttt gcgctcctgg tttgatagga ttggccactg ttaatcgaat gaatgaatcc
     9901 tcttttgaaa atgtcacctt tgcttgtcca tctgcataac gcaataaatt ctgtatgata
     9961 ttttgcagca ctcgcagcaa catgagcgga tctgctaata cagttgtatt tatcgagtgt
    10021 tcagcgaacg ctacctcgat atttttctca gaaaattgtt cataaaaaga gaaaaatact
    10081 tcttccacct ctttagaaaa agaaagcgta tggagatcca tttctttttg cttcgtctct
    10141 attcgagcaa gatcataaaa ctgatccgtc atactgagta atcttgcaac agattgtttg
    10201 atgatcgtta aatacttctc ccgtttctcc gtatcctcta cctgttccag taattgtaca
    10261 taaccgttaa tagcagtcaa aggagttctg agatcatgag aaaggccgac gatcgatagt
    10321 ctgatgtttt cttccatttc ttgaaatgct tgtccttttt gttcattttc atccacccat
    10381 gtattcagtg catctactag gtccattaga ttcttttctc gaaattcgac tgaaagacga
    10441 ccaccatatt cttgtcgttc agaaagagtg gcgattttct tagttaatct cttgatttcc
    10501 ctttgcagga agaaaataaa acttcccata aagataacaa cgagaagcaa cagccactta
    10561 atcaaatcta aaccccactc cccacaccgt atgaatatac acagcatgtg cctttgctag
    10621 tttccctcga agattggaaa tatgcatatt cactgttttc tcactatcaa aatatggttc
    10681 ttgccaaact gtttcataaa gatttgctcg cgaaaatact tttttcggat gttctaaaaa
    10741 aagcttcagc agatcgaatt ctcttcctgt caaatggaca gccacaccat tcacatttac
    10801 atccctcgac tctttgtcca gtaagatttc cttgtaatag atgcgttgcg acagatcttt
    10861 tacttggaca gaagtctgat gacgaagttg gatgccgatt cgtaacaaca gttcttcgat
    10921 atcaaatggt ttcgtcacat agtcatccgc tcctgctgcc aacaaggaaa ctttatctct
    10981 ttgatcgctt tttgctgaaa gaacgatgat cggaacattc gatgtctgtc ggatttctgc
    11041 taacaatgct tcacctgaaa gccctggaag catgagatct agaaggatca aatcgacagg
    11101 atatgtgctt aacaataatt ttccttccgt acctgaataa gcatcaagaa gtttgtatct
    11161 ctgttcaaga acctcctcca gtaaagaatg aacagcttca tcgtcttcga taatcaaaat
    11221 agtttccagc aggaacacct acttaatgtc agatttttgg aaaataaaca ttcctagagc
    11281 tgatgatacc acgattgtta ctagactgac cagaatcatt ttcggtgctt gcgaataagt
    11341 tgcttgttgc gcaaagtccg tatacatgag ccacgtttcc gtaaaccact ccgttagaaa
    11401 ttccaagcct ttgatttttg cgaaaacacc gggaacgaag gccgttccca tgatataaag
    11461 aaaagaaaga agcaaactga cactggtact tctagtgatg aatgtcaaag ccactggaac
    11521 agataatgtc gctaatatca gcagaagcaa tacaggaaat acttttagaa cataatttcc
    11581 atagtttttg attccctcaa cttcaccaaa tccgaaagca agtgtgtaag caatcatggc
    11641 tagccccgta atcaaaacca cgccaagtgt tgtgatcaaa agcgtaacga gaagtttcga
    11701 cacataataa gtcattcgat tccgtcctaa agataacgta ttgcgaatcg taccgttttg
    11761 aaattcttcc ccaatataaa agcacgcaaa tgctgcgaaa gccagaagaa tccatgcagc
    11821 cattttttcc accattaaaa tccctgttgc accgttgatg actgtatccg ttgcttggtt
    11881 taattcattg ccgtattttg tatgcatata ccattcgttc caagaataaa aaagcggaag
    11941 tacgaccatc agtcctagtg agacccacat tttacggctc ttccatactt ttattttttc
    12001 agcagttaat agattaaaca tgtggattgc ctccagttaa ttgtaagaaa taatcttcca
    12061 gtttttgccc aactaggtgg ataccatcca caggaacttt tgcctctacc aatgcttgat
    12121 tgatggctgc tacttggtca attccttcaa aaagctggat aacacggcta gattggacaa
    12181 aataatccct ataaccaaga gagtcgagaa ccgttactgc ttgttccgtc gccgaagtgt
    12241 caatttttat aaattgacga gattcttgtg ccaattcttc tttcgataat tctttgatca
    12301 agtgtccttc atgcaaaata ccgtagtgag tggcgatctg tgacaattca tctagcaagt
    12361 ggctagaaag caagacggtg atgccccgtt cttttaccaa acgttggatg atttctcgca
    12421 tttctgccat ccctgctgga tccatcccat tcgtcggttc atccaaaatc aaaaattctg
    12481 gattcgtgat cagtgcattt gcaatagcta aacgctggcg catacctaaa gagaaattct
    12541 ttgctttctt tttccctgta ttttccaatc gcatcatttt cagcaggtct tcgatttctc
    12601 tatccgacac accgccgttc gctgcttgga tacgtaggtt atctcttgca gttaattctg
    12661 gataaagagc aggggtttca atcgattgac ccatacgtcg tcttgctgcc tgcaaatccg
    12721 tcgttccaaa taattcgatc gaaccttcat caatcgagat caaacccata atagttctca
    12781 taaaggtaga cttacccgct ccattctccc caatcaaacc atagatcatt ccacgtttga
    12841 tttcgatcga tactttatct aatgctttgg cagcaccgta ttttttggtt ataccagttg
    12901 ctttcaagac cgtttcattt ccaaaatcca tacttatcct tctttcttat tgataagtga
    12961 agtatagttg acgggtataa agaaagcagc cagaaatgta taaagaaagt gtaaattttc
    13021 caattgaatt tcaagaaaaa tcagactgtc tcgcagacgt ggttgagttg tctgccaatc
    13081 aaagagcgca ccaaacagtg ctaatccaag tggactggcc accatggtaa tcgttgttat
    13141 cgttgacatc agacgtcctt gtatatgcgt tggaacttct cgctgaagcc agaccatata
    13201 aaaagcaaaa gaaaacatta atccttaaaa acggacttat gaaaaataat acatgccgtt
    13261 tgggatttac aattagctac caaataagag gttatgacaa aagacttttc acaatccctt
    13321 cttccgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatttatcaa
    13381 acatgagaag caatttttgt aaattccttc ttatttcttg aagctgacca cttctgtcac
    13441 aacctcagaa gcggataaat aatattgttt gatgatactt tatctttttc ccgtttgttt
    13501 atagatcgaa tgatattata gaccaagaat cctacctatt aacatagcta aaaacacaaa
    13561 aaaggcactt tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt aactgcgctc
    13621 agcattgctt cgcccagtac gattctcgta actcgtgaac tcgaacgata atcgcaatta
    13681 acgttttgag aactgtgaag ctttacgggc tttcttaaga cctggttttt tcttcgtatc
    13741 attgaataag tttgaaaagc cttgatttat aaaggttttt ccgtaaatat attttgttat
    13801 atttgattaa atttgaaatg tttgtgttct aaaatgtgtt ctaatttaaa ggcagtatat
    13861 ttcgtatttt atatt
//
LOCUS       DACESX010000068        13722 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.068, whole genome shotgun sequence.
ACCESSION   DACESX010000068 DACESX010000000
VERSION     DACESX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.068"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            63..257
                     /locus_tag="IXF88_001695"
     CDS             63..257
                     /locus_tag="IXF88_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508428.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            526..825
                     /locus_tag="IXF88_001696"
     CDS             526..825
                     /locus_tag="IXF88_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508429.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(922..1356)
                     /locus_tag="IXF88_001697"
     CDS             complement(922..1356)
                     /locus_tag="IXF88_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1508430.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1588..2298)
                     /locus_tag="IXF88_001698"
     CDS             complement(1588..2298)
                     /locus_tag="IXF88_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1508431.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2288..3148)
                     /locus_tag="IXF88_001699"
     CDS             complement(2288..3148)
                     /locus_tag="IXF88_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1508432.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3153..3791)
                     /locus_tag="IXF88_001700"
     CDS             complement(3153..3791)
                     /locus_tag="IXF88_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1508433.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3806..4105)
                     /locus_tag="IXF88_001701"
     CDS             complement(3806..4105)
                     /locus_tag="IXF88_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1508434.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4148..5632)
                     /locus_tag="IXF88_001702"
     CDS             complement(4148..5632)
                     /locus_tag="IXF88_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1508435.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5652..6113)
                     /locus_tag="IXF88_001703"
     CDS             complement(5652..6113)
                     /locus_tag="IXF88_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1508436.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6131..7198)
                     /gene="ulaG"
                     /locus_tag="IXF88_001704"
     CDS             complement(6131..7198)
                     /gene="ulaG"
                     /locus_tag="IXF88_001704"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1508437.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7416..8186
                     /locus_tag="IXF88_001705"
     CDS             7416..8186
                     /locus_tag="IXF88_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1508438.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8459..9286)
                     /locus_tag="IXF88_001706"
     CDS             complement(8459..9286)
                     /locus_tag="IXF88_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1508439.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9854..11089
                     /locus_tag="IXF88_001707"
     CDS             9854..11089
                     /locus_tag="IXF88_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1508440.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12088..12348)
                     /locus_tag="IXF88_001708"
     CDS             complement(12088..12348)
                     /locus_tag="IXF88_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1508441.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVTHLNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12375..13676)
                     /locus_tag="IXF88_001709"
     CDS             complement(12375..13676)
                     /locus_tag="IXF88_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1508442.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4405 a         2820 c         2166 g         4331 t
ORIGIN      
        1 aatcattttc acttacatat atttgaatat taactaaaaa cagcaagaaa gaagaggatc
       61 aaatgtatta tgtagaagta aaaacaaaag gcgtcaaaaa caaacaatat gtgaaaggta
      121 tcagtaacga atatccttta cttggttcat ggaaagaagc tgctcctttt tccaaaccat
      181 gtgccataaa aataaagaac gagctagaaa aagaactgac ttgtggtaaa gctgttgtag
      241 atatcatcga aaagtaaatc caatgtatag aaaggaagat cacacatgaa aagcgacgta
      301 caaattgcta agattataga attagaagaa gaagacgcag tgttgacaat tgaaaaaaaa
      361 taaatgtttg tataccatat gattatttca atatgtatcc tggaattgga gatctcgtag
      421 agctatatca agatgaatca aatatacttg tcattcatca tgaaatatag agacttataa
      481 aattgtttat ttttataatg tatcagtata gaaaggaaat aaaacatgga ttatactgtt
      541 gaatttgaca gtgaactact taagcaattt ccacaagaag aacatttcat gtataaagaa
      601 gcaatttccc acaaagtgac agagtatttg gagcataatt tttttcgtat aaagccagtg
      661 cgtactcgtt cggcaaaagg aatatacgag atgaaagtca aaattaataa aaaaaattat
      721 cgcatagcat ttgctttaaa aaaaaatcat gtgttcattt tttatattag ttcgaatttg
      781 caaaaaattg tatttgataa agaggtgagt aaaaaaataa aatagctgag cattggagga
      841 tagattgagt gatactaagt ctatcctctt gtttttctaa agaactaagc tagaattttt
      901 tcttttgtac gttattatat gttatttgtc attaccaaaa agcacctttt ttcttctagt
      961 atctatgaca taagaaatac cacccatttc ctttacttga acaaatccta ctgattcata
     1021 aaattttttt gttttctcag tatcatctgt caataattcg atttggactg gtccataact
     1081 agtgatcttc tccaaagctt cattcaaaag cactttgcct attccttggc gctgatacct
     1141 cggatgaatg aggatatctt gaataaatgc gatcgtcgca aaatcagtga tccaacgaac
     1201 gattccgata atcttccctt ccccattacg ctttatcaga gattcagatt gttcaaatgc
     1261 tctagctaac cttgcagtgt cttttgtgta attcgtccaa ccaacgctct tatataattc
     1321 agaaatctca tcgatcataa gggaatcaat attcattttt ttcttccttt cttatacaat
     1381 tctccctcta ataatacagt tctttgacta tttaatctat cacatataac tctcctgtaa
     1441 agattctctc gattccttaa aattcctctt tccttttaga atgcatactt tttgataaat
     1501 aaaaaggttg tgataaattt tcttcacaac ctctgtttct ctctatcaag tgttatcttt
     1561 taattttcta cccttaccct agcatctcta ttgcccgtag tatgcatact ttccatgttt
     1621 gcgaaaataa tgtttatcta gtaaatattg cgggatttca catatattgg ggttgatcag
     1681 ctcgttttct ttggccatca gacaaatttc atctaagatc aaactgtttt ctactgcttt
     1741 cataggtgaa tccccccatg tgaacggtcc atgtccatag acaagtactc cagggacttc
     1801 gtttggatct aattttcttt ccttaaacgt ctcgacaatc acattccctg tatgaacttc
     1861 gtaagcttct cttacttctt ctttcgttaa ttggcgtgta caaggaacct taccataaaa
     1921 agcatcagca tgagtcgtgc cataagctgg caaatcgcgt cctgcttgcg cccacatgac
     1981 ggagtgcgtg gaatgagtat gagtaatagc agttatatct tcaaaagttt gatacaaaac
     2041 aacatgtgtg gggagatctg aagatggttt taagctatct tcttctatta tatttcccga
     2101 caaatctgtt accaccattt gatctgcttg catgcattca tatctgacac cgcttggctt
     2161 aatgacgatg actcctagct cacggtccac ttcactcaca ttgccccaag tcaatttgac
     2221 taatccgtat cgcggcaatg ccagatttgc ttcaaggaca cgttgcttca ttgctgcaat
     2281 tttttcttca caagacatca tagccagcct ccttcaattt agaacttaag tagtcttttg
     2341 cctgctggat ttctcttcga aagtcttggc ttttttcact ccacatttca atcagaaatg
     2401 ttccatcata atctagacgt ttcaaatgtc ttaataatcc aagaaaatca acacaaccgc
     2461 taccaaaagg aacatcacga aactggcctg aatattcttt tgttacaggg taagtatctt
     2521 ttaagtggat agcagtgata taatcgatcc ctttctcaag ttctcgagcc ggattatttt
     2581 ccggccatgc ggacaaattg cctaggtctg gatatacttg tagaaaaggt gaatgaatct
     2641 gttctttgat ttctaaaaat tttgaaatgg agttcataaa tggatcatcc atgatttcca
     2701 cggaaagaat gatgccatat ttagaagctt cctttacgct ttcttttatt ccttcgataa
     2761 agtcttctct ggaattcatt gatttctctt cataataaac atcgtatcca gctatttgga
     2821 ttactttgac gcttagatta tgcgccaaat ggattgcttt tttcattagt tgtttcgctg
     2881 cttttttctt ttctggatca gcagaaccaa acggaaatcg ccgatgcgca cttaaacaaa
     2941 tcgaatggat acgtacaccg gagctgaaca tctcttctct cagctcagct atcttttctt
     3001 cagaccaatc taatcgtgct aaccgctcat ccgtctcatc aatcgacatc tcgataaaat
     3061 cgaagtccat ttcttttaca agggaaaaac gttctttcca tgaaatatct ttgggtaacg
     3121 ctttttcata aatcccaatt tgagccattt ccttaccccc agatacgaat gatctcatct
     3181 ttgaagtttt gtgcggcctt catcggatca tttgctgctg taataccacg accagtgata
     3241 aaggtataca catcgatccc ttcaaataag tgtaatgtgt gtggatttaa tccaccagtt
     3301 acagaaacat taaagcctag ttcaatcagt tttttgatct tcgataaatc tttttcaccc
     3361 caagtttcac cagataacag cgcatcacgg ctttgatggt agattacctg ccggataccg
     3421 atgttatgcc aatccatggc ttgttcaaaa gtccaattgc cgtagagttc tacttgcaat
     3481 tcacctattt ctttttgtgc tgcctccata gttggaatcg ttgcacaaca aatgactgtc
     3541 atccagtctg ctcctgcttt tgcaacatta ctcgccactg ttcctccagc gtctgcacat
     3601 ttcgtatcag cgactagttt tttatctggg aacatactgc gaatacagcg aatcgctttt
     3661 tgtccttcct gtaagcaaag aattgtgccc acttcaataa tatctactac atcaccaact
     3721 ttcataacat ctcctaatgc atgttctaag tcattgtgat ctaacgcaat ttgcaaattc
     3781 ggcctgctca tcttagttcc ttcctttact tgtttttttg gtaccacttt gtttcagcaa
     3841 atttttcact gatttctttg actgacatca catttttgat accgataact gtaactcctt
     3901 ttttctctgc ctgagtaaac atattcaaaa aattgactgg cgtaaaaact acatcgtatt
     3961 ggccagctga actttttccc tctgaaatgg cacaatgatg gattttggta attggtacgt
     4021 ttagttcttt cattgctttt tccacacttc tcatcatcat caagcttgtt cctgatccat
     4081 ttgcacacga tactaaaatt ctcatcttaa tccctccatt atgagtgttg tctaatcaga
     4141 ttttctttta tactctctgc ttttctgcat atgtgtcata gtcttctaca atcaaaaaat
     4201 aaccatcagg atttttccga tattgtaact gcggaatagc caataaacca atgacaacta
     4261 cggcaattcc agcataacct aggactttca tgattactgt aaacaatggc caagctgttg
     4321 cccagtcgaa catcccgata taaccaccgt atgttgacat cccaatccat gtagcgaata
     4381 atgctgaacc gcctacttgt atgattcctg aaagaaacgg gaacaggcaa gcggctttga
     4441 atccacctcg gttattggca taaactgcta taacggcatt atcaaagaac aacggaatga
     4501 aaccggctat gacgatcgtt ggtgatttca ataaaatcag taaaccaatc atcaaaaact
     4561 gtcccaaagc accaaacaag aacccaatcg ttaccgcatt tggcgaacca aagccaaaag
     4621 tagcagctac gtcaattcct ggaacagctc ctggtaaaat cgtgtttgag attccttgga
     4681 aagattgagt tagttcatcg acgaatgtcc gaacacctaa ttgcaagatc gccagatata
     4741 ccgcaaaatt taaagaagtc gtcataatgt agaataagaa actctgccct tcttgcatga
     4801 attgcgcttg aatcaaataa tctttgccta gtacaatcag aatgatccca aagaaaaata
     4861 gcatgagtaa cgaagtagca accatattct cattgaaaat cgacaagaat cctggtaatt
     4921 tgatatcttc aatttttttg tcttgcttga ttgatctatt tttcttttca tcccgctttt
     4981 tcatccactc agctaatttt gcaaatataa agattccgaa catttgctga tgtgccactg
     5041 caaatccggc accttctgtc aattcctgcg tgattcctac tgttaagtta gaagctactg
     5101 cccaataaca acctaaaatc agtcccataa aaatcaatac ttgtatccta cctagatcag
     5161 gaaagcaaaa cagtaaaatc caaaaagcgg tagctgcttg ttgaacctga acgtttccgg
     5221 tagtgaaaac ggaacgcagt ttcgtatatt tctgaaaacg gactaataag atatttaaaa
     5281 caaatgcaat cagtaataaa atcatcacat ctccaaaggt acggccaaat gtctccatta
     5341 atccagcatc cacagcattt tgtccaaaat aaggatctgt aaccatggca tccaaattga
     5401 accgttcctt tagtccaacc aagattggcc gaaaattatt gaccaaacca ccagagccaa
     5461 cagtcaaaat aaagtaaccg accgttgcct ttaagaatcc agcgatgcat tcatgaagcg
     5521 ggcgtttcag caaaatatag cctaataaca cgataaaacc aatcaggtat gctggctgcg
     5581 tcaagatatt tgttgcaaaa tacgtccaaa tgcttaacaa aaattccccc atcttttttc
     5641 tcctctatca attaattata ttctgccatc acaacttggt aatcatcgat ggagttcgta
     5701 gagatgagac gctcaaccaa tccatctgtc attaataatt cggataattt ttggatattt
     5761 tctagatgtt cttctggatc ttttgctgcg agtgtaaaaa acaaactagc cgttttctcc
     5821 tcattctccc cttcactttc aaaacggatt ggctgtttca acttggtaaa tgaaatagct
     5881 gttccaaaaa caccatcact tttttctgaa gaatgcggca ttgctacttg aggtgcgatc
     5941 acaatataag gaccgtattt ttctacacaa tggatgatct cctccacata cgtttcatca
     6001 ataatttcct tatcgatcaa tacaagacag ctttcggcga tagcttcacg ccaatccgtg
     6061 atttctttat ctgggtaatg aatcaattga tggtcaaaaa agtatttcag caagaaactc
     6121 cccttctttc ttataaaaac gacggaaacg gtgtatcatt ttcaatcgag aagacatctt
     6181 caaatcctcg ataaaaatta aactcaagat catctttgtt tgtcggatag atgaatttcc
     6241 cgcctacttg ccagatataa ggtttaaatc catacttcag gcgatccttt ttgaatttcc
     6301 aaatttcagt gatctctttt ggatcagcca taaaattaga ccaaatatcg tagtggacag
     6361 gaatcactac ttttgcattc aatgactccg ccatgcgcag catatcaaca gaagtaactt
     6421 tgtctgttat ccctcgtggg ttttcaccat aggcccctaa acagacatcg attttgtgtt
     6481 cattaccatg tttggcaaat aaattggagt aatgtgaatc tcctgcatga taaagattcc
     6541 ctcctgatgt ttcgaataaa tagtttactg caatttgatc catctcctga ggcatttttc
     6601 cttttaatac ctcttctgga tcttcacaag tgaccaacgc tgtccgatca aacgcttcta
     6661 aagctactac attgatgtct ttgattttga ctgcatcacc tggctttact gtaatcgttt
     6721 tttcttcagg aattccccat ttttgccacg tgcgaaccac ttcttttggc ccaatgaatg
     6781 ttgcttcagg acaattttga tgaactgctg cggctgtatg gatatctaga tgatcggaat
     6841 ggatatgtgt aacgattaaa gcgtctacat tttttatcgc aaaaggatcg attacgaaag
     6901 gctgagtacg taaattaggc tgcatatttt gaacgccact catacgcatc atctgatggc
     6961 cttttttcat catgccattc ccatgactac gttttcccgt cccacaccat agatcacata
     7021 aaatattcgt tccttcatga cttttcagcc aaattcccgt acagcccagc caccacatag
     7081 caaaagttcc aggatccact acttctgttt cgatttcttc attcaagtat gtgccccact
     7141 caggaaatgt ttctactaac cacttttctt ttgttacttc attgacagtt ttactcattt
     7201 gcttgctccc cccatatcgt tgttccaact tacaagtaaa tattaatgtg tatcagcatc
     7261 catgaaaacc cttttatatg accgtttgtt ttttatatga ttttctttca gtaatcaatg
     7321 attgtttcag tataataaag acagatatga atgtttaaat gaacaactag tcaaataaat
     7381 ccatggatca agatgtccct caggaggcaa aagtcatgaa aaactcactt gtcaatattg
     7441 aaaaacgtca aaaagaaatt ttggaactgc ttcagcagac tgaacaacta tcaactttcg
     7501 aattagctga atcccttgat gtatctatta gtacgatacg aagagattta aatttattag
     7561 aagaaaaaaa cgagatcata cgcaattacg gttattgttc ttataattac aaaaaccaga
     7621 ctgattttga tcaatctggt cctgaaagga ttaaacaagc gattgccaga gaagctagta
     7681 aatacattaa cgattatgat acgctgttta tcaattctag ctcgactgcg ttgaaaacac
     7741 tggattttct tcaatctaac catttgacag tcattacaaa caacgtgaaa atcaattact
     7801 ctccgcacaa agataattgt agttatattt taactggtgg agagatgcgc tttccaaaag
     7861 aagcactagt gggcgacatc gcattaaata cgatttcttc agtcaatgca gacatttgta
     7921 tcattggctg tagcggagtc gatgtagtac atggggtcac aacaaagata ttaaatgaat
     7981 caacgatcaa cgaaatgatg gtcaacaaga caatcaaatg taagatttta gtcgctgatc
     8041 atcgaaaaat cggactgaca tcaaagttca agattgctga tatttcagtc tttgattatt
     8101 tgatcactga taaattttgt tctgaaaaag tattagagga gatcagaaat acggggataa
     8161 aaaccattca agtagattca ttttaagaac caggacgtaa atatgtaaat aggattatca
     8221 aaaaaatgaa taacaaattt atcgataaat gaaaaacacg ctacgctcaa attgataaag
     8281 gaatcccaag atgtatccat ccaatcgata ccatcataat ctattgcttc ttttataacc
     8341 gttatatgtt gcctaaatag gaatcaaaca atatcgaaaa aaggaggtat gacaaaagtc
     8401 acacctcctt ttttcgaata aacggtattc aaatcagaac actgatttca caaccacttt
     8461 atttcaggtc gttttcaatc acctttaccg cgtaatcatt tccgttgaaa cgcgctattt
     8521 tttttgcgtc catatatctt tctaaataat catcttctcc taattgctta tggcaagaag
     8581 caatttcata aagcaagtat tccaatgcat aagtcgattt atgtttctta ttatactcaa
     8641 tacccttctc acataattgg attgctttat cataatcctc gattttacgg taataggtgg
     8701 cgatatcata atatacaatg cagaccatca gattatcaaa atcagtctca ctcaatattt
     8761 taagtgtacg ctccacataa actttcgcaa aatctaattc tccttttgac aagtagatca
     8821 ttgccacgtt tgcagctgct tgtaagtgat ggatatctgt ttcggtcgtt tgttccaaca
     8881 cttgattaaa ataaaataat gctgcatctg gttttttcgc ataaagatga tcaagcagtc
     8941 cttgataaaa taaaatttga tttaaaagat cagaattact tactttttcc tgatcgatcg
     9001 tttgtaaaat tttataagat tcttggtatt ttgatacaga attcaatgat tgaatctcat
     9061 ttattttttt cctgattttg tcttcttctg tttctactaa tatatcggag atctcgacat
     9121 tcagcttttt gcaaagcgca cttaagatat tgatgcctgg cgacatattc ttgttttcga
     9181 tattgctgac agttgcttgt tcacaaatgc cttctgctaa ttgtacttgt gttaacccct
     9241 ttttctttcg gtattctttt agtaatgctc catctacttt aatcaaaaca atttccttcc
     9301 ctttaaaaaa ttatttctcc tttatatcat acacaaaaaa aagttctaaa caaccaaaaa
     9361 tgtttagaac agaataaaaa ttttaatttt aaaataatat tcaaattaaa caactggcgt
     9421 atctgctcct tgatttcttt ttgtgttatc tgaataatta tcgtttacag ttataacaca
     9481 tagtgaaaat tgtcatggcg agaaagtcta agaatatttt taaacataac tatttctttc
     9541 ttacgtgtgt ataatgacga cagcacacct atcaattaat ataattattt tattttttta
     9601 tgttctcatg tttattttta atgacacaac ttctcgtatt tatctggcac aacatataaa
     9661 tcgatcaaaa aattgttgcg tgtttttttg aaaattggag tttcaacttg attacaaaat
     9721 ttaaatttag aagttgtgaa aacagcgaga gacagtttaa tattcaaaat atacataggc
     9781 tactcgaacg ctgtgttttt tagtatacta gtaagaaacg aagggataga ctcattccaa
     9841 taaggaggat ttcatgaaga caattctttt tgctcctgca gtattcaatt tagctgagac
     9901 aacacgaatg attgaggttg ccaaagctat gaaagaccgt tattactgtg tattctttgg
     9961 cttttctaaa acttttgctc ctttgatcac agaaagtggc tttgagtatc atttactagc
    10021 gccgatcctt acgaaaaaac aagaagaaca aatcataaaa tttgaccagt tgcgtacact
    10081 taaaaaccct tttaccaaag aaatggttgc ctctcgtatc actagtgaac agagtctgat
    10141 tgctaagttt gagcctcgac tgattgtgac aggttcgaat gtgacaattt ttttatcggc
    10201 ccgcaataaa aagattcctt tagtatatgt taaaccctat gctttgagtg aacctttttt
    10261 tcaatcagag catgcgctga tgccccacga gttacgtttc ttcggccctt taagcaatcc
    10321 cctttggcac tggacaaaaa agcgtatagt aaatacccga tggatcccca atgccttcaa
    10381 acacgctgca caaaaagaaa tgcttccttt accaaaaaca agtttacaaa tgatggatgc
    10441 tgatctaaat ctcatcacaa cgcctaaact atttttggaa gaagaacagc tgccggaaaa
    10501 ttatcgaatc gttggtccaa tttttgctaa gctcagtact tccttgcctg aagaagtgct
    10561 aacttttata cagcgaaagc aacaagaaag aaaacgtatt atctatttcg caatgggtag
    10621 ttcaggcaat ccgcgtatgt tacgcaaaat tttgttcttc ttacaaaaac aaccggatct
    10681 agcggttatt gcacctattg actatctgct tgatgacatt caatctgact cagaagatct
    10741 tttaataaca gaatatctgt ctagtcatct tttgcatgaa ttcattgatt tttcttttat
    10801 ccacggcggg gaagggaccg tccaaactgc ctgtgaatcc ggcaaaccgt ttatcggtgt
    10861 ggggatgcaa tttgagcaaa gctgtaatat tgactattgt gtaaaatacg gaaacgcaat
    10921 cgccctcaca aaaccagtga ctgaaagaaa actgcagatt gctctgcgcg aagtttcttc
    10981 acccaagatt cgtcagcagg cacgcacgct gcaagccgtt tttccaataa atggtccaca
    11041 acaagcaatt caagaaatcg aaaccttcct gagcaaccac ccaatatagt ttcaagtaga
    11101 aaaagctttt ttaatagaga ctcatcagta atttcgctat tcgagaccca gacagtgaaa
    11161 atacaaaaaa agtaaatagt ggattgaaaa gttagagtta cttaaattac ggtgtctaaa
    11221 aaacacaaaa tcatccttaa aataaaagat ctgacgctat ccaaatgagc ataggtagcg
    11281 tctgccattt attcttagtc aaattctttg acacctttct ctggttggat catctcaacg
    11341 tttcttcaaa agaaagacca taataattag gcaaatacat tttcttaaaa tgtgtcatat
    11401 agatctagaa gcatcgcttt gtagagattt gtgccgctag tcccctcaac aattactaaa
    11461 tcaaatgtag gatttaccac tttatctttg ataatcaacc tccctcttct gttgagacaa
    11521 tgatctcgca atccgatgca tactcctata taaactgcca gcaatcccca catattgagt
    11581 agatcatgtc agaatgttct atgtcgtcag caacgataca ctaaaagtca cgttcaccat
    11641 gactgattgc tattcccagc acaattattt ctatacacgt ttcatgattt gaataaacac
    11701 tcacatcata catattacca ttcatacacc atactgcagc ccctactata tggttgtaag
    11761 ctcccaaatc tatatttgac ttgatcactt tggtagtaga tctactaatt ctaaatccat
    11821 tgctgataaa atttccattt tttcctcctt ctttctgcca aaacagttaa aacaataata
    11881 atctttatgt aaaaaagggc tgtgacaaaa gccttgtcac agtcccatat tccgaataaa
    11941 cggtggtcaa aagcttgcat ctgcggtaat aagttcaacc aaacgaaaaa taagccaaca
    12001 tttcacaaaa atttaagaag caattttcgt aaatttcttc ttatttcttg aagctaacca
    12061 cttctgttac aaccactttt agaacattta aagcttcgat gtgacaaacc atctatgctg
    12121 ctcgatcata aaattgatag caaaaattcc acaaagtaca tatgtccccc atgaaaacaa
    12181 aacattcaaa tgcgttacaa tgatctttgg aagaatcacg atcaaaaatg atgccagtgc
    12241 ccaaatccat cgattgattt gaatatgata tgtagctagc atattcaata taaggtgact
    12301 agccgttaat aaaaagataa aaacaaaaaa taaaatcagc caatacattg tttcccacct
    12361 tctaaaaacc aaacttagac agtctggact tcgttttctc cacgactttg ttcatctgac
    12421 agcatctttt tatcataagc ttttacaaat ggataataga taataaatgc tatcaatgtg
    12481 ttgatcatga tcaagataac aacacgccag tcatttcctg ctgaaagata accgccgatt
    12541 ggagctggta atgtccatgt tggcaaaaca gtaaatctat tgaccatttc taatttgact
    12601 gcaaaataac tgataatagt caaaactaaa ggaactaagt tgaacgggat gaacatgtaa
    12661 ggattcaaca tcataggagc tccaaaaatt atgggctcat tgatattgaa gataccagga
    12721 ataatagaaa aacgaccaac ctctttcaag aattttgatt tacagaagaa tataaacaaa
    12781 acagatacca cgatcgttgc tcctgcacca ccaattgtta ccgtccattg ataaaactgt
    12841 tcaggagcga tatagggtaa ttgggtggca gctgtaccat ctgccaatgc ctttgcattc
    12901 tcatccaaca tgatcagcca cattggacga acgacagccc ctacaatact catgccatgg
    12961 ataccaaatg accacaacaa atggatcaaa aacattggta gtaaaacacc aagcaaatta
    13021 ttccctaaaa tattggttaa aggctcgaat aacgataata aggcagcgtt tacatcaaag
    13081 cccgcgacgc ttcgaatcag ccaagtaatt gttaaaatga ctgcacctgg aatcaatgcc
    13141 tcaaaactac gggcaacagc aggtggcact tgttctggca ttttgatagt aatatttcga
    13201 tttttaaaga aacgatatac ttccactgca aaaatcatag ataagatccc cgcaaacatc
    13261 ccagatgctc ccaaagaact cataggtaag acccagccaa gaggtgcctc ttcaggagtt
    13321 aaaacgttta caggaacttg taacataaag aacgttccta aagataatat accacctgtc
    13381 acactatcta atccatatga tttagctaga tccgaaccca taacaaaagt tgcataaata
    13441 gccatcaatc ccatcgtcat cctaaagggt aaaacaatcg ttgccgaata aggggcaata
    13501 aattcattcc atccaggaaa cggtaaattg ataaagacca taaaaaatgt cccaatcaaa
    13561 atcaatgaca atgttgaaac aaccccatta cgaattgcta ataaatgacg ctgtgtacca
    13621 atttttgcca ttggtgggac aagtttctct tcgacccatt tcatcagctt ttccactaaa
    13681 accactcctt ttcaataaaa tatgaaccat tcattgcatg ac
//
LOCUS       DACESX010000069        13678 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.069, whole genome shotgun sequence.
ACCESSION   DACESX010000069 DACESX010000000
VERSION     DACESX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.069"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..901)
                     /locus_tag="IXF88_001710"
     CDS             complement(<1..901)
                     /locus_tag="IXF88_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1508443.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(925..969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1184..2152
                     /locus_tag="IXF88_001711"
     CDS             1184..2152
                     /locus_tag="IXF88_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1508444.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2265..2543
                     /locus_tag="IXF88_001712"
     CDS             2265..2543
                     /locus_tag="IXF88_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1508445.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2661..2897
                     /gene="secG"
                     /locus_tag="IXF88_001713"
     CDS             2661..2897
                     /gene="secG"
                     /locus_tag="IXF88_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1508446.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3009..3755
                     /locus_tag="IXF88_001714"
     CDS             3009..3755
                     /locus_tag="IXF88_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1508447.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3758..6115
                     /gene="rnr"
                     /locus_tag="IXF88_001715"
     CDS             3758..6115
                     /gene="rnr"
                     /locus_tag="IXF88_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1508448.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6137..6601
                     /gene="smpB"
                     /locus_tag="IXF88_001716"
     CDS             6137..6601
                     /gene="smpB"
                     /locus_tag="IXF88_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1508449.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6903..7622
                     /gene="atpB"
                     /locus_tag="IXF88_001717"
     CDS             6903..7622
                     /gene="atpB"
                     /locus_tag="IXF88_001717"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1508450.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7675..7890
                     /gene="atpE"
                     /locus_tag="IXF88_001718"
     CDS             7675..7890
                     /gene="atpE"
                     /locus_tag="IXF88_001718"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1508451.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7981..8505
                     /gene="atpF"
                     /locus_tag="IXF88_001719"
     CDS             7981..8505
                     /gene="atpF"
                     /locus_tag="IXF88_001719"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1508452.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8492..9034
                     /locus_tag="IXF88_001720"
     CDS             8492..9034
                     /locus_tag="IXF88_001720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1508453.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9057..10613
                     /locus_tag="IXF88_001721"
     CDS             9057..10613
                     /locus_tag="IXF88_001721"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1508454.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            10627..11529
                     /locus_tag="IXF88_001722"
     CDS             10627..11529
                     /locus_tag="IXF88_001722"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1508455.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11552..12958
                     /gene="atpD"
                     /locus_tag="IXF88_001723"
     CDS             11552..12958
                     /gene="atpD"
                     /locus_tag="IXF88_001723"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1508456.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12972..13394
                     /locus_tag="IXF88_001724"
     CDS             12972..13394
                     /locus_tag="IXF88_001724"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1508457.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4521 a         2447 c         2980 g         3730 t
ORIGIN      
        1 tttcggaaaa aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc
       61 aaaattcata acgatcaacc aatgtttgtc cacgacttat tccctgagtt tccacagcgg
      121 tgaagctttt gaaaccttca cagatggccc cctcgatgaa ataagcaaca gctaacggat
      181 cattgatgac acatcctaaa attctttctt gtttccagtg gaagtcaaaa taaaaacgag
      241 tgatcgcttt taaatactct ccctcttcag gattcatttg acagcagtat tcgaggatag
      301 tcggtgtcaa gacgatttct cttgtcacat ctaacccaac catttctatc gtttgtttga
      361 gattctcgaa tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg
      421 gcgaacagtt accatggctt ttgtaagttc cacccatcga gacaaaacga tccatatgtt
      481 tcccaatatt tgggttgact ttcaaagcaa aagcgatatt ggtcaaagga cccaaagcaa
      541 tgactgatgt atttgtcttt tctttgaaat aatcagccaa aaaatcaacg gcatggagag
      601 gttctgcctg ttttctcaag atcatcggga aatttgtttc ccctaaacca tccataccat
      661 gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt ttccattcct tgataaacag
      721 gaatgtctaa acgatcacag cgttccaggc atttcagcac attctgcgta cctatatctg
      781 cagggacatt cccacaaact acggtaatag caactacttc gatatcaggg gatttgacag
      841 ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca atcaataatt acttttcgca
      901 ttatttccaa tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac
      961 cagtccaaga cgagcgtctt gattataatg cacgaaatta gtatactggc ttttattgcg
     1021 aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa ctttaagtaa atgatacaat
     1081 gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat cctatggatc ggtacattgg
     1141 ataatagata gattatagtt tggcagaaag cggcgggtgt acaatggtta ttttttattt
     1201 cattcttatt ctgataatta tagggatcag ttaccatttt ctttcttatt tttatgcaaa
     1261 gaaattagta agagcgggag tacagagagg atatgactgg tatgaagaca ctggtcatcg
     1321 tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa cgacaaaaat tagaagaaga
     1381 gtgtgaaagc ttttgggagc agggaacacc caaaacaatc aaaagttatg acgggttgaa
     1441 actagctggt cagatgttca tccatccaaa taagcagaat aaatgggtca tttgcgtaca
     1501 tgattaccga agcacaggga aaagagacat gtctcatata ggtaaaagat atgcagagaa
     1561 aggattcaac gttcttgtac ctgatttacg tgcgcacgga gaaagtgaag gagaaatcat
     1621 cggaatggga tggctagatc ggctagattt aattgcctgg atccaactca tcttagatga
     1681 acagccgaat gcgtccatta ttttacacgg cggatcaatg ggagcttcta cgatcatgat
     1741 ggcaagtggt gaaaaacttc catcagcggt caaaggattt attttagaca gcggatatgt
     1801 ttctgtttat gcagaattcc gatatatgtt aagtaaaatc actgtctttc ctaaaaaaat
     1861 ggtcatgaga tatgcgaatc attatgccca gaaatacgcc ggttactcct taaagcaagc
     1921 atcagctacg agacagcttg gcagcaacca tctgccttta ttagtcatcc atggtgaaag
     1981 agatcatttt gtccctacag aagcagcgta cacgattcaa aatgctacgg caggggataa
     2041 agcactgctg cttgttccag aagcagaaca cttggaagca tcgatgaaag atccgaaaac
     2101 ctattggaca gtgatttttt catttattga acaaagaata acgatggatt aattttctct
     2161 tcttttttgt aagtaattcg aggataaata attgtttata gatctggaga ctgctaaaat
     2221 aaggtaggaa taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagaag
     2281 aaaaaaatcg tcttgcttta ttcaatgatg agcaacaaga gattggagaa atgacatggt
     2341 cagatgcggg cccagacatc atgatcattg accatacatt tgtagatcca gcatatcgtg
     2401 gtcaaaagtt agctgaaaag ctagtgtata ctggagtaga attagcaaga cgagacggga
     2461 aaaagatcat tcctttatgc ccttatgcaa aaaaagagtt tgaaaagaaa ccagaatatc
     2521 aagacgtctt acgtcagtca taagaataac agctgaaagt tttgaacttt cagctgttat
     2581 tccatttata tagagttgtc acggactttt atctatgata aagttaaaga gacgatatct
     2641 tttgggaagg aggctgcgat atgtataatt taattttggg aatcgtgatc gttttatcga
     2701 ttgttatggt cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat
     2761 ttaccggcgg tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca
     2821 tgcagcgatc aactgcagtt atgggagcta tctggatgat cttattattt gtcctagcat
     2881 atttatcttc gaagtagttg aataagcctc tcgcaaaacg gcgggcggct ttttttattg
     2941 caaagagaac gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg
     3001 tgagacgagt gagacaatta ccaaaacccc tttatgcaaa acatggaaag cgagcggttt
     3061 tgctgcttca cgcttactca ggaagtccaa acgatgttcg aatgctcgca cgatttttgg
     3121 aaaaagcaga ttatacagtt tatgcgccgc tttttaaagg acacggaaca atggagccat
     3181 atgatatctt gcaagaaaaa gcagaaagct ggtgggcaga cactaaaaaa gccatccatt
     3241 ttttacagtc tgagcagttt tctgatattg ccgtattagg attatcaatg ggcggcatct
     3301 ttgctgtacg agcactagaa gaagaatcag tgattggcgg cggttttttc tgttcgcctt
     3361 tgtctcctgt aaaaacaaac gtgcctgaaa actttgaaaa atacgtacgt caagtgctga
     3421 agacagcagg taaatcagag aaagaaatca atgaaaaagc agttgcttac cgctcgttag
     3481 ctgaacaaca attaatggat atacaagatc aggcagcaat cgtagaaagt aggttgtcag
     3541 atatccaaca gccgatattt ttagctcaag ctgggaaaga tgaaatgatc gaccctaatg
     3601 gcgtatttga aactgcacgc aagttgagcc gtcagcgagt gacacttcaa tggtatcctg
     3661 aaagtggtca tgtgattaca gttggtacag caagacgcga attagaaaaa gacgtcttgg
     3721 aatttttaga aaaattacct tggaatgagg aataaatatg acaaaagaaa cgataaaaga
     3781 gaaaattctc tttttcatgg aaagcagtcg caaacgcagt ttttctatgg aagaaatcgc
     3841 tgaaggttta gggttccaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca
     3901 aatggaaaga gaacagagta tagttttcaa taaaaaagga aaagtgaaac ttcctttaaa
     3961 accaatcttg atcgaaggaa catttcgagc aaatgaacga ggatttggat ttgtaacgat
     4021 tgatcccgaa gaagatgaca tctatatccc aaaagaagcg actggttttg caatggatgg
     4081 agacaccgta gcaatcgata tcatgaagcg agcaaataca gatatggatc gcggagcaga
     4141 ggggaaagta gtcgagattc gagaccgggc gactactcag ttagcagggg aattcgttgc
     4201 ttattcagaa gaagaaatac aagaaacaga tctttacggc gttgttatcc cgaaagatag
     4261 aaagctcaac caatttaaag tatatgcagc agcagaggga gttcgtccag ttgacggaag
     4321 tattgtgctg atcgaactga cccattaccc tgaaaaaggg tatgcgacta gtttagaagg
     4381 gataatcaag caagtgattg gtcataagaa tgaccctggc atggatatcc tctcaattgt
     4441 cgtggctaat ggtatcccta cgaaatttcc tgatgatgta ttgtctgaag cagatgaagt
     4501 tccagatcaa atcagcgaaa aggatataac tggacgtaga gatttaagaa atcagctcat
     4561 tgttacgatt gatggagaag atgcaaaaga tctagatgat gcagttacgg ttaaaaaatt
     4621 agataacggt aattattttc ttggagtaca cattgcagat gtttcttact atgtgacaca
     4681 aggcagtcaa ttggacagag aagcatatga aagagggaca agtgtatatt tgactgatcg
     4741 agtgattccg atgatcccgc aacgtttatc gaatggtatc tgttcactga atccccatgt
     4801 tccaaggctg accatgagtt gcgaaatgga gattgatcca aatggacaag tagtctctca
     4861 tgatattttc ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca
     4921 aatcttagaa gatcaagacg aagaaacgat gcaacgctat gctcagctcg tacctatgtt
     4981 ccaagaaatg ggtgaattgc atcgaatcct tgaaacgatg cgaatcaaac gtggcgcgat
     5041 ttcttttgag gatcgcgaag cgaaaatcct tgttgatgct gaaggacacc caaatgacat
     5101 ccagctaaga agcagaggaa ttggtgaacg cttgatcgaa tctttcatgc ttgcggccaa
     5161 tgaaacggta gctgaacatt ataataaatt aaatctgccg tttatttacc gaatccatga
     5221 acagccaaaa gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct
     5281 ggtccgagga acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt
     5341 agaagacaaa ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc
     5401 acgctattca gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt
     5461 tacatctcct attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag
     5521 tgaagataaa agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga
     5581 tcatagttct aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa
     5641 gaaagcagaa ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt
     5701 tgtgaaattc ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa
     5761 tgaattgaaa caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag
     5821 aactggtctg acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc
     5881 agatgaacga gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg
     5941 tccaaaagca cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc
     6001 agaaaaaaat ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa
     6061 aaaagggaaa aagccatttt acaaagatgt caaaaagaag aaaaagaaaa aataaaatga
     6121 gttggaggaa acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc
     6181 tcgccatgat tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga
     6241 gatcaagtcg atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa
     6301 cggggaagct ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa
     6361 tcatgatccg ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga
     6421 aaacgaattg aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg
     6481 ctatgccaag gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga
     6541 tttgaaacga aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata
     6601 atttcattat cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt
     6661 tagcatttaa tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt
     6721 gcatggggaa aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa
     6781 aatgttttga attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga
     6841 aaaaaccaga tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg
     6901 gattggatga acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt
     6961 gtataatggt gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa
     7021 atcttaagtt gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc
     7081 gaggaatcgt aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat
     7141 ttacattgtt tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct
     7201 tacctaacga aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt
     7261 tggcatttat aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag
     7321 gatatttcgt aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg
     7381 aagaatttac aaatttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg
     7441 aagttttgct aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat
     7501 tagcaattcc attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag
     7561 catttgtctt tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat
     7621 aatactcggt taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat
     7681 tatatcgcag cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt
     7741 caagttatct ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc
     7801 cgtacaacaa tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt
     7861 atcgccttga ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc
     7921 gtttgttact gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct
     7981 atgctgaatc aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt
     8041 gtcagcggct catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc
     8101 agcgatattt tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa
     8161 tctcgcatca actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt
     8221 tctgatgcag ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat
     8281 attttgaagg atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc
     8341 actgtagaac gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt
     8401 caaatcgcgg aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc
     8461 aatcaataca ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag
     8521 acgttacggg aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta
     8581 tcaagatctc gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt
     8641 aagtgatgtt cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg
     8701 ttatgatggg atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga
     8761 ccttttgttc atgatcgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt
     8821 gggaagtgtc acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa
     8881 tgttgcaaaa acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat
     8941 tattggtggc gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca
     9001 attagaaaaa atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg
     9061 ccatcaaagc agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg
     9121 tgctttcagt cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac
     9181 atggtttaga aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa
     9241 tggcgcaaaa cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca
     9301 ttcgtgaagg agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg
     9361 ccttgatcgg tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg
     9421 ttactgacaa agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg
     9481 ttaacgaacc aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg
     9541 gacaacgtga attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgatcgata
     9601 cgatcatcaa ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag
     9661 attctacagt tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa
     9721 tcgttgtgaa tgccggtgcg tctcaaccag caccattgct ttatatcgca ccatatgctg
     9781 gtactgcaat gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg
     9841 atctttcaaa acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag
     9901 gtcgtgaagc ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag
     9961 caaaattaag tgatgattta ggtggcggtt caatgactgc cttaccattt gttgaaacac
    10021 aagctggaga tatctctgct tatatcccaa caaacgttat ttcgatcact gatggacaaa
    10081 tcttcttaga aagcgacttg ttctacgcag gtactcgtcc agcggttgat gccggactat
    10141 ctgtatctcg tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt
    10201 tacgcctaga cttagcaagt taccgtgaat tagaagcatt tacacaattc ggttctgact
    10261 tggatgcagc aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac
    10321 aaaaattgca tgcaccgcta gctgttgaaa aacaagtagt gattttgtat gctttgacac
    10381 atggattctt agatagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg
    10441 aatatctgga taccaatcat tcagatatct ttgaaacaat ccgtacaaca aaagatcttc
    10501 cagaagaaga agcgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa
    10561 aaggaagtaa ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg
    10621 agatgaatgg gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg
    10681 agccaaatca cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct
    10741 tcttcacaga agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact
    10801 gctactcagt taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc
    10861 atgctgctca gccgtgctgt gaagaaaact ggttatatcg tcatcactgc agatggcggt
    10921 ttagtcggtg gatataacag ctcgatattg aaacaaacga tgtctatttt agaagaagac
    10981 caccaatcac ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt
    11041 aaagcacgcg gtatcaatct agcatatgag ctgcgcaatc tatctgatca gccaagcttt
    11101 gatgaagtcc gcaaaatcgt aaatatggca acaactatgt tccaaaacga agtattcgat
    11161 gaattatacg tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa
    11221 aaaatgctgc caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat
    11281 atttttgaac cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt
    11341 ttgatctacg gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca
    11401 atgaaaacag caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac
    11461 cgtgcaagac aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca
    11521 ctagaatagt attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat
    11581 cggtcccgtt gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc
    11641 attagttgtt tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga
    11701 attaggggac ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg
    11761 aatggaagtc attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg
    11821 tcgcgtattc aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc
    11881 ggaaagaagc gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga
    11941 aattttggaa acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa
    12001 agtcggacta ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca
    12061 taatatcgcc caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg
    12121 tgaaggtaat gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat
    12181 ggtgttcgga caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt
    12241 gacgattgcg gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa
    12301 cattttccgt ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc
    12361 tgcggttggt tatcaaccaa cattggcaac agaaatgggt caattacaag aacgtatcac
    12421 atctacgaaa aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta
    12481 cactgacccg gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg
    12541 taaattaaca gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc
    12601 tttagcacct gaaatcgtgg gggaagaaca ttataaagta gcaacagaag tccaacacgt
    12661 attgcaacga tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc
    12721 cgatcaagaa aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa
    12781 ctttaacgtg gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac
    12841 agtgaaagga ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt
    12901 ccgaagtgtt ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa
    12961 aggggtgccc catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct
    13021 atgatcacca tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc
    13081 ctaaacatgc accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag
    13141 attcagatac ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata
    13201 atgttgtttc aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg
    13261 cggaacgtgc aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata
    13321 cgaacgaatt gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat
    13381 ctaagcactc ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg
    13441 ttatgacaga agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa
    13501 atttttgtga aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag
    13561 aggttgtaaa atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc
    13621 atattttgcc gttttttgag ctaataccga gggtcagatg attgaacacc gtttattt
//
LOCUS       DACESX010000070        13622 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.070, whole genome shotgun sequence.
ACCESSION   DACESX010000070 DACESX010000000
VERSION     DACESX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.070"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..561
                     /locus_tag="IXF88_001725"
     CDS             <1..561
                     /locus_tag="IXF88_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508458.1"
                     /translation="NYFYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPD
                     IYDDHYDSDGTCKVSIEKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVR
                     GNSIDGVRKVNRSLILEIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRS
                     RRFKLYVLQKDYASNGKYSKKYRIFI"
     gene            599..988
                     /locus_tag="IXF88_001726"
     CDS             599..988
                     /locus_tag="IXF88_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1508459.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            975..3422
                     /locus_tag="IXF88_001727"
     CDS             975..3422
                     /locus_tag="IXF88_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1508460.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            3427..5535
                     /locus_tag="IXF88_001728"
     CDS             3427..5535
                     /locus_tag="IXF88_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1508461.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            5532..6536
                     /locus_tag="IXF88_001729"
     CDS             5532..6536
                     /locus_tag="IXF88_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1508462.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            6555..7466
                     /locus_tag="IXF88_001730"
     CDS             6555..7466
                     /locus_tag="IXF88_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1508463.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            7653..7940
                     /locus_tag="IXF88_001731"
                     /pseudo
     CDS             7653..7940
                     /locus_tag="IXF88_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(8128..8343)
                     /locus_tag="IXF88_001732"
     CDS             complement(8128..8343)
                     /locus_tag="IXF88_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508464.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8602..8769)
                     /locus_tag="IXF88_001733"
     CDS             complement(8602..8769)
                     /locus_tag="IXF88_001733"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1508465.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(9114..10553)
                     /locus_tag="IXF88_001734"
                     /pseudo
     CDS             complement(9114..10553)
                     /locus_tag="IXF88_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(11101..11772)
                     /locus_tag="IXF88_001735"
     CDS             complement(11101..11772)
                     /locus_tag="IXF88_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1508466.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(12121..13572)
                     /locus_tag="IXF88_001736"
     CDS             complement(12121..13572)
                     /locus_tag="IXF88_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1508467.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT         4335 a         2475 c         2590 g         4222 t
ORIGIN      
        1 aactattttt attttgtatt ttgggtagca gcaactttac ttggttatga taaaattcag
       61 attgcaggaa ttcctataca catgcaatat aagttggttt tgagtggaat tttttctgag
      121 gtgttgccag atatatacga tgatcattac gacagtgatg gaacatgtaa agtaagcatt
      181 gaaaaagaaa atttcgatga catcgatgga tatgactctg tgaacttact gattattgat
      241 acttacgata taaaaatgag tgaattatct atggaaaatc aaacatatcc tacgataatt
      301 gttagaggga attcaataga tggagttcgt aaagttaata gatctttaat cttggaaata
      361 aaaaaaacaa tggatgaaat tcaaaaaagt gactttaaaa aagtatttgt tgcttcaaca
      421 tctaatccaa aaaatagtat caatattatt aattcatcgt ttagattttt cggtcgatca
      481 cgtaggttta aactttatgt tcttcaaaag gattatgcaa gcaatggaaa gtatagtaag
      541 aaatatagaa tttttattta agaaggaatt tttccttctt tttattttgg aggaaactat
      601 gaaaaaaata aaaagctaca ccagtatttg ggcagttgaa aaagtcattt atgcgattaa
      661 tgactttcag ctgccttttc ctgtgacctt taaccagatg gcttggtttg ttttatcctt
      721 gatgtttgtc attgtatttg cccatgtgcc tccgctatcc atgatagagg gggctttttt
      781 aaaatacttg ggcattccga tagcggtcac ttggttcatg tcgcaaaaga cctttgacgg
      841 taagaaacca ttgggatttt tacgatcctt tatcagctat cacttacggt ttaaggtcac
      901 ttttgcagga aagaaagtta aagaacagaa gaaacgatgg gacgaaccaa tcaccttggt
      961 aaggagtgtg aattatgtac ccgattaagt atattgaaaa taatcttgtc tttaaccaag
     1021 aaggcgagtg ttttgcttac tatgaattag tgccttacaa ttactccttt ttgtcacctg
     1081 aacagaagta tcaagtacat gataactttc gccaattaat tgcgcaaaat cgtgaaggta
     1141 agatccatgc cttacaaatt gccacagaaa gtagtattcg tgccacacaa gaacgttcga
     1201 aaaaggaaat taccggtaga ctcaaagaag tagcaaagca acgaattgat ttacagacag
     1261 atgcattagt atccatgatt ggcgatagtc agattgatta tcgcttcttt attggcttta
     1321 aactgatttc gacagatgaa gaggtcaatc taaagaatct caggaaatct ttcttttctg
     1381 gacttcaaga atttgtctat ggagtgaatc atcatttgat gggtgatttt gtttccttat
     1441 ccaatgaaga aattcgtcgc tataccaagc ttgaaaaact aatggaaagt aaattggctc
     1501 gaagatttaa agtcaaacga gtaacgccga gtgacttgac gtatttaatt gaacatatct
     1561 atggtgaaaa aggcacaccg tttgaagaat atgaattcca gctaccaaag aaaaagctga
     1621 aatcagaaac gctggtgaaa cgttacgact tattacgtcc cactcgttgt ttgattgaag
     1681 aaaagcctcg gtacttacgg atggaacatg aaaatcatga gtcttatgta gcgtacttaa
     1741 caattaatac cattgtaggg gaaatggagt ttccttcctc ggaacttttc tattaccagc
     1801 aacaacaatt tacgtttccg attgatactt caatgaatgt ggaaattgtg acgaataaaa
     1861 aggccctagc taccgttcgt aataagaaaa aggaattaaa agatttagac aaccatgcct
     1921 atcaatctga taatgaaacg aactccaatg tattagatgc tttggattcc gtggatgaat
     1981 tggaaaccac cttggatcag tcgaaagaat ccatgtataa actaagctat gtggttcgag
     2041 tgagtgctaa atcagtggat gaattaaaac gccgctgtga tgaagtgctc gatttttatg
     2101 acgataccaa tgtaaaacta gtccggccct ttggcgatat gatgggtcta cacaaagaat
     2161 tcttaccctc aagtaagcga tacatgaatg actatattca atatgttacg tctgactttt
     2221 tggcgggact tggttttggg gctactcaaa tgctaggaga attagaaggc atttactttg
     2281 gctataacgt agatacgggg cgcaatgttt acctgaaacc ggcattggca tcacaagggg
     2341 tcaaaggctc agtaaccaat gctttggcat ctgctttcct tggttcgtta ggtgggggga
     2401 aatcttttag caacaatctc ttagtctatt atgccgtcct ttttggtggg caagctgtga
     2461 ttgtcgatcc aaaaggagaa agaggcggtt ggaaagaaac gttgccagaa attgcggatg
     2521 aaattaatat tcttaacttg accagtaagc cagaaaatca aggcttactt gatccctatg
     2581 tgattatgaa gaaaaagaaa gactcagaaa gtttggcgat tgatatcctg acctttttaa
     2641 cagggatttc cagtcgggat ggggagaagt tccctgtgtt acgcaaagcc attcgaacgg
     2701 ttacccaaag tgatcagcga ggattattac aagtaatcgt tgaattgcgt aaagaggact
     2761 caccagtcgc ggaaaatatc gcggatcaca tcgagtcctt taccgattat gattttgccc
     2821 atttgctttt ctcagatggt tcgattaccc agtcgattag tttagataag cagttaaata
     2881 tcattcaagt agcggatttg gttttaccag atgcagaaac cacttttgaa gaatatacca
     2941 ccatggaatt gctgagtgtt tccatgctaa ttgttatttc cacttttgcc ttggacttta
     3001 tccatagcaa ccgagaaatt ttcaagattg tggacctgga tgaggcttgg agttttcttc
     3061 aagtagctca aggaaaaacg ttatcgaata aattagtgcg tgctggtcgg gccatgaatg
     3121 ccggtgttta cttcgtcacg caaaacagtg atgacttgtt ggatgaacgt ttgaagaata
     3181 atattgggct aaagtttgca tttcgcagta ccgatattca tgaaatcaag aaaaccttgg
     3241 aattctttgg attagataaa gaagatgaaa gtaatcaaaa atgcatgcga gatttggaaa
     3301 atgggcaatg cttgatgcag gatttgtatg gtcgagtagg tgtggtccaa gttcatccag
     3361 tctttgaaga acttttccat gcttttgata cccgtccgcc agttcaagag agaatggagt
     3421 gaggagatga aaaagaaact ttttcgtatc tttggaattg ttttaatcag tttggtcact
     3481 ctgataatac ttttgagctt agttggaacg gtagctgagg caacaggtct ggttgatgat
     3541 acagttggag ccggaaatct ttattcccaa tattcacttg gtaactatca gttggatttc
     3601 tttgtggata gttcgtggga ttggctgcct tggaattggg gtgatggcct agggaagagc
     3661 gtaatgtatg gactttatgc cattactaat tttatttgga cagttagtct ctacttatca
     3721 aatgccacgg gctacgtggt tcaagaagcc tataagctgg atttcatttc agatacggct
     3781 gaaagtattg ggaagaatat ccaaacctta gccggcatta ccgaaaatgg actccagacc
     3841 tcgggctttt actttgggtt tctattattg atgattttag ctctaggagt ttatgtggcg
     3901 tatacaggat tattaaaacg agaaactacc aaagcagtcc gggctgtcat taattttgtg
     3961 atgatttttc tactttcggg ttcgttcatt gcgtatgcgc ccacctatat cacaaaaatc
     4021 aatgatttta gctcagatgt cagcgaagcg gctcttagcc ttggaaccga gattgtcgtg
     4081 ccgaattcag aaagccaagg aaaggacagt gtggatttaa tccgagatag cttgttttcc
     4141 attcaagtcc aacaaccgtg gttactcttg caatttgatg attccaatat agaagaagtt
     4201 ggcgaagatc gtgttaacaa aatcctgtct gtaagtccag atgaaaataa aggcaaggat
     4261 cgggaagaag cggtcaaagc ggagattgaa gataatgaca atgcgaatct cagtattacc
     4321 aagaccatga accgcttagg gaccgtggtc tttttggtgt tgttcaatat tggcatatcc
     4381 ttctttgtct ttctcctaac agggattatg ttgttctcgc aaattctctt tattatcttt
     4441 gcgatgtttt tgccgattag tttcttattg tccatgttgc cgacttatga gagtttgggc
     4501 aagaaggcga ttattcgctt gttcaatacg attatgatgc gagcaggagt tactttggtg
     4561 attacgactg catttagcat ctccacgatg tttttcaata tttcggcaac ctatccattt
     4621 ttcatggttg cttttctgca aattgtgacc tttgccggta tctatttcaa attgggcgac
     4681 attatgaata tgttcaatct ccagagtaat gatagccaat caatgggaag acgggtcatg
     4741 cgaaaaccgc aaatgttaat gaatcgaaca ttgcggcaac tcaatcgaaa tgtgggacgg
     4801 actttggctt ttggtggtgg tgcagcagtt ggaaataagt tggcaaagga acaatcaaaa
     4861 tccaaattta agccatccgg ttcttctcta cgaaagaatt cacgattacc gaacgatcac
     4921 gagataccat ctgacttgaa taaggaaaag ccaattccaa ataataagaa acaatcccga
     4981 atgaatttgg ccgggagaaa agtcggcaag gttctggata cccaagcttt ggtgaaagat
     5041 aagaccaaac aagtaaagga tggagtacgg aatacgccaa ccaacttgaa atacaacttc
     5101 cataaaggaa ttgaaaaaac gaaaaaagca ccggaagagt ttaagcgagg actggtccaa
     5161 gaaaaagcaa atcgaggaga gttgcgagaa aaacaacaac aacgtagaga cgaaaaaata
     5221 gctgaaaagc gcaaagtatt gaacgaaata gggaatcctc atgggaaaag aagggaaaat
     5281 gttccgataa aagcaaaggt ccaatctcaa aaggatagag caccaaagcg gattgtgaga
     5341 gcaaattcga atcccgaaat caaacgaaaa ttcgctagcc aagaaatcat ttcaaaagga
     5401 ataaaccaat cctcaagtaa gaaaaattct tttcaacaag tgaaacggcg gtctactctg
     5461 tccaaacgaa ccaatcgaaa agtacaaaaa agtttgaacc ctcatcccaa accaaggtca
     5521 ggtgagaaaa aatgaacgta aagaaattaa gtgccatttc gttaactttc ctaacaatct
     5581 cttttatggg tatcttgatg tgtgtgggct tattgtttgg tgaagacagt gaaggaagta
     5641 gtagttcagg tgctcctcct ggggggagta gtgtctcaga agaggttttg aaacaccgtg
     5701 caatggtgga aaaatatgcc aaagaatcgg atataacaga gtatgttccg attcttttag
     5761 cgattattca agtggaatct ggtggcacaa tggaagatgt gatgcaaagt tctgagtcag
     5821 ccggtttacc accgaattca ttaagtaccg aggcatccat caaacaagga tgtctctatt
     5881 ttgcgtcttt ggttaagcgt tccaaagaat taggttgtga tcaagatagt atcattcagg
     5941 cctataactt tggcggtggt tatttggact atgtggcaaa aaacgggaag aagcatagtt
     6001 ttgctttagc tgaatctttt tcaaaggaaa aatcttgcgg tcaaaaaatc gattacccta
     6061 atcccatcgc gaataaggaa aatggtggat ggcgctataa ctatggcaat atgttttatt
     6121 gcttattggt gaagcaatac ctaactacaa cacagtttga cgataaaaca gtccaaggga
     6181 tttttgacga ggcgtttaag tatgaaggaa cggcttatgt atttggtggg tcaagacccg
     6241 aaacaggatt tgattgtagt ggcttgactc aatggtgtta tgccaaagtt ggtctgaaac
     6301 ttcctcgggt ggctcaagat cagtatgatg ccatgaccca tattgaccta aaagatgcca
     6361 aaccagggga tttggttttc tttcattcga cttatgatgc cggtacttat gtgacccatg
     6421 tcggtattta tgcaggggag aatcgcatgt ttcatgcagg agatccagta ggctggacga
     6481 atctaacaga aagctattgg cagcagcatc ttatcggtgc tggacggtac aaataagaga
     6541 aaagaggaaa aagaatgaag ataaaaattg aacggaatca aaaggaaaag agcctaaaaa
     6601 agaaaaaaga acgagtcgta tccgtgggta agcatcgaaa aatggtgctt gccctttggc
     6661 ttttattatc ttgtagtctt acttttggcg tttataaaaa tttcaccgcc attgatcaac
     6721 atacaactca tgaaaaagtg gttattaaag agaaagtagt gaatacttct ggtattgaaa
     6781 actttacgaa ggatttttcg aaagaatatt tttcgtggaa aaataacaaa gaagcaattg
     6841 aaaagagaat gaacaacctc gaacagtacc ttacagaaga aggtttcgct ttaagtcaag
     6901 atatggttcg agcagatatt cccactaatt ccgaagttca gtcagtaaaa gtcttagatg
     6961 ttgaaaagag ctcggaggaa tttgtcgttt catttttagt ggagcaaaag attacagagg
     7021 gaaaaaaggc acaatcgatt tcttctgcat accgagtgac gatctttgaa gatgaaaaca
     7081 ggaattatat agtaacgagt ttgccaacta tgattgcaaa acctgatagg gcaaaataca
     7141 agacaaaaca agtagaaaat gattcgaaaa ttgatgccaa aactacagaa gaaatcgctg
     7201 agtttttgga aacgttcttt aaattatatc catcagcttc tgaaaaagaa ttggagtatt
     7261 atgttgaaaa tgatgcgatg cgaccaatca atattgactt aaagttttcg aagttagagg
     7321 atccagttta cctacaaaac ggaaaaaaaa ttctagtgag tacaaagata aaataccttg
     7381 ataatgtatc aaaaaccact agctactttc agtacaaatt aatattacaa aaaaatggaa
     7441 actggaaaat cattgatgtt aaataataat gactcaatct aaatttatga gaagttataa
     7501 gtagtttaac ttatttcagg cagaagtcgc ccatcctcta taggtgggtg atgaatgccg
     7561 ttcggtaact cgaaaaccga caattaagac atagtgcctt tttattttaa tccatgtcat
     7621 aatcagtcta tataagaaat ggttgatata ccatggaatt agatacaaat aatcattcat
     7681 tatttcttct ttgttaccac cttattttag tagtgaaaca ttgtaaacat ctcattgatg
     7741 atgtaatgtt agcttatgca aaaaactatc tttgaaagaa tatcagaatc acatcacatt
     7801 accttagttg agtgaaatcg tgataaagac tactttcata ttatgctcaa agcacaacct
     7861 aaaaccgaat taacaaaatt cagcaatgcc taagctcatg tctgataaaa tgagactttc
     7921 taagggtcaa aaatttcctt tgaatacgat cattagctta gataaaagta aaaaccttag
     7981 aactacgggg atagcttggt taataagaga tatcgctgtt aataaagaca taagcttgca
     8041 agtatgctct attcccaaga atctcccact tcaagcacta aggatcgtta aattcagcta
     8101 agtggcaagt agttcactag acgataatta tctccatatt tcaatctcag cttttccatt
     8161 tttcatagca aattccgtta tttttatagc atctaaaaga ttcagctttt ccactgatct
     8221 ttcaccactt ttcagctttt ttattgtgct taaattgata ccagtacctt ttgatattga
     8281 attggaagta aaatcattaa gggttttttt tattaaattc atatccgcaa ttccagcgtt
     8341 cataagaatg aagattctcc tctcatagac tactttgaaa atggtactac agtggctgtt
     8401 tatatatttc agtaggttac tgtagtggtc attttattat aattagaaaa ggaacctaaa
     8461 tcaaaaatgc taaagaaaat tttcatagta ttaaacctat catgactaaa gccacggaat
     8521 tcttgataaa agagtatact tgcaagccta tgtctttact aacagcgata tctcttatta
     8581 accaatctat ccccgttgtt cctaaggttc ttgatgtttt ctaaggcaat gcttgtatac
     8641 atagcccttg atcaaaatat tgatactaac gttgacgttt cgatcatgat gtgtacggca
     8701 aataggacaa gtccattctc gaattgtgaa tgacttctta ccatctgtgt gtccacattc
     8761 tgaaccaatt tgactagacg gaaatcattt atctatcttg atgatttttt tgacagaagt
     8821 atcgaaaatc tttacataat ctgggtgttg cattcttatg cagatgagag cgttagctcc
     8881 gtattcataa ccagttgcct ctaaaacgta gtccatcggt ggatacctag cttgcggttg
     8941 ccagtggctt tcgcagtctt cttgtaatca aatcgagcat ctaattgagt actagtattt
     9001 tgaagataat acttgagttt ttcggttttc agaagttcac ttcatcttcc atcatccatg
     9061 gaaatcccac tcacccatac gcaggcctcc aaaaattctt caacaatggt ttttcagaca
     9121 tttttctgaa tacctccttg ttttttgata cgccagatag tcaggaaaaa ggcaacaaaa
     9181 cacaaaacaa agctgcctaa aaaggtaatc ttcattccat ataaaaagac atccggccga
     9241 tctgcgatat agttagttac tcgttggccc atcttagcag acatcgcttg atataagacc
     9301 gtcgtagaaa gcgaaatccc gatgaccatc cccaaatttc gggcaaagga attcatggag
     9361 cctgccagtc ccaaattttc gatagtgaca gaactcataa ccatcgtatt atttggggac
     9421 tggaacaagg cattgccggc acccatgatg gcagtagcga caatgtacag ccacaaagga
     9481 gagtccactg acaaaaacat atacatgact tgggttaccg acaataaacc caagcctact
     9541 aatacaaaaa tctccgggcc aacccggtcg gtcaggtatc cgcttaaagg cgccccgacg
     9601 accatcatca agggaaagac catcatcaac agtccggcat agctaggact caacccgcga
     9661 gcattttgca gataaaaagg aatcactaca ttaacaaaga agttggaaga aaaaatcaac
     9721 aaggctgtaa tcaagctcat ggtaaaaatc ttgttcttaa aaataccgaa agaaatcaat
     9781 ggagaggctg ctttcttttc aacttttaaa aagctgacaa aggcaatcac tgaaattacg
     9841 aacagtacaa gggggactgg atggaaaaag ccactttctt gccccaaaaa gattgttcca
     9901 aaaaggctga taatcgtagc cgcaatggca gcaaagcccc ctccatcgat tgtatggccg
     9961 ctcatggtga tatcttttgg taagaatttt tcagcaatca acatcgttac gattcccacc
    10021 ggtacattga tccaaaaaat gtacgcccaa gaaaattggg acaaaatcaa gccgccaaga
    10081 cctggtccag caatcgagcc taatgaaaca agcgcaccga ttgctcccaa tgcccgacca
    10141 cgttcatgta tgggaaagac ttccgtgatg attccggcat ttgtagccat agtcatacta
    10201 gcaccaatgg cttgtaccac ccgtgcgaac aattaaatac ctagcgaatg attgaaccca
    10261 cacaacagcg agcccaagac aaagatcagc gtaccaatcc gaaatacttt gattttccca
    10321 cagctatcgc caattttacc aaacagcaac aaacaagaac agacaatcat cagataaacg
    10381 gacactaccc attccgcttg attcattggt atgcccatat catctgagat ggtaggtagt
    10441 gcaatattga caatactgcc atccaatgtg gacatgaagg taaacataga tatagcaatc
    10501 aaaatccacc agcgattttt ttggactttt gcatcttctt gataactatg cataatagga
    10561 tttcctttct aatgatcgtg tagctaagaa caatataacc tatgtctact gcccaacaac
    10621 ttctcgcgac tctagccata ggaagcttga gtaatttcga tttgttattc tcatgtaacc
    10681 atgtaagttt gagatgcttt atttgaactt actctatagt aactagtgat aattcgcttt
    10741 actttcttct agcaatttct aaaagtcgac ttcattgttc ttcagattaa tagtgtgagc
    10801 tatctcgtga gacgtaagaa aatttccatt ctactttttc ccaatttttt catatttatg
    10861 gtgttgcacc tatttgtttt ccaatttgct tttcaaatac attgctatga tattcaattc
    10921 cataggatta tactaaatgt caatagtatg aataatatat tatagatgtt tatatatcac
    10981 tgttaattat tatccacaat aattaatgat tacctcgata tttgtcaatc aaaggttgaa
    11041 acaatttatt ttcagcatct tttctaactt gagctgcact cttaatagaa tcatacgatc
    11101 ctaaatagta cctctttttt ttaaagttat atacgcaatc catttctctt tagaacgata
    11161 aactccctta acaccacttg tattattttt cggtaatttt gacgttagac actctattac
    11221 tgtcccttca acacatgtcc ttgtttcttg cctttttaaa catccacaac tttgagtatg
    11281 tccaaaaaat aagttactat aacttatctc acacatactg ccacactcac attgacaaac
    11341 ccattttgtt ttccatgaac agttattgtc caaaggatac tttgcagtaa gtaatccgaa
    11401 ttttttattt tcaatgtgat gcttttttga tttcaaacat ccacagcttt ttacatcacc
    11461 ttttagaagt cgctttgccg atactaactt ttttctccca cacagacatt gacattccca
    11521 aacaacagtt ccttcacttc gcagtttagt tggcttgatt gcaactaact taccaaaagt
    11581 tttatttgtc aaatttttat atttatgcct atttttataa tttccacatc cacaactttt
    11641 ggttctacct gatagtaagt ttgatcgata tacaatcgtt tcattcccac agtcacatag
    11701 gtaacgatat tttttatatc cattatgagt aatctcctca acaacagtaa ggtgaccata
    11761 tctttccttc actgcaccta ccctcccaag aaagaagtac catataattt caattaactt
    11821 ttaataaatc agaaaaaaat attttcaatt tttcttagtc tgattttatg actaatttat
    11881 aatacaagac tgaacgatac gtacacgaaa tcctcaagat agataaattg ttttcatcta
    11941 gtaaaacctg ctcaaaatat gagcataaag acggcaataa tacactcgat attcgataat
    12001 ggacttgttt tatttgtcaa actcatcgta atcgagatat caattccagt ctcaatatcc
    12061 tgatcaaagg tctaagaatc caaccatttt cttgttactt tgtcagattg attacctttt
    12121 ttagtctccg aataataagg ttttcggatc aactaacatg ctctgagaaa gactactatc
    12181 tttgcaaatt tccaaatgaa ggtgtgggcc agtactattc ccagtacttc ctacatagcc
    12241 aattatttgt ccttgcaaaa cattatctcc agtttttact aggtattgtt gctggtgagc
    12301 atataacgcg gttgatccat cttcgtgttg tatcaccacg taatttcccc aagagtagtg
    12361 aaactctgct ttaacaacct taccagcttt aactgcatga atcggctcac cctgtgtagc
    12421 tgccatatca agtcctcggt gaaattcatt tccacgattt ccaaacaaac tggttattga
    12481 ataattatga ataggcactt ggtatccagt cttactcatt tgtggacctt taacttcttt
    12541 atcatagttg gttaaatcat aggttttaat taattcattt agcttcaaat agtattgtgt
    12601 atctgttgca taccgtcctg taaggaattt agttgcttct cgatatgttt tactattagc
    12661 ttttaatgct cctgcataaa aatctttatt tccactaatt cccttagtta ataatttggc
    12721 ataatcttca aaactttcct catagttttc atacactcta aattttgatt gggtcgtata
    12781 aagcgttcca ttacccaaat cttcgtttgt taccataatg acaaagtttc cattataagt
    12841 tccttttatc ccaaataaat tgtaattcgg tgcctgagct aactggcttt gaccactggc
    12901 agattctaag atcgcttgtg caatcataac agaagcatat aaatcatatt tctgaccaat
    12961 ttttctcgca gactcaccaa ttttcataat aaaattttct acagattcat ttttgtcaaa
    13021 atgaatttct tcattaccag atagtattgg ttgtgattgg aaagattctg ttgattctat
    13081 ttgctgtggt gtcgaaaaat tttttgtttg tccttccgtt tttggcgctt ccttttttga
    13141 agactctata tggtttggtc ttgtcacttt cgaagaagtt atttcacttg tactacttga
    13201 agttgttgaa gattcggcac ttgaaatcgt actactttca cttgacgaag ttgttgaaga
    13261 ctcggcattt gaagtcgtac tactttcact tgacgaagtt gttgaagact cggcatttga
    13321 agtcgtacta ctttcacttg atgaagttgt tgaagactca gcatttgaag tcgtactact
    13381 ttcacttgat aaagttgtca aaagttcact actctctgct gttacatcgg aattactcat
    13441 ttcagtcgtc tcggttcttt cttttgcaat tttgtatgtt gaattttcgc ttgccaacac
    13501 atggaaaggt gttgctataa agagttggga cattaatata actgctgatg caagttttaa
    13561 agattttttc atttgattta cctctctttt ttctctttaa tagtgttttc tattaaaaat
    13621 ta
//
LOCUS       DACESX010000071        13148 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.071, whole genome shotgun sequence.
ACCESSION   DACESX010000071 DACESX010000000
VERSION     DACESX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.071"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(78..150)
                     /locus_tag="IXF88_001737"
     tRNA            complement(78..150)
                     /locus_tag="IXF88_001737"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="IXF88_001738"
     CDS             531..1100
                     /locus_tag="IXF88_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1508468.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="IXF88_001739"
     CDS             1087..1947
                     /locus_tag="IXF88_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1508469.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /locus_tag="IXF88_001740"
     CDS             2137..2841
                     /locus_tag="IXF88_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1508470.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="IXF88_001741"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="IXF88_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1508471.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /locus_tag="IXF88_001742"
     CDS             4678..5994
                     /locus_tag="IXF88_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508472.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="IXF88_001743"
     CDS             5995..6864
                     /locus_tag="IXF88_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508473.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="IXF88_001744"
     CDS             6919..7728
                     /locus_tag="IXF88_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1508474.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="IXF88_001745"
     CDS             complement(7831..8460)
                     /locus_tag="IXF88_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ1508475.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9922
                     /locus_tag="IXF88_001746"
     CDS             8630..9922
                     /locus_tag="IXF88_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1508476.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9998..10660)
                     /locus_tag="IXF88_001747"
     CDS             complement(9998..10660)
                     /locus_tag="IXF88_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1508477.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10885..11169
                     /locus_tag="IXF88_001748"
     CDS             10885..11169
                     /locus_tag="IXF88_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1508478.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11220..12845
                     /gene="groL"
                     /locus_tag="IXF88_001749"
     CDS             11220..12845
                     /gene="groL"
                     /locus_tag="IXF88_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1508479.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4371 a         2265 c         2833 g         3679 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9061 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9121 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9181 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9241 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9301 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9361 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9421 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9481 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9541 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9601 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9661 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9721 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9781 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9841 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
     9901 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
     9961 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10021 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10081 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10141 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10201 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10261 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10321 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10381 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10441 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10501 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10561 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10621 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10681 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10741 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10801 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10861 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    10921 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    10981 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11041 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11101 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11161 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11221 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11281 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11341 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11401 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11461 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11521 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11581 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11641 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11701 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11761 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11821 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    11881 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    11941 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12001 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12061 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12121 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12181 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12241 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12301 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12361 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12421 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12481 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12541 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12601 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12661 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12721 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12781 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    12841 tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta gtgaaagaca gataaaaaga
    12901 ggctggaaca gaagcgttta actccaagaa ataagaagaa attcacggaa attgcttttc
    12961 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ctgtttatac
    13021 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttttgtgtgt
    13081 aattataatg aaaatagtta tttttaatag ttataatatt ttatctatta aaagtaaata
    13141 tccattca
//
LOCUS       DACESX010000072        13085 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.072, whole genome shotgun sequence.
ACCESSION   DACESX010000072 DACESX010000000
VERSION     DACESX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.072"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(79..2199)
                     /locus_tag="IXF88_001750"
     CDS             complement(79..2199)
                     /locus_tag="IXF88_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1508480.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="IXF88_001751"
     CDS             2429..3607
                     /locus_tag="IXF88_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1508481.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="IXF88_001752"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="IXF88_001752"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1508482.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="IXF88_001753"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="IXF88_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1508483.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="IXF88_001754"
     CDS             complement(4960..6213)
                     /locus_tag="IXF88_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1508484.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="IXF88_001755"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="IXF88_001755"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1508485.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="IXF88_001756"
     CDS             7790..9136
                     /locus_tag="IXF88_001756"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1508486.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="IXF88_001757"
     CDS             complement(9444..9830)
                     /locus_tag="IXF88_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1508487.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            10709..10921
                     /locus_tag="IXF88_001758"
     CDS             10709..10921
                     /locus_tag="IXF88_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508488.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="IXF88_001759"
     CDS             complement(11199..12998)
                     /locus_tag="IXF88_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1508489.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaa
//
LOCUS       DACESX010000073        13051 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.073, whole genome shotgun sequence.
ACCESSION   DACESX010000073 DACESX010000000
VERSION     DACESX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.073"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            145..1605
                     /locus_tag="IXF88_001760"
     CDS             145..1605
                     /locus_tag="IXF88_001760"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1508490.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            1927..2463
                     /gene="cysE"
                     /locus_tag="IXF88_001761"
     CDS             1927..2463
                     /gene="cysE"
                     /locus_tag="IXF88_001761"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1508491.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            2460..3872
                     /locus_tag="IXF88_001762"
     CDS             2460..3872
                     /locus_tag="IXF88_001762"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1508492.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            3869..4264
                     /locus_tag="IXF88_001763"
     CDS             3869..4264
                     /locus_tag="IXF88_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1508493.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            4257..5117
                     /gene="rlmB"
                     /locus_tag="IXF88_001764"
     CDS             4257..5117
                     /gene="rlmB"
                     /locus_tag="IXF88_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1508494.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            5121..5651
                     /locus_tag="IXF88_001765"
     CDS             5121..5651
                     /locus_tag="IXF88_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1508495.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            5728..6306
                     /locus_tag="IXF88_001766"
     CDS             5728..6306
                     /locus_tag="IXF88_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1508496.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            6421..6666
                     /locus_tag="IXF88_001767"
     CDS             6421..6666
                     /locus_tag="IXF88_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508497.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            6759..7205
                     /locus_tag="IXF88_001768"
     CDS             6759..7205
                     /locus_tag="IXF88_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1508498.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(7264..7725)
                     /gene="mscL"
                     /locus_tag="IXF88_001769"
     CDS             complement(7264..7725)
                     /gene="mscL"
                     /locus_tag="IXF88_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1508499.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            7977..9245
                     /locus_tag="IXF88_001770"
     CDS             7977..9245
                     /locus_tag="IXF88_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1508500.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            9238..10524
                     /locus_tag="IXF88_001771"
     CDS             9238..10524
                     /locus_tag="IXF88_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1508501.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            11302..12186
                     /locus_tag="IXF88_001772"
     CDS             11302..12186
                     /locus_tag="IXF88_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1508502.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            12395..12973
                     /gene="pgsA"
                     /locus_tag="IXF88_001773"
     CDS             12395..12973
                     /gene="pgsA"
                     /locus_tag="IXF88_001773"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1508503.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
BASE COUNT         4397 a         2337 c         2828 g         3489 t
ORIGIN      
        1 gaaattcaac cagttggctt tactatttac cttagaaaag gtaaaatttg tgctgagtgg
       61 ctttaaataa atgatatttg agaagccgta actgacggaa tttttgctgt tagtcaaagt
      121 ctagtttgaa agaagaggaa gaaaatgacg aaagtacgcg tacgctatgc accaagtcca
      181 actgggcacc tgcatattgg aaatgcaaga actgcattat tcaattattt gtttgcccgc
      241 cacaatgatg gagactttat tattcgtatt gaggatactg accaaaaacg taatatcgaa
      301 gatggcgaaa aaagccagtt ggaaaacctt gcttggttgg gaatgaattg ggatgaatct
      361 cctgaaaatc ctggagaata cggcccttac cgtcaatctg aacgtaaaga aatctatcaa
      421 ccgctgattg atcagctgct tgctagcaat cgagcataca aatgctattg cacagaagaa
      481 gaattagaag cagaacggga agcacaacgc gcaagaggag aaatgcctca ttacgcagga
      541 acatgtgcga acttgacacc tgaagaacaa gcagaaaaag aagcacaagg tctagaatct
      601 gttgtacgtt tccgtgtgcc tcgcaataca gaatacgcat ttacagatat ggtgaaaggc
      661 gctatttcgt tcgaatccga taatatcggt ggcgactttg tcatacaaaa acgcgatggt
      721 atgccaactt ataattttgc tgttgcggtc gatgatcatt tgatgaaaat cactcacgtt
      781 ttacgtggag atgaccatat tgccaataca ccaaaacaac tgatgatcta tgaagcattt
      841 ggctggacac caccaacctt cggacatatg acattgatca tcaacagtga aacaggtaaa
      901 aaactaagca aacgtgacga atcaatcttg caatttatcg aacaatatcg cgaattaggc
      961 tatctaccag atgcaatgtt caactttatt gcattattgg gctggtcacc tgtgggcgaa
     1021 gacgaaatct tcgaacacca agaattgatc aaaatgttcg atccaaaacg cctaagtaaa
     1081 tcacccgcag ctttcgatgc gaaaaaactg caatgggtaa acaaccatta catcaaagca
     1141 atggatctag atgcatttac tgacatgtgt ctgccttatc taatcgctga tggacgtgta
     1201 gcagaaaacc cagatgaaaa gacaatcgaa tgggtgaaga agatcgttag cctgtatcaa
     1261 cctcaaatga gctatgctgc agaaatcgtt gatctgtcta cgctattttt caatgaacat
     1321 cctgtattga atgaagcagc aaaagaagta cttgctggtg aaacagttcc tactgttctc
     1381 cacgcattca aagcacagct agaacaaatg gaagtagtag atgctccaac tgtcaaagct
     1441 gccatcaaag ctgttcaaaa agaaactggc gtaaaaggaa aaaatctttt catgccaatc
     1501 cgtgtagctg tatcaggaca aatgcatggt ccagaattgc ctgaaacgat cgaattgctt
     1561 ggtcgtgaaa aatcattagc acatttaaat caagtactga actaaacacg atgaacagaa
     1621 aaaagtaagc agctgatcat tacaagagag ataccgggtg gtgcaaggta tcaatgaatg
     1681 gttgccgaaa tgcctctggg agtggtcttg gcgaaacaca gaaaacagga aaaactgttt
     1741 tataggaaac aaagtagcca agaacgtata acaggcgtta gctgtcagag ataatcattg
     1801 attatgaaga aaagtggaac cacgcggaaa gcgtcttttg ataaggaaac ttatttaaaa
     1861 ggcgcttctt ttctttataa gaagatgatt tcctacataa aattcttacg aaaggagtgg
     1921 aaagtcatga gttggtttac ccgagcgatt gatgcagcaa agagaaacga tccggctgca
     1981 cgttcgacag ctgagatcgt tttgacatac cctggttttc atgccttgtt ttggcatcgt
     2041 ttctctcatt ttttatatca gcaccggctc tttttattgg cgaagatcaa tgctcaattc
     2101 tggcgtttcc taactggtgt tgagatccat ccaggagcaa cgattggaac tggcgtgttc
     2161 atcgaccatg gaatgggaat cgtcattggc gaaaccgcag aaatcgagga cgatgtgatt
     2221 ttattccatg gcgtaacatt aggcggcaca ggaaaagaaa cgggtaaaag acacccaact
     2281 gttaaacaag gagccatgct ctctgcgaat gcgcagatat taggaccggt aacgatcgga
     2341 aaaaacgcca aaatcggtgc aggtgccgtt gtgttgaaag atgttccaga cgacgctaca
     2401 gcggtaggag taccagcaaa agtcgttcga atcaaaggag aaaaagtgag gagatcagaa
     2461 tgataaaaat ctacaataca ttgaccagaa aaaaagaaga attcatccct atacaaaagg
     2521 gacatgtcgg tatgtatgtc tgcggaccga ctgtgtataa ttatatccat atcggaaatg
     2581 ctcgtagcac cattgctttc gatacaattc gacgctattt tgaatatcga ggctataaag
     2641 tcgatttcgt atcgaatttc acggatgtag acgataaaat catccgtaca gcaaacgaat
     2701 taggtattac agcaccagaa gttgctgatc gtttcatccg agcatttgaa gaagatacga
     2761 aagcgttgaa tgtcgagcct gctacactgc atccaagagt catggatcat atgccagata
     2821 ttttagcatt tattcaagtg ttggttgaaa aaggctatgc ttatgaatct tcaggtgatg
     2881 tgtattaccg tacccgtaaa tttgaaaatt atgggaaatt aagtgaccaa tccatcgatg
     2941 aactggaagt cggagcaagt cagcgtacag gggaagaaca gcagcaaaaa gaagacccat
     3001 tagatttcgc tttatggaaa caagcaaaac ctggcgagat ttcttgggat tcaccatggg
     3061 gcaaaggacg tcccggttgg catattgaat gttccgttat ggcaacaaaa catttaggag
     3121 aaacgatcga tatccatggt ggcgggcaag atttagaatt tcctcatcat gaaaatgaaa
     3181 tcgcacaaag tgaagcgaaa acagggaaaa aattcgctaa ttactggatg cacaatggct
     3241 atgtaacgat tggcgaagac gatgaaaaaa tgagtaaatc tctagggaat ttcatcactg
     3301 tacatgaaat gatcaaaaac gttgatccac aagtcttgcg tttcttcatg gcgaccacac
     3361 aataccgtcg tccgatccgc tacagtgaaa cgacgatgaa agaagcagcc accaatttac
     3421 aacgactgaa gaatacgttt gacaatttga aattccgcca agaaactgcc tcagagaatt
     3481 tggcagatga tgcggataaa atcgctttgt tagaaaattt agaacagcga ttcatcgaag
     3541 aaatggacga tgattttaat gcagcaaatg ggatcacagt ggtctatgag cttgctaagt
     3601 ggctgaacac ttatctagac cagccgaaag tttctgccgc tgtcttggca caaggagaag
     3661 ctttattctc gcaatggctt tcgatcttcg gtattttatt tacagcagaa gagttattag
     3721 atgaagagat tgaacaattg atcgaagaac gaaatcaagc aagaagcaat cgtgattttg
     3781 tccgaagcga cgaaattcgt gatttgttga aagaaaaagg gatcttatta gaagatacac
     3841 cgcaaggaac ccgatggaga agagaagcat gagagattac acacaattga acggtttagc
     3901 cttggcttat gttggagatg cgatttacga aatctatgtt cgtgaccacc tgattgatca
     3961 aggagaaaca aaacccaata agctgcatcg tgcggcaacg aattatgttt ctgcgaatgc
     4021 acaagccttt ttgattcagc aaatgctgga agaacatctt ttgacagaag aagaagaact
     4081 ttattatcgc agaggaagaa atgctaaaag ccatacgagc gcaaaaaatg ctgatgttac
     4141 gacgtatcgg gtcgcaacag ggttcgaagc gctcatgggc tatctgcatt tgacaaaaca
     4201 aaccgagcgg atggaagaat tgatccgctg gtgtattcag aaagttggtg aaaaaaatgg
     4261 gtaaagaaag aacaaacaaa catcaatcga aacgtggatt tgaagacaaa aaacaaaata
     4321 gatattcaaa taaaaagcaa actggacgaa atcagtcagt ttctgatcaa aatgaagaag
     4381 cattagaaga taatttcgtc tttgggcacc atgccgcagt agaagcgcta caacaagggc
     4441 gagggaataa actctttctt caagaagatt cacgaggaga aaaagtagaa gaactgaaag
     4501 aaatcgcacg tgaacaagct gtgccagtca aatgggttcc taaacaaaaa ttagaaactt
     4561 tgagcgatca cggcgtgcat caaggagtcg ttcttgcgat tacaccttat gaatatttat
     4621 cccttgacca actgattgaa cgaacaaaag agaaaaaaga agaccccttc tttttgattt
     4681 tggatagcat cgaagatcca cacaattttg ggtcgatttt acggacagca gacgcaagtg
     4741 gcgtcgatgg tgtgatcata ccaaaacacc gagcagttgg aatcacacct atcgtaacaa
     4801 agacttccac tggggcagtc gaacatgtcc ctgttgcacg cgtgacgaat ttggtacaag
     4861 ctgtgcaaca gctcaaagaa acaggatttt gggtatttgg cacagatatg accggcacaa
     4921 acttccaaaa atggaatgcc aaaggatcaa ttgctttagt cattggtaat gaaggcaaag
     4981 gaatgagtcc aggattgaaa aaagaaatgg atgaattgct gacgatcccg atgaccgggc
     5041 acgtccagag cttaaatgcc agtgttgctg ctggtttgct tatgtatcag gctttttccc
     5101 aacgtcaggg ggagtagacc atgaaaaaac agctgttgat tgtagacggc tacaacatga
     5161 ttggttcgtg gccggaactt gtccagctta aaaagcagga caaaatggcg gatgcaagag
     5221 aagctctttt acatcgttta tcaaattatg cgaaatatga aggaatggaa gtcatcgttg
     5281 tcttcgatgc ccaactagtt ccgggcatcc aacaaaatta taagaaatac cagttggatg
     5341 ttgtttttac gaaagaagac gaaacggctg acagttatat cgagagaatc gctggtcagc
     5401 tgaatgaccg tttgacacaa gtgacagtgg cgacaagcga tttagcagaa cagtggatga
     5461 tcttttccca aggagcatta cgaacttctg cttatgaatt atataagacg ataaaaaaaa
     5521 cggaaaaaac aatagcccaa gaaaataaag atatgcattt tattgatttc cgacgtaatt
     5581 ctccatggaa tgaagagcag ttaaaaaaat tgtcccaaaa attggacgat ttaatgagaa
     5641 aaaataaata acatatcgtt ctaatcagtt cgatgaatca ttgatgataa gattcctcca
     5701 taaacgaatt tatggaggaa ttattttatg tctgaggagt tattgcgcgc tgcacgtaat
     5761 ggtgacgaag aagcatttgc tgccctgtat caaaaatatt ttccactttt actacgtttg
     5821 aaaaacacct attatttaag aaattatgtt tctgaggatt gggaacaaga agcaaggatc
     5881 gctttatatc aggcattgag aacttacgaa aaaagcaaag gtgttagctt tggaagttat
     5941 tataaaagca ttataagcaa tcaaatctat tctctgttga ggaagcagaa cgcgctgaag
     6001 cgtcgtgatc aaaaagatga agtctcaatc gaccaaaaaa tagagacaga aggaccagac
     6061 tttttagcag agatcacatt gcaagaacct ttagcgattc agcatctttt attaaaagaa
     6121 gcaatcgaaa attgcgaaat ccagttttca aaaatagaag ctaaaatttt tcattattat
     6181 atgcaaggcg ttgattttgt agacatggct gaatgtttag caatggatca caagcaagta
     6241 atgagcgctt acgggagagt aaaactcaaa ataaaaaaac acttgaaatt ttataatgag
     6301 gaataattta gaaaaatctt aatgagcttc ttatttattt ttattgcatt tcttttttta
     6361 taatggtaaa cttgaaaatt ttaaaaatat gtggtatagt tgatagtaga ggtgaactgt
     6421 atgccaacaa ctttagcttc aatcaagaaa gatttagaag gtcgtatcgg cagcccaatc
     6481 atgttggttg ctcagacagg aagaaagcgt caaacagaac gccgcggtgt tttaacagaa
     6541 acctatcctt cagtattcgt tgttgatttg gatcctgaag aaaattcttt tgaacgagta
     6601 tcttacagct attcagatgt acttactcgg acagttgaga tcgaattttt aactgaagca
     6661 gtttaaaaag caaacgagcc tgagattcgt aaggttcgtt tgcttttttg tgtgcgaatc
     6721 ggtatgctaa gaataaaaaa gcagggggcg tttgcttagt ggaatatcag gtaaagaaat
     6781 ttgaagaatt gtcaacagca gagttttacg cgatcgtcaa agaacgaatc gcagtttttg
     6841 ttgtggaaca acagtgtcct tatcaagaag ttgatgacat tgatggacaa gcttggcata
     6901 cgttttttca agatactgat gggaagatcc ttgcctacac gcgagtatat gaagaagcgg
     6961 agagtatcca ttttggacgt gtccttgttc atcaggacaa ccgtaaagat ggtttaggaa
     7021 gaaagattgt tcaagaaacg attaacatga tccagaaaaa ttttccagaa aaaccaatcg
     7081 tgatcggcgc acaagaatac ctacaagctt tttatgagtc gtttggattc aaggcaatct
     7141 cagatgtgta tttggaagat gatattccac atatcgacat gaaaaaggaa caagtagcaa
     7201 gttaagaatc taaaaaaaga aagccgacac tgaaaatcgg ctttcttttt tttagattct
     7261 ttattattta gaagtatcgg aaagatttgt ttgctctact ggatgtgtat tgtcttctgc
     7321 cacttgtttg gcaagaagat cgcgtatgtc actaagataa tcttctgcag taggagtttc
     7381 ttccacttcc acttgctctt ctttggagcg taaattcttc gcacggtttg ccgctttgac
     7441 aactaagaat aagacaaaac cagtgatcag aaatgtaatg atagcactaa taacgttacc
     7501 aaacgcaaat gtgacgcctt ttgtcggcga ccagtcaaga attgaaagtg ctccttctaa
     7561 atcagaagta cctgtgatca ttgcgacaaa ccaacctatt agtggtgtga tcaagccttg
     7621 caccacttgg tttacgatgc ttgtaaacgc actaccaata acaacgccga cagcaagatc
     7681 caagacatct ccacgcatga tgaactcttt gaattctttg atcatgaatg accctccctt
     7741 cttttatttg tctttagtat atcgaaaaat aaaaaataaa aaaacaatat acgcttttag
     7801 ccgtctgttt cttttgataa aaaacatcta tgctatacta taaagtgtaa catcatttag
     7861 aaaaaacggc gaattgcctg aacttataaa tttcaagcag aggaataagt aaaaaaggca
     7921 ggttgaccca ttaaaagagt aagcgggcaa tcaactgtga aaaggagcat ataaatatgg
     7981 tgtatcaatt ggcagaaggc gtgaatcttc atgtcttacc aacaaaacaa tacaaaacga
     8041 tccggatttt tatccgtttt actgcgcgtc tgcaacaaga agtcatcacg aaacgttcac
     8101 ttctcagcag catgttggaa acaaatagtt tgaattatcc ggatcagaca aagttaagtg
     8161 cgcaacttgc agagctttat ggcgcaagct ttggattatc agttagaaaa aaaggaaatt
     8221 tacattggct aaacgcaggg atcagttttg tgaatggtga atatgtcaat gatccaaacc
     8281 ttttttctca agcggtagat tttttaaaag aggttttatt ttatccaaat attaaaaatc
     8341 atcagtttga tcagttgacg tttgacttgg aaaagaacaa tctgcgtcta tatttggaaa
     8401 gtttgaagga agataaacaa acttttgctt cctatgcctt gcaagaactt tattttgaaa
     8461 acagcccgga acaaaagatc ccagggttgg gcgtagtcga agatttggac aagatcactg
     8521 ctagttcgct agcgtcctac tatcaagaaa tgatggcaaa tgatcaaatc gacatttttg
     8581 ttgtcggcga tgttgatccg gacaaagcag ctgaagcagt tgggcaatta ccatttgagt
     8641 cgcgggaaac agtttatcca gatctgtttt atacccaacc tcaagtgaat atcgtgaagg
     8701 aacgacaagt acgtgagcca atcgttcaag caaagctgaa cctagcttat cagacaaatg
     8761 tgtattacga tgaaccagaa cgttttgcgc taatggtctt taacggttta tttggcggtt
     8821 ttcctcactc taaacttttc atgaatgtca gagagaaaga aagtctagct tattatgcct
     8881 ctagtagcgt ggatacgttc cgaggattca tgagtgtcca aaccggtatc gatgaaaaga
     8941 accgaaatca agtacttcat ctgattcacg agcaattaga aagtttgcga aatggtgaaa
     9001 taacagagtt ggaactgtct cagacaaaag caatgctgag aaaccaatat ctgctttctc
     9061 tagactctcc gcaagcggca atcgaagctt cttttttgga cagctggctg ccggaaacaa
     9121 aattatctga tgaagaatgg ttgaaacgta tggagagcgt aacgatcaaa gagatacaac
     9181 aagtagctga gcaaatcgaa ttgcaggcaa tcttcttttt agcaggagga aatgcaaatg
     9241 aataaaacag aatatgagca aatcaatgaa acactttatc atgaagtact gccaaacggt
     9301 ttgaccgtgt atttgttgcc taaaaatgac tatcacaaga catatggttt gttcagtacg
     9361 aattatggtt cgatcgataa cgaatttatt ccctatggtg aaaaagaaaa ggtgaaagtt
     9421 ccagatggaa ttgcccattt tttagaacat aagttgtttg aaaaagaaga tggtgatgtc
     9481 tttcaattat ttgggaaaca aggagcttcc gccaatgctt tcacaagttt tacgaaaacc
     9541 agctatctgt tttctacgac ggatcaggta gaaaagaatc tgacgactct gatcgatttt
     9601 gtacaagcac cttacttcac agaagaaact gtaaacaagg aaaaaggaat catcggtcag
     9661 gaaatccaaa tgtatgaaga cgaccctaac tggcggatgt tttttggcat actaaataac
     9721 ttgtatccta cacacccatt gcatatcgat attgcgggca cggttgaaag tatcgacaaa
     9781 atcacagctc aagacttgta tacgtgctat cggacgtttt atcagccaag caatatggtt
     9841 ttatttgtag ttggtaagat ggagcctgag aaattgatga aacttattcg ggaaaaccaa
     9901 gaagcaaaaa atttccctcc aaagcaagaa attgtacgtt atttccctga aaacacaaaa
     9961 gaaatcatca aacaaagtgc attggaagca gccattacac gagataaatt tgtacttggt
    10021 attaaaggat tagacacatt gccgcaagaa ggaacagaac tgttacgcta taaaacagca
    10081 atcaatctgt tgttccaaat gattttaggc aatacttctc gaaactatct agcgatgtac
    10141 aatcaaggaa ttatcgatga cagctttggt tttgaattca gcttggatcg tgaatttcac
    10201 tttgcggact tcagcggaga tacggacgaa ccagagaaag cagcagaaaa agtcaaagaa
    10261 atcattcttg gattcgctga tgatccggaa gtgtcagaaa caaatctcga tttgctgaag
    10321 aagaaaatgc ttggacaata tttccaatca ttaaattcga tcgaatatat tgccaatcaa
    10381 tttacgcaaa gtttatttgg cgatcgaact ttatttgatc ttcctgaaat catcgattcg
    10441 atccaaatga aagacgtact ggcagctggg gaagcattta taagtgaaga ggccttcagc
    10501 gagttttaca tgagaccaaa ataaaccagt attttatgag gaaaagtctt aaatttcgtt
    10561 ttagacttgt agtaggaacc tcctccaaaa tcagctttta tggtattctt agttgtgttg
    10621 gaaaaattag tgagaaaata aaacaaatca tgctctcttt taatcaactt atattaaaat
    10681 aaaatcaaac gaagtaatca aagaggttgt gacagaagtg gtcagcttca agaaataaga
    10741 aggaatttac gaaaattgct tctcaaattt ttgtgatatt tcggcttatt gccgaagaag
    10801 ctacttctgg aacaccgtgt accaagaggt tgtgaaaaag ctgccctgca tcaagtaata
    10861 agaacagcca agtaaaaaca gcttcttaat gtttttaact tggttggact tattaccgca
    10921 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga aatcagcgtt ttgacctgag
    10981 tattagagaa acatacggaa aaatatctcc ttatattttg ccggtttttg agctaatacc
    11041 gaggaacagc tgattgaaca ccgtttaaga aagaggtcgt gagcctgccg tttaactccg
    11101 agtcacaagg aacaagttca aaaaacagct tcttatattt attggatatt gtgacttgtg
    11161 accgaggagt tggcaggaga acaatgttta ttcggaaaaa aggagctgtg acatgacttt
    11221 tgtcacagca cccaaacata acgtctttcg ttgcaacagc agatgcttcg gagaatcaaa
    11281 cagactggag aagatgaacg agtggctttt gaaaccatcg gagaaaaatt acggcaagca
    11341 cgcctgaata aaaaaatatc tttagatgaa ttacagcaga ttacaaaaat tcaaaaacgt
    11401 tatttggaag caatcgacaa tgatgatttt gaccaattgc ctggtaaatt ttacgtgcgg
    11461 gcatttattc ggcaatacgc tgaagctgta ggagaagatg gagaccattt agtcgatgtt
    11521 tttgatggga aaagcaaatt gacaggatcg gtccctgaac gtccgcagcc tgaaacagta
    11581 agtacttcta gaaaagcaat gcatcaggaa gaatcgcatc caagtcgctt ttggacaagt
    11641 cttcctgtca ttttattagg attggttgct ttggcgattg tcgttgtcgt gggctatatg
    11701 acttggcaag atcgtcaagc tgatccaata atcggagaca cagcttcttc gcttaaagta
    11761 gaaggatcag tcgaggagtc ctcttcctca gagcaaagca cgcagaccaa agagtcttct
    11821 acagtagaat caacaacaaa atcaagtgaa gaaaagaaaa tggcaatagc tacaggagaa
    11881 gattctggca gtgccatcgc agtaaatatc acagacgcta ccaagcctgt aacccttgaa
    11941 tttactgctg tcaatcgtgt atggttaggt gtgctagtag acaatgccta tgtttatcaa
    12001 gggacactgg cagcaaatga aacacagagt accgtgttac cggaaacagc aacaaatgcg
    12061 accatcacga ttggtgcagc aagcaatgca acgatcaaag cgaatggaga atccgtacca
    12121 gttaatccag gggaaaacaa tcagagtcca aaaaatgtga accttactct tcaatacgca
    12181 gaatgatcgt ataaattagt ttacgcatct gtttacttgt aggtataaaa aagaaggatc
    12241 gtgacaaatc tgtgtcgcgg tccttcttcg ttaattcaaa aagatcaatt tcaataaaaa
    12301 tactcctttg aataaattca tttggaaaag taaataatat cggtggaaac ttcgctgata
    12361 gtcacgtaga atagggaaga aaggagtcga tagtgtgaat ttaccaaata aattaactgt
    12421 acttagaatt tgtatgatcc cgattttcat tttggttgta ggtttacctt tagattgggg
    12481 acaatttaac gtaggagaca ccacactggc tgtcacacag ctagtaggtg cagttatttt
    12541 tgctttagct agtatcacag actggttaga tggaaaaatt gcccgtgcgc gaggccttgt
    12601 cactaatttt ggcaagtttg cggacccgtt ggcagataaa atgctagtta tgactgcttt
    12661 tatcatgttg gttgaccaag gaaaggcccc tggttgggta gtagcgatca ttgtctgtcg
    12721 tgaattagcc gtcacaggct tgcgtttact acttgtcgaa catggagaag tcatggctgc
    12781 agcttggccg ggaaaagtca agacggctac acaaatggtt gctattatct tgctgtttat
    12841 caataatatc ccattcagcc tgatcggtat tccagtagat caaatcatgc tttatgcttg
    12901 tctgatcttt actatctatt caggtgtgga ttacttcatc aaaaacagcg gtgtgttcaa
    12961 aggatcaatg taagtatcgt ttagtggaga ggttgtgagc ctgccgtcta actctgagtc
    13021 acaagggata atttcaaaaa acagcttttc a
//
LOCUS       DACESX010000074        12975 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.074, whole genome shotgun sequence.
ACCESSION   DACESX010000074 DACESX010000000
VERSION     DACESX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.074"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            137..1837
                     /locus_tag="IXF88_001774"
     CDS             137..1837
                     /locus_tag="IXF88_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1508504.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1807..2025
                     /locus_tag="IXF88_001775"
     CDS             1807..2025
                     /locus_tag="IXF88_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508505.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2045..2935
                     /locus_tag="IXF88_001776"
     CDS             2045..2935
                     /locus_tag="IXF88_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1508506.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2953..3237
                     /locus_tag="IXF88_001777"
     CDS             2953..3237
                     /locus_tag="IXF88_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508507.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            3724..4338
                     /gene="gmk"
                     /locus_tag="IXF88_001778"
     CDS             3724..4338
                     /gene="gmk"
                     /locus_tag="IXF88_001778"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1508508.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4343..4648
                     /locus_tag="IXF88_001779"
     CDS             4343..4648
                     /locus_tag="IXF88_001779"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1508509.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            4901..7309
                     /gene="priA"
                     /locus_tag="IXF88_001780"
     CDS             4901..7309
                     /gene="priA"
                     /locus_tag="IXF88_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1508510.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7324..7815
                     /gene="def"
                     /locus_tag="IXF88_001781"
     CDS             7324..7815
                     /gene="def"
                     /locus_tag="IXF88_001781"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1508511.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            7808..8746
                     /locus_tag="IXF88_001782"
     CDS             7808..8746
                     /locus_tag="IXF88_001782"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1508512.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            8746..10104
                     /gene="rsmB"
                     /locus_tag="IXF88_001783"
     CDS             8746..10104
                     /gene="rsmB"
                     /locus_tag="IXF88_001783"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1508513.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQRSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10117..10857
                     /locus_tag="IXF88_001784"
     CDS             10117..10857
                     /locus_tag="IXF88_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1508514.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            10854..12923
                     /gene="pknB"
                     /locus_tag="IXF88_001785"
     CDS             10854..12923
                     /gene="pknB"
                     /locus_tag="IXF88_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1508515.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
BASE COUNT         4403 a         2330 c         2857 g         3385 t
ORIGIN      
        1 taatttttgt gatttttgta ttttttattt aaaggatgtt ttatgaattg ttttcttatg
       61 gaatctacaa agtcgaatac gagactcatg acctttttat gacgtactgg tatacttgaa
      121 gaaaaaggag agcgcgatgg agataaaaaa agtttacttt tcacagaaaa tgtcgctgta
      181 ttcgcctatc cagcaagcaa aacaacccct tccatttttt caaagtcatc aagaaagtag
      241 agacgctttt tggcgttctg caaaaaagca atgtgctttt aaagcagtat cgaaaaaata
      301 taccgtctat tttactattg ctaaaagtag aggagatgtt atttttaata atctgttgat
      361 tgaaggtgaa acttttgaat ggaaaaaatt ttttcggtat atggaagctt gcgctcgttt
      421 ttttattaaa gaaaactgtt gttttttatt tcaatctcct ctatcagaag aatggttgcg
      481 tatttttcat aaaaacggct atcaaggaac cacacaatta aataaaaagc tggtctatca
      541 tacagcactt gttttaggtg gaggaggagc acacggtgct tatcagattg gtgtgtggca
      601 ggcgctgaag gaacatgata tcaactttga gattatcaca ggtacctcgg taggtgcgtt
      661 gaacggcgca ttgattttac aaggagatat gaaaaaagct gtgaatttat ggaaaaagct
      721 gtcgacaaga caagtgcttg cgttaccaga aatggctgca gttgaagacc ttcgtgaacg
      781 cttctatcga gaaagacgcc aaatgacgaa aacagcacta attgaaggcg gggtttcttc
      841 tgctccattg gaaaagatgg tcaaagacaa tttggatttg tccttactca aacataatcc
      901 caaaatacgt ttatatacgg tgtctactaa actgccagaa ctagcggaag tagtgactga
      961 tatccaacag acaaaaacag aagaaatacc agattggata ctcgcctctg cttcatttta
     1021 tcccgctatg gcttatcgaa agatcggaaa aaataaatat gcagacggtg ggtatcgcaa
     1081 caaaatacct attgatattg cgattaacaa aggagcaaca gaggcatttg tcgtagatgt
     1141 gcaagggcca ggcccagcga aaaaaattcg tatgccagac atttttatcc attggaaatg
     1201 tcaaacattg tggacactag gcagctttct gctttttgat agccaaagga accaattaaa
     1261 tttacaatta ggctatctgg aaatgaaaaa acgattaggc tattactatg ggaactggta
     1321 tacatttcat tctgttaaac ctgcaagaac atactggcga ggatttttgt cttacttaac
     1381 aaaagaaatt cagttagaat tatctttttt caaaagcata aaattttggc aaaaactgcg
     1441 taatttatat aagaacaggg tcgtaccaga gacatgcgga ttggcgatgc tagaactttt
     1501 agcaaaaaaa cattttttac tgcccaatga aatctatcaa gtagacatga tgattcaaat
     1561 gatctgccaa caagatataa aaagtatgcc ggatgattta ttggcacaaa ttggtcaatt
     1621 aagtactgaa gaatggcgaa ggtatcgaaa ataccagcaa aaaatacaaa atgagcgcac
     1681 gaaaactgtt tattttgatt atctgctaca aggaaaacgc aaggatcgtt tgcagcatga
     1741 attgcttaag caaccagttg acacattact gatcctatat ttatattatc tcaaggagga
     1801 acaaccatgg cacaagaatt ttcatatgaa atcgtagaag aaatcgctat attgtcagaa
     1861 aacaacaagg ggtggcgtaa agaactgaac ctagtcagct ggaatggtcg cccgccaaaa
     1921 ttcgatcttc gagattgggc gccagaccat gaaaaaatgg ggaaaggcct gacattgacg
     1981 aacgaagagt ttgaacaact gcaaaaagca atagaaaata tgtaatgagg aaggaccctg
     2041 tcgaatgaaa agtatgactg gttttggcaa agccatccgc gaaacaaaag cgtatcagtt
     2101 agatattgaa ataaaaagtg tcaaccagcg ttttctagat gttcaaatac gaagtcctaa
     2161 aatcctgaac tttgttgaaa acgattttcg tcagcttatt aaacagcagt taagccgcgg
     2221 tcgaatcgaa gtatttgtga atctagctta tattggcaaa aacgaaaaac agatcgtcat
     2281 caactgggaa ttgattgatt cacttatcac tcaattgaca gacggaattc aacaacgata
     2341 cggtagtcaa aatcaagtgt caattagtcg gatggcggaa cgcttcgcgc tggacgaaaa
     2401 cttcgtaacg gtagaagaaa aaccggaaga gagcatggaa gaattggcac agttagctct
     2461 agatacattg aaaagcgcac ttgattctat tgaggcgagt cgagaaaaag aaggtcaagc
     2521 tttagcttta gtgttgaaaa agaatactga agaattcaaa gaagtactaa aaaaactcga
     2581 tagttttgta gaaatctatg aaaaggaatt tcaaataagg tatcaaaaaa agctggagga
     2641 atttcttgga gcgaaagtcg atgagcaacg gttgttgacg gagctagcca tcttgttgga
     2701 acgcggagat attcacgagg aactggatcg tctggcgatc catgtacaaa aactagatga
     2761 acttatcgaa gcacaatgtc ctgtaggtcg ggaattagat ttcctgatcc aagaaatgaa
     2821 tcgggaaatc aacacgatcg gttccaaatc aaatgcaatc gaaattaaaa atcaagtcgt
     2881 ccagtctaag acgatcttag aaaaaatcag agaacaagta caaaatattg aatgacgaaa
     2941 gaggtagaga taatgaaata ttatgacgta acgttccacg aattaagtgg aaaaactgtg
     3001 gttaaacggg atatcccttc tgaaaaaaac ggctttgacg tatggaaaga tgcctgtgca
     3061 gactataatg agaacgaact gtttattttg atcaacgacg gtgcttacgt gacgatgaat
     3121 cggaaattta ttgttcgaat tgacacagaa gaggtagaag atccgacaga aaaagcacgt
     3181 tctcgaaaag atgagatcat gggtgtagtg aatactttgt ccaatatggg cttttagcaa
     3241 ggatgctgaa caaagaaata caataaataa tcagaattgg atttcttcgc ttagcttctt
     3301 tgctctagga atacatttaa aggttgtgac aaaattcgtg tcacaatctt tttttatttt
     3361 gaataagtga tgttcaaaaa ccattttctc ggtattaagc caaatttatg caaaaatgtg
     3421 agaaactatt ttcacaaaat ttttcttcat actcgaaact aatcggcttc ttcacgacct
     3481 cttatacacg gcgttcaaaa gcttgcatct gcgataataa ccccaaccaa acgaaaaaca
     3541 tgagaagctg tttatgtttg gctgttctta ttacttgatg caggacagct tttttacgac
     3601 ctcttggtac agggtagctt ttttaccaat ttctagctat aattaaagat aaccttgaaa
     3661 gttgcagaaa aaaagtgcaa aataaacgat aagtacaaaa agactagttg aaaggacgtc
     3721 gtcatgtcag agcgtggatt attaatcgtg ttgtctggtc catccggtgt aggaaaaggc
     3781 acagtacgaa aagcaatttt tgagagtgag gataatgatt ttcagtattc gatctcaatg
     3841 actacccgta aaatgcgtga aggggaggtc gaaggcgttg attatttctt ccgaagcaaa
     3901 gaagagtttg aatcaatgat cgaagctgga gagatgctgg aatacgcaga atatgtcggg
     3961 aattattatg gaactccttt gtcatatgtc aacaagacat tggatgaagg aaaagatgtt
     4021 ttcttggaga ttgaagtcca aggagcaaaa caagtcaaag aaaaagtgcc cgatggtgta
     4081 ttcatctttt tgactccgcc agatttagcg gaattaaaat cacggatcgt aggccgagga
     4141 acagatgcag atgaagtaat cgaagaacga atgaaagttg caaaagaaga aatcgaaatg
     4201 atggctcttt acgactatgc tgttgtaaat gatgaagtgc cgctagcagt tgaacgaatc
     4261 aaaaacatta ttgccagtga acattttcga gtagatcgcg taatcggtaa atatattaaa
     4321 atgttaaagg agatgtagtc ttatgatgtt aaaaccttca attgactcac tattagatcg
     4381 tgtaaattca aaatattctc tagtgatttt agcaagtaaa cgcgcgcatg aattggatgc
     4441 aagtgcacag cctactctag attcttttga ttcagtaaaa agcgtcggtc aagctttaga
     4501 agaaatcgat gcaggaaatg tagtcaatga tccacatcct gaattaaaac gtgaacgttt
     4561 gaagatggaa gaagaggaac gtcaagcaca aaaagagcgt gaacaacacg aattggaatc
     4621 acgtatccgt gaagagcaaa aaatgtaata aagaaaaata gagcgaacat ctaaaaagaa
     4681 aagcaaaccc agctgtttcc ttttcaaaca ttcgccactt attgtctggt agaaaaggct
     4741 gtgtctgaac tttttgacac agttcttttt tttcttatct gctgttaatt ttttgttagg
     4801 atttggcaga agataagaca aatccatcga tctctagtaa aataggcaag gaactttttg
     4861 tcgcttgata agaaaagaca aaaaaaggag gaaacgaacc atgacattaa cagctgaagt
     4921 cattgtggac gtacctacta tgcagaccga tcagccattc acttatttgg ttccctctga
     4981 aatggaaaca gctttgcaag tcgggatgcg agtagaagtc ccttttggta atggcaatcg
     5041 tcatgtccaa ggatttgtta tggctatcca aaaatcagaa gaatcagcta atccatcttt
     5101 gaaggcagtt attcgtttgc tagaccttgc tccggtggtc aatgaagaat tactttcatt
     5161 agctgattat atgaaaaaaa ttacttacgc attcaaaatc acttgtctac aaacgatgct
     5221 tccgagtgtg atgaaagcag aatatgataa attgatctat cctttggcag acacaccaga
     5281 agttcaggta ttgtttgatc agcgagaagt gatttcctgg aaggaagcag aagaagaagg
     5341 cagcctttct caattgattc gctggcgcca agagcaatta gtagatatca aatatgaagt
     5401 ccatacacga aataaagtaa aaacgattcg tttggtccgt tctttgttaa ctgaaaagca
     5461 aatcgaagaa gaatgggcaa aattacggca aaatgccaag aaacaaaaag aacttctttt
     5521 gtgtttatct gaactgagcc aagaagaacc aattgcttat ttcaaaaata aaggaatcag
     5581 tactgctgtc ttgaatcaag gaaaagaaaa gggctggctg gagtttatag aatcagaacg
     5641 ttatcgcgac ccttataaag accgagtatt tgaccaaacg acggcattgg aactgaatgc
     5701 agaacaaaaa aatgctgtcg aacaaatcat cacagcaggt cagcagcaaa aagatcaggt
     5761 ctttttgctg gagggaatca ctggcagcgg gaaaacagaa gtatatcttc aggcaatcgc
     5821 tgatgttttg tcagaaaata aaacggctat tatgttagtg cctgaaatcg cattgactcc
     5881 gcaaatggtc gagcgattca aaggacgctt cggagaatca gttgcggtct tacatagcgg
     5941 actttcccaa ggtgaaaaat atgatgaatg gcgcaagatt gagcgggaag aagcgcaagt
     6001 agtagtaggc gcacgatctg ctatttttgc cccgctgaaa aatatcgggc tgatcattat
     6061 cgatgaagaa catgaatcaa gttacaaaca agatgagaca ccgcgttatc atgcgagaga
     6121 tctagcaatt tggcgcagta aatatcatca ttgcccaata gttctaggaa gcgcgacgcc
     6181 ctctttagaa tcaagagcac gagctcaaaa aggcgtttat cagttattgc agttaaacca
     6241 ccgagccaaa gcagctgcac agcttccagc tattgagatc gtagatatgc gtgaagagtt
     6301 tcagaatcat cggacctcta ctttttctgc taatttgcag gaaaaaatac aaaatcgatt
     6361 agacaaaaaa gaacaaactg tcctactgct caatcgtcga ggatattcgt cttttgtgat
     6421 gtgtagggac tgtggatttg ttcttccatg tcctaattgc gatatctctc ttacactgca
     6481 tatggataca cgttcaatgc gctgccatta ttgcggacat gaagaaccta ttcccaatcg
     6541 ctgtcctaat tgtggcggca ataaaatccg ctactatgga acaggtactc aaaaagtaga
     6601 agaggaactc cgagaacttt atccgcaagc taggattttg cgtatggatg tagataccac
     6661 tcgaagaaaa ggggcacacg aacagatttt acaaaaattc ggtgcaaaag aagcagatat
     6721 attattaggg acacaaatga ttgctaaagg tttggacttt ccagaagtga cattagttgg
     6781 ggtgttgaat gctgatacct ccctgaattt accggatttt cgttctagtg agcatacatt
     6841 ccaattgctt acccaagttt caggacgagc agggcgagcg gaaaaagcag gcgaagtcgt
     6901 gatccaaaca ttcaatcctc aacattacgc catagaattg gctaaaaaac aaaattatga
     6961 gcagttttat cagcaggaaa tgcatgtacg gcatcgtgga ggctatcctc cttattactt
     7021 tactgtgaaa ataaccgcca gccatcctga agaacaagta gcggcaaaga aaatctttca
     7081 gattgccaat cagctaaaag aagtgttaag ccctcaaagc ttgctattag ggccaacgcc
     7141 cagcatgatt ttgagagtga aaaaccgata ttattatcaa ctaatcatca aatataaaca
     7201 tgaaccgaat cttcctcatg ttttggatga aattttgaat ggaagccaga aagaacagcg
     7261 acaaggtctt tttgtggcaa ttgacaacga accattaaac tttatttaag aaagggccaa
     7321 attatgcgct atcctatatt gatccaccct aatgataaat taaaaagaac tgctcagcca
     7381 atcgatgtaa tcacggacga gacaatcgct ttattagaca atttatatga gacaatgatt
     7441 gcgaatgacg gaatagggat cgctgctcct caagtaggac aaaacaagag aattgctgtg
     7501 atcgaagtag atgaaggcga gaaatttgaa ctgattaacc cagaaatcat cgaagcaaaa
     7561 ggtgaaagcc ttgacgtaga aggttgtctc agtattccgc atgtctatgg aacggtcaag
     7621 cgagcagatg aagtgaccgt gcgttattat gatcgagacg gagaagagat agaagtgacg
     7681 gcttttggct atttagcccg tgccttccag catgaaatcg atcacttaga cggtatcttg
     7741 ttcattgaga aaatgataca gcagatcccg gaagaagaac ttgaagaata tatggaggaa
     7801 catcttgatg actaagctag tatttatggg cacacctgct ttttctgtgc cgattcttga
     7861 agggctgata gaatcaggtt acgagatcca ggcagtggta acacagcccg accgtccagt
     7921 tgggcggaaa cgagtaatta cgccgacacc ggtaaaagaa gcggctctga agcacggtat
     7981 ccgtgtgctg cagccagaga aaatctccgg ttcgccagaa atggaagaaa tcattgaatt
     8041 ggtgcctgat gtgatcgtca ctgcggcatt tggccaattc ttgcctgaaa agttattgca
     8101 agtaccaaaa ttaggggcaa tcaatgtcca tgcttcttta cttcctaaat atcggggagg
     8161 tgcgcctgtt cattatgcca tcatgaatgg ggagaaagaa actggtgtaa cgatcatgga
     8221 aatgatcaaa aaaatggatg caggcggaat ctatgcgcaa gaaagcatac caatcactaa
     8281 acaagatgat gtagggacga tgtttgaaaa gcttagcctt ttaggaagaa agctattgct
     8341 agaaacattg cccaatattt tggatggaca aaaaccagta cctcaagacg agtcagaagc
     8401 aactttttcg ccaaacatca cacgggaaca agaagcaatc gattggcata aaactgcaga
     8461 agaaattgac aaccaagtac gcggaatgcg tccttggcca attgcattca cgacctatga
     8521 gggcatacgc tggaaattat tatcggttga agtattggca gaaactacag acaaagcacc
     8581 tggtacgatc atccggaaag acaaaaaaaa tctatggatt gcttgcggta aacagaccgt
     8641 cttagccatc aatgaattac agccagctgg taaaggaaaa caggccgtac atgatttcct
     8701 taacggaagc ggtcagcaag ttcagattgg tcagcaggtg aaataatgaa aaaaatccct
     8761 aaaaaaatga aaaaatcagt acgatatatg gcattactga cattagagcg agtgaacaag
     8821 ggtggcgcct actcgaatct tttgttgaat gagatgatca aacaaagcga gctaaatcaa
     8881 aaagatgtgg gtttatttac agaattgatc tatggtacga ttagccatca gcgactattg
     8941 gaattttacg ttgcaccatt gatcgctaaa gcaaaaaaag tggatgactg ggtcaagacg
     9001 cttctttatc tttctgtata ccaattagaa tttttagata aagtccctgc acatgctatt
     9061 ttaaacgagg cggtcgaaat cgcaaaagta aaaggaaatc ctgggacagg gaaatttgta
     9121 aatggtgtct tgaggaatta tcagcgtcaa ggagcaccag atctgcaagg cattgctgat
     9181 cctatcgaaa gactttctgt caaaatcagc ctgcctctat ggatgactaa acgactggtg
     9241 gatcaaatcg gatacgaaga aactgaaaag ctcggtttat cgctatatca gcgtagccat
     9301 gctagtgccc gagtagatac acgccgactt tccagagatc aggccatcac tgttttgcaa
     9361 gaagaagaga tcacagctga aaaaagccaa gtcagtccat acggtatagt agcgaaaaaa
     9421 gggcatttag cttctagctc attgttccac gatggcgtca tgacgatcca agatgaaagt
     9481 tcgatgcttg tagcgccgtc tatgcaaatc gagcctgaac atcatgtctt agatgcctgt
     9541 gcagcacccg gaggtaagac gacgcatatt gcttcttttt tagatccgtc atgcggtgga
     9601 gaagtggtcg ctttagatgt acacgaacac aaagtcaaac tcattgaaga taatgcagca
     9661 cgtctaggtg tctcttctgc tgtctacgct caaaagatgg atgctagaga agtcagtaaa
     9721 cagttcgaag cagagcagtt tgatcggatt ttagtagacg cgccttgttc aggtcttggg
     9781 ctgcttcggc gaaaaccaga tattcgttat aaaaaacagc cggatgaatt agcgaattta
     9841 ccaaaaatcc aattggaaat tttagaaagc tgcgcaccaa ctttaaagtc ttccggtatt
     9901 ctgacttata gcacttgtac gatcttaaaa gaagaaaacc aagacgttgt ttctgctttc
     9961 ttggaaagac accctgattt tgagaggatt gatgttttga ccaatcctat gctacaatca
    10021 tcaatcgaag agaaaatgtt gactttgtat ccgcatcagt tttacacaga tggatttttc
    10081 atttgttgtt tgcgcaaaaa gtaatgaggt gaaaaaatgc agattgaata tcaatcagat
    10141 gtaggaagac gacgtaacac aaatcaagac tatgccagtg tatttaccag ccgttcaggt
    10201 ctcaagcttg ctgtattagc agacgggatg ggtggtcatc gtgccggaga cattgccagt
    10261 cagatggcgg taacgaatct aggcgaagct tgggaaaaac aagaagctgt tgacgatgaa
    10321 aaaatcgccc aatggttcat tcaatcgatt caagaagaaa atatgctgat ttatcagcgt
    10381 ggacaagaac agccggaata taacggtatg ggaacaacaa tcgtagcggc cgctctgtca
    10441 gatgactggt tcacgattgc caatgttggg gatagcagag cttatttgat tcgtgaagat
    10501 aaaatcatgc aattgacgga ggatcattct ttagtcaatg aactagtcaa atcaggagaa
    10561 atcagcgaag aaatggcagt gaaccatcca aggaaaaata ttttaacacg ttctgtcggc
    10621 atgcctggaa cagtcgaagt agatgtttct actcatcttt ggcaactaaa agatcgactg
    10681 ctgttatgtt cagatggctt gacgaacatg ctgtctgaag aagaaatagg aacgatcatc
    10741 aatcaagaag gcccattgtc tcataaagta actgagctga ttgatcgtgc caatgaagca
    10801 ggcggagcgg ataatatcac cgttcttctt atcgaatata aggaggacag tgtatgatcg
    10861 agttgggcaa aaaattaaat ggccgttatc aaatcactgg aaatatcggt agcggtggta
    10921 tggcaaatgt atttttggcg cacgatttaa tcttggatcg tgatgtagca gttaaagtct
    10981 tacgatttga ctttcaaaat gaccagactg cgattcgacg atttcagaga gaagctttag
    11041 cagcaacaga actcgttcat ccaaacattg ttagtgttta cgatgttggt gaagaagaca
    11101 acatgcagta tctggtgatg gaatacgtca aaggaatgga cttgaaacga tatatccaaa
    11161 cacattatcc tgttccttat gaaacagcag taaatattat gcagcagatc ctttctgcta
    11221 tttctttagc tcataaccat caaatcatcc accgagattt gaaaccccaa aacgtactga
    11281 tcgataatga aggtgttgta aaaatcacgg atttcgggat tgctatcgct ttatctgaaa
    11341 catcgatcac gcagacgaat acgatgctag gatctgttca ttatctgtcc cctgagcagg
    11401 ccagaggaag tatggcaacg aagcagtcag atatttatgc tttagggatc atcctttatg
    11461 agatgttgac gggtagcgta ccgtttgatg gtgaatctgc tgtaacaatt gctttaaaac
    11521 attttcaaga tgatcttcca tctataaaag cacttgatcc aaatgtgcca caagcactgg
    11581 aaaacgtgat tttacgggca acggctaaag aacctgcaga ccgttataag tcagccgagg
    11641 aaatgtctga tgatctttct acagtattat caccagctcg tgcaaacgaa gaaaaatggc
    11701 agcctcatgt gatggataat gaaacaaaag ttatcacacc ccttacggaa gatgcaccaa
    11761 tgcctgattc attcaaatct atgcctctgc caaaagacaa gccagaaaac gaaaccttac
    11821 cagaggagga agaagttctg cctgatccta aaaagaaagg acagaaaaaa tggtggctga
    11881 tcctattagc tattcttgct gttttaggaa tcggaacagc tgtattcttt gcttctggcg
    11941 ggcgaggaga agttgttgtt cctgatgttt ctgatctttc tgaagcttct gctagagaaa
    12001 cactcgcaaa agcgaaatta gaagtagcaa caaaaacaga agaaattgca gatgacaaaa
    12061 tcgaagaagg taatgtagtg aagacagacc cggcagcggg aacgacagtc aagcaaaaac
    12121 gcgaagtcac tctgtatatt agtagcggca cgaaaaaaat caaattagat gattacagtg
    12181 gtaaaagtta tgaagatgcc gaagcaagtt tgaaagaatt agggttttct gctgacttga
    12241 ttcaaaaaga agaggaattc agttcagagg tagaaagcgg aatgatcatt tcgcaatctc
    12301 catcagaaga tacagaagtc gatcctaaaa cagacacgat tactttcaca gtcagcaaag
    12361 gaccagaaac agtgactttg agtagtcatg taggagaaaa ctacgaaact gttcgtaatt
    12421 ggcttgttac acaaggattc aatatcaata atattagtgc tacttatgac tatagcgaca
    12481 ctgtaccatc aggttcgatc atcagtcagg atccagcctc gggtgaagtt gtagcagaag
    12541 aaacttacat caattttgtc gtaagccaag gaacagagcc taaattgaat gatatctccg
    12601 gctatacaaa aagcgaagca caaagttatt tagctagtgt cggtgcagaa tatatcgggc
    12661 atgagacttc cgaatattca aacacggtgg ataaagataa agtgattcgt acagtaccag
    12721 gggcaggaac gacaattacc aaaggaatgg ttgtgaacgt aatttactct aaaggaccag
    12781 atccagcgtc ttctagttct gaaaaagact cttcagacag ttctagcagc tcaagtagct
    12841 ccagtagcac aagcaactct tcaaaagaag aatcttccag ctccacaagt tcatcagcta
    12901 gcacctcaga aagttcaaaa taagtgctga ataaaatata tcagaaggga atgcgacatt
    12961 gatttgttgc attct
//
LOCUS       DACESX010000075        12907 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.075, whole genome shotgun sequence.
ACCESSION   DACESX010000075 DACESX010000000
VERSION     DACESX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.075"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            127..3654
                     /locus_tag="IXF88_001786"
     CDS             127..3654
                     /locus_tag="IXF88_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1508516.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="IXF88_001787"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="IXF88_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1508517.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="IXF88_001788"
     CDS             7442..7783
                     /locus_tag="IXF88_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1508518.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="IXF88_001789"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="IXF88_001789"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1508519.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="IXF88_001790"
     CDS             9140..11560
                     /locus_tag="IXF88_001790"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1508520.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IXF88_001791"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IXF88_001791"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1508521.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4384 a         2243 c         2738 g         3542 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgtgcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaacttt
//
LOCUS       DACESX010000076        12901 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.076, whole genome shotgun sequence.
ACCESSION   DACESX010000076 DACESX010000000
VERSION     DACESX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.076"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(104..787)
                     /locus_tag="IXF88_001792"
     CDS             complement(104..787)
                     /locus_tag="IXF88_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1508522.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(805..1422)
                     /locus_tag="IXF88_001793"
     CDS             complement(805..1422)
                     /locus_tag="IXF88_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1508523.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(1432..2256)
                     /locus_tag="IXF88_001794"
     CDS             complement(1432..2256)
                     /locus_tag="IXF88_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1508524.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(2249..2809)
                     /locus_tag="IXF88_001795"
     CDS             complement(2249..2809)
                     /locus_tag="IXF88_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508525.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            2983..3939
                     /locus_tag="IXF88_001796"
     CDS             2983..3939
                     /locus_tag="IXF88_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1508526.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(4244..5200)
                     /locus_tag="IXF88_001797"
     CDS             complement(4244..5200)
                     /locus_tag="IXF88_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1508527.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            5304..5906
                     /locus_tag="IXF88_001798"
     CDS             5304..5906
                     /locus_tag="IXF88_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1508528.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(5994..7493)
                     /locus_tag="IXF88_001799"
     CDS             complement(5994..7493)
                     /locus_tag="IXF88_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1508529.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(7509..8033)
                     /locus_tag="IXF88_001800"
     CDS             complement(7509..8033)
                     /locus_tag="IXF88_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508530.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            8331..9080
                     /locus_tag="IXF88_001801"
     CDS             8331..9080
                     /locus_tag="IXF88_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1508531.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9071..9856
                     /locus_tag="IXF88_001802"
     CDS             9071..9856
                     /locus_tag="IXF88_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1508532.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            9825..10541
                     /locus_tag="IXF88_001803"
     CDS             9825..10541
                     /locus_tag="IXF88_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1508533.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(10643..11662)
                     /locus_tag="IXF88_001804"
     CDS             complement(10643..11662)
                     /locus_tag="IXF88_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1508534.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(11681..12739)
                     /locus_tag="IXF88_001805"
     CDS             complement(11681..12739)
                     /locus_tag="IXF88_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1508535.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         3766 a         2669 c         2323 g         4143 t
ORIGIN      
        1 aaaaaaattt ctcttttttt tgaattctga acaaatagta ctggagtaca aaagacgagg
       61 aagagccact atctgctctt cctcgtcttt tgattctcac ctgttattca tgattctttt
      121 tctcagtttc ccgatatttt tccattttac gactaaaaag ctctccgcta cttggtggcg
      181 tatatgtgat ggcatttgct cctgcttgaa tggtctcagc aatactttct tccgttggac
      241 cacctgtcgc aatgatagga agttcaggat attttttacg aaaatggcgt actgcttctg
      301 cagtttcttt acctgcactg atattgataa tcgaaatacc agctgctaac ttttcatcta
      361 tttctgtata tttcgacgtt accgtactga taactggaat atcaactact cgacaaactt
      421 cttttactgt ctctaaaggc gtaggaccgt tcaaaacgac ccctaacgaa ccttgcgctt
      481 ctgcgaacat gctcatgtag gaagaacgag caccttgtgt caaaccacca cctacaccgg
      541 agaaaactgg aatgtcagct gcctcaataa tgcttttagt aatcgctgga tgaggtgtaa
      601 aaggatacac agcaattact gcattcgcat ttgtattacg tatgatcgca atatccgtag
      661 taaagatgat cgaacggatc aattttccaa atatcttgat cccgcttgct tctaaaatga
      721 tttccggtac tcttacgata tcttttctca attccgtcat gatttctggc gtaaattgtt
      781 ctgccatcag ccacttctcc tttgctatct ccggtcatac tgattcaatt gatacgtctc
      841 gatgacctgg atgatttttt gcgcataacc ggggtctgtc gcataacctg catcttgcaa
      901 tgcttgtgcg gcttcctgat agttggtggc cgtaatcact ttttcataca gcgcaggatt
      961 ccaatctact ccttggacga acagttgtgt atggtcatcc atggactgtt cccatgaatc
     1021 atacactttg aagtctcctt ggatagttat ccattcttcg ttaacaaatt cctttgtttc
     1081 caggcttact ttcttctgat ccccataagc cttgatcccg aaaagattat tgtatttgct
     1141 agccaaggta ctttttcccc aatttgattc taatatagct tgtcctaaaa tgatactagg
     1201 aagcactcca taaccatctt gtagttcttg ggcatgcgga acaagccgtt caataaattg
     1261 ctgatgggat tgcgcttcat tttcttgctg attatcagta aaaggagaag acagattctt
     1321 caaggaaaaa acaaaggcta agagtatcac tgctacaccg gcaaccactg ctggccaacg
     1381 gatattcttg cgcttttgct ttttccactt ttttttcgcc atgatcaatt cctacttttc
     1441 atttaattca ttcagatatt cttctagcgt caattggtgt ttggtgatat actcagcgtt
     1501 ttctttaccg acataacgta agtgccaagg ttcatatgtg atcttagtta tatcttcgtg
     1561 tccttttgga taacgaacga taaatccata ctgcgcagca ttatctgcga tccatttagc
     1621 tccaggctga tctccgtaac ttgcatccaa tacagtagaa ggataactgt tgtaccagtt
     1681 ttgatcgacg acatcgactg ccagacctgt gtggtgttcg ctgtatcctg gctccgtaat
     1741 cgtttcttta gtaatctttg tcgcctcctc ttctgataca ccttgacgac tcatcacatc
     1801 ttggacattt tgttcgaaaa cttgttgttg ggatgataca gaccggtaag ctgagaccat
     1861 cactaatgga aatccagctg cttcagcagc cttggcaaat gcttcatatt ctggagcaat
     1921 gcgtttgtcg atcaagtatc cgttacttaa tgatgccaat tgagtagact catcaatttc
     1981 tttttctaat ttgtgatctg gaccaaccaa aactagcttc caatcgtctg ttttaacatc
     2041 tggcaattgt ttggacgtac gatcttttgt ttctttgtcc tttaccttgc tgctggttgt
     2101 ttgactgttc ttttctttac tagcagttgt tttggtgctt gttcgttcga ctgcttgatt
     2161 gccggcttga acatgcgaat catcatcgct aaaaataaca tagcccattg ctgcgatcat
     2221 aatcacaact gctccaaatc ctaaaaattt attcacttga aaaatccttt cgttgttctt
     2281 cacgttcccc attcgcctca tgaacgtaag tttcttccag attttcattc acccaatcga
     2341 gaagttcgat tttttctcct tcgatctgtt tttgaacatc tgctggcaga cgaaaattca
     2401 aaatatcatc atagccccat tcatcatatt gaatacttac tttcaagtca aggtatctac
     2461 cagcctgctg attatttttc aaagggatca gctcgacttt tgctgctttg ctgctaagcc
     2521 atttttgcgc gatgcgtgtt ttgatttgct tgtactctgc aacggcaacg tttcgttttt
     2581 cgggaaaaat aatcgtttga cgcaaaatct tttgcgttag catccattca taatcagtga
     2641 aaagattttt tactttctgc atcgcttcag atggcagatt tttctcgcca tctttccatt
     2701 tttgccattg ttctggactg atcagcaagt attcatctaa aaaatttttt tcagtttcaa
     2761 actgttctaa taatacactc ataataagat caatatatac ttgatgcacg tttatcccct
     2821 ccttttaact attctactta agttttgatc aaaaagcacc tcttaaatcg ctgctttcat
     2881 tttttttaat gtttcaagtc ctctcctctt tattcttcgt gatttttgtt ataataatag
     2941 acagacaaga aactcaatcc aactatgaag gagcgttttc agatgaaaaa atatcaatgg
     3001 ggaatcgttg gtcttggtac catcgcacat gagtttgccg aaagcttcaa tcaagagacg
     3061 agcgaactaa cagcagttgc ttccagaact tcagaaaaag cggaaaactt cgctcaccgt
     3121 tacaatattc caaaagctta cggaagctat caagaaatgt tagacgatgc tgaaatcgac
     3181 atcgtgtaca ttgctgtacc aaatagacag catatcgacc atatcttggc agcattggaa
     3241 gcaggcaaac acgttctatg tgaaaaagcc atcacgatga ataaaaaaga actggctgac
     3301 gctatgcgct tagcagaaga aaagaatttg atcttagcag aagcgatgac aatcttcaac
     3361 atgccacttt atcagcagtt acgctcaata atggatacag gaaaactggg ggctttaaaa
     3421 atgatccaag cacctttcgg cagttacaaa gaaccagacc ctaaaaaccg gttcttcaat
     3481 ccagaattag ctggaggagc tctgttggat atcggtacgt atgctgtatc tttcgctcgt
     3541 ttctttttga gctctcaacc tgaagtagtt gcctctacaa tggttccttt tgaaacaggc
     3601 gtggacgaac aatccgtcac tatcttgcgt aacaaagaaa atgaacttgc tgctgtcagc
     3661 ttgacttttc aggcaaaaat gccaaaagtc ggtatcgttg cctttgagaa cggttatgtg
     3721 acgattgctg attacccgcg tgctgatcgt gcggagatct tatttacaga tggtacaaag
     3781 gaattcatcg aaagcgggaa tacatctgaa gcaatgaatt acgaaataaa gaatatggta
     3841 cacatgattg aaggtgactt gcccaatcgc tctctcttct tgacaaaaga tgtcatcgaa
     3901 atcttggatc aaatgcaagt tctatggaaa aatatgtaat tttaaaaggt tgtgaaaaag
     3961 ctgttctgca tcaagtaata agaacagcca aacaaaaata gctcctccta tttttcgttt
     4021 ggctggactt attaccgcag aggaaagctt ttgaacaccg tttaagaaag aggttgtgag
     4081 cctaccgttc agctccgagt cacaaggagc aatttcaaaa aacagcttct catgtttgtt
     4141 gaatattgtg acttgtgacc gaggagttga caggagaaca ccgtttaaag aagaggttgt
     4201 gaaatcagct ttttgacctg aatattagct gaattcacaa cctctatttt tggatttttt
     4261 gttgggagaa ttctgccttg atttcaataa aacttttaaa agactgcaaa aactggaaca
     4321 attctgctcg atcctcgaat tcagggcgac tagtcggcag aggcatttgc cgatacaata
     4381 cataaacttc ttcaatacgt gttaaaattt gtctgccatc atttgactct gaaaatgttt
     4441 ctgcagtata aatcaataaa ccatagatat gttttccata ataagctggt tgctggattc
     4501 tctctagatt ttccagcatg tctttaatca cattggcctg tgctcttctc atagagaagt
     4561 aagtttcgta gtatagcggc tgtctgagcc agtagttttc ttgatgttct ctggcatctt
     4621 cttggctttc gcgaatgaat gtcagtaaat gttcgcattt gatctgcaca tcttctttat
     4681 tttctgataa aagaaattct gccatttgtc tcaaaatatt tcgaaattct ttttcgatct
     4741 gtatttggtt ttccttcagc tgcttcgtac gatctggcat gtaaacattt gcaagcaaag
     4801 caaatactaa tccaatcgcc attaatcccg cttcattgcc gatcagctgc caagacatgt
     4861 tttcctccac taaataatga gtgaccaaaa ccgaatttac tactatccct tcggttaatt
     4921 tgaagcgaga ggcaagcggg atgaacagga ataaaaaaag actgaagcca aaaatagtat
     4981 acccaacagc aaaaaaactg aacagtgctg ttgccgtagc taatataaag gctgcgattc
     5041 gattcatgcc cgtacgcaat gtagagcgct tggtatttcc tacacttaat aatgcaatga
     5101 tccctgcagc tggccaatac aacagcgata agctacccgc tgtgaccatc gaaaagaaga
     5161 cactgatcac tgttttgact gttcgcattc ctatacgcat ttacttcccc cttttttatt
     5221 ttaattatac ttgaataatg aaaaactagc tatcagacaa aagtttaggt atgatagtaa
     5281 aaagaccaaa aaggagaatc tctatgcaat ttggccgttt tcgactaggt atgcgtacga
     5341 tcaaaagtgc cttagctgtt tttttatgta tcttgttttt tcatttgact caacgaggaa
     5401 tcccgatgat tgctgcatta tctgctgttt tttctttgcg tcaggacttg actacaaccg
     5461 tttcttttgg aaagtctagg atcatcggca atacattagg tggtacatta gctatcattt
     5521 atttttttgt caaaagctat tttcacaatg attttttggt agaattgatc ttgcttccta
     5581 ttctagttat tattgtgatc gtcgtgtcag atggtattaa caataattcc ggtatcattt
     5641 ccgcaatagc tacgttgttg ctgattgcgt tgagcatccc tcaaggcgaa tccgctttgt
     5701 atgctatcca acgtgtacta gatactttta tagggacatt cattgcaatc ggtatcaatt
     5761 tctttcttcg tcctcctgaa acagaaaaga aggaagaact atcagaagat ttggtcgaac
     5821 tccaaaagaa agaacgattg ctaagaacaa atttacaaga gatagaagct aagatccaaa
     5881 aacaaaagaa aaatagcgag gactaatttt cttttgcaat aaaataaaaa aatggagaga
     5941 agaactttaa aaagtcttcc tccatttttg attgaacggt gaagtgtact actttattct
     6001 tccttcatcg agttttgttc tgcctctttt ggcgcttcat acatatttac ttggctagta
     6061 gtcccacgtg tcgacaaatt ctctacgatt tccttcaagt caagtcctgt tgtcgctttt
     6121 agtgtttctt gagtagaaga aagcaaattt gtggcataat ttgtcactcg gttcgctcca
     6181 ctggtttctc ctgaactgct tcctgtatcg acaacagaga ttttttcgat atttcctagc
     6241 ggctgtgctg cttcgcgcat caattgcggt agcatatcga taaccatact caagacagct
     6301 gcttcgccat attccttgaa ggcattggcg atcttttgtt ttgcttcggc ttctgctttt
     6361 ccttttgcta gtgctgcttc ggcttctgct tgtccagcta aacgtgtctt attggcttcc
     6421 gcttctgcca gtgcttccac acggaatcgt tctgcttcgg cttcagtcac ttctttgact
     6481 ttttgagctt gtgcttcttg ttctttagcg taccgatcgg catctgcttt tttcttcact
     6541 tccgaatcat attgcttttc acgacgtgtg atttcttttt cttccagttc gatttgtttt
     6601 tgacgttcta cgattttaat ctgcatttcc tgttcgatta cttgttgttg tgctcgggca
     6661 ctttctaaat tgtaggcttg gtctgctttt gctttagcaa catcttgttc ttgtttgtag
     6721 gttgctaatt tcagttcttt ttcttttaat gattctgcga tctctgtttg tcgctgtaat
     6781 tcagctgctt gtgattcttt ttctgcttga gcttttttga tacgcgtttc ttttagagct
     6841 tccgcttctg ctatatctgc atctcttttg acttgggcga ttcttggttt ccctaaagag
     6901 tcaagataac cgtttttgtc tctgacctct ttgatagtga aagaaacgat gatcaacccc
     6961 atttttgcaa ggtctacaga agcgacttct tgtacgcttt gactgaactt atcgcgattt
     7021 tgatagattt cttctacagt catagaacca agaatcgagc gcaagtgtcc ttctaatact
     7081 tctcttgctt cattttccag ttcttctctt gtcttgccta aaaactgttc tgctgctgta
     7141 gcaatctctt ctacagatga accgattttg atgatcgaag taccatcaca catcaccggt
     7201 acgccttgtt ccgtatacac ttcaggggtg gagacgtcca atttactcga taatagactg
     7261 atccgattcg aacgttgaaa aactggcaag acgaatgcgc cacctccacg cacaattttg
     7321 attttgttat tgctttcatc aatgtgcaca tttttgcttc ctaaataact tccactgatg
     7381 atcaacgctt catccggttt tgctgtctgg tacttcgcta caaagacgat cagcaacatc
     7441 agtaaaatca aagctgcgat tgcgatcgcg ataaaaacaa ctggtatttc catttaacac
     7501 acgctccttt aagcttttat catgctttca taaggcatga cataacagat tctttccttg
     7561 atctcaatga tcaatacttt cgttccccgc tcagctgtct gttcttgggg gctgtaaaaa
     7621 caagctggac gactaatgga accggtaaca cttttcaact gaatttctcc catacctcgt
     7681 acagggatcg gtgtgatcac aacagctact tgtccttcca gagttttttc agaagacgac
     7741 atcgttgatt cggcatgttt catcggcatc aatatataga aattaagcaa aaataccaac
     7801 actatcgata tgcctccact agcaataaat aaaaataaag atgattgatc tgttaaacgc
     7861 tcacctagaa aaccaaataa gcttgtgaaa gttaaccaag ggataataag catcggatca
     7921 agcgggccat caaaatcgaa tacatctcca aaaataatca ataggactgc caatcctgca
     7981 caaatcagca atgtatacca ataaatcgtc tcaagtgcga ttccccctat catatacacg
     8041 cctccttcca tttctacgtc tatcatacca atgtttgaaa attctgtata gcttctttta
     8101 ccgactcagt ggtgtttttt ataggaaggt tgtgtttatc gttgacaatt tgttaaattt
     8161 ttgttttcat gttaaaattt tcacaataaa ttctttcgag gtagcctatg tttccttttt
     8221 ttaagaaaaa accattaaag ctccagaaca cgatcatcct tgtcgtttgt ttctccacat
     8281 tcgtttcttt gattgtttct gccatattga tcaggaattt tattctttcg atgaaccgat
     8341 ctagtgtgtc agataaaatc tctagcattg ctaaaatcgt tgccaatgat aaactcatcc
     8401 aaaaaaagct gcagactggt gacccgacac atgcgattca aggaataaca aatgaaatca
     8461 tgattgcatc gaatttagat tttatcgttg tcatggaccc tgaaggcatt cgatattctc
     8521 acgcagaccc aagttacatc ggtcttcctt tctcaagtca tgaagatgtg aaaaaagtga
     8581 ttggaggaca agaatttttc tctgaaaaag aaggaatgtt agggttcggc ttccgctatt
     8641 tcgtccctat ttattatgat caagagatta ttggtgcagt ttgtgttggc ttaacaaaca
     8701 acacgattga caatcaagtc aaagatggac aaaaaatgat tgttttttca ttgattattg
     8761 gacttagcgt aggcatcata ggcgccgttt ttcttgccca aaaaatgaaa aaagtactgc
     8821 ttggacttga accacaagaa atagcagaac agttaagtga aaaaacgatt atcgaaaatg
     8881 aagtagatga aggaatctta gccatctctt ctgataaaac tatcctcttg atcaatacac
     8941 ctgcaaaaaa cattttctac caaacagagc agctattgga tttcgaagta ggaaaaaaat
     9001 cagtgataat ctatatgacg ttttgtttaa agagacgatt gagcatcaaa cgaccgttcg
     9061 gactcaatcc atgtatttaa atgggataga aactatttct agcgttacac ctctttttag
     9121 caatgaccgt tttttaggag cagtcgcaac tttacgaaat cgatcagaag taatgaaatt
     9181 aatggaggag cttgggggga cgaaacaata tataaactcc atgagagctc aaacccatga
     9241 attcatgaat aaacttcatg tcatttctgg tttgattgat ttgaagaagt atgaagaagt
     9301 cacccatttt atccagcagc taaatcaaaa ttataaaaaa gagattggcc aagtctcaag
     9361 tctgatcaaa gtacctgcaa ttgccggttt tttattaggc aaaataaacg aagccaaaga
     9421 acaatctgtt gaaatgctgt tagaaagcga ttcttttatc cctaaaatgg agatggatga
     9481 ctccatccat tcacttttgc aagtgttagg gaacttactt gataatgcaa aagaagccgt
     9541 gctacaaaaa catgaaagaa acggacaggt ccggattcat ttaaactatg acacagaagc
     9601 tgaagtattc atcgtgaccg tctgcgatga cggtgtcggg atatcagaag aaatctcaga
     9661 aaaaatattt acgctaggtt tttcgacgaa gggaatcaat cgtgggtttg gattgaatct
     9721 catccattca atcgtcaaac gccacggtgg atttttagaa ttttccaaac aaacaaatgg
     9781 cggtacaatc gtctatctag aattaccaaa aaaggaagat caaaatgatg acaaatgtcc
     9841 tgataatcga agatgatcca atggttgcag aattgaatcg tacttttgtg gaaagcatcc
     9901 ctaatatgaa cgtaatcgaa agtttcagaa atgtagaaga aggaaaaaaa tatttcagac
     9961 aaaaaaacgc agatctgctg ctgcttgatg tatacatgcc tggcgaaagt ggtatccatt
    10021 ttttatcttg gcttagacag caaggaaatg atatcccagt gattttgatc actgctgcag
    10081 atgatatcga aaccataaag aatgtgatcc gctacggtgc cttagattat ttgatcaaac
    10141 cgttcacttt gcaacgtttt cagctggcga tcgaaaagta tcttgctttc tctcagacaa
    10201 tccaaacgac tgaaaaagcc gatcagaaat tgatcgatca agtattcaac cacaccactc
    10261 ccattcacga aataccatca ttcatcgatt tgcctaaagg tctgtcaaaa ctgactttaa
    10321 aaaaaataag tgcatttatt ctaaaagaag atactatttt ttcaacaaaa gaactggctc
    10381 aaaaaagctc actgtcacgt atatccgtca agaaatattt gaatttttta gtagaacaag
    10441 atatattgaa cgaaagaatc aaatatttgg aaatcggccg gccgctaacc tgttatgaat
    10501 tgacaaagaa tgggcggaaa aagctcacag attactgttg agtcatcgaa ttatcccata
    10561 caaaatagtc caagtataca gaagtaacaa ctgtttactt ggactatttt taaacatttt
    10621 attgacgaat atccatgttt tcttatctta attgacgggc tttaggaaga gatacctcat
    10681 ttttcgtttt tccagtttct attatttcac gaaagttctc gaaacttgtc gaaatcatat
    10741 ttgataatgc ctcatctgta tttgaaccta catgcggggt tacaagaact ctagggtaca
    10801 tttctactaa ttgctggata gcagggtctg gaatcgtctc ccatggcttg aaggatcgga
    10861 aaaaaacctc tgtttcattt gcaaaaacat cggttgcaaa accagctaaa tgattgcttt
    10921 tcaaagctct taaaatagct tcattatctt gaagctctcc acgggctgta ttaatcaaga
    10981 tgctaccttt tttcatttga gctaaaaatc cttcattgat catttgatcg ttttcacctg
    11041 ggatatgagg tacatgaata ctgacaatat cgcttttggc taataactct tcaagcgagt
    11101 ggaacgtcaa tactttttta gcagcttctg tctgatgaat atcgtagccg atcactttgg
    11161 ctcctagtcc cttaaacaaa acagcttccg tcaaccctat cctgcctgtt cctatgatcc
    11221 ctactgtgca atttctgatt tcacgactaa acatttgctc atccactcga aaatctttga
    11281 aagatgttcg gatcgtcgta tacgcagtat ttctcaaaag catcatggca aaagtcaaag
    11341 ataactctgc tatcgcattt ggcgaatacg atggtacacg ggctactgtc ataccaaaat
    11401 cagctgccgc ttgtagatcg atatgattga ctcccacagt ccgtgtaaaa acataacgaa
    11461 tcccatattc ctgcattttt gcaagattgg tctcatctgc tatacaattt cccctcaaca
    11521 atacagcatc atggtccttt gctgacacga tattgtcatg tgtcaacagt tcttcataaa
    11581 gactgagatc ataatggtaa atattcaatt tttcaaaaaa atctatctcg ttcggacgaa
    11641 caccgtaaca tgcgattctc atactttctt cctccatttc ttatgaaaat aaacctatgc
    11701 catgaatcat ttgtaataca acgatccata caggtgtcag taccacagca aaaatagttg
    11761 acagtaagga tgcattggag gctaaaagtg cctccctatc aaaactgata gcataggctg
    11821 ctgccactgt tgcagctggt gtagccatca tgataacgat tgttgccagt gcttcaaaat
    11881 tgactggtag aatattcgtt gccgtcaaaa ctgctagaat cacgatattc aatgcaggta
    11941 caaataccac tttgactaga ctgtaatacc atgaagtttt ttcagatgca gcttttttga
    12001 agcttacttc acctaatgtt gcgccgattg ctagccacgc caatggtgaa cttaaaccag
    12061 ctaaataatc catcggctga aatagccacg gtgcagtctg gtcaattcgc aaaaacgcta
    12121 cttggcttat ctctccttct gcacctgtaa ctgacacttg tggcagatac ccttggaaca
    12181 gccaaatgaa aaggccggca aaagtagcga taatgattgg attgacgaac atcgctttga
    12241 tatttttagc ttccatcttc aagccactca tttttatgta tccataagaa tacaaaaaga
    12301 ttcggtagcc aatattgaaa attgaggaat acatcacgcc gacaggacca taaatcgcac
    12361 tgacgatagg cgttccgaaa aaggtcgtag aaccaaagat cgtcaggaca cgcaacgttg
    12421 tttgcttatc ccctttatag cttaagaaaa aaggttttga aactagaata agaataatat
    12481 aaataactaa tccccaaatc aagacattga ttccttgcat cagtaattct gtattgatat
    12541 cttgcatgaa agctcgaaat gccagtgctg gcaaagccac tgttaagacg actttcgata
    12601 gaatttttcc aacagattca gaaaaaatcc ccttttttct acaaacaaac cctaataata
    12661 taataaaaac tgttgaagtg attgcactga caatcgacat atctgtcaaa gtcgatcgaa
    12721 aaatgtcaaa taagttcatc tttctcctcc taattatgtt catttattca caataaaaaa
    12781 agcttttatt ttgctaatac gtattaacat ttttaatttt atctttacat tgttacttat
    12841 tgaacaatag gaaattaaag aaacttttga aacttatgta aagtgatttt tactagaaca
    12901 c
//
LOCUS       DACESX010000077        12831 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.077, whole genome shotgun sequence.
ACCESSION   DACESX010000077 DACESX010000000
VERSION     DACESX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.077"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(92..1276)
                     /locus_tag="IXF88_001806"
     CDS             complement(92..1276)
                     /locus_tag="IXF88_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1508536.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1301..2308)
                     /locus_tag="IXF88_001807"
     CDS             complement(1301..2308)
                     /locus_tag="IXF88_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1508537.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2480..2839)
                     /locus_tag="IXF88_001808"
     CDS             complement(2480..2839)
                     /locus_tag="IXF88_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508538.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(2811..3182)
                     /locus_tag="IXF88_001809"
     CDS             complement(2811..3182)
                     /locus_tag="IXF88_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1508539.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3199..3501)
                     /locus_tag="IXF88_001810"
     CDS             complement(3199..3501)
                     /locus_tag="IXF88_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508540.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(3518..3988)
                     /locus_tag="IXF88_001811"
     CDS             complement(3518..3988)
                     /locus_tag="IXF88_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1508541.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(3963..4241)
                     /locus_tag="IXF88_001812"
     CDS             complement(3963..4241)
                     /locus_tag="IXF88_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1508542.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4238..5287)
                     /locus_tag="IXF88_001813"
     CDS             complement(4238..5287)
                     /locus_tag="IXF88_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1508543.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5244..6173)
                     /gene="cpaF"
                     /locus_tag="IXF88_001814"
     CDS             complement(5244..6173)
                     /gene="cpaF"
                     /locus_tag="IXF88_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1508544.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6305..8059
                     /gene="ade"
                     /locus_tag="IXF88_001815"
     CDS             6305..8059
                     /gene="ade"
                     /locus_tag="IXF88_001815"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1508545.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8157..8819)
                     /gene="trmB"
                     /locus_tag="IXF88_001816"
     CDS             complement(8157..8819)
                     /gene="trmB"
                     /locus_tag="IXF88_001816"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1508546.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(8889..9668)
                     /locus_tag="IXF88_001817"
     CDS             complement(8889..9668)
                     /locus_tag="IXF88_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1508547.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(9875..11086)
                     /locus_tag="IXF88_001818"
     CDS             complement(9875..11086)
                     /locus_tag="IXF88_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508548.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11079..11816)
                     /locus_tag="IXF88_001819"
     CDS             complement(11079..11816)
                     /locus_tag="IXF88_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508549.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            11954..12388
                     /locus_tag="IXF88_001820"
     CDS             11954..12388
                     /locus_tag="IXF88_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1508550.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12381..12725
                     /locus_tag="IXF88_001821"
     CDS             12381..12725
                     /locus_tag="IXF88_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1508551.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
BASE COUNT         3960 a         2685 c         2155 g         4031 t
ORIGIN      
        1 ttaccatttt gcggtcttct ccgcaaaaaa aacacatgca agaattccct cttgcatgcg
       61 tttcatcttt tatcctaacg aatgctttgg cttagccgcg cagacgctcc acatcacggg
      121 caatcattag ttcctcatct gttgggatca ctaatactct tacttttgat tcttttgtag
      181 agatatctac ttcttcacca cgcaattcat tcaacgctgg atcgatttca caaccaaacc
      241 aagacatacc tttgatgatt tcactacgta catgtccgtc attctcgccg ataccagcag
      301 taaagacaat tgcatcaaca ccattcatcg ttgtcacata accgccgatg tattttcgga
      361 tgcggtccac gaaaatgtca taagctaaac gaatatgttc tttttcatag ttcttttcaa
      421 gatcacgcat atcactagac aatcctgtta ggcctagcag accagatttc ttgttcaaaa
      481 cattgatcat ttcatcaata tcaatctcta atttttccat taaatatggg agaacagctg
      541 gatcgatatc acctgaacgt gtacccattg tcaccccagc taatggtgtg aatcccatag
      601 aagtatccat ggatttacct ccatcgactg ctgtgatcga tacaccattt cctaagtggc
      661 aagtgatgat ttttagttct tcgatcggtt tgttcaataa ttttgccgca cgttccgaca
      721 cataacgatg acttgtgcca tggaagccat attttcttac tttgaaatct tcataataag
      781 acaccggtaa gctatataaa taattttgct ttggcattgt tgagtggaaa gatgtatcga
      841 atacagccac actcaaaatg tcaggtaaaa tctttttaaa cgctttgatc cccattaagt
      901 ttgcaggatt atgcaaagga gcaaattctg ctaattcagc aatgcggtcc attactttct
      961 catcgatcac tactgaatcc ccataagttt ctcctccgtg aacaacacgg tgccctactc
     1021 cggtgatttc atcataagag cttaaaattt gcagttcaat caactgatct aacagcattt
     1081 ttactgctac ttcgtggttt tcgatatcca tgatttgttc gaatttttga ttatcgccgt
     1141 acttgatggt aaaaatagaa tcttgtaaac cgatacgttc gacgatccct ttcgcaacta
     1201 cggtttcttc tggcatttga tataattgcc atttcaaact tgaactccca gcattgattg
     1261 caattgtttt agacatatga ctctccttct cgttgaatga ttacagagca gatgatttcc
     1321 agtttttaaa ctcgttgaaa aattccgtca ccttctctgg ttcttttaat gaagcaagtt
     1381 tcacaagcaa gacttctttt acttgttgtg cttgtggtcc ttttttctgt aagatcaaaa
     1441 tacttttttg cgattgttta ttacggaaca attcatctgg caattgaatc attccttgaa
     1501 gataaccttc ttctttaaac cattttttga tttcttcact ttgatccgtt tctaaaaatc
     1561 cactcggcat caaaaacaaa ccaaaaccat ccggcttcac atatttcatt gattgctcta
     1621 gcaataaatg atgtgcataa ctatggcctt cttcggtact tgttaaaaac tcttttgctt
     1681 tttcgtcatt cggataataa ccgattggca aatcgctgat tgcaaaatcg acaggatcta
     1741 ttaataattc ctgcaacccg tcttgatgaa agtattgaac atttgcttgt gtcaaatcac
     1801 ttgtagaagc tgcgacagcc aacaatgtat catcaatatc cacaccaatt ccttctgttt
     1861 ggtaccccgc attcgatagg tttaaaagaa ctgttaataa caagttgccc attcctgccg
     1921 caatatccag aatcttaacc ggtgcttttt tatttgtgta tagttgttca ataagaaaaa
     1981 caaataaaaa accgatacta tcaggagtga gttgatggtt cgcctgcaaa tgttctgtct
     2041 ggctgccctt caataaaagt aattgagaca gtctgcgcca ttcttctggc tcgaaagaaa
     2101 gtttcttcaa ctcttcataa agagctgtga tccgttgcgt tgtctctttt gtcggcactc
     2161 catccaccac acgtacttga taatcatcta ttaaattttc tgcattttcg acataggctt
     2221 ctaaaaagga agttcccaat gcattctgta aaagctgaat agcttccagg ttttgattaa
     2281 acccttgttc cattttttct ggtaacatgg tgcacctcat ttctagatat attataacaa
     2341 ttttttcacc tgtttaattc tatcgaacaa ttgacaaaat ttcaatcgtt ttcactgttt
     2401 tttatttgtg aaacggcaaa cattttttta ttaaaaacag tgttataaca gaaaagaggg
     2461 cgttttcacg aacattccat cattctgtac tagtatctgt atcatttttt acttcttctg
     2521 tgaaccgaaa gataaacggt gagacttcca cctctaagaa tagggactgt tgtacccact
     2581 gatatgttac agatccagtt gtgtagtaaa aaactccttc cgaactttcg gcaagcgtag
     2641 actgttccga taaagccatc tgcttcatta tttttgctat ataataattt ttgattcgta
     2701 tattaagatc cattgacaaa cggtagcttt ctaatgctgc catcaataaa aacgtacata
     2761 aaagaaataa aagcaagaca gaaaataaaa tagagccctt ataacatttt ctactgatcg
     2821 ttttttgtcc atatggcata tccatcttct ccattttcaa agtgtacata aaaagaaatt
     2881 gtgtgttctt tttccaataa ttgaagctca gtgatacttg ttaacatagg ttgatgtccg
     2941 ccggaagttc ttcttttacg tatcattgcc tgatatcttt cgatgatata ctgtttatcc
     3001 tcgatcgtgt agtgcagttg ctgatttttc acagagacat tgtgactgct cttgagttct
     3061 tcttctaatt gtgctaaaaa tattagccac tctttttgat ctttacgctg caaaaattca
     3121 cttacttgga tggtttgctg aatcaaaaga ttgaatgtga gcaatagact gctcaatatc
     3181 atcagtccaa ttagacattc aatcagcgta aatgctggaa ttacttgttt tttcaatact
     3241 gatcgcttct ttctccatat tgacaaacac tttttcgatt actccattat tatcgacaat
     3301 ttggatatcg tatgggatca tttcttccct tcgctctggt cttttgtgat acatccgctg
     3361 cgtcttgata tcttcataaa tcattcgaat aaatagcagt tcctccccac ttttcttttc
     3421 tgcctgaagc aaaaacagct ggccaaagct cactaagtag acacagacag aagcgatcat
     3481 aagcgctgta agggactcca acaaaacaaa tcctgtttta ttcaattctt tttgtatagc
     3541 gaccactccc tatctggaat tgatacgtaa tcgtccgctc tttttcttcc caataaaatt
     3601 gataagcttt tagtgaactt tcattcccgg ttctagcagc aaaagtaata gaagcatggc
     3661 gtttcaaggt aatctcatca ggaatatcta atacttccca atttatttta tctggattcg
     3721 gaacatgaaa aatgatttga ttctcttcac tattccaata aaagcctgtt tggatctgat
     3781 tcacaatagc gattttttgc gtagaataga ttcgtttttc aaattggccg aaaaattgat
     3841 agactgccaa ttcattcttc catgcagaaa atgcgagaac tggaaaagaa aggatcgttg
     3901 taagtaataa aatcagtaag agtgattcaa acaacgtata tcctgatgct aaaaccttac
     3961 cttcaaggcc gctgatagat ttctgcatgt tctcgtttga tatagccttc attcaacaat
     4021 tcctccacag taggaactct tccattattt tgaagcgcat agagctcttt ttgtgtgtct
     4081 accaactgga tgatcgcttc acgactttct tggtctacat gattcctata acgcgacaaa
     4141 ttagggacga acaacaacac taatacactg atgatcagta agacgattag catttcgatc
     4201 aatgtaaaac cgctataata agatattttt ctctttttca tggcaaaacc tcctctatgc
     4261 ttccgtaaac aggtaataaa acagcggcat aaactaataa aatcaaagcc gctacgccca
     4321 aaaaaatgat tggctgaata aaatgcatcc agtgatgaag acgtttgaaa agctgttttc
     4381 ttgtccattc gctgtaaaaa atcagttctt tacctaaact tccctttgcc tctccctgta
     4441 aaacaatttt actgaattct tctgatagaa aacgataatt ctggaactgc tcagccaatg
     4501 acatacctgc ctccaatcct tgaatcaact tagaggcaag atgctgaatc agactttctt
     4561 gtttcgtttc ctttaagcag actatgattt gccttaattc gaaaccttca tgaaaaagct
     4621 ttccaatctc tagtgacaaa taagccgatt gatacaagga atatatcggt ccaatcaata
     4681 attgtctgct tatccattca ctctgatcaa caggagacaa cttcatcaga cgatacctta
     4741 ctagtaaagc caatagagta accgctagga tgaatagtag aacataccaa tgaaagactt
     4801 gtatcagctg aataccccaa tgctcttgca tgaccattcc tgaaccaata agctgaggca
     4861 aaacgaacaa ccgcatacta aacaagatac ctagcaaaaa ggtgagcaat agcatcggat
     4921 atgcaagcag ctttttcaaa tcattgcgaa atccttcaac aagccgaaac tgtttggcaa
     4981 tttccaacaa agttgtcggc agattcccat gacgttcagc taactcgatc tgtaccagtt
     5041 gctctgctcc atatcctatt tgcgaaagta cctcactaaa cgcttgccct tcctttagct
     5101 gatgttggat atttgccaaa ataacaggtg gaaacgaacg gatcgtgagt aataaataaa
     5161 gggattcttg cagactaaaa ccagataaaa gcagttctcc catggtttgt gcaaactggt
     5221 tttgctggct tcttgttaat cctttagaat agttctgact gccaaatatt ttcctgagct
     5281 tcttcataac tttcctccca tgattttttt actgattctt cctccataaa ttgataactc
     5341 cataaaacag aaggatggtg attggcatct tccaaaagtt cttgataaat gacaccagct
     5401 aaacattcag atagctcttc ttttttcttt gtcagttcat ggattctgac caatgtacct
     5461 tcaagattgc gtgcatgtac ggttgcaaaa acccggtgcc ctgttagtgc tgctcttacc
     5521 gcagcatgtg cagttgattc atctcttatc tcgccaatga ttagcaagtc tggacgatgc
     5581 cgtaatgcga gtctcaaaag tgtatcataa gtctgtgcta ttttcggatt gatctgtagc
     5641 tgcagaaaaa acggttcttc tatctctaca ggatcttcga tagtaatcac ttgcaatttt
     5701 tcttccaaag ccaaccgata cattagagaa gtcttgcctg atccgactgg tccgctgaaa
     5761 aggtataatc cacgtctgac ggttttctgt aaaatagttg caaaatctcg agaactaaag
     5821 caacgatggg gtgtttcaaa agtaccgtat aatagccgaa tcactaaact ctctcgttga
     5881 aggtaatctc ctacagaaga caaccgcaga cgaacttcct gtttttctaa agaataagta
     5941 aatgcaccta actgagcttt tcttttttct ccgatatcca tctgtcccaa atacttaaat
     6001 cttgagatca gttgctgtcc atctgccgaa gagactttct tccagaatgt tcgttttttt
     6061 ccatagcgaa aatagatgta aaatacttct gaagctggca atatatatac gtcttgggca
     6121 tgtttggcaa aactttcact tatcatttga tccgataatc gttttatttc cattattctc
     6181 ccctccacaa aaaacatacg tctttttgat ctttttgttt caaataaaat gaatttacaa
     6241 aaaaatattg gcaaaaaccg aatttatgtt atacttataa aaatttatct tgggaggcac
     6301 tccattggaa acaacattaa ggaaatatat cgaagtagca agtaaaaaaa agcctgctga
     6361 cctagtgatc aagcatgcta agatcgtcaa cgtctttacg aaagaaatca tggaaaaaga
     6421 tgtagcaatc tgcgaaggaa tgatcgtagg aatcggtgaa tacgaaggaa aaagcgtaca
     6481 taatgctaat gggcaatatc tagtgccagg attcatcgat ggccatgtgc atatcgaaag
     6541 ctcactttta tcaccaagcg aattttcaaa agtatccttg cttcatggag ttactactgt
     6601 agtgacagat ccccatgaaa taggaaacat tgctgggagt acaggtcttg agtttatgat
     6661 agaagatgca agacaaacac tcatgaatat cttttttatg ctaccttctt gtgtacctgt
     6721 aacccctttt gagaccaatg gtgcaaattt aaatgcaaaa aaattagccc cttttttaca
     6781 aaaacccgaa gttttaggct tagctgaagt catgaactat caagcagtag caaataatga
     6841 aacagacatc ctagaaaaga tccaattgat gcaccagcat aagaaaaaaa ttgacgggca
     6901 tgcagctggt atcggcatgg aagagttaaa tgtttatcct gcagcaggta ttcggacgga
     6961 tcatgaagct acaactgcaa aagaagctaa agaacgtttg gatcttggta tgtatttaat
     7021 ggttcgagaa gggaccgttg caaaagatct ggcatcgcta ctaccggcga tcacacctga
     7081 aaattcaaga agatgcttct ttgtcaccga tgataaactg atcaatgatt tagtaaaaga
     7141 aggatcaatc gatcatatcg tccgtcaagc tattgcactt ggaatcgatc cattacaagc
     7201 cattcagatg gcaacattga atgcagcaga atgttttgat ttgaaaacac taggtgcgat
     7261 tgcccctggg tatcaagctg atttcttctt aacagacgat cttaccaagc tgccaattca
     7321 tgaagttttc tataagggaa cattagttgt tcaatctggg caactccaag accatttatt
     7381 tgctgatcat tcaaattcat atacgtgttg tctgccaaaa ctaaacgctc aaccattaaa
     7441 tccacattcg ttagagctgc ttctttcttc ttcacaagcc catgtgatcg aaattatccc
     7501 gaatagtcta gtgactaaag atttggtaga aaaagtggac cgacaaggcg aacgattcat
     7561 tccttcaatc gaaaaagacc aattaaagat tgcggtgatc gagcgtcacc atcaaacggg
     7621 aaacattgga ttaggcatag tgaaaggatt tcagctgaaa caaggagcga tagcgacaac
     7681 gattgcccat gactcgcata atctagttgt cgttgggaca aacgacgagg atatgctgta
     7741 tgctgctaat cttttgattc aaaaaggtgg cgggatgacc gttgtcaaca aacagcagga
     7801 aatagcttgt gtttctcttc ctataggcgg aatcatgaca caggaacctt tttcgatcgt
     7861 ctatgaacag ttgaatgaat taataactaa agcacatcaa ctaggagcag ctaaaacctt
     7921 tgatcctttt ttgacacttt ctttcttaac attatctgtc atacccgaat tgaagatcac
     7981 cgacaaagga ttattttcat tttctcgctt ccaattgatt ccaccttgtg gaaaaaccga
     8041 tgaaataaat acattttaaa tcaaataaat cccagaaaat acattacatc tgtaatgttc
     8101 attttctggg atttatttat ggatattaat gtttttatga aagcagaaag ttagtttcac
     8161 agacttttct ttttggtctt atcttgaaat tgagcttcta ctcgataaat acgttgacct
     8221 tttgaagaaa atttttcttc atactccgtc ataatattgc cttcaaattg actttgatgt
     8281 aaatctaacc atacctgttt gaggatcatg ccatattgag aaaaacttgc cagagaatac
     8341 tcaaacaatc cttgattatc tgttttgaaa tggatttctc catttggacg aagaatctgt
     8401 tcatctacag caagaaaagt cttatatgtc aatcgccgtt tttcatgacg tttttttggc
     8461 catggatcgg aaaaattcag atagatttga tctacttcgc tgtcagcgaa atactctgtc
     8521 aacgctgatc catccacatg tagaagtttc aggttaggaa gcggttgttc gatcaacttg
     8581 tctagtgcaa tcgaaacaac actgacctgc atttctactc caatataatt gatttcagga
     8641 tgggcttttg ccattccagt gatgaactgg cctttcccca taccaatctc aatgtggatt
     8701 ggatgatcgt tttcaaacaa ttcattccat tttcccttcc acaatgttgg atcactaatt
     8761 acaaaattcg ggtgagccgc taacatttcc gctgctcccg gacgatttcg gacacgcatt
     8821 atttatctcc attcctttcc tctaattaaa aaggtctggc caaatctccg cagagtttgg
     8881 gccaaacctc agccactgaa aattcgtttt aattgtaaaa tttcttcgtt catccgtcga
     8941 tgatcttcac gtttgcaata tctggtgatt tcttggagca aattcatggc cgcataccaa
     9001 tgaaattttt caagcatttc gtcagtcggg cgtatgccat aactgatcag ccactgattc
     9061 cagctagaca gtggcacata atttcctaaa atcgttgcaa tatccaccgc agggtctgca
     9121 aacatgattg aatcccaatc gactagatat aaataatttt tacatactaa ccaattacga
     9181 tggtttacat ctccgtgtac tactgataag ttttccttat ctattgacgg gatctgctca
     9241 cgaagaaaac gatggacaat ttgcagaaat tgatttttct ggagttcttc cggtaattct
     9301 ttttcatatt tttgcagcat cttctccggc gtcatgacac gaccaccgat acgggtcagc
     9361 atggttttaa gagaatttga atgatgtaaa tggtacaaaa catcaatcac gtcgtttctt
     9421 tgaccaatct cattaggagt cagcaagcga ccttccagcc actcttgtgc tgtcaaagta
     9481 tcgccattcc ccgttcgttt tgtccaaaca agttttggtg taattccttc ccttgaaaga
     9541 gcagccaaca tcggagtcgt gtttcgttta ataaagactt tgtcctcatc acgcatgcct
     9601 atgtatgttt ttcctgtgtc gcctttgatg ggacgtaacc gccactcttg gtctaactga
     9661 aactccatca tgcattctcc cgtcctctta ctctttgttt tgcagtactt attattctag
     9721 tcgcctgaac actacacgtc aagcacatat acgttctttt tcatttaatt gccattcaaa
     9781 aaaccagctt ttctcaaaaa aaatagaacg aacagcattc ttactgtcaa cgctctattt
     9841 ttcattctca tttttaccct acacagcttt gttttcagtc tgacatcttc ttcaaacgag
     9901 ttggtacata tagtttaaca aagagcatag taacaatcaa ataagccgct agcggaaccc
     9961 atcgctgctg cccatctaat acgactgcag caatcaaacc aaaaataacc gacgcgataa
    10021 ttaataccca tgtgagtaga tactgaatcg cctgcttctt ctgattctta tggattggat
    10081 aaagctgaac gagaaccata tagcggaact ggttcgctaa aggtaacagt tggaaaccga
    10141 tcaaatataa aaataagaaa ccgataatca gactaaaata aaagtcgact actccggcaa
    10201 tcaataaccc acctataatt actaatcgga aataaagacc actaaaatca gaacctcttg
    10261 ccaatcttct tgcaaacaag taaagataag tgttttttga ttctctttta atatgagtca
    10321 atataggatc caaatagcgt cttcttttga tctttgccgt aatttcagga acatctgtga
    10381 ataaattgat aaattgatag atctgatgca acctatgttc ttctacatgc accattttct
    10441 cccaatccaa tcctttttct tgttgcttcc acaaaaacca gtaatataga cttgcctgta
    10501 ctaatgcacc tgctattcct gcccatatta gccaataaaa tgatagaaac aaaaagatca
    10561 gagaagaaaa aaaccagcct aatttccaat aacgaataac ttgttgatct agtcgaaaca
    10621 attctgctct cttgatttgc agatgactga atttcaaact ccataatatg atcagataga
    10681 cgaaaaacat cgaaaaggat tgctccgtac taaccaccac taatggcatg gccaatccgc
    10741 atgccaatat caaaaagatc aacggaaaaa tgcaagaata gcgaaaagct cgttccaaat
    10801 aggtttgcat ttctttttct ttaggaagta aaaaaacaat atctgctgcc tctgcaaaag
    10861 tagccatctt acccacgaat aaacagagca tccaaaatac agagattatg agccctccaa
    10921 ttgcaaacgg tggctgcagg gttttcaacc aattagaata atataatccc acaccgccaa
    10981 tcaagaaaag acaaactaaa acaaaatgat cgttcaatac ataacgcata tatttcatca
    11041 ttttcttttg atgtcttgct aaacgttttt gaaaaaaact actcatgtgc ctgctccttt
    11101 gttaaagcaa tatagatttc gtccaaagaa gaatccggca gctgaaattc ttttctcaac
    11161 atctcaagag aaccgtctgc tcgtatctct ccgttatgta agaccacaaa acgatcacaa
    11221 tatttttctg cagtagctaa aatatgcgta gacatcaaaa taccggctcc ttcttttttc
    11281 atttctacca tcaattcaag caatgcattg atagctagtg gatccaagcc taaaaatggt
    11341 tcgtcgatga tatataaaga aggctcgatc aaaaaggcgc ataagatcat cactttctgt
    11401 ttcattccct ttgaaaaatt agctgggaac cagtcaagac gattagataa ccgaaaagtc
    11461 tccaacaact tttctgctct tttcattgct atatccatag gtatatcgta ggccatcgca
    11521 gtgacttcga tatgttctct caatgtcaat tcttcatata aagaaggagt ctctggaata
    11581 tacccaattt gtttgcgata ttcttccgta tttttcttta aggatagatg attgatttct
    11641 atcgttcctt ttgaagcttc cagcagaccg ataatgtgct tgatggtcgt acttttccct
    11701 gctccgttca gtccaatcaa ccctactatc tctccaggct gtacatcgaa actgatatct
    11761 tttaacacag ggagatgtcc gtacccccct gtgacatgat cgataattaa actcattctt
    11821 tttcctccgc catttatttt tatttattat accacgaaga aaatgacggt attttgtttt
    11881 tttccttaca atagctttaa cttatggtaa agttaagctg aagtatttat tctttataga
    11941 aaggatgttt tatatggaaa actgtatttt ttgcaaaatc atcaatcaag aaatcccaag
    12001 ctataaaata tatgaagacg acaaggtcta tgctttttta gacatctctc aagcaacgaa
    12061 aggacacaca ttagttgtgc ctaaacgtca tgtagcagat atttttgaat atgatcccga
    12121 acttgcagga gaggtttttt cgcgtgtgcc taagattgct caagcattgg aaaaagcttt
    12181 tccagaaatg aaaggtctta atatcttgaa taacaaccgt gaactagcct atcaatctgt
    12241 ttttcattca catatccatt tagtccctcg ctacagcaaa gaggatgatt tttctattca
    12301 ctttgtcaat catcaagata gttatggatc ggaagaacta aaagctattc aagaaacaat
    12361 cgtaaagcag gtgtcctgcg atgattaaaa actttttcaa aggactgtta tttggagctg
    12421 ctgctggcac tgtcggaggt ctgttattgg cgcccaggag cgggaatgaa acaagaaaga
    12481 aactgatcga agaattggaa gaagcaacaa ctctgacaaa tgatttaaat aacagcctga
    12541 atcattttaa aagcgcattg atcgaaacaa aagaaacagc tgaatctgtg attcctgctt
    12601 tccaagaagc aatcaaaaaa gatatcgaag attttaaatt ccaagccgaa ccacgtatca
    12661 cacaaatcaa tgaacaagta gaaaaactaa aagctgatct gccagagtat cctgaacaaa
    12721 tataataatg attattttct caacagattt aactatataa gtgagaaaag tttgcgtatt
    12781 tcctaggaat gtgatgaaat tttgaatttt gtttgaaact accacgacaa c
//
LOCUS       DACESX010000078        12823 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.078, whole genome shotgun sequence.
ACCESSION   DACESX010000078 DACESX010000000
VERSION     DACESX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.078"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..137
                     /locus_tag="IXF88_001822"
     CDS             <1..137
                     /locus_tag="IXF88_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1508552.1"
                     /translation="VGSIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL
                     "
     gene            210..326
                     /locus_tag="IXF88_001823"
     CDS             210..326
                     /locus_tag="IXF88_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ1508553.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(381..2063)
                     /gene="recN"
                     /locus_tag="IXF88_001824"
     CDS             complement(381..2063)
                     /gene="recN"
                     /locus_tag="IXF88_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1508554.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2081..2530)
                     /locus_tag="IXF88_001825"
     CDS             complement(2081..2530)
                     /locus_tag="IXF88_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1508555.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(2637..3455)
                     /locus_tag="IXF88_001826"
     CDS             complement(2637..3455)
                     /locus_tag="IXF88_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1508556.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(3464..4354)
                     /locus_tag="IXF88_001827"
     CDS             complement(3464..4354)
                     /locus_tag="IXF88_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1508557.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(4356..4580)
                     /locus_tag="IXF88_001828"
     CDS             complement(4356..4580)
                     /locus_tag="IXF88_001828"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1508558.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(4585..5922)
                     /locus_tag="IXF88_001829"
     CDS             complement(4585..5922)
                     /locus_tag="IXF88_001829"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1508559.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(5923..6777)
                     /locus_tag="IXF88_001830"
     CDS             complement(5923..6777)
                     /locus_tag="IXF88_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1508560.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(6874..7323)
                     /gene="nusB"
                     /locus_tag="IXF88_001831"
     CDS             complement(6874..7323)
                     /gene="nusB"
                     /locus_tag="IXF88_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1508561.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(7316..7741)
                     /locus_tag="IXF88_001832"
     CDS             complement(7316..7741)
                     /locus_tag="IXF88_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1508562.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(7987..9051)
                     /locus_tag="IXF88_001833"
     CDS             complement(7987..9051)
                     /locus_tag="IXF88_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1508563.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(9223..9468)
                     /locus_tag="IXF88_001834"
     CDS             complement(9223..9468)
                     /locus_tag="IXF88_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1508564.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(9607..9981)
                     /locus_tag="IXF88_001835"
     CDS             complement(9607..9981)
                     /locus_tag="IXF88_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1508565.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10064..10978)
                     /locus_tag="IXF88_001836"
     CDS             complement(10064..10978)
                     /locus_tag="IXF88_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1508566.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(10999..11802)
                     /locus_tag="IXF88_001837"
     CDS             complement(10999..11802)
                     /locus_tag="IXF88_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1508567.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(11835..>12823)
                     /gene="manX"
                     /locus_tag="IXF88_001838"
     CDS             complement(11835..>12823)
                     /gene="manX"
                     /locus_tag="IXF88_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010052499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1508568.1"
                     /translation="VGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDLR
                     AKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAYA
                     SRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGDG
                     KIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVKA
                     NVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKVV
                     VSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
BASE COUNT         3579 a         2900 c         2140 g         4204 t
ORIGIN      
        1 tcgtcggcag tatctatgga atgaacatta agttacctat tgctgaaagg gatgatgcct
       61 ttatatggct agttctatta acattattta tttgtatcgt aactacctat tatttaaaaa
      121 aagggaagtt tttataaaaa agccctcttg tggaaatgga aaaaagattg tacacttgaa
      181 tcaactaatt aatgacgagg ttgaaaacaa tgcgtgaaaa aaaacaaaca agtacttttg
      241 ctaaagtaac aaaatttgtc atttggacaa tgctgatttt aacgattgga tctgttgtat
      301 tgactgctgt aatgagtgtc atgtaaaaat aaaatatagc tatcagctgc tgaaaaggac
      361 ctgaaccact gcgaggtggc tcaggtcctt tttgcgagtt tcaatagttc ttttgcatgt
      421 tcgagtgtga gttttgtaat ctcacttcca gccagcatac gtgcaatttc ttcttctctt
      481 tcttttgttt tcaactctga aacagaagtt tgcgtacgtc catcaatcac tgctttcaca
      541 atataatatt gatagtcggc gactgcagca acttgaggca gatgagtgat gcacaaaact
      601 tgagagtatt tcgatatttg agaaatttta tctgcgatgg cctgtgcaac tcttccgctc
      661 acacctgtat ccacttcatc aaaaataata cttgtcacac cttgctcaga agaaaaaata
      721 gttttcaaag caagcaacat ccgagacagt tcgccaccag aagcgacttt caccaaaggc
      781 ttcagtggct ctcctggatt ggtcgtaatg taaaattcga tttggtcaaa tccatcatga
      841 ttcaattgtc tattttcttc ttttaagaaa cgtacctcaa actcagtgtt ttccatatac
      901 agacttttca attcatgcag aatggatttt tccaattctt ttgcgatttt tttacgcgct
      961 tcatgaagat ctgcggcttg ttgctgaagt agaagctctt tttgctctag ttcctctttc
     1021 attttgtcca attgaccttc tgaaaaatca gaagaagcta gctcttcggt gatttcgtca
     1081 tagtaagcca atattgactc aatggattcg ccgtatttac gtttcaactg tcggattaat
     1141 tctaaacgtt gcgtaacttc ttctaaacga ctctcatcta actccaataa atcaatctga
     1201 cgactcatat cgccgattgc atcttgcagc aaatagtaag cactttggat attttcataa
     1261 attgcttcat actccacatc tagatgggcg atcccttgca gttctgaaac ggccaagcca
     1321 acaccatcta cactgttttg ttcattattc gtcagtgctc cgtaacctgc agctaatcca
     1381 tcagcaattt tttgataatt gctaagtttt tctctttctt cttttaattt ctcttcttct
     1441 ccgacttgca aatcagcagc agctatttct tcttgctgga aatggagcat atctattctc
     1501 tgaacatagg atttttcatt ttgctgcact tttcgtaccc ttttttccag ctcacgataa
     1561 gagatatatt cttgctgata actctttttc ttctgttgga aagctacatc tccaaaacga
     1621 tccaataaag caagatgtga ttcaggctgg agcagttcct gatgttcgtt ttgtccttgg
     1681 atatctacta aataactgcc gattcttctc agattagcta atgtgataat atgaccattc
     1741 actcgacaaa cattttttcc agttaaggac atatcccgtc gaacaatcag attgtcctca
     1801 tctgtttcga tccctaattc aaccatcagt tcagagaatc cttcttgttt tggtagttca
     1861 aacaaccctt caagcacaca tttttctgct ccttgacgta tataatcgct tgaacctctt
     1921 cctcctgcta acagacctaa agcatcgata ataatggatt tacccgcacc agtctctccg
     1981 gtcagcgcgg tcattccttc gtgaaaagaa agccgcaatt ccggaataat cgcaaagtta
     2041 gtaatactga tttcttgtag catcacattc gctcctcaac ttaaaggtaa tgcaggaagt
     2101 ctttcttcaa taaaactgcc tggtcttcag ttcgtgtaat catcaaaaca ttatcgtcat
     2161 cattgataat cgaaaataat tctttcttgt aacgtttatc aatcaaattt gctgctgccg
     2221 aagcatttcc cggcaatgtt ttcaaaataa caaatttttc catttggtca actgccacga
     2281 aagcgtcttt taatagtttt tccaatttag tatttacatc ttcgtttgtt tcagctggta
     2341 tgctataacg gtagccgcca tctgaagccg gtaccttgat cagtttcagt tcctttatat
     2401 ctctggaaat cgtagcttga gtaacagata ttcctttgct tttcaggatt tcaacaaatt
     2461 cttcctgctt tcggatatct tgctcactca acaatcgagt aatcaaacga tgccgatctt
     2521 tttttctcat tacgtcttcg ctccttagca tattcttctt aatcaatgat agcttatttc
     2581 cttggtataa taaagaagaa tccgcatact tctcacttga ttttcacagt tacaatttat
     2641 ttatggtcaa actgttcgtg tgcatttgaa accacttctg ccatattaat agctgaattg
     2701 attgttccgc tttcgggtac tttcctcaag tgagccaaga actcgatatt tccttctccc
     2761 cccgtgatcg gagaataact taattccagc acatcatatc caccttgtgc cgcaaatgac
     2821 aaaatgtctt ctaaaacttt ttgatgggtc tgagggtcac gaacaattcc gtttttccct
     2881 acagcttctc tgcctgcttc gaattggggt ttgattaaag caatcacttc tccctcttct
     2941 tttaaaatag catgtaatgg aggcagcatc agttttagtg aaataaaaga aacatcgata
     3001 acagcaactt ctggaattcc taaggtaaag tcttcgggtt ttgcataacg aaaattcact
     3061 ctttccatta cgacgactct aggatcttgt cttattttcc aagctagctg attgtagcca
     3121 acgtctaaag cataactcat ttttgcgcca ttttgcaaag ctgcatcagt aaatccgcca
     3181 gtggatgcac caatatctaa caatgttttt ccttcaacag atagatcaaa tgctttgagt
     3241 gctttttcta attttaatcc ccctcgagaa acataaggaa gttttttacc tttgactctt
     3301 aattcactac tcactggaat tttttctccc ggtttatcaa accgctcgtt tttttcgttg
     3361 taaatcaaac cagccatcac tgaacgtttg gcttgttctc ttgtttcaaa taatccttgt
     3421 ttgaccgcta atacatctac gcgttctttt tccattcttt ccctcatttc tccacatcaa
     3481 attgcttgat catctggcgc atcagcccgc catcaaaaga aaaaacgtcc tcttctaatt
     3541 tgtctatgat cttgttggcg gataacagct caatctccaa cgcctcacgt gtcttttcta
     3601 ggcctaataa acgcgggtaa gtattttttt ccatacgttc atctttacca gctgttttcc
     3661 ctaatgtttt cgttgtacca atcacatcga gtagatcatc tcttatctga aaagctaaac
     3721 ctaaatgttc ggctaatcgt tgaagtagca ataagatttc ttcaggttgc ttcgctaata
     3781 tgccaccagc cagcaaggca taacgaatca aatgccctgt ctttctttca tgaataaatg
     3841 ctaattcttc taaagaaagc tttttcgatt cgccctcgat atctgcagct tgcccagcaa
     3901 ccataccttg tgatcccgca caaacagcta attgctgaag cagcagaagc ttaggagaat
     3961 tacctaagtg tgccatactg attagctgga atgctcctgt aagcaaacca tctccggcca
     4021 atatagctaa tgcttcgccg aatactttat gattggtagg ttttcctcta cgaagatcat
     4081 cattgtccat cgcaggcaaa tcatcgtgga tcaaggaata cgtatgcacc atttctaaag
     4141 cagctgctac ttgatagacg ggaacatcaa taggctcatc aaaggaagca acggccgtta
     4201 aaagtaataa gggacgtatt cttttcccgc ctgctttcac tgaataactc atcgcatcat
     4261 agagtcctct atccgttgta tgctcatcta aaaaagacaa gatttctttt tctacagcag
     4321 gtagatgaat agctgaaaag tcagttaatc tcatctcagc tttcctcgtt tccgtcaaaa
     4381 tcaacttctt catttgattc tgtcatcatt ttcgtcaagg tctcttctgc tttagctaat
     4441 gtatcttggc aatgtttgct taattccatt cctcgtttaa acgaatctag agctgattct
     4501 aatggaacgt ctccttgttc taactgcatg acaatttttt ctaattcttg cagtgactct
     4561 tcaaaagttg gattttccat atttttattc tcctttgtct atctcttgta cttgtccttt
     4621 aacgattcca tcttgataat ggatcgtcag caaatcccct tgatgaatct cggtgacact
     4681 ttttactact tgatcattta acgtcgtata gctgtagcct cttcccatga ttttcaatgg
     4741 actcaacaaa tcaagagatt gaacagcttg agcaaaacgc tgctgctgtt gtttcatata
     4801 ttgttggatt ttctcttcta agcgtttttc tagataagca gtttcttggc gagctgcttt
     4861 aactcgataa agcggagcca cttgttccaa ttggtggctt aatgcgatcg tttgtttttc
     4921 tttcgcatga taaatttgct gtgtagcttg atataatcgc tgtttcaact ggtctaattt
     4981 gatggtctga ccttcataca atctttcagg ctgccgaaat acataggaac gtcgagcacg
     5041 ctcgaaccgt tcttgtttgc gctggatctg gcgtacaaac gcctgctgca atctagcttg
     5101 ccgttcttga atgcgcatca actcttctga caaaacagga acagctaatt ctgcagctgc
     5161 tgttggagtg gcagcccgaa catctgctac taaatcagcg atcgtggtat ctgtttcatg
     5221 tcctacagat gaaatcaccg gcgtattagc ttgataaatc gcccgagcaa cccgctcttc
     5281 attaaacggc cataaatcct caatcgaacc acccccacga ccgatgatca ttgtatcgaa
     5341 attgcccatc tcttctactc tctggatatt cctgacaaca ttgtccgctg cttggtttcc
     5401 ttgtacaact gttgggaaca aaaccaattg gacaattgga tatcttcttt tcaccgtcgt
     5461 gatgatatcg cggataaccg ctccgcttgg actggtaagg accgcgattc gctttgggaa
     5521 acgtggcagc atttttttag gcccttgaaa aaggccttcc ttcatcagct tttcttttcg
     5581 ttcttccaat tcttgatata aagcacctat tccatcaggc tccatgtgct ctacatagat
     5641 ttgataagaa cctcccgctt catataatga aatacgccca acgacaagga ctttcattcc
     5701 ttcttttggc tggaatcgta atttttggaa tgctcctttg aacataatag cagatatttt
     5761 tgcatggtca tcttttaagc tgaaatattg atgggcattg gcacgtaaac ggaaattcga
     5821 tatttcgcct gttaaatata cgcgttctaa ataaggatcg gcatcaaatt ttcttttcaa
     5881 atacttcgtt aatgtagtga cagttaaata ttcttgtgcc atctattcac ctgctccaaa
     5941 agcttcggca gattttacgg tttgatacat cagcatcgtg atagtcattg gccctacacc
     6001 ctttggcaca ggagtgatcc agcctgcaat aggctccact tcatcaaatt tcacatcacc
     6061 gatcagctta ccgttagcat cacgattcat ccctacatct atcactacag cacctggttt
     6121 tacgaattct tttgtgacaa aatgtccacg tcctattgct acgactaaaa tatctgcttc
     6181 ttgtgccagt tcagccagat tttcggtttt agaatgagca acagtaacag ttgcatctgc
     6241 catcaagagt agctgagcca tcggtttacc gacgatattg cttcgtccga taactactgc
     6301 tcgtttaccc gccggatcga tagaataagc ttcaaacatt ttcatgatgc cgtatggagt
     6361 acaaggaatc ttgtccggtt tacccgcaag gagtctgcct aaattcatcg gatgaaaacc
     6421 gtctacgtct ttttcagggc ggatagctaa caggatcttt tcttcatcta tatgctttgg
     6481 caatggtaat tggaccaata taccatgaaa actaggatct tgattgtatt tttcaatttc
     6541 tgcaagtagt tcgtcttcag aaatcgtctc aggataacgc tctaccaatg aatggattcc
     6601 gatttctttt gctacacgtt ctttatttct tacatagatt tggctggctg gattttcccc
     6661 taccaataaa acgactaatc cgggatggat accattatct ttcaattttt ggattttttc
     6721 agcaatctct gcctgcattt tttcagctaa ttcttttccg ttaatcaatt ctgccatcta
     6781 accactccca gatatttttt gtgttttcat tctaccatag aattttcccg atttataaca
     6841 ggtttatgat gattcataga aaaaagctag gacttacgcc ccagcttcta attctttcaa
     6901 tacattggat aaaacaccgt tgacgaattt acgtgaacgg tcatcactaa atgtttttgc
     6961 caactcgatt gcttcattta aagcaacagc cgctggtaca tctgtcacat aagtcatttc
     7021 aaaaatcgcg atacgcaaga tcaccaaatc catttttgag atccgattga tgtgccaatt
     7081 gttttttaaa tgtttttcaa tcactttatc caattcatct ttttttgcac agacaccgcc
     7141 aactagcgta tcaagataaa caggaacaaa ctctgattca tcttcgttga tcatgtcgcg
     7201 gtggtctaat tcgattgcgt tgaaaatcgc atcttctttt gttaggtctg cattgaagtc
     7261 taatggaaat aaagcctgca atgccatttc ccgaatctcg tgtcttgata attctttatt
     7321 cattctcttc ctcttcgtct tcaaaaagtt cttcgattgt tggttgttct aatttttcag
     7381 gaacaacagc tactacatgg atattgactt cagataaatc gatatccgtc atgaaaagga
     7441 cttgctggct gacccgttcc tgcatttcca ttgctacttt aggaactgaa acaccatatt
     7501 ctagataaca gtaaagatct actttcaagc catcttcctc gtttgctaag taaacacctt
     7561 ttccgtgagc tgcacgtcct aataattcag tgacattgct ggcaaaagtt ccgcgcattc
     7621 cgtatacgcc ttctacttta gaagctgcaa tcccgataat tacttcgatg acttcaggag
     7681 cgatcacgat ctctcctaaa tcttggtttg cgtttaaaat caaatttttt tcatctgcca
     7741 tttatttatt cctccctttg cctaagcacg agatacatcc gaattctcta tgatgcggca
     7801 tagtgatcac gtggtaagaa aactgccgcc gatcagctgc aaactgctat gaattggaga
     7861 aaacacccaa ctttattatc tcttacagct taaaagttct tctatagtgt atcattaaat
     7921 cgagtgattt tctataacaa agtctgtgaa cactgatttt tacgcttact tcttctattg
     7981 atgaaactat aagatgatca attcttttgg tgaacgagtc aacacacgat ttccatcttc
     8041 tgtgatcaac aagtcatcct caatacgtac tccaccgatt cctggtaagt agatccctgg
     8101 ttcatcagtg atcacatttc ctggtacaaa ttgcttttct gcacgagaag aaacgtttgg
     8161 tccttcgtgg atctctaaac cgatcccatg tcctgtagaa tgtccaaatg cttccccgta
     8221 accatgtttt gaaatataat ctctagcaac tgcatctaat tggacaccag tcattcctgg
     8281 ttttgcagca tcaataactg ctaattgagc atctaagact atttgataaa tttcttttaa
     8341 tttttcccca ggatcaccaa tagcgaatgt acgtgtcata tcggaaacgt acccttcgta
     8401 atagcaacca aagtccaatg tgatcaagtc accttgttcg atgatttttt tgcttgctac
     8461 accatgcggc atagccgaac gcaatccgct agcaacgatc gtatcaaaag aaacacctga
     8521 agcgccaaga gaacgcatat aaaagtctag ttgatttgcg acttcaatct cagtcatgcc
     8581 cggttggatc attttcagga tgtgatcata tgccatatcc gcaatatgac atgctttttc
     8641 gataatagcg atttcttctt catctttcac ttctctgagt tcttcgatca tctgtgaaac
     8701 aggtactaat tccgcatcga tgatttcttc taaaaccgaa tattctaaaa atgagactgt
     8761 tgtttcctca aaagctaatg agtgcagttc ttctttgcgt actaagtttg ctacctcatc
     8821 aaaaataggt ccgacatttt taataatttc aaacccttga gcctgcgctg ccgcctgctc
     8881 ggtataacga aaatcagtaa taaaaaatgc tttttccaaa gtaataacag ctaatcctgt
     8941 cgtacctgta aagttcgtta agtaacgaag attgtacgga ctggtaacca aaaatgaatc
     9001 caaattttct tcttgcattt ttttgcgcaa tttttctaca cgtagcatca taatgaaact
     9061 ccctctttct ttgcacgctt ttttccatta tagcaaactt ttcttcgtac acgacagtta
     9121 aataaaaatt tccggaaaac ttctttttat tttaaataaa taaagccaaa aaggagtcac
     9181 agtctcgctg caactccttt tagtttacta ttttgtaaaa acttatgatc gttttccaaa
     9241 gaaaaatgag acaactgcta ccaaaatcac tgcgcctaaa atagatggaa taagtgccat
     9301 acctgcagct tttggtcccc attcgccgaa aatcgcttgt ccaatacttg aaccgactag
     9361 tccagcaatg atattcgtta cacagcccat cgaatttcct ttactggtga tcgcacctgc
     9421 aattgctccg atcacggctc caacaatcaa tgtccataac caactcataa tataacctcc
     9481 ttctttgcta atcaatttta gcatatggga tttaaagggg acaaataatt ggttttgcta
     9541 tttcatattt tcttctgcaa agtaaaaaaa taaagaaaac ctcctcttag aagttttctt
     9601 tattttttac ttgcgtttaa acatactacg aataccacgt ttcactacat caaagaaact
     9661 caatgattgt accattctat cttcatctac atattctctg atcgcacgaa gcactttttt
     9721 aggttctttg gaagaaaatg tgtatgtacc attttttttc gtttgaatgg catatcgagg
     9781 aatccatttt ccctttagca tgactgatgc tagtacataa tccacttctt cccatggaat
     9841 ttggatatat ttgcgtctat ctcgcgcatg gtaaaactca aaaccattat cacctatcat
     9901 gatttgcccg taatctgtca gacccgtaaa cgaagtagca tcaattacca agtctacttt
     9961 tgtattgatt gattgaacca ttttctccct ccagatattt gtttatgaac agtatactat
    10021 ataaaaaaag ctcgggacaa cgcccgagct atatttttat ggattataaa agtccgatga
    10081 cgtgtccaac aacaccgact acgaataaac caaggatgat aacgattgga gaaacctttt
    10141 tcttaagcaa ccacatacat aacaatgtta atcctaatgc agctaaacca ggaatcaatt
    10201 ggtctaagtt gtcttgtaat gttgttacct tttcaggaga aagggctaag ccattattaa
    10261 cttgttcaaa tgcactcttg attccttcac cacctgaagg caatttatcc cattcgatgt
    10321 aagcaccttt atctaatttt acagttgata caactggtaa gaatttgatt gatacccatc
    10381 tttctacaag ggcagctaat acgaacatcc ctaaaattga agcacctttt gttacgtctt
    10441 gtaacaatcc gccagaaagg tcatcagtaa ttttagaacc agctttgtag ccaaattctt
    10501 gtgtatacca catgaatgcc caacgaataa tgttccaacc taagaagaag atgattggtc
    10561 caaggatatt tcctcccata gctaatgaag cacctagtgc acctagcatt ggacgtactg
    10621 taaaccagaa cactgggtca ccaacaccag ctaaaggacc catcatacct actttaaccc
    10681 cttgaatcgc tacatcatca acaggcgcgc cattcgcacg ttcttcttcc agagccaatg
    10741 taacccctaa aattggagaa gcaatgtatg ggtgtgtatt aaagaattcc aagtgacgtt
    10801 ttaatgctgc agaacgttct tcttttgttt tgtataattt tttgattgct ggaatcatag
    10861 aaaatgccca gccaccgttt tgcatacgtt cgtagttcca tgaaccttga atgaaggtag
    10921 agcgccacgc aacagctaaa cgatcttttt ttgataattt gattttttct tctgccattt
    10981 tattttcctc cttcaagatt aatagtcatt caaaatgtcg cctaaagggt caccagagtt
    11041 gcttccgcct ccattatttg atgaaccacc cattttagaa aggttcaagt aaataagagc
    11101 taatgcaaca cctaaagcac ctagagcaat caatgttaat tgagaaattg cagctacaac
    11161 gaaaccaata acgaagaatg gccatacttc ttttgtagcc atcatgttga ttaccaaagc
    11221 gtaacctaca gctacgacca ttcccccacc gatagccata ccatctgtta accatgctgg
    11281 cattgattca aggaatgatt gcacagtttg tgctgggata aatagcaacg ctgctgctgg
    11341 gaccgcgata cgaacccctt gtaaacatac agcaatgatg tgccacattt caaccttacg
    11401 gatgtttcct tcttcagcag ccgcatccat caaatgaacg attggaacag cgattgtacg
    11461 tacgatcatt gttaagaaca accctgcaac agccaaagga acggcaatcg cgatcgcaga
    11521 aggaacacct ttaacacctt gtccacctaa aactaaaata attgctgatg ctactgatgc
    11581 taatgcagca tctggtgcta cagcggcacc gatatttgcc caacctaaag cgatcatctg
    11641 aagcgtaccg ccaagtacga taccagcttc gaggttaccg gtaactaaac cgatcaatgt
    11701 acatgccact agtggttgat gaaattgaaa ttcatcaaga ataccttcca taccagctag
    11761 aaaggcaact aatacgacta aaattattga aataatagac atgataagcc tcctataaat
    11821 tttaatatta tttattacgc ttttgctaat tcagcttttg cttttttcaa aatatcatcc
    11881 atgttatcgc gtgaatcatt aggtacttta cgtacatcga atttcacacc tttttgttca
    11941 agcttctcaa acgcttcaac gtcttcttgt cccatagaca atactttact tacaacgact
    12001 ttaccgaccg aatgcgccat tgacccaaca tttaattctt tgatgtctac gccaccttca
    12061 acagctgtca atacgtcttc aggattttca aataaaagca atgctttggt atttccaaaa
    12121 cgagggtctt ttgctacttc gatcattttg ctaataggaa taacatttgc ttttacacct
    12181 ggaggagcag cttgttcgat tagtctctta cgcagatcat cctttgatac tgcatctgaa
    12241 acaacgataa tacggtttgg ttgcactgct tttgtccaag ctgtcgcaac ttgtccatgt
    12301 aacagacgtg agtcaacacg tgctaataca tatttgattt ttccgtctcc gaccacagtt
    12361 ccttctggaa gtgctccttt aggttgtgcg tcttcaactg ctgctttcgg tgcttctgcc
    12421 ggttctaatt cttccggttt tacttttaca ccgtcttttg ctgtttcaat gatgtgtgct
    12481 gcaatttcat gagcagattc cattgaaaag cgtgatgcgt aggcttcaat caacattggt
    12541 aaattcaaac cgctgacgat tgcccattta tctttgtgct cttcgaataa tgtgtttgat
    12601 tgattgaacg gtgttccgcc ccataaatca actaagaata acacttcgtc ttgattatca
    12661 aaagaagcaa ccgcttcttc caatttggct ctcaagtcat ctggaccttc acttggtttc
    12721 aaaatcacgg ctttgacgtt ttcttgttcg ccaaagatca ttgaaccgga ttgtaagatt
    12781 ccttcagcaa attgtccatg acttgctagg ataatcccta cca
//
LOCUS       DACESX010000079        12735 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.079, whole genome shotgun sequence.
ACCESSION   DACESX010000079 DACESX010000000
VERSION     DACESX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.079"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            58..2781
                     /locus_tag="IXF88_001839"
     CDS             58..2781
                     /locus_tag="IXF88_001839"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012385.1,HMM:NF013070.1,HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1508569.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            2909..3331
                     /locus_tag="IXF88_001840"
     CDS             2909..3331
                     /locus_tag="IXF88_001840"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose IIA subunit"
                     /protein_id="HAQ1508570.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            3349..3819
                     /locus_tag="IXF88_001841"
     CDS             3349..3819
                     /locus_tag="IXF88_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1508571.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            3843..4625
                     /locus_tag="IXF88_001842"
     CDS             3843..4625
                     /locus_tag="IXF88_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1508572.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            4615..5439
                     /locus_tag="IXF88_001843"
     CDS             4615..5439
                     /locus_tag="IXF88_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1508573.1"
                     /translation="MTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMMPA
                     LEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTITS
                     IKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLRYF
                     GVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQGDE
                     ATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT"
     gene            5474..6472
                     /locus_tag="IXF88_001844"
     CDS             5474..6472
                     /locus_tag="IXF88_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1508574.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            6489..7343
                     /locus_tag="IXF88_001845"
     CDS             6489..7343
                     /locus_tag="IXF88_001845"
                     /inference="COORDINATES: protein motif:HMM:NF012515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508575.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(7917..9167)
                     /locus_tag="IXF88_001846"
                     /pseudo
     CDS             complement(7917..9167)
                     /locus_tag="IXF88_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            9684..9767
                     /locus_tag="IXF88_001847"
                     /pseudo
     CDS             9684..9767
                     /locus_tag="IXF88_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9856..11267
                     /locus_tag="IXF88_001848"
     CDS             join(9856..10411,10411..11267)
                     /locus_tag="IXF88_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1508576.1"
                     /translation="MAKYPFELKLKIVKEYLSGVGEYRHLANKYGISSKSQVSDWVNA
                     YREYGEEGLLKKHQNQKYSVQFKLNALELYQTSEMSYREVASTLEMNNPALIANWMRN
                     FREAGIEGLSKEKGRPLTLTRKKENKKENTTTEERERIKALEKQVRSLQIENAFLKEL
                     RKLRKQEAQQRRKNQSHESSPASEDPFKLVELLETLKFPKATYMYWQKRFDRPNPDQE
                     IEEKMLEIRKEHKDYGCLRLKKELENQGIHVNKKKIQRLIKKLGIEVKSYTRKSRRYN
                     SYHGKVGTVAKNRIHRRFYTNICHQKITTDTTEFKYYEVDTTGVVRQKKLYLDPFMDF
                     YNSEILSYRISEKPNALAIMEGLEEAIQMTNDCPFRRTFHSDQGWAYQMNAYKNKLKQ
                     HKIFQSMSRKGNCLDNSPMENFFGLLKQEIFHGEVYRSLDELKTKIDQYIYYYNHKRI
                     KKKLNWRSPVQFREAVKTAV"
     gene            11342..12625
                     /locus_tag="IXF88_001849"
                     /pseudo
     CDS             11342..12625
                     /locus_tag="IXF88_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4259 a         1990 c         2567 g         3919 t
ORIGIN      
        1 gaatattgaa ttttatagtg aaaatcattt attattaagt aaaggaggtg ggttcatttg
       61 aaaacgaaaa ttttaaaaga attaaatgat ctacttaaca gctatgattt tccacaatat
      121 tttccagaag aatgtaacgc gcaatatatt gcccaaaaat ttcaaataaa aagaaatacg
      181 gctagtaagc atctaaacga actaatggaa cagaaaaaag ttgtgaaaat caatggtcgt
      241 cctgttcttt tttttccagt ccatacaatt gaacggtata tatcacattc attagatagt
      301 tttgagtttt cttcaattga ttcgtttaga gaaaaaattt caaagaaaaa agcagatcat
      361 ttttttgatg atttaattgg tgcaaaacaa agtttaaaag aagcggttca acaagggata
      421 tcagcaatga aatatccagg tggattacca ataatgtatt ttggatcgtc cggagttggg
      481 aagagttact tagtggaaaa actagctgag tattgccgac aaactggtat tattcatgag
      541 aatgctccgt tcatcgagtt aaactgcgct cagtatttta ataatccaga gttattaaca
      601 agtcagttgt ttggccatat gaagggatcg tttaccggcg ccgattctga ccatattggt
      661 ataatagaag cagcagacgg tggaatcgtc tttcttgacg aaattcatcg tttaccacca
      721 gaaggacaag agaaactttt tattcatatg gataaaggcg tttttcgaag agttggagat
      781 agtggtcctt ggcgtttatc acatgttaga tatatttttg cgacaacgga acaggaagaa
      841 aatttttttc tagaaacttt tcgacgaaga attccgattg tttgtacttt gcctaattac
      901 caagatagag aaattagcga acgaaaagag attgtcttcc atttattaga gaaagagtct
      961 actctgatca ataaagaaat attagtttct gaagccgccc ttagtttcct gattcgctta
     1021 gatttgtatg gaaacattgg tgagctggat ggactaataa aacaaactat tgcgaataaa
     1081 ctatttagtg gaagcaataa aaaatatatc gaaattcata tttctgattt acctagcagg
     1141 tacttgctaa cagaaaatat tgagttaaaa aaccaaattg cgagccaacg gttctatatc
     1201 tttagtaatg gtaactatgt tcatgattgt gacgagactt caactgtaac tacaaattta
     1261 cgaggtttga ttcatcgtgt ttctaaatat tattcagagt gtcatgatgt gaatagaaat
     1321 agtgaagagt ttcgacgttt tgtgaaggac caaatggcag aatttaatga tcgttttatt
     1381 taccaaaaaa attcagaagt cttagttaag tattatttac cggaaatcca aaatattgtt
     1441 caaaaaatct ctcccagtct ttttgttcac ctttctggca atacggtcca agctatagct
     1501 acctatctcg ctattaggga tcaatggggg tatgggaaag agaatgcttc agcgaatgaa
     1561 attgaaaagt tatatgaaaa cctaaagtgt aactatttga cggatttaaa tgtattctta
     1621 ctgatcttcg agaacgccct ggatacaaca ttgactattt gggataaact gtatatcagt
     1681 ttgtgtgtta ttagtgagac cggaaatgaa gcgaaagaca ttaacgcaat tattttagcc
     1741 catggatatg cgacagctag tagtattgct aatgttgcca atcgtttggt taatcagttt
     1801 gtcatagatt ctattgatat gcctctagat atcagcttta gtgaagtaat tgacagatta
     1861 cttcactata tagagtatcg aaagcctaaa gatggactag tgattttcgt tgatatggga
     1921 agtttagctc aaattaagac tgaaattgaa caagtaatcg aagttccaac aatgattatt
     1981 aataatgtta caaccgaaat ggctattgaa accgcacaac tgattcaaag tacgagtgat
     2041 atccaaaagg ttgtaaaaaa attgccgtat tctcagtttg aaaagcaagt tctatatccc
     2101 atcaaaatta gaaaacgtac aatcgttgtt tcctgcaata ctggtttggg tacctccata
     2161 aaaattaaag aaatgatgga aaataatttg tcgaaagact tgggcattga atttctgcca
     2221 tacgaaaatg aaactcttag agatactcag caattagagt ttctcattaa aacaaaggat
     2281 atcattgcca ttatcggaac agatgatccg cgtgtgtcga acattcccta tatttctctt
     2341 gaagaattat ttgatggtga aattggcaaa ctctataaag ttcttttgag agtctatgga
     2401 aaagagattg ctgaggcaat taatcaaaat attgttaaaa atagctcatt aaaccgcttg
     2461 attgacagtc ttactatttt agatgccgaa aaagttgtca atcaaattga ggaaagttta
     2521 aaagattatc aaatgagggt ggggaaaacg ttatcatcat tgtccaaaat taatctttat
     2581 gttcatatca gctgtatgat tgagcgacta attcgtaatc agggagtaag tagttatcca
     2641 gcaatagatc agtttcttaa gcgatacgaa actgaggttg cttctattca aaaggcactt
     2701 agtaagattg agaaacttta taatattaag attcctatgg aagagattgc atatgtgtgc
     2761 aacatcattg taagttattg ataaaataaa gtgacactat tcacagcgat ggaaatagtg
     2821 tcactttatt ttttgtacgc cctttcattt aagtggtgac agtaattggc acagtatttg
     2881 ctaataacaa agcgaaagga gttcgggtat gagacaaata ttatgtatga gccacggtcc
     2941 tctagcaaaa ggaatgattg aaactttgag tattatcgta ggagatttgg ataatattga
     3001 ttttcgatgt gcctatgtcg atggaaataa tgacgtagaa atgatcatcg atgattatct
     3061 aaaggaatct atgggaaatg agacgattgt tgtaaccgac atttttggtg gaagcattaa
     3121 taatgagtgg ctaagacggt tgccagatct ttctaaagtc tatttgattt ctggaatgaa
     3181 tttaagtctt ttagtggatt tatatttaaa attccaatta gtttcatctg atcagattga
     3241 agaaattatt gatcatgcaa ttcaagcatc ttctggttcg attaagtttt gtaatcagct
     3301 agattatgag caaacggttg atgactttta aataaaggag ggaagagaat gattaaatta
     3361 gtgaggatcg atcatcgttt gatacacggt caagttgctt taacttggac gaacgcaatc
     3421 agtgcggatt gcattttagt agcgaacgat gaaattttga atgatccaat tcaaaagaca
     3481 actttgaaat tagccgcacc acataacgtg aaggttgtta tcaaatcaat tcaagattcg
     3541 attgatgcaa ttaatagtgg gaagacagat aaatacaaac tatttgtagt taccaaaacc
     3601 atttccgatg ctaaacggtt ggcaaatggg gttgaacaaa ttaaatctgt aaatatcggt
     3661 agtgttaagg aaactagtgg cagtagcgct ttgacgcata caatttttgt aacagaagag
     3721 gaacgagcag aacttaaagg aatggtctca gagggcattg aagtgttcgc tcaacaagtt
     3781 ccaacagata aggcaataga tttaaaaaaa tctttgtaat aaaaaaataa gggggtaatt
     3841 tcatggatag tctcttagta caagcaattt tgcttggtct cttgactgcg ttcatcgtaa
     3901 ttgactggat gacaggtacg aataacttat caagaccaat cgtatcttcc gtctttgttg
     3961 gtttaattat gggcgatctt aagatgggga ttatgatggg ggcaacactg gagttagcat
     4021 ttatcggagc aataacaatc ggagcggcac gtccgcctga tacggtatct ggtgggattt
     4081 tagggactgc gtttgctatt gcaactggca agggagcaga atttgcgttg accttggcat
     4141 tcccaatcgc tgcgctattc ttattagttg ataatctact aacattgctt gttctaccaa
     4201 tgattgctag aaaggcagat agatatgctg aaaagggcga actaaaaaat gtgtcgaaaa
     4261 tgcatgtttt aggttttatc ttagtaaaaa gtttaccacg ggctttattt gtcggattct
     4321 cattttactt aggaagtcct gttatggaac gaatcttaaa tgctattcca acttttgtcc
     4381 aagatgggat taacattgct gcaggcatta tgccagcact aggttttgct attttattgc
     4441 aaatgcttct taagaaagat gtctttgtat atttcgtaat tggttttgca ctctttagct
     4501 acttacatgt gccgattctg gggattgcaa tcttcggcgc atgtctagct tatattatag
     4561 taaacatgga aaccaaagct gcaatgggtt caagagggga ggagatggtt gaagatgact
     4621 tctagtggga aagctttaac taaagatgat ttaacaaaaa tttgtattcg gtcatgtgca
     4681 ttagaagcat catggaattt tgagcgccaa caacatatgg gctttgcttt tgctatgatg
     4741 ccagcgttag aaaaaattta tagtggcaat gaagaagaaa agcaaaaagc agctcaaaga
     4801 catctagagt tttttaacac agctccgcca ttatcaacat tcataatggg aattgtggct
     4861 tctatggaag aagaaaatgc caaaaatcct gatgactttg atacttctac aatcacttct
     4921 attaaaactg cattgatggg accattagct ggtattggtg attcaatgtt ttggggtacc
     4981 ttattagtta tcggtatcgg tgtaggtgct tcattagcaa aagaaggcaa tattttgggt
     5041 ccatttttat ttttactaat cttcaatgtc cctaattttc ttctccgtta ctttggagtt
     5101 atgcagggat ataactttgg tacaaagcta ttagagaaca ttgaatcctc tggcgtcatg
     5161 aaaacgttga ctcatgctgc ctcaattttg ggtcttatgg taattggggg catgtctgga
     5221 agtatggtat caatcaaagt agctggttct attggacaag gtgatgaagc aacaaaaatt
     5281 caaagtatca ttgatggtat tttaccaaat ctgctgtcag tattatgtgt atttctgatt
     5341 tactggatgc ttaaaaaagg ctttaaaaca acttacgtgt tattcggaat ctttatcttt
     5401 ggtatcatag cagccttctt cggatttttg acaacttaat tgaataatct aaataattgt
     5461 aaggagagaa ctaatgttaa actataatgc aaaagaaatg aaaaaaaaag gcaaacggat
     5521 ttacggtatt cgtgaagatg tagaaaaaat tgcaaagcaa gcttatgatt ctggaattag
     5581 taatattttc tttactgctt ctggtggatc ggtagccgta atgcaaccat ttgagtatgt
     5641 attgatgact gaatcaagta ttcctgttta ctctatgact gcagctgagc ttgtcttaat
     5701 gggacataaa cagttgaatg aaaaatcttt agtaatctta tactcaaaat ctggagatac
     5761 gaaagaaact attaaggcag ctgaatattt aaaagaacga aacgtgccaa ccgttggtat
     5821 tgtaagtaaa gaagatacac cgttagaggc tctatcaact tatgcaatca attacctaga
     5881 cggccgccct caagaaatgc cactttattt cttaattatg tctatcatga atttgaatgg
     5941 ggaatttcct gaatacactc gttttgctga tgaactgaaa aacttaccaa cggctctgaa
     6001 ttcagttaga gaacaggctg atgaaaaagg gaagaagtat gccgaagaat ttaaagaaga
     6061 tccttatcaa atttggattg gtagcgggaa tttatgggga atcacctact cttattcaat
     6121 gtgtgtgctt gaggaatctc aatggttgcg tacaaagtct gtctcatcac cagagttttt
     6181 ccatggtacc ctcgagttag tcgaaaaagg agtttgtgta acacttttga aaactgaggg
     6241 ggaaacacgg cttttagatg ctcgagttga aaaatttgcg gtccaatata cagataaatt
     6301 taatgttttt gatactaagg attatgagct accaggtatt agtgacgaat ttcgaaagta
     6361 cttttgtgct ccagttatgt gggcagccct aggtcgcgtc agtgttcatc ttgaagatat
     6421 tcggaaccac tctttggata aacgtcgtta ttaccgagta gttgaatact aaaagtaggt
     6481 gattaattat gacagcgtat gtgattggat ttggcgataa tgttgttgat tactatgaga
     6541 atttgaatca aaaatttcca gggggaaatg cagttaattt tgctgtgaat gcaaaaaaga
     6601 gtggtgttaa agcatactat gtcggtagtc tatcatctga tcctgatggc gtgttattac
     6661 aacacgccat cagttctgaa ggtgttgata tctctttctc ggaagttatt gacgagccta
     6721 ctgagcaagc acatgtaact attaaagatg gagataggga gtttataggt ggcgtgcgtg
     6781 gctcccgatt gacacctgct ctctcagcag aacttattga tttgatgaag aacagttcat
     6841 tagttcatac tagttgccat agtcgaacag aaacaaaaat taaagaatta aaaaaatatg
     6901 atgttaaagt ttcttttgat ttttctgact tagataaata tagaacgcat gaatacttgt
     6961 cagatgtttg tccgtcaatt tatattgcac agttttcagt cgctacagat gatcaagagg
     7021 aaattaatcg cttaattagt gaatgcaagg actttgaggt tccgtatctt ctttttacgc
     7081 gtggtagtaa gagccctgtt tttgttgaca ctcgagagaa taagacttat gaaggattca
     7141 ttcgcaaagt tgagtcacct catgatacaa tgggcgcagg tgatgcatac tttgcagctt
     7201 ttgcaagtaa ttttgtctta tctaaacgtg aaaaatcaat gatagaacat gttattgatt
     7261 gttttcacaa aggtgcaaac cttgcatatg aaacattgat gattgatggc tcctttggtc
     7321 atgctacacc tattaaccct tagctttgtg ctttgggctt ttattgctgg aatttgtata
     7381 gtagactcat cataactata tttttgtatg tgaaaaggca agaggctatg atgaaaataa
     7441 cactggctaa ttgcatgagg gagcatatcg ttgttaaagc gtcattgata gtatgaataa
     7501 atatctcgat aatattatat tgttatttac ttaatttatt gattagtcaa gcaaagataa
     7561 tcttctgtta cttaacgaga ctagtaggta caagcacttc atgttatgta cgacccaaaa
     7621 atattgctgt tgattatttg ctgaaattat tgcgagcttc aatttatttt taaaggactt
     7681 ggtaggctgc gtgatgaaat aagacttgga aggctgtcta aaccaatata tagtagacta
     7741 aatagtaaaa aagagcatgg taattgtctc tttgtcaata agtctaatgg taataagaca
     7801 agttggagat tgagaatatt tttattgtgt aacctatttt gaagcgtact taactagccc
     7861 cggccaaaat caaatttttt cggggtatat caagggtttg cgtcaataac agaatgttat
     7921 ttatctatca cgttccttgg tggcgtgtag agttgatggt tacgtagtag cgtatctacc
     7981 agacgcgtaa attttcttgc ggttaagacg tgtgctcgtt tgtgtttatt tttgggacct
     8041 ctttacattt gctttgataa aaagctttat attgaggaag atattttttt acagagttgg
     8101 aagcttcaac taagtagtat ctaaaatagc gactcctttg ttttgtcaga ggtgtatttt
     8161 gtgaccgata gttaacagat tgtttgcctt tccaacataa gcctgcgtat tttgccaatt
     8221 ttgtttgatc ttcaaatcgt tcaatttgac cgatttcagc gattaatcca gcggcgtgta
     8281 cctttccaat acctggaaca ctagtcaaat attgatattc tggaataacg acaacaaatt
     8341 gctcggttgc tttatccaag tctttgatgt tcttttcaag cgcacgaatt tcacatacaa
     8401 aaacatttag aacgacattg atggattctt atgcaagact atctaaccga taactcacgg
     8461 caattgcacg ttgaatcgat ttggcatttt tttctggatc tttaaagcga catttccttt
     8521 ttcttttagt aagtcacaga aagcttccaa aggaagcaca gccaattcat ctagcgtgaa
     8581 gtcttctgtc acaagagtaa tgacagtggc actatagaga cttgtcgatt catcgcgcat
     8641 ttatcgagta agcgtattgc atttgtacgt tagattttcc agaaagtgtt gttttgtacg
     8701 agtgaagttg cttaatgagt cgatagcgag tgcgtgttaa acgctgtaaa gccacgtact
     8761 tctcttcttt gataggagaa gttgtgaacc gttgaatacg caataaatca gcaattctta
     8821 acgcgttgtt tcgatcagtc gtatcttcct caaacatgcg actgaattgt ttcatccgaa
     8881 aaggattttc aatcctcaca agtgcattga gtttttttag ctctgcatct tcatggaaaa
     8941 acatcgaagg atgaaagctg tacatggacg tttaattcca tctcaataac gatttgaccg
     9001 aagtgattgt tttcattgaa cacaagaatc agttctcgtg tcaatggagc cctttcaatg
     9061 tcattagcaa cagaattttc agtcaatata gatagctgat cgtcgtctgt taggaagcaa
     9121 acgtctaatt tttcagaact aacgtcatta ccaacaaata atttcatgga ataaaaacct
     9181 attttcgata taaattctag aaaatgcttc gatactgtgg gattccacag aaactcactg
     9241 tgtggtcacg acctcgccta atagaatacg gtaataaaag agctaatgag ttgatagtaa
     9301 acacagcgct ttggataaca gacttatgaa ggtagacaaa gctacaggac tgattagcaa
     9361 attctcgttg aatcctttcc atacatccat tgtactctga gaaatcacac aataacgaaa
     9421 taaattccgg aaggaatatg ttattcaaaa aaatttgtgt tttatagata ctttaaaaga
     9481 aacttattag gcaaaatata ttttacgaag actgatgagt tgtgcaaaat caaatctgag
     9541 tcagaatgaa cttcattcta gctcagattt ttgtttaatg acttcagtca gtttcttgca
     9601 ttacgtgaat gaaaatggga ctggtgatgt ccaaacccta aaaagttaga ccaaaatcta
     9661 acttcagggg ggatggtatt tttatgggaa aatatagctt tgaattcaag tcaacaatcg
     9721 ttcaagagta tcttgaagga gaaggtggat taggatattt agcaaaaata caaggagtga
     9781 gatcaaaaaa aggtacacgg atggatcaat caaaagttag accaaaaaat ctaacttttg
     9841 ggggtcgtta tttgtatggc aaaatatcct tttgaattaa aactaaaaat cgttaaagaa
     9901 tatcttagtg gcgtgggcga atatcggcat cttgccaaca aatacggtat ttcaagcaag
     9961 tctcaagtaa gtgattgggt gaatgcctat cgtgaatacg gagaggaagg tctattgaaa
    10021 aagcatcaaa atcagaaata ttctgttcaa tttaagttaa atgcgctaga gttatatcaa
    10081 acaagtgaga tgtcctatcg agaagttgcc agcactttag aaatgaacaa ccctgcgctt
    10141 attgccaatt ggatgcgtaa tttcagagaa gcaggtattg aggggctctc aaaagagaaa
    10201 gggcgaccac ttactttgac tagaaaaaaa gaaaacaaaa aagaaaatac aacaactgaa
    10261 gaacgcgagc gtatcaaggc attagaaaag caagtccgat cccttcaaat tgagaatgcc
    10321 tttttaaaag agttgaggaa gctgcgaaaa caggaagccc agcaaagacg caagaatcaa
    10381 tcgcacgaat catcgccagc ctccgaggat cattcaaact agttgagctt cttgaaacat
    10441 tgaaatttcc taaagcaacc tatatgtatt ggcagaaacg gtttgacaga ccaaacccag
    10501 accaagaaat tgaagaaaaa atgcttgaaa ttcgcaagga gcataaagac tatggctgtc
    10561 tgcgactaaa aaaagaatta gagaatcaag ggattcatgt caataaaaag aagattcaac
    10621 gtctgataaa aaaactaggt atcgaagtaa agtcttacac tcgaaaatct cgacggtaca
    10681 attcttatca tgggaaggtt ggcactgtcg ccaaaaatcg tattcatcgt cggttttata
    10741 caaatatctg tcaccaaaaa attacaactg ataccacaga gtttaagtat tatgaagtag
    10801 acaccacagg tgtcgttcgt cagaaaaagc tgtatttaga tcctttcatg gatttctaca
    10861 atagtgaaat tctatcttat cggatttctg aaaaaccaaa cgcactagcc ataatggaag
    10921 gattagaaga agcaatccag atgacaaatg attgtccatt tcgtagaacc ttccattcag
    10981 atcaaggatg ggcctatcaa atgaatgctt ataagaataa attgaagcag cataaaatct
    11041 tccagagtat gtctcgtaaa gggaattgtt tagataactc acctatggaa aacttctttg
    11101 gtttattaaa gcaagagatt tttcatggag aagtctatcg gagtttagat gaattgaaaa
    11161 caaaaattga ccaatatatc tattattaca accataaacg catcaaaaag aaattaaatt
    11221 ggcgaagtcc ggttcagttt cgagaagctg taaaaacagc tgtctaacag ttaattttat
    11281 cgtacaaaaa aggagtggat ttctccactc cataaaagtc taactttttg gggtcgctac
    11341 aaatgcttat cgagaatttg gagaagaagg cctcttgcgt aagcgaaata agcgaagtta
    11401 ttctgttcaa ttcaagatag atgcgataga gttataaaaa acgggtgagc tatcctatcg
    11461 tgaagtggcg aatcatttag ggataaacca acctgcgtta gttgtcaatt ggatgaaaat
    11521 ttttgttcaa aaggattgca cgggctctca aaaatgaaag ggtcctcctc cattttgtct
    11581 aagaaagaat aaaaggaaga aacaagcaaa ctgacgccta aagagcataa tcgtatcaaa
    11641 gaattggaaa aataagtgcg tttccttcag attgagaata cctttttaaa agaattgagg
    11701 aagcttagaa aacaggaagt ctaacaaaga cgaacgaatc aatcgcacga atcatcgaca
    11761 acctccgagg atcattcaaa ttagttgagc ttcttgaaac cttgaaattt cctgaattaa
    11821 cctttatgta ttggcaaaga cgatttgatc gaagaaattc agatcaagaa atcgaagagg
    11881 aaatgcttaa aataagaagt caatataaag attatggcta tttacgtatg acaaaggaat
    11941 tgtgggaccg tgggttccat gttaacaaga aaaaagtaca acgtttgatt cgaaagctga
    12001 gtattgaagt acgatcattt acgagaaaat atcgccgcta taatacttat cgtggaagaa
    12061 tcggcatgat tacaaaaaat cgaattcatc gacgctttta cacaagcaac tgtcatcaaa
    12121 aggttacaac agatacgaca gaactaaaat attttgaaat ggataaatac ggtgccaatc
    12181 gtgaaaagaa gctgtatctt gatccatttt tagatatgta caacagtgaa attttatttt
    12241 atcgcatttc tgaaaagcca agtgcgttgg ctatcatgga agctttagag gaagctatct
    12301 gcgcaacgga atgtatctat cgtcgaactt ttcactccaa aaaggatgga cctaccaaat
    12361 gaaagcttat agtcataagt tgaaagaaca taagatcttc caaagtatgt cccgtaagga
    12421 gacctgctta gataattcga tcattgagaa cttccttagt ctattaaagc aggaaattta
    12481 ttatggaaga gactatcgta tctttgaaga attgaagcaa gccattgatg gctatattca
    12541 ttactacaac catgagcgta tgaaaaggaa actaaactgg cagagtctgg tgcaatttag
    12601 agtaatagca ttcaaagctg tctaaaatca tcaagaaaag caaagaaaaa cggagtgaat
    12661 tttctccact ccgaaaacat ctatcttttt taggtcacta cagaatcaat tctgattcat
    12721 cagacccata aatta
//
LOCUS       DACESX010000080        12705 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.080, whole genome shotgun sequence.
ACCESSION   DACESX010000080 DACESX010000000
VERSION     DACESX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.080"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(334..696)
                     /locus_tag="IXF88_001850"
     CDS             complement(334..696)
                     /locus_tag="IXF88_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508577.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="IXF88_001851"
     CDS             complement(770..1150)
                     /locus_tag="IXF88_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1508578.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1232..2407
                     /locus_tag="IXF88_001852"
     CDS             1232..2407
                     /locus_tag="IXF88_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1508579.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2484..3068)
                     /locus_tag="IXF88_001853"
     CDS             complement(2484..3068)
                     /locus_tag="IXF88_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1508580.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHI
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3269..4267
                     /locus_tag="IXF88_001854"
     CDS             3269..4267
                     /locus_tag="IXF88_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1508581.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4379..4888
                     /locus_tag="IXF88_001855"
     CDS             4379..4888
                     /locus_tag="IXF88_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1508582.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4918..5577)
                     /locus_tag="IXF88_001856"
     CDS             complement(4918..5577)
                     /locus_tag="IXF88_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1508583.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5579..6343)
                     /locus_tag="IXF88_001857"
     CDS             complement(5579..6343)
                     /locus_tag="IXF88_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1508584.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6351..7028)
                     /locus_tag="IXF88_001858"
     CDS             complement(6351..7028)
                     /locus_tag="IXF88_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1508585.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7065..8450)
                     /locus_tag="IXF88_001859"
     CDS             complement(7065..8450)
                     /locus_tag="IXF88_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1508586.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8660..8744)
                     /gene="ffs"
                     /locus_tag="IXF88_001860"
     ncRNA           complement(8660..8744)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IXF88_001860"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="IXF88_001861"
     CDS             complement(8767..9282)
                     /gene="tadA"
                     /locus_tag="IXF88_001861"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1508587.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9389..10246
                     /locus_tag="IXF88_001862"
     CDS             9389..10246
                     /locus_tag="IXF88_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1508588.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10327..10662)
                     /locus_tag="IXF88_001863"
     CDS             complement(10327..10662)
                     /locus_tag="IXF88_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508589.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10729..12099)
                     /locus_tag="IXF88_001864"
     CDS             complement(10729..12099)
                     /locus_tag="IXF88_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1508590.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12177..>12705)
                     /locus_tag="IXF88_001865"
     CDS             complement(12177..>12705)
                     /locus_tag="IXF88_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1508591.1"
                     /translation="RVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYPIYT
                     HFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAKSDL
                     DMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWDKNE
                     VTDEYLENILKEQSP"
BASE COUNT         3780 a         2571 c         2123 g         4231 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta tatgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tgtttcctcc taatatttgc atacttcata ataataataa cataatacct
     3121 aacctctcgt aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta
     3181 taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct
     3241 gctatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc
     3301 aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat
     3361 cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat
     3421 ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa
     3481 acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg
     3541 tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat
     3601 tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc
     3661 atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa
     3721 agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat
     3781 tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt
     3841 atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc
     3901 aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat
     3961 tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct
     4021 ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac
     4081 ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact
     4141 gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa
     4201 tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc
     4261 tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt
     4321 tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat
     4381 ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt
     4441 aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt
     4501 gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa
     4561 cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc
     4621 gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt
     4681 gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt
     4741 gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt
     4801 agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc
     4861 agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta
     4921 gacgagttct ttcttttttg tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc
     4981 aaaaaataat tctaataata caaaaaataa gataaagcta tgcatgaaaa attcttctgg
     5041 aagtacattg atgattggat cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa
     5101 cgtttcatga aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac
     5161 aactggcagt aacatgccga tttgaaacgg acgaatcaac cgccaaagcc gatcattctt
     5221 ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca cagcataatc
     5281 cactaaaaac aaaattttca cttcataaaa gtgatgggct ccgctagcag aaactgggaa
     5341 atctggcaag ctgaggatgg ttttgaaggg attattgaga taattcatta ggtgatcaaa
     5401 atttttcagt aacgtctctt gatctaaaga agtataatcc aaaatttgca aatgtccgat
     5461 atcaaatacg tatagtggtc gaaagttgat cgtcacacca actgccagac tgatcagtgt
     5521 cagaaatagg ctgaacaatc cagcatattc catccactgc catctcgaat ttttcatatt
     5581 agaagctcca ttcatccagt gattgtttca cataggtagc tgggataggc aacccaggta
     5641 catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt atctatatta ttgcgtatcc
     5701 cagcttggat atctgtttca tagttatctc caaccatgat tacttcttct ttttgcagcc
     5761 ctaatacttc aacggcttta tccataatga tggcttctgg ttttccgatg tagatcggcg
     5821 gcgtttgggt tgctgtctct acaaaagaga tgactgaccc tgctccagga agaaggccgc
     5881 gttcagtagg tatattctta tctgggttcg ttccaatgaa agtagcacct ttttgaatag
     5941 caagtgttgc tttaactact ttttcatagg tcagataatt atctaagcct accactacat
     6001 agtcaggagc ctcttcttcc catgtaaatc cggcagctaa aatcaagtca atcaatcctg
     6061 cttctccgat aacatagact tttttccctt ttccatctgc tttcataaaa tcgattgttg
     6121 ccaaagtagc tgtgtagact gtctctggag ctacatgaat gtcaaactca tctgctagac
     6181 gattagctac tgtttctggt gacttagtcg tgttattggt aacaaacaag aaaggtaatt
     6241 ttcttttttg caattcttcc acaaatcgtt taccggccgg tatgggttgt gtcccacgat
     6301 aaatcgttcc gtctaaatca attaaatatc ctttatagtc cattcatcca ctactcttcc
     6361 ctttttctga tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg ttcttgtttt
     6421 tttacttttt cagaggtttt tgctgactct gtttttcgat ttttaataac cggttttttg
     6481 cgattagatg cgactggtcc ttttttctca tcgatatacg cacgattatg gttttttttc
     6541 ttttttcggc gctgagttgt cttttctttt ttaccgccga ttctctcgat gacaaaatac
     6601 gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct
     6661 ggatgcgccc gtcgattatc cgcttggaaa aatcctttta ggcgtagttg ttcataaccc
     6721 caatctccta cgatataatc atatcgggct agtacatcac tgtaacgttc tccaagcttt
     6781 tctgcattaa atccttcacg ataatcggac acaagccgat aatgtctttc acctatcaaa
     6841 aaattactgt catctaacag tgtcactagc tcgcctttga ctttttctgc tttaggctcc
     6901 tcaacgattt cttccaaagc agctgtcagt tcttcggtga ctgttgtttc ctgttcagct
     6961 gatgattttt tcttagcaat ttctgtttta tgctcaacgt tctgctgttc attttgtttt
     7021 gcagacatgc gttcaccacc tatctttttt attatacctt gtttttattt gattgggtag
     7081 tgagctttca agtaatctcc taccacactt cggataaatt cagggaacaa aaattcattt
     7141 tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta
     7201 aaagtataat ttttattcag atcgattggc ttgttcttcc aaaagacttg atggttaata
     7261 gaatcatatg tgattccatt atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt
     7321 cccttcatag gatagttacg taaaaagtta cgattttttt ctatttctaa aactaagcgg
     7381 atcagatcat ttccacccaa tgtgacattt aacaagtgca tcggatgcgg caacgcagtg
     7441 tgaagctgat cctgattgac ccgccctttt ggaatttcag ttaaaaacag accactgttt
     7501 aatattgctg cttccgtctt tcctcgttct ttcaccgcct caaaggctac ttggataaac
     7561 gaatgctcat tgaagatatc tagactcaga gaaaatggca aatctgctac ttctttttct
     7621 tttaataatt catgcccacg ttctagataa ctatttattt cttgttcatc ttctgtaaaa
     7681 gctgtcattg tttcggtagg gatggctttg gctgaatgct taaggatttt tttctcttca
     7741 tcgatggtta aatggacttc accgacatac tgcccgtact ttccagcggc tgctatttgg
     7801 acatcattga ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc taaaataaca
     7861 tcaattgctg gtatttcttt tgcaatttgg aaatcatcct gaatcccgag atgactcatc
     7921 aaaactaaaa catccacttg tgaacgtaaa tcttcgacta attctggcaa aatatcataa
     7981 ggattccgta tgtcccagcc attcggatta taagtaagcg gaaatggagc agtaaatgca
     8041 attagtccga ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc
     8101 ggtggctgga gcgtattttt atcgaataag ttatccagga tgatatcaaa ctcagcatga
     8161 tcgtataaat gattcaactc ttctttggaa tttcctacgc cttcattgtt tcctatcgtc
     8221 gctgcatcat aaccaacttc attcatcagc aagatatttt cctgtccatt cgttgcttct
     8281 gacaatggat gccaacgatc cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt
     8341 tctttctttt cgttttctcg tttacgttga tccaaaaacc ttctgatcct tggccaattt
     8401 tctaaatgag aatgcagatc attcgtatgt aaaatcacta ttttttccat gtaccctctc
     8461 gcctctcttt atctttatca taccataggc agctttttct tccattgaaa caagctctat
     8521 ttcagagttt ttcctgcctt gaagcaaatt gtataagtac aatattcgat aaatcagtct
     8581 ggctaaaaaa tcaggaacat tcatttttac agtactataa aaataaaaaa aacagacccg
     8641 aaagtctgtt gtcgtttcat tataatcaga cacatggcgg aacctgctta gtgctgcttc
     8701 cttccagacc tgacacgctt cacaagccca ccattgtcct agcctttcgg caaagaatag
     8761 tataccttat ttttcattcg ttgaccagtc tttttttaat ttcttttttc gctggcggag
     8821 ctttcggaaa aagtcagtca aaatcatccc acattcgtct tctaaaatgc caccttctac
     8881 atatgcttgg tggttaaacc gctcgtcctc caaaagattc atcaaggtac cggctgttcc
     8941 gccttttgga tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc
     9001 gctgcacata ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca agcgccagct
     9061 tcccactttt tcacaggctt ccttgattgc aagcatttct gcatgtgtag tagcatcttg
     9121 cgtggtttct ctaaggttat atcctcttcc aatcactttt ccatccaata caaccactgc
     9181 tccaatcgga acttcatgaa gcatttctgc tttctttgct tctgccaatg caaggcgcat
     9241 ccatttttct ttttctactg catcaagatt tgtatttacc aattaccctt ccctgccttt
     9301 gttttttctt gttttacttt agcatatgat aaaaaggctg taactaactt ttttttctat
     9361 ggtaaaataa atacaagagg tgaacaagat gaaaatggat aaattacttg ctctttaccc
     9421 acaagcacag aaaaaaaact ctccagcatc agaagcggat atcctttctt ttgcaattga
     9481 tgatgctttt atctggatca aacaagattc attatctcaa caagaaacca gccttttgaa
     9541 agcgttattc cctgtaatca acgatcaaaa aaaacaccca tggtatcact atctttttaa
     9601 agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa
     9661 aggagaattc ttaaaaaaag aatggcaaga tactattttg gaattatttc gctattctgc
     9721 tgatttcttt ttttatacag acacagatgc gctcttagtc gaacaaataa accgtgatta
     9781 tcttgatgct actgaaatca acggtatttt tctatcttta gatgctgatt ttgatacgac
     9841 aacgtctgta ttcgttggtt ctttccattc acctggcacc gacctcgtcc ctttgtttgc
     9901 tgaagaaaga aaaatttttt taacagagca aaacaatctt tatactcaca gccgtacttt
     9961 ttctatgcaa gatgtggctt tacattacta tacaaaagat actgtaaaag atagtaagtt
    10021 gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt
    10081 atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac
    10141 tctaaaatat cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa aaaaagccaa
    10201 cgatttactg ttttgctatc ttttattgct tcatgaacat aattaaagat agattaaagg
    10261 ggttgtgaca ataattttgt cacaacccct tatttcgaat gaatagtgtt caaaagctag
    10321 tttctcttac atcatttttt caacaaaatg cattagccgg tcttttacat cttcggttga
    10381 atctttgatt gaatcaccat aaacagagat tttctttttt cctgatttga tttttgctaa
    10441 cattcccatc aaagaatcca agtcactatc tgaaagacca tctacgattt ctagtaattt
    10501 ttcatttcca tcaaatttgt catagacgaa tttttttact ttcgtccgag tagtcatatg
    10561 atggatcttt tccattactt ttcccgatac aacgactgct gtcgctacac tggcagcggc
    10621 tgctacgctt aatccgattg ttacttttgt tgatgttttc ataagtatca gctccttttt
    10681 atctatctcc atcatagcat agacaaaaaa ataaacgaac taatccactc agtgaaatct
    10741 caatccttta gggaaaaagt tctttactgt tccttttact tgtttcccaa atccgacagg
    10801 tatcttatct aatactagca aaacccatcc ttgattccca tcacgttgaa atgtttctcc
    10861 tgctgtgtaa gattgccatt ccttttgagt gatcggcaag caaggtatat tctctatctt
    10921 ttttgtagca agcgctaatg catagctagg ttcaaatcga ttctttttga aatctcctaa
    10981 atgcaaacct gtacgtacga cttttaaacc atcgagagaa ggggctaact caggaacttc
    11041 ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct tcataatgaa aatcatttga
    11101 aaactccgtc cagagttttt cctgctcttt tgtcatttgc actttacttt tttttcgtgt
    11161 cttcttttct ttatgcatct gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc
    11221 ttcaccctga tctttatgag gccatatacg tattgttttt tccaaaccag ctacgcttcc
    11281 ccattcagaa cgtcctgaac taacggattg agtcaaagga atctcctcaa tcgttactgg
    11341 ataattttct acaagccagc taatgatctc ttcattttct tctggtgcaa acgtacaagt
    11401 cgaataaatc agttgtcctt tattttttag catttttatt gctgaagaca aaatctcttg
    11461 ctgacgtttt tgacagtata gcggagattc ttctgtccac tcttttattg catttggatc
    11521 ttttcggaac attccttctc ctgaacaagg agcatcaacc acgatccgat caaaaaagcc
    11581 cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc
    11641 ccatcgttct atattttctg ataaaatctt cgcacgtttc ggaaagatct cattcgtcac
    11701 taataaccct ttccccttca tttgagcagc taattgggtt gatttaccac cgggagccgc
    11761 acacaaatcc aaaacttttt caccgggttt tgctgcagca gctgttccaa cgatcattgc
    11821 acttggttct tggctatatt catacccagc ttgatggagg aaagactttc cattgacagt
    11881 gccaagaaat ccctcattgc tgtagggagc gggttgtaat tcttcgctat ggtaagtttg
    11941 gaccatgtcc aatcctgccg gttttagtgg attccaccga aatccttttt tcacgcttcc
    12001 ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac gatatttttt
    12061 gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt
    12121 ttacaatctt ttttacgaaa aataaaggtg gctgatctac tttttcatgt tgctgcctaa
    12181 ggtgactgtt cctttaagat attttccaag tattcatccg tcacctcgtt tttatcccaa
    12241 acagcgttca tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa acttcccaat
    12301 gcattaagcc ataccaattc aagcgacttt tcttctttga tcttcgtgtg acctttgaca
    12361 tatgagaaaa atggccgctt aaatagcgaa tccatgtcta aatcagattt tgcaatgatt
    12421 tcacgaatac tttcatggat agaaaaaaaa tctttcaata agtagacaat gcttaaaaca
    12481 tcatcggaac tgcattcagc taagtagttt tcaaaataaa cagtgatttc ttctaatatc
    12541 ttctctttta acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg
    12601 cagaactctg ctaactctct tgcagtaatt gacttcattc catattcttc cacgtatacc
    12661 atacttttga acagcaaata ttctttacta taatttgtta ctctc
//
LOCUS       DACESX010000081        12610 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.081, whole genome shotgun sequence.
ACCESSION   DACESX010000081 DACESX010000000
VERSION     DACESX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.081"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            16..870
                     /locus_tag="IXF88_001866"
     CDS             16..870
                     /locus_tag="IXF88_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018886003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1508592.1"
                     /translation="MGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQEVVAKKF
                     ILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSRKYLFGR
                     GFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRNFFQALP
                     DSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYINDSSKW
                     PIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQKYFVKG
                     ALVGSVKG"
     gene            complement(1328..3526)
                     /locus_tag="IXF88_001867"
     CDS             complement(1328..3526)
                     /locus_tag="IXF88_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1508593.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            3758..4750
                     /locus_tag="IXF88_001868"
     CDS             3758..4750
                     /locus_tag="IXF88_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1508594.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            4810..6231
                     /locus_tag="IXF88_001869"
     CDS             4810..6231
                     /locus_tag="IXF88_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1508595.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            6224..7723
                     /locus_tag="IXF88_001870"
     CDS             6224..7723
                     /locus_tag="IXF88_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1508596.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            7762..9156
                     /gene="uxaC"
                     /locus_tag="IXF88_001871"
     CDS             7762..9156
                     /gene="uxaC"
                     /locus_tag="IXF88_001871"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1508597.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            9315..9998
                     /locus_tag="IXF88_001872"
     CDS             9315..9998
                     /locus_tag="IXF88_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1508598.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            10000..10836
                     /locus_tag="IXF88_001873"
     CDS             10000..10836
                     /locus_tag="IXF88_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1508599.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPLLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            10847..12601
                     /locus_tag="IXF88_001874"
     CDS             10847..12601
                     /locus_tag="IXF88_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508600.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         3928 a         2429 c         2384 g         3869 t
ORIGIN      
        1 aaaaagaaag tctctatagg agaaagaagt tttacacttt tcaacaatac gtttctaatt
       61 ttgctagctt tgatctgtat cgttcctttt ttaaatatca tcgctacatc ttttgcttct
      121 acacaagaag tcgtcgcgaa aaagtttatc ctttttccta ctaccttttc tttagacgcg
      181 tatcgctaca ttctttctac gccgaccatt tttagagcac ttgctgtttc gattggtgtt
      241 acaggtgtcg gaacgatcgt cagtatgtgt gcaacttcac tgatggccta cggtctttcc
      301 agaaagtatt tgtttggtcg aggtttcgtc aactttcttg ttgtattttc tatgttgttc
      361 agcgggggga tgattccaac gttcttagtt gttcgttctc tagggctggt caattcttac
      421 tgggccatga tcctgcctgt ggctgttaat gcaatgaata tgatcattat gagaaacttt
      481 ttccaagcct tgccggatag tttggaagaa tcagcaaaaa tggatggttg cacagatttt
      541 ggtgtatttt tcaaaattat gctgccatta gcgttaccgt ctattgctac tatctcgctt
      601 ttttatgctg ttacttactg gaacacttat atgactgcta ttttgtatat caacgattca
      661 tctaaatggc cgatacagat tcttttacgc caaatcgtca ttgtatcaag tgggatgcaa
      721 gcagaaagta gtgccgttga tgtgattcca ccagcacaaa cagttaaaat ggctgttatc
      781 gttattgcaa cagtgcctat gctgattgtt tatccttttg ttcagaaata ttttgtcaaa
      841 ggagcactcg taggttctgt caaagggtga ttatgtactg aacgcaaaat ggtagttgtg
      901 tcagaagtgg tcagcttcaa gaaataagaa ggcatttacg aaaattgctt cttaaatttt
      961 tgtgaaattt cggcttattt ttcgaagaat ctactcctgg aacaccgtgt atcagaggtt
     1021 gtgaaagaga cgttcagcct tgagcgatga taaaaactct cacaaacatt gcgacgagta
     1081 acccacagga gcaataagaa gcgattttga aaaatagctt cccatatttc acaaaattat
     1141 gccttattgt cgatagcctg tctcttgaac gccatttatt cgtaaaaaag aactgtgaca
     1201 agacttttgt cacagttctt tttgcgtgct tagccttgaa atttaactat ccataaatac
     1261 aaatactagg gttattctaa cttcttttat tgcttgaatc agtccacaag aaaaacagcc
     1321 tcttgattca caaatatttt tttcgatatt ctcccggcgt aatcgattcc ttctttttaa
     1381 agaaacgaat aaaattttgc ggatttcgat attgcaatct ttctgcaatc tcttttacgg
     1441 agaggttagt ctctttcaac catttttttg ctacctctaa acgatgattt tgaacaaaat
     1501 caccaaattt ttctcctgtt tcctttttaa atacattact taaatagtta gggttataat
     1561 gcaacctatc agcgatcaca tctaaagaca gatcacgatc atattctgtg tagacgatat
     1621 gcataatatt ctccgacaaa tttttaaatt catgatcagt gattgcttga gagcattctg
     1681 tgattggcag aactaattgt tctaaaacaa gtttttctag ttctttggga tgataattgt
     1741 tcagtgcttt tacataaatt tgtttcatgt tatcaaagtt ttttgaatct actcccaata
     1801 actgtcctaa ttgaacgagt tcattgatca agcggaccga aactacttcc cgactcaaag
     1861 gatttttatt ttgtgcaaac aatgcatcaa tcaattcatg tactaaattc ttcacttcct
     1921 gatttccaga acgaatggct tcaaacaatc gattttgttc ttctatcgga tatctaataa
     1981 gtgttttttc atgagcagca ggaacgattt cctgataaaa aataattgaa cttggaccag
     2041 tatttacacg ataatacagg gcttcttttg cccgatcaac cgattgcttg ctttcattca
     2101 gcgtttcgaa ccggttactg atgcctatac tgatagttaa attcaagtat tcttttacac
     2161 gtttttgaat gagtcggcaa tactccatga cttttttgct tgcttctgca tcatctttat
     2221 ccattcgata aatagtggct tgcatttctt cattcaaaac gatcggcagc attcgatttt
     2281 cctttggcac aatttccgtg atcatgttgt tgatcgctaa aagtagcaaa tcacgctccc
     2341 caccgtgact atcttctaac agatcgattt gaaccaaagc tgtataatag cagtcatttt
     2401 gagaactata accaaattgc tgcaaccgct gatctagttc tcgcctttct actcgatttc
     2461 taaataaact aagtacaaat agcgtttcca gctgtggctt ttgggtaact aagtgtgcac
     2521 ttaacgcttc tttttctcct atgatccgac tgactgattg agcgaccaaa tctagttctt
     2581 tcccactgaa tccttttgat ttaagattca gacgttcctg tatttgagaa atcggacggg
     2641 caaatctgtc cgaaaataag taggaaagaa gaccaatgag tcctaccagt gttaaagaca
     2701 ctgcaactag cccgattctc agattgcgga tcattgcgcc aattgcacta gagtcaactt
     2761 ctaatctgta aatccaatta ttataatctg atttcagtac tatatacgtt ttatcttttt
     2821 ctcttatcac tttcagctga ttgtaatcta gatccacgtc cgttaataca gttggttgct
     2881 ctttattttt tactgtctga gtagtaaaaa ggttgccaac ttcgttttca atagaaagta
     2941 tgtcttctcc actttcacca acaacttgtt ctattgtatg tttctctatc gtagcaattc
     3001 ccaatgcata tttttctttt tcaaacattg gcaaagaaat caccatttcg attcctttgt
     3061 ttttcttttg ccaaaataaa ttatttttgt tatttatgta cttctgttga tacttttcaa
     3121 cttcctcttc ggtcagttga ctcaacgatc cattgattac tccccattta ccagccaaac
     3181 tgactaaatc ataattatta tcatccataa caatcgtttc tataaaattt agttctttcc
     3241 tgatttcaga ataagtggag taatctgtat atgtcagcgg atgcttgaat atgtccgaga
     3301 aatttttggt cgaaccatac gtagaaaacg aatattcaat cgttctaatt ttccattcaa
     3361 cgttagtttt aacttgcctg agcaaattct ttttgtctgt taaataactc gattcgatcg
     3421 attcataggt attctggtaa atgaataaac taaatcctat gacaagtacg gctcctaata
     3481 aaaacatcgg taaaaatatt ttataaaata ctgtactttt cttcattttc ttcctctcct
     3541 ccttttaaaa taaaaggctt tcatttttct gtaaaattga tactcatcta attaaatgag
     3601 cattccccgc tttcaagaat agcataatta aaaataaaaa tccaagtaat ttttaacgtg
     3661 tgcaatcttt tcatatcaaa gatctattga cttatcgtag ttatcgtaat atgatgaatg
     3721 cgttaacaaa aagtgaaaaa gtaaggaaaa tgatggaatg gtaacgatca aagatatcgc
     3781 taaagcagct ggcgtttctc atacgacagt ttctcgtgct ttgaataaca gcagtttaat
     3841 aaaaaatgaa acaaaacaaa aaatacgaca actagctaag gaaatgaatt attcgccaaa
     3901 ctatagtgca aaaggtttag tgaatcaaaa aaaatatcta atcggactat ttttctcaag
     3961 tttgcataaa ggaacttcat caaatttcct agcagattcc attgcaggca tccattcagt
     4021 attagacaca aattacagtt tatctgttga aagtatcgat gttgttgatt taacagaaat
     4081 caacctgcaa cgctacgatg gactgatcgt tatgagtcaa agtgatgatg atcagccttt
     4141 catcgactat ctcaaagaaa aagaatttcc tttcgttgtt gtcaatcgtc acatcaatga
     4201 tccagcggtt atcaacgtca ctgccgatga tgcggccggt gttatgcagg cagtagatta
     4261 cggtattgga tgcggacata cgaagatcgc ttatattggc ggaaagaaaa attttcgttc
     4321 gacaaaagaa cgggaaaaag gggtattgaa aagtctaaaa aaagcgagca tccctgtcaa
     4381 tccagcctat tttttaacag gagattacag cttagaaagc ggcctccaaa acatggaata
     4441 cctattatca ctttctgacc tgcctactct ggttttttgc ggcaacgacg acatagcgat
     4501 tggtgcattg agagctgtac atttagctgg cctccgtgtt ccccagaaca tttctctgat
     4561 cgggttcgat gattctccag tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa
     4621 gcctttgaaa gaaatgtgtc aagaaggaac aaagctttta ctagatttga tcgaaggcag
     4681 gacgatcgaa caatctaaaa ttgagctctc caccttgttg atccttcgtg agtcgatcaa
     4741 ttacttgtaa actacaagta attttatata aaaatgttaa cgtgtgcata acacagaaag
     4801 gaagtttata tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa
     4861 gttcttcagt ttggtgaagg caattttatg cgtggattta ttgattggca gattcagcag
     4921 ctgaataacc aacaattgtt taaaggtaat gttgcaatca ttcaaccttt agaacaaggt
     4981 cttggaaata tgatgaacca gcaagataat ttatatacgg tcattttaca aggactcagc
     5041 aatggcgaaa tcgttgatac ttccgagatt atcaccgtag tagaacaaat gatcaatcct
     5101 tatgatgaat gggatgctta tctatctcta gcagaaaatg acgacttggc ctatatcgtc
     5161 tccaatacaa cagaggcagg aatccaattc gtggagactg ataatttaac agatactccc
     5221 ccacagagct tcccgggcaa actgacagcc tttttatacc gacgtttcaa attaaacaaa
     5281 gctggcttta ccatcattcc ttgcgaactg attgacagaa acggcgaacg tctaaaagaa
     5341 atcattttac gctacgcaga aaaatgggat ttagaacctg cttttgttga gtggttagaa
     5401 aaagaaaatg ttttctgttg tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa
     5461 gatgcaggtg ttttcaatca acggcataac tatgaagatc aattgatggt caaagcagaa
     5521 ccattcatgc tttgggtgat cgaagggcct aaagaattgg agaatcaact accattcaaa
     5581 caggctggct tgaatgtcat catcacagat gatatgactc cttatcgtga acgtaaggtg
     5641 catttgctta atggaccgca tacagcgatg gtccctcttg ctttgttggc ggaattggaa
     5701 actgtagaag aagtaatgaa agatccttta ttctccgcat atgttgatca gttattcaat
     5761 ctagaattga ttcctatgct ttctttacct aaagatgaat tagccattta tgctgaccaa
     5821 atcaaagaac gtttcttgaa tccttttgcc catcacaaat tagaagcgat ttcattaaat
     5881 agtgtttcaa agttcagcac ccgtctgctt ccagtgttca agaagtatat cgaagaacaa
     5941 aatcaggtac caccgttaat cactgtttca ctagctgcgc tacttcttat gtatcgaggc
     6001 gatcaagtaa agccacatga tgatgaaaag acgataagcg aatttactga tgcttggtct
     6061 gataatggaa cagctattcc tcgattattg caaaacgctg cgctttgggg agaagatttg
     6121 tctcaaatac cgaatgtgac agatacagtt caagaaatag ctgcaaagat cgatcaaaaa
     6181 ggtgtccggt cagtattaca agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca
     6241 accaacaatg aaattaaatc gacgagacag tgtggtcgtc gcattaaccc ctattccaaa
     6301 acaaacacaa ctgacgattg atcagcaaac gatcaccact cttgaagata tcccgcaagg
     6361 tcataagatt gcattgggtg atttgaaagc tggagacaac gtgatcaaat atggctatcc
     6421 aatcggtcat gtgacgacag acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa
     6481 aaccaatctt tctggtgaat tggattatcg atatgaaaaa gatgtccatc caagtcatta
     6541 cctttttgag aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg
     6601 taatgatttg tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt
     6661 caaacagttc aaggaaaaac atccagatct gggacagttt gaccatatta cgattttaaa
     6721 gcatccttat ggctgttcgc agcttggaaa agatcatcaa aatactcgag aaattttagc
     6781 ggacgcagta aatcatccca atgccggagg agttttagta tttggattag gttgtgagaa
     6841 caatacagtt cctgaattca aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt
     6901 tctagtggct caagatgttt acgatgagat cgaacaaggt gtggctctat tagaagagct
     6961 tttaacagcg gcggaaaatg accagcgcat atcagtcccg ctctctaagt tgaatgtcgg
     7021 tttgaaatgc ggcggttctg atggtctatc gggaataact gctaatcctt tacttggtgc
     7081 attttctgac tacttgattg ctcaaggagg cagcacgatc ttaacagaag tgccagaaat
     7141 gtttggcgca gaacaagtat tgatggcacg agcggaaaat gaagaagttt ttgactcaat
     7201 cgttgacttg atcaatgact tcaaacagta tttcttgtct tacggggaac cagtttacga
     7261 aaacccatct cctggtaata aagcaggtgg gatcactaca ttagaagata agtctttagg
     7321 ctgcacgcaa aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat
     7381 cagaaaacca gggcttagtt tattgcaagc tcccgggaat gatcttgtag ctgcctctgc
     7441 tcttgcttca tccgattgcc agttggtctt gtttaccaca ggaagaggga ctccgtttgg
     7501 ctcatatgtc ccaacggtca aggtatctac gaatacaact ctttttgaac gcaaaggtca
     7561 ttggatggat tttaatgcag gcgtcttatt agaacaacca atggaagtaa tcttagaatc
     7621 gtttgttcaa aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg aacaaaatga
     7681 cgttcgagaa attgccattt ttaaaaacgg agtaacactt tgattttcat aaaacagtat
     7741 aaaaagaaag aaggaagatt tatgttttta tctgatgatt ttttattaaa agacgattgg
     7801 gcgaaaaagc tttaccattc ctacgcaaaa aacatgccca ttattgatta tcactgtcac
     7861 ttagatccaa aagaaattta cgaaaacaac aatttcagca atctgacaga agcttggtta
     7921 ggaggcgatc attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc
     7981 acaggaaatg cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt
     8041 atcggaaatc cgctttacag ttggtcacat cttgaattga agcgtttctt cggtattgac
     8101 ttgttgataa atgaagaaaa tgcagagaaa atttggacac aagcaaatga aattttggca
     8161 gctcctgatt ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca
     8221 acagatgatc ccgcagacga cttgcactat catcagttgc ttgctcatga agaaccagat
     8281 ttgaaggttc taccttcatt ccgtccagat aaagcactca atattgaaaa agaaggattt
     8341 aatgcatggg tcacactact ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa
     8401 ctgatagaag tcttagggca gcggatagaa tacttccatc aacatggatg tcgtgtttcc
     8461 gaccacgctt tagacatctt acgctataaa gaagcggatg aaacagtatt agaagagatt
     8521 tttcaaaaag cacgagctaa caaactatta tcttcagatg aaatcgatgc ttatcgtaca
     8581 gaaacactga tacatttgac ccacttctat cattctcata attggacgat gcagctgcac
     8641 atccatgctt atcgtaactg taatacacaa atgttggaaa aattagggcc cgataccggc
     8701 tatgatggaa tgaatgatct ttctttgact attcctctgc aaaaattatt gaaccgtgca
     8761 gaagaaacgg atcagttgcc aaagacgatc ctttattcat tgaatccgaa tgactatcct
     8821 gcgattttag ctttgatggg ctgtttccaa aaagaacaga atggaaaact gcagttaggt
     8881 tctggatggt ggtacaacga tactcgagct ggtatgcgcg atcaattgac acaatttgct
     8941 gatggtagtg ctttggggaa ttttgtcggc atgctgacag attcaagaag tttcctgtcc
     9001 tacactagac atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg
     9061 cgaggagaag cacctgaaga tgaacaactt ttaggaagtc tagttcaagc gattagttat
     9121 acaaatgcta aaaattactt tggctttttt gcctaattga ttaatagtaa cagtaacgaa
     9181 tcaaaaacag gatagtgaaa taacggaatc cgtttatcca cgttttagag aagaagacgg
     9241 accaaatttg ctccgtcttc ttctcttgct gttctgcttc attgcctata aacctttatg
     9301 aaagaagaga taacatgcct actatccata ttgccggtga ttctactgcg gcagaaaaag
     9361 atgaaaacaa gcgtcccgag acaggttggg gagaaaaaat ctctgcttat ttttcacccg
     9421 agatccagat caataaccaa gcaaagaatg gacgcagcac caaatcattt atccaggaag
     9481 gaaggttagc ccgtctagct tcacagtttc agccgaatga tttcttattt gtacaatttg
     9541 gacataacga ccagaaaatc caagaagaaa gaggtacgct tcccttcggt agctatttaa
     9601 aaaatctgca gatatttttt gaatgcgcaa aaaaagcgaa cgtccaaatg gtcttactga
     9661 catctgtgac gcgacgagat tacttagaaa acggaacatt gaatccagat atacttggag
     9721 actatccaaa agcgatgcgt gcatttgctg agaaacacca tattcctctt ttagatgtgt
     9781 tctcaagatc acaagaattg ttccagacct tctcaaaaga agagacaaaa aaattctacc
     9841 tgcatcttat acctgatact tgtaaaaatt acccagaagg actaaaagac aatacccact
     9901 ttagtccaga aggcgctgat aaagttgccc gaataattgt tgaattgata aaagaatccc
     9961 gtctgcctct tgctaattat ttacaaaaag gagtttgaga tgtttccatt aaatttaggc
    10021 atccgtgccc acgatttaga tgttcgaaat cgtgaagaaa tcgttcaaaa agtatcacag
    10081 cttcacttgt cccatatcca atttgcacca caaaaagcat ttcctgaaca acttaaatta
    10141 actgacatga cacttgggag tgctgcctat tttggtgaat acttccaaaa aaacaatatc
    10201 gaactttcca ttttaggatg ctatatcaac ctatccagca aaaatcaaga agttcgtgca
    10261 caagcattgg caactttcaa acagcaaatt gctctgtgct ctgcctatca agccaagatg
    10321 attgctacgg aaaccggtag tgtctctgtc ggttacacaa aagaaaattt tactgaagaa
    10381 gcttatcaga tcgccagaaa ttctatcatt gaattagtcg ctcatgcaga aaattttggc
    10441 gtaactgttg ctgttgaagc agggatcaat catccaatct ataactatca gttagctaaa
    10501 cgtttaatcg atgaagtagc ttcaccaaat ttaaaaatca ttttagattg tgcgaatttg
    10561 atgcataaag aaaatcatgc tgatcaggaa aaagtgatcc gaggtgcctt ggacaattta
    10621 catggttata ttacagcgct tcatctcaaa gactatatca tggaaaatgg aaagatccgc
    10681 atggtacctg ttggcaaggg ctgtttagat ttccgcccgt tactgcatta tatcaaatat
    10741 gagcgtcctc taatgtacgc cactcttgaa gctactcctg agatttatgt tcctgaagca
    10801 atagaacact tgcagtttat ttaccaagat tgttaaggag aagattatgg aggaacgaat
    10861 acagaaaagt tatcaaaaat atcatgcaaa tgcttttcag acattgctgc atctttatca
    10921 gggaaattat tggcgttttg ccatgtcagg actctttttt attatcaaac atcttccatc
    10981 ttggttaatg ccgatcgcta ttgccaatgt aatcaatgca gcttcatcga aagattcttc
    11041 tcagttgcag gccatttatt tcaacgcttt tttgatggct tgtctgattg gtcaaaatat
    11101 attgacgaat tattttcatg taaaatacca tagcacttcg atccgtcaag tagaagctgg
    11161 actaagagcg acccttatca agaaaatcca agaactatct attccttatc aaaaagaatt
    11221 gcaatctgga cgaatccagt caaagatcat ccgtgatgta gaagctatcc aaacgttgtc
    11281 ctctcaagta tttgtcagcg gattaaatat cgctgtcaat ttattcgtag ctttaggtat
    11341 cacgctttat aaaagtcctg ttgttttcat gtttttctta ttgactgttc ctctcgccgc
    11401 attcgttact ctctttttcc gtaaaaggat cagacagtcc aactcagact tcaggcaaga
    11461 aatggaagag acttctgcaa aagtgatcga agtgatcgaa ttagcaccga ttgctcgagc
    11521 tcatgggtta gaaaaacacg aaacaactcg cttaaataag cattttactc aaatctatga
    11581 aaaaggattc cacttagata tcctgcaatc tgttttcgga tcagtcagct gggctagttt
    11641 tcagatattt cagctgattt gtttgatttt cactgggata ttagctttac aagggcgcat
    11701 ccaacccggc gatgtcgtga tgtatcagac gtatttcacg acgatcgtca attccgtttc
    11761 tggaatgatc acgctgattc ctacgatttc aaaaggcatg gaatctatca cttctattgg
    11821 cgaactgttg attgctggcg atgtagaaac ttatcacggt aagaaaaaaa tccaacattt
    11881 ggatggaaat tttcgttttg aggatgtttc ttttctttat ccggactcac tcaaaccgat
    11941 tatcgatcat ttttctctag aggtgaaagc tggagaaaca attgcttttg ttgggccatc
    12001 tggctctgga aaatctacac tccttaactt attgattgga tttatccagc cgacttcagg
    12061 aaaaattttt ctagatgatc agcctttgga tgaactggat atgcgaagtg tgcgaaatta
    12121 tttagccgtt gttcctcaaa ccactttgtt gttctccgct tcgatcaaag agaacatcac
    12181 ttatggcttg aaaaacgtat caaaagaacg tttagatgaa gtcatcgaag ctgcgcaact
    12241 ttcttcactg attgaccagc ttcctgatgg tctggatacc ttagtaggcg aacatggcaa
    12301 caagctgtct ggtggccaaa agcaacgtat ctccattgca cgagcgttga tacgtcagcc
    12361 aaaaatcatt ttgctagatg aagcaacctc agctttagat aaccagtcag agaaaaaaat
    12421 ccagcaagcc ctgaattacc tgacagaaac acccactact tttattgtgg ctcatcggct
    12481 ttcaacgatc aaagaggcag ataaaatcgt tgtgatcgat gaaggaaaaa tcgttgaaat
    12541 aggtacttac gaaaaactgt tagctcaaaa aggctatttc taccgcttga aaaatgaatg
    12601 acaaaaaata
//
LOCUS       DACESX010000082        11837 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.082, whole genome shotgun sequence.
ACCESSION   DACESX010000082 DACESX010000000
VERSION     DACESX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.082"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..177
                     /locus_tag="IXF88_001875"
     CDS             <1..177
                     /locus_tag="IXF88_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508601.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
     gene            578..1183
                     /locus_tag="IXF88_001876"
     CDS             578..1183
                     /locus_tag="IXF88_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ1508602.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="IXF88_001877"
     CDS             1199..1771
                     /locus_tag="IXF88_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1508603.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="IXF88_001878"
     CDS             complement(2023..2280)
                     /locus_tag="IXF88_001878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508604.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="IXF88_001879"
     CDS             complement(2406..3221)
                     /locus_tag="IXF88_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508605.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="IXF88_001880"
     CDS             3738..5063
                     /locus_tag="IXF88_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1508606.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="IXF88_001881"
     CDS             5056..5406
                     /locus_tag="IXF88_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508607.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5388..5573
                     /locus_tag="IXF88_001882"
     CDS             5388..5573
                     /locus_tag="IXF88_001882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508608.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6477..6752)
                     /locus_tag="IXF88_001883"
     CDS             complement(6477..6752)
                     /locus_tag="IXF88_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ1508609.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6724..7677)
                     /locus_tag="IXF88_001884"
     CDS             complement(6724..7677)
                     /locus_tag="IXF88_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1508610.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8289..9782
                     /locus_tag="IXF88_001885"
     CDS             8289..9782
                     /locus_tag="IXF88_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ1508611.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9917..10213
                     /locus_tag="IXF88_001886"
     CDS             9917..10213
                     /locus_tag="IXF88_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1508612.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10314..11165
                     /locus_tag="IXF88_001887"
     CDS             10314..11165
                     /locus_tag="IXF88_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508613.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11186..11398
                     /locus_tag="IXF88_001888"
     CDS             11186..11398
                     /locus_tag="IXF88_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508614.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4075 a         1820 c         2197 g         3745 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 ttactatact cactatatta aatatattta gtatagtaaa aactagaaac aataaaattt
     6361 gtgaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
     6421 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
     6481 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
     6541 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     6601 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     6661 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     6721 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     6781 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     6841 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     6901 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     6961 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     7021 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     7081 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     7141 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     7201 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     7261 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     7321 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     7381 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     7441 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     7501 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     7561 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     7621 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     7681 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     7741 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     7801 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     7861 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     7921 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     7981 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     8041 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     8101 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     8161 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     8221 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     8281 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     8341 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     8401 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     8461 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     8521 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     8581 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     8641 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     8701 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     8761 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     8821 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     8881 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     8941 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     9001 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     9061 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     9121 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     9181 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     9241 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     9301 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     9361 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     9421 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     9481 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     9541 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     9601 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     9661 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     9721 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     9781 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     9841 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     9901 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     9961 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
    10021 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
    10081 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
    10141 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
    10201 gtatatccag tagtagtttt tatttactta aaaaattggt tcattgagca acctatcgct
    10261 gctaaagtag caaaggaaaa cgttaaagga aagaatttag ggaagaaatc gaaatgaaat
    10321 cggacgactg gattgaacac acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat
    10381 tttgggtatt gcaattatgg ccattaggaa aactttgtag ttacggggca cgacttattc
    10441 ttgatttaac cgtttactcg attggagtat ttggaacggc ttattacttg ataaatgatc
    10501 ccaataggat tgcagcatca gacattttta cagtgctaat gacgttgatt tttgtcatgc
    10561 tattgtctat ttttactcaa ttagtcatgc aatttaccag gcaaaaatta acagcaggaa
    10621 aacttttcaa aagcctattc gatttaattg ttgtaaatgc aattctagcg actttaataa
    10681 aacaaattga acaatctgga agaacaacaa aaggagtgaa ggatttttta ttgacgttta
    10741 ttcactttgt aaatggacac atgtatttgt ttgaagtggg tattattctc ttttttgtcg
    10801 ggtatgtatc cacgaaactt attaaacgtt caattgaaaa agaaattgaa attgacctga
    10861 ttccgacact tcttggttat gagtacaatg ttgattttgg aagatatgac gaattgatca
    10921 atacggatat cgttcacacg agtatccatg aatctactgt taagggcatc agatctattt
    10981 taaaaaatgg ccaggaaatg aaagcaatag atcgaagaaa agagatctac aatttttatg
    11041 aagtcgaagc aagcctacga tatggcatcg gaaaattgtg gttagatgga agtaatattc
    11101 gtcaaaacaa accatacatt ttttggaaca gtagtgattt agaacttcca atgaaagata
    11161 aataattatg gagaatcgtt tgaaaatgat tcctctatac aggattgtgc ttatagttgg
    11221 catggagtta aaaaccttaa agaaggagca gttattcaaa gtgaaagaac gtcataatca
    11281 gttactatgt agtaaaacgg gtacgatgaa cattgaatac cgaacagaat atttagaatt
    11341 aacgaatcaa actgattttt tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg
    11401 gctacttata gccaattgat tgatgaatgg gaacaattca aggaatctaa aattccagaa
    11461 cgtctctaaa agcctttatt tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa
    11521 aagtgttcca ataggctgtg cctattacgt ttctcaaaaa taaatgtggg aacaaaatat
    11581 ttaaaaatag gatagtgaaa ggatagtgaa ggatagtaaa aggagggtag taggatagca
    11641 aaaaggatag gctccttttc gctatccttt ttaaattgga ggaattaata tggaagtaag
    11701 aataagaaat gtggatgaga aaatcgtaag agaaattgat cgaagatgtg acgaacggtt
    11761 ctgttgcaaa gttttaaatc tactatcaaa taaggtagaa taatagaaaa agatagcagg
    11821 aggaatgacg atgaatc
//
LOCUS       DACESX010000083        11633 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.083, whole genome shotgun sequence.
ACCESSION   DACESX010000083 DACESX010000000
VERSION     DACESX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.083"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(182..2257)
                     /locus_tag="IXF88_001889"
     CDS             complement(182..2257)
                     /locus_tag="IXF88_001889"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1508615.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            complement(2259..3176)
                     /gene="glyQ"
                     /locus_tag="IXF88_001890"
     CDS             complement(2259..3176)
                     /gene="glyQ"
                     /locus_tag="IXF88_001890"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1508616.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3560..4372)
                     /gene="recO"
                     /locus_tag="IXF88_001891"
     CDS             complement(3560..4372)
                     /gene="recO"
                     /locus_tag="IXF88_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1508617.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4515..5414)
                     /locus_tag="IXF88_001892"
     CDS             complement(4515..5414)
                     /locus_tag="IXF88_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1508618.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5429..5830)
                     /locus_tag="IXF88_001893"
     CDS             complement(5429..5830)
                     /locus_tag="IXF88_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1508619.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5808..6284)
                     /gene="ybeY"
                     /locus_tag="IXF88_001894"
     CDS             complement(5808..6284)
                     /gene="ybeY"
                     /locus_tag="IXF88_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1508620.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6301..8493)
                     /locus_tag="IXF88_001895"
     CDS             complement(6301..8493)
                     /locus_tag="IXF88_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1508621.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(8517..9488)
                     /locus_tag="IXF88_001896"
     CDS             complement(8517..9488)
                     /locus_tag="IXF88_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1508622.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10194..11573
                     /gene="brnQ"
                     /locus_tag="IXF88_001897"
     CDS             10194..11573
                     /gene="brnQ"
                     /locus_tag="IXF88_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1508623.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3164 a         2430 c         2127 g         3912 t
ORIGIN      
        1 ttttcttgac tagggaaatt cctaatttaa aagtaaataa aacagtaaca agattattat
       61 taatcttttt catttttatt tcctcacttt tcatgagcgt tttgaaaatt ctagcaaaga
      121 ataaaataac aaaaattatt aaaatagaat taataacagc ttttatattt ataacaaaag
      181 actattttgt gatgatttga tccaagcttg ctaatgacaa agccatggca gttatttgac
      241 taagttgtgt tagtcggttc ttacgtactg ctggttcatc agccatgacc atcgtttgat
      301 caaaataatt ttcgatcagt ggacgaagag cgacaagctg gtcgtagttt tcgtggatcg
      361 tttggtcagc aaaacttgct ttcattgctt ctactgcttt gtgtaattct ttctctgcgt
      421 cgttttcgaa cagctcaggg tctacttctg attgtttttc aagctgcgct tttttcgcta
      481 agttcacgac acgtgtcaat gcttccattg aaggtttgaa gtcttcatct gcaagatgtt
      541 gtttgaacaa ttgtgctgct gcaaatactt ttgtcagatc tttttgttct gcattcaaaa
      601 cagcttcgat gacatcatga cggatattct tcgttgtcaa taattggcgc agacggcctt
      661 tgataaagtc gatcacctgt tgttgtcccg ctgacaagcc aatgccaaaa cgatccgtat
      721 ctttactgat aacttcacta atcgtttctt gcagaacaga caaaggaaag gcccattttt
      781 tcgattcgat gattcgaacg acaccatatg cttgacggcg caaagcatat ggatcgtttg
      841 agccacttgg gatcatccca actgcaaaga aagaaaagat actatctaat ttatcagcaa
      901 tcgctaaaac agaacctact gtagtctcag gtaaatctcc ttcactggat ataggcatgt
      961 aatgttcacg gatcgctgtt gctacggctg gtttctcgcc tttaagcaga gcatattttt
     1021 ctcccatgat cccttgtagt tcagggaatt ctcctaccat gttggttact agatcaaatt
     1081 tatagatttc agcagcgcgt tgcagatcca tcaattcttc ctcagatagt cctacttgtt
     1141 cgcctatgat gcgagcgatg acttgtaccc gttgcatttt ctcatagatg ctaccgattt
     1201 tttcatggaa agtaacagtt tttaattttt caacgtattg gtcgattgaa gctttcttgt
     1261 cttcattata aaagaattcc gcatcttcta aacgagcagt caatactttt tgattgcctc
     1321 gtaccacatt ttccaatttt acccgatcgc cattacgagc agaaataaaa tgaggaagca
     1381 acataccttg atcgttccgt acttcgaaat aacgttgatg ttctttcata gaagtgacta
     1441 gtacttcttc aggaacagat agatattttt cttcgaagtc gccagaaaag acagttggat
     1501 attcgactag attgttgact tcttctagta aatcttcatc tagatcaatg atccagtgat
     1561 ttttttctgc taattcgttt gcttgtgtga tgatcatttc ttttcttttt tgcggtgaag
     1621 caataacaaa ttgttcattc aatttttctt cgtattcttt tgcatgcgta aatgtcacat
     1681 cttcgcctaa gaaacgatga cctcttgaaa ctctgtctgt tttgacatcc aagatctcaa
     1741 acgggattat ttcgtcatct aacaatgcca cgatccagtg aatagggcga atatattcaa
     1801 aatcgtattc tgcccagtgc atcgttactg ggaaagtgag gcttttaatg acatctgcta
     1861 aacctgtcaa tacttctttt gcttctttgc cgtgagtgta tttcgttaca tagacatatt
     1921 ccacgccttt taattctcta aaagtgatag catctgtcgt cagaccttgt ccgcggacaa
     1981 atccttcagc tgctttcgtc cagtttccat ctgcgtccaa agcaattttt ttggctggtc
     2041 ctttgacttc ttcttcgctg tctgcttgac gttctggaat gtttgttact ttgattgcta
     2101 agcggcgcgg tgtagaaaac gtttcgatcg tatcataagt caattggttt tcatctaaaa
     2161 attttgctgt tttttcttct aattgtttca tgcttggtgt gactacatga gcaggcattt
     2221 cttccagccc gatttcaagt aataagtctt ttgccatctt attcagcctc cttcacgttt
     2281 tctggatttg ctgtgttttt attcaataaa gggaatccta atttttcgcg ttctgccaca
     2341 aagattttgg ctacagcacg agccatgttg cggatacggg aaagataacc tgcacgctct
     2401 gttacggata ctgcaccgcg agcatctaat aaattgaatg tatggctaca tttcaaaata
     2461 taatcatacg ctggatgaac aagattttca tcgatacagc gtttagcttc tttttcaaaa
     2521 cgatcaaaat tgcttagtag caggtcttgg tcgcttactt caaaagaata tttagaatgt
     2581 tcatattctg gttgtttgaa gatctcacca tatttcacgc catcagccca ttccagatca
     2641 taaacacttt caacttcttg gatataagaa gcaagacgtt ctagtccata agtgatctca
     2701 gaagtaactg gtttacaagg tagtcctccg acttgctgga agtacgtgaa ttgtgtgatc
     2761 tccatcccat caagccatac ttcccagccc acaccggcac aacccatcga tgggttctcc
     2821 cagttgtctt caacaaagcg aatatcgtgt tctaatggat caattcctaa aagacgcaag
     2881 ctttctaaat aaagctcttg gatattttca ggtgaaggtt tcattacgac ttgaaattga
     2941 tgatgctgat acaaacggtt tgggttttct ccataacgcc cgtcagctgg tcggcgggat
     3001 ggctctacat aagcagcatt ccatggttcc ggtccgattg cccgtaagaa tgtatatggg
     3061 ctcatcgtac ctgctccttt ttctgtatca taagcttgca tcagcatgca gccgtttgat
     3121 gaccaaaatt tttgtaatgt taaaatcata tcttggacgg tcaatttttt actcatctgt
     3181 tttgcccctt tcaattttta agcgttgctg tcaataagaa tacaggaaag aaaacgacaa
     3241 aaagtcccta tgtctgatta cagacatagg gacgaataat ttcgcggttc caccctactt
     3301 ccgaataaag attcggcagc ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt
     3361 tgggtccggc ttccactctc ccgaactcgc taaaccacgt gttttttact gatcttggtc
     3421 aatgtacgta ttcattttgc agttttctgg tttgtataat agatcctctc agtgaacgat
     3481 ctatttaatc tacggttaat aatagacgga agtccctgta tttgtcaaat agttttgatt
     3541 tttttgttgt atctttcatt tattcttctt tttcaggtat ctctttttca ggcatatttt
     3601 tctctggcgt ctttaatacg tgtgcccaag acttcatttg atcaatgaac tttttgcttt
     3661 ttagatgaat accgacatat tcatcataca actggtcaat gaggtcacgg attgcctgct
     3721 tcgtttcttc tttcaagtgg attccactga tcttatcata agaaatagcc gaaaacattc
     3781 gaacgaaata aacagcgcga ggatctgcat gataacggtg ttcgtccatt ggccagtgtt
     3841 tctcgcatat cacaccgcca tatttggaag aatagtcaaa ttttcctttt gtttccccgc
     3901 atacgacaca gtgcgtccaa attggcgcga ttccgaagcg ttgcagtatt tgcacttcaa
     3961 agatattcgt gatgatttct gcatcatctc cttggtccat ccgctgcaga gcttgttgcg
     4021 taaattggaa taaatgcggg tcatattgat gatcttcgat tgctgcatct actagattta
     4081 gcaaatacgt accataaccg ctcaaaaaga tatcttcttg gatgttccga aacgcatgga
     4141 cgtctttgac actgcttaaa aacgacaaac cttcacttcg aaaattaccg atatagactg
     4201 cttcggtaaa ggggagcaaa gccggagcta atggctgatt ttgccgatgg attcctttga
     4261 tataaaacat cattttgcca gaagattccg taaagatttt gactaattta tctttttctt
     4321 tgtaattacg gctagataaa atcaagcctt tcgattctac aagctggctc atattctccc
     4381 tcctaaaaaa taaatctgga aaagcagatt ctgccgatcc agattattta tgtttcgatc
     4441 ttccgctgtt tttctcaacg tacggatttt tatccagttt atttctatac ctaacctata
     4501 agaagtccag aatatcagta ctcgtctttt cggtagccga agtcttgcag atagactttt
     4561 ttgtctcgcc agtctttttg gactttgacc caaagttcta gaaatacttt gtctcctaaa
     4621 agattttcga tatcctttct tgctttcgtt cctacttgct tcagcatttt tccgcctttg
     4681 ccgataatga ttcctttttg gctatctctt tcgacgatga tcgtggcctg gatatggatt
     4741 ttatcattct cgtttcgctt catcgaatca acaacgactg cgacagaatg aggcacttcg
     4801 tctcgtgtta aaaacaagac tttctcacgg atcaattcag aaacgatgaa gtattcagga
     4861 tgatcagtca cttgatcatc tggaaaatat tgtgggccct ctggcatttc actgactaat
     4921 acatccatta agcgttcgac attgtttcct tctgtagcag agatcggaat cacttgtgca
     4981 aaaggcatct gcgtcgaata atcttcgatg attcccagca actcgtctgg atggatctta
     5041 tcgattttat tgataatcag ataaacagga gaatgcacgt tttttagacg ttcaataata
     5101 aaatcgtctc cccggccacg tttttgatca gcactgatca taaataagac tgcatccact
     5161 tctttcaacg cactgtaagc tgcttctacc ataaaatctc ctagacgatg ctttggttta
     5221 tggattcctg gtgtatcgat aaaaatcagc tgagcttctg gtgtagtata tactccttgg
     5281 attttatttc tagttgtctg tgccttgtca ctcataatgg caattttttg acccacgatt
     5341 cggttcaata atgtggattt tcctacattc gggcggccga cgattgcgac aaaccctgat
     5401 ttatgttctg gcatatacgt tctccttttt aattaaacaa caaatgatat attttaggga
     5461 caaataaaat caacccaatg atgacagcaa aacaagcggt cactaaaact gctccagctg
     5521 ccatatcttt gatttttttc ccaatcggat gaaaatgaaa atccgtaaac atgtccacta
     5581 cattttcaaa aacagtattg atgatctcta ctatccaaac gagaaaaaca gctaaaagca
     5641 gccataacca ctctatccga tcaagctgga aaatcacgcc cataaataaa gcaaggatac
     5701 cgaacgcaac atgtttcctc atattgcgtt cttcctcaaa aacggttttg actccttgga
     5761 cagcaaattc cacagacatc aggaaatgtt tgtttttctc tacgttttta tcttttgagt
     5821 ccataggcat ccaagatctc tttctgtaaa ccaaacattt ctttctcatc ttctggtgtc
     5881 atatggtcat acccgttgat atgcaaaaat ccatgtaccg ctaaaaagcc aagttcccga
     5941 tcatatgaat ggccgtaagc ttctgcttgt tcagcagcac gttcaataga gatcatgatg
     6001 tctcctagta cacgaggtaa tggattctct ccatcttcaa aaatcactgg gatttcgtct
     6061 tcgccttctt cttccagagc aaaactgatg acgtctgtcg gtgcatcttt tccacggtaa
     6121 tcacgattga ttacttgaat tgccgcattg tccatgaaag taacagacat ttctgtttcc
     6181 tctggcagct ttaaataaga tgctgcaaat tgcagcaagt catcgatttc tttcattttc
     6241 tcttctgaga cttggtttgt ttcatccata aaggtaatat ccattataca tcgcttcttt
     6301 ctattcttct tgtacttcct ttatcttctc aacttctgtt ttcattttag gatatttgat
     6361 ccgagaatgg aaggtgctac ttaaaccgct gattaatgat ttttccactt ttcggatttc
     6421 tttcaaggtc agtccactat cgtctaattg accatcggaa atacgctctt cgataagatt
     6481 atggacaaaa ctttcgattt tttcgctcgt cggatgatcc attgcccgta ctgcagcctc
     6541 acaactgtca gcaatattga cgatccctgc ttcacgactt tgcggttttg gtccaggata
     6601 acggaattgc tcttctgtta cttccggatt gcgttctttc gctttgatgt agaaataacg
     6661 cattagcgtg gttccatgat gctgtttgca aatatcgatc accatttgcg gcatattata
     6721 atcttccaat attttggctc catctgtcac atgtccaaag ataatatgtt tactgtcttc
     6781 tggcaataag aaattgtgcg ggttttccgc tcctgaaggc aagttttcaa cgaaaaagct
     6841 agcatgtttg atcttgccga tatcatgata atagcaagct actcgagtta gcaaagaacg
     6901 tccgccgatt tctgctacag cgttagcact tagattagct accatcatac tatgatgata
     6961 agtacctggc gcttcttcca gcaactgttt caataaagga tgatttggat tgctgagttc
     7021 atttaataca attacactgt catcattgac catcaattca atatatgggt gtagaccgat
     7081 cgtcagcaag aatgagaaga gtgcaccagt gaacccgcag acgagcatta accaagtcgt
     7141 tgcatcacca aagctcattc cttgatagac gatcaaaatg acatccatca tcacaggaaa
     7201 aataatgacc cacatggcag cagataatcc ttgttctgag atccgtttgc gtctgacaat
     7261 cgttgccatg agcccagaga aaacataaga catcaagata atcgttagaa agttcgtgcc
     7321 aatcgaatca taaaaaatga aaagtgcgga tactgcttgg aaaataccag ccatgatccc
     7381 tgctcttcga ttcaagaaaa agcttaaaac aagtggtgca aaagcagcgg gataaaacag
     7441 cgggatataa gtagcttgtt ccgtttggaa taattggaaa aatttcatca gcactatgct
     7501 gactgacata gctgtcacat agaaaagcac gtattttgtt ctttctcctg cattttggaa
     7561 ttgaagtgaa ttataaatca gtacgccgat ttgaagcaaa acagctaaaa ccatggcaat
     7621 tagcggaaaa atcgaagttg tttgattagt cagtcctagt aattccaatt ttttcatagc
     7681 tgaagcatcg atttgattac cttcacgaac aatgatttct ccttggaaga tcatcactgg
     7741 ttgtacagct tctcgtgcgg attgtttcaa ttcttctgtc tttttctcat tcaatacgtc
     7801 attcactacg ataccttgat ccactaagta gcgaatcatt tgttgttgtg ctggtgtgac
     7861 attcaaatat tggatttgct cttctgcttc ttgtttaaaa gcttccaatt cactttcacg
     7921 aacttgtttg ctcatctgtt cgtcaatgag cttcagactc tcatccctca cagtatgaat
     7981 ctcagtttca gtcatttcaa atatcgtcga ataaaaattg ttcggtagtt tttgataaaa
     8041 cacaacattt tctgcattca cattttcaaa atttttcttc agcgcggcga tccgctcttc
     8101 tgtagtaggc tttgggatat tttcatcttc ctttgcctta tcaatctttt cgtcataaga
     8161 tttgttgatg gcatcattcg tcttatcgat caattcaaat agttgcttga tccgattgtt
     8221 ctggtcatct gcaagatctt tttggtaagt gtattctgga gtgactgctt ctgctgccag
     8281 ttttcttttt tgttcggttt cttccgtgtt ctcgatcgtt ttgttcgcac ggatactttc
     8341 ttctgctact tggccttctc tgaaatcatt ggccttttgt ttgacactac taaatgtaac
     8401 gccgcagata atgataaaaa acaagaacag caggaaaggc aatgctgcct ttccaagttt
     8461 ttcttgtagt ttctgcagtg ttttattcaa catgtgctta tcctcctaag caaagcttat
     8521 tcttgatgga gatctgcctt ttcataagct ttgatgattt cggcaactac agggtgccga
     8581 acaacgtcac ttgcttcaaa attaacaaaa tcgattttct tgatttgctt taatgttcgt
     8641 tctgcatgaa caagaccact tgtcgtccct cgaggcaagt ctatctgact tgtatcccca
     8701 ttgacaatca tttttgattg ataaccgaga cgggtcaaga acattttcat ctgagcgact
     8761 gtcgtgtttt gtgcttcgtc caagataacg aatgcatcat ccaatgttcg cccccgcatg
     8821 taagctaaag gtgcgatttc gattacgcct cgttccatca atcggttcgt atgatccaat
     8881 ccaaaaattt ggtataaagc atcataaaca ggccgcagat aaggatccac tttttctttt
     8941 agatccccgg gaagaaagcc tagattttca cctgcttcca cagcaggccg cgtcaaaatg
     9001 attttttgca cttcaccttt tttcaaggca gcgattgcta atactactgc aagaaaagtt
     9061 tttcctgtcc cggctggtcc tacgccaaat acgatatcat gtttggctac cgattcgaca
     9121 tatttctttt ggccgctgtt ttttacccgt ataggttttc cattacggtc tttgacgatc
     9181 tcttcctcat acatctcaac aaaataatct aatgtacctt tttgccccat tttcaatgcc
     9241 gtcacaacat cgggcgtact tacgtggatt cctcgtttga tcaattcctg taatgtccga
     9301 atgactgagg cagccaagga aatctgggat tgctcaccaa tgatctgaat gatttctcca
     9361 cgtgtatgga tggttgtttc cgttgtttcc tcaatcagtt ttatatgctt gtcgtgtgca
     9421 cctaaaagca tgctgacatc atcagcagcc gtcaatctaa tctctaaaga actagactct
     9481 tccgtcaaaa atagacatcc cctttttttc gattaattct ttcctatttt accatactca
     9541 gaagaaacta tatagcaaat aagaaaaggt tctaaaagag ttgcttaacc ataagtacca
     9601 agaaacgtta aaaaactagc cgcttccatt ctctaaaggt ctcgtttgaa taaaaaaagc
     9661 tgatcatgta agctatgcat aagatagagg acaagtattc cgtctaactt agcaagtatt
     9721 ctctgggtat ttttcttgtt tcaaaaaaag aaaacaaaat atttgaaata ttcttgcatt
     9781 tcaaaaaaaa caggtgtatt ataacgtcaa caaataaata tacagcaaag cgtcgaggaa
     9841 aacagtaggc tattcagtga tttattccca gtgagtcatg taagtgagaa atgacaataa
     9901 atcgaataga cgaacatcat ttccgagtca atgtcgaaaa cgtagatcat tgtggctgcc
     9961 tccattatca ggctagcgga taactttatc gaaaagcgtc cgtgaatgaa gaagcttttt
    10021 taagcgaaaa aaggtggtac cgttctagaa tctagagccc ttttcccaag tggaaaaggg
    10081 ctttttttat attttaaggt ggtggttttc atggaatctg atattagtgg tatttctagt
    10141 ttctgcaacg tttattttgt tactcaaaat gataattatt ggaggaaaaa tttatggaag
    10201 aaacaaaaac ttattcatgg aagcaattat tgatggtcag ttcacttatc ttcgggatgt
    10261 tcttcgggtc aggtaatctg atctttcctg tacacttagg tcaaatggct ggatcaaact
    10321 ggatcagcgc tggtgtagga tttgccatct caggcgctct tttcccatta ttagctatct
    10381 tagctgtagt agtgacaaaa agtgatggtc tgtatgactt agctaaacct gtcggaaaat
    10441 ggtatgctgc cttgttcttg atccttgttc atttaacgat cggaccattc tttggtacac
    10501 cgcggacagc agcaactgct tttgaaatgg ctgctaaacc ctttttgcct gaaaaatatg
    10561 attcattagg tatgttttta ttctctgctc ttttcttttt acttgcttat ttccttactg
    10621 tacatccaac acgtttggtc aaatatgtcg gaaagtattt aaactccttg tttcttgcat
    10681 tattagctat catttttgtc gtttctttat tccaacccat ggggaatcta aaccaatctg
    10741 ctgcagattc ttatcagacg aacgctttga caaatggcgt tttagaagga tacaacacgg
    10801 ttgatgcagt agctttactt gctttaagtg tcacttttgt ccatgcagtt cgtgatcttg
    10861 gttttaaaga taaaaaagtc acagaattaa cagcaaaagc tggtagcctg agcgttgcgt
    10921 tagaagtcat gatttatttt ggtctggtcc ttctcggtgc gatgagtttg aataagatgt
    10981 ctttatcaga aaatggcgga actgcacttt ctcagatcac taatttttat ttaggtaact
    11041 tcggtgctag cttcttaggg gtaatggtca cattaggtgt cttcactact gctatgggac
    11101 tcgttgtttc ttttgcacaa gatttccata aactgttccc taaagtcagt tatatgacat
    11161 ggctgcggtt aacgactttt gtctcttttg tcgttgcgaa tgccggattg gataatatca
    11221 tccaatggtc tttaccagta ttgatgcttt tgtatccatt gtcacttgcg ttaatcttac
    11281 tttccttaac agcaaaattc ttccaaaaga gtccatttgt ctatcaagta acgatgttct
    11341 tttctgccgt tccagccgta ttggatatgt tggcaaattc gcctgcatta gtgagtcagc
    11401 aaactgtcgt agcaagctta ctaggatttt atcatcatta tgttcctttt gctgcattag
    11461 gtcttggctg ggtcgtccca acattgcttg gttacgctgg cagcttgctc ttttattatg
    11521 tatatcgcgt atcaggatat aaacaagaag caagtgagtt accagaagaa taaccaaatc
    11581 aaacacaatt taacataaga gatattaaac aggaaaaaaa cagctagaag aaa
//
LOCUS       DACESX010000084        11585 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.084, whole genome shotgun sequence.
ACCESSION   DACESX010000084 DACESX010000000
VERSION     DACESX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.084"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(114..1424)
                     /locus_tag="IXF88_001898"
     CDS             complement(114..1424)
                     /locus_tag="IXF88_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1508624.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(1606..2835)
                     /gene="rpsA"
                     /locus_tag="IXF88_001899"
     CDS             complement(1606..2835)
                     /gene="rpsA"
                     /locus_tag="IXF88_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1508625.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(2970..3650)
                     /locus_tag="IXF88_001900"
     CDS             complement(2970..3650)
                     /locus_tag="IXF88_001900"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1508626.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(3699..4334)
                     /locus_tag="IXF88_001901"
     CDS             complement(3699..4334)
                     /locus_tag="IXF88_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1508627.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4400..5803)
                     /locus_tag="IXF88_001902"
     CDS             complement(4400..5803)
                     /locus_tag="IXF88_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1508628.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(5790..6821)
                     /locus_tag="IXF88_001903"
     CDS             complement(5790..6821)
                     /locus_tag="IXF88_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508629.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            6852..7073
                     /locus_tag="IXF88_001904"
     CDS             6852..7073
                     /locus_tag="IXF88_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1508630.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7238..7975)
                     /locus_tag="IXF88_001905"
     CDS             complement(7238..7975)
                     /locus_tag="IXF88_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1508631.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8128..9468)
                     /locus_tag="IXF88_001906"
     CDS             complement(8128..9468)
                     /locus_tag="IXF88_001906"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1508632.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(9705..11228)
                     /locus_tag="IXF88_001907"
     CDS             complement(9705..11228)
                     /locus_tag="IXF88_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1508633.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         3301 a         2510 c         1932 g         3842 t
ORIGIN      
        1 ccgcttaata gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa
       61 caaacgaaac tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt
      121 ctcctacgag ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga
      181 ttttctaaga aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca
      241 aatgtaggtg gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta
      301 tctgttggtg ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa
      361 gagatacgaa gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac
      421 cgttgtttag tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca
      481 tcacgaatct cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat
      541 ttattcacga cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg
      601 tcttgttccc gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca
      661 atggcacgca ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt
      721 ttacgcatac cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg
      781 gtgtcgattg cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct
      841 aaaatcgcat tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta
      901 atcgtgtcgt cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa
      961 atatcaccaa tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag
     1021 gaataaaatt catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa
     1081 atcactggtt tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg
     1141 ccttcgcgtc cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct
     1201 tgatgcttga tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc
     1261 acgctgaatt ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca
     1321 ggagtatctt cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta
     1381 ccgacgttcg ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac
     1441 aagtttccaa tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca
     1501 attaaacgaa gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg
     1561 agacaaaagc agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc
     1621 atcggttgct ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt
     1681 aaatccagta ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga
     1741 agctggtttt tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat
     1801 ctctaatact ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat
     1861 atgtttatgt gaaatttgag agatatggac tagcccttct actccaggga acacttcaac
     1921 aaaggcaccg aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc
     1981 ttttgtatcg atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg
     2041 ttcttgttca ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac
     2101 atcacttggt tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat
     2161 tccgcctagg tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac
     2221 atctccctca tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac
     2281 taccgcttta tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa
     2341 gctcaatgtt ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga
     2401 tgcagggaca aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt
     2461 aacaggtgct tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt
     2521 tttagcatct aaacggcgtt ttgaaagtag atagctgccg ttttctttat ctttgccgat
     2581 cgtactgata acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc
     2641 taccggcaaa gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat
     2701 tccgacaatc acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac
     2761 ttttacttct tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg
     2821 atcaaattct gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg
     2881 tttttagttt aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa
     2941 aacaattgtt tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca
     3001 acagctttta ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca
     3061 gcatcctctg cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc
     3121 tgtatctctt tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg
     3181 aaacgtcttt ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc
     3241 ggtaatacag ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt
     3301 tttcgttgga cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca
     3361 gcttttgtga catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca
     3421 tgctgtccat cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc
     3481 ccttcttcat ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac
     3541 atggcaccgg tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta
     3601 cttttcccag atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc
     3661 ctttttctca gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga
     3721 ccagggtaaa gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg
     3781 gtgatccctg cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt
     3841 gaaccggctg cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct
     3901 gcttgataag ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct
     3961 gacgttacac ctgtatcttg agacacagta gattcttccg cgattgtact agattctact
     4021 gttgaagtct cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc
     4081 gaagtttcag aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa
     4141 taagcacctg ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat
     4201 aatgtatttc ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta
     4261 tcgtagattg gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta
     4321 tcttttctgc tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa
     4381 agtgtcttgt cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg
     4441 tttcccaatg aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt
     4501 ttgctccatc aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag
     4561 ctaagatgaa ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc
     4621 ggaattcttt ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag
     4681 actgctggat cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg
     4741 catattccaa ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat
     4801 aatggatacg ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc
     4861 tgcctgctcg accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga
     4921 ccacataacg gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa
     4981 actgcagctg attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac
     5041 caccgtgata gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt
     5101 ctttttttgt tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa
     5161 gttcttgttc tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg
     5221 attgtctgat ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta
     5281 aatctgttgc tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt
     5341 ttcctaactg ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct
     5401 catctactac atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag
     5461 tcaacatttc gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc
     5521 ttctttcaga ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt
     5581 gtaattgcgt cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac
     5641 cttccattaa ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa
     5701 tagcgagagt atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa
     5761 atgaatcaaa tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt
     5821 ctcaatagat agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc
     5881 cattcgctaa agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt
     5941 tctgcagaga gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga
     6001 tgatctgtta ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga
     6061 ccattcataa acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat
     6121 gaatcgatca aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg
     6181 tggatacatc tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga
     6241 taaggaagca gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct
     6301 tcttctggta tctccgaaaa cagctgcacc aaactctcat acgcagtatc aattaaaatt
     6361 tcacgtggca attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt
     6421 gtctctgctg gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca
     6481 aatttaatga gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca
     6541 gtatgttcta tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca
     6601 ttttcattta tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta
     6661 tcctgcttca attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa
     6721 ataagtactg aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc
     6781 ttgtgtcctg atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag
     6841 gatggttaga aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc
     6901 tttgtcaaat cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg
     6961 cccaagaacc agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa
     7021 aagcgtatca aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa
     7081 cgaaacttta aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc
     7141 tcttgttccg tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg
     7201 actagttttt tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt
     7261 tttccaatgt attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat
     7321 ctcctttttg ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat
     7381 actctgttct ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt
     7441 tatcttttgt cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt
     7501 tgtcgggata acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat
     7561 tgacataacg acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat
     7621 ttggaaaatc ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg
     7681 gcattttgac aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat
     7741 aactttctgg ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat
     7801 tctttttctc tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca
     7861 cttgcgcttc tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac
     7921 cgagtaatcc taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct
     7981 cccacctttt ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa
     8041 aataagagag aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga
     8101 atatccgccc tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc
     8161 ataaatttcc attgctttat acatactact taattcccat cgttcattcg cctggtgcat
     8221 aaagtcagga gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc
     8281 aaaagtagcg ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt
     8341 ccccaataga tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc
     8401 aaattcttca taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc
     8461 atctttcttg aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt
     8521 gatttccaaa cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc
     8581 cacgacagct tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc
     8641 aaaatcaaat acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc
     8701 tgcatctttc gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa
     8761 atcgaattcg tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg
     8821 agctgcatca ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc
     8881 tggtcctgtc aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc
     8941 atctggcgca aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg
     9001 ccataaattt tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc
     9061 agcatcaatc aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg
     9121 ggatccgcga ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca
     9181 attattttcg tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc
     9241 tccagagcca atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc
     9301 tagcttttca ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg
     9361 atgtccttgt cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa
     9421 tgattctaat gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc
     9481 ctaatatgtt aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa
     9541 tgacgtttta agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa
     9601 aacactcact gaatggagct attgacttat ccacgtgctt atcgatagcc gtttatcggt
     9661 ttcttcattc ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct
     9721 acacctagga tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc
     9781 caccacactg aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt
     9841 ggtgtaatta agtttactaa accagatgca gattgataag cagtgattac taaatcctta
     9901 ccgacgccgg caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg
     9961 gaagttgaag gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat
    10021 acacttgaag ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt
    10081 aatccattgt tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg
    10141 atcaaatcag aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa
    10201 gtgattgcta ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag
    10261 tcacctaatg gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac
    10321 cagtttgtca atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa
    10381 ctgatgatca tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc
    10441 gtgattactt cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt
    10501 tggttgtata ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca
    10561 tatacaatag aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca
    10621 cccatcgaaa tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa
    10681 gctagacagc ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag
    10741 cctacagcca tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt
    10801 ccgtaagtag agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct
    10861 tttcctttga atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg
    10921 ataataccta aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt
    10981 gtttctttag ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa
    11041 atatcccaca gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg
    11101 cctgcatcgt ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact
    11161 gcaataataa taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt
    11221 tgttccatct tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta
    11281 ttttatcaaa aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc
    11341 aaaaagttcg tttttcccta ttccctcctc ttttttcagg tttttaaagc tttcttcttt
    11401 ttcattgtag cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga
    11461 atttcacatt tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac
    11521 aaacaaataa aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaaagact
    11581 aagaa
//
LOCUS       DACESX010000085        11460 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.085, whole genome shotgun sequence.
ACCESSION   DACESX010000085 DACESX010000000
VERSION     DACESX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.085"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(182..1222)
                     /locus_tag="IXF88_001908"
     CDS             complement(182..1222)
                     /locus_tag="IXF88_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1508634.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1571..1897)
                     /locus_tag="IXF88_001909"
     CDS             complement(1571..1897)
                     /locus_tag="IXF88_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508635.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(1884..2672)
                     /locus_tag="IXF88_001910"
     CDS             complement(1884..2672)
                     /locus_tag="IXF88_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1508636.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3040..3330)
                     /locus_tag="IXF88_001911"
     CDS             complement(3040..3330)
                     /locus_tag="IXF88_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1508637.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3466..3645)
                     /locus_tag="IXF88_001912"
     CDS             complement(3466..3645)
                     /locus_tag="IXF88_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508638.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(3642..3992)
                     /locus_tag="IXF88_001913"
     CDS             complement(3642..3992)
                     /locus_tag="IXF88_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508639.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(3985..5310)
                     /locus_tag="IXF88_001914"
     CDS             complement(3985..5310)
                     /locus_tag="IXF88_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1508640.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            5760..6374
                     /locus_tag="IXF88_001915"
     CDS             5760..6374
                     /locus_tag="IXF88_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1508641.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6539..6781)
                     /locus_tag="IXF88_001916"
                     /pseudo
     CDS             complement(6539..6781)
                     /locus_tag="IXF88_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6985..8304)
                     /locus_tag="IXF88_001917"
     CDS             complement(6985..8304)
                     /locus_tag="IXF88_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ1508642.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(8459..9046)
                     /locus_tag="IXF88_001918"
                     /pseudo
     CDS             complement(8459..9046)
                     /locus_tag="IXF88_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9684..9941)
                     /locus_tag="IXF88_001919"
     CDS             complement(9684..9941)
                     /locus_tag="IXF88_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1508643.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(9934..10203)
                     /locus_tag="IXF88_001920"
     CDS             complement(9934..10203)
                     /locus_tag="IXF88_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1508644.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(10334..10513)
                     /locus_tag="IXF88_001921"
     CDS             complement(10334..10513)
                     /locus_tag="IXF88_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508645.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(10894..>11460)
                     /locus_tag="IXF88_001922"
     CDS             complement(10894..>11460)
                     /locus_tag="IXF88_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508646.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         3458 a         2107 c         1740 g         4155 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaatcataa aaaagttcta atttaaaatt gatcattttt atagtgatcg ctttttatca
      181 actactctag ccaattttcc gtactaactg ttggtaattc gttaaatgtt tctctttgtt
      241 cgtttgcaag ctttgccgca atagcttttt cgacaaacca attttttaca taaacgaaaa
      301 ttagattctg caaattgttt actttttcag ttttttgttt ttgataagct ttcagcaaag
      361 tcatatataa tcctttgtca gcatcaattc cataagattc cagttcttca taaacaacaa
      421 ctgtttgctc taattcttca gcttttttct ttgcattatg aatagtttgg acggtttttt
      481 gagcgtcttc gtaagtacta gaaaatgttt ttatcaagtt taatacattt tctggtacaa
      541 atgtatttat actgtcctct ttcatgatgt cgacgaagtt atctagtaaa acttgatttt
      601 gtaactgatc agtttcagtg tctattatgt gtctattagt gtctatagaa ttagaataga
      661 atagattgtc tgcgattttc gcaattacgg ttgagtcctt gatatgactg gattttgccg
      721 tatttgcgat tttcgcaatt acggtggtat cattgatttc tcgcggtttt gccgtatttg
      781 cgattttcgc aattacggtg gtatcagtat tgtcttcctc ttccatgatt ttaaagacgt
      841 cttgattcgt tacttctggc ttgcctaagt acaataaatt aggcatggat tcaatcttac
      901 cgtcacgttt atttactctt cctctcttct gtttcagaag acctgctttt tctaattcct
      961 ttttgatttt agatacttta ccttcgcggc attgtaacaa tttcattaat tcttcaactg
     1021 tgaatatgaa aaatatatta tcatcagaat ctacccagtt atttttgact gaataatcaa
     1081 atcgatcttt aagtagcata taagcaattt ttgcatcatt tgatagatcc atgtattttt
     1141 cgttagtaaa aaatacctta ggcatttgat aatatttttc acgataaatt tcttttgatt
     1201 tgtaataatt aaaatcattc atagccaatc ctctttctgt aggatagcgt tttgttcaaa
     1261 actatgttac aatgaattaa acaaatatat ctatccacta tatatttttc tactgctgat
     1321 aagtcgccaa actttgttaa agcagtagtc tttttttatc tttttttagt ttaccttaaa
     1381 aaataaatta aagcaaaccg ctctatttca aggtttaagc tcatagtaac tatatcacga
     1441 tattacaata atattatatc acgatatcgt aatattacaa tatgaagata agcgacttat
     1501 cagctttgat actactgatt tttataaaaa aaatatcacg atattatgat atcgtgatat
     1561 tgaatgtaaa ttaaatttta ttttgtttct ttgctagttt atatccttca atcaagtatt
     1621 cttcaggtat ggactttaat agcgcatctt ctataacgtc tggtttactt ttgtctgatt
     1681 cagcaatagt cgtccaatat ttgagtgctg acattagaat tggatttata cgaatgctat
     1741 ctgctgttct tacactactt ccttgatctt ttatgacttt acttatattt ttcttggtta
     1801 attcgatttc ttctacttct tttgatttaa aattcttttt atttttattt cctagtaatc
     1861 tacttactgc agcttgactg cgattatctt ctgccatcaa aatcaccaac ttcttttaaa
     1921 tattcttctg taaatgcttt atagtcatct tcagctgttg agggtttatt aaaattgatt
     1981 agatctgatc cagcagcttg cgcttcgtca acggttattg tcctacgaat ataagttttg
     2041 aaaatttttg caccaatgac tctagaaatt tcggttgctg cagacgtcat ttctttagaa
     2101 atgttataac gcttattata cttagtaaat agaactcctc gtacttcaag cttgtcgttc
     2161 gtatattctt ttaccgtttc aatagtttca cttaacttta ttagtccttt taatgagaaa
     2221 tacgcagctt caactggaat gataatttca tcagaaacag taaatgcgtt gactgtaagc
     2281 attcctaaac ttggtggaca atcaattata atataatcat agttgtcctt gattggttga
     2341 agtactcttt taagcctgtg ttcacttcct acttgagtaa attccctctc tgcagaagaa
     2401 agggctatgt cggctggcaa aacatcaatt cctttatagt ccagaatagc atctgtaatg
     2461 tcagcctccc cttttaatac ttcataaatt gtaggttggt ttgttccatc tatatcgaaa
     2521 cctacattat ctgtagcatt tccctgagga tcaatgtcca ctaaaagtac atttttaccg
     2581 taaacaaatt tcaaacatgc tgctaaatta acgctagtcg tagttttaga aactccacct
     2641 ttttgattgg ctactgacaa tattcttgcc attaaaatat cccccattca tcttatatta
     2701 taatataatt atatcataat attgtgatat tgcaatatcg tatatgcatt atattgtaat
     2761 attgtgtgat tattttgttt taaagatata tatcggttcg gatataaaga ttattcatat
     2821 gttattcgaa attaaaataa tcacttttta ttactttaaa attttttatt aatgataaga
     2881 aatatgtttt gcgagcagca actagaataa tttaaacatt actaaactgt ctatattgtt
     2941 actaataatt cccttcttat agtgcatttt cttagaaaaa aacaatatta cgatatcatg
     3001 atattaagat atcgtaatat tatacaattg tgattgatat tatatatgtt tttttatttt
     3061 tttatcatgt ttaatcttga aggaatcata ttctttttct aaagacagga gcttttcata
     3121 aacatcagac atgtttaaac tagaggccag gcttaaatca taaacgaatg aagcaggtaa
     3181 tgattttatt ctacgttgtc gggttatcca gcttgcaacc gttccttgtg ggtaggcata
     3241 taactcacaa aattgttcta cagtgattcc taaattcttg ataatgtaaa cattgatcgg
     3301 gtgcgagtaa acaaatgtat tgatagacat ggtgcgtaag cctactttct tttattattg
     3361 cgtttgcaat aatagtttaa cacacgcatt attctagtat ctactcctat ttgtaaaaac
     3421 aaaaaaggat tcatagagat aactctatag aatccgtaaa ataccttata tatataacgg
     3481 gaagatataa aacctaaaat tatgataacc tgctgcagct tctacataga aagagaagcg
     3541 ctcaacacca acctcattta tcaaacgttg gccataaccc caaatctctt ctgatagctg
     3601 atcaaaggtt atatacttta gctcatattg ctggataatg ttcattctaa tcgactccat
     3661 ttattatcct ggtaaaattc gatatgacgt atttcgtcgt agtgaatttt gtcttttcca
     3721 acgtagatcc ccagctcatc actacctaag atcttgccaa tgatatcttc tggatagtat
     3781 ccttcagcgt ttcgttcttc tttttgaata gatactgatc tattttttat gactgcagcg
     3841 ttcaatactt ccgcaatctc ttcagtcgtc atttgttctt ttgctaaatt ttgactggtt
     3901 cgttgttcat tgtcggcatc aatttcttca gtatgatcag ataaataaaa ccctgcccac
     3961 tttaaacgtc cacgatcatt gtaatcaatc atttttaatt cccccatttc cgcccgcatg
     4021 accaccaaca agtgtgcttc tagtaatgct tctagcgcct tctaacattg aagaagcatg
     4081 tacaatcgac gtaaaaccat atttttgccg aatcttgtca ataattttat ctaaattttc
     4141 ttcgtcaatt tgcttttgtg gatcactaaa taaatccagc tgaagcgaat cggtgtacac
     4201 aagcttgcta taggttattc caacatgcct tacttcttgt ccttcataat acttatcaaa
     4261 caagaaaaga caataattct gcaattcctt tgtattatct gtgataggga tcttcatctg
     4321 gtgtgaaaac cctggtctcg tttctaagat cgacgtgcca atgtgtaaat ggacacaacc
     4381 tgttttacag ttatgctgcc taatgcgtat cgctacttgt tccgccattt cttttaccac
     4441 cagctcgatt tcatttcttc tagtataatc tcgtggtaac acttgtgaat ttccatatga
     4501 tttttccttt gtcggttctg gtgggattgc tatatctgtt cgatcgatcc cattagcatg
     4561 gaagtacagc tgtaagccaa tcacacctag tctattctta atcgtataag gattccagtt
     4621 agcaagatct ctaatgctca taatccccat ctgattgagt ctttttttca ttcgagagcc
     4681 aattccccaa aagtccgtca tttcaggaat attccatact ttttcaggaa cgttttcgta
     4741 cgtccattcc gcaacaaatc cttcattatg ctttgcttcg ttgtctaagg ctagtttagc
     4801 caaaagggga ttatctccga cacctactgt agcaatcaat cctagctctt ctttaatatt
     4861 ctcctggatc atttgagcca gcttctttct ccgttgactt cgtgttcctt cagtcgtaaa
     4921 aagattcagt gatcgggtca cttttaagat tgattcatcg atcgagtaaa tcagtagatc
     4981 ttcatcagcc acatatcttc tgaaaatatt atttacctgc atatttcgct tgatatatag
     5041 gttcatacgt ggtggaacaa cgtgtagtgt tttaggaaat ggttgtggta agtcacgtgg
     5101 tctactcaca tttgtaatac cataccgctt ttttgcttca ggagaagaag ctaatatcaa
     5161 tcctgaacca gtattatcag ctcgactcat aacaacaagt tctgttgtta atggatctaa
     5221 atttctttct atacattcga cacttgcgta aaagcttttc atatcgatta aaaagtaatc
     5281 atttactggc tcttttgaat aatccatcat aaataacact ccattctttc acattataca
     5341 aacatatgtt cgatattgta aagaagaaat agctaaatac ataaaaaaag aagcataggt
     5401 atgctatcac cctgcttctt cttttgattt gtatgatcaa tttattctat cactttacta
     5461 attaatttga aaatctaata aaaactctca ctctaaaaga gtgagagact gcaaattaga
     5521 gtacggactt ttttggagtt ccgttcttta atattaacaa atgaataaaa tccttacaac
     5581 atttgagaat aagttatcag tagacactta caaaaaattc tcatactatt tcctccatca
     5641 ttagtttttg tctatataag gtttacccta aatagacact ggtataacat tgaaaaatat
     5701 ggcttcctcg tgtatgatta gggtgtattt taaatagttg cagaaagtag gtaaaagttt
     5761 tgagagttgc atacatacgt gtctcatcga tcgatcaaaa tgaacaacgc cagattgaag
     5821 aaatgaaaaa attcggtgca gagagaattt tcatcgagaa acaatctggc gcaacgatta
     5881 ctcatcgacc tgttttccaa gaagcattag attacgtccg agatcaagat atttttattg
     5941 tggaagctat tgatcgactt ggaagaaatt atgacgagat catcgcttca gtcaattact
     6001 tgaagaagaa aaatgtgaag ctgatcatta ctagtttacc gatcatggct gaagcaattg
     6061 gcaatcctct cctggataaa ttcattaagg atctgatcat ccaaattttg gccatgatcg
     6121 ctgaacaaga acggactgaa tcgaaacgtc gccaggcaca aggaattaag atcgcaaaag
     6181 caaacggcgt ctacaaaggg cggcccaaat tatacagtgc agacgcaaaa gatccccaac
     6241 gtcgccttgt ttacaaaagc atcgttgaag atcttaaaaa tggtgttgcg attgctaaga
     6301 ttgctaaaga ctacaatgtt acgagacaaa ccgtgtatag aataaaaaaa gacagcatgg
     6361 tcaatgacaa atagttgatc atgctgcttt tattatcttc tcatctttaa tttcctacat
     6421 ataattacta tctataaaat taagtcttac tcagaacaga taaagcataa aattccaaca
     6481 aaccattttt tacttaacta aataaagagt ctgttaatat aatcttcact tatttacgtt
     6541 aatcccctac tgtatttgtt ggttccttat ctatattttc tacaaaatcc ttattatctt
     6601 gtgatttagc ttcgtataat tctttgtatg tcgacacatt ggttatacta attcctatag
     6661 ctagttcttg agactctact tttctctcta gctcctttga caaagtgatt tgagatttat
     6721 ttggttccgt tggagtcaat tctatagatt cttcataaaa atctctatac ataaagctat
     6781 ccctgaataa ttcatagctt taattctttg gcacatttta ttgaaatatg tatcactaat
     6841 atccctatca actgattttg actaaaaagt tacttcaaac attcactgtt caaaaatatt
     6901 gaggaataaa ttttgggtgt aaggcatttt gagcatactt ctccctggat aacttcccag
     6961 taaaaaaatc gttagattgt tggattagat ccattgactg gctcgcctca ggcgagcaaa
     7021 gaccctgtag aattcattct gatacacatg ataactagat aatttgagat agactcgtct
     7081 acccgtttgg actaatttcc cagcaacttt cagaaacttc aatcgaatag aatgaatggt
     7141 cattcccttt tggacttctt caaagccaat cgttcttaag aagttgacta agttgtaagc
     7201 tatcaagctg agcatcattc ggacatgatt ctccaaaaag cgaggactgt ctgttttgtc
     7261 aaagtaaaag ccagctttcg cttctttaat gaagttctcc attgtgccac gtttggcata
     7321 gatagaaaag atggtatcag gagaaacatt ttctgataga ttcgtcacga taaattcatg
     7381 tcgaaagagt agttcgcccg cttcacgtgt tgagcgtata cacacgcgac gactttgtga
     7441 ccaggattgt gcttgataag tggtcgagaa gtactgaact tctcgttctt cccacttttg
     7501 attatcgcca taaagtactg atttctcagc tatttgacct agtctacgat tattcttcag
     7561 tcgaataaca taatgactct tttttgattc acacgaatca tacacaccag gtgtagcgaa
     7621 cccgctgtct ccacgaacca agatgtcagt gtttggtaga gagtgattat agtgctctaa
     7681 taaaggtgtg agaaactcct ttacgccttt tgatgtatat tgatttcctg aacgtagttc
     7741 agcttttaag aagtcaccag tcaatccgtc aaaagctacc aatggatggt aaccataggt
     7801 ttggtagtgt gtattataat tcgtttgttc ttggtgacca aatgtatctg aatgggtcga
     7861 atctaaatca atgattaatt ccgtatcatt acggatgagg cgtgctttat cgataagttc
     7921 ttggttcaaa gcttgaagtt catggatatt ctcctcagat agtcgatcta aaaatcggga
     7981 aagtgaagat tgagaagcga gttctttcct atctaaaaca gctttaaaca caggatcttg
     8041 tctcaggaga ttagctgcag aatcagctga gtaaccagca attaattgca taatgaactg
     8101 ctcaagtatg gataagttgt catgagtaaa atacgctcgt tcgtcttcaa tgtgaatgtg
     8161 ttgcttagcc agttcagaaa aaccaaagat gttcatcagc tctttcacta ggacgagacc
     8221 cgaatcactc gataattgac cacctgtatg agaaatagtg atatttgaat tgaattttac
     8281 ttggtttttg tgtaagctag tcatgaaaag aactcctttc ttttggttgg tttagtcact
     8341 ttaaccatag cagaaagggg ttcttttttc atcacttaac gggtgaagat gaaaaagtaa
     8401 ttgtaagctg ccgtgaggca gtttcacatg gttttaatta aaagtatgaa ttattcaggc
     8461 tatcattaat tttataaaaa aaattaaatg tttttagcac tgccatataa cattcaaaac
     8521 attcttctat agttggttta cttccttgag gagaatgaac caatttatta cgtttttcta
     8581 tgaaattttt aatttccttt cttatttctt tcggaaactc aatattaaag taccctttaa
     8641 acatgctccc taataatttg tctacagggg gagattgtaa attctcaaat attagagtta
     8701 aatcaggttt ctcttgttga taaaattcct ttacccctat ttcacaggct gtaattatcg
     8761 aaataaacct atctctagta tttgatatat ttttacttct tttaaggagt ataagagaaa
     8821 aggggatact ttcgtgtcca tgcatgtaat taactaattg ttgagcttca gaagttatat
     8881 ctctaattat atagttaacg gaattaggaa gtgtctttaa ctctaatgaa atttctatcc
     8941 aattactatc ttctaatctg tatatgctcg aagtataatt tgattcgtca gcaagcatcg
     9001 ctacatccca aacactgatc ataaaatctt ttaaacgttc aatcatttca gcaatattgc
     9061 taaaatcagc taatttttct ttagaataaa aaaaaacatt tatatcagaa tcaataaatt
     9121 ctattttttt attactatat cgtcatcatt atcttgacca tttatgttta ttgttccctg
     9181 agtggggagt acttctttca aattcaagtt aaatttatat tccattactt ctcctccaat
     9241 atataaaaat tacttaactg tatccaaatt taatatgtaa ataaatcatt aatttttagc
     9301 taaattctat gagaagttta tttttgttgt ttttgctaat taactaaaac tagtacaaaa
     9361 aatttaaaat agattattaa acctattgct tattctattc agtcatatat ataagcgtca
     9421 tcctttagta actaacatcc cacactacaa tagtattata acatatctat tcaacttcaa
     9481 attgttgaaa tttaagccat ttatatttac ttttcctcaa ctttagaaat gattttacta
     9541 accttatatc cgtaatacgc gtaacaaaca cattcattaa taacaatcaa cactaagcta
     9601 tattagcttt ttaaatgact agggtaaata aaataaggat caaaaacttt taagtttctg
     9661 accctttcct tacttccgat tggttaatag tgatcttttg cagaataaat aaatatcgtt
     9721 tcattttcaa ctctatatat cagtctatgt tcatctgtaa ttcttctgga ccattttcca
     9781 gataaatcat gctttaatgg ctcaggtttt cctaatccag caaaggggga acgatcgata
     9841 tcttttatta acttgttaat cttttttata ttgcttttgt ttccttgctc atgccaataa
     9901 agataatcat cccaagcatc atcagaccaa gccttaatca tcagattcaa cctcgattaa
     9961 gtcatgtgtt ttaaatgcac ctttggagaa ttgttcatct cctcgacgaa ttttttccat
    10021 gacgtaatta ttagaaagtg ttctcaacgt ttcttgcata gaatcataat ctctttttga
    10081 taatacaaca actgtatctt ctacatcttt acttgttaca ataagtgttt cagcatcctc
    10141 attaacttgt ttcatataac tacgtaaatt ttggcggaaa tttgaataag ctactgcttc
    10201 cattcctttc accccttatt tatttctata aaacaattgt acattatatt gtacaattaa
    10261 gcaaatgatt aatattctaa cttcttataa ttaaaagtgc tatcactttt agtttttagg
    10321 ataaaaagtg acgtcacttt ttgagtgctg gtacctcaaa tcctgctttt tcttgggcaa
    10381 tcaataaatc taacgcaata cacataatcc tggattcact tttgccgtac ttctgcttta
    10441 gttgctgcaa ttctttttgt ctcttttcat ccaacgtaac gggtaaccgt ttatttccta
    10501 ttgcaattgg cattagtact ctctcctttc atcgaaataa ccctatgaaa aatatggctg
    10561 caatcatctt gaatagtaga ctcacaaggg ctaatacaaa gcgccaaaag atcaaacaaa
    10621 ataccagtat tgccaaaaat actacgaagt ttaaatgcac agctgcagca tggcgcataa
    10681 gtatttcgcc aacaaatagg actaacaata gaggaatgat tttattcata tggatctcct
    10741 tctttctctt aggttatcgg gtgttgcccg cttatttcgc accacctaag cggcgggggc
    10801 ggttggagcg attggtgcga aataagaacc attcccttag ttactaggtt ttttgtcctt
    10861 atttcacaca ttctcacttt tcggtataaa tgattattgc ctttttttcc tttcaaatcg
    10921 ttgatatggc attcgtttac ggtcaagttc attttggatt tcttctgtat cgtcgtaagt
    10981 ataggtgtct aaaaaatcag ttaaagtcgt gtaccagtcg tcgcctgggt tatctacttc
    11041 ttctacatat tccctcaaaa atgcttcggg tacagcgagt tcataatcca ttcccattaa
    11101 cacgatagat tccgttccac acgtcaaaaa tctagtaaat acaaggtaat aatcgtctat
    11161 cgtcgcatct ttgctgttta gctcttccat ttcttcttcc attaattcca tgctctcaaa
    11221 aattgaataa tattctgatt ctgagtaata gtgtttcctg caaatcgtgt agctgacaaa
    11281 gttttcgacc agctcactcc tttctaggta aacattcatg atgtattttt ctgtttctag
    11341 tcgtagaagc tccgttcctt tttttaccgt ttcaggacga cgtttataca cgtctaaacg
    11401 attatggctg actgtgacct tcgtttgact cgtttcactt tccaaataca attgttcttc
//
LOCUS       DACESX010000086        11224 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.086, whole genome shotgun sequence.
ACCESSION   DACESX010000086 DACESX010000000
VERSION     DACESX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.086"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            39..887
                     /locus_tag="IXF88_001923"
     CDS             39..887
                     /locus_tag="IXF88_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1508647.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1533..1739
                     /locus_tag="IXF88_001924"
     CDS             1533..1739
                     /locus_tag="IXF88_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1508648.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(1941..2942)
                     /locus_tag="IXF88_001925"
     CDS             complement(1941..2942)
                     /locus_tag="IXF88_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1508649.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(2947..4860)
                     /locus_tag="IXF88_001926"
     CDS             complement(2947..4860)
                     /locus_tag="IXF88_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1508650.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(5028..5534)
                     /locus_tag="IXF88_001927"
     CDS             complement(5028..5534)
                     /locus_tag="IXF88_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1508651.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            5694..6134
                     /locus_tag="IXF88_001928"
     CDS             5694..6134
                     /locus_tag="IXF88_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1508652.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(6160..7317)
                     /locus_tag="IXF88_001929"
     CDS             complement(6160..7317)
                     /locus_tag="IXF88_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508653.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7320..7676)
                     /locus_tag="IXF88_001930"
     CDS             complement(7320..7676)
                     /locus_tag="IXF88_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508654.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(7974..8948)
                     /gene="sgrA"
                     /locus_tag="IXF88_001931"
     CDS             complement(7974..8948)
                     /gene="sgrA"
                     /locus_tag="IXF88_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1508655.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9144..9983)
                     /locus_tag="IXF88_001932"
     CDS             complement(9144..9983)
                     /locus_tag="IXF88_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1508656.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10168..11055
                     /locus_tag="IXF88_001933"
     CDS             10168..11055
                     /locus_tag="IXF88_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1508657.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         3431 a         2199 c         1709 g         3885 t
ORIGIN      
        1 aaaaaatgat aatcttatta tgaaaatgag gaattctcat gagcgtctcc ataagactac
       61 aaatcaaaac aatgtacaac gacttcagcc caaaagaaca ggctatcgcc gattatattt
      121 tagaaaatcc aagcaaagta agtcatagtc ctattagcaa tttagcaagt gaactgggca
      181 ttgctgactc tacttttttt caatttacaa aaaaacttgg ttataatgga tttaaagatt
      241 ttaaaatggc catgttgatc caagaaaatg atctatctgc tatttctatc cacgaaaata
      301 ttcagaaaac agatactgag ctcactatgg cgcaaaaggt gttcgagtct agtatttcaa
      361 ctttaaatga cacaaaaaag ttgttacaac aaaaggatct aaaaaaagcg gctactatta
      421 tcaatgaatc taaacgtgtc tattttttcg gtgttggcgg ttcagagatt gtagcaactg
      481 acgcttatca taaatttttg cgttccccta tcgcaacttc tcacagcacg gattaccata
      541 ttcaattgat ggaagcttct ttattgacgg aaaaagactg tgctgttttg atttctcaca
      601 cagggcaatc aaaagaaaca atccatattg ctgaaaccgt aagaaaaact ggagcaaaaa
      661 caatcgttat tactagccaa gctaattctt cccttgctaa acttggcgat gtcgttttta
      721 tttctatttc tgaagaaaca gaattccgat ctgaagcttt agcttcccgt atttctcaac
      781 taagcatttt ggactctcta tatgtgattt tgatgtttta caacgaaaaa gaatcacgtc
      841 gttctatcgc taaagttcga catactattt ctgaaatcaa agaatagcac cataacagaa
      901 aaagcgaaaa atccataaaa caaaaaaagc cactaccaaa tatttggtag tggctttttt
      961 ggttctgcaa gggtggtaca gaacatcaat agtcggtggg ttagaacgta tgtcatcgca
     1021 tctttggaga aggttgtatt ggtattattc gagttacttg ttagatgcga taacaaagtt
     1081 agtatacagt agtaaaaaat ttatcttttc ttttttttat tatttttgtt tcttattttc
     1141 taaaaaatta aaaaaaattt tggtgaatta agtatttcct tgataacact ggcttttggc
     1201 ctcattttag gttatcattt gtaattttat ttcacagttg atatgattgt tatgtaaaat
     1261 aaaaaagttt aggatacatc gaatttttgg ggaaattctt ttgggggtat cctaaatctt
     1321 ttctattata ttcccatatt ttctattatt ggtctagacg tatgatggta aactttgtac
     1381 ctatctgatt gctgaatcat taattgctta tcattagcca tctttgtttc aagttcgcta
     1441 gatgatcatt ttattcacat cctaacaaac ttgtcacatt tatagattct taatctattt
     1501 atcatacaat tataaaaaag gaggtgctgc ttatgaaagc tatccatatt tctttgcttt
     1561 ttttcgctag tgcattgatt ttatttgcta ttcatctttg ttcccagcct tatatcaata
     1621 acaacggaat attagtggaa ccagcattct tttgcttacc actcggcttt ttgtcccttc
     1681 ttgcctccgc aggcttcgga tttgttgctt tttttaaacg atcaaaacaa caaatctgac
     1741 ttataaaatt tacgtaaaag agatcgtgac aataatcgtg tgatattccc aaatttcgaa
     1801 taaaaggtgc cccttgccat ctctaactcc tcagtcacaa gttacaatat tcataaacca
     1861 tgaaaagctt gtttattgaa attgctcctt gtgacttgga gctaagtgac agattacaac
     1921 ctcttttgca tcataagact ttactccttg aaaaacatta ctaacttttg atatgtttct
     1981 tgcgcttgtt tattgctgaa atcaaactga tattcatggt tcaaaacagc atttttctcc
     2041 gcataatcta cttttgtgac tttcaccccc gctttctcaa atacagaagt tacttcttcc
     2101 atttgtttcg taaacgtatt tgtatttcca tccgttaaga atgtgttttg cgtccaacga
     2161 tccgcatgtc tgaccaaggc taaacttttg accttctctg attgttcaaa gtccttatta
     2221 tcaaagtaag cccatccaca tttttcataa agaaagctag agattttatc acttacttga
     2281 gtaagtgcag aaggatccaa cagaccagac aaagaaataa acttcgtaat ctgcttgttc
     2341 tccaaagtcg gatcaagatg atcagctttt ttaatttctt cattttgaag tgctaacaaa
     2401 tattctccac taatttgtgc acctgccgaa tttccaccaa tcgaaatatt cgaccaatct
     2461 gcttgatctt ttatctcttt tttagctgct tgaatggctt cgttcaattg gataactggt
     2521 gtaggatgtt ttgcttccgg tgatcgttca taatctatat ttacgatgat ttgctgcgtg
     2581 tctgaacata gatattccaa ataatcacga caatcatttt tatcccctcc tacatatgcg
     2641 cctccatgca tccagaaaag aatcggtaat tttttctttt ccactttcgg tgtgtagata
     2701 tccatattat tatttttgta ctttgaagga taggatattt ctttttttat atcgatttgt
     2761 tcgatttttt gctggaaatc atctggtcgg gcatatactt gttctttttc acttgtgtca
     2821 aattgttttc taacaagcaa gctaatcggc tttggtgtga tttgattaat caaaaataca
     2881 gtgactaaaa taactacgat gataactggt actgcaatca ctattcgctt gaaccatttc
     2941 attcatttac cctctctttc ttcgccaaat ccatgtgata atcgtgctaa cagataaaaa
     3001 tccacctgaa taaatgagaa gcgaatgttt cttttctcca gttttcggca acattctttt
     3061 ttttgcatct gtcttttttt gtactttcac tgtcatctga atcggttgcg tatcatcact
     3121 tgattgatcc tctcgaggtt cattctttcc cttattgtct gcattctctg tcggatcttg
     3181 cttttttggt ggagctactg ggattttttt gtaatagaaa ataatagttg gtatcgtctc
     3241 attaaaaatc ccttcagtcg gtccttcaac atttgctaat tcgtatcctt cgaatgtctt
     3301 ttgctctgtc atataatttt ctccataagc tccgtaaagc tggatttcgt cctggtcttt
     3361 gagtagactt ttattgtttt catccacata ctttacgttt atctgatcat ggaacactac
     3421 tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg tcaacttcct gtgcctttcc
     3481 atgccctgct ccttctacta ataaaagatc tttataccct cctgataact catataattt
     3541 ttgcgcgtta gacacaggta ctacatcatc tgctgttcca tggataatca atttaggcaa
     3601 tgaatctgac attccaatcg taggtagagc agcttttgta tccaaatgca tctcctgatc
     3661 aaaatagtat tgtttcaaaa ggcccataaa taaatcttca tgctcttgat ccccaatgat
     3721 gtctaactga ttttttatgg catccggtag tgcgggggct actgtctggc tgtacgtaaa
     3781 acgtagttgt tcatcaattg atgcaaatcc gcaattttca ataattcctt tgacattgac
     3841 aggaatatct ttcgctaata cagacatcat cgtagctgct cccatcgaac caccataaag
     3901 taaaatcttt tgagatggcc gctctcgaac ttcttgattt atccagtaaa ggacgtcatc
     3961 tgactcatat tgtccatagg tcacataatc gcctccgcta ttaccagtcg ctctggaatc
     4021 aacaaataat aaattatacc cggccttata aaaatcattg tattcttctg taacgatccc
     4081 gttattccaa ttccctcgaa atccgccatg gatcatgact gttttatctg aaccttgatc
     4141 gacataatat gcatatacat ccatcatttt gccatcttgt ttgtttagta cagtttgatg
     4201 aagttgtttt gtatttggat agaagcctgt gttttctgta ggcgttgtcc catcatctgt
     4261 acctccaagc atcgacatca acacactgtt ttttgcaaaa gcgatatcgt acaatagata
     4321 agtagcaatc agatgtttga taatctcttt atctacacca taatcattaa cagcaagctc
     4381 agtgactaat aactctatta attttgactt gatgtcttct gttggtccat taagtatttc
     4441 tgtcatctgt ttaccgaagt aattcaaata ttctttacca ttgaaatttt cacctttacc
     4501 gatatcatca ttttctgtcc cattctcttt gtcacttact ttagcagaag aggattcgtc
     4561 gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg ttatctgtgg tagcggtcgt
     4621 ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt tcagtatttg tttctgtttg
     4681 tgaagtactc gatttcgttt catttccagc atcagtagtt tctgtggttt ctacctcccg
     4741 agattccgat tcggaagaaa tgctctctcc atatactgtc cattgcggga cgcttgtact
     4801 tacagcaaat aagatgacca acgctccctt taatacttgt tttgttctca agctattcac
     4861 tcctttttat gtttctagga gaattatatg taaacgcaaa cattttttat acaaaaaata
     4921 aatggtttat cccataaaaa tagaacagga ttattttctc attttacgta ataaagcaga
     4981 atggataaac cagtatccat tctgcttttt ttaaacgaag aaacaactta ttgctccaaa
     5041 aatgtcagaa atcgtttatc ccaaattttc tcgtaaaaag cgattgtttc ctttgctttc
     5101 ataaaaggat tatcataagt tgtcgttgct cctctacaca tcataagctg gtatcctaat
     5161 ccatgagcaa acttcacagt cgtatctata caatactctg cttgtgcccc acataattcg
     5221 atcgaatgac agtccaattc agttaataaa acactcaaat tagtcttata aaatgaattc
     5281 gcatgggttt ttctcacaaa ataactgtgc tcaggaatag ataattccga caatagctcc
     5341 catggttttg tttgtggcac taattgagca tcttcgtgtt gaacgaaaat aactggtttc
     5401 ccaatcgttt cgtatgtttt gattctttta ttcactaatg ctgtcactgt ttctaattcg
     5461 tgtatcggcg aagaatcaaa acatacccct ttttgtaaat cgatcactac tattgcgtcg
     5521 gctgtgtttg acatacttgt gccttctttc ttttttcttg atttaatagt atttcttatc
     5581 tagctagttc cattatacgt gacaacattt tcttatttta cacctctaaa tggcactttt
     5641 cgactatttc ttcatgttat tatttcataa atacttagct attggaggaa tgtatgatga
     5701 ttaaattgta tacaggttct ggctgctcgt cttcaagacg cgcaaaacgt tggcttatcg
     5761 tccatgatat accgtttatc gaacagaact taattcgtca accactaacc aaaaatgaat
     5821 ttatccactt actaaaattg acaaacaacg gcgtgaccga aatgctctct actcattcta
     5881 ttagttataa aaatcttgtt cacagaatag atgaactcag tttgagtgaa ctatatattg
     5941 agattcgtac atccccttct ttattgaaaa aaccgattat ctacgaccaa tctgcaaaac
     6001 ttttgactgg atttagcgaa gataaaatca ggatgttcct ttctaaaact aaaagaaagc
     6061 aagagctcaa cgaactcttg ctttctggtc atcttgtcga ttttaaagaa aataatcttt
     6121 ttgcaatgtc ttaattttgg cataaacaat ctgcttttct taattaacga aatccaattt
     6181 ttccaatatg gatctatttt tctcattcct gatttttatg atttcagctt tcagacgcgc
     6241 ccaatcattt tttgtaggtt gtccattcca gtaaaatatc tgtgtattcg tttgatcata
     6301 tgcctttttg tcgataggat aatcgctaat gacaatatca ggtaactgat cagcggatgc
     6361 actatatttt attgggcgtt cagtcataga taatatcctt ttctttgccc ataattcctg
     6421 tgttttacca tactttgatt gaatatagaa tatgacaggt tgttcgtgtt ttataaaaat
     6481 atccctgact aacatacagt attgaaaaat caaccgtctg ttcttttcat aataagtatt
     6541 aagctctgga attctgcgag ctaccccact aaaaaactgt tgcatgattg gatacatcac
     6601 tggaaatata tgctgaaaat ctctgtcttc tgctgatttt ccaaaagcat ccaccttctt
     6661 ttttgtccca aaaacccttg aatacaaatg gatcattgcc aaattgacat atagataatt
     6721 tttttctgaa gttgtaaaac aaatcttgaa gtttttttca aaattttcta caatagacga
     6781 ttcaatccag ccccaacttt cttgtacttt atctggagta tccaacaaat cttcttccaa
     6841 ataggtgttc attacaccaa acataaaata aaggtaagct tgctctgttg gagtgatgtg
     6901 attcatcgac cagaagtttg cttcaaaaaa aggctctagt atgtaatgcg taaactcttc
     6961 ttttgttatc aatggatgca caaatttgtt catctcttct gggacatacg taatcgagtg
     7021 gccttgtttc attcgttgca acgaaattct tagtaggctg atccatttct ttctatctat
     7081 actacgcgta tatggcgcat gtttcgcaaa tttttctaat agttgacgtt tattttttat
     7141 acttgataat atatcagaat gattttcgtc gaatgactgc caatacatca agtgaaaaaa
     7201 gtatcggata cggtattctt ctccttctat cggattacct tttttcaagc tgcaagataa
     7261 atgatagttt ttcaaaatag tattaagttc tcttaatttt ctttgaatcg tacgcaaact
     7321 tatataattt tttgactaag attttctaag gattgataag tacctttgta aagtgaatcc
     7381 agtaaaatat aaccaatcgt tccctctaaa agtatagaat aaactttact cagtaaaaac
     7441 tgagaagaaa actcgacttt tatcatcttg ttttctgaaa gaaaagataa tttatattct
     7501 ggataccttt cgcaaaacga ttctgcttgt tgaatcaccg aattcatctt agccagggtg
     7561 tagtttagat ctaaagcgat atccttcacc acatataatt tctcgtctct ttcataacaa
     7621 tagttcaaaa attgatatac ttgcctttca ttgatatcaa taaattcttc aaacaaagta
     7681 catactcctt tttatctatt atttagactt attccgacag ctacatgaat aacgtacaaa
     7741 tagattcgtt actgattttt cagagatact accatgcgca gtgtgcattg tcaattattc
     7801 ttgctcttat tttccatttg tgttcctgtt atttcctgtt tctcaaatat gttagtaaac
     7861 aaaaatagat ctggcaaagc tacaacttcg ccaggcaata cagcactctc gaatgaaata
     7921 ttcactcaaa aatctatcgt tttattcaat tttagatctg ttgcttttat tctttatttc
     7981 actcgatgga agagagaacg aaaatagctt gctcctacaa tcaaaatgag taacacaccc
     8041 atccatgtaa ttagcttgtt caaggttcta ctaccagttt ctggtaaatc gctttttcta
     8101 ttatctgttt ctttcgcttt ttcttgatga tcaattccac gagaaacaaa atcaggctgt
     8161 tttagcacac tttttaggtt gctccctgca tttctctttt gttccttagt tggtatgata
     8221 ggtaccaaat caagttcatt atttactttt cctgtgctgc tttcgctgct cgtgcttcct
     8281 gtgctactct cactactcat gcttcctgtg ctactttcac tactcgtact ctccgtactg
     8341 ctttcgctgc tcgtgcttcc tgtgctactt tcactactcg tactctccgt actgctttcg
     8401 ctgctcgtgt tttctgtgct actttcactg ctcgtactct ctgtactgct ttcaccgctc
     8461 gtgttttctg tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact
     8521 gtccaactgc ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc
     8581 aaatcaactt tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt
     8641 gtatactcaa taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt
     8701 tctgaacttc cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc
     8761 acgcttttgt ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc
     8821 cgatcagttt tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg
     8881 attccaatca ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt
     8941 ttcttcattt ctatcactct ccatataatt accttctatt tgataaagga attatataaa
     9001 atgaaaatga tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt
     9061 cgcttgaatt tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat
     9121 atcagccttg ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt
     9181 gttttatatg catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat
     9241 atagatgctt cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga
     9301 gtggagaaaa aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt
     9361 gtccaattcc aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata
     9421 agtgcgctga aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta
     9481 ggattcaatc cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga
     9541 ccaaatctaa gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc
     9601 actgcaataa ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct
     9661 aaccgatacg cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca
     9721 acaacgagac ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct
     9781 tctattcttt ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa
     9841 tgtccgaccg taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca
     9901 agtgcattat tgacaagaat tcctaaaaca aaaccaacta agactattaa aacagatcca
     9961 atcaaccgaa taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt
    10021 ttctttcttg tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt
    10081 acgaactaag aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc
    10141 tgttagactg tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt
    10201 atgtgccttt cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg
    10261 attgcaacgt ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt
    10321 aaatcaaata atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta
    10381 tacggaaaag agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa
    10441 ctcggtccaa actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag
    10501 aaactcttta aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca
    10561 gataaagatc tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt
    10621 ctattttcga ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga aggagaaaat
    10681 ttctccaagc ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa
    10741 atgaattttg attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa
    10801 ctaaaaaacg gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa
    10861 agctattatc tagtaaaaat ggtaaaaaaa caggataaag gttctaataa agacaaatac
    10921 aaatctgagc tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga attcatggat
    10981 aaaacaatcc gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa
    11041 aatatattca aataaacaaa tttgacccaa ttggggatgt ggcaaaattt gagaagtgat
    11101 ttttggaaat tccttcttat ttttgaggca aaccactttt gccacaactt catcggggcc
    11161 tttttttatt tgtaccaaaa caaatcactg gatttgttac aatcatgagg atataattta
    11221 aaaa
//
LOCUS       DACESX010000087        11182 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.087, whole genome shotgun sequence.
ACCESSION   DACESX010000087 DACESX010000000
VERSION     DACESX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.087"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            170..1105
                     /locus_tag="IXF88_001934"
     CDS             170..1105
                     /locus_tag="IXF88_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1508658.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1195..2298
                     /locus_tag="IXF88_001935"
     CDS             1195..2298
                     /locus_tag="IXF88_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1508659.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESTLKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2311..3264
                     /gene="menA"
                     /locus_tag="IXF88_001936"
     CDS             2311..3264
                     /gene="menA"
                     /locus_tag="IXF88_001936"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1508660.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(4199..5545)
                     /gene="gor"
                     /locus_tag="IXF88_001937"
     CDS             complement(4199..5545)
                     /gene="gor"
                     /locus_tag="IXF88_001937"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1508661.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(5679..6920)
                     /locus_tag="IXF88_001938"
     CDS             complement(5679..6920)
                     /locus_tag="IXF88_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1508662.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(6938..7861)
                     /locus_tag="IXF88_001939"
     CDS             complement(6938..7861)
                     /locus_tag="IXF88_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1508663.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(8082..10574)
                     /locus_tag="IXF88_001940"
     CDS             complement(8082..10574)
                     /locus_tag="IXF88_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1508664.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(10581..11048)
                     /locus_tag="IXF88_001941"
     CDS             complement(10581..11048)
                     /locus_tag="IXF88_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1508665.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         3303 a         2277 c         1962 g         3640 t
ORIGIN      
        1 caaattttaa aataaagcgt tttttcacga aatttgctga aagaaaacgt ttgaaatatt
       61 acatgggtta tttatcaaaa atagttgact tataggctaa aaagattaga atgtataatg
      121 aatcacaaag aaaaattgtg gactagaaat ggaatggtgg ggtattaaaa tgaagaaaaa
      181 acgttttgta actggatttg ccgtattagc attttcagct ctagtgttag gcgcttgcgg
      241 cgcagatgac aacggaagct caaactcttc ttctgaatca tctacagcac aatcttcgac
      301 tgcaaaaagc acaacagaat catctgcaaa agttgtagct ggcggagatt tgcaagacgg
      361 cacatataaa ttagaagaaa aaaattattc aaatggttac cgtgcagtct ttgaaatggt
      421 tgtaaaagac ggcaaaatca ctgaatcaaa atatgataac gtcaatgaaa acggcgaatc
      481 aaaaacaaaa gatgcagaat acaacaaaaa catggaagca aaatctggca caaacccaga
      541 aaaatacatt cctgagttga atgaccaatt cttaaaagca caaagcgcaa gcggtgtgga
      601 agtcgtaact ggagctactc actcatctga aagcttccaa aactacgcac aacaattgat
      661 ccaagctgct caagctggta acacagatac aatcgaaatc gacaatggtg ctgatttgaa
      721 agacggcact tacaagcttg aagaaaagaa ctactctaac ggctaccgtg tgcaattcga
      781 aatgacagtt gctggcggca aagtaactga atcaaacttt gattatatcg acaaagacgg
      841 taaatcaaaa caagatgata cagaatataa tgaaaatatg aaagcaaaaa gcggtacaga
      901 accaaaaact tacatcccta ctctgaacga cgaattcgtc aaagcaatgg gcgaagaaga
      961 tggctcacct gctaatgtag aagttgttac aggtgctaca cacagctcac attcattcat
     1021 catgtacgca caacaattag tgaatgcagc tgaaaaaggc gacactcaaa caatcgaagt
     1081 agataatatc gtaacagaaa aataatcaca taatcatact aataaaagtt ggagggtggg
     1141 acgatagcca gattaggcag tcgtctcacc tttcaaaatg aagagaggaa aaagatgcgg
     1201 aaaaaactac tattttcctt aatcacactg ggcatctctt ttatggtgct aactggctgc
     1261 agcaactcag caaataaaac aaccactgaa agttcagcag ctaccaagat attgaaagat
     1321 ccatattctg atgaacagtt cttgttaggg acttacgttc gtatccgtgt gtatgatgaa
     1381 ggcaaagaat caacattgaa acctgccttc gatcgagtaa aagaacttgg tgataaaatc
     1441 acgatcaatc aaaaaggttc tgagatcgat gaagtgaatg agcaagctgg gatcaaacca
     1501 gtaaaagttt ctgatgacgt ctacacgtta gtcaagcggg cctacgaata tagccaagat
     1561 tctcaaggcg gattcgatat ggcgattggt gcaatcacac aattatggcg aatcggtttt
     1621 gatgacgcca gaaaaccttc ccaagaagaa atcgatcagg cattgaaatt agtggattat
     1681 cataagatcg aactaaatga caaagaaaaa actgtttatc taaaagaaaa aggaatgatc
     1741 atcgatttag gtgcaatcgc caaaggttac attacggacg aagtagtgaa agtcttgaaa
     1801 aaacaaggag tgaccactgc catcgttgac ttgggcggta atgtctatgt cttaggccac
     1861 agcccaagag gcgaaaacca agactggaca gtcggcatcc aagatccgaa catggctcgc
     1921 ggatctgttt taggttctat taaagaacgg aacaaaacat tagttacctc tggaatttac
     1981 gagcgttatt tagaagtaga tggcaaaaaa taccatcatt tgtttgatcc aaaaacgggt
     2041 tatccgttcg acaatgatat cgcaagtgtc acgatcataa cagataaatc aattgatggt
     2101 gacgggttgt ctacagcagt cttttcaatg ggtgtgaaaa aaggactgga atacgtcgaa
     2161 agcgaattga ataacgggac ggaagcgatc ttcgtgacaa aagacgataa agtatatgtg
     2221 accgatccaa tcaaagacac cttcaaatta tcggaggact cccattacac gatgggtgac
     2281 cgttctgaac taaaataagg agtacgaacc atgacactaa aaatcttttt agaagtagtt
     2341 gaaatacgaa caaaggtggc tagtgtattt ccttttgcgg taggggtatt attctctatc
     2401 gctttttttc atgagataca ttggctaaac acttttttat tttttattgg aatgctagtt
     2461 tttgatttag cgactaccgc tatcaataat tacatggatt atgaaaaggc tcattctgaa
     2521 gtatataagt atgaagaaaa tgtcattggc cgcagaaatc tatctcctgt attgattcga
     2581 aacatgattt tagctatgat tgcatttgtt tttgttatcg gcgttattct aacgatcaat
     2641 acgggttggc ttttactttt gatgggattt atctgttgtt ttattggagt attctataca
     2701 ttcggaccga ttcctttatc tcgaatgcct ttaggggaaa ttttcagtgg ggtgactatg
     2761 ggccttggaa tttttgccat ggtcgtttat gtcaatacat tcggttcaga tgtctatgat
     2821 ctgacagtca acttttcatc tggacaattt gctttggtag gaaatctttg ggctatagca
     2881 gcaatcgtat tagcttcctt acccttggtg tttacgattg ccaatatcat gttggctaat
     2941 aatttgcggg atcttgaccg tgatattgaa aatcatcgct atacactagt tttttatatc
     3001 ggacgtcctg cgggaacagt cttattccag ctattgatgt atgcttgcta tttcgtgatt
     3061 ttaatggggt tgttgagcgg tatctatgaa tggccgatct tggtcacctt cttgaccttg
     3121 ccaattgtga agaagcattt ggatcaattc aagcaaacgt taccccagcc tatcagtttc
     3181 agctattcca tcaaaaatat ggtcttattc aatgccagtt atgtattggg gctattacta
     3241 acaatcatta tgcaagccat ttaacagcat gaagaggttg tgaaaaaact gccctgcatc
     3301 aagtaataag aacagccaaa taaaaatagc tccttatatt tttcgtttgg ttgggcttat
     3361 taccgcagat gcaagctttt gaacaccgtt tatcaggagg ttgtgaaaaa gtcgcttagt
     3421 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
     3481 taataccgag aaactggctt ttgaacaccg tttatcagag gttgtgaaaa agtcgcttag
     3541 tttcgagtat taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc
     3601 ttaataccga gaaactggct tttgaacacc gtttatcaga ggttgtaaaa tcagtgtttt
     3661 gtcctgagta ttagagaaac aaatggaaaa atagttcctc atattttgcc gttttttgag
     3721 ctaataccga aggacagctg attgaacact atttgttcgg aaaaagtgac taactatgac
     3781 acaatttttt tcacagtccc ttttttcact tgatttgatt cgtaccagcg aacttgacga
     3841 gaactttctt ggtttggtaa ctatatggta ttcttttaac atcctcgttt gcttcttcta
     3901 atttatttat acaaagtcac acttttttaa acatctgttc ttcgatacat ttctatttct
     3961 tttaaacatc gataccaata ttttgaactt aagagtgcat accaaatgga ttcatttact
     4021 cttcgtcagt tacgaagata atcaaaatct ccaacttccc tacttatttc gttttgagaa
     4081 ttatcgaagc aatcttacat aaataactaa aaaacaaaaa accagaaaat cagtatactg
     4141 attttctggt tttacatggt ttgttcaata aaaagctgtt ggaactgtta atattaaatt
     4201 atcgcatcgt tacgaattct tccgctcctg ttggatggat ggctactgta ttgtcaaagt
     4261 cttctttagt tgcacccatc ttgatagcca cggcaaaacc ttgcagcatc tcatcgacac
     4321 caacaccaat agcatgtaag ccgacgattc tttcttcttt tccgacacag atcaatttca
     4381 tttcgcattt ttggcgataa tcatttaatg caaagtacat tggggtaaag cgtgaacgat
     4441 aaactttcaa ttcattttcg ccatattttt ccaatgcttc ttcttctgtc aaaccaattg
     4501 ttgcaattgg tggatgcgta aagacgactg tcggaacaag tttatagtca agataagaat
     4561 ctttctttcc attaaataaa cgttctgata aacgtctacc agctgcgatt gcaacaggag
     4621 tcagatctaa tttaccgatc acatcaccaa cagcaaaaat atgttcatcc gttgtatttt
     4681 ggtatttatc tactttgata aacccttttt tgtccaattc cacatttgta ttttccagac
     4741 ctaatttttc ggtattcggt acacgtccac cggcaaaaag tacacaatca ccagttaagg
     4801 tttcaccatt ttcaaatagc accgtatatt cgtcattgtt tttagtaatt tctttagcaa
     4861 tatgatgtgc gtaagttttt attccagctt cttggtacat ttggatcaaa ttgtccgaaa
     4921 gcattttatc aaacgttcta agtggtcgtt cttttcgata tgcccacatg acttcactac
     4981 ctaaaccacg aaatacacct gaaagttcag ccgcaatgta tccagcacct aaaacgattg
     5041 ctctcttagg taactctgtt aattcaaaaa atccatttga atcaattgcg tattctccac
     5101 caggaatggt cattttactt gcacggcctc cagtagcgat caagatttta ggagcacgat
     5161 aggtttcacc gtttacttcg atcgtttgac tatcgataaa agtagcgtaa ccttttatag
     5221 cctcgacatg attgctatct aatcctcttt gataagcacc gtgtagaaaa tcaatatatt
     5281 tttctcgtct ttctactaga ccggcgaaat caacattttt taaatctgct tggataccgt
     5341 aacttgaggc atctcgttca attgtttcca aaatcgtact tgcttgccac ataacttttt
     5401 taggtacaca accgacgttg acacatgttc cgcccaattc attcgcttca atcagcaaaa
     5461 cttttgcgcc atgcatccct gctctgtttg ctgaagcaat accgccagag ccgccaccaa
     5521 ttacgatgta atcatatatt tgcattttgt tcctccttat tgaagagatc aatcaaaacc
     5581 tttatttaac gatccttttt ctacagaaaa caagtgtaac acttttcttg aattttgtat
     5641 gcgaagatgc ttaaaacagg gatatatcag ctttttcctt atttacgttt tcggatcata
     5701 tctccaggtt tcaccggctg tgcgactggc atagttaaaa tcatcattgg atttggtgct
     5761 cggtcaatgg attcgccttc ttcattatgc ataacttcaa cggtttgact gaaatgactg
     5821 aatcctggac cataaaattc gatttcatct cctacagaaa acaagtttct ctgacgtatc
     5881 gtagcaatct gtgttgcctc atcataagat aatacttcac cgacaaattt atatactggg
     5941 attttacgac gttcaccgaa taattgttcg ttttcagttg gtgtattgta gtaaaaacct
     6001 gtagctaatt ctcgttgtgc tactttccat aactcgtcga tccattcttg cttgcagaca
     6061 tagttttcag gatcttccat ataactgtct acagccgctt tatagacatt tgacaccgta
     6121 gatacataat ggatcgattt cattcttcct tcgatcttga agctgtctac gccgttttca
     6181 atcaattctg gaatatgttc gatcattgac atatccactg cactcatcga gaattcttct
     6241 tccacttcgc ctgtatcagt taaactgttt cgttcttgac caaaaggcat atcgaacaat
     6301 ccatacttcc agcgacaaga ttgagaacat ccgccacggt ttgcatcccg catcgacata
     6361 tggttggaca atgtgcatcg accagaataa gagatacaca tggcaccatg gataaatgct
     6421 tcgatttcgg tatccgtatt tttacgaatc tctgccactt cctccattga aacttcacga
     6481 gctaaaacga cacgttccaa cccttcttct ttccagaatt ccaatgtctc aaaattagtt
     6541 gctgaagctt gagtggataa atggatcggc agacctgggg cttctgtcgc acaaatttcg
     6601 atcaaagctg gatcagaaac aatcactgca gaaataccga catcacgcaa actgcggaaa
     6661 aattcccctg ctccatcttc atttccttca tgggtgacca tattagcagc aacgtatacc
     6721 ttcgcatcat gttcttttgc aaaagcaact ccttcagcca tttcttcata agtaaaatta
     6781 cctgcacggc ttcttaaacc atatgcattc ccgccaatat atactgcatc tgctccataa
     6841 tggatcgcgg tttttaactt ttccagtgtt cctgctggcg ctaggatttc tggtcgtttc
     6901 aattttcttt ttactgtcat ttctttcatc atactcctta tttgatagca gaaggatcaa
     6961 tataaaagaa tcctgtatct aattcacggt tttcaggatg caatttactg atttcgctag
     7021 tcattttttc cgctttttct tgatcccagc taccttctaa aatttgtttt tttgcttcgg
     7081 cgaatagtcg agtgatgttt acgaattttt cgcctggagt aaataatccg tccaatttcc
     7141 atgtacagta atgatgctga taaagttcat tcaattcatt cgagagattc agatcattgc
     7201 tggcaaaaat atgtgtgcca tgactatctt cataaataga ataatgtgtc tcttcttttt
     7261 taggttccga gataaaaaga cctctttcac gatctttttg ttcgtcttgt ttcgtatagt
     7321 tatagtaatt ctgtagcagt ggacgtttgg attgatggat gcatgtagca ccatacacaa
     7381 gggtttctac aggaatatcg agtttttctt ccatcgcctt catctcttca aaaggaactt
     7441 cacgagcaag tactgctcca actgcgcctt ttttcgccca gaaattgatt tgacgtgcgc
     7501 tcgttactaa tgtttcagca tcataaatat acggtatacg caattcagga ttttttttca
     7561 tcgtataaat aatacctgta tcaccaactg tgatcacatc tacgtttatt tcttttaaaa
     7621 aagccaaata ttcaggtaca tttttcattt tttcaggatg aatgatccca tttacagcaa
     7681 tattcgcttt tttaccatac ttgtgtgcta attctaccaa ttgtcgctgt tcttctcgtg
     7741 taaatgaagc aggcagacgc aatccaaatt gctcttcacc gaaatatagt gtatctgcac
     7801 ctgcagcaag caactgttct gcttgttcaa tagtttctac tgtggttatg agttcgatca
     7861 taattttctc ctcctacaac ttcataatag tagcatagct ctaaaaactt tccaatactt
     7921 cttctattta aagtcggatt tttgaaaatt atttcacaaa aaatcaaaat aaagttcgat
     7981 atattctgtc caaatataaa aagagattgt gacaaaaatc ttgtcacaac ctcttaatca
     8041 agtcttacta ttataaaaat tcagctacac aggccaagta actataggga acctactgtt
     8101 tcctttttag gtccgcgaac attcaatgtg atcttacctt ttgaagcacc tactgttacg
     8161 tggtcaccta aacgaatttc tccgccaagc aaagcctcgc tcaatcgatc ttcaatttct
     8221 ttttgaagtg cgcgacggat tggacgtgca ccatattcag gatcaaatcc agctttcccg
     8281 atcacatcga ttgcagatgg tgtgattttc acttgaatat cttgttcttt caatcgtttg
     8341 ataatcgctt tgctcatgat ttggacgatt gtatggattt catctttact caatgaatgg
     8401 aagacgacag tctcatcgat tcgattcaag aattcaggtc ggaaagcttt ctttaactct
     8461 tctaagatac gtttttgcat ggctttgtga tctttagtaa catcttggac attgaatccg
     8521 acattttttt cttctcgaat ttgtgtcgct ccgatatttg aagtcataat catgattgta
     8581 tttctaaagt ccacttttct acctttagaa tctgttaaat gaccatcatc caatacttgc
     8641 agaagtaaat tgaaaacgtc tggatgtgct ttctccactt catctaagag aatcactgaa
     8701 tatggttttt gacgaatttt ttctgtcaac tgtccacctt catcataccc tacgtatcct
     8761 ggaggagacc cgattaatcg gcttgtactg tatttttcca taaattctga catatcaaca
     8821 cggatcaggg catcttcact tccaaacatg acttctgaaa gtgctttggc caattctgtt
     8881 tttccgacac cagttggtcc aaggaacatg aatgaaccaa ttggacgatc aggatctttc
     8941 aatccgcttc gtgcacggcg gatcgcacgt gaaactgctt tgacagcctc atcttgacca
     9001 acgacacgtt catgcagaag tccttccagt tctaacaagc gttcgctttc ttttttctcc
     9061 aactgctgta gagggacacc agtccactcc gatacaactg tagcaacatc ttcttctgtt
     9121 acacgatcag aataaccttg ctcctgtttt gcttcaattg cagaaacttt ttgcagcttt
     9181 ttacgtagtg ctttttcttg tctgcgtaat tgagcagcat tttcaaagtc ttgtttttgg
     9241 atcgctgctt ccttttcttg tactagttca tcgatttcta gtttgatcac agttgatttt
     9301 gttaagtgat cggtttgatc caaacggact ttcgctgctg attcatcgat caaatcaatt
     9361 gctttatcgg gtagttgacg agaagtgata taacgaacag aaagattgac tgctgctcgt
     9421 acagcttcat ctgtgatctc cactccatgg tgctcttcat atcttgtacg taatccttga
     9481 agaatcactt ctgcttcttc aggagttggc tcatctactt gaatgcgagc aaaacgtcgc
     9541 tctaatgcag aatctttttc gatatatttt tgatattcat ctagggtcgt tgctccgatc
     9601 gtttgtaatt ctcctcgagc taacgccggt ttcaagatat tcgaagcatc aattgctcct
     9661 tctgctccac ctgcaccaat taaggtatgc aattcatcaa tgaataagat tacttgacca
     9721 tcattataga tttcgtcgat gacctttttc attcgatctt caaattcacc gcgatatttc
     9781 gtaccagcaa ccaaagatcc catatcaagc atcatcaggc gcttttcttg catgtcttct
     9841 ggcacttcac ctaaaatgat tttttgagct aacccttcag caatggctgt tttgcctaca
     9901 ccaggttcac ccaccagtac agggttattc tttgtccgtc ggctaagaat ctggataagc
     9961 cgtttcactt ctctcgaacg accaacaact gggtccagct tgttttcttt tgctaatttc
    10021 gtcaaatcac gtgctaatga atctagcgta ggtgttcctt gcggtgtttg ccgtttatct
    10081 tgtacattta catttctacg acgattcatt ccttttccag cagaatcacc agctagcccc
    10141 attttttgtt tcagaacttg acgggctttt gaaaggctga tacctaagtt gatcatgatt
    10201 cgagtagcca tgatactttc atcttttaat aagctcatca aaatatgttc tgttccaact
    10261 ttttgagaac cagttttctt cacttcgctg ctagctaaag caaaaacttg tttcattctt
    10321 ggtgaatatg gaagaaagac tcctttaggg taagaacgga taccaccata accaatcaga
    10381 tgttcaattt cttcataaat ttccgtttca ttcaaatcca tttcacgtaa tacttttcca
    10441 gcaattccat tttgctcgac cactaaggct agtaaaacat gttctgaact aactgattga
    10501 tgtttcaacc gttttgcttc ttcttgtgca atttctaata ctgcttgggc tctttcagta
    10561 aaaagttctt ccatctaatc tcacttccca tcttcataac ttaaacgttc cagaaatgct
    10621 cggagaatcc gagcacgtgt atgatcttca tattcattga aattcaatgt gttttttgca
    10681 attgcactca acatcaagtt tccttctttt tttgtaatta tttggtcttc ataaagtttt
    10741 tgaataatag caaaagcatt tttctcactg attgtatcat caaataactg atttatctgc
    10801 tcaagtaatt gttttttatc tgaaatcctt acttttgcaa ttcgtatata accaccacca
    10861 ccacgtttgc tttctactag ataaccacgt tggatggtaa aacgggtatt aatcacatag
    10921 ttgatttgtg aaggaacaca gttaaagagg tctgccattt caatccttcg aatctcgatc
    10981 atttcactat tttccagaat tttctttata tatgattcaa ttaaatccga ggtattctga
    11041 tgtgccatct tattcattcc ctttgactaa tattgacctt aattataacg aatttgataa
    11101 tagaaagaaa gaagaacgct ttttgttaaa taaatccttt agaatcaaca aaaaaaaaat
    11161 acggaatata ctattccgta tc
//
LOCUS       DACESX010000088        10825 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.088, whole genome shotgun sequence.
ACCESSION   DACESX010000088 DACESX010000000
VERSION     DACESX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.088"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            5..2377
                     /locus_tag="IXF88_001942"
     CDS             5..2377
                     /locus_tag="IXF88_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508666.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="IXF88_001943"
     CDS             2438..3898
                     /locus_tag="IXF88_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1508667.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="IXF88_001944"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="IXF88_001944"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1508668.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6353..6946
                     /locus_tag="IXF88_001945"
     CDS             6353..6946
                     /locus_tag="IXF88_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ1508669.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="IXF88_001946"
     CDS             6959..7858
                     /locus_tag="IXF88_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1508670.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="IXF88_001947"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="IXF88_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1508671.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATERRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="IXF88_001948"
     CDS             8724..8984
                     /locus_tag="IXF88_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508672.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="IXF88_001949"
     CDS             9429..9635
                     /locus_tag="IXF88_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1508673.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9683..9886
                     /locus_tag="IXF88_001950"
     CDS             9683..9886
                     /locus_tag="IXF88_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508674.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="IXF88_001951"
     CDS             9902..10339
                     /locus_tag="IXF88_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508675.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..>10825
                     /locus_tag="IXF88_001952"
     CDS             10332..>10825
                     /locus_tag="IXF88_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508676.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTES"
BASE COUNT         4088 a         1804 c         2270 g         2663 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agacgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatc
//
LOCUS       DACESX010000089        10431 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.089, whole genome shotgun sequence.
ACCESSION   DACESX010000089 DACESX010000000
VERSION     DACESX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.089"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(166..651)
                     /locus_tag="IXF88_001953"
     CDS             complement(166..651)
                     /locus_tag="IXF88_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1508677.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1131..1772
                     /locus_tag="IXF88_001954"
     CDS             1131..1772
                     /locus_tag="IXF88_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1508678.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1791..2426
                     /locus_tag="IXF88_001955"
     CDS             1791..2426
                     /locus_tag="IXF88_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508679.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2444..3259
                     /locus_tag="IXF88_001956"
     CDS             2444..3259
                     /locus_tag="IXF88_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1508680.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3578..5014
                     /locus_tag="IXF88_001957"
     CDS             3578..5014
                     /locus_tag="IXF88_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1508681.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5203..7650
                     /locus_tag="IXF88_001958"
     CDS             5203..7650
                     /locus_tag="IXF88_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1508682.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTELEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7880..8947
                     /gene="rlmN"
                     /locus_tag="IXF88_001959"
     CDS             7880..8947
                     /gene="rlmN"
                     /locus_tag="IXF88_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1508683.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9140..10243)
                     /locus_tag="IXF88_001960"
     CDS             complement(9140..10243)
                     /locus_tag="IXF88_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1508684.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3446 a         1787 c         2275 g         2923 t
ORIGIN      
        1 atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca
       61 tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata
      121 aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata
      181 aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa
      241 cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc
      301 gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc
      361 tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc
      421 ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag
      481 atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag
      541 ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat
      601 acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca
      661 ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta
      721 aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt
      781 agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg
      841 attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta
      901 gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg
      961 attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac
     1021 tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc
     1081 ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt
     1141 tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat
     1201 ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt
     1261 ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt
     1321 tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt
     1381 tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc
     1441 gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa
     1501 gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt
     1561 ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt
     1621 taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt
     1681 tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc gtaatgaaac
     1741 aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat
     1801 tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca
     1861 ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt
     1921 tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac
     1981 cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact
     2041 tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat
     2101 tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga
     2161 ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag
     2221 cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg
     2281 ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg
     2341 gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat
     2401 tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt
     2461 tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc
     2521 agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac
     2581 atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg atattgattt
     2641 agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc
     2701 aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa
     2761 aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt
     2821 gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt
     2881 aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa
     2941 agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga
     3001 tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt
     3061 gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga
     3121 ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct
     3181 agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga
     3241 tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt
     3301 ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc
     3361 acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc aaatttatgc
     3421 aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt
     3481 tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa
     3541 gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag aacttacctc
     3601 atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag aaggagcttg
     3661 gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac ctggaaagac
     3721 atttaaagga acgaacggtg atttggcagt agaccactat catcgttata aagaagatat
     3781 tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt ggacacggat
     3841 ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg agagattgat
     3901 cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt gggatcttcc
     3961 acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg aagatttcac
     4021 tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact ggattagttt
     4081 gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc cgccaggtgt
     4141 gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa atgcatctgt
     4201 catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac
     4261 gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa atgcagaaga
     4321 cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga ttgctgccat
     4381 gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg cagaattgct
     4441 agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga cgaatgcatt
     4501 caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc
     4561 agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg tcgaacgaac
     4621 aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc gtatcacaag
     4681 ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg acaagttgac
     4741 agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc acgtcaaagc
     4801 catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct ggtcttatac
     4861 agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat atgttgatca
     4921 agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt actggtacca
     4981 aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac
     5041 ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa
     5101 aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac
     5161 ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg
     5221 cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa
     5281 atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa
     5341 ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac
     5401 caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag
     5461 ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta
     5521 ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg
     5581 acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt
     5641 ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga
     5701 agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa
     5761 ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa
     5821 ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc
     5881 gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca
     5941 gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag
     6001 gaaccagaaa atacagagct ggagcagcta agttttgttc caatcgacag ttttcaagaa
     6061 aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca
     6121 gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt
     6181 gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata
     6241 ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa
     6301 caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc
     6361 gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta
     6421 acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca
     6481 ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga
     6541 gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt
     6601 gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct
     6661 ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg
     6721 cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat
     6781 atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg
     6841 caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc
     6901 ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga
     6961 acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg
     7021 atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca
     7081 gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc
     7141 atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg
     7201 acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg
     7261 ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta
     7321 gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg
     7381 ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa
     7441 gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga
     7501 tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt
     7561 gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca
     7621 gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa
     7681 taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt
     7741 ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat
     7801 ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga
     7861 gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt
     7921 aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg
     7981 gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat
     8041 cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc
     8101 ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt
     8161 actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat
     8221 cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga
     8281 aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg
     8341 cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat
     8401 ggactttttg catatcatca atgatgccaa aggattggca attggtgctc ggcacattac
     8461 agtttccact agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt
     8521 caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat
     8581 caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac
     8641 aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca
     8701 tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat
     8761 tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt
     8821 gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg
     8881 tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc
     8941 caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta
     9001 aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt
     9061 tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga
     9121 tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag
     9181 catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag
     9241 gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg
     9301 tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat
     9361 agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac
     9421 tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga
     9481 atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag
     9541 taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat
     9601 ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga
     9661 ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt
     9721 ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa
     9781 ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta
     9841 cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag
     9901 tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag
     9961 cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca
    10021 actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta
    10081 tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc
    10141 caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat
    10201 ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc
    10261 ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag
    10321 tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca
    10381 tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa t
//
LOCUS       DACESX010000090        10268 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.090, whole genome shotgun sequence.
ACCESSION   DACESX010000090 DACESX010000000
VERSION     DACESX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.090"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            96..602
                     /locus_tag="IXF88_001961"
     CDS             96..602
                     /locus_tag="IXF88_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1508685.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            619..1518
                     /locus_tag="IXF88_001962"
     CDS             619..1518
                     /locus_tag="IXF88_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1508686.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1605..2489
                     /locus_tag="IXF88_001963"
     CDS             1605..2489
                     /locus_tag="IXF88_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1508687.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2681..2752)
                     /locus_tag="IXF88_001964"
     tRNA            complement(2681..2752)
                     /locus_tag="IXF88_001964"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2717..2719),aa:Arg,seq:ccg)
     gene            2812..4146
                     /gene="rpoN"
                     /locus_tag="IXF88_001965"
     CDS             2812..4146
                     /gene="rpoN"
                     /locus_tag="IXF88_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1508688.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4349..5386
                     /locus_tag="IXF88_001966"
     CDS             4349..5386
                     /locus_tag="IXF88_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1508689.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5427..6428
                     /gene="gap"
                     /locus_tag="IXF88_001967"
     CDS             5427..6428
                     /gene="gap"
                     /locus_tag="IXF88_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1508690.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6538..7728
                     /locus_tag="IXF88_001968"
     CDS             6538..7728
                     /locus_tag="IXF88_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1508691.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7832..8587
                     /locus_tag="IXF88_001969"
     CDS             7832..8587
                     /locus_tag="IXF88_001969"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1508692.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8703..10001
                     /gene="eno"
                     /locus_tag="IXF88_001970"
     CDS             8703..10001
                     /gene="eno"
                     /locus_tag="IXF88_001970"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1508693.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3606 a         1893 c         2129 g         2640 t
ORIGIN      
        1 tataaaaatc ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg
       61 tacgtcatat taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa
      121 aacggcagat cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa
      181 aaatgttccg tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc
      241 aagatagttt tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta
      301 gtgcatggtt ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa
      361 ggacgcctgt ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt
      421 ccgttgcaga gctccatatg attttagata aaatagacag tccggaaaaa gagattctga
      481 tcctctactt ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg
      541 aaaacacagt taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat
      601 gaaggagaga agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact
      661 tataaagaac aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc
      721 gaagagcaat catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta
      781 tctttggcta tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc
      841 tttgcagaag atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt
      901 actgatcgat ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa
      961 gaaaataaaa caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt
     1021 gaaaaagctg aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat
     1081 tatcagaaaa tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca
     1141 gcagcagata gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag
     1201 ctctcacttc cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc
     1261 aaacggcaga tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa
     1321 gatattcaag aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat
     1381 ttgaacaaag agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat
     1441 atgggtacac cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa
     1501 gattatttgg atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag
     1561 tacaagtaaa taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt
     1621 tagttatcag tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct
     1681 ccttgatcac cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca
     1741 aacaatcaga acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag
     1801 ccattcaaga atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta
     1861 caaaaacgag ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag
     1921 gagcgaccac aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt
     1981 ttttcgtctt aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag
     2041 cagggcacga gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc
     2101 agtcgatgac ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa
     2161 cccccacttt tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc
     2221 agcgaccgat catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt
     2281 tgattttgca gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata
     2341 tctatcctga aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt
     2401 atcgcatcac gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg
     2461 gcagaaacga tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat
     2521 tttaataagt agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca
     2581 ggtacaacaa aaaactgtcc accaattttc tttggcggac agttttcact taaaaataaa
     2641 gaagcaacta ttcgtaaatt atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa
     2701 cccccgtctc aagaaccgga atcttacgtg atatccacta cactaaggac gcaagcactg
     2761 ggataattgt aactaaacag ataaaaaatt acaaggggat tggacaaaaa aatgaaattt
     2821 gagcaaaatt tttcacaaaa acagcagcag acacagaaac tagccatgac gcaacagttg
     2881 caacagtcca tccaagtttt acaatataac agtgaggagc tgttggcatt cgttgagaat
     2941 caagcaatgg aaaatccatt ggtggaagtt gttgagccgg aatggcagcc agattacata
     3001 aaagcttctt catcttctta tgaaggagaa gagacaaatt acctaaatca gattcctgat
     3061 acaaaagggt ctttatttga ttcattgatc gaacaagtcc atttgaatta ccgagatact
     3121 tttttgagga aaatcgttct ttatcttgta gaatacatcg atttgaacgg atttttgacg
     3181 attagtttag aagaagccgg aaaagagatt ggagctacgc ctatccaaat gctcgatgcg
     3241 ttgactctga ttcagcaatt agaaccagct ggagtaggtg cacgtagttt gcaagaatgt
     3301 ctcatgcttc aaacagaacg agatgattat gcgccggaat tagcttatat cgtgttggaa
     3361 gaatgttttg aggaactggt ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt
     3421 gatctacatg ctgttcagca gatattcgac tatttacaaa cgttgagtcc aagccctggc
     3481 cggattttcg accgtagcag cgaattgtac attattcctg atgtccgggt attagtggac
     3541 gacgataaaa atgtacaagt tatctcaaac cgaaaaaatc agccaaatat acgctttcaa
     3601 gagagttact tcaaacaaat gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa
     3661 gaacggaaac aggaattcga atggttgaag aaaacgatcc tacaacgtgg ggatacaatt
     3721 ttgcgggttg ctcaagtcat tgtttcccgg caaaaagctt tctttatcga caaggaacgt
     3781 ccaatcaagc ctcttacgtt aaaggaagtt gcaactgaga tagatgtgca tgaatccacg
     3841 gtcagtcgtg cagtcaacgg gaaatatttg gaaacagact tcggtgtatt tgaattaaaa
     3901 aaatttttca ctacacgaat tcctacgaat agtacagaac agacagaaga cctttcagca
     3961 gatacagcga agaaaaagct gcaggaatta gtcgatcaag aagacaaaaa caagccgttg
     4021 tctgaccaga aattggttga attgctgaaa aaagatgaga tagcgatctc aagacgaaca
     4081 gtagcaaaat atcgtgattt attaggaatc ccgagttcat caaaaagaaa acgttatgat
     4141 aactaatcag tcaaaagact agaaaaagta tttcactttt tctagtcttt ttagttgaaa
     4201 aacgagagca aaactgttag acttacagtc gtgaggttga gatttccctt tattgatgtg
     4261 aaattcgaat agttaatttc tttttttatc gtatatgggt cgttttaagt ctacattgga
     4321 cgtaaagtgt ccaactaagg agatcgctat gctggatgaa ataaaaatga ttgaatctgt
     4381 tgcaccagat attatcgaag tgttgcaaga acgctataaa attttacgaa atatctattg
     4441 gatgcagccg attgggcgtc gaagtttatc ggaaagtatg gggatcacag aacgagtctt
     4501 acgaacagaa acagaccttt tgaaaaatct gaatttgatc gacacctcaa aaagcggtat
     4561 gacgctgaca actaagggcg aagaagttta tcaaagcctt gaaaacttca tggatcagct
     4621 tttaggtacg catcaaaccg aacaacagtt ggcagaatat tttggtaacc aacgctgtat
     4681 cgttgtttca ggaaacagcg aagagcagac caaagtggct gatgcattcg gcgaagcgct
     4741 gagcgaagcc ttagaccgac tacttccaga aggcgagaat atcatcgctg tgatgggagg
     4801 aactactatg gctgtggtag ccgaacaatt gtctaatctg gaaaataaga aacggcataa
     4861 cctgtttgtc cctgcaagag gcgggattgg tgaagcgata actgtacagg caaattctgt
     4921 cagtgcaaga atggctgcta aagcaaacgg taaccaccgc gcactttatg ttccggaaca
     4981 attaagtctg gcaacttaca attctttact caatgagcca tctatccaag aagtcttaaa
     5041 cttgattagt gaagcgaatt gtgttattca tagtattggg cgtgcattac acatggcagc
     5101 acgtcgtaag atgactgaaa aagaattagt catgctgaaa caagcaaatg ctgttgcgga
     5161 atcttttggc tactttttca atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg
     5221 catagaattg caagatttgg aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc
     5281 aaaagctaaa gcaattcgag cttatatgaa aaatgcacca aaacaaacgt ggctcatcac
     5341 tgatgaagct gctgcaaatg agattttaaa aggggtaacc ctttaaaata aatatttttt
     5401 tgattttcat aaggaggaaa tcttaaatga cagttaaagt aggtattaat ggttttggac
     5461 gtattggacg tctagcattc cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa
     5521 tcaacgactt gacagatgct aaaatgttgg cacacttgtt aaaatacgac acaactcaag
     5581 gacgtttcaa cggaacagtt gaagttcatg aaggttcatt caacgtaaac ggaaaagaag
     5641 ttaaagtatt agctaaccgc aacccagaag aattaccatg gggcgaacta ggcgtagata
     5701 tcgttctaga atgtactggt ttcttcactt ctaaatcagc agctgaaaaa catttaacag
     5761 ctggtgctaa acgcgttgtt atctctgctc ctggcggaaa cgatgttcca acaatcgttt
     5821 ataacacaaa ccacgaaaca ttaactggta aagaaacagt tatctcaggt gcttcatgta
     5881 ctactaactg tttagctcct atggctaaaa cattgaacga caaatttggt gttgttgaag
     5941 gattaatgac aactatccac gcttacacag gtgaccaaat gactctagac ggacctcatc
     6001 ctaaaggtga cttccgccgt gcacgcgctg ctgctgcaaa catcgttcct aactcaacag
     6061 gtgctgctaa agctatcggt ttagtaattc ccgaattgaa cggtaaatta gacggagctg
     6121 ctcaacgtgt tcctgtacca acaggttcat taacagaatt agtaacagtt cttgaaaaag
     6181 aagtaactgt tgacgaaatc aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat
     6241 acaacacaga cgaaatcgtt tcttcagata tcgttggtat gacttacggt tcattatttg
     6301 atgctacaca aactaaagta atgacagttg gcgacaaaca attagttaaa actgttgctt
     6361 ggtacgacaa cgaaatgtca tacactgcac aattagtacg tactttagaa tacttcgcta
     6421 acttataaga tagacaaacg tacaagtgta aattatagac atgtacattt aaaagcgggg
     6481 aagcaatcgc gcttcttccg ctttttttga taataagttt attcaggagg aaataaaatg
     6541 gctaagaaaa cagtgaaaga tatcgattta aaagacaaaa aagttcttgt ccgtgttgac
     6601 ttcaacgttc ctttgaaaga cggcgtgatc actgacgaca ctcgtatcaa agcagcattg
     6661 ccaacaatca actacgttct agaacaaggc ggaaaagcaa tccttttctc tcaccttgga
     6721 cgtgtaaaaa cagaagaaga taaagaagga aaatctttag ctccagttgc aaaacgtcta
     6781 ggcgaattat taggtaaaga agtaacattc gtacctgaaa ctcgcggaga acaattagaa
     6841 gaagcaatcc gcaacatgaa tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa
     6901 gatattgacg gtaaaaaaga aagcaaaaat gatacagaac taggtaaata ctgggcttca
     6961 ttaggcgatg tatttgttaa cgatgctttc ggtacagctc accgtgcaca tgcttctaac
     7021 gtaggtatcg cttcaactgg tatcccaaca gttgctggat tcttgatgga aaaagaaatc
     7081 aagttcatcg gtgaagcagt agaagaacca aaacgaccaa tgattgcaat ccttggtggc
     7141 gcaaaagttt ctgataaaat cggcgtaatc gaaaacttga ttcctaaagc agataaaatc
     7201 ttgatcggtg gcggaatgac ttacacattc tacgaagcaa aaggaatcaa gatcggtaac
     7261 tctctagtag aagctgataa agtggaacta gcaaaagaat taatcgaaaa agcaggcgac
     7321 aaacttgtct tgccaatcga caatgtctgt gcaccagaat tttcaaatga tgttgaaaca
     7381 caagtaatcg aaggagacat tcctgatggt ttgatggctt tagacatcgg cccagcatca
     7441 gtaaaattat ttgctgatac attacaaggt gcaaaaacag ttgtttggaa cggacctatg
     7501 ggtgtatttg aaatgagtaa ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct
     7561 aacttagaag gcgcaacaac aatcatcggt ggcggagact cagccgcagc agcagaacaa
     7621 ttaggatttg ctgacaaatt cacacacatc tcaacaggtg gcggcgcaag cttggaattg
     7681 cttgaaggta aagaattacc aggactagca gcaatcaacg ataaataaac gaccaagttt
     7741 gctcaattct gacgagtaat cgaacaaaag gtgaagggag ttttcatgac tccctagcca
     7801 tttttcaata aattaaggag tgtgttcctc aatgcgtaaa cctattatcg caggtaactg
     7861 gaaaatgaac aaaactgcat cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat
     7921 cccttcaaat gacaaagttg attcagtcat cggatctcct gcattattct tacaagaatt
     7981 aatgacatca gctgaaggta cagaactaaa aatctcagca caaaactgct actgggaaaa
     8041 ctcaggtgcg ttcactggtg aaacttcacc agcagctctt gctgatctag gtgttcatta
     8101 tgtgatcatc ggacattcag aacgtcgtga atatttccat gaaacagacg aagacatcaa
     8161 taaaaaagcg aaagctatct ttgcaaatgg tatgacacca atcctatgct gcggcgaatc
     8221 tttagaaacc tatgaagcag gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg
     8281 tttagctgat ctttcagctg aacaggtgtc aaacatggtt attgcttatg aaccaatctg
     8341 ggcaatcggt actggtaaat cagctgacgc acaaatcgct gacgaaattt gtggagttgt
     8401 acgtcaaaca gttgaaaaac tttatggaaa agaagtatca gaagcagttc gtatccaata
     8461 cggcggttct gtaaaaccag aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg
     8521 tgctttagtc ggtggcgcaa gcttgcaagc agattcattc ttgtcattgt tagacgctgt
     8581 aaaataattg ttgaaaattt gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt
     8641 aactaaaatc gtatatgagg gaaatccctt aggaaaaaac aaactcaaag gagagacaaa
     8701 acatgtcaat tattactgat gtttacgctc gcgagatctt agactcacgt ggtaacccaa
     8761 caatcgaagt agaagtatac acagaaagcg gagcttttgg ccgcggaatg gttccttcag
     8821 gtgcttcaac tggtgaatac gaagcagttg aattacgtga cggagacaaa gctcgttatg
     8881 gcggtaaagg tgtaactaaa gctgtcgaca acgtaaacaa catcattgct gaagcaatca
     8941 ttggctacga tgttcgtgac caaatggcta tcgacaaagc tatgatcgct ttagacggaa
     9001 ctcctaacaa aggtaaatta ggtgcgaacg ctattttagg tgtttctatc gctgttgcac
     9061 gtgcagctgc tgactaccta gaagtacctt tataccacta cctaggcgga ttcaacacaa
     9121 aagtattgcc aacaccaatg atgaacatca tcaacggcgg atcacatgct gataactcaa
     9181 tcgacttcca agaattcatg atcatgcctg ttggcgctcc aacattcaaa gaagctctac
     9241 gtatgggtgc tgaagtattc cacgcattag cttcaatctt gaaagctcgc ggattagcaa
     9301 cttctgttgg tgacgaaggt ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg
     9361 aagtaatcat cgaagcaatc gaaaaagctg gctatgtacc tggtaaagac gttgttcttg
     9421 ctatggatgc tgcttcttca gaattctacg acaaagaaaa aggtgtttac gtactagctg
     9481 actcaggcga aggcgaaaaa acaactgacg aaatgatcaa attctacgaa gaattagttt
     9541 ctaaatatcc aatcatctca atcgaagatg gattagacga aaacgactgg gacggattca
     9601 agaaattaac tgacgtatta ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa
     9661 caaacactca aaaattatct gaaggtatcg aaaaaggaat tgctaactca atccttatca
     9721 aagtgaacca aatcggtaca ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag
     9781 ctggctacac agcagttgta tctcaccgtt ctggtgaaac agaagattca acaatctctg
     9841 atatcgctgt tgcaacaaac gctggccaaa tcaaaactgg ttctctatca cgtactgacc
     9901 gtattgctaa atacaaccaa ttactacgta tcgaagacca acttggtgaa gttgcagaat
     9961 acaaaggttt gaaatctttc tacaacttaa aaaacaaata attcttttct aatgaagata
    10021 catgatcgac tattgaaaga tagtcgagta tgatcactta gttaagtaat cgaactctct
    10081 acttgctgaa atatcagcag gtagagagtt tttttgcaac tagcttctgt atatgatgtt
    10141 tacaaagaat ggtacaagaa aagatcatcc ttgaaaaagg aaagctaaca ataactagca
    10201 tcatataaga gcttttaagg atagaacagt agaataggaa tctatgggca agtcctctcg
    10261 tataaata
//
LOCUS       DACESX010000091        10175 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.091, whole genome shotgun sequence.
ACCESSION   DACESX010000091 DACESX010000000
VERSION     DACESX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.091"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..1059
                     /locus_tag="IXF88_001971"
     CDS             <1..1059
                     /locus_tag="IXF88_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1508694.1"
                     /translation="LQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYRE
                     YLEGQSLVGIGRALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLT
                     KKRVKNEGHVPQYYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYAL
                     SAITFCGDCGDIYRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTA
                     INKLLAGGNNMIKTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHL
                     ADEIDELRDKRQILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVT
                     VYEGHFVIAFKSGIEMEI"
     gene            1202..1426
                     /locus_tag="IXF88_001972"
     CDS             1202..1426
                     /locus_tag="IXF88_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1508695.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            1441..2310
                     /locus_tag="IXF88_001973"
     CDS             1441..2310
                     /locus_tag="IXF88_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1508696.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2291..3025
                     /locus_tag="IXF88_001974"
     CDS             2291..3025
                     /locus_tag="IXF88_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1508697.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGVADEMRRPMMLIVSAKKKM"
     gene            3058..3966
                     /locus_tag="IXF88_001975"
     CDS             3058..3966
                     /locus_tag="IXF88_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ1508698.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3963..4505
                     /gene="sat4"
                     /locus_tag="IXF88_001976"
     CDS             3963..4505
                     /gene="sat4"
                     /locus_tag="IXF88_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1508699.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4598..5392
                     /locus_tag="IXF88_001977"
                     /note="aph(3')-IIIa"
     CDS             4598..5392
                     /locus_tag="IXF88_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1508700.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5500..5688)
                     /locus_tag="IXF88_001978"
                     /pseudo
     CDS             complement(5500..5688)
                     /locus_tag="IXF88_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5668..6240
                     /locus_tag="IXF88_001979"
     CDS             5668..6240
                     /locus_tag="IXF88_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1508701.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            6580..7434
                     /locus_tag="IXF88_001980"
     CDS             6580..7434
                     /locus_tag="IXF88_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1508702.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMIERI
                     IQLENEKE"
     gene            7533..7742
                     /locus_tag="IXF88_001981"
     CDS             7533..7742
                     /locus_tag="IXF88_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ1508703.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            7759..8031
                     /locus_tag="IXF88_001982"
     CDS             7759..8031
                     /locus_tag="IXF88_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1508704.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            8033..8896
                     /locus_tag="IXF88_001983"
     CDS             8033..8896
                     /locus_tag="IXF88_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAQ1508705.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(9159..9896)
                     /gene="erm(B)"
                     /locus_tag="IXF88_001984"
     CDS             complement(9159..9896)
                     /gene="erm(B)"
                     /locus_tag="IXF88_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1508706.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(10021..10104)
                     /locus_tag="IXF88_001985"
     CDS             complement(10021..10104)
                     /locus_tag="IXF88_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1508707.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         3581 a         1571 c         2117 g         2906 t
ORIGIN      
        1 ctacagtacc gataccaaca aggaaaggtg caggtcaacc ataagcgatt tatgggctac
       61 acgaaagatg aagatggaaa tttaactatt gtccccgaag aagctgagat tatcaaacgc
      121 atctaccgag aataccttga gggtcagagt ctagtgggca ttggtcgagc ccttgaaaag
      181 gatggtattt taacagcagc gggaaaacca agatggcgac cagaatcagt taagaagata
      241 cttcaaaacg aaaaatacat cggagatgcc cttctgcaaa agactgtcac agtagatttt
      301 ctgaccaaga aacgagtgaa aaatgaaggg catgttcccc aatattatgt tgaaaatagc
      361 catgaagcga ttattcctaa agagttattt ttgcaggcgc aagaagaaat tcatcggaga
      421 agcaatatct acacaggaga aggcaagaac aaaagaattt acagtagcaa gtacgcttta
      481 agtgccatca ccttctgtgg agattgtggc gatatttata gacgggtcta ttggaatatc
      541 cacggaagaa aagaatttgt ctggcgatgc gtgactagaa tcgagcaagg tcctgaagtt
      601 tgtaagaacc gaaccgtaaa agaagatgaa ctttatggtg cagtaatgac tgcgattaat
      661 aagctacttg caggtgggaa caatatgata aagacactgg aagagaatat tcatgctgtg
      721 attggcgaaa ccacagaata ccaaatttca gagattaaca actcactaga ggaaaaacaa
      781 aaagaactaa tcaagctggc gaacaagggt caagactatg accacttagc agatgagatt
      841 gatgagctga gagacaagcg acagatcctt ttagtagaag atgcctccct cagtggcgag
      901 aacgagcgaa tcaatgagct gattgaattt atccgcaaga acaaattccg caccttagaa
      961 tatgatgata agcttgtaag gaagataatc cagagcgtta cagtctatga aggccacttc
     1021 gtcatagcct tcaaatctgg catcgaaatg gaaatatgaa aaccagaagt aaatagccca
     1081 tgactctaca gtagagttgt gggttttctt ttgcttgctg attataataa tattttaata
     1141 actattgtgt ttatcaacca aatggtttat aatattctta gcgtaaactt ggaggtgctg
     1201 tatgactacg gcagaaatga ttaaagaact gtgtgagcaa atgaatataa gtgtttccga
     1261 acttgctaga cgtattggcc agactccaca gaatttcaat aaaaaattac aacgagaaac
     1321 agtaaccttg gatgagttga aggccattgc tgatgtgcta ggtgtcaagt ttgtgcaggc
     1381 atttatttta ccagatggag aagaaataaa aatatctaac gaataaagga ggatgtttat
     1441 atggtaggta atgttattca aatagtaaca gagaaattat catctttgcc ttttatagaa
     1501 ggcatcgtat tagggggctc acgtgcaaga ggcacccata cagaaaattc tgatatagat
     1561 atcggaatct attacaattc agactcattt gacctgacag cgattaatca aattgctaca
     1621 gagttggatg atgagaatag aaacaacctt gttgtacctc ccggagcgtg gggtgattgg
     1681 gttaatggcg gtggatggtt agttataaac gggtgtcatg ttgacttgat attacgtgat
     1741 ataaaacggg tggaacaaat aatcaaagat acggagcaag gaattgttac tgccaattat
     1801 cagaccgggc atccccatgg ttatataagt gctatgtatc gtggagaatt agcgattagt
     1861 aaaatactat atgctaagaa cgaaagctta tgcgaattaa aaaagcaggc agaaatttac
     1921 cctactgctc taaagaaaag cttgatgaac ttttttatat ttgaagctga attctcttta
     1981 atgtttgtaa aagcaaatgc gggggcagag gataaatatt atattgcagg ccatgttttt
     2041 cgtattattt catgcttaaa tcaagtacta tttgcatgta ataatgctta ttgcattaac
     2101 gaaaagaaag ctataaaact gcttgaaact tttgaatata aacctgaaaa atatgccgaa
     2161 agagttaatc atatttttga agtactcggt ctttctcttt ttgaatgcta cgacatgacc
     2221 gagaaacttt ataaagaagt gaatgaaatt gtatcggaga taaataactt tttaaacgag
     2281 gagagttcag atgaaagaaa acaaatatga tgataatata ttttttcaaa aatacagtca
     2341 aatgagtcgc tcgcagaaag gactggctgg tgcgggagaa tgggagactt tgaaaaagat
     2401 gctacctgat tttaagggta agcgtgtgct tgatttagga tgcggctatg gatggcactg
     2461 tatatatgcg atggaaaacg gtgcttcctc tgtagtaggt gttgatattt ctcataaaat
     2521 gctcgaagta gcaaaaggaa aaacccattt tccacagatt gaatatgaat gctgtgccat
     2581 agaagatgtg gatttcccag aggagagctt tgatgtaata ctaagttcgc ttgcgtttca
     2641 ttatgtagca gactatgaga atttaataaa aaagatatat aggatgctga aggctggtgg
     2701 caatttagtt tttacagttg aacatcctgt ttttactgct catggaacac aagactggta
     2761 ttataacgaa aaaggagaaa tactgcattt cccggtggac aattattatt atgagggcaa
     2821 acggacagct atgtttttgg aagaaaaggt tacaaaatat catagaacac tgaccacata
     2881 tctaaataca ctgctttcaa atagttttat aataaatcag attgtggagc cacagccgcc
     2941 agagaacatg atggatattc cgggggtggc ggatgaaatg cgacgcccaa tgatgctgat
     3001 tgtatcggca aaaaagaaga tgtaataata tagaaaaaat aaacgaggag tatgtaaatg
     3061 agatcagaaa aagaaatgat ggatttagta ctttctttag cagaacagga tgaacgtatt
     3121 cgaattgtga cccttgaggg gtcacgcgca aatattaata tacctaaaga tgaatttcag
     3181 gattatgata ttacatattt tgtaagtgat atagaaccgt ttatatctaa tgatgactgg
     3241 cttaatcaat ttgggaatat aataatgatg caaaagccgg aggatatgga attattccca
     3301 cctgaagaaa agggattttc ctatcttatg ctatttgatg attacaataa aattgatctt
     3361 accttattgc ccttggaaga gttagataat tacctaaagg gcgataaatt aataaaggtt
     3421 ctaattgata aagattgtag aattaaaagg gacatagttc cgactgatat agattatcat
     3481 gtaagaaagc caagcgcaag ggagtatgat gattgctgca atgaattttg gaatgtaaca
     3541 ccttatgtta ttaaaggatt gtgccgtaag gaaattttat ttgctattga tcattttaat
     3601 cagattgttc gccatgagct gctgagaatg atatcatgga aggtcggcat cgaaacaggc
     3661 tttaaattaa gtgtaggcaa gaactataag tttattgaaa ggtatatatc cgaggatttg
     3721 tgggagaaac ttttgtccac ctaccggatg gattcctatg aaaacatatg ggaagcatta
     3781 tttctatgcc atcaattgtt cagggcggta tccggtgagg tggcggaaag gcttcattat
     3841 gcctatccgg agtatgatag gaatataaca aaatatacca gggacatgta taaaaaatac
     3901 actggtaaaa ccggctgcct ggatagcaca tatgccgctg atatagaaga gaggcgggaa
     3961 cagtgattac agaaatgaaa gcagggcacc tgaaagatat cgataaaccc agcgaaccat
     4021 ttgaggtgat aggtaagatt ataccgaggt atgaaaacga gaattggacc tttacagaat
     4081 tgctctatga agcgccatat ttaaaaagct accaagacga agaggatgaa gaggatgagg
     4141 aggcagattg ccttgaatat attgacaata ctgataagat aatatatctt tactaccaag
     4201 acgataaatg cgtcggaaaa gttaaactgc gaaaaaattg gaaccggtac gcttatatag
     4261 aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat aggcagcgcg cttatcaata
     4321 tatctataga atgggcaaag cataaaaact tgcatggact aatgcttgaa acccaggaca
     4381 ataaccttat agcttgtaaa ttctatcata attgtggttt caaaatcggc tccgtcgata
     4441 ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt
     4501 tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta
     4561 tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc
     4621 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
     4681 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
     4741 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
     4801 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
     4861 gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
     4921 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
     4981 ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa
     5041 taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc
     5101 gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca
     5161 cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga
     5221 tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat
     5281 cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
     5341 gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga
     5401 tttagatgtc taaaaagctt taactacaag ctttttagac atctaatctt ttctgaagta
     5461 catccgcaac tgtccatact ctgatgtttt atatcttttc taaaagttcg ctagatagag
     5521 ttctatatta gagatgtaaa gagttttggt gaagaggttt gtgagtatgg cttttataac
     5581 cagggtgcaa tgagaagcac taacagcagc tagcttaaga acgcatagga tatacttttt
     5641 atggaagtca cagaggagga ataaaatttg aacaggatta acagagtaac ctctattctt
     5701 attcagctgc aatcaaaaaa aataattcct gccaaagaaa ttgcacagcg atttaatata
     5761 agcttaagga cagtttacag agatatccgg acacttgaag aagccggaat accaattgga
     5821 tctgaggccg gaaaaggata ttttcttgta gagggcttcc tacttccgcc ggtaatgttt
     5881 acagcggcgg aagtgggtgc attgattaca gcagggaaat ttttaaattg ccatggagat
     5941 gaatcattta taaaggattt tgattcagct atgtataaaa tcaaatctat tttgaagcat
     6001 ggtgaaaaaa actatgcaca ggagttggag aacagtatta acgtgtacag cacatctgga
     6061 cagaaaaata cattggcgga taacgttatt gcagcaattc agactgcaat ctgcaataaa
     6121 agggtaatat caattcaata tcctgcatca ggagggcaag aaccggaaag cagaatgata
     6181 gagcctatat cactggggtt ttatgaacag aactggtact taatcggttt tgcaggctaa
     6241 gaaatgaata taggaatttt cgtgtggatc ggattaaaag catttgcata gaagaagagc
     6301 tctgtcaaag ccatgatggc tctttggaac aaatattaaa acaaatgctt tcatataaaa
     6361 aactatatgc cctacaatgc cgttgtaaaa attggggaaa agggatatat tgattttata
     6421 tctttctcaa aataaatata aaagcccttt aaagagggct tttatatatt aatcacaaat
     6481 cacttatcac aaatcacaag tgattaatca caaatcactt gtgatttgtg attcttaatg
     6541 atacaatatt actatacaaa aaaagaatgg ggcgtagtta tggagaagga aaaactaaaa
     6601 atacttgaag aattaagacg tattttaaac aataaaaatg aagcaattat tatcttgaat
     6661 aattacttta aaggtggtgt tggaaagtcc aaattatcga ctatgtttgc ttacttgaca
     6721 gacaaattga atttaaaagt tttaatgatc gataaggact tacaggcaac attgacaaaa
     6781 gacttagcaa aaacttttga ggtagaattg ccacgtgtca atttttatga aggcttgaaa
     6841 aatggaaact tggcttcttc tattattcat ttgactgata atttagactt gatccctggc
     6901 acgtttgatt tgatgttact gccaaaatta actcgctcat ggacgtttga aaatgaaagt
     6961 agattgcttg ctactctttt agcaccttta aaaagtgact atgatcttat tattattgat
     7021 actgtaccaa cgccaagcgt ttatacaaat aatgcaatcg tggcgagtga ttacgttatg
     7081 atccctttac aagcagaaga agaaagtaca aacaacattc aaaactatat ttcctatttg
     7141 attgatttgc aagaacagtt taaccctgga ctagatatga ttggttttgt tccttattta
     7201 gttgatacgg acagcgcaac gataaaatca aacctggaag aactgtacaa agaacataaa
     7261 gaggataatt tggttttccg aaatattatc aagcgaagta ataaagtaag tacttggtct
     7321 aaaaatggca ttacagaaca caaaggctat gacaaaaaag ttttgtctat gtatgagaac
     7381 gtattttttg aaatgattga gcgaatcatt caattagaaa atgaaaaaga atagaatcac
     7441 aaatcacaag tgattaatca caaatcactt gtgatttgtg ataggtgatg ataaaataaa
     7501 tagtaagaag aaatagaaag aagtgagcga tcgtgggaaa tttaggcgca cagaaagcaa
     7561 aacgaaatga tacgccaatc agcgcaaaaa aagatataat gggggataag acggttcgtg
     7621 ttcgtgccga cttgcaccat atcataaaaa tcgaaacagc aaagaatggc ggaaacgtaa
     7681 aagaagttat ggatcaagcc ttagaagaat atatacggaa atatttacct gacaaacttt
     7741 aaaaggagtg aaaatgaaat ggcagttacg tatgaaaaaa catttgaaat tgaaatcatt
     7801 aatgagttat cggcaagtgt ttataatcgg gtattaaatt atgttttgaa tcatgaacta
     7861 gatactaaaa atactcgttt actagaagtg aatcttttaa atcaattaga agtggcacaa
     7921 gaagttgatt tatttcaaca accatttgaa gaattacaag ctattcatga gtattggcgg
     7981 tcaatgaatc aatattcaaa acaaattttg aataaagaga aagtggctta acatggcgaa
     8041 tattactgac ttcaccgaaa agcaatttga agatcgttta gaaaagaatg ttgaacgact
     8101 aactaaaaat agactagcgg ttgaatcgcc aaccgctttt ttacttggtg ggcaaccagg
     8161 gtcagggaaa accagtttgc gatcagcaat ttttgaagaa acacaaggga atgttattgt
     8221 cattgataat gataccttta aacaacagca cccaaatttt gacgaattag tgaaactcta
     8281 tgaaaaagac gtagtaaaac acgttacccc ttattcgaat cgcatgacag aagcacttat
     8341 tagccgttta agcgatcaag gctataattt agtgattgaa ggaacaggac gaacaacaga
     8401 cgttcccatt caaacagcca caatgcttca atctaaaggt tatgaaacaa aaatatacgt
     8461 catggcagta ccgaaaattg aatcatactt aggaacgatt gaacgatatg aaaccatgta
     8521 tgcagatgat ccaatgacag ctagggcaac accaaaacaa gcgcatgata ttgttgtcaa
     8581 aaacttaccg accaatttag aaacccttca taaaacgggc ttatttagcg atataaggct
     8641 ttacaataga gaaggagtga aactctattc aagcttagaa acgccttcca ttagtccgaa
     8701 agagacctta gaaagagaat taaatcgtaa agtatcaggg aaagaaattc aaccgacttt
     8761 ggagagaata gagcaaaaaa tggttcaaaa tcaacaccaa gaaacccctg aatttaaagc
     8821 aattcaacaa aaaatggaaa gcttgcagct acctacacca ccaataccca aaacacctaa
     8881 acttccaggt atttaaacct taaaaaagga aaagagtagt taccaaaaaa cggtaactac
     8941 tcttttttta taaaaacatt tcctcatgtt ggggtcccga gcgcctacga ggaatttgta
     9001 tcgataagaa atagatttaa aaatttcgct gtttttttct aatttcattt tgaggtgaat
     9061 taattggtag ttattagagg tgccctatac ctaataattt atctacattc cctttagtaa
     9121 cgtgtaactt tccaaattta caaaagcgac tcatagaatt atttcctccc gttaaataat
     9181 agataactat taaaaataga caatacttgc tcataagtaa cggtacttaa attgtttact
     9241 ttggcgtgtt tcattgcttg atgaaactga tttttagtaa acagttgacg atattctcga
     9301 ttgacccatt ttgaaacaaa gtacgtatat agcttccaat atttatctgg aacatctgtg
     9361 gtatggcggg taagttttat taagacactg tttacttttg gtttaggatg aaagcattcc
     9421 gctggcagct taagcaattg ctgaatcgag acttgagtgt gcaagagcaa ccctagtgtt
     9481 cggtgaatat ccaaggtacg cttgtagaat ccttcttcaa caatcagata gatgtcagac
     9541 gcatggcttt caaaaaccac ttttttaata atttgtgtgc ttaaatggta aggaatattc
     9601 ccaacaattt tatacctctg tttgttaggg aattgaaact gtagaatatc ttggtgaatt
     9661 aaagtgacac gagtgttcag ttttaatttt tctgacgata agttgaatag atgactgtct
     9721 aattcaatag acgttacctg tttacttatt ttagccagtt tcgtcgttaa atgcccttta
     9781 cctgttccaa tttcgtaaac ggtatcggtt tcttttaaat tcaattgttt tattatttgg
     9841 ttgagtactt tttcactcgt taaaaagttt tgagaatatt ttatattttt gttcatgtaa
     9901 tcactcctga agtgattaca tctataaata aatacagaag ttaaacgatt tgtttgtaat
     9961 tttagttatc tgtttaaaaa gtcataagat tagtcactgg taggaattaa tctaacgtat
    10021 ttatttatct gcgtaatcac tgtttttagt ctgtttcaaa acggtagatg ttttatctac
    10081 attacgcatt tggaatacca acatgacgaa tccctccttc ttaattacaa atttttagca
    10141 tctaatttaa cttcaattcc tattatacaa aattt
//
LOCUS       DACESX010000092        10115 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.092, whole genome shotgun sequence.
ACCESSION   DACESX010000092 DACESX010000000
VERSION     DACESX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.092"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..626)
                     /locus_tag="IXF88_001986"
     CDS             complement(<1..626)
                     /locus_tag="IXF88_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1508708.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="IXF88_001987"
     CDS             899..3370
                     /locus_tag="IXF88_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1508709.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="IXF88_001988"
     CDS             complement(3473..5209)
                     /locus_tag="IXF88_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1508710.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="IXF88_001989"
     CDS             complement(5206..5910)
                     /locus_tag="IXF88_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1508711.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="IXF88_001990"
     CDS             6041..6544
                     /locus_tag="IXF88_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1508712.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="IXF88_001991"
     CDS             6504..6842
                     /locus_tag="IXF88_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1508713.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="IXF88_001992"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="IXF88_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1508714.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="IXF88_001993"
     CDS             8393..8971
                     /locus_tag="IXF88_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1508715.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="IXF88_001994"
     CDS             8975..9496
                     /locus_tag="IXF88_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1508716.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10105)
                     /locus_tag="IXF88_001995"
     CDS             complement(9575..10105)
                     /locus_tag="IXF88_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1508717.1"
                     /translation="MGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLPFYNEDLET
                     PEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHSVWDHKPGL
                     VVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNIVEGTLSFF
                     QTILDAYLDFSNKLTE"
BASE COUNT         3257 a         1876 c         2101 g         2881 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatc agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaacgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atcca
//
LOCUS       DACESX010000093         9912 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.093, whole genome shotgun sequence.
ACCESSION   DACESX010000093 DACESX010000000
VERSION     DACESX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.093"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            201..680
                     /locus_tag="IXF88_001996"
     CDS             201..680
                     /locus_tag="IXF88_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508718.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="IXF88_001997"
     CDS             713..2941
                     /locus_tag="IXF88_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1508719.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="IXF88_001998"
     CDS             3070..4713
                     /locus_tag="IXF88_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1508720.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="IXF88_001999"
     CDS             complement(4786..5331)
                     /locus_tag="IXF88_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1508721.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="IXF88_002000"
     CDS             complement(5354..5839)
                     /locus_tag="IXF88_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1508722.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="IXF88_002001"
     CDS             complement(5829..6641)
                     /locus_tag="IXF88_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1508723.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6780..8312)
                     /locus_tag="IXF88_002002"
     CDS             complement(6780..8312)
                     /locus_tag="IXF88_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1508724.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8421..9452)
                     /locus_tag="IXF88_002003"
     CDS             complement(8421..9452)
                     /locus_tag="IXF88_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1508725.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3297 a         1836 c         1905 g         2874 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc attgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
     6721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
     6781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
     6841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
     6901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
     6961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
     7021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
     7081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
     7141 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
     7201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
     7261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
     7321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
     7381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
     7441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
     7501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
     7561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
     7621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
     7681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
     7741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
     7801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
     7861 gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc atttgaaatt
     7921 tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc taaacgatcg
     7981 ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg cactgcacca
     8041 aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt ataaataata
     8101 actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt caacacactt
     8161 tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga aagtggctca
     8221 tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag
     8281 ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt tgttaagtaa
     8341 tatacaaatt caagataatc gtaacataat cctttatata ataacatagt ttattgtgaa
     8401 aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc taattcgtga
     8461 taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga gaggtatcga
     8521 ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc ataataacga
     8581 tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt gctcaaaatt
     8641 tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac aaaagaattt
     8701 tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat cgcttctttt
     8761 ggtattggta ctttattcag tctgttttct tttctattca gattaagaat ctgcttggtc
     8821 aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc gcaatacaga
     8881 agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct agcttctctg
     8941 cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa aagttctgca
     9001 tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa aagagaaatc
     9061 tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc
     9121 ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg gttcgctgta
     9181 gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata atcttgtttt
     9241 tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct caaataatca
     9301 gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag ggaagaacga
     9361 ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg ctttaacttt
     9421 tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata gtgttcattt
     9481 tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat cttaggaagc
     9541 aaatttttaa ctaagtacta gctaaaagat atgaagtaag aagatacatt tttagttatt
     9601 ttattagtgc agtcattctt ttgatctttt aattgattta tcctcttttt agctatgtgg
     9661 ttacttcatg agcaatgaaa tggatttggt tcgttctaat ggcaggaggc tctttctttt
     9721 atttccgaaa aattagaaaa cattttttca gaatcgattg gctctccatt tattcaataa
     9781 tggtttgtct aacgatattg ttgggataat tcttaaagaa gtattagaaa tagttggtac
     9841 ttcattactt tacatgaaga tttattctat ggtaggagca attatattga gaattaatcc
     9901 ttttttatat aa
//
LOCUS       DACESX010000094         9720 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.094, whole genome shotgun sequence.
ACCESSION   DACESX010000094 DACESX010000000
VERSION     DACESX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.094"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            280..1254
                     /locus_tag="IXF88_002004"
     CDS             280..1254
                     /locus_tag="IXF88_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1508726.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            1276..1968
                     /locus_tag="IXF88_002005"
     CDS             1276..1968
                     /locus_tag="IXF88_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1508727.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            1934..3442
                     /locus_tag="IXF88_002006"
     CDS             1934..3442
                     /locus_tag="IXF88_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1508728.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            3516..4619
                     /locus_tag="IXF88_002007"
     CDS             3516..4619
                     /locus_tag="IXF88_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1508729.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            4622..5959
                     /locus_tag="IXF88_002008"
     CDS             4622..5959
                     /locus_tag="IXF88_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1508730.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            5946..6824
                     /locus_tag="IXF88_002009"
     CDS             5946..6824
                     /locus_tag="IXF88_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1508731.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            6821..7744
                     /locus_tag="IXF88_002010"
     CDS             6821..7744
                     /locus_tag="IXF88_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1508732.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            7741..8151
                     /locus_tag="IXF88_002011"
     CDS             7741..8151
                     /locus_tag="IXF88_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1508733.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            8155..>9720
                     /locus_tag="IXF88_002012"
     CDS             8155..>9720
                     /locus_tag="IXF88_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1508734.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT         3160 a         1602 c         2120 g         2838 t
ORIGIN      
        1 aataaattaa atgtttctgt ttctatatgt tcaatttatc atcggtgaaa gattacgtat
       61 ataatcattt cctatgctaa gtgtcagata gaaaaaaata gaaggtttgc tatgcgtagt
      121 ttatttgtga aaaaatacac tttatcgttt tgtatagcac aaaaatcatt gctatgtaag
      181 cgtttttact tacggtaaaa atgattatat acgtaattca gatagttatc tatcatgaag
      241 tcataagcag caaacattca caaaactaag gaggggctta tggaaagtgt aaagaaacaa
      301 gaaccatttc aactgacgct atcgaaaaaa gaagaaaagc tcagaagacg aaaaaaaata
      361 attgcaggaa tcaaaacgaa taaattttta tatgtgatga ttctgccggg agtcctttat
      421 ttcttgattt ttaggtatct tcctatgggg ggactcgtta ttgcttttca agattatcaa
      481 ccatttttag gaataatggg aagtccttgg gtcggtttca aacatttcat tcgtttgttt
      541 acagaaccga cattctttac attattaaga aatacgttga tcttgttcgg attaaatatc
      601 ttcgtgtttt ttccgatgcc aatcattttg tctttactat taaatgaagt aacgaacaaa
      661 cgattgaaaa acgggattca aacgatcatt tacatccctc acttcatgtc ttgggtaatc
      721 attgtttcga ttacttatgt atttttgaat gttgacggag gggtattgaa tgaattcctt
      781 gcaacaatgg gatttgaaaa aatcagtttt ctaacatccc ctgaatggac acgcacagta
      841 tatatcggac aagtgatctg gaaagagttg ggctggtcta cgatcatcta cctttcagcg
      901 ataactgcag tcgatacaca gttgtatgaa gctgcagaaa tggatggagc aggacgctta
      961 cgcaaaacat ggcatgttac attgccggca attcgtccag taatcattac cttgctgatt
     1021 ctaaaaatcg gtcagacatt agacttaggg ttcgagcata tgtacttgtt attgaattca
     1081 ttgaatcgaa gcgttgcaga aattttcgac acctatattt atacagctgg attgaagaat
     1141 ggacagttaa gtttcagtac agcaatggga ttgtttaaag gggtcgtagg attgtttatg
     1201 gtaattttag ctaataagat tgccaaaaag ctaggagaag acggcgttta ctaagataga
     1261 aagagaggga aagatatgct gcagtcatta gataaaataa acggaaggct aaatcgtatt
     1321 ttacagttgg tttatttgaa ttttctgtgg ggcggtttag tcatcgcagg tttgggagtt
     1381 tttactttcg gtcctgcatc gtatgcgatg gttgcagtga ttagaaaatg gctgcgctca
     1441 aaagaaacgt ttcctttgac gaaaacgtac ttcacatatt ttaaagaaaa ctatcgtgaa
     1501 accttgctga tgagctggat atatggcgcc atcgggattg ttttacttgt ggatctttct
     1561 ttcatacaca attggtatct taggatcgct ttgtatattg ctgcgttcat atatgtgctc
     1621 tcagcactct atattttccc aatcatggct cattatgatt ggaaaggata ttttttcaaa
     1681 atgaaaatgg catttttatt cggattctca catcttcatt actcattagt gttgatatta
     1741 agtatggtag ggatttatct gatgatcatc caaacaatgc caggtgtttt aacctttatc
     1801 ggtggcagtc tcttcttttc cgcagtcacg tggacagcaa attctatatt ttgccatttg
     1861 gaatggaaga actcatatac agaaaaaaca gaagaaaaca aaaatattat taaaacagtg
     1921 aaggagaaaa tttatgaaaa aagcaacgct cgtaaaatta ggtcttagtt ttgcaggaat
     1981 tctagcactt gccgcatgcg gcagcagttc aagtaacagt tcttcagaga cagaagatgg
     2041 aaaagctaaa atcacttggt taaatatttt gcatcatgct tcaccgccta cagacacgat
     2101 tgtgaacttg atcgaagaaa aaaccgatac ggaactgaca ttcaactgga ttcctgatgc
     2161 ttcaaaagaa gaaagattaa caactgcatt ggcttctgat gaactaggag atatcgttac
     2221 acttcaaatt gcgatgttga aaaactcttc tgtccgcaat gcgttaaaat caggcgtatt
     2281 ctgggatgta tcagaatatt taaaagacta taaaaattta agtcaaattt ctgaagatag
     2341 aatcgaagct gcgagtatcg acggtgcctt gtacggcgta ccgatacaaa aagactacgc
     2401 tcgtgctggc ttagtcattc gaaaagactg gttggataat ctaggactag atgttccaac
     2461 taccatggat gagctttatg aagtagctaa acaatttaca gaaaacgatc ctgatggaaa
     2521 tggtgtggac gacacagttg gttttggcga tcgtgcgcct agtgatattc gttataccag
     2581 ctttaaactt ttcacttctt attttggagc accaaatggc tgggctgtag acgataatgg
     2641 caaattcact ccagaatttg atacagaaga gtatatgaaa gctttgaatt tttcaagaga
     2701 tctatttgag aatggctatt tggcacaaga ctttgcagtt acccaaaaga ctgatcagca
     2761 agaacaattt gcacaaggaa gaactggaat ctacactgga atggtggata tcaagaatct
     2821 gaagacgatg ggtcaaggta ttcaagatga tatggaatta gtgcctgtaa acaagatctc
     2881 agatggtgaa ggcggcggtt atcatgtttg gtcagaagga aatggtattg gcggcttgct
     2941 agcatttccc aaatctcaag taaaagacga agcacaattg aagaaattac ttcaatttgt
     3001 tgatgatctt ttagaagaag atgtgtataa attgatgact ggcggtatcg aaggaacgca
     3061 ttatgaattg aatgatgatg gttctatcga atttatcgat atggatcttt ggcaacaaga
     3121 ggtgcaaccg ttatcaagct ctcgtcctag tgaagtgacg ttctctttca aagatacaga
     3181 ccctgaaaaa gaattctcta atcaattgat caaagaaaat gaagaatttg ctgtaatgga
     3241 tccttctgtg ccattggatt caccgacgaa caatgaagta ggatcagaaa ttgaaaaaat
     3301 cgtcattgat gctacggtgc aatacatcat gggacagctt gacgaagatg gattcaaaaa
     3361 agctgtagaa gactggaaag cacaaggagg cgatcgaatc acacaagagt acgaggaagc
     3421 atacaaagca gcggcacagt aatatagacg attgggacaa atacttcttg gtatttgtcc
     3481 caaatcctat tttaattttt aaaggagagc aaataatgga aaactggtca aagaaattag
     3541 caaaaagtat catggaaaga acacctcgct tatatgaaga aaagtggtat aaaggaaaat
     3601 ggtcttatga ctatggcgtc gtacttaaag ggttccaatt actttgggaa cgaacccaag
     3661 aaaaaattta ttttgatttc ataaaagaca atatcgacta tttcgtgcaa gaagatggaa
     3721 cgatccgtgg ctacagcgtt gaagaataca atatcgacca tgtaaataca ggaaagcttt
     3781 tctttttatt gtacaaagaa acaggcgaag aaaaatataa aaaagcagca gaactccttt
     3841 cccagcagtt ggcaaaccat ccacgcacat ccgaaggagc tttttggcat aaagaaattt
     3901 atccttatca aatttggttg gatggcttat atatgggttc tcctttttat gctgaattta
     3961 tccagaattt tagtgaagaa aaagattacc aagatgtttt gagacagttt gaaatcagtt
     4021 ataaacatct attcgatcca aaaacaaatt tgctgattca tgcatgggat gaaaagaagg
     4081 tccaaccatg ggctgatcca aaaacaggtc tatcccatca tttttggagc cgatcgattg
     4141 gctggtattt gatggctgca gcagatacct atgaattact tcatgaagaa acagattgtt
     4201 ctttgctgaa agaaattcta gaaaagacac tggctgcatt acttccatat caagaagaaa
     4261 acagcgggac atggtatcaa gtaacaacag aaacaaaacg gaaaggaaat tatctggaag
     4321 cttccggcag tagtatgttt gtttacgcaa tggctaaagg aatacgtttg ggactgctga
     4381 acaaagaaga atggcttcca caagtgaaga aatcacatca aggattactt gatgaatttg
     4441 ttctggaaac aaaagaaggc tggctgaact tgaataaaaa ctgtcaagta gcagggttag
     4501 gcggagcaga tcagcgagat ggttcatatg cttactacat cagtgaacca attatttgta
     4561 atgatcaaaa aggcgttggt gcattcttgc aagcattgat tgaagtggag gcattataaa
     4621 tatgtatgat attttgacat ttggcgcatc aattgatgaa ttgaatactg aagcaataca
     4681 acaagccatt gatgctgcag caagtgacgg aggcgggacc gtagttgttc cagcaggtga
     4741 atttctaaca ggagctttat ttttaaaaag caatgtcgaa cttcatttaa gcgcaggagc
     4801 ggttctgaaa ttctctgacg atccaaaaga ttatcctgtt gttcattcac gttgggaagg
     4861 agtccatcga aaagtttatg cttcctgtat ctatgctcaa aatgtagaga acatctctgt
     4921 cactggtttc ggtacattag atggaaatgg aaaaaaatgg tggcatactt ttagaaatga
     4981 accagataat ctcgcttatc ctcgtcctaa attgatgagt ttccataact gtcatcgaat
     5041 tactgtgaaa gatattaaat tgatccagtc accaagttgg acgatcaacc cgattctttg
     5101 cagtaatgca acgtttgata acttgacgat cttgaaccca gcggattcac caaatacaga
     5161 cggaattgat ccagaatcat gcaaaaatgt gcgtattagt aattgccata ttgacgtggg
     5221 agatgactgt attgcaatca aggcagggac tgaagatacg tacgaaagga tcgcttgcga
     5281 aaatatcacg atcacaaatt gcacgatggt ccatggacat ggcggtgtcg tattaggaag
     5341 tgaaatgagt ggtagtattc gtaatattac catttcaaat tgtatcttcc aagaaactga
     5401 tcgaggaata cgattgaaat ctagacgagg tcgaggcggg atcgttgaag atattcgagt
     5461 atccaatata gtcatggata atgtgatgtg tccatttata ctgaatcttt attatttctg
     5521 tggtccgcga gggaaagaac cgtatgtatg ggaaaaaaag gcttatccga tagatgaacg
     5581 gacaccagca ttcagaagga tccatttttc gaatatcacg gcaaggaatg tccatgcttc
     5641 tgcaggtttt atttatggat tagcggaaca attcatacaa gaaatcacat ttgacaatat
     5701 cgatgtctcg atggcccaag atgcagttcc cggtgttcct gctatgatga caggaataga
     5761 ggaaatgagc agacaaggat tttatattgg ctttgcgggt aaggtttcct ttaaccagat
     5821 gtcgattgaa aatcacgagg gaccagcatt tctattagaa aatactaaag aaatcagtat
     5881 tgagaaattc caatcaaatc aatcagaaaa tatggatgaa ctggttgttc gtaaaaactt
     5941 tgcttatgca gttgaatgat tttttaggtg ctccatctag cagacatacc cctagtggaa
     6001 aacgattata ccgagaaaaa caaaacggtt ttatgcttga atactatcaa ttgtacttaa
     6061 atgaattgga aacagtacca gccctctatg cttatccaga aaaagaagga ccgtttccgc
     6121 cggtccttta cctgcattct cacggcggag atttttcagt cgggaaaagt gaattgatcc
     6181 atggtgctcc ttacttagct tctccatctt ttgcagaagt actgacagga atgggatatg
     6241 ctgtttggtc gattgatgct tggggatttg aagaacgcgg aggtatttct gaaagcgagt
     6301 tgttcaaaca atttttatta acaggaaaaa cgttatgggg aatgcgtatt tatgatgtga
     6361 tcagcttgat cgattatttg gagacaaggg aagacacaga tactcaacgg ctcgcaacga
     6421 ttggtctttc tatgggaggc ttattaagct ggtgggtgac agcattggat tctcgtgtca
     6481 aagtttgtat tgatctagcg gcacaggtgg acatcgaaat acttttgcta catcgccgcc
     6541 tagatcatca tgggttttat tactatgtgc caaatctatt gacagcttat acaacgttag
     6601 caatccaaga aaaaatcgct ccgcgccctc gtcttagctt agttggaaaa aacgatacga
     6661 tgtgcttgga cgaaggcgta ctgaagctga gaaaagggct ggacaaaaag tatcaatcaa
     6721 tgggtagtcc agaaaacttt tcttcttttt ctttaacagg cggtcatatg gaaacacaag
     6781 aaatgagaaa tatttggaaa caattcataa gggagcattt atgaggatga aagacaaact
     6841 gataattgga aagaatgcta tagatacccc gacggatttt gaaactatcc aagaaggaat
     6901 cgattatttg gcaggcctgc cggatagcca tccaaaggtc ttgatagtaa aagaaggaag
     6961 atatgatgaa tatatagaaa gcagattatc caattttaaa cttgtgggag caggaacagt
     7021 agttatctcc ggtaatcgtt ttgcaaaaca ggagtatctt gacggtagcc agttagggac
     7081 tttccgaagt gctacttttt tcttagaagg atcgaatgtc gtattagaaa atcttcacat
     7141 cgaaaataca gcaggtccag gagaaaaggt aggacaggct gttgctttat tcaattcagg
     7201 agatcgcgta tctttaaaaa actgtcgact atctggttat caggatacct tatgcacggg
     7261 tccgttgcct gatttgcaaa aagatggtac tcctttttgt acaccggctt ctgccaatcc
     7321 tgagtattgt cgtcagacct atgaatattg ctggattgaa ggaacggtgg atttcatatt
     7381 tggtggggca gatgccgttt ttcatcagtg tcatatccat agcttagcca gtaaaaatcc
     7441 tggatacatc acagcagcat ctacgccgaa aaatcaaaaa acagggtatc gttttgaaag
     7501 ctgtaccttg actgcagaac ctgatactag aaacgtctat ttaggaagac cgtggcgtcc
     7561 gtatgcaaat gtcttatttg aagcgtgtaa gataggtggg catgttcacc caactggttg
     7621 ggataattgg gatgacaaga aaaatgaaca gactgtcaaa ttctctgaac gtaataacaa
     7681 ttatgaaaca tcgatcaaaa gacctgaatg gatacattta gaaaagcagg gggttcagaa
     7741 atgaaaagaa aatgggaact attgctagga atggtcggtg gcagtctatc tttgattttc
     7801 tttggcgggt tagcagttac tttaagtaat atgtctgcgt cagagtttaa aaaaagttat
     7861 cagtcgcttg ctgtagatca ttcaactttg tctttagaaa atacatttgg attattacag
     7921 gacatgactg gtctgtttgc cgttgtgttg tttatttcac ttgctttttt agcagtagca
     7981 ttgtttttga ctgctaaagg aaaatactta acaactgcca ctggtttgta ttttataaca
     8041 ggattcatat tgttgattgg cactcagttt attgcgtttc cttttgcatt tttttatttc
     8101 gcagctgggg cattcagttt atatcgagta agaatgagaa aaggagcctg aatcatgcag
     8161 actatcaaag tgaaaaatcc tattttaaga ggattcaatc cagatcctaa tatattaaga
     8221 gtgaaagagc ggtattacat tattgtctcc tcttttgaat ggcttccagg aatccgagta
     8281 tatgagtcga aaaatttagt caactggtca catacaacgg atattttgac cactcaagta
     8341 tctttgcaag gaaatcctac aaatggaagt atatgggcgc cacagatcag ttatgctgat
     8401 ggattgtttt atcttgttta cacagatgtc aaaagtgcag gccgtccgtt taaagatgtc
     8461 cacaattatt tgatcactgc atctgagatc acaggtcctt ggtccgaacc aatttattta
     8521 aatagtaacg gctttgatcc ttctttgttc catgatcttg aaacgggaaa aaaatggctt
     8581 gtcaatgaac tatgggatta tcgtctggac acaccgaata aatccagcgg tatcgtatta
     8641 caagaatatg atgcagagaa gaagaagctt gatggtccca tctataaaat atttgcaggt
     8701 acagaattag cgaaaacgga ggcaccacat ttgtattttc gtaacgggta ttattatttg
     8761 atgacggcag aaggcggtac aggaagagga cattcagtta cagtttgccg agcaaagaat
     8821 attactggtc cttacgagct tgatcctggt tttcctatgc tgacagctag tgacaaacct
     8881 gcttctacat tgcaatgtac aggacatggg agcttagtac aaaccccgtc aggaagatgg
     8941 tacatgacat atctttgtac cagaccatta aacggtgcgg ctattttggg aagagaaacc
     9001 gctattcaag aagtgtattg gacgtccgac gattggttaa gacttaaaga tagccagaca
     9061 actccgatgg aagaaataat gattgagaca aatgaagagg tacaacaaga aaaagatcat
     9121 cagtttatgg atacatttga aggagagcta aagaagaact ggaatgccag acgcattttg
     9181 ccaaatgctg attggtgtga tacaaaatgt agaccaggtt ttttacgtct gatttccggt
     9241 gaatctcttc aatctacctt cgaacatcat ttgttagcta ttcggcaaac ggattttact
     9301 tttgatgcgg agacagcatt tgaatacatg cctaataatt ttaatcagat ggctggactg
     9361 gtactgtact taaatgaatc caattatatt tattgttatg ttacttggaa tgaggacaag
     9421 ggaaaatgcc tacgtatgat agaatcagaa aacggagtag cacaaataga agattggacg
     9481 attccactca cagaaaagaa gacttttttg aaagtcactg taaagaaaga gacagcacag
     9541 tttcatttga aagacgtatc aactgaagaa tggaagaaag taggtccgca aaaaaacatg
     9601 tgtatactct caggagggtt tactggaaat tttatcggga tagcagtgca tgatctaaat
     9661 aaaaaaagag gaagttatgc tgattttgag tttttcaagt atgatggaaa agatcatctg
//
LOCUS       DACESX010000095         9696 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.095, whole genome shotgun sequence.
ACCESSION   DACESX010000095 DACESX010000000
VERSION     DACESX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.095"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            21..644
                     /locus_tag="IXF88_002013"
     CDS             21..644
                     /locus_tag="IXF88_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508735.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1094..2143
                     /locus_tag="IXF88_002014"
     CDS             1094..2143
                     /locus_tag="IXF88_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1508736.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2140..3021
                     /locus_tag="IXF88_002015"
     CDS             2140..3021
                     /locus_tag="IXF88_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508737.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3024..3794
                     /locus_tag="IXF88_002016"
     CDS             3024..3794
                     /locus_tag="IXF88_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1508738.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4073..4927
                     /gene="aroE"
                     /locus_tag="IXF88_002017"
     CDS             4073..4927
                     /gene="aroE"
                     /locus_tag="IXF88_002017"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1508739.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5105..6268
                     /locus_tag="IXF88_002018"
     CDS             5105..6268
                     /locus_tag="IXF88_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1508740.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6385..6795
                     /locus_tag="IXF88_002019"
     CDS             6385..6795
                     /locus_tag="IXF88_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1508741.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6969..9197
                     /gene="topB"
                     /locus_tag="IXF88_002020"
     CDS             6969..9197
                     /gene="topB"
                     /locus_tag="IXF88_002020"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1508742.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9335..9652
                     /locus_tag="IXF88_002021"
     CDS             9335..9652
                     /locus_tag="IXF88_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508743.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
BASE COUNT         3362 a         1646 c         2085 g         2603 t
ORIGIN      
        1 gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt agttaaatcg agagtacaag
       61 aaatgaatga acatttctca gtgtcattcg cagaaaatcc aagtaagtat tttcgacgtc
      121 ttgtttttct gctcatctta tcattgctgt taacagttgc aatgttttta ttgccggaaa
      181 aaatccgaga agagcaagta aatagtctcg tttattcgtc cataccaacc agcaaattga
      241 aaccttttga ttacagcaaa gctgatcaga aaatcaaaca aagccgggcg atatcggtga
      301 tgttcagcaa accgaacggg aaaacctatc aggacatgct tgatgttttt aatgatcctg
      361 agcttatgga agaattgaat cgaccgatct tttattatcc gatcgtttat gatgtacatg
      421 aactggaaca aaaatataac atacgaacgg atgaagtgac atttattttc tttgatggag
      481 gaaaagaagc aaatcggata actgcgggta agggaggaat aactgatttt gataaggagt
      541 tgatccctga actgaatcga cttccgttag ccaatatcaa acaattagaa gaagaattgg
      601 atagaacggg agtaacttcc caaaattccg agattgctaa ttgagacatt ggcgcgatga
      661 aacggtgggt ctatatagcg ctatttttaa ttatcagaac aagtgatgtt tgatcttcaa
      721 aataacaaat aaaaaattaa aaaatatagt tgacgaaatt caaaaattcg attagactta
      781 aatcaattct taaggaaagg gtgacctgta atgaacttat caaaacgtat ttcagcagaa
      841 aacaaaaaaa attggcgcag ctggcgtaga taagttgtat taatggtgag actttcccat
      901 agatacaact ttttagagtg catttctaaa gcttgtatct atgctagaca tgaacattga
      961 ctgccgcata gattagaatc tatgcggttt tttgtgtaaa gacaatgacc gctttggcca
     1021 ttgtcttttt ttgtagactg gatacttgcc tgattataat aaataaaaag attgactttg
     1081 caggaggaaa aaaatgaaaa acaaacgatt aataacagtt gtagtaatta ttttagttta
     1141 tttagcaggt gcatttattt atggaaaagc ctcaggcgaa aaagaaagta cttcgacgaa
     1201 ccagacaaaa aaaacaacag ttgttggggt attgcaatac gtgagccatc cggcattaga
     1261 tgagatttat cgaggaatca aagatggtct gaaagaagaa ggattaaaag aaggagaaaa
     1321 tcttgagatc aaatttcaaa atggacaagc cgatcagagc aagctagcca caatgagtca
     1381 acagctagta caatctgatc cagatgcctt gattggaatc gcaacacctg ccgctcaatc
     1441 attagcaaac acgacttcat cgatcccagt tgtgttaggt gctgtaacgg atcctgtggg
     1501 ggctggatta gtcgcttctt tagagaaacc tggtggtaat gtaacaggtg tttctgatca
     1561 gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg ttgccaaatg caaaaaaagt
     1621 cggaatcttg tatgcttctt cagaggataa ttcaaaatac caagtagcac aagcagaaga
     1681 agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg gtgccttcca ctaatgagat
     1741 tgcacaaacg gttcaagtga tgagccgaca agtcgatttt atctatgttc cattagataa
     1801 taccattgcc aatgcgatgc aaacagtggt aaaagaagca aacaaagcaa atatccccgt
     1861 cattccatct gtggatacaa tggtggaaca aggtggacta gcaacaatcg ggatcaatca
     1921 atatcaatta gggcttcaaa gcggtaagat ggcggcagaa cttgcgagtg gaaaagaaaa
     1981 accggaaacg acgcctgttt atacatttga tcaaggggat acagtgatca atcaatctca
     2041 agctgatcgc ttaggaatca ttgttcccca atcgatgaag gaaaaagcca aaatcattac
     2101 agatgactcg cagcaagaaa catctaagga ggatgacaaa tgatcgtatc agctatttca
     2161 caaggcatgc tttgggcaat attaggatta gggattttca tgacttatcg gatcttagac
     2221 tttcctgata tgacgacaga agggtccttc ccattaggtg ctgctgtctg tgtgacagct
     2281 attactcatg gggtagcgcc gattgtagct acattattag gcgtaggtgc tggcatgata
     2341 gcaggactcg ttacggggtt actttataca aaaggaaaga taccggttat tttagcaggg
     2401 attttagtga tgtcaggatt gaactcagtt attttgtttg tgatgcgaac accaaatctt
     2461 tctttattga atcatccgat tctacaagac agcttccatc gctttgggtt acctgattac
     2521 tttgacacag tccttcttgg cttcatctct ttgggcatct tgattgctgt actgctattc
     2581 ttcttcaata cagatttggg tcaaggctat attgcgacag gagacaacga aacgatggct
     2641 cggtcgttag ggataaaaac cgatcgtatg aaaattttag gtttaacttt gtcgaacggt
     2701 gtcattgcat tatccggtgc gctgattgcc cagaacgacg gatatgcaga tgtaaataaa
     2761 gggattggcg taatcgtcat cggtcttgct tccatcatca tcggtgaagt gatcttccaa
     2821 gaattgacgc tagcagaaag attgattgct attgttgtcg gcagtatcat ttatcagcta
     2881 ttgattttag tagtaatcaa actagggttt gacacgactt atctgaagtt gttctccgct
     2941 atcatcttag ctatttgtct gatgatcccg caattgaaga aagcactgaa cttaaaaacg
     3001 ggttttgaga aggaggcata gtaatgactg atatattgac aatcaaaaat ggcagaaaag
     3061 tcgtaaacaa tggaatgaat gaagagaaag tactattgga tgaaattaac cttacaatca
     3121 gagaagggga cttcatcact gtccttggcg ggaatggtgc tggaaaaagc acactgttca
     3181 atacgatcgc tggtacgctt caactcagtc aaggatcgat ctcttttaaa gatcagctga
     3241 tcacaaaaga ttcagaagaa aaaagagcac gattcctttc tcgagttttc caagacccca
     3301 agatgggaac ggctcctcgg atgacagtag cagaaaattt gctacttgca caaaaaagag
     3361 gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca aaaagaagat tttttcagac
     3421 tatgtcaaga agtcggtaat ggcctagagc aacacttgga tactccagcg ggtgaattat
     3481 ctggtggtca acgccaagca ttaagcttgc tgatggcgac gatcacagct cctgagttac
     3541 tattattaga tgaacatact gcagctttag atcctaaaac agctaaagca ttaatgcgat
     3601 taacaaatca gcggatcaat gaacaaaaat tgacttgtct catgatcacg catcgaatgg
     3661 aagacgcctt gaaatacgga aatcgtctga tcgtactcca aaaagggaaa atcgttcaag
     3721 atatttctga aaaagaaaag caacatttga caatgggcga ccttttgcag ttctttgaag
     3781 aagtagtgga ttgatttcga cagaaaaatt cctttcagta gaaggacttg aaaactcatt
     3841 gacatcttcc tttgattcat cctatactta aaacaataaa caattaaaca tatttgggtg
     3901 ttttgtagga tcacgtagtc aatgatttta acaattaaat agaatattct ttgctctgac
     3961 aagtaaacga tgagaaaaga atttgctact ttgccaccta gacgtttcgt actttttcgt
     4021 atcaaacgct aagtggcttt ttttattgaa gtaagaacaa aggaggaata agatgatttc
     4081 tggacaaacc aaattagctg ggctctttgc tgatccggct tctcacagtt tatccccatt
     4141 gatgcacaat actgcttttg aagcaaacaa catcgatgcg gtgtatttag ctttccaagt
     4201 aaatccaatg aatttgaagc aagcagtcga aagtattcga acttttgata tgttaggtgt
     4261 aaatctttcg atgccgaata aaacagcggt tattccttat ctggatgagg taagccagga
     4321 agcgcaattg atcggagcag tgaatacgat cgtgcacgaa aagggtcgtt tgattggcta
     4381 taataccgat ggtgcgggct tcatgcaatc cgtgcaagaa gctgggattt cgatcaaaaa
     4441 gaaaaaaatg actctactgg gagctggagg tgcagcaaag gcagtcactg ttcaagctgc
     4501 gttagacggc gctgaggaaa tcgtagttta taaacgaaac aaccaaacat tcgctcaagt
     4561 aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt aagataaaag ttcgtgactt
     4621 tgccgatacg catcaattaa gaaaagactt gaaagaaagc tgtcttctag tcaatgcaac
     4681 agatatcggg atgggaaaga aaatcaatca gtcaccgctc ttagatataa gcatgatgca
     4741 tgagaatcta gcggtttttg atttgattta tgcacctaga gaaacacgtt tattaaaaga
     4801 agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc atgttgatct accaaggagc
     4861 aatcgcgttt gaattatgga caaaggaaaa gatgccgatc catgtgattg aaaagctgtt
     4921 tgattaaaaa aggataatag ataaagatct tttattctcg cataaacggt ctcaagaaaa
     4981 gtaatccttt atgtatcgaa gcatttattt cgttattttt tattaaatta tcaaaaaatg
     5041 ataggctttt gtcgattggt tatcgtatcc tttatttatc agtaagaaac gaggaggaac
     5101 cacaatgagt cggatggatc gctataagga catccataag aaggcgaaac ctctgaagcg
     5161 ggatacgggg agtatttttg ctagaagaga acgaaaacga acgcgtatgg aaaatgagga
     5221 agatgctacc cgtatttatg aaagagagca gcattcattt gaacatttga accaatacga
     5281 gcaacagata tacagagatg caccaagagc agaagaacaa caaaaaaata aaaaattttt
     5341 caatcggcaa aagaagaaca aaccgttaaa acctcgaaga aagcgatcgt ggaaaaaaat
     5401 cattctatcg attttgcttg ttttgatcct ttattcaggt atctcatttt ttattggtaa
     5461 atatgtagct gaacatgaca caagcttaga ggcagcgact tcagaaacct ttcatgggca
     5521 aaaagcgacg aatggtgcgc ataatatttt gatccttgga agtgatacac gcggagaaga
     5581 tgccgggcga gccgatacga tcatggtctt acagttggat ggtccttccc ataagccgaa
     5641 actgatttct tttatgcgtg acagcttcgt tacgatccca ggggtaggac aaaataagat
     5701 caattcagct tatgcatacg gaggagccga tctagtgcgt caaactttag tagaaaattt
     5761 tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa tcgtttgaaa aagtgatcga
     5821 tgccttattc atgaacggag tgaagatcga tgcagaaaaa gatctgaatc tggatggtgt
     5881 agatattaaa aaaggggtcc agaagatgga tggacatgtt cttttgcagt acgcacgttt
     5941 ccgaatggat gaacaaggag actttggccg tgtcagacgc caacagcaag taatgaacgc
     6001 tattttcagc caattgaaga atccattgaa tctgatccgt gcaccttatg cagctggtaa
     6061 agctttagga tatacatcca cagatgtcac tgcttggttc ttgacgaaaa acctcttttc
     6121 gattgcacga ggagctggtg gcattgatcg aataagtgtg ccagtagacg gttcgtggaa
     6181 ttatgggcaa agtgattatg ctggcagtat tcttgaaatt gataatcaaa tgaatcaatc
     6241 tgctattaca gattttttga ataaatgatc ataaaaaaga tcaagaagaa gctgcgcaag
     6301 gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa acgctttctt atggtagatt
     6361 taaccaagag aggagctgtt tacattggat atctatctga acttcaaaaa tgagtcgcgt
     6421 gaagccatcg ctttttatga aaaagttttt cagactgaag catcaggtat caccacatac
     6481 ggtgaaatgc ctggaataga acaaatgaac ttgccagaag aagcaaaaaa tctaatcacg
     6541 aatgcacggc tgaacattta tgatacatta gttatgtttg cagatacgcc agaaggaatt
     6601 ggtcctgaat tagttgtggg aaataatgtt tctttagtca tccagacaag aaatgaagaa
     6661 gaaacgaagc gcctatttga aagcttagct gagggtggta agattgagat gccactggat
     6721 gctgttccgt gggctcagtt gtatgggatg gtaactgatc gatttggcat caactggcag
     6781 attaaccaag agtgacagct aaagatgaat attttctgga aacatcaagc agcaaacgga
     6841 tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac tatacggtat ctttttttta
     6901 ttgccagatg cgaaaatatg ttccataata gagaagtccg caaaaaatta agtaacggag
     6961 gtcagaagat gaagacggtg atactagctg aaaaaccttc acaagcaaaa gcctatgccg
     7021 aaaccttcca acagcaaaag cggcatgaag ggtattatga aatcagcgat ccattgtttg
     7081 ctggtgaagt aaccatcact tatggttttg gacatttggt agatatggtt gctccaggag
     7141 cttatgaaga gcgttgggca aaatggtctt tagaaaattt gcctattttc ccagaaacct
     7201 tccgttacga agttccaatg gacaaacgtg cacaattttc gatcgtaaaa cgagaattgc
     7261 agtcagccga taccatcatt attgcaacag atggtgatcg agaaggtgaa gccatcgcgt
     7321 ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa gaactattta cgtttatgga
     7381 tcaattctct tgaaaaagaa gcaatctatg aaggttttaa gaatctccaa cctggaaaga
     7441 actattttcc aaaatataaa gaagcgcaag caaggcagaa cgctgattgg ctgattggaa
     7501 tgaatggaag tccattatat agcttgctac ttcaacgaaa agggattgat ggcagttttt
     7561 ctcttggtcg tgtacaaaca ccgacactct atatgattta tcagctgcaa gaagaaatca
     7621 aacacttcaa aaaagaaacg tactttgaag gagaaggttt aattacgaca aacaaagggc
     7681 agttttctgg aaaaatcgta ccaaaaaaag catttaaaac ccaagaagag ttaacgaagg
     7741 aaatagaaaa acttggagcg catttaggta agcaaaacgg acggattctt gaagttacga
     7801 aaaaggaaaa gaggttgcat agtccacgtt tattttcgct atctagtttg cagacaaaga
     7861 tgaatcagct aatgaaagca agtgccaaag ctacacttga tgctgctcaa ggattgtacg
     7921 aaagcaagtt tctaagctat ccaagaacgg attcctttta cattacagaa aatgagcatc
     7981 agtatctggt taaacatctg caaaattata aaaaattttt aggaatagaa gaagtacaga
     8041 caactgagat caaagcaaag aaacgatatg tcgatgcgaa aaaagttcaa gaacatcatg
     8101 caattattcc gacaaagacg attccgactc ctgcacgctt tgcgaagctt tccaaattac
     8161 agcaagcaat ctatttgcaa gttttgcgca ctactgtggc tatgtttgct gcagattata
     8221 tctacgaaga gacgaccgta atgacgggcg tacagcaatt aaagctaaaa agcatcggaa
     8281 agatcccatt acagcaaggt tggcaggaaa tcttacgcat caagtcaaaa aagaaagaag
     8341 ctcagactct tcctcctatc gtagaaaatg aagaagcaga agtagaactg aaaagtatgg
     8401 agaaagaaac acaacctcca aaaccctata ccgaaggaac gttgatcaca gcaatgaaaa
     8461 ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat tctcaaagaa gtagaaggaa
     8521 tagggacaga agctacacga gccaatatca ttgaaaatct taagcagaag cattacatcg
     8581 aagtcaataa aaacgaaata actgttacgc caaaaggaat cactctttgt aaggcagtag
     8641 ccaatgaacc attactgacg agcgcagaaa tgactgcgcg ctgggaaggg tacttgagaa
     8701 aaattggtaa acaagagggg acaccatctg tttttttaga gaatatcaaa aaattcatcc
     8761 tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt agacttttca gatgagatta
     8821 gcgggacacg tcagataaaa gcgatggaga agaaaaacga tcaattagga agatgtccaa
     8881 aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc tacatgcttg aatccggaat
     8941 gcgacttcaa attgtggcca acagtagcta aaaagaagct gactaaaacg aatctcagag
     9001 atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg ttttactggt aaaaaaggga
     9061 aattcgatgc agttttagta ttaaaagaag atatgacaat cggtttttct tttccatgga
     9121 tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga aacatcaaga aaaaataatg
     9181 aaaacgaagt agagtaaatg aaaacaatga aatagcggaa tatttattcc gaaaagtgag
     9241 aatataaata ccaataaaac taaaaaatat cagtgaatca agcaaaaacg ttggggtagg
     9301 ctgattttat atagatagca aggagttttt ttatttgaaa aaagtgcatt catttttaaa
     9361 ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa gcatttctaa agtatcctga
     9421 ttacgggacg aagttgttgt cattgacgca caatcaaaca gcatattcac caacgttgat
     9481 tttatcgact ttgactgcag gagctaaaag cgctacttat ttttttcata aaacgacaag
     9541 catgctaaag cgaaagaaac agcgaaaaca attgttggtg gtggtagcgg ctatgatcat
     9601 tatttatttt ctaccagtta ttacacaagt atttcatcaa tattttggtt aagaatacga
     9661 gagtttgtga caaaagtcat gtcacaaact tttttc
//
LOCUS       DACESX010000096         9664 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.096, whole genome shotgun sequence.
ACCESSION   DACESX010000096 DACESX010000000
VERSION     DACESX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.096"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..650)
                     /locus_tag="IXF88_002022"
     CDS             complement(<1..650)
                     /locus_tag="IXF88_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1508744.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="IXF88_002023"
     CDS             975..1391
                     /locus_tag="IXF88_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1508745.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="IXF88_002024"
     CDS             1388..1858
                     /locus_tag="IXF88_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1508746.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="IXF88_002025"
     CDS             1872..2645
                     /locus_tag="IXF88_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1508747.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="IXF88_002026"
     CDS             2659..3471
                     /locus_tag="IXF88_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1508748.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="IXF88_002027"
     CDS             3525..4490
                     /locus_tag="IXF88_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1508749.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="IXF88_002028"
     CDS             4606..7329
                     /locus_tag="IXF88_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1508750.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="IXF88_002029"
     CDS             7342..7974
                     /locus_tag="IXF88_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1508751.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9664
                     /locus_tag="IXF88_002030"
     CDS             8873..>9664
                     /locus_tag="IXF88_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1508752.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3260 a         1477 c         1884 g         3043 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtat
//
LOCUS       DACESX010000097         9663 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.097, whole genome shotgun sequence.
ACCESSION   DACESX010000097 DACESX010000000
VERSION     DACESX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.097"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            34..771
                     /locus_tag="IXF88_002031"
     CDS             34..771
                     /locus_tag="IXF88_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508753.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            785..1600
                     /locus_tag="IXF88_002032"
     CDS             785..1600
                     /locus_tag="IXF88_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1508754.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1613..2260
                     /locus_tag="IXF88_002033"
     CDS             1613..2260
                     /locus_tag="IXF88_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1508755.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2276..2932
                     /locus_tag="IXF88_002034"
     CDS             2276..2932
                     /locus_tag="IXF88_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1508756.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3094..3915
                     /gene="racE"
                     /locus_tag="IXF88_002035"
     CDS             3094..3915
                     /gene="racE"
                     /locus_tag="IXF88_002035"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1508757.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3918..5273
                     /gene="rph"
                     /locus_tag="IXF88_002036"
     CDS             3918..5273
                     /gene="rph"
                     /locus_tag="IXF88_002036"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1508758.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5274..5792
                     /locus_tag="IXF88_002037"
     CDS             5274..5792
                     /locus_tag="IXF88_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1508759.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5899..6399
                     /locus_tag="IXF88_002038"
     CDS             5899..6399
                     /locus_tag="IXF88_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1508760.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6679..7599)
                     /locus_tag="IXF88_002039"
     CDS             complement(6679..7599)
                     /locus_tag="IXF88_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1508761.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7764..8777
                     /locus_tag="IXF88_002040"
     CDS             7764..8777
                     /locus_tag="IXF88_002040"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1508762.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8887..9642)
                     /gene="fabI"
                     /locus_tag="IXF88_002041"
     CDS             complement(8887..9642)
                     /gene="fabI"
                     /locus_tag="IXF88_002041"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1508763.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         3122 a         1700 c         2079 g         2762 t
ORIGIN      
        1 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
       61 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
      121 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
      181 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
      241 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
      301 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
      361 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
      421 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
      481 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
      541 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
      601 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
      661 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
      721 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
      781 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
      841 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
      901 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
      961 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
     1021 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
     1081 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
     1141 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
     1201 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
     1261 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
     1321 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
     1381 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
     1441 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
     1501 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
     1561 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
     1621 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
     1681 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
     1741 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
     1801 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
     1861 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
     1921 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
     1981 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
     2041 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
     2101 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
     2161 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
     2221 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
     2281 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
     2341 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
     2401 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
     2461 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
     2521 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
     2581 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
     2641 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
     2701 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
     2761 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
     2821 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
     2881 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
     2941 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
     3001 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
     3061 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
     3121 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
     3181 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
     3241 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
     3301 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
     3361 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
     3421 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
     3481 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
     3541 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
     3601 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
     3661 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
     3721 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
     3781 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
     3841 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
     3901 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
     3961 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
     4021 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
     4081 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
     4141 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
     4201 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
     4261 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
     4321 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
     4381 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
     4441 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
     4501 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
     4561 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
     4621 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
     4681 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
     4741 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
     4801 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
     4861 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
     4921 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
     4981 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
     5041 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
     5101 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
     5161 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
     5221 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
     5281 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
     5341 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
     5401 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
     5461 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
     5521 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
     5581 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
     5641 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
     5701 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
     5761 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
     5821 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
     5881 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
     5941 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
     6001 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
     6061 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
     6121 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
     6181 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
     6241 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
     6301 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
     6361 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
     6421 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
     6481 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
     6541 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
     6601 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
     6661 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
     6721 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
     6781 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
     6841 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
     6901 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
     6961 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
     7021 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
     7081 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
     7141 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
     7201 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
     7261 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
     7321 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
     7381 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
     7441 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
     7501 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
     7561 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
     7621 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
     7681 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
     7741 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
     7801 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
     7861 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
     7921 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
     7981 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
     8041 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
     8101 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
     8161 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
     8221 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
     8281 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
     8341 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
     8401 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
     8461 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
     8521 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
     8581 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
     8641 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
     8701 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
     8761 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
     8821 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
     8881 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
     8941 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
     9001 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
     9061 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
     9121 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
     9181 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
     9241 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
     9301 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
     9361 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
     9421 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
     9481 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
     9541 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
     9601 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
     9661 ctg
//
LOCUS       DACESX010000098         9596 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.098, whole genome shotgun sequence.
ACCESSION   DACESX010000098 DACESX010000000
VERSION     DACESX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.098"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            77..352
                     /locus_tag="IXF88_002042"
     CDS             77..352
                     /locus_tag="IXF88_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1508764.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            587..1633
                     /locus_tag="IXF88_002043"
     CDS             587..1633
                     /locus_tag="IXF88_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508765.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            1635..2894
                     /locus_tag="IXF88_002044"
     CDS             1635..2894
                     /locus_tag="IXF88_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1508766.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            2900..3481
                     /locus_tag="IXF88_002045"
     CDS             2900..3481
                     /locus_tag="IXF88_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1508767.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(3563..4438)
                     /locus_tag="IXF88_002046"
     CDS             complement(3563..4438)
                     /locus_tag="IXF88_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1508768.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            4586..4918
                     /locus_tag="IXF88_002047"
     CDS             4586..4918
                     /locus_tag="IXF88_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1508769.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            5030..6238
                     /locus_tag="IXF88_002048"
     CDS             5030..6238
                     /locus_tag="IXF88_002048"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1508770.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            6392..6847
                     /locus_tag="IXF88_002049"
     CDS             6392..6847
                     /locus_tag="IXF88_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508771.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            7209..7484
                     /locus_tag="IXF88_002050"
     CDS             7209..7484
                     /locus_tag="IXF88_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508772.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            7497..9575
                     /locus_tag="IXF88_002051"
     CDS             7497..9575
                     /locus_tag="IXF88_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1508773.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
BASE COUNT         3205 a         1601 c         2000 g         2790 t
ORIGIN      
        1 aacttctgga ccactcagaa agttttaact aaatgatatt tctatcatat ctcttgtgga
       61 ggaggtgaat ctatccatgg caaacaaagc agaattaatc gaaaaagttg cttcagctac
      121 tgatttaact aaaaaagatg caactgcagc agtagatgct gtattttcaa ctattcaaga
      181 tgctttagct aacggcgaaa aagttcaatt aatcggtttt ggtaactttg aagttcgcga
      241 acgcgcagct cgcaaaggtc gtaacccaca aactggtgaa gaaatcgaaa ttccagctag
      301 caaagtacct gcattcaaac caggtaaagc attaaaagac gctgttaaat aagacagttg
      361 aaaaagcgct tgtggctcta gggctacaag tgcttttctt tttatttcct agcagaaaag
      421 gaataaataa gagacagaga ttccgaatcc gtatgtcata catagaaaac acatgtattt
      481 tatttaattt ttatttattt gcaagttatg aggtgagctt tgctcttatc ttttctagaa
      541 tttgcttact aatggtaaaa taaaaataag aagaggaggg atgcgtatgg aacttgatta
      601 tcaagtagaa gatgcgttga cgaaagtgtt ttacggagaa acgctagtca gtcagctaag
      661 agaactttcc attgatcagc caatcttatt tttaacgaat cagcgctatt atgatttgtt
      721 tgcagataag atcaacccgt tatttgcaaa tcaagcaaac aatgactggt atatttgtgc
      781 caacactcaa tgcaatcatt taacagaatt aaagaacctg cttgatttcg tcaaacgcta
      841 tcccgaaaat caatccatgt ttatcatcgg atttggaaat gaagggatca tggatctggc
      901 aggttttttt caaaaacata caacccttga ttcatcacta tggatgatcc ctgtatcgat
      961 ccgttctcta agccgagcat taacaccgca gcggacgatt ttgcagctac caaatcgtgt
     1021 agtgctgcaa accatcaatc tgccagaacg catcgtttat gatcaaacaa tcagcgagcg
     1081 gcaggtggac ggaaagcaga ttgacttttt gacttttatc tgttgcggaa tgatttgtga
     1141 tcatcgtttt ttgcaaaatc tgtataaaaa ctatccaaat aaaaagcaat taacgactcg
     1201 gccgtttgcc gggatgatcg aagaaatgat ctatttttat caattagagg cagaaaacct
     1261 gtcaaattat ggtaaactgt ttgaacaagc tttttatctg accgaaaatg gccatctact
     1321 ttctgctagt atgaaaaaat ttttaggaat gatgtttcat ttcgtgtgga atctccaagc
     1381 atatcctttt gatttccaac taaaaaactt ttttgtgtgg ctggacttgc ttggctatcc
     1441 aatccactgg ctggaacaga tttcaaagat ggagtatttg gaaaaagttc tttttttggc
     1501 agaaaaaagc aaaaaaatac ttgttttaga aaaaataggt ataatagacg gataccaaca
     1561 tccaaacgaa aaagaattat tgagaacaat gacggtattt caacaaatcg tcagtgagat
     1621 aagagggaat tgaaatgaat acaaatagcg aaaaaatgtt gcaagcttta tcagatgaag
     1681 acttgtcaca agcacaaatc tttttagaaa aagcattaaa agaagaccca gcagatatat
     1741 tagctgaact aggcgaagaa ttgctggcta ttggcttttt gatcgaagca aaacagattt
     1801 ttgaacaact gatcaaccaa tacccagaaa atgacggact gaacatcccg ttagcagaaa
     1861 ttgcaatcga agataatgag atcgacttag cttttgaata cctggaaaaa gtggataagc
     1921 aaagtgatta ctatccgcaa agcttattgg cgatagctga tttgtaccaa gttatcggaa
     1981 tccctgaagt gagtgaagca aaattgaaag aagcagctgg gttgcttcct gatgaaccat
     2041 tgatccagtt tgcgctagct gaattgtatt tttcagttga tcgtttcaaa gaggcagcag
     2101 tggtttatga aacattgttg gcaagcggga taaatgaaat ttcaggaatc tcgatgcagg
     2161 aacgtttagg acaatcctta agtatgcaag gagaattcga agcagctatc gtacctttag
     2221 aagaagcact gaaagaagaa aggacagatg atcgactgtt ccagctagct tttacgaatc
     2281 ttcaattgaa agaaaatcag caggcaatca actatctgga agaacttcga gaagttaatc
     2341 cgcattatca atcgttatat ctatatctag gacaagctct tcaagaagaa gaaatgattg
     2401 aagaagcaca aacagtgtta gaagaaggaa tcaaagaaaa tccttaccaa gtcgaacttt
     2461 atcacctggc ctcggaaaat gctttccgtc tgcatgatag acagcgagca gaagaacttt
     2521 tactgaaagc tttagaagta ggagacaaac aagatgaaac attacttact ttaagtaatc
     2581 tttacttgga tgatgaaaga tatgaagatg tgatacaagt gatcaatcag atggaagaaa
     2641 ctgctaatcc ttatgcagaa tggaacctag cccatgctta taatgaatta gaagacttta
     2701 ccgttgcagc tgtacactat gaacaggctt atcatgaact atcacatgaa ccagatttct
     2761 tgaaagagta tgcctttttc ttaagagaag aagggcagct gaaaagagcg caagaattat
     2821 tgacttatta tttaacactt gaaccaggag atatggaggc actgtcatta ttagatgacc
     2881 taacagagag gtgagaaaaa tgctaattga tgtacgggaa aagaagaatt ttttaacgtg
     2941 gatggtcaat catgtatctt ttagccgaag agaagttttt tggatcttga attatcttgc
     3001 caatcatgaa gcgattttaa gcaatgtcca ctttgttgaa ggggcgaatt ttgcggatcg
     3061 aggactgcag atcagtgatc tatctgttga aggggaaccc atgaaattgt ttttgcaggg
     3121 aaaagaattt acggacacag accagatttt tcatgaaatc cgtttgaatt ggaaaaagcc
     3181 tttatatgtg gaatgtatat tcgaaaatgc ttggcagaca agagaatatc tatctatctt
     3241 agaagacaat ccattttctt cttgggattc ttcagtcagt gaagaagata ctgaattaat
     3301 tgaacgatat tttcaagaaa aagaagaaga ggcaaaacta aaaatgcttt atgctcaaat
     3361 cgatcaagca ctagaagatg gagataggga tgcttttttg aaattatctg atgaagttaa
     3421 ccgaaagatc cttttgcaga cacaaaagaa aagtaaccgg gtaagtgaac ataacaatta
     3481 gttaaaaata gtatagtact aaaaaagaca aaggtcagct ggatgtggaa tctttcctca
     3541 tagcctatct ttgtcttttt tcttatagtt ttttcagtaa tcttgtctgt ggcttcaaat
     3601 aagtaaagcc ttgcccttcc acttcatgca cattgatgat tgaaaggaaa gctttttcgt
     3661 caatctcgtg gacgatcctc ttagcttcag ttaattcact agagctaacg accatataga
     3721 tgattttttt tgaattacca gagaatgcgc cttctccaga aaggaaagtc acacctcttt
     3781 gcagaccggt catcagtagt ggtgcaatct cttcgctttt gttggagacg acaagtagac
     3841 cttttgcaga atatccgcca tctaagatcg aatcaatcac acgactgaaa acatacgaaa
     3901 caatcaacgt atacatcata cgtttcaagt cgatataggt taatgaagca agtaagacga
     3961 tgatatcaaa tgccaatagg gaacgcccca tagtgatgcc gtaattttgt tctaaaatcc
     4021 gagcgataat atcactgccg cctgttgttc cacccatgcg gtagacgatc ccgcttccta
     4081 cacctgcaac taaacccgca agtagagaaa cgatcagtag atcatgctgt aaattgattt
     4141 ctagtggaac cctttgccag atccataaaa agaatgacaa agaaagtgta cctagaacag
     4201 tataggccaa ggaacgttta ccgaggatct ttcctcctag taaaatcaat gggatgttga
     4261 ttaataaagt cgtataagct ggatcaatac caaataaagc tcttaagatc aaggtgatcc
     4321 ctgagactcc cccttctgct aaatgattag cgatgttcaa gtaaacaaga ccaaatgcat
     4381 aaatacttgt tcctataatg ataaaaagaa cttcttttac tgtttttcgt ttgaccatat
     4441 gaattccagc tttcaattat gtattttcac agaatttcca tttagaaatt ccttcctaaa
     4501 tgtagcatgg aaattcgatt ttctcaattg ctcttatcta ggatttgcgt taaagttttg
     4561 gtaagatagt tttggaggga aaaagatgga taaaaagcgt tctttataca gtatgcagca
     4621 ggaagtggat gaatatatcc agcaattcaa ggtggggtat ttctctccat tagctcagat
     4681 ggctcgatta actgaagaag taggtgaatt ggcaagagaa gtcaaccaca cttatggaga
     4741 aaaaagcaaa aaggcttctg aaccagttaa ctcggtagca gaagaacttg gtgatgtttt
     4801 attcgttacg atgatcatgg ctaattcatt gaatatcgac ttaactgaag tttttgaaaa
     4861 aaacatggaa aaattcaatc aaagagacca taatcgcttt gagcgtaagg acgagtaaac
     4921 aagatgaagt ggtcctggcc ttgaagactc tgaaacggac ttgtcaaaca gtcaccatgc
     4981 aactgagtct ttatctcagg acaagaaaca cgcaggaaag gttggaaaaa tgaaattaaa
     5041 acaattacct ctggaatacc aaaaggcaat tccagttctg aaaaaattag agaatgcagg
     5101 atatgaagct tattttgtag gtggaagtgt tcgtgatatc ttgctgaacc agccaatcca
     5161 tgatgtggat atcgcaacaa gcgcatttcc agaagaaatc aaacagcttt ttccaaaaac
     5221 gatagatatt gggatcgaac atggaactgt gttggtatta gaaaatgatg aacaatatga
     5281 gatcaccaca tttcggacgg aatcaactta tcaggactac cgtagaccag atcatgtcga
     5341 attcgttcgt tcgttggcag aagatttgaa aagaagagat tttacgatca atgcgtttgc
     5401 tttgaaagaa gatggagaaa tcatcgattt gttcgatggt ctgcaagatc tagaagaaca
     5461 gatcttgcga gctgtaggca atcctcatga gcggttccat gaagatgcct tacgaatgat
     5521 gcgcgggttg cgttttgtca gtcaactagg gttcaaatta gaggaagaaa ccttctacgc
     5581 catcaaagaa aaccacgagc tattaggtaa aatatctgta gaacgtataa atgtggaatt
     5641 catcaagatg ctcttgggaa atgaacgggc cgcgggaatc catgcattta ttgaaacaga
     5701 gtgctacatt tgctgtcccg gtttgaaaga agaaggcgaa gcacttttac gttttgctga
     5761 attaccatcc gtcccattga aagaagagag ccaagcatgg gcattattga tcagccaact
     5821 ggatctaaaa gaaaatgaga ttcgttcctt tttgaaatcg tggaagtgtt ctaaccaaat
     5881 gattaaagac gttcaagcac ttgtccaagg actacgacaa cgagaaactg gagctttgga
     5941 accattcgaa ttgtacttct taggcaaaaa acatgcactg caagtagaag aattgatgat
     6001 ctattttgac gaaacacctc agatggatgc cgtaggagaa tcctatgatc gtttacctat
     6061 caagtcatta agcgaattag cagtagatgg taaaatcttg ctagaagaaa ctggaaaaaa
     6121 acctggtaag tgggttagtg aagctttgca attagctgaa aaagccgtcg tagaacaaag
     6181 aataaaaaat gataaaaaac aggttttgga ttttttatcg aaagaaaaat tgatttaaaa
     6241 aacactagac tgtatctgct cagctttgtt ggctacttaa agtgggcaga tacgtcatta
     6301 gaagaaaaaa cataagatgt gaccacttac ataaatttgt gctaaactga ggtagtacaa
     6361 gaataaatta catcataaag gagtcttttt aatgagtaaa gaaatgacag cgctaaaatt
     6421 ttattttaga aatggagaaa cctggacgat caaccgccgt catatcggcg atctttggat
     6481 caaacaaatc acaacaagtt ttggacgaat caatggcagt gaatttgttg aaatccatcc
     6541 gtgtgctgga tttaaaatcg aaatcttcca tgaaggggac gcagttgcga cacatgatat
     6601 caacctaggc ggactggaaa tgggcatgtt taatcgcgca ttgaaatacg aagatatcga
     6661 gcgaatggaa atcctttacc gaaatggcac acctgatctt gtgtacttcc catatctaga
     6721 taaaggaaca gaaggattag acaatcaata tcaatcaaca aaaatcagcg aaaaaacagg
     6781 taacttgtat atcgtgatca atccagatca acgtgtagaa gatgtatacg gggaattttt
     6841 cgaataaatc gatttttacg caaaaatgag gctgtgaaag agatgtgtct tattactact
     6901 gataggctgt ctcttccaca ccgtttattc agaataaggg actgtgacaa gatttttgtc
     6961 gcagccccat ttttttgttg tgataaaaga acaagaaagg caggaatggg aaagtaaaca
     7021 tccttttgtc agaaactttt atctttctta agttttactt gatatctgat actatttgga
     7081 atagaatgag gcgttgaaaa atttatggaa atttctaact agcaataaac gatgaaacaa
     7141 tgaaatattt ttcatccaaa aagaactttg ctataatgat ggaagtttgt gtataggaga
     7201 tctggaaaat gagagagagt tttgaacaac aaaaaaaact gttgcatgat cgctatggcg
     7261 ccctttcgat ggatgaccgc cgtcaaatac tttgtaagtt gcgtaaaaga aatatattga
     7321 tgtatcgtca gttggaacgt ctgaaacatg atctgcttcg tttggagtca aaacgtgtac
     7381 agtgtgaatt agaggggaac caaacacagg ttgaagtagt cgaaaccaaa attttaaaga
     7441 aaaaagaaca atttttaaaa atgcttacac aaaataaaaa ataaagggga tggacgatgg
     7501 aatttgctga gttagttatt gtatttgcag taacgatcac cttatcgaac atagctagcc
     7561 gcattttgcc aatgattcca gctccactga tccaaatttt ccttggagtg gtattaggtc
     7621 tgacagagtg gggacaatca atcgattttg aacctgaatt gttcttagtt atgattattg
     7681 caccattgtt atttagagaa ggagaaaaag cagatatttc ttcgattctc aaaaatttcg
     7741 ggacaatttt gttcttggca tttggaggag tcattttgac ccttgtcggg gtaggtgcga
     7801 cgttatcatt cctgttgcca agtgtcccac ttgctgcctg ctttgctttt ggagcagcgt
     7861 taggtccgac agatgcggta gcagtcagtt ctctttctgg acgagtgaat atccctaaaa
     7921 aggcgatgca tattttggaa ggagaaggtc ttttgaatga tgcatctggt gttactgcct
     7981 tccagtttgc tcttggtgcg ctgatcaccg gatctttttc agcagtaaac gcggggatga
     8041 gtctagtagt ttctagtatt gggggagctt tgatcggctt tttgcttgtc tggttcaaac
     8101 aaaaaataat tcatttgatc gaaaaagcat cagcacaaga tgtcacaggg tatctgctga
     8161 tcgaactttt gcttcctttt ctagcctatg tattggcaga ggcatttgat gtatctggaa
     8221 tcattgctgc agtggcagca ggtattctcc aagcttctgg ttttaggaaa atctctgtgt
     8281 ttgatgcaga attgtcgagc ttgtcccaca gtacatggac aactattgct tttacactga
     8341 atgcattagt atttatattt ttaggaatcg aattgacgca agtgttctcg ccagtatggg
     8401 gagacggttt atatccaaac ggtcttttat tagcgattat cgtattaatc tctgtcatgc
     8461 tttttgtgat cagattcatt tctatcagtc ttttttatgt attcaaagat ggcagtaaaa
     8521 agttcaaaaa acagttgaac gagatcttga tcttaacatt tggcggtgta aagggaacag
     8581 tcagtttggc tacgattttt attttgccat tttcaatcaa taatatgatg ttttaccagc
     8641 ggtctttgct tttgtttctg acggcgggtg tcatcttagt gactttggtc atagggatca
     8701 ttgtcttgcc tatgctgacg gaaacagagg aagcgaaaag cacggatttg aatgctttga
     8761 tgattttaga agaggtcgtt gaagttttgc gaaaagaaat aaaggaaatt gatcgtaata
     8821 caaaagaatt tttagccact gaagcagtga tagaaaatta tcaagaacgg atacgtgatc
     8881 tgtacttgga agatttaacc gatgatgaaa agcaagaagt acaagaaata caagcactga
     8941 ttttgtcgat cgaacgggat ggattggatg aaagttatcg ttcgggcaag ctttcttcta
     9001 atggttatcg tttttactcc cgttttcttt ctcgattcga acattctatc acaagccaga
     9061 ttttgtcttt tatcggcttt tggttcattg ttgtccgtag gttaatgcga atcgttctcc
     9121 atccgaaaat gttttggcaa agaagatatg ctgatcgtca gacatttatt agtgatgaag
     9181 atatccaaga aatcagagag acatatcaaa aaaatactca gttgattatt gaaagtttag
     9241 ataacttgac tgatatttat gatgaaacga tggttcaatt ttttatccag caacgcaaac
     9301 ttgaaggaat aaaaatgaac tcggggaatt tgatttcttc gtggatgatc cagcaagata
     9361 gtctgtttac gaaaaaaatg ctaagaggtt attatcttga aagaaaaatc attgatgaat
     9421 acgaagttgc tgaaaaaatc acgacgtttt ctgcgaacag ctatcgcagg aatatcaatt
     9481 tgttagaatc ttatacgatg aacaaaccat ctgataactt ttctttccga tttgcgtttc
     9541 gagcaaagtc aaaaaaaacg agtccaataa aataaaaagg taaaaatttt caagtt
//
LOCUS       DACESX010000099         9411 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.099, whole genome shotgun sequence.
ACCESSION   DACESX010000099 DACESX010000000
VERSION     DACESX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.099"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(142..810)
                     /locus_tag="IXF88_002052"
     CDS             complement(142..810)
                     /locus_tag="IXF88_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1508774.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            944..1231
                     /locus_tag="IXF88_002053"
     CDS             944..1231
                     /locus_tag="IXF88_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508775.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1647..3185
                     /locus_tag="IXF88_002054"
     CDS             1647..3185
                     /locus_tag="IXF88_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1508776.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3182..4147
                     /locus_tag="IXF88_002055"
     CDS             3182..4147
                     /locus_tag="IXF88_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508777.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4134..5456
                     /locus_tag="IXF88_002056"
     CDS             4134..5456
                     /locus_tag="IXF88_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1508778.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5519..5623
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5697..6440
                     /locus_tag="IXF88_002057"
     CDS             5697..6440
                     /locus_tag="IXF88_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508779.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6455..7447
                     /locus_tag="IXF88_002058"
     CDS             6455..7447
                     /locus_tag="IXF88_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1508780.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7459..8196
                     /locus_tag="IXF88_002059"
     CDS             7459..8196
                     /locus_tag="IXF88_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508781.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8186..8908
                     /locus_tag="IXF88_002060"
     CDS             8186..8908
                     /locus_tag="IXF88_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1508782.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8992..>9411)
                     /locus_tag="IXF88_002061"
     CDS             complement(8992..>9411)
                     /locus_tag="IXF88_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1508783.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3093 a         1614 c         1976 g         2728 t
ORIGIN      
        1 caaaataaaa aaacaaacta ttttcgcaga tcgtttgcaa aaatagttta tttttttata
       61 aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct gtaaattaaa
      121 taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa gtggttcaaa
      181 tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt ccagatactt
      241 cactttattc aatgtataag atagaataga acagctaagt aatgtataca gaaaatgtgt
      301 gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga aaagactttt
      361 gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg ttccgcctgt
      421 gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac ctgtcatcaa
      481 actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc ataaatgtaa
      541 aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt ttccaaaaag
      601 aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag aaggagcgaa
      661 tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg ccagcttaga
      721 tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa tgattccgca
      781 caattctgta aataaattta attttttcat ttctagcctc ttttcttttg aaaattttca
      841 gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga aaaaaaatta
      901 tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag cagcagactt
      961 attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt ttttggctca
     1021 agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc aatttatctt
     1081 tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt ttttgatgaa
     1141 gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc tttatggagt
     1201 gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag acgtttatga
     1261 tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca gtataatatt
     1321 cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat tggctgtttc
     1381 tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca agtgacaatt
     1441 tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt tgatccgact
     1501 ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct tttttttgtt
     1561 cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa tgtttatact
     1621 taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta agtgaaagaa
     1681 aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt tctttgctga
     1741 ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa aaaatggcag
     1801 aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc ttattaggtt
     1861 tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt gttatttacg
     1921 ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac ccgtctattg
     1981 aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa gtagagcttc
     2041 ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta atcatcggta
     2101 cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt ttgttaggta
     2161 ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc ttgcttgcaa
     2221 ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat tccatcatta
     2281 ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct gccttgttta
     2341 aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc ctgatccaaa
     2401 tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa ccgaattttg
     2461 gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc tatcctgagt
     2521 tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa agtgctgatt
     2581 tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa ttcgatatga
     2641 cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag agatcttttg
     2701 cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat cagatcaaag
     2761 cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc caagaagcat
     2821 atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac caagcaatca
     2881 ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc aaggagtacg
     2941 atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc gcaccgctga
     3001 tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat aaattaagcg
     3061 gaaaaataac agaggatcaa atgattgata tgaattacca agtcaatgtc gaaaagaaaa
     3121 agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag gaggaagatc
     3181 agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa agtatgtcat
     3241 cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg gtccttccgg
     3301 tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat cagcaggaga
     3361 catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc ggtggaatat
     3421 gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc aaaatattga
     3481 agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg tagatgaact
     3541 tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta gagagttgtc
     3601 tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc cagatgtgat
     3661 tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat tacaagaatt
     3721 ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc acaatatgga
     3781 agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa ttatccaatg
     3841 tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc atttctttga
     3901 tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg gatatttctt
     3961 aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta tgaaagaagt
     4021 atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa gaagtattgg
     4081 gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag gaaatgaaaa
     4141 catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct tatggattga
     4201 aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg acttgtccta
     4261 accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt aatcgggtaa
     4321 aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa gaacttcaaa
     4381 aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa ttagcagaag
     4441 ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct attgaagtca
     4501 acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga cctacgatct
     4561 taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca ttgcctgtac
     4621 tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta gctacgattg
     4681 ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca ttaaaagaat
     4741 ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc attcagtttg
     4801 cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt ttagtcggaa
     4861 aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag ccgaatacag
     4921 aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt gcagtcaatg
     4981 attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt tcaaggaaaa
     5041 ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag cggataacgg
     5101 atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac gccagtccta
     5161 aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa gtggtagaga
     5221 tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct aaggctgaat
     5281 tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag caagcagacg
     5341 aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa gagttggata
     5401 gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg aaataatcag
     5461 catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa aaaatgaata
     5521 cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag acccattgaa
     5581 cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg ggttactatg
     5641 tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt aacaaaatga
     5701 agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg cagattgttg
     5761 tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta ttttctgcat
     5821 tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat gaagcagtgc
     5881 ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg gattattgga
     5941 agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg ccaatcatgg
     6001 tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa gtgatccttg
     6061 ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg aggaaagtat
     6121 caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag atttatcgaa
     6181 tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg gctgctactt
     6241 attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc ttagggtatt
     6301 acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg atcatttgtg
     6361 tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa gggtattatc
     6421 gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc ttttattacc
     6481 gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca aacaagaaat
     6541 aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta atcatactgg
     6601 catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg tccaaatcat
     6661 cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag cacaatttgg
     6721 tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata taccagtcaa
     6781 agcaattgct acagtgatca aacataatac ttctggattc gccaccctat cagacagtaa
     6841 catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa gtccaagtga
     6901 agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa aactgacaat
     6961 ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata tccaatggta
     7021 ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa actatatccc
     7081 attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta cgaatgatca
     7141 actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga aaaaaggcta
     7201 tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat atgcaaaaga
     7261 aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt atacggatga
     7321 tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag cttttatgca
     7381 ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta atcagtttat
     7441 aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt cattgaaaac
     7501 ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac atcgattgtc
     7561 gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt ccagcctatg
     7621 acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac agcatttagt
     7681 tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa cgttacgtta
     7741 tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta tctggatatc
     7801 tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg gatgaagcag
     7861 cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct tgatgaacca
     7921 tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt gaagctgcct
     7981 aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc aatttattta
     8041 tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga gattcaagtg
     8101 cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa gcaaacgatt
     8161 tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt tagatgggag
     8221 tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca attgtgattc
     8281 gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag ccttaagtta
     8341 cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc cttttttgaa
     8401 aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg taccgatgac
     8461 tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg agaaacctgt
     8521 tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag cttaccctaa
     8581 tcactatttg atccattggt attcttcttt ggactatcaa caagactata ttgatttagg
     8641 ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc aactagctaa
     8701 agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag cagagtgggc
     8761 tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac agttaaagac
     8821 aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt atcaaaactt
     8881 ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag tgtcgcacct
     8941 ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat cttagtacca
     9001 accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg aagtaacata
     9061 ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt aagaagaatc
     9121 taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc ttgattcttt
     9181 ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag tttctgttac
     9241 agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt gaaccgtttc
     9301 ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt gaggttcagc
     9361 agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt a
//
LOCUS       DACESX010000100         9406 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.100, whole genome shotgun sequence.
ACCESSION   DACESX010000100 DACESX010000000
VERSION     DACESX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.100"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(193..999)
                     /locus_tag="IXF88_002062"
     CDS             complement(193..999)
                     /locus_tag="IXF88_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508784.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            complement(1010..1654)
                     /locus_tag="IXF88_002063"
     CDS             complement(1010..1654)
                     /locus_tag="IXF88_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508785.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKVVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            complement(1670..2869)
                     /locus_tag="IXF88_002064"
     CDS             complement(1670..2869)
                     /locus_tag="IXF88_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1508786.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            complement(2869..4893)
                     /locus_tag="IXF88_002065"
     CDS             complement(2869..4893)
                     /locus_tag="IXF88_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1508787.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            complement(5056..5649)
                     /locus_tag="IXF88_002066"
     CDS             complement(5056..5649)
                     /locus_tag="IXF88_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508788.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(5650..5982)
                     /locus_tag="IXF88_002067"
     CDS             complement(5650..5982)
                     /locus_tag="IXF88_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508789.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(6032..8565)
                     /locus_tag="IXF88_002068"
                     /pseudo
     CDS             complement(6032..8565)
                     /locus_tag="IXF88_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8562..>9406)
                     /locus_tag="IXF88_002069"
     CDS             complement(8562..>9406)
                     /locus_tag="IXF88_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAQ1508790.1"
                     /translation="CHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLWDLFIQSYQQ
                     LYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENSTINSLGMNTH
                     INRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHGETNLPYAYQ
                     YIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGLHDQLEEKNEE
                     NMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMIGKRLLLESEK
                     VVKE"
BASE COUNT         2523 a         1985 c         1487 g         3411 t
ORIGIN      
        1 gaattttaaa gtatctaaat tctttcgacc attcttcaac tcagatattg ttgtattaga
       61 taaattcact tctcgacata tttctttagc ttttacaact ttgagatagg ctttaatcaa
      121 tactttctgc tcagcgaaag tatctttgtc agccaagttt gttcgcttca ttttttcact
      181 ccacctgtcc ttctaaatct tttcttttgt catacgatca attttttctt gaagtttttg
      241 aatgatctct tcttgatctt tcatttgcgt tttcgatgtt tcaatttctt gctggtagct
      301 attaatcttg ttttcagtat tttcctgatc atctgcgact tgataaggca attgatcttt
      361 tagcttcttc actaattctt cattcaaatc aatggttgct tggaaggatt ccatttgttt
      421 ttgtgctgct ttttgttctt ttttgtatcc tttcactttg taattgatcg catgttgttc
      481 ataatcttca tctttcactt gatctttaac cttatcttcg tgaacgtaat aaatcagatc
      541 ttgtggttct tgcatatcca cttttggatc aattttttca ggaataatgg ttaaacgcat
      601 ggcgatatat tcttctggta catgtgctaa cttcactaca aaataatcat cagacacctt
      661 gatcactttg gcttttattg cttgataatc ccctttagct gtctttactt ctacacgata
      721 attaagatta gctagttctt ttaataagct ctgatcttca tcgtcgatcg caaattcaaa
      781 aactaattgc tggttcgctc ctttttcttt tgaaaccatc gtcagttcct tcgtgtttaa
      841 aaatgcgttc gtgcttactt catctggtat gtctaccttt ttatgtgtgt aaatcggact
      901 agcaagaatc aaaaagccga gaatcataaa gatccccagc catctaagat aactttttcg
      961 ttttttaaaa cgttgtcttg ctcctttttt cttactcatt ttcttcctcc taacgagtga
     1021 aataactaaa gtctgtgact ttccattgac catctacttg tttttcagtg acagttaatc
     1081 caccttttat tttttgtggt ttgacaactt taaattgagg attgccgatt gtataatcaa
     1141 caaatacgac aaaattttgt tcctctttta cactgcttgt ttgagcgtaa gtttgcacgt
     1201 cacctaatgt aaaagagtaa cttccgtagt cgtcatgttg gtgatcatcg tctgcaggac
     1261 ttaatttgtc gatcacttct tgggttgcga catcttttag tttttccgtt aattcatttc
     1321 tgcttaatga tttatcaatg gccaattgac cgaactcttc tgccgtcttt ctttgtgctt
     1381 cagttacttc ctcttttgct tttgtgccat cttgttgctt cagctgatcg atttcttggt
     1441 ttttctcatt gatggtttga tttaacttgt cgatcttcgc ctgtgcctca gaagaatttt
     1501 ttttgagctt atcagacgcg tttttagcca cttcttcaat tttataggta tttgtctctt
     1561 gttcttcttt aagctgccaa aacaacgcaa tattgccgat taaactaacg ctaattgcag
     1621 ctgttaagac gatttttaag gttttatttt ccatattttt tctcctgatc tattttttgc
     1681 tgctattcaa aaatggtaac ggatcgatcc atgtaccatc atcaatgaat gcatgtcctt
     1741 gtgcttgacg ccaatccatt cgagtaaatc ctaaatgcaa gtgtgctgta tctcgtatcc
     1801 caattacttg accaactttc acttgatcgc ctactttcac tcgagaattt cctgtactat
     1861 ttgcaaattc ttgatacacc atattcaagc catcgtaatt aatcacgatc acacacgcac
     1921 ccaaacctga aataccagga ttgccaacgt acacgacttt tccgccatga accgcatgaa
     1981 tctcacttcc tggatgatcg actgaaccaa agtctagtcc atcatggaac ccgttaggtc
     2041 ggaattctcc tcctggattt ttcccaaaaa gttgtcctcc actgaacgaa tttttatcta
     2101 agctacttcc tgggaatgga ttgccccagc ttcctgtaat cgtaccacca gttccgccgc
     2161 cttctctcca gtgtttaatc gcaaactcat cgtactccat caatttattt gtattaataa
     2221 tagacataca atttttataa taggcactgt cttcagtaat tcctgcggcg gcataccctc
     2281 ctcgtcctaa gatcaataag cttgcgccag ggcttttatt tccgacacaa gcattaaaat
     2341 ttgattgtgt ggctaccata aagccgtaat aggagaaggc agcttttgag ttgggaaact
     2401 tcatgtacca gccaccttca atgccaccaa ttccacgcgc agttccttta ggaaacagac
     2461 acccatcaaa ccacgtaatc ccaaaataat tgagatcatt tcttgcggac gccgacgtac
     2521 caaacgctga ttcataaccg tattgcgaaa ggatcacact tggtaataat ttatacttca
     2581 ctgctgcacc tacagcatat tgcgcaatct catcagaaaa agggcctctt cctttgattt
     2641 ctttataaat gggtaagcct tccgaatatt ctcctgtaaa agcttcgcca ccactacttg
     2701 aatcagaatt atcaatagaa cctgccgcaa gtattagaaa aagggtcaca aacacagtga
     2761 tcaatatagt aatcaaacct gcagaagtac caaacaccca caaaaataag cgccgctttg
     2821 cacgacgctt aacatactgt ttgattaaac tttcattttg gttttccatt attttcctcc
     2881 atcaaacaat tttaattgac gtttgttcgg actaaaatga acacgaatgg tttcttttga
     2941 gccaaaattg atcacagcgt ctccttgatt ttgttgagga atggattcta attctgagtt
     3001 cgtcattgat ttacccacta atccttctat atgacgtaaa atagattcat ccatacgcat
     3061 aaagatcttc gtagaagaaa attcaaagac tttcttcagt ttcgagaagt tatcagaaga
     3121 catattttct ggcaccattt cttgtgggga ttgtgtagct aaaacagtca ttgcttctac
     3181 tttacgcatt tctcgttgaa agtttgtgac ataatttacc gtttcaataa aatctggatt
     3241 aatcgcatta tggcactcat caatcatcgc caagaagcgt ttgaagtctt gttcttccat
     3301 ttgtttttct ttaattaaac gtcgttgtct tcttccttga atcaatgcct gattccaaat
     3361 caacgtcata gccatgtaca tttgacattg ttgtacccct cgatctaagc ctgtaataga
     3421 gtcaatgtca aaaaagacca actgttgttg gtcaaggtgt cgaatcgtac tatgcccatc
     3481 aaaaatcatg ccatacgtct cacataagga ttctacgatg gattccattt cttcttttcg
     3541 attctttttc tcttgggaag cacctggtaa ttccagttgt cctaaaaagt ctaaaaattc
     3601 tttgaacgtc ggataagctt ctggatcttt acctgtgact tgcgtctccg tatttgaacg
     3661 cgaatttgag gaagtaaagc cgcgtggtac tgttccatac tcttcataaa aacgatttaa
     3721 gtaggtctta aattctgctt tcatcgttcc ttttagctga ggactcacta actgtacctg
     3781 atagaccact ttgtctaagt gtgtttgata ggacgctgct tcattgatct tcaaatcttt
     3841 gactgatcca tcgtcatata aactggatgc ttcatcatac gtccgtgtgg caaatatctc
     3901 taaaggattg atcattccgt cagatccgtc taaacgcacc ataataccgt tatattcctt
     3961 taagaaagga ataaagtctt ttcccttttc aaagccccaa ataaagtctc ctgcagctaa
     4021 atgggcttcc gcgatcattt taagaacggt cgatttacca gctcccatca ttcctagaag
     4081 caaggtatta gtagacaacc gtacactatt tttatacgtc gcattccaga caaatgggcc
     4141 gcctgtcatg gtttccccat attgtgtccc tcctggatct tttagcgttt ggtggtggaa
     4201 cgggaaacct ccgccaatgt ttaacgatgg tagtgattgc cctttgatat ttccgaacca
     4261 ttcagcttgt tcatcgaaca tggtaaacat cgattgccaa tctcgtttgg ttttatgcag
     4321 ataagtcacc gcacgataat ccatcccttt tagattttcg atcagttcct gcgctcgttc
     4381 atctacttct tgaatcgtgt ttccgtaaac atgtactctc actttgactt gcttagaaat
     4441 ttccccatta gaagtcaatt tacgagcata ttccgctagt tccaggtatt cttctgcgct
     4501 tttatttcgg tcagtcgctt ttcgttcatt atctgatcga tcatttaatt catctaaggc
     4561 acgattgatt tgatcaatca ctttttgctt ttcatctgtg ctaacatcga gtgtggccac
     4621 tgtatgagga ttgttcatga gattggccaa ccaaaaaagc gtaggatttt ttgtgtagtg
     4681 ataaactgtc acacatcgtg aataaccatt accatacgtc acatattctt catcaaaatt
     4741 taacccacct tgtggcatca cttgttttaa aaaatctaaa tcataccctt tggattttaa
     4801 aatttctatt tcccgatttc ctagttgctt taatggatca agtacttttt gatcattttg
     4861 tggtgaaaaa gctgttttaa ttgcttctaa catgtacagt cacctcttct tgtttgattc
     4921 tttacttggt taaaagattg cccttttccc aacttaccca ggcttaaagt gtgaggaaat
     4981 tttctagtgg tctcttcctt cggaacatct ttttctaacc atatcaacgg atatttcatt
     5041 ctctcttctc cattcctact catcattcat attgttcatt cgatacagaa tcttgatcaa
     5101 ttcttttttg tagagtggtt ttaaccctaa aatgccacta gatcgcatta actgacggtt
     5161 tctggtttgt aaatccttga ttgtctttcc ttcaatgacg aaaaagaagc ttaagtcagg
     5221 acgttcttgt tctgctcgtt tcaattcctg gatttcttcg actaatttct tttcatattt
     5281 ttttaatcgt tgtagatttc ttcctaagga ctgttccata cgatttttat ctaataattg
     5341 ccgcttttgc acgatttgtt ccgttagatc cgtctctgtg gtcaaactaa ctaacttaat
     5401 tgttggcaca tacgtacgca tgacggtggt caactgattc attcgttcta gtttctcttg
     5461 ttcatttaat ccttccacgt ctgtagaaac tacacgataa taacgttgaa aatgatcttc
     5521 tctagtaatt aagactccct cttctgtaca ggatttatac ggaaataagt ccaagataga
     5581 gcgctcttct ggttttccaa actgcaaaat ggatttcctt tcaactgaat tttgtccttt
     5641 gactcgcatt tattcatcct ccttatacgt accatgataa tttcggttag gatctaacgc
     5701 atgataatgg ttcttgtccg caaaaaaaat agactcgata atcgcaaaaa tttgtcgttg
     5761 acctgggtta ttatagggtc gccaaacaat cactaacgct atcgcaatcc ataagccata
     5821 attcaagagt gcaaaaaaga gtggtaagtg gacaagatta ttgaccattt tcgcccaaaa
     5881 aaagcagcca ccaagcgtta atagatctaa aaagaaaaaa gcgaaaaact tccattctcc
     5941 actaaatctt ggagggtttt tcatgtttat atatttatcc ataattgatt tctccttttc
     6001 atataaataa aaggcctaac ttgtaggcct tttatggttt gatatcctgt ttgatatccc
     6061 tcaatagttg ttctttcaat gcattgttcc tgtttttagt ttctcttgtc acatttattt
     6121 ttgttggtgc agtaaatgcc acatttgctg gcttgtaacc tgtatgaacg ttttgaatag
     6181 gttttggtgc agcttctctt gtcacaacat tttgaacgga cgcattccct gtctcaccac
     6241 tttcagataa attgtaagaa acagttgtca gatttccatt ttcaggcaaa cgacttggtt
     6301 tcatttgaac agtacctgca gttgattggt gaacaaagct ttgatcattc tccttgaatt
     6361 gattcaaacg actatctttc attggtaaat tttgtgcctt aggtaaacca attttcgttg
     6421 atttttcagt tccaacttcg attccattat catttggcag aacgatggaa tcctcatccg
     6481 tttgtacttc ttgatttcgt tttggtaaat ctgaaatgcg tttttcattt tcggagttaa
     6541 cagatctata ccgatattgt tctggatcag tttttggtaa ctgtccctca ttttctgctt
     6601 tgtcttcttg atttactttt gataaatctg aaattcgctt ctcattttca aggtcactgt
     6661 taggtttata gcgatattgt tctggatcag tttttggtaa ctgtccattt tttccttctt
     6721 ctgaatttct ttcttctatg gaatttttct cttcaatcga attttcatta gagtgcaacg
     6781 cttcttggtg atctttgact gaaggaatca ctataccttc ttgattttct ttttcggttg
     6841 ttgtattagc ttttactgct gcattaactt gtccttcacc atacgcatct tttagtcctt
     6901 ctttgacttc tgaagctttt tctttttgtt tttctagtgc ttcggatgga ttcttcacag
     6961 cttcagccaa ttctttagct ctcgtgccgt ctaatttctc ttctaaggtg tctttggctt
     7021 tttctgtaag atcttttcag ctgttccttt taacccacgt tcattgacgt aaccaagacc
     7081 ttctgcggct ttagaaacac catttttcgc tttcccgaac atttcggaaa ctgttttctt
     7141 actattaggt acagttgtag ctttatcgtc tgattgttct tccccgttat caggggtaac
     7201 atcttttcga tggccaaatc catttttacg aatatcattt aaatggctta aaccactctt
     7261 accagcactt gcaaggtttt tagctccgct acccacattt ttcgcaatat ttcctaaggc
     7321 aaacatgcta agaagagaat tactgccgtt tttcagactc gtatcaacac cgaaaatctt
     7381 cgtaaccgat tgagagcctt tgaatagcgc aatagttagc acaattaaag caacaacata
     7441 caagaatcca ttgatctttc ctgccgcatg caaatcaccg atacctgaaa gcattaatgt
     7501 atagattcgt aattctaaaa tcgtaaacgc caataacaag aaggattgag aaatatcttc
     7561 tatcgcactt ttcatccgtt ggcctgtctc aaagtccgtt gacgcggcaa tcactaaata
     7621 gaatttttta aataccagct caataatgca agtaacgatc gtaaatagga taaataaata
     7681 ggccaccgcc aaagaagatt caccagctaa gataatacct ggtttactac tgtatcgacg
     7741 ataccctgaa gtatagacat attttgctaa gccttcgtca ctaatttgaa caggaatttc
     7801 tttcccatta tcatctagtt ccaatcggta ttgtaacgct cttaaccgat ccttttgtaa
     7861 atctgataaa ccactcttat ctttatccgc catttcttta gcttgtttag gagtaataat
     7921 cccattgata ttcgctgttt taaaattttt cgctgttaaa gcatttcttt tgtctgttgg
     7981 cagggattct actttattcg ggtcagttgc tagtaatgta ctgacatcga tcacattatt
     8041 ttgaataatt tgaaaagcag gtgaacccgt tttatctgct ttatttgctg cagtgacgtc
     8101 tccgtaaaag cttttactga tatttaaccc ttcatcgatt aaagaactcc caccaaaaat
     8161 caagatcatg gccataaata aattaaccaa gatatttttg acttttggtg ggtgtttact
     8221 cataaaaaat ttcaagccta aataaattaa tgtgacgaca caaaaaagag ccaatacctt
     8281 actaatcaaa ctttgaaata gtgcatttac cccagcggcg ttcaataagt cttttaaatc
     8341 aaagctttga taaatggttt tttctagtaa agtattgaga ttatataatc ctttaatcaa
     8401 tccccacaga aaagtgttcc aaatccaact aaatacatct tgatactgca gataattacc
     8461 ataatcttgt aagactttcg ttacggtttt ggggtttgca tccccttctc cgaataagcc
     8521 tgaccaccag tcggctatgc cagaaaaaat tcctccaatg atcattcttt caccaccttt
     8581 tcagattcca ataacaaccg tttcccaatc atttcagaaa tttctttatt tttgatcaac
     8641 ttttcaacat attcttgaag tcgatctgct gcttgttctt cgtaaaattg aattaacaat
     8701 tttttagccg tctcattctc aagagattga acgattttat aaaacatatt ttcttcgttt
     8761 ttttcttcta attgatcatg cagtccatga tagagatttc tagcatattc atctggtagg
     8821 aaataatcat aatcaatttg taaagcctta agatccaagt ccgcatgtgt acaatgtatg
     8881 tctatatcat tgatatccca cgatggattg aaatcttcaa tatattgata agcataagga
     8941 agattcgttt ccccatgatt aaaaatagga tacggccgta ttttatgacc tttcaaatct
     9001 tgtcgtttta aaggagcgag tacaatcgtt tcaccttcta aaaaagtaga aattctttca
     9061 ggagttaaga tacgatctga atcaacattg cgattgatat gagtattcat acctaatgaa
     9121 ttgatggtac tgttctcttg ttcaaccgtc ttattcccca cttttttaga aaattcttcg
     9181 atggtattat catctgtcga cataatataa atatgtgtct gacaattttc tttgataatc
     9241 gctgcgtttt gttctccata cttcgcataa agttgctgat aagattgaat aaacagatcc
     9301 cacaacatgt ttcggccagc tgtgaccgtc atgattcctt ccatattatc cattggtggc
     9361 atattcccaa attcatctaa aataaaatga atcctgcggt gacatt
//
LOCUS       DACESX010000101         9361 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.101, whole genome shotgun sequence.
ACCESSION   DACESX010000101 DACESX010000000
VERSION     DACESX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.101"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..282)
                     /locus_tag="IXF88_002070"
     CDS             complement(<1..282)
                     /locus_tag="IXF88_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1508791.1"
                     /translation="MSITKNIKCTDEKTSHSIKQILNIKDSHITFSKDAISKERVHGR
                     MANVFTGLLTGEAHRCECCGFDSVIRHAYHDSWIQLLPYQEVPTYLHLYK"
     gene            complement(430..988)
                     /locus_tag="IXF88_002071"
                     /pseudo
     CDS             complement(430..988)
                     /locus_tag="IXF88_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302360.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1157..3268
                     /locus_tag="IXF88_002072"
     CDS             1157..3268
                     /locus_tag="IXF88_002072"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012579.1,HMM:NF013070.1,HMM:NF019826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1508792.1"
                     /translation="MNTKDLKLLETIVRSHVTNSQQLKSLVHFSTRQITYSIKKINDE
                     LSLHGLSLIKRSNSGEWLYEANDLQKLLIYMNQAMVFKEGAIPEYINDDLAYSEIRRY
                     SELLFILANPKKVTTQEIMAFIGISKNTARNDFSKIKKELPPDYTLIFDPQNGYMIES
                     SMAKLHTLLLEVFNYLFERHATVNRFESLFSEEREFVGFFIHTYEQTFKLKFSDNSLT
                     KLYYLIGLSLSSFKRRTKGQIDFSYDLQELDEFQMIRSCIDQHYQHAEIAIDYEWLTL
                     LFLSFNTISNEFEILDSRIYQVIMEMITLFQSKSAIRISNKEELTNRLMNHLRPAIYR
                     IRYELPFEIDDLAEIVKENHEVSAVLNFVKESIVPLEAFLRKTIPETELYLISVYFCS
                     ELLNSSIIETPVRKKAIVVCSNGLIMANVMAKTLKEIFPDIQFVASSSVREMDKFVED
                     VDLIFSNQPLKSEIPLYIIQPIMNEEEKNRLRNRVLIDFSTNNISKETRNLTEIIKRY
                     SAVQNEESLQTDIERFLINYANSLIYLDRQSKQAQKISFVELLVSEGIILVSTKQLTW
                     QNAMELSCQPLLKEQVINQNYQTILMSQMADEEAYYFLNNRIAIPHSSPENGVIKGGC
                     SLLISVDPIIFPGNRPIHFISPFAIGKKENYLLAINQLLQLSKDEEYQQEILLCTTED
                     EVRKKIKNFLRRKQYVNNEFS"
     gene            3246..3896
                     /locus_tag="IXF88_002073"
     CDS             3246..3896
                     /locus_tag="IXF88_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1508793.1"
                     /translation="MSITNFPELTIILRGYSFEEAMFVMEELAAMNTSVGIEVTTNND
                     DFARIITEGNKKFGQQLLIGAGTVLTFSQADQAIAGGAKFLLGPKKFSTPIFSLAKER
                     GVVTVPSAFTPSEIQVMFDEGADIVKVFPAVTVGPTYFKQLAGPFGIRPLMAVGGVTI
                     DNIAAFKKAGVVYFGVGSSMFQKADIQNRNGQAIRKSIQSYLDKLAEAGSEEDESH"
     gene            3883..4335
                     /locus_tag="IXF88_002074"
     CDS             3883..4335
                     /locus_tag="IXF88_002074"
                     /inference="COORDINATES: protein motif:HMM:NF012579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1508794.1"
                     /translation="MSLIKLEEKNILLNQSFSNQEEYFQKITKKLMTENMVKEGYLES
                     ITKRETTYPTGLYIGERSIAIPHTDYQLSNVTAILVSVLKDSLHFQRMDDPEEMVSVN
                     VIIHLLFDSPDKQPQLLKELMSKVTNQDFLQAIMEAKSKTEVLTAFNQ"
     gene            4352..4657
                     /locus_tag="IXF88_002075"
     CDS             4352..4657
                     /locus_tag="IXF88_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HAQ1508795.1"
                     /translation="MSKKTIIVACASSMITSTMAANKIRKIAKTNGLPEPRILQLKFS
                     EVAGALESNQVDFIVPTGKLQENVTKGIKVIPGTPFVTGVNEEKSESLVLEALKELD"
     gene            4669..5946
                     /locus_tag="IXF88_002076"
     CDS             4669..5946
                     /locus_tag="IXF88_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010492829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IIABC"
                     /protein_id="HAQ1508796.1"
                     /translation="MNIVIDALNWISSLGPMIMMPIIILILGLFFRIKLNTLLKSALT
                     VGIGFAGVNIIINWFISQVGPSVQLMVKNWGIQTSIMDVGWPARAAATWSFPLAAVMV
                     FIILGINVLMLIFKKTNTVMVDFWSYNHYIFVAALVYYATDKNIILAVIFGAIDAIIS
                     FKLADWTTPLVEDYFELEGVNFPTANSVGWAPLAWLLNKLWDRIPGLNKVDARPEKIQ
                     ERFGFVGEPMFMGFVIGLVIGLLAKEPVDKVLIVGMSTSATMVLTPKMMQILMEGLLP
                     FANSVKKFLNVKFPGNNFTMGVDAALTVANSSAIATGIIMVPITLVLAAILPGNKLLP
                     ISDIAYQAMWLSAWPVAFSKGNIIRGILSTTIITACVLLIATSLAGIHTQLALAGGFQ
                     LPAGMTQISTEDAGTHIIGFILMKIAEFISNIF"
     gene            5965..7113
                     /gene="dgoD"
                     /locus_tag="IXF88_002077"
     CDS             5965..7113
                     /gene="dgoD"
                     /locus_tag="IXF88_002077"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ1508797.1"
                     /translation="MKISKITVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRFLVGQNPFAIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKALNVPV
                     YELLGGAARDKIKVYSWIGGDRPSEVVEQAQDRVDRGFTAVKMNATEELHYIDSYEKV
                     AEVVERVASIRERFGDTLSIGVDFHGRVHKPMAKVLAKALEPYHPMFLEEVVLPENEE
                     HFSEVANAVAVPLATGERLYTRWQFKSILQQGAIDIIQPDVALCGGILETRKIAAMAE
                     AYDIAVAPHAPYGPIALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEIFQ
                     YKDGFVDLPTKPGLGLEMDEDRVKEIAQEGLVWTNPKWKNYDGTIAEW"
     gene            7125..8027
                     /locus_tag="IXF88_002078"
     CDS             7125..8027
                     /locus_tag="IXF88_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HAQ1508798.1"
                     /translation="MINTVTGPIEAESLGTTFIHEHLYVIPNELRKYEDYTLDDIYHS
                     IAEVMAFKQAGGKTIVDLTPINYGRNPLALKMISEATGVNVAFVTGFHKEEFQPKWLT
                     TLNDDEIFELLYSEITHGVTSQNLLPGAMKVGTSKNEVTLAEQRILAIAGQVQKKTGI
                     PIITHCDGGTMGLEQINCLTKNGAKASNICLSHVDLTKDFHYLAQICETGASICMDHI
                     GRELANHDADRVQLIKRLVDAGYENQICLSGDMGRKKYLPTYGGTPGLTYILTSLKEE
                     LLKVMSEPTFNKMIEMNPINILSK"
     gene            complement(8696..9082)
                     /locus_tag="IXF88_002079"
                     /pseudo
     CDS             complement(8696..9082)
                     /locus_tag="IXF88_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9142..>9361)
                     /locus_tag="IXF88_002080"
     CDS             complement(9142..>9361)
                     /locus_tag="IXF88_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1508799.1"
                     /translation="EHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRG
                     IYKKNRRNGTLFGFSVSTEIKVLMGITA"
BASE COUNT         3016 a         1453 c         1909 g         2983 t
ORIGIN      
        1 tttgtacagg tgtagataag ttggtacttc ttgatacgga agcaactgaa tccaactatc
       61 atgataagca tgacggatga cagaatcaaa cccacaacat tcgcaacgat gcgcttctcc
      121 agttaataag ccggtaaata cattggccat acggccatga acgcgctctt tactaatggc
      181 atcttttgaa aaggtaatat gggaatcttt gatgttcaaa atttgtttga tggaatgact
      241 tgtcttttca tccgtacatt tgatattctt agttatggac atttggttat cctcctatga
      301 ttgtttttgc cgatttaatt ataggacaac cagatgtctt tttgttataa aaaaatattt
      361 ggcgtcacta ctcagtttga gtaacaaaac caaatattat agaaccggct ttttcagttt
      421 tttcccttca caaacaacat cgatgatttt ctcggcatgg gtacgggtat cattcattaa
      481 ttctgtatcg gttggatatt tgatgttttg tggagcacaa gtggcatcca aaatcaatgt
      541 tccggaattg aaatcgtcat aagaatcgtt gtccttatcg tcttttttct tgtcgaagac
      601 gagtattttt tcattgattt ctatcaacgt tgtttcgtcc aagcgtttcc ggaaatagac
      661 cattgtagag catcgaaagg cttggcatct tgatagcccg gcaaaccgat gaagaactat
      721 aaatacgggt tttcttgaat ttgtaagacc gtttcttcgt cgctatagct atattccttt
      781 tgaatcagta gagctccgaa ggtcatgcga aagggttttg cgacgtttcc ttttttttac
      841 gaaaattttt agcgtagtct ttttcaagtt cagcccatgg aatgagctct gctttttgaa
      901 tccaacggtt agacggattc attttgagac ctatcggttg attgaaatct tcaaaagcca
      961 attgaacggt tgtttgtaat ttatacatga aaagcctcca aaattgagtg gtactccaca
     1021 ccattctgat ggtgcataag ataaaaaatt aaagaagtgc ctgtcatttg tctgatttaa
     1081 gccaaggttt gacaaatttt ggatattcca attaatactg taagcggtta tatttaactc
     1141 agggaggtag atgaatgtga atacaaaaga tttaaaactg ttagaaacca ttgtacgatc
     1201 acatgtgaca aactctcaac aattgaaatc attagtacat ttttcgacta ggcagataac
     1261 ttactctata aaaaaaatta atgatgaatt atcccttcat ggattgagtc tgataaagcg
     1321 aagtaactca ggggagtggt tatatgaagc caatgatctt caaaaacttt tgatatacat
     1381 gaatcaagca atggttttta aagaaggtgc gattcctgaa tatataaacg atgatttggc
     1441 ttatagtgag ataagaagat attcggaact gttatttatt ctagccaacc ccaaaaaagt
     1501 gacaacccaa gaaataatgg cgtttattgg tatcagtaaa aatacggcta gaaacgactt
     1561 ttctaaaata aaaaaagagt tgccaccaga ctacactttg atatttgatc cccaaaatgg
     1621 ctatatgatt gaatcaagca tggcaaagct tcatacattg ctcttagaag ttttcaatta
     1681 tttatttgaa aggcatgcaa cagttaaccg ttttgagtca ctcttttctg aagaaaggga
     1741 atttgttggt ttttttattc acacctacga acaaactttt aaattgaaat tttctgataa
     1801 ttcactaacc aaactttatt atttgatagg tttatcactt tctagtttta aaagaaggac
     1861 gaaaggacag attgatttta gttatgattt acaggaatta gatgagtttc aaatgattcg
     1921 aagctgcatt gatcaacatt atcaacatgc tgagatagct atagattatg aatggttgac
     1981 gcttctgttt ttatcattca atacaatttc aaatgagttt gaaattttag attcaagaat
     2041 ctatcaagta attatggaga tgattacctt atttcaaagt aaatcagcta ttcggattag
     2101 taataaagaa gagttgacca atcgattgat gaatcattta cgacctgcaa tctatcgaat
     2161 tcgctacgaa ttaccatttg aaatagatga tttagctgaa attgttaagg aaaaccatga
     2221 agttagtgcg gtacttaatt ttgttaaaga atccattgta cctttggaag catttttgcg
     2281 taagacgatt cctgaaactg aattatattt gatttctgtt tatttttgtt cggagctatt
     2341 aaattcttct attatcgaaa cacctgttag aaaaaaggca atcgtcgtat gtagcaatgg
     2401 attgattatg gccaatgtaa tggcgaaaac attgaaagaa atttttccag atattcagtt
     2461 tgttgctagt tcttcggtca gagagatgga taagtttgtc gaagatgttg atttgatttt
     2521 tagcaatcaa ccattgaaaa gtgaaattcc attatatatt atacaaccga ttatgaacga
     2581 agaagaaaaa aaccgcttgc ggaaccgtgt actcattgat ttttctacga ataatatcag
     2641 caaagagact aggaacctta ctgaaattat taagcgttac tctgctgttc aaaatgaaga
     2701 aagtctacaa actgatattg agagattttt aattaattat gcaaattcac taatatatct
     2761 agatcgtcaa agtaaacagg cacaaaagat atcttttgtg gaattgcttg tttctgaagg
     2821 gattattctt gtatctacga aacaacttac ttggcagaac gcgatggaat tgtcttgtca
     2881 gcctttatta aaggagcagg taattaatca gaactatcag actattttaa tgagtcaaat
     2941 ggcggatgaa gaggcttatt attttcttaa taatcgtatt gctattccac attcatcgcc
     3001 agaaaatggg gtaattaagg gcggttgctc gttacttata tcagttgatc caattatttt
     3061 tcctggaaat aggcctatcc attttatttc cccttttgca attggaaaga aggaaaatta
     3121 tttacttgcg attaaccaat tactacaact ttctaaggac gaggaatatc agcaagaaat
     3181 acttctttgc acgacggaag atgaagtgag aaaaaaaata aaaaattttt taaggaggaa
     3241 gcaatatgtc aataacgaat tttcctgaat taaccatcat tttaagagga tatagttttg
     3301 aagaggcgat gtttgtcatg gaagaattgg ctgcgatgaa cacttcggta ggaattgaag
     3361 taacaaccaa taatgatgac tttgcacgta tcatcacaga aggcaacaaa aaatttgggc
     3421 aacaattatt gattggtgca gggacagttt taacattttc acaagcagat caagcaattg
     3481 ctggtggtgc gaaattttta ttaggaccaa aaaaattttc aacaccaatt ttttcgttgg
     3541 ctaaagaacg tggggtggta actgtgccat cagcttttac gccatcggaa attcaagtta
     3601 tgtttgatga aggtgccgat attgtaaagg tttttccagc agtcactgta gggccaacct
     3661 actttaaaca actggccggt ccttttggta tacggccttt aatggcagtt ggaggagtga
     3721 cgattgataa tattgcggct tttaaaaaag caggagttgt gtactttggg gtaggatcaa
     3781 gcatgtttca aaaagctgat atacaaaacc gcaatgggca agctattcga aaatcaattc
     3841 aatcatattt agataaacta gctgaagcag gaagtgaaga agatgagtct cattaaatta
     3901 gaagaaaaaa atattttgtt aaatcaaagt ttttcaaacc aagaagaata ctttcaaaag
     3961 attacaaaga aactgatgac ggaaaacatg gtaaaggagg gatatctcga gtcaatcact
     4021 aagagagaaa caacttatcc aacggggcta tatattgggg aacgctcaat cgcaattcca
     4081 catacagatt atcagttatc aaatgttacc gctattttag tatccgttct taaagatagt
     4141 ttgcattttc agcgaatgga tgatcccgaa gaaatggttt cagtgaatgt tattattcac
     4201 cttttattcg atagtcccga taagcaaccg caattattga aggagttgat gagtaaagtt
     4261 accaatcagg atttcttaca agcaattatg gaagcaaaga gtaaaacaga agttttaact
     4321 gcatttaatc aataaattgg agggaaatac aatgagtaaa aaaacaatta tcgtcgcatg
     4381 tgcatcatca atgattacat ctacaatggc agctaataaa attcggaaaa tagcaaaaac
     4441 aaatggatta ccagaacctc gaattttgca actaaaattt tcagaagttg caggtgcttt
     4501 agaatcaaat caagttgact ttatcgttcc tacagggaaa ttacaagaaa atgtaaccaa
     4561 aggaattaaa gttatacctg gaacaccatt tgttactgga gtgaatgaag aaaaatctga
     4621 atctttggtg ttagaagcat tgaaagagct tgactaggag ggtgaaaaat gaatattgtc
     4681 attgatgcgt taaactggat ctcaagtttg ggtccgatga ttatgatgcc tatcattatc
     4741 ttgattttag gattattttt tagaattaag ctaaatacat tactaaaatc agcgttgact
     4801 gtggggattg gttttgcggg tgtaaatatt attattaatt ggtttatttc acaggtaggt
     4861 ccatctgttc agttaatggt caaaaattgg ggtattcaaa caagtattat ggatgtcggc
     4921 tggccagctc gtgcagcagc cacttggtct tttcctctag cagcagttat ggtatttatt
     4981 attttgggta ttaatgtgtt gatgctgatt tttaaaaaga ccaatacagt aatggtcgat
     5041 ttctggagtt ataatcacta tatttttgta gctgctttgg tttattatgc aacggataaa
     5101 aatattatat tggcggtaat cttcggggcg attgatgcaa ttattagttt taaactcgct
     5161 gattggacaa cgccgttagt agaggattac tttgaactag aaggggttaa tttcccgact
     5221 gcgaactcag ttggttgggc acccttggct tggctactta acaaattgtg ggaccgcatt
     5281 cctggattaa ataaggtaga tgctcgtcct gaaaaaattc aagagcggtt tggttttgtt
     5341 ggtgagccta tgtttatggg atttgtcatc ggcttggtta ttgggttatt agcaaaagaa
     5401 cctgttgata aggtattgat agtgggaatg agtacttctg caacaatggt tctaacacct
     5461 aaaatgatgc aaattttaat ggaaggatta ttaccctttg ctaattctgt taagaaattc
     5521 ttgaatgtaa aatttccagg aaacaatttt acaatgggtg tggatgctgc gttgacagtt
     5581 gctaattcaa gtgccattgc tacgggtatt atcatggttc cgattacctt agtgttggct
     5641 gctattttac caggtaacaa actcttacct atttcagata ttgcctacca agcaatgtgg
     5701 ctatctgcat ggccggtcgc cttttcaaaa ggaaatatta ttcgcggaat actttccacc
     5761 acgattataa cagcttgtgt gcttttaatt gctactagtt tagccggcat acatacacaa
     5821 ttagctttgg ctgggggatt ccaattacca gcaggaatga cacaaatatc taccgaagat
     5881 gcaggtacac acattattgg tttcatttta atgaagattg ctgagtttat ttcaaatatt
     5941 ttctaatatt caggagggca tacagtgaaa atttcaaaaa ttacagtcta taaagtaaaa
     6001 ccaagatgga tttttgtcaa aatatctaca gatgaaggta ttgaaggctg gggtgaaatg
     6061 atttctggaa ctaagactga aacagttgtg gcaggagctt atgagatagg aaatcgattc
     6121 ttagttggtc aaaacccatt tgcaattgaa cgtctgtttc aggaaatgca tcgttcattt
     6181 ttccgaggtg gtcctattaa tggaacgatt gtctctggac ttgaaatggc tttatgggat
     6241 attaaaggga aagccttaaa tgttccagtt tatgaattac taggtggagc agcccgtgat
     6301 aaaatcaaag tatattcttg gattggtgga gatcgtcctt ctgaagtagt agagcaggca
     6361 caagatcgag tagatcgtgg gtttaccgcc gtgaaaatga acgcgacaga agaattacac
     6421 tacattgatt cttatgaaaa agtagctgaa gtagtggaac gtgttgcttc aattagagaa
     6481 cgttttggag atacattgag cattggtgtc gattttcatg gtcgtgttca caaaccaatg
     6541 gctaaggttt tagcaaaagc actggaaccc tatcatccaa tgttcttaga agaagtggtt
     6601 ttgccggaga atgaagaaca tttcagtgaa gtagccaatg cagttgctgt accactagca
     6661 acgggtgaac gtttgtatac aagatggcaa tttaaatcaa ttcttcaaca gggagcgatt
     6721 gatatcattc aaccagatgt agctttatgt ggagggattt tagaaacaag aaaaattgct
     6781 gcaatggctg aagcttatga tatagcagtt gccccccatg caccatatgg accgattgcg
     6841 ttagcagcta cattacaagt tgattcttgt acacctaatg tgtttattca agaacaaagc
     6901 ttaggtattc attacaacaa agggtttgat ttgttagatt ttgtaaagaa taaagagata
     6961 ttccaataca aagatggctt cgtagatctt ccgacaaaac caggtttagg gttagaaatg
     7021 gacgaagatc gagtgaaaga aattgcacaa gaaggtttag tttggaccaa tcctaagtgg
     7081 aaaaattatg atggtacaat cgctgaatgg taggagatga gtgcatgatc aatacggtta
     7141 caggcccaat cgaagctgag tctttgggta caacatttat tcatgaacat ttatatgtca
     7201 ttcctaatga gttaagaaag tatgaagatt ataccttgga tgatatttat catagtattg
     7261 cggaagttat ggcttttaaa caagctggtg gaaaaactat cgttgatttg acaccaatta
     7321 attatggacg aaatccgtta gctttgaaaa tgatatctga agctaccgga gtgaatgtag
     7381 catttgttac gggattccat aaggaagagt ttcagccaaa atggttgaca acgctcaatg
     7441 atgatgagat ctttgagttg ctttattccg aaataactca cggagtcact tcacaaaacc
     7501 ttttgccagg agcaatgaaa gtaggtacga gtaaaaatga ggttacattg gctgaacaac
     7561 gaatactagc catagccggt caagtacaaa aaaaaacagg gattcccatt attacgcatt
     7621 gtgatggggg gacgatggga ctggagcaaa ttaattgttt aacaaaaaac ggtgcgaaag
     7681 caagtaatat ttgtttgagt catgtagatt taactaaaga ttttcactat ttagctcaaa
     7741 tctgtgaaac gggagcaagt atttgtatgg atcatattgg ccgagagttg gcgaatcatg
     7801 atgcagaccg cgtccaacta attaaacgac tggttgatgc gggttatgaa aatcagattt
     7861 gtctttcagg tgatatggga cgaaaaaaat atttgccaac ttatggagga acgcctgggc
     7921 ttacctatat tttaacaagt ttgaaagaag aactattaaa agtaatgagt gaaccaactt
     7981 ttaataaaat gattgagatg aatccaatta atatactgag taagtgaagt caattgaatc
     8041 attcagaaga actaatgaaa ttagttgctt tcgcagggtt aattcttctc tctgggtttg
     8101 gaaaagaggg cttagcttcg gattccctag ttaaactagg ggttttatca ggttattgta
     8161 ctgattataa gtacctgaat tattcatgaa atactttttc cgtcgtttta taggcagttg
     8221 ggtcaataaa aagatcagaa aaacgaacga gagactctca taacgtgcca ctaaataaaa
     8281 atcgtacgtt attttaaaaa atgggttgat tctaggcagt actttgtcgg tctattttta
     8341 gaaaaagagg ttctttgtca aacatacatg aattcgcaag ctcattcacc ctgaaggatg
     8401 aaaggcagaa actgtagtat actaatggtg gaaaggaaaa aaagccgcca gcaatttgga
     8461 aaagaacgtt atgtcgattg tcaaactggc ttgctttatt tctcgagaac cctgaaatat
     8521 agccaatgag catgtgtata aaaaattgat tttatatgaa ataaagcact cctttcctaa
     8581 aattattgga aaggagtgcg ttttttatgg aaattattta ggttctttgt caattgctat
     8641 tgttttgctc aaatcgtatg ttaggaaatg cttcggcatt tccttttttg cgttattaaa
     8701 tgatttcaca gatgttctgt tgaatcagga cacgcatccg gagatgacga aaagtccgaa
     8761 atccaaaagc aattcttttg attactttga tgaggttatt ctttccctcc aattttccgt
     8821 ttgaataggg atattttaag gagtttgtga ttgcttctgt gtgctttttc agataacaaa
     8881 ttgccttttt aaacccatca ttcaaggaat gtggcaatga ttcaatgaga ttgaaaaatt
     8941 cagtaaaatc ttttgcatca aaagcagcta ataactcttg atagacttca tagctttgtc
     9001 gtagttggtt atctatagag agtaggtaat caagcacctc cgactccgtc atgtatttcc
     9061 cttggaagag gggaaattgc ttaaaagggt tctgttgcga agtttgaaaa tcaaacgcgc
     9121 cctctctgaa ctccaaatat cttaggctgt tattcccatt aataccttga tttcagtaga
     9181 caccgaaaag ccgaagagcg ttccatttct tcggttcttt ttatatattc ctcgaatggt
     9241 ctccatgccc ttaatcgtgg aagaggctgt acggagactt tgataaaatt tatttcgtcg
     9301 tttaataggt cgatggtctt gttctattaa attgttaaga tacttcacag ttcggtgctc
     9361 t
//
LOCUS       DACESX010000102         9196 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.102, whole genome shotgun sequence.
ACCESSION   DACESX010000102 DACESX010000000
VERSION     DACESX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.102"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(35..328)
                     /locus_tag="IXF88_002081"
     CDS             complement(35..328)
                     /locus_tag="IXF88_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1508800.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(356..1021)
                     /locus_tag="IXF88_002082"
     CDS             complement(356..1021)
                     /locus_tag="IXF88_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1508801.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1161..1793)
                     /locus_tag="IXF88_002083"
     CDS             complement(1161..1793)
                     /locus_tag="IXF88_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1508802.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1800..2867)
                     /locus_tag="IXF88_002084"
     CDS             complement(1800..2867)
                     /locus_tag="IXF88_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1508803.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(2864..3595)
                     /locus_tag="IXF88_002085"
     CDS             complement(2864..3595)
                     /locus_tag="IXF88_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1508804.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDIEVIRI"
     gene            complement(3763..4242)
                     /gene="greA"
                     /locus_tag="IXF88_002086"
     CDS             complement(3763..4242)
                     /gene="greA"
                     /locus_tag="IXF88_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1508805.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4378..5553)
                     /gene="mltG"
                     /locus_tag="IXF88_002087"
     CDS             complement(4378..5553)
                     /gene="mltG"
                     /locus_tag="IXF88_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1508806.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5718..6878
                     /locus_tag="IXF88_002088"
     CDS             5718..6878
                     /locus_tag="IXF88_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1508807.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6918..7865)
                     /locus_tag="IXF88_002089"
     CDS             complement(6918..7865)
                     /locus_tag="IXF88_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1508808.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7898..>9196)
                     /locus_tag="IXF88_002090"
     CDS             complement(7898..>9196)
                     /locus_tag="IXF88_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ1508809.1"
                     /translation="SIFFKLIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVIT
                     SFVHKEYGLGENLKTIMWAILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMS
                     MVLFFGKFGYEKYYNARHRIRIGFLESRLFGTFSDINNAAIASLVVIVLILFYLYNKK
                     VTGLNKIIFLINLPLQFIYIVLSGSRSTYVVSIIIVAISSFIVVLKRNGTKKDLKNYI
                     LAIVSSIGSVLILFALFNATKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQT
                     PDNTLVRKDVAENTDISNARITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIA
                     KVGIAIHNAYLNVLTSTGILGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVI
                     LSYALYAALNNELVYENTVGTFVFWLFLGRVNSKNNSSYF"
BASE COUNT         2832 a         1876 c         1388 g         3100 t
ORIGIN      
        1 gaaggtgaca aaaattgtgt caccctctct ttttttattg ttttttaaag tgataggttg
       61 gctcctccag gttgggatca taatcaacaa gtaaatcata tcctttaaag aaccattcat
      121 ctgcttcctc gataaaaaaa agtgtcccat ccatttccac tttctttaca ggatgttctg
      181 gctgttcaac tgacattcca atcgaaaaac catcatgaac ctctgttttg ccgtagactt
      241 ttccaaaaaa acgtattccg taagtttctg gcagatcaat ttctgagtca aaccactttt
      301 gtgcttttgg cgttaccgtt aatttcatcc caacaaccta ctttctcttt tctttctatt
      361 gattcatctt atcgtctaga atatccacat cgcctgtctc tccaataacc agtagattat
      421 cattttcacg aacatattca tctgctgcag gagaagcgat aaccgtttga tctactcttc
      481 gtatggcgac tactgttaag tcaaaacgtt gcctgaaatt taattcagct aatgttttgt
      541 tgaaaaattt aggattggag actcggattt ctgctaaaga atatttatct gacagctcta
      601 agtagtctag aatattcttt gatactaagt tatgtgcaat ccgtatcccc atgtctcgct
      661 cagggtgtac gacacgatct gctccgatct tctctagtac acgggcatgg tactcatttt
      721 gggcttttgc taaaatattc ggtacgccca tttcctttac catcagagta actaagatgc
      781 tggcttggat atcttctcca atagcgacaa ttacatgatc gaaatttctt atacctaatg
      841 aacgaagtgt catctcatcc tgtgcatttg ccacaacagc atgggtggct atattcatat
      901 attcattgac tcgatcttca ttactgtcaa tagccagtac ttcttgtcca gcttcgatca
      961 gtgtacggca gatgcttccg ccaaatcgtc caaggcctac aattgcaaaa ttttgtttca
     1021 ccattttcct cctttagtat cttcttttct cagtataaca aagcctttcc aaaaacaaaa
     1081 gcaagcatca ttattgtaaa aaagccaaga aaaataatag ttattttctc agcttttgat
     1141 tatcctcttt atcatcgttt ttatttcacc aaaccatgtt taaacgcata gatagctgct
     1201 tgagttcggt cttctacctc taacttagct aatatattcg acacatgcgt tttgactgtt
     1261 ttcagtgtga taaacaattc atccgcgatt tcctgattac ttttcccttc agaaatcagc
     1321 atcaaaattt cattttctcg attcgttaat tcttcatgca atactggatc atttcgccga
     1381 ctcatttttt ccatcatttt tgttgtcact tcaggttcta gtactcgctc gccgcgttgc
     1441 gtagccctta tagcatcagc tatttcatgt gctgtagacg tcttcaacaa atatcctgct
     1501 gctcctgctt caattgcagg ataaactttt tcatcatcaa taaaacttgt gacgataatg
     1561 attttggctt ttggccaatc ttttaagatt gcttttgttg attcgatgcc atccatttct
     1621 tccatgacta gatccattaa aatgacatcc ggtcgaagtg ccatcgcctt ttcatagcct
     1681 tgacgtccat tttcagcttc tccaatcact tcaatgtctt cttggataga taaataagaa
     1741 gagacgccta agcgtaccat ttcatggtca tctactaata aaacttttat catctttatc
     1801 tatgcctcct tcatcaaagg gaccttggtc tccacgctcg taccttgccc tttaaaacta
     1861 atgattttta ctgttccacc aataccagct actcgttctt tgatattgtt caatccataa
     1921 cttcccgttt tggtttcatt catattaaat cctgtcccgt cgtcaatgat acgtaataaa
     1981 agattgttat ctattttatg caaatagact tctaattcat tagccttagc atgtcttaat
     2041 gtgtttgata acaattcttg aacgattcgg aacaagtgat cctcaatgga actagttaat
     2101 tttacatctt ctacctccca ttttagcgaa atctggattt tattctgcaa ttccttcaat
     2161 aactgctcaa tgccttgttt taaacttttt tcttctaaat tgactggacg cagatgaaga
     2221 agcagcgcac gcatctcaga ctgggaagcg ttgatgatct ctgccaccat tttcaactgc
     2281 ttacggaaca tttctggtgt ctcacttttt tctgcttgct ccgtcaatgc cgacatcatc
     2341 atcatcgctg caaataattg ctgtgaaact gaatcatgaa gctcacgagc taatcgatgc
     2401 ctttctaatt ttaaaatctc ttctttggtt tgtccatcta catattgagg acggctagtc
     2461 ataatctgta attcactgga tacttcgatc attttttctt ttattttagt aatttccata
     2521 tcaatatctt tgatgtacag atcatccgac gcgcttggga tcggctgatg caaaagtttg
     2581 gattcataat tgccagaaga aagtgcacga agcttttctt cgattttccc gtattttgcc
     2641 ttttgaataa gtgataacaa tagaaatacg atcaatccta cacctaatga aattgccatc
     2701 aaatggaaga taagcggaac gtataataaa cgcgcacgca gcaattctcc ccaccaatga
     2761 ctttggttac tggcataaaa ataagtgaaa agtgaaaata gaataataag gaaagccgca
     2821 atccccgaat aaacagctag cattgctttg gatattcttc ccatcatatt cgtatcacct
     2881 cgatatctcc aaccaaagta ttggtaatga tcttcaatct gcgaggattt tcatcataat
     2941 cactgctgca gattttgatc tgttcgtttt ttaaggaaaa ctgctcttct tcaaacaaga
     3001 catttcctac taatgtcgca tgctctaaac gaatagccac tcccaggggt actaagatcc
     3061 gtgttctgcc tatccctttt cttacaatca caatattatc atcttttggc aaaagtgtat
     3121 tgcccaaatc gatgatagta tcaccagaaa tgatggcaat gttgatatca tcccattcat
     3181 agacgtcgtt tccaatacgt tggttaccaa ataattgctg tcttttacgc tcattattat
     3241 gtgtttttaa ttgctctgtt tggaccatca tgatctgttt ttttcgccaa aaagcatttt
     3301 ttgtcaaatc tattcctgaa atctctacac ctttcagccc gataaataaa acggccaata
     3361 caaccatcat ccataaagca ggactgttga ctaagctaaa aaaaatgacc aagctcccta
     3421 agatcaattg aaaattttga aatttagtac gaggatattt gcgcatagct agatatatat
     3481 tgaaaatacc gaatattact aaaagcaaaa gtccagtatt gtttacgatt tgccaaacag
     3541 caaataacag cagcagtgct tcgactacca caaaaaaacg ccaagaacta ttcatcgctt
     3601 tttctccctc ctgacacatt ctcatttcat catacctatt tttttgactg ttggctatag
     3661 gactttagat gtaagatcgt tcagtgaaac ctgcaatact caaagagaaa agatctagag
     3721 tgcgtcatca gtaagatgaa ctctctagat ctttcagtca gactacgctt gttctacttt
     3781 gacgatttta actaacatat cgccacctgg tgtagcaatg gttacttcat catttttacg
     3841 tttgccaatc aatgcttgcg cgataggaga atcattggaa atctttccag aaaacgggtc
     3901 tgcttcagca cttccgacaa tcgtgtactc ttcttcatct ccatcaggca actcgatgaa
     3961 cgtaactgtt ttaccgatcg atacctcatc tgcatctacg ccgtcattat cgatgatttg
     4021 tgcaaaacgg atcatatttt caattgttgt gattcttcct tcaacaaatg cttgttcatc
     4081 tttagctgat tcgtattcgg agttttcgga aagatctccg aaaccgcgag cgattttgat
     4141 tcgttcaatg atttctccgc gtttaactgt ttttagttct tctaattctt tttctaattt
     4201 ttctttacct tctagagtca ttggataaac tttctctacc atgtgtaacg tctccttttt
     4261 tcgataaaat accgcaaaag gtaatctttt gaggaatata gtccaaaaaa gattaccacg
     4321 cttttcttaa aatgtaaatg tttttctaaa aatgatcaat gactagcccc tgtttttcta
     4381 agaattattt acatattttt gcactagttc catatgttct tcatacgttt tagcgaaata
     4441 aacatttcca gtgctcgtat ctgctacgaa ataataatag tcattttctg ctggttctaa
     4501 tactgccttg attgcctctt cgcttggatt gtcaaaagga ccaggcccat agccggtatt
     4561 ggtatacaaa ttgtatggtg aatctacagc tgtatcttca tacgtcacca attctttatg
     4621 ttctcctaat gcatataaaa cagagatatc tgactgaagt ggcatatttg ccttgattcg
     4681 gttaaagaag acttgtgcaa tatttttacg atcattttct tttacgcctt ctttttcaac
     4741 tagagaagcc aaagttaaaa cttcttgtac agtcaaattt ttctgtttga tagctgaata
     4801 atagttttgc atgaccgtat ttgttttgtt caccatctca atcacaagat ccttaagcga
     4861 agtactcgtg tagtaatcat aagtagctgg gaataagtag ccttccaaac ggtattttgt
     4921 gttctcagct ttagaggcac tttccaacaa ttctgggaat ttttgataca actcgttaaa
     4981 gaaagtctca tcatttacta aatctaaaaa ttctttcttg tctttaccag ttaccttcgc
     5041 tacccgctca gcaatctgat cgatgtcgta gccttctgga atggcaattt tcccatctgc
     5101 aacttttgtt ggctcagatg tgccgccttc ttgcaactgc ttgccaatct catcaagagt
     5161 catattaggc gctaactgat aataaccagc ttgaaagcca gttaagtttt taaattttgt
     5221 ataataattg aaaacaatcc cacttttaat aatattgtct ttctccaaaa tttcgccaat
     5281 ctgcttatta gaagagccgc ttgggatttc cacttggact aattggtcgt ccgatttatc
     5341 taaaggcttc aacccgctat ctacataacg atatacggta aacccgagaa atccgccaat
     5401 aatcaataat gtaagagcga tcactaaaat gatcttcctc acgatcttat cttctttttt
     5461 acgcagattt ctccgcttgt cgattatatc tgctgtagct gccttactgg cttctttttt
     5521 gtctttgctt gggtgttgtt tgtttcctga caaaacaact cctccttact atcatcttcg
     5581 taactctgtt tattatacat gaaatttcta ctgccgtgaa ttattttacc aaatattatg
     5641 tcaaaattgt aagtggcaaa tgctcttttt gaggattaca atgaaaaata gttaaaaatg
     5701 gataggaagg atttaatatg gcaaaaggaa aacatcaaaa aaaacagcag atctctcttt
     5761 cttttctcgc agttttgttg ctggctgtca gcttcttttc aacaggcatc attacaggat
     5821 ggcaattaac aaaacaacct caaccacaaa tttataaaac ggaacccgca caacttgctt
     5881 ccaatttaac atctaatgag cagatgcttg ccagcaaaat tgatcaatta ctgcaaaaat
     5941 cagattatat cggttcgatt tatgtacgca aaaataatcg agtgatatta gaaaaaggat
     6001 atgggtacgc aaatctacgc ataaaagaat cgaattctcc ttctctttac tatcaaatcg
     6061 gttcgatcca aaaagcgatg actgctttac ttatcctaaa acaaattcaa tctggcaaga
     6121 taacttttga cacaaagcta tctgaatttt atcctcagat tcctggaagt cagcaaatct
     6181 caatcaaaaa tatgctttac atgcgctctg gtcttcatcg aaccgcttct ccaaaaaaac
     6241 ctatgtcaga tagtgagatc attcagtttg cccttcatca tttgaaattt actggttatg
     6301 atacgtatca ttatgaacca ttaaattata cattgcttac aggggtattg atcaaactaa
     6361 tgaatcagcc ttatgaaact cttttaaaaa aagaaatcat tcggccactc catctagaac
     6421 atacagattt ttatgaaaat gtaaaaaatt cccctcagca tgctgtatct taccaaatgt
     6481 ctgcaactga cgattattca aaagcattgg tcgagcctga aacagacatc cgaaatgaat
     6541 taggcacagg aaatataagt atgtctgttt atgacttgaa caaattcttt accagtgttc
     6601 tgtctggcga tattatacca aaagaactac ttttctcatt gtggcaaaat aatggtgata
     6661 aacatcctta cagcggcggt gtttacagtg gaaatgatta tatccttgct cagggaaata
     6721 tcaatcgatt ccatgcagta gcggcgatga aaaaagacac aaaagatgca atcgtcatgg
     6781 aaagtaatat ccaatctgat aaaaacatca aattgccggc cactgattta agaaatcaga
     6841 tttacgaact gatggaaggt gttaatttaa aaagctaagt cgagacaaaa aaaccagcag
     6901 gatgctggtt ttttgtttca atcgatttct gtctttaaat aacgttccat tccgttcaat
     6961 atatcaaatt tgggttgata acctaaagaa cggatctttg aaatatccgc taaagaatca
     7021 cgaatatccc ctgaacgctc cgcttcatat ttaagcgcta gctctgttcc taagatttga
     7081 tcaattgaat gaatcaattc aagtagcgta gtgctttttc ctgtaccgac attgaattgc
     7141 tgtcctaaag ctttttcttc cttagcaaca agtaaaagtg cttgtataac atcttctata
     7201 taaacgaagt ctctagattg gctaccatca ccaaataaag taaattctgt tttttctcct
     7261 gccaattgtt ttttgtatcg gtccactaga atcgaaataa ctccgctata tggagagtta
     7321 ggattttgat ttggaccata aacgttaaaa aatcggacag cactagtcgg aacatcatat
     7381 aaatgacagt aatccaaaac atattgttct gctgcaaact tatcgatggc atatggtgtt
     7441 aaaggacgga tgaccgattc ttcttttttt ggcaacgttg gttcatcgcc ataaactgca
     7501 gctgatgaac taaataccaa tcgttttaaa ccagattgat attttcgtac caattcaagc
     7561 aataaaagcg cactgtcaaa atttacacga tgagtttcta gaggacgttc tacagaatct
     7621 gccacactag ctacagcagc tagatggaaa atataatcga aatggtattc tttcatgacc
     7681 ttttccatca aatttttgtc agcgacatcc ccttcaataa acgtgatgtg cttcgattca
     7741 tccagattct cttttttccc catcgataaa tcatcgatca caactacatt gttttctttt
     7801 cctagataat tcgcgagcgt cgagccaatg aagccggcgc caccggtaat caagtaattc
     7861 aacatttttt ctcctctttt caagataaaa tttattacta aaaatagcta gaattatttt
     7921 tgctattaac tcttcctaaa aatagccaaa aaacaaacgt tcctacagtg ttttcataga
     7981 ctaattcatt gttaagagct gcgtatagtg cataagataa aataactaaa cagtaaacaa
     8041 aatgataatc tttaccaatg catttattat agatgattcg ataaacattc agcacaaaac
     8101 tctttattaa aaaagccatg aaaggaatta ttcctagaat acctgtcgat gttaatacat
     8161 tcaagtatgc attatgaatg gcaattccta ctttcgcaat atatccatct ggaataattg
     8221 actgtgcata agatactaca tttttcggag agactccgat aagccaactt gttttaaata
     8281 tttcccatgc acttttccat atagtaattc tagcattaga aatatctgta ttttcagcta
     8341 cgtctttcct aacaagagta ttatcaggtg tttgagtatt attaatatgt tgactattat
     8401 tcattaattc tttatgacgc tctgtaaaat taaaaaaatt tatttctttt aaatgaggta
     8461 ataattcttg aaaaagtatc ttagttgcat taaacaaagc gaataaaatc agaacagaac
     8521 caatagaaga tactatagca aggatatagt tttttaaatc tttttttgtt ccatttcgtt
     8581 ttaacacaac aataaaagat gatatggcaa ctattattat acttaccaca taggtagatc
     8641 gtgacccact taatactata taaataaatt gtaatggaag attaattaaa aaaatgattt
     8701 tatttaatcc tgtcactttt ttattataaa gataaaataa gattaggaca ataacaacta
     8761 aagatgctat agcagcattg ttaatatcag aaaacgtccc aaacagtctt gattctaaaa
     8821 aaccaattct gatcctatgt ctagcattat aatatttctc atagccgaat ttaccaaaga
     8881 aaagaaccat tgacattgat actaaaacaa accaactata aataaatccc caacttatcc
     8941 ttttaaaaaa tcgtttatta tcgctattct caaaagcaaa ctgataaatc aaaaaatact
     9001 gtaagattgc ccacattata gtctttaagt tttctcctag accatattct ttatgaacaa
     9061 aactcgttat tactaaaatc accatatatg ctatcaataa aatgttataa gttacttttc
     9121 ttttattaaa tttaattatg aaatctatcc ctaatagcaa aactcctgaa attcctataa
     9181 gtttaaaaaa aatact
//
LOCUS       DACESX010000103         9160 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.103, whole genome shotgun sequence.
ACCESSION   DACESX010000103 DACESX010000000
VERSION     DACESX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.103"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(104..928)
                     /locus_tag="IXF88_002091"
     CDS             complement(104..928)
                     /locus_tag="IXF88_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1508810.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(901..1581)
                     /locus_tag="IXF88_002092"
     CDS             complement(901..1581)
                     /locus_tag="IXF88_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508811.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1604..2542)
                     /locus_tag="IXF88_002093"
     CDS             complement(1604..2542)
                     /locus_tag="IXF88_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1508812.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2787..3638)
                     /locus_tag="IXF88_002094"
     CDS             complement(2787..3638)
                     /locus_tag="IXF88_002094"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1508813.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3792..6365)
                     /gene="mprF"
                     /locus_tag="IXF88_002095"
     CDS             complement(3792..6365)
                     /gene="mprF"
                     /locus_tag="IXF88_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1508814.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6506..7312
                     /gene="proC"
                     /locus_tag="IXF88_002096"
     CDS             6506..7312
                     /gene="proC"
                     /locus_tag="IXF88_002096"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1508815.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7391..8947)
                     /gene="rny"
                     /locus_tag="IXF88_002097"
     CDS             complement(7391..8947)
                     /gene="rny"
                     /locus_tag="IXF88_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1508816.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         2710 a         2018 c         1575 g         2857 t
ORIGIN      
        1 cgagcatttt atgaaaaaat aagaaaatag ttcggtattt attaaaataa aaagaagcct
       61 ataaaaaatt tttcgttttt ataaggcttc ttttctttat aatttagtta ttttttcttc
      121 tgaaggcaac caatattcgt ttgatgatat ttacaatcag gaacaaagca ataaagacta
      181 atgtgattgt tgctcctgga ggtgtatcta gataaaacga gctagttagt cctgacagca
      241 tccctagaaa tcctacacaa acactgatag caatcacgac attgaagctt ttgcctaatc
      301 gcataccgat tgctgcggga agaaccatga ttgcggatat caaaagtgca ccggcaatcg
      361 ggatcatcac agcaatagcc acaccagtaa tcacattgaa catcatcgac atcaaatgaa
      421 cgggtagccc atccacgtgg gcggtatctt catcaaaagt cagaacatac atcggccgtt
      481 tgaataaaaa gaacaatata ccgatggcaa cgaataagat tcctaaaaag atgacttgct
      541 cccatgtaat ggtcacgatc gaaccaaaca gataggattg gatacttgtt gctgaattcc
      601 ctccgctcaa attcatcaat actaacgcta gcgccaaacc accagccatt aaaatggcaa
      661 tggagatttc tgaataggtt cggtagatcg ttcgcagata ctccaatatc acagctgcta
      721 atacaacgac tagcatggtc gtaagtgtag gattaaggtt caaaaagaat cctaacgcta
      781 cccctgctaa agagacatga gaaagcgtat cagccatcag cgattgtctt ctgatcacta
      841 aaaacacacc tagcatcggg gcgattccgg caatgaagat agcggcaaga aacgctcttc
      901 tcatgaattc atatgaaagc aacgccattg agaatcctcc tttctcacca gtcggatctg
      961 tctgtccgca tactgtttga tatcttcatg atcatgggtg atcattagaa ctgatttccc
     1021 atggacatgc gcactatgtc ggagaagacg ataaaattca tttctggatt cttcatccat
     1081 acctgttgtt ggttcatcaa gaacaaataa gtcagggtca gtagcaaaaa tacgcgctaa
     1141 actgattcgc tgcttttgtc cgccagacaa ttcacctatt cgccgatttc gcatatccca
     1201 catccctaca gcttctaaag cacgctgtac atgttgatga tctttttcag agagacgctt
     1261 gaaccagcgg cctcttggat atcttcctga acggactaat tcgatcaccg tactaggaaa
     1321 acctgcattg aaagatgcaa cttgttgagg aatataaccg atacttaatt tttttccttc
     1381 ttgatttttc tcagcaatct ttatcattcc ttttgaaggt ttcagtaatc ctaacgatgc
     1441 tttcaccaag gttgacttgg ctgctccatt ttctccagtt aaaatgacaa attcacccgc
     1501 atctacatga taagagacat cttctagtac aggttcatca tcatagtaaa aggtcaaatc
     1561 agatatttcg atataacgca ccttacaacc tccattctaa aacttaattg atactttctt
     1621 ttaaagcttt cagatttttt tccatgacag aaatataatt ttcgcctgct ttgatttgtt
     1681 cattagtcaa gctttctaaa ggattcaaca cttctagctt cactcctgtt tcattcgaca
     1741 atgtctcagc taccttcgaa gaggcatttt cttcaaaata aatcactttg atgttgtttt
     1801 tcttcacaaa ttcttttagc tcagctaagc gacttggtgt gggttcctga tctggtgaaa
     1861 cgcccgaaat cgctacttgt tttaagtcgt attgattcgc taaataagca aaagcagcat
     1921 gctgagtgac aaatgtgcgg tttgttgcat cttttagctc atcggagtat ttttgatcca
     1981 gtgcttcaag ctttttgata taagcatcgg cattcttcgt aaaagtttcc tttttatctg
     2041 gatatttttc gcctaattct tcagcaatcg ttctgacttc ttttatagct aataccggat
     2101 cgagccaaac atgtggatcg tattcatgtg aatgaccatg cccttcttct gattcttcat
     2161 gttcgtctgc ttcttgcgat tctaaacggt cgattccttt agctgcttcg atcatcaagg
     2221 tctgtttgct atccactgaa tcctttaacg aatccacaaa aaattccatg tctgaactat
     2281 tatacacgaa aacatctgca tcggaaattt ctgctctttc ttttgcactt ggttcgaaat
     2341 catgcggctc cgttccagca gggatcaaaa gtttcacgtt tccttcatcc cctacgatct
     2401 cttttgtaaa atcatacatc ggataaaaag tcgtaacgat cgtcaaatcc tcttttttgt
     2461 ctgcttcctt gttcgtattt ccacatgctg caaacagtaa agcacctatc attaccgtag
     2521 ctgtcaaaag atattttttc atttatatgc tcctttcaat gaacaaatcg gaattttacg
     2581 atttattaac gtgaactaat gtatcaaaat aaaaaaacac agtcaataga aatccatcaa
     2641 tttctgattt aagcatgtat attcttgatt aaatcgtaaa atggatgagt tgctgctcat
     2701 atctagccat ttcactacaa taaaaaactg aagaatacca ttttaggatt cctcagtttt
     2761 tcatcaccta ttttacttct tatattttat tgcagggttc gtactaaata aacttcctca
     2821 caaaaacctt ttaacccatt atatactctc tgcgctcttg aataatgttg acacaaggca
     2881 aaaacggttg gtccgcttcc actcatcaat gcagcatctg ctccgtactt gatcatacgc
     2941 tctttgattt gatggatgac tggatgccgc tttcccgtta tatcttctaa actgtttccc
     3001 atttttttga tcatctgctg ataatcttgc tctcggatgg cttcagcaag tccgtggatg
     3061 tcttgatgtt ggatggcttg cgtatctact tctctaaaca ctgtacgtgt agagacactc
     3121 attcttggtt tgaccaacac aacccagcac tgtggcatag aaggtaatac ttggatttct
     3181 tcccctttgc caccgacaaa tgccgtactt ccgtagacac agtaaggaac atctgtaccg
     3241 acttccaaac cgatttctgc cagttcttct aacgaacagc ccagattcca taattggttg
     3301 atccctctta gagcagctgc acagtctgta cttcctccgc ctaatccagc tgctactgga
     3361 atattcttct taacaaagat cttgacaccg gttttgatat tgtatttatt ttttagcaat
     3421 aaagcagctt gataaacatt gttccgttca tctaccggta aaaatgcttt atttgtttct
     3481 acaatgattt tgtcttctgt tatttcttca aaaaatagat gatctgccaa gtctacgctc
     3541 gacatcacca tttccaactc atgataccca tcttgccgtc tatataatac atccagtcct
     3601 aaatttatct tggctggcgc tttttcaatc aattccatcc tctcacccat tcgaaaagtc
     3661 taagtcgcaa gtattctatc atagggaaaa ataaaagtat agcttatgtc tgcctagtgg
     3721 ttgattttgt catcgtttca acagctttca cagaattttc tcttcataat ccaaacaagt
     3781 tctagtgatt cttataattc tgtgagcact tcttctgcca aactcggaac atttcctttt
     3841 tcttttccta tcagactgag tagctgcatg gtagcaaatc cgaaattact tcctttccta
     3901 taagcaaaat aacgtccttc ccaatgatcg acatatttta atttcttttc ataagcagac
     3961 tggaaggaat agaaaaaatt accataatta tatacgatat gcatgacttt ttcttgaaag
     4021 aaagaaaaat ctgtttctcc aacattagct aatggagcta agcctagatt ggcttccgag
     4081 tagcccattt tctcgtatac aataaacaga ttggctaaca gatagtcagc taattcctca
     4141 gattctttag gtaagacccg caacaaatca tatcccgcct gcttcttcgt catcggctga
     4201 gcggtgatga agccaacgac tttttgatct ttacggacaa taccgacacc gctagataat
     4261 acatatgatt tatcaaatcg tcccacagag aagtattttt ctcgtgcacc ttgaagccat
     4321 ttttgtgaaa cttcatttac agcttgatgt aattcttccg gcagttgatc gtaccaagta
     4381 aaatggtatc cttctttcat caaacgttga tattcaagtt gctgactcag ccgagacaag
     4441 tcttctgttg ctagatcaac aatccctgct tcacctactt tcgtgaagtg aaaacccaat
     4501 tcatgaagca tcactacgta gctttctgat atcttataaa acgcaagctg atagcctaaa
     4561 caatctgctt gtttcataaa ctgattcgtt gctgcccgca tcattttagg attgccaact
     4621 ggatctccta aaacaaagca tttgtttcct ttgatctgat aaccaaaaac gacttgatct
     4681 tggtcgtctt cttggtaata aaaataactg taacctgaca agcgaagctg atggcttgta
     4741 gctgtgcctc cgtatgtttc caataaacgg tcaaaacggt ctttttcgta ctcactgcca
     4801 agcttcgtag tatcttcagc taaatattga taaagcgcca ctaaagctag caatgagatg
     4861 cccaaaccaa taagccctga gaaccacaca tcatctgaag gaaacaaaat aaaatgattt
     4921 cctgttattc gaccgactaa aaagcgtccg ctgtgatagc ctgcaattgc gtaaacgata
     4981 aacaaaaagc cgaacaagct agcatcaaag attatagctc cccaagaata aacgaatttt
     5041 gttcgataaa attcttttct ggataaccac acagctaaca gaatcaagac atataccaat
     5101 atcaactgcc aagataacgt acgtgtgatt gtatttaaaa taccaaaaga tagtaaagca
     5161 atcgtaggca gaaatgcctt tttgactttt actgaaagtg ctctagcaag gccgagtaat
     5221 aaaaagccaa ccaatagatt cactgtctga tctaaaaaat tgaatgagaa cggcaataat
     5281 acttgaaata gccgactgat tgcagacaga ttagtgattg ttgatagaag taccatcatg
     5341 atccccgcaa aatagacagc acttacgagc acgatatgcg caaccttttg gaaaaacagt
     5401 cgcggaatgt tatctaaaaa tcgattgatt ttgactcctg tatgtgtgat gaacaataga
     5461 agtcccgtca taaaaggtag aacataataa aacaaccgat aatataatag ccaaactact
     5521 gctacatctt gccgaacaga caattgactc attcctaaga tcatcaaaac gtcaaaagtc
     5581 cccattccac ctgggaccat cgtcagcatt cctatcaatg tagcaatcat gaacatagga
     5641 tagatcgaaa gcatcgaaat atctattctc atcaacttgc cgatgatcaa aaataccgca
     5701 agggctcccg tccattgtcc aaaggatgcc agtagcagac caagcaagcc ttttggataa
     5761 aactcgaaga acaatgtctt tctctttaga taagcaaaaa agaaaatgac tggtgctaaa
     5821 aaactaccgc cgaaaagcca gatccaatag cttcgaaaaa tactttctga ctgaataaaa
     5881 aagacatcaa taaatgtaag aaacgaccat acagacaaac cagtcagcat aaatagtgcg
     5941 acttttgata cagtggctaa aaccattttt ctatttgtct gctttccata aaaactagcc
     6001 cgcagacttg ctcctaccac accaccaaaa ccggcgagat tgttgatcgt gtttgttacc
     6061 cacgcagcaa gaagaaactc ttttctttcc atttttggtt ttccttgttt ttctaagact
     6121 ctgatcgtca cccaatcata caacaacatc ggagtgacac ccagcagtcc agccagaatc
     6181 atgagcagga tcgtcatgcg gctttgttgt cccatccttt gccaaatatc ttgccaactc
     6241 atgccattag caatatttgc gacttgattt gccacaaaga ctaacacgga gccgaaaaaa
     6301 atcagtttta aaatcagact atgttccttt atccattgca gcatcaagta tcgtttattt
     6361 ttcacatttt cccattccta acttgtatac ttttgtgtca taccccaatg ttatcttatt
     6421 tattttaatt tttccacttt agacctgttc tttgcttgcg atgatagaaa aagaaagata
     6481 tcctaatgct aaaggagagg ataacatgaa agtaggattt atcggcacag ggaatatggc
     6541 acaagctatt attgatggtt tattaaaaaa aggaacagtc tcgaaagaag aggtgttcgt
     6601 ttccagtgga cattttgaaa atgcacaaaa atttgctgct gaaactggcg tccatgcatg
     6661 caaaagtaat caggaagttg catcacaatc agatatcgtt cttttagccg tgaaaccaaa
     6721 aatcattgaa aaagtattgg aagaactaag agaatatagc cagaaagtct tattcatttc
     6781 tattgcttca ggaaaatcat taacagattt agaaacagcg cttgaagatc ctcaggcctc
     6841 catcgtacgt gtgatgccga atgtcaatgt cagcgtagga gcaggcgttt ctgcgatttg
     6901 caaaaatgaa gctgccacgg aggaacagct tcagcaagct acgatgattt ttgaagcaat
     6961 cggcagcgtc tatcctttag cagaatcaga tttcagtaca ttcattggcc ttgctggcag
     7021 ctcacccgca ttcgtttaca tgctgatcga tgcgatggga cggactggtg tactgaatgg
     7081 tttgtccaaa cagacagcca ctgagattgc tgcaaaagca attatgggtt cttgtcaaaa
     7141 attcctttca agtgataaaa atccatggga acttgtagac caagtttctt ctcctggcgg
     7201 tacaactgtt gcaggtgtag ttgccttaga agaagcagga ttcatcccag caatcatcaa
     7261 aggaatcaat acgacgatcg agaaagatct tgagatgttg aataaaaaat aatgatagca
     7321 atgttttttc gtgccttaca aaaaaagaag agctgtggct tttgccgcgg ctcttctttt
     7381 gtatttttta ttatttagca taatcagttg cacgagtttc ccgaataacg attactttga
     7441 tgtgtccagg ataatccagt tcttcttcga ttcgtttacg aatatcacgt actaaacgaa
     7501 cagcgtctaa atccgagatt tcttctggct tgaccattac ccgaacttcg cgtccagctt
     7561 gaactgcgta acttgattct acaccggcga agctgttcga aatattttcc agacttctta
     7621 aacgacggat gtagttttct aatgattcac ttcgcgcacc aggtcttgct gctgagagcg
     7681 catctgctgc agctacaagg acagagataa cagaagtagc ttccacatct ccatgatgag
     7741 aagcgatcgc gttgattact actgggtttt ctttgtactt ggcagctaat tccgcaccga
     7801 tctcaacgtg agatccttca atctcatggt caagtgcttt cccaatatca tggagcaacc
     7861 cggcacgttt tgctaattgg atatcttcgc caagctctgc tgctaagata ccagctaatt
     7921 ttgcaacctc gaccgaatgc ttcaagacgt tttgtccata actagttctg aatcgcagac
     7981 gtccgatgat cttgattaga tctggatgaa gtgtatgagc accgacttca aacgctgcct
     8041 cttccccgta ttcgcgaata cgttcatcca tttctttacg agatttctcg accatttctt
     8101 cgatacgcgc tggatggatt cgtccatctt gtatcagttt ttccaaggtc atccgtgcga
     8161 tttctcttcg gattggatcg aatccggaaa gaacaactgc ttccggtgta tcatcgatga
     8221 ttaaatcgat accagtcagc gtctcaagcg ttcgaatatt acgaccttct cgtccaatga
     8281 tacgaccttt catttcatca ttcggaaggg taacgactga aacagtcgtt tctgaaactt
     8341 gatctgctgc gcagcgttga atcgctaatg ataataagtt tttcgctttg cgatctgctt
     8401 cttctttggc acgctgttca gattctttta ccattagagt taactcatga ttcagctctt
     8461 cttcagtcga tttcatgatg acatctcgtg cgtcctcttt tgataaacca gcaatttttt
     8521 ctagttcttt ttgttgatct tcaatcattt tttcaacgtc tttttctcgc tcttcaatta
     8581 attgctgttt tgaactaagt gcttcttctt tactttcaag tgagttctcg cgtttttcta
     8641 acgagtcatc tttacgattc agcgtttgtt cgcgttgaat caaacgattc tcttgagatt
     8701 ttaattctaa cttactttct ctcagctcac tttcaacttc agaacggtat ttctgatttt
     8761 cttccttagc ttctaacaat gcttcttttt ttagagtttc tgcttctttt tttgcactat
     8821 cgatgattcc agcagctgat gattgtgcac cagctatttc tttctcgtga cgtgactttg
     8881 cgaccataaa ccctaaacca agaccgacaa ttaaaccgat gatagcgagg agaatgttga
     8941 ataccataaa atccacctcc atactatctt tttcattttt atcatctata cagcttctat
     9001 gataaattat tctaaactcg tcaatatgca tcttcgcata tatgtgtaca aaaaacatgc
     9061 acaactttat tctaatgttt gtaccccgta gtgtcaactt taaataaaca aagaaacggc
     9121 tttctactta ttcattaaac gatggttttt aaaataaatg
//
LOCUS       DACESX010000104         9083 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.104, whole genome shotgun sequence.
ACCESSION   DACESX010000104 DACESX010000000
VERSION     DACESX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.104"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..498
                     /locus_tag="IXF88_002098"
     CDS             <1..498
                     /locus_tag="IXF88_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1508817.1"
                     /translation="HSAAIFEKPQIQQLTNIVSPAVRGVFLKFLEKAESEKIPIQVVI
                     PVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITINLLEEKEGSRFSIANKANPT
                     NLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELKIEFSKQNHYFFVEMYLASRK
                     SKSAY"
     gene            820..1047
                     /locus_tag="IXF88_002099"
     CDS             820..1047
                     /locus_tag="IXF88_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1508818.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            1170..1496
                     /locus_tag="IXF88_002100"
     CDS             1170..1496
                     /locus_tag="IXF88_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1508819.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            1564..2277
                     /locus_tag="IXF88_002101"
     CDS             1564..2277
                     /locus_tag="IXF88_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1508820.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            2587..3438
                     /locus_tag="IXF88_002102"
     CDS             2587..3438
                     /locus_tag="IXF88_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1508821.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            3559..5424
                     /locus_tag="IXF88_002103"
     CDS             3559..5424
                     /locus_tag="IXF88_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1508822.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            5438..6880
                     /locus_tag="IXF88_002104"
     CDS             5438..6880
                     /locus_tag="IXF88_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1508823.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            6988..7689
                     /locus_tag="IXF88_002105"
     CDS             6988..7689
                     /locus_tag="IXF88_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1508824.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            7861..8340
                     /locus_tag="IXF88_002106"
                     /pseudo
     CDS             7861..8340
                     /locus_tag="IXF88_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            8351..8857
                     /locus_tag="IXF88_002107"
     CDS             8351..8857
                     /locus_tag="IXF88_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAQ1508825.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
BASE COUNT         3049 a         1500 c         2008 g         2526 t
ORIGIN      
        1 cattctgcag ctatttttga aaaaccgcaa atacaacagc taacaaatat tgtatcccca
       61 gctgtcaggg gtgtcttttt aaaatttcta gaaaaagcag aatcggagaa aatcccgatc
      121 caagtagtta ttcctgtcga agtgaaaaca atcccgattg atctggttgt ttttattcgc
      181 tgtttatcta tttttatttc taacgcattc gatcatcgtg tagccgatca gagtccgatt
      241 acgatcaatc tgctggaaga aaaagaggga tcacgtttta gtatagcgaa caaggcaaat
      301 ccaacgaatt tgacgctatc ggagatggtc aaaagaggaa aaacctctaa aaaaaatggc
      361 gggttaggtc tatacagtgc gaaaaaaatg ttggatgttt acgaaaatgc agaactaaaa
      421 atcgaatttt caaaacagaa tcactatttt tttgtagaaa tgtatctggc atctagaaag
      481 agcaaatcag cctactgact tactgtcaaa ggataagttt gaggaaagtc tacacggaga
      541 tagagacaac aacacagcat attcaaaaga aacagaagcg tgatgaccgt tgtcaccctt
      601 ctgtttcttt tgctatgtag ttaaacaaag tagttgacac ttccgctact tagtattagt
      661 acgaaagtat acttcatgat actaagtaga taagaagtga aaagcggaac ccacgatcgt
      721 caaaatgcta actgctttgc ttacaccaga taaagggaag agcaatatcg cttattttag
      781 gcgtggagaa gcaagcaaat ccctacgatc acaaaaaaaa tgctgaccca gcagttaaga
      841 gagttagaac aagatgaaag tatccatcga aaagcctata accaagtccc gcctaaagta
      901 gagtactcgc tcaccgaaga aggaaaatca ttaagagaga ttctagtagc gttgtctgtt
      961 tgggaagaag aacgtatcga aaaacaaaaa gcctcaaggg aagaagacac gctactgcat
     1021 acacatggtg gctacctaaa ttattgattt tctatgacat ttattttaaa taaaagaaaa
     1081 caggagtttt tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa
     1141 gtaagaagtg attggaagaa ggcaagcaaa tgaatagtat ggaaaaagtc aaccagttta
     1201 gagatgaacg taattggcga caatttcata acgaaaaaga tttggctatc tctatttctt
     1261 tggaagcatc tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa
     1321 cacaattaga acgaatcaaa gaagaactgg cagatgtatt gatctattca tacatgatgg
     1381 cagacaacct tcacctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt
     1441 tgaaatatcc tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttgtagaaat
     1501 gtgtcattcg gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag
     1561 aaaatgaaaa aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg
     1621 ttattagtag gactagcttt gattttcaat gaacagatca aggattattt tgtcagagag
     1681 acaggagata aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat
     1741 aaagatgtca gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg
     1801 tcgcaaatga gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca
     1861 atcaatttac cggttttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca
     1921 ttatctccag atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg
     1981 aaccctgaat tgctgtttac accgctagaa aatttagaga tgggtgccaa gatctatttg
     2041 acagatttag aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca
     2101 gatacccaat tactgaatga agtcgaggga aaagaaattg tcacactaat tacttgtggc
     2161 gacatggacg ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacatcgata
     2221 aaagatgcga cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat
     2281 actgggacat aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa
     2341 accaatttgc tgaaaaaaag agaaacgcaa agaggtgcat ctctgatttg gttgttttct
     2401 agatagactt gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa
     2461 ttcgatggta tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca
     2521 gaattcagct aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga
     2581 agcaaaatga aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc
     2641 ggggaagaag tcgtttgtat gggaaaagga ctagcttttc aaaaaaagat tggggacgag
     2701 atttcgccag aagcggtcca aaaagaattt gttttaagag attcttttgc taaaaaccaa
     2761 ttccagcagc tgatggctga tattccggca gaggagatcg aatgggtcaa acaagtagtc
     2821 gaactggctg aagataaact aaaagttgaa ttttcgccta atatttatct gacattgacc
     2881 gatcatcttc attatgcgat cacacgagca aaagaaaata tccaactgcc taatccatta
     2941 ttatttgaaa caaaaaaatt ttatccgaaa gaatatcaag cagcgaaaga ggctcttttg
     3001 ttcgtgaaac aaaagacggg tgtggaactt tctgaggatg aagccggttt tattgccttt
     3061 cattttgtga atagccaaca aggaaacgaa gacatgcaag tgacgatgac cgctaccaca
     3121 atggtaagag atatactgag tatcatcagt aaattcttcg gcatcgtgtt cgatgaagaa
     3181 tctttgaatt atcagcgtat cattactcat ctgcagtttt ttgcccagcg ttatctcaaa
     3241 ggggaaggat ctgacgagca agatgaattt ctctacgcat tagttcaagg aaagtatcca
     3301 aaagcgtttc attgtgtcga acggatcaat gagtatcttc gacaaacgca aaaacaggaa
     3361 atggggatcg cagagcagat ttatttaacg attcatattc aaagagtagt aagtgagaaa
     3421 aaaagcatcc aatcataagg attgttactg tatggcaggc aaaacctgga ttgaaaaaat
     3481 aggttcacga atgtttgtga ctgttttttt caatctgggt ttttattttt taaataaaaa
     3541 agaaagaagg aaaaaacaat ggataatcaa gcagttggtc gtcgtgtatg ggaagcggta
     3601 ggaggacaaa agaatgtcaa aagtttagta cattgtgcta cgcggttgcg attcagatta
     3661 aaagatgaat ctttagcaga tacgcaaaag ttaaaagaag acccggacgt gatccaagtc
     3721 gtacaaagtg gcggtcagta tcaagtagtg attggcagta atgtagcaga tgtttatcaa
     3781 tcgatcgtgg atgaggaagg tcttgcagtt tcggaggatg gaaaagaaga gtccaaaaat
     3841 attttgaatc gtctgatcga tattatctct agtatattta caccttttct aggggcgatg
     3901 gcagccgccg gggtattgaa aggattctta tcattggcaa cggtgatggg ctggctgaca
     3961 gctgatagcg gtacttatca aatattgttt gctgcagcag atggtgtctt taccttctta
     4021 cctgtcatgc ttgcttttac agcagctaaa aagttcaaag ccaaccagtt tttagcagta
     4081 gcaattgcta tggcacttgt gtatccagct atcacagcag ttgctggtgc gggagaagct
     4141 atcagtttct ttgggatccc agtgattctt tcaccatctg gttatacttc ttctgtcata
     4201 ccaatcattc tagccgtatg ggttcaaagt aaattagagc cattcgtaaa aaaagtgatt
     4261 ccgcaatttc tgcaaatgat tttagttccg cttgtcgttt tagtagtgat ggttccatta
     4321 acatttcttg ctttaggacc aatcgggacg gttgccggta atgctttagg cggtctcttc
     4381 aactcgattt atggatttag tccaatcgta gcaggtctaa tcatgggaag tttatggcaa
     4441 gtattcgtga tgttcggcat gcattgggga ttcgtaccga tcatgttttt gaatatcgaa
     4501 caatatgggt ttgatgtatt gatgccaatg cttcttccag ccatcttggc acaaggtggg
     4561 gctgctttag ctgtagcatt gcgaacaaaa gatacgaaac ttcgagcatt agggatttct
     4621 tctacagtga cttcgttatt tggaatcact gaaccaactg tgtatggtgt gacattacct
     4681 ttgaagaaac cattcattgc cgcttgtatt tctggaggta ttggtggagc tattatcgga
     4741 ttttccggag taaaagcttt ctccagcagt ttggtcagct tattgacgat tccgactttc
     4801 attaatacgg tggatggtgt agagtcaaat gtgacagtag cagtaatagc tacaggaatt
     4861 gcttttgttc ttgcattcgt aggtacattg attttaggat ttgacgaaca aacacaagac
     4921 aatcaattag aaaacaaaca tgcgaatgca ggagaaccga ttacttcagc acggcatacc
     4981 ttaaaaagtc ctttaacggg taaagtactg ccattgagtg aagtaccaga ccaagttttt
     5041 tcttctggcg tgatgggaaa aggtatcgct attgatccag aagtaggaga actagttgca
     5101 cctgcagacg gagagatcac gactattttt ccaactggac atgctgtagg gatcacaacg
     5161 actgacggag cggagatttt gattcatatc gggatggata cagtagaatt aaatgggaat
     5221 ggctttgaga tcttagtcaa acaaggggat ctcgtcaaag caggagattt attgattcgt
     5281 tttgacatcg aagcaattag agcagctggc tatagcgtaa tcactccagt tgtcattacg
     5341 aatactgatg catttgccga tatacttgaa ttagaccaaa aagagattat tgcaaacgaa
     5401 gatgttctgg caattgtaaa ataaggagga aatcaggatg caatcacgtt tttcagaaaa
     5461 atttttatgg ggtggagcaa ctgctgccaa tcaattagaa ggggcttatg atcgagacgg
     5521 aaaagggcta tccgttgcag atgctatgcc tggaggtaag cagcgctttg cgattatcgg
     5581 cagtgaagaa tttgactgga cgattgatca agaaaaatat atttacccga atcatcgagg
     5641 aattgatcat tacgatcgtt tcaaagaaga tcttgccttg tttgcggaaa tgggattcaa
     5701 atgttatcgt ttttcgattg cctggacacg aatttttcca aatggagacg aaggtactcc
     5761 taacgaagcg ggattagaat tttatgatca gctgattgat gagtgcttaa aatatgatat
     5821 tgaacctgtg atcacgattt ctcattacga aatgcctctt catttggcaa aagaatacgg
     5881 tggctggaaa aatcgaaaac tgatcgactt ctatgaacga tttgctcaga cagtattaga
     5941 gagatacagc agtaaagtca aatattggat gactttcaat gaaatcaatt cggcctttca
     6001 ctttccagct ttaagtcaag gattagtaaa aagcaatgga gcaggagagt accaaaatat
     6061 tttccaagca tggcataatc aattcgttgc aagtagcaaa gccgtaaaaa tcggccacga
     6121 attgcgttcg gatatccaaa tcggctgcat gattatctat gcaacaactt acagcatcga
     6181 tgccaatcca gtcaatcagg cagcaacaat gatccaaaac caagaattca attttttctg
     6241 cacggatgtc caagtacgtg gagaataccc tgcatacaca gcgcgtaccc ataaaaaata
     6301 tggagtcgat ccggaaaaat tagagcaaac ggaaaaagac ttccagttgc taaaagaata
     6361 tccagtggac tacatcggat ttagttatta tatgtctacg gcaatcaatg agactgatcc
     6421 acaagcagca acttcagaag ggaacttgct gggaggagta aaaaatccgt ttcttgaggc
     6481 aagtgaatgg ggctggcaga tcgatccaga agggctgaga atcgcattga atgaattata
     6541 caatcgttac cagaaaccat tattcattgt tgaaaacggt cttggagcca ttgatcatat
     6601 ggaagaggat ggcacgatta ttgatgatta tcggatcgat tatttacgtc gtcatatcga
     6661 agcgatggct aatgcagtag cagacggtgt agatttgatg ggctatacgc cttggggatg
     6721 tatggatctt gtcagtgcat caacaggaga aatgagtaaa cgttacggct ttatctatgt
     6781 ggatctagac gatgaaggaa atgggacact gaatcgatca aagaaaaaat cttttgattg
     6841 gtataaagaa gtaattgcct caaatggcgt aaacttgtaa ttaaagcagc tgaatgaaaa
     6901 agaacacact tctcacatgt gatttgtgtt ctttttttat tttttatgtg ctattctttt
     6961 agtaatagta aagcaaaggg tgacgttatg aaaaggacac gtgtactata cttggaagtc
     7021 gcagataaaa ttaaagaaga tattttctca ggaaaatatc cagtcggttc gatgttaccg
     7081 acagaaagtg aattagaaga actgtttcaa gtcagcaaaa tcactgtccg aaaggcaatc
     7141 gaactgctgg catcagatga atatgtcgca aagaaaagtg gtcgtgggac gacggttcta
     7201 agcaatcgtc cttacaatcg attatccaaa gcggcaagct ttacgcaaat cttggagaac
     7261 tcccgatatg aagtccggaa agaaacgctg gatttgcaat tggtccatat aaagaaggaa
     7321 gatcctttgt tttccttttt cggtgagcaa gctgtttgtt ttaagcgctt atattatttg
     7381 aacaatcagc cttatattta ttttgttcat tatcttccac aagaaatgat gcaattcagc
     7441 agagaagaat ttggcaaaga atctttgtat cgtttgctga cgaaaaaagg atatactatt
     7501 gactcatttt ctgatgattt ctcggcagta tttttaacag aagaagaaca agaaattctg
     7561 aaaacagacg cacagatcgg catcaaacgg atacggaaat cgtctacgca aattggggaa
     7621 atgattgaat attcggaggc ggtctaccat acggagctgc atccttacca tattgattat
     7681 gaaacataaa taaaaaaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
     7741 ccaaacaaaa atagcttctc atattagctt ctcatatttt tcgtttggtt aggcttatta
     7801 ccgcagatac ccggctgttt ctggattacc atttattctg gataaggggg tgtggcacga
     7861 ttttcagaag gaatctatga accaatcact gtattgattt tagcttcgat ggtaacccca
     7921 ttcggcttga cgatcgctta tttcttaggg aaaatcattc gtaaaaatat attcaatcgt
     7981 caagaaatcg atacattgaa gaccgctttt cctatgggga tctgtcagat cacagaggga
     8041 tgtatcccta tcgtattgaa cgacttggca cgcaatgtca tagcaacggg agtcggtggg
     8101 gcagtcggcg gtggattgag catgttttgg ggcgcagata gccgtattcc ggcatcggga
     8161 atgtttgcag tcgctacgat gaccagacca tgggcattca ttggtgcatt gttagctggt
     8221 tcttttgcta cgggaataac tattttatta ttgaaaaaac cagttgaccc aaatgcagaa
     8281 gtcatccaag aagaaaaaga ggaagaagag atatcttggg atgacttgac aatcagttag
     8341 gaggaacaga atggattaca agaaaagagt acaacaaatg ttcctagatt caggagtagt
     8401 attgaaagaa acagaattag aaagaatcga atatacagat tttggattag gagcaatcga
     8461 gactgaagga ttgaatttat ttgtttatga aaataatgag cgatactgtg ggaaagaact
     8521 ggtattattg ccttatcaaa cttgtccaga gcatcggcac ccacctcgag atgagaatga
     8581 aggaaaaaag gaaacctttc gttgtcgaaa aggcttagta tatctttatg tcgaaggaaa
     8641 agaaacaaaa aaactttcct gtattattcc aaaggggcaa gaacgttact atacggcaaa
     8701 aaaagaaatc atacttcacg ctggagatca gtacacgatc gaacaaaata ttaaacactg
     8761 gttccaggca ggtgcaacag gcgcggttat caccgaattt tcttccagca gtgatgatcc
     8821 aagtgatagt tttacgaatc cagctattta tcgttaatac aaaggtttgg gaacaagctg
     8881 atgaaccaaa atgatcaaat ttcgattatt tggttcgtcg gcttttttat ttagagaaac
     8941 atcaggcgtt ggtgaaacat aacacgagaa taaaatggat ttttcattaa taggagaaac
     9001 aagtgtgtga ctatttaaat aatgcgtgtt attgacatgc aggacgaaat cccaatttct
     9061 tctataagta agtcgtttca att
//
LOCUS       DACESX010000105         9031 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.105, whole genome shotgun sequence.
ACCESSION   DACESX010000105 DACESX010000000
VERSION     DACESX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.105"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(107..1879)
                     /locus_tag="IXF88_002108"
     CDS             complement(107..1879)
                     /locus_tag="IXF88_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508826.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSRTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1833..3587)
                     /locus_tag="IXF88_002109"
     CDS             complement(1833..3587)
                     /locus_tag="IXF88_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508827.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3697..4266)
                     /locus_tag="IXF88_002110"
     CDS             complement(3697..4266)
                     /locus_tag="IXF88_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1508828.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4284..5708)
                     /locus_tag="IXF88_002111"
     CDS             complement(4284..5708)
                     /locus_tag="IXF88_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1508829.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(5710..6378)
                     /locus_tag="IXF88_002112"
     CDS             complement(5710..6378)
                     /locus_tag="IXF88_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1508830.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6509..7093)
                     /locus_tag="IXF88_002113"
     CDS             complement(6509..7093)
                     /locus_tag="IXF88_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1508831.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7424..7657)
                     /locus_tag="IXF88_002114"
     CDS             complement(7424..7657)
                     /locus_tag="IXF88_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1508832.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7672..8622)
                     /locus_tag="IXF88_002115"
     CDS             complement(7672..8622)
                     /locus_tag="IXF88_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1508833.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8619..>9031)
                     /locus_tag="IXF88_002116"
     CDS             complement(8619..>9031)
                     /locus_tag="IXF88_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1508834.1"
                     /translation="LVLVTVVLFFKELLITSFDPLMAKAMGMNVSFYHYLLMILLTLV
                     AVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFISATVGACSSLLGLFIGYSFNI
                     AAGSSIVLTAAFLFVISFLISPKQKFLRKKERKL"
BASE COUNT         3017 a         1653 c         1189 g         3172 t
ORIGIN      
        1 ttctttcgtg tattcaaaat tagatgctgt ttaataaaat tattcttttt gaaaaagaat
       61 tataactata tcccaaaaat tgtaggttaa tttaaagcct atttcatcat cgtgattgag
      121 cttctttttc atagacgaat ttctcagata gtttagtttt ctttatgaga tttttgtata
      181 tttctgacct tcgatacaac tcactatgag taccttgatc aactattttt ccttgatcca
      241 tcactacaat tttatttacc ttttcaatag acttcaagcg atgcgatata attagtactg
      301 tcttatcttg tattaaacga tttaaacttt cctgaatacg tttttcattt tccgcatcta
      361 aactggcagt aatttcatct aaaattaaaa ttggcgcatt tttcaaaaaa gcccgtgcta
      421 tggataggcg ttgtcgctct cctccagaaa gtgaacttcc gttttctccg accaatgtat
      481 gataaccgtc aggtagtttt tcaataaagt cttcgcagtt agctaaacga gctgcttttt
      541 tcacttcctc atctgacgct tgactatttc caatacgtat attttccaaa atacttgtat
      601 taaacaaagt tacctcttga aaaacaatag cgattttgct aaacaaagaa gctggagata
      661 cacgtttaat atcataccca tcaattaaaa tacagccttc atcataatca tacagtctag
      721 acactaattt caagatacta gttttcccgg aaccactagc acctactaac gcagtcactt
      781 ctccttgttt agccgtaaaa gagatatgat ctaaaattgg ggtattgtta tcatatgaga
      841 aagagacatc tcgcaactca atgtcaaaag atttcagagg actatctgat ccctcttgga
      901 tactggtttc cttcatagct ctaattcgct gtattttagg agctaaataa aatatttcaa
      961 gcactgcttc tttcatagaa tcgaaggaat cctttatttt tattgcggct agtaagtaac
     1021 caacgacgta gagtatagtc acttcacccg ttaacagtaa gtgaacacct actagaatga
     1081 ctactgctaa actaacatat gagaaaatag aggataaggc catgactgaa aaagtactta
     1141 attctacctt taaatgaatc ctttcactct cttccatttt tttgaaaagt ctgtcttgaa
     1201 cttttttaga tagattaaag ctattaatct cttgctgcaa ttctatagtt tcttgaaatt
     1261 cttccgagtt ttctctcaaa gtatcgtaat atttagtatt ggctttcgtt tgggatttct
     1321 ttgataacaa gattaagaca aaactaaata acgttggcaa aataacagct aatcccattt
     1381 tgacattacc aaccaaaagc atcactgaaa taaaagggaa aaacagagcc ataccgccgg
     1441 ctttaggtat tgcatgactc atcgcatgct caataccttc aacgtcagac atgatagttc
     1501 gagataaatc tgataaatta tgttttgaaa aataggataa tggtaaattt gacaaatcgt
     1561 ctgcaatttg cattcttaaa tgcgcacttt ctttataagt actattatat aagttttcat
     1621 actccctatc taacaaccaa tacatgaaaa gcaaagtaac gaacgatact gtaaaataaa
     1681 gccaaggcgg ctttacattt tctaaaacta attcgtctat cagcatcatt ataatgaatg
     1741 ctggtaccat attaatgcag tatacaaaaa agctagaaat agatgcttta accaaatctt
     1801 ctgcgccctg attagttaaa gcgaatttgt ttttataaaa acttttcatt gtcaagcctc
     1861 caatcatttg cattctcata attagtgacc aattgtttat acacttgatg tttagccatt
     1921 aattcattat gatttccacg ttctactatt ttcccttctg acaagacaag aatttcatct
     1981 acagatttaa tgctggataa tcgatgagct atcataataa ctgttttttc tttcatcaga
     2041 tgttgaaaag ccttttgtag ttcatactca ttatctggat caatagatgc actggcttca
     2101 tccataataa caatctttgc atcttttaaa attgctctgg caatagctat acgttgtttt
     2161 tctcctcctg acagataaac tcctttacta ccaattaagg tatgctctct ttcgggaaat
     2221 ttttctaaaa tcgattcgca accggctaat cttagcgcgt caaatacttt acttgctggc
     2281 gcttgaggat ttgctataga aacgttatcg taaatactct gattgaatag tttcgtatct
     2341 tgaaagacaa aagcaattgt gttaataatt gtgtcttttg aatagtgatt taaaggctta
     2401 ccaccaattt taatctgtcc cttatccaca ttgtagaaac ctgaaattag tttagctatc
     2461 gtactctttc cgcttcctga ttttcctact aatgcataaa ttcttttttg ttctaaaact
     2521 aagttgaaat tttctaaaac acactcttct ttgtatgaaa agctgacatt ttcaaatgca
     2581 atgtcacaat ttttaatctg tgtctcgttc ccatatttta gttggtcttg cttcatttct
     2641 aaataaaggg agtctagtgt ttcgactgca tatcttgctt caaacaagtg catacttaca
     2701 tacattattc tcatgaaaga aacaaaaata attccactta aaaaaaacaa catcaacaag
     2761 tcaacagcta atatcttggg agtggtaaga gaagttataa aaaaggttat aggaactatc
     2821 aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa caaaaggtgt tttcccactc
     2881 aaagaatact tatatgcaaa cttagcataa tcgttaattg ctttattcaa ggctttaaat
     2941 gatgttactt tagcaccaaa tatctttata acttgaattc ctcggatata ttctaccgtt
     3001 tcagagctta acacatctaa cgattcttga tatttttgaa taaaatttgt attctctgtc
     3061 attttcttta acaagaatac accaattact aacatggcga ttagaaccaa tccaatttta
     3121 aaattaatga aaaagcctaa ggcaactgat aaaacaggtg ttactatggc ttgagcacta
     3181 tctggaatca agtgagcaat tgccatatgt gttttagccg cgttgtcatc aattgtcttt
     3241 cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac ttgcttctgt taaaccgcta
     3301 attccctttt ttctcaaatt agtctctaag cgaaacccaa ggatatgaga aaataagcca
     3361 gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa cagtttgaac cgcataactc
     3421 ttagctaaat attcatctcc tacaataatt agagacttaa gaaatttgta aatactgtaa
     3481 tatccataga ctgttaatac gacagaaaaa tcagaaaata aagtggctaa ccatccgtag
     3541 tatttcattt tagggacata actaaataat tttttgtata ctttcatttt ttcctccccc
     3601 gcattcaatt tcttctctta ttcgtttaca actgttaatg ctttccgtaa tagcgtaaat
     3661 tagagatgta caaacagata gaaggtaaac tacgttctat tttttgtatt tttttaagac
     3721 ttttcttcca atcaatacac caatcataga taaacttgct acaactaaca ctgaaatgat
     3781 tccccatata cctaccgcat agtggatcat tgcatccatt gttgcagcag attgtccttt
     3841 atctatccaa tcttgacgaa aacgatctgt aaagaaccat ataggaatcc actgtccggc
     3901 agcactgcct aattgcatta gagcccaaga cgcaacttga ggaattgctc gatcatatcc
     3961 aaatacagat agaataatat cagctaagat cgccatacta atcgaactga taaaccaagg
     4021 taagtacgcc ccgcccatta acgtaaaaaa taacaataaa acaattgtgt aaatagtaaa
     4081 aattcctttt ctagggtagt tatagctaat ataaacaaat atgattccag ataataatcc
     4141 aaaaatccct ggggatatgc tgtgaccaaa cgccccacca atactactaa ccattattga
     4201 aaaaaagtaa attactaggg aaataactgt agctaaacca ataaaaatat aatcttttgt
     4261 tttcatatat attctcctca attctatttc gttatgtcat taaaaatctg ttctatcaaa
     4321 gttgaatctt ctactctaag ttttatatct ttttttattc caccattttt taaataaata
     4381 attctattgc atgttttata aagaaattcc ggatcatgag tggctataac tactgcaccc
     4441 ttttctttta agtctttaat aaggtcagca atcctttcca tattaatata atccaaccca
     4501 cttgtaggtt catcaaatag aaataaatta gtttttgaca atgaagctat agaaaccaat
     4561 aatctttgtt tttctccacc ggataaatca aacggtaatt gatctttcat tttataaagt
     4621 tccatttttt ttaaaatttt ttcttgctga tctttactaa ctatttcatt ccccagggct
     4681 aactcgctcc tcactgattc tgtgaaaaat tggtaatcca tttcttgcat tactaaaaac
     4741 ggcgatgaca aatttttttt attttcgatt atctttcctt tggtagggcg aataatcttc
     4801 atgagtattc ttagtaaggt tgttttcccc acaccgtttt tccctaccaa accaataaca
     4861 tctccaactt tgacatcaaa atttatgtct tttagaatat tttcataagt aatttcttca
     4921 acttttaata catctgcact taccttttcg atatagggaa cttcaatcga actattaaaa
     4981 atgtcaaatt tccttaatgc gttattgttt tcagttttta acaactgaat agttgtagtt
     5041 tttacagttc tatttttaac aaatagaact tgatcaatta attctttcaa atagtaaaaa
     5101 cgatgttcag ctataacaat agtaaatcct ttatttttta aatcacaaag taagtcagca
     5161 agtttcttag tcgctttgaa gtccaaattt gaagacggtt catccaatat aattaattta
     5221 gagtttagcg ataaactaca tccaatagaa attttctgcc gttcaccact tgatagcgaa
     5281 aaaatgtttt tatctaatat atttgaaaga tctaataatt cagctaattc attgattctt
     5341 ttatccatat gctcttttgt aaatccaaag ttttccatcg aaaatactaa ctcactcgtt
     5401 gtatttgttg taaagaattg tcctctagga tcttgactta cgtagccaat attttttgat
     5461 atactttctt gtgttaagga ttgtagcgag ttattttcaa ataaaatttc cccaaccaat
     5521 tttccgtcat aaagtcttgg aataagtcca ttcattaact ttaatagtgt ggatttccca
     5581 ctcccagaag aaccagtaat caatgtaaga ctaccttttt ttattgaaaa attaaggtca
     5641 acaagcgctt gttccttaac atccttatat gaaaaagaaa cattgttaaa ttcaaacaat
     5701 tctttcacct caaatcaaaa tacttaagaa aaaaatagta acggaaaaca ggatgatata
     5761 gtctaataag ctcatgtttg ttttatagta agaacttctt ttcacaggag attctatccc
     5821 ctttgttatt gcattagcgg tcatttcttc agcaatcata gttgcacgta taagaacggg
     5881 aactaacaga aattccaaat attgcagagg ctgaaaaaaa gacaagcgaa tgtttctcat
     5941 ttttaaagat tctttaatca ttcttatttc agaacgataa gtcggaaaga atctgatcac
     6001 tactgtaata cttaacatta tttttttcgg taaatgtatt ttttctaaag aggataataa
     6061 ttcattgcta gtatagacac taaccaacaa ccatgagatc attacgaccg gcaacattcg
     6121 aaatacaata tataaggatg tcccaaaaaa tatagtaaaa aactctatct tgatagcatc
     6181 aataattttc attactaaaa acagaactga ataaatcaag atgaaataaa tacatgtctt
     6241 tattttacga aacatcaata ctaaaactgt agcaataatg aaacacccca cttgaaatat
     6301 atttcccttt cctaaaaaaa taatgaccgg aaaaaaaata tttaaagtaa tcaaggttaa
     6361 cggattgatt tttgacactc tttcacctcc aaaaaataag atcgcactgt tatcttaagc
     6421 tgataaaaat gcaccgttat tcagataaca gtgcgatctt atcaaaaagc ataactgaga
     6481 acaattctca atgcaagctt ttttttaatt aactcagtaa ttcaatccac ccaggaacag
     6541 aaaattgata caatcttctg gttaatagca tagtcttttc ttttgaatag ctatgcttca
     6601 atatatcata gaatgtagcg tagaatgagt aaatgtagaa gtggatttct tcttcattaa
     6661 agactaaatt ttgattgatc attccgatag tctttaactt atctagacaa cagactgtct
     6721 tttttgtcac aaatgatgta attttttcaa caatatcttg atatttggtt ccagctgatt
     6781 ggaataaaac caaatcgaat atacgtttat tctcaaaaat ataattaata aatggatcta
     6841 attcattgaa gttctctctc caacttttct ctatatctat attttctaag tttaagaggt
     6901 atttggtaaa ctcttcatta tacatattaa taagcgcatt aatatgcggc tgaataattt
     6961 cttcaaataa cgcttcctta gaagtaaaat gtttataaaa agcacccgta gttacgcctg
     7021 cggtcaagca cagttgcctt atcttagttt gctgaaatcc tactttttca aaatggctta
     7081 gtccactttc tattaatttt tggtgagttg taatataatc tctggtttct atgggtactt
     7141 cctcctaata agatgaatta actatattat cttacacagg acttgaagtt tcaatcgtta
     7201 acgtctatta ctgaactcat tccttagagc taaactaatg agaaagagca aaataactga
     7261 tacaaaaagc aatcctacaa gaattaatag actcatctaa atcattcacc ttttctaaaa
     7321 taatccgaga aagatttaaa aaaattatat ccagctatta ggataagtcc tatcataatc
     7381 actccaatag ccattttctt atcctcccta aaaataaagc cttttactta aaaccttttt
     7441 tgtaatcgtc aataaatttt ttccctttat cagttaattg aaatcctata tctaaatagt
     7501 cctcatatct aagtttagtc agcatctcac ttacagatgc tggactaaca cttagaactt
     7561 ttgctaaatc attatttttt aatttctttt ttctttttga taattcctga attgctagta
     7621 aataaattag tatagtagtt gttttaattt gtaccatacc taccctccaa attatttcga
     7681 aaggccttca gcaattttat ccaggttcca tttcatcatc gcatagtaac tatcaccatc
     7741 ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa tagataggaa tattggtatc
     7801 ttttgatact gttttcattg gtctatcgtc cacactgctt tctacgaata aggagggaac
     7861 ttttgttgtg cgtaattttt caactaagtg ttttatttga tctggtgttc cttcttcttc
     7921 agtgttgatt tcccaaatgt aagcagaagg cacattatac gctttcgaga aatatttaaa
     7981 gcatccttca cttgtaacaa tcattttttt atcattcgga attgaagcaa atttagattt
     8041 agcttctttg tctagagaat ccaatttttc aatatactta tctagatttt ctttatagaa
     8101 ttttttatta tcaggatctt tttccgctaa ctgtttttca atatttttag cgtaaataat
     8161 accgttttct aaatttaacc aagcatgggg atcttccttc cctttctcac tctgaccctc
     8221 taagtaaata acatctatgc catcacttgc tgcaaaatag tctttgtttt cctctttgtt
     8281 cgcatttttt actaattttg taaaccaagc atttcctcca gtttccaagt taacaccgtt
     8341 ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct tcaggcaaag gttcatattc
     8401 gtggggatct ttcccaatag gtacgatact gtgtaaatca attttatctt ttgctatatt
     8461 ttcagtaata tctgctaaaa tcgaattagt aactactacc tttaattttt cgttcgaagc
     8521 cactgtatct ttgtctttac tatttgttga acatgccccg aatataaaaa gagcaatcaa
     8581 aatccccaga cttcccagta ctgctttcca ttttctcatc ataactttct ctccttcttt
     8641 cttaaaaatt tttgttttgg tgagattaga aaactaatta caaataagaa tgccgctgtt
     8701 aaaacaatac ttgatcctgc tgctatgtta aaactataac caatgaacaa acctagcaat
     8761 gatgagcacg ctccgactgt cgccgaaata aatatcatag tttttaaact gtttgcatat
     8821 aaatatgctg tagcagctgg tgtaattaac attgcaacaa ttaatatagt tcccacactt
     8881 tgcatagctg ttactgctac taaagttaat agaatcatta atagataatg ataaaaactg
     8941 acattcatcc ccattgcttt tgccattaat ggatcaaaag aagtgattaa taattcttta
     9001 aaaaatagaa caactgtaac caacactaaa a
//
LOCUS       DACESX010000106         9021 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.106, whole genome shotgun sequence.
ACCESSION   DACESX010000106 DACESX010000000
VERSION     DACESX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.106"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            125..349
                     /gene="nrdH"
                     /locus_tag="IXF88_002117"
     CDS             125..349
                     /gene="nrdH"
                     /locus_tag="IXF88_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1508835.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            508..2667
                     /gene="nrdE"
                     /locus_tag="IXF88_002118"
     CDS             508..2667
                     /gene="nrdE"
                     /locus_tag="IXF88_002118"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1508836.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2717..3682
                     /gene="nrdF"
                     /locus_tag="IXF88_002119"
     CDS             2717..3682
                     /gene="nrdF"
                     /locus_tag="IXF88_002119"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1508837.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3771..4910)
                     /locus_tag="IXF88_002120"
     CDS             complement(3771..4910)
                     /locus_tag="IXF88_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1508838.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5219..5932)
                     /locus_tag="IXF88_002121"
     CDS             complement(5219..5932)
                     /locus_tag="IXF88_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1508839.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6186..6887)
                     /gene="nagB"
                     /locus_tag="IXF88_002122"
     CDS             complement(6186..6887)
                     /gene="nagB"
                     /locus_tag="IXF88_002122"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1508840.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7121..8653
                     /locus_tag="IXF88_002123"
     CDS             7121..8653
                     /locus_tag="IXF88_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1508841.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8844..>9021)
                     /locus_tag="IXF88_002124"
     CDS             complement(8844..>9021)
                     /locus_tag="IXF88_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1508842.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         3010 a         1757 c         1586 g         2668 t
ORIGIN      
        1 cacaaaactt agtattccac agtttccaca acacaatatg tagtgtttgt tgtttgactt
       61 ttttactaag agttttatac tatctattat agataataag aatgtgttag aggaggcact
      121 caccatgaac gtaaaaatct tttctaaaaa taattgtatg caatgtaaga tggtcaaacg
      181 gtttttaaca gaaaatcaaa ttgcttttga agaagtcaat atcgatgaac agcctgaagc
      241 agtagactgg ttgaaagcac aaggattcca aagtgttcct gtcattactt ctgatgcaga
      301 aacagttatc ggattccgtc ctgatcagtt gcgtaaactt gccagctaac atgactaaaa
      361 gaatcaaaac gtgatacatg gatttgaagg gcttgagaca gcaccagttt ttgtcgcaaa
      421 cttccaaatc cttttttaaa gcttaaaaac aatgcaatga tttgcaatca agcatcatca
      481 aatacgaaat gaaggttggg cgtacgtatg agtcttaaaa atttacaaga tgtcagttat
      541 ttcaagctga ataatgaaat caatcggcca gtcaatggtc aaatcccttt gaataaggat
      601 aaagaagctt tagttgcctt ttttgaggaa aatgtcaaac caaatacgat gacttttcca
      661 tctattatgg aaaaaatcaa ttacttgatc gatcaagaat atatagaagc agaattcatt
      721 caattgtatt caccatcatt tattgaaaag ctatatcaat ttttggacga gcaacatttt
      781 tctttcaaat cttttatggc tgcttacaaa ttttattctc aatatgcgtt gaaaaccaac
      841 gacggttccg cttatctgga aacttatgaa gatcgagtag ccttcaatgc gctttacttt
      901 gcgaacgggg atgaagaatt agcacttgct cttgcagacg aaatgatcca tcaacgatat
      961 cagccagcta caccttcttt cttgaatgct ggacgtaaac gtcggggcga attggtgtct
     1021 tgtttcttga ctcaagtcac tgacgatatg aactctatcg ggcgctcaat caactctgct
     1081 ttgcaattgt cccgtattgg tggcggcgta gggattactt tatcaaatct tcgtgaagcc
     1141 ggcgctccta tcaaaggata tgagggtgct gcaagtggtg tcgttccagt catgaaatta
     1201 ttcgaagata gtttcagcta ttctaatcaa ttaggtcagc gtcaaggagc tggcgtggtt
     1261 tatttgaacg ttttccatcc agacattatt gctttccttt cagcaaaaaa agaaaatgca
     1321 gatgaaaaga ttcgcgtaaa aactctttct cttggcgtga tcgtacctga taaattttat
     1381 gaattgacac gtaaaaacga agatatgtat ttatttagtc catatagtgt cgaaaaagaa
     1441 tatggtgtcc cattctctta tgtaaatatt acagctgagt atgatcatct agttgcaaat
     1501 ccaaacattc gcaaacaaaa aatcaaagcg cgtgatcttg aaaatgaaat ctcaaaatta
     1561 caacaagaat ctggctatcc ttacgtaatc aatattgata cagcgaaccg cgagaatcca
     1621 atcgatggca agatcatcat gagtaattta tgttcagaga ttttacaagt tcaaacgcct
     1681 tctgtgatta acggcaaaca ggaatatgaa gtattaggaa cagatatctc atgtaactta
     1741 ggctcgacaa atatcgtgaa tttgatgcaa agccctaact ttggtcaatc tgtgcgtacg
     1801 atgactcgtg cattgacatt tattacagat gcatctgata tcgatgtcgt accaccgatc
     1861 caaaacggaa ataagctgaa tcatacgatt ggattaggtg ccatgggctt acacacttat
     1921 cttgcaaaag aacaaatgga atatggctca gaggattcat tagactttac aaatatttac
     1981 ttcatgctgt tgaattattg gacacttgtt gaaagcaaca atatcgctcg tgaaagaaaa
     2041 caagttttcc ataattttga aaaatcagct tatgctgacg gcacttattt cgaaaaatac
     2101 gtaactggag aattccagcc aaaatcagaa aaagtcaaag cattgtttga aggcattttt
     2161 atcccaactg ctgaagattg gaatgctttg aaacaagcag taatgaaaga tggactgtat
     2221 catcaaaatc gcttggcagt agcaccaaat ggctcaattt catatatcaa cgatacgagt
     2281 gcaagtattc atcctatcac tcgcatgatc gaagaacgcc aagagaagaa aatcggtaaa
     2341 atttattacc cagcgcctta tctatccaat gagacgattc cgtattacac ttctgcttat
     2401 gatatggaca tgcgtaaagt aatcgatgtt tatgcaacgg cacaacaaca cgtcgatcaa
     2461 ggcatgagtt tgactttatt catgcgttct gaaatcccag aaggcattta tgaatggaag
     2521 acaacaacga agcaaacgac acgtgatttg aatattttaa gacattacgc tttccacaaa
     2581 ggaatcaaat ccatttatta cgtacgtacc tttactgatg atgcagaaga aatcggaagc
     2641 aatcaatgtg aaagctgcgt gatctaatat catagataga ttcgcttatt catattttaa
     2701 ttaaaggagc attctcatgt cagaaacata ttatgaagca ataaactgga atgaaattga
     2761 agatatcatt gataaatcca cttgggagaa gctgacagag caattttggc tagatacacg
     2821 tatcccactt tcaaatgacc tagatgattg gcgtacattg tctcaattgg aaaaagatac
     2881 agtaggacat gtatttggcg gtttgacact gctagatact gtccaatctg aaagtggcat
     2941 ggatcagttg cgtaaagatg tccgcactcc ccatgaagaa gccgttttga acaatattca
     3001 attcatggaa tccgttcatg ccaaaagtta ttcttctatt tttagtacac tgaatacgaa
     3061 aaaagaaatc gaagaaattt ttgactggac aaatacgaat ccttatctgc aaaaaaaagc
     3121 tgagcgtatc aatgaaatct acaaacacgg tacaccgcta gaaaagaaaa ttgccagcgt
     3181 ctttttagaa acattcttgt tctattcagg tttttatact cctctttatt atttaggaaa
     3241 caacaaattg gctaacgtag cagagatcat caaactaatc atccgagatg aatctgtaca
     3301 tggaacctac atcggttaca aattccaatt aggattcaac gagttacctg aagcagaaca
     3361 agaaacgtta aaagactgga tgtacaatct tctttatgag ctttacgaaa acgaagaacg
     3421 ttatacagaa gaattatacg atccaatcgg ctggacagaa gaagtcaaga cattcctacg
     3481 ctataatgcc aacaaagcat tgatgaacct agggatggat ccactctttc cagatactgc
     3541 caatgatgtc aacccaatcg taatgaacgg gatttcaaca ggcacaagca accatgactt
     3601 cttctcacaa gtcggaaacg gctatttgtt aggtcatgta gaagcgatga aagacgacga
     3661 ttatttgatt ggattggaat aaggatttat tttaattcaa aaatttttcg aaaaataaaa
     3721 agagtttagc gtacagatat tttaaatgta cactgaactc ttttttatgc ttattcactt
     3781 gtttcttcca gcacttttgg actgtctaac ttttccgaaa catgttccat tcttctcaat
     3841 aaatagattt tccggatatt taatattatc gtcttaatca ctgcatagac aggtatgcaa
     3901 atcagcatgc ctaatattcc tgcgacactt cctgctccaa tcaataccaa gatgatcgtc
     3961 aaaggatgga tattcattga tttcccaaat aacaacggct tgatcaaatt tccatctaat
     4021 tgttggatca ctaaaatcga caacgccata tacaaagctt gaaatggaga gataaacaaa
     4081 ccgacaatga ttgctggtac cgctccaata aaagggccga catacgggat aatattcgtg
     4141 attccgcaaa tgaccgcaag caacaagcta taaggctgat cgaatattct cattgccaaa
     4201 aagctcatta caccaataat caaagcatct aagatcgtac tgctgatata ggcagataat
     4261 gtttcgttta attcttttac cgtttggcgt aactctttac gaatcgcttt cggaaagaag
     4321 tgagacaacg catcaataaa tttgtgccca tccttgaaca taaatagcaa aataaatgga
     4381 acggtaaata acaaaataaa aaattgcatc ataaatgaaa tgatttttga gacacttgca
     4441 gataaactga ctaatatgat attgaacaaa ttactaagag ataaatttgc tgatgctaat
     4501 tgttcttcca aattgaaatt tttaaactct tcccgctgcg ccaattcatt taaccatttg
     4561 cttgtttcat ctgcatacat tggcaagctc tgggtcaatt gaattgtctg ttccattaac
     4621 tgaggcacta cattcatgac agttaaaaca actaaaatga ttacgaccaa aaacgtcaat
     4681 aaaaaaccga acactctcgg gatccgatgc ttttcgagaa aaacaacgat tggatcgaac
     4741 atataataga taaaaccagc aacaagcaat ggtaaaaaaa ttgccgaaag cattttcaat
     4801 attggactaa aaatattcgg catttgtaaa ataaagtaca cgccgatgat cgtgagtact
     4861 agctccactg tccagaaaaa taattttgat tgtttaaaac gtgattgcat acatctcctc
     4921 caaggtcctt tgatacttct attctatcat ttaacaaaga gttacgcgct gagattttca
     4981 tttgcttaaa aaaaacaaaa aaaataaaca tattttaatg agtagacgag tacttaaaca
     5041 aaaacacggt ttggagtagt gacaaaaatt atactgcagt ccctttttcc aaataaacgg
     5101 tgttcaaaag cttgcatctt cgtaataagc ccagccaaac gaaaaatata aggaactatt
     5161 tttatttggc tgggcttatt acttgatgta ggacatctat tttacaacct cagatcattt
     5221 attgcgcaat actgactaaa tgcaactttg tgatattttt gtgtacggac aactcctcga
     5281 tcacatcggc ataagtgagc ccttttggaa gatcaatcgt atagatattt ttgtacactc
     5341 ggtagtcatc aacgaatttt acgtcaaaat tcacatcttc gattacgata tttttctctt
     5401 caaaataatc ggtaagaaaa tctttggttt cttttctatg gataaattgg atttctaact
     5461 ttttagtcgt aggtacatgg atcacgcgtt tcactactgt gagtgcaata gcaatcccga
     5521 taaaactgat gatcgcaatg gaatagtaac ccatgccgat ggcgatccct aatccagcaa
     5581 ttgcccataa agaagcggca gtcgtaagtc ccgtcaccga ttgtttcgtg acgatgatcg
     5641 ttcccgcgcc taagaaaccg acaccactca ccacttgagc aatcagcctt gcttcatctg
     5701 aacgaatcgt tccagcaagc tgtggatact ctaaagcatc tcttaatgca ccactagcga
     5761 tctcaacttg gatcaatgca atgatacatg ctcccataca aactaaaata tgcgtacgca
     5821 tacctgctgg acgattttta tattgtcgtt caaaaccgat tgctccgcca aatagcatag
     5881 cgatccctaa acgtaaaata atctctggaa ttgtcaatac catatcattc atctaaacac
     5941 cctccagtcc aatatatcat gtctgaaagg agcagacaaa aaaagtgcaa tttttacctc
     6001 ttacgaaaaa aggactgtga caaaattttt gtcatagtcc tttttatgaa taaacggtgt
     6061 tcaatcagct gaccctcggt attactcaac aaacggcaaa atatggggag ctatttttcc
     6121 tttgtcttct ctaatactca ggtcaaaacg ctgatttcac aacctcttta gcctactatt
     6181 gaaaattaca acttacttgc tgcttcttca tcgatgatca caacaacatt gtcgtgattt
     6241 tgcaatacac ttgctggcaa atgattcgtt acaggtcctt caatcatccc tttgattgca
     6301 tcagctttgc tttcgccaaa tgccatcaag acgatttctt tacttttcat aattgaaccg
     6361 attcccattg aaacagcttt tgttggtaca tcttctgctt tatcgaagaa tcgtttgttt
     6421 gcattgattg ttgattctgt taagtcaact actgaagtcg ttccgtcaaa tgaagcacca
     6481 ggttcattga aaccgatatg tccattttgc ccgattccta agatttggat atcgattgga
     6541 tgtgcatcga tgatttgatc atactttttg catgctgctt ccagatcttc tgcttttcca
     6601 tctggtacaa acgtttcttt gaatggtttt ttatcaaata attggacatc catgaaatga
     6661 cggtagcttt gttcatctga accgcctagt ccaacgtact catctaagtt tacagaagtc
     6721 atttcactga aatccaaatc gcttgaagtc atttctttat ataaagtaac tggtgtgctt
     6781 cctgttgcca atcctaatac ttttgcacct ttgttcatac cttctttgat gatttcaaat
     6841 gctttttttc cgccttcttc agcgtttttt acacgaatga tttccatagt gattgctcct
     6901 ttatattttg gtatagccca attatagcat tctttttttc tttttacaat aattagtata
     6961 gaccaaattg catttttttc attttcttga caataaatag gaatccgtgt taggtttcat
     7021 aagatggttc aatcaaacaa tccatgttgc atgaaaacca agcaagtgat tcactgtgga
     7081 cgctttatgt ctgcagtttt tttatgaaaa aggagttatt atgagtatac tgacattaaa
     7141 caacattacc cagcaatttg gcgacaaaat cctctatgaa ggtgtcgatc tgcaattaaa
     7201 tgctggcgaa catcttgggc tgattggaca aaacggagcc ggtaaaagta cattgattaa
     7261 aatcatcact ggtgagatct taccagatga cggacagatc cattggcaaa aaaatattca
     7321 caaaggttac ctcgatcaat atgtggaagt cgatgaaaca ttaacaatcg aagaatttct
     7381 aaaaactgcg tacgcaaaag aatacgaaaa ggaagaaaaa attgcttttc tttaccaaac
     7441 atatagcgag accttcgatg attccttgct ggaaaaagct gggaaactcc aaacggaatt
     7501 agaccaagga gtgttttatc aaatcgacac cttagttgct gagatgagca gcgggttggg
     7561 gatcgatgtc ttaggtttag attcactgtt gaaaaatcta agtggtggac aacgttcaaa
     7621 ggtgatttta gcaaaactac ttttagaaaa cccagatgtt ctgatcttag atgaaccaac
     7681 caatcactta gatgatcagc atatccaatg gctgacgcag tttcttcagg attttgacgg
     7741 tgcgtatatc gtgatttccc atgaccaaga atttcttgat cagatcacca cccatatcgc
     7801 agacatcgag tttggaaaga ttactaagta tactggccat ctaaaacaag cattgaaaca
     7861 aaaggaacaa aatcgcgaaa gctatttgcg ccaatatcat gctcaacaaa aacaaatcga
     7921 aaagaccgaa gcttatattc gaaaatacaa agccggttcc cgatcaacga tggcaaaaag
     7981 ccgacaaaaa caattagata aaatcgaacg gctgacgcct ccagcttcag caagtaaagc
     8041 tgtctttgat tttccattct cgcctattgt cacgacatta gcagtggaaa caactgattt
     8101 agtcatcgga tatgatcgac cgctacttgc acccatcaat ttgaccatcc gctttggtga
     8161 aaatattgcc attcgaggct tcaacgggat tggaaaatct actttattaa aaacactgat
     8221 cggtgagatt gaaaaaataa gtggcgattt ccacttccca gataacacaa aaatcaatta
     8281 cttttcacaa gatctgtact gggaaaatcc attagaaaca ccactacaat atttaagcag
     8341 tcaatttcca aaagcgacta tcaaagaatt acgtcgccaa ctagctaaag cagggttagt
     8401 taatcaatta gcaagtgaac ctctcactac attaagtggt ggggaacaaa ctaaggtgaa
     8461 acttgcacag ttgacaatga accaaggtaa tttactgatt ctcgatgaac cgacgaacca
     8521 tatcgatcaa gaaacgaaag aaagcttaca agacgggata cagaaatttc caggaacagt
     8581 aatcatcgtc tctcatgaac aagaatttta tcaagatctt gtgcaacgag tcattgagat
     8641 tgaaccaaaa taaacgtttg attgaatagt tgaaaaaaag gagctgtgac aaaagttttg
     8701 tcacaactcc ttcttctgaa taaatggtgg ccaattagct gaccttcggt attagctcaa
     8761 aaaacggcaa aatatgagga gctattttta tttggctatt cttattactt gatgcagaac
     8821 agcttttcac aacctctttt cgtctatttt ttattttcga gatttctctt tcctttaaaa
     8881 acaactgttt gaaagaagaa cgcacatcct tgaaaaacaa gcccgctaac cgttacacct
     8941 aaccagccat actgctgcca cattgtcgtc cctagcaatg aaccagccgc acctccaata
     9001 aagtacataa acataaatac a
//
LOCUS       DACESX010000107         8816 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.107, whole genome shotgun sequence.
ACCESSION   DACESX010000107 DACESX010000000
VERSION     DACESX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.107"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(137..427)
                     /locus_tag="IXF88_002125"
     CDS             complement(137..427)
                     /locus_tag="IXF88_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508843.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="IXF88_002126"
     CDS             complement(545..805)
                     /locus_tag="IXF88_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508844.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="IXF88_002127"
     CDS             complement(942..1355)
                     /locus_tag="IXF88_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ1508845.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="IXF88_002128"
     CDS             complement(1811..2296)
                     /locus_tag="IXF88_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508846.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="IXF88_002129"
     CDS             complement(2435..2650)
                     /locus_tag="IXF88_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1508847.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="IXF88_002130"
     CDS             2874..3674
                     /locus_tag="IXF88_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1508848.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5560
                     /locus_tag="IXF88_002131"
                     /pseudo
     CDS             3693..5560
                     /locus_tag="IXF88_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            5553..6044
                     /locus_tag="IXF88_002132"
     CDS             5553..6044
                     /locus_tag="IXF88_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1508849.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="IXF88_002133"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="IXF88_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1508850.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..7599
                     /locus_tag="IXF88_002134"
     CDS             6604..7599
                     /locus_tag="IXF88_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1508851.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(7741..>8816)
                     /locus_tag="IXF88_002135"
     CDS             complement(7741..>8816)
                     /locus_tag="IXF88_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1508852.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         3045 a         1425 c         1683 g         2663 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctgag tttagtctaa tggtgataag
     7621 tcaagatgaa atttaagatt ttctttattg agtaatatgt tttgaagcgc acgaaactag
     7681 acccgatcaa aatcaaattt ttttcgggtt gattcaagga tttgcgtcaa taacggattg
     7741 ttatttctct atcacactcc ttggtggcgt atagagttgg tgtttacgta gtagcgtatc
     7801 caccagacgc gtaaattttc ttgcggttag gacgagtgca cgtttgtgtt gatgctttgg
     7861 aacctcctta tatttacttt gataaaaagc tttgtattca ggaagataat tttttacaga
     7921 gttggcagct tcaactaagt agtatctaaa atatcgattt ccttgttttg tcagaggtgt
     7981 attttgcgac tgatagtttc cagattggtt cactttccaa cttaatccag cgtattttgc
     8041 caattttgtc tgatcttcaa atctttcgat ttgaccaatt tcagcgatta aaccggcagc
     8101 atagacttta ccaacgcctg gaatactggt taagcattga tattctggaa tgacaacaat
     8161 tacttgttcg atggctttat ctaattcttt gatatttttt tcaagtgctc gtatttctcg
     8221 tacgagaaca cttaaaacaa cattaattga ttcttgtgca agagcaccta ggcggtaact
     8281 catggtaatt gcacgttgaa ttgctttagc gattttttct ggttgcttga aacggccttt
     8341 tcctttttct tgaagtaaat cacagaaagc ttctaaagga agctcagcaa gttcatccaa
     8401 tgtgaaatct tccgtcatga gtgaaatgag ggttgcacta aaaagactcg tcgattcgtc
     8461 tcgcatttct cgagcaagcg tattacattt gtacgttaga ttttcaagaa aatgttgctt
     8521 tgtacgaatc aattgcttaa taagttgata gcgagtacgt gttaaacgct gtaaggccat
     8581 gtatttctct tctttgatag gggaagttgt gaatcgttga atacgcaaga aatcagcgat
     8641 tcttaatgca tcgtttcgat cagttttatt ctcatcaaac atgcgactaa attgtttcac
     8701 ccgaaaagga ttttcaatcg ttacaagtgt gttgagcttc tttagtttct catcttcatg
     8761 aaaaaacatg gaaggatgga agctgtacat ggacgttgac tccatgccaa tcacaa
//
LOCUS       DACESX010000108         8672 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.108, whole genome shotgun sequence.
ACCESSION   DACESX010000108 DACESX010000000
VERSION     DACESX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.108"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(93..539)
                     /locus_tag="IXF88_002136"
     CDS             complement(93..539)
                     /locus_tag="IXF88_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1508853.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(566..742)
                     /locus_tag="IXF88_002137"
     CDS             complement(566..742)
                     /locus_tag="IXF88_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1508854.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(904..1332)
                     /locus_tag="IXF88_002138"
     CDS             complement(904..1332)
                     /locus_tag="IXF88_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1508855.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1424..2284
                     /locus_tag="IXF88_002139"
     CDS             1424..2284
                     /locus_tag="IXF88_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1508856.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(2376..2669)
                     /locus_tag="IXF88_002140"
     CDS             complement(2376..2669)
                     /locus_tag="IXF88_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1508857.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(2741..4468)
                     /locus_tag="IXF88_002141"
     CDS             complement(2741..4468)
                     /locus_tag="IXF88_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1508858.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(4682..5608)
                     /gene="trxB"
                     /locus_tag="IXF88_002142"
     CDS             complement(4682..5608)
                     /gene="trxB"
                     /locus_tag="IXF88_002142"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1508859.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(5753..7186)
                     /gene="ascB"
                     /locus_tag="IXF88_002143"
     CDS             complement(5753..7186)
                     /gene="ascB"
                     /locus_tag="IXF88_002143"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1508860.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(7186..8589)
                     /locus_tag="IXF88_002144"
     CDS             complement(7186..8589)
                     /locus_tag="IXF88_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1508861.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         2357 a         1943 c         1468 g         2904 t
ORIGIN      
        1 aataagattc tttcttctag ctgtttttta gttctttttt tacgaacaca aaaaactgcg
       61 gataatcact gtccgcagtt taaatgataa aattaagatt gtaatagttc tttcacaatc
      121 gcattcactt ggtttccatc tgcttttcct ttgactttag gcataattgc acccataact
      181 ttgccaaatt ctttcattga tgaagcacca gtttgagtga ttgcttcttg aacaagttgt
      241 cgaatttctt cgtcggaaag ttgttccggc atatattttt cgactattac aatttcactt
      301 tttacttttt cagctaaatc gtcacgtcct gcttcttcaa actcatgcaa agaatcacgt
      361 cgctgtttca tttcacgaga caacaccgtt agttcttctt cttctgtcaa gtcgtgacct
      421 tctttaatct gctcgttttg tatagatgac ttgatcattc ggatgacttg taaagattcc
      481 ttatctttcg ctttcatcgc tgctttcatg tcatcgttta atctacttag tagtgacaca
      541 ctaccaactc caattcatca cagaactaga attttttgcg ttttctagct gcttcagatt
      601 ttttcttgcg tcttacactt ggtttttcgt agaattcacg tttacgagat tcttgtaaag
      661 tacccgcttt tgacacggaa cgtttgaagc gacgaagagc atcgtcaagt gattcgtttt
      721 tgcgaacgac tgtttttgac atgttaattc cctccctccg agcttaaaaa gtaaccgtaa
      781 ttgttattga aatatatctc aaaaaacaag actgtcctgt aaatattata tctaatgctg
      841 ttttgaccgt caaggaaatc ttgctttttt ttaaaattat caaatgttat tttatttaaa
      901 tccttattga caattttcac atcttccgaa aatctcgaat ctatgccctt cgatcgtgca
      961 tccacctagc tgttcttcga aataattcat cggacacata tggatttctt tcgttgttcc
     1021 gcacactgta cagataaaat gatggtgatg gccgattttc ccctgacagc aatgaaaacg
     1081 aaacttcatt tctccattta aatcggtttc ttccagtaaa tcgatctctg caaaatcatg
     1141 gagattccgg taaatcgtat cataacttac ccctttgaat tcctgattca tatagtcaaa
     1201 aacttcttta gcagatacgt agcggttacg cttcactaaa taagttaaca atgcttctct
     1261 ttttttagta tatttaaaac cgttattttt caatgtttct aatgattgtt cgatcaatgt
     1321 ctgttttttc atgacttatc cctccaaatc aaaatgatta cgaagtacgc attctagagt
     1381 atggtataat aaacataaaa aaaatgcaag tgaggtttta tccatgactt ccgattctac
     1441 agaaatcgtt actttttttg tcatctccga ctctgctgga gaaactgcga caaaactagc
     1501 gcaagctact atggctcagt atcctaccgt tgagttcaac ctattccgca gaacgttcgt
     1561 gacagatgaa aaaacattaa ggcaggcatt gcaagatgca ttagaagaaa aagcacttgt
     1621 cctgcataca ttaatcaatc aagaactgat cgagataaca cgttcttttt gtgaagaaca
     1681 tcaattattt agttttgatg tgatgacgcc accgatatca gaaattgaac gtttaactgg
     1741 tatcaagcca attcggcaac ctggtgccct ccatttattg aatgagaact atttcaaacg
     1801 gatcaaagca atggaatttg ctgtaaaata cgatgatggg aaagatcctc gtggtttttt
     1861 agaagcagat gtcgttttgc taggcgtatc ccgaacttcc aaaacgcctt tgagcttgtt
     1921 tttagctaac aaaaatttaa aagttgccaa tttacctttg atcccacaag cacatattcc
     1981 caaacagctt tgggagatcg atcctaaaaa aatcgtggga ctaacaaata acccagatat
     2041 tttaaataat atccgtaaag aacggatgcg ctcttacgga ttaaaccctg atactgctta
     2101 ttccgatatc gaaaaaatta gagctgaact cgattttgcg aatgatcttt atgaaaaact
     2161 aggatgtgta gtgatcaatg tagcatcttt atctattgaa gaaacggctt cgcttatttt
     2221 gaatgcttta gacctagaag atcatagtta ttatggaact gaaacaacag aaaaaagcga
     2281 ttgatcattt ttgtcttttt cttaaaaaac actggagtgt gacaacgaaa tttcttcgtt
     2341 gtcacactcc agtgtttatc tatttgcacc tatcgttatt gatgttccac atgtttgagt
     2401 gtctggttca aaatatccag tacatgagaa tcatcaagac tatacaaaat cgcttttccc
     2461 tcgcgatgag acttgacgac atgattttct cttaataaac gtagttgatg cgatacagca
     2521 gactgctcca tttccactgc ttgggtgatt gctgtcacat tttgttcccc gtctttcaga
     2581 tatagcagaa ttttaagacg agtgggatca ctcaacattt tgaataattg actgactttc
     2641 tctacttttg atttttgcgt gtctggcaat ccattcacct ccaacttgaa aaaagactga
     2701 cttctaaagt ttaccacttt ttccatcagt ctcaagtttt ttattcttct gtcatctttt
     2761 tgataatcgc ttcataagag gctacttttt cggctgcgtc ttctgtagaa cttcctttta
     2821 caccaatata aaacttgatt ttcggctcag ttcctgaagg gcggatagca atccagccat
     2881 tttcttccaa tgtatacttc aatacgtcgg atggcggtgt tgttagtttt tctacgtttc
     2941 cttcacggtc tgttctcgtc aattctttaa agtcttctgt ttgaataact tttgctccgg
     3001 caaattctgt cggtgcttct ttacggaatt tttccatcaa agcagagatt ttcgcagaac
     3061 cttcttgtcc gctcattgtc acagaaatcg ttttttctgc gaaatagcca taggtttcaa
     3121 aaatcgtttg caagccgtca tacaacgttt tgccttgttt tttatagtaa gctgctactt
     3181 ctgctaataa aagcaatgct tggatcgcgt ctttatcgcg aacaaaaggt ttaactaaat
     3241 aaccataact ttcttcgaat ccaaacataa aagtatgtga atggtcttct tcaaattgtt
     3301 ggattttttc agcaatgaat ttaaaccctg tcaacacatt gaacatggta gcaccgtagt
     3361 gttccgcaat tgctgtaggc aattcgctgg aaacgattga tttcaatacc gctgcgtttt
     3421 ctggtaaagt gcctcttgat tgatgtgctt ccaaaatata ttgagcaaac aatgctccga
     3481 tttggttccc agtcaatact tcataagaac catctggcaa acgaaccgct gcgcctaaac
     3541 ggtctgcatc aggatctgtt gcaatcagca agtcagctcc ttctttttct ccaagtcgta
     3601 tagcatattc aaaagctgaa tgttcttctg gatttggcga tttgaccgta gtgaaatctg
     3661 gatctgccac agcttgttcg ggaactagta cgaattgttc gaatccagct tgtttcaacg
     3721 cacgttcgcc caacattttc cctgttccat gaagtggcgt gtacacaagt ttcaaatctt
     3781 tccccattgt ttcaaccaat tcacgatcaa tcgtcactgc tttgatttct tctagataag
     3841 cagcgtccac ttcttctccg atgatcgtga tcaacccgct atgtttagct tcttcgtcag
     3901 ataatacttc gacttttagc ggattgctga cttcacggac atatttcgtc aatgcatctg
     3961 cgtctgcagg aggcatttgt ccgccatctt ctccgtagac tttatatcca ttgtaagccg
     4021 ctgggttgtg agatgcggtt accatgattc ccgcaaacgt cttcaaatga cgaactgcaa
     4081 aagaaagctc tggtgttgga cgcagacttt cgaatacata agacgggata ttgtgatgtg
     4141 ctagtgtttt ggctgcttcc attgcaaatt ctggtgactg atggcgtgag tcataagcga
     4201 tagcgacacc tcggcgtttt gtttcttcac cttgtgtatc catgaaacgt gctagtcctt
     4261 ctgttgcttg tcttaccgta tagatgttca ttcgattgat cccaggtcct aagattcccc
     4321 gcatacctgc agtaccgaat tccaacggtg cataaaaagc atcttcaagt ttttcaggat
     4381 cttcacccag atcatttaat tgttttttta agttttcttc taaattttct tcatgtaccc
     4441 attgttcata cacttgttcc caagacatga aaataacacc tctttctgaa atatggttct
     4501 taataagtat agcattcact tattagatat gaaagtatga ggaccaaaaa acgcttaaat
     4561 caagaaagga ttcttttatc aaaaggtgtt ggacgtgttc gcataaatca gattcagtaa
     4621 atttcacgcg aaaaaaaaga gtaaaagaac aattgaaccc gttcttctac tctttttaaa
     4681 cttatgctgt tgtttcttct aaagactcca agtatttata cacttgttgt ccagcaatac
     4741 cgccgtcacc aacagctgta gtgatttgtc gcaaatcttt ttcacgaaca tctccgatgg
     4801 caaaaacgcc tggcatattc gttttcattt cgttgtctgt tggaatccag ccagcttcgt
     4861 tcgtcaaacc tgcttctcta aaaggtgcag ttaatggatc cagtccaaca tagatgaata
     4921 ctccgtctgc tgctaaatca aattcattgc cggttttgac attacgtgct ttcacacctg
     4981 tcactaccat ttcgtttcca acgatttcgt caacgactgt atcccacata aaggaaattt
     5041 tttcatttgc aaatgcacgg tcttggatga tcttttgtgc acgtaattca tctctacggt
     5101 ggacgatcac tacttcagaa gcaaattgtg tcaaatagat ggcttcttct actgctgagt
     5161 ccccaccccc gatgactact agacgtttgt tacggaaaaa cgcaccatca cagactgcac
     5221 agtaagaaac gcctcgtcca gcaaattctt cctcgccagg tacgcctaat ttacggtgga
     5281 tacatcctgt tgcgataatg actgtttttc cttcgtaact tttatcttca cagatgactt
     5341 ctttatggct gccgtgatcc ttgatatctt gaacgattcc ataagcattt tctgtcccaa
     5401 atcttgagac actttcatac atcttataag ctagatcggg gcccatgatc gaatcgaatc
     5461 caggataatt ttcgatttct gccgtattgt tcatttggcc gccaggggca ccttgttcga
     5521 tcatcaatac ggataagttt gaacgtgaag catataaagc agcagtcatc ccagcaggac
     5581 ctgcgccaat aacgattaca tcatacatat gtagattctc ctttacttaa tcaagtaagc
     5641 atactttaca gccaatgcga aaaaaagtct attgttctgc ttgcgtttct cgaatagctt
     5701 tattatactc gaaaaatccg ctgaaaaaca atagactttt ataaatcatt gatcaatcgt
     5761 tcgaaagatt ttctccattt gaatcaatta cttttttata ccagttgaat gattttttct
     5821 ttgaacgggc taatgtgcca tttccttggt tatcacgatc gacataaatg aatccgtacc
     5881 ttttcttcat ctcaccggta ccagctgata ctaaatcaat acatccccaa gtcgtatagc
     5941 ctaatagatc gactccgtct agttctactg catccttcat cgcctggata tgcgctgcca
     6001 ggtaatcgat tcggtaatca tcttccacat aaccattttc atctggcgta tcgactgcac
     6061 ctaagccgtt ttctacaata aacaatggtt tttgataacg atcataaata tcattcatcg
     6121 ttattctcaa gcctaatgga tcaatttgcc acccccattc acttgcttgc agatatggat
     6181 ttttgacaga agcaaagata tttcctgcag tctgttccaa gagttcgttg tcggtagtcg
     6241 aaactcggga agcataataa gaaaaagaga taaaatcaac agtatgttct ttcagcaatg
     6301 cggcatcccc tggttccatt tcgatctgaa tgccatttcg ctccatttct ttcaatgcat
     6361 aagcgggata ttctcctcgt gactggacat caatgaagaa ataattttct cgatctgatt
     6421 ttctagaagc ccaaacatct tcaggattgc aagtgtatgg ataatatgct cctgctgcca
     6481 gcatacagcc tactttgttc tccggatcta cttcatgagc aatcttcgtt gcgatggcac
     6541 ttgcaaccaa ctcatgatgt gccgcttgat atttgatttg ttcttcattt tctccttttt
     6601 caaaatacag ccctgcgccc atgaatggtg catgaaggat catattgatc tcattgaatg
     6661 tcagccaata tttgacaagt cctttatagc gattgaagat gactcgacac agattttcat
     6721 aaaaaccaac gagttctctg gaacgccacg caccatattc tttgatcaaa tgcattggac
     6781 aatcaaaatg cgtaatcgtc acaagtggct caattccgtg cttttggcat tctttaaagg
     6841 catcctcata aaatttcaag ccagcttcat tcggctcttt ctcatccccc attggaaaaa
     6901 tacgactcca ggcgatggac attcgataag tcttgaaccc catttctgca aacaaagcaa
     6961 tatcttcttt ataacgatga tacatatcga ttgcaatttt tgctggataa aaatgatcct
     7021 catcaaattc aaacattttt ttctttccgg tgataacagg aaaacggtct tttccgattg
     7081 gcacaacatc tacgtttgct agtccgcgtc caccttcatc ataaccgccc tcacattgat
     7141 tggctgctgt agcaccgccc cataaaaaat cttttctgaa tcccattata cttcctccat
     7201 tatatgtgta ttacctgata attctcttgc cagttcatct tgaacttcga ttccttcttc
     7261 ttttaatcgg ctgattgtct ccttgaattg cggtaaatac tgctggtgtg caataaacaa
     7321 ctcatccatg atccgttttg ctgtctttcc tgatcgtata agtggattga tcgtaaacgc
     7381 ctgtaaagct gtcccataat caccagtcac agctgcttcg atcgtcagca attccatatt
     7441 cttcattact tgcaaccagc ctctttcagc agcaggaaga gaaccaaacg ctacatttct
     7501 cgctccttgt gcgccaacat aggcagtgac ttctactaca caatctgccg gcaggtcagg
     7561 aacagctcca tcattctttg tagataccac gatctgtgtt tctttatttc catagattga
     7621 agcaatcgtt tcacaggcag cgtctgaata ataagcgcca cctcgttttt gcaattgttc
     7681 aggtttatgg tctaattctg gatctttgta taattcaaat agctctgctt ctgtttcttt
     7741 cacttgctgc gctcttgtac caatcgtttg atattcttct aaagcatgtt ccagcatttc
     7801 ttcttcacga tagtaatagc ggtggtagcc acaagggatc atgttcattt gttttaattg
     7861 ttctttgaaa aacgggatat caaagatatt tttcggtaat ccattctctt catcgtataa
     7921 acgatcaatc aattctgccg tgcggtcatt tcctttatta tctgctactt tgtgccaatg
     7981 gaaatgattt agaccggcaa acttgtagat cagctcttct tttttcttgc caagcattgc
     8041 tggttctgtc atcatcgcca tcactggcac attgcataat ccaatcactt tatcccattt
     8101 gccatatctg ataactgctt ctgtcaccat cccacttggg ttcgcgaaat tgatcagcca
     8161 agcatttgga cataaacgct tcatgtcctc aacgattgcc aaaacgactg gaacggtacg
     8221 aaaagctttg aatatcccgc cagcaccatt tgtttcttgc ccaatcatcc cataataact
     8281 agggatccgc tcgtctttga ttcgtgcatc taattgaccg acacgaaact gagtcgtaac
     8341 aaaatccgca tctttcaatg cttcttcccg atccagagtt gcatagatct tgacttcata
     8401 cggagaagca tcccacatac gctgagacat ctgactaacg atctcaagct tttcttttcc
     8461 ttcttctaca tctacgagcc agatttctcg tatcggtaat tcctcatatc ttttgataaa
     8521 tccttccatc aactcggggg tgtaactgct gcctccacca atcgtagcga ttttgattcc
     8581 ttttgccatg ttctcactcc tttacttttt tacactttca ttatcttttt tgtaaacaaa
     8641 acaaacaata gaaatctgtt tctttccagt aa
//
LOCUS       DACESX010000109         8581 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.109, whole genome shotgun sequence.
ACCESSION   DACESX010000109 DACESX010000000
VERSION     DACESX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.109"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(15..1058)
                     /locus_tag="IXF88_002145"
     CDS             complement(15..1058)
                     /locus_tag="IXF88_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1508862.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1435..2400)
                     /locus_tag="IXF88_002146"
     CDS             complement(1435..2400)
                     /locus_tag="IXF88_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1508863.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2634..3479
                     /locus_tag="IXF88_002147"
     CDS             2634..3479
                     /locus_tag="IXF88_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1508864.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3670..4857
                     /locus_tag="IXF88_002148"
     CDS             3670..4857
                     /locus_tag="IXF88_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1508865.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4863..5219
                     /locus_tag="IXF88_002149"
     CDS             4863..5219
                     /locus_tag="IXF88_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1508866.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5221..5559
                     /locus_tag="IXF88_002150"
     CDS             5221..5559
                     /locus_tag="IXF88_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1508867.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            5954..6988
                     /locus_tag="IXF88_002151"
     CDS             5954..6988
                     /locus_tag="IXF88_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1508868.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            6981..7667
                     /locus_tag="IXF88_002152"
     CDS             6981..7667
                     /locus_tag="IXF88_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508869.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7691..8539
                     /locus_tag="IXF88_002153"
     CDS             7691..8539
                     /locus_tag="IXF88_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1508870.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         2857 a         1563 c         1835 g         2326 t
ORIGIN      
        1 ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat cactgtttgg
       61 agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat gaattttgca
      121 aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct gctgataaaa
      181 tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa acaaaagatc
      241 acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt atcagttggc
      301 gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata gacaacaaca
      361 acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac agaattgtca
      421 agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc caaaagtgca
      481 cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag agtcaacatc
      541 atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc ctctttcgca
      601 tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt tcctccagcc
      661 atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta agcaccgatg
      721 atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat ttgagcaatc
      781 gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag tttgctttca
      841 gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc taataaaata
      901 cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc aagaagcctg
      961 ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg gaccgctgaa
     1021 tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc tttcgtcaat
     1081 aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac aaaagaacaa
     1141 gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt tccagaagta
     1201 gcttctacgg aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc
     1261 ttcttatttc ttgaagctga ccacttcttt tacgacctct tggtaaacgg tgttcaaaag
     1321 cttgcatctg cggtaataag cccaaccagt acaaaaacat gaacagctgt ttttgtttgg
     1381 ccgttcttat tacttgatgc agaacagctt tttcacaacc tattgaatcg attattaagc
     1441 gattttttta gaaacaaatt ttccatgttt gctatgttct ttaattaaga aacgtacgta
     1501 atgagcaaga acgtagaaga caactacact tgcaaacaga cattccgcaa cttttaagaa
     1561 atccaatacg ctactataga tacctaaact gttgaagtaa tgaacagtag taaatatagc
     1621 tgttgcagag ataacaagcg ggaatgtaaa ggctgcatag cttggataaa aacgaagctt
     1681 gaacaaacgt ggaagcatag acagcacaac gaaatacaat acttgagata aagcgaacaa
     1741 tgctacgaca agataaaaat taggttcagc aaagttttta agataacctg ttaggcaaag
     1801 agaaccaggt gctgcaacga tcgtaatcag cggacgtgtc ggatgagcca tatttcttac
     1861 gaaaaagaca cgtttgatga tgatcggcaa taagaacgca tagcagatca atgaaatcca
     1921 aaagaaaatt tttccaataa gtgggaagaa gttcccggca gttacagtga tgaccccaaa
     1981 tccaacgtaa atgataaacc agcttggata aattgcttca attgaaagat cagctttgat
     2041 aacatggtga taattgaaat aaaagacaag gatcacttgg aagataacag cgcacagcca
     2101 aatgaatttg acaattggtg caatactttc atatgaaaca aaataagtac aaataaccat
     2161 gagtgacatc gtaaaagttg gtgaaacgga ggcgatgatt ggatcatgca acgtttgttt
     2221 cgtgtgttcg aataagataa acaatttaac aaaaatgaat atcataagaa tcatagagat
     2281 accgccagtt agattgccta aagcaacatg gtgatagtct ttaaacagat ttccaaggga
     2341 agctaaccca aggatcaacc cgcagatcgg gatcggaata attttcaaaa agttcttcat
     2401 atataatatt ctccttttta tttaatagat tttagtacca agatgaacga atacattgta
     2461 aaatgaatag gaatgtatga ttacgagcaa gcagacagct gattgtcttc atctaagtta
     2521 ctagttggat cagcaactgt tttgttgtac atttctatta tagatggttt tttgtatatt
     2581 taaatcaaat agtttttata gtaattatca taaaaagtta tggaagtggt gccatgttta
     2641 agttattaac gacttttcga gcagtatatg aaacaagaaa tttttccaga gcagcagaag
     2701 tactgttttt atctcaacca gcagtctcga atcaaatcaa acagttggag cgggaattga
     2761 atatcgatct ttttcaacgt aacggaagaa aagagatgct tccaactaag caagccgact
     2821 tattatatga acggacactg gttttgttag aagaatggga agaaactcag caaaaattgc
     2881 tcaatgaaca aaatgaagaa gaagtatgtc ggatcgttgc ttcccacacc tttgctgtct
     2941 atcttttgcc tcagttgatg aaacatctga tcaaaaggtt tcctaaagtg cattttgaga
     3001 tcgaaatcac gaattctcat gaatcattag aacaagtagc gaaacatgaa tccgattttg
     3061 gttttatcga aaagccatta gagacttccg gcgtacaaag atactctttg atggaagacc
     3121 agctagtcat cgctggggac ccaaaaagca aattgtggct tgtaagagaa gcaaactctg
     3181 gtgtttttca ttacacgcag cgttattttg aagagatgaa tatccaagat aagaagatga
     3241 tcgtgaaaag taacgatgta attgttgcgt tgctgaagga aggaatcggt cgctcgattt
     3301 tgtctaagcg ctctgttcca gaaaatgtgc caatgactcc gttgaatttc catcgatatt
     3361 tttatttcat ccaacgaacc aatctggttt ctgatcagct gctagagatt gccgaagaga
     3421 tccttcgata caatgaaagc acagaaactt ctcacagcga ggagaatctc agctggtaga
     3481 taattgcatg tatcaaaaaa agttctatga tacactaaag ataagcagta ggcttttgta
     3541 ctgcatttgc tcaagcaact gcacaaacgg aaagttaatt tttcccaaat gtctatggaa
     3601 agactttcca ttatctaact ggaccgctat aataatcagc agtatggttt ttaagaaaag
     3661 ggatgaaaca tggaaaaaaa attaaaagct tcgaacgata atcgtattga ttatggcgtg
     3721 attttaccag tctttctgtt atgtttgatt ggtctgctct cattatatgt agcacttagc
     3781 catgatccga acaatccagc agtcttgaaa ggcgtaggga tgcaagttgt ctggtattta
     3841 gttggagcgg cagcaatcgt tgtcatcatg catttgaatt ccaaatggat ctggaaactg
     3901 actccttacc tttatggttt ggggttagtg gtaatgggac tcttgctcaa attctatgat
     3961 cagaatttag ctgcttcaac agggtctaaa aactggtttc gattcggtgc gtttactttc
     4021 cagccagcag agttgatgaa gattgcgtac atcttgatga tggcactgat cgtaactaaa
     4081 cataatacgc aagtaaaaga acggacgatg aaatcggact tttggctgat tgggaagctc
     4141 ttgatcgtga caataccggt tttagcattg atcatggccc aagatgactt tggaacgatg
     4201 cttgtctttc ttgcgatctt tggcgggatt ttcctgatgt ctggaatctc ttggcgcatc
     4261 atcgcacctg tagtaatctt ggcagtagtc gttggagcag gaacgatctt cttggttacg
     4321 actgaaggcg gaagagactt gctttataaa gttggattca aatcttatca gtttgcacgt
     4381 attgattcat ggctggaccc tttccatgat acgtcaggaa tgagttatca gccggcccaa
     4441 ggacttttgg caattggtac cggtggcttg tttggtaaag gattcaacgt aagcaatatc
     4501 tatgtgcctg ttcgtgagtc cgatatgatt tttaccgtta tcggtgaaaa cttcggcttt
     4561 atcggtggtg cattcgtcat tttcttgtat tttattttaa tctatcgtat gattcgggtg
     4621 tgtttcgata cgaataatga attttacgcc tatatcgcaa gcggtttgat catgatgctg
     4681 ctgttccatg tctttgagaa tattggagca aatatcggat tacttccttt aacagggatc
     4741 ccattgcctt tcattagtca gggaggatcc tcgattttag ggaacatgat cggtatcggg
     4801 ttgatattat cgatgagata ccaaaatgaa gcacctgcaa gatcttcaag gaggtaagaa
     4861 aattgtacac cttttactgg tacccgaaat gttcaacatg caaaaaagcc aaagcgtggt
     4921 tagaccagca tggcgttagt tatcaaaccg ttgatatgat caaagaagta ccgaaggcct
     4981 caacgattga gacttggcta gaaacgagtc aagtaccaat ccgtcgcctg ttcaacacaa
     5041 gcgggatgaa atatcgggaa cttggactga aagatcaagt aggcgagttt acggcttcaa
     5101 aagcgagcca agtcttatcg acagatggta tgctgatcaa gcgaccactg atcttacgag
     5161 aggaccagct tgttgcaatt ggattcaatg aaaaaattta tgaaggagaa ctgatataaa
     5221 atggcaggca agcatttgaa gaaaaaggac aacctttgga tcttatttaa tggaaaagaa
     5281 tactgtgttg gtttgacaaa tgaagcgcaa gaagaacttg gagacatcac gtttgccaat
     5341 ctgccaaaag caggacaatc gctaaaaaaa gatgaacctc tgatcgaagt tgaagcggaa
     5401 aaagcggtca atgaatttgc cagcccgcta acgggtacag tttcttctgt taatgataaa
     5461 atcggtgaag atatcaatat cttgaatgat tcagatgaaa tgaatgcgtg gatcttaagt
     5521 ttcaaagatg ttgatccaaa agaatttgac gaattataaa atcagaaaat cacttgaaaa
     5581 aagttgacac tgattcttcc agttgctata atcataagca atcctatatg aaacgagaaa
     5641 gctttgaaaa gaagagtaca tgtcacggac gtttgaagag agccctgttt gctgaaaatg
     5701 ggtaacgaaa atgagatgga agatggtctt agagcagagt aagcgagcgt aagcaaactt
     5761 actacgggag tgcccgttac agcgcgacag tatacggaaa gaagctaagt ccaagtactg
     5821 gaagaggctg ttattgcgaa gtaacggtaa atcaaggtgg taacacggaa gttcaagctt
     5881 tcgtcctttt gcttaaggca agagggcgaa ggcttttttg tttgaaaaat cataagagaa
     5941 agatgtgaat ggcatgtcat taatccaact aacgaatgtc aaaaagacat tcaatgggaa
     6001 aaacggaaac atccaagccg tcaatgacgt ttcactaaac gttgaaaaag gggatattta
     6061 tggaatcgtc ggctattctg gcgcaggaaa aagtacatta gtccgtctcc tgaatggact
     6121 tgagcttccc acgagcggcg aagtgatcgt aaacgatcaa gatattacaa aattaaaaaa
     6181 caaagaatta cgtacattcc gaaagaaaat cggtatgatc ttccagcatt tcaacctgtt
     6241 atggtcaaga actgtacttg aaaatatcca gctgccgctg gaattagctg gcgtaccaaa
     6301 gagcaaacgt aaagaacggg cagaagaact gttgcgttta gtcgggttag aaggacgcgg
     6361 acaagcatat ccttcacaac tttccggcgg acagaaacaa cgcgtcggta tcgcacgagc
     6421 attggcaaat gatcctgaga tcctgctttg tgatgaggct actagcgcat tggacccgca
     6481 aacaacagaa gaagtattag acttattgtt agcgatcaat aagaaattga atttgaccat
     6541 cgtcttgatc acacatgaaa tgaatgtgat ccgcaaaatc tgcaacaaag tcgctgtgat
     6601 ggagttgggg aaagtcgtag aagaaggaga cgtattgact gtcttcagac aaccgaaaaa
     6661 agaagtaacc aaacgctttg tacaacaaga catcgaacca gaagctgatt cgacagaact
     6721 attagctgaa ctcatcaaag aaaatccaag cggccgtttg gcaactttga cctttagcga
     6781 aagaaatgcc aacgagccag tcatttccca agcgattcga cgctataaca tcgatgtcaa
     6841 cgttgtgtat ggaaagatca aacaagtgaa agaaggttcc tttggttcat tgactgtctt
     6901 gatgactggt gaaaccaaag aattagatca agcagaagtg ttcatcaaag aacaaggagt
     6961 aggaattgag gtgatccatc gtggataaaa gcttttcaga aacatatcta gattttaaac
     7021 aaatcaaccc ggaaacgatg aatcaggcta tccttgatac actatatatg acattaatct
     7081 caatggtctt agtgacgatc atcggattag ttttgggatt gatcctttac tctattggac
     7141 gcaaaaacaa accgtatgct cgaatcattt atggaatcgt atcgatcatc agtaatattt
     7201 tccggtcaac gccatttatg atcctgatgg ttttgatcat tccatttact aaagcaatcg
     7261 taggcactat gctaggtgcg caagcagcca ttccagctct tgttttatcc gcagcaccat
     7321 tttatgcccg tctagtagag attgcttttc gtgaagtcag ccctggtgta ctggaagcag
     7381 cagatgcaat gggagcaagt tactgggaaa tcatctggaa ggtcttgata cctgaaagta
     7441 ttcccgcatt gatttctgga ttaacagtta caacgatctc gatgatcgga tttactgcga
     7501 tggccggcgc gatcggcgca ggtggacttg gaggtcttgc ttggcaggaa ggctaccagc
     7561 gtggtaatct aacagtgaca ttcgttgcga ctttaattat tttactaatc gtatttatcg
     7621 ttcaaggaat cggtgatttc ttaactaaga aaacagacaa aagataatag gatatagggg
     7681 gatgtaggat atgaaaaaga aaatttttgg atttgcagca gcgcttttgc tgactgtagg
     7741 attagcagca tgcggtaaca acagcgacag caaagattca gcaaataaag cagacgacac
     7801 gacattgaaa gtcggcgctt caccgacacc gcacgcagag attttggaac atgtgaaacc
     7861 attattgaaa gatgaaggaa tcgatcttga aatcgtcaaa ttcgatgact atgtattacc
     7921 gaaccaatct ttggaagaag gcgacattga tgccaattac ttccagcata ttccttattt
     7981 gaacaaagaa atcaaagaaa aaggatatga ctttgtcaat gccggtgctg tccacatcga
     8041 accaatgggc ttgtactcta aaaaagtcaa agatgtttcc gaattaaaag atggtgcaac
     8101 aatcattact tcaaattctg aatcagactg gggacgcatc atcacaattc tacaagatgc
     8161 tggcttagtc aaagtcaaag atggagtaga tctggaaaca gcaacattcg atgatattga
     8221 agaaaaccct aagaacttga agttcaacca tacgatcgac ccatcacttt tagcttctac
     8281 gtaccaaaat gatgaaggtg acctagttgc aatcaacgca aacttcgctt acggcgcagg
     8341 gttgaatcca ttagaagatg ccgtattgct tgaaaaggac aactctcctt acgtgaatat
     8401 tattgcgact cgtaaaggtg atgaagacag tgacaaaatc aaaaaactga ttgaagtgct
     8461 tcaccgcaaa gaagtccaag aatggatcac agacaaatgg ggcggctctg ttaaaccagt
     8521 aaaggctgat gcaaaataag gtatggaaca aaaaagatga gaaataagtc tgtaatggct
     8581 t
//
LOCUS       DACESX010000110         8553 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.110, whole genome shotgun sequence.
ACCESSION   DACESX010000110 DACESX010000000
VERSION     DACESX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.110"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..364
                     /locus_tag="IXF88_002154"
     CDS             <1..364
                     /locus_tag="IXF88_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1508871.1"
                     /translation="EAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIKEYLIGVR
                     INRGAFLLSLTGESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKNENFKNIA
                     LDTLKKQLEKQTAVSWTT"
     gene            455..1132
                     /locus_tag="IXF88_002155"
     CDS             455..1132
                     /locus_tag="IXF88_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1508872.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            1413..2309
                     /locus_tag="IXF88_002156"
     CDS             1413..2309
                     /locus_tag="IXF88_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1508873.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(2364..3734)
                     /locus_tag="IXF88_002157"
     CDS             complement(2364..3734)
                     /locus_tag="IXF88_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1508874.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            3972..4718
                     /locus_tag="IXF88_002158"
     CDS             3972..4718
                     /locus_tag="IXF88_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1508875.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            4715..5554
                     /locus_tag="IXF88_002159"
     CDS             4715..5554
                     /locus_tag="IXF88_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1508876.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            5555..6505
                     /locus_tag="IXF88_002160"
     CDS             5555..6505
                     /locus_tag="IXF88_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1508877.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            6646..7398
                     /locus_tag="IXF88_002161"
     CDS             6646..7398
                     /locus_tag="IXF88_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508878.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            7417..>8553
                     /gene="queG"
                     /locus_tag="IXF88_002162"
     CDS             7417..>8553
                     /gene="queG"
                     /locus_tag="IXF88_002162"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ1508879.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLR"
BASE COUNT         2972 a         1524 c         1796 g         2261 t
ORIGIN      
        1 cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc gcccagcatt tagcggtcaa
       61 tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt accagtatca aggaatattt
      121 gatcggggta cggatcaatc gaggggcgtt tcttctttct ctgacgggtg aatccgtgga
      181 aagtattgcc tatcaagtag ggttcaatag tctagtcgta tttagccgaa tgttcaaaaa
      241 gagtaccgga gaaacggcta cgaactatcg taaacgtatg aaaaacgaaa attttaagaa
      301 tattgctttg gataccttga aaaaacagtt ggaaaaacaa accgcagttt cttggacaac
      361 ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa acttgtggcg gttatgctac
      421 actgtaaagc gaagactaaa aaggaggaaa agggatgcgt acacttatca tcattagcca
      481 tccaacgatc gaagaatctt taaatcagca atttttcaaa gaagcttctg ctggtttacc
      541 agctacttgg catcatttgg aatcaagtta tccagacgga aaaatagata tagagactga
      601 actacagctc ttggcccagc atgatcggat cattttccag tttccgtttt actggtatag
      661 ttctcctgct catttaaaaa tttggcaaga taatgtcttg gaacgagcag cacatgtatt
      721 aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg gcggaaaaag aataccaagc
      781 tggaggaaaa gaagaatata cgatttccga atttttgagg ccgtatcagc gaatcgcaaa
      841 taaattccat atgacctatc ttccgccatt cgttttagca caatttatgt atttatccca
      901 ggaaaaacgt tgggagaagc taattgctta tcagcagtat ctctcattgg aaggcaaacc
      961 atcgctgact cagcggattg actggtttat tcaaagagta caggaaaatc agaaaatgca
     1021 ggaagaagaa tctgaaaaac aagcatatat cgtagaagca ctgactgagg caaaagagca
     1081 gatcgaagac ttgaccttta cactgcaaga aatgaaagga acttcattat gacaaatgat
     1141 tggctgatcc aacaattaga tgaattaagc gaaaaatcag aatatcaaga ccgtgcgctc
     1201 ttatatgaaa taaagaaact gatggctgag atagacgaac gaacaatgca gctgcaagga
     1261 gaaatcgacg gacaatcttg gaatcatctc aactggtgaa attttcttat aaataacaaa
     1321 gataaaaatg tgcacagcga caattatttg ctacaataag aatatgaagc gtttgataac
     1381 gctattatac ataaaaattg gtggtgaaaa aaatgtatga tgaaaatgag tggggagaaa
     1441 atgttcggat gacccgaaga agacagcaaa aacaggcagc tcgtaagcgg atgatcatga
     1501 ttggggcagc agttgccgtg tttttgatca tagcaggagg agttgcgggt ctatattttc
     1561 atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac ggaaacaacg acaaagaaaa
     1621 cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa agagacacaa acttctgaag
     1681 aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat cgtaaatgat tattcgaaaa
     1741 agatcgaaga aaagacgcct gcattgattg aagaatacca gacagaaatt cagggaaatc
     1801 aagagggaat agctggttta tcagcagttg ctaaccaaaa agcacgtgag cttcaagcaa
     1861 tctccgacga agggatcaga aaattacgtg cagcgtatca ggcagcagaa aataaagacg
     1921 gtgtagactt agatactttg atcaatcaat tatcggctaa ttatacgaat catgtcgcta
     1981 agatttccga tatttatttg cagactagcg cctctcttca agcagagtca acaagcacac
     2041 aagacacaac atcttctgat tctgagacag cagagtcggt agaagcttct gaaatgcaag
     2101 ctcgaacaac acaagacaca acagattcct ctgaaactaa taccaacagc gcgacttcac
     2161 aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa tcaaatagcc gaacgaactg
     2221 gcgtaccagt agaaacgatc ttgtcgctga atgggatgag catggagaat tatttcctga
     2281 ctcccggtga gacgctaaaa ttgaattgat aaaagcaata aaaaagaacg aagcttccac
     2341 taaggtgaag cttcgttctt tttttatttt ccggttcttt tttcgcgtac aaagtaatgg
     2401 atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg agattctcgt ttcaggattg
     2461 ataaacatga aaatcaatac gatagctaaa aaagcaagtg tcacataatt cgtataagga
     2521 gatagtggca ttttaaaagg atgatcatgc attttatctg cgtgcttctt tttgaagcga
     2581 atctcgctga tcaaaatcac tacccaagga accataccag gcaaaatact tgagctgtat
     2641 acgaaaacga aaatattttc tgactggttg atgaacattg gtagaagata atttaagatc
     2701 agaccaatta agattccacc tgagattgct acgacaggat aaaaaggcgc accgtgacga
     2761 gatagtttca gaaatttttt agacatcttt ccatttatgg caagtgtata gaccatacga
     2821 cttgcgctga aaatccctga attgcaacct gataatgccg ctgtaatcac gacaaaatta
     2881 atcagtcctg cagcaaatgt aatgccgact ttagaaaaag tttgtacaaa aggtgaacca
     2941 atctcactca attgattcca cggatagata ctcacaatga taaaaatagc accaatataa
     3001 aaaatcaaga ttcgaccgat agtggactgg acagcttcaa tgatcgtctt tttaggattc
     3061 tctgcttcac cagcagtcat tccgatcagt tcgattcctt gataggaagc aacaacgatc
     3121 gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga atccaccatt tttccaaaga
     3181 tttgaaatcc ctactgcctg accatgatta ccaaatccaa ataagattac cccaagacct
     3241 gcaatgatca tcaaaatgat cgttatgact ttgatgatcg aaaaccaaaa ttctaattcg
     3301 ccaaacgcct tgacagaaat aagatttgcc agcataagaa acaagacgac aatgattccg
     3361 ggaataattg ccggcaaatc aggccaccaa taattcatat aagatcctac agcaatcact
     3421 tcacttattc ctacgacaat atattgaaaa atattactcc aagcggtcaa gtagccagcc
     3481 acaggatgga tgtactcgct cgcgtaattt gcaaaagaac cagtcgaagg atcaacgtaa
     3541 agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga atcctgcaat cgcataggct
     3601 cccatcactg agggccctgt ccaacgtatg gttgaggagg cacccataaa cagaccgaca
     3661 ccgatcgttc cacctagtgc gatcatttgg atatgacggg cactaagact tcgagttaac
     3721 ttttcttctt ccatacaaaa actcctttcg ttgttgagtc aacaagaaaa aataatcaaa
     3781 cgtttcaaca catgtttttc attattaggt tagccttatt gaactcttga tgtgattcta
     3841 cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa ctttttcatc attctacttg
     3901 aaaatgcttt ccattttagt taaactatat ttaaataaaa agttaggtaa acctaaaaag
     3961 gagtgtaaga tttggaacgt acggccattg taattcaaga cctttcagcc gcttatcaag
     4021 ggaaaactgt tttgaaaact atcaacttga cgataaatcc acaaaaaatt accggtatta
     4081 tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc tttgctagag ttgataccag
     4141 ctactactgg taaagtcacc ttttctggac agtcggtaaa ctcagtccgt aaaaaaatag
     4201 cctacgtgga acagcgaagt gaattggatc tttcctttcc aattgatgta ttaggcgttg
     4261 tacttttagg aacatatcca tctttacgaa ttggacaaag acctgggaaa aaagaaaaag
     4321 aacgtgcaag acaagctttg aaaaaagtag ggatggaaga atatgccaaa agacagatca
     4381 gcgaactatc gggtggacag cttcagagag tttttattgc aagagctcta gcccaaggag
     4441 cagaatggat ctttttagat gaaccattcg tagggattga tgcgttaagt gaacgaaaga
     4501 tctttgacat cttgcaggaa ttgaagaatt caggaaaaac gattttgatc gtccatcatg
     4561 atcttcataa agtagaagaa tatttcgatg aggttattct tttaaataaa cagctgatcg
     4621 cttccggtcc agtacaagaa gtctttacat cgaagaacct tcaattggcc tatggtgaga
     4681 tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc caatccttta ttgatggatt
     4741 aatgaattat caattcctgc aatacgcttt ggtcacttcc atgcttgtag gacttgcttc
     4801 tggagtcatc ggttctttta ttattttacg agggatgtct ttgatgggag acgctatctc
     4861 tcacgcagtt cttccaggtg tagccatttc ttatatgttt ggttttagtt acatctttgg
     4921 agccactgct tttgggatgc taacagcagc agtgattggt ttcgtgactc agcggagccg
     4981 attgaaaaat gatacagcga ttggaattgt ctttagctcc ttttttgcat tagggattat
     5041 cttgatttca tatgcccaga gtgcgacaga tttgtatcat attttatttg ggaatgtctt
     5101 agctgttagg gaaagcgatt tgttgctgac agcactcgtt agtggaatcg tcttgatatt
     5161 tgtctttttc ttttataaag aattgaagat cacatcattc gatccgacaa tggcaaaagc
     5221 ttatgggctg aacacatcgc tgatccatta tcttttgatg ttctttttga cattagtggc
     5281 tgtagtcagt ttacagacag taggaacaat cttggtgatt gccatgttga tcacaccagc
     5341 cgccacggct tacttgctaa cgaaccattt actgaaaatg atcattacag ctgcaggaat
     5401 cggtatgcta agtgcagttg tcggtgtgtt tttcagttat agttacaatt ggccatcagg
     5461 agctacgatc gtgttagcat gtaccgcatt ttttatcctt gcttttttat tttctccaac
     5521 aaaaggaatt ttatttcaga aaggaagcag atagatgaaa acaaaaaaat cactcttttt
     5581 aatcttggca gttagtttct tggtattagc cggttgcgga aagcaggctt ctgaccaagc
     5641 ggatgaagga agtaaagaaa agttgtcagt cgtggctacc aattcgatct tggcggacat
     5701 ggcaaaagag gtcggaacag atcaaataga tatccacagt atcgtaccag tcggaacaga
     5761 tccgcatgaa tatgaagtac taccagaaga catcaaaaag gcaagcgatg cagatgttat
     5821 tttatacaac ggtttgaatc ttgaaacagg taacagctgg ttcgataact tgatggaaac
     5881 ggctaaaaaa gaagaaggaa aagattattt tgcagttagc aaaaatgtag aacctcttta
     5941 tttaactagc ggtgaagaac atacaaaagc agatccccat gcttggctag acctatctaa
     6001 cggaataaaa tatgtggagg aaatcgcacg tatattctct gaaaaagatg cagaaaatgc
     6061 gacactctat aaaaaaaatg cagaagcata tgtggaaaaa ctaaaagaat tagatacaca
     6121 agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa ttattagtaa caagtgaagg
     6181 ggcattcaag tatttttcac gagcatatga tttgccagca gcttatatat gggagatcaa
     6241 tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc attgatcaga taagagcaaa
     6301 agaagtaccg gttttattcg tggaaaccag tgtagactca agaagcatgg aacgggtagc
     6361 aaaagaaacg ggtttgaaaa tctatgataa actgttcact gattccatag caaaagaagg
     6421 agaacaagga gattcttact atcagatgat gaaatggaat attgaaacga tccatgaagg
     6481 tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta gcttgaggag cagaccttgc
     6541 attttttata agagatatga aaaaaaatta tgctgttttt cgtcgtacag ataccttttt
     6601 tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg tggctatgaa ttttacacgt
     6661 aaagataaat atacgattaa agaagaagtc tttcgattga tcagccagca ggaaaatacc
     6721 gacaaggatc aagaccttca atttcttgaa gcagaattag ctaaattatt catagcaaat
     6781 caaaatgaga caacagaacc agatgaagag ctgtacatag gcaaatggtt gtttgatgaa
     6841 tttcagcctt tagatgaaaa taccggtatc ggttattttg tgttcctttc tccagtcggc
     6901 ttcacacctg gcgtattagt taaacaaaac gacgaaataa ccgtcaaagc atggcctgca
     6961 ttattttcag tcatcaaagc agaaggagta atcaaaaaga tccagtcggc ctattataaa
     7021 agtcaattta ttgccaacag caaattaatg aatatgtttt ataatcgtct agtagaaatt
     7081 gctaggcctg ggaaaatccc gcaaacattc acgtacacac agcaaaaaaa tctctatgaa
     7141 gaagtattgt attcccgcga tctgtctgag ttttcgccat gggaagaggt aaggctccat
     7201 gaactgttaa gtacaaaacc agtttcattg aattttttta atgcattaga taacaaaaga
     7261 aaattaattt caacaaatta ttgggaagta tttgctgcaa gaaaaaaatg gatggaagaa
     7321 aagaagattg ccgatcagca gatccagttt gtccagctga aaaaagaaaa actatattat
     7381 gggttataca gccgttaaca gcaagcgagg aagaccatga cattaaaaga agaaatcata
     7441 caagaaagca aacgattagg aattgacaaa atcgggttca ccacggcgga accttttgat
     7501 tcattaaaag aatcgttgga agaacaaaaa gcagctggcc atacttctgg ctttgaacat
     7561 ccaaatatcg atgagcgtct ttatccagaa atgacatttg atcagccgaa atcgatcatt
     7621 tccattgcac ttgcgtatcc tactaggatc catgaagaag tgcctaggga tgaaaaacgc
     7681 gggcagtttg ctcgtgcttc ttgggggatc gattaccatg atatactcag agaccgtttg
     7741 aaccgattga tcgaattcat ccaaacaaga gcagaaaaat ggcaaaaaga agaggaatgg
     7801 cgtctagctc cgcaagtaga tactggagaa ctgatcgatg tcgctgtagc acagcgagct
     7861 ggtttaggat ttattggacg aaacggattg ttgatcactg aagagttcgg ttcatttgtc
     7921 tatcttggtg agatcgttac taatattgta tttgagcctg ataaaccagg agaatttggt
     7981 tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa aagcattgct tggtgacgga
     8041 agaatgaatg cccagcgatg tttgtcttat caaactcaga caaaagggat gatgccggaa
     8101 gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg atatctgcca gttagtctgc
     8161 ccttacaatc aaggaaaaga ttttcatttt catgaagaga tggaaccaaa agtagacgaa
     8221 atatatccaa aattaaagcc aatgctttcg atgtctaata aagacttcaa acagcaattt
     8281 gggcatttag ctggttcatg gcgtgggaag aaacctttgc agagaaacgc attgattgct
     8341 ttagctaatc taggtgatcg aacggcattg cctgagattt cagaatgttt aacagatgtc
     8401 cgccctgtca tcagaggaac agcagcttgg gctattggac gtctaggaac aaaagaaccg
     8461 gaaaaatggc ttgatgtatt agatgaaata ttagctaaag aaacagaaga agtcgttata
     8521 aacgaattgg aacatgccca gaaattattg cgg
//
LOCUS       DACESX010000111         8541 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.111, whole genome shotgun sequence.
ACCESSION   DACESX010000111 DACESX010000000
VERSION     DACESX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.111"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..888
                     /locus_tag="IXF88_002163"
     CDS             <1..888
                     /locus_tag="IXF88_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1508880.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(928..1620)
                     /locus_tag="IXF88_002164"
     CDS             complement(928..1620)
                     /locus_tag="IXF88_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1508881.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            1705..1881
                     /locus_tag="IXF88_002165"
                     /pseudo
     CDS             1705..1881
                     /locus_tag="IXF88_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(2057..2626)
                     /locus_tag="IXF88_002166"
     CDS             complement(2057..2626)
                     /locus_tag="IXF88_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1508882.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2700..3023)
                     /locus_tag="IXF88_002167"
     CDS             complement(2700..3023)
                     /locus_tag="IXF88_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508883.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3177..3989)
                     /locus_tag="IXF88_002168"
     CDS             complement(3177..3989)
                     /locus_tag="IXF88_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508884.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4343..5191
                     /locus_tag="IXF88_002169"
     CDS             4343..5191
                     /locus_tag="IXF88_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1508885.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            5336..6277
                     /locus_tag="IXF88_002170"
     CDS             5336..6277
                     /locus_tag="IXF88_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1508886.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6267..8438
                     /locus_tag="IXF88_002171"
                     /pseudo
     CDS             6267..8438
                     /locus_tag="IXF88_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2664 a         1632 c         1521 g         2724 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt
      961 gtcatgaaaa tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat
     1021 catgacgtat actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac
     1081 atttcctaaa gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac
     1141 tctagctagt tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt
     1201 agcaatgtgc catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat
     1261 gtgggcgtaa tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc
     1321 aattactggt ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc
     1381 ataggctagt ttttctttac ttacacctat ttcagaaaga taaccgtaag gatctgtatg
     1441 atcgccccaa atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg
     1501 agtgtctaat gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta
     1561 tactttatag ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac
     1621 aggacttttg gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg
     1681 aaaacaccaa cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta
     1741 gaagatatca aaggtttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat
     1801 atttctcccg aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa
     1861 ataaaaaagc actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa
     1921 ttttatttat aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt
     1981 atcttaaagg cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag
     2041 tgcgaacatg gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca
     2101 tcaattgaga ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg
     2161 gttttcttct taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca
     2221 cgactccgcc aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta
     2281 gattttttgc tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct
     2341 cttccatccc ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta
     2401 tttcttttgg ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca
     2461 agagcgtttc gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata
     2521 tatatacgcc agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca
     2581 cttctgtgat cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt
     2641 tactaatcgc tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt
     2701 tatcggtttt tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa
     2761 ggatggtaca gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcgatg
     2821 atcactagat ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt
     2881 tttcgatgtc ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca
     2941 tagcgaaaaa aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc
     3001 tggagtgttt tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg
     3061 atcgctagcc gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc
     3121 tgataataac gaacttcttc tatcagccgg tcatggatac caattacgta acgaagtcat
     3181 tcttggtaaa gtacttttgt cagctttgat ttgctttcgc tctcaaattc gctattcagc
     3241 caaaacagcg aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct
     3301 tctatccgtt tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga
     3361 taattttttt cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat
     3421 tcaaagaata gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc
     3481 ttcaataatt ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc
     3541 ggaaaatctt tcaaaaagca aaacagcaga tatcttactt ttctttctga accaaagatc
     3601 tgtgcttttt tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct
     3661 tgcgtagaac gatcgtactt ctgctcgtaa ggttcattca atagcagatt tttcagtaaa
     3721 caaaacgggg gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga
     3781 tcataaacta actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc
     3841 gccaaacgtt tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa
     3901 tcagcttttc gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca
     3961 ttttttgata atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat
     4021 tgtttgatca aaatgggttc actcctaaaa agataaaaaa ctccctttct atttatttaa
     4081 atacgcaaat cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa
     4141 aacgaatgta ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc
     4201 cattacaatg aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat
     4261 agaagaatcc aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg
     4321 caaacgttca actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt
     4381 cgaagatatt cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca
     4441 gggaaccttc agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt
     4501 ttccgtatac aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac
     4561 gatcttgtta gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga
     4621 agcaatggcc gagaatcatg caacgatcga caacaataca taccctctag acaatcattt
     4681 ttttgtctta gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc
     4741 acagctcgat cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact
     4801 tttgctactt ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag
     4861 ttatgaaata gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc
     4921 atcttatgcc ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat
     4981 cagtccacga ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg
     5041 gagaaattac gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg
     5101 cctgattttt tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct
     5161 tgttgcacaa attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag
     5221 attatttcat taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg
     5281 tattcaacaa ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga
     5341 gcttgatttc attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac
     5401 tcgtttatcg caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt
     5461 ctttacctct ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga
     5521 gattttgatt cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag
     5581 aggatgccat gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag
     5641 aaaaagtgca aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat
     5701 tcaagcagaa aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag
     5761 aggtcttgat tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga
     5821 ttggaaattg tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga
     5881 agaatgttct cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct
     5941 agacttgtat ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt
     6001 aggcaaccgc gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc
     6061 tggcgatgag aaaacttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct
     6121 ctttgttcct gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg
     6181 aacggtttat cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat
     6241 atgttcattg ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg
     6301 tttactcctt tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc
     6361 aaaacagcta ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc
     6421 ctaaaaaatg ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat
     6481 attttccttc gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag
     6541 aggaattctt ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt
     6601 ttctcataat gttgctgatc tatttattta ttacattgac cgtggattac gactattggt
     6661 acgcaccctt tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag
     6721 atttactgta cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc
     6781 aatttttctc catccggttg ttgtcgagaa acactcgatt ccagtcattg actacagttc
     6841 tgcttatttg tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc
     6901 aatctctcga ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg
     6961 acacactcca tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg
     7021 aacagcttgg tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag
     7081 gaaatggcta ttggaagatc gaaaataaaa atcattacac cggttctgga tgaatagaag
     7141 atcaggacgc acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa
     7201 ccgttataga agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt
     7261 atccttatgg aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact
     7321 atttgcctga aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt
     7381 tacaaacaga aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg
     7441 atgatttaga aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg
     7501 aggacttggc ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg
     7561 tctaacagta tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata
     7621 ctccaaaaga acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg
     7681 acaacttttc aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga
     7741 caaaaggttt tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca
     7801 gcattctcaa tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc
     7861 ttccatttga accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa
     7921 caaccgaaac cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa
     7981 ctatttccag tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt
     8041 ataaaatcgt cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt
     8101 tattcagaaa atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta
     8161 ctaatacgat tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac
     8221 ccgcagaatc tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc
     8281 gtttcattcg attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg
     8341 taaaattcga taaaaaaacc tatttagaaa taacgcatat aataagaaaa acggtataca
     8401 aaaaaattct gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta
     8461 cttctcgaat gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac
     8521 acgttttttg tcacaacctt t
//
LOCUS       DACESX010000112         8461 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.112, whole genome shotgun sequence.
ACCESSION   DACESX010000112 DACESX010000000
VERSION     DACESX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.112"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            142..2751
                     /gene="clpB"
                     /locus_tag="IXF88_002172"
     CDS             142..2751
                     /gene="clpB"
                     /locus_tag="IXF88_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1508887.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2902..3573)
                     /locus_tag="IXF88_002173"
     CDS             complement(2902..3573)
                     /locus_tag="IXF88_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508888.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(3566..4060)
                     /locus_tag="IXF88_002174"
     CDS             complement(3566..4060)
                     /locus_tag="IXF88_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1508889.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4080..5300)
                     /locus_tag="IXF88_002175"
     CDS             complement(4080..5300)
                     /locus_tag="IXF88_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1508890.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5447..7282
                     /gene="lepA"
                     /locus_tag="IXF88_002176"
     CDS             5447..7282
                     /gene="lepA"
                     /locus_tag="IXF88_002176"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1508891.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7376..8458)
                     /locus_tag="IXF88_002177"
     CDS             complement(7376..8458)
                     /locus_tag="IXF88_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1508892.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2724 a         1615 c         1700 g         2422 t
ORIGIN      
        1 ttttaagatt tatataagtc gagaaaaaaa gtcaaaaaac tatgtgtata ttttgaccaa
       61 aattgaccaa agcgatataa tagtttttgt aaagatcaaa gaaataagtg atcttacctt
      121 taattaggag ggtatacctg tatgaatatt gagaaaatga ctacgacgct tcaagaagcg
      181 attgctgaag ctcaacagat tgcggtcact cgaaaacatc aagatatcga cattgcccat
      241 gtatggaaaa tctttttaca atctaatcat tttggtcgaa atttctatac ggatgccgga
      301 ttggatgtcg aatcttttga acatgaaatt gatcgtctac tggatgagta tccagttgtt
      361 tctggtggaa acgtccaata tggacaaaac ctcagccaga atttctttcg attattgaat
      421 gaagcagacc aaatacgtga aagcttcggc gacgagtttc tttcaacgga agtcgttatt
      481 ttagcgttga tgaaattaaa aaattatccg ttaactgttt atttgaataa aaacggatta
      541 tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag gaggagaaag agtgacctca
      601 caaaaccaag aagaacaata caaagcatta gaaaaatacg gcgttgattt agtacagcaa
      661 gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg tgatgtcatc
      721 cgtattcttt cacgaaaaac aaaaaataat cctgttctga taggagaacc cggtgtaggg
      781 aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt gcctgaaaat
      841 ttaaaagata aaacgatttt ttcattagat atgggtgcat tgatcgctgg agcaaaattc
      901 cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa aagtgatgga
      961 cgtatcctct tgttcatcga tgaaatccac aatatcgtag gggcaggaaa aacagaagga
     1021 agtatggatg ccggaaatct gttgaaacca atgcttgctc gcggtgaatt acatttgatc
     1081 ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa aagacaaagc gttggaacgt
     1141 cgctttcaaa aggtattagt caaagaacca acagtggaag atacgatcag tattctgcgt
     1201 ggattaaaag aacgttttga gatccaccat ggtgttaaca tccatgacaa cgcattagta
     1261 gcagcagcta ctttatctga ccgttatatt actgatcggt tcttaccgga caaagcaatc
     1321 gatttgatcg atgaagccag tgcaacgatt cgggtagaaa tgaactctat gccaacggaa
     1381 ttagatcaag ttactagacg tttgatgcag ttggaaatcg aagaagctgc actgaaaaaa
     1441 gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt agctgaactt
     1501 cgtgaagaag caaatgcgat gaagatgcag tgggaaacag aaaaagaaga agtcaattct
     1561 gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg aattagaaga tgcagaaaat
     1621 aactatgatt tagaacgcgc tgctgtattg cgtcatggta cgatacctca attagaaaaa
     1681 gaattgaagg aattagaagc taaagcaaaa gacagcgaga ttaagatggt tcaagagtcc
     1741 gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa cgggtatccc cgtaacgaaa
     1801 cttgtagaag gtgaacgaga aaaactaatt aaattgaatg aaacacttca taaacgagtg
     1861 attggtcaag atgaagcagt tgatgcggtc agtgacgcgg taattcgttc acgggctgga
     1921 ttgcaagacc caaatcgtcc tctaggttct ttccttttcc taggaccaac tggtgtcgga
     1981 aaaaccgaat tagcgaaagc gttagctgag aacttgtttg actcagaaga tcatatggta
     2041 cggatcgata tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt cggagctcct
     2101 ccaggctatg taggttacga agaaggtggt caattaacgg aagctgtcag acgaaatcct
     2161 tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt caatatcttg
     2221 ttacaagtcc tagatgacgg acgcttgact gattcaaaag ggcgagtagt agatttcaaa
     2281 aatacggttc tgatcatgac cagcaatatc ggttcacagt tactgctgga aggcgtgact
     2341 gctgatggaa cgattccaga agcagtggct gaacaagtca acacgctatt acgtggaaac
     2401 ttcaaaccag aattcttgaa ccgaatcgac gacacgattt tatttacacc attgagccta
     2461 gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg tctggaacat
     2521 caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt ggattgctga aaatgcttat
     2581 gaaccagcat atggtgcaag accattgaaa cgatttatca ctaaggaagt agaaacacca
     2641 ctagctaaag aaatcgttgc aggacatgtt atgcctaaat caaaagtaac gattacttta
     2701 cttgatggac aacttcattt caaaacagaa gagctagaag aaatcgtcta atcttccatt
     2761 gagactataa aagaaaaaag gggtctgaca caactagtgt cagacctcct tttttcaaat
     2821 aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg aagctgcccg
     2881 cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat aactttcaaa
     2941 gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg cagcacctat
     3001 cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga caaacagtgt
     3061 gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc ataagaattc
     3121 ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata agaggaacaa
     3181 ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca atccatagcc
     3241 aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa aagagatgat
     3301 actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa accagtaaaa
     3361 caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt gtggcatgaa
     3421 aatcaatgca atccctaata aagattccac gataaatgtg attccttgag aagtcgaata
     3481 tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa aagcaagtaa
     3541 agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct aaaaatcttt
     3601 ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat ttgatcaaac
     3661 ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt agtgcagcga
     3721 tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg atcactggga
     3781 tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc gtcagcccat
     3841 ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca agagcaatca
     3901 ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga ggaagataga
     3961 tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta tataacaaat
     4021 cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca tcctttttat
     4081 caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag tgacttcagt
     4141 gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg gcgataattc
     4201 atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat aggttcattt
     4261 aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt attccaagga
     4321 gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa cggaaaccgt
     4381 tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat ctgctctttg
     4441 atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc tccatgtacc
     4501 cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg agcttcttga
     4561 tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta gaacagaaca
     4621 cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc ttcattttcg
     4681 aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg gttttgttgg
     4741 gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg ctcaaccata
     4801 ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga aagtttatca
     4861 gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata caaataagag
     4921 atccattgct tattcttgcg catattatcc aatgcaacga taattagatc ttgcatcaat
     4981 ccatggttag catattctgg ataagttcca actccagtca caccacccat tttataacgt
     5041 gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag tttattttcg
     5101 tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg gataaaagct
     5161 cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa cacataggaa
     5221 agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc aagattttct
     5281 ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac agtatagcaa
     5341 aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat tactgctttt
     5401 cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg atataaagaa
     5461 aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata tcgaccatgg
     5521 gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt ctcgcgaaat
     5581 gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca cgattaaatt
     5641 aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct ttcacttgat
     5701 tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg ctgcctgtga
     5761 gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat tggccaatgt
     5821 ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa tcgacctacc
     5881 cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa tcgatgctag
     5941 tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc tcgagcagat
     6001 cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag ctttgatctt
     6061 tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca cggacggtat
     6121 ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg atgttacaga
     6181 ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg gtgatgtcgg
     6241 ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata cagtaacgtt
     6301 agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc cgatggttta
     6361 ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag ctttagaaaa
     6421 actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag cattaggttt
     6481 tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag aacgcttgga
     6541 acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc atgttaataa
     6601 aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac ctgtaacgat
     6661 tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg attttgtcgg
     6721 tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg attatttaga
     6781 tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct ttgacttttt
     6841 cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa tgtctggtta
     6901 tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag tcgatgcatt
     6961 aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg ttgaaaaact
     7021 gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta ttggacaaaa
     7081 aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta aatgttatgg
     7141 tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga agaagcggat
     7201 gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc tgaaaatgga
     7261 cgaggacgaa ccgaagaaat agcaagggtt tgtttgaaaa cagtgtaaaa aataaaccat
     7321 gtttacatgg atttgccacc ggtttgccac cggtggtttt tttcggtggc aaaaatcaag
     7381 aattctttct aagattttcc atgatatcga ccgtttcatt tctcatctta tctgttacat
     7441 gcgagtaagt atccatcgta attgaaattc tgctgtggcc caatcgttca gagatttctt
     7501 tcatttttgc gccattttcg agaagaagtg tagcatgagt atgtctgaga gaatggaagt
     7561 taaaagagag ggagagtgca ttcgatatcc tcctagtatt ccatttcact acacttggcg
     7621 taactagctc accgtcttcc ttcgtacata ctgcatttga atcaatgtag agctttccat
     7681 acttcattcg attttctaat tgttgtttct tatgtttttt cagaattgct agcaaggttt
     7741 gtccaataaa aatcgttcga ttggaactgc ttgtctttgg cgtaccatat acccatgcgc
     7801 cgtcattctt taccatttgt ttctctacag taattgttcc atttgaaaaa tcgacatcat
     7861 cccacgtcag accacaaact tcgccaacgc gcattcccgt ataaaatcca atattcaaag
     7921 gaatatagaa aggatgtcct tcaggagtaa tttctagcat atgatcaaag tcctcaagag
     7981 aaatgatttt tagatctttt ttagtcgttg gtcgtgcttc atattttggt atttttacat
     8041 acagcatagg attttgcttg attaatcccc aaggataaac cgccatattc agcgcattct
     8101 taaggacaga gtgagtaata gtcattgttt tcttcgagta acccttttta aattcagcat
     8161 tgatgaaatt ttgtaaaaga gcaggggaga gatccgtaag ttttttcttt cctaaataac
     8221 cgtttatatg atttttgatg gtaaatcggt agttttcata ggtattgtat tttagattta
     8281 gtttaacgta ttcctccatc caaaaatcaa ggtattgttt tactcgagta tccgtaccta
     8341 aaaagtattg tcctgtttcg tcaatatctg ataaaacttt tcgtaaagca gcttcggcct
     8401 ctggtcgggt gtctccgcca actttctcca ctttttttct tgagccatca tcattgatat
     8461 c
//
LOCUS       DACESX010000113         8353 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.113, whole genome shotgun sequence.
ACCESSION   DACESX010000113 DACESX010000000
VERSION     DACESX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.113"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..724)
                     /locus_tag="IXF88_002178"
     CDS             complement(<1..724)
                     /locus_tag="IXF88_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1508893.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKK"
     gene            785..1525
                     /locus_tag="IXF88_002179"
     CDS             785..1525
                     /locus_tag="IXF88_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1508894.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(1611..2369)
                     /locus_tag="IXF88_002180"
     CDS             complement(1611..2369)
                     /locus_tag="IXF88_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1508895.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(2366..3394)
                     /locus_tag="IXF88_002181"
                     /pseudo
     CDS             complement(2366..3394)
                     /locus_tag="IXF88_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(3441..6581)
                     /locus_tag="IXF88_002182"
     CDS             complement(3441..6581)
                     /locus_tag="IXF88_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508896.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEIT"
     gene            complement(6805..7689)
                     /locus_tag="IXF88_002183"
     CDS             complement(6805..7689)
                     /locus_tag="IXF88_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1508897.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(7784..8221)
                     /locus_tag="IXF88_002184"
     CDS             complement(7784..8221)
                     /locus_tag="IXF88_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508898.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT         2420 a         1742 c         1431 g         2760 t
ORIGIN      
        1 cttttttatt ttcctgaaga gtaattttta atgaaaacag tagactttta taatcatgtt
       61 tgaattcctc tagatgatgg taatattctt catttatttg atacagttga agcgattcct
      121 gatgttgtct ttcttttact tggaaactga tcaacataat ggaaaaaaac aaagaaaaaa
      181 aactgatgac tatcgacaaa agagatagaa gcagataatt agatgagcgg aagtgaacac
      241 ttcccaatac caagtaaatc acgccccagc ttaataaaat acaaatacct ccaaaatagt
      301 gttcagttgg ataagtaaaa agcaagttga gaaatctcgt ctgtttgtct atacttttta
      361 ctaatagata gaaaataaat gcgataagta gtgcaaaaaa acttgctgtt ccaagatgtc
      421 tctttcctaa ataatccatc agatcaaaaa ccagaaaaaa gctgagtcca gtgatagcaa
      481 aaaaaagatt actgatcaaa cagctgataa acaggttttt cgtagtaaaa taaaaaagaa
      541 acatcaataa taagccaatt aggtaaggaa agctcacttt agggatagga agtaaaagtg
      601 tcaccaccat caaagctata caaccaaagc tgttttttac tagcagttgt ttcgaaaaga
      661 ataaatagat acctaaatct aaaatcaaag taaaggtcaa ataaagtgtc actgttgcga
      721 ccatcttatt cctccattgt tttgtttcaa aatgtacaca tattttttta ataggagcct
      781 gcatatgaaa aagtatttta tcatagaaga taaccagttt cacagcgaat ggatcatcag
      841 agaattggtc aatcatcata tcgagaatat cgtgcagctc acttctatct ctcaaatcaa
      901 tcagttgatc cagttgggca ctattgataa tcacgatgtt ttctttatcg atatcgactt
      961 gaagcaatca ctcacaggta ttgatgtcgc cgaacaaata cgtaaaaaaa atgcacatag
     1021 taatattgtt tttttcaccg cttatacgaa ttttgcagta gagatcatca atcggaaaat
     1081 ccttccgatt ggatatatca gaaaagacag gaatttatct gaacaaattc gtcaagtgct
     1141 agaacagatc aattggaaag aacagcagat cgtaaatgtc gataaaatct ttattgatca
     1201 aaaaggtgga agtctcgtcc tgtatcctcg ggaaatttta tatattgaaa gtatcaaagg
     1261 tttgaaaaac caagttatgg tcaaaacaac tacagaagat aaattagtga atacttcttt
     1321 aaaacaattg aagacagagt tagaaaagta tgcttacttt ttacttttaa aatcttactg
     1381 tatcaactta tcgcaattgc agaaaattga ttctgctcat tatgtgttag aatttttcaa
     1441 cggtgactcc ttactcgttg gtcgaaagat ctttgaaaag acaaaggaac gtttccatga
     1501 cttccaaaaa actgcttcga gttgatgaat aagagctgag acgtaagcat agcagatttc
     1561 agggagtggg ccttgaatcg gctccactcc ctgaagtcgt tgatatgatt ttacgcttcc
     1621 tcttctgtag gagcatctgt taacgaccat gtgaatgttg aaacgtagtt gtctacagca
     1681 taattggata caggaacaaa taattccact gattgtctcc cttgttccac attctctcct
     1741 aatgaataaa tccatgtgcc aatcccttca tttgctcctg cagttactag atctactgcc
     1801 tgatctgctg ggatttcgat tccttcagcg ccagttccag aaacggtaga tggcattcca
     1861 ctgctattag ttgcgctatt tcgaatcacg gcgtttttga atttcacctt tgaaccagtt
     1921 agtttattcg tatcagatgt tgtaccaata aattctgttc gtttgactga taatttccaa
     1981 ccggcataat ttccgctttt atccgttact tgtacgaaat tctgtacttg tgtttgtgct
     2041 ccagtactgt ccgttccgct ttgtaatgca gcatatactg tttttgctgt acctaacttg
     2101 attgttccaa aatcaagatc tggagcttgg tcaatcgtca gtaatccgga tgttccctga
     2161 tttggttcgt caggtgtaaa aggagtatca ggatcatttg gatcaacagg aggaactgga
     2221 acaacgtctt caggatttgc agcatctggt ttggtcacag aaatcgttcc ttctgtagtg
     2281 gctgagctct tattctcagc aaaaacactt tgtgtacata ataatagact tccagctaga
     2341 acggtactta atgcaattgt ttttttcatt tctttttcct tctttctttt aaaataaata
     2401 atccagcaaa taagccaata agaatgactc caacaagtat cagccatcgc cagtcaaatg
     2461 tttctggctg attttttaaa tcaatatcct tttcattcaa ttggtccgct gcatcatcag
     2521 ttatttcaaa atcggtatgg agattccagc gtttttcttt ccaagcaccc ttcacaaccg
     2581 cttgataatg tcccggagct aatttttttc cttctaatgg gacaagtaaa tcaaaatttg
     2641 tattgggtgc tacatctatt ttttcttttt ttattgaaag gtaaggctct tttgtttctt
     2701 ttttgtagat ttcaaccgat gtagtcaagc cgaacatata agaagcattt ggattttgaa
     2761 gattcgcctt aatcacattt cttccatcgc tttgatccgc ataagctttt tttaatttca
     2821 aatctgggga agactctttt tcgctatttc aaatcaagat tgctttcact tgagaaaact
     2881 gattgacgac accaactgta cttttgttag aaacgttctc ttttttttct tgaaacgtga
     2941 tccctcctgc gaccacacca gccagctctt tttctggcgc tttcagtgaa agtgtctcag
     3001 tgaccgcact ttgaggagga acagttattt ctcccactgt ttttgctaag tttttcatat
     3061 cataacttaa tgtttcatca aatttacttt ttgattctcc atattcgatc acaccgtttg
     3121 aattcgtcgt agcggagtgg atcaatgtct caactgttac tgatttatct gttcggttaa
     3181 agaccgtgta ggggatttcc tgtgttttcc caggctccat ccttacattt atatatgttt
     3241 tgttcttatc aatctgatta gccggtaatt caaaatctac cgcaaaattg aattcggagg
     3301 catggacagg tgtctgccaa aggatgaatc caaggagaag gatcataagt aaagagcagt
     3361 tgtttcgatg acttttattc catattcgat tcatcaatca tcctccttga tctttattct
     3421 gtcggcgcat ctgctaatgt ctatgttatc tctgctgagt aattaccagc tatctgattt
     3481 tctttggtta agacaagttt aaacgcccga ccgttattta aatattctga ataatctgct
     3541 tgcacattcg ttgaattttg tcctctgaca atttccagtt cttcttcatt cactaattga
     3601 tactcctctc ctttcttgaa atataggaca tcctgcatag aaaatcctgc atcatttgtg
     3661 aaaggctgag ataattttat cgttaagatc cacggatttt tcgtttctcg ctgatcataa
     3721 atccataatt tttcctgtat atccatttgg ccaaatgctt cttgtgtcaa accagttgct
     3781 ttgatctgac caaaatcgag tttggaaggg gcaagaaatg agagaccacc ttctactacg
     3841 atcttcgtag caggtgcaaa aggcggggca tcatgataaa caactggcgt atcacttgga
     3901 ttgtgattcc caatttcatc gttggtactt ggcttgttct gcacaccctt agcagtagct
     3961 tctcctgctt ggtcgtttaa tacaatttct aatacatctc ctgcaaaaag ttgattttct
     4021 gggatagaaa gaacaaattc ccctgtatca tcaatagttg tttctgacaa aacagcttga
     4081 ttttttttca atgaaaccaa ggctcctgct tcacctgtac ctgtgatcgt gcgagaagct
     4141 gcattgatca atgtggtact taaattagca ggttcaggag gaatcacatc gactgtcgtt
     4201 ttggcagagt ccagttctgt cacttcttgg ccacctctta cttttttagc agcaacaact
     4261 tgcacttgat cctccgtttt taatctctca tcattgggta cttgaacttc gaacaagccg
     4321 ccttgttcgc cttctcccca tgggttatat cctgcccctt cattttcatc tgtttgtcct
     4381 tgcgtgagag cagtaatttc tacagatgat ttcgtcgtat ctgcgtaagt aatacgtaaa
     4441 gtcacttcta cttcgcctgt ccaagcatct cttggctttg cctcgacacc ttcgggaaca
     4501 acgacatgcc catagatttt tttatctgca ttcgttggca ctcttaagga ttcaatcatc
     4561 gggagctgat tattggcact gattcgatta taatttttca ttccggcacc tgtctgagca
     4621 aattcatttg ttaaacctgc actagggctt gatacgaaat tgcctaagtc ggtcccattg
     4681 atcgataagt ctgtcatatc agaaaaataa tccgggtctt tttcaaagtt atcgaatgtt
     4741 gcgcctgttt ttcgccataa agcgatttct gaatgggcga tctttaaaga agaatctcca
     4801 tttccttgga atacccagcc gcctatgcca ttggtctcgc ttccaatccg tttgttttgg
     4861 aaatcaaaat aggtaggaga ttctaagtcc acattcaaca catccccact gatgatgccg
     4921 gtcttatctt cccctgtttt cccgaccatg actaaataag ttttttcacc tgccgtaaaa
     4981 tttaaatcaa tatctccagt tgtatctaca gttgctccgg aatctgcgcg tatatcgata
     5041 tttgattttt ctccagtgac tgtaaaatca gctgaacctg gactttctag aaaaccagaa
     5101 gcgtcataaa attggatcgc ttgatttcct tgattgagtc tgttcacgta agatttgcca
     5161 tcacctgtat ttcttaccct caatgagcct ccattggtta ctgtcagctt ttgcgtacca
     5221 ttctcaaaac gaactgcgct ggaacgccca ctctgcttat cgatgtcgat aactgatccg
     5281 gctccttcta cgatcaaaga aaggcctttt cctacaaaac gcatcccatt gttcccgggt
     5341 tgatcgtcat ctccagccat tttgaccgag atttgtccac cacttgatac atggatttga
     5401 ctagaatctc cttgcaagcg aaatccgccc gtagttgctg tattgatggt gattttccca
     5461 ccattcgata cagtgacttt cgatcctgaa cctctcattt ggatgattcc gcgattgttg
     5521 tcattggtaa tattgatctt tgtatttacc gaaaattcgc ttcccgaacc actgacttca
     5581 aacatgacat taccaggtag cgtatcgact gcctctcctt catcgctaac gccatctgcc
     5641 cgatcataat caagagagac ctttgaaccg cttaagatat ggattcctgg cgctgttttt
     5701 ccactaccag tctcatctcc tgaatctaga aaaccgtaga atttccctag atacggtcca
     5761 taaagttgac taccattgat cacattcaat tgatagcttc tacaaaaata ggctttaccc
     5821 ggagcggcat tcagtccttc cttcactggt tggacatcca acacaccgga aacaccatct
     5881 aatgtgacag ttgcacgagg agcgtaaatc agtcctaatt tggacccatc atacaaatca
     5941 agtgtcccgg taaattttat ttctcccgta aaatgatatc tgcttcccca aaaatcggtc
     6001 atataagcaa tgataatggc atttccatcg atatttggtg tcaatccacc tggatgtgca
     6061 gctgttcctt taaaattctg gaactcgatc gactgaggta tctcattgat ataaatatgg
     6121 tgtgtgccga aattgtgagc gaatccacca aaatcaataa cgatatctcg tttgatctct
     6181 aattccctgt ctaaagtcaa aggctgaatc agtcggatct tagttactga ttcgtctgcg
     6241 agaagctgcg ccagttcttc tgttgtggca gcttcgctgt acccatccgc tgaaacagct
     6301 cttgccagtg ccgcttcact gctatcagac gtttgttcag ctacttctga ggtctcactt
     6361 gtcttatttt cttcttgcct tttattagta gaagcctcac tttgagattc tgtagtttca
     6421 gaagtaatcg attcttctga aaaagtagtc ttgtcaaaac tttgattctc tgattgcgtt
     6481 gattcgtttg ttttctctac atcggcaaaa acggatgtca ccatcaagag agggaagaca
     6541 aacaaaatcg acgcaatcag cttttttaca cattgattca ttctccacca tcctctctga
     6601 cgttttgaca attttatttt atcgttttaa tctatttcca attgtacttt catatttaaa
     6661 atatgtattt ttatggaaaa atttttctat taggtgtaaa tttctttttt gtacaaaaaa
     6721 aaggctgtga caaaagtctt gtcacagtcc cttatcccga acaagcggtg ttctcccgcc
     6781 aactcctcgg tcacaacctc cattttaaca ataaacctgt aaatgtgagg gcaatacttt
     6841 gattgtcaca ggtaatttat ccccttcatc tccatcaaca ttcgtcgtca attcttcctc
     6901 atcagctaat gacacaacta attgctcaca agtaaggtag gctagatttt tcgtagattc
     6961 atctactcct tttaataggt ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt
     7021 taaataaacc aaatggagct taccatcatc cacttttgca tttggaagta atgtttcaaa
     7081 accgccgact gagttagtta gtcctatcag caaggtactg ctctcaattt cttccttctt
     7141 tccatcaact tctacgtgaa acgagtagga ttgtgtggaa gctaactgtt tgattcctga
     7201 catgaaataa gctagttttc caaatttcgt cttcttctcc gcatctacat cattgattga
     7261 ttcagggata gagccaattg cgacgacatt cataaaatag tcgtcattta tttttccaat
     7321 atccaatggc ttgactgatt caatcgaaaa atgattgatc gcttcctcag gttctagcgg
     7381 tatccctaat gctcgagcca agtcattcac cgtacccaat gggaaaaatc cgaaatttgg
     7441 ccgatgttct tgttcagcga ttccactgat tccttcgttg actgttccat ctccgcccat
     7501 gacaaacaca ctatgatacc cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc
     7561 tgcttttttt gtatgcaaga cgaccacttc atcaaacacc gattccaatt ttgctatcgc
     7621 taattgctca aattcctttg cttgttcacc gcctgaactg gggtttacca ctaataaaac
     7681 ttttttcatc actttctttc ccttctgatt gatttcttta atataacgat agcataaaga
     7741 aaaaaattag gctgtgatga agtactgttc atgaaacatt catttatttt ttgacaagat
     7801 aaaacatcca tactatgaaa ctgaggaaaa ttaaaacaat gatataggaa ataaaagaaa
     7861 atgataggca ttgtatacct aataaagtaa aaatcttagg tggcagataa aaacacaaaa
     7921 atagcaagag aatgtaatag cgtttcttcc actttttgtt atcagacctt atagcgatca
     7981 gaggaatacc aaatgcataa ctgaaaaact cttgatgtac tgttaatatg tcacggcctt
     8041 gaataataaa tgcgacaatc cccgctataa ttagtgtgcc taacaccaaa acggaaaatt
     8101 gatgaatata ttttatatac aaatgatcca ttgtacttcc tgccttttta tttgtgagaa
     8161 aaacaaatcc gtatacgtga ataacttcta gaaataaaag aaatttgcaa aaccactcca
     8221 tttatctact tctacgtgca ttttttgata ttgttttctt tatttaagat tatacagaaa
     8281 aatatctagg gtcgctggat tctaagtaaa aaaaattttt aaaataaaaa gaactaaatc
     8341 aagtagtaat taa
//
LOCUS       DACESX010000114         8053 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.114, whole genome shotgun sequence.
ACCESSION   DACESX010000114 DACESX010000000
VERSION     DACESX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.114"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(532..930)
                     /locus_tag="IXF88_002185"
     CDS             complement(532..930)
                     /locus_tag="IXF88_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508899.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKELYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1146..2201
                     /locus_tag="IXF88_002186"
     CDS             1146..2201
                     /locus_tag="IXF88_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAQ1508900.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVIEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2221..2574
                     /locus_tag="IXF88_002187"
     CDS             2221..2574
                     /locus_tag="IXF88_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508901.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2756..3052)
                     /locus_tag="IXF88_002188"
     CDS             complement(2756..3052)
                     /locus_tag="IXF88_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508902.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3056..3874)
                     /locus_tag="IXF88_002189"
     CDS             complement(3056..3874)
                     /locus_tag="IXF88_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1508903.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4100..4321
                     /locus_tag="IXF88_002190"
     CDS             4100..4321
                     /locus_tag="IXF88_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAQ1508904.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4323..4589
                     /locus_tag="IXF88_002191"
     CDS             4323..4589
                     /locus_tag="IXF88_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1508905.1"
                     /translation="MSYQVRYEREAQKTLKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4702..5241
                     /locus_tag="IXF88_002192"
     CDS             4702..5241
                     /locus_tag="IXF88_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508906.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            5732..6016
                     /locus_tag="IXF88_002193"
     CDS             5732..6016
                     /locus_tag="IXF88_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508907.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6023..6268
                     /locus_tag="IXF88_002194"
     CDS             6023..6268
                     /locus_tag="IXF88_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508908.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6290..6544
                     /locus_tag="IXF88_002195"
     CDS             6290..6544
                     /locus_tag="IXF88_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508909.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALAYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            6626..6811
                     /locus_tag="IXF88_002196"
     CDS             6626..6811
                     /locus_tag="IXF88_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ1508910.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            6878..7273
                     /locus_tag="IXF88_002197"
     CDS             6878..7273
                     /locus_tag="IXF88_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ1508911.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7263..7640
                     /locus_tag="IXF88_002198"
     CDS             7263..7640
                     /locus_tag="IXF88_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508912.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            7641..8036
                     /locus_tag="IXF88_002199"
     CDS             7641..8036
                     /locus_tag="IXF88_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508913.1"
                     /translation="MKQEKRKMKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLW
                     KYKILFLFWEATLTFSVLFYKSDCYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         2820 a         1391 c         1304 g         2538 t
ORIGIN      
        1 tagaaatata ttggtttcgc cttcagatta atactggatc atattaatct gaatacaata
       61 tatgaactaa taaatagctt aacctcgcta ttttgttttt tctgatcatt atatacacag
      121 cattaacaaa tagaatttta aaaataaaaa aagtaagaca aaaaaagtat caacataaac
      181 atatacatat acacgtttat gttgatacac ttaatttgtc ctttaaaaag cactgatttt
      241 taaattgcgt tattttataa cccgaataca gtctgcttgt taggcattgt ccttaaatta
      301 catttttcat aggttattta atcatttaat ctgttgatta ttcttgatct aaaacttagc
      361 ttaacaaata tataatcagc caaccgcctg ataaaaatgc gatcattgct aatatataag
      421 caaagataaa aagatgttcg atcagcttaa accctgatgc gattaagcag accaagtaaa
      481 atagaaattc tcttactgat aatggtaatt ttctaactag catactcata attaacaccc
      541 caaagtaata ttgtattttg aacattttac atccagtaca gcatcaatca accattgcca
      601 atcaaaaata caaggcttca ttgaagtatg tttttcagat gaaaagatat aatataattc
      661 ctttggaata atattttcag ttgttgggat caagctaaat ctaattccat ctaactcata
      721 gctaccagct ttctctccat taaaaatttc cttagcactt tcttctaaag aatcaaaata
      781 tttttcgaca gcaacctttg aagcttttac tattgaagca tttccctttt cgataaaatc
      841 tgcttttgtt ttaccgacaa taagtaattc ttgatcagta aaatcttcta aaataatggt
      901 agtgtaattc ttatttcttc tcaatcgcat caggatcact ccttaatata atttatgttt
      961 ttatgatatc ataataagat aaatttgcaa gtatttgttt acaccttttt atttacactt
     1021 tattataatt aattatactt ttatgattgc atttttttct tataaacagt tgcaactaat
     1081 agtaaatagg tgtaaactat tttcgtaccg aaataccata aacacaaaca gaaagaggtc
     1141 ataccatgaa agaaccatta ttgttttccg ttgacttagg gaataaatca gtaaaagaaa
     1201 tgagtacaca cgtttctcca ctctcacttc cctctcgtat tttgaacaaa aatatcatta
     1261 attcaggacg tttttctaat ataggttcct cattaaaacg atcactaaac gtttctgaat
     1321 atagcctagt aaacactact gaaagctatt attttgggaa ggatatacac gagctaggaa
     1381 aagatgacta cgtgatagaa tcactaggtt ttgggattag cagatacaag caggaaacct
     1441 accaaaatat cctatctttc tctttagcta acctagcacg tttagaagat caatctgatg
     1501 acgttttaac cgtcaatcta gttttaggtc tgccttcatc cgactatacc gaaacaatcg
     1561 tcaaatacgt gatgcaacaa gccacacgac aacattcagt tgaagttgac ggtaaggctt
     1621 ataatataaa cgtagatacc gtttgggtac tgccacagcc aatcggaacg ctatataacc
     1681 tcctacttgc tgatgatggt agcgtacaag atgaaacctt gatcagtcag aagatcggtt
     1741 ttgttgatat tggtggtggc acgaaacttt ttgatgttgt ccaaaatttt gaaattgacg
     1801 aaaacaatcg tgaacaaaag gataccggca tttttacttt atataatcgt atcgtaaata
     1861 tgcttgacct tccgatcagt cctaatgctt tccaacttga aaagattatc cgagaaggca
     1921 aagaaaaagg cgtatatcta tttaaaccaa gcggaaatga aagttttgat atttcagaag
     1981 taattgaaag agaaataaaa acatacacaa atatggttgt tgcagaagtt ttagctacat
     2041 tcaaaaactt cacgacctta gataaagttg tctttactgg tggtggagct tctattatcg
     2101 acaaacaact tgtaaaagat aaaattccgc ataccttgtt tgttgaagac agtgaatttt
     2161 ctaatgtaaa aggcttttac aagtttggtt taactcaata aaacagcgaa ggagatcaac
     2221 atgaataagg gaaaatcaga gcgttatgta gttataatca atagagaaaa ggttagcgag
     2281 ttacctttag cagagtttct tgaatccgaa tatgaaacac taggcgttaa atactctcag
     2341 acgatcaaaa aagctttgaa cctatatcgg cgtgtaaaga acaatgaagt tacaattatt
     2401 gaagcaggca acgatagaaa cgatagcaaa gtagcagaag taaagaataa accaactgat
     2461 cataacccta ttcaacaaga gcatccttca acggttacat ccgatcattc agcaaagaaa
     2521 gaaaagccaa tgctaaaagg gttcaatgca ccaagtaaca aagacttact ttaaaatgaa
     2581 aaaggcgagg ttacaatacc tagccttttt attatgtaca aatataaaca taaacatata
     2641 cgtatacatg tatataaaca tgtatacgta tatatccata aacacgtata tgaaatttag
     2701 ttaagctata cctaaccgct tattccaata aaaaaagtga tcagctttaa actgatcact
     2761 caccaaaaac ttcatcatac aagttatcta tcatttcacc aagtaaagct gaatctgatc
     2821 gaattcctaa cttttcgctg attttagata gcttctttct ttgcgatggt tgaagcgtga
     2881 attgataatt tttcttcttt tcagaactat cactttttag tttagctacc aaagaacttg
     2941 ttgtttcttt aggtgtttgt ttaggttctt caactgcttc aaccacttca ggaacttctt
     3001 ctacttcagc ttcagcgaat tttttaaatc tattttcttt cacttcaatc actccttatt
     3061 cttgtgccgt atcaattatc ttgttgtata gcgtaaacgt gctttcaaag aaatccttat
     3121 tcttacgata gatattctta tccttagcga actcagcaat attcttacct tctaagctag
     3181 ttgcattaag caattccttc tcttgtacat atccaagata attagataac gttggtacca
     3241 attctaggaa ctcacgactt gaattagtat tatgtttgat cctgctaccg atcgtagcgt
     3301 atttagtttc catcagtgtt tcaccagtct taatatcaat caactgtttt gctagtgcat
     3361 caatatcttt accaagcttc agcaaagctt caaaaccatc tttagatgga tcagaaatgc
     3421 ctacgacaag atcagataca gcccaagcat tttgagctag tagacctgca tcattatgag
     3481 tatcaatcag aattagatca tacttctctt taagctgttc atagttaaca acgatccatc
     3541 taaacagtag ggttctgttg ttaggcatat cacgcatttg catctcaact tgttttaaat
     3601 cttcacctgc tactaaaata tccatgttct catttatagc taaaggttca gctgatccat
     3661 gaagatataa atgattgatt gtattctctc ttttcgcttt actgaaagca tcaggaaagc
     3721 gttttgatag gttttgcgac ttatcttcat caatatacag caccttcata cctaaatttt
     3781 cgacagcata tccacctaga ttgtagattg ttgttgtttt acctgttcca cctttttcaa
     3841 tgtttccagt aatgattgac aacttctttt ccatgattaa acctccctta ttaacctttt
     3901 gtaactccat tatacactat aaacacgttt atgtatatgt ttatatacat aaacgtgttt
     3961 atatttatat tcgcttttat attctataac ataaacatat atatgtatat gtttatgttt
     4021 atgtatatgt tcatatatat aactattgca tctgtcatac aatcgtagta caatgtaatt
     4081 gaaaaggagt tgataaacta tgccagtaat aagtattcgt gtatctaaag aagaaaaaga
     4141 ttttcttgaa aaagttgctg aattcaacgg agataacatt tctgaattta tgcgtagtaa
     4201 ttccctgcaa acagcagaaa cgttagtaga ctttgccacc tacaaagaac taatgaaaga
     4261 gcatgaggaa aacgatcaaa gtatttccca cgaagatatg ctaaaagagt taggcttata
     4321 agatgagtta ccaagtaaga tatgaacgtg aagctcaaaa aacgctaaaa aaaatggata
     4381 agtttcaggc aaaggttatt ctaaactgga tcgagaaaaa tcttgtaggc actgatgatc
     4441 caagaaagca tggtaaaggt ctaacagcta ataaatcagg ctactggcga tatagggtag
     4501 gtgcataccg tataatagct gatattagcg agcatgaagt gacaattctt atattgtcta
     4561 ttggtcacag aagtgaagtt tataagtaac agacaagctg ataaggcttg tctgtttttt
     4621 tgttaaccca acaaaatcac tgtttacgat cccactttac ctaaaaaaga acaatatcct
     4681 cttgctatta tacacaccag tgtgtataat ataagtgtaa acaagctaat taactttcaa
     4741 aaggagcgat tttttatggg atggtatggt gcttattcat cagcaaatga agtaatcgaa
     4801 gaaaagaaag ggtacgccaa aaatggtttg gaaatcgtag ataccaaagt aacgaagacc
     4861 tatggaatta tccttttcaa agatgcagaa ggaacctacg atattgacag cttcatcttt
     4921 cgagatgaaa tgtacaaacc aatctacttc ttaaatgaca gtcctgctta ctttaagaaa
     4981 attccaaaaa aatggttgaa attctttgtc ggtgatcaag aaaaaatggt taagcgacaa
     5041 ctagatagaa ttaaagaaga aagcaataaa ccaaaacttg atgacttatt agttgcaggt
     5101 aaaacttact caatctggga tggatcgtat gtcgctgttt atcagtataa actcaaacgt
     5161 agctttattt ttcgactaca agatggatca ctcactcggt tcactcgact atcagcagga
     5221 gatgttaagg aagtagccta aaacttaaat acataaacat aaacgtgtat acgtttatgt
     5281 ttatgtatat gtatatgaat atcaacgtat taggtacgaa cgcttttaaa tgcttttctg
     5341 ggctttgatt ttagggtagg gctaaaacat caccatctga tccaaaacag ctcagaaacg
     5401 catttaaacg tatctgatac caatattaca gaaggagtgt atcgtatgat taacctattt
     5461 cttttagtga cagctagtac attattcttt actggaatat cactaataat ttcctatttg
     5521 atctataaga aagccgaaac ttacacaagc aaagcaaacg gactagccgt ttcatccttt
     5581 ttaactttgc tgactttttt tgttactcta tcgttttctt ttgtttagca cagctgatcc
     5641 atcagcaaaa aacctctctt tacattatac acattggtgt gtataatgta attataaaca
     5701 agctaattaa cttttaggaa ggagtgacaa tatgctaaca gctacattac agcaacctac
     5761 tcacgttgct attgcagaaa gctatacaat taatgcaggg gtcgttaaac agctgatcaa
     5821 ggatcgagaa ttcaaaaaat ttaacagtcg tcaagcgtat aacagacagt taaagaaagc
     5881 tattctaaag cacaaaaatt ccttcatcgg gaaattggaa agtgtggatg ttaaaactgt
     5941 ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt ctctatttgc
     6001 ttttggaatt aattgatagg gagtgtggac tttggaaggt aagacttttg cagaaatttg
     6061 gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa aggtattgga
     6121 tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga tgtatttaga
     6181 agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg ccgagtggga
     6241 tgcaattgga aatatccatt tttcttaata caagaaagga gaacacaata tgaattatac
     6301 cgatgaagaa caaatcactt tggaatacgt tttcacaaat gaaaaagggg aggaaattga
     6361 aaaagtcttg atcatttcat caaaagactt ggagaacgct ttggcttatg aaccatacga
     6421 atcaaccgta actgtcaatg aattcttaga tacctatact tctaatgata cttgggaaat
     6481 tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata aatcatttta
     6541 ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca atgtgtataa
     6601 tagattttgt aaagggaggg gagctatgcc taagacagca agagaaattg taaaaattct
     6661 caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat tttataattc
     6721 caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac taggaacaga
     6781 agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt ggtattaggc
     6841 atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa atatttttta
     6901 tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg acttaccgga
     6961 aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg atgctttagg
     7021 attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag tggaagaatt
     7081 aactttgaaa gccaatcaag tggtagtaat catccaacta aacattaaac ttttccgaag
     7141 aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt tagttctttt
     7201 agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag aagaagagtt
     7261 gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac tatttctctc
     7321 gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt accaaacact
     7381 ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg tttattgaac
     7441 aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa atgaaacatg
     7501 caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct ttaaacctcg
     7561 ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag ttgcttgctc
     7621 aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa acacaaacag
     7681 aacccattaa cgatacgctt gctgatcaat tagcaggata ttataaatta tttgagatga
     7741 gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt cttttttggg
     7801 aagcaacact aacattctca gttctttttt ataagtcaga ttgttataat caacacttag
     7861 ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat acctacatca
     7921 cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc aaaagggtat
     7981 ctttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg ttctgagagc
     8041 ttttgacatt ttc
//
LOCUS       DACESX010000115         8031 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.115, whole genome shotgun sequence.
ACCESSION   DACESX010000115 DACESX010000000
VERSION     DACESX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.115"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..369
                     /locus_tag="IXF88_002200"
     CDS             <1..369
                     /locus_tag="IXF88_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508914.1"
                     /translation="LLLAGNITTIWLCWQQSAPLFYLLSTLLSVITLFLVFAMLNWVV
                     IKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLGIYFRILMILPVIFLFGGTAVTVI
                     LISKLTERALAKLPCQIQSH"
     gene            399..2312
                     /locus_tag="IXF88_002201"
     CDS             399..2312
                     /locus_tag="IXF88_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAQ1508915.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            2440..5421
                     /locus_tag="IXF88_002202"
     CDS             2440..5421
                     /locus_tag="IXF88_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAQ1508916.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            5418..7079
                     /locus_tag="IXF88_002203"
     CDS             5418..7079
                     /locus_tag="IXF88_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAQ1508917.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            7119..>8031
                     /locus_tag="IXF88_002204"
     CDS             7119..>8031
                     /locus_tag="IXF88_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1508918.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCV"
BASE COUNT         2637 a         1324 c         1664 g         2406 t
ORIGIN      
        1 ttgttgttgg ccggcaatat tactacgatc tggctttgtt ggcagcaaag cgcaccattg
       61 ttttatttat taagtacatt attatcggta attacactgt tcctagtctt tgcaatgttg
      121 aattgggtcg tgatcaaaag tcttgggttg tggatgatgg gagaccgact gattgactat
      181 ttgcttttca cagcaacgat gttgccaacg gtagtcatgc aaggcttggt gcttggaatt
      241 tatttccgaa tattgatgat tttgcctgtg atttttctgt ttggcggaac agctgtaacg
      301 gtaatattga ttagtaaatt aactgaaaga gcgttagcaa agttgccttg tcagatacaa
      361 tcgcactaat tttttcacaa aaaggagtat ttctttctat gaagttaact gataaacaag
      421 tagctaatat agcctttatc cagcaacttg agattccatt gacacaaaaa gatgaagaac
      481 aaatagactt gatttttact gacacaaagg ctaatgtgcg acaaatcaag attactaaaa
      541 atgagcagca acgaatttgc ttgtcttatt cacatgaaga cttgctgtat cgaggattgt
      601 ttatctgcct tccacgtttt ttagctcaag gaggagagat gccatacatt gaagaaacct
      661 cgatcattga gaccagttat tcattagatg cttctcgtaa tggcgtgatg aataaagaga
      721 ctctaaaaaa attcatttct tacttagcgt tgatggggta caacacattg tatatttaca
      781 cagaagatac ctatgaagta gaagaccttc catattttgg ttaccttcgc ggagcgtata
      841 caaaagaaga agtgaatgaa attgttgaat ttgcttctgg ttttggcata gaagtagttc
      901 catcgatcca gacccttgct cacttgaccc aatttttgaa gtggtatgct caacaagaac
      961 tgatggatga tcaaaatact ctactgatcg gtgaagaaaa ggtttatgcg gcaatcgaaa
     1021 agatgattat tgcctgcaaa gaaatgtatc agacagagcg tattcatttg ggaatggatg
     1081 aagcctatca agcaggtctg ggacgatatt taagcctgta tgggtattgt gatcggacaa
     1141 ctttaatgat tcaacatatg gaccgtgtgt tatcaattgt tgataaatat aagatgaaac
     1201 caatgatttg gagtgatttc atttataagc tattagatga aaccggtaaa gataggtatt
     1261 acaatcctga ttttaaactc aaagcattag cagctaaagg attaccagca ggattaacct
     1321 atgttcattg ggactatggt tcagatgaag tttcctccta tgagaaagta attgataacc
     1381 atttgaaatt ttgtgacatt gatcattacg ttttagctag tggtgcccat atctggggga
     1441 gactagcccc gaatcatggg aaaacagaag caattgtttc tgcagctgta aaagcttcta
     1501 aaaataaagg tgtacgttct gtgatgttaa ctacttgggg agatgaaggg caggaaacag
     1561 agcattggca ttccttaatt ggggcattaa aattagcaga ttatgtctat tctgaagaag
     1621 agtggaatga ttttgaggaa atctacgatc gtttattagg aacaggtagt tatcaattaa
     1681 tgtccgaatt acgatatttc gatgaagtgg ataccattac aaataacaac ttacaaatgg
     1741 cttccgtgag taaactgcta ctttggcaag atccgttagt tggtatatat gactttcatg
     1801 tagccgctca taacaaaatt gccactagaa caattggtac ttattatcat gagttagctc
     1861 agaaattaga tatgaataaa aattggaatc caaaacaacc aattttaaca actattcgtc
     1921 aacgatatat acttttagct gaaattttag cacaaaaggc agaactaggc atctatttac
     1981 aacactgctg tctggtagaa gaccttgcag gattaatgga attagcgaat cggtgtgaag
     2041 cactagccaa tcgagtaaat gaattggcat ctattcatga aataatttgg tatcaaacat
     2101 acaaaaccta tggttgggaa gtattagaac aacgatatgc tgcattagct catcgtctga
     2161 aaaccgttgc gcgtcgtatt aaaaatcatc ttcaggggca agatgcccta gaggagcttt
     2221 tggaagaacg attaccgttt tgtcaatatc aagtgccgct tgaaatttct ggctttaatt
     2281 atcggcaaag ctcggtgtca ggctacaatt aacgaaggaa tcttcccgtt gaacggacca
     2341 cttgctcatc taatgggaaa gtggttcgtt ttctattcat aagtagctta ccactaactc
     2401 ttttattaag aagagaaaaa aagtaggaag tgagaaaata tgttatcgga ctattatcaa
     2461 aatctgtcag ttataagtgt caatacagaa ccattacgta attattatat tccttatgca
     2521 agtaaagaag aagcaaaaca agagttagaa agggaaaaaa cgagccagtt tatcgatctc
     2581 aatggtacat gggattttca gtattatcct tctgtccaac agttaccgga aaattttttt
     2641 caagaggcct ttactgaaag ctatgataga atttctgttc ctggagtttg gcaaacagag
     2701 ggatttgact tacctcagta tatcaatgta tcttatccaa ttccttttga tccgccttat
     2761 gtacctaatg ataccccttg tggtgtatat cggcgtcagt ttcaaattga gcaaacagat
     2821 gcacgctttt acttaaatct agaaggagca gatagtaatt gttttgtttg gataaataga
     2881 aactttgtag gctatgacca agtgagtcat agtacatctg aatttgatgt gaccaagtat
     2941 ttacaggttg gggttaatga aattcaaatt ctagtgatga agtggtgtga tggtacctac
     3001 tttgaagatc aggataagtg gcgaatgagc ggtctatttc gtccagtgta tcttcttcaa
     3061 cgaccaacta tgcatctgac aagttatcaa ctacggacgt tgactgatcc taaaaaagga
     3121 agcggaacat taatggtttc ttttcaacga tttactccta ttgaagtatc gttacctatt
     3181 tcttacgaat tattccatcc tgatggacgt ctcatagcga aaggggagtg ttactcggaa
     3241 caacaaataa atttggatgt tgaagattgc cttttttgga cagcagaaac gcctaaatta
     3301 tatacattac ttttaacatg taatggtgaa tattttaaag aaaaagttgg ctttcgagca
     3361 ctgacgatca agaatcgtaa gttattgctg aacggaacac ctattaaatt atatggagtc
     3421 aactatcatg acgtgaatca gcaaggcacg ccagtgatga cccgagagca gttacgtgca
     3481 gatttgttat taatgaaaaa attaaatgtg aatactgtac gaacggcaca ttaccctaaa
     3541 tcaccagatt tttatgaatt ggcggatgag attggtctgt atgtaatcaa tgaagcagat
     3601 ttggagtgtc atggtgtggt taatctatat gatactgaag cgaattatca actaattgct
     3661 aatgatccga cattcgaaaa gccgattatg gaccgagtga tccgttctat tcttccttat
     3721 caaaatcgaa gctctatcat tatgtggtct atggggaatg aatctggtta tggagtaaat
     3781 tttgaaaaag ctcaagcttt agcacggaaa ttggatgatt cacggttgat tcataatgaa
     3841 cgctcgattg aaccagttat tgaccaagtg aatgatttta gtaacttgga tgttattagt
     3901 caaatgtatc ccccactgga agatgttcat cgtcattgtc aaagaagtga tactgaaaaa
     3961 ccttttttta tgtgtgaata tagccacgct atgggcaacg gtcctggaga tcttaaaacg
     4021 tacttcgatt tgattcaaca gtatgattcc tttcttggcg gttgtgtctg ggagtggact
     4081 gatcatgcaa tcaatagagg aactttagaa acgccatctt atttatacgg tggtgatttt
     4141 ggtgaaaaat atcacgacgg caatttttgc gtagatggtt tattgacccc agatcggcaa
     4201 ttaacagtag gtgcattgga gtaccgtaat attcattcgc ctattcaact gattgctgta
     4261 gatgaggagc tgcaagaagt aaccttgagc aatataactg cttttacagc cggttcagca
     4321 tttaaccttt ctcttgaatg gtatatagat ggaaaaaaaa cagtttatga agtaatgaat
     4381 cctttcatac ttgagcctgg caaaagttgt tcattgccca tcacagtacc tacaacgatt
     4441 catggtgctc atacggttgc tgtttcgcta tctgtttata aaaatgatga gctttatgga
     4501 cagcaacagc tctttcttaa aaaagaaaaa atgagattgc caagaactgc tcatcataat
     4561 gatgcaccac aaatttcagt aaaaggcaca gagctgataa tcaaaggaga atcctttatt
     4621 tatatatatc aattagcaaa agctgcgttc acttctgtaa agatcaatca aaaggaactt
     4681 ttggaacgtc ctactcaatt ttcaatttgg cgtgcaccaa cggataatga tcgtaactta
     4741 gtaaacaaat ggaaagcagc tggatatgct gaaatgtctt tagaaatcag aaaaaaagac
     4801 tggaaattcg tagatgatca tctcctgatc gaaacgtctt atcgaggagt tattccagga
     4861 ttacagccaa tttttacaat gcacatacag ttgaaaataa atggtgcagg cgtaatcaac
     4921 caaaaagtat cagtggagaa aaatccgatt tttccagatt tccctcgctt tgggatggac
     4981 tggccgttga atgatacttt ttcgaaggtt tcttattgtg gctatgggcc gtatgaaagt
     5041 tatcctgata agcactatgc tgcgatgttt ggtatttatg agatggagat tgaggaattt
     5101 gatcagccat atatcaaacc acaagaatat ggtaaccgct ggcaatgtga ttgggtatca
     5161 atcaaaaatc atcaaggatc taccatgacg gtttatggaa actctaattt ttctgctctg
     5221 ccttatagtc aagctcaatt aaccgaaaag tcacaccatt atgaattaat aaaagaaaat
     5281 agaacttggt tcaatttaag ttataaacaa aatggtatcg gctcacacag ttgcggtcct
     5341 tatttagaag atcgtttccg ttttatggaa gatacttttg actggaatat agtgattact
     5401 ttcaaaggag aaaatagatg aaagaaacaa agatcaatca agtcaaaaaa aaattatctc
     5461 gttatcaaga agttgtttat cgaaaccatt gcttaaaatg gtcaaaaaat aagctgaatg
     5521 ttcgttttaa tggattattt tatcgtttcg aacagttaga agaagtctgg aaaccggtaa
     5581 cgaatttcca atcatgtcct gtacagctca taaatgatcc aaaagaaaca tacgatttat
     5641 gtataacaca cctttataac cagcatttga aaaatgacgg attttattta aaggttacta
     5701 atgacaatca acttacgatt gaatcagcaa accttcgagg gtttcaatat gctatggaag
     5761 ccttattaga gagttttttt tcggtaggag atcaattatt attaccggaa ttgattctga
     5821 aacatgaacc atcagtgaaa attcgaggaa tcattgaagg gttttatggt atcccctgga
     5881 cacatgaaat gcgcctctct cttttttcat atatgaaaga taaccaattg aatactttta
     5941 tgtatgcccc taaagatgat gaattattac gtaaaaaatg gcgtgaactc tacgatgcgc
     6001 aagaattaga caaatttaag gagcttttat ctgctgctga ggctagcaat atcgattttt
     6061 ggtacttaat tagtcctgga aatgatattg atattacgtg tgaagaagac attcaagttc
     6121 ttctgaaaaa gttagaacaa ctgattgagc ttggcgtatt tcaatttggt ctattaatgg
     6181 atgatattga ctatcaatta aaaggtgctg ctaaacgccg ttttcgagag ccagcattcg
     6241 ctcatgccta tttagtgaat cgagtagagg aatatcttag cactcggttg tccaatcatg
     6301 aattagtcat ttgtcctaca gaatatgata atcgtcatgg ttctcgctat ttagcaacgt
     6361 taagtcaaga aataccagaa caattaccgt tcttttggac tggcccaagc acgttagcgg
     6421 cgtcaatttc tacagaagag ctgcaggaaa tggcttctgt ctatcaacgg ccgatgctga
     6481 tttgggataa tattccggta aatgattatt tagaggataa agaactattg ttcatgagtc
     6541 catatgagaa tcgaacaccg aatctttcta aggagagata ccaggttaca ggagtagttt
     6601 cgaatccaat ggctcaatta gaagcttcaa agtttacgat caatagtatg gctaattact
     6661 tatggaactg tgagcgtttt gatcctttgg aaacttggac gtcagtcgtt gaaaaagtag
     6721 ttggaagtaa actacaacca gcttatctta ccttaaccaa tgcttttcca aatcattaca
     6781 caagcagtct gctttcagat gaagaattac tgctagccaa agacccagaa tgggtcacaa
     6841 aagaaatggc tgctttagtc caagcagtaa aatggatcca gcaagaacaa catacatcta
     6901 gattgagaac agaattggag ccttggttac aagcaatcac agaaagttgg actttctggc
     6961 aagaatggca attaaaaaaa gatccaaaag aagcagaaga atggttggct aagaaaaaaa
     7021 cacaccgcat aggacgtgat ttagtaactg cccatttaga gcaaattatc aaaagttaga
     7081 tatcttaaaa aataaaaagc aagaaaaagg agaacaaaat ggtcaagaga ttaattagtg
     7141 caaactacag tgatgtcaag aaaatgacgt caaaagagtt gaagcaatcc atcaaagcga
     7201 gtgaaggacg ggtgattgtt tgtgaaacgg ttgctaatac tcttccacaa tctggagaaa
     7261 ttacgaatgc agaagttgcg tcagcatttg gtgcagattt gattctttta aatggtttcg
     7321 attgttatcg tcctgcagtt tttggactac caaatgtgac gattgagaag gtttttgatc
     7381 tacagcaaac gattgaaaac ccgattccta aattaaaaga actagtcggt cgtcctgtgg
     7441 gaattaattt agaacccatt gcccctaaaa caaatatgat gagtgagaaa gtagaaatca
     7501 gccaaggaag agcaagctct gttgaaacga tacaaaaagc agaagaacta ggagtcgatt
     7561 ttatttgctt taccggtaat ccaggcactg gagtaaccaa tttagaaatt actaaatccg
     7621 taaaaatggc aaaaaagtat ttttcgggat tgattattgc tgggaaaatg catagcgccg
     7681 gctcagaaga gccagttgtg aataaagagg cgattatttc ttatgcggaa gcaggcgcag
     7741 atattttact agttccttca gtagggtcta tcccaggctt tttagagcaa gacctaattg
     7801 aagcggttcg gttggcaaaa aaatatgatt tgttaacgat gtctgcaatc ggtactagtc
     7861 aggaaagtgc ccgaccagaa acattgcggc aattagccat tatgaataaa agttgtggtg
     7921 ttgatttaca acatattgga gatgctggat acgggggatt ggcaccagca gaaaatattt
     7981 tcgaaacctc ggttgcaatt agaggaatgc gacataccat caactgcgtt a
//
LOCUS       DACESX010000116         8010 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.116, whole genome shotgun sequence.
ACCESSION   DACESX010000116 DACESX010000000
VERSION     DACESX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.116"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            140..1231
                     /gene="phnW"
                     /locus_tag="IXF88_002205"
     CDS             140..1231
                     /gene="phnW"
                     /locus_tag="IXF88_002205"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1508919.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1224..2009
                     /locus_tag="IXF88_002206"
     CDS             1224..2009
                     /locus_tag="IXF88_002206"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1508920.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(2355..4046)
                     /locus_tag="IXF88_002207"
     CDS             complement(2355..4046)
                     /locus_tag="IXF88_002207"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1508921.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(4468..5415)
                     /gene="arcC"
                     /locus_tag="IXF88_002208"
     CDS             complement(4468..5415)
                     /gene="arcC"
                     /locus_tag="IXF88_002208"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1508922.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(5530..6549)
                     /gene="argF"
                     /locus_tag="IXF88_002209"
     CDS             complement(5530..6549)
                     /gene="argF"
                     /locus_tag="IXF88_002209"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1508923.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(6640..7869)
                     /gene="arcA"
                     /locus_tag="IXF88_002210"
     CDS             complement(6640..7869)
                     /gene="arcA"
                     /locus_tag="IXF88_002210"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1508924.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2288 a         1636 c         1461 g         2625 t
ORIGIN      
        1 ctttacaaaa atacatcatt ttttattcaa ttttttcaac accttgattg aatcacaata
       61 atcatcattt atattggatg ttaacaagag cagcttcttg ctctttgtgt atttcaaaaa
      121 agaaatgaaa ggaaatcata tgtataaatt attaactcct ggaccattga cgacttctga
      181 aacagtgaaa aatgctatgc tgatcgatca ttgtacttgg gatcaagaat acaaagaaat
      241 tacacaatgg ataagaaaaa aattattgga tcttgctcaa gtatcggaag gaatatacac
      301 aacggtctta atgcaaggta gcggctcctt tggggtagaa tcggtattaa gttctgtgat
      361 caggccagaa gatactgtac tgatttgttc aaacggcgca tatggacaac gaatgatcaa
      421 catgtgcgaa tgtctgaaac tgaactatac cgtttatgag gtatcggaac atcaaatcct
      481 ccaagctcat gtgattacac agctgattga agagaatccc tctatctccg cactagcaat
      541 ggtccatagc gagactactt ccggtatttt gaataatttg gaagagattg ctgctgtcac
      601 acaagaaaaa gatcttttgt ttatagtgga cgcgatgtca agctttggtg ggataccgat
      661 tcgtgtagcc gaactaggga ttgatttttt gatttccagc tcaaacaaat gtattcaagg
      721 tgttccaggc ttttcttttg tacttgctaa taaagcggaa ctagaaaagc gaaaaaatgt
      781 gagtcgaagt ttgtcacttg atctatatga ccagtaccgc gtgatggaaa acgagaatgg
      841 caaatggcgt ttcacttccc ctacccatac ggttttggct tttcaacaag cgctgaagga
      901 actagaggca gaaggaggaa tcgaaactcg atattcacgt tatctcgcca ataatcatgt
      961 actacgacaa aaaatgacag agatggggtt ccagcctttc attcaagaat atcaggggcc
     1021 attcatcacc tcttttcttt accctaagga ctggcgattt gattttaatc atttttatca
     1081 gtatttaaag caaaacggat ttgtgatcta tcccgggaaa atcagcgatc atgaatgttt
     1141 tcgtatcgga aatattggcg agatctatcc ggaagatatt ttggaactga cagcaattat
     1201 caaagactat aaggagaacg ttaatgatta aaactgtaat ttttgattgg gcaggaacaa
     1261 ctgtggactt tggctgtatg gctcctgtcc acgcttttag aaatgctttt ctagaaaaag
     1321 gaatccaact gactgacaaa gaaatccgcg aaccgatggg aaaattaaaa tgggatcata
     1381 tccaacagct tctttccctc ccttctgtca aaaaacaatg gatagctatt tatggacaac
     1441 tacctcagga aaaagaggtg gaggaattgt atcaacaatt tgaacattat ctgtttaaag
     1501 atttggtcaa gcattctacc ttgaaacccg acacattgga aacgatcgaa cggttgagaa
     1561 acgctgggat caatattggt tccacgactg gatttactgc tcctatgatg acaatcgtag
     1621 cagaaaccgc aaaaaaagaa gggtattcac cagacttcat ggcaacttct gaagacgtcg
     1681 aagggtatgg tcgaccgttt ccttatatga tccataaaaa catgcagtac ttcaaaaaca
     1741 agtcggttga tgaagtaatc aaagtcggag atacggtctc agatatccag gaagggaaaa
     1801 atgccggagt gttgacagta ggtgtaattg aaggtagttc gctaatgggg atttcttatg
     1861 atgagtacca aaaattagag atggggaaaa aagatcgttt aaaacagtct gttgctctta
     1921 gattccaaca agctggcgct gatatttgtg taaacaattt aaaagagtta acagaataca
     1981 ttttgtacgc tgacaagaat ccagaatgac ttttatacaa aatcagaggt cgtgaaaaag
     2041 ctgtactgca tcaagtaata agaacagcca aacaaaaata gctcctcata tttttcgttt
     2101 ggttaggctt attaccgcag atgcaagctt ttgaacaccg tttattcgaa aaaaaaaggg
     2161 agctgtgaca cgacttttgt cacagtcccc ttatttacaa acaaacagtg tttaagagac
     2221 aacctctcca ctaaacggtg ttcattcagc tgactctcgg tatttgctca aaaaacgata
     2281 aaatttgaaa agctattttc tcttttgttt ctctaatact cggatcagaa cgctgatttc
     2341 acattctctt tttcttacat cttatctggt gcatgaagtc ctagcaaacg aaggtcttct
     2401 ttcaataata cagtcactgc ataaactaga gctaagcgtg cttctttttg ttcatcatca
     2461 gctaaaatct tcgtatgagc atagtatttg ttgaatgctt gtgctaattt gattgcatgt
     2521 ttcgcaatca ctgacggttc gtatttttcc ccagcagaca agaccgtttc agggtatttt
     2581 tgaactaatt taaccacttc ccagctggta tcgtcattca atgcatagtc tgctgtttct
     2641 gaagggacaa atcctgcttt ttccaacagg ctgactgcac gagcatgtgt atattgcaca
     2701 taaggtccag tttctccttc aaatcgaaca acttcttcca ggttaaagtc aaatgtattc
     2761 agtcgatcgt ttttcaaatc gtggaaaatg actgccccaa ccccaacttg tttagctaca
     2821 gcatctttgt tttccaaatc agggtttttc tcgctgattt gttctttagc agattggatt
     2881 gcttcattta atacttcttc taataagacg atctttcctt tacgagttga taatttttta
     2941 ccaccttgcg tgatcaaacc aaatggaata tggcgcatat cgtcagacca gtcaaagccc
     3001 atttccttca agacagcttt caactgttta aaatgatagc tttgttcatt ccctactaca
     3061 tatagagatt gtttgaagtc gtaagtacgt ttacggtaca atgcagcagc taagtcacgt
     3121 gtaatgtata gtgtcgcacc gtctgacttc ttaatcaacg ctggattcag gtcataagca
     3181 gataggtcaa cgatctctgc acctttatcc tcgttcagca aatgtttttc ttctaaaagt
     3241 ttgacgattt catccatctt gtcattgtag aaagcttcac cgttcaacga atcgaaacgt
     3301 acttccagca gatcatagat tttattgaat tctttcatcg attcatcacg gaaccattgc
     3361 cataattgaa tagcctcttc atcgccttct tccagacgtt tgaaccaagc acgagcttct
     3421 tcatttaatt ctggttctgt ttcagctact tcatggaatt ggacatataa acgaagcaat
     3481 tcattgattg gttctgcttt aacagcttct tctgtacccc attttttgta agcgacgatc
     3541 agttttccaa actgtgttcc ccagtcacct aaatggttga tgcggattgg ctgataaccg
     3601 attttttcca taatgaaacc gatcgaatta ccgataactg tcgaacgcaa atgtcccatt
     3661 gagattggtt tggcaatatt aggagaagac atgtcgattg gtactgttcc ttgattcccg
     3721 atattgctat ccccataatg ttctttttct ttcactactg tttgcaaaac ttttttgctg
     3781 ataagttctt tattcatgaa aaagttcaag taaggtccga caacttcgat tttttcaaag
     3841 ttggcagaat cgattttttc tgctaaatct gcagcgattt gctgtggcgc ttttcggtaa
     3901 acttttgcta atgaaaaggc tggaaatgca acatctccgt gttcagctga ttttggattt
     3961 tctagtaatt gttcgacttg ttccagtgtt aaatcatctt tgacagcgtc atagacagct
     4021 tttgcgacaa gatctttatt attcatcttt attcctccta aagtgctccc ttgattgtta
     4081 aacaaaaaaa atctctagcg acggcatact tagtatgcca tcactagaga cgagatttcc
     4141 cgcggtacca ctccaattgt cctaaaaaag gaccagctcc atctgatatc ggtagacgac
     4201 cgtctgttta aaacagaatt ctccaagtgc gcttcgttgt atcttcttca ttcggcttcc
     4261 accacttccg aattcgctag ccaagaatga tacaagtact ctcttgttca acaaatctta
     4321 gtcttattaa actttcttga attatatcag aaaaaaaaac gaaaggctag tcgtttctga
     4381 taaaaaaaca atccagatgg cctaaaggag aagaaatacc acctggatag ctataaatca
     4441 tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc cttcttcaga
     4501 agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt ttggttttgc
     4561 ttcaacaaat gcaatagctg cctctacttt cggaagcatg cttcctggag caaattgctt
     4621 ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct tttgatctgg
     4681 tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca aatccgcatc
     4741 gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt ctcgtccttc
     4801 taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga ctgtgataac
     4861 tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg gtttagggga
     4921 agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag tggagtcagc
     4981 attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt tacttggatt
     5041 tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa ccacatcttt
     5101 atcgatccct ttttccttca gtacttcgcc catggcattt tgtaaccagt aaccgataga
     5161 tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct cagaatcagc
     5221 agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg agatgatcaa
     5281 ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta acgcttcttg
     5341 ttgggctttt gcacttgcat ccgtagacaa gattgcatta ccccctaaag cgacaactac
     5401 ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc tagataacaa
     5461 aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt gagatgaatg
     5521 tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca tgatcgcttt
     5581 gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc tgcggaagac
     5641 ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt caccgtaaac
     5701 agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat ctgtgttgtg
     5761 tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga tacgttcttc
     5821 gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt ctgctccgtc
     5881 tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt cagcaaagcc
     5941 ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa cgatacgcat
     6001 attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt ttcgaccatc
     6061 tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga tagttaaaaa
     6121 gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa ctggaacacc
     6181 agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt cgattccatc
     6241 aaacatacga cctagtactt tagctgtatc ttctgtggat tcttttttac ctaattggat
     6301 atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag tagtaaatgc
     6361 agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc cttctagata
     6421 gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat cgatcaaata
     6481 ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt ggaatactga
     6541 ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa gaggaaatcc
     6601 cgacggcagc tgacagtaag accgccgtca ggaaacaaat tacttcaagt cttcacggac
     6661 aagtggttgg ctcatgcagc gtggaccacc acggcctcgt gacaattcac ttgaattgat
     6721 ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat ttcggttata
     6781 agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc attgttctcg
     6841 aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat ctaatccaag
     6901 agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat catcaccatc
     6961 tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg gatggatagt
     7021 gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg cacggttgtt
     7081 tggaattttg attgctaaca ctttttcaaa tcctgaattt tttgcgaata ggtggcgtgc
     7141 taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta ctactttatc
     7201 gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat ccaaccatac
     7261 ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg taaacatcga
     7321 ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc ccattgatgc
     7381 ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg gatagtcatc
     7441 gtctgctgac agatcgtata gactgctaga acgtacttca atatcttttg tacgaacccc
     7501 agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact catgaagacc
     7561 ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca agaattgttc
     7621 tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa gagtctcgac
     7681 gccttcattt tgcaatgtct ttgcaaagtt gtcatgttct tcttgagcaa tcggaagata
     7741 tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt ccacttcttg
     7801 tccaggcctt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa cgtgaatagg
     7861 tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat cctccttttg
     7921 taatcacttt cataattttg tctcattcaa aatgagacat tcctcacatt aatattcgat
     7981 aattaaataa ttaaacgttt ttctattttt
//
LOCUS       DACESX010000117         7932 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.117, whole genome shotgun sequence.
ACCESSION   DACESX010000117 DACESX010000000
VERSION     DACESX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.117"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..2008)
                     /locus_tag="IXF88_002211"
     CDS             complement(<1..2008)
                     /locus_tag="IXF88_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1508925.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
     gene            complement(2024..3020)
                     /locus_tag="IXF88_002212"
                     /pseudo
     CDS             complement(2024..3020)
                     /locus_tag="IXF88_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3218..4459
                     /gene="sstT"
                     /locus_tag="IXF88_002213"
     CDS             3218..4459
                     /gene="sstT"
                     /locus_tag="IXF88_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ1508926.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4606..5241
                     /locus_tag="IXF88_002214"
     CDS             4606..5241
                     /locus_tag="IXF88_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1508927.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5432..6172
                     /gene="truA"
                     /locus_tag="IXF88_002215"
     CDS             5432..6172
                     /gene="truA"
                     /locus_tag="IXF88_002215"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1508928.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6317..7915)
                     /locus_tag="IXF88_002216"
     CDS             complement(6317..7915)
                     /locus_tag="IXF88_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1508929.1"
                     /translation="MLFLSFAYVIPLFMSMGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2625 a         1234 c         1489 g         2584 t
ORIGIN      
        1 ttttttgtgt cgcccatgtt gcactgtact ggttacctgc aacaagatca tattgtatca
       61 gacgataatc ttctagaatt tctttttgtt tctcagttaa atttttttct tggataattt
      121 gactatcctc tgtaacaaag acctgttttc cattaaaacg attagacaag ctttctgtag
      181 gatcaattgt taatgcaggt agatgattag atacttctgt caacaacgca tagtaaggag
      241 ttactttgat attcgcatgc tcaagagcca tagcaccaaa gttattagga gcaacaactt
      301 ctttatttaa cgtagctgct tgttcacgac tatacttgtt gttataaatg aaataatctg
      361 tctggtgctg ttctaatcca tattttttcg aatcattccc actatagatt gcgggtaaat
      421 ggtcaccata aaaaacaaag gtaatcggtt tatcgatttt gtcgatttct tggatgaatt
      481 tttttactgc tgtatcggta tagtgaaggc cttgcatata agtttgtaat tcttttcttc
      541 tattaggaat gacagcagtt ccttcagcag tgtagttcaa ttcactatag taatcatcat
      601 aaggcatatg gttttgcata gtggataatt ggatgaattg tgacgtttct gtattgcttt
      661 gtaaagcttt caaggtttct ttgtatgctg aatcatccga tatgcgtggg ctttgttcta
      721 ttttatccgt ataagttaat ttatccggac tgtctaaata atagaacctg tcaaaaccaa
      781 atttatcaaa cacttgtttt ctgcgataaa gagcagctgt aaaaggatgg atggctattt
      841 tttcatcgaa tagatcggta atatttggac ttattttctg acgctccact agctgggtat
      901 aaggtgtaac taacgaagca gacaggttac tgatactcaa gcctgtcagt ccttcccatt
      961 ccatgttagc tgttccgcca ccataaccga cggataacat caatcctcct gttccttctg
     1021 ttataatgct tttgatgtaa ggaatagggt ctgtaggtac tgtcagatta gggacacgtg
     1081 caggatcact gaagctttca cttaaattga gaacgatcgt ctgattgttt aaccagtcac
     1141 ttcttgtttg gttgatttca tctgcctttg tgttgtactt tttcattatt ccattaattt
     1201 tttctttcga atagccagca ggttcttcca tgattttaat gtcgatattg tttaggaatt
     1261 gaatgactgg tccgttatct tttacggcgc cttgctggtt aaagaaagtc ttgtttactt
     1321 taaataaatt aaacagcaag aaagaaggcg aatttttgtg attaatgaaa aagacacccg
     1381 aaaaacatcc taataataca gcgatactga taaatcgacg tttccaatga atcttcaagg
     1441 aatactgttt ttgtagtcta cgttgtacaa taatactagt aatcaataat aggagaagga
     1501 tcaatccgcc cacgattaat aagactgggc taaccatcga taatagttct gacaatccgg
     1561 tcagtatttt taaatctgaa ggaaagacag gctcttcccg catttccatt ttgattactg
     1621 tcgaaatagt gagcagtaag ttaatgatca aggtagcagt caaggcgtac caaaaacgat
     1681 tggaaatcaa caatagtaag aagaaaaatc ccataataat gatgattgtc aggatcatgg
     1741 gtgcttgtcg ctgagtaaat atttttaagg caacctggat agtttctttc caaccttctt
     1801 ttggttctaa aacgaaaatc tgcacgaagg taaagcagta aaaaaatgct gcaactaaaa
     1861 aataactttt atgaacaaaa agccaatgaa ttcttttttt ccaacgctga gttaattgtt
     1921 caatcgaatc aaaagcgaag tattcgatga aacgatgaat gaaacggatt ttttgtatat
     1981 aaaataaaaa gataactagg agggacactg ctaataaata gcaaataaaa aactgcccga
     2041 tatttatcag ccatgccttc cctgagtttg gaaattcacg tttcataaat gtagatacgc
     2101 gataagaaac taaagggaaa ttacatataa cttgagtgac aatcaaaaat acagcaagaa
     2161 aatttcctct tccttttata cctaatttat cagatagcat ttctaaaaat aaaaacgaag
     2221 agaaagtata atacattgcg aagagagaat atggtttaga aaacctcagc gctgtatctg
     2281 tatttcctct gatgatgagt gaaatttgtg tcatcaaaat aatcaaaccg aataaaacaa
     2341 tcaagctgat aaatacttga ggaagcttat agcggacctt ttctttccag ttccattctt
     2401 tcaaaccata acccattaaa aataaataaa aaatccataa aatactctgt ccattctgta
     2461 atgcaaacgg gtctttatta aatagagtag agcagccaac taatccaatt gttaaaaata
     2521 atagtagata tttaattgtt gttttcgata atttatttaa aaatccaact gtataaggaa
     2581 tactacaaaa aagcaaagta caagaaacag cataagtaaa gtaattctga ctgataggtg
     2641 ttaggataag ccaataatca cgaatattca agctgccagc ttgtacgtaa aaaaaagata
     2701 ttgcaaacta gtgtggctat taggaatata aaggcaaaac gacttaaata atgtaaaact
     2761 tttaaacggg cgattttttg atattggtaa cccatgtaaa gtgggatcaa atttgcacca
     2821 ataacaacgg aagtttgtaa aaatgtatag atgtagaatc gaccagggtt tgttgcatat
     2881 gtaggaatag tgactgctga aatcaatata cgctgtaaaa ccatcattat acaagcgaca
     2941 aagattgtca ggaagccata actctccttc tttatttttt tcaagaataa acacctcaca
     3001 aaaaaattgg acaaaaacat aagaaaacgt ttcctgataa gttgcccatt aataacatcc
     3061 taacttagaa tatctcaaaa aacaacgtgt agacagaaaa agtacaaaat tagcaagaaa
     3121 tggttgtttg tattgttaga atttttagaa aattatacct ttagtttctt tgcataaaat
     3181 gatacaatga ttgttaaaat tgtgaaaggg tggtcatttg ttagagaagt ttttggatat
     3241 gtcgttagtt caacgtattg ttgttggtat tttagtcgga atattgtttg gtatcttttt
     3301 ccccacatgg acatttattt caatattggg aacgttgttt gtcagttgct tgaaggcaat
     3361 cgcacctatt ctggtttttt tcttgacgat ggcgtcaatt gccaagcata aaattgggaa
     3421 taagacattc gtgaagccta ttttgatctt atatttagtg ggaacattgc tttctgcact
     3481 tgtagcagtt attgtaagtt caatttttac tgttcctatt actttacaag aaacagtatc
     3541 tgaaaaagca ccgcaaagtt tggaaagtgt tttatctact atgttgacga atgtcacaca
     3601 aaatcctgtt caatctctta ttgatgcgaa ttaccttggc gtcttattct gggctgtatt
     3661 gttgggacta gcttttcgag cacgttcaga atcgacaaaa gagttggtgg atcagatatc
     3721 tatagcgttg tcacaagtcg tacaagtgat tattgcattt gctccgatag ggattttagg
     3781 attggtttat caatcaattg ctacgactgg aatagctgga ttggcagagt atctgcagct
     3841 tttacttgtt ttgatcggta cgatgctgtt tgtcgcatta gttgtgtacc cattcctcac
     3901 gtttttgttt attaaagaaa atccctatcc attgattttc ttttgtttga aagaaagcgc
     3961 acttcccgct ttctttaccc gaagttctgc cgcaaatatc ccgataaaca tgatgttggc
     4021 tgaaagattg aaattgacaa aagagtctta ttccatatcg atcccgttag gtgctacgat
     4081 caatatgggc ggtgccgcga ttaccatttc cttgatgacg ttgactgctg tacattcttt
     4141 agggattgaa gcaccttttg ttttgaaagt ccttttatgt attttgtctg ctttagctgc
     4201 ttgtggagct tcaggtgttg cgggcggttc actgttattg attccattag catgtagttt
     4261 gtttggtatc tctaatgatg tcgcgatgca aatcgtcggt attggattta ttatcggtgt
     4321 tatccaggat tcaatggaaa ctgccttaaa ttcgtcaagt gatctgttgt ttactgcaat
     4381 aggagagcta ggcgcgcgta aaagaagtgg ggaaaaaatc aaattgaaag attgtttaga
     4441 tggattaagt gaacgttgaa cgttagatat ttagtatttt taaaatgatt cgagttttta
     4501 atgagttata tagatgttcg tgtaaataga ggtttctgtt aaatcaagat aaagtcgctt
     4561 ataataacaa ttaaaagcat aggtaattgt tattggaggc gtatcatgga ttttttacgt
     4621 aaatttggtt ttttcttttt attattaggc gtgctgagtg attttttgac tccgtacgta
     4681 ttaggattgt attatcctga attagatcaa atgaagagcg tgatcagtgt atttggagag
     4741 gtttccagtc ctgtaagaaa agcgtttctt atttggtctg tggtttcagg gatctttttc
     4801 atcctgtcat tgcctgcatt gtatgctgca tttgtaagta catctcgtgt gttagctacc
     4861 ctagttacag cagccatcgc gttctatggt ataggtgatt gtatctttac aggtttgttc
     4921 agtatcaata cccatgaatc ttcgtggaat gtctcgacat gggtccataa tatcggatca
     4981 ggtttaggtt acagtggttt tttattgttt ccagttcttc ttttcttgat ctatcgaaaa
     5041 aatgggcaaa tggacgtaag ccacttatac cttggactgt ctgtgatcag tttgcttttt
     5101 gcggcgattt atggactggc aagaattcct tctataaatt cttggccatt actgaataaa
     5161 ataggttttt gccaaagaat cagttttttc tttaattatg tgcccattgc ctggttgagt
     5221 atcttgcatc tcaaacagta aaacttgagc cgtcaaattg aacagtcagc ggatacttca
     5281 tccggtggct gttttttgtt tttctgaaat atatatttcc acagttttct tgtatagctt
     5341 ctgttttaga gaagaaagga gctatatttt gtgctacaat cttaaaaagg tagagagaag
     5401 accaaatgga agacagagga gccactaaaa tatgagaaac atcaagttga caatcgaata
     5461 tgacggaaaa agatattcag gatggcaaag acttggagat gacgataaaa ccattcaagg
     5521 aaaaatcgaa aaagtattgc atcagatgac aaatgaagag atagaaatta ttggatcagg
     5581 aagaacagat gctggcaccc atgcgcgagg acaagtagca aattttaaaa caaacacaga
     5641 aattggactg gcagaaatga tcgactttat gaatcgttac cttccgcggg atattgtcat
     5701 caaaagaatc gaagaaatgc ctgagcgctt ccattcaaga tataatgcgg tgggcaaaaa
     5761 atacagctat tatgtttgga acaacgtgat tccttcagca tttgagcgaa accacagctt
     5821 ttatttccct caagagttgg atatggacaa gctgaatgca gcttgcgaaa aattagtggg
     5881 tacacatgat ttcattggtt tttctgcatt gaaaaaatca aaaaaatcca cagtgcgtac
     5941 gattgatgaa ttatctatcg aacgaaaggg agaaatgctt cattttactt ttgtcggaga
     6001 cggattctta tataaaatgg tgcggattat catggggaca ttactagaaa tcggcacagg
     6061 tgccatgccg atagaaacaa tcgatgaaat atttgaaagc aaagtgcggc aacatgcagg
     6121 agaaaccgtc cctgcacaag gtctgttttt ggatgaagtt tattatcgat aaaaatactt
     6181 tgataatgga tgaaacgcat gtttcacagt ataaatatcg atgttaaata atgtatgaag
     6241 caaattaaag aaatataaaa aaagactttt tagcctgttt atgtgtacag ctaaaaagtc
     6301 tttcttaaat aaaaatctag tgagaatgtt gtacttctct tctcggttgt ttttgtttag
     6361 aaaaagtaaa tcgataaagt aaaaataaaa tcaaagtaac gatagataaa tatcttgaag
     6421 caattgctag ttttgtgtac tgataagtaa taacgacttt atttatacct ggaggaatag
     6481 ttaattcagt tgtgccaaac ttagataatt ttgtttgtac tagtttgcca ttaatagaag
     6541 ccacttgccc tttataacga taaataggtg tcgtcaagac agtattttta ttattcgaat
     6601 tatctaattc aattgtaagt ttgttactca taaaatgagt tgttggattt atttcctggt
     6661 tacctagtaa atattgatca ttattaatca tatcaggatg atagattgac tctttgtttg
     6721 cataatcagt atgattgtaa ttttcagcta tagcaacagc gtcttcaggt gctataagtg
     6781 ttaatgaatc tttgttagca tggatagaat tgcttactcc tgagagatgc aatattagtg
     6841 caagaaacgt aaagaccatt attttccatg aattattttt agtagaaaaa atataataga
     6901 tcactatact catcgtatag gctataaata aagaagaaaa accatttaac cgccagacaa
     6961 attgtagact cttaactggt gtatggttaa aaagttgcca aggaaataaa ttggttgaac
     7021 aaaaaagaac aaatacacca agaaagaaaa taaataaatc atcagcagtt aattttttta
     7081 taaaaataaa agcaccaatc aatccgagaa ataagaataa tccaatagta tagttgttat
     7141 agttattatt taaaatgtgt gtcagcatat cactaggagc cataccactt aattcttttc
     7201 ccagtgggac tttcaattct tgtgctctag attgttgaat aaaaggaatt aagaatcccg
     7261 cacttaaaaa aaaggtgagg acggtagctt ttaaaagact taagagtcta gcgatttttt
     7321 ggtcccaaaa ataaaaagaa agaaacaacg ttcctccaat aaatacagaa accatagcga
     7381 cagaaagtaa atgagtatac actactagcg tcatgccaat agacaaccag taccattttt
     7441 ggtaatctct aatataaatt tcgtaacacc ccattagtat caaaggcaaa aatgttaaag
     7501 cgacagcttc tcccaatgaa gcacgatgga atatgtcaat ggcacgatag gcggaaaatg
     7561 tataaataat cgaaaataac agcgaaatat aacgattgtt ttttatttgt ttcatagaaa
     7621 aataagaaac gaccattgtt aaaaaagtga taaaaaaata gtagatatta taacttaaaa
     7681 caagatttcc catcatttta taaaataaaa aagctggata aagagtaagc catggataaa
     7741 aaatattcat cattgttcca tgatgatcga agttattgaa gtttactgga gaactccata
     7801 cattatctag tcctatgatt cgagataaat gaaagtaagt atcctgattg acactatgat
     7861 agattcccat agacatgaat agagggatga cgtaagcaaa acttagaaat aacataaaaa
     7921 tgatatctat ta
//
LOCUS       DACESX010000118         7892 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.118, whole genome shotgun sequence.
ACCESSION   DACESX010000118 DACESX010000000
VERSION     DACESX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.118"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(86..2257)
                     /gene="copB"
                     /locus_tag="IXF88_002217"
     CDS             complement(86..2257)
                     /gene="copB"
                     /locus_tag="IXF88_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1508930.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2277..4463)
                     /locus_tag="IXF88_002218"
     CDS             complement(2277..4463)
                     /locus_tag="IXF88_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1508931.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4463..4672)
                     /gene="copZ"
                     /locus_tag="IXF88_002219"
     CDS             complement(4463..4672)
                     /gene="copZ"
                     /locus_tag="IXF88_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1508932.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4685..5125)
                     /locus_tag="IXF88_002220"
     CDS             complement(4685..5125)
                     /locus_tag="IXF88_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1508933.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5199..5738)
                     /locus_tag="IXF88_002221"
     CDS             complement(5199..5738)
                     /locus_tag="IXF88_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ1508934.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6353..7819)
                     /locus_tag="IXF88_002222"
     CDS             complement(6353..7819)
                     /locus_tag="IXF88_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1508935.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2145 a         1744 c         1382 g         2621 t
ORIGIN      
        1 gagccagaat gaggttcatt ctgacccaga tttgattttg tacaacccat cagtccttat
       61 tgacaaagag cccctttttt cattattatt tgattttcaa tgtcaaagca tttagagcta
      121 caactaccgt acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa
      181 ttggcgcaag aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc
      241 agaggttctg gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat
      301 cttttggatc gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg
      361 caccgattgc catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac
      421 ccaccatgat tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat
      481 ctggtaataa accgccataa tattcttcga tccctaaata atctgctact gcttgggcag
      541 cgtttttatt gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga
      601 taaattcttt tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat
      661 tttctaaaat caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt
      721 gttctacgtt taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt
      781 ttttgactgt tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag
      841 gcttgatttc atgttctttc aaataattca taatgccaac agccaatgga tgattggcat
      901 tcgcttccaa ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta
      961 cttcgacacc cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga
     1021 tcacatctag atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt
     1081 tagctgctat tgatgtggag cgagcaacaa ctaatgggat agctaaacct agagcatgag
     1141 gacaggcgat gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga
     1201 acaaccaggc gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg
     1261 ccactttgtc agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt
     1321 ccataacctt cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc
     1381 catccccatt gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg
     1441 attcgccagt cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg
     1501 ttggcatttt atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg
     1561 aaactgtctc ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg
     1621 ctagtttttg taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta
     1681 gtagcataat aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg
     1741 gattgagcag atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca
     1801 tcgcgatcag tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta
     1861 aaaatggctg accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag
     1921 aaccaggaaa cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga
     1981 ttgggatagc taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg
     2041 aatgatccat gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt
     2101 ttgctatatc tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat
     2161 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     2221 tatgctcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     2281 tttttcatgt gtcgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc
     2341 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     2401 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac
     2461 aactcgattg tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt
     2521 tccatcgcaa tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc
     2581 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     2641 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     2701 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     2761 tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca
     2821 gccacggcaa tccacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     2881 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     2941 aatcgtttac gtgtacccgc aaaatagcgt actccattaa tcgtaccgct gatcccagca
     3001 cctgggtggg ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     3061 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     3121 aggacttctt ttgaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca
     3181 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
     3241 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggggtcgct
     3301 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
     3361 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
     3421 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
     3481 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg
     3541 acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga
     3601 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
     3661 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
     3721 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
     3781 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
     3841 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
     3901 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
     3961 ccaattgcta ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct
     4021 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
     4081 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
     4141 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
     4201 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
     4261 taaccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
     4321 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
     4381 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
     4441 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
     4501 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
     4561 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
     4621 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
     4681 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
     4741 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
     4801 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
     4861 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga
     4921 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacgt cccagcaaag
     4981 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
     5041 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct
     5101 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
     5161 agcttatgaa tacagattac gcttgtaaac aaaaactgtc aattattttt gctctctatt
     5221 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
     5281 cttgcattag ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc
     5341 cagataaaag ataaaaattc taagtattcc ctgtttagtt tctctgaaaa tgattcaggc
     5401 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
     5461 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
     5521 tctaatgtag aagtataatt tccttcaaca atccatgacg gattagatac cataaacgat
     5581 tgctgttctt gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa
     5641 aggtctagcg gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat
     5701 tttcccgacc cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg
     5761 gaatttggtt cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag
     5821 tcgtgtcaaa gtcccttgtt tcgaataaat ggtgttcaat cagctgacct tcagcattaa
     5881 ctcaaaaaac ggcaaaatat gaggagctat tttttcgttt gttcctctaa tactcaggtc
     5941 aaatcgctga ttttacaacc tcttccttaa acggtgttct cctgccaaca cctcggtcac
     6001 aaggcacaat agtcaacaaa catgagaagt tgttttttga aattgctcct tgtgactcgg
     6061 agttaaacgg caggctcact acctctttct taaacggtgt tcaaaagctt gcatctgcgg
     6121 taataagccc agcaaaacga aaaatatgag gggctatttt tgtttggctg ttcttattac
     6181 ttgatgcagg acagcttttt cacaacctct gattcacggt gttccagaag tagcttctgc
     6241 gaaaaacaag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
     6301 ttcttgaagc tgaccacttc tgtcacaacc tcttgtaaca agtatttcta ttttaaggct
     6361 gaaccactgt ttctggttca gtgatttctt tgtttttgtc tcgacgagca attgctggca
     6421 agatcatacc taataagatc aagacaattg gtgtgatgat attcgtgaat aattggaacc
     6481 accatgcttt aggatcagca gtataatcta cttttggcac cattcctagg atacatgcaa
     6541 acgcagtgaa ggcaaagcac caagcaccaa agataaaacc gatttttgga tttttaacaa
     6601 atttatattc agaattgaat ttcttataag ctttattcaa catcatatat gccaagaaca
     6661 cccataggta acgcattggc atgacaactg agttcaagtt cgtcaaccat tttactaatt
     6721 cattcatatc tttgatacca aagatcggca acaaaataat gattcctact agtatacctg
     6781 tcagaagata accattcacc aatgtacctt tacgattacg tttacgtagc cagtttggta
     6841 caaattctgg atccgcatct gctaacaaga tttttagtgg tgcatcaatt gaaaaggcta
     6901 atgcggagat ttgtccaatc atgtttgtca atgcgtaaat gatcatcaat gagttaccga
     6961 taccatagta attacctaaa atttgaaaag cactgtatgc cccatttgcc atcaagtctt
     7021 taggaatatt atcgctagca aacagcatac ccattgcgac tgagcctaat acagcacaaa
     7081 tccctaccat tgctgctaag aagatcatcc ctttaggaaa ttcctttgca ggatttcttg
     7141 tagagttgac ataaggggat attttttccg ctcctccaac tgcaaatact agcatcgaaa
     7201 cggttgtgaa ataactgaaa tcaaatgttg gaatatatgt tttcacttga ttcatatgtg
     7261 gtgtggcaaa ttccatcgtt ggattgatga acggtgccgt tacagctaaa agaataaata
     7321 agatcgacat gacgaacatc gctgttccgg ccagtccgcc aatcactttc aaagtagaaa
     7381 gtccttttgt tgataaatat aaaaaggcaa gaaatataat aagtgaaata actgctacca
     7441 tttgtactga catcgtattt acgatatttc catttccttg gactgcccaa ccgccagcaa
     7501 tcaggattgc ttgaggcttt tgtgccaaat aaggaatatg gacgacccag taagtccaag
     7561 cggcatagta tgccagtcgt ttattagatg tcgtattttc gacccatgaa ctgacgcctc
     7621 ctttactatc cttgaaggct gaccctaatt gtccaacgat caatgcgtat ggaataaaat
     7681 aaatggccaa aatcaatagc caagaggtga caactgatac tccttgctga gcatagttat
     7741 taactacatt ccccatgccc caaaccatgt taaatgcaat taacgcaaca gtaaaccagc
     7801 gtaattgttt gttgttcatt cgttctctcc tttatgataa aaatttgtta tccagatgta
     7861 atcatattca atttttaaca aacttacaag ac
//
LOCUS       DACESX010000119         7870 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.119, whole genome shotgun sequence.
ACCESSION   DACESX010000119 DACESX010000000
VERSION     DACESX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.119"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(95..370)
                     /locus_tag="IXF88_002223"
     CDS             complement(95..370)
                     /locus_tag="IXF88_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1508936.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            602..1336
                     /locus_tag="IXF88_002224"
     CDS             602..1336
                     /locus_tag="IXF88_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1508937.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEKFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            1375..2517
                     /locus_tag="IXF88_002225"
     CDS             1375..2517
                     /locus_tag="IXF88_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1508938.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(2612..4003)
                     /gene="sufB"
                     /locus_tag="IXF88_002226"
     CDS             complement(2612..4003)
                     /gene="sufB"
                     /locus_tag="IXF88_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1508939.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(4008..4478)
                     /locus_tag="IXF88_002227"
     CDS             complement(4008..4478)
                     /locus_tag="IXF88_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1508940.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(4465..5700)
                     /locus_tag="IXF88_002228"
     CDS             complement(4465..5700)
                     /locus_tag="IXF88_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1508941.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(5697..6983)
                     /gene="sufD"
                     /locus_tag="IXF88_002229"
     CDS             complement(5697..6983)
                     /gene="sufD"
                     /locus_tag="IXF88_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1508942.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(6996..7769)
                     /gene="sufC"
                     /locus_tag="IXF88_002230"
     CDS             complement(6996..7769)
                     /gene="sufC"
                     /locus_tag="IXF88_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1508943.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
BASE COUNT         2279 a         1614 c         1477 g         2500 t
ORIGIN      
        1 tgtttcctcc ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc
       61 tttattacta tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac
      121 tatcaatatc agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt
      181 attgagaaac atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct
      241 gatagtccat atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca
      301 ctgtaattat ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt
      361 gtagagacat atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct
      421 tcgtattttt tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat
      481 gattaccagt tcatcgacat acacataata cctcgataaa aaaggggtgt caacatcttt
      541 gtaaaattta tttacaacac cccttttttg aggtatacta attttaggag gtgtggtcaa
      601 tatgataaaa cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat
      661 aaaagctata agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa
      721 cggtcaatta attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat
      781 accagagcga ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg
      841 tggcaatgta gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga
      901 tggtataaca tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt
      961 acactctaat ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga
     1021 atttattgct aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat
     1081 cttaaaaata gaagaattat tcatcaatgc tattgtagaa aaatttgaca ataacattct
     1141 tttcctcact agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat
     1201 tcaattgaaa gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa
     1261 caagctagaa caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg
     1321 aggtgatacc atctagtggt aagtactcct tgtaaactaa gaggagatga tttaatggca
     1381 aaaatatcaa agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt
     1441 gccaccgatc cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa
     1501 caagaagctc aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag
     1561 aaagcacctg atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc
     1621 agtgttaaag cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca
     1681 gcatttggta agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat
     1741 gaatggcaca gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta
     1801 cgctacgggg ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca
     1861 cgaaaaaaag agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta
     1921 ctggatgctt ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca
     1981 tacacaggta tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt
     2041 aataaagaac tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt
     2101 caatctccga aaacaagagc ttctagacga gttatctctt tagatactga gactctttct
     2161 atcctcaaca actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact
     2221 agctctaaag agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact
     2281 gttaatgatt ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat
     2341 gggtttagac atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt
     2401 caacaacgat tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca
     2461 aataatgcac ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag
     2521 agctggtcaa aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta
     2581 tatatcaagg gttctagctc ctataacagg tttacccaac actgccttcc atctcataac
     2641 tgatcaatcg attgagttcg actgcatatt ccattggaag ttctttggtg aatggttcaa
     2701 caaagcccat gacgatcatt tcagttgctt cagattcggt caggcctcgg ctcatcagat
     2761 agtagagttg ttcttctgag atttttgaga ctttggcttc gtgttctaaa gagacttggc
     2821 tgttgtggat ctcattgaat gggatcgtat cggatttcga ccattcgtcc atgatgatcg
     2881 tgtcacattc gatatgagag atcgaacccg cactgttttt ggcaaaggtt acttgtcctc
     2941 gatagtttac ttctccgccg tcttttgaaa ttgatttaga gacgatagag ctagatgtat
     3001 ttggtgcatt gtggatcatt tttgcacccg tatcttggat ttggtttgct cctgcaaagg
     3061 cgatggagag catggtgcct cgtgcacctt ttccatctaa gtaaacactt gggtatttca
     3121 tcgttgtttt tgcacctagg tttccgtcga tccattcaac ggttgcgcct tcgtatgctt
     3181 ttgctcgttt ggttactaag ttgtacacat tgtctgacca gttttggatc gttgtgtaac
     3241 ggcaatacgc atcttttctt gtaaagattt ctacgatggc ggcatgcagg ctgttacttg
     3301 aataagtagg tgccgtacaa ccttcgacat agtggatgct tgctccttcg tcaacaatga
     3361 tcagcgtacg ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg
     3421 gaacatcgac tttgacggct tttggcacgt aaataaaggt cccacctgac catacggctg
     3481 aattcagcgc tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag tactctttga
     3541 ataattcagg gtattctttc aaagcagagt cagtgtcagt aaagacgatt cctaactttt
     3601 cgaattcatc tttcatatta tggtacacga cttctgattc atattgtgcg gaagcaccgg
     3661 ctagataagc tcgttcagct tctggtatac caatcttttc aaatgtatct ttgatttttt
     3721 ctggtacgtc ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg
     3781 catcgaaatc gatgtctgaa aggtcggggc cccatgtttg cattggcatt ttgttaaatg
     3841 tttctagaga tttcagacgg aattcaagca tccattctgg ttcgcctttg attcttgaca
     3901 tctcacggat cacttcttct gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat
     3961 cgtgaaaccc gaatttatat tcttctaatt ctggtacgcc catgttctca ctcctcttcc
     4021 ttgtggtaat gatggaaagc tgcggtacca tcgttttcca ttgcttttcc tagtgctttc
     4081 caggcaagtg tcgcacattt gatccgagcg ggaaatttag ctacaccacc aagcatagca
     4141 gcatcgccta attggtcggg atctttgact tcttttcctt ggacaagctg tagaaagtct
     4201 acagttaaat gttctgcttc ttctattgat tttccgatga cggcgtctgt catcatcgag
     4261 gcacttgccg tactgatgga gcagccactg ccgtcaaagg cgatattttt gatgattccg
     4321 tcttttacat cgacttgcaa ttcgatcaca tctccacatg tggggttgtt caattcgatc
     4381 ttgcttgtcg agtcagtcaa gcttccacga tgatgcggat gggaagaatg atccaagatc
     4441 acttgacggt ataaattgtc taatttagat agtcccatgt tggaaaaact cctttgttgc
     4501 catgattgct tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc
     4561 gcgagcagtc gctgatactt caaggtactt caatagcggt tgcgcacaat gatgacctgc
     4621 tctgacggct accccttcca tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc
     4681 caaattgaat gcaacgactc ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa
     4741 tccatcaatt gcttgtaatt ttggcaacac ataagcggct agttcttctt catatcgatg
     4801 gatattgtcc ataccgagtt tttgcaaata atcgattgca tggcctaatg cgatagcgcc
     4861 ggcgatattt ggtgtccctg cttcgaattt ccaaggtagt tctttccatg tactgtcgta
     4921 taattcgaca aaatcgatca tttcgccacc aaattcaaca ggttccattt cttctaacca
     4981 ttttcgttta ccatataaaa caccaatacc tgttggtccg cacattttgt gtccgctgaa
     5041 agcaaagaaa tcacagtcta gtgcttggac atcaatggcc atgtgcgggg cagattgagc
     5101 accatctact acgatgaccc cgccgttttc atggacaagt tcagctaatt cttgaacagg
     5161 attgaagaca cctaagacat tggatacatg agcaatggag acgattttgg ttttgtccgt
     5221 aatctttttc gctgcatcct ccatatccag gaagccttct tctgttagtt cgatatattt
     5281 caagacagca cctgttcgtt ttgccaactg ctgccacggg ataacattag aatgatgttc
     5341 catgtaagag agcactatct cgtctcctgc tttgatatag cggtcgccga aggttctggc
     5401 tacccaattc aagcttgtcg tcgtaccacg agtaaacagc acttctgctg tttcttttgc
     5461 atggatgaac gcccgtactt tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc
     5521 taacgtatgg acaccgcgat gaacattggc gttatcgtgt tcgtaatagt acgctaatgc
     5581 ctggagtact tgttttggct tttgggtcgt cgctgcatta tccagataaa ctaaaggttc
     5641 atcattgacg tcttgaaata agataggaaa atcattgcgt agctttttag catcgatcat
     5701 ccattcaact tcccttcgat tacttcgact aattctttcc gaacttgttc tacagggatt
     5761 gctgttaaga cagaaccgag gaatcctcga atcactaagc gctctgcttc ttctttatgc
     5821 aatcctctac tcatcagata gtacatttct tctggatcaa ctcgtcctac actggcagca
     5881 tgtccagcag ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc accacgtgct
     5941 ttatcagaaa gcatcaagac acgactttct tgttgggcat ctgcaccttt tgcccctttc
     6001 aagatgtggc cgataccatt aaaggtcaat gttccttttt ctcggatcac tccgtgttgc
     6061 aaaatatgcc cgatcgtatg aggggcttta ttggttactc gtgtatcgat tccttggatt
     6121 tgtctgcccg agctgattgc cacgattttg acttctgcgt gtgcaccttc tcctgctaaa
     6181 tctgagtcaa agtcagcgat cacatgaccg tcattcatga caccaattgc ccagtcgaca
     6241 gaagcatctc gtaaaatgtg cccacggcga ttcatataag aagtaatatt ttctcctaat
     6301 tgatctacgg ctgaatattt gatttttgaa ccagctttag cgatgacttc cacgatgata
     6361 ttacctgatg attttgctac ttgttctttg gttgtctgga aacgttccag ataagaaaat
     6421 tcactatgtt catctgccac gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca
     6481 tcttgaatga ataaggactc gagtggttca tcgatcacga catttttagg cacatacagg
     6541 aaaataccgc tgttcataaa agctacatgg agtgcagtca attggtcttc gttcatgttt
     6601 actgcttttt tcatataata ttcttggact agttctggat attcgatcat tgcagtgaac
     6661 aagtcggtaa aaatgactcc tttttctgct aattccacag gcagttgttc aaaaatcgtc
     6721 catgaacctt gttggacaag cacaggattg tctttcattg catcaaatgc cggaatcgtg
     6781 cctgtttcac tgatggtttg tgtttctttc acatcaaata acgaccagcg atggaatttc
     6841 acccgatcga tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc ttgacggaag
     6901 gttctcatcc aatcaggttc gcctttactt aaagaaaaat catttaccgc gtcaagataa
     6961 tcgagccagt ttttttcttt cattcttgct cccccttacg cttcttcttc tttatagtcg
     7021 attcctaatt ctttactgat accagcgtag ccttctgctt ccagacgttt agctaggtca
     7081 gcattccctg tcatgactac tcggccttcc atcatgatgt gaacgacatc aggtgtgata
     7141 tagttcaata atcgttgata gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt
     7201 tcatttactc ctttagcaac gactttcaaa gcatcgatat ccagacctga atcgatttcg
     7261 tctaaaattg caaatgtagg ttctaacatc aacaattgca ggatctcatt ccgtttcttt
     7321 tctccgccag aaaaaccttc gttcagataa cgttcagcca tttcttctgg catatttagt
     7381 aaagccattt tttcatctaa ctttttcaaa aatgccataa cagaaatttt gtcgttttca
     7441 tcacgtttcg cattgatcgc tgcacgcatg aattccgcat tcgtgatacc tggtatctca
     7501 cttggatatt gcatagctaa aaataagcca agtcgcgcac gttcatctac ttccaagtct
     7561 aaaacatttt tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt tggattgccc
     7621 atgatagcgg cagataaggt agattttcct gtgccgttag gtcccatgat ggcatggatt
     7681 tctccggttt tcattgttag gtttacgcct ttaaggattt ctttttcctc gatcgacaca
     7741 tgtagatttt ttatttctaa gacagacatg cattataccc ccaaattctc tttcgttctc
     7801 actcatttta gactaattga gaatgaagcg caatgacctt ccttactaaa atcaaatatc
     7861 ttttatttat
//
LOCUS       DACESX010000120         7846 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.120, whole genome shotgun sequence.
ACCESSION   DACESX010000120 DACESX010000000
VERSION     DACESX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.120"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            156..935
                     /locus_tag="IXF88_002231"
     CDS             156..935
                     /locus_tag="IXF88_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508944.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            976..1920
                     /locus_tag="IXF88_002232"
     CDS             976..1920
                     /locus_tag="IXF88_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1508945.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1936..2715
                     /locus_tag="IXF88_002233"
     CDS             1936..2715
                     /locus_tag="IXF88_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1508946.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            2728..3426
                     /locus_tag="IXF88_002234"
     CDS             2728..3426
                     /locus_tag="IXF88_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1508947.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3454..4218
                     /locus_tag="IXF88_002235"
     CDS             3454..4218
                     /locus_tag="IXF88_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1508948.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            4231..6048
                     /locus_tag="IXF88_002236"
     CDS             4231..6048
                     /locus_tag="IXF88_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1508949.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            6062..7285
                     /locus_tag="IXF88_002237"
     CDS             6062..7285
                     /locus_tag="IXF88_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HAQ1508950.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KITKWLGTSKVVCLNSQTAAAETGLRLLGIGENDEVIVPAYTYSASASVVCHIGANLV
                     LIDCQQDSLEMDYDALEAAITEKTKVIIPVDLGGIPCDYDRIFEIVEKKRNLFQPSNE
                     IQKAYGRIIVMADSAHAFGANWHDQMVGNVADLTTFSFHAVKNFTTAEGGALVWKEHD
                     KLDNEAMYHQCQLLSLHGQSKDALTKTQLGAWEYDIVGPWYKCNMTDVTASIGLAQFE
                     RYPNILKRRKNIIKRYDEAFQQLGIEVLMHYTDDYSSSGHLYITRVPNISSEQRNEII
                     IKMAEQGIACNVHYKPLPMFTAYKNMGFDINNYPNAYAHFVNEITLPLHTKLTDEEVE
                     YVINNYSKIVKDLVG"
     gene            7299..>7846
                     /locus_tag="IXF88_002238"
     CDS             7299..>7846
                     /locus_tag="IXF88_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1508951.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLMLATLLL
                     PAGITSETSILYKDEDKLLHDK"
BASE COUNT         2750 a         1373 c         1632 g         2091 t
ORIGIN      
        1 aaactgtttg tggtatgatt atatagggaa aacctgttta taaatatttc tatgaatata
       61 ttagtttaat tatgctttta aaataaaaag cgctcattct gatgtgaatg aatcacatct
      121 ttatttatct atcatcaata tttaggagtg aatctatgga aaagccgttc gtcaagttat
      181 tggctactgt ggcggtaggc acaggcgcta ttgtgatttg cttgtttgga taccatctca
      241 ataatcagcg gcaacatcat caacggatca attacgcaga atctgccatc accaatcaaa
      301 aagacaccgt gacctcttta tcaaaagaag tagataagct gtacagtaca aaagaaaaaa
      361 ttttcctaaa tcctgaaata acagaagaaa cagtttcaaa tttgtcacat aaactatctt
      421 ctatcaaact ttcagcagat gattatgaca tcaaggaatc agaattacct aaagaagctg
      481 ctgcgatcca agaagaaaag aaagcagtct taacacagtt agaggatgca gaatcaaaac
      541 tgaagatcca aacggctgtc aataaattat ttactaaaaa cgtatcaaac tggcagcaag
      601 ctgtggatga tgtcatcatc aaagaaaagt tggcaagtgc agatgtcgcc catgtacgtg
      661 aaaatatgag cttttttaag gatagtgctt ggaaaacggt cgttatgcaa tatctaggat
      721 tcgctgatac ccaaattgca caagtcacgc aactagatca gctattcgat acgatgctga
      781 aagacggtca agtaacagct actgctactt acgatcaata tttgaccgca ctcagtctca
      841 tcgaacagat ccggaatgaa aaaataagag ccgcatacgc gtcaaaagca gaaacagtcg
      901 ctcagcaaat gggctatagc aatacgagct attaataagc gagaattcaa gcaaggctag
      961 caaacggagg aaactatggc aaaacgaaga agaagatcaa gagggaaaaa gatatttcta
     1021 attattttag caattcttgt cgtattagta ttagcaatcg taggagtagg agcaaaatta
     1081 tatctagatc tctcacattc tattgaaaaa acttacgaat cagtggaacg aacccaagga
     1141 gaacgaacaa aaccagttga tctggacaaa caagaatctt tctctgttct attgatgggg
     1201 attgatacgg gagatttagg tcgggtcgat caaggaagat cagatacgat gatggttgcc
     1261 acagtcagtc ctgaagacaa acagacaacg attgtcagca ttcctcgtga tacgtatgtc
     1321 gacatcgttg gacatgacac aaaagacaag atcaaccacg cttacgcatt cggcggagct
     1381 gccatggcga tggataccgt acaaaaatat gtagacatcc caatcgatca ctatgtttca
     1441 atcaatatga aaggtctaaa agaactcgtt gatgcagttg gtggtatcga agtcaataat
     1501 gacctcacct tctcacaaga tggctatgat tttacgatcg gtaaaatcac attgaacggg
     1561 gatcaggcat tgtcttattc tcgtatgcgt tatgaagatc caaatggtga ctacggcaga
     1621 caagaacgtc aaagaaagat cgtagaaggt gtagcgaaaa aagtgttgag tctgaacggt
     1681 gtaagcaact atcaatccgt tttgaatgct ctagagtcga atatgaaaac agacatgagc
     1741 tttgatgaaa tgaaaaagat tgctttagat tatcgggatg catttggtac gatcaagcaa
     1801 gatcagctgc aaggagaagg cttcatgcaa gacaatatct cataccaaaa agtcagcgat
     1861 caagaacttg atcgtgtgaa aaaagaatta aaagatcaaa tgaatctaga gaataaatag
     1921 gaaagtggag aaaaaatgga agagacaatc agcttgaaag aaatttttga aacattacgc
     1981 aaacacgtga taacaatcct catctcaatg tttgctggct tggccatctc aggcatcgtg
     2041 acgtttttca tcttgacgcc aaaatacagc tcacaagccc aattgatcgt gacactgccg
     2101 caatcagata ctgcaaatgt caatgatgtc aatacgaacc tgcaaatgat caatacgtat
     2161 aaggatatga tcgtcagtga tctagtacta aatgaagtca aagatcgttt agaaacagaa
     2221 gacaatgtaa aaatgactgc tggacaaatc aaagatgcca tctccgtcaa tcagtcacaa
     2281 aattcacaaa tgttctcgat ccaagcaaca agcacaaatg cagtgacggc tcaacaaatc
     2341 gctaatacaa cgtcacaagt attccaagag aacgccaaag acgtgatgaa tgtcgataaa
     2401 atctcgatca tctcaggtgc agttgccaat acgacacctg tttcgccaaa taataaattg
     2461 aatctagtga ttggcttagt gttaggaatg atggtaggcg taggtctagc tttcttattt
     2521 gaattactcg accggacagt aaaagatgaa aaatatgtga cggatacatt gggcttcccg
     2581 atcttaggga ctgtaccaga aatgtcagcg aaagaattga atgctggtat ccgtaaaaaa
     2641 ggaccagctc cgacacaacc aaaagctaca aacgataaaa cagcgccatc acgcagaagc
     2701 cgctcaagag tctaggagga aagaaaaatg gcacgaacac agaaacaaaa agcagcccaa
     2761 actcatgcag tcagtttggt cacactcaca aacccctctt caccaatcgc tgaacaatat
     2821 cgtacgattc ggacgaatat ccagtttgcc tccgcagctg gtcagcaaat taaaacgatt
     2881 gttgtgactt catcaggacc tggagaaggg aaatcgacca ctgcagcaaa cattgccgtt
     2941 gtttttgcca aatctggtca acgggtgctg ctagtcgatg cagacttacg taagcccgtc
     3001 atctataaaa cgttcaagtt gaacaacgca agcggattaa gtaccgttct tagttcatca
     3061 ggaagtgtag cagatgagat ccaacgaaca ccagtcgaga atttgtcgat tctgccaagt
     3121 ggacctaaac caccaaatcc atcagaacta ctaagttcgc caagaatgga tcagatttta
     3181 gcagaagcaa gacaactatt tgatgtagtg atcttcgaca tgccgccagt agttgccgta
     3241 acagatgcac agatcatgtc ttccaaaacg gatggcacct tattggtcgt acgtgaaaat
     3301 atgtcacgga aagaatcatt gactaaagca aaagaattgc tggatatggt acaagcgcga
     3361 gtccttggtg ttgtatacaa tggggcagaa cacagcaaag atgctggcta ttactacggg
     3421 aattaagaaa taaaaagaga ggagacaaga gaaatgatcg atctacattg ccatatcctg
     3481 ccaggaatcg atgacggagc agaaaacata gatgccagta tcgccatggc tgaaaaagcg
     3541 attcaacaag ggatcacaca tattttatgc acaccccatc ataataatgg aaaatacagc
     3601 aatgaaaaat cacaagtcat ttctctagtc gcttcgttac aagcagaatt agagaaaaga
     3661 caattgcctt tgacgttatt ggaaggacaa gaagtacgta ttaccggaac actgatcgaa
     3721 gacatcgacc gagatgaaat tttatttaca gatttagatg atacatatct tctaatcgaa
     3781 tttccgacac tggaagtgcc gttatatgca gaacagctct tttttgagtt gttacaacta
     3841 ggaaagacac cagtgatcgt ccatccagaa cgcaacgcgc attttcgtaa agacccgaat
     3901 catatgctgc cattcctaga tatgggatgc ttggcccaac tcaccgcacc aagttatgtc
     3961 ggtgtatttg gaaaagacat ccagaaaaca gccaaaatga tggtcaaaca taatcttgtg
     4021 cagatggctg cttctgatgc ccatggggtc tctaagcgca ccttttactt aaaagagtgc
     4081 tataagcaga ttgccaagga ctttggtaag gaaaaagtag tacagatgaa acaagtcgca
     4141 aaggatctga tcaacgggga tgaaatcagt tatccagtct atgaagagat aaaaaagaag
     4201 aagtttggtt tattttaggg ggagtgtaag gtgtttgtat taacaagaag aagaaaaatt
     4261 gccacattgg ttgttgtgga cagtttgtta ttgatcgctg caaatattgc agcagctaaa
     4321 ttcatgaagc cgtttgtagc tataccaatg gaccttattt taatctctac cggtttgtcg
     4381 attggttttt atttatttta tggtagttta ttcaaagttt tcactcggat caatcgctat
     4441 acaaatttaa gagagatcat tgcaattttt ggttcactat ctgcaagtga cgtctcaagt
     4501 atcctcatac tgctgttcat taatcggcaa tacagtttac ggttagttat ctttgcttat
     4561 ctgctttcgc tcttattgat catcggtagc cgattgatct ggcgtatcta catcgaaaca
     4621 aaaaatatga gatatgtatc agcggattct gctaaaaata cattgatcgt tggtgctggc
     4681 gaaggcgggc gtattttata caacagcttt ctgggatcca aaacggctca agatatccaa
     4741 gttgtcggat ttgtagatga tgatccgaat aaaagaaata cgtatctttc aggaaaaaaa
     4801 gtattaggtg cattaaaaga tattccggaa ttgattgaaa aatatgacat tcaaatggta
     4861 acgatcgcta tcccttcctt atcaagaaaa aaattacgaa gaatcttcga gttagtcgaa
     4921 tccgctcacg tgaaagtcaa cacgatgcct tctatagaag aacttgcttc aggaaaaatt
     4981 agcgtatcaa aattaaaaac aattgacgta gtggatttac tgggaagaga tgaggtaaag
     5041 ctggatattg aatccatcaa agatcagatc acagacaaag tcattcttgt caccggcgca
     5101 ggaggatcga tcggttcaga aatctgtcgg caaatcattc aattcaatcc tgcaaagcta
     5161 ttgcttttag gacatgggga gaactcgatc tacttgattg accgtgagtt acgcacgcat
     5221 catcaaaatt gtcctacaga aatcgtaccg atcatcgcag atatccaaga tcgcgagaaa
     5281 atcaatgaga tcatggaaca atatcatcca gatatcgtct accatgcagc tgctcataaa
     5341 catgtaccac tgatggaata taatccaaaa gaagcagtca aaaacaatat tttcggtaca
     5401 aaaaatgtag cagaagcagc taaagcagca aaagtgaaaa acttcgtcat ggtatcgaca
     5461 gataaagcta acaatcctcc aaatgtcatg ggttcaacaa aacgaatcgc tgaaatgatc
     5521 gtgactggat tgaatgaaga aggctgtacg aaattctcag ctgtcagatt tgggaatgtc
     5581 cttggttctc gcggtagtgt gattccagta ttcagagaac aaattgctaa aggtggtcca
     5641 atcactgtaa cagactttcg tatgacccga tacttcatga cgattcctga agctagtcgt
     5701 ttagttattc aatctggcgc attagcaaaa ggtggcgaaa tttttattct agatatgagt
     5761 gaaccagtga agattgtaga cctagccaaa aacatgatcc gattgagcgg gtatagcgaa
     5821 gacgaaatcg aaatcattga aacaggtatc cgtccagggg aaaaactata tgaagaacta
     5881 ctgttggata aggaaagaaa tgatgaagca gtttatgaga aaatctttgt aggaaacatc
     5941 aaaggttact caatacaaaa agtgatggat tttgtcaaga gcctgccgca agacgatgaa
     6001 caattggcta aagatatcgt cacttttgca aatgcttcga ataaataggg gggaaatata
     6061 aatgaataaa ttagaaaaac gtcagatacc attttcaccg ccagatatta ctgatttaga
     6121 aataaaaaac gttgtagaag tattaaaatc tggatggata actacgggac caaagacaaa
     6181 aaaattagaa aaaaaaatta caaagtggtt aggaacttct aaagtagttt gtttaaattc
     6241 acaaactgct gctgcagaaa cagggttacg tttgctggga ataggagaaa atgatgaggt
     6301 tatcgtccca gcttatactt attctgccag tgcatcggtt gtatgccaca taggcgcgaa
     6361 cctagtatta atagactgtc aacaagattc tttggagatg gattacgatg cattagaagc
     6421 agcaattacg gagaagacaa aagtgattat tcctgttgat cttggtggta taccttgtga
     6481 ctacgaccgt atttttgaga ttgtggagaa aaaaagaaat ttatttcaac ctagtaatga
     6541 gattcagaaa gcttatggtc gtataattgt tatggcagat tctgctcatg catttggagc
     6601 taattggcat gatcagatgg ttggtaatgt tgctgacttg acaacatttt catttcatgc
     6661 tgttaagaat tttacaactg cagaaggtgg agctttagta tggaaagagc acgacaaact
     6721 agataatgag gcaatgtatc atcagtgcca gctgctttct ttgcatggac agtcaaaaga
     6781 tgcacttact aaaacacagt tgggtgcatg ggaatatgat attgtgggtc catggtataa
     6841 atgtaatatg actgatgtta ctgcatcaat tgggttggca caatttgaac gctatcctaa
     6901 tattctgaaa agaagaaaga atataattaa gaggtatgat gaagcctttc aacaactagg
     6961 aattgaagtt cttatgcatt atacggatga ttatagttct tcaggccatt tatatattac
     7021 tagagtacct aacattagtt ctgagcaaag aaatgaaata attataaaaa tggcagaaca
     7081 agggattgct tgcaacgttc actataaacc acttccaatg tttacagcat ataaaaatat
     7141 ggggtttgat attaataact atcctaatgc ctatgctcat tttgtaaatg agattacatt
     7201 accattgcac acgaaactta cagatgaaga agtagaatac gtaattaata actatagtaa
     7261 gattgtaaag gatttggttg gatgatagga gaggcaaaat gagaaagtgg gaagatttgc
     7321 caaaagaaat gcagaatgat gaaatacggt attattatga tatcctcagt aaaaaaaaat
     7381 tcagcttagt ggtaaaaaga gtatttgata ttttagtgtc tgcaactttg ttactgacac
     7441 tttcatggtt attttttatt cttgcaatag tgataaaaat tgattctcct ggacctgtat
     7501 tttatagaca agaacgtatt actcgttatg gaaaaaaatt taaaatttat aagtttcgta
     7561 ctatggtaat aaatgcagat aaagatggca ctcttgttac tgtgaaaaat gatgatcgta
     7621 ttacaagagt aggaaaggta atccgtaaat atagattaga tgaaattagt cagctaatta
     7681 acgttttggt cggagatatg tcttttgtag gaaccagacc agaagttgga aaatatgtaa
     7741 agtcgtattc accattaatg ttagccacat tattattacc agccgggatt acatcagaaa
     7801 ctagtatttt atacaaagat gaagataaat tgttgcatga taaaaa
//
LOCUS       DACESX010000121         7698 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.121, whole genome shotgun sequence.
ACCESSION   DACESX010000121 DACESX010000000
VERSION     DACESX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.121"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            13..2340
                     /locus_tag="IXF88_002239"
     CDS             13..2340
                     /locus_tag="IXF88_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1508952.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2466..2795
                     /locus_tag="IXF88_002240"
     CDS             2466..2795
                     /locus_tag="IXF88_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1508953.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2835..3839
                     /locus_tag="IXF88_002241"
     CDS             2835..3839
                     /locus_tag="IXF88_002241"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1508954.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3836..5265
                     /locus_tag="IXF88_002242"
                     /pseudo
     CDS             3836..5265
                     /locus_tag="IXF88_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5471..6643
                     /locus_tag="IXF88_002243"
     CDS             5471..6643
                     /locus_tag="IXF88_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1508955.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNKAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6805..7260)
                     /locus_tag="IXF88_002244"
     CDS             complement(6805..7260)
                     /locus_tag="IXF88_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508956.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
BASE COUNT         2603 a         1443 c         1528 g         2124 t
ORIGIN      
        1 ttgagaggag ggatgaaaat gattaagaaa actcaaaata aaatccaacg tatatgtcaa
       61 aaacattttg tttctagact caaagctgta tctaaaagaa aatacttaca tcccattatg
      121 atttcgatca tcttgatttt tactttctta gggagcttat caagtattta tgtaaacgtt
      181 tatacagaaa cgtcaaattt tgctaaccaa acgacagagg agacaaatag tccaccctat
      241 tctgaaatac agtccataga aagcaccaat cagtctagca acaacgagac aacctcaacc
      301 acatcctcga ctcaactgat tgatccgaca gaaaaagaaa tcacagcagc aaaagcagca
      361 ggggagaaag cttattcaga aacaggtaga agccagacat ttagtactgt tgcggccact
      421 gcgaatttca acggacatac cataccagat ggatatacag tgatatatgc caaagatgga
      481 gtcattgttg cggttaacgg atcgatgagt gcggataaaa ctgcgttaca aggcgtaacc
      541 aaagcaatcg cttttattga taaccgtcca gatatccata atctgtttat tggctccaca
      601 gtaaatggta ctactatcac attcccaaaa agtatctata tgggggtggc taccgctgga
      661 aatacgatcg attcagttac acccaatgcg gataaaagtt attcttttac accctcaaca
      721 tggggagggc ttcctgccgg aggaggtatg agtacttttt caatcggtat acagttagca
      781 aatgatattc cattcacggg gtactcgatc aaaaatgggc agatcgttga tggtaaggga
      841 aatgtttcaa attttccaac ctatcaagca gcaacggttc aggtcaatgc gaatcggaca
      901 ggaacttttt cttatacagt caatggtgga gcagcaaaaa tcggaacgta cgggactaca
      961 ttatctggaa tccatgtggg tgacaaagtg gttatcactc caacagcagt aacgggctat
     1021 tcttttgcac aaacaaacgg ttctttcaca gccctagaaa gtaacgtcga tacggtgacc
     1081 tatacaggca aacaaacacc aatcactatt aagtatcaag actattttgg acagatgatt
     1141 gctccttcta aaataatgaa tcttacttac ggaagtgctg cacaagacct gacgactaat
     1201 acgcctacta tcaacggata tacctttaca gctgtttcag cgacagaaac aaagaaccaa
     1261 tcagcaatga gtgttttagc tagtttagat acaaatggaa atgtaatcgt aaaggacgca
     1321 aatggaaaac agatttctga agtcatccta tattataaga caaatgcaac agtatcaatc
     1381 aatgcaaatg gcagtaagta ctatgatggt ctttctgttg taccgatcgt taaatatgcc
     1441 tttaatgata aaacagaatc aacatccctt ttaaacaatg atctcagtat tgatcaaatc
     1501 acatggaatc caactgattt caaagcgatg aatgaaaagg gagttgaagt aactgcgcct
     1561 actgaaattg gaacctatac gtcttggcaa ttgacaaaaa gcggactagc taaactagct
     1621 gccagaacaa actatttatt tactatcgtg cagacatctg atgtctatac gattaagcaa
     1681 atcagcggtg cggtagagct cagtggcagt aagacatatg atggaaaagc aggtgtcccg
     1741 tcaatccatg tcaagctagc agagggtgtc actagttcgc ttactccagt acaagtggca
     1801 cttagcagta tggattacac agcagatgca caatcagcaa aaaatatggt taatgctggc
     1861 agctatacaa ttaaactaac gaatagcgga atagacaaag tcaaagcagc aaatcccaag
     1921 ttgtcattta ctgatatagc caacacatca ggaacttata ccatctataa agctaacgca
     1981 gtgattactg tcgatgacgc tgcctttaat tatgatgccc aaagtcactc gatttcggtt
     2041 ggaaatctcc acgttaccgg tgttgtctta ggcgaatcat tggattatac cctaacagtt
     2101 aacagccgga cagatattgg atcacaaata gtaacgatta gtttaggcga aggaagtgta
     2161 aacaataatt atacgattat cgcaacagat accgctaagt tgacgatcaa tccgactctc
     2221 ttgcttcctt cgacaggtgg gataggtcta ctgccctttg tctttttagg actgatcttt
     2281 attttttctg gcttctttta ttttatccat cgtaagaaaa aagcaggtga acagcgatga
     2341 aaaaaacaaa aaatgtacgt gtggatatat ttgctgttct cttttttatc ctactggtct
     2401 tttttatagg ttctccgact gtgtcagcta gagaatcacc tattacattt actttgcata
     2461 atcgaatgtt cgaaagcgat aagatgccaa gtatcagtca aaacgacgga ataaatatcc
     2521 cggataactt tcaaggagta aaagggttga acggcgttac tttcgctata tatgatgtct
     2581 cggatgaatt ttacaagctg cggtcagaag gaagtagtgt ggaagacgca caaagaaaac
     2641 tagcacaaaa aagtgatagt gaaaaaatac tggcagaaaa tgtgacaaaa acagtagacg
     2701 aggaagaggg cattgcttct tttagtgcat cggacaaaga tgagcaaggt cgtgacgctg
     2761 tttaccgatt tgcggaaata aagacttcag attaagtaaa agaaaaatca gcaccatttg
     2821 tcgttgtgct tcctgtgaca gattcatcta atcataaatt gacgaccatc cacttgtatc
     2881 caaagagcga acaaaaaata cctgctgcac tcacattaac aaaaacagta gagaataagc
     2941 agacagattt tgcagatggg gataaagttc cttatctcat caccgtaaca attcctgaga
     3001 acatcaacga gataggaacc tatacgatca aagatacagc tgatcctcag ttatggctag
     3061 aaccagaaac gatcgatttt ttgattggtg gagacaagat tcatacattt cacacacaaa
     3121 aaacaaaaca tggttttgtc ttatcagatt ctggaaaaga tcttagttcg tttgctggga
     3181 aaaaattaac gatccgttac caaatgacat tgaaagaaaa cagtgaaaaa acgacatttg
     3241 ataacgatgt acgtttgacg actgacaatc aaacagtaga aacacatctt gccatccaaa
     3301 caggaggcaa acaatttgtc aaagtggatc ggcaaacgaa acaaaaacta gcagatgctc
     3361 aatttctagt caaaaaaaat gaccaatact tagcgcaaga aaacgggata aatcattggg
     3421 tagccaaaga tgattcaaga gcaacgattt ttacttccag taaagatgga acattttctg
     3481 tccatggatt gtcttttgga agttacgaac tagtggagat taaaccgcct aaaggatata
     3541 tattgagtca atcaaccatt ctctttgaag ttgaaaaagg cagttatgat ccgcttcatc
     3601 ctataccatt ggaaatcatc aacgaaccaa aagcaaatcc gacgaataat ggaaaaacgc
     3661 cagttcagat gcagacaaat ggtccatcaa atgaaaaaaa tggaggaagc tttccaaaaa
     3721 caagcgaaga gatgcttgga ggtttctcaa ttttagggtt gttgctagtc ctttcaacag
     3781 ggacagcttg gttttataaa aaacaacaaa aaggaaacaa taggagggaa ataaaatgaa
     3841 aaacatcaaa acaaaaatat ggacgttttt aggaactgct atcatgctgc ttcctttcgt
     3901 tctcggccta ggaactgcag aggtttctag gccgtatcac ctacaccaga aaatgttacg
     3961 gttaatttgc ataaattgaa attcacttca gcaccagaaa atcaaataaa caacggaacg
     4021 gaattgacat ttccgaatag cgaaccgttg aacggcgttg aattcaacgt atatgacatc
     4081 actgctacgt actatccaag taaagacaca gcagttccag cagacgctac acccttcgca
     4141 agtgtaacga cttctggtga aggattagct aacctgactc ttccaggtaa atctgatggg
     4201 aaagatgctg tatacgtctt tgtagaaaca ccaaagcccg gtgtcgaaac gtcgccaaat
     4261 atcgtgttaa gcttaccctt ttataacccg aattcatcag accctgacaa acctttagac
     4321 acgattcacc tatatgctaa aaatgtcatc aaagaaagca atatcaaaat ccagaaagct
     4381 gggaactttt caggggtaga taagttagca ggggcgcaat tccagatcaa aaattcagag
     4441 aatcaattcg tgaccggttt tgatgaaaac actggtgtcg ctctctatga tcctgctgag
     4501 tcagcgttga cattcacaac tgatactgaa ggatccatca gtgtcaatgg attaatagac
     4561 ggcaattata ctttagttga aacaaaagca ccagatggtt atcaattaac caatccacaa
     4621 aaagaaacta cttttatagt aaaaaacgga aaattgcaac aaacaaccgg tatcatccat
     4681 ggcgcagatg aagccaatct tgggaatcca aacggtgtag tcaatcggga aaatacaact
     4741 acggataaca cgattactgt agaaaatgtc caaaactacg gtgatttcca cttcatcaaa
     4801 caagatgtga acacgcaaga aaaattagcc gatgcagaat tttctattct aagagcaaaa
     4861 gatccaactg ttaaattaat gaaaaaaata gcaaatcttg cctcaggaga atacccatat
     4921 gcttggtcag atgaagtcac aggttctgac tgggaagaag tgaaattgat ttctgattca
     4981 gaagggaaat tttcaatcga agggctgaaa caaggggcat actgccttca agaaacaaaa
     5041 gcaccagaag gctacgcatt acctaagaat gaagctgcgt acactgaatt tacagtagat
     5101 gatgatccaa caacttctga tgaaacaacg atcgataacc aacctaaagg aatcttgcct
     5161 tcaactggtg gtatggggat catcgtattc atcttggtag gtactgcttt agttggagga
     5221 gcagtgatct acttcaaaaa acgccatcaa gaaacagaag cttaaactat tggaaacaaa
     5281 aatcatacga taaataaacg atagtgattc aaatggggag aaaggttgtg acaagtggtc
     5341 ggtctcttgg tattcgctca acaaacggca aaataggagg aactattttg ccgtttgttg
     5401 agcgaatact caggtcaagg cgctgatttc acaacctctt catatacata ttagaaaata
     5461 ggtggacatg atgaataaaa gcagaataag aatactggac atcttcatgg caatcatttt
     5521 agtcgttggg atcgggatct tttcttatcc ttttgtagaa gattcactga atgattttct
     5581 tgcccagcaa atgatcatcc attatcaaaa gcaagcatcg aataagaatt cagaggaaat
     5641 aaaaaaacag caggaaaaaa tgacaaagaa aaaccaacag ttggcagagc aaaacgtatc
     5701 accagggata ggcagtttca acaaagcagt tgatgcaaaa gcattaaaag atttacctag
     5761 taatgccttt tttatggaac atatgcttgg agtcatcgaa atcccaaaaa tcaatgtcag
     5821 cttacctata ttcgatcaga caacggaaat atttttgcaa aaaggaacgt cattattgga
     5881 agaaagttct tatccgactg gcgggaaaaa cacccatgcc gttctttctg ggcatcgtgg
     5941 actcccagaa gcaaaattat ttacagattt gcctaagttg aaaaaaggag accagttttt
     6001 catccagatc aatggaaaga cattggcgta tcaagtagaa aaaatccaag tggtcttacc
     6061 tgatgaagta gattctttgg gtatccaaaa aggcagagat ctcgtgactt tgcttacctg
     6121 tactccttat atggtcaata cacatcgact gcttgtgacg gggcatcgta ttccttatca
     6181 agcaaaagag gcaaaaaaag cgatccaagg aattgatcaa tggaaaaaat ggaaattttt
     6241 tatttggttt atcggtatac tacttggatc aatcggctta gtttggctac tcatcgctta
     6301 tttagattct ttggccatcg ctaagcgaaa ctatccttta tctttttatg taaagaacac
     6361 aaacggacga ccaatcgaag gaatggtctt ttctgtaaaa acgctcaacg gcaaacacta
     6421 tattactaga gaaaaagttc catttgtgaa agcttctgat gaatacggac tagtgagatt
     6481 ttctgattta aaaggcagga actatcgctt gcaacatgag gagctcctgc ttaaaatcca
     6541 tgtaaaacat aaacattcga aacagttttc aatgaaattg aaaaaaggaa gatataaact
     6601 gcgaaaagaa aaagaggtgt actatttgat tgagaaagaa tgaatgaaga tgtccaaaaa
     6661 agcagaggga acagactaaa tgaaaaaaga gagaagctga cgaaatggac gtgtaaactg
     6721 ctatttttgg tttactatct ggaacgccag cttctatttt tttatgtttc agaagaattt
     6781 tattattata gtataacttt tgaattagtt tccataacat ttggagcaag gggttaaacc
     6841 acgagcttga gcttcgctta aagtggcagg ggtatacgta ccatttccac atttatgtgt
     6901 atggtatttt gaaccggtcg gtgtgacata gacagtttgt tcattatttt gctcaggagc
     6961 tgcggcttgt gcctgctgag aggcctgttc agctgctgct tgctgtcgcg cctgttctgc
     7021 ttgttgctgt gcgattgctt cttgttgcgc ttgttctgct tgttgtcgtg caatttcttc
     7081 ttgctttttc cgttcttctt ctgcttcttt tgctttgatt gtggaatcaa ttgcagcaag
     7141 acggcttgtc aactcttgtt tgttttcagg tagcgcttgg atagctgctg aagcagttgc
     7201 atagttttct tttgtaggat ttgcttcagc ttgtgacaaa gcagattccg cttctgtcac
     7261 ttttttttct agtgcagctt tttccgcttt ttgtttctct tcttcttttt tcttttcaat
     7321 ttgttcttgt tctttcgatg aatctgccgt actttcggag atcgtggtac tgctgctgga
     7381 agcctgagct gaacttgtgc tttgagtagt agacgtagta gcaccacacc cactcaacaa
     7441 acttcctgcg accaaaaaca atgcaactaa ttttttcact aaatttcctc cttaagtaaa
     7501 aaacaaccgt tttcaataaa tgtccttttc aattgtttac ttacttgtta taataattgt
     7561 gttatgaata gtgattgagg aaacttaatc taatcccgtt ttgatacacg ggattttttg
     7621 ctatagttct gttattaagt atgcaataat aaaatatgta cgttatttta ttattagatt
     7681 attgacattt atataata
//
LOCUS       DACESX010000122         7688 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.122, whole genome shotgun sequence.
ACCESSION   DACESX010000122 DACESX010000000
VERSION     DACESX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.122"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..548)
                     /locus_tag="IXF88_002245"
     CDS             complement(<1..548)
                     /locus_tag="IXF88_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1508957.1"
                     /translation="MIYGAIEAGGTKFICAVSTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQRKSKKYGHITSTPKLAWQNFNFVGTLKQHFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAA"
     gene            complement(587..1705)
                     /locus_tag="IXF88_002246"
     CDS             complement(587..1705)
                     /locus_tag="IXF88_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1508958.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(1702..3801)
                     /locus_tag="IXF88_002247"
     CDS             complement(1702..3801)
                     /locus_tag="IXF88_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1508959.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERYPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(3852..4673)
                     /locus_tag="IXF88_002248"
     CDS             complement(3852..4673)
                     /locus_tag="IXF88_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1508960.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(4670..5479)
                     /locus_tag="IXF88_002249"
     CDS             complement(4670..5479)
                     /locus_tag="IXF88_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1508961.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(5500..5994)
                     /locus_tag="IXF88_002250"
     CDS             complement(5500..5994)
                     /locus_tag="IXF88_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1508962.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6006..6494)
                     /locus_tag="IXF88_002251"
     CDS             complement(6006..6494)
                     /locus_tag="IXF88_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1508963.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(6571..>7688)
                     /locus_tag="IXF88_002252"
     CDS             complement(6571..>7688)
                     /locus_tag="IXF88_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1508964.1"
                     /translation="IVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFE
                     ALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLN
                     NLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGIS
                     DKLKDVFRHHLSDESIEVFTKEYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPN
                     INVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIVMS
                     EVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKPDEDEF
                     YYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         2322 a         1775 c         1291 g         2300 t
ORIGIN      
        1 gctgccatcc cctctaaaca atcttggtgg aagggacagt ttcctgaaaa attgtccttt
       61 ggatgtcgct tgatcaacat atgccccatt tccggatgac tgaacccttc cacgaaagtc
      121 ccattgaaca aggcaccacc tccgatacct gttcccacag tgaagtatgc aacacctgct
      181 aaccctttgc cagaaccagc aacatattca ccatagcaag ccgcattaac atctgttgtc
      241 cattcaatcg gaatattgaa atgttgcttt agcgttccta caaaattgaa attctgccaa
      301 gcaagtttcg gtgttgatgt gatatgaccg tatttctttg atttacgttg aatatcaatt
      361 ggaccaaaac agccgacccc gattcctgcc aattgttcct taaaaggttt aaaaaaatca
      421 atgacctgct tcattgtttc aactggagtc gttgtgggaa aactgactct ttccaaaatc
      481 gtcaattcct cagtactgac tgcacaaata aatttagtcc ctccagcttc aattgcccca
      541 tatatcatca taggcttctc ccttcgaata tgtgtaaaac acttcattaa cgcggcaagc
      601 gttctcgatc agtgaaatga cgtttagtat ttcctgaagc ttctgttgaa taccaatagg
      661 cgacactagc aacatcgtct tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt
      721 gtaaagtgac cgataaatct tcggtaaaac agatggggtc ttgaagatgc caacgataaa
      781 gaccatgtcg tggcattgcg tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt
      841 caaaacgatc tcttgtggca tctctatttg attggtaagg ataccctaaa aaggctgttg
      901 aatagttttt cgcatgtgga cgtccgaact catcgtactc atgaaaggcc cacgccccgc
      961 caaaataatc ttctgaacct gttgaggtta cggtcggata ctcactatcc ccatcaatgt
     1021 agaatttaaa ctcgccttcg ccccaccaat aacgttctag gcaggtaagc gcaaggtaag
     1081 tacccacgta atagccttga ccctttaatc gatcaattaa cttataatcc tcctgtaatt
     1141 tggtaatgcg ttcccgatgc caatttgcgt gaaaatatag gctattttct tccaattcgt
     1201 ccaccaaagc gtaatttatc gtataaaaaa atgaggacat atccttcgga tgtaaattct
     1261 cgatcgtaat tttagcggat gtttggaaag gcatttcgaa ataggaatta aagccccctg
     1321 taggattaac cactatcggc aacgagttca cctcgcaacg ctctccaaac ccattacaaa
     1381 agaaatcccc taaaggactt tcaacagcag ggctatccga cccatcccaa taaattctta
     1441 agattaaatc acgtaaaaca aaactgccaa attctgttcg atcacgaatc gtcatccaca
     1501 tatgacgaat tattcccgtt ccagtaatat ccgcaatcac agtttcttca ccagaagtca
     1561 gcggaattgc gccacgacct ttgcggcttg gtcctagctc gcttggcaac atcgcagctt
     1621 tacctttctc gcctgttgga ttttcgggag tgattgctcg actctggatt tttttaaatt
     1681 gggtaatatt ttttagtata ctcatctttt tctcctttga atgtgaatcg tttttccctt
     1741 ttctacgtga agaatttttt tcgtatcact tattttcaat ggcttttctg acaaatttga
     1801 aatactcaac tcggattgcg tacattcaat cgaaatgaag gcttgaccga acttcaaatt
     1861 cttccaagaa aacgttaaat tatcaacagg atcaatttgc aatactcctt ttttgaattt
     1921 caatccgaaa tattgactga tcattaggct gacaaaaaga cccgatgccc agccgcattt
     1981 gccgcagcca aagtctcgca cgacttctcc ttgttgagcc tctaagcgat acggccacca
     2041 ccaccacgaa ccgtctaaat ctgccaaaca aattagttct tctaatcggg ttacgaaagc
     2101 ttgagggttg tgcagctcgc tcattaaaac agtgataaag ccaggaaaag tggcgccgga
     2161 ttctaatccc caagtgatgc ctttgatttc ctcgctatag gttggattga aagaactagc
     2221 agaaaaggcc atggtgttct gatataaatg atctttatta tctaaaaatt gataaaaagg
     2281 catcaacgta gtgtctgatt cttctccatc atgcatcaat aaattaattt ttccatccac
     2341 aatcacatca cttaaaaaga ttaacccttg ctcaagaatc ggtttttgat agtgcttcac
     2401 tgaataggtt gttttctcct catcaccgat tccctccaaa aattgctcac caaaaggacc
     2461 atcgatagtc atttgtttac aaattgattc ctttagttta caggcaatgt ttttgtatcg
     2521 cttgctaaga ctcagttgtt ctagggcttc aaaaatttct gcaagtccaa tacaggcctt
     2581 ccaaagacaa agattgctgc cggtatgata ctttccaagt gcaaaggcgt ctgataacca
     2641 aacagatgga aaaagcattg gttcccctga ccgacgttga gctaatatgt cctcaataat
     2701 tcttgaagca ttcagtagaa cctgcggata ccgctcaaag aattttgtat ccccagtatg
     2761 ttcaaaataa agactagcca gtaacgcact ggataacgaa ttactcagag aatgattgat
     2821 acctcctgta aattttgaac caggaaactt aacgccatat tgatcaaacc atagaaccgt
     2881 tttttgacat aactccggtt caaacatggt gaatggcaat gatgcgtaat acatatcctt
     2941 attccaaatt cgattagtag caggatagct gccccaattg ctgccgacga cctgtccctg
     3001 tcgattcata ccgaaactag aaaagctttg ataaagtgca cgacgataga gatggacaac
     3061 tctatcgttt ggcattgaca gctgcccaaa tagctgagca aaatagttca atgtttcctt
     3121 cagccatttt tcgatatcag attcctgaaa agtcattact tcttcaaaag catttgggtc
     3181 cacaaaagca atggcgaagc ttgccacttc ctgttgtgcg atcgtgtcct tatttccttt
     3241 ttgagaaatc aacacatttt gctgatccga atacttctct tggtacaaag gcacatcttt
     3301 cactgaaact tgaaggcttt cgtgtgtttc attcacaatg taaacctgtt gaacaagcat
     3361 acttaaacgc ttattagcca gtattggagc aaaggatact acaatgacct taaacgtcga
     3421 ataatgatga atatatctag gcaaacagtc ttgaatcaca tcgacttcaa tacgatgatc
     3481 cgattctgac aaatggtaga caatcccgtt tacttcaacc tttaaggata acgaattggt
     3541 ttgagaaagc tgtttattga cccaaacacc tggtttttcc tcagttagta aattttctcg
     3601 ataaaaggtc aacgtttgaa tcgtcccgca aacatcaagg tttcctaatg tcttttgatt
     3661 acttacatcg aaggtcaatc cctttacatc aggaatattc cgatagtagc tgtattcgtt
     3721 tgaagtagtc acatttccgt cgtttaactc agcattaaaa aatgggtgtt tgggtgaata
     3781 aattacttca ttaatagtca tcttttccct ccttgctaaa aaaactgcag cctagtagct
     3841 gcagcttcct actatgcgat tccaagtaat gacaataaaa ttcccaagac gaccattcca
     3901 aaaataattt tgatgacgcc gactttttta tctagtaatt tgaaacagct caatgttacc
     3961 aataacggta caattccttt aaaaatacta tcgaggattt cttggacatt cattagttct
     4021 tcgcccttca ttgaaaagct caaaacggta ttgaatttga ccatactgat cgtcattgca
     4081 cctaccatga tcagacccaa gatactagca gcttttgtca gaatttcaat taacccgttc
     4141 gcatacagct tttcaatata tttagagcct aatgaatagc ctaaaacgcc gccatagtat
     4201 cgcatcaagt aattaggtat attgaacata actaaaaaga taattggtgc caaaatattt
     4261 ccggcattgg ctaagttgat cgctagtccc gcggtaacga cacgccaaac tccccagaac
     4321 aatgaatcgc caattccagc caatggtccc atcaaactcg ttttgatcgc gttgatactt
     4381 tccacgtcaa aaccagcttt ttcagaattt tccttctcca tcgatgccgc aatccccatg
     4441 acaaaggacg cgcaaggaat ggtcgtcgaa aaatactgtg tatgacgttc cagcgccgca
     4501 gctcgatctt cttcactttt gtagaaatga ttaatgaagg gcatcataga ataggcgaaa
     4561 ccaccgccgc ccatcgtcac cggattcacc gaagcataca tagtcatcga gcgccaaaac
     4621 atcttccgaa ccaatttttt ctcatcttta gaaattccat cagctaattt cattgaaaga
     4681 aatcctcctc ttcttggtca agcgttgcat ttacagcagc atcgctttga actgcatgaa
     4741 cagatatatt ttttagttgt agatctcttt gggctataac aattgcaata atcgttccaa
     4801 ctgcggcaat tgcaatcaat ggtaactcga gatacgccat aacaacggcc ccaaagaaaa
     4861 accacacagc aatattttta ctccaaagca tccgtaacag catcgccatc ccaactgccg
     4921 gcagcagatt tccagcgact gttaatccgt tcatgatgac ctctggaata ttatccaaga
     4981 cgcttttgac aacatctgct ccaaataaaa cggcaaagaa tggaataaga gcataaagcg
     5041 catagtggac aaaccacata aagaagtgca gattgatgat tcctttttga tcaccagatt
     5101 ttgcaaattt gaaaaattta gcagccatcg gtccacatac aaaagaaaaa atcatattca
     5161 ttaactgtaa tcctaatact gctagtggaa agacaatggg aataatcgca tccacgtttc
     5221 ctcccatttt gattgcgaac gctgtagcca gcccagcagc caaagaaggc tccgccgtac
     5281 tagagattcc gatattgacg acacccataa agatggcttc tagtgacgcc ccgacaatca
     5341 accccgtctg caaatccccc atcaacgctc ctgctaacgg agcaacaaca atcggacgac
     5401 tcagcatgga catcccgaac atttcatgtc cagcaactcc aataaaaacg gccaaagcga
     5461 ctacaaatgc atcatacata atttttctcc ttcctttttc taaaaatcac tcaaaataga
     5521 gatagctgaa cgtttttcca tcgccggtgt aggctgaaaa acggtatctg gataaagttc
     5581 aactaattct tttaagtctt gaatttctga cgtcgttaac atcacttcct ttgataaggt
     5641 tttcttatct tccgatggcg gtgtcatttt cccagcattc cccacgttca ttaattcgat
     5701 ttccgttggg aaagctttcg caacttttac cgcatctttg acattaggga ctaaaaccat
     5761 caagcgcatg tccttcgccc gcggatcttt taacaaatga atcgcgtcat caactgtttt
     5821 aataatgact ttgacattcg atggtgcagc catcttcaat gtcatctttt gaatatcgtt
     5881 ggtagccgcc tcatcgctgg ctactactaa cccggttaaa tttagactct ttgtccaagt
     5941 catcgctact tgtccatgaa ttaatcgttc atcaattcgc actgctttaa tcatttttct
     6001 tcctcctaaa aaaaatcttc ctcttcattt actacatgta cctcatcaag aatggtcatc
     6061 tttaattcct gttggcattt atttaccagt tcctgaatat cctcctttgt taattccttg
     6121 gttgccaata ctatttcgag cagaattggc agattaaagc cagacaaaat agttacagca
     6181 atctctttct ccaaaaaggt tttggtaatc ttctgattga cacttccgcc aaataaatcg
     6241 gtaaaaacaa aaatctgatc ctgctcgttc acctggttta caaatacatt caactcctgt
     6301 tcaaaatcac actcatccac ataagcatta atcaccgtaa tctcttgaac cttccccgtc
     6361 agtaattgaa tactgctaag aaagcctgaa gctaagtgac cgtgagtcgc tattagaatt
     6421 ttcttcatat ggttaattcc tttctttctg ttcactttta tataagcaat tcctgtgcca
     6481 acttttaaaa acattggttt aatcacgtta attcacatta aaaaaagtgt ctaacctaaa
     6541 aaaaacagat tagacacttt tttagacact ttatttttga atatacggtc ctaataattc
     6601 atagagcagc gaaagctcat aagcattgac tttaaaatta tatttcatct ccattgattg
     6661 aaaaatactt ctagaaacat aataaaattc atcttcatct ggtttcaaat cataattcgg
     6721 ttcttcagat ttttctgtcc gagccaagaa taaacgttcc atcatcaatg ccgtatgcat
     6781 atagagattt agttttacac ggccatttaa agtgaaatga tagtaatttt catatttaga
     6841 aatgactgtt tccacttcac tcatcacgat cgttgggttc agaaagttca aacggtccgc
     6901 aattccttcc aacgaaaaga attttactag ctcctgaatc aagcggttaa attctgccgg
     6961 cttcatttca ggactcaaaa tatgctccaa attggctgct ccatcaccat ctaaaatatc
     7021 atatacattg atattaggaa ttgaaaagga acgtggaaga tccgaagtcg tgatgaccaa
     7081 ttttgtcttg tcaaaataag tttcatcatt ttggtcgatt aattcacgta gttctctgta
     7141 ttccttcgtg aaaacttcga tggactcatc cgataaatga tgacgaaaaa catccttcag
     7201 tttatccgag atacccaatc ccgacatgca ggaaatgatg atgttgctac tttgagaaaa
     7261 gccttcaaag tattgaacat tgattgaata cttgtctgct gcatcctccg caatctgttt
     7321 aaatggcgca ttcatcgtca tcttcaggcc aatatcaagt gcaatggagg tcgtcaaatt
     7381 attcagaaga agtaattctc cttttaaatc gttcttgatc tgtgtgtaga tttgatttaa
     7441 agagcccata tctacgatca gcactagact atctgtattc acttgccgtt tcacgaaatt
     7501 ccttgtcgat tcgacaatct ctgacacatt actctctact ggcatatcca acgcttcaaa
     7561 aacataattg ccgcataatt gattcactac tgcttgaatg ctgctagccg tactatctcc
     7621 gtgagcaagc aacaggccct tcagctcgat ggattcatct acataatcag ataaagtcac
     7681 gacaataa
//
LOCUS       DACESX010000123         7356 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.123, whole genome shotgun sequence.
ACCESSION   DACESX010000123 DACESX010000000
VERSION     DACESX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.123"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            374..643
                     /gene="rpsN"
                     /locus_tag="IXF88_002253"
     CDS             374..643
                     /gene="rpsN"
                     /locus_tag="IXF88_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1508965.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            658..837
                     /gene="rpmF"
                     /locus_tag="IXF88_002254"
     CDS             658..837
                     /gene="rpmF"
                     /locus_tag="IXF88_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1508966.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            869..1246
                     /locus_tag="IXF88_002255"
     CDS             869..1246
                     /locus_tag="IXF88_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508967.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1267..1416
                     /gene="rpmG"
                     /locus_tag="IXF88_002256"
     CDS             1267..1416
                     /gene="rpmG"
                     /locus_tag="IXF88_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1508968.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1440..2423
                     /locus_tag="IXF88_002257"
     CDS             1440..2423
                     /locus_tag="IXF88_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1508969.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2453..2575
                     /locus_tag="IXF88_002258"
     CDS             2453..2575
                     /locus_tag="IXF88_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1508970.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2593..4116
                     /locus_tag="IXF88_002259"
     CDS             2593..4116
                     /locus_tag="IXF88_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1508971.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4140..4379
                     /locus_tag="IXF88_002260"
     CDS             4140..4379
                     /locus_tag="IXF88_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508972.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4572..5207)
                     /locus_tag="IXF88_002261"
     CDS             complement(4572..5207)
                     /locus_tag="IXF88_002261"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1508973.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5228..5446)
                     /locus_tag="IXF88_002262"
     CDS             complement(5228..5446)
                     /locus_tag="IXF88_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1508974.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5755..6582
                     /locus_tag="IXF88_002263"
     CDS             5755..6582
                     /locus_tag="IXF88_002263"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAQ1508975.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6771..7178
                     /locus_tag="IXF88_002264"
     CDS             6771..7178
                     /locus_tag="IXF88_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1508976.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
BASE COUNT         2605 a         1091 c         1440 g         2220 t
ORIGIN      
        1 ataaggaata atcaaagttt caaaaaattg gcgataaacc agttaaacat aaaagattga
       61 ataaaaagtt atagtttctt tcatattgcc gttttaagta gaggaaaagc tacttaaaac
      121 ggtatttgtg tttgattgga ctaataaaag tgaattctta tttttcagag cttaaactgc
      181 atattctttt aaatgatttg attatctcta tgtaccgata aattcgtttt caaatagtta
      241 tctattcata tgagttttat ttcatgttat ttattgttga caagagcaac tcttgttggt
      301 aaagtatcag tgtcaaaacg taacgattac gatttgagat gtctaaatca aaataaaagg
      361 agtataaagg tatatggcaa aaaagtcgaa aattgctaaa gcaaagaaac aaatggcgat
      421 gattgaaaag tacgcagata aacgtcagga attaaaagcc gcaggagatc gcaccgctct
      481 cgctaaattg ccaagagatt caaatccgaa tcgtctgagg cttcgtgatc agacggatgg
      541 tcgtccaaga gggtatatgc gaaagttcgg tatgtcccgt attaagttta gagaacttgc
      601 ccatcaaggg ttgattcctg gagtaaaaaa agcaagttgg taaaaggagg aaatatcatg
      661 gcagtaccag caagaaaaac atctaaggcg aagaaacggt taagaagaac tcatcaaact
      721 gtagtaaaac cagaaatttc atttgacgaa gcgaatggtg attatagacg tagccatcat
      781 gtatctttaa aagggtacta taatggaaag aaagtaatta aaggagcttc aaaatagttt
      841 aatatataac ttttaggagg gaatcaaaat ggaaattatt tacccacctt tagttgaaga
      901 aggattaaag tattatcttg aaacgaccca gcaatcgcta gataaatgca ctttttatcg
      961 ttcgatggta gagagaggaa ttattactga aacgggttta ccgacccaac aggcaattga
     1021 aaatggatta gtcaaagatt actatgagga ccaaggctta tcatttgatg attttttaag
     1081 aatctatcca atttttgaag agtatgacga ggagttattt caatgtattg atggttattg
     1141 ggagatacct gttgatatga aagaaaattt agtttcacaa ttggaatctg gcgagttaac
     1201 ttttgaagat gcacaacaaa ttcaagcata tcttgaagat cgataagtat tgaaggagga
     1261 agatttatgc gtcaaaatat tattttggaa tgtgtcgaaa ctggagaacg tctgtatctc
     1321 accagcaaaa ataaacggaa taaccctgaa cggttagaat taaaaaagta ttcgccaaaa
     1381 ttgcgcagac gggcaatttt caaagaagtg aaatagctaa gtggtaaaaa ggtgacaaaa
     1441 tgggcatacc agttacaatc gtttctgggt ttttaggggc tggaaaaaca acgctaatca
     1501 atcaagcctt gcaagtctca ccctacccca gggaagaaat tattataatt gaaaatgagt
     1561 ttggtgaagt tgggattgat cacaaattat tagttcatag cgaggaacgg gtgttacaac
     1621 taaataatgg atgtatgtgt tgcagtcttc gcagtgattt attagcaata ttgatttctt
     1681 tactggaaac aatagaagaa catcaacaac caatttcaca aatcatcatt gaaactacag
     1741 gtatagcaga tccccaacca atcattcaaa cacttttaac agcgcctcaa ctaagaggaa
     1801 gagtatatat cgatagttta ttgactgtta ttgatagtga cagttttgaa cttattgaaa
     1861 gagaaccaca agcccttaag caacttgtca tagcagatcg catttttgtg tctagaaaac
     1921 aaaacttgaa tttatctgca ctaaaaaaat tacagagaga aatcaaaagt attaaccctt
     1981 tatccgatat tcgaatgttc acacatagtg aaacgattca aaaaaaggat ttttataatt
     2041 tggaaaaatt caatcatccc ttagtttttg aaagtgattc atcagaaaat cactataatg
     2101 gcaaacacta tcatcaacat catcaagaac atgagtttaa atcaattctc ttaacttcag
     2161 atcaagacat aaaaaaagat tttttattcc aattaattga ccggctgatt ttaaccaacc
     2221 aagggagcct gtatcgtttt aaaggattga ttgctttgca agatcaagat tttatagttg
     2281 ccatacaagg ggtaaacgaa caggttaatt ttcaatacac cactcgacaa aaagaccaaa
     2341 ccactattat tctaattgga aaaatgttgg atgaagaaag gatcaaacag agttttgata
     2401 aattactggc ggaaagcaaa taatgataag tattgattag gggtaatttc taatggagaa
     2461 aacggatata tctagtgcat atcgacgact taaaagcccg aatatcaaaa ctagaaaaag
     2521 ggcattaaaa attatcaaag aacataaaca aaataaaatg aagaaattag cataattaag
     2581 gaaggaaaaa gaatgaagaa aatcagtgta ggacttattg ggatagcagc tctaggatta
     2641 ttgggagctt gctccagcac taatgatgca aaagtatcga atgataaaga tggaaaatta
     2701 gaaattgtaa caacattcta tccaatgtat gatttcacca aaaacatcgt cggcgatgaa
     2761 gcgaacgtgg atttaatggt tccagcggga tcagaacctc atgactatga gccctcagct
     2821 aaggatatgg caaaagctca tgatgctgat gtttttgtat atcataacga aaacatggaa
     2881 tcatgggtac caaaagctaa agaaagttgg aagaaagctg ggcctaatgt agtagaagga
     2941 acaaaagaca tgattttatt gccaggtagt gaagaagaag atcatgatca cggagaagaa
     3001 gatcaccatc atgaactaga tcctcatacg tgggtttcac ccaaaatggc aattaaggaa
     3061 gtttcaaata tcaaggatca gttagttaaa ttgtatccga aaaaagcaaa agtatttgag
     3121 acaaatgctg aaaagtattt gactaaactg aaacggttgg atgctgacta taccactagt
     3181 cttaaagaag cgaaacaaaa aagttttgtg acacaacatg cagcctttgg atatttagct
     3241 ttggattatg gattgataca agttcctatt gctggactaa gtcctgaaga agagccatct
     3301 tctggtcgat tagctgaatt aaaagagtat gttaaaaaaa ataagatcaa ttatatttac
     3361 tttgaaaaga atgctaatga taaaatcgca aaaactttgg ccaatgaagc tgggataaaa
     3421 ttggaagtat tgaatcctct tgaaagtctt acaaaagaac aaatggataa tggtgaagat
     3481 tatgtttcag taatggaaga caatctaaaa gcgttagaga aaacaactat ggttgctggt
     3541 gaagaggtag ttccagaaaa agaagcgaag gatgaaaaga cagttgctaa tgggtacttt
     3601 aaagatgccg atgtgaagga tcgcgaattg agtgattata caggcgaatg gcaatctgtt
     3661 tatccgttgt taaaagatgg aattttggat gaagttttcg attacaaagc aaaactcaat
     3721 aaagacatga cagctgccga gtacaaagat tattatacta ctggctataa gacagatatt
     3781 gatactatca acattaaaga caacaccatt gattttgtag tcaacggtga acatcatcaa
     3841 tacacataca aatatgttgg atacaaaatt ttgaattatg aaaaaggaaa ccgtggtgtt
     3901 cgttttaatt ttgaaactga tgatgcaggt gctggtcgtt ttaaatatat tcaatttagc
     3961 gatcatggaa ttgcaccaag taaggcagag catttccata ttttctttgg tggagaaagt
     4021 caagaaaaac tttataatga aatgcataat tggcctactt tctatccagc ttcattatct
     4081 gagcatgaga ttgctcaaga aatgatggcc cattaaaatc caatctttgg aggaaataca
     4141 tggtaagaaa agtattattg tctatggatt ccaagaatat tgatgcttat cggcgttatc
     4201 aatttgcaaa gcaaatgaga ttaaatggga gtacattagc tgatattgct gcaactttac
     4261 atgttagtat tgacacagtt catagagtat taaaacggga tcctgattgt tgttttatca
     4321 aggtcaatac tggaagaagt agacgtttag caaaacggca taatagcttg gccaattaat
     4381 tgataatacc agagtagatc atttttccct tatttggatt tgatctgctt tttattattt
     4441 cttgaattgt caaatcaagc gcaacacttt atcaaagaag acttcataag gtgttctcca
     4501 atctaaacac tttctaggtc tgttattcag tttttcaatc atctgattta tcgttgagtt
     4561 atctaccgta gctatctctt tcgattttgg caagtattct cgaatgagtc tattcgtatt
     4621 ttaatttgtt cattgttgcc aagggcatga ggatctggaa aataaaaggg catattattc
     4681 aatacttctg taactgcgaa atgattggag aattcttttc ctctatctgg agtgatcgtt
     4741 ttgaccgcct catttggaat actgcttaaa agttcaatca ttttctcgct cacataagcg
     4801 gctttctttc tggggacctt ctttcctgct aaaaggaagc atgattttct gtctgtcaaa
     4861 gtcactaaac aagtaccact agttttgcca gctacaatat cggctttcca atgaccaaaa
     4921 tcacttctat catctgcgga ttttggatgg ccatggatcg aatgagtaag gcaaatcttc
     4981 ccacgagact cgacatgatt ttttgtatgt cttgtttttc ctcgatggtg caatgaacgg
     5041 atcaggcctt tattgccgtg tgagaggggt tctgtatcga aaagatcatt gcaaattgca
     5101 cggtagatcg tgataaaact aattgaaaag tccatatgtt catgcttcaa acgattcgag
     5161 attttctctg gcgaccactg aaaatcgaca aataaatgac gaacaattga ccaaactcac
     5221 ggatcattta gaatgtgata acgtccgcat ttttttcttc gataagcata atttttttcc
     5281 gctttggaag gtgattagtt tttttcttgt ttcttctcag ttcatgtaaa atagtaaacg
     5341 ggctatgctg aatgtcgttg ccaattatac taatagaaaa tccttcttcg tgaagcagaa
     5401 agattctttc tcgtttgagt atagtaagat gacaatactg gctcataatt attttccttc
     5461 taggagtgtt ctcgacaaac ttatactagt tggatatcga ttatgactca tttttatgtg
     5521 ttgcacttaa agtgtaaatt caaggtatag aaagaggttc cctatgaaaa aatacctatt
     5581 acaatcttaa ctggctatct tggttcagga aaaacgatac ttctaaatca tattctgtac
     5641 ggtgatcacg gaaaggaaat tgctgtcatt gtaaatgaca taggggaagt aaatgttgat
     5701 ggggtattag tcgacaagaa aggttttaaa agaactggag aaaaacttgt ttcgatgccg
     5761 aatggatgca tttgttgtac attacgcgaa gatttgattg ttgaactaga tcaattagtg
     5821 caattttctg acttgaatca aattgttatt gaagcttcag gaatcagcaa accaattcca
     5881 attattcaag caattatatt agctaaaaac cctttagaca ttgatttgac ccaacgatta
     5941 gagattggtg ccattattat agtagtggat gtaaaacgtt tttgggataa tttcgaaacg
     6001 ggtgaaaaga ttcaggaaag aaaagctgaa ggtaatgtag ttgaagaaga acaggatatt
     6061 cgtggcttgt taattgatca attcgagtgt tgtaacatat tacttttaaa taatagtgac
     6121 ttagtgtcaa ccaagacgat ttacaaaatt aacggtctaa ttgctaagtt acagtccaat
     6181 gccaaaataa ttaggacaat taaaggtgaa gtagacttat cgaaaattct aaatacaaat
     6241 ctttttaata tagatgagac ttcctattca atgggctggt tcaaagagtt agagcttggc
     6301 tatcaaaatc atattcctga aacagaagaa tatggtatct caagttttat ctatcgaaat
     6361 cggttactcc ttgacgcaca aaaatttgcc catcttacgg ataaaaattt tcctggcaat
     6421 attacaagag caaaagggtt tctttggttt gcccaacata gtgatttttc gacgatcttt
     6481 gaaacatcgg gtagccataa aagtattgaa tcattaaatt attgggtagc tagtttacct
     6541 aaaattgagt gtcaaatgat tatacaaaaa aacccgaatt gaaggaagcc tggcatccgt
     6601 tgtttggtga acgcattaat aaactagttt ttattggtat gaagatggat caacaatgta
     6661 ttaaggaaca acttgagggt tgtttaactg attctcttaa tgcagataat ttccgaaaaa
     6721 tttcaccatt tccatggaaa aattctaata tgtttagcca gatatcgtta atgaaatttt
     6781 taagaaaagc tggtgtcaag ataggcgatg tcataattat tggcattttg attattccct
     6841 cttttattcc cttatttgta ttctctatag aaaaggagaa tgaaacaact gaagatgcta
     6901 tcaaatatgc catcgttcaa attgatggca aggaaatgga tcgcttcgat ttggataaga
     6961 tcaatcataa actcgtgaat tatcatcctg cgaagggaca atataatatt gttgagatta
     7021 aagatggaag aattcgagtt aaagaagata atagtcctga tcaaatcgcg gttagaactg
     7081 gatggatctc aaaacccggt caaacgagta tttgtttacc ccataagtta gttattagta
     7141 ttgagaaaaa agaaagcaaa gattactaca tttactaatc tggaaataga tgaggaggtt
     7201 tttaataaga aaaagatatt attattaata atagcagggg ctttattaag tgtgttaagc
     7261 gcttgtggac aagaaaaaga agcggtgaca acaggcacaa gtatctttaa cgggacctgc
     7321 aatggtttag ttaacagtcg gttagattac ttattt
//
LOCUS       DACESX010000124         7318 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.124, whole genome shotgun sequence.
ACCESSION   DACESX010000124 DACESX010000000
VERSION     DACESX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.124"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(231..1679)
                     /gene="cls"
                     /locus_tag="IXF88_002265"
     CDS             complement(231..1679)
                     /gene="cls"
                     /locus_tag="IXF88_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1508977.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1831..2724)
                     /locus_tag="IXF88_002266"
     CDS             complement(1831..2724)
                     /locus_tag="IXF88_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1508978.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2708..3394)
                     /gene="ftsE"
                     /locus_tag="IXF88_002267"
     CDS             complement(2708..3394)
                     /gene="ftsE"
                     /locus_tag="IXF88_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1508979.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3499..4600)
                     /gene="prfB"
                     /locus_tag="IXF88_002268"
     CDS             complement(join(3499..4536,4538..4600))
                     /gene="prfB"
                     /locus_tag="IXF88_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1508980.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4726..7260)
                     /gene="secA"
                     /locus_tag="IXF88_002269"
     CDS             complement(4726..7260)
                     /gene="secA"
                     /locus_tag="IXF88_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1508981.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1948 a         1631 c         1278 g         2461 t
ORIGIN      
        1 aaaaactatc tcattaaaag tttctatgct atactggctt tgaaatgtgt ctcttttaat
       61 agcatatatt agcttatttc ttgctttccc caaaaaacaa gagatacccc aaagttaatt
      121 tcgctcatgc gagacgcttt ctccatttct gacgtactct cagaaatggc cccgttagaa
      181 aaggacacga gatctccatc tgttgatggt tctcatgtcc tttttgtgtt ttaaaggagc
      241 ggcgaaatca atcttgctaa tccttctttc atcttgatga ttattcctcg gttttcatac
      301 atttcttttg tgagatgcgt agaaattttc gagtcttcaa aaaatgcttt tcttacacgt
      361 gaagccattc gttcatcata aatcagtgta ttgacctcaa agtccaagcg aaatgaacga
      421 acatcgatat ttgctgagcc aactgaaaca atgccaccat cgataatcat cgtctttgca
      481 tgaataaatc cattctcata tgtttcgatc cttgctccat actcgatcag ctctgcagcg
      541 aaagaatacg ttgcccagta aacgagaaga tgatctggtt tgttaggtat ttggatatga
      601 acttttacgc ctgaaagaag agccaacttc agtgcttcat gaatcgatcc atccggtatg
      661 taataaggcg tttggatcaa tatctcccgt ttggctaacg aaatcatttt caaataagtc
      721 attttgattt gttcatgttc ggtatctggt ccgcttgtca cgatttgaac agctatatca
      781 ccagtagagt gaatggacgg aaaaaatttg ggagaatagc ctacttcttt tgtatgctga
      841 gaattccagt ccatcaaaaa acggttttgc aagatataca ccgcttctcc gtaaatacgc
      901 aaatgattgt ctcgccaata accaaatttc tttaccaatc ctagatattc atttccaaca
      961 ttgaacccac ctgtatatcc gattttccca tcaataacta cgattttacg atgattgcga
     1021 taattgatcc gaggattaat atacggaaca aatagaggaa agaaaaatgc gatttctccg
     1081 cctgcttttt tcaaattctg aaaaaagctg gaagaaactt gtgtggaacc ccatgcatca
     1141 agaaggactc tgacttttac ccctcgtctt gccgcctctg tcaatgcatc tcgaacttct
     1201 tcacctaatg catcactgcg ataaatataa tactggaagt ggatatggtc ttctgcttgc
     1261 ttgatatctt caatcagcgc atcgaatttt tcacgaccat ctgtaaataa agtgatttca
     1321 tttttagtcg tatagagcgt actttcaaat accgtgagca tatagatcaa ttgtttcaca
     1381 tctacttgtc cagtaggcgg atgaggataa agccctcgca tcaatgcctg tttgtcttct
     1441 tcaatttcca ggttcttacc gattttccct tggatcttca aatcaaagat tttctctttg
     1501 gaaatccctc ttcctaaaaa aatatataaa ataaatccta caactggaat gaacatcaaa
     1561 acgagcagcc atgcccatgt ttgagcagtt tcctttcttt ctcgaaaaac gatgataaag
     1621 gaaagcaaaa tattcataat caaaaggatc gtaaaaaaat tgtcaacgat aattttcacg
     1681 gcttcacctc ttcctctacc aacttaagaa tacccaagtt gttttttaaa gtaaagggaa
     1741 aaaagccgct tccttctatt ctcctagcca ataaaaaaag aaagctggaa cgaaactgtc
     1801 caactttctc attttattta tattgtggta ttagaatttc aagaaacgtc tcatagaaat
     1861 gatcgagcca agtgaaccaa tgatcacacc aatgccgacc attaaaagat tgattttcca
     1921 aacgatattt tgtggatgaa tcaatgaata atgggaacgt aataatgatg gattgatcaa
     1981 ttggaaaaac catgcataac caaatgtgat gatcaaaatc ggaagaatcg caccgatcag
     2041 accgatccag ccaccttcaa ggaaaaacgg ccagcgaata tatccatttt ttgcaccgac
     2101 taaacgcatg atttgaattt cacgctgtct agacaaaatc gtgatccgaa tcgtgttcga
     2161 aatgagaaac atcgctacga acagcagcaa tgccgctgca actaatcccc atgtccgaac
     2221 tgcaccagcg atttggaaaa tccgatctga ggaagctcca ccgtaatcag aacgagaaac
     2281 attcttcaaa tctgctgctt cttctgctac ttgttttgta taagaaggat cagtagcact
     2341 tactacatac acatcatata acgggttatt gtcaccttca aacaatttcc aggaatcacc
     2401 catttcgttt tggatttttt tcaattcctg ttctttactt gaaaattcaa cttttttaac
     2461 atgatctaag tcattcaatt ctttttctag ctttttcatc tcttcgtctt ttgtaccgat
     2521 atctacgaat acagatacat caacgtttcc ttctatatcc tgagctaatt tagttgcatt
     2581 catgataact gccatgaata caccgactaa agtcaacgtg atcgttaccg cactgactga
     2641 agcaactgtc atccagccgt tacgtttcaa actcttgata ctttctaata aatggcggaa
     2701 aaaggttcta atcatcgtat ccatattccc cttccgcttg atctcgaata atgcggccgt
     2761 tttcgatagc aattactcgg tgccgaatcg tatttacgat cgtactgtta tgtgtagcca
     2821 tcacgacagt agttccttgg gcattgatcc gatctaataa tttcatgatt tcccatgagt
     2881 tttctggatc cagatttcct gtcggttcat ccgcgataag tactttaggc gtattcacga
     2941 ttgcccgagc aatagagaca cgttgctgtt ccccaccgga taattcgcta ggaaagactc
     3001 gaactttgtg tttcaaacca acaaggtcca gcacttccat tactcgtttt ttgatatctc
     3061 gaggttttct gccgataact tgcatagcgt acgcaacatt ttcatatact gtttttcttg
     3121 gtaataattt atagtcttgg aacaccacgc cgatttctct gcgtagataa ggaacttcgc
     3181 ggtttttgat tgctattaga tcatgacctg cgacgttcaa agttcctttt gaagcttttt
     3241 cttctcgata catcaatttg atgaatgtag atttaccggc tccagacgga ccaaccacat
     3301 agacaaactc tccttgttcg atgacaatcg agagattgcg gatagcggtc gtaccatttg
     3361 agtacttctt cattacatcc ttcatttcaa tcatggcgtt ctctccatat ctttttcagt
     3421 ctttagctat tatagcatgg caatgtttca acatgcttgc aaaatcttac aattttattt
     3481 caatttaaca atgggttctt attgtagatg catttttaaa tagccgtcaa taaataaatc
     3541 aaggtcaccg tccataactg cttggacatt tcctgtctct acgtttgtcc gatggtcttt
     3601 taccatagaa tagggatgga aaacataaga acggatctgt gaaccccagc cgatttcttt
     3661 ttgttctcct cgaagtgcag ctgcttcttg ttctttcttt tccacttcta attgataaag
     3721 cttagctttt aacatcccca tcgcttgctc acggtttttc aattgtgagc gttgtgcctg
     3781 actagctact actgttcctg taggcaagtg agtgatccgt acagcagatt ctgttttatt
     3841 gatgtgctga ccacctgcac cacttgctcg gtacgtatcg attttcaaat catctggatt
     3901 gatttctata tcgatcgttt catcaagctc tggcatcaca tctaccgaac aaaaagaagt
     3961 atgacgtcgt ttcgctgagt caaaaggcga aatccgtaca aggcgatgaa cacctttttc
     4021 tgattttaga taaccaaaag cgttatgtcc tttaatcagc agggtcacac tcttgatccc
     4081 tgcttcatca cctgcttgat aatctagtgt ttctacttga tagccatgct gctctgccca
     4141 acgtgtatac atacgtaaaa gcatgctgcc ccagtcttgg gactccgttc cgccagcccc
     4201 tggatgcaat tcgacgatcg cattgtttcg atcataaggt ccgtctaaca gcatagataa
     4261 ttcatacgtc ttcatttttt catctaatgc attgatacgt gtttctgctt cttcttgcat
     4321 tgctgcatca ggttcttctt gcagcatctc caccatcaat tcgatttctt ctagttcttc
     4381 ggcaagagaa tggaattggt cataggtttc tttattagcg ttattttcac tgatcacttt
     4441 ttgtgcggct tcgctgtcat cccaaaaacc aggttcagcc atttttgctt ctgcttccgc
     4501 gatgtcttct tccagctgat ctaagtcaaa gagacctcct gaaacttgtg accttttcct
     4561 gcatttctgt caggagattt cgaatttcgg atatttccat gttttttcct tctttcacta
     4621 tacttttttc attatatcta gtctaacaga atcagcaaat aacagaaaga aggtgagacg
     4681 aattcttaaa aaaagaattt gtctcaccaa tcctgctgtc agaaattagt ttgcattttt
     4741 tccatgacag ttcttatatt ttttcccgct tccacaagga caaggatcat ttcttccgac
     4801 cttgttcaca tggattggct gttttttcgc gctagtatta ctattttctt tggcatctcc
     4861 gtcttctgag tgagaagctg ttccttgaga aacctgttca cgctgtacgt tttgacggat
     4921 ctcagctttc ataaagattc gagtgacttc atactcgatg gcgcctacca tatcttcaaa
     4981 catcttatag ccttctgttt gatattcgac taatggattg ttctgtccat aagcacgtaa
     5041 gccgattgac tgacgtaatt gatccattgc gtcaatatgg tctgtccatt tagagtccac
     5101 aacacgcaag atcacgactt tttcaaattc caacagttgc tctggaccat ttagttgagc
     5161 cgctttggta ttaaatactt cttgtgctcg ttcgtttaga tactctttga tctcctgagc
     5221 tgtttttcct tcgatatctg caagagtaat actatcttca tggacaagag tactgccggc
     5281 aaaatcgatg atactttcca aattccaatt ttctttctct aattgtgtat gcgcatctac
     5341 tacacgactg attgtccgtt tgaccatgtt cattagtggc ttggataagc ttttttcttc
     5401 catgattact tcttgtcgct gtttatagat tacttcacgt tgttcacgca tcacatcatc
     5461 gtattgtaag acgtttttac gtgtgtcata gttatttcct tctacacgtt tttgtgcaga
     5521 ttctacttgt ttcgtcagca tcttgctttg gataacagca tcttcctcac cgatcttcat
     5581 ccgatctaag aaagctttga ttcgttctga accgaatcgt ttcatcaaat catcttccaa
     5641 tgacaagtag aactgtgaca taccagggtc tccttgacgt cctgcacgtc ctcgaagctg
     5701 gttatcgata cgacgagatt catgtcgttc tgtcccgata actgctagac caccaagttc
     5761 tcttacccca agtcctaact tgatatctgt tccccgacca gccatgttgg tagcaatcgt
     5821 cacagcacct ttttgaccag cattcatgat gatctctgct tctttaaagt gatttttcgc
     5881 attcagcact tcatgaggaa tacgttcttg attcagcata tctgataata actcagatgt
     5941 ttcaaccgca acagttccga ctaacacagg ttgacctttg cggtagcgat ctttgatatc
     6001 ttgcacgaca gcgtggaact tgctttgtaa tgttgggtaa agcaaatctg gacgatcatc
     6061 acggataacc ggacgatttg tcggaatctg gtatacttgg atgttataaa tttctctgaa
     6121 ttcttcttct tctgttttag ctgtaccagt cattcccgca agtttcttgt acatacggaa
     6181 atagttctgg aaagtgatcg tcgccatggt tttcgtttcg tcttcgatct ctacgccttc
     6241 tttagcttcg attgcttggt gaagtccatc tgaataacgg cgtccatcca tgatccgacc
     6301 agtgaactgg tcaacaatca gtacttttcc gtcttggaca acataatcga tatctaaaag
     6361 catgatatag ttggctctca atgcttgatc caagtgatgg gtcaatgcag tattttcaat
     6421 gtcgtataag ttatccaaac cgaatgtttc ttctgctttt tcgatccctg cttcggttaa
     6481 actgattgtt tttgattgta catcgatttt ataatcttct tcttctttca aacgtttcac
     6541 gaagttgtct gctcttgtat ataaagctgt ggatttttct gcttgccctg aaatgatcag
     6601 cggcgttctc gcttcatcaa tcaagattga gtcaacttca tcgacaatgg cataattcag
     6661 cggacgttgt accatttgat ggcggtaaac gaccatattg tctctcaagt agtcaaatcc
     6721 taattcatta ttcgtactat aagtgatatc acaattataa gcctcgcgtt tttcatccga
     6781 agattttgag ttgatattca accccactgt taatcctaag aaattataaa gttcgcccat
     6841 ttccgtcgag tcacgggttg ctaaatattc attgacggta actacgtgta ccccttcgcc
     6901 agtcagagca tttagatata ctggcatcgt tgcggtcaag gttttacctt caccagtacg
     6961 catttcaggg atatttccat cgtgaagcac gattccaccc atcaattgta cgtggtatgg
     7021 atataaacct aatacacgtt ttgctgcttc tcggacgact gcaaaagctt ccggaagtaa
     7081 ttgatctaat gtttctcctt tttggtatct gcccttgaat tcagcagttt ttgcctgtaa
     7141 ttgttcatca gttaatgctg ccatttggtc agcgtatgct tcaacttttt ttgcaatgct
     7201 atctaaacgt tttagttctt tcttatcgtt ttcgataagt gaacgaatca aattggccat
     7261 gcgttgtgta atctcctttt ccttctgtgt attatccaga ctatgtagac tttacttt
//
LOCUS       DACESX010000125         7307 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.125, whole genome shotgun sequence.
ACCESSION   DACESX010000125 DACESX010000000
VERSION     DACESX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.125"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..639
                     /locus_tag="IXF88_002270"
     CDS             <1..639
                     /locus_tag="IXF88_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1508982.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSK
                     EEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQI
                     KRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
     gene            768..1589
                     /locus_tag="IXF88_002271"
     CDS             768..1589
                     /locus_tag="IXF88_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1508983.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            1609..2169
                     /locus_tag="IXF88_002272"
     CDS             1609..2169
                     /locus_tag="IXF88_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAQ1508984.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            3058..3825
                     /locus_tag="IXF88_002273"
     CDS             3058..3825
                     /locus_tag="IXF88_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ1508985.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3851..4396
                     /locus_tag="IXF88_002274"
     CDS             3851..4396
                     /locus_tag="IXF88_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508986.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFDGQQIGLKKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            4397..4963
                     /locus_tag="IXF88_002275"
     CDS             4397..4963
                     /locus_tag="IXF88_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508987.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            4978..5226
                     /locus_tag="IXF88_002276"
     CDS             4978..5226
                     /locus_tag="IXF88_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508988.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            5246..5485
                     /locus_tag="IXF88_002277"
     CDS             5246..5485
                     /locus_tag="IXF88_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508989.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEQLLRKAQQKGYPDKMLKQIGYYT
                     PEERIEKESCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            5502..6086
                     /locus_tag="IXF88_002278"
     CDS             5502..6086
                     /locus_tag="IXF88_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508990.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEQELILERQSILCSEQDSKRLYGLYDLQLNGLKAQYV
                     ADSFDDMVGQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSIVES"
     gene            6193..6474
                     /locus_tag="IXF88_002279"
     CDS             6193..6474
                     /locus_tag="IXF88_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ1508991.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFNEA"
     gene            6464..6805
                     /locus_tag="IXF88_002280"
     CDS             6464..6805
                     /locus_tag="IXF88_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1508992.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWVDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIERLQRKV"
BASE COUNT         2735 a          987 c         1490 g         2095 t
ORIGIN      
        1 attgccattg gtatacgaga agacggcact aaagaagtac tgagttatgc gattgctcca
       61 actgaatcaa catacgtttg gaatgagctg ctacaggata ttaactccag aggagttcaa
      121 gaagtcttgc tttttattac ggacggctta aaaggcatga aagatactat ccatcaaatt
      181 tatcctaaag caaaatatca gcattgttgt atccatgtat ctcgtaacat cgctcataaa
      241 gtacgtgtca aagaccgaaa agaaatctgt gatgacttta aggctgttta tcaagctaac
      301 tcaaaagaag aagcgaatac cttcttatcc ggcatgattg agaaatggaa gaaaaactat
      361 cctaaagtga cgcagtcact catagaaaac caagacttat taacttttta tgattttcca
      421 cctagcattc gtagaaccat ttactcaacc aatctaatcg agtctttcaa taagcaaatt
      481 aaaagataca gccgtagaaa agagcagttt caaaatgaag aatcactaga acgctttcta
      541 gtcagcattt ttgatacata caatcaaaaa tttctaaaca gaagccataa aggttttcaa
      601 caggtaaccg atacattagt ttcaatgttt actgagtaac taattatttt gcaggaggac
      661 aatttattta cacaaaatta ttgacgctcc caataaattg cttgaagtat tatatttaga
      721 tatcataatt tatggaagaa aaattaatta aaaaggatga gattataatg gggttactgg
      781 ttccgagagc taatacttat actatacctt taacgaaacg ccaattaatt gctaaaagat
      841 atcaaaggat tactagagcc atcaatagag agttttggaa ttctgaaagt gatactgctc
      901 acagtttata cgtaggttct tatgggagag gaacggcaat tagtactagt gatatagata
      961 taatagttga attaccgatg gcagaatttg acagattcaa aaattattta tcaaatggcc
     1021 cgtctaagtt attacaagtt ataaaaaacg catttcagga aatactacca aatagtgata
     1081 ttagagctga tggacaagtt gttaaaataa atttccatga tggaatcaaa tttgaaatag
     1141 ttccagcttt taatgaaaaa gattattggg gtgagtcaaa agggtttatt tatccagatt
     1201 caaacatggg agggaactgg aaagctacaa atcccaaaaa agagcaagaa gccatgaaat
     1261 taaagaatac aaaaagtaat aatttattat atgctacgtg taaacatttt cgacatgtac
     1321 gagatacaga atttacgagt tatcatcttt caggaatagt aattgacagt ttcgtatatg
     1381 aggctatggg aaattggaaa ttcgttgaaa ataacagtgg gggtcaaaac attagttctg
     1441 tatcttatga gacagcttta ttagaatact ataactcaca taaagttatg ggcggtttaa
     1501 atttatactc tcctggaagc aatcaatttg ttaattctga tagtagtatc atatgtttag
     1561 aaaaagtatt aaaaaaaata gctttataaa gttttatgga ggaacaacat gggagacgaa
     1621 aaaaagcgct tggaagttca attacgtgaa gcatatggac gagtaacgta tacttacacc
     1681 actcatttaa agcaagccga taatattatt tctgacaata aaattttaag gtgtcttcaa
     1741 attggtttgt cagcccttac tactggaggg gtacttagta ccattattac tgataatagt
     1801 gagttaacct ttgttgctgg attatgttca acaatttctc ttggattaaa cttatacttt
     1861 aagaattttg atcttgctga agaagctaaa aatcatcaaa ttgttgccga taaattatgg
     1921 ttgatcagag aagaatacgt aagcttacta acagatttaa atcgtttaag tgttgaagat
     1981 attgtgaaaa aaagagatca attaatgaac gaaactcatg aagcatataa aaatgcccct
     2041 aaaacaaata gtaaaagcta tgaatcagct cagaaagcat taaaatctga agaagagcaa
     2101 ttttttacag aagaggaatt ggataagatg ttaccagagc atcttagaaa aaagagttct
     2161 aaatattgaa ctcttttttt atgcttaaaa ttctcttgta atttttctat tcatctatat
     2221 ttaagaagaa atatgatgat agaaacatct cctaaagcca ccgcaagccg ttctaagcat
     2281 agcttagccc gacattgcta gatatgctca tctcaccatg aacgtttcct aaagccaccg
     2341 caagccgtcc tagccattgc ctagtccgac atcgctagat tagtgcatct cgccataaaa
     2401 gttttctaaa gccaccgcaa gccgttctaa gcatagctta gcccgacatt gctagatatg
     2461 ctcatctcac catgaacgtt tcctaaagcc accgcaaacc gtcctagcca tcgcctagtc
     2521 cgattttgct tgatcggttc catttctgtt tatttagtgg gtcaacaccg taatcttaaa
     2581 tagccatcgc ctatttaaga ttactaggtc tgctttgaag gtaaaaagtc tttcattctt
     2641 caagactttt aggctttcaa gctacgcctg aaagcattga aattcatcgc tctttatttg
     2701 gttttgccat ttataattcc ttacgcccat tatctttttc gcctgtcata ccttgtcaga
     2761 agtgtttaga gcttatctca tctttggatt aatacaagtg gcgtaaattt ttttctaaac
     2821 gcaagcgtta cgaaaataat ttacttaaac acttgtatta atccggcaga taagaacgca
     2881 ttctaatcac tcccgacaag gcaagacagg cgaaaaagat aaataaatga acgaaaggaa
     2941 gaaacataaa atgcaaaacc aaatagcgat gaagaaaaaa ccacctccct ctaaaacaat
     3001 ccaaacaaaa aagaattttg aaaaaccaaa aatcaaaaac agaaagaagc gttcaacatg
     3061 agagatagaa ttttagaagt taatcaagaa atggtaaaca agttaacaaa tatcagtggg
     3121 aactatgaaa agtacgatgg tactttactt gataactata tttttcacga tacacaaaat
     3181 atcagaattg gcagaagtaa accgagagcg ttcattctca tcaaagagaa atatttgaat
     3241 gaatggaata gcacctatga aatggttatg actgataact ttgaaaaggt attggatttc
     3301 agaaatgctt tgttgggttc tgatatggga aatagcagtc ttgaatctat tgaagttgaa
     3361 aacaagaccg agaatgaagg tgaaggattg attaagaatg tcttgaaaat cgaacaaaaa
     3421 tatatcagtg aagatagaag tttttataga gtcacaagct cggttggtct agcaaacttt
     3481 gctactaact taattggaaa caatgctagt gaatctttgc tagtgatgtg tctgaataca
     3541 aagaatgagg tcattgctta tagcgaagtt ttcaaaggtg gaattaatca atcagtagcg
     3601 ataccaagag agatttttca actagctttg ctaaacaatt cagcaaaaat cgcagtatca
     3661 cataatcatc cgagcggata tgaatttcca agtgaaaatg acaaaagttt tactacgaat
     3721 ttgaaaaacg caggaaaaac aattggtatt gaattgttag accatatcat cgtaacaagt
     3781 aaaaacacaa gctattactc atttcaagaa aatggttctt tataaaaatt agagaaaaga
     3841 ggaaaacaag atgggtgaaa gaacacaagc gttaattaca gtcaaatatg gtaacagcaa
     3901 aacgaaagca agcgtgtcaa ttcattatca gtggggatat ggtcgataca tgttgatgga
     3961 cgttttgaat caaggaatat atttaatcaa taaagcatat gacttacgag atgaatcaag
     4021 agaagaatta gcaaaaggaa tctttgatag aagtacagga gttatcaatt ttgtcaccta
     4081 tgaatctgga gtgagtgctt atgatgaaac agggtttggt gggcgctatg aaccttatca
     4141 agaaacgatt gaagatgtga tgaagttttc agataacaat gatggctatg ctcatttaga
     4201 gatggtgttc gatgaaaatg gatttagtct agatgataaa accagattgc gattgtttga
     4261 ttttgatggt caacagatag gactaaaaaa gttttgtgaa agcggaaaag agtttgctac
     4321 gaaagatttt attaagggat acagattatt gctagaaaat tatggaataa acctagaaga
     4381 agagagcgtg aaataagtgt ttgaaaaaag aaaagaccag ttccctaatt tagaagcgaa
     4441 aagtcaaaaa gtgattgatg gtgaaatgat tgaattattc caaaaaagtt atggaaccat
     4501 agaaactacc gcaagttgta aatactactt tcaaggaaag agaaacacgt ttaaaggctt
     4561 tgccgattct gatgaagaat tggcaaagaa aatcattgat tggcttgtca gcagtgaaca
     4621 cgtagcaaga gaagataccg ttagaacaac gattaatggg gaagtcctaa caggtactag
     4681 atgtgtttgt tgtaatcatg aatttgtaga agatgaaggt tgctaccgaa tcaatgaata
     4741 tggtgatttt atttgccaga gttgccacga gatagaattt ccaaccgaaa gaagttggtt
     4801 tgaatacatg ctacaagatt atgagatgga aaaagaaaat ggtgaagtag ttttttgtac
     4861 caaagaggaa ttgagtaaga gaagcgatga tgaaattgaa gattggtgtg tgtggttagt
     4921 aggggcagat gattgcgttg catattacac aacagcagaa taggagtgtg aatcagcatg
     4981 gaaggaatgg attttctaga tcatgaagat ttggttgatt ttggttatac atggaaagga
     5041 atggtaggta ttagcagaag tcttgctaat gccttttacg aaagaaatta tgcagtgtat
     5101 gttctttatg atgacgatac agaaagcctt gttgacgagg aatataaatt ggatttagag
     5161 aatgttttat atggaataga aaaggaagat ttagcaaaat atattttttc atggttgggt
     5221 caataagtga aaggaagata gaaacatgac aaaatttaaa agtaagggta tgcaatggtg
     5281 ttcttattat tatgttggga atgatgaaca gcttttgaga aaagcccaac aaaaaggtta
     5341 tccagataag atgttaaaac agattggcta ttacacgcca gaagagagga tagaaaaaga
     5401 atcttgtgag gtactttttg atagtgaaca gctattagtt ttgttaactc aaaataaatt
     5461 aatttgtcaa tttgagaata attaatatgg ggtgaataac catgaatgag aataagatgg
     5521 tattaagtga atacgcaaga ttgagaacca ttaattggtt gaatggtatg caaagtagtg
     5581 gcgcttttga tggatttgat gaagagaata tggactttga aagtttatcg aatgaggata
     5641 ttagatatta ttttatcgaa tggtatttta cagaaccaga tgaagaggtg gaagatgaac
     5701 aagaattaat tttagaacgt caaagtattt tatgcagtga acaagatagt aagaggttgt
     5761 atggtttata tgacttgcaa ctgaatggat taaaagcgca gtacgttgct gatagtttcg
     5821 atgatatggt aggacagggg gcagataggg ttgtcactta ttcagaagat gatgaagagg
     5881 aatgggaaaa ctattcagat gatgagatat gctttaatga tgggtatatg cctattgaaa
     5941 tcaccgaaga agaatatgat aaagtgtccg atagtgatga gattggtatg tgtacggttg
     6001 taagtggatt ggatatagat gtccaaagag tagaggttgc ataccagaag atgaaagaac
     6061 gtataaaaag tatagtagaa tcttgattta attggattgt ttgcaaagta tctaattaag
     6121 agtataatat aatcataaat gtagttaatg gatacaataa tgagtatatt atacttaaaa
     6181 gaggtgaacg aaatgaaggt aaaaaatcca actgaaaaaa gcagaatcca agtcggaata
     6241 gacaagaatt taaaagagaa cgcagaaatg atattggaag agctaggttt aaatcctaca
     6301 acagcaatca caatccttta taagcaagtg gtcgctagag gtgagtttcc agtagagata
     6361 aagctaagtg aagaggaaaa gcaaggaatc agattgcaac agctaactaa ggatatgcca
     6421 gttgatgttc tagatacaga tgaaaagtta gaagagtggt tcaatgaagc ctaatgagat
     6481 agtaacggtc tatgttgcat ttacagataa aaatggtggt aaaagaagac ctattttggt
     6541 ggttagtgat aaagaagata gagtagaatt ttttggaatc acaagtcaat atgagaagaa
     6601 atcagaccaa ataaagagag tgtattttcc tattgcagaa tgggaaaaag caggacttaa
     6661 aaagcaatca tgggttgatg tagggtcttt aaaggctatt cctaaaagcc gagagaacat
     6721 atcatttaaa aaggttggta tgctatcagc tagtgatacg aaaaagctaa ataggtttat
     6781 cgagagatta caaagaaaag tctaacaagg cttttctttt tttgattata tgtatattaa
     6841 gatatacgtt tgtataaacg tatttatgta tataaaaata aaagtaaata tatacgtgat
     6901 tataaatatt aagaaaacat taagaattgt ttaatagtaa taattagtaa tatacgaatt
     6961 ataagatgta atttatattg aatgtatgta aataaataaa atggctgacg gatattgaat
     7021 agcaataaat aacaaaaagc cagacggata cagtgagtga agtagcttgc tacgaacgaa
     7081 cagacagaat gaaacggcag tttcattaga gcaagtcgct tgcgacgtag cgttaccgct
     7141 gatgtaactg gtaagttaca tagagagagt ttgcttgcaa acgaacgtta cctacttgaa
     7201 tagaaataaa aaaataacat attagtgaat tttgaacagg agccacaact cacccaaccg
     7261 cttgcggtgg gtgagtccca cggaggagaa gacgaacgaa cgctcgc
//
LOCUS       DACESX010000126         7172 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.126, whole genome shotgun sequence.
ACCESSION   DACESX010000126 DACESX010000000
VERSION     DACESX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.126"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..881
                     /locus_tag="IXF88_002281"
     CDS             <1..881
                     /locus_tag="IXF88_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1508993.1"
                     /translation="ILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQVY
                     KKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIEND
                     ANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLNGD
                     KTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTKGL
                     FSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRNDAN
                     LVGAAANYLAVRQGEN"
     gene            998..1231
                     /locus_tag="IXF88_002282"
     CDS             998..1231
                     /locus_tag="IXF88_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1508994.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            1319..1399
                     /locus_tag="IXF88_002283"
     tRNA            1319..1399
                     /locus_tag="IXF88_002283"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1353..1355,aa:Tyr,seq:gta)
     gene            1406..1477
                     /locus_tag="IXF88_002284"
     tRNA            1406..1477
                     /locus_tag="IXF88_002284"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1438..1440,aa:Gln,seq:ttg)
     gene            1992..2699
                     /locus_tag="IXF88_002285"
     CDS             1992..2699
                     /locus_tag="IXF88_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1508995.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(2758..3402)
                     /locus_tag="IXF88_002286"
     CDS             complement(2758..3402)
                     /locus_tag="IXF88_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1508996.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            3528..3815
                     /locus_tag="IXF88_002287"
     CDS             3528..3815
                     /locus_tag="IXF88_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1508997.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            3923..4243
                     /locus_tag="IXF88_002288"
     CDS             3923..4243
                     /locus_tag="IXF88_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1508998.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            4324..5637
                     /locus_tag="IXF88_002289"
     CDS             4324..5637
                     /locus_tag="IXF88_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1508999.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            5634..6353
                     /locus_tag="IXF88_002290"
     CDS             5634..6353
                     /locus_tag="IXF88_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1509000.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            6481..7032
                     /gene="raiA"
                     /locus_tag="IXF88_002291"
     CDS             6481..7032
                     /gene="raiA"
                     /locus_tag="IXF88_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1509001.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         2371 a         1191 c         1544 g         2066 t
ORIGIN      
        1 gaatattagt ttttgatttt ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag
       61 agattaaagg taagggacaa tttgcaacgc ctgaatcttg ggaagaaatg aaagcgcaat
      121 tatttcaagt ttataaaaaa atactcgttt cttttgaagt cgaaggaatt ggaatcagct
      181 ctcctggagt agtgaataat gagaaacagg tgattgaggg aatcagtgcg attccttata
      241 ttcatggatt taatattttc cgagatttgg aaactctgtt tcaacttcca gtaacgattg
      301 aaaatgacgc caactgtgcc ggaatggctg aattttatga aggtgctgcc cgcgattatc
      361 aagatgttgc ttttgttgtg gcaggaactg ggattggcgg ggcgttgttc acgaaaggac
      421 aaatcaataa aggggcacat ctttacggtg gagaatttgg gttgatgttc ttaaatggcg
      481 ataagacatt cagtaaactt ggtacagcgg tacagatggc ttggcgttat tgtgaacgca
      541 aaggtctcga taaaaatgct tataccggaa aagatgtctt tgaattagca gaaactggag
      601 atgcaatagc aaaagaagaa gttgagagtt tttatacgta tcttacaaag ggattattca
      661 gtattcaatt ttcgtttgat ccagaagtga tcgtattagg cggcggggtt tctgcaaaag
      721 aagggctcat tgatgagatc aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc
      781 atgatttcga accgcagatt ttattatgtg aatacagaaa cgatgctaat cttgtaggtg
      841 cagcagcaaa ctatctcgct gtacgtcaag gggagaatta agagctttag agaaggtgca
      901 gcttgcataa aagaagtgaa tgaaatgaat aggagatttt tacctaagga atgttttatt
      961 tgctgcagta tgataaaata tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat
     1021 tgaagtgcgt atagaagaag tgaaaaaaga tatcaaaggt aagaattatg aagtcactca
     1081 gttatttata gggaaaaaaa tgattggtga gatcttagcc tatggaccaa aagaatatga
     1141 aattttcttt ggtgaagaag attttggaaa agaaaaaagt cttgaaaatg cgatagaaac
     1201 agtgattcga cactggaatc tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg
     1261 gttgcaaaaa tctggaaaat taggtattat aactaagtca ggttgataca aacctttcgg
     1321 aggagtagcg aagtggctaa acgcgacgga ctgtaaatcc gttccttagg gttcagtggt
     1381 tcgaatccac tctcctccat ttcattgggg tatagccaag cggtaaggca agggactttg
     1441 actccctcat gcgttggttc gaatccagct accccagtat accacaatcg taagctctcg
     1501 ttagttcgag agctcttttt tttgctttgc taccttgttc cttgaagatt ctgctgattt
     1561 aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg aaagaaaaag
     1621 tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac aatattgaag
     1681 tgaatattcc gaaaaaaggc ggaaaagcgg tccgggtaaa atcaaaacgc ccaacgaatc
     1741 atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat aagtcggcat
     1801 tttcatcgat tttagggtct tttgttattt tctaggttgg acatatctaa aaaattgttt
     1861 ctgacaatag gagtaggaag aacgatgtgt gtctaaatcc tctgttgatg gatagaaacc
     1921 tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa acgaaaggac
     1981 gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac caattaaaaa
     2041 atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa agagaattag
     2101 cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac ttggcagatg
     2161 aaggaatctt agaaagaaag atcggttcgg gtacatatgt tgcacgagaa aaagtacaag
     2221 aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcacaaaat cgagtaccgt
     2281 ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg gaaaaactaa
     2341 acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca gatgatgtcc
     2401 ctatttgttt tgaagttgcc agtgtgccac aaaagctgat cgatggctac agcaaagcag
     2461 agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag atcggtccag
     2521 cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat ttagaggtaa
     2581 aacgaggaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac ggtacgccgt
     2641 ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg gaaaaataaa
     2701 aaacaggtga tccctgattc cgtattagaa ataaaagaga atcagaatca cctgttttta
     2761 tatatgatca gttaccagtt gctcgtgata agaaacagga ccctcttcaa attggacttg
     2821 tccgttcagc agctcaatga tggattcttt aaagtggtca gcactttttt catctacagc
     2881 acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat gttggagttc
     2941 aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga tctcctgttg
     3001 caaggtacct tctacgattc caatgtcaga caatgcttga gagaccgcat gagtgtatgc
     3061 tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga ctactgcagc
     3121 tacattgatc agttgatttt ttttgaggac ttccaacatc ggtatgcccg ccgttccact
     3181 tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga tgaaggcgct
     3241 gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaac tttttgcttc
     3301 gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga tctcgatctc
     3361 actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac ctcttttttc
     3421 attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa cggttgcgca
     3481 attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg caggaacgaa
     3541 tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct caagtaacgt
     3601 tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc aaaacagcaa
     3661 gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt gcctatggct
     3721 ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat gtcgtgttca
     3781 ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag aaaacgttga
     3841 caaatttctt tctttttata taatagagta acagacgcga agaaaacgtt tgcgtagttt
     3901 gttttaaaaa ggagagaaaa gtatggctaa aaaaacaatc atgcttgtat gttcggctgg
     3961 tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa aaggaatcga
     4021 ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa ataaagacgt
     4081 ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg agaaacgtct
     4141 ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta tgatgaatgg
     4201 cgaaaaagtg ttagatcaag caatcacgct cataggtgaa taaatagaac agtataacta
     4261 tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt tagggcgaat
     4321 aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc aatagactgt
     4381 gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt gagcaactgg
     4441 attctttgtc atcgctgtgg aatgagaacg tccgtcattg atggacagct tgcagattac
     4501 ggttattttt gtacacattg tctatattta gggcgatgcg acactcaaca agatttatat
     4561 ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac aggacagctt
     4621 actgaaaaac aaacggaaat ctcaaagaga atactttgcc attcagaaaa aagacatcat
     4681 cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat tttagtggaa
     4741 actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt ttgtaatgaa
     4801 ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct ccatgggaat
     4861 acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca gctataccgt
     4921 ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc ttataacggg
     4981 gatgcaggat tagctcatgc tgtgcaaaca gcgattaaac cagacggacg attcatctac
     5041 ttaagtgcca cacctgataa aaagttacta aaggagatca agcagacgtt tgacatccac
     5101 aaacttgctt tacgctttca tcggcgtttg cttcctgaac ccgtcttggt tttcatgaat
     5161 aattggcgac aaaaatgcaa gaacaagaag aagataaaat ctttggtgcg gatcattcat
     5221 caattaatta aagaaaacca tatcctttta ttttgtccaa gtatcacgat gatgcatcgt
     5281 ttgaaagcca cacttcaaga ggctcttccg agatatgaga tcacagatgt ttcttcaaga
     5341 gacaatcagc gggcggaaaa agtccagaaa atgcgagaag caaaatatga catactgtta
     5401 accacgatga tccttgaacg tggagtcaca tttgaaagaa tatccgttat cgttttagga
     5461 gcggatcatc cggtttttac taaatcttct ttggtacaaa tcgcaggaag agccgatcgt
     5521 aaagggcctt atacgaacag tcaagtatat tttttctatg aagaaaaaac tgcagctatt
     5581 cgaaaagctt gtcaggaaat caaagaaatg aatgaagaag ggaagaagat actgtgaggt
     5641 gcagttgctg cagtaaagat atcgtccaaa atttaacatt cagcgagatt attggtgttt
     5701 cgaaatacag attacctcag acttgttcag tttgtcgcgc acaatttcat ttattggaaa
     5761 caagaggctg ttctggctgt caaaaaccaa tccatttcaa tctagagagt acgaaaaaga
     5821 aagtaaattc tatccccgtt tactgcagtg actgtcaaga atggaagaag aaatatccga
     5881 cctatgattt tcagcaccgt gccttttttg tgtacgatga aaatatgaag gcctggctca
     5941 atcaatacaa gtttatggga gatatccgac tagcaggcac ctttgcatat aaatggacgc
     6001 agataaaaaa agaatacccg acatacatct attgtccaat acctttatct gaaaaaaggc
     6061 gaacagaaag aggattcaat caagtaagcg agatgttgaa agcagcaaga attccttttc
     6121 agatgctact caaaagagag aagcatctgc tggctcaagc aaaaaacacg cgaaaggaaa
     6181 gattggagat gcctcaacct tttgaacttg cagtcacctt ttctaagata caaaataaaa
     6241 aaatattgat tattgatgat gtctatacaa caggacagac tcttttccac gcagcagatt
     6301 gtttgcagat cgcacagccg aaaatcatcc gaactatttc tttggcgaga tgaattagat
     6361 gtggaaattt gatagcgatt taatttgcaa ataacctaga gttcgatata atagaattaa
     6421 gaagagggaa agagtggtcc ttctccttct tgattgtggt agacgaaagg ggtaattttt
     6481 atgtttagat ataatgtacg cggcgaaaat atcgaagtaa ctgaagcgat tcgcgactac
     6541 gttgagaaaa aagtaggcaa actagaacgc tatttcagtg acgcacccga tgcaacggcg
     6601 catgtgaact taaaagttta tacagaaaaa acagcaaaag tggaagtaac gatcccatta
     6661 ccttatcttg tattacgtgc cgaagaaact tctccagatt tatacgcaag tatcgatttg
     6721 gttgttgaca aattggaacg ccaaatccgc aaattcaaaa caaaaattaa tcgtaaatcc
     6781 cgtgaaacgg gcatgaatac tgcaaatgct gctgctttat tcaatgaaga ggacgaaaac
     6841 gaaaatgatt ctgaattaga tatcgtacgt acgaaacgcc tttcattgaa accaatggat
     6901 agtgaagaag cagtcttgca aatgaacatg ctgggacata acttctttat ttttgaagat
     6961 gcagaaacaa atggtacaag tatcgtttac cgccgtaaag atggaaaata cggactgatt
     7021 gaaacagact gatcagcctt acttaaataa caacgaaaac ttgcagcagc tactgacagc
     7081 tgccgcaagt ttttttgagt ttcctcctct tttagatgaa atatgaataa attatgaagt
     7141 tcttacttta atacgcttaa gatatttcaa tt
//
LOCUS       DACESX010000127         7172 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.127, whole genome shotgun sequence.
ACCESSION   DACESX010000127 DACESX010000000
VERSION     DACESX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.127"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..303
                     /locus_tag="IXF88_002292"
     CDS             <1..303
                     /locus_tag="IXF88_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1509002.1"
                     /translation="LGVKGQNLLADDSFWQIEAFYLAQCAYNTTLMLSPDRIIFGGGV
                     MKQEHMKKKVQDKFVELINGYVEIPPIASYIVTPELGDSAGIIGGLALARKAIRNK"
     gene            complement(1794..2459)
                     /locus_tag="IXF88_002293"
     CDS             complement(1794..2459)
                     /locus_tag="IXF88_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1509003.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2585..4621
                     /locus_tag="IXF88_002294"
     CDS             2585..4621
                     /locus_tag="IXF88_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1509004.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4625..5263
                     /locus_tag="IXF88_002295"
     CDS             4625..5263
                     /locus_tag="IXF88_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1509005.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5291..5719
                     /locus_tag="IXF88_002296"
     CDS             5291..5719
                     /locus_tag="IXF88_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509006.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6176..7087
                     /locus_tag="IXF88_002297"
     CDS             6176..7087
                     /locus_tag="IXF88_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1509007.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2460 a         1087 c         1331 g         2294 t
ORIGIN      
        1 ttgggtgtaa aaggccagaa tttattggct gacgattcgt tttggcagat tgaagccttt
       61 taccttgccc aatgtgcata caatacaaca ctgatgcttt cgccagatag aattattttt
      121 ggcggtggtg taatgaaaca agaacacatg aagaaaaaag ttcaggataa atttgttgag
      181 cttataaatg gatatgttga aataccacca attgctagct acattgttac gcccgaatta
      241 ggtgatagtg ctggaataat cggcggattg gcattggcta gaaaggcgat aagaaataag
      301 taattttaat gaataaatgt ttcattggta ctcatgtcaa aagtatttaa ctgagtaatc
      361 aaatgatgtt tgataaagcg aaaatgaatt caaataaaaa aggatcgagc aaactggaac
      421 atttgtccga tcctttttca cattctattt atttcgttta ttcaaccaat cggtagagac
      481 atccattgcg ctcttgtcgc gtggctttga tccatataat tgaccattac gaaatcatgg
      541 atcatcgctt tgaatgcgaa cttgtgtcac atctactttt ttgaccagat tttgaccagt
      601 ctattttaca ttctaatgta cttatatgag ttaatttttt gagtaaatgg taagtttttc
      661 cttgtttgtt ttatcttaaa ctagtgtgta atttgaaaat ctatatggaa gggtctgtgg
      721 ggtaatactt aaataaaggc taggaacctg ataacatcaa ggttcctagc ctttttataa
      781 taaaatgaag cgaaaataat ttcttttaaa gagccgagat tatcagctat ataagagtga
      841 atttgatgca aaaaacagag ccgaaacagt cttggtgttc gtagctctgc ttatatgttt
      901 cgaatttaag acaaagagtt ggcaaaagat gttgataagg aagataagat aactgtgtta
      961 tataaactag gtaactaact gtaacaagct aattttaaga gattcttatt agaagacgtt
     1021 taggaatata actaagaaaa tgattataca ggtaattgtc atttcaataa tgttttctaa
     1081 ttttcgcgtc tttgttttaa gaaaaaggcc agaatcagtt ggaaaaaagt tcgtacgata
     1141 gtgaagaggg gcaaaatttc ctactaatat tcccactaac aagagataaa acggccaggt
     1201 tggtgtgatt cctctataaa aaaatgccca tgctaccgaa atcccataga ttaacaactg
     1261 aacaattttt acaatgaaaa aattgttctg ccattactca aagtctcttc ctccttgtat
     1321 cgtttatttg tttcatttta tcattatgat aggcgcttga acaatccatt ttgtatttta
     1381 ttgagtactc ttggataaac taccagcgat tatcatattt atttatgtta ctgtaagcca
     1441 tcatataaaa gtaaatgagc agatcataga aatataaaaa agaactaaaa ttgatctaaa
     1501 ttatgtgcag cttgactacg taccaatcta tattacttac tagattggtc gttaatctat
     1561 tcggatcgaa tgagcaaaat atttttgtga atgtccatcc ttgattatga ttccaaaaag
     1621 aatcattgga aggctgtggc gtaaatatta tactataagc cagaaatacc tatcattctg
     1681 aactttttta tttaggaact ttcatctcaa ggctttttca attaatttgt acccctgttt
     1741 tttggataag atcatgtgca ttgatttttt ctatctctac gttcatctcc tacttaatgc
     1801 ctatctggct cctgagggct tctttttaag gcgtcgtcat agtcttggct gaggatttct
     1861 tggtaatctg agggaatggc ttcccaagtg agcaggcgaa cgttcatgac gtgttttcct
     1921 ttgtgtttga ttacagcatg gtttttctct ttttctacct ctccagcgtg aaaagacagc
     1981 catgtaccat ggccaaagat atcattacat acccaacgtt cgatggaatt gtccacgtat
     2041 cctacaggga gtgtggtata gccgatttgt ggtttcaaaa aaggattgaa tcgatcgatc
     2101 gtttcatttt tgtattgata attgataaga aaaagataga ccaaacaaaa gacgagcaga
     2161 aagaaagata gtgtcttttt gagtgagaag ggttgttttg atgaataaga tttcttgtta
     2221 acaagaggtt catctggcaa tgttggttct attgattcgg tagctgattc aatagaagga
     2281 aaatcaatcg gcagacctaa agtatgatgt aaaagtgaca gcatttcaaa gtctggcatt
     2341 gtacgagaat tttcccaact agaaatcgtt ttatcggaaa caccaatttt ttcgcctaat
     2401 tctttttgcg taagcttaca atttctgcgg taagttcgta ttttttctcc ggtgttcatc
     2461 ttcttctcct ttcgattcta cagtatatag atttattcta catgacctat acacttgata
     2521 gatacgtttt tttgatgaaa aacggtatga ttttatcaaa gaatcattta agtaagggag
     2581 agtggtgata atcaaaaaaa agttcacagt tttactcaca atcatagtga ccggtatcat
     2641 gagtggtttt gccttggtta gtataaataa acaggtactc tatcggacaa atgaattttt
     2701 ccatcaaaca gatacttata ctccgattcg attgccacaa cattttattg ggcaagaaga
     2761 atatgagcag ctaatcatgg tatttgaaaa tgtctcatca gaaactgagc tttcttatat
     2821 gaaaaaaaca gtaaatgaat ccaatgaagt agatacatct tggaacttca agatacagcc
     2881 aaagcttgaa atgacattgt attatcaagg ggatgtcaaa tcttctcacg agaaggtacc
     2941 ctattctaat acctcctttc gcgtaacgtc cattgaagaa tcgatcaaaa taaaagattt
     3001 tgaaggtgaa tttttcttga tgacgagaga aaaagaagcc tatgaacggg tcatttcact
     3061 attcactgat cgattcaatc aagcatttca cactgattat gccactaata tttttgaaga
     3121 ctttgaaaat agctattcgt attcggttat ttcaaataca gacgtatata attatcgagt
     3181 gtatttaact attggattga ttcttttatt ccttttgtta tcatcctttt tcttattgat
     3241 gaataaagaa atcagtttat tgttgaataa tgacttttca ttacatcaag tattgtggtg
     3301 tttattgggg aaaaagaaaa tcattattac attcataatt gcaggaatca ttggtggttt
     3361 tatttttatg tattataagg agcaatttct ttatttgttc cagatgtacg ttttcttgtt
     3421 cagcggaata tatggtatct ctttgatttc tcttttatta gttgaaaaaa tcgcttcgtt
     3481 aagagaagcg ttcaactata tccaatacat tctttttaca tttggattgt tgttttctct
     3541 tcttttgacc gttgccaatg tcgaattagc aactatcgtt ttttcttctt cacagttagc
     3601 tgtttcccat gaaccaccaa aagaaatcgc tggtgaggac tatcatgtat tttatccagt
     3661 aaccatcggg aaaaaccacg tggattttat ttattccaat cgttttgcat cagcagcaga
     3721 tcaggaatta tatgaaactt tgaataaaaa cggaagtata ttagtcaatg tatcagatta
     3781 cttgaatgaa gaaaatgaac agtttggcag agggataaaa ataaatctga attatcttaa
     3841 gaaatttcct ttgattgatg tctctggaag gttgatccaa ataaccgaaa aagaaacgca
     3901 ccctgttata ttgatccctg aacgatatag acatacagat ttaaaaaatg acctggcgca
     3961 aataacagaa tattatcaag aaatcagtga aaaagaaccg aaatttctat ttataaaaaa
     4021 caatcaaccc atttatacat ttattccttc tatcccctgg atcgaacatt atccggttgt
     4081 tgaaatactg acattgaaaa acagtatgta tttggaaaga aatattctca gcggagaaat
     4141 ctatccaccg ttaaagatca agataggctc tttgtcgaaa gaaaggttat ttgagctgat
     4201 tgaagaaagt cagttacgtg ataacttgca gttatcattt ccgtatactc agactgaaga
     4261 gatagctgtc aaagtgttaa gcagatcatt tcattacttg atacagatat ttttactcac
     4321 tttcttttct ttcttcttgt tgagctatgt catgttatgc atatattttg tctacaattg
     4381 tcggaaaatc gctatctttc gttcaagtgg ttattcgctc tttgaaacgt ataaagattt
     4441 cttcatgatg aacctcatca aatggggaac gacaagtgta atcttccttt ttcttataga
     4501 aagagagcct aaatatcttt ttaatatttt tttctttagc ttcattgatt ttattctttc
     4561 agttgttttt attttgtcta cagaaaagaa aagtcagtta ttgctgatga atggaggtta
     4621 acaattggag ccgttgatag tgataaaagg tgcgaaaaaa atcattcgaa acaaaacgat
     4681 tcttcatttg gatcgtttca ctgcggatgc tggggatctt gtgactgttg ttggagaaag
     4741 tggagcaggt aaaagtacgt tattgaatat attaggattg aatgatcagt tcagttcagg
     4801 acaattctat ttgttcgatc aagagacgaa acaattaact gggagagaga agcgcttgat
     4861 aaaaagaaat accatatctt ttctttttca agattttggg ttgattgaat atgaaacaat
     4921 cgattttaat ttagatattg gattgaagtt ttcgaattta aatgtttcag ataagcgaac
     4981 agcaaagtat gaggcattga aaaaagtaaa tataaacaag aagctaagta cgcctatcta
     5041 cacattatct ggtggtgaga agcaaagaat cgcctgtgcg agagtcttat tgaagaatag
     5101 caaaatcatt ttagccgatg aaccgactgg gtcattggat aaaagaaata aggataaagt
     5161 agttgaaatc ttatcgcaac aagcttccca aggtacgtta gttatcgtag tcacgcatga
     5221 tccatatctg accaaaatag gaaacaagaa gatcatgctt taaaaatcaa aaaggaaagg
     5281 atgtcgtttt atgaaaaaag cagttattat ttgtgcacta gggataggtt ttgttggagg
     5341 aaacgctatg tcatctgaag tggaagcaaa ttcagttcac ggtgaaatct attacaaatc
     5401 tttaaagcca ggagagattc attttgatgc taaaggtaat attattaagt caaatttacc
     5461 agtagaccgg gaaatttcta gaacagcaat taatcatagc tctggaagat gggtttatta
     5521 tagtgataca acttgtacga gaaaaacagg tcattctaac cactatagtc gtgtctataa
     5581 tcgtcacggt gcaaaagcga aagttggaaa ctctattcga tcagcaaatg caacaaaagg
     5641 agtatgggcc tatgcaagtg cgacaggaaa tagaactgac acattctcat gttggtacaa
     5701 tccaaccggt tggtattaag tatccaaaag tttatataaa atagaaaaaa catataatga
     5761 aacagcgtta aaagttattt ttggtagttt taatatattc atttttagtt gtataaaaat
     5821 atatcataat aaaaacaaca aaaaaatgtc tggaaaatcg aacataaaag tgtctgattt
     5881 gaatatccca gcagcggaag aagaaaaaca aaatcgataa catttgttct aagaattagt
     5941 tacgttaaat acggaacgca agatagactt gaaaaacggt aacaaaagaa gtagtatatt
     6001 aaggtgtgat gtgctacttg acatttattc tgctgagcga actttctgga ttgtgtagcc
     6061 ttatatctat tcaggacgta tcgagcagat tagtaaaggt gttcttgatg attttcatct
     6121 tgaacacctt tgtttttagc aattcgcaaa aaacgaaaag taggagaatt gataaatgaa
     6181 aaaaagattt ttagcacttg ccatcgtact aggcactggc ttattgtcag gctgtacgaa
     6241 tgccggtgaa aaaacagccg ttagttataa aggcggtaca atcagcgaac aagaagtaat
     6301 ggatagcttg aaaaaaatgc aaggagcaga ttccgctgtg caacagttga tcgtttatca
     6361 agtatttgaa gataaatatg gagatgacgt atcaaccaaa gaaatcgatt ctcaatatga
     6421 tcaaacaaag aaacaattgg gtgattcatt tgattcacaa ttgaaatctg caggttacac
     6481 agaacaaaca ttcaaagaca gtatcaaaca aagtctagcc tttcaagaag gtctaaagaa
     6541 acatatcaaa ttgaccgatg aagatttaaa aacggcttgg gaatctttcc atccagaagt
     6601 ggaagctcaa attatccaag tagcaagtga agatgatgca aaagatgtga agaaagctgc
     6661 tgataaggga gacgatttct ctaaattagc caaagacaag tcaactgata ctacgacaaa
     6721 agaagatggc ggaaaagtga aatttgattc cactactaca acggttccgg cagaagtcaa
     6781 agaagcagcc tttaagttga aagatggtca agtttctgat gtgatcactt ctaccaatgc
     6841 ttcaacttat actacggaat attatgtggt taaaatggtg aaaaaccaaa acaaaggcaa
     6901 cgacatggat aaatacaaaa aagaattgaa agaaattgca acagacacaa aattaagtga
     6961 cagtaccttc cagaataaag taattggcga agtattgaaa gacgctaatg taaaaatcaa
     7021 agacaaagac tttgaaaatg ttttgagtac attcacttca gattctagta cagcaagtag
     7081 taaataagcg gtataaataa aaaatatgaa ctatttaaat caataaaact gttcatctaa
     7141 atctttttat tagaactaaa tcaacagtag tt
//
LOCUS       DACESX010000128         7097 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.128, whole genome shotgun sequence.
ACCESSION   DACESX010000128 DACESX010000000
VERSION     DACESX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.128"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            234..707
                     /locus_tag="IXF88_002298"
     CDS             234..707
                     /locus_tag="IXF88_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1509008.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            704..2836
                     /gene="feoB"
                     /locus_tag="IXF88_002299"
     CDS             704..2836
                     /gene="feoB"
                     /locus_tag="IXF88_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1509009.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2838..2972
                     /locus_tag="IXF88_002300"
     CDS             2838..2972
                     /locus_tag="IXF88_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1509010.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3066..3710
                     /locus_tag="IXF88_002301"
     CDS             3066..3710
                     /locus_tag="IXF88_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1509011.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3897..5090
                     /locus_tag="IXF88_002302"
     CDS             3897..5090
                     /locus_tag="IXF88_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1509012.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5083..6810
                     /locus_tag="IXF88_002303"
     CDS             5083..6810
                     /locus_tag="IXF88_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1509013.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2302 a         1239 c         1573 g         1983 t
ORIGIN      
        1 gcaaatttaa tagagaaaaa attgtggaaa actatgaaga aatatttttt tctcattttt
       61 atctatcaat actaaagatt cgctcataat atcacaagta taattgacat tctcaattac
      121 cgagagtatt ctatgtgaga atgattctca tttatttgcg taacacgtaa gaaaattaga
      181 acatataatg aatgatttta atacatgatt tatttcacaa gagaggaatc aatatgcaga
      241 ccttagcgca ggcaaagcag aatttcgttt atcaagtcga acgaatcgaa acagaagaaa
      301 aaacaaaacg ccatttgcaa aatatcggga tcgttgccgg aagtaaaatc gtcgtcgtca
      361 atttatcagg caacaatgcc attttactgg taaaaagtca tcgagtggca gtaagtcggg
      421 aaatactagg tcaaatcttt gtcaataaaa agactgcttc ctctgaaagc tggacttctc
      481 ttgatcaatt aaaagcagga gagactggga tcgtcgtagc aatccacgga caaggcgcag
      541 tgaagcgacg gttaatggat atgggattga cgcgaaatgt aaaaatagtt atccgaaaac
      601 tagcccctct gggagatcca atcgaactga cagtgcgagg gtatgaactg acgttacgaa
      661 agtcagaggc cgaactaatc ttgatacaaa gggaggaaaa agaatgaata tcgcgttagt
      721 cggaaatcca aacagcggga aaactagctt gttcaatctc ttgactggtt cgaatcaata
      781 tgtagggaat tggccgggcg taacagtaga aaaaaaagaa ggatcgttga aaaaaaatcc
      841 gaacattcat attcaggacc tacccggcat ctattctttg tctccttata caccagaaga
      901 agtgattgca agagattatt tggttcagga acagcctgaa ctgatcatca acattattga
      961 tgctaccaat ttagaacgaa atctttattt aactacgcag ttattggatt tagacattcc
     1021 tatgatcatt ggtctgaata tgatggatgt catcaaaaaa gaaggaaaaa aaatcaatct
     1081 agaaaagctt tcctatggtc ttggtgtacc agttttacca atcagcgtat tgaaaaagca
     1141 aggaatcgaa aaactaatcg aaaagattca ggagtctgca aaaaagcggc cttctattcc
     1201 tgttccctgt acatatgatc cacgtttaga agcagcttta catgaaattg aagatgtact
     1261 aggagaacaa agcggcaaga aacgctggta tgcaatgaaa tattttgaac gagatcagaa
     1321 ggtaagagag gattacgaga tagcagtttc tcagcaaaaa gagatcgagc aactgatcca
     1381 gttaacagag aaactattgg acgacgatag tgagacgatt ctggtcaatg aacgttatga
     1441 atttattacc caactatgta cactctgtgt tgtgtcaaat gattctttcc aattatcgat
     1501 gagtgacaaa atcgatcaga ttgcaacgaa tcgttggtta gccttgccga tcttcgcatt
     1561 tgtgatgtgg ctgatctatt atctggctat tcaaacagta ggaacaatgg ggacagattg
     1621 gatcaatgac acattgtttg gtacatggtt accagaacat gttggcagat tgttagctga
     1681 atggcaagta gcgggatgga tgcaggagct gatcttgaac ggcatcatcg caggcgtggg
     1741 agcagtgtta ggatttctac cgcaattgat tatattgttt ttatgcttga gctttctgga
     1801 agactgcgga tatatggcga ggatcgcttt tgtcatggat cgacttttca ggaaattcgg
     1861 tctttcagga aaatccttca ttcctatgct gattgctaca ggatgtggag tgccaggcgt
     1921 catggccagt cgcacgattg aaaacgagca agatcgtcgt ttgacgatca tggtcacaac
     1981 cttcatgcct tgttctgcaa agttgccgat tattgcctta gtagcagggg ccttttttcc
     2041 tcatcaatct tgggtagctc cttctgccta ttttcttggt atgactgcaa ttattttctc
     2101 tggaatcagc ttgaaaaaaa cacgtttgtt tgctggagat acagctcctt ttatcatgga
     2161 attgcccgca tatcattttc ctcaatggtc agcggtccta agacaaacct atgaccgcag
     2221 caagtctttt gttaaaaaag caggaacgat tatctttgtt tctagtatcg tgatctggtt
     2281 cagctcttct tataattttt atgctcaacc tgtttctgaa gaccaaagta tcttagcctt
     2341 tttcgggcga atcctggcag ttgtttttca gccgcttggt tggggaaact ggcaaggaac
     2401 agtcgcagcg attacaggat tggtcgctaa agaaaatatt attggcacat ttggtatttt
     2461 gtttggtcat gcggaagtat ccgaaaatgg cagagaaatc tggcaagtgc tacaacacac
     2521 atatactccc gcagcagctt attcattgtt agccttcaat ttactttgtg cgccttgttt
     2581 tgcggcaatt ggggctatcc atcgggaaat gaatgccaag aaatggactt ggatcgccat
     2641 aggctatcaa tgcggtttag cttatttagt tagttttgtg atttatcagt tgggacactt
     2701 attggagggt ggacaggtag cgttagggac gtatcttgct attttactga tgataggact
     2761 cgtttatgtt ttgttccgtc agccaaaatc caagaaccag ttttatacta tattgtcatt
     2821 agaaggagag gaataggatg gcgacattct tattaagtgt gttaatcttt ggaagtgcag
     2881 gcgcaattgt ctatactcgt ttaaaatccg gtaagaattg tgatgactgt caaacagctt
     2941 gcccagtaaa aaaggaacag atcaagcgat gatcagtcgt tgcaagaggc tattctcccc
     3001 ttttttgctt attttgctag agcatagtaa gatgagggaa agtaaaaaaa gaaagaggaa
     3061 agaaaatgtc tggaaagaaa aaaatcgtcc gcccgcgcta ccagcagatt gctgttgatt
     3121 tagccgaacg tatcgttgaa aaccgatata aagtgggtga gaagatccat gcccgctcaa
     3181 cacttgccag tacgtacaat gcttctcctg agactacccg caaggctatc aatgtgctgg
     3241 tagatcttgg aatcatggaa gtacggcatg gaagtggggc atttgttgct tcgaaggaaa
     3301 aagcaaaaga tttcctattg caatatcaag atgtacaatc catccaagat acaaaagcag
     3361 aaatcatggc aagtgtggag aagcaacaag atgagctgaa tcatttttct caattattgg
     3421 atcagctggt caatcaaacg aagcgaatac ataagatgaa tcctttactt ccgtttgaat
     3481 tatctttatc agaaaaagca atgcatctgg accgttcaat tagcgagttg aatatctggc
     3541 aggcgactgg cgccacagtt atcgcaattt ctcataatga agaactgctt gtttcacctg
     3601 gcccctatgc taaatttacg gcaggggaca tcgttcattt tattggaaat gaatatacac
     3661 tacagcgtat gacaaacttt ttttacccgg agcatcagct tgcagaatga ttgtgtgaaa
     3721 tattaaaaaa atattaactt ggctaaacct tttatttaca aagatttagc caagtttttt
     3781 tcagtttgga caagcaaatt tctttcattt ttgacaaagt gactacatga tgttaacata
     3841 gtgtggtcac ttttttcaga aagaaaaatg aatttacata aaaaaggaga atttgtttgg
     3901 taaaagtaga agtaaaacat ttaacaaaga tatttggtaa aaagacacaa gccgctttag
     3961 atatgatgaa tgatcaccag ccgaaaacgg agattttaaa gaaaaccggt gcaacggttg
     4021 gtgtgtatga cgtgaatttt gacgtaaaag aaggagaaat cttcgtgatc atggggcttt
     4081 ccgggagtgg gaaatccaca ttgatccgtc tattgaatcg tctgattgag ccaacttcgg
     4141 gcagtatcta tattgatgga gaagatgtat cgaagttaag caaagaagaa ttgcgagaag
     4201 tccgtcgtca taaaatcaat atggttttcc aaaacttcgg tctgtttcca catcgaacga
     4261 tcttggaaaa tacagaatac gggttagaag tccgtggtgt acctaaagaa gaacgacaag
     4321 aaaaagcaga aaaagcatta gaaaattcta gtttgctttc atttaaagac caatatccta
     4381 gtcagttatc tggtgggatg caacaacggg ttggcctagc cagagcttta gcaaatgatc
     4441 ctgagatctt gctgatggac gaagcgttct cggctcttga tccgctgatc cggcgagaaa
     4501 tgcaagacga attactggac ctgcaagcca atgttcaaaa aacgattatt ttcattaccc
     4561 atgatttgaa tgaagcttta cggattgggg acagaatcgc cttgatgaaa gacggcgaaa
     4621 tcatgcagat cggaacaggt gaagaaatat tgaccaatcc agcaaatgat tatgtacgtg
     4681 aatttgtgga agaagtcgat cgttccaaag tgcttacggc tcaaaacatc atggtacctg
     4741 cattgacgac gaatatcgaa tctgatggac caaacgttgc attgacaagg atgcgtaatg
     4801 aagaagtcag catgctgatg gctgttgaca gaaagagaca cctgaaaggg atcatcactg
     4861 cagatcaagc gttagaagct aggaagcaaa aacgtccatt gatcgacttc ctagatgaaa
     4921 atgtgacggt gattgggaaa gacatggtgg taagtgatat tttcaatatt atttatgact
     4981 caccgactcc acttgcagtt gtagaggatg gaaaattaaa aggtgtcgtc atccgaggta
     5041 gtgtgattga agcattagca gaaacaagcg aggtgagtga acatgaataa tctattgaac
     5101 tatcaattac cagtggcaaa ctgggtagaa aatatcacag aatggtttac gacaactttt
     5161 tctggcctat tttcattttt acaaacgatt ggacaggcag tgatgtcggg gattacaaat
     5221 ttactgttag tgattccagc tcctttgttt atcttgttat tgacggtagc agctttcttt
     5281 atttctaaga aacgtccagg acttacgttg ttcacattaa tcggtctatg gtttatttat
     5341 aaccaaggtc tatggaatga tttgatgaat actgtcacac ttgttctttt atctagtgtg
     5401 atctcgatca tcattggggt accattaggg atcttgatgg caaaaagcag taaagcccaa
     5461 agtattatca aaccgatcct tgactttatg cagacaatgc caggattcgt ttatttgatt
     5521 ccagcagttg catttttcgg tatcgggatg gttccagggg tatttgcctc agtcatcttt
     5581 gctctgccac caaccgttcg ttttacgaat ttagggattc gacaagttcc taaagaatta
     5641 gtagaagctt ctgattcatt cgggagcaca agtcgtcaaa aactattcaa actagaattg
     5701 cctttagcta aaagtacgat catggcaggg atcaatcaaa caacaatgct gtcactttca
     5761 atggtcgtta ttgcctctat gatcggtgca cccggcttag gacgcggcgt attatcagca
     5821 ttgcaaagag cacaagtcgg gaatggattt gtcaacgggg ttgcattagt tatcttagca
     5881 atcatcattg accgttttac acagcatttg aaccagccaa acaataaaaa agctgcagga
     5941 gcaacaacac agaaaagtaa aaaacgtcaa ggactgatca ttggtgctgt agtagtagtg
     6001 atcttaggtg cgattggtat agggagtttt tcatctgcca aagaaacaaa acgtatcaac
     6061 ttatcttatg ttgaatggga tacagaagtt gcttcaacaa atgtagtcgg tgaagtattg
     6121 aaacaaatgg gctatgatgt aacgatgacg ccattagata attcaatcat gtggaaatct
     6181 gtttcaaatg gcgagtcaga tgccatggtt tcagcttggc tacctaaaac tcatggttca
     6241 caatatgcac aatataaaga tcaagtcgaa gacctaggtg ccaatttgac aggagcaaaa
     6301 gttggtttag cagtgccagc ctacatggat gtaaattcga tcgatgagtt aaccgatcag
     6361 gcaggtaaga aaatcatagg aatcgagcca ggagctggtg tagttacggc tgctgaaaac
     6421 acgatccaaa aatatgacaa cttgaaagac tggaaagtag agacatcttc ttcaggagca
     6481 atgaccgttg cactaggtca agcgatcaaa aaacacgaac caattgtagt tacaggatgg
     6541 acacctcatt ggatgtttgc caaatatgat ctgaaatact tagaagatcc tgaaaacgga
     6601 atgggaagcg aagagcagat ccatacgatg gttcgaaaag gattgaaaga agaccagcca
     6661 gaagcttaca aagtattaga caatttccat tggtcagaaa aagatatgga aaaagttatg
     6721 ttggaaatca acaatggaaa agatccacag caagcagcaa aagactggat caaagagaac
     6781 caagaattag tcgaaagctg gaaaaaataa aaagacagac acgaaataaa tggagcttcc
     6841 aaaattggat caaatatcca aatttggaag ctccatgttc gtctctatct tttttgtcta
     6901 taaaaaaaga gaatgcgtag ctcatcttcg aaacgttcaa agacgccgac aaactattat
     6961 gaatttatgg gtgcgagtca aagagatcga tcgtaggtat gaaagactaa tgtgattaaa
     7021 gtttattatg tacgaagtgc attctcaaaa aatattaaag aatatacata gtttatatac
     7081 ttaaaagtga ttaaatt
//
LOCUS       DACESX010000129         6936 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.129, whole genome shotgun sequence.
ACCESSION   DACESX010000129 DACESX010000000
VERSION     DACESX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.129"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            172..1029
                     /locus_tag="IXF88_002304"
     CDS             172..1029
                     /locus_tag="IXF88_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1509014.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1022..1924
                     /locus_tag="IXF88_002305"
     CDS             1022..1924
                     /locus_tag="IXF88_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1509015.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1957..2307
                     /locus_tag="IXF88_002306"
     CDS             1957..2307
                     /locus_tag="IXF88_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1509016.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2341..3279
                     /locus_tag="IXF88_002307"
     CDS             2341..3279
                     /locus_tag="IXF88_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1509017.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3357..4250
                     /locus_tag="IXF88_002308"
     CDS             3357..4250
                     /locus_tag="IXF88_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1509018.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4288..4851
                     /gene="amaP"
                     /locus_tag="IXF88_002309"
     CDS             4288..4851
                     /gene="amaP"
                     /locus_tag="IXF88_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1509019.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4864..5052
                     /locus_tag="IXF88_002310"
     CDS             4864..5052
                     /locus_tag="IXF88_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1509020.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5065..5967
                     /locus_tag="IXF88_002311"
     CDS             5065..5967
                     /locus_tag="IXF88_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1509021.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            5999..6241
                     /locus_tag="IXF88_002312"
     CDS             5999..6241
                     /locus_tag="IXF88_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1509022.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6497..6868)
                     /locus_tag="IXF88_002313"
     CDS             complement(6497..6868)
                     /locus_tag="IXF88_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509023.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2528 a         1181 c         1385 g         1842 t
ORIGIN      
        1 tgaataaaaa gccatgaaaa acgaatagtt agcgcttacc gatgcgtttt gtgataaaaa
       61 aattttaaaa tattcaatga aaacaaagca tatattttgc tttaaaacca aatgaggaat
      121 attctagatg tagaacaggt tagaaaaaga ttagaaaaag gagtgatttt gatggagaag
      181 agatacggcg tagttcctgt ggtaactgct tcagacgaaa attatgctcc ttacttgagc
      241 gtgatgatcg caactgcatt agaaaattgc aataaaacaa gacgcataaa attttatgtg
      301 attgacgatg gtctatcaga atatagtaaa gaaggattgg aagaaacggt aaataaatat
      361 tcaagtaatg caagtatcca attcctgaca gtggaaaaag atatctacga agacttccta
      421 gtcagtgacc atatcacaac taccgcttac ttaagaattt ctttaccaaa cttattagct
      481 aaagaggatt ataagaaagt cctttactta gattcagatg tgttagtact agacgatatc
      541 gtaaaactat atgatgagcc gttaaatgga aaaacaatcg gcgctattat cgatccaggc
      601 caagtgaaag ctttggaaag attaggaatc gattccgatg acttgtactt caattcagga
      661 gtgatggtga ttgacattga tcaatggaat aaaaaagaaa ttacagaaaa aactatccat
      721 tacctcagtg aaaatggcga tcgaatcatc tatcacgacc aagatgcttt gaatgctgta
      781 ctttatgaag attgggaaca acttcatcca aaatggaaca tgcagacttc gctgattttt
      841 gaacgtcatc cagcaccaaa tgaaaaatat gaacgtcagt ataaagaagg aaatgaaaaa
      901 ccttcgatcg ttcactttac aggacatgat aaaccttgga atacgttgaa agatcatccg
      961 tataccaatt tatacttaaa aaaattagca cacagtactc taacgaaagt aggcgagatc
     1021 aatgaataaa aaagaaatag cagtagtcgc aagttgtaac acaaaattcg taccacattt
     1081 agctgcatta tttgtctcag tattagataa ttgcaacccg tctaaatttg ttcggttcta
     1141 tgtgattgac gatgatatcg attttgaaag taaacagctt ttacgcttct cagtgaaaaa
     1201 cgcacgaatg aactcagatg tggaattctt gaaaatcaat aaagaattct ttacaaatgt
     1261 ggtaatcagc gatcgtattc cagaaacagc ttattatcgt attgcgattc cggaattgtt
     1321 ccgcggcaca gaagttgaac gtatcttata tatggattgc gacatgatcg ctttacaaga
     1381 tatctctaaa ttatggcgtc ttgacttcgg tgattcaatc gtagcagctg ttgaagacgc
     1441 tgggttccat caacgactgg aaaaaatgaa aatttcagct aaatcaatgc gttatttcaa
     1501 ctcaggtttg atgctgatca atgtgaaaaa atggttagat gaaaatatta cgcaaaaagt
     1561 attagatttc atcgaacata atccagaaaa attacgattc catgaccaag atgcattaaa
     1621 tgctatctta catgaccgtt ggttaccact tcatccgcgt tggaatgcac aaggctatat
     1681 catggcaaaa gcaaaaaaac atccaacagc agcaggtgaa cgtgaatacg aagaaactcg
     1741 caacaatcct tacattatcc atttttcagg acatgtgaaa ccgtggagca aggattttga
     1801 agggccaacg aagaaatatt acgaaaaata tgcagggatg acggctttcc gttgtgtagc
     1861 taaattccct aaatatccaa aatatgcaaa agttcaacca aaaacagatg tagttgttaa
     1921 ataatagaag acaacaactg aagaggggga gtaggaatgg aaattacaat gtctaaaaat
     1981 gcagtagaga cgttaattga aaaggttggg gaaaatacaa aaatcgcact cgcgcttatc
     2041 aatgattccg atccatttct aagggacaaa ggtgcttttg caaaaggcag cttttttcaa
     2101 atcattcctt ttgtttcgga atttggagaa tatgcgacta aaatcgagca tccattattg
     2161 gacatctata catctaaatt agagcaaaat tatttcggta aacgattgaa catggacttc
     2221 aacaaacagc ttgacagctt ttcattagaa aatgaaatcg cagtactcga ttataatatc
     2281 aaattaaaga actgcttctt cagctaatag tttcgagaaa gtccaaaaag gaggtataca
     2341 ttgagatact actttgtgga tgaagaaaag aatgtaatag aaacagatga aaaaaaatat
     2401 aattggctcg tacttgatag tgcaaagccc gaagagattg agcaagcaat cgaagaattc
     2461 gagcttcctg atgatatttt cgtaggaacc gaatatccgg aagaagtttc tcggatggag
     2521 cgcctttatg agacgaaact agagcatcca ttttctttgg tagtgattaa tctagatcag
     2581 tcagataatc gtatcgaaaa aaaattaacg ccaatatctt ttgtactttc cgataatctc
     2641 ttgattactt gtatcgatag aaaaaccgaa tttgtggatc ggctgctgac aaagcatgga
     2701 aatcatttaa actcgtttga aaagatcatc acgtatacat tattagatat ttacacccat
     2761 tacgtgaaag aactaagaga gatgaaaaag cgaattgacg ctttggataa agcagctcgt
     2821 aaaacaacgg aaaatgaaga actgtataaa caggcagatc ttgaaagaga catcgtatat
     2881 atcgatcata ccttaagaga tcaacgggaa acaatggatc aattatggaa ttctgaaact
     2941 ttcatcaaac gtttagacga tgaacagcta ctctatgatg tccatttgag acagcgacaa
     3001 acagaaaaaa tgatcgagat ctatcgagat ttattagaga gtattggtga tctattcaac
     3061 gggatgatgg acaataatct aaatcatatc atgaaatatc tagattcagc tgcattaatc
     3121 atttccgttc cggcgttgtt cgcagggatt tggggaatga atacaggagg attaccgggt
     3181 aaaagttctg ctctaggctt tatactcgta gtctttggct ctttgatagc agcagtagtt
     3241 ttaggttacc atttataccg gaaagattat acaaattaaa gaatctattt aaaacaggta
     3301 tcaaacaaag aaatttgttt tggcaataca atttaaaaac taggaggaat acagcaatga
     3361 cagaaccaaa attaatcgac ccgcgaaact tgtaccatac ggagaaattt cctaaacaag
     3421 atcaggatac accagcttta cagcagaata tgattcctgt tcctgattgt ggagaagaat
     3481 cctatgaagg caataatcaa ctagaaaacc gccgtgtact aatcactggt ggagattcag
     3541 ggatcggtcg tgcagcagcg attgcatttg ctagagaagg ggcagatatc gctcttcaat
     3601 tcttcccagg agaagaaaaa gatgcagagg aagtagcaaa atacgtgaaa gatgcaggac
     3661 gtaagattgt cttactacct tttgatcttc gtgacgaaca agcacctaaa gaaatcatag
     3721 ataaagccgt gagtgaactt ggcgggctag atacgatggt actaaacgca ggacagcaaa
     3781 tttcttgtca gtctattgct gagttaccac ttgagcaagt caaagacaca tttatgataa
     3841 acatcatcgc tatgtttgct ttagtgaaag aagctgttcc gcatatccca gcaggaggag
     3901 caatcgtaac aacaacttct gttcaagcat tcaatccaag cgaacattta ttagattacg
     3961 cagcaacaaa agcttcaatc gctaacttta cgatcggttt agcaaaacag cttgcaccta
     4021 aaggaatccg tgtaaatggt gtagcacctg gtccaatctg gacaccatta caactagacg
     4081 aaggacaacc ttctgattcg atccctgaat ttggtcagca aagtttacta gaacgtgcag
     4141 gtcagccagg agaattagca ccagtttatg ttttcttagc atcgaataaa gcaagttatg
     4201 ttacagccca aatttacgga gtaactggtg gagaagcgat caacttataa gaagttgatc
     4261 gtttcacccc tatgaggagg tgcgaaaatg aataaaggtg taaaaacgat cggagtgatc
     4321 ggaacattac ttttgctatc tgtgcttttg tttgcgatac tattaagtgc aacctatcca
     4381 ttgccttttc agctagagaa gtttcgattc ttttccatca ctaattcata cgtgcaacaa
     4441 tatgtatttt ggcttgccgt tgcatttgct attttgacaa ttatcacgtt acttgttttc
     4501 ctgttttatc caaaaacaat cggaaacttt ctactgaaac atggtgatgg ggagttgacg
     4561 ttggataaaa aagcaattga aggaatggtt cgttctcact tgcatgaaga tgaattcatc
     4621 cattcaccaa aagtaaatgt caaagcaacg aaaaacaaga tcgccgtcaa tgtaaaaggg
     4681 gagttgaaac gaacttcagc actcattggt aaaacaggaa cattgatgaa agaaatcgaa
     4741 aatgaagtac aacacatatt gggaacaaaa gagccagtga aagtatcggt gaaatacaca
     4801 ggctttgaag aaccagctaa acgaagcaat gaccgcaaac gagtagaata ggggggacca
     4861 aaaatgaaag aactattttc gacatacaag ctccctatca tctttggggt acttggattg
     4921 atattagccg ttttatggat caccgtgggc tttttcaaga ccttattgat cttgatcatg
     4981 gcagcgatcg gcgtcgctat cggtttttat ctgaaacaaa cgaaaatctt agatcactat
     5041 tttaaatctt aggaggaaac atccatgaat aacaataaca aaaataataa caataaaatg
     5101 aacaaccaaa aaccagtgtc tgcacatcca gacccaacga caccaaacca atcaaatggt
     5161 gtgaacgatg aaaagaacaa aacagcacca gtatctgcac atccagatcc agtagtccct
     5221 aacgttcaac aaggagtaga agagaagaaa catgatgcag aaaagcatgc acctcatcat
     5281 ccaggaactc cagaatctaa agttccagaa caacatgatg caatcgatcc ttctaaacct
     5341 gtggaagagc aaaaacatcc ttctgaccat gcgaaagcac aagaaaaact tagttctgac
     5401 cacacaaacg gacaaggaaa acttggttct gatcatacaa ataaccaagg aaagcatgat
     5461 tctgaacagg aagattcaat gcgcggtgag ctgaattatg aagataaagt tgtgcaaaaa
     5521 atcatcggaa tcgcaattga acaagtagac ggtttattat ctgcaaacgg tggtttcttc
     5581 tctaatgtgg caggaaaact tgtgaataca gataatgtaa cggctgggat cgaaacagaa
     5641 gtcggtaaaa aacaagtagc agttgatatg gatgtcattg tggaatatgg caaagatatc
     5701 gaaaaaatct ttgaagaaat gcaagaagta atcggcaaag aagtaaaaaa tatgacacat
     5761 ttagaagtga tcgaagtcaa cgctaacgta gtggacatca aaacaaaaga agaatttgaa
     5821 aaagatcaag aaacggtgca agacaaagta actgacgcag cgaaaaaaac cggtgaattt
     5881 gcttcgaatc aaacagataa agtaaaatca gcagctggaa gcggcgcaca aaaagtaaaa
     5941 gaaaatacag aaccacgggt tcagtaataa ggaagtaaaa cattaggagg aacgaaacat
     6001 gggatttatt tggtcactta tcgtcggagg aattatcgga gcaattgcag gagcaatcac
     6061 gaataaaggt tcttcgatgg ggattattgc aaatgtcgta gcaggtttag ttggatcagc
     6121 acttggtcaa gcattattag gttcttgggg accaagtatt gcaggaatgg caatcgtgcc
     6181 atcattgatt ggtgccatca ttttagtact aatcgtatca ttctttgctc gaaaactgta
     6241 aaaagacttt taaaaagagg tcgtgaaaaa gctgttcttc atcaagtaat aagaacagcc
     6301 aaacaaaaat agcttctcat atttttcatt tggttgggca tattatcgaa gatgcaagct
     6361 tttgaacact gtttatacgg aataagaggc tgtgacaaga cttttgtcac agcctcttat
     6421 ttatgcgggc aaattcccaa ttagattttt aaaagttttt ccttaaatgt gtctttattg
     6481 atacagagaa gcttctttaa taaaacaatg cctccctaaa ttttcttgag ataggaaagg
     6541 tgaacataca aaataatacg accactttga aaacccagat gatccacaaa tggatcgtat
     6601 gatactgatg aagtaaaaag caatggatca gaaactcaaa gagattgaaa aagacaagga
     6661 agaaataata tctctttttt actcttgtct gttttagcca ataatagctc gtaaagtaat
     6721 ctagacaaac aaatagaagt aaacaaagtt tcgtaagagc agcaatcgta tcatgttgct
     6781 ctgctagacg attgacgagc aggaaaaatg ttggtaatgg aatatagatc acgagtaatg
     6841 aaagaaaaaa gtaaacaata aatggcatac tattcctcct aattgtgtga gttcgccctc
     6901 attttaaaat aacgatattt gtttgtgaag cttttt
//
LOCUS       DACESX010000130         6889 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.130, whole genome shotgun sequence.
ACCESSION   DACESX010000130 DACESX010000000
VERSION     DACESX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.130"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            326..682
                     /locus_tag="IXF88_002314"
     CDS             326..682
                     /locus_tag="IXF88_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1509024.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            692..1591
                     /locus_tag="IXF88_002315"
     CDS             692..1591
                     /locus_tag="IXF88_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ1509025.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2108..2839
                     /locus_tag="IXF88_002316"
     CDS             2108..2839
                     /locus_tag="IXF88_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1509026.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2984..5011
                     /locus_tag="IXF88_002317"
     CDS             2984..5011
                     /locus_tag="IXF88_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ1509027.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            5119..6216
                     /locus_tag="IXF88_002318"
     CDS             5119..6216
                     /locus_tag="IXF88_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1509028.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6523..>6889
                     /locus_tag="IXF88_002319"
     CDS             6523..>6889
                     /locus_tag="IXF88_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1509029.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVS"
BASE COUNT         2410 a         1108 c         1397 g         1974 t
ORIGIN      
        1 gataagttta ctctaccgaa gagttgttaa taatccgtga acaaaaagta attctttttc
       61 caatagttgt taaaggtttt ttatttttta tagtattttt tgttatttaa gaaaggaata
      121 aggttaaaca aataacaggt tgctttttgg gtctttttcc gttcaagaaa ctgaacgaag
      181 aaaagggaaa gttgtctctt ttgtgataag agaaatcata ataaacgaat gacttacttt
      241 ccaataaatt gagaacagtc agaaccagac aaattagttg catctgatga ataaagtgat
      301 tattctatga catgaggtga tacagatgaa attagaagtg acaaaagaag cacaagaagt
      361 attgaagaat aaattagaag cagacgatca acttttatta gatatcgaaa acggtgacgg
      421 tccatttgca gaaagccaag tcagttgcca attggatacg gcatttcgat tgatcattgt
      481 caaaaaagat acacaaacag atctaagtct ttattctgta gtggctgata caccagttgg
      541 accaatcaaa atcaaagaca gtgcaatact ttacatggat gatccatcca cacttgcttt
      601 agaaccaact tataatagtt tacaattaaa aggaccaagc ggcttgagaa aaggcaactt
      661 gcaagttgtt cgaaaagact agagggataa aatgggaaaa ttggcgatca tcagcgacct
      721 acatgtggac atcaataaat tcggcgaatc agagctgatg cagctttggc aagttttgca
      781 aaagcacaag atcactcggc ttcatctagc cggagatacg gctaataaag tagaccgttg
      841 cctagctgtt gtcgaatttt tcgtcaataa aggattatct acgacatttc atttcggcaa
      901 tcacgagttg gcagatgtgc ccaatgaaca gatgatggac cattttccag acccacattt
      961 tttaaatgaa cggtatattg ctttaaatga gcagaccgtt cttttaggga tgaatggctg
     1021 gtacgactat cagttttccg aattgaaaga ttacgaggca atcgtacgaa tgaagcgttt
     1081 gtattggtat gatcggttca tccagcgtgg gaaaacagat ctcgaggtca atgcagaaat
     1141 gcttgcagct gcaaaaaatt tgctagacca gctggaagca aaaaaacttg aggtgatttt
     1201 atccactcat ttcgttccta aacgggcatt tatcatgtat caatctgctc cttatgagcg
     1261 ttggaacaaa ttaaatgcat ttttaggatc agaatcattc ggagaattac ttgaccgtta
     1321 ttcaaatatc aaacaagtag tttttggcca tacgcatcgt cgctttgaag ataaatccat
     1381 cggtcacacg atttacagct gcagaccttt tggttattat tatgaatggc agctgacccg
     1441 agattttgtc ttgaaagaac agttgctaga cagctatgat cctatgaaat tacgtgtcct
     1501 cttgcgtgcc catcacccag cttttcaggc ctatcaaaca caacatttgc aagaggaatt
     1561 tgaacaagcg atgacaatcg ttccgtattg agacagcatt agtaatgtgt aaaagaaaat
     1621 accttgtaaa acataaggat aagtaagagc agtagatcca tttatcgtgg atatgctgct
     1681 ttttttgcgg aaaataatca gcgaatgttt atttgatcgg atgatttgta actcgcagag
     1741 tactagatgc aagcgtgtat tctttttttg aacgataatc acaaaaaaaa atataatata
     1801 cattttttat ttaattataa ttaatattga ttgaagtcta ggcagtcaag tcacattttg
     1861 atagatagga gggggactta cgaattattt taaaggctat gacagctgta tttctagatt
     1921 gtgtttgcat caagttgcaa taaaaactag aaatcaaatc gggctgataa tcatttataa
     1981 ctaatcgata cataggacca aatataggtc tatgttcttt tatttcataa gtatcgtaaa
     2041 acagaatttt gctatggatt aatccaatcg actgatatgt ttagtaaata aagaaagagg
     2101 aatgtcaatg atgaaaaaag aaatcgtatt aacagcagca atgatgttat tcagcatggt
     2161 agcaagtacg acttttgttt ctgcaactga agtataccca aaggaatata acacagaagg
     2221 aacgattaca tttgaagcag gagatgaggg tgtaactcct cctgttgatc cagagaatcc
     2281 tgatcccaat aaacctgtcg atccatcaga tcctccaagc cctggaacag gaggggcatt
     2341 atcgattgat tatggatcaa aattcaaatt tgggactcaa aaaatttcta ctgcggataa
     2401 aacgtattat gcagctgctg atgtgatgaa cgatgggtct agaaaaccga catatgtcca
     2461 agtgacagat aggagaagta ccttatctgg gtggaaatta agtgtctctc aaccagaaca
     2521 gtttaaaact gccagtgggg atgaactagt tggagcacag ctgaaattta cgaaagggca
     2581 ggctgtttct ttggttgatc caacatatac gccgcaaacg gtgaattctg aattaacgtt
     2641 gacaccggga ggaaacaata cgttagcaat taatgctaaa tcaggaacag gtgtggggac
     2701 ttgggtctat cgatttggtg caaatgaaaa tgaaaatcaa gatgcggtac agctttcggt
     2761 acccggtaaa tctgtcaagt tagcgcaaca atattccact aaattagttt ggacattaga
     2821 agatacacct aacaactagt ttactgaaaa gaactgttaa aaataaatac gtagtcatat
     2881 tgtcttgcga ggtaatcgag caaaaattgt caaaggctag gctaaatttg tttggatacg
     2941 aatagttcat ctaggaattt aacgatcagg aggaaaagat atattgaaaa agaggaggat
     3001 cgtgctcatt ttaagcagtc tcttagtgac taatatcttt ccgatcatag ggtactcttt
     3061 ggaattgaat cagttggagc atggaaatag aagagacgaa gaaagcaaac tcacttcgac
     3121 attcgacagt ttaatagaat ccaaccagac acaagaaagc agtgaggatt taagtatttc
     3181 gagtagtgga gagaccactg acaacgtaga agctgagggt tcggacaaac aaggactaga
     3241 agatgcacca atttctgcgg ttgagctgcc tgacgatccc aatattattt ctatcgataa
     3301 aatttttcaa acacctatcg gtactagcac gtccatattg gaaaatggga agttactgca
     3361 gttgaaccca gcagaaaggt ctcaaagagg agctatttgg tcaaataaga agattagttt
     3421 actctcggat tttacattta aatcttattt atacttagga aatcaacgag atagagcggg
     3481 agatggcatg acctttacat tgactggcga tcctagaatg gctagtaatc ccagtcaagt
     3541 tattggatcg ccaggtatgg gaattggctc atattctact cgttctggcc aaccttacat
     3601 tgcgaatgcg ctctcgattg agtttgatac gtataaaaat agtggttctt ctgatcgaat
     3661 ggatcgagag ataactgctg ataatagagg gcatggacat gtcgcttttg tcacgcctaa
     3721 accaaataat aataattatt ctggagagca tactggcgtg acggtagctc ctacatttct
     3781 ttctaatggc acatggcgct tgttaataat taaatgggac gcggagcaaa gaagtcttac
     3841 ttatgatttg gaaggtgtcg gtcgctcttc tcggattata cctgatctca ttaggcagtt
     3901 tggttctaca gaagtaaatt gggggtttac ttcttcaaca ggtacttttt ttcaagaaaa
     3961 tgctgtcgct gtcacgcgac ttccatcgaa tgtcacatca acagctgatg taaaagtcaa
     4021 cgatagtgat tatggggcca tcgctgaagc tggaggaaac gataagataa ctatcagaaa
     4081 tacattgaag gtcgaggggg attttcttga atataagaaa atgcagacta gtatcgacct
     4141 cccacaagaa ttaactattc ccgagcatat cttgatcaat ggaaatcaaa ttgatgccaa
     4201 agatatcttg ataaccggaa atcgagtcac gctaaatctg acagattatt ttattgctgg
     4261 agatacgtta ataatcgaat tggagacaac tttgcaggaa gtaattcctg aaaaagtact
     4321 cactatgcat tttgaatacc ttgaaaacgg gaatttgatt gagcaatcga atgaaataaa
     4381 cctaaagatc gcaaagctga aggagataga tatccatgtt tattataaag ataaagacag
     4441 tcagattgat attgctgaaa tgaaaacaat cactgggaaa ataggagaga agtataaaga
     4501 aactcctatt gatattgaag ggtatgtttt tgactcggat tcagcgaata cagaaggcat
     4561 cttctccgaa aatacagagg atatctattt ttattacaga aaaggcaaac tttatctctc
     4621 aactgctcct caatatgtca attttggaaa acataaaata tccagtagac cattaagtga
     4681 attcggacag ttttcaggtg ggttgaaaat tatagatgaa cgggcttttg gaagttggaa
     4741 attacaattg aaacaagaga aagttcttac aaatggagat tttgagcttc cagaagctct
     4801 ttcctttgtt acctctgaaa atacaacggt tatagggaca tccgctgtta cgattttcga
     4861 aagtgatcaa aagggtgaaa gcgatttgtc ctctctttta gattctacag ctcatcgtgg
     4921 aatcagaata aatattcctg tagaaaacca aagaatcgga acctttgagg ggaaactatc
     4981 ttggatactt gcagatgtcc caggaaatta acaggaagag aagaatataa caaaattatt
     5041 gagtttcaag aagggcacag gtaccatagg ctcttgtgtc ccacagagac aagattttgt
     5101 tatagtcttc tcttacatat gattaaagga gcgattttct tgagaccaaa acaacaatta
     5161 tttataacac tcctagttat tttaggggca ctttttaaca atatatcagt gtcagctagt
     5221 gcatttaatt tttcagtgac gccgataact tcagagaatc aaatcgacaa gagaaaaact
     5281 tacttcgatc ttcaacttga tccggatcag gaagtagaag taaaagcgga attgagaaat
     5341 gatacagaaa aagaagtgaa aatcgatatt tctgtgaata gcgcaaccac caattcaaat
     5401 gtgatggttg aatacggaaa aaacgagatt gaaaaagaca tgagcctgat ctttgatctg
     5461 gtagattatg tgagctatcc acagatagtg acgctaaaac ctaaaagtgt ccaaaccgtt
     5521 gcttttcatg tcaggatgcc gaatgagcga tttgatggtg tattggctgg tgggatcaca
     5581 ttccaagaaa tccagacgga aaaggaccaa acagaaacta aggatcaaag cttatcgatt
     5641 gagaatgaat acacctatat tattgctttg ttgatgcaac agacactgaa cgaagttgct
     5701 ccgaacctta ctcttcatga agttaaacca gatcaaatca atgcaagaaa tgtcatctta
     5761 gcaaacgttc aaaatgatca aaaaacctat atcaatcaag tagttattga aacaaaaatt
     5821 acaaaaaaag gacattctga agttttatat caagaggaaa aagaaggatt gcagattgcg
     5881 ccgaatacaa atttttcttt tccaactgct ttaaatggtc aaccgctaac cccaggtgaa
     5941 tatcacctta caatgactgt tttaggaaat gaaaatgaaa atggaaaatt ttcgagaaaa
     6001 aaaggaaata ctacgatcaa ctataccaat caatgggtat ttgaaaaaga ttttacaata
     6061 gacggaaaag tagccaagga attgaacacg acggatgtta cgattaaaaa agacaataat
     6121 tggatttata tcttagtagc ttcacttttc ttattgttta ttttattatg catacttttg
     6181 tttgttttga gaaaaaaaga agagaaagaa aagtaaggtt aagataacaa atagtaaatt
     6241 tttattattg taggatggtt gggctctttt tataacaata taaaatatac cattttatta
     6301 ataaaaatgc atactagtaa atgatataat tctaataaca ggtttgatcg cgagaaaggc
     6361 caaataaaac gctctccaaa atgttaaaaa aaagacagga cgatgtatat tatacaaaca
     6421 agaatcgctg acaacagtga ctcggatcca ggaaatcagt tttatcccca gctagttggt
     6481 gaatgcatgg aaagtaatat acgtagaaag agctgatgac taatgaatta tgagtttttt
     6541 ttaagcaaag aagatcacag aagttattca ttacttaaat atttagaagc aagctcaaat
     6601 ttatcagagt caatatcgaa tgttcaagaa gagttgtcgc tatcgacatt tctcttgaaa
     6661 aaaacgatcg ataagctgaa acatgattta caaaagttcg atctagagaa aaatttgcga
     6721 gtaaccgtca gtgaattgga tatatgtctg gaaattaatg gaaaatgttc tagcaaagct
     6781 ctgttatctc gttatataac ggattcttta tctatgaaaa tggtattggc tttctttcag
     6841 gaaaaatata tctcagtaga aaattttgct aaacagcatc acgtgagtt
//
LOCUS       DACESX010000131         6657 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.131, whole genome shotgun sequence.
ACCESSION   DACESX010000131 DACESX010000000
VERSION     DACESX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.131"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            302..679
                     /locus_tag="IXF88_002320"
     CDS             302..679
                     /locus_tag="IXF88_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1509030.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            667..1005
                     /locus_tag="IXF88_002321"
     CDS             667..1005
                     /locus_tag="IXF88_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1509031.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            1139..1582
                     /locus_tag="IXF88_002322"
     CDS             1139..1582
                     /locus_tag="IXF88_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1509032.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1844..2659
                     /locus_tag="IXF88_002323"
     CDS             1844..2659
                     /locus_tag="IXF88_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1509033.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2669..3331
                     /locus_tag="IXF88_002324"
     CDS             2669..3331
                     /locus_tag="IXF88_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1509034.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            3536..5200
                     /gene="alsS"
                     /locus_tag="IXF88_002325"
     CDS             3536..5200
                     /gene="alsS"
                     /locus_tag="IXF88_002325"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1509035.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            5213..5923
                     /gene="budA"
                     /locus_tag="IXF88_002326"
     CDS             5213..5923
                     /gene="budA"
                     /locus_tag="IXF88_002326"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1509036.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            6053..6550
                     /locus_tag="IXF88_002327"
     CDS             6053..6550
                     /locus_tag="IXF88_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1509037.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         2200 a         1047 c         1389 g         2021 t
ORIGIN      
        1 taaaaacata atatccatat tctctaaagc aattcccgcc gatgtcgata ataataactt
       61 tcgctgatac gttgtcattt ttatacccct tttcctaaaa tttccctaaa ttgttaaaag
      121 tatatccaaa aaagaataaa aaaacaagac ttttttattt gaaataatac acaaacaacc
      181 gatatagata atccagctta gttttaatag aaatgagtat tgacttggag tacacttaaa
      241 gttgtagaat aaataaaata cataagtaaa gtggcaagaa attaagagaa agagtgatca
      301 gatgaatatc aaacaagtca gtgaagaaaa gggcatttcg gccgatacat tgcgctatta
      361 tgaacgaatc gggttgattc caccagttaa tcggacaaaa ggcggaatcc gtgattatac
      421 agaagaagac ctaaaatggg tcgactttac gatctgtatg cgaaatgccg gattatcgat
      481 cgagtctttg attgaatata ttcgcctatc tagcgaagga gaagcaactg tatttgagcg
      541 gcgtaagctt ttgattgaag aaagtgaact tttgacgaaa aaaattgctg aaatgcaaga
      601 atgtttggaa cgtttacaag gaaaaatcaa gaaatatgat cgtgtgttat ctagaaataa
      661 aatggaatgt atcaagtaaa aaaatcaagg agcatcgcta tggagttacg cttactaaaa
      721 tatttttgga caattgcaga agaaggaaca atctctcaag ccgcagaagt tctacatctt
      781 acacaaccaa ccttaagccg acaactgaag gagttagaag aggaactagg aacagaactg
      841 tttgtacgcg aaaaccgcaa aatgatcttg acagaagcag gttattttct aaaaaagccg
      901 agcagaagaa attttagatt taacgaatca aaccactcaa gaattcgaga acagaaaaaa
      961 acaattattc agcggccgat ttacgatcgg ctgtgtggaa gctgataatt cgaatacatt
     1021 ggcactatta ctagaaaagt tgatccgtga ttatcccagc gttcaatttc atatccacag
     1081 cggaacaagt gatgatatca cggagtgact agaaaaaagc ctgttagatt tagcgatttt
     1141 gctagatcca gtagatacag agaaatacga aacgattttg ttaccaagaa aggaaaaatg
     1201 gggattgctc attttagaaa attctgtttt agcaaaaaaa gacgtgataa caaaagaaga
     1261 ctttgcgaat gtacctcttt tgatctcaaa gcgtccagaa atacagcgaa tgtttgctga
     1321 ctggttaggc ataaaaaagg agcagttgct gatcacagga acgtacaatt tgattttcaa
     1381 tgtatttgcg ctaattgaga atcaggtagg tgctgcttta gtcatagaag gtgctgtaag
     1441 tcatctttca aagcgagtga cattcgttcc gcttgaccca atgattgaaa cgaactgcat
     1501 gctagtctgg aaaaagaatc ggattttatc acctgtagtc aatgaattga ttcaccgttt
     1561 caaagaagct tttgaacagt aataagtatc tctaatcgaa gaaatttcaa tacaaaagcg
     1621 agacgtatga cagtcttgct tttgtatttt ttcattttta aatcaaaaaa aataaagaga
     1681 tttacataac ttgttaaata agcgcttaca aacataagaa gaacggaaat gaatcagcta
     1741 taataaccgt tgttctggat tgtaatcatt acaaagaaat gaagattata actttggagg
     1801 tgttcgaaat gaaagaaaaa aagcaaaaat tcgttcaaaa aacatgccct ttattcaatg
     1861 gttgggtatg attctgctga ttgtattagc tttattgacg atcattcctt ttttatggat
     1921 ggtttctgct tcattcaaag caaataatga agtgttcacg attccgattc aatggatccc
     1981 taaaacttgg catttagaga attatctggt tatttggtct cgtattcctt tagtgacatt
     2041 ttttaagaat acagcttttt taagcgttgt gatcacgttg attcagttgt ttacttctag
     2101 ttttgctgct tacgggtttt ctaaactaaa ttttaaaggg cgcgatattt tatttttagc
     2161 atatatcggc acgattgctg tcccttggca gtcttatatg atcccgcagt tcattatgat
     2221 gagaaaagtg ggtttaacat atacgttgtg gtcgttggtg ctattacaag cgttcaatgc
     2281 ttttggtgtg ttcttgttga agcaatacta cagtagtatc ccagattctt tatgtgagag
     2341 tgcgcggatc gatggcttat cagagtgggg aatctatcgc aagatcattt tacctcttac
     2401 aaaaccggcg attgccagtt tgacgatcat cacattcgtt aatacatgga acgattatat
     2461 ggggccattc atttatttgt cttctacgga aaataaaaca atccagttag ggttgaaaat
     2521 gtttattggg ctatttgatg cagagtacgc attaatcatg gctgcctcag ttgtttctgt
     2581 tcttcctgtt gcttttatat ttttagcgat gcaaaaatat tttgtagaag ggatcgcttc
     2641 atcaggaatg aaggaatagg aggtgattat gaaagagcag cgttttgaca aaaatgttta
     2701 tatggaattc ttttattggt tgtatcaatc attggcaagt agcctttgtt tttggtgtgt
     2761 caatatcttg tatatagcag ctgcgttcct tttgtcgata gatggaagga atgtcttttc
     2821 ctttattttt tctttacttt ttattggtcc aggttgcatc gtgatgatag gtatgacaga
     2881 agaattagca aaagaaaaaa gtttttcacc agtacgtttg ttcataaaga cgtttcgcca
     2941 attttggcta aagggatttt tttattggct atttgcttgg atagttagtg tgataatgat
     3001 ttttgattgt ttctttttta tacgtttttc ttatggaaaa tggttgattc cgttgtttgt
     3061 tttacttgct tgtttatcag tttcctttag catcaactac tggtattttc aggtgagaaa
     3121 tccagcgtcg aaaccaaatc aagttctacg gatagcgttc tactatacgt tgaaaaaatg
     3181 gtatgtgagt ttactagatt ttttactatt gacaagtttg tttctcttct ttttcgtaaa
     3241 gccccaatgg tgtattctac taggtccttc tatcgtattt ggtttgattt acttcaacaa
     3301 tcgtaaattg atgagaacta tggatctgta gactttataa attgaaactt tcaattaaga
     3361 atgacagttt ttatgtaaaa actgtcattc tttttcattt tttgaaacat atggctaaac
     3421 aaatgaaata aaacgtgcta tacttttgaa tgaaacaggt ttagaaactg tatcaattat
     3481 ttgtgattat ttctaactaa taattaaaaa taaagcataa aaaggacgat gttcaatgga
     3541 cgaaaaaatg aaacaaggtt cgaaatctgt agtagaaagt ttgattaatc atcatgttga
     3601 ttatgtattc ggtataccag gtgcaaaaat cgatggtgta tttaatgaat tagaggatca
     3661 aggtcctgaa ttaatcgtga ctcgtcacga acaaaatgct gcatttatgg cacaagcaat
     3721 cggacgtatt acaggagagc caggagtagt tatcgcaaca agtggtcctg gtgctagtaa
     3781 cttagctaca ggtttggtaa cagcgacagc agaaggtgac ccagttttag caattgccgg
     3841 acaagtaaaa cgaagcgatc tattgaagtt gacccatcaa agcatggata atgcagcact
     3901 gttccagcca atcacaaaat acagtgcaga aatccaagat ccagaaacaa tttcagaagt
     3961 gattgccaat gcttatcgta tggctaagag ttcaaaaaaa ggcgcgagct ttatcagtat
     4021 cccgcaagat gtagtggatg cacctgtcca agggaatgtc atcaagccat tgagcgatcc
     4081 taaattaggt tctgcttctg cagatgatat ccgttaccta gctgaacgca ttagagaagc
     4141 aaaactaccg gtactattag taggtatgcg aggatcaagt gaaaaagaaa cgttagccat
     4201 caggcaatta gttggaaaga cagcattgcc tgttgtagag acattccaag cagctggagt
     4261 gatctcacgt gaattagaag cgcatttctt tggtcgtgtg ggtttattta gaaaccaacc
     4321 aggagatatg cttttgaaac gaagtgatct agttattgct attggctatg atccaatcga
     4381 atatgaagca agaaactgga atgcggaaaa agatgcgcgg atcattgtga tcgatgaagc
     4441 gccagctgaa atcgatcctt ttatgcagcc tgaaagagaa ttgatcggtg atatctcagc
     4501 cactcttgat ttattgacag gttcattaga gcctcaacaa gtgtcagaag atgcaaaaga
     4561 atatttagca tctcttcaag cagaattaac ggagcgggac attgttcaat caaaaggaga
     4621 agcaggtatc ttgcatcctt tagaagttat taacacgctt cagtcaaaag taacagatga
     4681 tatgactgtt accgtagatg taggtagtca ttatatttgg atggcacgcc actttagaag
     4741 ttatgaacct cgtcatttgt tattcagtaa tggtatgcaa acgcttggtg ttgcattgcc
     4801 atgggcgatt tctgctgcat tggtgcgtcc aaatacacaa atcgtatcag tttccggcga
     4861 tgggggattt ttattctctg cccaggattt agaaacggcg gtacgtaaaa aattgaatat
     4921 cgttcatttg atttggaatg atggacacta caatatggta gagttccaag aaaaaatgaa
     4981 atatcagcgg gcgtcaggtg tagactttgg tccagttgat tttgtgaaat atgcagaagc
     5041 ttttggcgct aaaggaatcc gagctacaag tgtagaggaa ttagaaaaag ctttagaaga
     5101 aggatttgcg acagaaggac ccgtaattat tgatattccg attgattacc gtgataatga
     5161 gaaattggga gaaacaatct taccagatca attctattaa ggaagtgtcg acatgactga
     5221 aaaaatctta taccagcacg ggacattagg agctttgatg gctggcttga tggatggaac
     5281 agaaacgatt gctcatatat tagaaaaagg aactctggga ttgggaacat tgcatgggct
     5341 tgatggtgaa gtcatctttt tagatggcgt agcttatcaa ggaagatcag atggctcagt
     5401 tgcccaatta gatggaagtg aacttacccc ctatgcagca attacagatt ttacgccaga
     5461 tactagtttt tctgtctcaa aaactgcaga tggagaacaa ttgaaaaaag acatattagt
     5521 aagagaagcc ggggaaaacc tatttttggc agtgaagata actggattat tcaaaaatat
     5581 gcacatccgt atcatgccga agcaagaaaa accttacaga agactagcaa aaatttcaga
     5641 gtctcaacca gaattccaac aaagcaatgt ccaaggaaca ttagtcgggt tctttacgcc
     5701 tgaattattc caaggagttg ctgcagcggg atttcacctg cattttatcg atgatacaca
     5761 tacatttggc ggacatgtaa tggattttga agtggctgaa ggaaaagttg agattagccg
     5821 catctcgtcc cttgtacagc attttccagt tgatgatccg acttttgttc acgaaacgat
     5881 tgattatgct gatttagcag cagaaatcca acaagcagaa tagaaaaaga cagcgagatg
     5941 cgttcaaggt gattaacctg acgtatctcg tttttttgag gagaaaacct agcgtttaca
     6001 aaaaaactga gctataatct tctccataga tcaaaaagga gcggttttta tcatgtggac
     6061 agaaaaacag aaaaaagatg catacgaaat tttattatta cagcaacaag gattattaga
     6121 agcagaagat aactggttag caaatttagc aaattcatct gcattgttga atgagacttt
     6181 gcctgagact gtctttgcag gatattattt attcgatggt aatgaattga ttttaggacc
     6241 gttccaaggg cgagtttctt gcacacgtat caagatggga aaaggagttt gtggagaatc
     6301 agctgaaaaa caagaaacat tgatcgttga tgatgtcaaa acccatgcaa attacatttc
     6361 ctgcgattca gccgcacaat cagaaatcgt agtgccaatg gtaaaagaag gtaatttaat
     6421 cggtgtttta gatatcgatt gcggagtaac gaaagggtat gatgaaatcg atcaagcatt
     6481 tctagaaaaa tatgtagatc ttttattagc aaattttaat ataaatgctt tttcatttat
     6541 taataactaa aatcgtctat tgaaacaaaa aagaagagaa atcaagaata aaaagagccg
     6601 gaaagacagt gggcatttct gtttttcggt ttttttattt ttttataaaa tcacaag
//
LOCUS       DACESX010000132         6626 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.132, whole genome shotgun sequence.
ACCESSION   DACESX010000132 DACESX010000000
VERSION     DACESX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.132"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..444
                     /locus_tag="IXF88_002328"
     CDS             <1..444
                     /locus_tag="IXF88_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAQ1509038.1"
                     /translation="NYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKAN
                     IFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDL
                     SMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            725..1945
                     /locus_tag="IXF88_002329"
     CDS             725..1945
                     /locus_tag="IXF88_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1509039.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1942..4662
                     /locus_tag="IXF88_002330"
     CDS             1942..4662
                     /locus_tag="IXF88_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1509040.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4646..5590
                     /locus_tag="IXF88_002331"
     CDS             4646..5590
                     /locus_tag="IXF88_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1509041.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5659..>6626)
                     /locus_tag="IXF88_002332"
     CDS             complement(5659..>6626)
                     /locus_tag="IXF88_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1509042.1"
                     /translation="VLTLAACSGDTNKDIATMKGGTITVSDFYDEAKLESSNQSLVQR
                     MIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAGYTKDTYKEYIRNNLA
                     FEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDVKKSADDGDDFSKLAK
                     DKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDVITTTNPTSYATEYYV
                     VKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEELKDANVKIKDDAFENV
                     LSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDSSK"
BASE COUNT         2336 a         1076 c         1273 g         1941 t
ORIGIN      
        1 aactatgagc ttgagtatga aaaatctatt tgttcaatcc cttataataa tattgaggaa
       61 attatagaaa aggatgctat cagcatttac aaatttttta tttttattcc taatcgtcaa
      121 ttgaaagcta atatatttaa ccatttgaaa aagatgctaa atgtgtctgt tactgaatcg
      181 gcacctgtaa atattgaaat cgtacctcaa aatgtatcta aaggtgtggt gtttgatcgt
      241 ttggaatcaa tctacaattt aaagaaacct ttgagaatcg cgatcggaga tagtttaaat
      301 gacttatcta tgtttgaaag tgcagatatt agttttgcaa tgggaaatag tcatcaagaa
      361 atcatgaaaa aagctgatta tgtcactgtt tcaaatgata aagacggagt atctcaagca
      421 tttgaaagaa ttcttaccat ataaactaat taaggtagta tagaacatag aattctaaat
      481 acggaaaaat ggagcataaa ttccagtaca aatagtactt cttcagtgtc aaacgaagcg
      541 aatataacat agatatttag gattgtgaca aaaatcatgt cacaatcttt ttttcgaata
      601 aacttatttt ttgaagtcgg ccatttatcg taatttcttt gcataatacg aacaaatttt
      661 cggttattga tgaaaaaaaa cattgaaaat ggtatgataa aagaaaaaaa ggaggaaatt
      721 tttaatggtg aaatttatcc atgcggcaga tttacatatg gatcggtcat ttgaaggact
      781 gacaactctt gataaaactg tacaggaaaa actattgaag acgaatctga ccgttttatc
      841 aaatatcatt gaacaggcaa taataaataa cgttgatttt gttttacttg caggagataa
      901 ttttcatcaa aatcgtcctt cattaaaaat acagaaacat ttttcagaac agatgaaacg
      961 gttagaaaag aaccatatac ctgtatttat aatattcgga aatcatgatt tctatcaaaa
     1021 agagcggtat tggttctcct ttcctaaaaa tgtccattta tttacatccg aaacggtaga
     1081 gacaaaaaaa ataacgataa aaagtggaga aactgtctct ctcagtggat ttagttatcg
     1141 tcaaccgtgg attcaaaaag acaaagtgat ggaatttcct tctagagaac tcacagatta
     1201 ccatattggg ttgtaccacg gggaacctgg cgtctctcaa aaaggaaact atgctccttt
     1261 ccaaccctca aagatgcaag aaaagggata cgattattgg gctttagggc atatccatgt
     1321 gccaacggtc ttaaatgaaa agcaaacaat tgtttatcca ggtgcgcctc aaggtcatac
     1381 taaaaaggaa caagcttcta catctattct actagttgaa ttaagtaaag gaagttgcca
     1441 aatacatccg attaaagtag cagaggtcta ttggaagaca aaggaaatat cattgaggaa
     1501 agcaagaacg acaaaagagg tcattgttca cattcgtcag cagctttcaa aagtagagga
     1561 tggatttagt ttaattgaga taaaattaaa agattatgac catctcaata cagatgtttt
     1621 ggaacggata caatcaggag agctattaga ctatttgtta gaggaattct cagataggat
     1681 aaatgatttc tttatttggc gtatttctct catagagaat actttttata cgaagacccc
     1741 acttgctgct tctgctaaat tgatggaaca gttatttcaa tactatcaga cacctcaaga
     1801 tttccaacaa attttaaatg aagtatatag tcatcaagaa gcggctagaa tattgagcga
     1861 actgccggaa tatcaagaag aaacacttga aaaagccaag cagctgctta atcaagattt
     1921 tttatttgag gaggatcagg aatgaagatt ttaaggatcg agattgctgc ttttggaaaa
     1981 tggcgtcaaa aaagttttga cttttattca ggcaatcagc tgatctatgg gggaaacgaa
     2041 gcaggaaaat caacgattta tcaatttatt caagcaatat tatttggttt cccttcaaaa
     2101 ggccgtaaaa aaaaggatta tacaccaaaa gacggctcag cctatggcgg caaaatatgg
     2161 cttaaacatc ctgtatatgg tgaatttgct gtcgagcgct acaagcagca aaatcgcgga
     2221 aaatcgaagg tgtggctggg ggatcaagtg ggaagtgatg agttactaga aaaactgatc
     2281 gcacctttga cgaaagagtt attccagcag gtatttactt tccagcaaga gcagttgatg
     2341 gaactagatc atttgagaga aaaagagcta catgatgcgc tgatttcttt aggcatcacg
     2401 ggcagttctc aagtattcca aaagcggcaa gaatattatc agaaagcaca acaattattc
     2461 aagaaaaaag gccaaaaatt actaatcaat caaaaattaa atgaatggaa agagctgcaa
     2521 gaaaaaattc atcaaaaaca agaacaggaa gcttattttg gcaagttgat ccaacaagaa
     2581 caggaatacg agtcgcaaca gcagcagtta ttggcacaat taaaagaggc gaacaaacaa
     2641 ttttcattag ccagacagca agaacttaat ttttctttat atgaagaatg gcaatctctc
     2701 aaaaaaaaca gcactacagg tcttcctgat gagcaacttc ttttagatct ggagtctttc
     2761 tccaaacggt atcagcaaac aaatgagcgt ttggaagaat tagaagcaac actggaaaag
     2821 catagcggga tggatcagca gtcagccaga tattattttt atttagagca ggagcaggaa
     2881 ctaaaagatc tccagcaact tcaatttaca gcggaacaat taaacgaaga atggcaaaga
     2941 ttacaaattg agactagaga aacaaattca gaattgaaga ttttggaaca aaggtggcat
     3001 tggcagaaaa atccgttacc ggaagaattt ttatatgaag aagaatggaa gcatcttctg
     3061 gctgaagaaa gaaggattgc tgatctagaa agccagattt ctgtgcgtct ccaaatagct
     3121 aaagaacagc aagagatgat tgaagcagaa gtgaatgact tggaagagac ctatccagca
     3181 ttattacaaa atcagtcaga gatagcacca gtctcttcat ctaaaccgta tatgctgatg
     3241 gctggcggag caataatcat tgcaggtata tttgtaccaa tgcccttgaa agtgatcctt
     3301 ttactaattg gtgcaggcat gattggctat ttcttctaca aaaggcaacc aaaaattaaa
     3361 actaaaaatg atttctctga gataaaagga atgtggcaag aaaaactagg acaacttgat
     3421 ctagccttag ctcaattagc tgaagcagaa gaaaaacaaa agagaactgg agaagaaaaa
     3481 cagattcttc agcaagaagt atatcgtttt gcggatcatc ggcacttagg aaagatggat
     3541 acgctcaaac tcatgaaaga atatggacag gaagtcatag attatcagaa attatatggc
     3601 gtatttgagc ggaaacataa aagagaacag gaaatacaag aaaaactacg acaaatcgac
     3661 caaaaatttg agtttgttaa agaatggtta ccgatacaag ataagacaat ttcagaaaaa
     3721 atggtattgc tttcccgttt tatacaagag atggaacaga tgaaatttgc cagaagctat
     3781 cagcaaaata ctttattgaa gcaagaaatc aatcaattga aaaagaaaca aaaagtatgg
     3841 atacaagaat acagcgaaca attgactcaa gcaggcttat cttatccttc ggaagtacct
     3901 gtctttttac aaaaagccaa acaagccgaa cagcagatga aaagatacaa tgaattatcg
     3961 gaaatgctta ctccactatt tcctgaagag ctgacaaaag agaaattatc tgtaagactg
     4021 cgccaactga aagaaaagca atcccaactc cagcaagaag aaaaagaagc aagtgaaaat
     4081 cggcaacgac tccaacttca agtagaagag ttgcaaagag acggcacatt ggacgaactt
     4141 tatcaacaag ctagccggca aaaagcaaaa ttacaggaat tactggagga atggggagca
     4201 cttgaaatcg ccgggacatt cttaggtgat ctttcaacag agatgagtga aagacaatta
     4261 ccccagttgt taagagcagc aggtgattat ttaggtattt taacaaaagg aaattatcag
     4321 aagatattac tgaaaaatga tacactgact ttaacagacg gatatacaga atttcccatc
     4381 tatgagttat ctacaggtac gaaagatcag ctgattatgg cgatgagatt tgcttacctc
     4441 tcattagaag ctaaccgttc gatatgcccg atcatcattg atgatggctg gcttcattat
     4501 gatcatcagc gaaagtatca attagctgaa cttttagcta aatttggaga aaagtatcaa
     4561 ataatttgtt tttcttctga taaagaaatg gtaagctatt accatgagat gaaccaaatc
     4621 gtaaaacaat tagaaggagc aaataatgaa aaaaattcgt gatttagtaa tcgatgagca
     4681 gtttgaacaa tttgtcctga tcaaaagtgc agatgtaaga atcgctaaaa acggaaaaaa
     4741 atttattgct tttactttcc aagatacttc aggtactatc gatggtaaat actgggatgc
     4801 ttcggaggag gaaatcaata aatttacagc cggcacagtc gtttttctaa acggtaaaag
     4861 agaggtttat caaggaaacc ctcaagtaaa gattcttcat atgcgcttaa caagggtaga
     4921 tgagccagga gagcctagtt tatacatgga aagagcccct ttgaagaaag aagaaatgga
     4981 agaagaaatc aatcaattgt tgtttgagat caccaatgcg cattggaatc gtattgttcg
     5041 ctatctactt aataaatatc aaaaacaatt ttttgagttt ccggcagcaa aaagaaacca
     5101 tcatgcattt gccagtggat tagcttatca tactttgaca atgcttcact tagggaaatc
     5161 aatcgccgat gaatatcctg agctgaatcg agcattgttg tatgctggta tcattttgca
     5221 tgatttagga aaggtgattg aactttccgg tcctatgtcg acagaatata cgcttgcagg
     5281 aaatttgatc ggtcatctgg tattgattga cgaagaaata actaaagcct gtcttgcgtt
     5341 gaaaatcagc gaagctgacg aagatgttct cgtattacgt catatggttt tagcacacca
     5401 tggcttatta gaatatggct cacctgtacg tccaagaata atggaagcag aaatattaca
     5461 tcaaatcgac aatatcgatg cttcgatgca gatgatgttg acttcgattc gtcaaacaga
     5521 accaggaaaa tacaccgatc ggattttcgg tatggacaac cgaagtttct atgtaccaaa
     5581 agatttatag caaaaattta tttttttcat tcaaaacaaa aaaagcgaga cataagtctt
     5641 cgcttttttt gttttctttt attttgatga atcagttgtt tcttcagttg aagattcttt
     5701 tgttgaatca gttgattttg tgtcagaact ctttgttgaa gctgtagttt cacttgagtc
     5761 tttcgtactt gatgaagttg ttgtgaatgc actcaacaca ttttcaaagg catcatcttt
     5821 gatttttaca tttgcatctt ttaattcttc accgattact ttagttgtga atgcattgtc
     5881 acttagttta gtttcagtag cgatatcttt tagttgatct ttgtatttat ccatgtcatt
     5941 gcctttattt tggtttttca ccattttgac aacgtagtat tctgtcgcat aagaagtagg
     6001 atttgttgta gtaataacat cggagatctc accgtctttc aatttgaaag ctgcttcttt
     6061 tacttctgca ggaatagttg ttgtcgttga gtcgaattta actttaccgc catcttcttt
     6121 tgtctctgta tcagttgatt tgtctttggc taatttagag aaatcatctc catcatccgc
     6181 tgattttttc acatcttttg cttcatcttc acttgataat ttgatgattt gtgcttctac
     6241 ttctggatgg aaagatttcc aagcagtttt caaatcgtca tctgtaatat ctacgtgagc
     6301 tttcaagcca gcttcaaaag ctaaattgtt acgaatatat tctttataag tatctttcgt
     6361 atatccagca gcttctaatt gtgattcaaa agtatcacct aatgatttcg cttgtttgtc
     6421 gtattctgca tccacttgct tatcggttac tttatcgcca tatttgttat tgaatacttt
     6481 ataaatgatc atccgttgta ctaaggattg gttcgatgat tcaagttttg cttcatcata
     6541 gaaatcagat acagtgatcg ttccgccttt catcgttgcg atatctttat tagtatcgcc
     6601 tgaacaagct gctagagtta aaacag
//
LOCUS       DACESX010000133         6614 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.133, whole genome shotgun sequence.
ACCESSION   DACESX010000133 DACESX010000000
VERSION     DACESX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.133"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..336
                     /locus_tag="IXF88_002333"
     CDS             <1..336
                     /locus_tag="IXF88_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509043.1"
                     /translation="SVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKS
                     ISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNN
                     NFYFYRNKL"
     gene            complement(462..1139)
                     /gene="nth"
                     /locus_tag="IXF88_002334"
     CDS             complement(462..1139)
                     /gene="nth"
                     /locus_tag="IXF88_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1509044.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1146..1844)
                     /locus_tag="IXF88_002335"
     CDS             complement(1146..1844)
                     /locus_tag="IXF88_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1509045.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2112..3296
                     /locus_tag="IXF88_002336"
     CDS             2112..3296
                     /locus_tag="IXF88_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1509046.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3302..3745)
                     /locus_tag="IXF88_002337"
     CDS             complement(3302..3745)
                     /locus_tag="IXF88_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1509047.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3971..4258)
                     /locus_tag="IXF88_002338"
     CDS             complement(3971..4258)
                     /locus_tag="IXF88_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509048.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4429..5781)
                     /locus_tag="IXF88_002339"
     CDS             complement(4429..5781)
                     /locus_tag="IXF88_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1509049.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            complement(6053..6499)
                     /locus_tag="IXF88_002340"
     CDS             complement(6053..6499)
                     /locus_tag="IXF88_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1509050.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1797 a         1307 c         1090 g         2420 t
ORIGIN      
        1 tctgtacttt taagcactac tcttaaagga aaatttatgg aaagaagctc tcatgcaatg
       61 attctcactt ttttatggtt gttgttcacc agaatctata tttttctgat gagcctgaaa
      121 ccgcccaaat ccattagttt atgtttagtt ataaatggta tttttttatt atcacttatc
      181 atttttatta cacctttaga tagatacgtc acttatttat ataatccttt ggaatactgg
      241 acttatttta taggtatatt ggaaggcatt attttctaca tcgggtattt tcccaataaa
      301 aataacaact tttattttta tcgcaataaa ttgtaataag ttaccatcct agctactgag
      361 tatgtacatt tactttttca tgcaaaaaag ataaacgaaa cagcttttct ccataataac
      421 aagataattc atacatttat ttgcaattaa agaaaccacg tctacaggcg tggtttcttt
      481 tttagcgcct tttcttttag acgcatccga tttttcccat cttgacatat tgatagcaaa
      541 ggacatacct cgcacttggg atttcttgcc gtgcaatgat atctaccaaa aaaaatcagt
      601 gtatgatgtg tctttaccca taattcttgt ggtactttcc gcatcagtgt ttcttctact
      661 tccatgaccg tagcatctag tttgcagatc ctcaggcgtt ttgagacacg ttccacgtgg
      721 gtatctactg cgattgctgg tatcccgaat gcatccccca atacgacatt tgctgtcttt
      781 ctgcctacac caggaagaga catcaattct tctcttgacg ttggcacttg cccatcgaaa
      841 cgttcaatca gctgctgtgc acacgctttg atgttttttg ctttatttcg atacaaacca
      901 atcgttttta ttttaagaat gatttcatcg attgaagctt ctgctaacgc atcgggtgtc
      961 ggaaaagagg caaacaaatc tggcgttgct ttattcactg atacatctgt cgcctgggca
     1021 cttaagatca ctgcaatcaa taactcaaat ggattattgt gcttgagttc tccatgtgct
     1081 tcgggaaaca ttccatacat cgtttctaga gcttccattg ttttttgttt gcttaacata
     1141 ttccgctact cctccaacca atttttcagt gagactttcg gcaattcttc ttgttgagct
     1201 gactgttcta cttctctttg aagaagctgc tgttttcttc gtttttggtc ttcttccact
     1261 tgttgttttg tacgtagatt ttttctttcc cacgatagca aaatgcggtc aacgtaatta
     1321 aaactataag cctgatttaa tacggcttcc cgcagtgcta gcttcaaaat ttccggctga
     1381 taatggtctt cttctagcca ttgtccgatc cgctggaact cgatcgaaga aagcgggcgt
     1441 ccaaattctt gttcaaataa ttgatacact gactggattt ctttttcttg tgttttttct
     1501 tcgtattttt tttcttgtgt ttttaaatac tcacctaata gatcatagat ggggtaaaga
     1561 ttatatcgat ctgctttttt accgttgaca atcgtcgttt ctatcttgat aaatccattt
     1621 gttacgagac gatccagtat ttgaaaaatc tgatcttgtt tgattcccat atccatagca
     1681 atcatctgca aatcagggaa agtatctcct tcaagctgtg ccatatgcaa ctgtagaaca
     1741 aataaaaatt cttcagattg tagaccgatc ctatgataat ttttaagtaa aagattagat
     1801 atgactgttt gtccagcgct taagtaattt tctaaattca acatgatttc tcctccgctc
     1861 aatcacagta tacgtaaaaa aaacatggga agcaagaaca ggtttctttt ctatttactt
     1921 cactgatcgg tcagatttac aatgtcgtct acttgatttt tgtttgttca tgtttttgat
     1981 gcgtatcttc acttttcgga ataaataaat taagtagatt tcgctgttcc ttagaatgtt
     2041 gacaaggaaa caaaattact atacactatt tttgtttcca agttaacatt attgcataaa
     2101 ggagttttta catgtacagt ttcacaaata aacattctaa agtttttgga tacgtctctg
     2161 tcttattcac tggattagga tttactatcg tcagtcctgt acttccgttt ttaacgctgc
     2221 cttattctca ctccatacat cagcaagctt tctatatcac tttgctcatg tctgtttatg
     2281 ctctagctgc cttcttatct gcccccattc tcggtagctt aagtgatcat tttgggcgtc
     2341 atccgatcct tattatcagt ttattaggat caagtatcgg atatttgctt tttggtttag
     2401 gaaattcgat atggatgttg ttccttggtc gtatcattga aggattgacg gcaggagaaa
     2461 tctccacctt atatgcttac tttgccgaca tcacggaacc taatgaaaga accaaagttt
     2521 ttggatggat gggtgcttta gtcggtatag gaacaacgct gggtcctatt atagggggtc
     2581 tattagctga gttggggaat tctgttccta tatttattgg agcactcttt actttcttaa
     2641 atgccgttta tggctacaca tttatgctag aaagccttcc aataaaaaaa cgttcagtag
     2701 atttaagttt ctcccatgta agaccttttc accaattaca acaattattc aagatttcat
     2761 ccgttattcc tttattgaca gcaggttttg ctgtttggtt ggcagctggc tctcttcaat
     2821 ctatcttttc tcaattttcg attgacgcct ttcagtggaa ggctgggttg gttggtttaa
     2881 gcttctcttt gattggaatc ttggatactg tctcgcaatt gttcattatg cctcgattgc
     2941 tcaaaaaatt ttcagaacag cagatcacaa gaatagggat gttcagtgaa atcctcgcct
     3001 atctattcat cacattatct ggaattcttc tactgccgat tttgttttta tttgggatta
     3061 tctgttatgg tttcggtgat tctattttta ctcctgtatt caatggccaa ctatccaatt
     3121 ctgtctcaga taaccagcaa ggtcttgtaa tgggcggtac ccaaagtata caatctctat
     3181 cgcgcgtcat cggcccgcta atcgcaggac agctttatgc tgcagcacca tttcttccga
     3241 cggtattcgg atttttgctt atgatttttg cctattatca ctacaataaa aaatagtagc
     3301 ttcatttgct acttcgtata aattctctgt atcgaccgat aaaacgaaat actgtgtcaa
     3361 attcttcttt tgacatattt tcaaatacag tagcatctcg ctgtacccat ttttgatgga
     3421 gttgctcgtg tctattcctt attttttgtc cgattggcgt caattgaaaa tagatttctt
     3481 ttttattctc ttcgctttga taagtgttta tgatccctct agctatcagt ttttttgtta
     3541 gcttactgat ggctcctcta gtcatgtaac tagcattcgc taattttgtc acgttaggat
     3601 cagggatttt cccgatatgt tcaatcactt ctagttcatt tagagtaaat cctgcaaatt
     3661 ctttttccat ttctggttta ttacgatgaa taatctcatt agctaaatcc agatattctt
     3721 ctattagcag ttgtctttta tccatttcta caccttcttt ttctttcatt atattgtttt
     3781 tttgtttcct attcaacaaa gataaaaata ttttgtagga aaacccaatc tgaaaccaag
     3841 aaaaaaaggg gttgacaaag cttgtcataa cccctttttc tgattaaaag atagtaaaga
     3901 gacagtcatc ggtaattgtg atgatttttt gtcatcactt aaggatgagc ggccttttca
     3961 aaaactgtct ttaaagatca tcatcagttt ctagcacata tggctgacct tcatatgtgt
     4021 actctgtatt caatgtgccg tcattttcat ctgagtagat gcgtctactg aaaggaaaac
     4081 gatgagaaac attatctttg gcgtcatttg ctggcatcgt gtaatattcc tcttgcgttt
     4141 cattcaactg agagagtact tgatctgcca actcatcaat attgatcgtt aatttcttcg
     4201 aagcactttt ataatcttct aaaaatgcat cttgttggac aaaatattct ttattcatct
     4261 tctttctcac ctttcttttc aaaatcaaat aaagctgctt gttcttttat gtaacagctt
     4321 tatctttgct gcttattgtg acaaaaaatt cgaagaaaga caactaattg tacttttcac
     4381 aaaaaaagac agagggtcgt gacaaaattg tgtcacaacc ctttttcttt attgtgcagt
     4441 ttcgatttcg cctagcgctt cttgtgcaag taggttcaag taattccatg ggcggtcaaa
     4501 atgtggttgg aagaagaaat ctgaaagtgc gagatcttct acagtcattt tattttgaac
     4561 agctaaagac atggtatttg ctgattgtgt aatgtcgtat ttggacatga actgagcacc
     4621 tacgatacga ttcgttcctt tttcatagac tagctccatc attacttttt cagtagttgg
     4681 catgaactct ggacgataat tatcttcaaa tacagtcgct tggacttcta gatcgttcaa
     4741 agttgcgctt tctttggtca cacctgtcga accgatcgtc cagccaaata ggtaaagtcc
     4801 tgaagttcct tgagtaccac gatacgccat tttatgatct gtcaagttat ttcccacaag
     4861 taaaccttga cgaacggcat ttgttgccaa aggaatatag ttttgcgtgc cacttggatt
     4921 gtagttgact actgcagagt ctccagctgc aaaaatgtct ggatcacttg tctgcatgta
     4981 ttcattcact ttgattgccc cgtttggcag tgtatctact tttccttcga tcagttttgt
     5041 atttggacgg aagcccacac ataaaatgac catatctgta tcaaatgtat ctgttggtgt
     5101 agctacttgt ttcacttttc cttcttcatc tgtgataaat tcagtaacat tttcgcctag
     5161 cgcaagagta acaccgcggt ctgtcaattc tttttctaag atatctgtga atggtttatc
     5221 tagatattta ttcaagatgc gatctaatcc atcaatcaat gtcacttgtt ttccagattc
     5281 gacaaaagct tctactaatt caataccgat atatccacca ccaacaatta ctacacgttt
     5341 tgcttctttc gcttgtgcaa taatttcatt tgcttgattg tagtttttgc ataggagtac
     5401 atttttcgca tcgattccac taatcggtgg gataataggc catgaaccag ttgtcatcac
     5461 taatttatca taagaaaccg tttctgtttc tcctgttttc agatcttttg ccgttactgt
     5521 tttattttct gtatcgacgt ctgttacatc gtgttccatt ttaactgttg ctcctaatga
     5581 agataattct tcaggatttg agtagaacaa gcctgctggg tcctttacta ctccgccgac
     5641 ataaagtgcg ataccgcaag ataagaaaga aatattgtca tttctttcat atactgtcac
     5701 ttctgcttct ggatgatttt ttaaaatact ttttacagct gcagtacctg catgagtaca
     5761 gccaattact actactttca tttatggtcc ctccgattaa cattttgtcc ttcgacaaaa
     5821 ctactataac agaaataaaa cggatacaac agtttaatgc ttgtgaattc acaaaggttt
     5881 gtttgtagtt tcattccttt tcaagcataa gaacccaata tctacaagat tcttattgtg
     5941 ataatattca ctttgatatc taagattgtg aataaaagac aaaaaaataa aaaagctagc
     6001 gtttttttac acagctagct ttttttaggt ataacactta tagaaactgt ttttacattt
     6061 gcgccttttt tgctgactct ttacaatccg gacagagtcc atacaattcc aaccgatgtt
     6121 cattaatctc aaaatcagtc agtctgctcg cagccatttc tatatcttca agccctgggt
     6181 aatgaaaatc aacaatctta ccgcagtttt cacaaattac atgataatga cgctttgaac
     6241 tgaaatcaaa tcggctagaa gcatcgccgt acttcatttc ttgaacaaat ccaatcgaag
     6301 taaataagcg aaggttattg taaacagtcg caacactcat atttggaaac cgatgttcaa
     6361 gtgctttata aatctcatca gccgttggat gggaatgatg ttcaatcaaa tattctaaaa
     6421 ccgcgtaccg ttgaggtgtg attcgaatgt tctctttttt taacttttcg ataacctctt
     6481 cgactaactt attttccatg taaacacccc acgttgttat tctcgtctta tcatactatt
     6541 tttacatttt aaaatccact taacatgaaa agaaaattca attttattgt attttaatga
     6601 aaaaagacgt aaaa
//
LOCUS       DACESX010000134         6468 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.135, whole genome shotgun sequence.
ACCESSION   DACESX010000134 DACESX010000000
VERSION     DACESX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.135"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            74..955
                     /locus_tag="IXF88_002341"
     CDS             74..955
                     /locus_tag="IXF88_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1509051.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            968..2578
                     /locus_tag="IXF88_002342"
     CDS             968..2578
                     /locus_tag="IXF88_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ1509052.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2655..3314)
                     /locus_tag="IXF88_002343"
     CDS             complement(2655..3314)
                     /locus_tag="IXF88_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1509053.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3597..4262)
                     /locus_tag="IXF88_002344"
     CDS             complement(3597..4262)
                     /locus_tag="IXF88_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509054.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4409..5014)
                     /gene="dhaL"
                     /locus_tag="IXF88_002345"
     CDS             complement(4409..5014)
                     /gene="dhaL"
                     /locus_tag="IXF88_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1509055.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5042..6025)
                     /gene="dhaK"
                     /locus_tag="IXF88_002346"
     CDS             complement(5042..6025)
                     /gene="dhaK"
                     /locus_tag="IXF88_002346"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1509056.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(6027..6422)
                     /gene="dhaM"
                     /locus_tag="IXF88_002347"
     CDS             complement(6027..6422)
                     /gene="dhaM"
                     /locus_tag="IXF88_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1509057.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
BASE COUNT         1683 a         1384 c         1221 g         2180 t
ORIGIN      
        1 aggtataaaa cctttttatt aaactattta atcttgttta gtatatattt ttagaaaaag
       61 gagtggggtt tttatggcag aaatcgtcaa agtcacacat ttgaaaaaga gctttggtcg
      121 tttcgaagct ttaaaagatg tctctttcac cgtacattca ggtgaagtcg ttggatttat
      181 cggaccaaat ggttctggaa aatccacgac gattcggaca cttttaggga ttataaaaaa
      241 agacggcgga acggcaacga tttttgggca agatgtatgg gaagatagtt tatctatcca
      301 tgaacgtctt tcttatgttc caggcgatat ctctttgtgg ggaaatttga ctggaggagc
      361 gatcatcgat ctcttcatga agcttcatgg aaaagggagt gctcaaaaaa aagatgaact
      421 gattcaacgt ttctctcttg atcctaagaa aaaagcaaaa aactattcaa aaggaaatcg
      481 ccaaaaagtg ggcttgattg ctgctttggc tgttgattcg gatctctatt tatttgatga
      541 gcctacttct ggtttagatc ctttgatgga aacgattttc caagaagaag ttgaaaaatt
      601 gaaagaacag ggcaaatcca tcctactttc ttctcatatt ctcagtgaag tcgagcgact
      661 agcggataaa gtcgtgatca tacgcgaagg aacactcgat gaagaaggaa cactcgatga
      721 attgcgtcat ttgactcgtt cgaccatcac tatagaaaca gaagcagaga atagtcactt
      781 agcacaattg aacggtgtcc atgactttgt gcaaaaaggt cacctcgctc aatttgctgc
      841 tgataacgaa gctttcgatg tgattttagc tgaagcgtcc aaattaggtg tgaaaaaatt
      901 tgaatctatt ccaccgactc ttgaggattt attcatgaga cattatgaag gatgaggagg
      961 aagcgaaatg actgataaat tttcacgctg gtccaccctt ttttctcaaa accttagccg
     1021 tgactggaaa aagatcctga tatggctagt cggtgtcact gctttttctg gtggttttgt
     1081 ccctgcgttt gaagaattga cttccggaca aggattagct ggattatacg aaaccttgca
     1141 aaacccggca atgattgcaa tggtcggacc aactccagct tcgaatgctg ctgagtatac
     1201 tttaggagcg atgtatgcaa atgaaatgtt gcttttttgc agtttgtttg ctgctgtgat
     1261 ctctgtttta cacgtggtga gccaaacgag aaaaaaagaa gaacttggtc tgattgaact
     1321 tattcgttcc tttcgagtcg gacgtcaagc aaactcttta gctgtcatcg ctgaaagtat
     1381 tctgatcaat ttgcttttag caatatcgat cagttgtacg atgatttttt tccaagcgga
     1441 ttcgatcact gcagaaggtg cttggctttt tgggctatct gtaggtagta tggggatttt
     1501 ggggagtgcc gttgcactgt ttgctgcaca actgatgcca agttcagcca gttcgaatgg
     1561 tctgtcttta gcgtttttag gtggacttta ccttctacga gcaataacag atgtagctga
     1621 ttcctccctt tcgatgtgga atcctttagg ctggtcttat ttgacctatc catttacaac
     1681 taacaactgg attcctttgc tttatttgtt tttgttcagt ctaatcttga taatcagttc
     1741 atttgtttta gaggaacatc gagatttgaa caccggctat atccccgaac gtgaaggaaa
     1801 accgtatgct aaacgttctc ttttatcctt gcgtggttta ttattctatc taaataaagg
     1861 gatgacaatc agttggctta tcacttttgg tttgatgggg gcggcatacg gctctatcta
     1921 cgggaatatg caacagtttt tagaaagtaa cgacttaatg aaacagatgt ttatccaatc
     1981 tggcgtctca attgaaaaaa actttacaag cacgatcatg gtcgtcttga tttgtctagc
     2041 tgctatttta ccaattgcta gcatcaatcg tttatttaca gaagaaaccc acggacgtat
     2101 gaatcagctg ttcgcaacaa aaatttctag gaaccaactt tattggacca atatgttact
     2161 agctattcta tttgctatat tggcaatcct gattagttct ggtgcactag ggctttctgc
     2221 tttggcttcg atgaaggatg cgaaagacat ggatctattg atgtttctta aagccgggtt
     2281 caatttcctg cctgccgttt tagtgtttat cggaattagt ggctttctat tcggatggct
     2341 tcctcgaatc ggtaaacttg cttatatcta cttagcttac tcctttgcac tcaattactt
     2401 tggcggaatt ttggatttac cagaatggtt ttccaaaaca gctgtcccta gctgggttcc
     2461 acggatgcca aacgattctt ttgatctttc cgtgttcctt acgttatcat gtatcagcct
     2521 tgcactgttc gtccttggtt ctattggtta taggcgaaga gacttactag aaggataata
     2581 aaaaagcatg tacaatttca tgctagcctc caaatctggg tcaaaaatcc aacatttgga
     2641 ggtcattttt ttatttacaa gtcttctaac tgctctttat caatgatcca gacttctcga
     2701 ttctttcttt ttacataacc attctcctct aataatttaa attttctaga taaagtttcg
     2761 ggggtcgtac cgataaatgc agcaagttct ttcattttca ttggcaggaa aaagtgatcc
     2821 ttccctgtcg ctttagctaa gtcaagcaga tagacagcga ctcgttcttc tactttttcc
     2881 atcatcaaaa aagttgtctg ctgctgtact tttgttaatt tctcaacgtt gattgctaaa
     2941 agtttcagac taagggcggg ataatgcagt aagagttccg agaaatcttt ttgtctcaat
     3001 atgcaaattt ccgttttttc tagtgcttcg ccaaataaag actcatttgg aacgcctagc
     3061 aactggctct ctccctcata tccgcccggc tctattactc gtaaaagctg ttcctttccg
     3121 ctagcagata attgatacac tttcatactg ccggaggcca cgatcactaa ttgaggttct
     3181 ccattcggcg caagaatctg ttctcctttt tccacatagt gatgtttgac aagtgcgtgt
     3241 actttttctt gatcttgctg tactaagtga ttgaacaaag gaacaaatga aacacataaa
     3301 tgatcatgac tcataatctc tcctccttct aaacagctat ttctaataaa gaaacataac
     3361 ttcccttatt ccaactgata attttctttt atcatagcat aaacgtcaag cgaaccagat
     3421 accagcaaat agagtggatt tctttttgtc ccttgcttta ttttccttct tttgagacct
     3481 taagtaattg attcctatca attttttaaa tatttacttt ttgaataatc aaaaacaaac
     3541 cttcactcgc aagccgagag taaggtttgt ttttcgcatt gcttatttcg tgttgtctat
     3601 tgaaattttt ccgcacctag cttttgatat agtggtgcta gttcttgtaa ggtttcagcg
     3661 gttatttgct caacgtgttc gatttcttgc ggatagaaga atagtttacc catttgaaag
     3721 gttcggtctc tcatcgcttt tttcaactct tcttgtttct ttgtatcgga caaatagtca
     3781 gacaaagcta agtggtcgtc aagttcatgt tctggttgcg gccatatgta ataatccttg
     3841 atatcgactg ttttgctcca ctctttcagt atcgcaagca actggttata cgctgcaatg
     3901 ctaccatatc gttcttcgct tttataatgt tggtacatca aatagatttg gtattgtttt
     3961 tgattcaaca aaactgctag acaggcattc tcgtcttgac gagattcgct tcggtacgcg
     4021 ctccaaaaat gactgcgtaa attccagcca ttcgtccagc tttcgatttt aggtttgctc
     4081 atgccgtaca tatcaggcaa aagtgcagct gtctggagct gaatctcttt ccatttttgc
     4141 cagtgttctt tatattcttt cttgatttgt tcaatcttat ctgttggatg gctctctttg
     4201 agctgtttga atgcgtagat tggtcgatca aacaactgat aatctgattt tccaagttcc
     4261 attgtttcgc ctctttccgt tatcttttgt attctacctg aattttgcga tatggacaaa
     4321 tagaggttgt gaaaaagctg ttctgcatca agtcataaga acagccaaac aaaaaactgc
     4381 gatgagcatc accgcagttt tattgaactt aaggttgatt gatcacatct aaaagcgttg
     4441 aaaataaaat ggcactagaa gcagctcctg ggtcgatatg tccgattgca cgctctccta
     4501 gataggaagc tcgtcctttt ttagctacca agactttggt gtgttctttt gcctggtcaa
     4561 tgatttcttt tgttaaattc cctgccttca atgcctctgt gactggcagc caaacatcta
     4621 acatcgtttt atcgtcagct tctgcctttc ctcggctttg aatacccgct gttccctgct
     4681 cgatcacttc gcctaatttt tcttgagaat caatagaatc gacctctttt gtcgctttca
     4741 tcatgttcat gaatgcagat ccgtataacg gaccagaagc cccaccgact ttcgatatca
     4801 tcgccataga gaatactttg aatgtatcgc tgattgtttc aggatgatcg gcttgaaaag
     4861 cttcttcata agctgaaacc cctcttccca tgttatttcc atggtctcca tcgccaatcg
     4921 gggtatctag atcactgaga tattgtttgt tctgattgat tgcctctgca aattgcgtga
     4981 gccattcttg gatttcttga acggttagtt tcatattatc acccttttat cttgagattt
     5041 tttaccatga aatcgtctgt acattgcttt ctaaagaatc tttccattgt gtctccgtca
     5101 agtcgatcag tgttagagaa agaccttgca tatctagtga ggtcataaaa ttaccgactt
     5161 ttcggaaaac tacattgacg cctctttctt ccaacagagc taaaacatca ttcatgaaca
     5221 cgaactgttc catcaaaggc gtcccgccca ttccgttgac tagtacacct gcttcttttg
     5281 gtaaattcga gtaatcatct aaaatcttag taacaagttc ctttgccagt actttagaag
     5341 gctgcatctt ttctcgacga tagcctggct caccgtggat accaacacca tactcgatct
     5401 catcttctcc tagttcgaat cctggtttcc ctacttctgg tacagttgct gctcgaagcg
     5461 ctagaccgat cgtcttagtt gcctgtacga ccttttctcc cagttctttc aactcagcta
     5521 gggaagcacc gtttcttgct gcatcgccca cgattttgtg tacaaggaca gtacctgcaa
     5581 ctccgcgttt tccagcagta tacgtactat tttcaacggc gatatcatca tctacgatca
     5641 cactttctac ttcaatatcg tccatagcag ctaattcctt tgccatatcg aagttcaatg
     5701 catcccctgt ataattctta acgatcagca aaactccttt tccactatcg gcttccttaa
     5761 tagccgtctg aatctgatct ggagttggag aagtaaacac atcaccaagc accgctgcac
     5821 ttaacatccc ttcccctaca aacccggcat gggatggttc atgtccgctt cctccaccag
     5881 aaaccaaacc aacttgttcc gctttctgat tcttggctac aacacgggaa gattctacac
     5941 gatgaacaag ttctggatag ctttttacta atccttccag catttcttcg acaatattgc
     6001 ttggatcatt catgattttt ttcaatttat tcccatcctt tctttccggc attttgtgct
     6061 tctgttaaga tttgctgagt atcatctgtt ataccggcag tgatggctgc tgcaaaagct
     6121 ccttccacta aaggcgcatc caccagatga taacgttgct gctgctcttc ttccaacatg
     6181 tcttttgcca gttccgagtt caataccgcg ctaccgatat ccgcaaaaac aaaataacta
     6241 gctgcatcgc ttgattcatt gactgcttct acgattttca tcgggtctga tccgatctcg
     6301 ccatttattc cgccgagaga aaaaatttgc acctcttcgg atttttgcat ctgctcaatc
     6361 atttctttaa taccatctgt tactttttga ctatgagaaa taagcaaaat actttttttc
     6421 acaagaatct tcctttcttt tacatagttg ctaacgattg gtttcgct
//
LOCUS       DACESX010000135         6419 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.136, whole genome shotgun sequence.
ACCESSION   DACESX010000135 DACESX010000000
VERSION     DACESX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.136"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..1175)
                     /locus_tag="IXF88_002348"
     CDS             complement(<1..1175)
                     /locus_tag="IXF88_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HAQ1509058.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFK"
     gene            complement(1189..2862)
                     /locus_tag="IXF88_002349"
     CDS             complement(1189..2862)
                     /locus_tag="IXF88_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1509059.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(2875..3192)
                     /locus_tag="IXF88_002350"
     CDS             complement(2875..3192)
                     /locus_tag="IXF88_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1509060.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(3217..4617)
                     /gene="lacG"
                     /locus_tag="IXF88_002351"
     CDS             complement(3217..4617)
                     /gene="lacG"
                     /locus_tag="IXF88_002351"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1509061.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4854..5639
                     /locus_tag="IXF88_002352"
     CDS             4854..5639
                     /locus_tag="IXF88_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1509062.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            5642..6334
                     /locus_tag="IXF88_002353"
     CDS             5642..6334
                     /locus_tag="IXF88_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1509063.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
BASE COUNT         1975 a         1454 c         1146 g         1844 t
ORIGIN      
        1 tgtttaaacg cacgcaagct tggcaaggcg tttccagcaa aatcaaacat cgcttggttg
       61 tcccattcgt ttcccatagt ggattgaaca ctgagataat tcatacctgc ttgtgttgcc
      121 caactcacat ttccgttgta ccaaagaggt tcccaataga agaagcccgc cccgcgatta
      181 ttcggtactg cctgaatccg atcggcaaga tccagcaaat actctgcttg tcctgtcggt
      241 gttggttcat atccccccgc agctgcttcg gcttcaccaa aagcatttgg catagtatcg
      301 gcattcgctg ttgtatgccc ataagctgtt tcaactacga tcacttcttt accataccgt
      361 tggctgatcg cattcatatt ggtttgaagt tcattcattg aaccgtgcca atacggataa
      421 tacgacactc caattaaatc aaatgacaca cctcgactcg tgatttcatc aaaccaccaa
      481 gtaaatgctc ctgtatcgcc accatcagct aagtggagca ttactggagt tgttttaggt
      541 tgagtgtctc gaacagcttg gataccagac ttcaaaaaag cagccaatcg atcaaattct
      601 cccccgcctt ctccccaaga cttgccataa ggccagagca ttccgctatt cagttcattg
      661 ccgatctgaa ccatatcagg ataaacgtcc agctgcttca tttccgacag aacgttcttt
      721 gtatagtcgt acaatgccgt attcatttct tcaaaggtca gaccttgcca tgctttagga
      781 agattctgtt tcccaggatc aacccaaaaa tcactgtagt gaaaatccag cagcacctta
      841 agtcccagat tttttgctcg ttttgcaagg tcaatcgtcg tttccagatc atttgtccct
      901 gctccataac tttctccatt actattataa ggatcctgcc acaaacgcag tcgaacatag
      961 ttgacaccat tattttttaa aatctctaag gcatcttttg aaatatcgtc ttcataatat
     1021 tttgcaccag atttttccat atctgacagg atcgaaatat ctgctccgcg aataaattcg
     1081 ctggcagatg cttcaacagt ttttgaaaca gcccccaaag cccctaacac agctgcaaat
     1141 aaaacagctt tacgcaacca aattcccatt ttcattatgg ctcctccttt attgaaattg
     1201 gttcagaata aactgaacag ctttttcagg atctctcgtt aatccgatat attctgctcc
     1261 ttttgtcgca accagcttga ttcctaagcg atcggtgtct tctttgatat catcatagta
     1321 agaattgact tgcggtgcta agaccaccat atcaaaatcc tgcagcattt cgtaatgaga
     1381 accataagca cccgccattg cactgatcgg tgctttcgat tcagatgcac ctttttttaa
     1441 agcattagct aacatcgcac ttgtccctgc gccagcacaa agtaccaaca cattgatttc
     1501 tttatttgca tcgctgataa cttgactcgc tgccacaggt acttcctcac gtacatcggc
     1561 agaagcacct tcctcggttg cgatcacctc tgctttttgt gcttctttct ctactaagac
     1621 agcatcgtat gctttgcaaa atggtagata gatcaggaag tcaacgacga ttagaaccgc
     1681 tgctagaaca aaagctaaga tacccatgcc cgttcctaga accaaaccga tcggtgctgg
     1741 agttgcccaa ggcaaaacgt acataaagct gttcatgccc aatacatcga caaaaaactt
     1801 gaacaggcaa acgtttacaa caggtgttaa caaaaatgga ataaaaaaat aagggttcaa
     1861 aatcatgggt gccgcaaata gcaatggttc gttgacagcg aaacttactg gaataaagga
     1921 agctttccca actgccttca actgcttaga cttagcaaag agtaggaaga taaaaggaac
     1981 aaccaatgtt gccccagttc ctcccatagt tcctacaaaa ttccctaaac ccaccgtcag
     2041 tactttagaa gcttgttcac cggaattgaa caattgcaga ttggtttcca cattggcata
     2101 gataattgcc gcaattgccg gctccacgat cgacggtcca tgaacaccga caaaccaaaa
     2161 cagcgccatc gctccccaga taatagcgat tcctaagtac ccgtcagctg ctgtaaatag
     2221 cggctgcagc accgtgatga ttgcttctgc aaatgagatt tggaagattt gtcgaatgcc
     2281 taaatcaatt aaaacggccg caaatactga aaaagagaaa ggaaaaatat ctcggaaggt
     2341 ttgcgaaatc gttccaggaa cttctttagg cattttaatg gtaatatctt tcagcacaca
     2401 aaacttatat aaattaactg ttaaaaatgc agcgacaaag gaagccagta gtccctttgt
     2461 ccccatatat tccgttgaaa atccaccttc aatcggagag acgcttaaca acaggaaact
     2521 taccacagag gccagcatca cagaggtggt gttgattact ttgtttttcg gtaagcgacg
     2581 attcatcgaa tcagccaagc agcgagcagt ggttgctgaa acgagtaacc ctacaacacc
     2641 catcgaataa ttatagactt tccatagcca tgtggagaca gattcaggcc attggtaacc
     2701 aaaaatctca ggaaccgcag caatcaaaat gaaaatgctt gaaaataaaa tagctggcat
     2761 cgctgttaaa aatccgtcac gaacggctcc taaataacta ttacgtgaga ttttttcaaa
     2821 aaatggtttc cctttttcga tttgtttaat gattccatcc atagcgttct tctcctatcc
     2881 ttgattctgt ctgtacagta caataaagtc ttgaatcaga tctctcagca ataaagtggt
     2941 cattaaatga tcctgcccat ggataaaaat gaatcccatc tccatctctt tcccagatgc
     3001 ttcttccgct aaaatttttg tttgcgaatt atgtgcaagg gttaaatttt cgtcggcttc
     3061 tttcaacgcc gaatcaacat gttcaaagtt tccttgtctt acttctttca ataatttcaa
     3121 taatgtactg cgagcgtcgc ctgaatatgc aacgatctca aaaccaagca tttgtaattc
     3181 ctctttattc atcttttctt cctccattat cacttattaa ataatcgttt tggtttcact
     3241 gaccaacttg taccagtaag ctgattcttt tgggtaacgc ttctgggttt caaaatcaac
     3301 ataaaacaag ccataccttt tgttataccc atttgtccat gagaaaagat ccataagcga
     3361 ccacaaaaaa taccccttca catttacccc ggctgttatc gccttgctta aggattccaa
     3421 atagactctc aaatagtcga ttctcggctg atccataata atgccatctt caaactgatc
     3481 tttatacccc atcccgttct cagtgatata gattttgttg taatgtgggt aatcgctttt
     3541 aattcgcagc aacaagtcat atagaccttc tggataaatc agccagtccc aatcagttcg
     3601 aggaattcct tctttataaa tacgttctcc gattcccttc actttataaa cagaggtccc
     3661 tttttcaccc gtaccgttat gatggattgc attttctcca tcgtaagctt tgacaaaatg
     3721 gcattggtag tgattgatcc ctaagtagtc attacgcgtg gatgcttttt tcaattcaac
     3781 gaaatcttct tcaggaaaat gatagctggc ctgatttgct tcacatatct catcaagagc
     3841 agtcaaggtt tcagttgaat aatagcctaa ataggtagca tctaataaga aacggatcga
     3901 caatgcatcg tctaaaaagg cagcatgctt atcttccggc gcgtctgttg ccgcatattt
     3961 cgtctctaat gagtgaacta caccaatttc acccggaagt tcgttttctt tgaaatagtt
     4021 cactacacgg gcatgagcaa ccatcatatt atgaagacag gcgacgatct ttgtaaaatc
     4081 atatttgatt cctggtggga aaacacctaa caaatattga ttggttgcaa ctggatagat
     4141 ttcattaaag gtactccaca ccttgacttc tttgaattca tgaaaacaaa aaatggcata
     4201 ggaaacaaat gcttctattg tttctcgatt caaaaaatct ccatggtcaa acaaacgttt
     4261 aggggtatca aaatgatgca acgtcacaaa cggcgttacg tttcttttct tacattcttc
     4321 gaacacacga tgataaaaag cgattccttc tggatttggt ttcccagctc catcaggaaa
     4381 aatgcggctc caagcaatgg ataatcttag accatttaca ccaaaacgtt cacacaattc
     4441 aatgtccttt gcatattgat gataaaaatc acttgccgga tcgggactga atcgcccttg
     4501 ttcctctaaa aagtcatccc aagcaacagc gcctttgcca ccttccttag tggctccttc
     4561 aacctgatag gctgctgtgg ctccgccaaa aataaaatct tctggtaatt taagcatgta
     4621 catctctcct caaatctata atcagttttc gctctttcta actgattgca ttgtaaacgc
     4681 atccaaaaca tttgtcaaca aaaacaaaca caaataatca taaaaaccag aaaacagcaa
     4741 taaaatctat tgtaacaaac ataaaaacca ccctaaatgt ttacttttgt tcgaattaga
     4801 ttgactgttg tccgtttata tgatagatta tccttaaatt agaggtgaag acaatgttga
     4861 aaaaagaaag attgcttact atcgtagaaa tggtcaataa aaaaggaatc cttaccgtta
     4921 atgaaatcat caatcaatta gatgtttctg atatgaccgt tcgcagagat ttagatgaat
     4981 tagaaaaatc gggcaagctg ctgcgtgtcc atggaggggc tcaaagtctt tcctacactt
     5041 tagatcaaga actttctcac actgaaaaag ccactctgca gattcaagaa aaaaaagaaa
     5101 ttgctgccac agcagcctcc ttgattcacg agggagaaac gattttctta ggaccaggta
     5161 caacaatcga actattggcg acgcatttac tgaacaagaa aattcggatc attacaaata
     5221 attatccagt ttttgatatt cttgctcaag cagatggacc agaaattcta ctgattggcg
     5281 gtgatttccg gaaaaatacc ggcgcatttg tcggaccgat caccaacgac aatttaagaa
     5341 gactaaagtt tacaaaagca tttatcagtt caaatggcgt tcacaacgaa gcgatctcta
     5401 cttacagtac cgaagaagga gaagcacagc agatcgctct aaataattcc cgtaagaaat
     5461 atctactcgt tgataataaa aaattcaatc gagatgattt ttatgtgttt tataatttgc
     5521 atgatttcga tctgctcatt acggatcgcc agatctcgcg ggaggttcaa gagcactatg
     5581 cacaatatgt cgatctgcaa attgctgatt caaccgaaaa taccactata ggagcgtaac
     5641 tatgaaaggg attatatttg atttcaacgg aacaatgttt ttggactctc atctgcatga
     5701 agctgcttgg ctgcatatga tacatgatca tacaaaagat ggtttatcag acgaggatat
     5761 tttaaaaaat attcatggtc ggacgaatac ggagattttg aaacatttca tttcagagca
     5821 tttgaccgcc tctgagatcg ccgtattgtc agaagaaaaa gaaagctatt atcgtatgct
     5881 ttgcttgcaa aatgaaaacg agcttcagtt gacaaaaggg cttgaagcga ctcttgacca
     5941 gctgactgac caacagattc cattaacgat tgccacggct actgtcaaag agaatgttgc
     6001 tttttatttt gatattttcg atttagcccg ctggtttgac tgggacaaga ttgtctatga
     6061 cgacggctct ttccctggga aaccgcaacc ggatattttt cttaaagcgg cagacaaact
     6121 gagcttagct ccggctgatt gtcttgttat cgaagatgct tactcaggat tacttgctgc
     6181 caaacgtgcg gggatcggaa caattattgc aattgatcca ttcggtaaaa acaaaacagt
     6241 atttgaacag gcacagctaa ataaagatgg aaccatcaaa gattttactg gtttttggac
     6301 agagcacctg tctgccacat ccgttaattc ttaaacgatt caactaacta ccaaagaaaa
     6361 gcaactgaaa tgcacacctc cttttataaa aaactgccag tgaaacgaca tgaaagata
//
LOCUS       DACESX010000136         6409 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.137, whole genome shotgun sequence.
ACCESSION   DACESX010000136 DACESX010000000
VERSION     DACESX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.137"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(296..1024)
                     /locus_tag="IXF88_002354"
     CDS             complement(296..1024)
                     /locus_tag="IXF88_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1509064.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="IXF88_002355"
     CDS             complement(1002..2576)
                     /locus_tag="IXF88_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1509065.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="IXF88_002356"
     CDS             2805..3743
                     /locus_tag="IXF88_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1509066.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="IXF88_002357"
     CDS             3755..4666
                     /locus_tag="IXF88_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1509067.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="IXF88_002358"
     CDS             4690..6162
                     /locus_tag="IXF88_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1509068.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..>6409
                     /locus_tag="IXF88_002359"
     CDS             6213..>6409
                     /locus_tag="IXF88_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1509069.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKI"
BASE COUNT         2041 a          952 c         1242 g         2174 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagtgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatcta
//
LOCUS       DACESX010000137         6388 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.138, whole genome shotgun sequence.
ACCESSION   DACESX010000137 DACESX010000000
VERSION     DACESX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.138"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(279..3428)
                     /locus_tag="IXF88_002360"
     CDS             complement(279..3428)
                     /locus_tag="IXF88_002360"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ1509070.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3441..4658)
                     /locus_tag="IXF88_002361"
     CDS             complement(3441..4658)
                     /locus_tag="IXF88_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1509071.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(4648..6243)
                     /locus_tag="IXF88_002362"
     CDS             complement(4648..6243)
                     /locus_tag="IXF88_002362"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1509072.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1784 a         1317 c         1056 g         2231 t
ORIGIN      
        1 gtttcatttc agatacattt cacttaagcg gattccaaaa aaacaaaaga aaaaggaacc
       61 cctagttagc ctatgctttt tattggttcc tctcaaaaca cggtcatata acaacggggt
      121 ggggtaaatg aatgaaaaag gtggaggaaa cattattcaa tagctgtctt aaatacttcg
      181 ttataaaatg tgaacaaata aaagacatct atccctattt tctacacttc cacttgacta
      241 ccatctttga atcgtacaat tttcttaact cttctcgttt acctcaattc attctcaagt
      301 ggaatcaact catcatctaa cattttacgc catgctcgct tcatttcttg tgggtattta
      361 aatggcttcg cttctggatg tttggcttta tatccttcgt aatccttact gttgataatt
      421 gctttcatat taggaattgg atcttcacct ggtagatatt gcagattaga agcatgtaat
      481 tctttctcag aaacaaacca ctcattggaa aagacatgaa tcaaatcatc actcgcttgt
      541 gtaaaatagc gacgtttaat gacaaagata tcttcattac tatctaaagt ccccacatca
      601 aatgcattta aaatttgtcg gtaaacttct ttgactcctt ctgttttatt caatttagcc
      661 aaagcttttt caatttcttc tcgtgcatct gaactattcg cagcataagt tcctagtaat
      721 tgatcaatat aagttgaatc aatatcatac aacttagtgg tattttcact ataaaattcg
      781 atcgttgaaa aatcttctcc ttctttttct ggttcttcct ctattaatga acccttcacg
      841 gtctcgtaca cgccaatttg ttcttccaaa agttgaattt gattgttcaa agttggagat
      901 ttcaccatgt catcattgta atcgtcataa gtcactaacg attcataagc acgattcaat
      961 tcttgaaatg ctttgacgta ctcgatacgg atttcaagtg gcgtatgttc atcaatggat
     1021 tccatggatg aaacctcttt taacttacca tatgcttgtt tgaatctttt tttggattcc
     1081 tgataacttg gataaatcaa acctgattct tcttttgctt ttgaatacag ttttgtcgca
     1141 tcctcgacat tcttctccat tgtcgctggc ttacgaaacg tcacaatcaa tccttttgtc
     1201 ttatcaggat acgtacgatt cgtacgtgaa aaagcttgga tcaatcctgc atactctaga
     1261 ttacgatcca caaatagggt ttgaatcgta ggggcgtcaa aacctgttaa tagacgatcc
     1321 actacaatcg ctaaatcgat ttgtttccca aattgtttga attccgctct ttttcgtgcc
     1381 aaacgattat tgatgtctcc gttataacgc tcaatgtctg ctaatgacca ggaagtatca
     1441 taatactgat tatattcctc aataattttt tgcatttctt cttgttgggc acttgaatct
     1501 cggctatttt cttctaatga ataggtaata gcgattcgtg gaaaatcagg atcctccatc
     1561 gttcgtcctt cacgaatgag ctgattggga aactcttttg ttaaccaatc aggctctttc
     1621 gtcatctctt tgatggcccg ataatacttt ttggccatgt caatggaact cgtggttaag
     1681 attgcagatt tcgttggcct gccatttcga aaatcaaatt ttgtataggc attatctgga
     1741 cggaacattt tatgaatgac tttttggata tgttcatctg cttcaaaaac agctgggtcc
     1801 aaataacttt cttttttgac aggttccatt tgatcaatca tacgattgat ctgttctgat
     1861 gaatactctg catatgtttc tacttctttt aatttccgat agattttatt ctctagtgaa
     1921 gtcggctcca cggtgttttc atgctctact tgaaatccta agacagagcc atcttctagc
     1981 gcattcttaa tggtataggt atgcaagacc tctccatact gatcatgggt tgtacgtgcc
     2041 aattgccctt tggcttgttt ccgattctca ggaaaaatcg gtgtacctgt aaagccaaac
     2101 caagtggatt tcggaaacat ttttttgatt tcttccatat tttcagcact taacgctcga
     2161 tgacactcat ctactacaaa cacgatatgt tggcccatta atttttggaa gcgattggtt
     2221 ccttttttct cttcttgttt ctttgcatat ttcaacgccg catctagttt ttgacgggtt
     2281 gtaatgatta caacatttgc attggcatct gccaacaggg tttcacttaa ttctttggca
     2341 cttcccgttc caacgatcaa gctattcgct ttggcgttcc cggaagaaat cccagtatta
     2401 aattccgaag caaattttgt aaactctgtg gtggtttgat tatctaaatc tttgcggtcc
     2461 actaacatga tcgttcgatc gatccccgat tttcgtgcta aaagcttagt agatacaaaa
     2521 ctagtcaacg tctttcctga accagtcgca tgccacacat accctgattg atgtttattt
     2581 gcggcaataa acaaggcttg aatcgcatgt acttgataag gatgtaacac catgagggtt
     2641 ttattgtctt gatcttcact gacgattgta taatccgcaa tcaaacgatg agcatccggg
     2701 atatttaaca cttgtttaca aaactcatat aagttttcta ctttttcatt gtctttcgtt
     2761 cgccaactaa ataaaaactt agggtgcaag tctttcggta aggcatttgc aaaataccga
     2821 gtggtttgtt cattggaaac cacaaacaac tgtaaggtcg aaaagatatt gttccgaaac
     2881 ataccttctt ctgcatattt tttgatttgg ttaaatgcct gataaacacc atcttttgca
     2941 gttacttgtt tcagctcgat ttggacaatc ggtaatccat tgattaataa ggtgacgtca
     3001 aaacgtcgat cccgtgcttc tatgttactt ccacgttttg caatttgatg gaccacttca
     3061 taagtagaga ttcctccacc aatgtcttga ttggaataaa ggataagtga gacagaaccc
     3121 aattgcgaat cttcccgttc aatcgtgatc cgagccatcc cattttcacc ttttaaccat
     3181 ttcgcagcat caaatggggt tttcgttcgc agcagtaatt ccgttttgat cgtttcaaat
     3241 tctttatcgg tcaaaggaaa atcattcagt tctgcttgat tatttgagat aattttttgg
     3301 cgtaaattga cccaaaggtc ttcttctgat tttagatccg gacgatacgt ccactgatta
     3361 tgcccttctc caagtacacg aatcaactgt tcttcgactt ctgcttcctc acggtgacta
     3421 attttcattc ttcttccctc ctagacaaac atcttttgta agaagctttt tttcgtttct
     3481 tttagtaaat ctaacttacg ttgatgaaga gcgatcgtat catctagctg tttgaagaaa
     3541 gtaccgattt tttgttgttc atcctttaca ggaaccttaa cactgatatt ctcaatatca
     3601 gaactgttat aagaaggcaa tgcccctact tgggagtatc ttggcaaatt tatagtttca
     3661 aacaatattt taccaaaata gatatcccaa gtattattaa ataaataagc catggtattg
     3721 ttatctatta agaaagggct tgtgaccaaa cgttttctat taagcattat tgctgcgcca
     3781 actttagcaa atataatcgc tggcacatct tttattggct tccatttttt acgctcaatt
     3841 tgctcattag atacataatg atttgcattt ctcatttcat actcattacc aatgttattc
     3901 atgtcggaaa ctttaaaaaa tggagtacct tcagaaccac cttgctcggc atcaggaaat
     3961 ccaattcccg attgagtttt acctaattct cccaacttac gctcttccca atcttccgta
     4021 aaaccaggaa accggatttc tggcactttt gctccgtttt cagggaacat tttttgtaag
     4081 aatccttttt tcgtttcctt cagcaaatct aacttacgct gatgaagagc gatcgtgtcg
     4141 tcaagttgtt tgaagaaggt gccgattttt tgttgttctt cctcactagg aaacattgct
     4201 actatatttt taaacttttt tagagacaga acatgttgac ctgttccctc tgcttgaata
     4261 gataattgtc gttggattcc attgttaata cgtcttaaga agaagtcgga atcagcagat
     4321 tttaaagtaa atacaggata aaaatagctt ataatccctc tatcaataat gtcattacgg
     4381 ttaaacacaa acacatcatt atcacttcga ctacggaacg tgaaatatcc tctaggtagt
     4441 acaaagtaac caatattatt ttctgtagtt acttgccgat ttgcaaaata gtcttcttgc
     4501 aaaactatcc ctttttgttg agatgaagtt agcataggaa attgattttg tacacttgtc
     4561 ttttcaacat aatctacaac gaagtccccc aacttcctct gttcccaatc atctgtaaat
     4621 cctgggaaac ggatttctgg ttgtgtatca ttgctcatgt ttgaacacct ccagcgcacc
     4681 ttgtaaccac gcttcatttt caggtgttgt ttgcagggaa gagatagctt ctaaaagttt
     4741 tttttctagc acttgctgtt cttgtcgtat cttcttgatg tcatttccga catgaaccat
     4801 atcaattggt tcttcttctt cgaaagtatc tacatagcgt gggatattta agttatagtc
     4861 attttctttg atttcatcaa aggttgccaa atgagcgtat ttctcgacat cttttctttc
     4921 agcgtaggtt tcgaggattt tttgaatgtt ttcttccgat agtttatttt gatttttccc
     4981 tttaacaaat tcacgactag catcaatgaa taatacatct cgagtttgac gattcttctt
     5041 caacacaatc accgtcgttg gaatcgatgt cccaaagaac aagttcgcag gcataccgat
     5101 cacagcataa atactgccat cttctagtaa tttttgacgg atgacccctt ctgcagcgcc
     5161 acggaataaa actccgtgtg gtaagacgat ggccatggta cctgtttcct tcaaatgata
     5221 gaacccatga agaagaaacg caaaatctgc tttggatttt ggcgctaatt tcccataacg
     5281 attaaaccga gaatcatcta agaaagtcgt atctgctgac caattagccg aataaggtgg
     5341 gttcattact actgcatcaa atgtgtaagg ttcatctgtt ggccagtcct tattcaacgt
     5401 atccccattg cggagattca tttcttcggc gtcgacacca tgtaaaatca agttcatttt
     5461 tgctagattg tatgtcgttg tatttagttc ttgcccatga tatttaacgt tatctgggtg
     5521 tgttaagtag ttacgtacgt ttaacatcaa tgaacctgaa cccatcgttg gatcaaaaac
     5581 actaaagaat cgtcgttcct tttgatcgag cgttacgatt tgcgccatca tatcagaaac
     5641 catatgaggt gtgtaaaatt ctccggcttt cttccctgct tctgaagcaa attgactgat
     5701 cagaaactca tacgcatccc cgatcacgtc cccatcgtgt tctaaaacat ctacatcatt
     5761 caatttcttg atgacttcgg taatcgtcac atttcgttgt tgctcatctg ttcctagttt
     5821 ttttgattgg agatccacat catcaaataa cccattaaat tgattgtagg tagatgccaa
     5881 ttgcacaaag gctttgttta gatcgttcaa ttggaacgtt gcttgttttg cttgatccgc
     5941 taagacgttg aaaagatact ctggtgcaat tgcatatccc aaaatatcaa cgattgtggc
     6001 aatcagatct tcttttgatt cttcatctga aagtaattca cgatacaaca ttgtttgttt
     6061 gcttactgtg tcatattcct ctaaggattc atctgctaaa agaaccactt gttccaacaa
     6121 tcgatcggat aaatatttat agaaaatcag tcccaataaa taatttttat attctgacgc
     6181 atccatttta ctacggagat tatctgccgc actaaataat ttttgattta attctgcacc
     6241 caaaaagata cactcctatc tcttatttaa agttgctata taccctctta ttttagcata
     6301 ccccctttta taaaaagcaa aaaaaaaaag aaagaaactt attcttgcta ataaactacg
     6361 cgaaaggagt ctaacgttgt ttgatata
//
LOCUS       DACESX010000138         6263 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.139, whole genome shotgun sequence.
ACCESSION   DACESX010000138 DACESX010000000
VERSION     DACESX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.139"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            936..1277
                     /locus_tag="IXF88_002363"
     CDS             936..1277
                     /locus_tag="IXF88_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1509073.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNEWFKMTLTNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            1391..1630
                     /locus_tag="IXF88_002364"
     CDS             1391..1630
                     /locus_tag="IXF88_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509074.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            1742..1936
                     /locus_tag="IXF88_002365"
     CDS             1742..1936
                     /locus_tag="IXF88_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509075.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            2022..2693
                     /locus_tag="IXF88_002366"
     CDS             2022..2693
                     /locus_tag="IXF88_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1509076.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            2746..4722
                     /locus_tag="IXF88_002367"
     CDS             2746..4722
                     /locus_tag="IXF88_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1509077.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            4737..5489
                     /locus_tag="IXF88_002368"
     CDS             4737..5489
                     /locus_tag="IXF88_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1509078.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            5505..6260
                     /locus_tag="IXF88_002369"
     CDS             5505..6260
                     /locus_tag="IXF88_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509079.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
BASE COUNT         2292 a          935 c         1345 g         1691 t
ORIGIN      
        1 ttttgagtct cgaagatatt tgtcactacg gtaaacggtg taccgctaca gagaaaatca
       61 cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc
      121 agaaagacaa agtctcagag gaaatgattt attacgctgg taaacaaaag caaataattt
      181 taaaagatcc tatcccttta aaagaactat atccaacaat caaacatgtt tatgaccaaa
      241 acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg
      301 ggcgtttaat cagctgaatt gatcattctc ataacttgta ttttatcgtt tttcgcttgc
      361 ttgaaaatat tgtcgagact tctaaaagaa cttagaatta ctagaaaaat aaggagtaga
      421 cttgatctat caacaattcc tttcgggggg tgacggggta ggtttttacg attggtaacg
      481 taaaaaattg gtcaatcgag taaatgccct tgtttggata gtggtgaaat gaatttgtgt
      541 aaataatatg cacagagtaa acaagaggga atttagaagt tagacaagtt agaatagcgc
      601 ctagattaat catgagcagc tactaaggga tctaggcttt gacgcaaact catggaataa
      661 ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac
      721 atgtagatac ttgcgaggac gttggtcatt ggatcaatcg agcatttacc gatttctact
      781 agggtttctc gtttcggacg ccataaacga gtcgatattt tttatgtatt tttaagtaaa
      841 cgaaaaacga taaaatatga acgagtcctg aataaagtga aaaaattgat ataaaacata
      901 gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat ccgaatatac
      961 gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa ggatttgtga
     1021 cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattagac gaggatgatt
     1081 tatactatca aaaattgact gataatccaa ttggattcgg cttgctacct gaagatgatg
     1141 aaggtaatga atggtttaaa atgacgctta cgaatgataa tggagacgaa ttatctgttg
     1201 aagatacttg gtcatatttg agtgattata ttgtcagcgt tgaaatcatt gaatttgtag
     1261 cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct tagaagaagt
     1321 cacgacgaat aataaaaagg tagtattacc ttaggcattg agtaaggaat tcatttagga
     1381 ggaaaattag atggcgcgac cagttttaga ggcgatggat atggatactt tgacaaaagg
     1441 aaggtatgta cagctgttag ctcgtggtta ttcacctaaa gatgtattca aatccctgtc
     1501 taaaggaaca gatatggaaa aagaaagatt acgaaaagaa ttcgattatt ggagaaccca
     1561 taatggaatc aaggatttga aaccagcacg gccagtaatt aaacgaaaga aaaagataac
     1621 ccaagactga agtgtagagc gtatgtgatc ataggctttt ttatttttgc aaaaaacaag
     1681 gaaggtagga aaactcatga attatgaaga aatcgaaaac agaaaaaaag tgtcgaaata
     1741 aatggaagaa aagttgctaa aaacgatgaa acaaaaacac cttaaaagat tatctgtcat
     1801 gcaatatatc aatgatatgc agataacagg aaaagagaaa gcttgtttgt taggttcaat
     1861 gaaaaacttt gaacagttga gacgaactta tgtgaaaaca agttctaatt gtcaactatt
     1921 gttagaagtc tcatgacatt cattaataga attcattttt aaaacttttt ctatggaaat
     1981 gagaaaaaga ttttttgttt tgtctgaaag ggggtagctg gatgagaaaa tggttatttg
     2041 gttttttagg agtcgcttta atcgtctttt gcagtgtgtt tggctatgtt agctaccaaa
     2101 aacatgaagg agaggtgttt aagcaaaata tagaaaaaaa gatgcccgta gatcaaatca
     2161 atgcacatgc aaagagttat aaggaggatg caacgaacgt caataatgat atgtctttgg
     2221 ggcaaatgtt gtccatacaa caagaagcaa tcgaaatggg cgtcaataaa caagtatttg
     2281 cacaaattca gattcctgca cttggtttag cattgccaat ttttaaaggg gcaaatcaat
     2341 acactttgag tcttggagcg gctacttatt tttatgaaga tgctgaaatg ggcaaaggaa
     2401 actatgtcct agcaggtcat aacatggaaa tgccaggtgt tttattttct gatattcaaa
     2461 agttgtcatt aggggaggtg atggacttag ttagtaacga tggcgtctat cgttacaagg
     2521 taacgcgtaa atttatcgta cctgagtact ttaaattaat cgatggcgtt cctgaagaga
     2581 actctttctt gtctttgcca aagaaaggag agaaaccctt attaacgttg tttacttgtg
     2641 tttatacgtc acaaggaaaa gaaagatacg tggtacaggg agaactacaa taacaaggtt
     2701 ttaatcaacg tgagtcgttg aaatatgaaa tgaggagagt aaaaaatggc aataaatcgg
     2761 tatgtaaaaa aagctggaat gcttattgga atgttaggaa tcattgcagg ctgctttcag
     2821 ggatatcgag catatgcaga agatgtcacg caaaaaacgc caccagagaa ggtcaatatc
     2881 acggttcata aattaatgta cgatcaagga acgcaattga atgttgatat tgacgggatc
     2941 aaaaacgatg gttatacgca cgatcaatat cctacaggtg taacgaaata caacaaggca
     3001 gattatggtg atgttgagtt tacgttagga aatattacgg atcaagtatt accgagagaa
     3061 gattctgatc taaccaacgc gaaagtggac gagattgtac aagatgtaga agacaaggga
     3121 tctaacagtg agtacgtcaa gaatgcaacc aacaaaatta cttcagctgt agatgaaaat
     3181 ggtgagatca cttttactga tcaaccagcc tatgtcaatt ccaaagggaa tgtatatgtg
     3241 gtttatgaaa gtaaatccgc tgctggatta gtcacacaaa aagcaaaacc aatggttgtg
     3301 attgcgccaa tgacagataa aacaggatca ggatttttaa aaaatattca tttgtatcct
     3361 aagaacattg tttctaagtt gagttttgaa ttaacgaaat ttggtgacga tggcgcagac
     3421 caaagcaaac aaaccctttt aaaaggggca aaatttgagt tatacaaggg cgaacctgga
     3481 aaaggaacaa aattgggtga tcttgtatcg gatgatcaag gaaagctgac tgcgactgat
     3541 ttgacgttag gaaaatacta ttttgtggaa gttccttcgg aagttgtagt gggatcagat
     3601 caagaaccaa cagcagatca atatttatta ggcgcagacg ctcgcaacga tgcgcacaat
     3661 aaattaacct ttgaaattac gaatgatggc gtgacaagtg acttaaaagc ttcatacgta
     3721 aactataaag caccagtgat cgataaaaca gtaacaaatg gaacaggaca agaacattct
     3781 ttccaaattg gtgacgcagt aaattatcaa ggaacgattc atattccaac agacattgca
     3841 ggtggcgcag acggtatcac agtaaacggt gtgaaaagtg aaacttctcc ttacagtgta
     3901 tttaaatggg gagatacagc aggacaggga ctttcttatg tcgctgctaa agcaaatatc
     3961 aaagtaacaa ataaagatgg atctgtggta ttaaaagaaa atacagatta taaaatccaa
     4021 aattcagaaa atggctttgt gatcgatttt atcgtgaata atggccaagt aagtgatact
     4081 gtagcaagtt tacacggaca agatcttcaa atgacttata acatgtatgt aaatgacagc
     4141 gcagctgttg ccaatccatt aacaaatagc gtggatttcg tttataacaa caatccattt
     4201 aatcaggaag aacaccacga aaaaacgaaa gctgacgttg tgacttatgg tgcaaaattc
     4261 ttaaaagtcg atagcggact atttggaaca ggaatcaaag caacaccgct tgaaggcgca
     4321 gaatttgctg ctaaaaacgc agaaggaaaa tattatggtg gattagttga tacggataaa
     4381 gacggtgtca aagaagcagt atgggtagac gatgtagcaa atgcagcgat cctaaaatca
     4441 gataaagaag gtcactttga aatcactggt ttaactgaag gtgaatattc actggaagaa
     4501 acaaaagcgc ctgaaaacta tcaaaaatta acgaaagaga tctctttcaa agtagacaaa
     4561 gattcataca aagaagaaaa ccgaatcacg atcaaaaaca atcaaaaagc ttccgtacca
     4621 atgaccggta gcaacggttt tcaaacgtat gtattaatct cttgcttgtt gctaggagca
     4681 ggggcacttt ctgcagttgt ttactttaag aagaaagcct agaaggatca aaaccaatga
     4741 ttatcaggaa gcctaagaaa aagcgaatca tgggtaaatg gatcattgct ttctggttac
     4801 tttcagctgt aggtgtctta cttttgatgc ctgctgaagc gagcgtggcg aaataccaac
     4861 aaaatcagca gattgccgca atcgatcgaa caggaacggc agcagaaacg gattcttctt
     4921 tggatgttgc taagattgag ttaggcgatc ctgttggtat tcttacaatt ctaagtattt
     4981 ctttaaaact accaatttat gacggaacgt ccgacaaaat tttagaaaat ggtgtaggta
     5041 ttacggaagg aactggtgat attacaggag gaaacggtaa aaatccttta atcgcaggtc
     5101 atagtggact ttataaagat aatctttttg atgatttacc ttcagtaaaa aagggagaaa
     5161 agttttatat caaagttgat ggtgagcagc acgcttatca aatcgatcgg attgaggaag
     5221 tgcaaaaaga cgaactacaa agaaactttg tcacttactt agaatcaaat cccaatgaag
     5281 atcgagtaac gctaatgaca tgtacgccta aggggatcaa cacacaccgt tttctagtgt
     5341 atgggaagcg agtaaccttt acaaaatcag aattaaaaga tgaagaaaat aagaaacaaa
     5401 aattatcttg gaaatggctt ttaggaagta ctgtttttct ttcagtcatg atcattggtt
     5461 cgctatttgt ttataaaaag aaaaaataaa ggagggtaac caacatgaga aatagaaaat
     5521 ggctatttgt ttgtgcagcc gctctttttt ctttcactgc ctacacacag ttgacgtttg
     5581 cagacagaca agaaagcacg gatagcacag aacaaacaat ccaaacgtca gatagcagca
     5641 cgattcaaag cagcgatgaa accgtcgatt cgtcaacagt tgattcttca gaagaaccta
     5701 aaattgctga agacgatcaa atagtcaaac agaccattca cgttcaaaag attatttctt
     5761 cagaacaatt agacaaccag gggcatgaaa aacaagaagg agtaaatggc gcgaagtttt
     5821 ctgtttatga tgtatcggac attttacaaa agatggatgt taaggatctc actactgacc
     5881 aaattgaatc ccaattaaaa aatcgagtga aaaaactatc cagtgatcaa ttgaagctag
     5941 tttctaatga agaaacaaag acaattgatc aacaaacagg tgtctttgaa ttctcggtag
     6001 aagtacaggc aaatcagaaa caagcttatt atattgtcaa tgaatcaagt cctgaaaaca
     6061 tttctaatag cgaggatatt ttgctattaa cacctgtttc tgataaaaac ggtgaattct
     6121 taaaggatgt gtggatctat cctaaatcag aagcttcgca acctaaagaa gaagtaaaaa
     6181 aaatcgtttc tactggcgta aagaaaaact tctttgagaa ttgttgggat tttgttactc
     6241 acttgttttc tagagattaa gga
//
LOCUS       DACESX010000139         6253 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.140, whole genome shotgun sequence.
ACCESSION   DACESX010000139 DACESX010000000
VERSION     DACESX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.140"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            7..525
                     /locus_tag="IXF88_002370"
     CDS             7..525
                     /locus_tag="IXF88_002370"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1509080.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            653..1336
                     /locus_tag="IXF88_002371"
     CDS             653..1336
                     /locus_tag="IXF88_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1509081.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1645..1718
                     /locus_tag="IXF88_002372"
     tRNA            1645..1718
                     /locus_tag="IXF88_002372"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1679..1681,aa:Arg,seq:tct)
     gene            1877..2236
                     /locus_tag="IXF88_002373"
     CDS             1877..2236
                     /locus_tag="IXF88_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1509082.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2359..3990
                     /locus_tag="IXF88_002374"
     CDS             2359..3990
                     /locus_tag="IXF88_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1509083.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4052..4693)
                     /locus_tag="IXF88_002375"
     CDS             complement(4052..4693)
                     /locus_tag="IXF88_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1509084.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4975..5283
                     /gene="rplU"
                     /locus_tag="IXF88_002376"
     CDS             4975..5283
                     /gene="rplU"
                     /locus_tag="IXF88_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1509085.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5310..5654
                     /locus_tag="IXF88_002377"
     CDS             5310..5654
                     /locus_tag="IXF88_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1509086.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5667..5960
                     /gene="rpmA"
                     /locus_tag="IXF88_002378"
     CDS             5667..5960
                     /gene="rpmA"
                     /locus_tag="IXF88_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1509087.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT         1934 a         1042 c         1320 g         1957 t
ORIGIN      
        1 aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt gattatattt
       61 ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac tccttatatt
      121 attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata tgtacgaccg
      181 aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa ttcaggaaaa
      241 aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt ttatacaaaa
      301 gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat tatcggtagt
      361 cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt tgcatggatt
      421 caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg gaataccttt
      481 gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg ttaaaaacat
      541 aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa acactcatgc
      601 agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta gtatgcgcaa
      661 tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc gtacccagca
      721 aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag cagaagtacc
      781 agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc ttcagcctga
      841 acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg ccacgacaat
      901 cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg agaaagctaa
      961 aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag gtcaagcagc
     1021 agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata gtgcaaaatc
     1081 aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg gcgttttgat
     1141 ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga ttccccgtgg
     1201 caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg atcatcccga
     1261 tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa aggaatcaga
     1321 aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg aatcgagggc
     1381 tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg atacaggaaa
     1441 tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt gataacctta
     1501 gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg gccaaaataa
     1561 ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta attatctata
     1621 attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc atccgccttc
     1681 taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt tagaaactga
     1741 ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca aaggcaaata
     1801 aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa aactcatcag
     1861 aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag ttgcaattga
     1921 atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga caagtagggt
     1981 ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt ttaagaacca
     2041 aggagtgtac aatggactat taggagtggg actactttat ggagcttatc tttcttccgc
     2101 atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat tatatggcag
     2161 tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt tggcattgct
     2221 ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa ctttgtgaaa
     2281 aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag aaaatttcat
     2341 gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa agaattttca
     2401 ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt agctgctgta
     2461 gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc cgcaggtttg
     2521 attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt ttatcaaata
     2581 tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac aaagggaatg
     2641 caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc aggacttttc
     2701 cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt tacttcaggt
     2761 attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca ctcggaaggt
     2821 acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat aaacacagcc
     2881 tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa aaaatgggcg
     2941 aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat gctattgttc
     3001 catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag taatcgtttg
     3061 aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc tattagcatt
     3121 gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg aatggcaaat
     3181 aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct gttgcttcct
     3241 ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc catcaaatct
     3301 ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct ctccatgctt
     3361 gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt tttgattgtg
     3421 acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa taaatattgg
     3481 tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga tttaagtatt
     3541 gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag cgctgctatc
     3601 acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac ggaaaagctg
     3661 aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc agaaaaactc
     3721 aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat gcgaggcgta
     3781 ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa agcagagcaa
     3841 agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca actttctcac
     3901 ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt gacagcttta
     3961 catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac aagactgtaa
     4021 actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt gaagctagta
     4081 cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata gctgctggat
     4141 gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc ttttgttctg
     4201 ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc gtatattctt
     4261 tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt ggctggacct
     4321 tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga gtaggtgtcc
     4381 gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga gtcacgcgat
     4441 acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag ccagttgaag
     4501 aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc gtatcaatct
     4561 ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt ccttgcccag
     4621 cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt atcagttctt
     4681 ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa gaaacacaat
     4741 gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg cttgaatttt
     4801 tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg taagctagta
     4861 ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa gcacagcata
     4921 tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta tagcatgtac
     4981 gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc aatctatgtt
     5041 gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat cttagtaggc
     5101 ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga aggtactgtt
     5161 gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa aaaacattca
     5221 caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc gatcaacgct
     5281 taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag cgaaatgacg
     5341 caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct tatggaagtg
     5401 atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg attgcctcat
     5461 tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat ctttataccg
     5521 aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta ttagaaaatc
     5581 ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa attaaaacaa
     5641 ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa ttatttgccc
     5701 acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa cgtctaggtg
     5761 ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt caacgcggta
     5821 caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta tttgcaaaag
     5881 ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg tctgtttatc
     5941 cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca agacttttgt
     6001 cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag tttactttgt
     6061 ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat aactatcgtt
     6121 ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg taaacagtgt
     6181 tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt taaattgaac
     6241 atttctatct atc
//
LOCUS       DACESX010000140         6243 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.141, whole genome shotgun sequence.
ACCESSION   DACESX010000140 DACESX010000000
VERSION     DACESX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.141"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(45..233)
                     /locus_tag="IXF88_002379"
     CDS             complement(45..233)
                     /locus_tag="IXF88_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1509088.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            503..865
                     /locus_tag="IXF88_002380"
     CDS             503..865
                     /locus_tag="IXF88_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1509089.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            917..2599
                     /locus_tag="IXF88_002381"
     CDS             917..2599
                     /locus_tag="IXF88_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1509090.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2716..4752
                     /gene="recG"
                     /locus_tag="IXF88_002382"
     CDS             2716..4752
                     /gene="recG"
                     /locus_tag="IXF88_002382"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1509091.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4801..5802
                     /gene="plsX"
                     /locus_tag="IXF88_002383"
     CDS             4801..5802
                     /gene="plsX"
                     /locus_tag="IXF88_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1509092.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5924..6166
                     /gene="acpP"
                     /locus_tag="IXF88_002384"
     CDS             5924..6166
                     /gene="acpP"
                     /locus_tag="IXF88_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1509093.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2075 a         1087 c         1425 g         1656 t
ORIGIN      
        1 aataaaaaac cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact
       61 tttcctgatt tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata
      121 acgcgaactt tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa
      181 cggttgtttc cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt
      241 tcctcctttg ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc
      301 atatagaatg ggaatttgca agtacatttt catagataca attgacaatt gagaagaata
      361 attgtaaatt aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa
      421 ctgcctttta tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt
      481 tacccgttaa ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga
      541 aattaccaat gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt
      601 tgtaggaatg gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga
      661 aaattactca agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta
      721 tacgattatc agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt
      781 gaaatacaat ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca
      841 aggtgttcgt gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg
      901 aaggaggatt atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt
      961 ccaggcaggt gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt
     1021 ccctgtgcca gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa
     1081 ggcagtagta gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg
     1141 attgctgatg ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt
     1201 ttctaagcaa attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac
     1261 tcatggagtt gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac
     1321 agtagctaga gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt
     1381 tgaagtaatg acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt
     1441 gcttccagtc ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt
     1501 ttatgagggc tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc
     1561 ttctccagca gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca
     1621 aatggcgaca gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga
     1681 aggacctaca gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat
     1741 aggagattct ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga
     1801 aaacccagga gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt
     1861 cgacaacatg cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt
     1921 tgcgaaaaaa gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg
     1981 cgtccaagag ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat
     2041 gaatccaagt acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat
     2101 tgtcttgcca aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga
     2161 tatcccagta gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc
     2221 attcaatgat caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag
     2281 cgttgtgagc ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat
     2341 caaagaagga gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat
     2401 cacagcaaca gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac
     2461 gatcttggca ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac
     2521 agaaaaacat cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta
     2581 tctattttct gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat
     2641 aaaaggcctt cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg
     2701 agggaggaaa aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga
     2761 gaaaaaagag cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact
     2821 tattatcctt ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa
     2881 gaaaaggtta ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat
     2941 aaaaaaagcc gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt
     3001 tttaatcaac cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt
     3061 aaatgggatg caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg
     3121 gatgactttt cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat
     3181 ctaatcaaaa aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac
     3241 cttgtggaga aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca
     3301 aaagaccacg aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta
     3361 tttcaaatgc gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag
     3421 atcctttatg atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca
     3481 aatgcacaaa aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg
     3541 cagcgacttt tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta
     3601 tacgctaccg ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca
     3661 caacaacata tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg
     3721 attacggggt caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg
     3781 gaaattaacg tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat
     3841 ttaggtctag ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt
     3901 cgtgaaaaag gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca
     3961 ttagctatta cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga
     4021 cgtatcccga tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg
     4081 acgaaaaaag aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa
     4141 tcagaagcat tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt
     4201 gcaccaaaat atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta
     4261 atcatggaag ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa
     4321 gtaggggtaa acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga
     4381 ttggcacagc tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt
     4441 atcttagttg ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag
     4501 accaccaatg gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta
     4561 tttggcttcc gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga
     4621 aatgttttgg aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag
     4681 atgcttcctg agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt
     4741 tatttcgatt agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca
     4801 atgaaaattg ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc
     4861 gtggctttag cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca
     4921 gaaatcaaga aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa
     4981 atcaatagtg atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc
     5041 cttgctgcac aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca
     5101 ggcgcgctac tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct
     5161 ggcttgatgt ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat
     5221 cttggcgcta acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct
     5281 ttttatgcac gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga
     5341 acagaagcaa caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa
     5401 acaagcttga attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat
     5461 gtagtggtca cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca
     5521 atgaatatga tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg
     5581 ggcgctatgc ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa
     5641 catggcggtg ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca
     5701 ggccccgatg ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta
     5761 gttggacaat tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc
     5821 gatccgttcc attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg
     5881 gtgtaggtaa ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt
     5941 atttaacaaa gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac
     6001 ggatgaattg aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt
     6061 tttagagctt gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga
     6121 aacagtcggt ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc
     6181 aaagaaggat aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac
     6241 gta
//
LOCUS       DACESX010000141         6210 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.142, whole genome shotgun sequence.
ACCESSION   DACESX010000141 DACESX010000000
VERSION     DACESX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.142"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(99..1229)
                     /locus_tag="IXF88_002385"
     CDS             complement(99..1229)
                     /locus_tag="IXF88_002385"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1509094.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IXF88_002386"
     CDS             complement(1878..3080)
                     /locus_tag="IXF88_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1509095.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IXF88_002387"
     CDS             complement(3140..3343)
                     /locus_tag="IXF88_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1509096.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IXF88_002388"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IXF88_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IXF88_002389"
     CDS             4880..5692
                     /locus_tag="IXF88_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1509097.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1838 a         1281 c          990 g         2101 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga
//
LOCUS       DACESX010000142         6173 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.143, whole genome shotgun sequence.
ACCESSION   DACESX010000142 DACESX010000000
VERSION     DACESX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.143"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            725..811
                     /locus_tag="IXF88_002390"
     CDS             725..811
                     /locus_tag="IXF88_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1509098.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IXF88_002391"
     CDS             complement(932..1621)
                     /locus_tag="IXF88_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509099.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IXF88_002392"
     CDS             complement(1628..1906)
                     /locus_tag="IXF88_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509100.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IXF88_002393"
     CDS             complement(1866..2780)
                     /locus_tag="IXF88_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1509101.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IXF88_002394"
     CDS             complement(2762..3142)
                     /locus_tag="IXF88_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1509102.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IXF88_002395"
     CDS             complement(3592..3879)
                     /locus_tag="IXF88_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1509103.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IXF88_002396"
     CDS             complement(3899..4123)
                     /locus_tag="IXF88_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ1509104.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IXF88_002397"
     CDS             complement(4763..5296)
                     /locus_tag="IXF88_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1509105.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IXF88_002398"
     CDS             complement(5317..6159)
                     /locus_tag="IXF88_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ1509106.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACESX010000143         6091 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.144, whole genome shotgun sequence.
ACCESSION   DACESX010000143 DACESX010000000
VERSION     DACESX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.144"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(33..881)
                     /locus_tag="IXF88_002399"
     CDS             complement(33..881)
                     /locus_tag="IXF88_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1509107.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            complement(1038..2648)
                     /locus_tag="IXF88_002400"
     CDS             complement(1038..2648)
                     /locus_tag="IXF88_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1509108.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            complement(2892..3209)
                     /locus_tag="IXF88_002401"
     CDS             complement(2892..3209)
                     /locus_tag="IXF88_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1509109.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            complement(3202..3981)
                     /locus_tag="IXF88_002402"
     CDS             complement(3202..3981)
                     /locus_tag="IXF88_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1509110.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            complement(4114..5049)
                     /locus_tag="IXF88_002403"
     CDS             complement(4114..5049)
                     /locus_tag="IXF88_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1509111.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            complement(5125..5517)
                     /locus_tag="IXF88_002404"
     CDS             complement(5125..5517)
                     /locus_tag="IXF88_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1509112.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            complement(5498..6016)
                     /locus_tag="IXF88_002405"
     CDS             complement(5498..6016)
                     /locus_tag="IXF88_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509113.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
BASE COUNT         1969 a         1269 c         1034 g         1819 t
ORIGIN      
        1 atatttttgg tatgtttttt gaataaaagg aattattgcg ctgaaatggc aatgccgatg
       61 gctttttcac ctaaatgtgt accaatgact ggtccgaaat gaccaatttc gatttctgca
      121 tttggatatt ttttctgcaa tttctctttc tccgcaatcg ctacttctaa attatttgca
      181 tgtatcacgt aaagtttgac tggcggttgt atttcgttat ttcttctacc aataatttct
      241 tcagctcgag caaatgcttt tttacttgaa cggatttttt cgaataaaac aattttccct
      301 tcagaaaatg tcaggatcgg tttgattttt aataagctgc caatcagcgc tgcaccattt
      361 gtcaaacgtc ctcctcgtac taaattgttc aaatcatcta caatcagata agcataggta
      421 ttatctcgga ttcgatcgat atgtgcaagt atttcatcaa gggactgacc ttcatcattt
      481 aagtctaagg cagcttctac catgtgtccc atcggcatac tagtgatctt agagtcataa
      541 ggaatgaccg ttactccgct gatgtcatct ttcatcgcaa ataatgtgtt gacaaagcca
      601 gaaatccctg aagaaagatg gatacttaaa atcgtgtcat accctttctc tttcaattcc
      661 tcatataaac ccaaaacttc ccctacgaca ggttgtgaag tagtaggaaa atctttactg
      721 ttttttaaaa gtccataata ttcatccgct tcgatatcga ttccttcgtt ataaattttc
      781 ccatccataa tcaaagggat tggaataacg aataagtcgg gatggttctt gatacgttcc
      841 ggcagatatg ccgaactatc tgtaactact gcaagtttca tgtgtttaat ttcctcgttt
      901 caaagaattt ttccagacct tataactata tcataagtaa gattagatat gaaactaaaa
      961 ttggaaaata gaaaaacgac aagcgtaatc atacgagttg ccgtttttca agtgacagca
     1021 aaaaacgatt agttaaccta ttctttgctt ctgctatgat gtttaacatt tttcaacatt
     1081 cgaaacccat tttttccgaa ttgagaaaat aaaaatacta agccgccaat acttcctaca
     1141 gccacacaat aaatcattgc atagcttttt gattccattg acaatcgctg attcagcaaa
     1201 aagaaaacgg caaaacatac tgcaagcata atcagggtgg agcggaaagt ttttaagaaa
     1261 aaattgctga tctttgctaa aggtgctatt cggtattctt tgcacataaa gtgatagccg
     1321 cgaacaaaga caataccgaa tgctaagcca tttgataagc tcatcccgta tccgccaact
     1381 aatcctaatc cgattacttg gaagatgact ttcaggataa ttgcttctac agttaatcgg
     1441 atcgcaaaac gatggtgatt caaagattga agcatcgtag ataggatcgt gaataaagag
     1501 aaaaacaaag aagctagcaa agccatttgg aaatatccga cactttcagg atcatagcca
     1561 aaaaacaatg tgtatagtgg tccagctaat aaagacattc cagtcaatga aggaagaaga
     1621 atcaataacg caatcgaaaa actatctcct actgtttttt cgatttgtat ccgatctttt
     1681 ttcttcatcg tgcttaatat tggcaagctg cttatagcta ctgttcctac catggagatc
     1741 aggatcaatg tcaatttatt tggattgaca gaagcacggc tgaataagaa ttccagttgc
     1801 tgatctgcga tctctggacg gaaaaagtga agcagtggtt tcatcgttac ttggtcgatc
     1861 atttgaacga tagtaatgac tgagccgaca aaaataaacg gtagtgtttc ccgaatgata
     1921 gagacagagg cagatcggtt ttctctttcg aaataacggc tggaaatcaa aaaatcttta
     1981 agttcgaaga agtcttttct gcgtccgacg atatacatat ggataatcgc agctaaccca
     2041 ccgaagaaag aagcgactgt actgatcaat acagcttcta atatggttcc gtcagtcaat
     2101 acgcgcaagt aataagtacc agccaagata acgatcactc gcacagcctg ttcgataatc
     2161 aaagaagttc cataaggccg cagatcattt tttccttgga aatatcctct cattgcacta
     2221 agaattggga tagcaatcaa agatgggcat aaacttctga tagctagtat cccattatta
     2281 acgttagcaa tcggactgat tccggctagt aaaggagcaa ataaatacat cagtgcagca
     2341 gaggcgatac cgatgacaaa catgatattg atcgtacttc taaaaatcag ccgacaagca
     2401 gcgttatctc cttcttttgt ggctacagct actttttttg caatcgcatt tggaaatccg
     2461 actgtaccaa gcatcaggaa caatccataa ggaagatagc caacattata caatgtcgta
     2521 gcaagcatac catcttgatt gttccccatc atacttagcc agggaatcaa atagactacc
     2581 cctaaaactt tacatagaag attagcaaag gttagccaaa atgttccttg catcaatttt
     2641 ttattcattt aaattccctc aactattctt tcataaaata aacatcttcc ctattatagc
     2701 aaaaaatcat agaaggacaa atccctctct aattcctaaa ataaaaaaga gcttgcatca
     2761 taagccaaca ctaatctttt ttatccctac gaataaactt gcatccagaa ttgactgttg
     2821 caaatgcagt tcggatagat cagtttgaac aacttatgtc gcaatctctg tttttaataa
     2881 aataaaacag tttaaagcat agacagcaaa aagacagacg caagtcctgt caaaactgac
     2941 aacgccatcg aacgtgtttt gtaagccacg cctgctacta gaatcattgc agctattttg
     3001 atattccaag caatatccag atgttcatga gtaatgaaaa catctttaaa agctaacgcc
     3061 gcaaataatg ccaccggtac atatttcatc cagtcactga accaggatgg cacttttcga
     3121 tgagtaaaat aaagcatcgg gaacaagcgc gggatatatg ccacgatgaa cagacaagca
     3181 actaaaagta gcaaatatag tttagtcatg gtgattggcc tcctgttccg ggaagttgaa
     3241 gagcggctca ttgacatctt taccgcggtc tcttagtttc ttagcttttt cttttttgaa
     3301 tgccttctcc agcataaatc cggcaaatga agcaatgact gttgctgcaa tcaatccgaa
     3361 agtattttgg aataatacca tgaatacgac ccctaatacg cctgagaata aacctgtaaa
     3421 gatcagtagt gcgcttttca tctgcatgat aaacatgaaa atgaacatgg ccgtcaaagc
     3481 aaaatggaca aggtcagcat ctactcgaat caccgagccg aaaaaattac cgatcatatt
     3541 gctaccggcc catgcagtca ttgagaacca attgacatat aaagcctttc ttttattcca
     3601 ggaagggtca gttgaaaact ttaagtagtt caccgcatag ttttcatcat tcagtgattg
     3661 agaaaagaca gccaaaaacg tccgcttttc ttttttcata aaaccggcaa gacttgctcc
     3721 taataatgta tagcgtaatt ctaaaaagaa aaccatcaat aatgtcgttg caaatgatgc
     3781 ttgcgtcgta agcatagatg caactaaaaa ctgcgcgcca ccggaaaaga ccagaatcga
     3841 taacagtcct gataagagag gatcaaatcc aactttttgc aacaatactc cgcaagctaa
     3901 tcctactggt atatagctta tacaaagtgg catcactgca tgcaaagctt gaagccatgg
     3961 cgcacttttc tttttgttca ttaaaatatc tcctttaatt acaacaaata aacagcggac
     4021 tcattgtaac ataaattcgc taaattgtag ccgttttatt taaaaacaag attatatttt
     4081 tctcattttt gcttcaattc atgaataaac gttttattta cgagaataat ttgggtcata
     4141 tttatctgtt tcaatgtata tatacatctt cttgtcagga agagactgac gcatattttt
     4201 ttcaatatca ttgatgaccg agtaagcttc attctctttg ttaccaacga tatctgcttt
     4261 tgctgcaact aaaatctccg ttggacttaa atgaactgtt tttatatcga tcagccgact
     4321 gatctcagga cgttcaaaag ctaacttgat ttttgataaa tcgcttgctg tcacgctttc
     4381 accgatgatc agactataaa attcttttgc taggaaaatg gcggctccca tcagcaagaa
     4441 accgatcagc aatccgctga tcgcatcaaa tgcagcaatc ccggttgcat aagtcaatat
     4501 tgttcctgct aaagccaaga ccaaaccgat tacagcacac aagtcttccg taaaaataat
     4561 cagtatttca ctgtgtcgac tttcgcgcaa aaagcgcatc gtggaaagtt tttcagtatt
     4621 caattctttg atttctttca ttgccacgcg taacgaactt ccttcgatta ccaaaccaaa
     4681 taacagtatt gctataacaa tgatcggatt acccacttca tgggatggat gcgtcaactt
     4741 ttcaaaagct tccatcaccc caagtgcccc accgccgaag aaaagcatca ttgcgacgat
     4801 tgtactgaaa aagtattttg ctcgtccttc accaaattga tgcaactcgc tctgtccttt
     4861 attggcttgt ttatcaccga ataacagcaa aatctgattg ctgcaatcaa cgatactgtg
     4921 gatgctttcg tttagcatgg cggcactgcc actgattgca tagccgacaa atttgctgat
     4981 agcaactaga atattagccc ctagagcagc aattacggaa aacattccgc tttttttaaa
     5041 attttccatg ttattttcct tctttcatca actgaactca ctgaattatg gcaatattcg
     5101 ccctttaaca gttttttatg aaacttatag tttagacaaa ttgaaaatga ccgattgtcc
     5161 atcattcaat ttgatctctt tggcaccagt agtagcactt ttcccgtcca cagtaaacat
     5221 ccaatagagt tgatttttct catcctgtga gtgcccttca agcgaagtga tgaagccgtt
     5281 atcttctttg acttcaaaat ttcgtttcaa aatactcagt aaatcatcgc cttttttgaa
     5341 gaccacttct ttttccgaaa agttttgacc attttcttgt aacgtgattg ttgctttttg
     5401 cgatgttttt acttcctcct tttggttgga agccgattgt gtataggttg tactgcaact
     5461 tgtcaaaatg agtatactga cactcattgc aagcaaacta taaattattt ttttcatttt
     5521 ttcctcctga atgattgtaa aaacgtttga cgactggcag aaaaacaaga aagaacacaa
     5581 cattgcctat actgtgcatc aaatcaaacg acaccccttg aaggtaatag atccaaggtt
     5641 gaggcaaacg atataacagc gtatcaaaaa cggtcatacc tattccgtaa accatgcccg
     5701 ccagaaaaaa acagactccc tttaaccata gattcctttg cactgttttt ctcaagcaga
     5761 cgattgaaaa caaactcaga acaacagtat agctgatcca ttggccgatg acccatggtc
     5821 caacaccaaa ataaaaactg ctgatcgcaa tagaaagact catgacaatt aacgcatctt
     5881 ttaagcttaa gtaaagtact ataaataaaa aaatagcagt cattggctgg aaattcggaa
     5941 taaattgaaa agcgatccta cctgctacac aagcagcaga caataagcta agctgtgcta
     6001 ttttatttgt ggacattcct aatgaaatcc ctccttcatc agcaagttat tcacgtccat
     6061 ttagtacata cagaaaagac ttgtgacaat t
//
LOCUS       DACESX010000144         6002 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.145, whole genome shotgun sequence.
ACCESSION   DACESX010000144 DACESX010000000
VERSION     DACESX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.145"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            240..2471
                     /gene="pflB"
                     /locus_tag="IXF88_002406"
     CDS             240..2471
                     /gene="pflB"
                     /locus_tag="IXF88_002406"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1509114.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2715..3476
                     /gene="pflA"
                     /locus_tag="IXF88_002407"
     CDS             2715..3476
                     /gene="pflA"
                     /locus_tag="IXF88_002407"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1509115.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDIFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3561..4487
                     /locus_tag="IXF88_002408"
     CDS             3561..4487
                     /locus_tag="IXF88_002408"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1509116.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4669..5562
                     /locus_tag="IXF88_002409"
     CDS             4669..5562
                     /locus_tag="IXF88_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1509117.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5696..5821
                     /locus_tag="IXF88_002410"
     CDS             5696..5821
                     /locus_tag="IXF88_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1509118.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
BASE COUNT         2125 a         1014 c         1184 g         1679 t
ORIGIN      
        1 taatacaggt ggcgtctctt tttcattcat ttttaataaa tccttatacg tttgcaaaaa
       61 aagatgaaag tgttcacaat gcttaaatgt tggtatgtca acgttgattt aaatatatgt
      121 aaataaaaca taaaaattgt taaataaaaa acaagaaaac ccttgcaaag atagggtgct
      181 gtgttatact aatcacgtaa aaactgttac ataaaacaga aatttaggag gtccatacaa
      241 tggagcaatg gaatggattt aaaggtacca aatggaaaga aggcgtagac actagagact
      301 ttattcaaaa taactacact gaatatcgcg gtgatgacag ctttttggaa ggcccaacag
      361 aagcaacaga aaaattatgg gatgaattgc aaaagttaaa tgatatccaa ttcgagcgta
      421 acggtgtcta tgatatggac acagaaatcg tatcaactat tacttcacac gatgcaggct
      481 atttggataa agatttggaa aaaatcgtcg gtctacagac agacaaacct ttgaaacaag
      541 cattcatgcc gttcggcgga atcaatatgg ctaacaatgc gctaaaaagc aatggctata
      601 caccaaacga tgaattgaca catattttca ctgactggcg caaaacacat aatcaaggtg
      661 tatttgacgc ttatactgct gaaatgcgtg ctgctcgtaa aaacaaaatc attacagggc
      721 ttccagacgc ttacggtcgc ggacgtatca tcggtgacta tcgtcgtgtg gcattgtatg
      781 gaatcgacta tttgatggaa caaaagaaaa aagatcatga tatgactggt tatcatacaa
      841 tgagtgaaga agtgattcgt ttacgtgaag aaatcactga acaatatcgt gcattagcgc
      901 aaatgaaaga aatggctgcg aaatatggct atgatatttc taaaccagct gcaaatgcaa
      961 aagaagctgt ccaatggcta tacttcggat atttagcagc aatcaaatca caaaatggtg
     1021 ctgcgatgtc aatcggacgt atctcagcat tccttgatat ctatatccaa cgtgatttgg
     1081 ataatggagt aatcacagaa aaagaagcac aagaattgat cgaccactta gtaatgaaat
     1141 tacgtatggt caaattcgcg cgtacacctg aatataacca attattctca ggattcccta
     1201 tctgggctac tttatccatt gcaggtatgg gattagatgg tcgttcatta gttacaaaga
     1261 atgatttccg tctgcttcat acattaacaa atatgggacc ttcaccagaa ccaaacttga
     1321 ctgttttgta ttctgaacac cttccagaag gattcagaac atatgctagc agaatcgcta
     1381 tcgaaagttc ttcgattcaa ttcgaaaatg ataatcagct tcgtgaacaa tggggttctg
     1441 atgattgtgc gattgcttgc tgtgtatcag gaacagttgt cggaaaagac atgcaattct
     1501 ttggcgctcg tgctaacttg gctaaaacac tattatatgc catcaatggc ggagtcgatg
     1561 aagtgactgg cgcccaagtt ggaccagaat atcgtccaat cacatcagaa atactagact
     1621 ttgaagaagt gaaacataac ttcatgaata tgatggattg gttagcagaa ctatatgtca
     1681 atacgttgaa catcattcat tatatgcatg ataaatattc ttatgaagca gctcagctgg
     1741 ctttgatgga ttctgacttg caacgcacat ttgctacagg tattgctggg atctctcatg
     1801 cggcagacag cttgtcagcg atcaaatatg caaaagtaaa agcaatccgt aatgaaaaag
     1861 gaatagcagt tgattttgaa gtggatggcg acttccctaa atacggaaat gatgatgacc
     1921 gtgcagatga attagtttca tggatcttgg aatacttcat gggacaaatc aaacgtcata
     1981 aaacttaccg caactcaaaa ccaacaactt cattattgac gattacttca aatgttgttt
     2041 acggtaaaaa tacaggaaat actcctgatg gccgtgaagc tggaaaacca cttgcgccag
     2101 gagcaaatcc aagttacaat gcggaacaaa gtgggttatt agctagcctg aactcaacag
     2161 ctaaaatgaa ttacggctgt gcattggatg gaatttcaaa tacccaaaca atcaatcctt
     2221 caggtcttgg aaaagaacaa gatacaagaa tcgaaaactt gcgtaatgtc ttagatggat
     2281 acttctcaaa aggagcttat catttgaatg tcaatgtatt cagtaatgat ttattgcgtg
     2341 atgcaatgga aaatccaatg aaatatccga acttgacgat ccgggtatct ggttatgcag
     2401 ttaaattccg tgacttgaca agagaacaac aattagatgt cttgatgaga acttcacacg
     2461 acagaatgta aaaagttcgt gaaaaaagtt tttcgactta gcgtcgaaag gacgttgaat
     2521 agccatcgca tcaaaatcga ataatcagta tgcttatttg aaagaaacga tttacagggg
     2581 caggacttgt atgttccgtc cctgtaaatt ttacacataa cgaagcggca attgcgaaaa
     2641 attttgttca gaaagcttcg cttcgacagc taacttgtac ttaaaatgaa atcaaagaaa
     2701 agatgtgaac ttaaatggaa gaaaaaacaa tcggctatgt tcattctatc gaaactttcg
     2761 gttcagtaga cggacctgga ctgcgctttg tcgtgtttat gcaaggatgc cgcatgcgtt
     2821 gccaattttg tcacaatcca gatacttgga atattggcgg agggaaagaa tacacagcag
     2881 atgaactttt agataaagca gaacgcttca gaccttactg gggagataaa ggtggtatta
     2941 ctgttagtgg tggagaacct ctattgcaga tcgatttttt gatcgaacta tttaaaaaag
     3001 caaaagagcg gaagatgcat actacattgg atacatgcgg aaaacctttt acgtatgaag
     3061 aacctttctt ttcacgtttt caagaattga tgaagtatac agatttactg ttatttgaca
     3121 tcaaacacat cgataatgaa gagcataaaa aactaacgca ttgggacaat gaaaatattt
     3181 tagaaatggc tcaatatttg tcaaaaatca acaaaccggt ttggattcgc catgtactag
     3241 ttcctgagcg aagtgattat gatgaatatt tgatccgact agacatcttt attcaaacct
     3301 tgtcaaatgt agacagagta gagattcttc cttatcatac gatgggaaaa tacaagtggg
     3361 aaacacttgg tttgaaatat ccattagaag ggatcgaacc acctacaaaa gaacgtgtcg
     3421 aaaatgctaa acgattgctt catacagaag agtatacaaa atatttaacg cgataggtcg
     3481 catcggaaag aagataagta ttgcttcttt ccgttttttt gctattatta aataggagaa
     3541 atcacaaagg agcgaatgac atgtcaaaaa ttttagtttt tggtcaccaa aatccagata
     3601 cagatgcaat cggggcagcc attgcgtttt cacacttgca aaatgaatta ggtaaagaaa
     3661 cagaagcagt tgctctaggt acacctaatg aagaaacaca atacgcatta gaccactttc
     3721 agctatctgc accacgagta gtagagtcag ctaaagcaga aaatgcagat gtcatgcttg
     3781 tggatcataa tgaattccaa caaagtattt cagatatcag cgaagtaaat attttagctg
     3841 tagtagacca ccatcgtatt gcaaactttg aaacagctaa tccattgtac tatcgtgcag
     3901 aaccagttgg atgtacaagt acgattattt taaaactata taaagaaaac caagtaacaa
     3961 ttcctaaaga aatcgcagga atcatgctct cagctatcat ttcagataca ttgttgttca
     4021 aatcaccaac atgtacgcct gaagatgaag cagctgcaaa agaacttgca gcaatcgctg
     4081 aagtcgatct aaacacttat ggacttgaaa tgttgaaagc aggaacaaat ctaagtgata
     4141 aatcagctgc ggttcttctt gatttggatg caaaaagttt cccaatgggc agttccaacg
     4201 ttcgtatcgc tcaagtcaat acagtggacc ttaatgaagt gatggatcga caagcagagc
     4261 tagaagcagc aatggaagat gaaatgaaag caaatggcta cgatctgttc gtattagttg
     4321 tcacaaacat cttagacagt gattcagaat tacttgttgc tggtaaagga aaagaaaaag
     4381 tagaagaagc tttccaaaca actttgacca acaaccgtgc tttgcttaaa ggtgtcgttt
     4441 cacgtaaaaa acaagtcgta cctcaattga cggctgtatt tagctaatca aatggtagta
     4501 tgaagcattc aaaatgaatg gataagcgat tgatataaaa agaaacatag aacatactaa
     4561 aggccgaaga aatggaggag aataacagtc aattatgact cttgttcttc ctccttttta
     4621 cgtaaaagcc ttttaagtaa aataaaacgg atagaaaaga gaacatatat gaactattat
     4681 tcaaaaaata aggaaaactt ttatcaaaaa ctgaccgatg atccgctttt ttcttcattg
     4741 accgattatc tatatgaaca tcgagaacaa gaaacgattc taagagaatt gaaaaaagaa
     4801 ttttcgcaaa ataaattttc ccattttctt gatttattga tcgatgcagg attaatcaag
     4861 agggaagaac gaagatatca tttgaacttt ccaatatttg atcctaaaga ttacttgcaa
     4921 caagccactt cggctgctga aaccatagct gaacaattga agagactctc tgttgatgaa
     4981 caaaaattag cgatgggaga agttatttgg gcgtattgtt tcgaagatga acggaaagaa
     5041 gcgtattttt atggagtaag aaacagccga gagacagaat tgctgaggac aactgcagga
     5101 aatgagaagt accgttttat cacactttcc tctatagaac acttcccgct tactttggct
     5161 aattactttt ttgtacagaa gaatcagctt cctgtaacga aggcattcaa agaattagcc
     5221 gaacttattg gagacgtaaa tgaatcgtac ttttttgatc aaatagaagt tatcgttgac
     5281 aggatacgta agaataaata taaaaatcgc cggcctagta ttttccatca gtctctactt
     5341 gtgacgaata ctataaagga agaagaatca ttcacattag aattacctat tgtcgaaaaa
     5401 aacaattttg agattgaatt accaacattg gatccttcat tgacaatgga ggaaatggcc
     5461 tttcttaaac gacaaatctt ttctgagctg agcaagaaat ttataccgca tgcattttct
     5521 tatatcaaag aatatggaac agttttagtg agtaaaacct aagagagcag taagcaaaaa
     5581 ttatactgac tcttgcagga ggagcctttc tcttccttta tggttaaaag aattatttga
     5641 gtattttgcc tatctaccac ctattagatt cactacatgg tctggaaaca aaggaatgca
     5701 aaaaaatggt gagaaatgtg gaatgacaaa ggaagtagtg ataagaaaag ttagattttt
     5761 aaataatcaa tattatgatt cagtcaagta tggcatttta tgggaagaac tagctgatta
     5821 gattaggaag tccatttgat aatccagaat cactaagaaa cgaatggcga gtcattaata
     5881 atttatccaa tcttgggtta ttcttttaga ttgaagtgtt taaaactaaa aaagcattta
     5941 tgaagtcgta atagtcaggc ttcaaaatgc tttttatttt gtttgttttt tctttgccac
     6001 at
//
LOCUS       DACESX010000145         5911 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.146, whole genome shotgun sequence.
ACCESSION   DACESX010000145 DACESX010000000
VERSION     DACESX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.146"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            96..3374
                     /locus_tag="IXF88_002411"
     CDS             96..3374
                     /locus_tag="IXF88_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1509119.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3915..5822)
                     /locus_tag="IXF88_002412"
     CDS             complement(3915..5822)
                     /locus_tag="IXF88_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1509120.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
BASE COUNT         1872 a         1121 c         1221 g         1697 t
ORIGIN      
        1 tacacttatt ataattttgt tttttattga cagtaaaaat aaattaggta atactttaag
       61 taaacgcttt ctaatttgtt acgggaggaa tacacttgtt aaaaaaacgt ataaaataca
      121 gtgcgctaat gatgaatctc cttatgctca gtaccccggt tttaatttct gtcgttgaag
      181 ttgctgctaa cgaacaacag tctgtgaacg aagaaaatgc acacgtgaac attgaaggga
      241 tgtcaacaat ggaggaaata ccaagaacta gtgaagatat ggatacggaa gaaggaaatg
      301 atcctatgat gaaagaatca gaaaatgaaa agacaaaaaa gcaatctgaa gaagcaaatg
      361 aagagtggaa aggggttgtt ttcggagaaa gcacaagtgc tagtaggtat gccattgaac
      421 cattagaaga cggcgtgcgg ctctcttcca cgaataacgg cggaaaattc cagtcaagcg
      481 gatcagatgg aatgacctat tattatcaag caatccccaa agacatcaac tttaggatgc
      541 gggcgacgat cgaaatagaa tcttggacct atacgaatgc ccaagaagga tttgctttaa
      601 tggtaagaga cgcagtacca aaaaatcatt tgtttgggca ggcattttat tcaaattcat
      661 ttgccatact aggcagtcgg atcgaatatg tctgggactc ggatagacaa gaagtggtca
      721 acacgagcgg cagcaaatat acgatgcgac ttggattggg gacacgggca atcacaggaa
      781 tatcatctga agatccgcaa gctgcgccag ctcctgggag tgtctcagtg gaaacaacac
      841 ctttagagac ttcagcaggg ttcttggaaa aagaaacagg ttcttataat atattcggaa
      901 acggtcatgg gaatctgttt ccggccacga actcgattac taaactggac gtagagatga
      961 aaaaaacgaa tactggttta gaggcatatt attacgatcc tgaaacagga gaagaaatgg
     1021 cttctgacat catgtatgac tgggaaaaat tatacgcttc agatgaatcg gttatttatg
     1081 caggatttgc tgcagcaagg aatatgacga tcaaggtaga aggaatcgaa gttgcttact
     1141 cagatcctgc gacagatcct cctgggcaag aaagacctac tcgcttgatt gatccgattt
     1201 acactgtgac ctctagtaat cattcaggaa accaagatca tactatgtcc tttagagcaa
     1261 atgctgacgg attattgaca attaaaaata aagcaacagg agaggtgctt aagaacgcaa
     1321 aaaacttggc tatcactgcg aatcgggaat ttgattatca gacaaaactg atggaaggaa
     1381 cgaacgaatt tactttcagt tttacgccag ataaaaacta tccgccagaa gaatacgtag
     1441 agatgactag ttatgaaaca aaagagattc ttcatacagt ggactaccgt aaacctaaat
     1501 attcgacagt ttatgtaaca ccagaagggt cagatgcagg aaacggaagc agacaaaatc
     1561 cattatctct gacacaagca ttgagatacg catatgctgg gcagacaatc gtcatggcgc
     1621 ctggaactta ttccttcgaa agaggattga cgattccaaa aggcgttaac gggacaagag
     1681 aaaacccgat tcagttgatt gcagagaaaa atccagaaaa caagcgtcct gtacttgatt
     1741 ttaaaggaac tggaaatggg atgacactgt tcagtcacta ctggggttta cgagggtttg
     1801 acattacaaa tagtgcagct atgcaaaaag gattacagat ttctggaaat cataatttga
     1861 tcgaagagat tcatgcttat cggaatggaa atacaggaat ccaaatcagc ggtagctcaa
     1921 atgatccttt cgaagcatgg cccgcgcata acttagtcaa gaactgtaca tcttttgaaa
     1981 atgccgatcc aggatttgaa gatgccgatg gatttgcggc caaactgaca ataggagaag
     2041 gaaatgtatt tgacggttgt atcgcgtatc ataatgcaga tgacggttgg gacctttttg
     2101 ctaaaaatga aacaggttcg atcggaaaag taatcatcaa aaactcagtt gcttatcgaa
     2161 acggctgggt accaggaatt gagggagaag gtaacggtaa tggattcaaa atgggcggtt
     2221 cttctttaac gggccctcat gaattgatca atagtttgtc tttcgaaaat cttgcgaaag
     2281 gaatcgattc caatagcgga acagatatta tcgtcaatag cagtacaagt tttaataacg
     2341 gttcatataa tgtggctctt tatacctctt cagcaggaaa cacagactac catgcttctg
     2401 gtatcttatc tttccgaaca gagaatcttg agatgagaga gcagatccgc ccattgaatc
     2461 aaaacgaaga tcaaatctac cactcgttga actactattg gaatgaacga gagaaacaat
     2521 cagaaaatac tcttggacat accatatcaa aagattggat cgagtctcta aatacatgtt
     2581 cgaaagaagg tcagcagcct gttacgcgct atgaaaatgg aagtatccat gttcatggac
     2641 tgatgcagat taaaccgggg aatcgtcaaa cagaaaagga aagagtggat ggtcttccat
     2701 tatctgtagg tgctattttt gacggagaaa cctcaccgtc ttcggaatat ccagagtatg
     2761 caatcgtaca agaaccaaat cagacaaaac caaatgaaca aaataatccg attattatac
     2821 aagaagcaaa tggtgtggcg gagaatgcag aaggaaatat cattgaactg aagcctttta
     2881 cagtttttgt gtcaactttc acaccaaatc aaggaacgcc tgtctttgaa gatatgcgtt
     2941 atttcccaga aaagatccaa atcgttgaaa cacagtcaag tgctcaattt tctaatcata
     3001 ccgctatttt ttcagcacct tcaaaaggag aatatacgct agaagtcact tatcgttcgg
     3061 aaatatttga tggggaaaaa tggtctgctc atacagaatc tgttacccag caaaaagcga
     3121 tcactgtgcg agaaactgcc gatgtgcccc cgggaaatga caaaccagaa agagaaaatc
     3181 caggaaatga atcaccaaat ggaaaaccaa atggaaaacc ttctcatccg gataggacag
     3241 caaaagaaag tgaaaaaaat ggcactcaag gactcccaaa aacgggagaa tttaataacc
     3301 cgttgctagc attagctgga ggaattttgt taatcggtgt agttgtttat gtcatgaaac
     3361 agcgaaaaaa ataagtggaa tttctccttt tatgatgtaa aacaattgac tgaggaaagt
     3421 aaaaatagaa ggaaatgaga ccaatagatg tctcacttcc ttctatcttt ttatagatga
     3481 ggttctcctt gctgcttttg attggctgtt cttattactt gatgcaggac agctttttca
     3541 caatctctcc actaaacggt gttcaatcag ctgaccctcg gtattagctc aacaaacggc
     3601 aaaatatgag gaactatttt tccgtttttt tctctaatac tcaggtcagg acgctgattt
     3661 cacaacctct cgataaacgg tgttcaaaag ccagtttctc ggtagtaagt caaatttatg
     3721 caaaaatgtg gaaagccatt ttcacaaaat ttttcttact acttgaaact aagcagcttt
     3781 ttcacaacct ctaatacatg gtattccaga agtaacttct tcgaaaaata agccgaaatg
     3841 tcacaaaaat ttgagaagcg attttcccaa attccttctt atttcttgaa gctgaccact
     3901 tctggcacaa cctcttacca gattcgcact cttttttctg gcgctagata catagcgtct
     3961 tgttccgtga taccaaatgt ttcatagaat tcgtcaaggt tttttggttg cacgtttgct
     4021 cggagtttag caggggcatg gacatcgatt tgcagtaaaa gttgttggta ttcttttttt
     4081 gcttttgtac gccaaatcgt tgcccaattg acaaagaatt cctcgataga aagatctgat
     4141 tccgtttttg ctgcttccaa tgcacagctt agaccgcctg catcagcaat attttccgaa
     4201 acggttaatt ttccgtttac ttttccgtca gcaaaaggca gaccatcgaa ttcttcgatc
     4261 attgcttgtg ctttgttttg aaattgtgta agatcttctt ctgtccacca gttgttcaag
     4321 ttgccatatt cgtcaaataa agcgccgtta ttatcaaatg catgggaaat ctcatgagcg
     4381 ataacagctc cgattccacc ataattagca gagcttgatt gttctagact gtaaaatggt
     4441 gcttgaagga tcgctgccgg aaagacgatc acattccgaa atggatgata ataagcattg
     4501 accgtgtcag cactcatttc ccattctgaa cgatccactg gtttgttcca tttgctgaaa
     4561 cgatctttta atgtgagttt gctgaaaaca agagtatttg aaagcaaagt accaccctga
     4621 tctgcaggta ctgttttgaa ctggtcgaat aatgctggga tcttatcggg gtagcctact
     4681 tggataccta agttatttaa ttttatgacg gctttttctt ttgttgcatc cccaagccag
     4741 tcattttctt ctagacgttt tttataaacg gcgatcattt ttttcaccat ttgttctacg
     4801 tcttttttag cagcttcgcc aaaatatttt tttccgtagt aatcgccaac tacttgatcg
     4861 aattgtccag aggcaaggta atacgctgct tttttctgag gcattgcttc gtctgttcca
     4921 gacaatacgc gagaatagat tccgctgact tggcggaatt cttctgatag ataaccacta
     4981 agagaacgta ctgttttaat gatcatccag cttttgaata acggaaaatg agcttctgtc
     5041 aggatgtcat tcaaatgatc gaagtacact ggatcagtta cgatcacttt atctggagtt
     5101 gtcccaatca aacccgtgac aagttttttc aagtccagtt tatctgtgta tttaataaat
     5161 gtatcaaaag attgcggatt atacattttg ctgtaatctg cgttttcttc tgcacttttt
     5221 acatgaggag caatcaattt atcaaattgc actgcttgtt ccgtgattgc ttctgcctga
     5281 cttttttctt tgccggcaag ctctaataac tgaacagcca tatcaaagaa aacgcctatt
     5341 agttgtgcac cgttgggatg gtctgatgca taatacgtct tatctggaag gaataaagaa
     5401 ggcggataag caaagaaggc gttcgtttgc gcattcttca tatctgcatc tacatccaga
     5461 ttaaatggca atggtaatgt atccaacagc cattctggaa attgcttatt caattcgtcg
     5521 aaagactgca atgattcaac tttttcgatt ataggttgaa gcggttgagc atgcaactca
     5581 tttcttcggt cataatcgtt tgccagttga taatatgcaa ggaaatgttt cattttttct
     5641 gaagacactt tttctggatg ggcaagcaga tcgtctgttt ctcccatcaa aagttggtcg
     5701 atcccgtcaa ctaaatcttg gaaaccgcct gtagctggtt tatctgcagg gatttttgct
     5761 gttttttgcc attcttcatt gactgcttca tagaaatctt gtttcaatcg ttctttgttc
     5821 attcatcgat cgctcctttt ttgtttcgtg tgcccattat cttacaaaat gaaggaatta
     5881 acaaatagaa cataaaaaat tgaaaattcg a
//
LOCUS       DACESX010000146         5840 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.147, whole genome shotgun sequence.
ACCESSION   DACESX010000146 DACESX010000000
VERSION     DACESX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.147"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(305..1087)
                     /locus_tag="IXF88_002413"
     CDS             complement(305..1087)
                     /locus_tag="IXF88_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509121.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1152..1988)
                     /locus_tag="IXF88_002414"
     CDS             complement(1152..1988)
                     /locus_tag="IXF88_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1509122.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2006..3415)
                     /locus_tag="IXF88_002415"
     CDS             complement(2006..3415)
                     /locus_tag="IXF88_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1509123.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3429..5252)
                     /locus_tag="IXF88_002416"
     CDS             complement(3429..5252)
                     /locus_tag="IXF88_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1509124.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1798 a         1264 c          952 g         1826 t
ORIGIN      
        1 cagatacata tgaaacaatt acatatgatt ttttcataaa aaaacagtac tgtctagtag
       61 tataaaatat acaaatttct taaaaatttt ttcactaaaa tcactacctg aaacagtcta
      121 aaacacttat atatcaacgt ttaatgaaac tatctccgtt tcatttcaga tacaaataaa
      181 tatagtcggg ctccaaatcc gaaaaaaaat acccccagta gcttatccta agctactgga
      241 gatgtataag tacggtcata taacaacggg gtagggtaaa taaatgaaaa agatggaaga
      301 atcattattc aataactttt ttaaatactt cgttgtaatc agttacctgt ccgttcttaa
      361 ataccacaga ataatctttt ttcccatttt caagtaaccg aagtggaatg tctttaccaa
      421 cttgctctgt cactgcatta ctgatacctg ttagattagc taataaatct gataaagatt
      481 gagcatctgt agaaatctct gtatcagtat ttatcgtgat tcctttatct tcatttacaa
      541 gcttccaagt aggtggtata gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta
      601 tcgtcttccc agtatctact tctgtctcat tactcttaga tgaatcgatt ctttcatcca
      661 tcgtctgctc cgtaactgct tctgtctcat tattctcacc ggaaactctc ttcacaccca
      721 aacttatata ttgtccatct ttagtgtgca tagttaaaat tcccacgctt ttactaataa
      781 attcactata accagttctt tcaatagaga cttcaatgta tttcacacta tcatactcaa
      841 aagaaaaata gacatgtaaa agcattttgt tgtttacatc tacataataa tcctttgact
      901 ctacttgaga ccttcctata cggaatacag ctttactgtc tgatatctct ataccagcat
      961 ccttattatt aatctgccaa tctccaagta gctcttctgg caagctagta gtttcttgtg
     1021 aacactttac cacttcactg gtagaggtcg aaacattcac ctccgcactg ttagaagaat
     1081 tttccaagga atcaggagcc ttagcacaac caacaagtct ttatatattg tgcagccgat
     1141 attttcttca ttcagtacac ccttgaaata tgcagtgtta gataaagcag ttcgtcctga
     1201 tttgctgagg cacccatctc ttcttgaacg tactccttga ttttcaaggc cgcccgatac
     1261 tctttcggaa aacgctgctt cactgaagcc agcaaaaaat catccttctc cggcaactct
     1321 tccttcttaa tcattcgggc aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc
     1381 atcgaatctt tatcaacctt tttttcggga aacagatatc ctagcatagc caagatatcc
     1441 tttagtgctc ttaccgagag catggtttct tccatatttg cttgttcact ttgggcattt
     1501 acaatgtgaa acgcaatgtt tccagcctcc tcttctggca actgaacatt catttttttg
     1561 ttgatcaatt ggatagcata gcagccgatc tcaaattctt ttgggtaata cttttgaatt
     1621 tcaatcagca ggcgattttg aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc
     1681 aagtgatcaa ccaaggtaaa aaatagactt ttattcagct taaagtcaaa cttggcgttg
     1741 gcataatcga taattcgctt gacgcaaata attagatcat ccggagcagc ctcaaacagt
     1801 ttaatgtaat ccttttttag agcatcagaa agtgaatcta atttaaatac tttttggatt
     1861 ttgtcagccg gaaccaaatc gcctttcttt agttgaaatc caatcccttt ccccatcaaa
     1921 attacttctt tgccttgctt atctaagcaa agaattaaac tgttgtttaa cgttttaatt
     1981 actctcatta gattcaccgt tctctctaag ctaagccatt tcttgcgatt actttttgat
     2041 accaaaagaa gctttctttt tttattcgtt ttaattcttt aagattctca tcggttcggt
     2101 caacatatat aaaaccataa cgcttattga agccttgatg ggtactaaca acatcaatcg
     2161 ctgaccaagg taaataacca aaaacatcta caccatctgt taaagctgct tgaatagcca
     2221 gaatatgttt ggctagataa tcaattcgat cctggtcatt aattcgtcta tcttcctcaa
     2281 ctatgtcggg tgccccatag ccattttcag taattaaaat aggtaaatca tagcgatcgt
     2341 acaatttcct caatgttaat cgtagtccag tagggtcaac tacccagcca tatggcgttt
     2401 tatctacata aggattttca gctgcccggt agacaccttc ttctcccaac atgatctgtt
     2461 gatcccccgc tctagcggaa acatctgatc cgtcattttt gctggcagcg atggtggcag
     2521 ttgaataata attaatcgca ataaaatcgg gtttaccgga ggataaaatt gcctgatcct
     2581 ctgcctcaat tttaggatat aatccacgat cattcaaata gttttcaaac aaataattgt
     2641 attttccccg aactgctaca tctaagaaag accagcctct caatgtttcc caattatggg
     2701 ctgcaattgc atcttctgga ctggaggtag cttgatacat agccgttaaa ttgattgctg
     2761 gaccgatttt tcctttaaga ttcaactgat gatacaagtg aattactcta gcttgagcta
     2821 ataaaacatg gtgattaatt tgaaagagtt ctttttttga aggcagctct ccacttggtg
     2881 gcaaaccaat cgctccagga tgaaggatca tcgtgttttg ctcattaatt gtcagccaat
     2941 agttcacctt gtcaccatac tcagtaagca atacctgtac atagtccaca aaataatcga
     3001 tagttgctcg atttaaccaa ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt
     3061 acatggtgac aatcggttcg attccctgat tcaggcattc atcaattact tgatgataaa
     3121 aagaaattcc cttttgattg gttttattat tttcaatatc cataattcgc gtccaagcga
     3181 tggaaaagcg aaaagctttt aagcctaatt ctttaaacag ctgtatatct tctttatagt
     3241 gatggtaaaa atccgaggca acttcaaaat tagctacccc ttctgtatgc tgatacttgt
     3301 caataataga cggttgtttc ccatcctcct gataggcacc ctctgcctgg taggcactaa
     3361 tggaggcacc ccataaaaac ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa
     3421 tcctctcctt aactaatttg catcaatggt tcgtgttcct ttaagcttcc gcttgcttca
     3481 ctaattcctg cgtaattttc actgttggtc actaccacaa tcactgtatc atcgtagcca
     3541 gcgtttctaa tggcagcgaa atcggcgttg attaataatt gtcctgctga cacctgatct
     3601 cctgctttta caaatacttc aaagggttcc ccttttaaat tcacggtgtc gatccccaca
     3661 tggatcaata attcaatacc ttctttagaa cgtagaccaa tcgcatgttt agattctaaa
     3721 atagtaatga cttctccctc aacaggtgaa taaatgctgt tttcttctgg atgaatggct
     3781 acgccctttc ccaatagtcc cttagaaaag acagcatcgt tcacttcaga aataggacac
     3841 acggttcctt ttaccggatt ttcaacaatg actgcttcgg ctattgccgt ttgctctgat
     3901 tcatcagaag tatcgtcaaa tccaattaga taagcaatga taaaggttgc aacaaaggcg
     3961 attacacacg taataattgc gtgaacaata ttcattggat tttctccaat aaaggctggt
     4021 aaggctgcta agccagggga aacaaaggca tatctgacta aaccgctcaa gccagcatac
     4081 aaaccagcaa ttgcaccacc aatcatggaa gcaactaatg gttttttcag cttcaaggtt
     4141 actccataga gtgaaggctc agtaattccc attaaagcag tgatcccagt ggacacagct
     4201 agctgcttca actctttgtt tttagttttc gcggctacag caaaggtagc acctgattga
     4261 gcaatattag aagccagcat tcctggccca ttgatcattt cagaacccgt actgctcaat
     4321 tgcatagtag cgattggcgt cattgcccaa gctgtacccg taaccaccaa aaatggctgc
     4381 aaaccaccca tcaaaaatgg aattaaccaa gctgctttgc cgttaatcca ttcagccccc
     4441 attgctataa gatcgttcag caacgttcca aacggtccaa tgacaaccaa cgctaaaggc
     4501 cccataatta aaaataccag catcggctta gtaaagaatt taataaacga aggcgaaatt
     4561 cgatccgcaa agggctctac ataggacatg atccaaacag taaagataat cggtaaaact
     4621 gatcccgcat aatctgcagc taataccgga attcctagaa aagaaacggc atcgccagta
     4681 gaaaaaagct gtgaaatatt tggatgaagc aaaatgccag caacagtaat cgccaaaatt
     4741 gggttgacat caaactttat ggctgcccca taggctaata gtactggcat aaaataaaag
     4801 gctgcatcag caatagtatt caaaatgatg taagtttgac tactgtctga tagtacacca
     4861 gtcaaagtca agacggccaa aatggctttt accatcccgg cacctgttat agctggaatc
     4921 atcggtgtaa aggtggtaga aatgacacta atcaatcttg aaataatact ttcttgattg
     4981 tctgaagagg ggcccttttt aattgaagtt cccacttcct tttgaatttc cttgaaggct
     5041 gtttggactt cattgccaat caccacttgg tattgtccac cgctatccac cacactaatt
     5101 acaccaagtg ttttttctaa ggctttcgta tcggctttcc ttttatcttt gagttcaaaa
     5161 cgcagtctgg tggcacaatg ggttaagctg accacatttt cttttccccc aacctgttgc
     5221 aatacatttc ttcctacttc tctgtaatcc attattctct ctccttaaag cagtaaaaaa
     5281 gaccaagtca aaccacgccc caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg
     5341 cataatcagt aacatgccaa taacgttatt ttgctgtaat ccaataatag cacattatga
     5401 tagcgcaatc aacagggaat ttaatttttt atcttttcgt ttgaccgtat agggtaatat
     5461 aacagttaga tcacatcaac tagggtatat ctgcgtatac ttactgctaa aataagtata
     5521 tctaatatta actataccag taaaaccatt cctacataac cagcgggaaa ttcccaaaat
     5581 cgtgaaagca tcaaaaatgc atataacgga tcacacaact tcaacaattc tgctcttggg
     5641 aaaaattgat gaatttggtg agtaaaggat ggaataaaca gcattccaac aatataggca
     5701 acaatagtga tacttgtttt tttggtaaaa atcagtaagc aatagccaat ttcaatcatt
     5761 ccgagatata aaggtatcga tagaatgatt tgggctagta ttcttaaaaa ttaactagca
     5821 aatgtttaaa ctgatttatt
//
LOCUS       DACESX010000147         5727 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.148, whole genome shotgun sequence.
ACCESSION   DACESX010000147 DACESX010000000
VERSION     DACESX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.148"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            196..921
                     /locus_tag="IXF88_002417"
     CDS             196..921
                     /locus_tag="IXF88_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509125.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="IXF88_002418"
     CDS             911..1420
                     /locus_tag="IXF88_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509126.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="IXF88_002419"
     CDS             complement(1490..2938)
                     /locus_tag="IXF88_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1509127.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="IXF88_002420"
     CDS             complement(2938..3654)
                     /locus_tag="IXF88_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1509128.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="IXF88_002421"
     CDS             complement(3635..3985)
                     /locus_tag="IXF88_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1509129.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="IXF88_002422"
     CDS             4129..4902
                     /locus_tag="IXF88_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1509130.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="IXF88_002423"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="IXF88_002423"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1125 c          994 g         1946 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatactcttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DACESX010000148         5583 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.149, whole genome shotgun sequence.
ACCESSION   DACESX010000148 DACESX010000000
VERSION     DACESX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.149"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(168..1460)
                     /locus_tag="IXF88_002424"
     CDS             complement(168..1460)
                     /locus_tag="IXF88_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1509131.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1457..2080)
                     /locus_tag="IXF88_002425"
     CDS             complement(1457..2080)
                     /locus_tag="IXF88_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509132.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2083..2919)
                     /locus_tag="IXF88_002426"
     CDS             complement(2083..2919)
                     /locus_tag="IXF88_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1509133.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2936..3823)
                     /locus_tag="IXF88_002427"
     CDS             complement(2936..3823)
                     /locus_tag="IXF88_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1509134.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3839..5122)
                     /locus_tag="IXF88_002428"
     CDS             complement(3839..5122)
                     /locus_tag="IXF88_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1509135.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
BASE COUNT         1768 a         1232 c          923 g         1660 t
ORIGIN      
        1 gttattttta ttttctccta tctaaaaaag cagcccacat aaaaagaagg catgacaaaa
       61 agcatgtcat agcccctttt tccgaataaa tagtgttcaa aagacagcct atcggcaata
      121 aggcaagatt ttataaacta cgggaagtaa tttccgtaaa tctattctta ttcttttaag
      181 ctgacaagct cgtcaatcac tactgctttt cctgaagcaa tagaatgatt tgctgcgatt
      241 cctgtcaaaa tcgacaaaat accatctacg tgagttgctg cccgattcca cggatcttct
      301 tctttttctt caccaaatag atcatctaat aaacgcggat ctcctccgcc atgtccgccc
      361 gcttcttgtt cgacgattac ctcatatggc tcaccgaaca tcggatagac atggatggac
      421 tgaagaacgg ctgctccttc atccttttta tctccacctg cattgatgta agacttttcg
      481 atcactttca tttcgatccg tcctttgctg ccattgaaag aaacattgaa tccttcccac
      541 ggcatatacg cgtttagcga atacgtcaaa atcgcaccag tttcatattt gaccgttacg
      601 cctagtgtat cttcaataga gatatcatcg gaaaatacac tgcggtcacg aatatatcca
      661 ctgtcttttt ctgcttctaa atacatttct cgcaattcct cattggctga caggtctata
      721 gcaaatggat cagctttagc tagttcgctt ccatagcatc gactgtaaaa tatttctact
      781 ccgcgtttct ccgcattatg catcccataa aagtttaatc caccgaatgc aaataccttt
      841 tggggacggc tgttcaacca aaaattcact agatcaaaat gatgggtaga tttatgaaca
      901 agaagcccgc cgctattgtg cttatttcga tgccatcttc tgaaataatc tgccccgtgt
      961 tgcgtattta acagccattc gaagtggata gaaaacacat caccaatcac atcactttct
     1021 atcagttcct ttatcttcgt gtgatacggt gcataacgat aattaaaagt gactcgcagg
     1081 cttcttcccg tctgtttttg gaccgttatt atttcttgag ctttttcctc atcgatagtc
     1141 agaggttttt cgcaaataac atcatatcct tttttcattg cacgaatgat ataatcatga
     1201 tgcgttcgat ctactgacgt aataatgact tcagccggcc gtacgtcatc cagtagttgc
     1261 tcaaattgtg atggcataca tgtctttact tttttctgtc catacttgtt cacaagtatc
     1321 cgatttgcat aatccatcct tgtttgagat acatcacaaa aaccgactag ttcacaagtt
     1381 tgatgatacg tagaggcaat tgcctcataa aaaaaacgcg ctctcccacc aagtccgact
     1441 tgtacatatt ttttcttcat ttaaagtcat tctccttttc ttttaagcaa aatactgtga
     1501 caaatggcag ccctggatac agtaatgcag tgagtaaaaa atcatgctgt atacaccaga
     1561 acagtccata caaaaggcat aaataccaca gaaacccctt gacgtttttt gacaacagcc
     1621 agatattcct tttgatcaaa taaacaagac tatctgcttt atcttctttg cagatatcaa
     1681 ttagcgtact aaaaaaacta tgggatacaa aaagcaacag acacaataga aaaggaatca
     1741 gataccaagc tccaatcttt cccactaata aataccaatc aactgcaaaa ataatgacaa
     1801 acagctgaaa caacgtaatg atgaaacaca tccgtttgat ttttcctttc ctccaacata
     1861 cctttttttc tgccaagtaa tggacagata gataataaga aggatacatc atcaaaaaat
     1921 aaatcagata gacaacacga gcagacagca ctaatcctaa agtgaacaaa catcctagaa
     1981 ctaaagcgaa aagagcatta ctgataagac tggttaaaaa aatccgacag acatagtagt
     2041 tgagcttgta aagaaacatc tcttctattg gctttttcat attcagccct tcagaccgct
     2101 tgtactgatc ccctggacaa gatatttatt gaagaataag aaaatcagaa aaaccggtag
     2161 cagcgacaac gtagacatcg caaacatact cgcgtaagtc gtactagatc cagcgtctgc
     2221 aaataatttg atggcaatcg ataccgtgta agatctcggc ttgtttaaat aaatcaatgg
     2281 tcccatgaag tcgtcccatt tccaataaaa ctggatgatg atcgtcgtga tgatcgaagg
     2341 tttcagcaaa ggaaaaataa ttttggtaaa gacactatac ttgctgcatc cgtcaataat
     2401 cgctgattca tctaactctt tggggatgct gaccatgaat tgcatgatct ggtaaatgaa
     2461 aaatgcttgt ccaaaaaaat gtggtaaaat cagcggcaca tacgtcccaa ccaaattcag
     2521 ccgattgtaa ataataaact gcggaatcaa aatcacttgt gcaggaatca tcatcgttac
     2581 taacatgacg acgaaccaga atctttttcc tttgaaatcg attctcgata atgcgtaagc
     2641 aatacaagtc gagctcagaa ctgtccctat cgtcgaaact gcagagatga caaacgaatt
     2701 cctaaagaaa gtaaaaaatg aaattccagc aaaccccgcc catccttttg cgtaattttc
     2761 ccattgaaaa gtctctggaa tcaaattcag cgaaccactc ataatctctt gatccgtttt
     2821 aaaagaacct gcgatcatcc ataagaccgg ataaatcatg actagaccta atagcaaaat
     2881 aaaaccgtat tgaaaccatt tcgttactgt attattcatt tcttcctcct ctcattcaca
     2941 caacgaccca tttttttgat gatttgaaaa tcagaaaggt gacagctccc ataatcaaca
     3001 gcatgatcca cgaaatagca ctagcatacc ccatagaatt aaaagaaaaa gcttggttat
     3061 acacataaag agcataaaag ttcgtggcat cattcggacc accttgggta atgataaatg
     3121 cctgcgtaaa agtcataaac gccgcgattg tctgcataac caagttatac aaaatgatcg
     3181 gcgaaagaag aggtaacgtg atcttgtaaa acgtctgcca tttgttcgct ccatcgatct
     3241 ctgccgcttc ataatacgag cctgggatct ctttgatacc tgcagcaaag atgatcattg
     3301 ccgaaccgaa ttgccaagca gacatcaaaa caagagggat catgaccagt ttttgatcgc
     3361 taaaccagga aagcgcaggt gcgcctaatc gttccaggaa aatattcact aacccgtttt
     3421 tagaaaacaa ctcccgccaa actagagcga tagaaatact gccaccgatc aaactaggca
     3481 cataatacaa agaacgaaag aaagtcgttc ctttgatatt cctcgttaaa agataagcaa
     3541 tcaatagacc aaagatcagt ttcacaggta cagatgcaaa tacatagaga aaagtgactc
     3601 ggatggagtt caaaaaacgg gcatcactca gcatctttat ataattatcc aagccgatcc
     3661 atgagactgg atttcccaaa cgataatcgg tgaacgaata ataaagtgac aagatcatcg
     3721 gtatcactgt tatcaataga aatcctagga tgaatggaga agcaaatacg taaccgacca
     3781 cattgtcttg atccaagaac cgttttagtt tttctttttt catgccttcc tcctgctgct
     3841 atcctagaat gtttgacgca aactcataaa gactctctgc tgcttcttct gctgttactt
     3901 cttcataaat gactttttca actaaaagat tgtattggtc ttgaatttcc ggattcaaag
     3961 cactatcaaa gacattcact tcgccgttgt ccatatcgga aatcatttct aaataatcaa
     4021 atgtcgcttg caaagttgca tcatctgcac gagcgagctg ttctcgaata ttcgacatga
     4081 ttggtacacc acgttctcct tttaaaattt cattggcttc ttcactgttt tgaaagaaat
     4141 caataaactt agcagcttct tctttatggg ctgagctttt tgccatactc agcatttgcg
     4201 aagaacgtaa agggatacca gattctccat ctgcttttct gcgaggtgga ttaatcaact
     4261 gaatctccat atcttcagga gcccctgaca atatttctgt caactggtta cttgatagaa
     4321 acaccattgc cgcttctcta tccctaaccg cctgaaaaga cagatccgtc gtacttgcag
     4381 ctccaccacc tggttctgga tgggcccctt tgtctaccag atttttacgc agctggaaaa
     4441 aatctgttag atagtctgga ttttcaaatc ccaagctgtt agatagatct tcccgattat
     4501 aaaaattcaa tcctttctca acttgtggta tttgaataat acagccagct ataaaatctt
     4561 cgaatcttga aatagcatag attcctaatt cttcagtgat ttgatagctg atttgttcaa
     4621 attcatccca tgtccaattt gaagctggtt cagggatatt taattgttca aatatatttt
     4681 tattgtaagc aatcgcaaac gaattcaccc cgttagacag tccaactaat tgcccgtcgt
     4741 aagtcgtcgt tgctaaatag gattcatctg cattagaaat atcaattatc ccttgctcca
     4801 catattcatc taatggttca atctgattga tgtattgagg aaattgattg ttcagttgga
     4861 aaatgtccca gatatcatct gatgctgcta gtgtacctaa ttttgtgtaa tatccttcat
     4921 aatcataaaa ttcataatcg atcgtcacat ggggattttt ttcttcataa agctcgatta
     4981 cttttactgt tgcatcgtgt cggacttgcg aaccccacca agccatgttc agcgtgattt
     5041 ttttgtcatc ttcttgccca caagccgtca agaagaataa cataacactt aaagctaaga
     5101 tgattctcat tcttttcttc attctctgca cctctacttc cattgttatt tttcaatata
     5161 ctgtattttt atacagaaca tatttattaa acgatctttt tcaaggggaa taaaaaccaa
     5221 gacttgcctt atcgttctgc ctaggatgaa caactcaata ctttcaagaa aaattagata
     5281 gtgctgattc ttctttcatc gctaaaaaca cttcttgctt caatgatggg aggataatta
     5341 cgcaaagaaa aaaggatatc agacaactgc ccaatatcct tgacatagct aaataagcat
     5401 gcttcttcgt gtgcgctctt ccctaaaaaa cagaaaaata actagtaatt ctgtatgaaa
     5461 ttaggtacaa tatacacgtc gatgcacaag cattgactat ggcaacttga ttggtcagtt
     5521 cccgctgact tttcaagccc cgacaattta gctgctacta aattgtcggg tgcctttttt
     5581 agt
//
LOCUS       DACESX010000149         5577 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.150, whole genome shotgun sequence.
ACCESSION   DACESX010000149 DACESX010000000
VERSION     DACESX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.150"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            65..766
                     /locus_tag="IXF88_002429"
     CDS             65..766
                     /locus_tag="IXF88_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1509136.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            763..1884
                     /locus_tag="IXF88_002430"
     CDS             763..1884
                     /locus_tag="IXF88_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1509137.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1899..3131
                     /gene="pepT"
                     /locus_tag="IXF88_002431"
     CDS             1899..3131
                     /gene="pepT"
                     /locus_tag="IXF88_002431"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1509138.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3263..4171
                     /locus_tag="IXF88_002432"
     CDS             3263..4171
                     /locus_tag="IXF88_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1509139.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4206..4478
                     /locus_tag="IXF88_002433"
     CDS             4206..4478
                     /locus_tag="IXF88_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1509140.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4489..5535
                     /locus_tag="IXF88_002434"
     CDS             4489..5535
                     /locus_tag="IXF88_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1509141.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1813 a          970 c         1211 g         1583 t
ORIGIN      
        1 tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta gaaggagtga
       61 agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact ttgtccctga
      121 atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg cattgatatt
      181 aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt atgaatcagc
      241 acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc ttgcaaatgg
      301 tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg gtatgggagg
      361 ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa gcggacaaga
      421 acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc tgcaagaaaa
      481 tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat atgaaatcat
      541 tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga tgttcgggcc
      601 atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag agcttcaaca
      661 aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac gtcaaaaaca
      721 aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat cagcggacgc
      781 actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga aggagaccca
      841 gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat gacattagac
      901 gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct gattgctaaa
      961 catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca agaaaaaatg
     1021 tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa catggatatt
     1081 atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt ggagagttat
     1141 ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc tgtggatcat
     1201 gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg tgattatacc
     1261 ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg tgcacaacca
     1321 gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga ggttattttg
     1381 ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc ttatgaggaa
     1441 cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt aggacgagta
     1501 ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa agaagcgttc
     1561 caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt gaagcgcatt
     1621 gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca aagagcagat
     1681 gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag cgctgggttg
     1741 attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt tatcgagaaa
     1801 tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc tgaaacaaat
     1861 acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat cttttgccac
     1921 gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa acgactccat
     1981 cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa gagttaggct
     2041 tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta ccaagcaatg
     2101 ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca gattttaatg
     2161 cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg atcgtactag
     2221 atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa aattatgaag
     2281 gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac aaatcaggga
     2341 ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc cctcacggta
     2401 ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat aaattcgatg
     2461 ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta ggtgagttgc
     2521 agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa aatgtacatc
     2581 cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat tttcataatc
     2641 agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc ttccatttga
     2701 tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc gatcatgatc
     2761 gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag ttgaatcagc
     2821 gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac atgaaagaaa
     2881 tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc gagcttggta
     2941 tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc tcttacatgg
     3001 gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga ttcgaatttg
     3061 tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca gaattgaaca
     3121 caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca atgatggaat
     3181 gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc ctcttttttg
     3241 aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca aaagttcagg
     3301 gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag aaaagcagcc
     3361 agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg aattagtcaa
     3421 gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg gcacgattca
     3481 tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat tacttccagg
     3541 tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag aagaagctgc
     3601 tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca atgatgaagt
     3661 cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga tcagtcaaga
     3721 agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt tattgctgaa
     3781 gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag gaaaatccca
     3841 actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg acgactcagc
     3901 gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata ttttccgatt
     3961 tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg gcattactta
     4021 tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca ctcgaacgga
     4081 tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag gattaacagt
     4141 ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa gttggaagaa
     4201 gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt gtgaattttt
     4261 acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc tggcgtgttc
     4321 ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga ttgatctctc
     4381 gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc gctgcaggtg
     4441 tacttgttga tttgattctc atttattttt tccattaagg agcaggagat gtcgtctaaa
     4501 ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt tgccattatt
     4561 tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc taccagtgtt
     4621 gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga agggcgctca
     4681 atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg gttcttgacc
     4741 tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct gatctcggag
     4801 atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt gacagcactc
     4861 catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt ggtcatccaa
     4921 ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc tggtatcctt
     4981 ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa tgttcaaata
     5041 ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa atgggtggat
     5101 acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat ccaagacagg
     5161 actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg gtatgtactt
     5221 ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac catttcgttt
     5281 atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat tggttccttt
     5341 gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc agtttcttcg
     5401 cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt tgtgtatgtg
     5461 attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa agagaataaa
     5521 gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa agaaaaa
//
LOCUS       DACESX010000150         5464 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.151, whole genome shotgun sequence.
ACCESSION   DACESX010000150 DACESX010000000
VERSION     DACESX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.151"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(104..2008)
                     /locus_tag="IXF88_002435"
     CDS             complement(104..2008)
                     /locus_tag="IXF88_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1509142.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            complement(2206..2439)
                     /locus_tag="IXF88_002436"
     CDS             complement(2206..2439)
                     /locus_tag="IXF88_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAQ1509143.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            complement(2586..3440)
                     /locus_tag="IXF88_002437"
     CDS             complement(2586..3440)
                     /locus_tag="IXF88_002437"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1509144.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            complement(3425..3763)
                     /locus_tag="IXF88_002438"
     CDS             complement(3425..3763)
                     /locus_tag="IXF88_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509145.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            complement(3991..4608)
                     /locus_tag="IXF88_002439"
     CDS             complement(3991..4608)
                     /locus_tag="IXF88_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1509146.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            complement(4840..5412)
                     /locus_tag="IXF88_002440"
     CDS             complement(4840..5412)
                     /locus_tag="IXF88_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1509147.1"
                     /translation="MTENKATQERTPQERLQEEQEHNEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
BASE COUNT         1533 a         1265 c          932 g         1734 t
ORIGIN      
        1 ttcaagaact caaaaaagcg gaaaatccca gatttaaaaa tctgagtttc cgcttttgtc
       61 atttcaagga tggactattg tctcagcctc ttctaattta ttcttaagat atttttactt
      121 tctctgcagg tgctgatact tctttttgtt cttttcgacc tttcaactta tacttcatta
      181 atctcatgcc attgaaaatt accaccaaga tactaccttc atgaaccaac attccgatag
      241 acatattcat ccattcgctg aagaagacgc tggtcaacaa gaccaacact actccaattg
      301 aaatcacgat attttgtttc atattatttg cggtggcttt tactaatcct aatgcgtgag
      361 gcaagttgct gaagttcgag ttcattaaaa cgacatcgga tgtttcaatc gctacgtccg
      421 ttccgcttcc cattgcgatt ccaatgtctg ctaaagctaa ggacggacta tcgttaacgc
      481 catctccaac aaaggctaca atttgaccac gttgttgaag ttttccgata taagccgatt
      541 tgtcttccgg caacatgtag ccgtgagctt cggtcaaacc aagttcgccg gcgaccacat
      601 caacggttcc ttggttatct cctgaaagaa cgactagatt tttcacgccc aagtctttta
      661 attcctgcaa attagctttc acacccggac ggacttgatc gcgaatgccc atcagtactt
      721 ttaattcacc gtcgaccgct gtcaaaacga gagagttccc ttgttgttca aaccgtttga
      781 catctttttg cacttttttg ctgagagtga cattttcttt ttccatcaag gccacattcc
      841 caacagccac tctatgtcca gctacacttg aaacgattcc gccgcctttc acaacttcag
      901 ttccttcaac aggataaaag tttgtttctc caatttgatt taagaccgct tttgctaatg
      961 gatgatctga ttcccgttcc acactggcca gatagccaag cgtttcagca ggatcttttt
     1021 catacagttc agtcgctgca acggttgggt ttccgacagt taaagtacct gtcttatcaa
     1081 atacaattgt atccacatta ctgaagtctt gaatgacttc actccctttt aaaagaacac
     1141 cgttacgagc accattccca ataccggcaa cgtttgagac aggcactccg ataactaatg
     1201 cgcctgggca acctagaacc aagatggtga ttgctaactc aatattttga ctgaacgccc
     1261 atacaacaat tgccagaacc aagacagctg gtgtgtagta tttagaaaag cgatcaatga
     1321 accgttccgc ttcggattta gaatcttgtg cttcttccac gagttcaata atttttccaa
     1381 atgtggtgtc ctcaccaact cggtcagctc tgatttgaat cgttccgttt tctaaaatgg
     1441 ttcctgcaaa aacttctgca tcagctagct tgttgaccgg aatagcttca cctgtaatgc
     1501 tagcttcatt gatatgccct tcacccgtaa ggactatgcc atctactgga actttcgcac
     1561 ccgttttaac gagcaagata tccccttcat ctatatcatc cacttctact tcttcaaatt
     1621 cgccattatc catttgtttc aaggcgcttt cgggtgccat ttcagtcaat tctttgatag
     1681 cagatcgcgt ttggttcaat gttcgttgtt ccaagtagtg tccgaataag aataagaacg
     1741 tgacaatagc cgattcttca taattttgaa taaaaagcgc accaatcgct gcaatactga
     1801 ctaagacatc aatactgatg actttgactt tcattgcttg aaacgcttga atggcaattg
     1861 gcgtaatccc aaagacagag gcgatgatca aagaccactc tgaaagtcct acgttttcta
     1921 aaacaaagtg actgaagaaa ccgagtacga ttaacaatcc actgatgacg gtgataacat
     1981 ttttcttgct taatatatat tgttgcatgc tttttccctc caaatttaat ttctctttta
     2041 ttgataaaca cagtataaga taattgccta agtaattaat tgactgacat caagtttaga
     2101 aagttctctt ttatcgtttc taagcacctc gaatcggatt gcaacaaaaa aagcaatcct
     2161 ggaaaaatag cttttcttcc agaattgcct agttcattta atctgttaaa cggccttgac
     2221 ttttgcttta agaaccggat agcctacgtt ttcgatagct ttttgaatct cttcaatcga
     2281 agtgacagct gaatcaaaat tggttttgac tttgctggag ttgaataata ctttaatgct
     2341 gtctttatca ataccgttaa ctgatttcac ggcaccttca attttttgca tacaacttgg
     2401 acaagacaac gtttctaatt gcaatatagc tttttccata attcctatct cctttatgtg
     2461 tttttatttt atcggcagca gattaatgaa cactgccttt tcctctttac aactgtagta
     2521 tagctcactt ttagcaaacg aaaattgacg catatcaagt tttgcacttc tttttatctt
     2581 tttgctcaat tcaccatctt aatagtcttt ttctgcccct tattgccatc agaataataa
     2641 gaaattggtg gataatcttg tctgcgtata aaccatacta caatcgcgcc aataaacagg
     2701 gccagagaca aggcttgcga aacgcggatc cactcgccaa tccataaact gtccgttcgc
     2761 ataccttcaa tgaagaaccg acccactgag taccacaaaa cgtaactcaa agcgacttct
     2821 ccttgacgca aaagctgttt tcggttccgt atagtgacga tgaggacaaa gcccaacaaa
     2881 ctccacaatg actcgtataa aaaagtcgga tggtagtagg taccattcat attcatttgt
     2941 tcaatgataa attcaggtaa ataaagattt tccaagaatt gacgagtaac cggcccacca
     3001 tgagcttcct gattcataaa attgccccaa cgaccaatgg actgggctaa caatacattg
     3061 ggcgctaaaa tatcaagaac gagtgccaaa gaagtacctt ttttctttgc ataccaatat
     3121 agcgtacccc ctcctgcaat caatccccca taaatcgcaa tgccgccatt ccaaatcgca
     3181 aggatttgtc ctggattttg cagataataa ccaagttcaa ataacacata ataaagccga
     3241 gcgccgagga gtccaatcgg aattgcccat aaggccatat cgataatggt atcctcttcc
     3301 agtccttttt tctttgcttc cttggtgctc aactggagag ccgctaatat gcccgctgca
     3361 ataatgactc cataccaatg aatcacaaca gaaccaatct ccaccacatg gggattcaaa
     3421 acgcctaata cgaatcccat tctatttgct ccatttcttc aatttttcgc gtcattctag
     3481 tcatggtgat gctgaacaag attccggcta agctccatgg aaaccaaaca gtgaatacac
     3541 ttttatctcc ctggtcactc gcttcgtaat acgccacttt tccgtgtccg attacgccgg
     3601 ctccttttac aacttcctct tctttttcca tagctaacct atctgcctca acaacgttag
     3661 acgtttttgc ttcgccgcca atcgtgttct tggtgaaagg gacgacaaag aatgcccccg
     3721 ctgctgacaa cgtgatggca actatcaccc aaaaacaatg cacattcacc ctacttattt
     3781 ttacgttttg ttcgacaacc ctatcttaaa gaaaaataaa acaaaaaaac ttgagggtta
     3841 tcatgtttgt gcttttattt ttccaatgaa tgaaggtcgt tctgtcttaa aaaagccaaa
     3901 aacggacaga tggaagcaaa agcactgagt tctgtcccgt tgttaatgaa gagcgacaga
     3961 acaccaacgt gtagaaaaaa gtgtgtccga ttattcgctg taaagcaata aatcatctaa
     4021 atcctgaatc ttgatccttt tcccagacag ctgttgaatc aatccttcgt cctccaattc
     4081 tccaaatttg cgactgatgg tctcaggtgt cgttcctaaa taggaagcaa tgtccttttt
     4141 tgccatcggc agggtgatgg tgggagagtt gcccatttca ggttccacat tttctgccaa
     4201 aaacaaaact aaacgggtaa tgacactctc cacggctact tgtgtcgttt gacgttctga
     4261 actttctaac cgtttagaca tttccgccaa tagttttaaa gaaatagctg ggtattgttc
     4321 cagaaaatct tggacatctt tttgctgaat catacagaca acggtatctc gggttgcttc
     4381 ggcatattcc tcgtgtactg catccgggtt gaacaaggtc cattctccgg tgaaatcacc
     4441 agggttcaag atccgcacta gctgctcttt gccagagtct gccaatcgat aaatacggat
     4501 tttcccacga ttgataatat ataaagtatc ctctttttct tgtgaacgaa acagaaactc
     4561 tcctttttga tactgtttcg tgtgggcttt ttcagcaatg taattcatcg cgctatcctc
     4621 taagtgattg aatattggaa ccaagcggat acaggctgcg tggtcgccaa tttcatgatg
     4681 ggtatgttta gtcaattcat tctccccctt tatttatttt gtttatattc gctgctttta
     4741 ttatataagt ctcttactac aaaaaaattg atgcacatca agaaaatgga aaaaggactg
     4801 gaaagaattc caatccctaa atcagcttct acttttacct taatcttcat cctcatcaag
     4861 agcgtcatca tcttctaatg cttctttacc aatgaacgct tgcagcatcc aaatgttctt
     4921 gtctaagtca tttttaaaag caatcaatgt atcttctaaa gcatcgtcac cctcgtcttg
     4981 agctaaacga atcgcgcgaa tagttaggtc acgagtgctt ctgaagtcct cgataatatt
     5041 ttcaaccatg tcttctgctt tagcgtattt attaactgca tcttccgaaa gcatcgaata
     5101 tttttcaaat tcaaccgttg tcgaagcagg tttatgccca gaagtaatta gacgctccgc
     5161 aagtttgtcg aaccattttt cattttcgtt atacaactct tcaaattttt catgcaacgt
     5221 aaagaaattg gctcctttta cataccagtg atattggtgc agtttcacat gtaatgtatg
     5281 catgttgcct aaaagatgat ccgcaatggc cgctgcatta attttcgtat gatgaacatg
     5341 ttcattgtgt tcttgttctt cttgcaatct ttcttggggt gttctttctt gagtagcttt
     5401 attttcagtc atgatttatt cctcctataa ttttggtttg tttaaaatag ctttctttga
     5461 ttcc
//
LOCUS       DACESX010000151         5460 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.152, whole genome shotgun sequence.
ACCESSION   DACESX010000151 DACESX010000000
VERSION     DACESX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.152"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            7..825
                     /gene="purR"
                     /locus_tag="IXF88_002441"
     CDS             7..825
                     /gene="purR"
                     /locus_tag="IXF88_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1509148.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1328..2701
                     /gene="glmU"
                     /locus_tag="IXF88_002442"
     CDS             1328..2701
                     /gene="glmU"
                     /locus_tag="IXF88_002442"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1509149.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2795..3766
                     /locus_tag="IXF88_002443"
     CDS             2795..3766
                     /locus_tag="IXF88_002443"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1509150.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3772..3874
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3940..4509
                     /gene="thiT"
                     /locus_tag="IXF88_002444"
     CDS             3940..4509
                     /gene="thiT"
                     /locus_tag="IXF88_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1509151.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4590..5363)
                     /locus_tag="IXF88_002445"
     CDS             complement(4590..5363)
                     /locus_tag="IXF88_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509152.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1784 a          970 c         1154 g         1552 t
ORIGIN      
        1 gaataggtga aagtacgtcg cagtgaacgt ctagttgaca tgacacagta tttattgaat
       61 cacccgcatg aattgatttc cttaacaagc tttgccgaac gttatgaatc tgctaaatct
      121 tctatcagtg aagatttggg aatcatcaag aaaacattta aagaacgagg gtatggaacc
      181 ttggaaacaa tcccgggtgc tgcaggtggt gtattgttca tccctgaaat ttcatatgaa
      241 gaggcgaaaa aatatattga atctctagca gaacgtctat cagaacaaga ccgtctgctt
      301 ccaggaggat atgtttatct ctctgatttg ttaggagaac ctgaactgct gcgtcaagtt
      361 ggaagaatca ttgcttctaa ataccttggt gaaaaaatcg atgctgtaat gactgtagcc
      421 acaaaaggcg tacctattgc ccaagctgtg tcctattatc tgaatgcgcc attcgttatt
      481 gtacgccggg actcaaaaat aacagaagga tcaacagtaa gtgtcaacta tgtgtctgga
      541 tcttctgaaa gaattgaaaa gatggaactg tctaagagaa gtttgaaacg tggttcacgt
      601 gtccttgtag tggatgattt catgaaaggc ggcggaacag tcaacggaat gaagagcttg
      661 attgaagaat tcgaatcaga acttgttggc atcactgtat ttgccgaatc aaaattcaac
      721 ggtcatcgcg ccatcgatga ttatacgtca ctgctttatg tcaaagatgt agatactcaa
      781 acgaaaaata ttacggttgt gccaggaaat tatttttcag aataagaggt tgtgaaaaag
      841 ccgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttccaca ttttttcata
      901 aatttggctt aataccgaga aactggcttt tgaacaccgt ttatctagag gttgtgaaat
      961 cagcgtcctg tcctgagtat tagctcaata aacggaaaaa ttgctcctca tattttgccg
     1021 tttattgagc taataccgaa ggtcagctga ttgaaaaacg tttaatgaag aggttgtaag
     1081 cctaccgttt aactctaagt cacaagaaac gatttcaaaa aacagctttt catgttttat
     1141 gagtagtgtg acttgtgacc gaggagttgg caggagagca ccggttacca aatagatagg
     1201 cccgttatgg tctatctatt tttttatcca gaaaacgctg ggataaaaga aaatcacttg
     1261 ttttgttgaa aaaatgggta tactgggata aactgaaatc agcaaactat atttaggagt
     1321 gacgcagttg gacgcacgtt atgcaattat tttggcggca gggaaaggta cccgcatgaa
     1381 gtcaaaatta tataaagttt tacatcccgt ttctggtaaa cctatggtag aacatatcat
     1441 taatcgggtc agtgaaacga aaccagatga agtgattacg atcgttggac acggagcaga
     1501 acaagttaaa gcacaactag gagaacgcag taaatatgcg ttacaagcag aacaattggg
     1561 aacaggacat gctgttttgc aagctgcttc tttcttggaa ggaaaaaaag gaacaacgtt
     1621 agttatcagt ggcgatacac cattattgac tactgaaacg ctgaacaatc tttttgaata
     1681 tcaccaagga aaaaatgcaa gtgcaacgat cttgacggca caagcagaaa acccaacagg
     1741 atatggacga atcatccgtg accatattgg gatcgtagaa aaaatcgtgg aacaaaaaga
     1801 tgcaacacca gaagaagctc ttgtacaaga aatcaataca ggaacttact gttttgacaa
     1861 cgaagctttg ttcgatgcgt taagcaaagt tggaacaaac aatgctcaag gtgaatacta
     1921 tctaacagat atcatcgaaa tcttaaaaga agaaggccat acagttgctg cttatcaaac
     1981 ggatgatttt gaagaatcaa tgggagtcaa tgatcggatc gcgttagcta aagcaaatga
     2041 gatcatgaga aagcgaatta accagatgca tatggtcaat ggcgtaagct ttgtcgattc
     2101 agcaacgaca tacatcgatg caggcgtaga aatcggtcca gacacattga tcgaagcagg
     2161 tgtacaaatc caaggaaata cagtcattgg ttctgattgt gtcatcgggt cacattcaaa
     2221 aatcgtagac agtaggattg aggatcacgt cgtcattgaa aactcagtca ttgaaagcag
     2281 ccatgtcaag aaacatgcag atgtgggacc ttatgcacat ttacgaccaa aagctgaaat
     2341 tggcgaaaac gtccatatcg gaaactttgt ggaagtgaaa aatgctcaaa tcggcaaagg
     2401 aactaaagtc ggtcacttga cttatgttgg tgatgctacg ctgggagaag aaatcaatgt
     2461 cggatgtggt gttgtcttcg tcaactatga tggaaagaac aaacatcata cgactgtcgg
     2521 cgaccacagc tttattggtt ctagcacgaa tatcatcgga cctgtcgagg tagctaaaaa
     2581 cagttcgatt gctgccgggt caacaattac agacaatatt ccagaatatg cgctggcaat
     2641 tgcgagagca agacaagtca ataaagaagg atatgcaaaa aaactccctt atctaaacta
     2701 agacttttgc aaaatcactt gatttattta aaaaatatga ctactattta ttaggtagaa
     2761 ttctagagtt gtgaaaaaga aagtggaggt ccccatgtca aaacattact ttgacccaag
     2821 attaaaaatt tttgcgttaa attccaatcg cccattagca gagaaaattg cagcagcagt
     2881 cggcgtcgag ttaggaaaat catctgttac tcaatttagt gatggagaaa ttcaagtaaa
     2941 cattgaagaa agtattcgtg gatcgcatgt ctatgttatt caatcaacga gcagcccagt
     3001 caatgataac ttgatggagt tgctgatcat gattgacgca ctgaaacgag caagtgcaaa
     3061 aacaatcaac gtagtcatgc cttattatgg ctatgcgcgt caagaccgta aagcacgtgc
     3121 acgggaacca atcacagcga aattagtagc aaacatgatt caaaaagctg gtgcaacaag
     3181 aatgctgact ttggaccttc atgcagtcca aatccaaggt ttctttgata ttccagtcga
     3241 tcatttgatg ggtgcgccac ttattgccaa ttatttctta gaaaaaggaa tcaaaggcga
     3301 tgatgttgtc gttgtttcac cagaccatgg tggtgtcaca cgggcacgta aattagcgga
     3361 atttttgaaa tctccgattg cgatcataga caaacgccgt ccaaaagcaa atgttgcaga
     3421 agtaatgaat atcattggac aagtcaaagg caaaacttgt gtactgatcg atgatatgat
     3481 cgatacagca ggtacgatca cattggctgc caatgctttg aaagaagctg gtgcaactag
     3541 tgtgtatgct tcatgtacac atccagtatt atccggccct gctttacaaa gaatagaaga
     3601 ttcagctatc gagcgtctag ttgttacaga ctcgatctat cttccagaag atcgtaaaat
     3661 cgacaaaatc gatgaagtaa gtgttggcgg actaatggga gatgcaatca aacgtatcca
     3721 tgaaaacaaa ccagttagtc cattgtttga aacaaaaaat aaataataaa taaactcaca
     3781 aaggggagtc caattgggct gagattgaat ctatttctaa acccttcgta cctgtatcgg
     3841 ttatgcgagc gtaggaattg tgaataaaca gctgtttgac tattttcgaa agctgcttcc
     3901 tcctttgtga cttttttatt caaaggagga agttttttta tgggaaaaaa tagagtttgg
     3961 gtagaaggaa cgattgttgc agcgttagcg atggtgttgt cgttgattcc gatccagatc
     4021 ggcagcagtt tttctatttc attagggcag attccattga ctctttttgc gttaagaaga
     4081 gggacaaagg cagggatgct ggcaggtttc atttggggca tcctacattt tccgttggga
     4141 caagtttatt atttaagtgt cgttcaagtg ttgattgagt acccgattgc ctatactttt
     4201 gcaggcgcag cagggctcat ggcttcaaat gtccaaaaag gctatgtaga cgataatacg
     4261 aaacaaatta gaaaaagcat cgtgtttggt gcattgattg gcgcactttc cagatatttt
     4321 tggcatttta ttgcaggagt ggttttctgg ggagcgtatg cactatgggg gatgaattct
     4381 tggctctttt ctttcgtaat gaatggtgcc agcgggttga gtacagctat tgtaacgatc
     4441 atcgttttat tagggatcca acaaacgact ccttcgattt ttgtgccaga taaacgacag
     4501 gagctgtaaa taaaaaagaa gcaagaaact tgagacagaa tcatgtccta gtttcttgtt
     4561 tcctttttat tttttgataa gaaatagatc tacttcaatg gcagataaga aagatcggca
     4621 tcttctacca attgccagtg tcgatcggtg acagagtaga ttaaagtagc gatgatttcc
     4681 tcttctcgat accgggtgcc ttttaatttc caaatataat cttcaatcac atagattggg
     4741 atacgatatt taccatcgat caaaccaact tgatcaaaag aaaaaatgat cccttcctgc
     4801 atcaaattac gcatttcttt caataattcc acactgattt tttcaacagg tgtttctttt
     4861 ttgaaaaaag atttttttac attgatctta tctgacaaga tcgaacttaa tttactagca
     4921 aaaatcctat aaaattcatc taaatagcga taataaatcc gattcagctg atcattaaga
     4981 tcgaagtata caaaattatt ctgtagttta taaaaaaacg gcgaatgaag atgtgttttc
     5041 atatgaccaa aatataataa ttctgaaatt tctaaaggtg tcaattcttt cagcatcgtt
     5101 aaatcagtaa agtcgatcca cttcaattca tcgaatccac gcctgttacg agaagaggaa
     5161 aagaaacgct gaatattttc tgtaccacgg attactttca gccctgtatg ggattcatat
     5221 tctccagcag aagcagatgg atcaagcaag agcaagtttt gaggaaaatg cacgatactg
     5281 tttgcaaagt cagtagcagt cagccctttt gttaaaacag cattacttgt ggtatcaata
     5341 tgtacataaa ctgactcagt catcatgttc ctcctcttat ttcctcttaa tctcttttat
     5401 tttacaacat ttgcattaaa aaagcttgtt tcttttattt taaaattcca ttacaaaatc
//
LOCUS       DACESX010000152         5290 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.154, whole genome shotgun sequence.
ACCESSION   DACESX010000152 DACESX010000000
VERSION     DACESX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.154"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..689
                     /locus_tag="IXF88_002446"
     CDS             <1..689
                     /locus_tag="IXF88_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1509153.1"
                     /translation="LIPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFF
                     AAYSFTYCRIKGKKIWYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFAD
                     AMGIFLLTQTMREIPVSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWN
                     EYVWPVLILRTVDNYTLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYI
                     IGTFVSAGIK"
     gene            705..1829
                     /locus_tag="IXF88_002447"
     CDS             705..1829
                     /locus_tag="IXF88_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1509154.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            1807..2610
                     /locus_tag="IXF88_002448"
     CDS             1807..2610
                     /locus_tag="IXF88_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1509155.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            3061..3333
                     /locus_tag="IXF88_002449"
     CDS             3061..3333
                     /locus_tag="IXF88_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ1509156.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            3317..3652
                     /locus_tag="IXF88_002450"
     CDS             3317..3652
                     /locus_tag="IXF88_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1509157.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(3831..5045)
                     /locus_tag="IXF88_002451"
                     /pseudo
     CDS             complement(3831..5045)
                     /locus_tag="IXF88_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         1670 a          890 c         1051 g         1679 t
ORIGIN      
        1 gtttgatacc acagccgttt accttggaaa atttttgcta tttgttcgaa aatcttccaa
       61 ttatcaaaat tacgagaaat acttttttta ttgctacaat tataacggtt gttaagttag
      121 gcgtagcttt ttttgcggca tatagtttta cttattgccg aatcaaaggg aagaaaatct
      181 ggtactttat catcattggt acgatgttca ttccttttac tgttgcgatg attcctaatt
      241 atttgttgat tgcaaaactt aatttaattg atactacttg gggtgtgata ataccacaat
      301 ttgctgatgc gatgggcatt tttttattga cgcaaacaat gcgggaaatt ccagtgagcc
      361 ttctagatgt ggcggcattg gataacatca aacaaacaac cgtcatgagt aaaattgttt
      421 ttcctcttac tagacatgcg attacttcta ccggtatttg gttttttatt acctcttgga
      481 atgagtatgt ttggccggta ttaattttac ggacagtaga taattatact ttgccgttgg
      541 cgatgcagac ctatattagt tctgaaggtg ggacaaattt taccgtagca atggcgatta
      601 gtttaattac aatgtttgta ccgttagtct tgtatgcaat ttttcaaaaa tatattatcg
      661 ggacttttgt ttctgcaggt attaaataaa ttggaggaca aatcatggga gaaacgcttg
      721 aattaagaaa tatcggaaaa aaatatggag aacataccat tttaaaagaa attgatttag
      781 ctgtggccgc tggagaacga attgtgttac tgggaccatc tggttcagga aaatctactt
      841 tgctgagaat gattgctggt ttggaaaaaa ttacgagtgg agagcttttt ttaggtacac
      901 aactagcaaa taagctacct tgtggagaac gagagatcgc aatggttttt caaaattatg
      961 ctttatatcc tcacatgact gtaaaggaga atattatttt tgcattaagg gcgaataaaa
     1021 ttaagacagc tgacattcag caccgtttgg aagaagcttt gaagatgtta ggtttaacgc
     1081 cataccaaga tcgacttcct aaagatttgt caggagggca acggcagcgt gtagcattgg
     1141 ctcgtgcggt ggttaaacgg agtaattatt ttttattaga tgaaccgtta tcaaatttgg
     1201 atgtgcgttt acgattggat gcacgaaaag aattggtaaa aattcatcaa cgttataaac
     1261 aaacttttgt ctacgtcact catgatcaaa ttgaagcgat gacgttagct gaccggattg
     1321 ttttattgaa tgaaggtaga attcagatga ttgatacgcc gaaaaatgtt tatacgaaac
     1381 ctaaaaatac ttttactgca aattttattg gttcgccagg catgaatatt atgccagtta
     1441 cttatgatca aggaacatta aaggtagcca gtcaattttt aacattgaat ccagcttggc
     1501 aaaaacaact tcttcaggtt agtggctcat tgtatttagg cataagacca gaacacgtaa
     1561 ccttttcaaa agaaacaagc gatttacacg gtcaagtgaa gtatcgtgag ctattaggtc
     1621 aaagttatgc gtacaccgtt aaagtcgatg gtatagaaat gattgtcctt aatgaaaaac
     1681 ctacttggca gattggtgat gcagtcaatc tccgcataga acctgaaaga atgcattttt
     1741 ttgattatca gacgcaagaa aatttaggat atcctgatta ttttattgaa gaaagaggga
     1801 atagccatgc gctttttgca tctctctgat atccatttta gagaaaacta tcaaactaac
     1861 ttgtttactg ctgaactatt tccaacaatt aaaaatccac agttggcatt ggaaaaattg
     1921 ttacaagaac tctcgttaac agtgtacgat tttattctat taacaggtga tttagtacat
     1981 gaagggggga tttctgatta tcaagtctta cggaaaatgt tggatcaata tttcccaacc
     2041 acaccttact atttttgtcg tgggaatcat gatcgtcgca gtgttttctt tgaaggaatg
     2101 agtgttgaaa gcaatgaaac gggagagtat atccattgta cgaatcaaaa tgggttacga
     2161 attatttcct tggattctgc tcaagatacc cagcacgaag gaaaaatatc gataggacag
     2221 atgattcaat tacaagagtg gttgggtaca cctgctgaaa agggaacgat tttattggtt
     2281 catcatccat tgtcgtggga agatccatct attgtaactg aagttccgga aggttttgct
     2341 gaattgattg ccgaaagtga tattttaggt atttttgtag gacatatcca tcaaggtagc
     2401 agtgctgaat ttgctggtaa aaaacagttt atggctgaat ctttatcatt tggtgtagat
     2461 gaatacccag actattctct ttttaccaat agaacaggat tcactagtgg cagtttaatt
     2521 gacggaaaaa tttttactta tcagcattat ttaactccac atcaaataga aatagggcgc
     2581 atgaaaaaac caattactgg taatcattaa aaattattga ttaaaaataa agacttcatt
     2641 cactttttag ccgtggtgct caatgtctgg ttccacttga agaacttgga aaatcgctat
     2701 caataaacca ctatccctcc aattacaaaa agggtggaga aagttttcca agaaccatat
     2761 cggtcttcgt ggttgtgcca ttcgtttttt accagagcat accacatctt tgtggagttt
     2821 tcagacacgt cctagttaat ttggttcttt taggagtctt ttcctgttct gtgtgtcgtt
     2881 gagtgttcgc ttcaattatc gctgtggcgg attttgaaac ttttctatct atcaaggcca
     2941 tttagcacta tatcctttcc ttatatttag ataaatacac tgcatttcaa ggagttcaaa
     3001 agtagttgta ttaattccat ttgaactctt tccgtgatat aatgtgtttg tcagaaagag
     3061 gtggagaaaa tggctaagtt acaaatttct atggacgaaa aattgaaaaa tgaagcagaa
     3121 agcattattg atgaactagg catgagcccc aaaacagccg tcaccgtttt ttataagcaa
     3181 attgttgagc aaggaaaaat cccattttct attgaattgt cggaaaaaag taaacgaatc
     3241 cataatattc aaagattagc ggaaaaattg cctgtccaaa aactagacac tgacgaaaaa
     3301 attgaagaat ggttcaatga agatgaatac taatgatata gcaaccgtct acgttgcttt
     3361 cacagctgca gagggcggta aacgccggcc tgtctatatt ctaaaaaatg gactggacac
     3421 aatttcgttt ctgtcaatta cttcaaaata tcatactaaa tccgataaaa taaagctgaa
     3481 atacgttgaa ataagggatt ggaaacaggc aggactaaaa aaagaatctt ggattgacgt
     3541 aggaactcta aacgaattac ccaaaaacaa caaaaaggtc aaatggaaaa aaattgggga
     3601 attaagtcca agcgatttac agcgactgga agaacgcatg gaagaattat aataatacta
     3661 aaaagaaggg ctctttgtca ataaggactg atgggttgta caaaatcaaa tctgggtcag
     3721 aatgaacctc attctggctc agatttttgg ctctttgtca ataaggactg atgagttgtg
     3781 caaaatcaaa tccgggtcag aatgaaccac attctggttc agattttttg ttatcttaca
     3841 ttgattaatt gattgatcaa aaagattcta attctcatgt tctcgaatga tttaaatccg
     3901 taggatactc gtttcattgt ctttatgtgg gtattcttcg cttcaatttt tccattggaa
     3961 taaggataga tcattgcgct ggtgatacct tcttcatagg tcagaaggtt ttgaagcttc
     4021 tcccgaaagc cgtcatctaa cgtttcggga agttctgcca ataaggagaa aaataagtca
     4081 gggtctttgt ctcgaaaggc ttcgactaat tcatgaaaaa agggatacgc ctcctttaga
     4141 ggcgcagaaa actcaagcaa tcgatcaatc atcattgctt cagtgagaaa tgggtatttt
     4201 ggtgctcgga aacttttcca tgttttgtac tcataatggt tgatgcttgc acgatttttt
     4261 agcagaaagc gccagttctt tttcagtttt tctgcctgac ttttctgtcc tgctttacga
     4321 agctcattca tttcacggat acgcaactca ttgagtgcct gattcatgtg tttgacaata
     4381 tgaaaccgat caatcactac tttcgcattt ggcagaacac gtttggtaag ctggaagtaa
     4441 gcggcgttca tgtctgtgac taagaattct acttcttctg gattggtgca gcctaagaaa
     4501 tagcttgtta atcgaggtaa tttacgcgta ggcaagacag caattaagtt tcctgtttca
     4561 ccatctgcgc aaataaagct cattttatct tctatggaag cgtgcgaacg aaattcatca
     4621 accatcaata ctctggggag aatcttctta gaaggctttg gtaaatagct tttaaactct
     4681 ttcaatgtac gagtaacggt agtcaaagat acctgacaac ttttcgcaat aaaagacaaa
     4741 gatacttttt cagtcagtaa agaagcaatt ttatatcgaa catgatctgc gattgaatgg
     4801 ctgagtcgga caaaataact ttgagccgtc caatgggtac gacagttttt acaggtatag
     4861 cgttgctttt ttaggcgcgt aaccaaaggc atatgattgt attgttcaaa acggacaatc
     4921 gtttccttct tcccattttt cactataatt gctttcccgt ttccatctac cacagtagaa
     4981 ccacaacttc tacaagcacg aggagtaggc gagagaacag catcgacgac caacgtcttt
     5041 ttcttggctc tttgtcaaat aggactgata gagtgatata agaacatcta aggtagaatt
     5101 gattttgctt ctataatatt ttgtgttggt ggaaaatcga aggcgatttt ctactaacac
     5161 tttttcttcg tttcaactca aaaagagact cacttttggt aaaatttagt cgaccaaaac
     5221 gtcatcctac caaaggagtc tctcaatgga taatcatact agaaaattac tcggattaac
     5281 agataaacat
//
LOCUS       DACESX010000153         5155 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.155, whole genome shotgun sequence.
ACCESSION   DACESX010000153 DACESX010000000
VERSION     DACESX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.155"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(32..979)
                     /locus_tag="IXF88_002452"
     CDS             complement(32..979)
                     /locus_tag="IXF88_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1509158.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1157..1960)
                     /locus_tag="IXF88_002453"
     CDS             complement(1157..1960)
                     /locus_tag="IXF88_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1509159.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2106..3020
                     /locus_tag="IXF88_002454"
     CDS             2106..3020
                     /locus_tag="IXF88_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1509160.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3068..3538
                     /locus_tag="IXF88_002455"
     CDS             3068..3538
                     /locus_tag="IXF88_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ1509161.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3623..4231
                     /locus_tag="IXF88_002456"
     CDS             3623..4231
                     /locus_tag="IXF88_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ1509162.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4365..4565
                     /locus_tag="IXF88_002457"
     CDS             4365..4565
                     /locus_tag="IXF88_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1509163.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1699 a          789 c         1013 g         1654 t
ORIGIN      
        1 ttttttcttt tgaaaatatt gttttcagct attaatatcc tgcagacaat tttacttgat
       61 tggtttgaag ctcatgggtg gcaaagtagc ttttcagatt ggttaaaaaa atagccatta
      121 atttcttttg aaaatcaggt gtcaaacctg caatgtggga agtaatcagt acattgtcca
      181 ttttccaaag aggatgatct tgtggaagag gctcttcctc aaaaacgtct aatgctgcaa
      241 aggaaagttg ctggttattt aaagcctgca ccagatcttg tgtcttaaca gaagcaccac
      301 ggccaacatt gatgaataca gattttggat caaagtgttc aaacatatgc tgatcaaaga
      361 gcccagttgt ttcctccgtt agaggtaaga tattgactac gaaatctact tcaggaagaa
      421 cttcctttag ttgatttaaa gaaaaagtct tttccattcc ttctgctgga tgaccggatg
      481 tattgattcc atagcagttc atgtctaatg ctttgcattt ttttgcaatc atttttccaa
      541 tatggccagt tccaatgatt agtatttttt tcccttggat agattggtaa tggaccgatg
      601 aacctaacca tttcatgtca ctctgtgcct tttgtgcttg aaaaagacca cgagaatacc
      661 caaggatgat tcctaagaca tgctccgtaa tcgattcact gtggatgcca ctaccatttg
      721 aaagtaaaat atgtttcttt gaaaaagtct caaggggtag ctgatcaaca ccagctgaga
      781 tagattggac ccatttcaag gtagaagagg ctaatagttt ttcttggtag tctgagttcc
      841 agcccaaact gatctctaca ttttgtagat cctctggttg taattctgtt ttaaagatat
      901 aaccaggcgc tatagtatgg atttcttcga taaattcatc acgaaatgga cgatctgcat
      961 aaatgattct ttctgacata gttaatttcc cgcctttctt tactctaagt gtagcgcata
     1021 caagaagaac tttgaagcgc cttgcttgtt ttttgagaaa gaaatatttt tcaaataaaa
     1081 aaggggctgt gacaaaaaaa tgtcacaacc tctaaaaggg tctattgaag tagaaataac
     1141 gagttgtctt tatttcttaa ttgatcgata tttttgccca tcctttgaca gcttgagcaa
     1201 aaataaagat tcctgcaaaa gcaatgaagg cgctagtgtc aaaagaagga agaaagacca
     1261 tcaagattgt tgcaattata gtaggcaatg aaattgaata gatcaatgtt tttaaacaat
     1321 caagaaaagt agcttttgtt agtcgaaagc gtgcatagag aaaagcagca atgttggtaa
     1381 atagcaaagt gatcagcaga ttcagaaaac taggataaat agaaaatata aatgtcagga
     1441 cttttatcca aagtggtata gatgcttcac ttaaaaaaga acggagctgc ttacctgtta
     1501 agttttttaa agattcattg gtgtagggaa gttctaattg gttattgttc aacaaagcag
     1561 aagtcgtgcc tgtattagga agagctatga taagtttgtt ttttaacatc ccgacactaa
     1621 caaaattccc catcaaatca gtagagatgt cctgttctgt tcgttttccc tctggatcaa
     1681 atgtgaagat aatggagttt gtttggtaaa tgaatccttc tttttcttcg gtttgaagct
     1741 gcccgttttg gattgtaaaa tcaggaatgc gagtagcaat cttttgccca tccaattgaa
     1801 tgttgtttaa gacttgaaaa acttgaaaag agattggcaa agccataatt atactcaaaa
     1861 ctaaaagata gacaatggct ttccaaaatg ggacatttct cgcctccttt aggtctttga
     1921 atcgtataaa ggaactgata attaattgtt ttgttgtcat aagtactcct catctagtag
     1981 tgtcatcact tgcaattcta gcgaagtatc aagtgaaata caagtgcttt aaggaaacag
     2041 ctgaatgatt ggtgaaatat caaaaaaatg ttattcttgt ctaaagacta tccaatataa
     2101 ataaaatgga ggaaggaata gtgcctacac agccacagag atcatcaaaa aaactatttg
     2161 gagtagtggt tttgaatgcg gtgatcactt tatcagaatt tattggaggg attatgtcag
     2221 ggagtttgtc tcttgtttca gatgctttcc ataaccttag tgatacattg gcaattattt
     2281 tttcttacgg ggcacaaaaa atggcccgaa aagaagcaaa taaaaaacgt acgtatggct
     2341 accagcgttt agaaatattg agtgctttca tcaactcatt catattgatc atattatcct
     2401 tgtttttaac cgttgaagca ttcaagcggt tcacttcacc agaaaagata aatagtcatt
     2461 tgatgctaac agtagcagtt attggattgc tcgcgaattt gttctcagta ttattattac
     2521 gacaagaagc agatgaaagt ttgaatataa aatctagtta tttgcatttg ttaagtgata
     2581 cactgtcttc ggtaagtgtg attatcggag caattttgat ccgttttttc gatatctact
     2641 ggatcgatcc agttattaca ctgatcattt cgatatatat attgatagaa gcgataatgg
     2701 tgatcaaaaa agctgccgct attttgatac aaagtgcacc tgctatcgac tatgaaaaaa
     2761 tggaacaaga aattgaagca atcgatgggg taaaggatgt tcatcatgtc catatttggc
     2821 agtatagcga aaagataatt attttcgacg gacatattga ttttgaagat cagctactaa
     2881 gcgagattga aaagatatat cctcgtataa tttctttatt aaaattaaaa tatggtatta
     2941 cacatgtgac gatccaagca gagacaaagg caaaggatca aaaaaaatta atttttttaa
     3001 ataaagatga aagttattga aatatagagt atgataaata agaattttta ataaggtagg
     3061 ttggctcatg aagcagtatc gacagttaaa aggatattta gaagcaaaag gatattggga
     3121 gatactagtc tatctctttt ttggtggttt ggctacgatt gtaaattttg ttagttttgc
     3181 tctcgcacgc caatattttg atttaagtat ggccttatcc aattctattt cgtggttttg
     3241 ttctgtattg tttgcttttg taacgaacaa actttgggta ttccattcaa aatcaccgaa
     3301 ttttacacat gcacttattg aatgcggtaa gttcttcttc taccgtatcc tttcttacgg
     3361 attggacatg ggggcaatgg tattgttgat caatgttatg aacagcaatg agtatgtggc
     3421 gaaaatcatc actcaaatta ttgttatcct tgcgaactat attttcagca agttgtttat
     3481 ttttaaagaa actgaggttt ttgaagaaga agtaggtcaa agtgaaaaag acgactaggc
     3541 ataacaattg aagaatttcc ttctctttgt tactctttgt aatgtagaca ttattaagaa
     3601 aacattgagg aggaattttt ttatgactta cacattacca gatctacctt atgcatatga
     3661 cgcattggaa ccttatattg acgaagaaac gatgcatctg catcatgata agcatcacaa
     3721 tacttatgtg acgaatttaa attcagcaat tgagaaatac ccagaattag gcgaaaaaac
     3781 aatagaagaa ttattatctg atatggacgc tattccaaca gatatcaaga cagctgtacg
     3841 taacaatggt ggcggacatg ctaaccattc atttttctgg gaaatcatgg caccaaatgc
     3901 tggtggcgaa cctacaggag aaataaaaga agcgattaat gaagcttttg gtgatttttc
     3961 ttcttttaaa gaagaattca aaaaagcagc cgctggacga tttggttctg gatgggcttg
     4021 gcttgtaatg gaaaatggaa aattagctat tacctctact gcaaatcaag attctccatt
     4081 gatggaagga aagacaccaa ttctaggttt ggacgtttgg gaacatgctt attatttgaa
     4141 atataaaaat gtccgaccag attatattgc agcattctgg aatgttatta attgggatga
     4201 agtaaacaaa cgtttcgaag cagcaaaata accatttcct ccgtcatatc agtcttaaaa
     4261 ataatttgga aaataaaaga gcgaaagctt gctagcgttt tctaaattgg gtataatgtt
     4321 cttgcaaggt tacttgcaag aacttttttg gaggatatac atttatggaa cacggaacgg
     4381 ttaaatggtt caacaatgaa aaaggatttg ggtttattac agtagacggc ggagatgacg
     4441 tatttgttca tttctcagca attcaaggag acggcttcaa gagcctagaa gaaggtcaag
     4501 aagtagaatt cacgattgtt gaaggtgcac gcggacctca agctgctgaa gtgactaaat
     4561 tataataatt tgttgaataa agacacgact tcttagagag tcgtgtcttt ttgtctattt
     4621 atgaagtttt tagatttttt ggctaagtaa atgcatttgt tcacttgaca tgtcctaact
     4681 aattatcaag caaggaacat tttcttcgtt agtaatggat tttaaagaaa tttcagtttt
     4741 tggatgttga ataaaagacc atttgtgaaa ccactagtat acatactcat taccatgtca
     4801 attctattac tttaaaagaa aatcgtttta ctttgaagag gagcatatct atccattaaa
     4861 agatgatggt aatgcaatca ctagttcgag tcattttaag agccatgcag tgagctggaa
     4921 ataaaacgaa tagaagatgg gtttaacaaa aggaccagtc ataggaagaa attgtcgtga
     4981 aatcgaatga taatctgaac ggtaggcaaa gactgttaaa aagatgggaa aggtatgtgc
     5041 gatcatttta ctgcacgatt atttggaaaa taccttgttg ttcgcttttt ctggaaaaaa
     5101 tacataaaaa aagataaaac gattgcgaac tgcgtgtatt cggtctttcg atctt
//
LOCUS       DACESX010000154         4902 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.156, whole genome shotgun sequence.
ACCESSION   DACESX010000154 DACESX010000000
VERSION     DACESX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.156"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="IXF88_002458"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="IXF88_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1509164.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="IXF88_002459"
     CDS             complement(2065..3063)
                     /locus_tag="IXF88_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1509165.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="IXF88_002460"
     CDS             complement(3289..3942)
                     /locus_tag="IXF88_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1509166.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="IXF88_002461"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="IXF88_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1509167.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1450 a         1021 c          753 g         1678 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctcccatct
     4741 gtttctatta tacataatca acttttaaat agctaccttt ttttcataat ttttttaaaa
     4801 aaatgaaagt tatctaaggt taatatacag taaaaccagc attctcgctt tttttatcat
     4861 ttaaaaatta gagttttcaa atctaattca aattttctaa ac
//
LOCUS       DACESX010000155         4847 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.157, whole genome shotgun sequence.
ACCESSION   DACESX010000155 DACESX010000000
VERSION     DACESX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.157"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            6..473
                     /locus_tag="IXF88_002462"
     CDS             6..473
                     /locus_tag="IXF88_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1509168.1"
                     /translation="MNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSAEE
                     IFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHLLC
                     LNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            569..1456
                     /gene="xerD"
                     /locus_tag="IXF88_002463"
     CDS             569..1456
                     /gene="xerD"
                     /locus_tag="IXF88_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1509169.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            1475..1846
                     /locus_tag="IXF88_002464"
     CDS             1475..1846
                     /locus_tag="IXF88_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1509170.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            1816..2613
                     /locus_tag="IXF88_002465"
     CDS             1816..2613
                     /locus_tag="IXF88_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1509171.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            2597..3166
                     /gene="scpB"
                     /locus_tag="IXF88_002466"
     CDS             2597..3166
                     /gene="scpB"
                     /locus_tag="IXF88_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1509172.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            3172..3888
                     /locus_tag="IXF88_002467"
     CDS             3172..3888
                     /locus_tag="IXF88_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1509173.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      3970..4095
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4218..4829
                     /locus_tag="IXF88_002468"
     CDS             4218..4829
                     /locus_tag="IXF88_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1509174.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
BASE COUNT         1630 a          841 c         1067 g         1309 t
ORIGIN      
        1 tggtcatgaa tgctatttcg gcgatgaaaa aaacaaaaca gcagctacat gaagcggggt
       61 ttaagctgac accgcaaaga gaagctactc tgcttgtgct tttggaaaat gagaaagagc
      121 atttatctgc agaagaaatc ttctttttag tgaaaaaaaa gagtccggaa attggacttg
      181 ctactgttta tcgaaccctt gaaatcttga cagaattgaa aattgtcgat aaagtaagtt
      241 ttaatgatgg attagcccgt tatgatttga gaaaagaagg cgccaaacat ttccatcatc
      301 atttactatg cttgaactgc ggcacgatcg aggaagtaga agaggatctt ttacttgaag
      361 tggagcaagt agtagaagaa cgttatcatt ttttagtaaa agatcatcgg ctaacgttcc
      421 atggaatctg tcaggaatgc tatgaaaaag aaaaaagaaa ccagtccgtc tgagtgtcta
      481 gacggctttt tctttgctca gattatggaa aaagactatc agaatgaagc ggtatctgat
      541 atgatagaga ggcatgaggg ggattattat gaaagagcaa atcaacgatt atctccacta
      601 cttgaatatt gaacggggat tgtccgccaa tacacgacaa agctatgaac gtgatttgga
      661 acagtacttg cattttctac aggagcagaa aattgcttca tggcaggatg tcgatcgctt
      721 tttagtcatc cgttttctgg agaagttgca tgaagaaaaa aaagcgacag caactgttac
      781 tcggatgatc tcaagtctca gaagattcca tcagttttta cggcaagagc gtatcactga
      841 tcatgatccg atgcaacata tcgatacgcc gaagaaagca cagaaattac caagtacatt
      901 gactctgaca gaagtagaaa agctgattga agcacctgat acgacaaaaa cgttagggat
      961 aagagatcgt gcaatcttgg aagtcatgta tgctactggg atgcgcgtca gtgaattgat
     1021 tggattgaaa ttgggagatc ttcatctttc tcttggactg gtacagacag ttggaaaagg
     1081 agataaagag cggatcattc ctttaggtga ttatgcgatc caatggctgg aacgctactt
     1141 agacgaagca cgtccattac ttgtaaagga cgcatctgaa atgcatgtat ttgtcaataa
     1201 tcatgggaaa ggtctttctc ggcaaggaat ctggaaaaat ctgaagcagt tagtacgaga
     1261 ggctgggatt tacaaagaag taacaccaca tacgctgcgg catagctttg ccacacatct
     1321 gctagaaaat ggagctgatt tgcggacagt acaggaattg ttaggtcacg cggatatctc
     1381 tacgactcag atttatacac atatcacgaa gaaaagaatg acggatgttt ataagcagca
     1441 ttttccaagg gcttagtcat aggaaggtga gaagatgtta acacattacc ggaaagaaaa
     1501 ccgtaaaatt gctatgggac tattgagttt ccataagaaa ctgacaggta aaacttcttt
     1561 gttgaaggag attgatacgt acgaatctga agacgattat gaactttttt tctttacgcc
     1621 agaaggttca accaacattc aagggattat tggtatttac tgggcaacga acgatcagct
     1681 gatcatccat gacatttctt tgaatccgtc ttatcgcggt gagaatatgg gctttcaaat
     1741 gttagatgaa ttacaggaaa agtacccgga tgtctccatc cgtgcaactg aaatcacaga
     1801 gccttacatt ggaaaatggc tagcaaagaa aaagaagaaa gagtgaatga gttgacggag
     1861 atcaatatta aattagatgt attcgagggt cctctcgatc tgctgcttca tctgattcag
     1921 caaatggaaa tcgatatata tgatataccg atcgctacga tcacggagca atatatgaat
     1981 tatatccata caatgaaaac actcgagcta gctgttgcag gagaatactt agtgatggct
     2041 gctacattga tggcaatcaa aagcaagaca ttgcttcctg tccaagaatt cgtcacagac
     2101 gatgaagaat tatgggaaga agatcctcga gaagaattgg tcactcagct tttagaatac
     2161 cgtaaatata aatatgcagc tgaaagactg acagaaaaag cagaggaacg aagtctttat
     2221 tacacaaaag aacctatgaa catcgatggg ttagcggatg ctgaaaagcc gttgaagcga
     2281 ggacaagtca aaaaaatcga tttgatccgt gccatgcaga aaatcttaga acgtaagaaa
     2341 ttttctcaaa aagtggaaaa aacgatcacg cccgatgaca caacgatcga agaacgaatg
     2401 gtatttatcg aagagcgtat aaaaagaaat gcgcatggct gtactttcga ttcttttttt
     2461 gagtcacctt ctaaaagtga agttgtcact acttttatgg ctttgctaga attgatgaaa
     2521 aaagggcaga tcattgcgca acaaaaagaa aactatgaat caattatgct ttatccagca
     2581 gtaggagata ccgaacatga cgaaattaag tgaacttgaa gcccttcttt ttattgttgg
     2641 cgaggaaggg atcagtaatg aagaattaag cggactgtta aatctcagcg aagcagaagt
     2701 cacaagttta gtgaaagaat taaaagaatc ttacactcaa aactcgcact cagctttgca
     2761 tatttttgaa acggaaagcc attatgtatt aacgactaaa aaagagctgg ctcccttact
     2821 taagcgatat gcgcaaggga catcaaacac attatctcaa gccgccgtcg aaaccttagc
     2881 aatcatcgct tataaacagc caatcacaag agctgaaatc gagcagattc gaggagttca
     2941 ggtttcagga gcggtacagc gtctagctgc ccataaactg attgaagaaa aaggtagagt
     3001 agaaggacca ggtcggccga tattatatgg aacaactggc tacttcttag attattttgg
     3061 tttgaaatca ttagaagaac tgcctgatac acaagaaatg gaaatttcac ttgaagaaga
     3121 cgtgtcacca gatctattct ttgatggaat gaataaagga gaataattgt aatggagaga
     3181 ttgcagaaag taattgccca cgctggcatc acttctcgtc gaaaagcaga agaattcatc
     3241 cttgcaggac gtgtaaaagt aaacggacaa gttgtaaggg aattagggat aaaagttggg
     3301 aaacatgaca ctgtggaagt ggatgaagtc ccaatttatc aagaagaata cggttactat
     3361 ttattgtaca aacctaaagg agttatttct gctgttgcag atgacaaagg aagaaaagtt
     3421 gttacggatc ttttgccgat ggtaaaagaa cgtatctatc ctgtaggtcg actggattac
     3481 gatacttcgg ggatcttgct actgacaaat gatggagatt ttgctcagcg cctgacacat
     3541 ccaaaacatg aagtggataa agtttacgtt gcaaaggtaa aagggatcgc tacaaaatcg
     3601 atgcttgcac ctttgacaaa aggaataaag atcgaaggac gaaaaacatc tccagctaaa
     3661 ttccaaattt tatcagtgga tcctaagaca aaccacagta tcgtggaatt gacgatccac
     3721 gaaggacgta atcatcaagt gaaaaatatg cttcaagcag ttggtcttcc tgtgcaaaaa
     3781 ctaaaacgtg aaaaatacgg agatctgact ttacaaggcc tacgtcctgg ggattaccgt
     3841 gaactgaaca aaaaagaagt cagcagtttg ctgaaccact caaaatgaac acttactttt
     3901 tataagtgtt agatgttgcg ttttttgaat agatatgtat aatgaatatg taaacaaaaa
     3961 ttatataagg ttcgtcttca ggggcagggt gaaattcccg accggtggtt atagtccacg
     4021 acctattttg caaaaaaata gctgaatcgg tgaaattccg ataccgacag tatagtctgg
     4081 ataaagaaga tagagcttat ttgacgacgc ttgatcaaat aactctaact ctattttccc
     4141 tggaaaatag agttttttca tttggagaga aacgtcaaaa agttcgctga agaggagtcc
     4201 taacaggagg atttcaaatg aagaacaccc gtgtacagaa aatggttgca gtagcgatgt
     4261 ttgctgcgat cgcattagtt ttacagtatt ttgcttttcc gatcatgccg gcttttagtt
     4321 ttttgaagat cgattttagt gatatctcgg tattgatcag catgttctta ttcggtccag
     4381 cggccggagt tgcaacagcg ttcttgcgtt cagtattaca tctgctgact acaggttttt
     4441 ctccggataa tttggtagga gacactgcta gtttcttatc aacaacgatc tttacattcc
     4501 cgatttatta tttctttagt gtaaagaaac agcataaagg aagaaacaaa tttttaggtg
     4561 tcattacagg aaccatcgct ctaactattt ttatgagtat cgcaaattat ttcgttatta
     4621 cgcctcttta cttaatgttt ttagggctga acgcaacaca gatgctgggc atgtctttag
     4681 caaactatgt actgattggg atcgttcctt tcaacttgat caaaggattc atcgtgagtg
     4741 ctgtcttttt agtgctgcat gcgcaacttc ttccatggtt aagtcgaaaa caacatcagt
     4801 tcgaacaacg tcatacagca gtcaaataaa tatcaaaaag atcaaga
//
LOCUS       DACESX010000156         4829 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.158, whole genome shotgun sequence.
ACCESSION   DACESX010000156 DACESX010000000
VERSION     DACESX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.158"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            184..1107
                     /gene="truB"
                     /locus_tag="IXF88_002469"
     CDS             184..1107
                     /gene="truB"
                     /locus_tag="IXF88_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1509175.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1111..2052
                     /gene="ribF"
                     /locus_tag="IXF88_002470"
     CDS             1111..2052
                     /gene="ribF"
                     /locus_tag="IXF88_002470"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1509176.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2447..3232
                     /locus_tag="IXF88_002471"
     CDS             2447..3232
                     /locus_tag="IXF88_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1509177.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3245..4096
                     /locus_tag="IXF88_002472"
     CDS             3245..4096
                     /locus_tag="IXF88_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1509178.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4220..4672)
                     /locus_tag="IXF88_002473"
     CDS             complement(4220..4672)
                     /locus_tag="IXF88_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1509179.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1613 a          795 c         1015 g         1406 t
ORIGIN      
        1 tcttccattc tattccctcc tttaaaaaaa acataacatg atttctattt acgtgagcat
       61 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
      121 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
      181 gacatggacg ggttattaac tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
      241 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
      301 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
      361 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
      421 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaagaaaaa
      481 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
      541 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
      601 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
      661 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
      721 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
      781 acccgaacag caagtgccgg tatgtcccag aaagatgctc tgacattaga agaagtagct
      841 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtgaaa
      901 cgattcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
      961 cttaattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     1021 aaaggcaaag ttgtcagtat ctatcagcca aacccgaaag aaaaaaacaa attaaaacct
     1081 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     1141 caatcagaac aaattccaga agacgatgtc gttttagtac taggattttt tgacggcgtg
     1201 catattggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     1261 aaattagcat tgatgacttt caatcagcat ccatcgattg tctttaaaaa gatcgatcct
     1321 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     1381 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     1441 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     1501 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     1561 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     1621 attcgaggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     1681 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     1741 acagcaaatg tcaaagtaaa gagcacagtt catttgccta aagaaggtgt ttatgtatcg
     1801 gaaataaaaa ttggggaaat ttggtatcca gccatgggtt caattggaca taatgataca
     1861 ttcggtgaag gacgggagct gactgtagaa ttgtatattt tggactttaa tcaagatatc
     1921 tatggtgaac atgtcgcagt acgctggaat caatttttaa gagatcaagt gaaattttct
     1981 agtgtagagg aattgattga acaactcaaa gaggatgaac aagcaacagc tgaatatttt
     2041 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caatttcaat
     2101 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     2161 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcacaa
     2221 aaatttgaga agtaattttt gtaaattact tcttatttct tgaagctggc cacttctgtc
     2281 acaacctctt tctttataga aataaaagga cactagcaat tcatgaaaac gattgctaaa
     2341 gcgtatcatt tgctttagac tatatatctt ggtgtacact cactttaaga cggaaataaa
     2401 agttatccgt aataacaggt ttaggtatga aaaaggagtg ggtactatgt ataaggaatt
     2461 agaaaacaaa gtcgcagtag taactggtgg ctcaaagggg attggaacag cgattgctca
     2521 acgattcggg aaagaaaaaa tgaatgtagt tgtcaactat aactcggata aagaaggagc
     2581 agaaaaagca gtcaaagcaa tcgaagaggc aggcggaaaa gcagtctctg tacaagcaga
     2641 tgtcagttct gaagaaggaa tacagcgttt acttgatacg gctttagaaa attttgggac
     2701 actggacgtt tggattaata atgctgggat ggaaaatcag catccaaccc atgaacttag
     2761 ccttgaagat tgggaaagag tattaaaagt caatttgact ggcgttttta tcggaacgaa
     2821 agcggcttta aattacttta cagagaatga taaaaaagga aatatcatca atatgtcttc
     2881 tgttcatgaa caaatcccat ggccaacgtt tgctcattac gctgcttcaa aaggcggagt
     2941 aaaactattc accgaaacgg tagcaatgga gtacgcacca aagggaatca gaatcaatag
     3001 tataggacct ggtgctatcc gtacacctat taatgcggca aaatttgatg atcctgaaca
     3061 aaaagaactc ttggaaagta tgatcccact aggacgcgta ggtgaaccaa aagaaatagc
     3121 ggcgacagct gcatggttgg cttcagacga atccagttat gttacaggta ttacactatt
     3181 tgcagatggt gggatgactt tatatccttc tttccaaggt ggaaaaggat aagggtgaat
     3241 aaaaatgggt ttattgattg cattgatccc ggcagttggg tgggggatcc agccactgat
     3301 tttgaaaaag attggtggca gaccaacaaa tgagatatta ggaactggta ttggagccgt
     3361 gctcgttggc ttacttgttc aattattcgc ttctccagct ggtatttcag tgactacatt
     3421 tttgattagt ttactttctg gtgcattctg ggtcatcgga caaacaggac agtatacatc
     3481 tttcaacttg attggtgttt ctaaaacgat gccaatttcg actgcattac agttagtcgg
     3541 aacctcattg attggtgtct tcgcttttgg agaatggtct ggaagtatgg gaaaaatcat
     3601 tggtgcagca gcaatcattc ttcttgtatt tggttctgct ttaacggcaa tcagtgatga
     3661 tcctggaaaa aaaggcggta tcacaaaagg aatcagtcta ttagcatcaa caagtattgg
     3721 ttattgggtt tacagtgcat tgcccaaact agttgatgca agcggacttt ccattttctt
     3781 tccgcaaatg atcggtgtct ttttaggggc agtgatttat gtgattttcc gaaaaccaac
     3841 cgcattttct gaagacaaga gttggaaagc aacaatagtt ggagccatct tcagtgtgtc
     3901 agcattggct tatatttttt cagcaaatga aaatggggtt gctactgcat atatcattac
     3961 acagctaaac gtagttatct ctacattagg cggtatgtac atcctgcatg aaaataagac
     4021 accaaaagaa aaaaaattaa cgattatcgg attattgctg atcgtagtag gaagcatggt
     4081 cacggtcttt atttgaggat aaagatggta aaatctcatt tattaaaaaa atatttgttt
     4141 gaaccaaaca gtaagtactt ataaaaaaat agcgctagaa tttccaacat ctctggaatt
     4201 tctagcgctt tttaattgtt taagatacaa gtgaaatatc tgtctgcatc aataattttt
     4261 cattgatcca ttgactaaaa tttacgacat gattgaagtt accaccgatt agtatctccg
     4321 catgattgtc tttataaaga tcgtattgta aaagacggtc ttttttattt gcgtaaacat
     4381 cgttatggta ctggactaat tgttctcgga tttgattgat tgctgtaaaa tcacgatact
     4441 gaaggttgaa acgcatcgtg attggtgcat ttagcatgaa tcgctgcatt tcttttgctg
     4501 cttcctctgc ttgatctccg tataggaact caaaaagatt acgttcagaa aatttccatt
     4561 gcccgccaac ctttgttcct tttagttttc cttcatgaag ataatttctg atagaacgtg
     4621 tagtaacacc taattgttcg gctaattcat ttactgagta aaaatctttc aatgttcacc
     4681 ctcctatgtg aatggttcta ctgacttagt tgatatttgc ttaatcacga aatgtagaaa
     4741 gaacgatttt aattaaggtc attgtaaaaa tcaagtcaga aaattctaac ttttctttct
     4801 ctaaagaaat tcgttctctc ctaaagtga
//
LOCUS       DACESX010000157         4819 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.159, whole genome shotgun sequence.
ACCESSION   DACESX010000157 DACESX010000000
VERSION     DACESX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.159"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(45..1019)
                     /gene="lacD"
                     /locus_tag="IXF88_002474"
                     /pseudo
     CDS             complement(45..1019)
                     /gene="lacD"
                     /locus_tag="IXF88_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
     gene            complement(1016..1474)
                     /locus_tag="IXF88_002475"
     CDS             complement(1016..1474)
                     /locus_tag="IXF88_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1509180.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(1471..2934)
                     /locus_tag="IXF88_002476"
     CDS             complement(1471..2934)
                     /locus_tag="IXF88_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1509181.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(2916..3866)
                     /gene="pfkB"
                     /locus_tag="IXF88_002477"
     CDS             complement(2916..3866)
                     /gene="pfkB"
                     /locus_tag="IXF88_002477"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1509182.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            4009..4757
                     /locus_tag="IXF88_002478"
                     /pseudo
     CDS             4009..4757
                     /locus_tag="IXF88_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
BASE COUNT         1427 a         1087 c          787 g         1518 t
ORIGIN      
        1 tatctaattg cagcagcatc tttttatgaa gaaacaactt tttcttatct atcaacgtag
       61 acaggtgttg ctgtttcttt taatagtgta cttaattcct taatattttg ttttccttct
      121 tctaacatcc aactactcgc tttttcttcg ccttcggtaa caaaaggtgc cactcccttc
      181 gcccatgttg ctcgcccaca taagacacca ttgaatgtcg aacctgattg ttttgcaaaa
      241 cgtaatgtat cttgaaataa cgtagcattg actcccgcac ttaaaaaaat aaacggttgt
      301 tcagtcactt tcgattgttc atagaaatac gcggatgcct ctgcttgact ataacaagtc
      361 tattgcccat atccttccac aaatctcata ttcactggca cttctacttt caatacatcc
      421 acttgataac gttcttttga aaattctcgc atggcttcaa taactttatg gggttttact
      481 tttgcatagg caagagagga cggatcgcca atagttgcat cataagaaag gatctctaaa
      541 aagaaaggta tgccttctgc atcgcattct gaaccaatcc gttctataac tatttgtttt
      601 tgcacattaa tctctgctgg ttcatccacg tcatagtaca ataaaaattt acaagcatcc
      661 gcaccagctt cttttaacct tttcaccgac caaatattca ataaatcagg taatcttccc
      721 ggcattttcg tatcatagcc ggttttttca taagctagca gcaagccaca ttctttctct
      781 ctaagcgctg ctgcaggcag gccgcattct ggatcaagta aaatagctga ggcataggga
      841 gtcaggtatt gagaaaccat ctgtttaaat tggatgatgt cttctgatgt agcttcttta
      901 tattggttga tcattttctt caaagcacca cgctggtcga ttgctaacgc gctaataatt
      961 ccagcttcat ccgtcaatcg cttcaaacgt tcctgctttt ctatactcat cttcttcata
     1021 gatcttcacc tatttcttta gttaatattg tggctatttc tgtttttgat tttgcttttc
     1081 tcaactgttc tgtaacctct tttcgcatca atgatcgtgc aacgagagac aataatttca
     1141 aatgagtcgt tcctgcttct gaatctggaa taaataacgc aaaaataaaa tcaactgact
     1201 gtctatctaa actgttccat tcaattcctt tgttcagtgt gacgataatc atactaggtt
     1261 cgttgattgt ttgagacttc gcatgaggga tagcaaaacc tgacattatt cctgtagtac
     1321 cttctttttc tctttctaga agttttcgat atacttcttt ttcgtcatga gcgattccta
     1381 aacagaatgt ttgctttgct aaaaacttaa atgcttcttc ttgactatct aatggctgtt
     1441 gtaaaaaaat gttctcttca tgaatgattg tcatgataag cctcctgctc ttccttcttc
     1501 gataactgta cgatttgctg ctttattatc catcttcttg tctttatcag ttactctttt
     1561 cttttggatc aaacctagta tcactccacc aacgatcgaa ccaataagaa tcgctgtaac
     1621 ccattggatt tttcccgtca ctacaggtaa cactaaaaaa ccgccatgtg gggctggtac
     1681 ttggacacca aacagatagg tcaagacagc agcaattgat gagcctaaca tcatcgttgg
     1741 taatactcgg atcgggtctt tcgcagcaaa cggaatagct ccctccgtaa tatgagttga
     1801 tcctaaaata aaattaacca aacctgcatt acgatcatta gtatcaaaat atttaccaaa
     1861 caataaagtt gcaaaaccag taatcaacgg tggtgcaatg caagctactg acacgccagc
     1921 cataaaagtc gtgtttcctt gtgccaaaag agctgtacct gttacatatg ccgccttatt
     1981 aatcggtcct cccatatcaa acgcacacat gcaaccaaca atcacgccaa gaacaagtgg
     2041 attcgaatgt tcaaatccag ctaaaaagct catcattcct tcatttatag ccttcattgg
     2101 acccgccagc aaagacatca cgatgccgat aaacactaca ccaataactg gatataagaa
     2161 gattgccttc aagccatcta gttgtttagg taaaattgtc agtagctttt gcaaaaataa
     2221 gataaaataa cctgctaaga acccagctaa aatacctact aaaaatccag taccgccact
     2281 catggccacg attccaccaa caaatccaac gactaaccct ggacgtttcg cgatcccttc
     2341 tgcaatataa gctgctaaaa ctggaaccat catccccatt gctgcatcgc caaccgtttt
     2401 taacatcgcc gctgtagcat tgtattgtgc atggtctgga tcaaaagaat agattcccca
     2461 aacggcaaat gaaatagcaa tcaatactcc gccagctacc acgaaaggca acatatgaga
     2521 gacgccattc attaaatttt tatagatgct gcgaccaaaa ttagcaccac ttatcgtttc
     2581 tgattctaaa gtatcaacag cttcattatc tgctaagatg gtcccttttc ctgccaatgc
     2641 ttcgtcaatc agccgttgtg catccttgat tcctgtactg acagaaacat caatgactcg
     2701 ttttcccgca aatctttctg aatggacatc tttgtctgcg gcaataatga cagcatctgc
     2761 ctgtttgatt tcttcagccg ttaattcatt ctcgatccca atttgtccat gggtctccac
     2821 tttgatcgtg atgccttttt ttctagctgc ttgctctaat gcctcttgcg ccatgtaggt
     2881 gtgtgcaatt cctgtcggac aaccggttgc tgcaattagt tgatattttc ccatctttcg
     2941 aacctctctt ccttgatttt gatttgttta cttaattcct ccacatccaa aaaatcagtc
     3001 agtcctttac gaaaagcagt agagctgcct gctgctatgc ttttacgtaa cgtttcatca
     3061 agcggtcgtc gattcatgta tcctactaaa aaagtgccta acatggcatc acctgcacat
     3121 gctgtattga caacttttcc ttttggcgag tttcctcgaa tcaccctgtc tttcgtaaaa
     3181 agaatcgctc ctttctcacc aagggataac aaaacatttt ctgcacctga ctctaccagt
     3241 cgttgtccat aaacaaagta atcttcatct gtaaccattt ttcgtccgaa ccaagaagaa
     3301 agttcttcct cattcggttt cagcaaaaat ggtcgatagg gcaaacaatc catgacctct
     3361 tgatcactgc tatcaataat caaatgaatc tccttttcct gacaaatccg actgatttcc
     3421 actaaaacct tcggagacat tcctcttggt aaactcccag aaacacaaag ataatcctct
     3481 gcttgtaatg ctcttatttg atttaaaaaa ttatggacag ctgcctgcgg tatttcaggt
     3541 ccttgattca ctaatttgta ttcttctctc gtttgattga cttgtgtaaa tacattgata
     3601 cgcgtcatac caggcacttc aataaactct gtttggattt gtttcttctg taaatagtct
     3661 tctatatatt ttcctgtgaa accagcagaa aaacctaatg ccgtacttgg gaaatctagc
     3721 atctttaata ctagtgacac atttactcct tttccgtttg cctgaatgtc atcctccttt
     3781 gtacggttta ccataaaagg atgcatctcc tctgtttcaa taaatagatc aattgccaaa
     3841 ttcatcgtgc atgtgtagat agacataacc aatgacctcc ttgaatctat tatgaaatac
     3901 gcgaaagaga aaatgttttc ttaatcgttc caacacaaag aaaaccttta catttataaa
     3961 cacatcgtgc tattttttaa ctaactttta gtgaaagaag gaaagaatat ggatcacaaa
     4021 ttaagtgaaa cagaacagta cctctggaac tttatcgaac accacatcct agagatccct
     4081 aactactcca tcgtgaaact aagtgaacaa gcaaatgtct caacagccac cattgtgcgg
     4141 acaatgaaga aaaaaggcta tgaaggattt acttcgttta agcaccatct aaaagaagga
     4201 gaaaacaaga acatcaattt ttctttttag ataaagtaga caaaggtatc cgtcgagcca
     4261 ttctaaaaaa tgaacaagaa gtggtacgta cgatcaatat gttggaaatc gggaacattg
     4321 aagatgcaat acaaagaatc aaatttactg accgggtttt tgtctttgcc cgaggatttt
     4381 cagaaatgat tggtcaagag atgctcgtaa aacttcaatt aacaggaaaa aattgtcaaa
     4441 tgcatactga tccagagatt atcaaaagca tcagtcggaa actaactaag aaagacagcg
     4501 ttatttttgt ttctttgaac ggcgagacaa aagaactcgt caccgccgca aagaactgct
     4561 atgatgcaga gattggttcg attctagtta cagccaatca ttcctctaca ctcaaagatt
     4621 attgcgagat aaatctcgta ggtttcaaat cagaaggttc ttatttccct gattatgaag
     4681 tacgttcacg cttgcctctc cagatcatcg cgagaatcct gcttgacgcc tatgtattgc
     4741 gtatgaggga gaattgagaa gcagtttatt atggttggtt acagtttaaa acatcacatt
     4801 ttttattctt tatattgta
//
LOCUS       DACESX010000158         4816 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.160, whole genome shotgun sequence.
ACCESSION   DACESX010000158 DACESX010000000
VERSION     DACESX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.160"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..795
                     /locus_tag="IXF88_002479"
     CDS             <1..795
                     /locus_tag="IXF88_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HAQ1509183.1"
                     /translation="KINIYQLVTKKEKKLFLNTTFENLTMINPNIMNFEDESIIEASG
                     FRIQPEYELFKRLFDIIVSLILLILASPFMLITAILVKTTSPGPIIYKQVRITKNQKE
                     FSIYKFRSMSATAEAKSGPVLAKSNDARVTPVGKFIRAVRFDELPQIFNVLKGDMSIV
                     GPRPERPFFVDQFNEENPYYYLRHNVRAGITGYAQVYGKYVSDYNSKLRFDLLYIKKY
                     SLLLDLKILLQTIKILFDKMSSQGLDEDENISSLAEIDFSQEKIYR"
     gene            854..1657
                     /locus_tag="IXF88_002480"
     CDS             854..1657
                     /locus_tag="IXF88_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1509184.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            1690..2544
                     /locus_tag="IXF88_002481"
     CDS             1690..2544
                     /locus_tag="IXF88_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HAQ1509185.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            2541..3242
                     /locus_tag="IXF88_002482"
     CDS             2541..3242
                     /locus_tag="IXF88_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAQ1509186.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            3250..4317
                     /locus_tag="IXF88_002483"
     CDS             3250..4317
                     /locus_tag="IXF88_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1509187.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            4310..>4816
                     /locus_tag="IXF88_002484"
     CDS             4310..>4816
                     /locus_tag="IXF88_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1509188.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKD"
BASE COUNT         1732 a          718 c          932 g         1434 t
ORIGIN      
        1 aaaatcaaca tttatcaatt agtgactaaa aaagagaaga aattattttt aaatacgact
       61 tttgagaatc tgacaatgat aaatccgaat attatgaatt ttgaagatga aagcatcatt
      121 gaagcttctg ggttcagaat ccaaccagaa tacgaactat tcaagcgtct ctttgatatc
      181 atcgtttcgc ttattttact gatcctcgcc tcacctttta tgctaatcac agcgattttg
      241 gtaaaaacaa cttcacctgg accaatcatc tataaacaag tacgtattac gaaaaatcaa
      301 aaggaatttt caatctataa attccgttcc atgagtgcga cagctgaagc aaaatcagga
      361 ccagtcttgg ctaaaagtaa cgatgcacgt gtgacaccag taggtaaatt tatccgtgct
      421 gtacgttttg acgaattacc tcaaatcttt aatgtattga aaggcgatat gtcgattgtc
      481 ggtccaaggc cagaaagacc atttttcgtt gatcagttca atgaagaaaa tccttattat
      541 tacttaagac acaatgtacg tgcgggaatt accggatatg cacaagtata cggaaaatat
      601 gtatcagatt ataacagtaa actacgtttt gatttgttgt atatcaaaaa atattcatta
      661 ctattagatt tgaagatttt acttcaaaca atcaaaatct tatttgataa gatgtcatct
      721 caaggactag atgaagatga aaatattagt tcattagcag aaatcgactt ttcacaagag
      781 aaaatttatc gctagattgg aaaaattttg agcaaagagg taggagagaa cattggaaaa
      841 taaaaatgta agtatgctgc tttctttata tgataaagaa aagccagagt atttaaagga
      901 agcattaact agtatttttg agcaaacagt aatgccaaaa gaaattgtat tagtttatga
      961 cggccctata aatgaaaaac ttcaagtagt tgtcacttat ttccaacaaa aagatcctca
     1021 cttgtttaca gttgtaaaat tagaaaaaaa tcaaggattg gggattgctt tagcaattgg
     1081 attagaacat acgaaaaatc gcttagtagc cagaatggat acagatgata taatggaacc
     1141 ccaacgatta gaaaaacaat tagagatctt ccaaaaagat ccagaaatag cgattgttgg
     1201 gagtaatatt gaggaattta ctggcacgct tagcaatgtt atcggaaaaa gaattgtacc
     1261 tgaatataac aatgcaattc gtgtattttc taaaagaaga aatcccttta atcatatgac
     1321 cgttatgttt gataaaaaag ctattttgga agtgggaaat taccaaccat taagtgggtt
     1381 tgaagattat tatctttggg caagattatt aaaggcaggt tataaaggat ataatattca
     1441 agaaacatta gtctatgcgc gaactggcaa agatatgtat gcaagaagag gcggaatgaa
     1501 atatcttggt tctggtctta tgggtcgtta tcgtatctgg aaagacggtt tagggtcagt
     1561 aaaagatttt ataattgttt cttgcggaca tatttttatt agcttactac caaatcaatt
     1621 aagaggaaaa ttttatcaaa cgcaattaag aaaataggag gtttttcttt gtatagcact
     1681 gatccaaaaa tgaaaaaaat acaacagatt acgttgaaaa tgtctcaaga gattgttgat
     1741 ttttgtctaa aaaatcaact tctttgttat ttctgcggtg gtggtgccat cggtactgtg
     1801 agagaacaag ggtttattcc atgggatgat gatttagatt tctttatgcc tagagaggat
     1861 tatgaaaaat tttatcaact atgggttaat tcagaaaaaa gtaaaaaata tcccatacag
     1921 aaggcttcta aaaattataa tgatcataat tcttttacaa ctatccgcga tgcagatact
     1981 acatttatta aaacttatca aagagattta gatatagtcc atggaattac tattgatatt
     2041 ttcccgttag atgttgcccc tgtttcgaaa tttagccgta aaattcaaaa actatgggct
     2101 ttagtatatg ctttattttg ttctcaagtc gtccctgaaa aacatggtgg aataatggct
     2161 acaggtagta agatactctt atctattttc cgctcacctc gtacgagata taagatatgg
     2221 tcttttgctg aaaaacaaat gacaaaatat aataatgaac caacgggcta tgtgactgaa
     2281 ctttgtgtcg gacctaaata catgggaaat gtttatcctg caaaagactt tgaatctgct
     2341 gtgtgggtgc cattcgaaga tacagagatg ccagtaccag ttggttatga taattattta
     2401 agtcaagttt ttggtgacta tatgcagctt ccgcctgaaa aagatcaggt ttctcatcat
     2461 gaagcagttt atattgatcc agaacatcca tacacaatgt acaaaggaaa atattattta
     2521 actaaaggag cagaaaaaaa atgattacag ctattattat tgctggtggt gtgggtaaac
     2581 gaatgggaca agatatccca aaacagttca ttacaattga aggaaaacct attattatct
     2641 atactttaga atcatttcaa caccatcctc aagttgaccg gattttagtc gtttgcaaat
     2701 ctggttggga agaaacgatg tgggcgtata tcaaagaatt caatattagc aaagtcaaat
     2761 gggtcattac aggtggatca aagggacaag agtcgatcaa taatggtgtt cagtttttga
     2821 aagactattc tgaagatgat gatacgatca tcatccatga tggaatccgt ccactagtag
     2881 acgaactggt attgtcagat gtaatcgtta aatgtaaaga atttggtaac ggtgtaacct
     2941 ctctacctta caacgaacaa atttttatca agaaaacaga agaaacgacg gaacagtatg
     3001 tcgatcgtaa cacattgagg cgtgtctcaa ctccacaagc ttatcaatac ggaaaactac
     3061 gggcagcata tgaccgggca gtgaaagaag acatcggtat gactgattct tcttatacga
     3121 ataccatgat ggtagatttg ggagaaactt tatattttgc tgctggttct gataaaaata
     3181 tcaaattgac aacaacagat gatctagaat tgttcaaagc atatttaaaa atgaaagact
     3241 aggtgaaaga tgatgacgtt gattaaacaa ccaatgtacc aagaagattt ggcaaaagta
     3301 atcaatcata caccaaaaat aaaacagcta catggaaaat ccgttctgat gattggtgct
     3361 tctggaatga tcggctcttt tttgattgat acgttgatgg ctgctaatga acaactaggc
     3421 atcaaaatca aagtttatgc aatgggcaga aaccgtcaga agcttgaaaa aaggtttgcc
     3481 agttatcttg aattagatac tttcgaaatt gttgaaggtg acgtcacaga acccttacca
     3541 aaagaaattc aagctgacta tttgatacac ggtgcaagta acacacatcc taaggcttac
     3601 gcaactgatc caatcggtac gatcatgact aatcttgcag gtacagagca agtattgaaa
     3661 catgcagtag caacccaacc ggttagagta ttatttttat caacagtaga aatctatggc
     3721 gaaaatcgag gcgatataga aaaattcaca gaagattact gtggatacat cgattgtaat
     3781 acactgagag caggctaccc tgaaggaaaa agagtcagtg aatcattatg ccaagcctac
     3841 atcgcaaaac acgacctcga catcgtgatt ccaaggcttt gccgtacgtt tggtccgacg
     3901 atgcttttat ctgatacgaa agcatcctca caatttattt taaatgcagt cgaacaaaaa
     3961 gatattgttt taaaaagtga aggaaaccaa tatttttcgt atgtatatgt tggagatgct
     4021 gtttctgcta ttcttcattt actgcttgaa ggagaaaaag gagaggcgta caacgtttct
     4081 tcagaaaact ttgatttacg actaaaagaa ttagcccaac agctagctga tatcagcggc
     4141 aaacaagtaa tttttgaatt gccggatgag attgaacaaa aaggtttctc aaaagctagc
     4201 acagcagtac ttgaaaataa aaaaattaaa gagacaggtt ggaaaccaat ttttcctttg
     4261 aacgaatcat tggttcatac agtagaatta gttaaaggtg agaaatataa tgtgtgagat
     4321 tagtattatt gttcctgtgt ataaagttga aaaatattta agaaaatgtg tagattcgat
     4381 tttagcccaa acatttactg attttgaagt tatattagta gatgatggat ctcctgacaa
     4441 tagcgggaaa atctgtgatg agtatgctga aaaagataat cgagtacgtg tgatccataa
     4501 agaaaacggc ggattaagca gtgctagaaa tgcggggatt gatgttgcga gaggcaaata
     4561 cttaggcttt gttgacagtg atgattatat tgatgaagat atgtatgaaa ttttgtatga
     4621 gaatttgaaa attcatgatg ccgacattag ctctgttgaa ttgattcctt tttatggtga
     4681 tagatataag aaagctaata aagaaaagaa agtaattata ttaaataaaa aggaagcaat
     4741 aaaatctgtt ttagaaggaa cgcaatttta tgcatacgca tggaataaac tttatcgtaa
     4801 ggaacttttt aaagat
//
LOCUS       DACESX010000159         4793 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.161, whole genome shotgun sequence.
ACCESSION   DACESX010000159 DACESX010000000
VERSION     DACESX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.161"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(53..1201)
                     /gene="nagA"
                     /locus_tag="IXF88_002485"
     CDS             complement(53..1201)
                     /gene="nagA"
                     /locus_tag="IXF88_002485"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1509189.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1277..2113)
                     /locus_tag="IXF88_002486"
     CDS             complement(1277..2113)
                     /locus_tag="IXF88_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1509190.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2297..3514)
                     /locus_tag="IXF88_002487"
     CDS             complement(2297..3514)
                     /locus_tag="IXF88_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1509191.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3897..3980
                     /locus_tag="IXF88_002488"
     tRNA            3897..3980
                     /locus_tag="IXF88_002488"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3931..3933,aa:Leu,seq:gag)
     gene            complement(4220..>4793)
                     /locus_tag="IXF88_002489"
     CDS             complement(4220..>4793)
                     /locus_tag="IXF88_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509192.1"
                     /translation="ILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMG
                     QSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYL
                     SFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELM
                     KSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1455 a          999 c          774 g         1565 t
ORIGIN      
        1 aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta acttaaatta tattatgcgt
       61 ggaaacgttc cacgccatct agataagttg cttccaatgt catatcaggg ttcaatacga
      121 taaagtctgc atcgcgtcct tgagcgattt ttccacatgt atcatcgatt ttacagctga
      181 ttgctggaat cagactagcc atcatgactg cttgttctgg tgttgctaat ccccagtcta
      241 caacattttt aattgcttct ttcaatttca atatacttcc agctaagttg ccagattcta
      301 gacgtgctgt tccttctttt actactactg gaaattcgcc taaattgtaa tttccatcag
      361 gcatcccacc ggccatcata cagtctgtaa ttaatgcgac atgatcatgt cctgcttttt
      421 ccattaggat tcccgcagca tttggatgta catgatggcc atcacaaatc aattcagaaa
      481 agacatgttc taaagataat aacgcaccca ccatacctgg ttcgcgatga ttcaatccac
      541 gcataccatt gaaagcatgg acaaatacac ttgcgcctgc ttcaacagct ttttgcgctt
      601 cttctagtgt agcattactg tgtcctagag aaacaactac tccttcttca gtcacttgtt
      661 tgacaaactc ttctacacct tgacgttccg gtgctaaagc aatttttttg attaaaccac
      721 ctgaagcttc ttgccattca tggaaagtat ccaaatcagg atccccgaaa taacttgggt
      781 tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc aaaatagatt cctttgattt
      841 tagctcctgg tgcttcctga tacattttgc cgattgtctc agctacatct tttaaacgtt
      901 ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac tccacaagat aacagacctt
      961 cagacatcac tttgatgcct tctgcatcat tatccattac atcatgattc atatagccgt
     1021 gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc gctttggtca atgatgttga
     1081 cttctccctc aggtagttct ttggtgtatt caccaaaaag tccatcggta atttcaagat
     1141 agcctgcatg tttgacacct gacgctaaaa agaatttgtc agcatagata aaagttctca
     1201 ttctattctc tccctttgtt tttctacctc taaagtactc ttatttagag aagaagtcaa
     1261 gaatggtcta caccatttac aaattttttg aaatttgttt cacagcttgt gctaagcctt
     1321 catagaatgc tgctctttct ggttcttctt tacgcttgta tattaagctt ttaagcacaa
     1381 ataaatcatt gaggtaatag tagctattct tctcttgagc ccaatagatt ccttgattca
     1441 cctgttctaa cgcttcgtcg atttcatttt gatggaataa gaaatttcca taattataaa
     1501 aaatttgcga aaattcactt tttgatcgtt catcagctga ttgatgcagc agttggatgc
     1561 ttcgactcaa atagtagcgt gcttcagccg tttttcctgc cacaccatat atttttccga
     1621 tacaactaat cagttgtatt tctgtgtcag aaacatggat tctttctttt tggctagagt
     1681 agcttaatgc ttctttcaaa taataaaggg actgagagat attttgacgc aacgtgaatt
     1741 cgcaaacacc gagataataa taatagcgtt gaaagtcagt tgcttgatac agattttcag
     1801 aaatatcaga agattttagt acttgttcta acttccgata atcacgtttt tggaaacaac
     1861 tatccaacag ttcaaataat ttatcgtttg tccgagtagt ttttcgagag agattcatca
     1921 catgatcaat cgttacccct agccgttgac aaatctgcat catcaccaat acattcggca
     1981 gttcctcatt attttcgatc cgacttaata cactttgtga acaaatatca tgcgaaagca
     2041 ttttttgcgt cattttttgt tcttttcgaa tctctttaat gatcgttcca aaggactcga
     2101 ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt cgcattctat tcgtatttta
     2161 ctttaggaag tcgcaaactt caagaaaaga cttgcaacta aataatatac tttagttatt
     2221 taagaaaact ccactagaaa gagagcctca gtgtgtttgt ctgtggccct cctactgaaa
     2281 aatttggttt ttaaaatcat ttttggcgat aggttgctag gaattctctc attttcttag
     2341 ctgtttgttt caaatcatcc attttaggaa gataaacgat tcgaaaatga tctggctggt
     2401 tccaattaaa tccaccacca tgtacaagta gaatatgatg ttcatgtaaa aagtccaaaa
     2461 cgaatttttc atcatccaaa atattgaatc gctttgtatc aatttttggg aatatataaa
     2521 aagcagcttt tggttttacg gctgaaagac caggaatatc attgattgca ttatagatat
     2581 attctctttg ttcatagatc cttccgcctg gcaggagcag ctcatcgaca ctttgataac
     2641 cgcctagcgc ggtttggata atttgctgag acaatacatt ggaacacaaa cgcatggagg
     2701 acaacatatt cagcccttct atatagcctt tgacgtgtga cttatctcca cttaggacca
     2761 tccagcctac tcgaaatcct gctacacgat gagatttgga tagaccgttc agcgtaacaa
     2821 cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc tagaccatcc atcactaagc
     2881 gatcatagat ttcgtcggaa taaataatca ggttattttg tcgagctact tcaacgattt
     2941 cttctaataa ttcttttgga taaagtgccc cagtaggatt attaggatta atgataacaa
     3001 tagcttttgt ccgactcgtt atttttgatt tgatatcatc aatatcagga taccattctg
     3061 cctcttcatc gcaaatataa tgaacaggtg tgcctccagc taatgcaata gaagccgtcc
     3121 acaacggata gtccggcatt ggaaccagta cttcatctcc attgtccagc aacccctgca
     3181 tacacatcgt gatcaattcg ctcactccgt tgccggtgta gatatcattg atcgtcacat
     3241 tcggaaaatg tttcagttgg caatattgct caattgcttt tctagcagaa aagatcccct
     3301 tggaatctga gtagccttct gagtcacggg cattcatgat caaatcacga atgacttcgt
     3361 ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt caattttaaa atactaatgc
     3421 cctcttcttg catccgctcc gcttcttcta gaacgggacc gcgaacatcg taactgacac
     3481 cttctaattt gttggatttt tcaaattctc tcatcactta gccaatccct catttcaaaa
     3541 agttagaact ttccaaattt tttgttattt actatttcaa tatcatactc catcctagaa
     3601 aattttgcca cttatttagg aaaaacgtaa gtattaaaaa aacacaaaca agcgttgaga
     3661 acttaattgt catgatagtc attccataga ttcgctgccc aatccattgc aattccacaa
     3721 atgattccta gcataaaacc aagcaaaaaa cgtccgaatg caagtccaat caatacacct
     3781 aagatcattc ccagtcctga aaattctccg tcatatttct ccatcttaat aagtcctccc
     3841 atttttgagc ttgacaacta tgtaaaacct cgctatcatt agtgtgttat tattacgcag
     3901 atgtgttgga attggcagac aagcatgatt gagggtcatg tgagcgttag ctcgtgtggg
     3961 ttcgagtccc atcatctgca ctacaactcg tgaagttaat tttgcttcac gagttttttt
     4021 gtttgaacgt ttctttatgc cattatatac atatcactgt atcctttaca aaaagaacgt
     4081 acaatcatgt aaacagcctg aggtgagtat atgaaggtcg gaaacaagct actcgtagaa
     4141 acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa atccctcacc taatactaaa
     4201 gcgagggatt ttttgtatat tatctgttta tcaccgttat tataaaggta caaataccaa
     4261 gcacggttaa tccaaattgc ataaattcat ttttttgtac actcatgctt ttcattagct
     4321 ctttaactgc cattagtact aagatggcga taacaacata aagtatatag tacgtccgca
     4381 ttccggaagc ccatactgtg ggagaaaatc ccataattaa ccttgaacaa aatcccatta
     4441 tcatcaaaat aactaaaaaa atagaagttt tcttattgtc aaaacttaaa taaattccaa
     4501 caattaaaca tagtaataat gccaagacta ctaaagtagc tatccaagta cctggataaa
     4561 aaagtgacag tttcgttcct aactctgtaa agagattatt caggttacta ctactccaaa
     4621 tcattgccct tttatttcca ttgacgtaag taaagctttg acccattgta ttaccaaaat
     4681 aaataatcag gtttaagaaa aacggtaatg cagtcaatat acgcacataa tagttttgac
     4741 atttcctata tgttagaagg aaaattaaga aaaatagcaa taaaaataag ata
//
LOCUS       DACESX010000160         4759 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.162, whole genome shotgun sequence.
ACCESSION   DACESX010000160 DACESX010000000
VERSION     DACESX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.162"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            48..416
                     /locus_tag="IXF88_002490"
                     /pseudo
     CDS             48..416
                     /locus_tag="IXF88_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            516..716
                     /locus_tag="IXF88_002491"
     CDS             516..716
                     /locus_tag="IXF88_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509193.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            850..2076
                     /locus_tag="IXF88_002492"
     CDS             850..2076
                     /locus_tag="IXF88_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509194.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2926..4251
                     /locus_tag="IXF88_002493"
     CDS             2926..4251
                     /locus_tag="IXF88_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1509195.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            4244..4591
                     /locus_tag="IXF88_002494"
     CDS             4244..4591
                     /locus_tag="IXF88_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509196.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
BASE COUNT         1856 a          576 c          902 g         1425 t
ORIGIN      
        1 gagaagtgat aaataggaca caaaatttgg aaagtaggta agtcactatg agagaactaa
       61 ctttatcttc taaggaatta gaaaagaaaa ctgctttatt aaacgaaata tttagtgctg
      121 attgtataat ccaaagttta aaagaaagtt cattttcaaa actaacagat caggaaaaaa
      181 tttcttatat gctaagttta gataatctag gaactaaata tgatgaatca gcaagtagcg
      241 ataagctttt gattgaatta gtatcaagtc ttagtagttt tggaaaaatg aaaagattta
      301 atgtgatatt agaaattttt gcttggttaa tcccttcatt tatttggttt ttttatattt
      361 tcatagttga atctgagtta atagccaagg tctctcttgg actgttaatt ccgatatatg
      421 cattttattt aataggttca acaatttact ttaataactt gtatacacgt attcaaataa
      481 aaaagcagtt attacatgaa ggattaagtg gtaatatgtt aaaaagaatt gcatgggata
      541 aagataataa aacagatatt gagcttaaag aatataaata caattatttc tcgagatgta
      601 tagatagaaa gttactgttt aaaaaagaag gattagaatt tagttttgat gaagatgaat
      661 ttgaaaaatt agtgatggac tggcgtaaga atttactgga tacggtacaa ttctaaaagc
      721 aattgaggtc gaaattccga tacttgaatc atgatctata ttatcgtgtt atgagtgaaa
      781 tttctaataa agataagata tttcatttgg acagtcatgt gaatatgagc ttaacaatta
      841 aacgtattta tgatctttta tatgtttata aacattaatg aggtgctaaa attgacaaca
      901 ataactatga caaaaaaaat agaagtatat gttgatggat cacaagaaga aagggattcc
      961 ttttttgatt ttttttggaa aaatagtaga gaatatgtaa aagcgttgaa tctggtatat
     1021 aaacagctaa tgtttgaaga gttgataaaa gatgcaatta aagaaatgga tcaaggttat
     1081 gctgagagag aagaaagtat tcaaaatcgg attgaaaagt tagcatatac tgatccaata
     1141 gatcaaaaaa aacttgataa agcaaaagct gaattatcta aattgagaaa tgaaaaaaat
     1201 aaagaagcga gagaaatatt aacgcaagcg atagggttga agagtcaaac gagaacacga
     1261 gatgtagcaa aaaaattaga ctttgacttt ggagattttg ttgattccgc aaatatgaaa
     1321 gctagtaaag attttggcaa tgattttatc ggaattgtga ccggacaaag aacgcctaga
     1381 atatataaaa atagaaacaa atatggatta ttacctttta gaggtcgaga tattaaaatc
     1441 cataaagatg ataagtttta tatttattta cctaaaggct ttaggtttaa agtgaattta
     1501 ggtagcaagc caagaaaagc aagagatgtt gcttcagttt tacaacacat tatggatggc
     1561 gattacaaat taaatcaaag taatcttatt tttgatggaa gaaaattatt ttttaatcta
     1621 acaatttctt ttgaaaaaag ctataatgaa atggaattca agaaaggtaa tgaattgatc
     1681 gtaaactttg cagatttgtg tagcggtgaa tgtttaatta atgataaatc tgtaatgtct
     1741 tttggtgata gccaatatat aagaaacttc aaaagtaaaa ttttagcaat gaaatcaaaa
     1801 gaacaaagcc gaggagtctt ttctaagggc ggtcatggtc gaaaaagaaa attagatcaa
     1861 gatcgatggg aagcaataaa gaaaagagaa agtaattttg ttaaaactta taataatcaa
     1921 tgttctgcaa aaatcgttaa aactgctatt cgcaataaag ctcaaattat tcgtatcatt
     1981 gaaccagata aggcttatgg cgaatgggct tactatcaat tgaaagaaca actagtaaac
     2041 aaaggaaaaa gatatgggat agaagtcaga agttagtaaa ttaaacaatc gttgatttgg
     2101 caacgaaata gtgagggaaa atccactcac tgaagggtaa actccgtcaa gttgtgtgac
     2161 cagacggaag gacgaatgtt gttgaggttt tagaaccata taaaacagca tgtttctcaa
     2221 atttttagga taagggaaat tgctagcagt ttgttttaga accatataaa acagcatgtt
     2281 tctcaaatga gtactttaaa ttaattgatg gcgttcctgc tttagaacca tataaaacaa
     2341 catggattta aaacaagaag taagtcagtt ctccttttac tttagtaagg aaaaataaag
     2401 tattctcaat aaaacttaaa ataagcaaac ctttgtttta atagctaatc gaagtaaaac
     2461 ccaaagccat atgaacccct gaatcagcga aagtttatga tgatttggat tactaatagt
     2521 ttaggaatta gatggtaaat ctaaattaaa caatcgttga tttggcaacg aaatagtgag
     2581 ggaaaatcca ctcactgaag ggtaaactcc gtcaagttat gtgaccagac ggaaggacga
     2641 atgttgttga ggttttagaa ccatataaaa cagcatagtt ctcaaattgt ttaaaatgat
     2701 tgtcaagtta aaaaaatctg ggatcatata aaatagtata gttctcaaat atgattagaa
     2761 accctattaa agagcaatat taagccaact tacttaaatc gtttaattaa gaacaataat
     2821 ctcttttagt cagactttta gtctgacttt tttgtatttc caaaatttat tctttacaaa
     2881 aacgaacata cgtttgtaca attacttgtg aatgggggaa taaaaatgtt tgattacagt
     2941 agagaaccgg tgaatgacta ttttttaatc gacatgaagt ctttctatgc tagcgtggaa
     3001 tgtattgaac gaaatttaga cccaatgact acaccgttaa ttgtcatgag tagatcggac
     3061 aacactggtt caggtctgac tctagcttct tcacctactg caaagaaaaa atatcatatt
     3121 actaatgtta gtagagctac tgatttacct tatccattac caaaagaact tcatattgtt
     3181 ccaccacgaa tgaacttata tataaaaaaa aatatggaag ttaatcagat ttttagaaag
     3241 tatgtcgcag atgaagattt attgatttat tcgattgatg aatcaatttt aaaagtgaca
     3301 aaatccttaa atttatttac tagttcagga acaagaagcg agagaagaag agaattagct
     3361 aaaataatca aagatgagat tcagaaagaa acaggtctaa tagcaacagt cggaatagga
     3421 gataacccat tattagctaa acttgcactg gacaatgaag cgaagagtag taaagaattt
     3481 atagctgagt ggacttatga gactgtacct gaaaaaatat ggaaaataaa cgagatgact
     3541 gacttttggg gcattggatc acgcatgaaa aaaagattga atcatatggg aattatgagt
     3601 atagaagatt tagcaaattg gaatccctac acaattaaaa gtcagttagg tgtaatcgga
     3661 ctccagttat atttccatac caatggaatt gatcgtacgg atattgctat tccaccagaa
     3721 ccaaccaaag aaaagtctta tgggaattca caagttctac caaaagatta tacgaggaga
     3781 gaagaagtag aactagtagt caaggagatg gcagagcaag tagccattcg aattagacaa
     3841 cacaattgca aaactggttg tgtacgctta gcaattggta cgtcaatttt ggaagaaaaa
     3901 tcaggtttct cgcaccagat gaaaatacca attacagata atacgaaaga attacaaaat
     3961 tattgtcttt ttttattcaa taagttttat gaggggcagg aagtacgaca tataggagta
     4021 acttatagca gattagtata cacagattcc ctacaattag atttatttag tgatccaaga
     4081 aaaaagattg ctgaagaaaa tttggatagg attattgata agattagaca aaaatatgga
     4141 tttacagcta ttatacatgc ttcatctatg ttagaaggtg caagaagtat tacgagaagc
     4201 actttaatag gaggacacgc aggaggatta ggaggtttgc ataatgattg aatataacga
     4261 tagaggacgt ttaaaatggt cgggatttta tttgtctgat catactgaaa gaatcaatgc
     4321 agacaatgta caacgtgcta atcataattt ggcaaaagaa caaatgacga ctgaagagat
     4381 tgcgaaaata ctgagaactg ctgtcataaa aaatagaccc gtctgcattc aaaaagagga
     4441 acgaaacgct gaaggttact atccagatga tgtagtagga aaaattattg gatatgacga
     4501 gcttggtgta tttgtggaag aagaaaaagt tcactatgat gaaattagga atgtttcttt
     4561 ctcggaatta ttaaaatgga gtcagatatg aacattatta aaaaatacga attcggttat
     4621 attacgtatg aacagctaat agaggaatta tggggatatg gtcaacaatt aattaatgaa
     4681 gtggggattg attgcttttg tttctatatt gaatccggag caggatacca taagaacaga
     4741 tactatatat ttccatatc
//
LOCUS       DACESX010000161         4728 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.163, whole genome shotgun sequence.
ACCESSION   DACESX010000161 DACESX010000000
VERSION     DACESX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.163"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            442..2286
                     /locus_tag="IXF88_002495"
     CDS             442..2286
                     /locus_tag="IXF88_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1509197.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2342..3808
                     /locus_tag="IXF88_002496"
     CDS             2342..3808
                     /locus_tag="IXF88_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1509198.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3845..4687
                     /locus_tag="IXF88_002497"
     CDS             3845..4687
                     /locus_tag="IXF88_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1509199.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1547 a          766 c         1007 g         1408 t
ORIGIN      
        1 tatcaaataa agtttatata aaaaacctca tcctgcaaga aagaaaaaat gaacccattc
       61 tttttatact aggtcttaaa aagacagaac atctgctaga agatagaaga gaataagagt
      121 tatatatgtt ggaagatatt cttagaaaaa agacaaaact tttagaacac gaaaactata
      181 ttgaacgaat aaaatgaaaa aatgattaaa aaatcatttt attatttacg tttacattct
      241 aatatgttaa attgaatata acttaaacag gcatgtgact ggtaatgcag gcaggaccta
      301 aataagttga ataacaatgt atgcgtttca atacacgttt gccttgttgt caatttattt
      361 agtttctgcc tgttttttgt gttgaggatg gccacttcga ctcaaaagtt catcactcaa
      421 aaaagaaaag agggaaacac catgaattac aaacaaacag cagaagaagt attgcagtac
      481 atcggtgggg aagaaaacat atcccatttg gagcattgct caactcgttt acgttttacg
      541 ttaaaagaca acagcaaggt aaatatgagc cagttagaaa aagtggatgg cgttatgggc
      601 gttcgccaaa atgtgcaatg ccaagtaatc attggaaacg atgttgttga agtgtatgat
      661 gaagtgaaag cgttattagg tgacaatgtt tcaaatactg aagaagcacc aaaacaaaaa
      721 caaaaattag gttcagttct attagatttt attatttcta ttttccaacc tttaattcct
      781 gcgattgcag gtggaggagt attgaagtcc ttgttgctac tagcttctat cttagggctg
      841 attagtgacc aatctcaaac ctatcagatt ttaaatttgg tcggcggggc accgctttat
      901 ttcttgccta ttctggtggc aatgactacg gctaataaat tgaaagtcaa tcaattggtt
      961 gcagtatctg ctgtcggtgc attgatcctt cctgaaatga cagctatgtt ggcggaaggt
     1021 gctacattct tatcttttgg attggataat atagcgtatg catctcaagt attcccagcc
     1081 atcttaactg ttttatttta tgctcaactt gaaaaatggt ttacaaaagt ttcaccaaaa
     1141 ccaattcgta ttttctttgt acctatgatg agcttattga ttacagttcc tattgctttg
     1201 ttgcttctag gaccattagg ttataatgta ggaacagctt tctcttctat cattgtgtct
     1261 ttgtacaatc aatttggatg ggttgcaacc ggggtcttag cagctgtact gccattaatg
     1321 gttgtaacgg gtatgcataa agcaatgatc ccttacgctg tttcatccat gagtgaattg
     1381 ggtgcagaac ttctttatct cccggcttca ttggcacaca atatttcaga agctggcgca
     1441 tgctttgctg tcagtatcaa aacaaaagac aaaaaattga aatctacggc tgtttctgcg
     1501 ggtatatctg cattattcgg aattactgaa ccagctcttt acggggtaac gattctgcac
     1561 aaacgtgttt tatatgccgt tatgttttct agcctgatcg ggggagcatt tgcaggaatc
     1621 gttgcacttc aagcctttgc tttagtgggt cctgggattg caagtatcac gatgttcgct
     1681 gacaaagctg acccaatgaa tctggtttgg gcatttgcag tatcggggat ttcatttgtg
     1741 gttgcttttg ctgcagcgtt ggttctttat aaagataaag aagttgtcaa tgaagttgaa
     1801 actaagatgg aaacggccgt tgtttcagat gctgttcttt caagtcccgt gaatggaaaa
     1861 gtaatctcac tatcagaagt aaaagacact gttttttcta gtggtatggt aggtgatgga
     1921 gtagcaatca ttcctgaaga agggatatta gtagctcctg aagatggaga aatcacaatg
     1981 gtatttgaaa cgaagcatgc tattggttta aaaacggcta atggtgcaga acttcttttt
     2041 catattggta ttgatactgt tcagttagaa ggaaaagggt ttgaggcatt tgttcgttct
     2101 ggtgaaaaag tagccaaagg acaaaagctg attgcatttg atattgattt aatcaaagaa
     2161 gcaggattcg accccacagt tatctgtgtg gtaaccaata aggataatta cactatccgt
     2221 tcatttaacc tagaaaaatt ggtaaccaat gatagtgatc tcttagctat cagtccgata
     2281 gcttaaacac aagatagtga aaaaaaatat attgaatgaa ggaatagagg agaatagaat
     2341 aatgacaaac acaacaagtt ttccaaaagg atttttatgg ggcggcgcga tagcagcaaa
     2401 tcaagtagaa ggtgcttgga atgtagacgg taaaggtctt tcagtagcgg atatggctac
     2461 ttataaacca aatttagaca acaaagattt tgctgctcat atgcatgtca ccctgcagtc
     2521 tataaaagaa gctagtgaag acaaatctga taaatggtac ccaaaaagaa ggggcgttga
     2581 tttttatcat cactataaag aagatttagc tttatttgcc gaaatgggct ttaaaacctt
     2641 gcgcctctct attgcttgga ctcgcttgtt ccctacagga gaagagctgg aaccgaatga
     2701 aaaaggggtt gcattttatg aaaacgtctt taaagaaatg caacgtttag gaattgagcc
     2761 gattgtgaca ctatctcatt atgaaatgcc attagcttta gcagttaaat ataatggctg
     2821 ggtggagcgt cgagtaattg atgattttgt tcgcttcgct aacgtttgtt ttgatcgctt
     2881 cggcaaatac gtaaaatatt ggctgacatt taatgagata gacagcatcc accgtcatcc
     2941 atttacaaca gctggaatta tccaagagaa aagcgcagaa ggtcaagaag ctcaagatat
     3001 ctatcaagca ttgcatcatc aatttgttgc gagtgcgtta gtgacaaaag atgctcatga
     3061 aaaaattcca ggaagccaag ttggttgtat gttaacgaaa ttgatgactt atccattaac
     3121 ttgcgcacca gaagacgttg agctgacctt gaaaaagaac ttagaaaata atttttatgc
     3181 agatgttcaa gttaagggag aatatcctgc tctaatcgaa aaggatttgg aaaatcgggg
     3241 aattcagatt aaaatggaag atgaagatcg agaaatttta aagaaacata ccgtagattt
     3301 tgtttctttc agctattaca tgtccatggc agaatcagct gatccaaacg cagaacgcac
     3361 accgggaaat actgttttag gggttaagaa tcctcatttg ccttcaacag attggggctg
     3421 gcaaatcgat ccaaaaggtt tgaaaatttc gttgatcgaa ctttatgatc gttaccaagt
     3481 accgttgatg attgtggaaa atggtatggg agcagtcgat caggtagtag atggtaaaat
     3541 ccatgatccg taccgcatcg actactttaa agcacacttc caacaaatga aagaagcaat
     3601 cgatgaaggt gtagatttga tagcttatac tagctgggga ccaattgatt tagttagtgc
     3661 aggaacttct caaatgtcta aacgatatgg atttatttat gtggatgcag acgatgaagg
     3721 aaatgggaca tataaccgct tgaaaaagga ttcgttcaac tggtacaaag aagtgatcga
     3781 aacaaacggt gcatccttat tcgagtagtc tcgatctttt taagcagcga tagggggata
     3841 atgagtgatt tcaataaaaa aagtattgaa ttcaagtgtg gtacttgttg aagaaaatgg
     3901 caaagaaatg attgcattag gtaaagggat aggctttgga cagaaagctg gggatgaaat
     3961 atcaggagat aaagtagata aaatatttct gccttttgaa gaaaagaaat cttcacaact
     4021 attagagtta gttgaagaaa tacctattga attttttgaa atcactcgta ctatcgtttt
     4081 tgaagcagaa aaaggattag ataaaaaact gaatcctgga gtctatttaa ccttaacaga
     4141 ccacctgcat tttgcagttg aacgcgcaca aaaaggggta aatattgcaa accgtttgta
     4201 ctgggaaata aaaatctact atcccaaaga atttcaaatt gggaagtacg cactcaccct
     4261 tttgaacgag caatatggaa ttgatcttcc taaagaggaa gcctcaaaca ttgctttcca
     4321 tttgatcaat gcacaatcgg aagatcctga tagcggcgct ggattcaagt atgcaaaaat
     4381 gatcggcggg atcgtcaatt tggtccggta ttcgttgcag aaagatatcg atactaattc
     4441 gatccattat gcccgcttca ttacacatgt acgtttcttt gtagaaagat attattctga
     4501 tgctttgttg gatgatggtg aggaagaatt ctacaatcaa atgtggatct tatacccgtc
     4561 tgccatggaa agcgcagcta agatcaaaga ctacattgaa aaagtttatc ataaaacaat
     4621 tccgaacgat gaagttgtat acctaggtgt ccacatcaat cggttgatga aacatgctaa
     4681 tgggtgagcg ctcagtaata ctattgatgc ttttaaaatc tttagaga
//
LOCUS       DACESX010000162         4625 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.164, whole genome shotgun sequence.
ACCESSION   DACESX010000162 DACESX010000000
VERSION     DACESX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.164"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            198..473
                     /gene="rpsP"
                     /locus_tag="IXF88_002498"
     CDS             198..473
                     /gene="rpsP"
                     /locus_tag="IXF88_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1509200.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            485..736
                     /locus_tag="IXF88_002499"
     CDS             485..736
                     /locus_tag="IXF88_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1509201.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            861..1652
                     /locus_tag="IXF88_002500"
     CDS             861..1652
                     /locus_tag="IXF88_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509202.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1822..2343
                     /gene="rimM"
                     /locus_tag="IXF88_002501"
     CDS             1822..2343
                     /gene="rimM"
                     /locus_tag="IXF88_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1509203.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2333..3094
                     /gene="trmD"
                     /locus_tag="IXF88_002502"
     CDS             2333..3094
                     /gene="trmD"
                     /locus_tag="IXF88_002502"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1509204.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3230..3577
                     /gene="rplS"
                     /locus_tag="IXF88_002503"
     CDS             3230..3577
                     /gene="rplS"
                     /locus_tag="IXF88_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1509205.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            3835..4128
                     /locus_tag="IXF88_002504"
     CDS             3835..4128
                     /locus_tag="IXF88_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1509206.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1640 a          791 c          922 g         1272 t
ORIGIN      
        1 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
       61 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
      121 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
      181 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
      241 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
      301 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
      361 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
      421 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
      481 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
      541 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
      601 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
      661 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
      721 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
      781 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
      841 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
      901 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
      961 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
     1021 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
     1081 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
     1141 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
     1201 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
     1261 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
     1321 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
     1381 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
     1441 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
     1501 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
     1561 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
     1621 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
     1681 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
     1741 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
     1801 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
     1861 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
     1921 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
     1981 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
     2041 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
     2101 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
     2161 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
     2221 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
     2281 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
     2341 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
     2401 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
     2461 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
     2521 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
     2581 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
     2641 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
     2701 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
     2761 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
     2821 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
     2881 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
     2941 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
     3001 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
     3061 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
     3121 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
     3181 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
     3241 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
     3301 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
     3361 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
     3421 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
     3481 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
     3541 cggaaaagct gctcgtatca aagaaatccg tcgttaatca ttgttatcag actgagaaag
     3601 aacccttggt attcaagggt tcttttttct tagcatgtaa tttgaacacc ctgatccagt
     3661 tttttcttta attattggta ggggagaata atggattaga ataaatctac ctataagtag
     3721 tagtaaaaaa aagagcacta tgataatatc tttcttaaga gtaaccgctt taataaaaaa
     3781 tccagaatat tctaacataa atggagtatt cttagaatca aaaggagaaa gaagatggtt
     3841 tatacttata cggagcaaga aaagatgaaa gttttagaca cattcatgaa tgatggaaaa
     3901 gtaaaacaag taccaagttc aacaaaaaag aaatacatcc tattgaaaga agtcttgaaa
     3961 cgctttgacc atggagtgac ttatacagaa acagaggtta attcgatact gctgaacgta
     4021 ttttctagtg gcgattatgt cgaacaaaga cgttatctaa tcacatttgg ctttttcaaa
     4081 agaagttcag acggtcgggc atatcagatg atgggaattg aaaattagaa gttttaattt
     4141 atactcattg aaaaaagttc aagtatgcga tattggttct acaccgcata cttgaacttt
     4201 ttttgtcatc tgtatctata atacaaagcc aagaacgtta ttcaaaatat ggatagctat
     4261 gataaaagct aatgagcgat tcgcagttaa ataagcaatc gtcagtaaaa tcgttccagg
     4321 agcgtacatg aaaaaatcaa ttggatgcgt tacatgaata aagataattc tcttttattt
     4381 gtacgaataa ataccaaaac gtacctccta aaattagatt ttggacagat gccctttaaa
     4441 aagataacaa gatttcgttc ttgaaacaat atatatgctt ttattccaat gaggttgtga
     4501 aaacagtatt ttgtcctgag cgttagaaaa ttgaaaatgg ggaatagcaa ctcgtacttt
     4561 accatttttt aagtgaatac aagatcagct aattgaacac cgtttattcg tgatgaggga
     4621 cacaa
//
LOCUS       DACESX010000163         4603 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.165, whole genome shotgun sequence.
ACCESSION   DACESX010000163 DACESX010000000
VERSION     DACESX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.165"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            43..113
                     /locus_tag="IXF88_002505"
     tRNA            43..113
                     /locus_tag="IXF88_002505"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75..77,aa:Cys,seq:gca)
     gene            134..218
                     /locus_tag="IXF88_002506"
     tRNA            134..218
                     /locus_tag="IXF88_002506"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:168..170,aa:Leu,seq:caa)
     gene            280..352
                     /locus_tag="IXF88_002507"
     tRNA            280..352
                     /locus_tag="IXF88_002507"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:313..315,aa:His,seq:gtg)
     gene            362..433
                     /locus_tag="IXF88_002508"
     tRNA            362..433
                     /locus_tag="IXF88_002508"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:394..396,aa:Gln,seq:ttg)
     gene            451..535
                     /locus_tag="IXF88_002509"
     tRNA            451..535
                     /locus_tag="IXF88_002509"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:caa)
     gene            790..1665
                     /locus_tag="IXF88_002510"
     CDS             790..1665
                     /locus_tag="IXF88_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1509207.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1769..2533
                     /locus_tag="IXF88_002511"
     CDS             1769..2533
                     /locus_tag="IXF88_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1509208.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            2550..3962
                     /locus_tag="IXF88_002512"
     CDS             2550..3962
                     /locus_tag="IXF88_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1509209.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            3979..>4603
                     /locus_tag="IXF88_002513"
     CDS             3979..>4603
                     /locus_tag="IXF88_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1509210.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIY"
BASE COUNT         1462 a          717 c          994 g         1430 t
ORIGIN      
        1 ctataattta ttctttattt tatagattga taatagtata atggcggtat agccaagtgg
       61 taaggcagag gtctgcaaaa ccttcatcac cggttcaaat ccggttaccg ccttagtact
      121 aataaatcaa cttgccggcg tggcggaatt ggcagacgcg ctggactcaa aatccagtgc
      181 ccgttgaggg cgtgccggtt cgaccccggc cgccggtatt gactaagatg tcttcatctt
      241 agttttttat tgagaaatta aataacatct tttatgatgg cgaatgtggc gaagtggtta
      301 acgcaccgga ttgtggctcc ggcattcgtg ggttcgattc ccatcattcg ccctttattc
      361 ttggggtata gccaagcggt aaggcaaggg actttgactc cctcatgcgt tggttcgaat
      421 ccagctaccc cagttattat ttttaaattt gccggcgtgg cggaattggc agacgcgctg
      481 gactcaaaat ccagtgtccg ttgaggacgt gccggttcga ccccggccgc cggtataatt
      541 atcaatatag caactttctt atagagagtt gctatatttt ttttgttttt atgtaattac
      601 ctccaaatca attcgataca agtattatca taaaacgagt aaaaaagata ttgataacgc
      661 ttttattatg tgttaaacta tttttgtaaa ttatgtaggg tgctactatt caattaggca
      721 cgacctaaga aagggttctt tcttaggtcg ttttttgtct ttaaaaattg tttggagaca
      781 aaaatatgta tgaaggagtc tgcaagttgt tttgttttat catatagagg aggtgaatta
      841 acggtgtatg tgatgaaaaa gctgaatcat aatgctgtat tgatcgatga cgatggaata
      901 gaaaaaattg cgatggggaa agggattggc ttttctgcta aagtgagtaa gccatttgat
      961 gttgaggatg ctgacaaatt atttgtatta gattctaaag aaaagatcaa acgattcagt
     1021 gagatagtct cgcagattcc aatcgaattt atggattttg cggaagaagt gattgaactg
     1081 ataacagcta ggatctcctc tccattagat tcaaatatct atattacatt gacagatcat
     1141 atttattttg ctattcaacg aaaaagagaa gatagagatg tcacagcaat tatgttgcca
     1201 gaaatgagat tcctttatcc aaatgaatat gatatagcag tagaagtggt cgagcgaatc
     1261 aatcatcgat atcaaaccga gttagggtca aatgaagtag gattcatcac tatgcatatc
     1321 attaatgctg agctaggaga gcctaatagt ttaaagagct taaaaatagt agaaatgacg
     1381 accacgattt tgtcccatct agaaaaatcc tattcaaacg tttatgataa agaatcactg
     1441 acttatcatc gcttaatggt tcatatcaaa ttcctggtta aacgcttgat ttatcaggaa
     1501 gaaacgccag tagaaaatat tggttttttt actgaaacat ttaaacatag caaagcgtat
     1561 tgtacagcac taaagattaa agatatcatc gaacagcaat ttcaacttgc cattcaagaa
     1621 aatgaaatca tctatttggc aattcatcta gcaagaatct attaaaataa gggcgtaact
     1681 atctataggc gcaaccaaat gacacatagt aaatggacta tgattatttg gttgcgccta
     1741 ttttagtaga aaaaagaagg agaatgaaat gggaaaagtg atttttaatt cggatgattt
     1801 tggctatagc catggtgtga attatgggat tatggatgct tatcagcgag gaattttgac
     1861 atcaactaca ttgatggcaa atatgccggg tttcgagcat gctgtgaaac taagaaagga
     1921 aatgcctagg ctaggtgtag gagtccattt aacattgacg tgtggcaaac cactcctcaa
     1981 aactgtcgat actcttatcg aaagcggaga gttcagatcc ttatcttatt atcagcatcc
     2041 atttaacatt gacaaagatc aactgtatca ggaatggaat gctcagattc aaaaaattta
     2101 tcatgcgggg atcataccaa ctcatttaga cagtcatcat catactcata ctttggatga
     2161 aaatcaggag gtagttgtta cattagcaaa aaaatatgat ttgccggttc ggagcaactt
     2221 tgaacgaaaa gatgaagttc gtcatgtcga ttattttgag ccttgtttcg atgtcgtagg
     2281 agaaactgat gaaaaaaaac gacaaatcaa tacgagtgtg gaagaatatt tggcaagttt
     2341 actcatgata ttgagagaag ataaaacgat cgaagtcatg tgtcacgcag cgtatttgga
     2401 tcaggaattg ttaaatggtt caagttttgt gtatcctcgt acccaccaag tcgaattttt
     2461 aattcattca gcatttgctc aaaaagtgaa aaatgatcct ttgattgagt tagtaacata
     2521 tgaggatatt taatagagga ggaataaaaa tgaatgttaa atcaaatgtg cagaagcttg
     2581 gaagagcgat gctacttcca gttgcagcga tgccattagc gggattgatc atgcgtttat
     2641 cagctgacga tatgctgaac atcccagtaa ttggtgcagc tggaaatgcc gtttttggaa
     2701 acttggatat cttatttgct ataggagttg caatcggttt tgtcaaaaat aaggacaaag
     2761 gaatacctgc acttactggt tttttagcga ttgctacctt gaaaaaaggc ttagaaatta
     2821 tggacccaga agtgaatatg gggatttttg gtggaattat ttccggttta gtagcggcat
     2881 ggacgtataa tcgctttaaa gaacaaaaat taccagtagt tttttcttat tttgcgggcg
     2941 aaaaattccc attgactatg gtgatgattc tacaaacaat cgcagctgtt tttttgggct
     3001 ttatctggcc gctcatccaa attgggattg ataattttgc aaaactgctc gttgatatgg
     3061 gagcattagg tgtggggata tttatgtttt tgaatcgttt gctgatcccc tttggactgc
     3121 accatgtgtt gaatacgtat atttattacg atttaggaag ctatacatct cctagcaagg
     3181 aagttttcag aggagagatg acacgcttta tcaacggaga tcctcaggct ggactgtttt
     3241 tatcaggttt ttttgtagta atgatgtttg gtataccagc tatatgtcta gctatctata
     3301 aagcagctta taaagaaaat aaagagatgg tcaaaggaat catgggaagc ggtgcaggaa
     3361 cttcatttat tgcaaatatc actgagcctg ttgaatttag tttcatgttt ctttctccta
     3421 tgttgtacgt gatccatgca ctttttgccg gtttagcagg gattgtctgt tacttattca
     3481 atatacgaat tggttttaca ttcggagcat gtattgttga ttatctcatc aattttcgaa
     3541 tcgctaccaa tgctattttg attcttccaa tcggaatttt cttttttgcc ttgtattacg
     3601 ttacttttta ttatttgatc aataagagaa acattcagac attaggacgt gaagctaagg
     3661 cagagtttgg caatgaagta accttagaag aaacagagtt agggttagca agtaaaaatt
     3721 atgaatatat ggcaacaaaa atgctacaag cgtttggagg aaaagccaat atcctggatg
     3781 tctatagttg taatacacgc ctacgggtag aggtagtgga tcccaccatg gtcgaggaac
     3841 agcgaatcaa gcaattaggg ataagcggga tcattaaacc aactgaaaaa aattatcaaa
     3901 ttatcatcgg cttagaagtg acttatgtta tggcagaatt caataaatta ttagaagaat
     3961 agaaaggaaa agatgatgat gaaaaaacag attatcacta tagctggagg cgggagtacg
     4021 tatacaccag gaatcgttca agccattttg aacaatgaaa aacggttgcc attatctgaa
     4081 atacgtttat atgatattga tgaagaaaga aatatggata tgtacttgat tgttaaattt
     4141 atgctgaaaa gaaaaggttt ttcaaatatt cgcattcgtg caacggatga tccaaagctt
     4201 gcttttacag gatgcgattt tgttttctca caaattcgtg ttggaggatt ggagatgcga
     4261 gaaaaggacg aaaagattcc acttaaacat ggattagtag gacaagaaac gtgtggactc
     4321 ggaggatttg cttatgggat gcgctcaatc aaaggtttat tggaaattgt cgatcatata
     4381 caagactatg cccccaaagc atggattttg aattatacaa atccagagtc gatcgtttct
     4441 gaagctgttc gtcgtaaata tccgaatgta aaaatgatta atgcctgtga tatgactatt
     4501 tcaattgaag aaacgattgc agtgaattac ggttatgatc gtaagaattg gatcgttacg
     4561 tattatggtt taaaccactt tggctggtat acatcgattt atg
//
LOCUS       DACESX010000164         4486 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.166, whole genome shotgun sequence.
ACCESSION   DACESX010000164 DACESX010000000
VERSION     DACESX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.166"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..282
                     /locus_tag="IXF88_002514"
     CDS             <1..282
                     /locus_tag="IXF88_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509211.1"
                     /translation="EYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFINKSD
                     SLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            279..521
                     /locus_tag="IXF88_002515"
     CDS             279..521
                     /locus_tag="IXF88_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509212.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            539..841
                     /locus_tag="IXF88_002516"
     CDS             539..841
                     /locus_tag="IXF88_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509213.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            911..2974
                     /locus_tag="IXF88_002517"
     CDS             911..2974
                     /locus_tag="IXF88_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509214.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            2974..>4486
                     /locus_tag="IXF88_002518"
     CDS             2974..>4486
                     /locus_tag="IXF88_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1509215.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDE"
BASE COUNT         1716 a          697 c          919 g         1154 t
ORIGIN      
        1 gaatactctg agagtgtatg gcatccagat cctgtacaaa taacagtcaa gaggcaaaaa
       61 gactataaag gtttagaaag atacaaaata gtagattcag ctggtgttag ggaatttata
      121 aacaaatctg attctttatt tattgttgtt ccctatgaga agaatggcgt aaaaaaaata
      181 atttttcttg gatctagcaa ggatgaaaag cgaacgaaag agaaattgat tcacgatgga
      241 tcacaatcca ataaatataa gttacaaaag actagcgagt gattgctagt ctttttattt
      301 gtcctgaagg aggaatcaca gtggcaaaag ctaaaacgcc atttgaaaaa gccgcgtcta
      361 aaatcttaga acgccgcggt gtatctgaaa cacagtggaa acaagaagtc cttgaaaaag
      421 cacaatttga cctattacgg ggaaaagaca aggacttaga aggatatatc tatcaacgtg
      481 aacgtgaaaa gcttgttttc gatgaaatta ttaaacacta aaaggagcga acaattttat
      541 gtttgaatca attacaatga attttttaac acaaccgaaa gtagtagctg cggttgatat
      601 ttttagagca ttacaaactg ccgcaactag tttaaaaaac aatctgatga tttttgctgc
      661 tgctgcggcc gttgcttcca ttgttattgg gggtcttttc ttcctatttg gacgaagagg
      721 agctgaaaat gggaagatgc acgtaggcta tgtattgatg ggggtaggag ttattttagc
      781 agcagcttct attgtggttt cgttatttgg attgtttgga caaacacctc aaggattata
      841 agaaagtaaa aaagtaggag taagaagtag tcgatacttt ttactccttt tttatgaaag
      901 gagaatactc atgtacttta cgattgcagg caataccaaa acagataaag agttaataaa
      961 aaaagcaacg aaggaagtag gaaagcgtac ttctttttta ccgaaaacaa ttaaatattc
     1021 tgtcattgaa acggaagaac agctacatgt gttagggaaa gtactggaac acgaaagtcg
     1081 atcttctgtt aagttgactc tcacgttaca tgacacagaa ggaaaaatag agaaagctgg
     1141 tacagaatac gtttttggcc cactggatta tcaaatttca gaaggtttct atcctacatt
     1201 gatggattta atccgccttg atgtgtttaa agaaacggat gaaactatga gtgtggaaga
     1261 gtcttatcga aacgaaaaac ttttacaagt tttagaagaa gagttagaga gcgaccaaag
     1321 aaaagtgaat caagggagtg aagtcgaagt aaatgaagaa gtaccatggg aacgaccaat
     1381 atttccaaag aaggagtcaa aagaaaaaga gacagtctat gttgacgaac aagctgatat
     1441 tccgactcag aaatcgatat ttgaggaaga gaacgatgtg atgggtcaat tggaggtaga
     1501 tccctttgaa gaaacaatgg aggatgaaga gacggccgaa gtagaagttg atccagttga
     1561 agcgaatgaa gagtcaacat ggactgaagg ggattctcag gaatatattg aaccacttat
     1621 cagtgaagaa gtagaaaaaa acgaaataga gatacaaaag gcaactgtat ttgattatat
     1681 tttggatcaa tatccgccag agcatgaatg gatgaaagaa aaaaacaaat tatttttaaa
     1741 tgagctttat tcgaaatatc aattacccca aacctatcaa gcatttttag agagtaaaga
     1801 agaattaatc tcacagggta aaggcacact tttagaacag ttagagtctg tccaatcaac
     1861 aaattggcat gagcttgcac aagaagattt attagccacc tttgaagaac gtcaacaaga
     1921 aacagatcaa gaaattaatg agtatgcgac ggaacagtcg tctaattggg agaaagcaaa
     1981 agaggaacta aataaagaag aacagcgagt gattgaagaa gaagtacaaa aaattaaaga
     2041 gcgatacgaa aagaaacgcg acaatgcgta ttccttgtgt gtagcaaaga tcaataattt
     2101 tactcagacg aatcaagaag cgttatcgaa agaaaaagaa aacttgttaa aagaacgtat
     2161 taaagaacga aaagaagaat tttaccgagg tttgcgtaca gataagctaa agattagcaa
     2221 agaattaaat gaacaactga ataagctata tcaaacaacg actgaagttc ttatggaaaa
     2281 atacgaaaag atccagcaag aattacaagt gcaaattcca aaatggaaaa aagagtatga
     2341 aattcaacaa caagaattga aagaacaaga agaaaaacaa cggcaaaaag aaaaagagcg
     2401 cgaacaatta gaactaaaaa aacgtgaaca ggatcttgcg gaacaacaaa tggcaataga
     2461 aaaacaacgg ttaaaccaag aaaaggaaaa agaagcagct gctcgtaagg aacgtcaaga
     2521 acaaatggat acattgcgac aagctcagtt aaattatcta gcacaacctc aaccaacgcc
     2581 ttcagtagct gctgttcaaa ccccaatgaa caaccaaaca aatctaactc cttcaccaca
     2641 gcgttccttt aacggatgga tggtgggatg tattgcaagt ttaagcttgc ttctaggtgg
     2701 aggaacagta tttgctttta atcatatcaa tcaatcttca caggctgatt ctgtggccac
     2761 aacgacgtca atcaaggaag aagcccaaga acaagcaaga aaagagtatg aacaacaatt
     2821 agaagaatta ttgaatcgta ccaatcagtc aacaacagag tcaagcatgg aaaatagcaa
     2881 agagagtgat tctagtaagg ctaaagaagg atcaagcgat tcaactgtac aatcaaacac
     2941 ttccgaaagt tcagaatcaa atgaggtgaa ataatgcgtg ttagagaaac aacgaagcga
     3001 aaagacaaac gattcaattt agaaaagaat aggtatcttt ctcatttcta tcgctatact
     3061 ccctcaggag aagagctacc agaaaaacat gacaaacgca tttcgaaaaa gattttttat
     3121 agtggacttg tatacctccc tttagagtat ttagtaacaa gagtaccaga agttttcaac
     3181 cgattggaaa ttgttaaggg aagcttacaa ggaataaatc tgtttcaatt acaggaaatg
     3241 ccacaggtct ttcattttga ttcagctggt ttttggaatc catttactcc tacaactagt
     3301 ttgggtacac gtttagcaat ttcgacagtt tgtgtggttc cagctgtact tatgggattg
     3361 aaaatggaca tgtggtttcg tcctctagca gatggacaaa aaggggataa tcgattaatg
     3421 accttaaaag aaatccaaca gatttatccg caaatacctg aaacaaaaac tacttttcca
     3481 ggttatggag gtattccaat cacacattac aaaaaatatt ggtacattca aacagatact
     3541 gttaatacgt gtattgttgg tacctctcga tcagggaaag gtcagattga agtgttagcc
     3601 accattgaca atttatctcg tgcagaaaaa caaagttcaa tggtcgttaa tgatccaaaa
     3661 acagaattat atgttggtag taaagatgaa ctagaagcta gagggtatga tgtatatgcc
     3721 tttaatatca ccgatccact gcaatcaatg agtgataatc cattggcact catcgtgaaa
     3781 tactggaaac gaggagacat agatactgca acacagttaa ccaatacttt cacaaatacg
     3841 atttattatg atgccaacgc ttcagataac aaatacttta acgaaaacgc gcagaaagct
     3901 gtcaatgcgc ttatttttgc gttgttagaa caagctgatc aaacaggaga ttactccaaa
     3961 ttgacaccga ataatctcgt tgagcttctt tctgagattg gcggttttaa ttattatcca
     4021 gatccagaca atgaattcaa acagttgaat gccttagatg aatttatgaa tcagttgcca
     4081 cctggaaaca ttgcaaaaaa acaatatggg gcaacgaaaa ttggatctga taaagcgaag
     4141 ggaaatattt tatcaacggc aatcacggga ttgaatccat ttactctaac gaaaattgca
     4201 aagatgactt ctcaatcaac gattgattat aaatcggttg ggtttccaaa atacttagat
     4261 ttgaaattac atgaaagtct tttaaatcaa cgagtcgtag tagaatttta tcataaggga
     4321 aagaaatttc atgaagaacg tgtcaaagta ggctataaag gattttgtga aatcaatttt
     4381 gattgtcatt tagttgcagg tgatcaagtt tgtttaaaat acgatcatca aggaaaagaa
     4441 tatgtggctt ggtatgaatt ttctcaaaga gtattgaaag acgaag
//
LOCUS       DACESX010000165         4414 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.167, whole genome shotgun sequence.
ACCESSION   DACESX010000165 DACESX010000000
VERSION     DACESX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.167"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..105
                     /locus_tag="IXF88_002519"
     CDS             <1..105
                     /locus_tag="IXF88_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1509216.1"
                     /translation="MEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            1492..2415
                     /locus_tag="IXF88_002520"
     CDS             1492..2415
                     /locus_tag="IXF88_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1509217.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            2431..2970
                     /locus_tag="IXF88_002521"
     CDS             2431..2970
                     /locus_tag="IXF88_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1509218.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1638 a          679 c          742 g         1355 t
ORIGIN      
        1 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
       61 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag cttaaatcat agataccgta
      121 agggatttta ttctttattt aaaactttgc aacagaacca tcatatcaat acaatgatta
      181 cgctcgttat gtaataaaat aatcagaaag tgaaactatc tcatgaataa aaagatttta
      241 gctgtaatta tttcaataat agttttaatc actatgttct tcatatttag gctaattttc
      301 aatataagct tacaaaaaag tattctttat ctaattccta tagcatttat cttagcaatt
      361 tttagaagta tttacggaaa taaaaactct aaatagctaa aaataaatca tactaaattt
      421 tacaaaaagt ggctagcatt ttctagccac tttttgtatt gtggagcgtg ggttttgagg
      481 gggattcaag ggggagcttg cgcccctttg acaagcggtt atttgcacca aatacggagg
      541 tatttggcta gcaaatatag agcttgccaa acccttttag tgagttttgt agaaagtacc
      601 tttaaaggta ctttcacatt acgaacgata cgtccgtagg acgtactgaa agggtgggac
      661 ttatgagcaa attaatgcaa aatttggcta gcaaaaaagc taaaaaatat atgctaataa
      721 tcgagcgaaa taaacacttt ttcaagtgag cgaatccgcc atgacaaagc tgaagcagtt
      781 tatttcagct ttgtcatggc gtgtgccgac ttttttaaaa gtctaggcac acgagcaaat
      841 atctttatca agctctaatt tgcgttttaa gacgtttgaa acgcctaagt atataaatat
      901 ataagaatag gtttaagagt gcttaaatcg aattttagag ggcatttaga caattttagg
      961 gttgttaggg cttgccctga ccgcctgtaa ggcgttttaa gaacaagata agggatttat
     1021 actaggactg tcttaaaatg tcttaaacga acgctcagag cttctcaaag ggttccttat
     1081 gaacggcttt tagcaaagga gccggaggcg actgcgcagt ataatgtgag agcagagcga
     1141 tcacaccttt ttagcgaacc gtagggagca aggtttggaa ttggcagttt tgataaattc
     1201 gcgaacctgt ttagagaaaa aaggagtgat tttttaggcg ggaaaattag cctcccctct
     1261 ttttatatct ctttttatat ctctttttat atctctttta tgtcggctgt gtccttactc
     1321 tcccaagcaa ttcagagttt tttcaagtag gttttctacg cttttcaagt aggttttcta
     1381 cgcttttcaa gtaggttttc tacgcttttc aagtaggttt tccgacatta ttgagtattt
     1441 ttataaagta tgttactttt atttcatact ttataaaagt aggagaagct tatggcaaat
     1501 gaaattgtaa aatatgaaaa tagattaaat catattcctc tgagaaaatt taatagtcgt
     1561 gaaatgaact tatttttttc aattgcttca cgtgtacgtg aaaaaggtgt agatgagatt
     1621 attttttcat ttcacgattt gaaaaaacta agcggttata cagaacgtgg ggaacaattt
     1681 ataaaagatc ttagtagcac gtatgacaaa ttattaagta ttaatgcttg gaatgatgat
     1741 ggaaatactt taactaaatt tgttgctttc acaaaatata gcattatacg agataaacaa
     1801 cttgttataa tttcagttaa tccagaattt aaaggacttt ttaatcaatt aaagaactgg
     1861 acaagattta atctagagca atttactaat ttggatagca catattcaaa aactatgttt
     1921 cgattattaa aacaatatcg aactcaaggc tggactgaat tttccaaaga aaaatttttt
     1981 gatttactag atattcctaa aagttattgg aatagtccgt caaatattga caaatttatc
     2041 ttaaggccaa ttaaagaaga actcactccc ttatttaaag gattaactat tcgtaaaaaa
     2101 tatggtaaag gtcgaggtaa acctgttatc ggttatcgtt ttacttggaa agctgaaatt
     2161 aaccatgctg acgatttttc caaagggaaa caagaagatt tacgtataaa attatttaat
     2221 attgatcaca atggagaatt aacacgagaa gaaaaatggc gcgctaaaga tcgaatacta
     2281 aatcttccct taggcactca tgaagctgat tttaaacaac aacaacaaac tgaagaaaaa
     2341 caggcagaaa aacgagaact ttcaaacaaa ctcaaacaag atttactagg aaatttacaa
     2401 aacttattcg attaagctta aggagtattt atggcagata aaacaatcaa agaacttgcg
     2461 gaagaattag gagttagtaa aacagcaatt aacaaaaaag tttctgacaa aaatcgaaaa
     2521 ctttggtttg caaaaaacgg aaacaagttt gtgattaacg aaaccggtca aaaagctata
     2581 aaatcaatgt ttttagtcga taacgcaaac ttaaatcgaa aactagttgg cgaaaaatca
     2641 aaaaccaaaa accataaaaa caccaaccaa gtttccgaat taaaactcct ttctttccta
     2701 gaaaaacagt taaatcgaaa agatatacag ctacaagaaa aagataatca aataaaacaa
     2761 atgcaaaaat tactcgacca gcaacaacaa ctcactctac aagcgaacca acaaattgag
     2821 aacctacaag aacagctcca tttaacttat acagaagaaa ctccaacaac cgaatcaact
     2881 gccttttctg aaaaaaaagc agacaataca caaccactta ttgaaaaaaa gtggtggcaa
     2941 atttggaaat ctaatagaac caatctctaa aattttctag aagttagttc ggatagtaga
     3001 ggaaaaagtt gtactacctg atggggagat cactcaaatt tttatgagaa agaaaaaata
     3061 gtttacaact gtaaacaatc aatgtttata gttgtaaact atcaaactat atggtgactt
     3121 attgcttaaa ataggttgaa atctcactca atttttggtg gaaaaacaaa ataaattcta
     3181 atcgattttg ttgaaaaagt cataaaaaaa caaatgtgat tttaacaaaa tttcaaaaaa
     3241 atcgtttgaa atttcgttta ttttttttga gaattttgat catcgtaggg tcattgtaga
     3301 atcattatga gatcatcgtg ggatcatcga ataaagtatg ggaaaggctt gcggtgcttg
     3361 gtattaaggg cattctccgc ctcccaggtt cttgaccatt ttctctcaac gtggtaaaaa
     3421 tcccacttat aagagaaaat ggtcaagaac ctaaacggtc ggcgaagaaa acataccaag
     3481 caccacaagc aaaaaaagtt agagtaactt ccaaaaaaga aaaatttttt agaacaactt
     3541 ttgaaaattt aattttgatt tatatccgtt caatttaaac atttgataaa ctgaaggcta
     3601 gtatttaaaa tgtgtataca agtggtagcg ttaaaaaaag tggaggtata acctatgtta
     3661 aaaaaaactg tgttggtact gggttctatt tctctaggaa tattaggttt tagccccatg
     3721 ctattatccg taattggaaa aaacaattcc aacggagaac ttaaattaaa ttcaaattat
     3781 tacaacagtc ctaacgaaga attgtttgta aaagagacgg ataaataata aaaaagactg
     3841 ctctttcgag cagccagtta aaaaataatg agtgtttatt cttgaaaaag caacgcgctt
     3901 atgaagcgct accaaaaatt tatcatattt tatgaaaaat aacaccccag taaaaaatga
     3961 ctggtaaaaa ttcacaaaga aacggaaaga agcaactgca gaaaatcaag ccttaaaaag
     4021 agcttttgac ttagaccaat cgaaaatcac aaagtgagcc cctgtatccc ttggtagaga
     4081 ggctacttgg catagtacac attatgtcaa caagaattta atataacata tttttctagt
     4141 ttgttataac aatgccactc taacaaaaat gtaagcatta tccttattaa aagctccttg
     4201 acaagactat accttttatt ttatgatttt tacatagaaa acgttttctc tattttttgt
     4261 ttagaggtga tttttatgat ttgcaagaac aagaaaaaaa ttattgctat tattgctagt
     4321 ttctctttga tttttagtac tgcatgtact gtcttattaa caaataatgc taatgttgaa
     4381 gctgcaagct cttcttacaa acataatcat gctg
//
LOCUS       DACESX010000166         4217 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.168, whole genome shotgun sequence.
ACCESSION   DACESX010000166 DACESX010000000
VERSION     DACESX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.168"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            15..572
                     /locus_tag="IXF88_002522"
     CDS             15..572
                     /locus_tag="IXF88_002522"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1509219.1"
                     /translation="MGNEFVEDDIDILYVGRMAEPKNPIGAINVLENVVKKYSKHLRV
                     VMIGEGPLFDEVKMQIQKRNLTDVIELKGFKSNPYKYMKKSKVLLMPSLWEGFGLVSV
                     EAMLLETPVVAYNVGGLKDIIDETNGFLCGSEVELSNSLMKLLNSEELRKKMGENAAI
                     SAKKFSNMNRYQGVLEKIYVSAALK"
     gene            1101..1877
                     /locus_tag="IXF88_002523"
     CDS             1101..1877
                     /locus_tag="IXF88_002523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ1509220.1"
                     /translation="MAFQVYLQRVYILSTGVIIGHYAEMGLNRIAYAGLMGDFSFAGL
                     YLATGCLYAFLKYVDLKNWKLLFFIIIEVFLLGAILCVSARTGLVSLFVTMTLYYLFN
                     INRISVPHLLVLIGGIITTPYILVMLMAGRSGQSLLDSSGRLENYMSSLNAFSDKYLI
                     GYGLGLNNLYTLLGVAVPHNFFIQYLLQTGIIGTLIILSGFFKFTYEELKKSGCYKWL
                     FLMIVVGAMFIPDIVSSRFLYAIVVLCMISASERTIKKES"
     gene            1888..2349
                     /locus_tag="IXF88_002524"
     CDS             1888..2349
                     /locus_tag="IXF88_002524"
                     /inference="COORDINATES: protein motif:HMM:NF012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ1509221.1"
                     /translation="MRRKAEKWADKACRQYRNGQNKMSKISCYILRIIYGCEFYGQVD
                     FSSNAQLVHNGLGCVFHPKTKIGNNCKIYQNVTLGGNGKIINGKETVGAPTLEENVAV
                     FAGACVLGPINIGHDSIIGANAVVTKSVPPYSLVYGNPAIITELKFDYKFD"
     gene            2408..3865
                     /locus_tag="IXF88_002525"
     CDS             2408..3865
                     /locus_tag="IXF88_002525"
                     /inference="COORDINATES: protein motif:HMM:NF024832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAQ1509222.1"
                     /translation="MGNNIHGKTAKALKWSSLAEIIAKIITPISNMILARLLTPDAFG
                     VVATVTMVISFADMFSDSGFQKFLVQHEFDNEEHMFSSTNVAFTSNLGISLLLWLFIC
                     VFNNKIAHLVGNSGLGLVIIVAGVSLPITSFSSIQSAIFRRNFDYKILCYARIISALI
                     PLVITVPLAIIGLSYWSLVIGSIIGNLVTAAILTKLSEWKPKLFFSKKEFYEMFSFSA
                     WSLVESLGTWLSSYIGTFIVGGILSVYYLGIYKTTMTTVNGIFAIVTSATTTVLFSTL
                     SRLQNSKTEYDKTYLDFIKIVSIFIIPLGIGIYVYRELVTNILLGSQWKEAVPFVGIY
                     GLMSCFTLVLGQYASEYYRGLGKPKANVLMTILHLAVLIPVLIIYSRKGFIELAYGRS
                     FVKIEQIIVFWIILWFGFKFNPFKILKQVWKSCASALLMGVLAKIMLEFCTNYVMQLL
                     SVCLCVILYFFIYSCIFKGKTEIINMVRMFTGKGY"
     gene            3900..>4217
                     /locus_tag="IXF88_002526"
     CDS             3900..>4217
                     /locus_tag="IXF88_002526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1509223.1"
                     /translation="MKKVTILIPGMYYGGMERVAFITRQILLKNNYNVSFVTLFDNDA
                     DYQPNFEYLSLKCDIKPSKFGKVINIFKRVYKMKSVKKKLKTDITISFGQSCNLVNVL
                     SKRD"
BASE COUNT         1451 a          465 c          766 g         1535 t
ORIGIN      
        1 aaagaattat aaaaatgggg aatgaatttg ttgaagatga tattgatatt ttatatgtag
       61 gacgcatggc agaacctaaa aatccaatag gtgcaattaa tgttttagaa aatgtagtaa
      121 aaaaatattc aaaacattta cgtgtcgtga tgattggtga aggaccatta tttgatgaag
      181 ttaagatgca aatacaaaaa agaaacttaa cagatgttat tgagttaaag ggattcaaaa
      241 gtaatcccta taagtacatg aagaaatcta aagttctatt aatgccatct ctttgggaag
      301 ggtttggcct agtatcagta gaggcaatgt tgttagagac gcctgttgta gcttataacg
      361 taggaggatt gaaggatatt atcgatgaga caaatggatt tctgtgtggt agtgaagttg
      421 aattatctaa ttcactaatg aagctattaa attcagaaga acttagaaaa aaaatgggag
      481 aaaatgctgc tattagtgca aagaaatttt ctaatatgaa tcgttatcaa ggagtattag
      541 aaaaaattta tgtatctgca gctttaaaat aaaatatgaa ttataaaaat agagatcaat
      601 tagttttacg aaaaataata cccattagta ttttgtttca atcaggaatg attttttatt
      661 cactgatgcc ctcaatagca attgcagttg tttctttttt tatgtttttg tattatatgt
      721 ggtgcttaaa aaagtatttt tgtttagcag atagcttatt gttaacatca attcttttca
      781 ttccaacatc aactatatct atattaggaa ctagttattc tagcttacca ttgacgtggc
      841 ataatctaat aataataatt ttgatgttat atattattat agaaggaaca ttaaatcgtt
      901 catacttgat tattttattt ttatttattt gttttggaat atcgacgtct atgttcttac
      961 cttttttaat agatgcatta aagcaattaa tgactatttc gttgtttatc tgtagttttt
     1021 taataggaaa acgttttaaa atgtatagca aaaacttaac tatgattggg tggaatttct
     1081 atttcatagg agtagtatct atggcgtttc aggtatattt gcaacgagta tatattttat
     1141 ctactggtgt aataataggc cattatgcag aaatgggttt aaatagaatt gcatatgcag
     1201 gtttgatggg agattttagt tttgcagggt tatatcttgc tacaggctgt ctatatgctt
     1261 ttctaaaata tgtggattta aaaaattgga aattattatt ctttataata atagaagtat
     1321 ttttattagg tgcaatttta tgtgttagtg caagaacagg gttagtttcc ttgtttgtca
     1381 caatgacatt gtattatcta tttaatataa atagaattag tgttcctcat cttttagttc
     1441 taataggtgg aatcataact acaccttata tactagtaat gttaatggca ggtcgttctg
     1501 gacaaagtct tttggattct tcaggcagat tagaaaacta tatgtcctct ttaaatgctt
     1561 ttagtgataa atacttaata ggatatggat taggattaaa taatttatac acgctactag
     1621 gtgtagcagt accacataat ttttttattc aatatttatt acagacagga attattggta
     1681 cgctaattat tttaagtggc ttctttaaat ttacgtatga agaattaaag aaatcaggtt
     1741 gctataaatg gttatttttg atgatagtag tgggagcaat gtttataccg gatattgtat
     1801 cttcgcgttt tctttacgca atagttgttt tgtgtatgat ttctgcatca gaaagaacta
     1861 taaaaaaaga aagttgaggt agaaaaaatg agaagaaaag cagagaaatg ggcagataaa
     1921 gcttgtagac aatacaggaa tggtcaaaat aaaatgtcaa aaatatcctg ctatatttta
     1981 aggattattt atggatgtga attctacgga caggtagatt ttagctctaa tgctcaacta
     2041 gtccataatg gtcttggttg tgtattccac cctaaaacaa aaatcggtaa taattgtaaa
     2101 atatatcaaa atgtgacttt gggtggaaat ggcaaaataa taaatggaaa agaaactgtt
     2161 ggggcaccaa cgctagagga aaatgtggct gttttcgctg gtgcatgtgt tttaggacca
     2221 attaatatcg gacatgattc aatcataggt gcaaatgctg tagttacaaa aagtgttcca
     2281 ccatatagct tagtatatgg aaatccagca atcataacag agttgaaatt tgattataaa
     2341 tttgattaaa aagtaatgtt taatatgcga aaggttgcta tatttattta gcaaaatggg
     2401 gtaataaatg ggaaataata ttcatggaaa gacagcaaaa gctttaaaat ggtcatcctt
     2461 agctgaaata attgcaaaga ttataactcc aatttcgaat atgattttag ctagattatt
     2521 gactccagat gcttttggag tagtagccac tgtaactatg gttattagtt tcgctgatat
     2581 gtttagtgat tcaggatttc aaaaattttt agtacagcat gaatttgata atgaagaaca
     2641 catgttttca tcaacaaatg tagcttttac tagtaactta ggtatttctt tattattatg
     2701 gttatttatt tgtgtgttta ataataaaat agcgcactta gttggaaatt ctggacttgg
     2761 attagtaatt atagtagctg gagtttctct tccaattact tctttttcca gtatacaatc
     2821 ggcaattttt agacgaaatt ttgattacaa aattttgtgt tatgctagaa taatatctgc
     2881 attgatacct ttagttatta cagtaccgtt ggcgataatt ggactaagtt attggtcact
     2941 agtaattgga tctataatag gaaatttagt tacagctgca atattaacta agttatcaga
     3001 gtggaaacca aaactttttt tttcaaaaaa agaattttac gaaatgtttt cctttagtgc
     3061 gtggtcacta gtggaatcat taggtacatg gttgtcatct tatataggta cttttatcgt
     3121 aggaggcatt ttaagcgtat actatttggg aatatacaaa actacaatga caacggttaa
     3181 tggtattttt gcaattgtaa cttcagcaac aacaactgta ttattttcaa cattatctcg
     3241 tttacaaaat agtaaaacgg agtatgataa gacatattta gattttatta aaattgtttc
     3301 catttttatt attcctctgg gtataggtat atatgtatat agggaactag taacaaatat
     3361 tctactcggg agtcaatgga aagaggctgt accatttgtt gggatatatg gattaatgag
     3421 ctgttttact cttgtcttgg gacaatatgc ctctgagtac tatcgtggtt taggaaaacc
     3481 aaaagcaaat gttcttatga ctatcctaca tttagcagta ttaattccag ttctaataat
     3541 ctattcaagg aaagggttta tagaactagc atatggtaga tctttcgtta agatagagca
     3601 gattatagtc ttttggatta ttttatggtt tggttttaag tttaatccat ttaaaatttt
     3661 aaagcaggtt tggaaatctt gtgctagtgc attgctaatg ggagttctag caaaaattat
     3721 gttggaattc tgtactaatt atgttatgca attactttca gtgtgtcttt gtgtaattct
     3781 atattttttt atatatagtt gtatatttaa aggaaaaaca gaaataatta atatggttag
     3841 aatgtttact ggtaaaggat attaagagag atttttatgg caaggttgtg attaggaaaa
     3901 tgaaaaaagt aacaatttta attcctggaa tgtattatgg tggaatggaa agagtagctt
     3961 ttataacgcg tcaaatactt ttgaaaaata attataatgt atcatttgta actctttttg
     4021 ataacgatgc agattatcag ccaaattttg aatatttatc attaaaatgt gatataaagc
     4081 catcaaaatt tggtaaagta attaacattt ttaaaagagt ttataaaatg aaaagtgtca
     4141 aaaaaaaatt gaaaacggat ataactatat cttttggaca aagttgtaat ttagtaaatg
     4201 ttttatcaaa gagagat
//
LOCUS       DACESX010000167         4132 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.169, whole genome shotgun sequence.
ACCESSION   DACESX010000167 DACESX010000000
VERSION     DACESX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.169"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            158..1381
                     /locus_tag="IXF88_002527"
     CDS             158..1381
                     /locus_tag="IXF88_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ1509224.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFEPTESNLKMFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTIKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLENHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIAELRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TDIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKFMSRLQNKVYPLYDRLSKDRGFESVVSHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            1417..2184
                     /locus_tag="IXF88_002528"
     CDS             1417..2184
                     /locus_tag="IXF88_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1509225.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPNKLKKT
                     GEIVLPVKAIIKYMDVDQNLETELKNIVNTLQRQAVYEISNLDKEYKTYATVSAIDQI
                     HWDLEEQIITFNFSQEVIPLILSIKKKYPYFVFQDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            complement(2342..3301)
                     /locus_tag="IXF88_002529"
     CDS             complement(2342..3301)
                     /locus_tag="IXF88_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1509226.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            3440..3838
                     /locus_tag="IXF88_002530"
     CDS             3440..3838
                     /locus_tag="IXF88_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ1509227.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT         1441 a          629 c          704 g         1358 t
ORIGIN      
        1 tttattttgt aaaggttcta aaatagcgat ttctgctgtc ttatcaatga aatttgatta
       61 ataaaaataa aatgcgtttt actgagattg agttatgtta taaatttacg atacatttct
      121 tattgtatcg tatcgaaaac aggtctatac ttattcaatg gaatcgtacc agttccaagt
      181 ttttaagctc gtaccaagct tagaaagaag aggcaatcgt atgaaaataa cagaaaagaa
      241 tgtaaacccg aaatttgaac ctaccgaaag caatctaaaa atgtttgatt taactgttac
      301 tttatcgaat caacttgtgg atagcattgt tgaaatggat gctatgccac aaaaaattta
      361 tctttacgct ataggacatc ttgaccctaa aaatccgcct tttaataata ccatcaaagt
      421 atctaaaaag gctttatttg cgttgatgtc ttctagttca tcttcttcaa aacattcaag
      481 attaaaacaa gttattgaaa aattacatca gcaatctgtc attaaaattc aaagaactaa
      541 acctaacggt aagactgaat acaaagtcat ctcaccgcta gaaattactg aattcaatga
      601 tttttctgat gcagtatcta ttaaatttag tagttcaatt atgcctttgt taacgaactt
      661 agaaaatcat tttacacaat atacatttga tcaagtagtt aacttgacaa gcaaatatgc
      721 gatcagtttt tttcaattat tcactaaaga ttttaatctg tatgaagcaa atagagataa
      781 cttaacatgg agtacaaaat cacttgatag attaaaaaac cccgtttttt caattgcaga
      841 gttacgaaag aaattcagta ttgagaaaaa atataaagct tttcgagact ttgaaagacg
      901 tgtaattaaa gaaccgattg cccaactgaa taaatacact gattttaaaa ttgactatga
      961 gcttatcaag aagggtcata cttatacaga tataaagttc ttgcttacac gtaccatgtc
     1021 agtaccggta ccttataagc aagaacaaag agaccctgct tatttaaaag gtgcagaaga
     1081 taaaacagag aaaatgaatc aatcgtactt gaaagctatg aatagcaaat acactaaatt
     1141 actaatcaaa aactttttga tcagttctgc cgacttatgc gatctgaaat ttatgagtcg
     1201 gcttcagaat aaagtttatc cgctttatga tcgtttatcc aaagatagag gatttgaatc
     1261 agttgtcagt catctatctt acgttgctac tcataaggag gattacacaa taaaaaatac
     1321 tgttaaatat ctcgaaaaat caattacgag ttatttacct tatgtgaacc tttatgatta
     1381 acaactgaaa aacttgcgtg aaggaaggaa aagcttttga aaaaagatga actgacaaag
     1441 tacaacgagg agttcttagc aacaattgac atgaaaatgg atttaaagtc gtatgaactt
     1501 tatcaattgc tgttcagtta tatctctcct aacaaattaa agaaaacagg ggaaatagtt
     1561 ttacctgtta aagcaataat caaatacatg gatgttgatc aaaatcttga aacagaacta
     1621 aaaaatatag ttaacaccct tcagagacaa gcagtttatg aaatatcgaa cttagataaa
     1681 gaatataaga cttatgctac agtatctgct attgatcaaa ttcattggga cttagaagaa
     1741 caaattataa ctttcaattt ctcgcaagaa gtaattcccc taattctttc aatcaaaaag
     1801 aaatatccat attttgtttt tcaagattta gcaagctttc aaaaaaaata tagcattgtg
     1861 ttatacaaat atttggcgat gtattataaa aaatatctta tcgccaaaaa taaaggagat
     1921 tttgagaaag ctgaaatata taagaatccc ctgattgaaa tagttaaaat tcgtgagatt
     1981 acgaaaacag aaaatgagta caaaacaata tcgaattttg atagacgtgt tttaagggaa
     2041 tcagttgagg aaattaatga aaaatcaatg ctgaagattg attttgctaa agttagaaaa
     2101 ggcagaaatt acaattttgc tgtccagttt aatatttcta gtgagtttga tgaaaaacct
     2161 tatagagaaa aaaaattagg ttaatgcaaa atatatctta ctcgtttgaa gatgcttcag
     2221 acgtatgcta agatatgtag tgccttggga gcaaggtatt aatctccttt agaggttaat
     2281 tagcttgtga tcgcctatca atttttttgg taggcgattc tttttttgaa actttcgttt
     2341 gtcaaattaa gctagacaaa tcatctttac ctacgtaact caaaaatact tccaatggtg
     2401 tgcgatagtg taatgatttt ctaggaatat gatttcgttt tgatgccacc gattggataa
     2461 aggtttcgga taccttatta aaatccatag ctttatataa gccatcttta cgtaataagc
     2521 cattcgaatg ttcatttagt cctcgttgag aaggtgttcc tggatcagcg aaataaatcg
     2581 aaatatcatt ggtattacta atctttttcc aattcgagaa ctcttttcca caatcaaatg
     2641 tgatggattt aaacaagtgt ttcggtacag attgaaacca ttgattcagt ctattctcaa
     2701 tatctatagc ttgcctacca gcgggtttca aggtgatgat cactttagat aatcgctcaa
     2761 ctaacgtgat caccgcactt ttatgttttc caccgacaat ggtgtcacct tctaggtgac
     2821 caaactcatg attgaactgt atataatcag ttgttctatc ctgaatcgaa cggcgaaaag
     2881 cttgttttcc tcttttttct ttatgtccgt ttggttttct tttccctttc attggtaaat
     2941 cgtgagggtc aaaggtttta tctttaaaca tccgatatag tgtacgtacc gaacaagcaa
     3001 taggaaactc tgctcgacca ataaggacat ctggagtcca cccttgagcc accttttttt
     3061 gaatgtattc ttgttgccca tctggtaaca caatcggacg tcttccgcag ttacgtttgt
     3121 ttttcttata ttgttcatag tagcttattg cgcttccacc agatttaaga aaacgataga
     3181 cattataaat ggtttgtcga gaacgttgta ccagatgggc tacttttgaa acagagtgtg
     3241 attgattgaa ataagcttct atcattacga gttcagttgg tgtaagatgg gtataggtca
     3301 tttgtactca ctccttataa ttctttcgac ggaactattt tgagtgtagc acaaatgact
     3361 tttttagttg tctagcttaa ttttacaatc ggcgaactaa aaagcaaaaa ttatttttta
     3421 attagagagg tgcaatgcga tgaaatactt tttttgtgca gtattcgaat ggaaaggaaa
     3481 tgaatatgca gttaaattcc cagattttcc agacttatat acgcatggtg aaactttaga
     3541 agatgctttt gagatggcgc aagaagtatt ggaaggatac ttggtgcttt tagagttgca
     3601 agaaaaagaa attccatcgc ctacgcctgt tcaagagtta aatgcaagaa tcaagcgatc
     3661 tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa gttgctaaag ctcgaaacaa
     3721 gcaaaattac gatcgaaaat cagtgactgt ggaacactat ttaaatgttt tagcggctga
     3781 agagaatatt aatctgtcga aagctttgaa tgatttgttg agagaaaagt tgatttgatg
     3841 tctctcagat gatcatatta agcaaaaaca gcttgtttta ggacgtatgg atttaagaaa
     3901 gacaaattat caaagaatat attaaaagct ctcagaagcg ttctgagagc ttttaaattg
     3961 ctatgtatta tactgcatct gctcaaattg attttctttt catgcttacc atgtatctag
     4021 ttttccatca agtaattttt agatatgtat tgaggaataa atagcaaatt tgcttgatat
     4081 aagctcgaat taggtgcttt gtaatgtgtt tggataaatt atacaagaaa at
//
LOCUS       DACESX010000168         3904 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.170, whole genome shotgun sequence.
ACCESSION   DACESX010000168 DACESX010000000
VERSION     DACESX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.170"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            26..994
                     /locus_tag="IXF88_002531"
     CDS             26..994
                     /locus_tag="IXF88_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1509228.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1005..1820
                     /locus_tag="IXF88_002532"
     CDS             1005..1820
                     /locus_tag="IXF88_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ1509229.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            1888..2598
                     /locus_tag="IXF88_002533"
     CDS             1888..2598
                     /locus_tag="IXF88_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1509230.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            2634..3875
                     /locus_tag="IXF88_002534"
     CDS             2634..3875
                     /locus_tag="IXF88_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ1509231.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKNESSPQSCVKVQPHQKH
                     NVLRCEPNCHAFLSEERGQEQ"
BASE COUNT         1283 a          589 c          877 g         1155 t
ORIGIN      
        1 ttaaaatttt aaggaggaaa tgttgatgga tgtagcaaaa acggttaacg caattgtaga
       61 aaaaaatgat ggagagattt ctggaattta tttggtggct tgtggcggtt cgctggtaga
      121 tatgtatgtc tcagattatt ttcttaaagc cgaagcatcg aagtgtatta caggcttata
      181 tacagcgaac gaatttaacc atgtaacccc aaagcaatta gatgataaat ctgttgtcat
      241 tctatgttct catggaggaa atacagcaga aacggtaaaa gcaggagaag tcgctcaagc
      301 tagaggagct gtgactgttg gtctgactca taataaaact gcggatttat taaaggtttc
      361 tgatcatagt ttcctttacg aatggggaga tgatgcaaaa atagtcaata atcctatggc
      421 catcattctt gatttaactg tatctttggt caatgttacc gaaacatttg aacattacgg
      481 taaattcaga gaaggcatga aggttatcga taccgtaatt gaaaatggca ttaagcaggt
      541 gcaggatcgt attaaagtgt ttgctgataa gtacaaagat gaaacaatgt tttatatctt
      601 aggtagtgga ccatcatatg gacatgctta tggtttttca atttgctctt tgatggaaat
      661 gcaatggctt gattcaactt ctgttcattc tggagaattc ttccatggac cttttgaagt
      721 aacagacaaa gatacgaatt ttattctctt gatgagcatt ggacgcactc gtgctttaga
      781 tgaacgggca aaacgattcc ttgataaata tgcagaaaga gtagaaatcg ttgatgcaaa
      841 ggaactagga ttggatatga tatcagatga agtttctgaa ttcttcaatc ccattttatt
      901 ctacagtatt ctttcagaat atcgttcagc tttagcagat gcacgcaatc atgatttaga
      961 tgttcgtcgc tatatgggga aagtggagta ctaggaggat taaaatgaag gtattaggct
     1021 taggtgataa cgtcgtcgat aaatatgaaa atctgggaat tatgtatcct ggaggaaatt
     1081 cattgaattt tgcagttttt gcaaaaaaac tgggacatga aagtagcttt atgggagttt
     1141 ttggctcaga tgaggaggca gctcatgttt tatcagcaat aaaaaaaata ggactggaca
     1201 acagtcactc tcgctttgaa aaaggcgaga atggctgtgc cagagtgaag attgatgagg
     1261 gcgaccggat tttccttggc agcaattcgg gaggtgttac tcgtgaatac ccgattcagc
     1321 ttactgaaga agacagagaa tatttaaacc agtttgaatt gattcatatg ggattatata
     1381 gccatgtcaa tcatctatta gaagagttac aaaatgtttc tggaaagatt tcttatgatt
     1441 tttctgacga ctttacggaa gaagaggttc agcgggcaat tgataaagtt gattttggct
     1501 tcttttctat agaagacttt tccgatgaag aagtaaagca gtttttgatg aagcattttt
     1561 gtgagcgaaa tgatgtattg attgcaaccc gaggagaaaa aacggctatt ggttatgatg
     1621 gagcaaagtt ttatgaagtg aaccccgtgc tagaagaacc agttgataca atggcagcgg
     1681 gagattcttt cttaacagct ttcctacttt attttctcga aggggacgat atagtcaccg
     1741 cgatgaaaaa ggggaatgaa tttgctggga agtcttgtct ggttgacggt tcgttcggct
     1801 ttggtattca ttacgattga gcttgtttta aattcctgca tttgataacc taaagaaaga
     1861 aatcccggta aagtggaggt taaaaaaatg ctggatacaa atgcacaatt accattgtac
     1921 ttacaattaa aagaaatatt aaaaaataaa atcaatacag gggagtatcc cgagaatacg
     1981 aaaattcccg ctgaatcaga gctgatagaa agttatgagg tcagccgaat cacagtgaga
     2041 aaagcagtag aagaactggt agaagaaggg tacctactta aaaaacaagg gaaaggaact
     2101 tttgtcaacc ggcataaagt ttttcgtaaa attgaatatg ttatgggttt cagtgagagt
     2161 tgtaaatcta atggttttac accgactagc attgttttgg aacgcaagat cattccagca
     2221 actgaaggga tggcaaaaaa actcgatatc gctcaaggtg atgatgtttt atatatccag
     2281 agaaagcgaa tggcggataa catccccatt atgttagaaa acaattattt ttcttatgaa
     2341 agatatagtt tcttaatgga ggaagagctg actgattctt tgtatgaagt acttaaagtc
     2401 caaggtacct tagccatcaa tcctggggaa actgcacttg aaattgtcac agctgatgac
     2461 caaattgcca aaataatgga agtagctgtt gggacgccct ttttctttat ggatacttta
     2521 atcaacgatc aaaacaacat accaattcat gtaggtaatc aatattacct gggagaagta
     2581 tatcgttttt acttatgatt tcctgttttg atttcaagga gtagatttta tttatgaagc
     2641 aagtattgcg gtcaaaaatg atttttgatg gagagcagcc acctttcaaa ggttatattg
     2701 taatcaatga agggatcatt gaaaaggtgg aaaacaactg gaattacagt gatctattaa
     2761 atgaaaacac ggaattaatt gcttatgatg atcaattcgt tatgccagca attcatgata
     2821 atcatgtatt tttcagtggt tatttggcta tgaatgctgg catagattta agcgatacat
     2881 cttcagctaa cgaggctgta gagcgtatta aagctgtaat gaaccataaa aaagcagatg
     2941 aaccaattta tgctcatggc tgggatgctg ccctgtggaa aactgaccca tcagaagagc
     3001 tattgaatga aataaaccac tttggtccaa tcacagcgat cgacaaaaat cgcagccatt
     3061 gttggatgaa tgctattgct aagcagactt atggttttac agaaaaagaa accagtgctg
     3121 aaagtcgagt attattaatt aaggaaatgc tagcaaacaa aaagctttta gcagaggttt
     3181 acgctgaatt tgaagcatta ctgctttcaa aaggtgttat atcaatgaaa gaaatcgttt
     3241 ttgatgacgc agaatttctt tctaatatac ctcagaggaa aatacttagt aacctatatg
     3301 tgcaggcagt ccaagaacct ctacagcatg aacttctata tgagtataag gaaaaagttt
     3361 tccctgaaaa ggtccgcttt ggtggagtga agatcatggt agatggtgtt gttgcggata
     3421 gaagtggaga tatttacggt gtttatcagt caggagtagc ttcacccgag attgactatg
     3481 aggcaattaa agcagaagtt gctgcattca atgaagtaga gattccttgc tgtttgacaa
     3541 cggaaggaga taaagcgggt tataaagctg cgtcaattct tgtcgaattt ggaaagcggt
     3601 tacctgaagg cgtttttaat tcgattagtg atttggaaat ggttactcaa aagacagcag
     3661 aaaaaatgaa cgaaggacaa gtagtagcag aggtatatcc acaaattctc ggcttgaatc
     3721 aaacgttaga agaggcttac atgtcaactg ttataaaaaa tgagtcaagt ccacaatctt
     3781 gtgtaaaagt tcaacctcat caaaagcaca atgttttgcg ttgtgaacct aactgccatg
     3841 cctttctttc tgaggagaga ggtcaggagc agtagcgcag cgactcttga tgtctctcct
     3901 caga
//
LOCUS       DACESX010000169         3854 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.171, whole genome shotgun sequence.
ACCESSION   DACESX010000169 DACESX010000000
VERSION     DACESX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.171"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            81..1097
                     /locus_tag="IXF88_002535"
     CDS             81..1097
                     /locus_tag="IXF88_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1509232.1"
                     /translation="MFNFDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGG
                     VGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELV
                     EYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRN
                     EIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHKDTSYHILTGAGDSFAEAY
                     YYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIITEGEDDSRPLTERVKNFVP
                     KFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERISAFLEEERNHPLTTRRYYK
                     QMEY"
     gene            1205..2074
                     /locus_tag="IXF88_002536"
     CDS             1205..2074
                     /locus_tag="IXF88_002536"
                     /inference="COORDINATES: protein motif:HMM:NF012585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509233.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            2089..2793
                     /locus_tag="IXF88_002537"
     CDS             2089..2793
                     /locus_tag="IXF88_002537"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012610.1,HMM:NF019322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1509234.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
BASE COUNT         1290 a          673 c          666 g         1225 t
ORIGIN      
        1 tatttgacac tttttattac ctgtggtatt ttatttatgc aaacaaaata ttaccattgg
       61 taatcaaagg agagattttt atgtttaatt ttgatcaaga aagattttta aagactcaag
      121 ctggtgccgt tgcaacaaat gaacaaattt ccaatatttt agaatccatc gcatctgact
      181 tagaagaaat ttactttatt gggtctggag gagttggtat tctttttgat tcaacaatcg
      241 aattcgcgag aactcgtagc actttccctt ttcatcgtgt tatcgctgcc gaatttatga
      301 ttcatcccaa taaaacattt tctaacaaat ctttggttgt tatcccatct ttatctggta
      361 caacaaaaga aacacttgag ttagtcgaat actgtaatga aatgaacgtc aaaacattag
      421 ctttggtagg aagtcctaaa acgcccctag aaaaagctgc aacatattct atctataacg
      481 acgttttaga tgatacatct tcagaaaatt attaccttca aggactgctg attgctctct
      541 tcttaatgaa acaaagaaat gaaatcgata tcaatcaata caatacctat attgaggatt
      601 taaaaaaaat acctgaagcc ttagtgcacg ctaaagagca agcggaacca atggcaaaat
      661 cttttgcaaa aaaacacaaa gatacttcat atcatatttt aaccggtgct ggtgatagct
      721 ttgcggaagc atattactat ggcatgtgta ttttagaaga gatgcaatgg attaagactc
      781 gtcctgttca tgcagcagac ttcttccatg gaactcttga attaattgaa gcgggtgtaa
      841 gtgtcatcat tacagaagga gaagatgatt ctcgacctct cacagaacgc gtgaagaatt
      901 tcgttcctaa gtttacagat gttcacgtaa cggttgatac aaaggactat gacttacctg
      961 gcatttcgga agacacgcga aaaattattt ccccagtcgt cttagctact gtctttgaac
     1021 gaattagtgc ttttctagaa gaagaacgta accacccttt aactactcgc cgttattata
     1081 agcaaatgga atactaatca taaaaatatg aaaaaggtga ccgcgactcc attgcccatt
     1141 tgattgcttc attttctcta atgggagaat agaatcgcag tcaccttttt taggaggatc
     1201 taaaatgaaa atcgcagtta tctgtagtgg tggcgactct cccggaatga attcagcttt
     1261 attagcaata aaaaaaacaa ttgatagcga accagattcg gaagtagtct tttttaagaa
     1321 tggttatgcc ggaatattta aggaatgtat tgcctcagtt tctctaaaag aagttgaggt
     1381 attcaaagct ggaacactta ttggaacagc cagatttgag aaaatgagag atctcgaaag
     1441 tcggaaaatt attattgaaa acctaaataa gatggagatc gacaacttaa ttgttattgg
     1501 aggtaacggt tccttgaaag gagctgaact attgaaaaat gaatcagaat tacaagtatc
     1561 tgtcatccca gggactattg ataacgattt ccccaatacc gattatacac ttggcttcga
     1621 tagcgcctta aacactataa tccgatcaat cgatgattta acttctactg ctaatgctca
     1681 tgatcgtatt attataatgg aagtcatggg ccgaggctgc tttgctcttc cagagtacgc
     1741 ttccattgca aataatactt attgggttga gagagacgac aaggaatact tacacttaat
     1801 atgtaaaaaa tttgagtcgg agttctactc gcctatattg cttttaccag aaggtatatc
     1861 taacaaacaa aaaatcattg aatttattac caaaaattta ccaaaagaaa ccaaatactt
     1921 aaatcttgcg tatcttcagc gtggatcatc tccaactttt tatgaccgat atattggcta
     1981 tttgtggggt caattagcgg cgaaggaatt aattgaagga aaatctggga tattaagtca
     2041 aaaaaatggt aatataatgt taataccttg gtaaaaggat ggattactat gatttttgac
     2101 aaagtaaaag aacaaattcg aaaagtgtat atcaccactg atcggaaatc tgaattattg
     2161 ccttcggaaa gagatttggc ccaggaattt tcggtgagtc gtccaacaat tcggaaagct
     2221 ttagcttctt tagaagacga aggaattatt tctaaaaatg aaggtcgcgg gtatctagtg
     2281 aaaccagctt cagaaaaata tgttgaccac gaactcgact cttttatcgg attcttccaa
     2341 gatgcggttc agcaaggaaa gaaaatcagt tctaagatta tccaacaaac catcatacct
     2401 gctgattcaa caattagtga atgtttggag attgagcaga atactttagt gcttgtcttg
     2461 gaaagaattc gatacatcga tgactatcca acttgtattg caaaaagttg cattccccta
     2521 tctgttttac ctgaaattgt tgaacatgat tttgccaaag aatccctgtt ctcagtatta
     2581 gaaagtaggg gaattagatt agcttatgcc aaacgctcaa ttgaagttac accgccaaca
     2641 gatttagaaa acctttatct tgatttagaa aaaaatgagc cagtctttgt ctttacaagt
     2701 attggatttg ctaaaaatca gaaaccgttt gaatatgaaa tatctaagta ccctgcttat
     2761 aagacaaagt ttgaaagtac tgtttccaaa taatttttaa atatgcttaa atgtataaag
     2821 agcctcgcgg ataacagata aagtgtcttt ccgcaaggct ctttttctac ttcctcttta
     2881 tcttaatccg ctaagtagcc tacagtccaa acattatcat ctaattaagg tgtgtggagg
     2941 tactcctcta ccctaagtca aggatagtcg acatagttgt taataagtct tcaaagcaag
     3001 cctgaacctt tttcattagg tgtgcttact tcacatatga cgctatcaaa aatgatagca
     3061 cccgttgaat caaccaattt tctctaattc atatccccat tcaaacaact atattttaaa
     3121 aactttttta atctttgatt atgtgtagca aatttgtcta atttttattt ctttgcaaaa
     3181 tttttccaaa tttaccagat aaaagtaatt catattcatg attgagtcct ttaccaataa
     3241 aacctatttc gcaaaaatta tttgtaagca ttaaggattt tctgctgact ttcactggaa
     3301 ggataaccca agaattctga ccaaagtgaa agattaagaa ccatctttgc cagtctatct
     3361 atgtgctatt gtaactgtca acatcatgcg ggcagttatt attatttatt tgaataatat
     3421 ttttattata aatcagtttt ttagattgtg cataatcact aattgctgtg attaattaca
     3481 gaatatggct ctctgggaaa atgtgcgcgt catgtattca attttaaatt ctaatttgta
     3541 caaaatgaat cgtcacgact cgatctgacg caactccaaa tgggggcgcc gacctgtacc
     3601 aagtacagtg gcatgttctt taaacatttg aagcaaaatc tgtccatcaa aaacctgtat
     3661 tttcaaagtg aacaaggtgc gctaaatcag gaaatactta cactgatcat ttcttaattt
     3721 acctacttaa ccaaaattta aatcaatatg aaagcaacac ttttcaaact aaagaggtct
     3781 ttttaccgct tgatgtttgc ttcgataaaa aagttgatgt cggattagtt ttcattttaa
     3841 taaaacgtac tatt
//
LOCUS       DACESX010000170         3814 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.172, whole genome shotgun sequence.
ACCESSION   DACESX010000170 DACESX010000000
VERSION     DACESX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.172"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..1410)
                     /locus_tag="IXF88_002538"
     CDS             complement(<1..1410)
                     /locus_tag="IXF88_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HAQ1509235.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQ
                     MLAFSTVTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRL
                     TDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATW
                     LWKGKEPLFFCSKIRNEVDK"
     gene            complement(1382..2581)
                     /locus_tag="IXF88_002539"
     CDS             complement(1382..2581)
                     /locus_tag="IXF88_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1509236.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(2711..>3814)
                     /locus_tag="IXF88_002540"
     CDS             complement(2711..>3814)
                     /locus_tag="IXF88_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1509237.1"
                     /translation="VKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVND
                     STKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNE
                     KGADETVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDAT
                     FFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGSLI
                     FAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQ
                     YTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIY
                     VVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT         1135 a          755 c          617 g         1307 t
ORIGIN      
        1 tttatctact tcgtttcgaa tttttgaaca aaaaaataaa ggctccttgc ctttccatag
       61 ccaggtggct aatttcttta aattcattgc agcaaacact agcatcgtat gcgtcgcgac
      121 tttttccaag cctcgatact tggtccaacg catcccatgc ttttcttttg catccgcaaa
      181 tattcgttca atcgtttgtt tccgtttttt gtaaattgat ttattcagat ctgttaggcg
      241 aagatgttcc acttcatcca tcagatctcc ccaaacatgt ctcgtgatga ctcgttgatg
      301 atttttactt atggtacatt ggtttagcag aggacagttc acacattctt ttggattgga
      361 tttgtattct cggtaccctt ctcgagtaac cgttgaaaaa gcgagcattt gatcttgcgg
      421 acaaatataa caatcaaaat actcgtcata gagaaactca ttttttgata atagcccctt
      481 tttccctttg ggccttgaat aaggcaaaca aggtcgaagg ttcaaatgag tcaaaaagtg
      541 aacaaactta ggtgtcttat acccagcatc agcgacaaca caattgattt caggaaaaag
      601 tctcttcgat tgtttgacga gctgaaaggc gacttgactg tcatgaatat ttccaggagt
      661 gatgattgtg ctaagaataa atccattgtc gtcgcatgaa gtatgggcag aataagcaaa
      721 ttgtttttct cgttcgcctt tgacgtagta gccgctttcc ggatcagctt tacttatttt
      781 tctttctttc agcttcactt ccttttctaa gtccaaagta aaagggcgtt ttccagcctc
      841 tattcgttga cgattgatct catcttctaa ttcatcctga taggctttcg cttcatcatg
      901 ggtcatttct ttcgtaaact tatgtttatt ggcgttagct ttgatgtggg tagaatccag
      961 atacaaatta tcctctgtta caaaacctgc tttaactgct tgttctaaaa tataggcaaa
     1021 aatatcctca aacaccgtcg tttctctaaa tcggcgaaca taattctttc caaaagttga
     1081 gaaatggggg attttctcct cgaaggagta gcctaaaaac cagcggtaag cgacattggt
     1141 atcgacttcc ttaatggttt gtcgcatgga acggataccg aataaatact gaataaacac
     1201 caatttgatc agaatcactg gatcgatact tgggcgacct aaatcggagt aagtggcttc
     1261 gacgattgga taaataaatt caaaatcaag cgcggcatca atctttcgaa cgagatgatc
     1321 ttgaggaact agatcatcaa tcgtaagcat cgcaaattga tttctaccat tcgtattgtt
     1381 tttagacatc atagctatca ctccttgcat tcgttttcat aaatctatta taaaagaaaa
     1441 gactgtagac atcatccata aaatggactt tgtctacagt ctgcgcattc tcattagaga
     1501 acactccctt ttttgtgact tttctgaagc tagagtgaat actttcaact gcattagtag
     1561 tgtacatgat ttttctaatg gcgctgccat aatcaaagag ttgttcaaca tgagaaaagt
     1621 ttcgtttgca tacatctact gcaccagaat aatgagacca ccgattttga aaactgtcga
     1681 aggcagcgtg tgcagcgttt aaagaagaag cactgtagaa ttttttcatg tctcgacaga
     1741 cttccttata atccttactt gggaggtatc gtaacgcatt tcgaacaaga tgaacaatac
     1801 agcgctgcac aatgacagaa ggaaaaattg cttttgcgcc ttctttgaga ccggaaacac
     1861 cgtccatcga aatgaaaaaa acatcttcta caccacgtac tttcagttca tcaaatactt
     1921 gcatccagcg atttttagat tctgtttgat tgagccatag gcctaaaatc tccttgtttc
     1981 ctttaagatc atagccaaga atggtgtaca cagcatattc tttagcttca tagttttcgc
     2041 gtaaagtgac gtacatacaa tcaacgaata gaaatgcata acacttggct aatggccgag
     2101 cttgccattc ttccaattca ggaaggacgg catccgtgat atcagaaatc atttcgtggg
     2161 aaatatcaaa gccgtaaata tcttcaacgg ttgcggcgat atcgcattga ctcattcctc
     2221 gtgcatacat ggaaagaacc ttaccttcga tgtcggagac atctcgtttt ctattaggaa
     2281 tcaactcagg ttcaaaagaa gcctcccggt ctctgggaac atcaatgttt actttaccaa
     2341 agctcgtttt aagcgtctta ttgccataac cgtttcgtcg attgtcatgt ttctttggct
     2401 ctttagaatg ggcgtcatac cctaaatggt tattcaattc tccttgaagc attttttcaa
     2461 ataagggacc aaacacatct tttaaaacat cttgcatatc ctctacagat tcgggttgat
     2521 aagtattcaa aatggattca gctaacttct cagcatcagg atttcttttc tttctagcca
     2581 tcgtgtaatc acctcttgat cttattttag aaaaataaaa accgtgaagc aaccacccgc
     2641 ctaggattga tggttactta cacggtttat attacactct catttttggt gctaaaaagc
     2701 accgaaaatt ttaattattg tcaattaata attcttttgg attccttctt aatatattga
     2761 atgaagaaat aatcaatgat acaagaagta ctaaactcat aaatataact acataaatca
     2821 tgaatttagg taatacattg atatctaaac ttgataatgt cttgttaaat ccttcagctt
     2881 ctgccccacc acctaattgg ctagatgcag attgtttagc tatttgttta gctatatcac
     2941 cagtaacctt attcaatata ttattcccaa gcttgtcagc tgtatattga gctaagaaat
     3001 aggaaccaac gaatgcgggg attgatataa ataccatctc aattaggaat tgaccaaaaa
     3061 tttctaattt tgatatacct aatgatagta atactgctat ttctttcttt ctagcattca
     3121 tccataagaa taataataat gaaactacaa ctcctgcaaa tattaatgag ccaaaaaata
     3181 atttatttga aattgagtaa atacctgata tagattgttg taatgcaggg taatttgatg
     3241 aacttttaat caaattatat tctctccaat ttatatctaa tttcccaaga tcttttatta
     3301 cactatcaag attcttatca ccttttacaa agaatgttgc atcttggtat acagctgtat
     3361 cttcggtatt accataaact ttagcagctg tatgaatatc agtgattaat gtattttcgt
     3421 atagttcttg tgctgcactt actccaccgc tattatgccc atcgaataga cctttaattt
     3481 ctacttctac tgtttcatct gcacctttct cattatcagc atcaaataaa ttagatttta
     3541 tttttatctt atctccaact ttaaggttat ttttttcagc taaatcttta tgcattaaga
     3601 ttttattttt atcttcattt tctaaatgct ttccttctac taatttatat gcttctgata
     3661 caaattttgt ttcttttgtt gagtcgttaa ccccagttaa cataactgtt cttttaaaat
     3721 tctttgctct ttcaggtgat tgattcgcaa gagtctcttg agtttcaata atatcataat
     3781 caactaaatc tgcaacactg tttattcttt ttac
//
LOCUS       DACESX010000171         3780 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.174, whole genome shotgun sequence.
ACCESSION   DACESX010000171 DACESX010000000
VERSION     DACESX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.174"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(94..975)
                     /locus_tag="IXF88_002541"
     CDS             complement(94..975)
                     /locus_tag="IXF88_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1509238.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(1051..2340)
                     /locus_tag="IXF88_002542"
     CDS             complement(1051..2340)
                     /locus_tag="IXF88_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1509239.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(2568..3212)
                     /locus_tag="IXF88_002543"
                     /pseudo
     CDS             complement(2568..3212)
                     /locus_tag="IXF88_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         1134 a          869 c          560 g         1217 t
ORIGIN      
        1 ttcaatggct taaaagaatt ggccataata aacaataatg gatagataat aatgattatg
       61 atgaacataa ataagagatg tggcaaccag cgtttaattt tcatagtaaa cccccttttc
      121 cacaaattta aatattaccc ctaagaaaac agcaaatatt aataaagtta aaactgaaat
      181 tgctgaagat tctccgactt tatacatttc aaaagccttt tgataagcat aatagatcac
      241 gtttgcacta ccattattcg gtccaccacg agtgatgatc ttaatcggag taaacacata
      301 ctgcagacct tgaacaatgg tcatcaccaa taaatatagt cctgtcgaac tactcatagg
      361 caacacaatt gtaaaaaaaa tgcgccagat gggtacacga tccaacttag ctgcttcgat
      421 catctcttga tcaattccca caattgctgc aaaaatgaca atgaaattat aaccaaaaac
      481 tttccaagca gtaatacctg caatcacagc aattacccaa ccttgtaaat tcatccacat
      541 aggcagtctt aaaccaaaat ttcccaacaa gacagcaatt ggtcccgata ccggatttaa
      601 tatccatgta aaaaccatcc ccgccactac caaagaaatc acagctggaa taaaaaataa
      661 tggtttatac aaattctgcc ctttttctat aactattcct aataaaaagg atgtaatata
      721 tgggaacaca cagctcagca agacaaatag aacggtatag gacaaagtat tcttcaacac
      781 tttatgaaag actggatcag tcagcacctt tacatagttg gcaaagccaa caaattcttt
      841 agtgggggcc accatattcc agtcaaaaaa actcaaatag atcgtataaa tcaatggata
      901 aaaaacaaac acgcccaaca aaataattgc tggcgccaga tacaaataac ctacatagcg
      961 cttcatttcc gacagcctcc tcatccttat gtcaaaagca gacgaccttg tttttccttt
     1021 cttacaggcg ctgtcgtctg cttgctttgc ttatttgagt aactcattaa tttccttttg
     1081 agtagctttc atcgtttcat tgacggaatt acctgataaa attttatccc gcatatccaa
     1141 caatttttgt tcagcttcta atccagcatc tccaggaaat gaactccaac tgaccatctt
     1201 atccatctga tcaattgctg gtatcatcat ctgattttca gctaagaaag cttttaatcc
     1261 attttgggcg tctgcaacat ctttacgtgg tggtaaataa ccagtacctt ctgtccattt
     1321 agctaaagat tcaacactat aaagatattt taagaaatcc caagttgctt cctttttctt
     1381 atccgtttgt gatgtaacag ctaacattgc gccaccagca ggtagtttaa cttttttccc
     1441 ttcccaactt ggtgactcaa ctgctttagc agtaaaagta ctattttttt gaatgttgtc
     1501 cctttgagca atagtggtat agaccatcgc gacgttacca tcaataaagc tttgcacacc
     1561 ttgttcccac gtagtgtgta atgccgtctg gtcttttacc atcctttgat ataattcata
     1621 agcagcgata ccatctttag aagcaaaggt agctttgcca tcttttatta cacttgcccc
     1681 attactttct aaaattgctt gttgtgccca attatcagca ggttcttgaa tatacacgcc
     1741 atactttcca gttttttctt ttattatctt agaaaaagtt tgaacttctt cccaagtttt
     1801 tggaccattt tcatccaagc ccgctgcttt taatatatct tgatttaaat ataaaaccgg
     1861 attactaatg gagtaaggaa tacccacttg tttaccatct ttttcagaca gtgccatgat
     1921 attgtctaaa aaatgatctt taaaataatc agcattatcc ggatccactt cttctactaa
     1981 agattgagga ttagtatatt tgaaattttc tgcaaaataa tctttaaaag cccatcccac
     2041 ttgtactacg tccggtattt ctttggaggc aacagaagct tgtaaatttt gcatcaagcc
     2101 tttatacata tcaggattat attttgctac aaccttcact ttgtcttgag attgattgta
     2161 tttatctact aattcattca ctgtttttcc acctagggta tccgcattaa catgccaata
     2221 ctctatcgtt acacgaccct ccgatttttt tgttgtcttg gtattcccac taccacaagc
     2281 tgccaaagag atgacactta aaacagacac aacccctata acaagatttt tctttaacat
     2341 aacattaact tcctcctaat ttctttatgg ccatttttct aatcaagaaa tattatgaac
     2401 taccaatgta aatttctagt taattttctg taaataattc gaaatttgtg catttcgggg
     2461 tacagggtca ctggacatat ccatcaagct aagaataaag cccccatatg gtcatgtatc
     2521 ccatctccaa atctcttaac tcctctgttt tgattataaa atagtttatt ctgtgctcct
     2581 ttcttccata aataggccca tgcacaacgt gaggttatat tataaacacc acagcgcgca
     2641 aggtactcaa tcgtttctag ttcattgact gtccatacag aaaatgtcaa tcctagctgc
     2701 tgccccactt cacgggccaa cttagttaac catccttcat gaatattgac gatttgaacg
     2761 ctttgatagg ctaatttctg taaggtatcg atcaaatctg cttcggtaat atccccaagt
     2821 tttttccctt cgaactgatt ttcaatatta taaaatatat gctttcccca aatcgcagcc
     2881 caagataatt gtacatagcc actaagaaca atttgtggcc agatttgata ggtttgtgcc
     2941 agcgaaaaaa cggccatttc caatccctct tcttttaaat cacagttaat ggtcatggtt
     3001 gccttctcat tggccactgc ttgaaacacc tgctcaaggg ttaccgaatc tgggacaact
     3061 tcttacctgt catgggctag tattagcgta ccatctgttt gctgacgcac atctacttca
     3121 aaacaaagct ggcgatccaa acaaaagcga agaaatgccc agctattctc ctccgtattg
     3181 tcactaccag agtgtgcagt gatcatggtc attttgaccc ctcctttaat tccgtgattt
     3241 tctcccacac gtccactata ccgatgaagc gttaatttct tgttaatttc ttgtaaattc
     3301 cctgcgtttt ttcagtaaac tagttgtaaa tcttgcatgc tccttcctac gaactcatga
     3361 atcgtcgatt ttgcccttct agctagtttc accaattaag tagttctaca ttccccgctt
     3421 ttaacctact acaccttttt ttgttttcag ctattccttc ttcttgctga aagtccttga
     3481 ggttctttct tacaggatac catcaaggtg ttcaatggct tattcccatt gaatgaccga
     3541 gacttactat cagatttctg ctcgttgaac caagtctttg atacaggatt tcttccgtca
     3601 tcttctcaca aataccacct tgcgtttctc tagcgattgg tcgatgtgca ccctcccagt
     3661 ggactgtcac tacccctttt tgctatgcct agcccacaaa aaaagctgtc ctcacagaca
     3721 gccagactgt agacaaaagc tgttccaaac tcgtaactcg agcttggaac agctttttat
//
LOCUS       DACESX010000172         3661 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.176, whole genome shotgun sequence.
ACCESSION   DACESX010000172 DACESX010000000
VERSION     DACESX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.176"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            82..2916
                     /locus_tag="IXF88_002544"
     CDS             82..2916
                     /locus_tag="IXF88_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1509240.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3107..3598
                     /locus_tag="IXF88_002545"
     CDS             3107..3598
                     /locus_tag="IXF88_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1509241.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
BASE COUNT         1245 a          615 c          797 g         1004 t
ORIGIN      
        1 tagttgacga aacagaaata aatacatagt attatgtttt gaagcctaag tacaatagtc
       61 tatagaaaag gagcgatacc attggcaaaa cgcattgacc gaatttatat gtatgtcaaa
      121 gaaaaaaccg ctcatttaac aaaggcagaa tatgatcaag gagtaacaac acaggaaatt
      181 gctgaagtgt tagggattca gcgaacaaat agcagtaaag atctgaatca gctagtgcgc
      241 gaaggaaaac tgataaagac agacggacga ccagtacgtt acatatatca aacactcgtt
      301 acgcacgtaa agccagttac aaaacatgta gtcagttaca aagaaacgcc ggtctctgaa
      361 gaaaaaaata cactccaaat agatactaag gatatctttg caaaaatcat tggagctaac
      421 ggcagcatga aaaattcagt ggaacaggca aaagctgcga ttttgtatcc gccaagagga
      481 ttaaattgtt tgattacagg acctaccgga tcaggaaaga cttattttgc acacgcgatg
      541 tatcattttg caaaagcgca tcatgtaata gatgaggaaa cggaactgat tgttttcaat
      601 tgtgctgatt atgccaataa ccctgagctt ttgatgagtc atttatttgg gtacgcaaaa
      661 ggtgctttta caggcgcaga tgaagaaaaa atggggatta tcgatcaagc ggatggcggt
      721 atgctgtttt tggatgagat tcaccggttg ccgcctgaag gtcaagaaat gatcttttac
      781 tttatggatc atggaactta cagtcgtctt ggggaaacaa cgaaatccca tgaagcaaat
      841 gtacggatcg taggtgcgac cacagaagac ccaggttctt ctctattaga aacttttgtt
      901 cggcgtatcc caatcaatat caaacttccg gcttttgaaa aacgtcctgc aaatgagaaa
      961 gtcgatttag taaaaatcat gattgcccac gaagcaaacc gcacgcagcg aaagatttct
     1021 ttgacagaag atgtagcaaa agcattgatc ggcagtgtga cctatggaaa tataggacaa
     1081 ttgaagtcaa atatccagtt agtatgtgca cgaggatttc taaatcatat gcaatcaccc
     1141 gaaatctcga tcacaatcga tgatttgaca gaagggattc gtagcggatt gattcaatta
     1201 gcaagcaacc gcagtgcaat ggcagagctt tctaaaatat tggagccaaa gataactgta
     1261 gcaccaaatg atacgatcat gaaaatccaa tcggactcct atgagttacc ttataatcta
     1321 tatgatatca tcggagataa agcagcttta ctgaagtcgg atggtttgga tcaagaagca
     1381 atcaatcatt tcatttctac agatatcaac atccatctga agtctttcta taaagaccat
     1441 ggtttttctt ttaatgcaga cagcaaatta gcagaatttg tagatccgaa aatcatagaa
     1501 gtgaccaatc agatttatgt aatggtcaaa agatctttac catatgagtt tcagcaaaac
     1561 tttatttatg ctatgagctt gcatatcagt tcttttttga aaaaaatcaa cttaggagaa
     1621 gaacggcata cgaatgataa catccgtgag atggcttttg attttccaga tgaatatgct
     1681 gttgcgaaag aagtgcgtag ttatatcgaa acgtattttc aagtggagat tccagaaagt
     1741 gaagactatt acttgaccgt tctactcgtc tcgttacggg caaatcaagc aagcggcaga
     1801 atcggtgtag tcgttgcagc tcacggaaac agcacagcaa gcagtatggt ccaagttgtc
     1861 caacagttgt tagatgcaga gaacgtacga gcagtagaca tgcctttgga tatggatccg
     1921 aaaacagcat tggaccgaat tgaacgcaat gtacaagaag tagatgaagg aagcggggtc
     1981 atactcttag tagatatggg gtcccttgcg tcgtttaatt cgcaaattca aagagacact
     2041 ggaattccag tacgtactgt tgatatggta acaacttctc tagttttgga aactgtacga
     2101 aaggtctcag tattagggac agatctagat atgttgcatg aatcattgaa aaattttcga
     2161 ggttacgcag aaatatcttc tgtagaatct gttcaggaag caaaacctat tcataagaaa
     2221 aaagctgttt tggctatctg cgcatctggc gaaggtacag cacaaagaat caaagaattg
     2281 atcgaacgag cggtaaacaa gcgtcaagaa acagaattag aagttctagc tttgtcgatt
     2341 gtggaagtga aagaagaact gccagttatc caaaaagaat atcagatcat agctactact
     2401 gggattactg atttgaatat ccaagcccca tttattcctt tagaacgttt tattgatcaa
     2461 aatatcgaag tcattttgga tcagttgctg atggaatcag aattggagga aactgaattc
     2521 atctccctag atgaagagtc agctaaaaat acgtgtgttg agtttatctc tgataacttc
     2581 atctttatta atggtagtaa gttaatcgat ccaatgtggc aattttctac acaaatctct
     2641 cagacgacgg gaatcggcga tgaagaatat ggattcaaaa tcaacctagt gatgcacaca
     2701 gcaggaatga tcgaacgtat tattcggaat gaaccattga ctgtggaaga aaacgaattg
     2761 acaaatacaa cgaatgatcc tctttatagc caattggcag cctcagttgt tctgttggaa
     2821 gatcagataa aagtaaaagt gccgatagaa gaaatgtatt atctgctacg tctagtccat
     2881 aatcagttag ataaaaaaga atacactgtg ccatgaatac acaaaaacga tacactttta
     2941 tagtgtatcg tttttgtgta ttggttaaga atcctttaaa ttcaattagt gtatcgtaaa
     3001 aaagttggca cgctttttgc ttatagtagt gtgtgctagg aggtggttgt tcacacagaa
     3061 atgatagggg tttctgtgaa gtagtgagga agaaggagtt ttgcagatgg atattcgatt
     3121 ggcacgtata gatgatcgtt taatccacgg gcaagtcgca acagtttggt cgaagcaaac
     3181 aggtgttgaa cgtatcattg tgatcagcga tgtagtcgct caagataaat tgagaaagtt
     3241 tctactaaaa gaagctgcac cgccggggat cacagcaaat gtaataaccg tgcagaaaat
     3301 gattcaaatc tatcatagtg gattattatc acgagcaaaa gtgatgcttt tattcaccaa
     3361 tccccaagat gttgaagcgg ttgtccgctg cggtgtacag ttgaagtcgt tgaatatcgg
     3421 cggtatgagt tttactgaag gcaaatcaat gattaccaat tttgtttcag taaatcaaca
     3481 agatattgat tcctttcgtt acttgaacag tcaagaagtg gagttagaga ttcgaaaagt
     3541 acctgctgac aggaaaatcc ttttgatgga actactagcg aaaatcaaaa aggtgtagca
     3601 agaatggttg ttggaaaagg tcaaataatt ctgttgtaat ggttagcaat tattaatatt
     3661 t
//
LOCUS       DACESX010000173         3590 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.177, whole genome shotgun sequence.
ACCESSION   DACESX010000173 DACESX010000000
VERSION     DACESX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.177"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            391..1941
                     /locus_tag="IXF88_002546"
     CDS             391..1941
                     /locus_tag="IXF88_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509242.1"
                     /translation="MVAVYEYLKKNIPGCDDNVNASNRMFFGGYKSDAVIIINEDNML
                     DITELPIDFTMTCSKNCQSDSGREYELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSD
                     MSFNEIYERLLTIDMNKLLKSSKNLRCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDI
                     SENFIGVVMLILKKDSRVETFNWLLENLDLYPTHFRKLKEESKVLFDTLESMKDKLFR
                     TIRNYLEEMRRVYDVLLSDVNFFDDSKESVTCILSGEQLAKKMSSYYGKTYDIDKCNK
                     LLSLMTFIGLIKKLDDEDVPLKMLEYLNRLKEEKALGNFNHKRSNVYRLDVFNATSVI
                     QKLNEDILPLLEKIHFTYQYFSYEWVKICFNEKEAKRVFPQNSRSELSKEKQLILDEA
                     HEVLQGLSWQGTYLMTEKELINTVCERLRNVGDNSVKNALIKNRGYFVKQGFTLERAS
                     KDIKKLYNVKASSSFLIYLAKGAGIDETIKELILSGAGIVPLYQFTSVTRDDGRRVRK
                     RKDPIVLN"
     gene            complement(2017..2352)
                     /locus_tag="IXF88_002547"
     CDS             complement(2017..2352)
                     /locus_tag="IXF88_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1509243.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            2479..2694
                     /locus_tag="IXF88_002548"
     CDS             2479..2694
                     /locus_tag="IXF88_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1509244.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            2731..3093
                     /locus_tag="IXF88_002549"
     CDS             2731..3093
                     /locus_tag="IXF88_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1509245.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            3139..3465
                     /locus_tag="IXF88_002550"
     CDS             3139..3465
                     /locus_tag="IXF88_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509246.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         1294 a          471 c          718 g         1107 t
ORIGIN      
        1 aagccagtta caaaaaaaga aaaaactgat gtaagagata ggtgtttaga taactataga
       61 gaagttacaa gagatgagtt tgtagagata ctaaattcat cacaatcatt tattccaagc
      121 aagacaaaga ataaaggcaa tgacaaggag agttttgtgg aaaccagagt aattattctg
      181 gatgtcgata acactgttaa agatgagaag gggaaggtag tagatttgag taaagatgat
      241 agtagatact taagcgtaga aaaggtttta tctatcaatt tagttcataa ttctgcgttt
      301 gcgatacaaa aaagtattcg gtactctgaa aatctagaaa aatacaagat tgtctttcta
      361 ttaaaagaag caattactga ctatggtaaa atggttgctg tttacgaata cttgaaaaag
      421 aacatccccg ggtgtgatga caatgtaaat gcttcgaata gaatgttttt tggaggatat
      481 aagtcagatg cagtgataat aattaatgaa gacaacatgt tagatattac tgagcttccg
      541 attgatttta ccatgacatg ttctaaaaat tgtcaaagtg attcagggag agagtatgaa
      601 ctagtcaatg aaaccgattt tgttaagttg ataaaaaatg acgacaaaga agaaatgaaa
      661 cagtggttta aagactgcct gtttgattcc tccgacatgt cttttaatga gatttatgaa
      721 agattgttaa ccattgatat gaataaatta cttaagtcaa gtaaaaactt aagatgtctg
      781 tttcacaacg atcataatcc ctctgctagt atttttacca gtaaaactgg gcattccatt
      841 ttttattgtc attccagtaa ttgtgatatc agtgaaaatt ttattggagt tgtgatgcta
      901 attttaaaga aagacagtcg ggtagaaaca tttaattggc tattagagaa cctagacttg
      961 tatcctacac attttcgaaa attgaaagag gaatcaaaag tactttttga cactcttgaa
     1021 tcaatgaaag acaaactttt caggacaata aggaattatc tagaggaaat gcgacgtgtc
     1081 tatgatgtct tgctttctga tgttaatttt tttgatgata gtaaggaaag tgtgacttgt
     1141 attttgtcgg gggaacaatt agctaaaaaa atgagttctt actatggtaa aacttatgac
     1201 atagataaat gtaataagtt actatcactt atgactttta taggattgat aaaaaaatta
     1261 gatgatgaag atgttccttt gaagatgctc gaatacctaa ataggttaaa ggaagagaaa
     1321 gcgttaggaa actttaatca taagagaagc aatgtctatc gattagacgt ttttaatgca
     1381 acttctgtga ttcaaaaatt gaatgaagat atccttccac ttctggaaaa aattcatttt
     1441 acatatcaat atttttctta tgaatgggtt aagatatgtt ttaacgaaaa agaggctaaa
     1501 agagtatttc ctcaaaattc ccgatcagaa ctcagcaagg aaaaacaatt gattttagat
     1561 gaagcacatg aagttttaca gggcttatcg tggcaaggga catatctaat gacagagaaa
     1621 gaacttatta acactgtttg tgaacgccta cgtaatgttg gggataacag cgtgaaaaat
     1681 gctttaataa aaaacagagg ctactttgtt aaacaaggat tcactcttga acgagcttct
     1741 aaggatatta agaaactata caatgtgaaa gcaagtagta gttttttaat atatttagcg
     1801 aaaggggcag ggatagatga aacaataaaa gaattgatat tatcaggagc tgggatagtt
     1861 cctctgtatc aatttacaag tgtaacaaga gatgatggac ggagagtaag gaagagaaaa
     1921 gacccaattg tattaaacta gacaggttat ccatagctct taatcttatt tcatctcaat
     1981 acacccaccg ttaaagttcg gtgggttatt tatttttcaa tctttcaata gctgattgcg
     2041 ttcattttct tgccttttta atagcttcat atagtcaatt gttcgaactg catcttcagt
     2101 gctaagagaa cgatagagag ccaaaaactc ttcctcataa tctacgacaa cattgtctag
     2161 aaaatggctt acaggggttc ctagggctct agaaagagct gttaagctat cgaggttagg
     2221 ctctgttaca gaattttccc atcttgctac tgcctgttgt gtagagaaga ttttttcagc
     2281 taattctttt tgtgtccacc cgagagcttt tcgcttttct ctaatgacct ttgcatattg
     2341 tttctgtttc attaatacac ccccatctca gtatagtaca acaagttgtt gtatttgtaa
     2401 tggtatttga agttttttat attttatgag ttgaaataca tcatattgtt gtatataata
     2461 aaacaggagg ggagaatcat gaaaaaagta agtaatctca gccgatacag aaaagaaaaa
     2521 ggactttctc aaacggagtt agcaaaaaaa atgaatgtta cacagcaatg tgtaagttcg
     2581 tggcaaacag ggagaacgat tcctaaaccc tatcaaatga agatgctttc tgagatatta
     2641 agtgtaccaa tgaatgagct gttttctgat gtatttaata aagtaaacag ttaactaata
     2701 agcaattgaa tgattttagg aggagaagac atgtattgta gaaaatgtgg caaagaaata
     2761 acagatgaaa gtaagtattg ccagttttgt ggagcactac aagaaacaga tgagacaacc
     2821 ggaacagaca cagtaaagaa aagtactgag cagacaatta atgaaatgaa ggaacaaatc
     2881 gtacatggac aagagacagg gaagctgtat ttgattattg gttggatttc tatggtagta
     2941 tcgttagcat ttattcctgt tgtatttggt gctgttgctg ttattatggg ctacttatat
     3001 cgagaaaaag atgagaaaat gggtacgatt ttgatgatag caggggtagc tggagcaatc
     3061 tttggtgtac ttttagggat gtcaatgtac tgataagagg gataagagca aattattttg
     3121 gagataggag ataaaaagtt gaataaagta aatatgaaca agaagtttgt cattataggt
     3181 attgttgtct tggctattat aggtattatt ttttcaaaaa tgacaggtac atctggatta
     3241 gagaagtcat caaaaccatt ggtaacacag attttacaag aacagtacaa cttaaatcga
     3301 tcatgtgatg atgtgacgat tactcatgaa aatggagata acacataccg ggctaaagct
     3361 atattagaca atggaagtgc tatcaacata aatattgagt actatccaaa gaaagaccgt
     3421 atatatgtgg agataccata tgctgaggtg ttaatgttaa attgatttaa atgtattatg
     3481 cataccgtcc aaacaggaac tcccaaaggt gtaaattcac tagggagttt ttatagtttc
     3541 ctataaatta aatactaata caggtgacaa aaaaatatta caatgattgt
//
LOCUS       DACESX010000174         3566 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.178, whole genome shotgun sequence.
ACCESSION   DACESX010000174 DACESX010000000
VERSION     DACESX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.178"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..2232)
                     /locus_tag="IXF88_002551"
     CDS             complement(<1..2232)
                     /locus_tag="IXF88_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509247.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="IXF88_002552"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="IXF88_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1018 a          782 c          552 g         1214 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaacaatca ttcttaaaaa gaatat
//
LOCUS       DACESX010000175         3495 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.179, whole genome shotgun sequence.
ACCESSION   DACESX010000175 DACESX010000000
VERSION     DACESX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.179"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(50..1516)
                     /locus_tag="IXF88_002553"
     CDS             complement(50..1516)
                     /locus_tag="IXF88_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1509248.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSSVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1903..2499
                     /locus_tag="IXF88_002554"
     CDS             1903..2499
                     /locus_tag="IXF88_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1509249.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2557..3039)
                     /locus_tag="IXF88_002555"
     CDS             complement(2557..3039)
                     /locus_tag="IXF88_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509250.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3145..3342)
                     /locus_tag="IXF88_002556"
     CDS             complement(3145..3342)
                     /locus_tag="IXF88_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509251.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT         1183 a          704 c          575 g         1033 t
ORIGIN      
        1 aaaacaggaa ctgctgcaaa taagcaattc ctgtttttat gttcattgat taatgaatag
       61 agcccattag ttttttgatt ggttctttca cagtaaatag caagatacca ccgacgattg
      121 ctactgcacc gataataccg aagtaagcag attctgttcc cggtgtgtag aagcgtgcaa
      181 tttgtgcatt gattgcttgt gaagaagcat cagctaagaa ccaaatagcg attgtttgtg
      241 attcaaatgc tcgtggtgct aattttgttg tcacagacaa accaactgat gaaagacaca
      301 attcagcaat aaccacgatg aagaaacttc ccaacaacca caatggactt acttttccac
      361 ttgtaccata aagcataccc ggcaacatca atagcaagta cgataatcct gcaaataaaa
      421 gtccaagaga gaatttcact actgtagatg gttgacgttc accaagtttc gtccacatcg
      481 ttacgaatac tggtgtcaga ataaagatga aaattggatt caatgattga taccagcttg
      541 cttggatcgt tacaccaaat agcgtagatt gtgtacgttc actagcaaat aaagctaaga
      601 tagatgaacc ttgttcttgt aatgaccaga aaatgattgc tgcaaggaat aatgggatat
      661 aagcccatac tcttggtttt tcttcagcag ttacatcttt tgaagtcagc attttgataa
      721 agtaatacaa cggtaagata atccccaata cactgattgt attgacaaaa aagtcaattg
      781 tcagatgacc agttagttgt gccccgccga atactgcagc gataacaaca agtgcgatga
      841 tcaagttacg gatgaatcct ttacgttctt cagttgtcaa aggattcggc ggtgttgtac
      901 ccactccgtc taatgtcttg cgtccttgga tgatgtattg taataatcca aagaacatcc
      961 caatagcagc taaagagaac cctaaatgat agttatattc ttgaccgatt gtaccaacga
     1021 ttagtggtgc taataatgcc cctaagttga cacccatgta aaaaatcgaa aatcctgcat
     1081 ctcttcgcaa gtcagtcttt gaataaagat gtccgaccat gccagaaaca tttggtttca
     1141 aaaaggcggt tccgataata atcaatgcca tcgaaacaaa caatgtactt acaccaaatg
     1201 gtaaagccaa gacgatgtgg ccaagcataa tgaaaactcc accgaagaat acagtgcgtt
     1261 ttgctcctaa cactcggtca gatacccatc cgccgacgat actagacatg tagatcaaag
     1321 atccgtagat cgacatgata gctaatgctg ttgctcgtgg taatccaagt cccccgttgg
     1381 caactgtatc gtacatataa tagataagta atgcacgcat accgtaataa ctaaatcgtt
     1441 cccacatctc agtgaagaac aaagttgaca atcccttcgg ctgcccgaag aagcccttgt
     1501 cttgctgttt ttccataatc tataccccaa ttctttaaat tttcagaaaa tattatacat
     1561 ctttaccgaa aaatttaact aaaaaactta tttaagaaat ataaactaat tttctataaa
     1621 agcaagtaat ttttaatggc aatatcataa aaaaaagaag atagagattt aataattata
     1681 tatcagctgt ttttttcaaa taagcaaaaa ttcctaaaac acaacaatga aagcgataac
     1741 taagaaaatc cttacataac gatttttctt attaaaattg ttttttgaac ttcgcttatt
     1801 ttttgtgagc atatctgttt acttactttt tgtaaggtgc tatacttcat ttcgtaatca
     1861 attaaaaaaa tgcaaaacgc aaatcaaagg agaaatttta ttatgacaac ttttactgac
     1921 actttgaaaa accgccgttc aatctatcat ctaggacgta atgtttcact ttctaatgaa
     1981 gaattaacaa ctttgatcaa agaagcaatt aaagaaagcc caactgcttt caatgctcaa
     2041 tcaacacgcg cagttatcct tttcggagat gcacatgaaa aactttggga aatcactgaa
     2101 gaagcacttc gcccattgac tccagcagaa gctttcccaa atacacaaaa caaattagct
     2161 ggtttcaaaa atggttatgg tacagtatta ttcttcaaag atactgacgt ggtaaaaggt
     2221 ctacaagaac aatttgaatt atatgcagac aacttcccag attggtcaga acaatcaaac
     2281 ggaatcgcaa cagctaatac ttgggtagca ttagttgata aaggattggg cgcaaacttg
     2341 caacattaca acccagttat tgacgaagca gttgcaaaag aatggaatat cccttcaaac
     2401 tggaaactac gttcgcaatt agtattcggt tcaccagaaa caccggctgg ggaaaaagaa
     2461 tacatgaacg atgccgatcg cttccgcgta tttggataat tttatttgaa ataaaaaaca
     2521 gaggttgtga caaacatctt gtcgcaacct ctgtttttat tctggatcaa tcataatatc
     2581 aatctctgcc tcatcgttta agcgttggga caaaaatgga actaattctt ctaatgatgc
     2641 agaaaatttc agcatcggtc ctcgccatcc gtttcggaaa aaattcattt ttacgatttt
     2701 agaacctttt ggctgatata atacaatttt attgatctca gaaaaaggaa cacctttttt
     2761 gtcaaagcta ttcaaaacaa gtccttcttc tgtgattcct ctactgtcca acaaaaaact
     2821 aataaaaatc attgctgaca agatcccttt tgccaattca tctgctgtag gatgactgat
     2881 gagtgtatac caaatgacta gtgacactaa gaagggtatc accaagtaga cccatcgatt
     2941 tcgcacagtc cgataaataa tttcttttcg aaccagccaa agaaataaaa acatcacaac
     3001 aacgaataaa aacaatagaa ccgtaaagaa tgttaccatg acaactcgcc tcgctttttc
     3061 ttctccagaa aataggaaaa gaatactgat ttaccttctc aaagaaattg gacttgcagc
     3121 acgccgcacg tccaattgtt ttctttattc aaatgtatca tattttgcaa caaccatgct
     3181 tgctttttca tctaataatt cttggtcctc tgcagcacat tgggcaactt tgaccacggc
     3241 gcagtttgtc atttttgata caacttcacc aatgaccgtt tttgtaaaac caattggttt
     3301 gcatgcatat tgttctccta aattaatcat ttcttccgtc atcaaaatcc tcctaaactg
     3361 ctggaacttt tctccacatt accactgcta tttctttgga gatttaactc attgattttt
     3421 atttacagtt aaaaatcaac cttacaggca ttatagcgta ctgttttcgt tttcacaatt
     3481 aaaacagctg ttgta
//
LOCUS       DACESX010000176         3462 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.180, whole genome shotgun sequence.
ACCESSION   DACESX010000176 DACESX010000000
VERSION     DACESX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.180"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..182)
                     /locus_tag="IXF88_002557"
     CDS             complement(<1..182)
                     /locus_tag="IXF88_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1509252.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="IXF88_002558"
     CDS             517..951
                     /locus_tag="IXF88_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1509253.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="IXF88_002559"
     CDS             974..1294
                     /locus_tag="IXF88_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509254.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="IXF88_002560"
     CDS             1435..1626
                     /locus_tag="IXF88_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509255.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="IXF88_002561"
     CDS             complement(1697..1954)
                     /locus_tag="IXF88_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1509256.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="IXF88_002562"
     CDS             2112..2375
                     /locus_tag="IXF88_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509257.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="IXF88_002563"
     CDS             2399..2587
                     /locus_tag="IXF88_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509258.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="IXF88_002564"
     CDS             complement(2670..2855)
                     /locus_tag="IXF88_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509259.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="IXF88_002565"
     CDS             3070..3441
                     /locus_tag="IXF88_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1509260.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT         1294 a          492 c          698 g          978 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcgtcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga at
//
LOCUS       DACESX010000177         3459 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.181, whole genome shotgun sequence.
ACCESSION   DACESX010000177 DACESX010000000
VERSION     DACESX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.181"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            2..454
                     /locus_tag="IXF88_002566"
     CDS             2..454
                     /locus_tag="IXF88_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509261.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            468..1157
                     /locus_tag="IXF88_002567"
     CDS             468..1157
                     /locus_tag="IXF88_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509262.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1282..>3459
                     /locus_tag="IXF88_002568"
     CDS             1282..>3459
                     /locus_tag="IXF88_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1509263.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDE"
BASE COUNT         1169 a          461 c          729 g         1100 t
ORIGIN      
        1 cgtgaaaaaa acgatactaa gtattttttc agtatttaca atattaattg ggggctattt
       61 tcttttgaat ttagattctg ttaattcaaa cgctcaaata aaagatcaaa gagtagctga
      121 acataataat aaaaaaatga gtttgaccaa aaatatctat tctatgtcag atgaagaatt
      181 gtataaagaa tttggtgatt tatccgggga acagttagaa gaaagtattc caggaattag
      241 tgactcagat aaaattgttg tcttgtctgc tggtgggcaa ttaatgggag atggcaaatc
      301 cgaattagtt agttataaac aaaatcacag acagtttatt gatgttaata ctgattccaa
      361 cgctatttcg attaaggatc taaaagagct tttaaatagt aaaagaaatg aattaaagca
      421 gataaaagaa actaattcag tggaattaga gtaaaggaag attcattatg aaaaagattt
      481 ttggtgtttc tgttgggcta cttatgatgt taacatttgg aaatgcagta tatgctgctg
      541 agaaagaagt tatttcagat ccagagcagt acgcctcacg atactttgct gattttgaag
      601 aagaagataa agtacttgca ttgccagaag gtggtttttt aaaaggggaa gctaaaattg
      661 tatctatgga taatccagat ataattcaag gttcatataa ttcagaaact gatcctgctt
      721 cagtttcagt gacagaagca aaaaaaattg ttgctgaaaa attcaaaaat gatattgaaa
      781 ctactccaat tatgcctcga ggagcatttc cacccgatgt tggtactagc ggacaccttc
      841 ttgtactagc aggaggagga agctataaat cacagccatt tagtggtgcg ggatggcgtt
      901 ttggaggtat gggtgtaaaa ccagctgatg gtacaggcaa ctttttacgt tggaaatcct
      961 acattgacga tggaagagta ggaaatgtaa atcaagccaa cgcaactaaa tctggttcta
     1021 ttcaaggaat ggcattgtat gctcgtgaag gattcaaata ctttgcacca aacgggcctg
     1081 gttggacaat gaatataggt atttattaca cttataatcc tgttgcaggt acgtattatg
     1141 ttgttcaaaa tatttaataa aacagcacaa agagatttct ttgtgctgtt ttattatttt
     1201 tagttgttga caaacactaa aatataaaat ataatcaaca cataaacgaa cgtatgttcg
     1261 gcaagaaagc aggggatttt tatggatgag atggttttaa gtactcagaa atggctaaac
     1321 aagaaataca gtaatgttac agggtttgat aaggttccag aaaatgggcg aacagggtgg
     1381 cctacaatct atggacttat tgaaggacta caagtagaac ttggtattac taatctagta
     1441 gctaattttg gacctaccac tgaaaaaatg tacgataatc aggttactcc taaatggggc
     1501 aaaaatctac ctaaaaatat tgtatttctt attcaaggtg ccttttggtg taaggggatt
     1561 aatccaggtg gcttcgatgg ggtatatact ccatatttag atactgctgt aaaagagttg
     1621 cagactgatg cagggtttaa tggaagcgct gtaactggag ttttggatgc gaaatgggcc
     1681 aaagcgttat ttgatatgag tgcttttgtt cttgttcccg gcggagatag taaaattaga
     1741 aaaatgcagc aatggttaaa tgtaaattat ttagaataca cgggaataat gccttgtgat
     1801 ggtatttatc aaagaaattc aaatcaagca ttaatttttg cattacaagc tgaactagga
     1861 tattctccca gtgaagcaac agggtcttat ggtaatggga caactagtaa aacttatcct
     1921 gtgtctgaag gtaactcggg aaactatgta agaataattc aatatggatt atatgttaat
     1981 ggttattttg aagacggtac attcgatggt attttcacta aatatatggg tttagaagta
     2041 ctggcattcc gaaaatttat ggtattgcct gaatatactg aaatagcaga tgttgttgta
     2101 ataaaaggac tattgtctag cgctggagat attagaagat ctgcagatgg cgcagatact
     2161 tctacgcaat taactagatc tcaaatccaa acattggttg ataatgatat aaaaatagtt
     2221 ggtagatatc ttactggtac tgttggaatt ggaaaagatg aacgaaataa gtatctaact
     2281 acagaggaat tgaataatat tttttctaaa aatttatctg tgtttcctat ctatcaagat
     2341 ggaggtgcag cattagctta ttttacttat gatcgtggct tatcagatgg taagaaggct
     2401 atagatgcag caaaaaatct aaatatacca ttaacaacag ttatttattt tgctgttgat
     2461 ctagacatgt taggggaaga aattcttgca tatgctggag aatattttaa gggggtatct
     2521 gctgtaatgt catatagcgg gtatcaaacg ggtgtttacg gaacaagaaa tgtttgttct
     2581 caaataatca ataacgggta tgcttcgttt gcatttgttt cagatatgtc gactggttat
     2641 agtgggaatt taggttttcc tatgcctaga caatggtctt ttgatcaatt tgttgaattt
     2701 accataggaa gtggcaacgg agctgttggt atcgatttaa tcgctacatc gggtagagat
     2761 agcggattta atgagttatc aaatgatact aataatgata attacattgc caaatacaat
     2821 cagaaggtta taaatcagat ggtacgtgcg tatcaatatc tatcacaagc tggtcttgat
     2881 aatccttgga accctcattt gacattttat agatatgtaa attattctgg tttgtcatgg
     2941 gacatcattt cttctcctgt aacagagcat gatagaaaaa tttatgatga atataggaat
     3001 aaattaacta atagcgaggg cctgtataat tattttatag atcctaatac tggttcaata
     3061 ataggtttgc ctcacttaat tgtgacgtta caatcacaga tgtttctagc cgacacaatc
     3121 aatgatagtg tttctgactt tgcaggttgg ttaggagatt taatgacttg ctggggtgaa
     3181 gttaagaaat taggaataca aatggagcaa ggtgtatttg cattagtagg tacggctgca
     3241 ggtggaacat ttagtctaga agatttatac caagatgttg acgcggtaaa tgtttatacc
     3301 ttagcaaaaa gaaattctga ttcaacagct ttatcggctt taaaaacgta ctattgtgat
     3361 aagggtgcta ttgatagatt taatcaattt gttaagaaca gatttggttc ttcagataaa
     3421 atcgcttcag taacaaatag tttattagaa aatgacgaa
//
LOCUS       DACESX010000178         3369 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.182, whole genome shotgun sequence.
ACCESSION   DACESX010000178 DACESX010000000
VERSION     DACESX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.182"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            167..949
                     /gene="rpsB"
                     /locus_tag="IXF88_002569"
     CDS             167..949
                     /gene="rpsB"
                     /locus_tag="IXF88_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1509264.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1048..1929
                     /locus_tag="IXF88_002570"
     CDS             1048..1929
                     /locus_tag="IXF88_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1509265.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2065..2787
                     /locus_tag="IXF88_002571"
     CDS             2065..2787
                     /locus_tag="IXF88_002571"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1509266.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            2790..3347
                     /gene="frr"
                     /locus_tag="IXF88_002572"
     CDS             2790..3347
                     /gene="frr"
                     /locus_tag="IXF88_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1509267.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1239 a          585 c          757 g          788 t
ORIGIN      
        1 tatttgctct tgcgatacgg atcttttgtc tgtataatag ctgagggtgc tttatgcatc
       61 agcaaatcca cattcttatt ggattgtcgg taggtgcttg aaaaagtttg ccggcaaggt
      121 agaagataag aagaggaaaa ataaaaccaa atcggaggaa acaaacatgg cagtaatttc
      181 tatgaaacaa ttactagaag ccggcgtaca ctttggtcac caaactcgtc gctggaatcc
      241 aaaaatgaag aaatatatct tcacagaaag aaacggaatc tacatcattg acttgcaaaa
      301 aacagttaaa ttagtagatg cagcttatga ttacatgaag agcgttgctg aagaaggcgg
      361 cgtagcatta ttcgtaggta caaaaaaaca agcacaagaa gcaatcaaag aagaagcaac
      421 tcgtgcagga caatacttcg ttaaccatcg ttggttaggc ggaacattga caaactggga
      481 tactatccaa aaacgtatca gccgtttgaa acaaatcaat gcaatggaag aagatggaac
      541 ttttgaagtt ctacctaaaa aagaagttgc tggtttgaac aaagaacgtg aacgtcttga
      601 aaaattctta ggcggtatcg ctgatatgcc aagaatccca gatgtaatgt acatcgttga
      661 cccacgtaaa gaacgtattg ctgttcaaga agcacacaaa ttaaatatcc caatcgtagc
      721 tatggttgat acaaactgcg atccagatga aatcgacgta gtaatccctt caaacgacga
      781 tgcaattcgt gccgttaaat tgattacttc aaaaatggct gatgctttca tcgaaggaaa
      841 ccaaggtgaa gatcaagtag ttgaagaaga tttcacagaa gaaaacacag caacatcaat
      901 cgaagaaatc gtagatgttg ttgaaggcga caattcttca gcagaataat tttgaatgat
      961 ggagctgttt caaaggctgg gcgacaagcc tcactctcct ttgaagcagc tttttttaaa
     1021 aagaaaaaca cacaggagga gttaaacatg gcagacgtta cagctaaaat ggtaaaagaa
     1081 ctacgcgaaa tgactggcgt aggaatgatg gatgcgaaaa aagcattagt tgaagtagaa
     1141 ggcgacatgg aaaaagcagt tgatctttta cgtgaaaaag gaatggcaaa ggctgcgaag
     1201 aaaaatgacc gcattgcagc tgaaggattg gcttcagttg ctgtgaaagg taacactgca
     1261 gcaatcgttg aagtaaactc agaaacagac tttgtttcta aaaacgaaat gttccaagat
     1321 ttagtaaaag aaattgcaga acttgttgct gaaaacaaac cagcagacat ggaagctgca
     1381 atgaaaatca aaacagataa aggtacaatt gaatctgact tgatcgaagc aactcaagtt
     1441 atcggagaaa aaatcagctt ccgtcgcttt gaagttgtag aaaaagacga caatgctgca
     1501 tttggtggat acttgcatat gggcggacgt attgccgtat tgactgttct agatggaaca
     1561 actgacgaaa cagttgcaag agacgtagct atgcacgtag cagctatcaa tcctcgttat
     1621 gtaaacgaat cacaaattcc agaagctgaa ttagaacatg aaaaaactgt tcttacagaa
     1681 caagcattga acgaaggcaa accagcgaat atcgttgaaa aaatggtaga aggacgcttg
     1741 aagaaattca aagcagaaat cgctttggtg gaccaaccat tcgttaaaga tcctgatatg
     1801 actgttgaaa aatacgttgc atcaaaaggc gcaactgtta aaacattcgt acgtttcgaa
     1861 gttggcgaag gtatcgaaaa acgcgaagat aactttgtag aagaagttat gagccaagtg
     1921 aaaaaataat tctgttgaat aacagacggg aacgcacggt ttgtgcgctc ccttttttta
     1981 agcaaaacaa gaaaaaccag tcttagagtg tcgaaatcat ctaagtatgg taaaatggca
     2041 atagaaaata ttggaggaat gaacatggtc acaccgaaat atcagcgtgt agtcttaaaa
     2101 ttgagtggag aagctttagc tggggatgca ggatttggaa tcaaaccacc agtaatcaaa
     2161 gaaatcatcc aagaaatcaa agaagttcac gagctgggcg ttgaaatggc aatcgttgtg
     2221 ggtggcggaa atatctggcg cggacaaatc ggagcacaaa tgggcatgga acgtgctcaa
     2281 gctgattaca tgggtatgtt agctacagtt atgaatgcct tagctttaca ggatacgttg
     2341 gaaaacgtgg gtgtaccgac acgcgtgcaa acttcgattg aaatgcgtca aatcgcagag
     2401 ccatatattc gccgtaaagc agaacgtcat ttagaaaaag gacggatcgt tatctttgca
     2461 ggcggtaccg gcaacccgta cttctcgaca gacacaacag cagcattacg agcggcagaa
     2521 atcggcgcag atgttatcct gatggctaaa aacaatgtag acggtgttta ttctgctgat
     2581 ccgaaagtag atgcaaatgc agtaaaattt gaagaactga ctcatctgga agttattgcc
     2641 aaaggattac aagtcatgga ttcaactgca agttcactaa gcatggataa tgatattcca
     2701 ctgcttgtct tcaatttaaa tgaacacgga aatattcgcc gtgcaatctt aggcgaaaat
     2761 atcggaacaa ctgtaagggg gaaataaaaa tggcagatgc aattatgaca gaagcaaaag
     2821 aaaaaatgaa aaaagcagca caaaatcttc aacgtgaact tgggcaaatc cgcgcaggcc
     2881 gtgcgaacgc tagccttttg gaccgtatta ctgtgaacta ctacggtgcg ccaacaccat
     2941 tgaaccaaat ggcatcaatc caaattccag aagcacgtgt tttaatgatc acaccttttg
     3001 acaagagcat tcttcaagat gttgaaaaag ctattatggc aagtgatatc ggaatcagtc
     3061 caaccaatga tggaaatgtc attcgcttag ttatcccgca actgacagaa gaacgcagaa
     3121 aagaattagc taaagatgtg aaaaaagaag cagaaaatgc aaaaatcgcg attcgtaaca
     3181 tccgtcgtga tgcgatcgat gaatataaaa aacaacaaaa aaatggtgat attaccgaag
     3241 atgatttgcg cggattagaa aaagacgtac aaacattgac agataacagc attaaagaag
     3301 tagataagat cgctgctgat aaagaaaaag aattattgga agtttaataa aatcataaaa
     3361 acagagggg
//
LOCUS       DACESX010000179         3323 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.183, whole genome shotgun sequence.
ACCESSION   DACESX010000179 DACESX010000000
VERSION     DACESX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.183"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(8..871)
                     /locus_tag="IXF88_002573"
     CDS             complement(8..871)
                     /locus_tag="IXF88_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1509268.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1067..3166)
                     /locus_tag="IXF88_002574"
     CDS             complement(1067..3166)
                     /locus_tag="IXF88_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1509269.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT          730 a          667 c          571 g         1355 t
ORIGIN      
        1 gggattctca attagatttt ttgactagtt ttgtcacacc gttttcttgt gtaatcaatc
       61 cttgtttcaa aagattacct aatgcgcgct taaatgctcc tttgctgata ccaaaagctt
      121 tcatgatttc ttctggatcc gatttatctg taaagttgat ttgctgatct gcagaacgtt
      181 ctaaaatggt taaaatcatt tgtgcatcat ctgatatagc ttcatatgct cttggcttca
      241 atgaaagatt cagtacaccg tctggtcgga caccaatcac tcgacctttt atctgctcgc
      301 ctagacgagg ttcattatat cgttcagaag gatggataaa cccaagatag aaatcttccg
      361 ttaaaacgta cgtacctgtt aatttcaagc ggtatgctgt tcctatcaca tctttgtttt
      421 tcatttcttc tgttccatat ttgcttaaag atttgaatat tttttcatct gctaaactgc
      481 cccaaatacg gtcttttgca tcaactctaa gggaaatcat caagcgatcc ccttttttag
      541 gccatagctc tgtcattgtc ggcaattcat caagagatac gacaaagtct ttatctggaa
      601 gaccgatatc gacaaacgca cctaaatctt ttcgtgtagc agtaacgatt ccaaaagcgt
      661 aatgaccgat acggctttta ggaatttgtg tagtgatccg attttcatgt ttttgatttt
      721 ggtaaccgaa tccttcaact gcttcaccga ttttgtgttc accttctgat ttgcttaagc
      781 caaaggtttg tccgtttttt tggacaaagt agtttttttc attttcatca ataataatac
      841 ctgtaaatat agttgctaat aaatcgttca tttttttctc cctctatagt gaaaggtttt
      901 cttaatacat ttctttgcta tcataacata atgttatcta cttgaatagc tgagccttgg
      961 tttggaaagg tgaaaaaagg agtatgacaa cagttgtgtc atactcccct ttttcgaata
     1021 aacggtgtta aacggcaggc tcacagcctc tttcatttgc ctaaaattaa ttgctcccat
     1081 ttcctccgaa ccatccagtt ttacagttga tggttacgat gtaaaatggc aaagcacttt
     1141 ctccaatgcc tttcatcaat gcagattttg ttgaagtggc atatagatta taatagcctt
     1201 ccccattttc gattctggct ttttctaatc tgtaacctcc ttcaacgata tatccacgtt
     1261 ggatacatgt agcaatcact ttgctttgga cacctggtgc ccacgtccat gctggattag
     1321 atgcatcggc aatatcactt gctctggcag atttgattgg ctgattcgct gagtttgaag
     1381 aggaaggggt cgaggatgcc gcttcttgct gtgctgcttg tgcgaccgca gcttgttgag
     1441 cggcttgagc tgcagctgct gcttgagcgg cttgttttgc ttgttcttca gctgcttttt
     1501 tcgcttcttc tgcttttttc ttttcctctt cttctttttt ctctttatca gtgagcatct
     1561 tatctacttg tttcaattgt tcattcaact gagcagatag atctgtattt ttcacttttt
     1621 ctactgcttc ttttgccgtc ttgtattggt cgcgtgttgc tgaatccact acttttccgt
     1681 ctttaaagac tatagctgtt ttttctttag ctgtttgcaa ttcagcatat tgatttttag
     1741 cttctgtttg tgcttgttga atcaattttc caaaatctgt gttatccgtt tcgattgctt
     1801 ggatctcttt atctgctttc aacttgatgt catctgctag atgatcatcg acgatggcag
     1861 gtgctatgaa ccactcattc actttttgga tatctcggat cttagattgg atatcttggt
     1921 aagttgtttc taactctgaa tatccttttt cattcttaag actggataaa tcatttttta
     1981 gttttgataa atcttgatga atcatatctg cacgaataaa ttgatggtga tcatctgtat
     2041 aaaaggcagc aagtttctca tgaagttctt ccatttgttg ctgtcttttt gcttctgctg
     2101 ctacttcggc ttctactttt tgatgatggt cataataagc ccagcttcct ccaccgagta
     2161 gaagtactgc tgctaaaacg agataaggta ctttttttga ttttttcttt tgcgctttat
     2221 ctggatctgt tttttctaag tttgattctt gatgtgtttt ttcagtatct ggcacgattg
     2281 ttattgattt cgcttctgct tcaaacagct taggctggtt ctcttctttt tctatttttg
     2341 cttcaagatc agtaccctct ttgcttacag attgattttc agcatcggat tcactattta
     2401 tttcatcggg ctctgttgac tttttgtctg tgttttcgag tatttctttt ttctctgctt
     2461 ccggcagttt attcgttttt tccttttcta attgtttttc agtaaatact tcagtttttt
     2521 ctgtgatatc cgtttttacg tcttttattt ttactgtttc tttacctgtg gcgttttctc
     2581 tatcagagtc tttttcagtt tcttgttcct tttctggctc tactttgttt tcctgtgcaa
     2641 ttttttcact gatcattttg agcagctctt tcgcgtgctc ttcttttgta tcttcacgat
     2701 gtgctttgat ataagcggat agtatcgaat tttcttcctc attatcgacg atttctttca
     2761 gttcagctac tcttcttttt tgttttgatt caagcgtttc ttcttgattc tttcctgatt
     2821 cttccgaagt ctcatgaaca gttgctttct tctgttcatt tttctctata ttagttactt
     2881 gatcgtgagc aggtggttct tcgtcttctg gttctgcttt ttcgaataat tccattacag
     2941 attctagagg taaatctttc aattccgacc attcgattgg atcgtttaat tctgtatcag
     3001 gaaagactgc atcttcagtt gatttatcct gcatagctgc atctgtattt gtttcttcaa
     3061 ttgattcttt tatttgctga tcttctgcag ttatttgatg accgcagtct ggacagatag
     3121 tattctgctc catttttttc gatccgcaat ttggacattt tttcatcact tagcgatcgg
     3181 ctcctttact aggtttacgc taccatttaa tctatcatat tctatacgtc acgccaatag
     3241 cttattttta ggatttactt aaaaataatg aatgaatttc cttcctatag aaaaaaaagc
     3301 ttgagactgc gtagtctcaa gct
//
LOCUS       DACESX010000180         3210 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.184, whole genome shotgun sequence.
ACCESSION   DACESX010000180 DACESX010000000
VERSION     DACESX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.184"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(262..630)
                     /locus_tag="IXF88_002575"
     CDS             complement(262..630)
                     /locus_tag="IXF88_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1509270.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            780..1262
                     /locus_tag="IXF88_002576"
     CDS             780..1262
                     /locus_tag="IXF88_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1509271.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1299..2309
                     /locus_tag="IXF88_002577"
     CDS             1299..2309
                     /locus_tag="IXF88_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1509272.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2424..3137)
                     /locus_tag="IXF88_002578"
     CDS             complement(2424..3137)
                     /locus_tag="IXF88_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509273.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1083 a          519 c          622 g          986 t
ORIGIN      
        1 atcactgata ttttctcgta ttttttcctt attcaaaagt tttgcaggaa aattgctgaa
       61 aaaataattt tactaagtgg aaaattcaaa gccattcaat cagtgatttt tttcagcaca
      121 agaaagactc cttataaaaa tagcagatac tttgtcaatt aagtgtttaa ccagggatga
      181 taaaaaggat aggattgaac aaaacctgta taagctgatg aatagtttcc tcgccagtaa
      241 aaggtttatt cgtataaagc tttatcgcca aaatttatca tgaccttttt tatcttcaat
      301 attaaatgaa atcaattgtt caatcaattc ccagttgatt acacttttcc aaggaacttt
      361 gatgacgtta tccgtatata cgtagcctgc ttcttggata tttttttcaa gggcacgaat
      421 cgctgctttt tccggtgcga tagaaaaatg atttttagca aaactaaatg caataataaa
      481 ggtaccatgg tcagtaaaca taggttggtt ccattttaca gttgtgtcta gctggggata
      541 cgtttctgtt atcttagcaa aaaggtcttt gatcttttgt tgatgttctt tttctttgat
      601 ctgattgatg atcggctgaa atttttccat tctaaaacgc ctccattaag tggtttgatt
      661 tattttacca gaaaaacaat agttatgtat aaaaaacgaa caaaacacaa gtaatgttgt
      721 aaccgctata tttatatgat aagatgtctc aaataataaa atagaaaaga gacataaaaa
      781 tgattgactt ggcaaagaaa ttcttacacg aaaatatata ttttgagttg tatcatcatc
      841 gagccattta tacaaatgaa gatgctttgg taataaagaa agaacaagga tttacaggta
      901 cagagaccaa aagcttattc ttaaaagata aaaaagataa taactacgta tatctgactt
      961 ttactacgaa acaaacggat ttcaaagcat tagggaagat cgtcggtaaa aggctttcta
     1021 ttgtttcatc agaacgcatg gaagaacaaa cgggacaaaa gcagggcgct gtttctccgt
     1081 ttggttatga aaaacagatt ccgattatta ttgatgaaga gctcttatca aatgaaaaac
     1141 ttgtttttgc accaggtaga ccagatcaaa cgatggtcat ccaaacggtt gacttaaaca
     1201 aaatcatcca gctacttgag atgccgatat ttttgatgcc ggcaaacgaa aaaaacaatt
     1261 aaccaagtaa aatagtgcat acttggaggt gaagcaacat gataagttat ccattattag
     1321 aaagaaatgc aacgattggg gttactgcac catcctcagg cgtatcagag gaacaccatt
     1381 cgttactcga atcagcttta aagagaatgg aagaaaaatc tttccaagta gttactggaa
     1441 agacgctttg gacgcaagat aaggaaaaat caggtccagc aagaaaacga gcagatgaat
     1501 tcaatcaaat gatgcaagat gaaaatatcc aaatgatctt tccgccttgg ggaggagaat
     1561 tattgattga gattctcgat tttgtcgatt ttgatgctct acaaccaaaa tggatagtag
     1621 gttattcaga tattagtacg ttgctacttg ctgtcacatt aaaaacaggg atagccactg
     1681 cacatggaac caaccttatt gatttaagag gagagtactt ggatgaaacg acagcaatgt
     1741 gggaaagtgt actgaaaaca agagaaggag aatcgattct acagcaacct tctgaaaagt
     1801 atcaaaaaga gtggccccat gagcattcat catcttctgt tttccatctc actgaaccaa
     1861 caaattggca atcagtcagc ggagatgcag taaggatgaa gggacgatta ttaggtggct
     1921 gtattgatac gatcagtcat ttaatcggta ccccttatgg agatgtccaa tcttttaaaa
     1981 gaaagtataa tgatggaaag ccgatcttgt ggtttctaga aaattgtgag ctatcgtcag
     2041 ttgatttaag aagatcactt gttcagatga aactggcagg atggtttgat ggcagctcgg
     2101 ggatcatgtt cggtagaagt gcagcagagg atttgttaga ttataaaatg aaggatgtat
     2161 atgaagatct gtcaaaggaa ttaaaactac cgatcttgta tgatattgat tgtggtcatg
     2221 taccgccaca actgacattg atcaatggtg cctatgcaga agtagcggta gaagatagaa
     2281 aagcgactgt tttgcaaacg tttatttaaa aaattttttc ctgccttgga gtttatcaaa
     2341 gaccaacaaa tctaaataga aaagaaaatg aaagaagctg acagtatttt gccagcttct
     2401 ttttgtatga tctattagaa attttaatta tccataacta tcacattgat cagctgtttc
     2461 acatcatttg gattacctgt aggaatagct actacagatg cattatcaat aatttcttga
     2521 gttgtttctc taatgaacgc tggataagat gtttgatctg caggcatttg ccaatgtgaa
     2581 ggaactggat aactagtgat tgtcccgtca tggttacttg aatacactaa aactccctgg
     2641 taactgtatt tcccagtgag cataaccaca tctttcggat agactgcact ccctgcatca
     2701 taaggggcga taggtgttcc tgcaggataa tgaataacat gtagttcgaa atcacccgat
     2761 gtatctctaa aagtattacc tctaattgct agccaaacac gtgcgtactc aatttgtaga
     2821 tcagaatatc ctgctaacgg atctattgac tgagaagact gtgaggaagt tgtgtctatt
     2881 gttgaagaag aaatatttgt ctcaactgta ctactagatg tagaagaagt attgggctct
     2941 gagactttac tgcttgttga agatgtattc gtttcagtag aagctgattt ctgagtactt
     3001 gatttttcag ctgaactggt ttgtttgatt gaacttttgt ctgtggtcgc agatggatca
     3061 ctattttctt tactaggaga cgtacatcct acaaggaata aagagaccaa caataaaata
     3121 aaacctaact ttttcatata aaatacctca ttattttttt aataccagta tagcataatc
     3181 ctgaattttt gaatccgtaa aaccttgtct
//
LOCUS       DACESX010000181         3200 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.185, whole genome shotgun sequence.
ACCESSION   DACESX010000181 DACESX010000000
VERSION     DACESX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.185"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(98..328)
                     /locus_tag="IXF88_002579"
     CDS             complement(98..328)
                     /locus_tag="IXF88_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509274.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(422..760)
                     /locus_tag="IXF88_002580"
     CDS             complement(422..760)
                     /locus_tag="IXF88_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509275.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1688..1804)
                     /locus_tag="IXF88_002581"
     CDS             complement(1688..1804)
                     /locus_tag="IXF88_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1509276.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(2280..3158)
                     /locus_tag="IXF88_002582"
     CDS             complement(2280..3158)
                     /locus_tag="IXF88_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509277.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         1010 a          588 c          469 g         1133 t
ORIGIN      
        1 ctctttatac tgcattaaag ttagtaatat taacagcaaa gatatcgaaa aaacaataac
       61 ataatatgtg tcaaaatttt tcataataag aactccctta ctgttgcata ctaacataat
      121 ccaaaaactc ttctacttca gctaaagaca ttccactttc tttagtccaa aaaacagttt
      181 tttctatcga tccttctctg taaactgtaa atgtcggagt cgagttaatt gagtagcgac
      241 tcttgaattc tttccactca ttttcatctt ctctaacttt tgctacattc acgtaataaa
      301 tattttcttt tcccttttct accagcatat cgaatagctt cctgcttttg atgatcgatt
      361 tcttgtgcta catctagtaa attttcatca atttctatat cataaagtat ttcactagtg
      421 tctacatcca caatatatac tttttatcag tagcaatttt tgagtaatcc gttaaaatga
      481 tctcagtctc gtcactttca tcagtataaa tatacgttaa caatgaactc accttttcaa
      541 tcggtgttag ctctaagcct actgtatttt ttactattga gtcattattt accaccataa
      601 cttcgtactg tatataccat cctatttggt ttccatcaat atcagtaatt ggctcactag
      661 ctaatggagt ctccggaaca atattttcct gatatttctt aaactcttct gtagcacgaa
      721 aaaggttatc atgttcctca cttgaaactg gttgagtcat agaaaatgtt acttggctaa
      781 acagagggaa aataaacact acaatcaaca aagtataaaa aaaagccctt ctttttcatt
      841 ttttatcttc caatcacaaa ttgaaaaact tattcctgca actttgagtc tagtatcaca
      901 atcgactgac tacaactatt catttacatt ctactttaag ttcctcctta accattaatt
      961 ttattaaaga tacttgtaat agtgattctc acaaaatttc atgaaaaata ctttattact
     1021 gtatctaaaa ttccaataca caacttatcg actattatct gaaaaccaaa acttattatt
     1081 ccttgatgtt attctagcaa tagtaactgc ttttttattt ttttgcatat tttttcggcg
     1141 ttacttttta aagttaactt tttcgacaaa tagaaagaga tacgaatcac tgattttttt
     1201 gaagaaaaga aatggagtga gctgaacgtt agagatattg ggagctttcg taactgctta
     1261 ttaaacgata atggttattt gcaaccgtat tgctcgcttc tacatgaaat atttcatgaa
     1321 atatttcgca aaacctttga ttatgtagtt acgtttccat tcttgactaa aaatgtttca
     1381 aaaacgaaca aaggtgttaa aaggtaaagt gatcaatact actggtcaaa ataagaggtt
     1441 gaaagggaat tgaaaaataa ctacagataa tacttttata aacgtacgtg ctttgtggtt
     1501 ctgtagttaa ctatataact ggaattaggg aatggtatca atttgaaaag aaagattcta
     1561 ccaatttatc atatgttaaa aatggaaaat caacagcagt tctttcggaa accttacaca
     1621 aaaaccgatt attgaaaata gccgaaaaac gtttttcggc tattttttta attaagtttc
     1681 cttcttctta ccttttccca ctattccgtt tcacatcttt gatgatcttc agcgcacgtt
     1741 ttcgtgtttt gatatttggg cttttcaagc ggcggtatgc acttgagata tctgtttttt
     1801 ccatatggac gacccacttt cttctttgat tgtagtcatg ctaaaaaaca caaatcgaaa
     1861 caattacgat ttgtgtttta aatggtatca aaagttaaaa tgaaagtcaa cttgttaaaa
     1921 cagtcgatca gactagctga ttgactccta tctctggaaa taaattttca ttattacgtt
     1981 ttagctacct aaaaaaggcc ctcttgtccg ttaactttaa tatctattgt tatagctagt
     2041 ttacctattg aacgtactgt gtgttcgata gaacactaaa aaagaggctg ggacagaagc
     2101 gtttaactcc aagaaataag aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt
     2161 tcagcttatt tccgaaggag ttgcttctgc ttccgccgtt tatacgtgtt tagagcgtga
     2221 gacaaaagtg ttctttactt ttgtctcacg ctcttttaaa ctatttcgaa gtactaaact
     2281 tatgaagtaa gaatacctaa gccagagcca atgtgctcgc ctgcaagcaa ttgctctttt
     2341 gcttgtcgct gtaaactcgt actttctgga taatcatgta tttgggcatc tactcgtcca
     2401 aagatccaag ggactccact atcgtcctgt tcatcaactg ataataatcg ataatcggct
     2461 gcaaaaagac agatttttga ctcttttttc aaccatttag taagattggg tgatagtaac
     2521 caagtatctg tgtacatcac ttggtaatgg taatcaggaa aataaagttc aaaaaaacga
     2581 atggcacttt cgtaactatc tcttatctta ttctgagata aatctgcatc ggaaggaata
     2641 tgaagagaaa ggcactgctt tccttctaat cttagatccg tgtacccttt cggaaaatat
     2701 gtcatttcgt actccaattc accaatccga aagatcaagc cactagtgta atgccaaatc
     2761 caaaaaccac ggtcaaagcg atagcttcct gttacttgga aatgttcatg aataaatcta
     2821 gtaaaagcat tcattgttgc caaatagata gttcttggaa tcttcaatgg ttgataaatt
     2881 tttttccaac tttgagaagc tgcatataga taaatagcta atgctgcaaa aggttcctct
     2941 tcatagactt tgtctatttt ctttgcagtc gcctttgctt tattaggatc cattagttca
     3001 tgactatatt gctcaaacac ctcatcaagg atgttttttg cttgtacaat catttgctct
     3061 ataacgactt tgggaagagt taaagtttct gccagaattt ggatttcatt gcttattttc
     3121 ttcatcaaat ttcccctttt catatctttg aacctcattg tttaaatgac tgattatccg
     3181 gacaccatat ggtataaaca
//
LOCUS       DACESX010000182         3183 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.186, whole genome shotgun sequence.
ACCESSION   DACESX010000182 DACESX010000000
VERSION     DACESX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.186"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..461
                     /locus_tag="IXF88_002583"
     CDS             <1..461
                     /locus_tag="IXF88_002583"
                     /inference="COORDINATES: protein motif:HMM:NF025072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1509278.1"
                     /translation="LIKAYQLQNVIFLLGGQSNPYKYMSRADLYVMPSITEGFPNALV
                     EAMSTETAVLCADCKSGPREILSNRTLDTICENIEYAESGILVCPMTDSRNYSPQYEE
                     CDKNLANAIEELIENDNLRNKYAKAAKKRAELYSYDAFEHNLLQIMKTFN"
     gene            499..1590
                     /locus_tag="IXF88_002584"
     CDS             499..1590
                     /locus_tag="IXF88_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1509279.1"
                     /translation="MLKKNTSLQNKTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
     gene            complement(1658..3136)
                     /locus_tag="IXF88_002585"
     CDS             complement(1658..3136)
                     /locus_tag="IXF88_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ1509280.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
BASE COUNT         1014 a          508 c          573 g         1088 t
ORIGIN      
        1 atttaataaa agcatatcaa ctacaaaacg taatattttt gctaggtggc caatcaaatc
       61 cttataaata catgtcacgt gcagatttat atgtcatgcc atctataact gaaggttttc
      121 ctaatgcatt agttgaagca atgtctacag aaactgctgt cttatgtgca gattgcaaat
      181 ctgggccaag agaaatatta tcgaatagaa cgctagacac gatttgtgaa aatatagaat
      241 atgctgaatc aggcatatta gtatgtccaa tgacagacag tagaaattat tcgccccaat
      301 atgaagaatg tgataaaaat ttagcgaatg ctatagaaga attgattgaa aacgataatt
      361 tgaggaataa gtatgcaaaa gcagcaaaaa agagagcaga actatatagt tatgatgcct
      421 ttgaacataa cttgttgcaa attatgaaga catttaatta attcatatga tgaaataaaa
      481 ataagggagt ataactagat gctgaaaaaa aatacatctt tacagaataa aacaatatta
      541 ataactggcg cagccggttt tattggatca aatttggttc ttgagttatt acgtactcag
      601 tcatcaataa aaatcattgg cattgacaat atgaatgatt actatgatgt ctctattaag
      661 aactatagat tatcagagat agaaacggag gcaaagaacc atccaggatc tacttggaat
      721 tttatcaaag gttctattgc tgataaagca acaattgatt tcctctttga taattataaa
      781 ccaagtatcg tggtgaattt agcagctcag gcaggagtcc gttattcaat tactaatcca
      841 gatatgtata tcgaatctaa cataatagga ttctataata ttcttgaagc ttgtcgtcat
      901 tcatatgata atggagaatc tggtgtggaa catttagtgt atgcctcatc gtcttccgtt
      961 tatggtacta ataagaaaat tccttattct acagaggata aagtagacaa tcaagtatcg
     1021 ttgtatgcag caactaagaa aagtaatgaa tcaatggcac atgcatattc taaactttac
     1081 aatattccgt ctacagggct acgtttcttt acagtatatg gtcctgctgg tcgtccagat
     1141 atggcttatt ttggttttac tgataaactt cgtgctggtg ataatatcca aatattcaat
     1201 tatgggaatt gcaagcgtga ctttacctat atagatgata ttgttgaagg agtaaagagg
     1261 gttatgcagt gcgctccaga gaaaattgat ggggaagatg ggctaccaat cccaccatat
     1321 catatttata atattggtaa caatacgccg gaaaacctac tagattttgt agatattcta
     1381 caacaagagc taattgcagc aggggtgcta ccagaagatt acgactttga atctcatacc
     1441 gaactagttc caatgcaacc aggagatgtg ccagttactt atgcagatac tagtgcgtta
     1501 gaacgagatt ttgaatttaa accaagtaca agtttgcgtg acgggcttcg taaatttgcg
     1561 cagtggtata agtcttttta tgaagtataa ttgtattttt ttacttataa aagtttatag
     1621 aaacacctca gacactacaa atctgaggtg ttatttttta atctcgttga aataaatctc
     1681 tggtatatac tttttctttt acatcatcaa gattactatc gtaacgatta gcaataatac
     1741 tgtgactgat atttttaaaa tcatctaaat tatttattac ttttgaacca aaaaatgtag
     1801 tgttattttc aagtgttggt tcataaatta caatagttgc cccctttgct ttaattcgct
     1861 tcataactcc ttgaatagat gactgacgga agttgtctga attactcttc attgtaagac
     1921 ggtatacacc tatgactacc tctttttctt tttctgcatt ccacgaactg ttagcctcat
     1981 acgtgccagc aagttctaac actcgatctg ctataaagtc ttttcttgta cgattactat
     2041 cgacaattgc acttatcata ttctgaggaa catcgttata gttagtgaga agttgttttg
     2101 tatctttcgg taaacaatag cctccatatc caaaagatgg attattataa tgtgtgccaa
     2161 ttcttggatc taagcaaacc ccgtgaatga tttcttgtgt atttagtcct ttcatttctg
     2221 cataagtatc cagctcattg aagtaagata ctcgaagtgc aaggtaagta ttagcgaata
     2281 atttaactgc ttcagcttct gtgaatccca tgaaaagagt ttcgatatct tttttgattg
     2341 ctccttcttg gagtaaatct gcaaaagtgt gagaggcttc tcgtaaagtt ttgtcagttt
     2401 ggtccgtgcc gacaataatt cgcgaaggat atagattgtc atataatgcc ttagattctc
     2461 tgagaaattc tgggctgaat agaatatttt tagtattgta ttttttacga atactttcgg
     2521 tatagcctac gggtaccgtc gacttgatga ccattattgc ctttggattg acttctaaca
     2581 ccaatttgat tactgactct acagcagaag tatcaaaata attttgttta ctatcataat
     2641 ttgttggagc agcaatgaca acaaaatcgg cttctctata ggcggctgca ccatcagtcg
     2701 ttgcaattaa attaagattt ttttcagcta gatatttttc aatgtaatcg tcttgtattg
     2761 gagatttctt attgttaatt tgttttactt tttcaggaag aatgtctact gctgttacct
     2821 gattgtgctg ggcaagaaga gtagcaatgg aaagacctac gtaaccagta ccggctacag
     2881 caatattctt cgactgatgt tttgttctgt ttgatttatt tgattcattt ataaatagat
     2941 ctgttggttc aaaatttaga acttctgcta atgattctaa ttgttgtatt gacgggataa
     3001 aatcgagaga ttctactcga ctaattaagg cacggttgat accagtagct actgcaagtt
     3061 gttgttgtgt cattttcaga gattttctag aatcaataat gatttgagct aaacgttttt
     3121 gagataactt tttcatgatg cactcctttt tttgttgcta ataataacaa acaatttttt
     3181 ctt
//
LOCUS       DACESX010000183         3182 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.187, whole genome shotgun sequence.
ACCESSION   DACESX010000183 DACESX010000000
VERSION     DACESX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.187"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            649..744
                     /locus_tag="IXF88_002586"
     CDS             649..744
                     /locus_tag="IXF88_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1509281.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1046..1291)
                     /locus_tag="IXF88_002587"
     CDS             complement(1046..1291)
                     /locus_tag="IXF88_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509282.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1636..1920
                     /locus_tag="IXF88_002588"
     CDS             1636..1920
                     /locus_tag="IXF88_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509283.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1923..2339
                     /locus_tag="IXF88_002589"
     CDS             1923..2339
                     /locus_tag="IXF88_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509284.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2476..3153)
                     /locus_tag="IXF88_002590"
     CDS             complement(2476..3153)
                     /locus_tag="IXF88_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1509285.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT         1056 a          597 c          496 g         1033 t
ORIGIN      
        1 ttccgttttt tcatccattc ctttcttccc cccttacaaa taaataataa cctttaacaa
       61 ccaactcgta aataggttgg ttgttattat acaaaaggat attagtactc agataaaaag
      121 ttgttagtac tcagataaaa agttgttagt actcagataa aaagttgtta gtacccttct
      181 gtatccgttg ggagagtaag gctcaactgc cccctaaaaa gggttctaaa aaatctttaa
      241 aaaataaata aaataaacgc gcgcgcgcga gcaaaatttc ccgccaaaaa atcacccact
      301 ttttaaaata aacaggttcg cgcatgatat taaactgcca atctagctcc ctatggtcgc
      361 tgtaagaagg agtgatcgct acgctctcac attttacttg cagcagtcgc ctttaggctc
      421 cttcgccaaa aataattcat tagggactcc tttgtggcgt cctgagagct cgtctaagcc
      481 gttctaagac aagattcgat aaaaactatg tccttcgttc taaagcgcct tacaggcggt
      541 cagggcaagc cctaacaacc ccaaggcaat aaaaaactta ggcttatttt tgctttttta
      601 ttgtataatt gattgtcgcc tatccgacag tcttgcgact ggaaggaggt gtgtttcgtg
      661 ttcactaact ttatttgctc ccttcttgtt gggcttttaa gcgctttgtt cagttactgg
      721 ttgaataacc gaaacaagaa gtaaaactta attaaggcga ctcagcccac aaaaaaagcc
      781 ccagagaatc cgacctctct ggggctttcg tgtgtttcgt gttcacttat ttgcaatcaa
      841 attataacag aaataaatat ttatttcaaa aatatcctac aaaagtctaa aatccgtaat
      901 tatttcttat atgcaacatt tacaaataaa gtcgctctga gagctcgtat ttttcgattt
      961 aagacgtttt atttagtttt tggtgtattt cattggtcca agctaaaaaa ctcttagaag
     1021 cgattttaga gccatttaaa cccacttaat tatattctcc atgaaattcg ctccatgcgt
     1081 caaaagctgc gtctgaaatt ccgccgtctt cgttcactag ttgcattaat ttttccaacg
     1141 atcgttttct ttcgggtgtc atatttttat attccaacaa tttttcgtct gttaaatcga
     1201 acttgattgc ttgaattgct tgctctaatt tttctaagtt ttgaaaatat tcgtaaggaa
     1261 atttttccat tttttctttc tccagtctca caatttttct agaaataaaa tcacttcttt
     1321 gccgtctata ctctcaaaaa aaatgtcgtt tgaaaaacta aataaatttt tgatcgtgca
     1381 tttttaaatt tggttatcga ttacacgtgc agcactcccc cccgaatttt aaagagcata
     1441 aggaaacgtg tacccacttt ctaaaaaaag tgggtagata ggctgtgcct atcacgtttc
     1501 tcagaaaaaa atttgatatc actttttcgc catcgtgggt agcttatgta tagcattttg
     1561 gcaaaaaaca gctatacaaa tatagtattt gtatggtata cataaattat acataattgc
     1621 aaaaggagat tttttatgga cattttagtt cggaatattg atccaaagtt tgttgcaaca
     1681 atagacaaac gttgccaaga actcacgaat aaaacaggag aaaaatggag tcgaaataaa
     1741 tatctaaaag ttttaatcga aagtgatttt gaccgtcaac ttacgactta caaaaaagac
     1801 caattcgatt tattgctcga taaatttatt gctatccaaa cagaaaatac tcaaatatta
     1861 agcgagtatg tctacacaaa taatcaaatc attgaaatgc ttatcggagg aatgatataa
     1921 ccatgagaaa aacttctgtc caaaaacatg ttactttgta ccctagtcat gttgacgttt
     1981 tagattccat catcgaaaaa aatccagata tcaataacta ctcggaagcc attcgctttt
     2041 tatgtatgag ttatgaaccc gataccaaca cgaccactac cagcaatcag gcaaaactaa
     2101 atgctatggg aaaagaaatt tccatcatga ctgaaatcat cgcagaattt gctgatttat
     2161 atctaaaaga tacaggcgtt cttattggca caaattctaa agtatatcaa gaagtgaaag
     2221 aaaaaatcga agctaaacta aatccatcaa atcgaaatcg accaaagaat catcaaaaag
     2281 aaaccagtca aacagtcact tctagaaatt tcgacccaac aaattttcta aaacgataaa
     2341 aaacagatag gagttattcc tatctgtttt ttatttagaa gccccataca atatgctaga
     2401 atgtactagc aatcttagtc ggttgtaagg gacttgtcta cttgcttaat gtaagggggg
     2461 cgagttcttt tttatttatt cttctaacca gttacgcaga gaaatctttg gcaaatctga
     2521 cgttaggctt gaaaaagtaa attctaattt ggcgatttta ttgccctttt ttgcttttat
     2581 ttttgtgact ttaagatcat tgaagtattt ttttagttct cgtaatgcag gatttaatac
     2641 tttttgatca atgttaccca tttgatagta ttctgggata tccattaagc tcctaaactc
     2701 ttcaatacga actacataat aaccagtaga acggtactgc ataagcagtc ggaatagagt
     2761 ttttgcatac gtagaacgca acgacgtgaa agcagataat tcaaatctac taaattcagc
     2821 tgttaaacca ttaataatat attctaggtc cggattaaca ctgacttcaa caaacttttt
     2881 tgttttattg atttcaaaac cagtaaacaa aacaaagtag cgaatagaat cctcatcttc
     2941 agtacggtaa gtaagattaa gcatatcttt atagagcttt tctaaatcac cgacaaaggc
     3001 ttttgtagca gtcatcttat aatcacttaa ctcttttaat tcctcgaaag aaaatctaac
     3061 ttttttcaaa ccacgatctt tcatacgagc gcataatgca aaaaataaat ccatctgctt
     3121 agcattgaaa tttttcagcg gtactaaatt caattcattt tgatatttga ctgttaattc
     3181 cg
//
LOCUS       DACESX010000184         3134 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.188, whole genome shotgun sequence.
ACCESSION   DACESX010000184 DACESX010000000
VERSION     DACESX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.188"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            178..408
                     /locus_tag="IXF88_002591"
     CDS             178..408
                     /locus_tag="IXF88_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ1509286.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            505..1806
                     /gene="murA"
                     /locus_tag="IXF88_002592"
     CDS             505..1806
                     /gene="murA"
                     /locus_tag="IXF88_002592"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1509287.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1819..1995
                     /locus_tag="IXF88_002593"
     CDS             1819..1995
                     /locus_tag="IXF88_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1509288.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2130..2426
                     /gene="yidD"
                     /locus_tag="IXF88_002594"
     CDS             2130..2426
                     /gene="yidD"
                     /locus_tag="IXF88_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1509289.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2611..3078
                     /locus_tag="IXF88_002595"
     CDS             2611..3078
                     /locus_tag="IXF88_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1509290.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
BASE COUNT         1055 a          515 c          653 g          911 t
ORIGIN      
        1 gggactgcga caagactttt gtctcagtcc ctttttttgc atatttttag catttatttc
       61 ctaaaaaatg taaggggatt cctatatttt taagtatact tgtttttcct tttatacatt
      121 ttatggtatt atcattttat tagttattat aaaacaatcg ttagaaagga cgtcattatg
      181 caagtatacg gaattgatgc ggtgcttcga atcgtgagtc atttggcgtt tatctactta
      241 gccttctggt ctttacgttc gttacgcatt gaaacttttt tcagaccaat gcataatacg
      301 caaattagaa tggcaatcgt acttttttca atttttttag ggtacactgc aagtaatttt
      361 ttcttagaag ttatcgcttt atgccgaaat cttttcgtta gcctgtaaat tttaaaaaaa
      421 ggtactattt taaaagcaag gatgttataa tagaaaagat tttgagtaag tgtatgctca
      481 ttggaatttt ttggagggaa caacatggaa gagatcatcg taagaggtgg caatcaatta
      541 aatggaacag taagaattga aggagctaaa aatgctgttt tgcctatttt agctgctagt
      601 ttgttagcag aagaaggtat tacgacatta gacaacgttc cgattctttc tgatgtcttc
      661 actatgaatc aagtgattcg tcacttaaac gtagatgtag attttgatga acaaaagaat
      721 caagtaacaa tcgacgcttc aagacaatta gaaatcgaag cgccttatga gtatgttagt
      781 caaatgcgtg cttcaatcgt tgtgatgggt ccgctacttg ccagaaatgg acatgcaaaa
      841 gtagctatgc ctggtggatg tgcgattggt aaacgaccaa tcgatttgca tttaaaagga
      901 tttcaagctt taggagcaaa aattattcaa aaaaacggct atatcgaagc aattgcggat
      961 gaattgatcg gtaacacgat ttatttagat ttccctagtg taggtgctac tcaaaatatc
     1021 atgatggctg ccgtaaaagc aaaaggaaca accatcattg aaaacgtagc tcgcgaacca
     1081 gaaatcgttg atttagcaaa cattttgaat aaaatgggcg ctcaagtcta tggagctggt
     1141 actgaaacaa tgcgtattga aggcgtggat catttacatg cagtcaacca ctcaatcgta
     1201 caagatcgta tcgaagcagg tacctttatg gttgctgctg caatgacaca aggcaatgtg
     1261 ctgattgcag atgctatctc tgaacataac cgcccattga tttctaaatt gatcgaaatg
     1321 ggtgcagaaa tcattgaaga agaaggcgga gtccgtgtca tcggaccaaa acatatctta
     1381 cctacagatg taaaaacgat gcctcatcct ggtttcccta cagatatgca agctcaaatg
     1441 acagctatcc aattagttgc agaaggtaca agtgtagtga ctgaaacagt gtttgaaaat
     1501 cgtttccaac atttagaaga gatgcgccgt atgaacgctc atgtcaaaat cgacggaaat
     1561 gtagcaatca tggatggaaa ccatgaattg caaggtgcag aagtatatgc aacagacttg
     1621 cgggcagcag cagcattagt tttagctggc ttgaaagcaa atggaatcac acgtgtgcgt
     1681 aacttgaatt atctagaccg tggatattat aatttccata tcaaactaca gcaattaggt
     1741 gcagacgttg aacgtgtaga catggatcaa acttctgctg aaaaaacagc acaaacgatt
     1801 gcttaaggaa gtggctgaat gagtaaccaa cgatatatct tattaacttt gataaagatt
     1861 ttaatggtga tcttattgct tatccttctt tttgtggccg gaacgatgat cggatatggt
     1921 gtcatcggtg gcggcaatcc atttaaagta tttcagccaa gtttgtggat tcatattcga
     1981 gacttttttc attgataaat aagaatcaca gtggttctag tcgatatgaa gtgagccatg
     2041 aagcagaaca tgataaaaga aaacagaagg gaacgttgac gttagtcgcg atcccttttt
     2101 ttagtaaaag aaaagaagga atctaattga tgaagaagat attgatttat ttagttagag
     2161 gatatcagcg ctttatttcg cctgcatttc cacctagctg ccgttattac ccaacctgtt
     2221 ccaattatat gataaaagca attcaaaccc atggcgcgct aaaagggacg gtaatgggga
     2281 ctgcacggat cttacgctgt catccttttg tcaaaggcgg tatcgattat gtaccattga
     2341 cattccgatt aacgcgaaat aagaaagata aaaaagaacc agattataaa aaggttgtga
     2401 aaaatacggg tagcactgag cgataaagaa acaatttgga aaatagcttc ttatattttg
     2461 caaaattgtg agttattgcc gaagtgttgg attttgaaca ccgtcaaaaa aaaggttgtg
     2521 aaaaatacgg ctagcactga gcgataaaga aaaagaggaa atttattgtc aaagtaacaa
     2581 ctccgcaact acttatatgg gggacaaaaa atgatcgaag aactaaacaa gaaagattta
     2641 ccatggtcct tgttattaga tgcagatcct gataaagaaa aagttcaagt atatgtttcc
     2701 agagggagtg gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact gatctatgtg
     2761 gcaagggaaa aagaatacga aatcgtcaat gtagcagtaa ccccaacgta tcaaggaaag
     2821 gggattggcg gaaaattact tgagacagct tttcagaagc tttcacaact cgcttccagt
     2881 caaactcaga tcatcatacg aaccggcagt actagcagtg cggcgttaca tctctatcaa
     2941 aaaatgggct atgtggagat tggtcgagtc aaagactatt ttatccatca ttactctgag
     3001 cccatttttg aaaatggaga tcaattaagg gatcaagtga ttttagcaat tcacttacct
     3061 aaaaatagct ttaactaata aaatttcaat tcaagaaaat gctttcaaat gctaaataga
     3121 tacattacaa tagc
//
LOCUS       DACESX010000185         3133 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.189, whole genome shotgun sequence.
ACCESSION   DACESX010000185 DACESX010000000
VERSION     DACESX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.189"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            13..1605
                     /locus_tag="IXF88_002596"
     CDS             13..1605
                     /locus_tag="IXF88_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1509291.1"
                     /translation="MLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKEKVSLK
                     MMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLPDAIFN
                     AAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIYSMPWI
                     EELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGNGEADE
                     IPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKYFNEMN
                     NKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGPDGTKH
                     VTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQANIFEL
                     KDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMHDTYLP
                     YITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYLAELER
                     VGLEEWIQIKQTGYDNFLKGAQ"
     gene            1606..3090
                     /locus_tag="IXF88_002597"
     CDS             1606..3090
                     /locus_tag="IXF88_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ1509292.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
BASE COUNT         1017 a          504 c          725 g          887 t
ORIGIN      
        1 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
       61 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
      121 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
      181 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
      241 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
      301 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
      361 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
      421 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
      481 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
      541 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
      601 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
      661 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
      721 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
      781 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
      841 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
      901 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
      961 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     1021 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     1081 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     1141 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     1201 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     1261 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     1321 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     1381 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     1441 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     1501 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     1561 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     1621 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     1681 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     1741 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     1801 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     1861 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     1921 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     1981 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     2041 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     2101 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     2161 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     2221 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     2281 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     2341 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     2401 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     2461 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     2521 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     2581 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     2641 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     2701 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     2761 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     2821 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     2881 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     2941 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     3001 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     3061 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     3121 atttaatagg ttt
//
LOCUS       DACESX010000186         3054 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.190, whole genome shotgun sequence.
ACCESSION   DACESX010000186 DACESX010000000
VERSION     DACESX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.190"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            63..413
                     /locus_tag="IXF88_002598"
     CDS             63..413
                     /locus_tag="IXF88_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509293.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            618..1466
                     /locus_tag="IXF88_002599"
     CDS             618..1466
                     /locus_tag="IXF88_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAQ1509294.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            1513..1707
                     /locus_tag="IXF88_002600"
     CDS             1513..1707
                     /locus_tag="IXF88_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1509295.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            1710..2912
                     /locus_tag="IXF88_002601"
     CDS             1710..2912
                     /locus_tag="IXF88_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1509296.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
BASE COUNT         1119 a          541 c          570 g          824 t
ORIGIN      
        1 gatggccatc tatatttttt aatcaaatcg tataaacttt cgtataaaaa aggagaattg
       61 tgatgccaat aaaaattacc gatctcagtt taccaaaaga aacactcgaa aaagaatacc
      121 aagtcgtcaa tgtcactcgc tggcaaaagg agggcgaaat tctaggttgg agctacgaat
      181 gtatcctacc aaaacttcgc tacgaaaaaa tcagtgtcaa agtcaaatct gaacatcctg
      241 tgatcacgcc ggaagaactt acccaacaag gtttagccat aattacattt accaatttgg
      301 tgattgtacc ttggggacgc tcgaatggac aatttgtcag ttatgggtta tcggcaaccg
      361 ctgatacggc aactttgatt cccagaaaac aaggtaccac tacacaaaac taactatact
      421 atggagtgtt gggtggttag gacgaaacgg cggggcagcg cccacagagc cgtttctgtc
      481 cgttaagacc gcccaacacc ccataggcta gaagggctgc tacgcaggcc ttccccctta
      541 acttgattta tagatcaaca tcaggagaaa atgactggtt ctttgacaat cgaatacgca
      601 acaaggagtg atgatttttg caagcttata atcagaaaat cattgaaact cccacgtata
      661 ttgaaattta tgattatgaa gcaatcatta ctcccaaacg taaaacgatc gaaaacgaag
      721 taaatctgga acgaaaacaa aaattagctt ggatttatga aaatgatatt actcttgaaa
      781 aaagaaaaaa ctatgatgaa ttatccgctc aaggacaata tgacagttta aaacgcaaac
      841 agaaacatta tcaaagtatg cgatttgaga ttgcccgcct tgtagacacg aattttgaca
      901 atcaaacgaa atttttaact ctcactttca aagaaaacat tcaagacatt gagtatgcaa
      961 ataatgaatt taaaaaattt atcaaacgac tgaattatga cctctataaa actaaaaaag
     1021 caaatttaaa gtatctcgcc acgtgggaaa agcagcaacg cggtgcaatc cactaccata
     1081 ttatcttgtt tgatttcccc tttatctcat ttaaccggtt aactacgatt tggggtcagg
     1141 gtttcgttaa gatcaacaag atcgatgtag acagtatcga aaatcgcggg cgctatatca
     1201 gcaaatattt cgataaagag ctggaattga aagagcataa gaaaaaagct tttttcaagt
     1261 cccggaatct caaactacca actgtcacca aaaagatggt aaagaaaccc tatgattcca
     1321 cgaatcaaga tatactatat acaagcgaat atatagttag acgtccattt ttttacaatg
     1381 ctttagcttt aaatgagttg ggaagtcctg aacaagtgat ggattttcaa gaaagtaccg
     1441 ttcattatac aaaaataaag aaataacact attacccaat gatagtgttt tctttgaaag
     1501 tgagtttata caatgaaaaa tacaatttgt cgaaaagatt tagaggctct aggatttaaa
     1561 acacatcaag ctaccacaat aatccgacaa gcaaaaacaa gaatggtaaa tcagggatat
     1621 ccatattata ataataaaag agttggggta gtaccccgac aagtaattga agaaattata
     1681 ggtgttcctc tttcacagga ggaataacta tgaaaacaaa atacccttgt gtgtatcaag
     1741 ataaaaatgg tcgcttttct tatctagttg agttaggaac agatcaagtg actggaaaga
     1801 ggatccagaa gaaaggaaca aaagattctc taggaaaacg attctcatca gctcgtgaag
     1861 cacacaaaga agtcatgcgt ataaaaaatg aatacatggt tgtaaatgga tttgcgaatt
     1921 atgatttaac ttacaaagaa tttatggagc agtcttacgt tccacactac aaagctagcg
     1981 tacaaaaaag tacttggagt tctcgtgagt gtggattgat tcagattact gaatttttcg
     2041 gtgataagaa gcttcgtgaa atcaatgtgc gagattgtga aaattatcga atttggttat
     2101 tgaacgatag cggatactca caagcataca gttcacttat gtacggtatg tttcgtaaat
     2161 cattagacta tgcggtcacc ctgcaatttc tgaatgagaa tatttccaaa agaacaaaag
     2221 caattccaaa aggaaaatca atcgttcctt actggacaaa agaagaattt gaagaagttt
     2281 tggcaaaaat ttataaagat gatttctacg aacatatgtg ttttttaatg atctggctat
     2341 attacatgac tggtgttcgt gtaagcgaag gattagcact taattggaat gacgtggact
     2401 tcaaacgtaa aaagttgagg gttcatcaca ccttggaaat gacaaatcaa aaagatttta
     2461 ttcggaaacc ttacacaaaa actgaaagcg gcttgcggat catttctctg gacgatgata
     2521 caattgaagt gctaaaaaag tggaaagtta ttcaaaaaaa tcatggtgta gaacaattta
     2581 ttcttagcta cactgacctc ccactctatc gttcaactgt tcaacgaatt attcagcgtt
     2641 atgccaaact agctaaggta cccctaatac aaggaaaagg attacgtcat tctcatgtta
     2701 gctacctaat caatgagttt aacgcagaca ttctggtcgt gtcgcaacgg ttaggccatt
     2761 ctagccctga aatcacttta aagcactacg ctcatctatg gagccgtaat gatgaaggaa
     2821 ttgcagaaca aatgacagga aacattaaat ttacctgtgc aacaaaatct aaagttgcaa
     2881 actttaatgg aaatcaagca gtaaaaatct aaccctacca tttccctacc aaaatgagga
     2941 ataggctgaa aaagcactga tataatagct tctttacgag gctactcata ttgagtggga
     3001 ataagacgta aatataatat ggatggggaa tgccgataaa tcaacgtttt tcaa
//
LOCUS       DACESX010000187         2990 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.191, whole genome shotgun sequence.
ACCESSION   DACESX010000187 DACESX010000000
VERSION     DACESX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.191"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            43..777
                     /locus_tag="IXF88_002602"
     CDS             43..777
                     /locus_tag="IXF88_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1509297.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            793..1962
                     /locus_tag="IXF88_002603"
     CDS             793..1962
                     /locus_tag="IXF88_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1509298.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            1978..2973
                     /gene="lacD"
                     /locus_tag="IXF88_002604"
     CDS             1978..2973
                     /gene="lacD"
                     /locus_tag="IXF88_002604"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1509299.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
BASE COUNT          962 a          553 c          679 g          796 t
ORIGIN      
        1 ctatacttac attgtggtta tagccagtta ggagagacat cgatgcaaaa tacctttcaa
       61 tcaaaatcac tttattatca gctggtagaa atattgaaac aacgaatcga aaacagtatg
      121 gtcgctcatg acaagctgcc atcagaaaga gagttgacca gtttttatgg cgttagtcgc
      181 acgacggtgc gactagcatt gcaagaactg gaaaaaatgg gctacattta tcgacaacat
      241 ggaaaaggaa cctttgtttc ggaattaagg acaaatgcga ctgatctagc tggcgcatat
      301 agttttaccg aacaaatgcg ggcaatgggc aaacatcctg aaaccaagat attggccttc
      361 aagaagattg aagcaactaa atatttagcg agtcatctga atgttcaact aggtgagcct
      421 ttatttcaat tagaccgatt gcgcttagct gatgatttgc caatgatgat cgagaaatcc
      481 tatttgccag tgaagctatt tatgtccctt tctcaagaaa ttctatttct gaaaccttta
      541 tataatttgt tttcagagaa tttcaatcaa atcgtacggt tggctgacga ggaattttat
      601 gccagcattg tccagaaaga agatagtgag ttgctaaata ttccagaagg atcaccagtg
      661 ctccatttag ttcgctcaac ctataatctc gagaacgaag tgattgaatt tacattaagt
      721 gtagcgcggg ctgataaatt tcattataag atcaggcatc tgcgttccac taactgaatt
      781 ggaggaaaga aaatgtttat gttctcaaaa gaagaactac gaaagctagg agcagaaaat
      841 actacccgag aaattcgaca gcaaccggaa ctgtggcatg agacgtttga ccgttatcaa
      901 gaaacagttg cagatttaga tcgtttgttt acggatgcga aacaacatgc tggagagcga
      961 ccaatccgag tcatttttac aggggccgga acctcgcaat atgtgggaga tacactaatt
     1021 ccgtttctta ctagtcaagg aaatacgaac cagtttattt ttcaaagtat tggttcgacc
     1081 gatatcgttg ctacgccaaa agaatacctg attcctgatg agacaacgat cttggtctca
     1141 tttgcccgta gtggaaatag tccagaaagt ctagccgcag ttaagatggc caatcagctg
     1201 gtcaacaaga tctatcatat cgtgatcacc tgtgcaccag acggattatt ggctacacaa
     1261 atgcctacaa aaaaagaaaa tctagtttta ctaatgccaa acaaagccaa tgacgctggt
     1321 tttgctatga caagcagttt cagctgtatg acgctgactg cgctacttat ttttgatcaa
     1381 gcaagcaatc agcaaaaaga aagatatgtt catcaattaa gccgactagg agaagaagtc
     1441 atccaacgag aatcagagat ccaagggtat ctggcatcag actttcatcg catcatttac
     1501 ttaggttcgg gaagcttagg cggattgact agagaagcgc aattaaaagt tctcgagtta
     1561 actgctggaa aaatcgcaac cgtatttgat acatctttag gattcagaca tggtcccaaa
     1621 tcttttgtca atgagcaaac attggttttc ctgtttgtca gcaatgactc atatacgcag
     1681 aaatacgatg tagacatgct ggaagaaatc aaaaatgacg gtattgccca acgcgttgtt
     1741 ggaattggtc aaaagggttc attgtttagt ggtgattcct ttgaattagt tgcagattcg
     1801 ttactgccgg atggctatct tgctttagca gatatcatgg tagcccaaac gatcgcatta
     1861 ctttgttcgg tgaaggttgg taatacacca gatacaccat cgccaagtgg aacagtcaat
     1921 cgtgtggtca aaggcgtaac gatttatcca tatgaaaaat aggaaagagt gtgaagcatg
     1981 ttagaatggt cggaaaataa aagacaagca atggaacgtc tatcgacgcc agaaggcatt
     2041 attaccgctt tggcgatcga tcagcgaggc gcgctgaaga aaatgatgtc ggctttaggg
     2101 atggaagcga caaaggaaaa aattgagcag tttaaaacac taatttccca agaattgact
     2161 ccttatgcct cagcaatttt attggatcct gaatacggac tgcctgctgc acagaagcgt
     2221 gcagcagcga gcggtctgct gttggcatat gaaaaaactg gttatgatca aaccgtaccc
     2281 ggccgcttgc cggatctgct gtcggactgt tcagttctgc gattgaaaga gttaggtgcg
     2341 gatgcggtga aatttttact gtattacgat tatgaagaag cagaggaaat caatcggcaa
     2401 aagcaagttt ttgttgaacg aattggaagc gaatgcatgg ctgaaggact gccattttat
     2461 ttagagttat tgtcatatga tgctaaaaat gtagatacga aatcgctttc ctatgccaaa
     2521 gcaaaaccgc gcaaagtgat cgaaatgatg aaagaattct cgaaaaccca gtataaagta
     2581 gatgtcttaa aggttgaagt accagtaaac atgtcatttg ttgaaggaaa tgcaacaggg
     2641 gagatcgcct atacacgtca agaagctgcc gcttatttta aagaacaaag cgatgcaaca
     2701 gatttgccgt atatcttctt aagtgctggg gtatcagcgg cattattcat ggagacattg
     2761 cgttttgcca aacatgctgg ggcacgattc aacggtgtac tctgtggcag agcaacttgg
     2821 aaagatgcag tcgctccttt cgttgaaaaa ggagaagcgg caactttaga atggctgaca
     2881 gaacaaggga cacaaaacat tcgtcaattg aatgaagtga taagagaaac tgccatccca
     2941 tggtatgaga aagtaagtga aagcacttgt taatgtttct aatatattag
//
LOCUS       DACESX010000188         2957 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.192, whole genome shotgun sequence.
ACCESSION   DACESX010000188 DACESX010000000
VERSION     DACESX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.192"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(42..593)
                     /locus_tag="IXF88_002605"
     CDS             complement(42..593)
                     /locus_tag="IXF88_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ1509300.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(767..1066)
                     /locus_tag="IXF88_002606"
     CDS             complement(767..1066)
                     /locus_tag="IXF88_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ1509301.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(1093..1359)
                     /locus_tag="IXF88_002607"
     CDS             complement(1093..1359)
                     /locus_tag="IXF88_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1509302.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            1646..2389
                     /locus_tag="IXF88_002608"
     CDS             1646..2389
                     /locus_tag="IXF88_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509303.1"
                     /translation="MVVSKLPRNEDYLTNYDGYVVTTEHFDLANPVTKVDDIPDYEMY
                     SQTIDSLNKRFGNRVLKGIKIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDD
                     EVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPIL
                     DQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFD
                     DLFALLKANGVTELAIYQKGKRIMVPLPV"
     gene            complement(2426..2923)
                     /locus_tag="IXF88_002609"
     CDS             complement(2426..2923)
                     /locus_tag="IXF88_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1509304.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          893 a          560 c          453 g         1051 t
ORIGIN      
        1 gcatgtaaaa ctacttataa gtaacatgag aaaataaaat gttatttttc ttgaaataat
       61 aattgactat ctgccaattg ccattcatta cgaacatgaa tacttagtgg gtgggataac
      121 ttaccttctg catttatcaa tactttggtc atcttctttt gtttcgcact caattgatcg
      181 taaatagtct ttgcttgttc ataagccact tgactcgtcg ctgtaatcga accacaagca
      241 aaaataatgg tattgatttt cttttctgta aaaacagtcg ttaatatttc caaagattgt
      301 tcatcatttc ctcgaagaaa tacccgagga cttttcgctt ttgtgtacga aaaaagattc
      361 acagcaataa aaccagtaaa tcctaattgt cggatattct tttcgattaa taacatactc
      421 aaatcattcg taaatggttc tgtatctgtt ggacgaatag taatgactac tgctaattta
      481 tcttccgctt cttttccttt gatcgcccat tgtcgtttga aaacatagcg atgggtatga
      541 tgaggatctc gccaaacttc tgtttgtgtg gtcactaatt ctttttcaat catctctttt
      601 tctcctatgc tattttttgt tcaagcactt caattaccat tgccgttata agtgtcaata
      661 ctagttgaaa atatttcata ttctttctcc ttttttcttc agaatttgat gaatcgcggt
      721 aaggatatct tctaaacatt tcgcaatcat tctcaaataa tcgatcttat tcatccgttc
      781 ctccaatctg actccaaacc aatcgtcctt ctaccagtaa cccacgaaca aattctcgct
      841 ggctggtaat atctgtaatg aaataaacta catagtcttt tccatcttct ggtgcgcaat
      901 aaatataaag atgtgtcatc actaaacgtg gtagatgaac atcttcagga agcataccca
      961 tttcaatttc ctctttttcc atcgtaacgg attcatagcc ccactcttca gaagcagtga
     1021 ccagctgtgc acacttttcc agtacttcga tagccgtttc taacaagtcc tttcgttcct
     1081 ttcttttaga tactagtatt cttctggtaa taacatcgtt tgatactcac ccgaatcaat
     1141 ggcccatatt ttttgagaca ctgatttcaa acgaagctta ggtgaaatca gatactcttt
     1201 tctttcttcc ggttcttctt gacgatgaac caacttttga tttccttttt ccgtagaaaa
     1261 ttcaaaaatt tgaagatagt ctaaaggaaa ttgcttttga acgttttgat cgattagatt
     1321 ccaacataaa gcttgtaatt ccaccggcaa actgtccatt actccctttg tgacataacg
     1381 ttatccttta ttttgaaaca tttcttttcc ctcctttgcc ataaaaatag cccaatcttc
     1441 tattaaagaa gattgggctt catggatata atatatattt gtaaataaag ttaattcagt
     1501 tataattatc aaaactacac aattattaaa gtttttcata tctctttgtt tagtaagttt
     1561 ttctaaatct taacccgtgg tgctagttaa ttttttccta gaggaaaatg tctaaacatg
     1621 ttggtaaatc aacaaaaaaa cgcatatagt agtctcaaaa ctcccaagaa atgaggacta
     1681 tttaacaaac tacgatggtt acgtcgtgac aactgaacat tttgacttag ccaatcctgt
     1741 tactaaagtc gatgatatcc ccgattatga aatgtacagt caaacaatcg atagcttaaa
     1801 taagcgtttt ggcaatcgtg ttttaaaagg catcaaaatc ggttacatcg cttctgaaaa
     1861 ggatcggatc atcgactatt tagccgataa agattacgat ttaaaattac tcagtgtgca
     1921 tcataatggg caatttgatt atttagatga cgaagtcaaa gatatggatc ctgctattgt
     1981 gattcctcag tattttgcac aactttcaga agcactagtt gttatagaag cagatgtctt
     2041 tgctcatttt gactatggtt ttcgggtatt tggtctatcc gttgctgaat ttaaacaata
     2101 tgaagcgcag tttttaccga ttctagatca agtaatcaaa aataaattgg cattcgaatt
     2161 aaatgctaag agcgcttact tgtatgacaa tttagcgtta tatgaatatg tcatcgattt
     2221 atatctttca cgtggtggta ccttgtttag tgttggatca gatgggcatt accttgaaca
     2281 tttccgtttc catttcgatg atctttttgc tctgttaaaa gcaaatggtg tgacggaatt
     2341 agcgatttat caaaaaggaa agcgaattat ggttccatta ccagtctaaa aaaacgtcct
     2401 cagcatcaaa agtgaggacg tttttttaga tttctttttc taaaatcgga caaatacgat
     2461 cttgttttcc taaaggaaaa tgtttgatcc cgattttttt aaatccatga cgttcataaa
     2521 aacgaatacc tgcatgattt ttttcataaa cagtcaaacg taaataacgt ttttgtttcg
     2581 ttttagcata acgattcgct tcatcgagta accgttgccc taaccctttg ttttgataat
     2641 ctttgagcag atagagccgt tgaatctcta tagcatcttt ttcatcattg actttcatat
     2701 aaccaatgag tatttgatga tccttaatac caataaattt actggttgac tgtgccaatt
     2761 ctactgccaa tatttcagtt gtcaattgtg tctgtaaata tccttgcata tcaagtggat
     2821 ctgcgccatc gataaacgat tcataaaaac tgacaacact tacaacttgt aaagcatcta
     2881 gatcatcata atctaaatac gctagtgtga tttgattgtt catctaactc ctccttttga
     2941 catagtatac tataaat
//
LOCUS       DACESX010000189         2926 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.193, whole genome shotgun sequence.
ACCESSION   DACESX010000189 DACESX010000000
VERSION     DACESX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.193"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            34..1368
                     /locus_tag="IXF88_002610"
     CDS             34..1368
                     /locus_tag="IXF88_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509305.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1355..>2926
                     /locus_tag="IXF88_002611"
     CDS             1355..>2926
                     /locus_tag="IXF88_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509306.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIRLVSKGFKNADFIAI
                     FKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDL
                     TTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLL
                     QFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQK
                     IISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPY
                     KTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVAD
                     IKVSTSYTVTTIISYL"
BASE COUNT          997 a          437 c          503 g          989 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt attcgtttgg tttctaaagg
     1981 tttcaagaat gcagatttta ttgctatatt taaaaaatta tttagtgcgg caggaatgac
     2041 attggttctg acgagtgctt tttggtatcc gatgtttgaa caaatgctct accaaaaaat
     2101 caacaaacct agcgtgacaa atttatatgc tcatgcatct aatgtgttcg atagtctgac
     2161 tgaagctatg aacaacgact taactactta ctcaatggga ctagttggtc tgttatcttt
     2221 atgtattccc ctgattttat ttaaaaaatt aacaaacata gaaaaaaaga tttattatgg
     2281 tacgtgtttg acttggctag ctacaactag tttggtgcca tggtatcttc tgcaaaatac
     2341 gccagccaaa ttgttacaat ttccttggcg gatcttaagc ttgcaaataa tattctcttc
     2401 tctgatatta acgatgatat ttttcaaaaa tagaagatac aataagacaa gagaactatt
     2461 ttatttattt atgactgttt tgttattaat cactttaact gtttctagta aaaaaaattt
     2521 caatcaaaaa ataatttctc agccgggaca tattgttgta aacaaagaaa cagtagaggc
     2581 gtataccact aaaggaatgg attatttttt atatgattat tctcctatcg cttcattgga
     2641 gaataaggag catataataa aacacgaagc aaagattggc agtcaatggg taaaccttcc
     2701 ttataaaaca actggtagtt ctatcatata tagagtaact aatgagaaga gccaagtaat
     2761 cacacttcct gtatatgctt atttaggaac gaatgtagaa ataaatcatc aacctgtaaa
     2821 ggagcaggtg aatgctaatg gattggtaca aataagcgga gtcaaaggag ttgctgatat
     2881 taaagtaagc acaagttata cagtaacgac tattatttct tatctt
//
LOCUS       DACESX010000190         2903 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.194, whole genome shotgun sequence.
ACCESSION   DACESX010000190 DACESX010000000
VERSION     DACESX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.194"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..539)
                     /locus_tag="IXF88_002612"
     CDS             complement(<1..539)
                     /locus_tag="IXF88_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1509307.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQG"
     gene            674..1063
                     /locus_tag="IXF88_002613"
     CDS             674..1063
                     /locus_tag="IXF88_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509308.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1067..1831
                     /locus_tag="IXF88_002614"
     CDS             1067..1831
                     /locus_tag="IXF88_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1509309.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1960..>2903
                     /locus_tag="IXF88_002615"
     CDS             1960..>2903
                     /locus_tag="IXF88_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1509310.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETT"
BASE COUNT          925 a          496 c          605 g          877 t
ORIGIN      
        1 tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata ttttggctca
       61 taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag ttcacgttcg
      121 ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc cattgcagaa
      181 gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt atcttctgcg
      241 ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt gtcttcaatc
      301 aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa attcagtgga
      361 ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat ctcgaccata
      421 ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg taacgtgttc
      481 agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg atcaatcatt
      541 tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag tgaagaaaaa
      601 ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta taaagatagg
      661 ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg tgctaagtct
      721 ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg tagagaacat
      781 tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct ttttgatact
      841 gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta tttatccact
      901 cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc ccgagccatt
      961 cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg ttggagcatg
     1021 gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga atccaattat
     1081 attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag aattaagaga
     1141 ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg agttagtttt
     1201 gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag atgcgatgaa
     1261 tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt gggatagtat
     1321 ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa gaggtcttct
     1381 acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat gcgccttgtt
     1441 ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg cacctttgga
     1501 atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac caaaagacta
     1561 ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg aaaagttagc
     1621 tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt atatacaaac
     1681 ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg tttttcgttc
     1741 ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt ccactttttt
     1801 agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc atgacgatag
     1861 aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat ttggtataat
     1921 tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt agcagatgtt
     1981 gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca tccggaacag
     2041 gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga ttaccgtcca
     2101 aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt atttatactg
     2161 gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat cgctaaacgg
     2221 atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga cgtcggttct
     2281 tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat cgaccgtttg
     2341 gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt agacagcgat
     2401 aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta tcaaacgatt
     2461 atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac tggttattta
     2521 tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa tcattcgaca
     2581 gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac ttgttttgta
     2641 tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc tggaggacac
     2701 ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca aatcagttca
     2761 cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg tggagaaatg
     2821 ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt ttttcctgaa
     2881 ctagttatca gagagacaac gaa
//
LOCUS       DACESX010000191         2883 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.195, whole genome shotgun sequence.
ACCESSION   DACESX010000191 DACESX010000000
VERSION     DACESX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.195"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            573..>2883
                     /gene="esp"
                     /locus_tag="IXF88_002616"
     CDS             573..>2883
                     /gene="esp"
                     /locus_tag="IXF88_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1509311.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHK"
BASE COUNT         1048 a          432 c          563 g          840 t
ORIGIN      
        1 ttataaagaa aaagaatcaa taatttaatg aggctgtgac aaatgtcatt ttttgtttcg
       61 ctaaaaacga gtgaaaaaat gttgataact ccaatcattc gtttaagatt ctaggaatca
      121 ttcaggaatg ctgtgaaacc gatactttat aattataaag aaggtgattt caaattccga
      181 agaaggaatt tgaagtcacc tttttactta tgtcacagtc tcattcagta atgcgggata
      241 aggctcctta ttccgctatt tgaatactct gaagtttaat ctaagaaaga gtaatatttt
      301 gaaaacatgc ttcatcgcat agaaatgaac ttaatttcag aaagaataca tacatatctt
      361 tttatatgtt ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa aaacatgaag
      421 aaatgtactt tttttgttgt aatgtgcatg aaattcttta agtaaactag ttaacataag
      481 tttagtaaga ttcctattct gtgaataata cgtatcagat ttatctttta ttcttactaa
      541 gaaaataact attaagggag ttgatttgaa taatggttag caagaataat aagagagtat
      601 ttctagaaaa aacaaagaaa cgagtattga agtacagtat caaaaaatta agtgttgggg
      661 ttgcatctgt tttggttggt gttggtcttg tcttgggaac aactgaacta gttcaagcac
      721 aagatgaaat ttctccaagc accccacttg aaacagcgat aagctccgta caagtaggtg
      781 acaaagtagc ttcggggaat acttttcagg agaatcctgg ttatacgaaa aattataact
      841 tttcggattt acaatttagt cctcaagaac taactggaga cacactaaaa ggcaatacaa
      901 ttggatttga agtttacgga aaacataata ttgcagcatc aactaaaaac tgggaaatcc
      961 gtcttcaatt agatgaacga ttagctaaat atgtagaaaa aattcaggtc gatccaaaga
     1021 aaggaattgg atcttctaga cggacctttg taagaattaa tgattcgctg ggtagaccta
     1081 caaatatttg gaaggtcaat tatattcgtg caagtgatgg attatttgct ggggctgaaa
     1141 caactgatac acaaactgct cctaatgggg taattacgtt tgaaaagagc ttagatgaaa
     1201 tttttaaaga gataggcata gacaatctca agaccgatcg tttaatgtat cgaatctact
     1261 tggtaagtca tcaagacgat gataaaattg taccaggtat tgatagtacg ggctactttt
     1321 taacagattc agatgatttc tataatagct tagatgtatc tgaaaataat cctgaccagt
     1381 ttaaacatgg atcagtaagt gcaaaatatg aggaacctaa tactcaaaca aaagatggtt
     1441 caggatccac aggtgctaat ggagctataa tcttagatca taagttaacg aaaaattata
     1501 atttctcata ttcagcttcc gcaaagggca cgccttggta tgctaattac aaaattgatg
     1561 aacgattagt tccatatgta gcgggtatac agatgcacat ggttcaggct gacaaagtaa
     1621 catatgatgt ttcttttgaa tcagggaaaa aagtggctga tttagcaatt gaacggcgca
     1681 aagatcatga aaattatggt gtaggttcaa ttactgataa tgatcttacc aaacttattg
     1741 attttgctaa tgcaagtcca cgtccagtcg ttattcgata tgttttacaa ttgactaagc
     1801 cattagatga aatcttagaa gatatgaaag caacagcaca agttgaagaa aataaaccat
     1861 ttggtgaaga tttcatcttt gattcttggt tgtcggatac gaataaaaaa ttaatccaga
     1921 acacttatgg aacaggttat tattatttgc aagatattga tggtgatgga aaccctgacg
     1981 ataaagaaga gagcggagac acgaatccat atatcgggaa acctgaatta gaagaagtat
     2041 atgatgttga cacaacagtt aaggggaaag tattcatcca cgagttagcg ggaacaggtc
     2101 acaaagccca acttgttgat aaagaaggta ctgtattagc agaaaaaact atcgctccaa
     2161 atgaaaaaga tggggctcca atttcagata ctgtagaatt tgaatttacg ggtgtagatt
     2221 caagtaaatt aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca ggttttgata
     2281 aaccagaaga aggttcaacc gttattaagg aatcaccaaa agcggttgat aaacaaaccg
     2341 tggtagttgg atttaaacca gatgctaaag aatcaattcg gaataataaa aacttacctg
     2401 aagatgcaga gtattcatgg aaaacagagc ctgatacttc taacgttact gatagtacga
     2461 aaggtattgt aactgttaag atcggaaatc gaactttcga cgtggatgta gagtttgctg
     2521 taaaagcttc tcaagctatg gaaaatgatg caacatacgt acctataaca acaactccag
     2581 aaacgacagt tcaaagtgga aaacctacat ttgataaacc agatgttcct ttagctaaag
     2641 atgccttttc aattttagat gtctataata aggacttcgg taatgcaagt gttgatgcaa
     2701 atactggtat tgttacattc actccagcta aaggtgtagg agaatcggag ccgattactg
     2761 gaacaattcc tattaaaatt gtttaccaag atggttctgt aggcacgacc gatttagcag
     2821 taactgtaag taaagatatt tatgaaaatc caggagaaaa cattcctgca ggctaccaca
     2881 aag
//
LOCUS       DACESX010000192         2849 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.196, whole genome shotgun sequence.
ACCESSION   DACESX010000192 DACESX010000000
VERSION     DACESX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.196"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            153..575
                     /locus_tag="IXF88_002617"
     CDS             153..575
                     /locus_tag="IXF88_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509312.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            595..2184
                     /locus_tag="IXF88_002618"
     CDS             595..2184
                     /locus_tag="IXF88_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509313.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKSEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            2196..2531
                     /locus_tag="IXF88_002619"
     CDS             2196..2531
                     /locus_tag="IXF88_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509314.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
BASE COUNT         1137 a          423 c          571 g          718 t
ORIGIN      
        1 acaagataaa tttgatcaat tattaaatca gttaatagat aaattttctt ttgttcaaag
       61 tcataataca aaagtattag aagaatatat ttgtcagaat aatcaaatta tcgaactttt
      121 aattgaacaa agtagaggag gagaagttta acatggccaa agttatagac aagcatattt
      181 atttttatga aacacatata gatgttctag atgaaattat tgcaactcat gcagaggtta
      241 aaaactatag tgaagccgtt cggtttctat gtatgaatta tgattcagaa gtggagaaaa
      301 aagtaaattc caatcaagca aagttgaatg caatgggaaa agaaatttct attttaaccg
      361 aaatggtttc agagtttgcg gatctccatt tgaaagatac agggatttta attggtacaa
      421 attcaactgt ttaccaagaa gcaaaagagc gtgtggaagg caagataaaa gcaaatcaaa
      481 cgaagaaata ttctcctaag ataaagaaaa caataagcga atcagtgact acaaaaagtg
      541 aagtatttga tccaacaagg ttcttaaaac gataaaaatt agaaggagaa tgtgatgagt
      601 aaatcacctg cagtcattct cacttgtcaa tttaccttgc ccaataaaaa atctttttca
      661 acatacattg attatatgac aagagaaaaa gcgctggaag aaaaggaacg acgtacgccc
      721 gaggaagaac aggaactgcg tctaatcaag aatgctttag aggattttta tattcctgaa
      781 ggagaaactt tttcggagag taagacgggt aaagaattca gcgaaacgtc gaatgaagcg
      841 caatcgttga tgaaacatgg aatcaatttt gacaagtcag aagaacaaga ttttaccaaa
      901 tatctttctt atatgacacg tcactatgcg ttagaacaaa agaaagtact gacgcatagt
      961 gaacaaaaag aataccaacg cttgaaccaa gcaataaaaa aatatcaaac agagccacac
     1021 aaaaaagctt caggaaatct tcctggagtt ttttctatgg cttcagacga agtgaaagga
     1081 agtgatctga aggagatcaa gcaaaccttc caaagaggac aacaaaaagg ctcaatcatt
     1141 tatcaagatg ttgtctctca tgatcattcg tatcttgaaa aaataggatt gtacgatccc
     1201 aaaacagata ctttagatga agaaggttta aaagcagctg gaagaaaaat gatggagaca
     1261 ttgttcgaga aagagcaact gaatgaaaca ggctactgga tggcaacgat tcaccgaaat
     1321 accaaacata tccatattca ttttgcagtt gttgaaaaaa aaaatacgcg taaaccatac
     1381 actgagattg aaaatggtgt gagttatatc gtaccgaaag ggaagagaag gcaagcaaca
     1441 ttagacgaaa tgaagactaa ttatgtacat gagttagaac gtttttctta tgaaaaaaat
     1501 cgaatgggta tggagaaacg aaatttattg acacggaaaa gtgatttgcg taacacctta
     1561 accaaagtag tgaaagagcc agtacgttat gatcgacacg caatgagctt attaactgaa
     1621 gtatataaaa acttacctaa aaagcgttcg gaatggcatt acggggatga aaatcgaacc
     1681 aaactttcac catacacaag agagaaactc gatgagttaa cccaatatgt gttaacaaaa
     1741 gaacctggat atcaagaata tatacagatt tcaaaagcat tgaaagaaga agcaaaaagt
     1801 ctttatgggg aaagcaagag agaatcaaag gatgcagaaa aaaatgctct ctttgattta
     1861 aaaaagcgca caggaaatgc cattctaaca gaactgaaga aacaggatag tcaaataaaa
     1921 cctttaatag aaaggatgga tcagatgcaa tctatttaca atgaacgatc ctttcgaaca
     1981 cttaaacagt tgccaacgaa tgaatacaaa tatagttacg aagaattcat gaagcgcagg
     2041 aaacaattcc aacaaagagt tgtcacgcaa cggagtttgc gtcaattaaa tcgaacgatt
     2101 aatgcccatg aagaaaaata ccacgcaagt aaagcatacg aagagcaaca acgaagaatt
     2161 caaatggaac aagaaagaca gtaggaggaa tagcaatgaa acaatattta gatcaatgga
     2221 aagttataga aggatcatta agagaagaac gaatcgaaca acttcccgat tgtttagaaa
     2281 aagagcattt gtttcagata agagaaatgt tgataaacga acagtttgat ccaaatcaat
     2341 ttttagtcgt tgagtaccca gctactggtg tgtactgttg taatcatgtg aagggtgaaa
     2401 aatattttat cattcaagaa tatgagggca agttagcgcc ttattatact acttgggaaa
     2461 tgaatgaaga ggggatcaat aacttcccgt gtaaaagcat tgaagagtct atttcactca
     2521 cagaatgtta gttgttaagt gaataaaaga agtaaaagag tcctgaacgt gattgtccag
     2581 gactcttttt tttcactgaa ttgttttccg ccaattcatt gagtggtgcg atttccgtct
     2641 acattatatc aaaagaaagg aggaagaaga aatggcttat tcaacgattg gagttgttgt
     2701 tgaaaaaagc agagacaatc ttgtttttgt gacagaaatt caaacaggca gagcttttgt
     2761 agtcacagac aaagcggcaa aagcatatca aagtggcgat attttaacgt taaatctgac
     2821 tacgaagacg ttcgtcgatg cggcagaag
//
LOCUS       DACESX010000193         2840 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.197, whole genome shotgun sequence.
ACCESSION   DACESX010000193 DACESX010000000
VERSION     DACESX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.197"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            82..1500
                     /locus_tag="IXF88_002620"
     CDS             82..1500
                     /locus_tag="IXF88_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1509315.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            1512..2822
                     /locus_tag="IXF88_002621"
     CDS             1512..2822
                     /locus_tag="IXF88_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1509316.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT          918 a          446 c          599 g          877 t
ORIGIN      
        1 gccaacattg tgaatataaa aacaagaaaa aaagagttat aattttgaaa acgtttacta
       61 gaagaggtag gaggaattca aatgctttca caaaatgctg ttacagcaaa tacaataaaa
      121 aaagaaaaaa aggaatttgg gatttccgat aagataggct acatgttcgg tgatctaggt
      181 aactgtttta ttttaggttt agtcaatagt ttcttgatga tttattatac gaatgttctt
      241 ggcgtttcag gggcaatcgt aggtacgtta ttcttgattt cgagaatttt ggatgcgttt
      301 gctgatgtga tggttggaag attatgtgat gtctcagagt taacagaaac tggccgattt
      361 attccttgga tcagaaaaat gaagtatcca ttttgcttag tgactgtcat tttgtttttg
      421 ccattcgtcc aacatcttcc aatgacggtg aagattgctt atatttttgt ttcatacatt
      481 gtttatggga tttttctctc aacaatcaat atcccgtatg gagcaatggc agcagcaatt
      541 agctcaaatc ctaatgaccg cacctcgttg tccacgttcc gaagcattgg ttctgcgatt
      601 ggcggatcaa ctacaggatt cttcattccg attttaatgt atacccataa caagagtgga
      661 caacaagtcg tttcagggaa tcatttcttt tggatttcaa tcggatgtgc tgcaatagca
      721 tttgtcgcat atatgttaac ttgtaaattg actactgaaa gagtaagagt tgtcaaaaac
      781 gaaaaagtat ctgcttctca gttggtaaaa ggtttgttag ctaatcgagc actgattatt
      841 ttagtcattg ttgacatttt cattgtgatc aatcagattt tagctgggac gaatatgact
      901 tatttattca atgattactt ccataataaa caagcaatgt ctgttgctct tttatttact
      961 tacggaacag tagtagtttt agcaccattt gctacaaagc tgacaaaatt atttggtaaa
     1021 aaggaagcaa gcgtatttgc gttacttgct tcttcatgta tgtacttgct gatgtatttc
     1081 cttcatataa caaatgcatg ggtatatctg attcttttat ttatcgctac tttaggatct
     1141 ggtttattca atttgatggt ctgggcattc attactgacg tgattgatta tcatcaatat
     1201 aagacaggct taagagaaga tggaacagta tatggagtaa attcttttgc gagaaaagta
     1261 ggacaagcat gtgcaggagc ggtaggcgga tttatgctgg cactcattgg ttaccaatcg
     1321 tctacaacag gtggagccat ccaatcagtt ttagtacaag aaaggattta tacgatggca
     1381 aacttgttac cagcattctg cttatttgta tccgcaatca ttttgctagt aggttaccca
     1441 ctgaatcgtt caaaaactgt atttattggt gaagaattga aaaaaatcaa tgaaagatag
     1501 ggagaaacgt attgcggaca aaaaaaggga ttatcaatgc tttgaacttt aaaacaaacg
     1561 acactcaaca agatgataca ataatactgc aaaaagcaat cgatcaaggt gcgaaagaac
     1621 acttacctgt atttattcca aaaggcattt atttagtcgg agcccttttt ttaaaagata
     1681 agagtcatct tatctttgaa gaaggtgcag tcctcaaggg gagaacagat atcgaagcat
     1741 ttccagaaat cgatacacga gtagccggcg tagaaatgaa atggccagca gcgattttga
     1801 atgttctctc ggcgaaagat attcttatag aaggaaaagg aataatcgac ggtcaaggag
     1861 accattggtg ggagctgtat tggggaaaag atcaaaaaag cgggacaaga gctgaatacg
     1921 atcaaaaggg acttcgttgg attgcggatt atgctattaa acgtccacgc gcttgtttac
     1981 tgtatcatgc agaacatgtt gttgttcgtg atttgatttt tcagaaatca ggtttttgga
     2041 acttgcaaat cacttattct aacgatgtat tagtagaaaa agtaatcatc cgcaacaatg
     2101 atggaccaag tacagatggg attgatattg attcttcaac aaatgtgcgt gtctatgaat
     2161 gtgatttggc ctgtggtgat gattgtattg ctattaagtc tggcagagat ggcaatggtg
     2221 caagagtcaa tcgaaaatca tcaagaatcg aggttgctag atgtaaaatc cgttcaggat
     2281 acggtgtaac tattggtagc gaagttagtg ctggagttag tgacgtatat attcacgata
     2341 tcgatttttt tcagtcagac tgtggtattc gaatgaaatc ttcaaaagaa agagggggag
     2401 tgatcgaaaa tatcagggta gaaaatctga atatgattga tgtacaattt cctttttctt
     2461 ggataatgga ctggcataat gaatacaata gaaaaaatag caatgacctt gctcaaatgc
     2521 cagaaagttg gcaagcagtg gctgaagaaa tacctgaatc aaagcaaatg acgaaagtca
     2581 aagacataca catcaaaaac gtacaggcat gcctttctcc aggttactca cttccttccc
     2641 gagcttttga tttagtagca tttcctgaaa agcctataga agatgtctgc ttttctcatt
     2701 gccaaataac tgcaaaagaa ttcgggcgaa tcgaagcagt acaaaactta tgtttcaaaa
     2761 gctgtgattt atctatagaa actggcaaca cagctgcaaa caatactttt gacaatcgtt
     2821 gaaattataa taaggtcaag
//
LOCUS       DACESX010000194         2799 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.198, whole genome shotgun sequence.
ACCESSION   DACESX010000194 DACESX010000000
VERSION     DACESX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.198"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            410..820
                     /locus_tag="IXF88_002622"
     CDS             410..820
                     /locus_tag="IXF88_002622"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1509317.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            813..1268
                     /locus_tag="IXF88_002623"
     CDS             813..1268
                     /locus_tag="IXF88_002623"
                     /inference="COORDINATES: protein motif:HMM:NF015769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1509318.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            1285..2016
                     /locus_tag="IXF88_002624"
     CDS             1285..2016
                     /locus_tag="IXF88_002624"
                     /inference="COORDINATES: protein motif:HMM:NF015566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1509319.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            2033..>2799
                     /locus_tag="IXF88_002625"
     CDS             2033..>2799
                     /locus_tag="IXF88_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1509320.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILA"
BASE COUNT          932 a          446 c          525 g          896 t
ORIGIN      
        1 ttttagatat tttataagaa tacttcatgt cttcgccagt aatataaaga acatctgtct
       61 ctccatttgg acttgggtta tctattttgg gtgtaacata ctatacatac aaccgataca
      121 taaaagattt gacatctgga caaaacacaa tgctttactt catttttata ggctttgttt
      181 acaccatcac aaaaatcacc tcctaattta caaattcatt aaacaaacag aaataaaaaa
      241 tcaccacgat agccgcgttc aaaaaaattt acaaaagaga attttagtta aagtttttag
      301 attcgcccca ctaattacca aaggtatttt atttattctt aatacgaaat aatttattga
      361 catttagatt accaatggta tattatttat aaataaatgg aggtggatta tgtacacaat
      421 tattcttgca agccacaaaa atttagcgaa aggaatgaaa gagacagtcg aatatatcct
      481 tggccctgca ccattcataa aagtaatgac tgcatacaca gacgataact atgatattga
      541 aaaagatacg aatattcttc tcgatggaac tgataaagtc cttgtcatca ctgatttact
      601 tggtggtagc gtaaataatc attggatgaa ttatgtttac gaaaaaaaat taacaaaaaa
      661 aattacagta attgcaggta tgactttgtc cttaattatg gaattaagta tgaacattga
      721 agattataaa ttgagagaaa aaatttccat gattattgct gaaagccaaa agagcattat
      781 aaactgttca gaattaatgg aggtagaaga taatgattaa actattaaga attgacgatc
      841 gcctgatcca cggtcaagtt gcaatggctt ggactaagtc cgtatctgct gactacatta
      901 ttgtagttga tgacgcctct gctaatgaca aaatgaaaca aatgatttta tcattagcaa
      961 aacctgtatc aacgggacta gatattatta cttcagcaga ttttttaagt acctttgaaa
     1021 aaaggaaaaa tcaaaatttg atgatagtca ctagttcccc taaaagcgca tttgaaattg
     1081 taaacgctat cccaggggaa gtcgattcaa ttaatatcgg tggtttacgg tacgccgagg
     1141 gaaaagagaa agttaacgag tatgtggcta tcacatcaga agacaaaaac tacttagcaa
     1201 aattaaagga gtccggcttc aaattaatta tgcaggctac tccaactgga aaagcgaaga
     1261 tttattagaa ggtggggaat aagaatgatt gaatgcattt taattggcat agttgggtat
     1321 ttaacgacaa tagatgaaag atattttggg gcatctatga tgaatcgacc gataatagtt
     1381 ggacctgttg ttggacttat cttaggggat ctccatcaag gcatcttgat aggagcagca
     1441 ttagaagcaa tgtttattgg cattgtaacg attggtgctg cactaccacc agatgtaggt
     1501 gtagctggta ctattgcaac ggctctagca ataaaaagtg gagcaggtgc tgatgtagct
     1561 gttacattgg cgatgccttt cgctgtattg gcacaaggat taaacatgtt ggcttttacg
     1621 attaactcct ttaccgtttc acaaggtaga aaagcaattg aaaaccgcaa attatcatct
     1681 tttcagaagt ggcatttttt tccattaatc gttcgcttac cttccgcagt acttacattt
     1741 ttagtagtat attttggtgc caatgctaca gaaagttttg taaatattat gccagaaaaa
     1801 ttaatgaccg gtttcaccgt agcttctggt ttactccccg ctgttggatt tgccatgcta
     1861 attcaaggaa tgattgacaa aaaatcagtt ccttattttt tcattggata ccttttagcg
     1921 gcatatttag gagtccccgt agttggtgta gctgttgctg caattatggt tgctttatta
     1981 gttatccaaa ctaaacgcaa tcctgtaaat atttaaaggg agagtgataa ttatgggaga
     2041 ttctaaatta actaaaaaag aaataaacaa ggttttctgg cgttcatttg ctttacaggg
     2101 atccgagtca tacactgata tgcaaggaat gggatatagt ttttctatat tacctgcttt
     2161 aaacaaaata tatcaaggga atgatgaaaa atattatgaa gctgtttacc gaaatacgga
     2221 attttttaat acaacgcctc atatttcaac ctttattatg ggtatgtcat tagcacttga
     2281 agaagaaaat gcaaaaacag atcaatttga aactgaaaca atttcatcga taaaagctgc
     2341 attgatgggg cctgtagctg gtattggtga tagtttttac tggggaacat ttcgcgttat
     2401 tgctgctggc atcggcttat ctttagctgc ccaaggaaac attttaggtc caatattgta
     2461 tttttcaatt tatacgctgt tacattttct tggcagatat ggctgcttac gtattggata
     2521 tcggatggga gcaaacgcca ttgaaaaagc atttgagaac aattaccttg aaagtttctc
     2581 gttagcagca tctatcgtag gtttaatgtc aattggagcc atgacctcat ctctggtacg
     2641 ctttactatt cccgccgtat ttaaatcaaa aggtggagaa ataaatgttc agcaaatgct
     2701 cgatgatatt tttccaggaa ttctctcatt ggggctaaca ttctttgttt actttctatt
     2761 aaagaaaaaa gtaaaggttc tttggattat acttgctat
//
LOCUS       DACESX010000195         2581 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.200, whole genome shotgun sequence.
ACCESSION   DACESX010000195 DACESX010000000
VERSION     DACESX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.200"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            77..439
                     /locus_tag="IXF88_002626"
     CDS             77..439
                     /locus_tag="IXF88_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1509321.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            456..1067
                     /locus_tag="IXF88_002627"
     CDS             456..1067
                     /locus_tag="IXF88_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAQ1509322.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(1169..2077)
                     /locus_tag="IXF88_002628"
     CDS             complement(1169..2077)
                     /locus_tag="IXF88_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1509323.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(2119..2433)
                     /locus_tag="IXF88_002629"
     CDS             complement(2119..2433)
                     /locus_tag="IXF88_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1509324.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
BASE COUNT          811 a          434 c          465 g          871 t
ORIGIN      
        1 ttaaggtaga ttgacaaacg agtgatcaaa tagatatact accttcaaac gaccgtttga
       61 atgtgaggtg agatgaatgg aaaaagtttg ccaagtgact atcgtacata aagaaaaagt
      121 aaatgtaata aaaaaaaaat tggatcaaaa agatttttca aacttacttg tgttagggaa
      181 atgtttcagc gattcttcta ggattaaaat tttctatgct ttagaaacct ataaggaaat
      241 gtgtgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
      301 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
      361 ttctttagcg aatgaagaca tcctttccgc tattcgtgtt tttttaaagt tatcagaaaa
      421 tctatctatg aaatcttagt ttggaggaag ttattttgtt acaaagtatt ttttcagcta
      481 tcgctgttta tatttctacc agtattgatt atttgtttat cttgctgatt atcttttctc
      541 aaagccacac aaaaaaaggt attcgtcaca ttttttgggg tcaatatctt ggaacaggta
      601 ttttggtagc cgtaagtttg tttgctgctt atgtgctaaa ttttattccg caagattgga
      661 tcattggtct tctcggtcta attccgattt tcttaggaat cagagtcgct tttgttggcg
      721 aagaggagga agaagaagag gaagtcgttg aaaaactcga atctagagga actaatcgct
      781 tattctggac agttgcctta ataacgattg cttctggagg cgataattta ggtatttata
      841 ttccatactt tacctcgtta gctttttcag aaatcattat tgctttaatc gtattcgcta
      901 tctctgttgc ggttctctgc tatatcagtt acaaattagc caagatttct tttgtttccg
      961 aaacgttaga aaaatacgaa cgaatcattg tccctataat gtttattgcg ttaggaattt
     1021 ttattatgat agagaacggg acgatacaaa cgctactagg tttttaaaaa tcataatcac
     1081 gaaagataaa aagagaggca gcgactaatc aaaatcgctg ccttttttgc ttttcaaata
     1141 atgaagtatt ttttagcgta gaactatctt acgtgttgtg ggagtatccc ttttcaagct
     1201 cttctttgct tgtagttatt tctattgtga tatgattctc tacgcttgat ttgtgtgcga
     1261 cttctgcaat ttcttctttt atttccctag ccttgtctat gtctgaaggg tctataagaa
     1321 ccgtgatgat aagggcgttt tcttccccgt ccagtgacca aatgtgaaaa tgtgagaggt
     1381 cttttacagc aggaacattt ttaatggagt ccgccaagct ctttacgttt acattttcag
     1441 gcgaaccatt caataaaatt ttcattgtgg tgaaaaattc aggaacggtt ttgtagagga
     1501 tataaagtgc aatcaaaatc gataagatag gatccagacg atatgcctcg gtaaagttca
     1561 ttagaatact cacaatcaag actccaatcc accctaatac gtcttccagc atatggaggt
     1621 tcaacatgct ctcgtttttg gatgtgcctt tgcttaaaat ccacgcagca tatccattca
     1681 atgcaatagc tacgagagaa agccagaaca tgccactata atttacgggt tgtgggtcaa
     1741 tcaaacgtgg cacactacga tagatcataa aaaacgagcc actaattagc acaacgccag
     1801 ttatcagcgc gcccagtaaa gaaaagcggt tatagccaaa agtgaaccgt tcatcttgtt
     1861 ttttctctga ataaccttga aaaaaccaag cgaatccaat tgaaatagag tcccctaaat
     1921 catgaactgc gtcggacata atggacacac tattgaaaag aaagccaaag aaaaattcgc
     1981 ttatagagaa aataagattt atgaagaaag caattttaat attctttttc ggttgattct
     2041 gattcgagtg attatggtct ttatctgatt ttgacattta aaaacctcct aagtttgtat
     2101 gctacatact aattggtttt atttttctaa ttcattaaca tgagttaaga cttgttcgag
     2161 aatactaaaa acatgaaggt catctaaact gtaaagcata ctttttcctt ctcttcttga
     2221 tttcactaag cgtgcgtctt tcaacgtttt taattgatga gaaatagcgg actgttccat
     2281 gtccagagaa atagcgatat ttccaacact acactctttt ttctgtaaaa gaaagagaac
     2341 cgacaatcgt gtaggatcac taatcacctt aaatagtttg cttattgaca gaatagatcc
     2401 tttatcgatt ggagaagttg tttttatatc catagtgttt ttattccttt catatgacct
     2461 tccgttcata tctatattac catatgagcg aaacaatgct aatcttttgt ttataaaaga
     2521 gaagtggcgt tctctttatc ttgacaaaat gtatatagcg atataaaatg agtatacaaa
     2581 c
//
LOCUS       DACESX010000196         2571 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.201, whole genome shotgun sequence.
ACCESSION   DACESX010000196 DACESX010000000
VERSION     DACESX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.201"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            426..677
                     /locus_tag="IXF88_002630"
                     /pseudo
     CDS             426..677
                     /locus_tag="IXF88_002630"
                     /inference="COORDINATES: protein motif:HMM:NF014026.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
     gene            735..1037
                     /locus_tag="IXF88_002631"
     CDS             735..1037
                     /locus_tag="IXF88_002631"
                     /inference="COORDINATES: protein motif:HMM:NF025668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509325.1"
                     /translation="MTTTVSNKGSRDGYEVVQLYIGAQYGRYIRPTKDLVAYKKVFVK
                     SGDSVTVSFELTNKDFKYYDEEFNEIIDDGAYWIMVGNDSSQALTNKEEIEVRINE"
     gene            1030..2445
                     /locus_tag="IXF88_002632"
     CDS             1030..2445
                     /locus_tag="IXF88_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1509326.1"
                     /translation="MSNKEEAKSIINFVGGKENIAALTHCVTRLRFALRDDTKVDKAG
                     LENLEDVKGTFNANGQFQVVIGPKVADIYEEVISQSGLSGANKEDVKKVAAELNLNWF
                     QRAVKILGDVFVPILPAIVTAGLLMGLNNLLTNPGIFFEQSLIEVYPNIADVADMINL
                     IANTSFQFLPALITWSTVKYFKGNPLLGIVLGLVFVHPDLMNAWNYGQAVTTGDIPHW
                     TIFGLSISKIGLQAQVLPALVSGYVLAKTELFLKKRVPETIQLLVVAPVAILFTSVIT
                     FIIIAPITIAIGNTIVAGIVWLFQKVPFIGGFAYGIGLAPLVVTGMHHAFLPVHIQLM
                     ANGGDYIWPIGVMSSLAQGSAAIAMNFLIQNKKLKGVGLAAGLSAYMGITEPAMFGVN
                     LKYKFPFVVSILTSGIIGGFMASQHVIATSVGISGIPGFLSIPFEFWPIFFICMLASC
                     VVSFGATYLYGKNKVLLQSFK"
     gene            complement(2475..>2571)
                     /locus_tag="IXF88_002633"
     CDS             complement(2475..>2571)
                     /locus_tag="IXF88_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1509327.1"
                     /translation="ILGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          775 a          409 c          530 g          857 t
ORIGIN      
        1 catttcaggt aatctcaaat aaaaaaatat acagtcctgt ggcactttac ctatgggact
       61 gtatattttt tactctcagc gggcttgttt tgactccgtg tctgactccg tcgtattgat
      121 tttaaatgat attcgatgac catttaacag gtcatttttg aaatttttgt ctctatatga
      181 ctcagtttga taattattga tagatcaatt tgggtctata tcgaacaagg gaataatcca
      241 ataaaaaatg atttagaata tcaaaataac aaaaaagagg actacctgta ttcgtcctct
      301 tttttgacca gattttgacc agtctaattt acattctaat atattcatat gagctaattt
      361 tttgattaaa tggtaagttt ttccttgttt gttttagttt aaactagtgt gtaatttaaa
      421 aatctatatg taagggatta gttgaggctt ggcatctagg agcagaggct ggcaatgcta
      481 ttttggacgt cctttgtggc aaaataaacc cagccggaaa gcttacaata acaattccga
      541 gacatttagg acaaatccct atttattata attatttcaa tactggtaga ccgtattcaa
      601 gtgaaaagtt taatacgacc aaatacttag atgtatccaa tgaaccacaa tttccctttg
      661 ggtatgggtt gagttatacc actttttcta tgtcaccaat tagcattaaa gagaagggta
      721 agaactgttt gaaggtgact actaccgttt caaataaagg aagtagagat ggttatgagg
      781 ttgttcaact atatattgga gctcaatatg gtcgttatat aagaccaaca aaagacttgg
      841 tagcctacaa gaaggttttt gttaagtcag gggatagtgt gactgtttct tttgaactta
      901 caaacaaaga ttttaagtat tatgatgaag agttcaacga aattatagat gatggtgctt
      961 attggatcat ggtaggcaat gattcatctc aagcattaac taataaagaa gaaatagagg
     1021 taagaattaa tgagtaataa agaagaagct aaatcaatca ttaactttgt tggtggaaaa
     1081 gagaatattg cagcactgac tcactgtgtg acacgtctga ggtttgcctt gcgagatgac
     1141 actaaagttg ataaggcagg attggaaaac ctggaagatg tgaagggtac cttcaatgct
     1201 aatggacagt ttcaagttgt tatcggtcct aaagtagcag atatctatga agaggttata
     1261 agccaatctg ggctgagtgg ggcaaacaaa gaagacgtta aaaaagtagc ggctgagttg
     1321 aatttgaatt ggtttcaacg tgcagttaaa atattgggtg atgtatttgt gccgatttta
     1381 ccagcgatcg ttacagctgg tttactgatg ggactgaata atttattaac aaacccagga
     1441 atcttttttg agcagtcact cattgaagta tatcctaata ttgctgatgt cgctgatatg
     1501 ataaatctta tcgcaaacac ttcctttcaa tttctgcctg cgttaataac atggtcaact
     1561 gtaaaatact ttaaaggaaa tcctcttctt gggattgtgt taggtctagt ctttgtacac
     1621 ccagatttga tgaatgcctg gaattacggg caagcagtaa ccacgggaga tattccacat
     1681 tggacaatct ttggattgtc catctctaag ataggactac aagcacaagt attgccagct
     1741 ctagtttccg gctatgtgtt agctaaaaca gagctattct tgaaaaagcg tgtgcctgaa
     1801 acaatccaac tgttggttgt tgcaccagtt gcaattcttt ttactagtgt tataacattt
     1861 attattatag cgccgattac aattgctatt ggtaatacga ttgtcgcagg tattgtatgg
     1921 ttattccaaa aagttccatt tattggcgga tttgcttatg gtattggtct tgcgccatta
     1981 gtcgtgacag ggatgcatca tgcgttttta ccagttcata ttcaattgat ggcgaacggt
     2041 ggagattata tttggcctat tggagtcatg tcaagtttgg cacaaggttc tgctgctatt
     2101 gcaatgaatt tcttaatcca aaataaaaag ttgaaaggtg ttggactggc tgccggtttg
     2161 tctgcttata tgggtatcac tgagccagca atgttcgggg ttaatctaaa gtataaattc
     2221 ccattcgtgg tttccattct gacctctgga ataattggag gatttatggc gtcacaacac
     2281 gtcattgcaa cttctgttgg tatcagtgga attcctggtt tcttgtctat tccgtttgaa
     2341 ttctggccaa tattctttat ttgtatgcta gcaagttgtg tcgtctcatt tggagcaact
     2401 tacctttacg ggaaaaataa ggttctgttg caaagtttta aataaagaat aaaatccctt
     2461 acggtatcta tgatttaagc tgggattccc aataatacct tgatttcagt acagaccgaa
     2521 aacccgaaga gagtgccttc ttttcgggtt ttcttatata atcctagaat g
//
LOCUS       DACESX010000197         2534 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.202, whole genome shotgun sequence.
ACCESSION   DACESX010000197 DACESX010000000
VERSION     DACESX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.202"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(460..963)
                     /locus_tag="IXF88_002634"
     CDS             complement(460..963)
                     /locus_tag="IXF88_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1509328.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1322..2347
                     /locus_tag="IXF88_002635"
     CDS             1322..2347
                     /locus_tag="IXF88_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1509329.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT          814 a          394 c          473 g          853 t
ORIGIN      
        1 gtgtctcaga tttctctact acttctaaaa actactgttt ttttcctttg aaatgccttc
       61 aaaaagtagg atcaggcaga atatactggc agaatcaagg agaagggagc ctaaataagg
      121 agaaaaacca tagaaaatat aaagtaatta cttataaaac aagcttttta tagaaaaata
      181 atatacctga gtagggtata aattaagacg gaattccttt ctagattgag atattgacta
      241 ggaagaatgt taagatttta agcctttaag ctaaaaaaaa accctttttg ttaaataagg
      301 ggaatatagc ctgtcagttt caaactgcaa gtcgtcctgc tgcaataata tagtagatta
      361 gagatgaaaa tagatttttt taaaccaatg actgaataaa aagaccgtca tagatacaca
      421 ttggaatgtg tctattacgg tcttttttta cgaacgattt tattgaagac ggacagttcg
      481 ggtcaaaaat aaaaaagcta cgatcagaaa taagaataaa atgtatccca aaataatggc
      541 tagacggtct aacttatttg tcgatagatg tttgtttaga agttgatagg cccataaacc
      601 tatcagtcct cctgttgtat tagttatcaa atcagtgatg tccgctacgc caataccgaa
      661 taggaattga atcaattctg ccaagaagct tacaaggaaa ataatcccta attttttaga
      721 aaaagaaagt ctcttgaaat tgacattcaa aagcacccca aaaggaaaaa agacaatcaa
      781 gttataagtg gtctcacgta ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac
      841 gagattgaca ttgctgtaag aataagtgag aaattgtgga agattaaatg atagcttgaa
      901 taaaatcaac cataatagaa agaataaata aatccctaat aaaaattttg atacataccg
      961 catagacatc tcctccattt aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa
     1021 agtatgaagt ttaaggacca acgtagttat cgtacgaaaa ttagaagctt ttttttgttg
     1081 ggaatccggt ttctttaaca aagctaagat agcgagttgc ttggaaaagt tgctatcttt
     1141 tttctgtcaa aacgactcgg aatgttcgtt ttttgttcta atttctccaa gttgtttact
     1201 ttttgatact aagtttatga aaaatcaaga aatagttgtt atactaggta atgctgacaa
     1261 acgggattgg catttcatct tggttcagct ttttttccaa ttactaggaa ggagggatag
     1321 attgttcaag acttattttc gtttcaataa aatacaagat aatccttttc gtatcatgaa
     1381 tctgatgcta cttatcttta ttgtattatg gttaggttac ataaatcatg attttttgat
     1441 ttcaagtttt tcatcattag ggatctttac ttttttctat tatcaaaaca taccgatgaa
     1501 atatttgctg aaacgtatgt ttctgatcgg ttgcggactg ttggttgctt tcgttttggg
     1561 gatcttatcg acttatgttt tatggcttga accatttgcc gtagctttgg ttgctttttc
     1621 cagcagattc gtgttgcgat tattccatat ttctaaacca ggtggcttgt tttttgcgat
     1681 gctttctgcc atgggaacga gcatgcagct gccaatcgct caattaccga tcgtctcact
     1741 ttattttttt atgggtgtcg tttttgccct gattgcggca gtgattacaa aattactgga
     1801 ttcacggcct gagcagacaa ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc
     1861 tttagtgatc attgattctg ttttctacag cgctgcctta tttctttccg tttatgtcag
     1921 tcacggcttg aatctgcata atccttattg gttgaccttg tcatgtgcat cgattttgct
     1981 agcagaaaac ttggatgcga tgaagcatcg acaagtccaa tatctgattg gatcgatggg
     2041 tgggttatgt gtgtcggcat tcttgtcatt tgtgccgttt acgcagttgc agaccatttt
     2101 tctgatcact tttctatatg gaatcgcaca atttctagtt gctagaaatt atgcagttgc
     2161 taatattttt cttaatccga tggcgttgat gctatctaca cttattcgtg gagcatactt
     2221 gatttctttg atcgaatatc gttttttagg gatcgtcata ggaagtttca tcggtttagg
     2281 tgttgcgtgg gtaatgacgg ttggtttgca acattattta acggttgttc gtcaaaaatt
     2341 agaatagaag gacaaatata tgtgccaaaa gaggttatga caagattttt gtcacaacct
     2401 ctaattcaag gtgttcaaaa gccagattct cagcattaag tcaaattcac acaaaaatgt
     2461 ggaaaggaac tttcaaaaaa attttatatt tattctatca aaaaattttg tttatgatgg
     2521 taactgaata aact
//
LOCUS       DACESX010000198         2514 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.203, whole genome shotgun sequence.
ACCESSION   DACESX010000198 DACESX010000000
VERSION     DACESX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.203"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(106..1155)
                     /gene="recA"
                     /locus_tag="IXF88_002636"
     CDS             complement(106..1155)
                     /gene="recA"
                     /locus_tag="IXF88_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1509330.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1264..2505)
                     /locus_tag="IXF88_002637"
     CDS             complement(1264..2505)
                     /locus_tag="IXF88_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1509331.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
BASE COUNT          653 a          579 c          452 g          830 t
ORIGIN      
        1 cactcgtata attaaataac tcacgatttc tttattcaat aaaaaaacgg caggtcaagg
       61 agagtaccta ctcttcagac cagccgtttt tgtttatcat aatttttatt cttctactaa
      121 aggcaattct tcttgtcctt tttcatcttc tgggacagcg acttcttcac caataccata
      181 agctgctcta actagagcag aaacttctgc catcatttca ggatgattag ccatatagat
      241 tttagcgttt tcccgtcctt gaccaatccg atcttcttta taagaatacc aagcaccgct
      301 tttatcaaca atgtcttttt ctactgccat gtctagaagt tctccttctt gtgagatccc
      361 taaaccatac atgacatcta cttcagcgat tttgaaagga ggtgctactt tgttttttac
      421 gactttgatc tttgtacggt taccaacaat atctgtacct tgtttcaatt gttctgcacg
      481 acgtacttcc aaacggatcg ttgcgtagaa tttcaatgcg cgtcctccag gagtgatttc
      541 tggattccca aacatcacgc ctactttttc acggatttgg ttgatgaaaa tagcgattgt
      601 ttttgttttg ttgatcgaac cagagagttt acgcaatgct tgagacatca aacgtgcttg
      661 taacccgaca tgtgagtcac ccatttcgcc gtcgatttct gctcgtggaa ctaaagcagc
      721 aactgaatcg actactacga tatctacagc cccacttgat actaaagcat cagcgatctc
      781 tagaccttgt tctcctgtgt caggctgtga aagaagtagt tcatcgatat tcacacctaa
      841 tttttgtgca tattgcggat ctaacgcatg ctcagcatca atgaaagcgg ccgttccgcc
      901 atttttttgt acttctgcaa tagcgtgtag tgcaactgtt gttttacctg aactttcagg
      961 tccatatact tcgatgatac gtccacgggg gtaaccgccg acacctaaag cgacatctaa
     1021 cgcaagagag ccacttggaa ttgtagagat ttgttggtcg attttttcgc ccaatttcat
     1081 aatagagcct ttaccatagc tcttttcaat ttttttcagt gcagcatcta aggctgcttt
     1141 acgatcatct gccaatacga taatcctcct tatatttaca aatcagagca acatttcatt
     1201 atccttatca tatctttttt agtgaaaaaa agcaagaaaa aaccgaacaa atattcgcat
     1261 ttatcatttt cttaaaagct gttttctcaa aagatcaaat ccttgcataa ccgcattata
     1321 tctgatagca gcacgatctc tggcaaagtg atatttttcc gaaaccacgc cgtctttaga
     1381 tgaaatagcg atccatactg ttcctgcaga ttgaccttct aacgtgtccg gtccagccac
     1441 tccggtgaaa gctacactgt aatctgttct tgctatctca cgtgcttgaa tggccatctg
     1501 ctccacacat tctttgctga ctgttccata ttctgctaat agctccggat caatctccag
     1561 aaaaccagct tttgtttgga gcgagtaggt aacgaatccg cctggaaata cttcagatac
     1621 accagcaatg tcgcctaatg cggcttggaa ggcgccggct gtcaaacttt ccgctgctgt
     1681 tacagtctgc ttgttttctt tcagtagttc cactacgact tttgctagcg aattatcatc
     1741 gccgtatcca taaaaatatt cccctactcg ttcttggatt ttttcttcta atgctaaaag
     1801 agcttgattc cctgcctgaa catcatttgt tttcactgtc agtcgtaatg tcacttcatt
     1861 tggtttggca tatggtgcga tcgttggatt gatctgtgtc tcaatcagat cttttagctc
     1921 agtgaccagt cgcgattcac caatcccata aaagcgcaaa actttagaaa ttagtttttc
     1981 ttcagaagga aaacgttcct ctagtaaagg acgtacttgt tccacaaaca ttggtttcaa
     2041 ctcattaggc ggaccgggaa gaaggatata cgcatgtgta tctgtttgat aaaaagtccc
     2101 taaagcaagc cctgtacgat ttggtagagg aaccccgcct tcgataatct gggactgttg
     2161 cagattgttt ttggtcattt ttcgatgggt tgtttcaaaa tatgccaata attttttgta
     2221 tccttccgta ttttgtatga gagacttacc gaggtgagcc gcagttgctt cttttgtcag
     2281 gtcatcttct gtcggtccta atccgccaca taagagtatg agttcacttc ttgtttcagc
     2341 tagagcaatc aactcttcta aacgctgctg attatctcct acaactgttt gataataaac
     2401 ttcgatacct agatcagcta gctgttctga caagaaagtg gcattcgtat tcacgacttg
     2461 tcctagtaaa agttctgttc cgacagctat tatttctgct ttcattactt cccg
//
LOCUS       DACESX010000199         2486 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.204, whole genome shotgun sequence.
ACCESSION   DACESX010000199 DACESX010000000
VERSION     DACESX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.204"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            219..2309
                     /locus_tag="IXF88_002638"
     CDS             219..2309
                     /locus_tag="IXF88_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1509332.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
BASE COUNT          919 a          402 c          474 g          691 t
ORIGIN      
        1 ttaaccgcag tctttttgta ctgcggttta ttattttcta aactatctat tttgccaagc
       61 taccaactat acctaatgag gattttaaac ataccagctg cttccatgcc gctagatatt
      121 atggaaaagc tcattaggaa tccattattt actacgatca atttgatcgt tattgtcgga
      181 atttcactag tcatttataa aaaagagggg atgaagggat gaattttaaa cactcgtgga
      241 caagacggtt aaaatatgtt tttcccataa tgatgatgtt aggtacattg tttgggttaa
      301 aaacagccaa tgtctttgct gaaaaagtta ttgtcgatcc gtcaaatcca atccagaacg
      361 taaagaataa ttttattatt ccaaaagatg ttcctaatgc aaaaacaaat attactgtaa
      421 acggagcgtc aaaagtttac aaatatcatg ttgatgccaa caaaggtggt tttacagacg
      481 actacgtagg attagtggag ggaaacaaaa atatacgcta tccttctttc caaaaagaat
      541 acggtgaaac aaaccttgtt ttagaaggcg taacaaccaa caccaaagtc aatattgact
      601 atggaaaaat cggtacgtat aacgggaaaa aagtcaatat caagctagtt ctatctaata
      661 ttcacttata ctcggatacg ttgccgtgga atctactaga taacaactat acaaaaactc
      721 attttcgaga cgatggctat aaaaatacga atggtgcaat gagtaaatct aaaaaaagaa
      781 cagtactttg gatctcagac aatcttttct caggaattgt ttaccattca acacaaatga
      841 atgtgcagct agtagccaca tatgaggacg gtagccccgt tcaattttca ggagatacat
      901 ttatcagttt taactctttg aaccctgcag gaggaaaaag cacggatctt aaaggagaat
      961 atgcccacta tgacaaaatg aatacgacag attggtatgt cagaaaagat acggttcttt
     1021 cagaatttaa atctttctac aacaatctga acgtggtagg tggccaccca ggaggaagct
     1081 ctaagctgac acaagcggat aatgatttca ataatttgca tgataaatta ggagatccga
     1141 agtttggtca aggtactgtg tcctttaaaa tatctgaagc aaatccgacg tttgtgattg
     1201 gttcttctaa tgttcaaacc tggtttactt tatcttctgc gacaatcttt agtgttgttc
     1261 ctgatcaacc tgaaaaaaca ggggttgata aaaatggaaa taacgtgaac gataaaatgt
     1321 tgcaagtagg agacacgatt caatatcgaa tcaaacagaa ggtgaatcga ttaggagtgg
     1381 atttactagc ggtttacgat cgatttgagt taatcgataa tttaccaaaa gaagtaaatt
     1441 acgtcgatgc acacgttgaa agtggtacaa ataaaaaatt cgatgtatct ggtgaagtga
     1501 cctatgacaa aacaaaacac caggtaaaat atgcagcgaa agctgataca ttaaaaaatc
     1561 gaatgaagta taacggcgaa acttacgaat tagtgatcaa tgtaaaagtc aatgaattgg
     1621 ccaatcaaaa tagtgtagcc aaaaatcagg gaacgtcgat cattaataaa gtagaaaagg
     1681 atacaaatat cattaccgtt tatttcccta aaattccagt gaaagaagta caacaaaatg
     1741 gaaaagatgt aaacggacga aatgacggta agaagggtac gccgacagct cctttaaacg
     1801 caggatctga agtacaatat ctagtcacac aaaaatggca cacaaaaggc gtagacgctg
     1861 cgtcagatca ctacaaacaa tttagtattc aagatccaat tgaagctcgt ttgacatata
     1921 aagaaggttc tgcgcaagta atagataaga gtaaaggaaa agatattact tctgaaggca
     1981 cactaaccta tgatagcaat tcaagaacgc tgaagtggga agcttccgct gacttcttaa
     2041 gtaacaatct tttagatgga cgagaaattc agctgatatt tacagctaaa actccattac
     2101 aatcagaaaa aaatattgat aaccaagccg tagtagcagt agataatgtt tctaataaaa
     2161 caaatgttgt gacaattggg gttgatccta acttgccaca ggtcattgtt cctaaaacag
     2221 gttctacaca tttagtaacg atttcagcgg tcagcttact attacttgtg ttagcaactt
     2281 ttagttatgc ggttctgaga tacatttaaa ataaaaaaac gaaagaatga ataaaatgaa
     2341 aaagttaata atagtagtta cttccttgat tgccatgatt gttattttag tcggatgtag
     2401 taatgaaaaa aataaacaaa ctaatcaaga taatggcgta ttgaaatcag gaactatgtg
     2461 gaaagaagaa gtaggtggtc ttgttt
//
LOCUS       DACESX010000200         2406 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.205, whole genome shotgun sequence.
ACCESSION   DACESX010000200 DACESX010000000
VERSION     DACESX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.205"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..265
                     /locus_tag="IXF88_002639"
     CDS             <1..265
                     /locus_tag="IXF88_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509333.1"
                     /translation="DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMW
                     FEVTVESSDFKEKKKEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            590..1207
                     /locus_tag="IXF88_002640"
     CDS             590..1207
                     /locus_tag="IXF88_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1509334.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1248..1547
                     /locus_tag="IXF88_002641"
     CDS             1248..1547
                     /locus_tag="IXF88_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509335.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1581..1748
                     /locus_tag="IXF88_002642"
     CDS             1581..1748
                     /locus_tag="IXF88_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1509336.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1793..2398)
                     /locus_tag="IXF88_002643"
     CDS             complement(1793..2398)
                     /locus_tag="IXF88_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1509337.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          832 a          380 c          449 g          745 t
ORIGIN      
        1 agattacttt gcgaatactc ctgaagaaca aatacagaga aaacttcctg agtttgaaag
       61 agtaccacct tatgatccaa gagataattc atttcaagta aaaaaattaa aagagtctcc
      121 aaatgaaatg tggtttgaag ttacagttga atcaagcgac ttcaaagaaa aaaagaaaga
      181 atacaattat attgtaaagg caaaagtatc aaaaagattt atttttaacc ctaaattaga
      241 aatatatagt gttgtaatag aataatagtg ttaaaaaaat tgtaaaagtt aaagaagtca
      301 aattctgatg tgaaaaataa aatataccat ggaagacata aaagaaaaac tggataacta
      361 gtttatttaa tggcagaccg ttaaggcctg ccatttttat gtattttagc ggaataaata
      421 taatcatgcg ggagtgtaaa cttactgttt caaaaagtat actttttgac atgccttgat
      481 tttctttgtt tatgtatggc ataataacca cataatacaa tttgaaacgt ttctgattta
      541 atctgattgt tttgaaacaa ttttagtgaa aaaagggtag ataaaaacga tgagaaaatt
      601 tttttatatc cgggtcagta gttatgatca atcgatcgaa cgacaactga tttccgcgaa
      661 ggaattagga atccctgaag aatatatatt tgtagaaaaa gccagcggca aagattttaa
      721 acgccctgaa tatcaattaa tgaaacggat gtttcgaaaa ggagatttac tctatctaca
      781 atcattagat agactaggca gaaataaaca aatgatttta gaagaatggc gagaattaac
      841 taaggaaaag cagattgata tttctgtgtt agacatgcct ctgttagata ctgctagtta
      901 caagcaacta gatgggttag agacgcttat ctctgactta attctgcaat tactcagcta
      961 tatggccgaa gacgagcgga aacgaattaa tagccggcaa agagaaggcg tagaagctgc
     1021 gaaacaaaag ggtgtaaaat tcggcagaaa gaaaattgct ttacctcctg aatttcctgc
     1081 gatctattct gactggaaag caggaaagat aactgcagtc tatgctatgg atcaattggg
     1141 catgaaacga aatacctttt atcgacgggt gaaagaatat gaacatacct tgcaccactt
     1201 ttcttgatag atataacagc tattagacgg gaagggagag tttgaaaatg gacttaacga
     1261 atcaaagggt cgtttttact ggaacgctgc agcaaatgac acgaacccaa gccatcggtt
     1321 tggttcactc tttaaacggc cattgtcaat caagtgttac ttcaaaaaca aatttcctgg
     1381 tttgtggtca gtattctaag tcgttgtttg atgattctct ctatacaaaa aaggaacaaa
     1441 ccgcgcggga cttgattgcg ttaggtcatg agattaccat tttatcagaa gtcgaatttt
     1501 atgcgttaat tagtcaacag ttgaaagaat ggcaacggta tttataatcg ttagtaaaat
     1561 ttacaaggag gaaaattttt atgaagcgaa aatcttatga tcgttggggc attgaaataa
     1621 ctagttttaa tcgaaatatt gcttttcacg tggagtgtga ctgtggggaa ttagcaaaaa
     1681 gtgtttatca aaaaaattac ttttactgtc ctaaatgcca tcgccgatac ggggtacatg
     1741 gaacatagaa acgaaatagt gcggtagaaa agcgaatgaa tgtagtatat ttctacaaat
     1801 cattcgcttt ctttatttta taaatagttg gtcgagagat cccgttttct tttgcaatct
     1861 ctgcaactgg aatgccttgc tttaacatgt ccaccacggt tttaaaaact aagcgttttt
     1921 gcggatctgt tgagttaata gaatactcga ttggtcgtcc cttatatcgg ccggcttttt
     1981 gagccaataa tactccttgc cgctgacgtt cacgaatttt ctcacgctcg ttttgtgcaa
     2041 tataacttag cagtgataaa aacatatcga acatagcggt atccaggagt tcatttccag
     2101 tattaaaatc taaaaactgt gcatctaata tttttaacgc tacttttttc tgtttcataa
     2161 aagcaactgt attttttata tcttcataat ctcttcctaa acgatctagt gatgtaacga
     2221 tgacgcaatc accttcacgg atataattta gtaatttttg aaactcagga cgctcagtat
     2281 ctttaccact ggctttttct tggaataatt tatcaatgtt aagtccttcc atcattgcca
     2341 attgtcgatc caaattttga tcttgtgaag atactcgaat atagccaatt tttcccataa
     2401 aaaact
//
LOCUS       DACESX010000201         2325 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.206, whole genome shotgun sequence.
ACCESSION   DACESX010000201 DACESX010000000
VERSION     DACESX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.206"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..544
                     /locus_tag="IXF88_002644"
     CDS             <1..544
                     /locus_tag="IXF88_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1509338.1"
                     /translation="TPMILLIIGIIFTTVGFSLSSSNEANDNNKQESSSSDTQTTTSN
                     SSNKSLTQFEEFLETNNHDWGAFLDSYYSITPATEQNTAFTKYISGKTYTFEGTVIES
                     MTTRIAIIADKEYDDKSWNDISATPKVSYVIFAKDLNNADTFTKGDKVTFTGEISSRG
                     SNIEKSYAQWDMINSKVSKK"
     gene            657..1292
                     /locus_tag="IXF88_002645"
     CDS             657..1292
                     /locus_tag="IXF88_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509339.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            1591..>2325
                     /locus_tag="IXF88_002646"
     CDS             1591..>2325
                     /locus_tag="IXF88_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1509340.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETS"
BASE COUNT          915 a          362 c          399 g          649 t
ORIGIN      
        1 aacacctatg attctcctta ttatcgggat tatttttact accgtaggtt tctcgctttc
       61 ctcatcaaac gaggcaaatg acaataacaa acaggaaagc tcatcatctg atactcaaac
      121 tactacatct aacagcagca ataaaagcct aactcaattt gaagaatttt tagagacaaa
      181 taatcatgac tggggtgctt tcttagatag ttattattct attactccag ctactgagca
      241 aaatacagct tttactaaat atattagtgg taaaacatat acttttgaag gaactgttat
      301 cgaatcaatg actactagaa tagccattat agcagacaaa gagtacgatg ataaaagttg
      361 gaatgatatt tctgcaacac ctaaagtttc atatgtaatt tttgcgaagg acttaaacaa
      421 tgctgatacc tttacaaagg gcgataaagt aacttttaca ggagaaatta gttcaagagg
      481 atcgaacata gaaaaatcct atgctcaatg ggatatgatt aatagtaaag taagtaaaaa
      541 ataaatgaac cacgccccac taagagttag attgtgcaca ataataaaac ctatacaaaa
      601 gagtgccatc aggagtcaca atgcttgtaa aggaaatgac aaaataggag gtaactatgg
      661 ataatagcaa tgaaaaacta accaaaaaag aatatcttga attaatagtt gaaggcggta
      721 ttggagcaat tccttatatt ggtggcgttg tgcagtcact ttattttggg tctaaaaacg
      781 agaagcgttt caaacgcatc gagaaattct acaaaaattt aaacgaatca ttagaaaaca
      841 ttcgcgatca aattccagaa gatgttttca acttagagaa caaggaccaa ctcattggaa
      901 tattcgaagg gataaatgac gaggtagaaa aatcaaaggc acaaaataaa atagatatgt
      961 ataaaaatct ttataagaat tgtttacttc gaattaacaa cgcttcatgg gataacgagg
     1021 agtattatct tcttgtactg aatcaattaa catcgattga gattcaattg ttagcatgct
     1081 taatgagtcg tggaaacgaa aatttcactg gaaatatcag catgtctggc tattcacaag
     1141 agctaataga tggtagctta aatagattat ctgattttgg tctgttagaa aaaacgatta
     1201 gctccatagt attaggtgat gtcggcaaac aaaatatggg ctacaaaata tctcatttag
     1261 gaattcaatt tattaactca actcttactt aagttttctt tctaatatga catctagacc
     1321 tatcagcaat gcaacaataa aaaaccagac ataccaataa tcaacaaata atctaaactc
     1381 tggaagttca gttattcttt taaaaatcac tttaatcata ataaacacct ctgattggat
     1441 gatctaatac acatccaatt ataccattaa aacgacaaat agcacatcct tgcgccggca
     1501 agcaaaaagg atgtgcaaaa aataataaaa cccaaacaat gggcttcttt atagttccta
     1561 ttgtatcaga gaaagagagc tgattcaatt atggcaagat tagtcaaacg tggaaatagt
     1621 tggcaatacg aaatttcata caaaaaagat gacggaaaat acacgaagat aagaaaatca
     1681 ggatttaaga caaaaggaga agcaaaagat gccgccaacg aattagaata taacttgaac
     1741 aaaggcctta aaggggatcg caaaaatcta ttattatcag attactttga ggattggatg
     1801 caactttata aagaaggaac agtatctcct atcacttata gaaaatacga agatacgtta
     1861 atgaacataa agaaatatat gccagcggta ttgatttctg atttagatag agtgggatat
     1921 caacgctttt taaataagta tgctaaagac catgtaaaat ctaccgttat taagtttaat
     1981 aaccatatta gagcgtcgtt gaaagatgcc gtagaagaag gattaattcc gtttgatcca
     2041 actagaaaag cagtaatcaa aggaaaagat tcattgaagc caaaagaaga taaatattta
     2101 gattatgatc aatttaaatc tttaatgaaa ctcgtagaag aaaacctttc tgcgcagtac
     2161 tcttctccta tgctcgtgtt agttgctggt gccactggaa tgcgatttgc tgaacttcta
     2221 ggattaacat gggaagatat cgatttcgaa gatcaaatca tcactattaa taaaacatgg
     2281 aattataaat taaatgaatg gggaaaaaca aaaaacgaaa cttca
//
LOCUS       DACESX010000202         2293 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.207, whole genome shotgun sequence.
ACCESSION   DACESX010000202 DACESX010000000
VERSION     DACESX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.207"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            98..1960
                     /locus_tag="IXF88_002647"
     CDS             98..1960
                     /locus_tag="IXF88_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1509341.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELPNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1960..2292
                     /locus_tag="IXF88_002648"
     CDS             1960..2292
                     /locus_tag="IXF88_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1509342.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          824 a          342 c          452 g          675 t
ORIGIN      
        1 gttaaaaagc tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta
       61 gaaaacgctt atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga
      121 aaggcagctt ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc
      181 tgaaaaagaa aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg
      241 gctgaatgag cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat
      301 tagtagctct gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat
      361 atttgcagat caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga
      421 agctattact ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa
      481 tgatttagat gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc
      541 gcgaaagggt ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat
      601 tcttcaaaaa gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg
      661 cggtgagact gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt
      721 taatcaagta atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa
      781 ttacgcggaa ttaccaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa
      841 agaggctacg attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct
      901 ttcttatcgc gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga
      961 atatcattat atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact
     1021 agttgagaat gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc
     1081 acaattgttg acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt
     1141 tgtcaatgaa tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga
     1201 tgcgatcaag caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc
     1261 tttcgtggca tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg
     1321 aagcgttaga gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca
     1381 aaagatcaat gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc
     1441 gcaagaaatg attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg
     1501 catcgatcgt cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat
     1561 tcaacaaata ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa
     1621 tcttagagaa acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt
     1681 gaaagcttat acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga
     1741 atatcgagaa gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca
     1801 acaatatatg gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt
     1861 aggaaaaatc gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag
     1921 tgcattattc tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct
     1981 ttaaaaatta tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt
     2041 gaagagcgat tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt
     2101 aatcacttga atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat
     2161 cgggatttgt tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga
     2221 agtatcggta atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca
     2281 aaaaataatt aat
//
LOCUS       DACESX010000203         2293 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.208, whole genome shotgun sequence.
ACCESSION   DACESX010000203 DACESX010000000
VERSION     DACESX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.208"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(51..1598)
                     /locus_tag="IXF88_002649"
     CDS             complement(51..1598)
                     /locus_tag="IXF88_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1509343.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IXF88_002650"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IXF88_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1509344.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IXF88_002651"
     CDS             complement(2043..2237)
                     /locus_tag="IXF88_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509345.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DACESX010000204         2257 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.209, whole genome shotgun sequence.
ACCESSION   DACESX010000204 DACESX010000000
VERSION     DACESX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.209"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            91..1704
                     /locus_tag="IXF88_002652"
     CDS             91..1704
                     /locus_tag="IXF88_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1509346.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          709 a          401 c          444 g          703 t
ORIGIN      
        1 tattcaaaat aaacaataat tttccggcac aattatttgc tgttttttac cataacatag
       61 tacgatcaac gtagaaaagg agatgttttt atgaaagttg ttgtcattgg cggtgtagct
      121 ggcggtcctt catttgccac tcgtttccgt cgattgaatg aagcgcacga aatcattatc
      181 tatgaacgcg gagaaaatat ttcttatgca agttgtgctt tgccttatta tttaggaggt
      241 gtgatcactg accgtgactc gctgatcgaa cgtacaccag aaatattgaa aacaaaaaac
      301 aacatcgatg tctttactaa acacgaagta acagcaatcg atccttctac taagcgatta
      361 acagttaaag acctatccac aaatgaagaa ataaaaacag attacgataa gttgatcatc
      421 tcttctggtg ctagaccaga ttatccggat attcccggag tttttgaagc agaaaacggc
      481 tttgtcctcc gtagtgtgac ggatgcggat cgaatcaaat cgttccttga agaaaaaaat
      541 ccacaacatg tcgtcattct tggtgcaggt gttatgggtc tggaattagc tgagaatttc
      601 aagcatcgcg gcttagacgt gactttaatc gatcaattgc cacaagtcgc tttcccttat
      661 gatccagaaa ttgctaattt agtttatgac aaattgctga aagaaggatt agccgttcat
      721 ttagaaacaa gagttactga gatccgtgat aaaggtcgag aaatagtatt atcagatggc
      781 tccgtccttt ctgctgatat gctaattttt gctgttggtg tttctccgaa gaatgaagtg
      841 gtgaaagcaa ccggcataca attatcagat acaggtcaga tcattgttga tgaccagtta
      901 caaaccaatc tgccggacat ctatgcgatt ggcgatatta tcgaaacgac tagtgtagtg
      961 actggtcagc cgatccaaag tatgctttcc agtgcggcca atcgtcaagg acatatgctg
     1021 gcagatattt taaatggtac gcctatgcgc tatcgcggat atattggtgc aggtgtcgca
     1081 aaaatctttg atcatacagc aagttatgcg ggaatgacag aacatgaatt aaaagcatca
     1141 ggcataacaa attataaaac tgtttttatc actccctttg accatgccta tttctatcca
     1201 gaagctacaa gattaaatct aaagctgatt tttgatgcag atagcggtcg tattttaggt
     1261 ggacaagcat ttggagaaaa aggtgtcgat aaacggatgg gagaactttc tgtagcaatc
     1321 accggaaact tgacagtctt tgatttgcct gatttggagt tgccttactc tccaccgtat
     1381 tctactaccc gtgacccgtt gaatatagct ggttatgtcg caatcaatca aatgacgaat
     1441 atcgtagaaa cgatcaaagc aagtgatata cccgaaaacg atttgaaaga agcgttcttt
     1501 ttagatatac gtgaacctaa taaagcacct tctggcagta tctcggcaac aaaaaatatt
     1561 ccaatgaatg agttacgaga tcgaatcaat gaaatcccaa aagataaaaa agtttacatt
     1621 actttcagaa gaggattgaa tacttatact tctgcccgaa tcttggcagg tttgggtatc
     1681 aaagcgattt tgattgaaga ataaaaaaac gaccgcggtg ggtagccgcg gtctaggagt
     1741 tatttttact tgaggagtaa aaatgaaaaa gtatataggg ttgttggttg gtatgtatat
     1801 agaatagaag ataaatgtga agaaattgtg cattatttct tcacatttat taggttatag
     1861 tttaagaaag tttaaacatt gcgcaattta attattttag ctgtttcttt tcataaaaaa
     1921 acatgttgtg actctgagac aaatcatagt ccatagtgat gtctctagag taactacgcc
     1981 taatattacg aggcatcata ttaatctatc agcactagta cctaaactag catccctttc
     2041 gaatattttt tcttatataa taaagtttgc tttcactcct ctaatctcac caaatcgact
     2101 ggcttgcgtt gtgtgatact aggaactgat tggtttagaa cgacttttct attgtaatca
     2161 tatttttcgc catcctcgtt gtcttctaat ccttcaatga atgatcccta atgtgtgtat
     2221 taaataattt aatttttttg ttcctattta tttaaca
//
LOCUS       DACESX010000205         2251 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.210, whole genome shotgun sequence.
ACCESSION   DACESX010000205 DACESX010000000
VERSION     DACESX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.210"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(146..2143)
                     /locus_tag="IXF88_002653"
     CDS             complement(146..2143)
                     /locus_tag="IXF88_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1509347.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTTDEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          572 a          503 c          411 g          765 t
ORIGIN      
        1 ttgtcacaac cttttttgcc gagtaaacgg tgttcaaaag ccagtttctc ggtatgaagt
       61 caaatttaag caaaaatgtg agaagctatt ttcacaaaat ttttcttcat actcgaaact
      121 aagcggcttt ttcacgacct cttttttatt ttgccatgaa catatcataa tacgcgtcca
      181 ttatttcttt agcaatcgtc aagtttatgt ggtcttgttc gtctaataag ttcggcaaga
      241 caacactgat ggcaatttcc ggattgtctg ttggtccgta tgccacgata ttactattga
      301 ctgtcgcaat atctggatga ccttccgcaa ccgtttctgc agtccctgtt tttgcggaaa
      361 ggtccatttt cgcactggct aatggtttgg ctgttgtgta aggatcggta ccatggacaa
      421 cttggtggaa tccttcacgg aggatcgcca tattttctgc agaaatattg accgtatttt
      481 ctactgttgt gccaatttct ttcttcactt cacccaagtt tccattttcg tcattgccat
      541 agatcgcttt gactaggtgc ggctgtactc gtttcccgcc attagcgact gttgaagcat
      601 attgagctaa ttggattggt gtatatgtgt caaactgtcc aaatgccaaa tcggtaaagt
      661 tacctggagc ataccattct ttgccattat ccgcattgaa cttcttcata taatcaacag
      721 aacgtgagat cccaggagat tcattcggca aatcgatccc agtcgttgta ccaagaccaa
      781 actctttgaa agagtctctc aattgctgat aagctttagc ttcttcatct aaactaggta
      841 gtcccattcc aggtgtatag tctagtccta acatctttaa cgccactttg atcatatacg
      901 tattagaaga caattccgat gctttgactg catcaagagc aacttgtccg cttcgattaa
      961 agacagagct tttttctgaa gcaccttgca gacgaatcgg ctcatcaatc aaaacttgat
     1021 ttccgctgat cgcgttactt gcccagcctg ctgtcaaagt accagccttg actacagacc
     1081 ctggatcaaa ggcgctagta atcgtgccta aagcattctc ggtgatttct ttggaacctt
     1141 gttcatgact gaaaccagtc attgctagca cttctcctgt ctgaggattc atagccactg
     1201 cataagcacc aggcgaatac tgtgctttgc cgttgttgat caatgtttga taattccttt
     1261 tcaaaatttc ttctactttt gattggaatt ctgcattcat agataacatt aaattagaac
     1321 ctttttcccc agagaagatt tctttttgat tcgaaacatt tccttcatta tcaagggaaa
     1381 cttcatattg ggttttggtt ccttgcagga catcttcata ttgtttttcc aaataacttt
     1441 gtcctacacg gtcattccga gaataacctt ttgctaggta tttctctgct tcatctgctg
     1501 gaagtccttg cttttcagtc gtcacactgc caagaatact tttcagtgaa cttgctgcat
     1561 tatattctcg tgtccagtct gtgccagtcg aaactcctgg aagttcagaa gcgcgttctg
     1621 ccacaacagc taactcttga tcagtaacac cagagttttt gatatagaca gtgtttaatt
     1681 catatgcact attcatccgt ttaaagatgg tagcaatttt taattgctcc tcgtttaaat
     1741 taatctcatc agttgtgact ttattgacca tcgcactata ttgttcgctg ttccctaatt
     1801 gcttttcttt tgccgaaagt ctttttgtgg cttctttcaa atgtttggga tcagccagcc
     1861 aaaaatcttt tttatctcta tctgtcagat tttcatctac cggaacatcg atcagcgagt
     1921 tcagcttcgt tgcgatttca agcaaatctt tagccgtaac ttgtgttcct ctcgtatatg
     1981 tgatggcctg attcgcttta ttcgtaacta aagcttttcc tgtggagtcg tagatcattc
     2041 cgcgaggagt actttcttgg accgtaatcg tagaagtaga cttcactcgc gcctccatct
     2101 cttcgccgtt cacgatctgc aaataaccca atctagcaat caacgcaaca aataacgtaa
     2161 atataatgaa aaaaagaaaa tttaagcgaa aaggaacatg ggaccggcga aacgttttgt
     2221 tcttaggttc ttgtgcacct tttcttattt t
//
LOCUS       DACESX010000206         2246 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.211, whole genome shotgun sequence.
ACCESSION   DACESX010000206 DACESX010000000
VERSION     DACESX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.211"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            14..1453
                     /locus_tag="IXF88_002654"
     CDS             14..1453
                     /locus_tag="IXF88_002654"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1509348.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            1455..>2246
                     /locus_tag="IXF88_002655"
     CDS             1455..>2246
                     /locus_tag="IXF88_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1509349.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGY"
BASE COUNT          794 a          361 c          407 g          684 t
ORIGIN      
        1 gaaggagtaa aatatgaact tacctcgaga aatacgatat cgtgcttatt ctgattggac
       61 taaagatgag atggataaaa ttaaggataa tgtaaaacga tcaccttggc gtgctagcta
      121 tcatatcgaa cctaaaacag gattgttaaa cgatcctaat ggtttttctt tttttaacgg
      181 aaaatatacg cttttttatc aaaattggcc atttggagct gctcatgggt tgaaagaatg
      241 ggtacacaat gaatcagatg atttagttca ttttcatgta acaggagcag aacttcttcc
      301 agacacaaaa cgagatagcc acggcgctta ttccggttct gcatatgaaa tagaggggaa
      361 tctattctta ctctatacag gaaacgttcg aaatgaaaat tgggaacgtg atacacttca
      421 aatcggagca tggatggata aagactacaa cattacgaaa ggtgagaatg ttcttattca
      481 taaacctgct gatgtaactg atcattttcg tgatccacaa atgtttaatt atgaaggaca
      541 attgtatgcg attgttggag cacaaaattt agaaaaatca ggctttatca aactttacaa
      601 agcggatgac aataacgtgg aaacatggga ggaagttggc aaccttgatt ttggcggcac
      661 tggttcagaa tacatgattg agtgtccaaa tctagtattt gtagacaaaa aacctgttct
      721 catttattgt cctcaagggt tggataaatc tgagctaaat tatgggaata tctatccgaa
      781 tacttttaag atctgtcaat cgcttgacat agaaaaagta aaacttgtgg gcgcttcaga
      841 aattcaaaat cttgattatg gatttgaagc gtatgcgact caaggattca atactcctga
      901 tggtcgtgct ctgattgtca gttggattgg actacctgat atcgattatc caactgataa
      961 gtatgattat cagggagcaa tgagtcttgt aaaagaactt tcaataaaag atggtaaact
     1021 ttaccaatat ccagtagaag ctattacatc attacgtgct agttctgaac aatttacagc
     1081 aaaagcagaa acaaataata cttatgagct tgaactaact tttcctccaa atcagaaatc
     1141 ggagttactt cttttctctg atagtaaagg aaatggactt agcttgattg tagataccaa
     1201 agaaggaaaa attattttag atcgcagcaa agcaggcatt caatatgcca ctgactttgg
     1261 tacgattcgt gaatgctcaa ttgatactaa agaaacaact gctaacattt ttgtagataa
     1321 ctcaatcatt gagattttta ttaataaagg agaaaaagtg tttactagtc gtgtattccc
     1381 tgaaaatcac caaaatggta ttcaaatcaa atcaggcgaa cctactggaa aatactttga
     1441 attaaaatat taaaatggta gcaaaattaa ctgatgtcgc tgaattagct ggagttagcc
     1501 caacaaccgt ttcacgcgtc attaataata agggatattt atcagaaaaa acaaaacaaa
     1561 aagttcatga agctatgaaa actcttggtt ataaaccaaa taatttagca cgtggacttc
     1621 aaggaaagtc acctcagctt attggtctta tttttcctaa tattagtaat atcttttact
     1681 ctgaactcat tgagtatctc gaaatagagt tatttaaaca tggatataaa acgattattt
     1741 gcaatagtca aaatgatcct gctaaagaga gagaatacat tgaaatgctt gaagccaacc
     1801 aagttgatgg cataatctca tcaagccata atcttggaat tgctgattat gagcgtgtga
     1861 gagcacctat tgttgctttt gatagaaatt tagctcctaa tgtttccatt gtttcttcag
     1921 ataactttga agggggaaaa cttgcagcta agactttaca aaaaaatggt tgtcaaaata
     1981 ccattatgat tacaggaaat gataacacgg attctcccac agaattacgt gcactaggtt
     2041 tttcttttca aaatccaaat ggaaaaattt tcaaaattcc taataatctg tctactatcc
     2101 gtcgtgaaat ggaaataaag tcaactatta taaatcacaa accagatggt atttttgttt
     2161 ctgatgattt aacagccatt ctcactatga aaattgctca tcaacttaaa cttaatatcc
     2221 cagaagattt aaaaattatt ggatac
//
LOCUS       DACESX010000207         2235 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.212, whole genome shotgun sequence.
ACCESSION   DACESX010000207 DACESX010000000
VERSION     DACESX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.212"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            106..>2235
                     /gene="acm"
                     /locus_tag="IXF88_002656"
     CDS             106..>2235
                     /gene="acm"
                     /locus_tag="IXF88_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1509350.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTIC"
BASE COUNT          876 a          345 c          443 g          571 t
ORIGIN      
        1 tttagttaaa agattggaaa tgagtggggg aggaattgaa gaaaggattt ttgagagatg
       61 atatagtagg ttttattttt agttaaaata ggggagatag aaaaaatgaa aaagtgtaga
      121 aatattattt tatcaatgtt atttattatt actaatataa catcactgat tcctgttcat
      181 gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac cgtggaccca
      241 acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa
      301 aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa tattcgcggt
      361 gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg aactttaacg
      421 gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct agactcagta
      481 gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat
      541 acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca
      601 gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct acctgaagat
      661 actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt tgaacaacca
      721 gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac gtttgaaatt
      781 aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat ccaacaattt
      841 ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta cattgaaata
      901 actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa gacgatcata
      961 gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa agaatttaat
     1021 aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc agcttctggt
     1081 ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa tgacacagag
     1141 attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc tattcaagga
     1201 cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa
     1261 gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt tgatccacta
     1321 agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt cagtttacct
     1381 atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa cggtggaacc
     1441 actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca
     1501 gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac ttgggaagat
     1561 atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga agtagatcaa
     1621 aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct agtcgttaca
     1681 aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga caatgagaac
     1741 caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg tcaaccgatc
     1801 caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa
     1861 tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc cgaatacagt
     1921 acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac aaatatatct
     1981 ataccaggta gccatataac accgtggaaa aattttccag agaagacaga gagagagaaa
     2041 attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga atccagcgaa
     2101 caaagaagca attatattag taagagagct gtacctaaac agataacaaa taaatcaaaa
     2161 agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat tggtttattg
     2221 atagtaacta tctgt
//
LOCUS       DACESX010000208         2202 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.213, whole genome shotgun sequence.
ACCESSION   DACESX010000208 DACESX010000000
VERSION     DACESX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.213"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..417
                     /locus_tag="IXF88_002657"
     CDS             <1..417
                     /locus_tag="IXF88_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1509351.1"
                     /translation="KDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFC
                     QLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTE
                     GLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            472..2037
                     /gene="guaA"
                     /locus_tag="IXF88_002658"
     CDS             472..2037
                     /gene="guaA"
                     /locus_tag="IXF88_002658"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1509352.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT          683 a          459 c          458 g          602 t
ORIGIN      
        1 aaagattcca cgacatggga gccactcaaa cggcattatc atcgactgtt taaacgacca
       61 attagtcaaa cagaaatcgt tgatgagctg ttaagctaca acgaagaatt gcgaactgcg
      121 tatcattttt gtcaattatt acgctattat ttcgttaaac gaggtacgga aggctttcaa
      181 gaaaccctta aaacaatttc atctacgtta cctcagtcgt ttaaaaagaa attcacgatc
      241 ttccattgct accaatcggg tatttcaaat gcctttaagg tgtcccactc aaacggcgtc
      301 actgaggggc tgaataacaa gatcaagctg attaaacgaa ttgcgtttgg ttatcgcaac
      361 ttttataact ttcgggcacg gatctattta caacaaggtc tgatctttga aaactaaaaa
      421 aatatgcggc gcacagaacg cgccgcatat cggactaagt tttattcaaa tttgtcttca
      481 ccaacacgat ttgacgaaga gccaaaaatt atcgtacttg attacggtag tcagtataac
      541 caactgatca ctcgccgtat tcgtgagttc ggtgttttct cagagttgat gagccaccgc
      601 attaccgcag aagaagtaaa ggcaatgaac cctaaaggaa tcatcctttc tggcggacca
      661 aacagtgttt atgatgagaa ggcctttggt attgatgaaa agatttttga tttggggatc
      721 ccagttttag gaatctgtta cggtatgcag ttgattactt ataaattagg cggaaaagtt
      781 aaaccagcca aaaaccgtga atacggaaaa gcagaattgg aaatcacttc tgcagaagcg
      841 gcccttttcc aaaacactcc tgaaaaacaa accgtttgga tgagtcatgg tgacctagtt
      901 acccaagcac cgactgattt cgatgtcgtt gctaccagca aagattgccc catcgcatcg
      961 atccaaaatg cagaacgcaa aatgtacgga atccaattcc atgcagaagt tcgccattcc
     1021 gaatatggaa atgacttgtt gcgccatttt acactagatg tctgccaatg tactggtgac
     1081 tggacaatgg ataacttcat cgacatggag atcgcaaaaa ttcgtgaaca agtcggtgac
     1141 aaaaaagttt tattaggcct ttcaggcgga gtagattcct ctgttgttgg tgttctttta
     1201 caaaaagcga tcggcgatca attaacatgt atcttcgttg atcacgtatt gttgcgtaaa
     1261 ggtgaagcag aacaagttat ggaaagtctt ggcggaaaat ttggcttgaa cattataaaa
     1321 gtcgatgcca aagaacgttt cttatcaaaa cttgctggcg tgtctgatcc agaagaaaaa
     1381 cgtaaaatca ttggaaatga atttgtatat gtcttcgatg atgaagcaac caaacttgcc
     1441 ggtgaagaag gcgtttcttt ccttgcacaa ggaacccttt ataccgatgt gatcgaatca
     1501 ggaactgaaa ctgcccaaac catcaaatca catcacaatg taggtggatt acctgaagac
     1561 atgcaattca agctgatcga gccattgaac acattgttca aagacgaagt acgtgaatta
     1621 ggtacccaat taggcatgcc tgacagcatc gtttggcgcc aaccattccc tggaccaggc
     1681 ttaggtattc gtgtccttgg tgaattgaca gaagaaaaat tagaaatcgt ccgtgaatca
     1741 gacgctatct tgcgcgaaga aatcgccaat gccggtttag atcgcgacat ctggcaatac
     1801 ttcaccgtcc taccaggcat tcgctctgtt ggggttatgg gagatggccg tacgtatgac
     1861 tacactgtag gtatccgtgc tgtcacttct attgacggca tgaccgctga ctttgcccgt
     1921 atcccttggg aagtcttgca aaaaatcagt gtccgtatcg taaatgaagt cgcacacgtt
     1981 aaccgtatcg tgtatgatat taccagcaaa ccacctgcaa cagtggagtg ggaataaaga
     2041 gccaacataa tatggatgag gaatacctat aaatcaacat tattcaatta ctaaagttcc
     2101 tgattttcgt aaaaaataag atggtttcct accattttcc ctaccaaagc ccttgttaga
     2161 ttaactacta acaagggctt tgcttttatt cactaccttt at
//
LOCUS       DACESX010000209         2197 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.214, whole genome shotgun sequence.
ACCESSION   DACESX010000209 DACESX010000000
VERSION     DACESX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.214"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(119..709)
                     /locus_tag="IXF88_002659"
     CDS             complement(119..709)
                     /locus_tag="IXF88_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1509353.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1232..1393
                     /locus_tag="IXF88_002660"
     CDS             1232..1393
                     /locus_tag="IXF88_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509354.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1412..1906
                     /locus_tag="IXF88_002661"
     CDS             1412..1906
                     /locus_tag="IXF88_002661"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAQ1509355.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
BASE COUNT          788 a          338 c          372 g          699 t
ORIGIN      
        1 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
       61 ccatcagtaa aattacttgt catactattg ataaggagta tttaattgaa gagaatgatt
      121 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
      181 tctttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
      241 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
      301 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
      361 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
      421 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
      481 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
      541 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
      601 tagaagcagt tttgttttgc catccctctt tttggtttag tgtgctagta atataagcac
      661 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
      721 cctttactat ctatgacaag gacaacagtt ttaacataca gacattcatt aaatttggat
      781 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagaca gtcaacttaa
      841 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
      901 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tcttaaaaga
      961 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
     1021 tgaaaacaac cctagaaata tataactagt ccaatgaaaa aaaagcgaat gccgtagaaa
     1081 acaaggattc tgcgtacatt catatctcat tttgcgagca tatctgctat tactgtgact
     1141 aaatgttaat atggttaaaa tgaccgttat attttttata taaatatcgt ataattagta
     1201 tgaatataga agaaggaaag aaggtgcaat cttggaacct aaagacaagt atttagatta
     1261 cgctagggtc aataaagata aatttattga aacaatcatc aagggaaaaa tccctgatga
     1321 tgaaaaagat gcggtattca tggctggaag cccaggagca ggtaaaacag aggttgcact
     1381 aggtttagct taaaattatg acaaccatgt tgtgattgat gcggactatt ttagagcaca
     1441 atttccagaa tacaatggaa agaactcaag tgtttttcag aaagcttctt cttggttagt
     1501 tgaacaatca ttaaaatatg tactggaaca tggctattct ttcatactag aggatacttt
     1561 tgctatcctt agtgcagaga aaaatattat tcgagccctt aaaaataact ttcgcgttac
     1621 gattttctat gtttaccaag atcccaaagt tgcctgggat ttcactcgaa aacgtgaact
     1681 agctgaagga cgtcatgtgc caaaagaaat gtttatcaat gcgtttttta agtccagaga
     1741 gaatattgaa aaagtcaaaa atagacatcc tgaagtagtc cttcacataa tgattaagga
     1801 ttatcaaaac gacatttctg aagtgcatta tgcagcagat aatattcaat tagtactacc
     1861 aatacaatat acttcaaaag agttggagga ggaattacat gactgagaaa gaacaagtac
     1921 aacaaatcgt gaagaagtat aataaaagta tcgctgattt atcagaaaac gcctcagcga
     1981 aagaatttaa aacagtaatg aattacgtag cagatgaagc aaatcgtaag cagcgaaaac
     2041 tagttgggtt ggataaataa attttttaaa ccatatcctt gtaaagttga gttttttatt
     2101 ttaatgaatt aagactatta aatagataat tattgtaact taaaaaacac agctaaaaat
     2161 tagttgtgtt ttttattagg tttacttttc tggtata
//
LOCUS       DACESX010000210         2171 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.215, whole genome shotgun sequence.
ACCESSION   DACESX010000210 DACESX010000000
VERSION     DACESX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.215"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..1218)
                     /locus_tag="IXF88_002662"
     CDS             complement(<1..1218)
                     /locus_tag="IXF88_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1509356.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFS"
     gene            complement(1352..>2171)
                     /locus_tag="IXF88_002663"
     CDS             complement(1352..>2171)
                     /locus_tag="IXF88_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1509357.1"
                     /translation="GTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYN
                     PEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIVVVTYPDGS
                     KEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQYHGTADKN
                     GNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDNKTDNQGSK
                     QNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          632 a          413 c          277 g          849 t
ORIGIN      
        1 agaaaacatt gataatagtc cagataaatc ttcttgacta caaagttgta cacctactat
       61 tctctttgta tttttttcaa agctaattct taattttaca cgactatttt ttctcaaaaa
      121 aacaggtcta atgaaatctt ctatttctat ataatctata gactcactta ataatcgtgc
      181 tgtttttaaa ttcaaacctg ctgcgtaaaa attgtaatta aatatagaaa agccatttgt
      241 aaattgggaa ccagaaggtg taaaatcttg attgtcaata tgttgagttg caatataagc
      301 tgttcttaga gcattagaaa tagaaaaatc aacactactt ctattaatgg acgtaaaaaa
      361 agttgtagca caatccccaa tagcatatat atcttttata tttgtttgct ggtattcatt
      421 agttagatat gctccactaa catctctcaa gagttccttt ttgccaattg gactattaga
      481 tagaaatcct atagcttgta cgaccatatc catagagtat tgggtaccac ttgtagtgac
      541 tattttttca atcttttttc ttcctaaata ttttttagga ctttctgata gacaaagatg
      601 tattccattt tttgatagta tttcttcaac tttatctgaa aaactacggt cgtaataagt
      661 cgacaacact ctatctgcaa catcgaaaag atatacttct ttccctcttt ttcttatggc
      721 ttcagcaatt tcaactccaa tatatcctgc tccaataatt cctacttttt taatagtatt
      781 atcatctaaa gctttaaaaa tctctttagc atttggaaaa ttctttatcg aaaaaatatt
      841 agctaaatta ttccccggta catctaacat aatttgcttt gaacctgtag ctagtataag
      901 tttgtcatat tcttgggaaa atcttgtgcc atcttttttt acacaaataa tctttttatt
      961 ttgaatatca attttgtcaa ctgctgtctc caaaaacaac ttttttatct tagaatttag
     1021 ttctcttcta ttttgataaa aaagatcttc taaattgcta acttctcttt ttatgaacaa
     1081 cgatgtccca cattctaagt aactaatttc tgatttttta tctatcagaa taacttcata
     1141 atttgaatca cataagttta gaaatcgagc tgcagctagc cccgaatggt ttgatccaat
     1201 aattactatt tttttcactt aatcacattc ttttcccttc tatgaaaagt atcttcattt
     1261 ttaactaaca acctatctcc caaaataaaa aacaaaaaga ggaagaaaaa gattcttcct
     1321 cttttcagag aagatttaag aaattgattt tctattcatt ctcttttttt cgatttttat
     1381 accctaataa ccctaaagtt cctaacagga ttaaaccacc actaatagta aagatattac
     1441 tatcaatctc tcccgtctta ggataagctt ttgaggactg cttttgagga ctgcttttgc
     1501 ctctattttt gttttgcttg ctaccttgat tgtcagtctt attatcttta ctaacggctt
     1561 tttcactgtc tttttcgttt gaagaaacaa ctacattagt aggttcacta gtattaccag
     1621 attcatcagt tgcagttact atcacttttg ttcctgcctc taatttagga actttaactg
     1681 taaaattacc atttttatca gctgtcccat ggtattgact cccgtttggt aatgtaactg
     1741 tcacctctgc atttggttca gttttaccag taacaatttg gtcaccctct gtaataggat
     1801 caacttgagg tttattcggt gcttttttat cttctacaga aatagtaacc tctacttctt
     1861 ctttagatcc atctggatat gttactacta caatcccttt tttgtttcca gctgtactaa
     1921 catctacttt ctctttccaa gtataagtag ttccttttgg tagatcttct ttgttcttaa
     1981 ttccctcaga tgcgtctggc tctttattta agtctgtagt cactttttgt ccttcaggat
     2041 tgtatttatc ggcatcggaa cgattgtctg tcacttctac tggaactttc actgtatctt
     2101 tcgtaccatc tggatacgtt acttcgataa ccccttcgta attacctggt gtgttggtat
     2161 caatcgtacc a
//
LOCUS       DACESX010000211         2139 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.216, whole genome shotgun sequence.
ACCESSION   DACESX010000211 DACESX010000000
VERSION     DACESX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.216"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(727..1017)
                     /locus_tag="IXF88_002664"
     CDS             complement(727..1017)
                     /locus_tag="IXF88_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509358.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1225..1916)
                     /locus_tag="IXF88_002665"
                     /pseudo
     CDS             complement(1225..1916)
                     /locus_tag="IXF88_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          609 a          395 c          312 g          823 t
ORIGIN      
        1 tatgtttgtt catttttata ctccttctta tatttcctaa tagagtataa catagcttta
       61 agcattactt aaagataact taaaagttat cttttaaata cttaattgtt aaatacctta
      121 tttgatgcaa aagaaatcta cgatagtcaa caaaaaaatt ttcattatat ccagaaaaat
      181 ttttcttagt taaggaccta tggattgcag taatgcagaa ttcgtcgaat aaactgccaa
      241 agacatacaa tcatattgct tttaaaattg acgaacaaga tatagattcg tttgtctcaa
      301 aaatccaaat gttaggttta actgtcgaac caggacgttc tcgtgtgaaa ggcgaggctt
      361 cttcaattta cttttacgac tatgataatc acctcttcga gttacatacc ggtactctcc
      421 aggaacgttt aaaatcctac aattccacta cctgagacta gtaatgacta gtctcttttt
      481 atggcctctg atccccccat tgagcgaata ctaatagggc ttcgaattga tctgttcaat
      541 ctttaagcaa tatccctcaa atgagtttgg aaagtttagt gattcatcac aaatacttaa
      601 atacatgtag ttatatatag attgaggttc ttgtgagcgt atgctcgtgt gggttcaaat
      661 cccatcgtct gcatccatct ttccaaaaca agcggctagg acatttttgt ctcagccgct
      721 tgttttctag tttattcttt catcttgtcg gacctttttt cttgggtata gacttttctc
      781 atattctacc acaattttgg gtttccctaa cacatgatag gatctcacaa caaattgctt
      841 tctattatta taataacctg taacgactaa cttcatccct tcgtctactt cagctaggaa
      901 gttcaaacta tgggaagcaa tcaaacaatt gaccccattt aaactgaaac ggatcaacgg
      961 tctggcacta acttgcaaag tacggatctt atctactgtt ccatattttt tctccataac
     1021 cttcacctca ataccattat acgaacatac attccctttg taaagaagaa actctgtttt
     1081 atctttttga ctttttgtct attacctttc ttaaaatcat attggcactt cctgcgaaga
     1141 tgaaggacat gtactaaaaa aaagaggcag cagtatagat tactgttgct tctttactaa
     1201 tttaggttta cttgttaaga aagattattt acgcaaatgt tcttcaacaa tttgctgaga
     1261 tttatcagct gtatcttcca cttcatcttt tgctttttct aacttttgaa tatattgttt
     1321 caagcttgag ttttcagcag ttgcttcgtc taatttcttt tgtgtatctg ttaactgatt
     1381 tctacatttg ttttttcttg tgtcagtgtt gcgatttgtt gattcaaacc atttacagta
     1441 ttttgtaatt ctgaaagttt tgcttgtgct tcttgtagtc ttgctgtcca atctgcattc
     1501 agatgattga tttcatcatt tttttggttg atctcatcta gtttgacatt gatttcattt
     1561 tgtttgcttt gtagttgtgc atccttgctg actaattgac tttctaagtt ttgtttttct
     1621 gctgccgatt gatttttttg gttttctaat tcatttactt ttgtttggta ttcttggaca
     1681 tctgcctttg aatcttgcaa tgtctgttct ttttgtgcta gtcgagcggt aagatctgta
     1741 atcgcttgct ctttatctgt caatgtatga tcaagctgtg tcagattctc attgattgta
     1801 tcaatatctg tctctccacc ccacaaaata atttcatttg cgtaccgcgt gcctgtgtag
     1861 ccaattagac atagaaatgc aacgacaatc ccaccagtaa taaatttctt tttcatttgc
     1921 atagctcctt aatccaatac ttttacatgc taaaagtaag tgatctcaaa cacaatgtca
     1981 agcgttttta aattacatat gtaattttaa tataaatact atcaaatact catttaatcc
     2041 tcctttttta tctatttaaa aaatcttttt tatagagaaa aatgcaggtt aatactttct
     2101 taaataaata tttatggtga acaaaaacaa aaaagcaag
//
LOCUS       DACESX010000212         2083 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.217, whole genome shotgun sequence.
ACCESSION   DACESX010000212 DACESX010000000
VERSION     DACESX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.217"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            93..2024
                     /locus_tag="IXF88_002666"
     CDS             93..2024
                     /locus_tag="IXF88_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1509359.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
BASE COUNT          712 a          326 c          442 g          603 t
ORIGIN      
        1 gaaaggagtc agaaatagca gaaatttatg tgattcaaga agtaaatgat ggaaaacaaa
       61 ggaattataa attatatagg gaggcaatag aagtggaaac tatccaagaa caacagtctc
      121 aagaaaagca taatcataat cacaaccacg atcacgacca tgatcatggg aaaatgccag
      181 tcgttttgta ctttgttggt ttagcattag cagtgattgc tctatttttg aatgaagatt
      241 atcagttggt acaaaatgtt ttgttctcaa tcgctacaat cagtgccggg tatcacgtta
      301 ttcttcttga aggccttgga gaaacaattg agaattcaaa agcacaaaaa aagttcatac
      361 ctaattctca tattttaatg ggattagcgg caattggagc ttctttgatg gggaattttt
      421 gggaaggaac attattgatt cttatttttt ctggcgcaca ttttttggaa gattatgcag
      481 aaggaagaag caaacgagaa ataacgaaat tactcgaaat gaatccaacg actgcccggt
      541 taatcctgcc tgatggtagt acaaaaaatg tggatgtcag tgaattaaaa gtgggagatc
      601 aacttcaagt gttgaatggc gatcaagtac ctattgatgg cgtcattttg tctggatcta
      661 catccattga tgagtcgtct attactggag aaagtatccc gaaagagaaa tccaaaggag
      721 atggcgtctt cgggagtaca atcaatggaa caggtacctt cacgatgaaa gtcaccaaag
      781 aaaacaaaga tacagtattt tcaaaaattt tacaattagt gaatcagaac caagataacc
      841 aaacaaaagc tgcgagtatt atccaaaaat ttgaaccgaa atatgtcaca gttgttttaa
      901 ttgccattcc tctattcatg ttattggctc cttttctact tgattggaca tggtcacaaa
      961 gtgtctatag aggattggtc cttttagttg cagcttcacc gtgtgcttta gcagcagcta
     1021 ctgtctcggc aacgctatcg acgacttcta atttagccaa aaaaggagtc ctttcaaaag
     1081 gtagttctta cctgtctcaa ttagccgata ttaaagcaat tgcatttgat aagacgggca
     1141 ccttgaccaa aggaaaacct gaagtaacca attattattt tgctgattcc gtgaacgaag
     1201 aaaacatgat tgatatcgta gtagctcttg aaaaagaatc caatcacccc ttagcagatg
     1261 ctattctaag aaagtttgag caaaaaaaca aactaactat tgaagtagaa aaccaaattg
     1321 gtaaaggatt gacgggagat tacaaaggga gaaattatcg tataggaaaa ccgacttcgt
     1381 ttgatgatgt ttcagatgaa tatagtcgtt taaataatga atgggcgtca gaaggtaaga
     1441 cagtcgtata cgtagcagta gatgaagagg ttataggcct tattgccctt atggacgttc
     1501 cgagtgaata tgctaaagaa accattgaat attttaggaa acaaggtata cacaccacat
     1561 taattactgg tgactcagaa atgacgggaa aagcggttgc taaacaatta ggaatagatg
     1621 aagtgattgc gaatgtcatg ccggaagaca aatccagaat tatagacgaa caaaaagaaa
     1681 aatacggcgt gaccgccatg gttggagacg gtgtaaatga tgctcctgct cttgttaatg
     1741 cagatgtggg aatcgctatg ggagatggga ctgatgtggc agtagaggta tctgatttag
     1801 ttttaatgca aaacaattta tctaaattgg tacaatctca taatatttct tcgaaaatga
     1861 accgtgttat ttggcagaat attatttttt caatggccgt tgttgctttt ttaattgtcg
     1921 ttagtttctt aggcttaacg gatattgcaa tcagtgtcat tattcatgaa ggaagtactt
     1981 tggttgttat tttgaatgga cttcgattgc taaaggcagt ctaaagaatg attgcacaac
     2041 ttcaaattaa tcgagtaata gagataatta aggattacac tac
//
LOCUS       DACESX010000213         2051 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.218, whole genome shotgun sequence.
ACCESSION   DACESX010000213 DACESX010000000
VERSION     DACESX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.218"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            75..2021
                     /locus_tag="IXF88_002667"
     CDS             75..2021
                     /locus_tag="IXF88_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ1509360.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT          661 a          339 c          367 g          684 t
ORIGIN      
        1 ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa
       61 aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa
      121 aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg
      181 acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa
      241 ttgatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac
      301 ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta
      361 aagataaaaa attcaacccc attatggcat taattaaatt attatcagat atttttgttc
      421 caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag
      481 ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt
      541 cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta
      601 tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca
      661 tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt
      721 cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta
      781 tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat
      841 tattatcagt aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat
      901 tgttgtctaa tggaattaca gacattattg tttggctata taacactact ggatttattg
      961 gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta catcaatcat
     1021 ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact
     1081 ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt
     1141 ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac
     1201 ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct
     1261 tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg
     1321 ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc
     1381 cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct
     1441 acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag
     1501 ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa
     1561 caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt
     1621 cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa
     1681 atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac
     1741 atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata
     1801 ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa
     1861 aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata
     1921 caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa
     1981 atacagtcac tgagggtact gatttacttg aaatcaaata aatgtatttc cctgagtaaa
     2041 gatggaattt t
//
LOCUS       DACESX010000214         2035 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.219, whole genome shotgun sequence.
ACCESSION   DACESX010000214 DACESX010000000
VERSION     DACESX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.219"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            133..822
                     /locus_tag="IXF88_002668"
     CDS             133..822
                     /locus_tag="IXF88_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1509361.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            836..>2035
                     /locus_tag="IXF88_002669"
     CDS             836..>2035
                     /locus_tag="IXF88_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1509362.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIP"
BASE COUNT          632 a          282 c          404 g          717 t
ORIGIN      
        1 tccattatac tccaaaataa taaaaaaaac tcctaaatac tccattttta tctttacttt
       61 tatgaaagcg gatgctatat tgtttttgga gttgttatcc aaaaataata aaaaattgga
      121 gtgtttagga taatggataa aaagaggttt ttagatctta tttcaaaagg tttgattgtt
      181 tcctgtcaag cattacctgg tgaaccattt tacaaagagg aagaaagttt aatgccttat
      241 tttgctttgg ctgctcaaag ggctggagca gttggtatac gtgcaaattc tgttcgagat
      301 attattgaaa ttagaaagtg tgtatcatta ccaatgattg gaattattaa aagagattat
      361 ccaccagaat ctccattcat tactgccact atgagagaag tagatgaatt agtagaaacc
      421 ggtgttgagg taatcgctct tgattgcact cttagaaatc gacatgatgg actatctata
      481 aattctttta ttactaaaat aaaagaaaaa tatcctcatc agttatttat ggcagatatt
      541 tctacatttg aagaaggcaa aaatgcatat aaagcaggaa tagattttgt aggaactact
      601 ttaagtggat atactgagga aagtaaaaaa caagatgggc cagatataga tttgattaaa
      661 aaattggtag aagaagggat ttgtgtaatt gctgagggaa ggattcatac tccagaacaa
      721 gcaaaagaga taaaaaaaat aggagttgca ggtattgttg tgggtggtgc tattactcga
      781 ccacaggaaa ttgcatcaag atttgttaaa gcattaagtt agggggagaa ataaaatgtt
      841 taaaaaattt tcacaattag gacgcgcttt catgttacca attgctatat taccagtagc
      901 cggattacta ttgggactgg gaggagcgtt aacaaatgag agtgctataa atgcttatcc
      961 atttttagat caaccgtggt tgcatacaat tttaagtatt atgagttatg ctggaaatgc
     1021 agtttttgca aacctagcac taatttttgc aattggtatt gctgtaggac ttgcaaatgg
     1081 agataaagga acagctggtt tagctggtgg agtagcttat ctagtatata ctgcaactat
     1141 tagtgggttt cttgaacttt tttcagcaaa agatgcaaca ctagatacag gtgtagtggg
     1201 atcaattgtt gttggtggta ctgttgcatt tttacacaat cgttatagaa aaattgaatt
     1261 accacaattc ttaggttttt ttggaggatc aagatttatt ccgattgttt ctagtttagc
     1321 tgcaatcatt attggttctt tcttttattt aatttggcca ccaattcaaa atggtttagt
     1381 agttgctggt gaacaaattg cccaaatggg tagtttagga acttttttat atggtttttt
     1441 attgcgttta acaggtgctg ttggactaca ccacaccatt taccctttgt tctggtatac
     1501 atctttaggt ggtaccgaaa ctgtagctgg atcaacaatt gctggagccc aaaatatttt
     1561 ctttgcccaa ctagctgatc caaaccacac tggtctgttt acttatggta ctagattttt
     1621 tgcaggaaga tttgcaacaa tgatgtttgg attaccagca gcatgttatg caatgtatcg
     1681 tgcaattcct aagaaaaatc gtaaaaagaa tggtgggctt tattttagcg gggcccttac
     1741 atcattttta actggaatta ctgaaccagt tgaatatatg tttttatttg ttgctccttg
     1801 gctttatgtg attcatgcat tcttagatgg attatctttt tattttgctg atatcttaaa
     1861 tattcgtgta gggaattcct tttcaggtgg tttgatcgac tttttattat tcggtgtact
     1921 tcaagggaat gacaaaacta attggttaaa attaattcct tttggtattg catgggcatt
     1981 tatatatttc tttgttttta cattctttat caaaaaattt aaggtagcta ttcct
//
LOCUS       DACESX010000215         2031 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.220, whole genome shotgun sequence.
ACCESSION   DACESX010000215 DACESX010000000
VERSION     DACESX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.220"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(434..976)
                     /locus_tag="IXF88_002670"
     CDS             complement(434..976)
                     /locus_tag="IXF88_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509363.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            complement(1289..1840)
                     /locus_tag="IXF88_002671"
     CDS             complement(1289..1840)
                     /locus_tag="IXF88_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1509364.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
BASE COUNT          607 a          391 c          371 g          662 t
ORIGIN      
        1 ttaagattaa tgacactaaa ttaacagact atacgtagta tctatttgct atgaccatat
       61 cttatatcca taagtcaact cagccggact aaaagggcta atcttactag aacacaagct
      121 gcacttgata tgcaaattaa ccctgaatcg gaaaggtgct taaattggat attaacggag
      181 ggataaagta aaatttatct ctagggaatg tttagaaagg atccaagttc cttaacaagt
      241 gacaattagt aatgtagtaa caacgatagg ttgtatgccg aacatttcaa tttgatacgt
      301 ataaaagacg atttcaagct catccgtgat aaataaccca catgaatgat ttgaaatcgt
      361 cttaaaatgc tttattttat aacccaaaat agaataatta cttcaaaatg acgtcgcata
      421 tcactattaa tgattaatcg atgacttcca taatcatttt aagttccccg gatgttaaat
      481 cctcgctaaa aatgccagta actttttgtc caggtccaat ttcctttgat gataggttta
      541 acaaagagat agggatatag attaatccgt tttgtttcaa agacagagcc aatatttttt
      601 tccttgttcg aatagctgca tcatagcgaa gagaagagta aacacctctg ccaacaatag
      661 aaatatgctt ccccaataat aaatcaaggc tacctattac ttgtaatttc tgaaaggtcg
      721 tcccatccgt tttgatttga atatcctgta aatttatttt cctgtctaag gcatcttgaa
      781 aaaacaagtg tgtgcctctg cgataatcaa tgccacagag gtgcataaaa ttagttgtcg
      841 aaaaaataat cgctaccgtt gagacttctc cgacaaagga tactttttta tctacaaaat
      901 gttcttgtaa gagatgagca gccttataaa tcgcgtttaa ctgtctggta atttgggttg
      961 tctcttttat agacatgcgt ttgtattctt tttttgccgc gtgcgaagaa tgctcctttt
     1021 tcccaataaa aaaagctgct caaaagagca gccttttgta caggtttagt tttggtgctt
     1081 gccacccgcc ggacccttac gttccggatt tgtatcgagt ttagttttgg tgctcgccac
     1141 ccgccggacc cttatgttcc ggatttatat caagttgact atgagcctgc tctcatttgt
     1201 atatataata taccaaaaaa aggttgattt gtccaatact gtgggtgatt ataaccgtct
     1261 tactgtgctt atctattata taatacaatc aaaaacctaa ccgaaaattt aataacgttt
     1321 cactgatttc atcttcatta attccgatgt agcgtttggt aatcttctcg ctgctgtgac
     1381 caaagatttc cattaaggtg gccacgtctt tggttttctt gtaatagtgg taaccaaagg
     1441 tttttcgtag ggtgtgggtg ccgatatcgt cccttcctaa cagcttcgcg accttttgaa
     1501 acatctggta gaccgtattg acttctaaat ggccgccctt agtactggga aacaagtaag
     1561 cctccggatc taagggtgcg gtatagccgt gaatcaagtc ctgtaagctg cttaagtata
     1621 aaatgcgtgt tttccctgtc ttttgctcca cgatgcgcgg gttttttgag gtaatcacgt
     1681 cttttttctt gagtttaacg atgtcggaca tgcgcagacc gctgttaatt ccaattagaa
     1741 acaagaaaac gtcacgttcc gcatttttat tccgtcgcag gcaaaataaa aaatcattaa
     1801 tctcctgttg cgaccgtagt ggctgaacat tatagcccat gacaatccct cctttttagt
     1861 ctgttcgaat acatgattat agttattaca acacgaaatc gtgtgtttag ggggagtaaa
     1921 aaaaaagaac cctataagga taggattctt gatacatgat tttatccaat ttcatgtatc
     1981 ttatattcaa cccgcgccac aagagtatgg ttgatatatc attttgactt t
//
LOCUS       DACESX010000216         2028 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.221, whole genome shotgun sequence.
ACCESSION   DACESX010000216 DACESX010000000
VERSION     DACESX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.221"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..1192)
                     /locus_tag="IXF88_002672"
     CDS             complement(<1..1192)
                     /locus_tag="IXF88_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HAQ1509365.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKN"
     gene            1213..1986
                     /locus_tag="IXF88_002673"
                     /pseudo
     CDS             1213..1986
                     /locus_tag="IXF88_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          614 a          406 c          328 g          680 t
ORIGIN      
        1 aattctttat gtactctgta acatcgaata catcttctaa taaaaatgca cgcaataatc
       61 tgtcaattag catatttacc ttgattggta ttaccatttt acctaaattt ttctcgatat
      121 atccactgaa atgacctacg ttaacacttg attttaattt atcaaatcga ctatattcat
      181 taatgtttac ctcggttaag cgtaaatcca acatctcacg taataatgcc tcattaatat
      241 tggttgaatc cactaaatct tggataaaat cgtcctcttc actaagctga tattcattga
      301 ggtaatcagt aaattcccat ccttctttga taactaattc cccattattt acatcattta
      361 gaatcatctc agcatatggc tgtaactcca ctggtaattg cgcaaatgtg cgatgtaagt
      421 cgtttaacaa tgattgaatt aaattcgaat cacgttcttc ttttatattc atgtattttt
      481 tgaagtttgt gtttaaatac tcataattaa tacgactacg actaccttca ggtaattggt
      541 aatcaatgtc atacgctata ctatcatttc cgtctctagg ttctctctct atctcgctgt
      601 aacgttgtgc caagatatta aattcatatt ctgtgattac tactgttata ggttcataat
      661 cctggatttc ataagtcgat tttgcccatg tgaatccctg tatcatcgct gattcataac
      721 aatggattag ttgattataa agatttacaa attgcttaat ttcccctgga acctccggta
      781 aatgactgaa gttttctatg ccagaaatat taaatacgga cttaatttct tcgaatatat
      841 cgttcaactt ttctaagtta ttatataatt tttcaacaaa taaccctttt gcttgtttgc
      901 ctgagtataa ctcaatagcc tcttcaatgt tctgttccat cgtatgtgct ttacgataat
      961 aacgaatggt tccgaacggt ttttcaggac cattaagacg atttgtgcgt gaaaaagcct
     1021 gaattatgtt ctgatattca agtgacttat ctaagtatag ggtgttgacc cattttgaat
     1081 cgtaacccgt taagagctga ttaacaacta taacaagatc taattgggtt gcttcatcta
     1141 ctgttttgta aggattctta tgcgctgtgt tttgcaatag ttttgtacac aagattgcaa
     1201 agaaaagtac acaatgttgc cacttctggt tccatgagtt ttatttgtgt tgatgggaca
     1261 accaatcagc tatttacgac ccttgaagat cgcagacttt acaaactaac tcaatacttc
     1321 ctgcgctttc ctagaaaggc ccgtttaaag gtcaagtacc tagtgatgga catgaatgcc
     1381 agctattgtc agttgcttaa aacggtcttt ccaaatgcgg agatcgtaac tgaccgcttt
     1441 catattgtcc aacacatcaa tcgcagcttc aatcaattac gtgtccagat catgaatcgt
     1501 tttcgaacct cacactctga aaatcagaag aagtaccgtc ggctcaagcg atattggaaa
     1561 ctcttactca aagattccac gacatgggag gcactcaaac ggcattatca tcgactgttt
     1621 aaacgaccaa ttagtcaaac agaaatcgtt aatgagctgt taagctacaa cgaagaattg
     1681 cgaactgcgt atcatttttg tcaattatta cgctattatt tcgttaaacg agatacggta
     1741 ggctttcaag aaacccttaa aacaatttca tctacgttac ctcagtcgtt taaaaagaaa
     1801 ttcacgatct tccatcgcta ccaatcgggt atttcaaatg cctttaaggt gtcccactca
     1861 aacggcgtca ctgaggggct gtataacaag atcaagctga ttaaacgaat tgcgtttggt
     1921 tatcgcaact tttataactt tcgggcacgg atctatttac aacaaggtct gatctttgaa
     1981 aactaaaaaa atatgcggcg cacagaacgc gccgcatatc ggactaag
//
LOCUS       DACESX010000217         2012 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.222, whole genome shotgun sequence.
ACCESSION   DACESX010000217 DACESX010000000
VERSION     DACESX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.222"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(85..954)
                     /locus_tag="IXF88_002674"
     CDS             complement(85..954)
                     /locus_tag="IXF88_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1509366.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(1006..1998)
                     /locus_tag="IXF88_002675"
     CDS             complement(1006..1998)
                     /locus_tag="IXF88_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1509367.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
BASE COUNT          570 a          461 c          329 g          652 t
ORIGIN      
        1 agaagaatga attgagacta tttaagtttc agaataccct tttaaacttc cggtatgtta
       61 tgaatagttc taataaatac tattctaatg attgagtttc tttattgcta aagctaggca
      121 gccaatcaag cccgcattgt cagcaagcct tggagttagt agatattctt cgagagtagg
      181 cgtatccacg taggaaccca ttttttcttt aaacgattgg cgaatcaact cgagtagttg
      241 cggctgtttc attacacccc cgcctaaaat tattacttcc ggtgcatata gtaacgttgt
      301 gttatacaca cactgagcaa gataatctgc ctcaatcgtc caataaggat gatcaaatga
      361 gatagtctgg gcttttcttc caaagcgttt ttcaatcgca ggtcctgatg ctaacccttc
      421 caaacaatca tgatgaaaag gacagattcc ttcaaattga tcttcattat gacgttgtaa
      481 gagcatatgt cccatttcag gatgactaaa acctgcgact attttcccat ccactacagc
      541 accaccacca acacctgtac caacggtgta atacagaaca ctatttttac cttttccata
      601 ccctgccata tattctccat aacaagcagc atttacatcg gtagtccaat agatgggaac
      661 cgacaaggcg ttttccaaag ctcctacaaa atgataattt tgccaagcta acttcggtgt
      721 gttggtgata tatccgtaag ttcttgattc gatttgaata tcaataggcc caaatgctcc
      781 tacaccaatt gatgcgatat ttcctttgta cttatcaaag aaatcaaaca cttgctccat
      841 cgtttcttct ggtgttgtgg taggaaaact tttccgttct atcacttgta attgttcatt
      901 accaatccca cagacaaact ttgttccacc ggcttctatt ccaccatata acattttatc
      961 tacacctttc attcactcat tttcaaaaaa agaaacgtat ttgcactaag caaaatcgtt
     1021 tctcactaaa ggtttcgttg tgtcaccttc gccaaattca atcggcaata cggtacgttt
     1081 ttctactgtc tctccattaa tgacagcaat taattttgcc actgccactt ccgccatttg
     1141 ctttgtaggt tgggcaatcg ttgagactag ataaggatct ccttctgtca tcttttgacc
     1201 atcaaatccg ataacttgga catcctctgg tacgtttttc cctatttttt caagcatatc
     1261 aatgacagct aatgccattg gatcgttgat cgtaaaaatc ccatcgatat cgggattctc
     1321 taacaaaaaa tttgttacac gttctgagag atggtctaaa ggttctaaca tatctaacgc
     1381 tttataggaa atccccagtt gacggcactc gatttcaaaa tattttcgac gactcttcgt
     1441 ttcattcggt gtttcttggt acccaccgat atatgctaca ttctgacatc ctcggcgata
     1501 aagttcattt gcagccaaac gtccaccggc cgcattgtca gcggtcacat aaatcacctc
     1561 ttcagtgaaa taacggtcaa tactaacaaa aggcagatta ctcgaaatgt attcatcgat
     1621 atctgaataa gtaattccaa tgatgccgtc tactttattt tggctgacca tctggatata
     1681 ttcttgttct ttttcactat taccactgga gttacacaaa taaagcttgt agttttgtct
     1741 ttctaatgct gtttctacat ggtaggcaaa ttcgccaaaa aatggatgcc ataccgtggg
     1801 tagaatcaaa gcaaccgtgt tcgtatgctt catctttaac ccccgggcat aaaaatctgg
     1861 ttgatagttt aactctgtca ttgctgcttc aactttttct ctagtagaag ctttaacaga
     1921 accgttattc aatacccggg aaacagttcc tactccaaca cctgctaatt ttgcgacatc
     1981 tttcattgtt gctttcatag ttgatccctc ac
//
LOCUS       DACESX010000218         1999 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.223, whole genome shotgun sequence.
ACCESSION   DACESX010000218 DACESX010000000
VERSION     DACESX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.223"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..374
                     /locus_tag="IXF88_002676"
     CDS             <1..374
                     /locus_tag="IXF88_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ1509368.1"
                     /translation="VSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRRF
                     PEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDDW
                     QKVMAAEVIATYLDGDLVYKK"
     gene            422..1822
                     /gene="yfcC"
                     /locus_tag="IXF88_002677"
     CDS             422..1822
                     /gene="yfcC"
                     /locus_tag="IXF88_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1509369.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1855..>1999
                     /locus_tag="IXF88_002678"
     CDS             1855..>1999
                     /locus_tag="IXF88_002678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1509370.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          551 a          339 c          432 g          677 t
ORIGIN      
        1 atgtttccac agaggacttc tttaattatt gtcgattaga ggaaagtggt gtaacagtca
       61 ctagcggtac agatttaccg ctgtttatta ctgatattcc tgaatcgatt ataagagcag
      121 tattccgtcg ttttcctgaa ggagaagaag tctgggagaa agaaacaagc ttcacgacaa
      181 ccgagctgct gaaaagtttt acaagcaaca gcttcaaagt aaatggttta tcggagtacg
      241 gagaaataaa agtagggaaa agcgccagct tagctgtttt cagcgaggac ctctgtactg
      301 atgactggca aaaagtgatg gctgcggaag ttatcgctac ctatcttgat ggtgacttag
      361 tttacaagaa ataatgcttt tcgatataac gttataaaac gaattaaaga gaggttaaaa
      421 aatggaaaca atgaaaagaa aattcacctt cccacatagt tatacattga tttttatctt
      481 aattattttg gctgcaattt taacttggat aattcctagt ggtcagtttg agacagttgt
      541 tgaaaacgtc gcaggtatgg aaaaaacaac ggttgttccg ggaacctatc accaaatcga
      601 taaggcagat tatggacaag gtttttcctc tatactaatg gcaccagctg aagggattat
      661 ttcagctgtt gaggttgttg cttttgttct catcgttggt ggtgccttcg ggattatcct
      721 aaaaacggga gccatcgatc gagggctgtt taccttagca aaagttctgg gagacaaggg
      781 gatcttagtt attccgatgt cgatgattct attcagctta ggaggatcaa cctttggcat
      841 gagcgaggaa gtcattccct tatttgcggt gtttatttct ctgatgtttt ctcttggcta
      901 tgattccatg actgcgatct tgattctatt tttaagtaca caagcgggat atgttgggtc
      961 gacgattaat cccttcaacg ttttaatcgc acagggagta gctggcgttt acggaaatcc
     1021 cctgctttgg tttcggatgc tatcctggat tgtcattact gggatttcaa ttgttttcac
     1081 catgctttat gctagcagag tcaaaaagaa tccactgtcc tctattgttt atgagagtga
     1141 tttaaaggtt aagcataagt ttcaaaattt tgacactgaa aaagagtttt caacacaaga
     1201 taaactaatt atcgccggtt ttatcgtagg tttaggaatc atgatttggg gtatcatttc
     1261 caagggatgg tatatggttg aaatttctgc actattctta ggattaggtc tttttgctgg
     1321 gggcatcgct agattttctc aaaaggaaat tgctgaagct tttgtcgaag gctgttcaga
     1381 tttcgcctat gctgctgtaa tcattgggct agctagagga gtcttggtaa tcttggaagg
     1441 tggaatgatt attgacacta ttctaaacag cttggctaac cttttaggtg gtcttccaaa
     1501 attcctgttc actacgattc tactcatttc tcaagtaatt gtttcctttt ttgttccatc
     1561 ctcctcagga gctgcggcat taacgatgcc ggttatggca ccattgggtg atctaatgaa
     1621 tgtaagtcgt gatgttgttg ttctaagtaa ccagtttgga aatggtttaa tgaatattat
     1681 ctccccaact agtggtgtat tactggcagg attagccatc gcagagatta atttctctaa
     1741 atggtttaag gttggcttca aaatatttgc catcttaacc cttgtttctg ctgttttgtt
     1801 atacattgct acactctttt agtcttaatc atccattaat aggaggcaat agttatgatt
     1861 aaaataaatc aattagcagg aatgaatatc cactatttat tttattctct caaagaattt
     1921 ttcgcttatc aacagaaaat tggtatttct tctgttgaac tttggggagg tgctccgcat
     1981 tttttcatag atgctttta
//
LOCUS       DACESX010000219         1923 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.224, whole genome shotgun sequence.
ACCESSION   DACESX010000219 DACESX010000000
VERSION     DACESX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.224"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            326..1654
                     /locus_tag="IXF88_002679"
     CDS             326..1654
                     /locus_tag="IXF88_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509371.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            1665..1874
                     /locus_tag="IXF88_002680"
     CDS             1665..1874
                     /locus_tag="IXF88_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509372.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT          634 a          315 c          354 g          620 t
ORIGIN      
        1 aagtttggct ggtaagtctt tttttgagct taacttactt ttgtttcttg tcttttttct
       61 tttcattttg tggctgtggt tttagaaatc gatcgatctc ttcttttggt gcaactgttt
      121 tttccggatc gacagcaaat gattcgaaaa gtttatctaa attatctgta cgttcaaatt
      181 tcaaccccat tgtaaacacc tctttgtttc tttttactta gtctatcatg taatcgtcca
      241 aattggtttc taagacataa agttttagtt tttctttatc gttggttata attaaattat
      301 aaaaagattt taaagaggtg agaaaatgat tggcgaagat aaattattga tgactatcca
      361 gctcacagac ttaaatgaag aagaaaaaaa acaggaaata agcgattggg ctattttgac
      421 acgtctgttg atccagccaa catttatccg ctctttcaca caacaagcgg atccgtatga
      481 tcttcgaaag cttcaagaaa aaataatgct agcatcggta agagacgaaa gttggttagt
      541 tgtaggaaat gacgagaacg aatgtagttt tcgcttggaa gataatcaat tattgataaa
      601 aaatgtattg tctatctctg ttttcaaaga aaagcaattt ttgattcgag acttcattca
      661 gaaaaaaatg attgctcatg gggtgttcgc ctatatgcga gcttatagcg agtttatcta
      721 tcacaataca aaacagatct ctcaacggct attgtttgag aaaaaggatg aaatcgaaca
      781 tttacctaaa atgaaacaac aaaacgggga agtagtagtc gattgcaacc aatttccagg
      841 atacgatctt ttttaccaag gtttatgttt cacttcgtgt tgggaaatgt attatagtcg
      901 ctattatcat caaatcatcc ctaaaccgat ttttctcgac atccaacaag tagaaaaagt
      961 gaaggaatta gaaaacgaag tcatatgtat ccagctttat cgagacccct ttaactggca
     1021 aaagccaaac aatcaaatgt ttcaatccta ttttagggac cagcttgggt ttgatcatct
     1081 tgcttgggac aatggtgtcg gtttattgaa accgccattc gttgaataca tttacactga
     1141 ccatacgatc caatctgtcc agtatcagaa tgcacaaatg caacctgttc ctaaaaagaa
     1201 tgcttcattt tttgtaacaa aaagttatga tattcaacaa ggagattata aagaaaggcg
     1261 tgttcgcgga acactaaatg ctcaggcgta ttttccttgg gttgacgaga atcgctcgcg
     1321 catgatgtgc tataaagtta tcgatccgac tgttgctttg gataatggaa tcgaagctta
     1381 ttgttattat attagggaat atctagaagt agaggtaaca gatgaaaaat atcaagagta
     1441 tcttttatcc ttaagaatct atgttccttc agaatccctg tcagaattac cgttaaagga
     1501 aatcaaacat agactctctg atgttacctt caaacgaata aagaaaaagc ggcgaagtat
     1561 ccaattcgat gtcaagaaag gtgagcagca tctgcgtgtg caatttttag attaccgaga
     1621 attagaacaa ttgattactt tacaaaaaat atgaggtgaa gaaaatggat aaacaactct
     1681 attatcgaat aatggttccg gcatcattat tatccgtctg tctctttttt gttcctaatt
     1741 ttcctattcg atcagtcatc ggttttttgg tcatgtttgg cgggatggga ctttattatt
     1801 atcttcttca taaggaaaga aaacctgctt ctgtttatat gaaaaaaaca agaagcgaaa
     1861 ataaaaaacg ataaaacggc ttgatgatag acaaaaaaat aaaaaaccag tctttttgac
     1921 tgg
//
LOCUS       DACESX010000220         1904 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.225, whole genome shotgun sequence.
ACCESSION   DACESX010000220 DACESX010000000
VERSION     DACESX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.225"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(44..970)
                     /gene="cysK"
                     /locus_tag="IXF88_002681"
     CDS             complement(44..970)
                     /gene="cysK"
                     /locus_tag="IXF88_002681"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1509373.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1110..1904)
                     /locus_tag="IXF88_002682"
                     /pseudo
     CDS             complement(1110..1904)
                     /locus_tag="IXF88_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
BASE COUNT          523 a          436 c          331 g          614 t
ORIGIN      
        1 aaaagttttt tacataaacc tatctggaga aaaaccaagt gccttatatc tcttggtaaa
       61 gggctgtaga taaataacgt tcaccattgt cgggaaccac agctaatacc ttcttgcctt
      121 ttcctaattc ttttgcgact tttagcgcgg cagcaattgc agcgccagat gagatgccta
      181 ctaaaatccc ttcttttcgc ccaacttctc tagctgtttc catcgcttcg tctccactaa
      241 tagaaaggac tttatcatac acagaagtat ccagtgtttt aggaacaaaa cctgtaccga
      301 ttccttggat tttgtgaggt cccgcttctt tcccttctaa aatagcagat tctgctggtt
      361 ctaccccgat taattcgatt ttcggataca cacgttttaa ttctcggcca gcgccagtga
      421 tcgttcctcc tgtaccgata cctgcaacaa acgcgtccaa gccatttact ccaaatgctt
      481 gatgtatctc aggaccagtc gtcttctcat gcacaagagg atttgcttca ttttcaaatt
      541 gtaatggtaa aaagtagccg ttttcttttg ccaaacgttc tgcttctctg attgatccag
      601 aaataccatc cgcaccagga gtcaaaatca actctgcgcc gtaccctttc atcaataaac
      661 gtctttcgat actcatcgtc tcaggcatca cgatgattac tttatatcct tttgcaactc
      721 ctaccatcga caatccgatt cctgtatttc cagaagtagg ttcaatgata gtatcacctg
      781 gtttcagtag accatcgtgt tctgcttttt ctatcatact taaagcaata cgatctttta
      841 cacttcctcc tggattaaaa aactcaagct ttacaaaaac atctgctgaa tcttctggta
      901 cgattttatt taatttgacg attggcgttt tgccaatcaa ttcggtgact gagttataga
      961 tagctgtcat cttcaattcc tccttgtctt tgctcttctt acttatcata atacaatctg
     1021 ttcatttatg gcgagtgatt cgtctttgac tagcaaaaaa aagacaaaga tataccatat
     1081 atggtagttt ctttgccttt ttatctaaat caaacgttta gttcttgacc aactaataaa
     1141 gaataactgg acatagacat gccatttttc gccattagat catcaacaga catgccaaat
     1201 ttttgtgcaa tatcccatag cgtatcacct gacacgactg tatatgtctg accattattt
     1261 gtatttacat ctgttgttga agatgatgac gttgaagtcg tcgtagtagt cgttgttgtt
     1321 gtacttgtgg tagccgaacc tgttgatggc gtatcaaaac gggttaaatt ataagtatta
     1381 atcaatgagt tcaatttggc cccataattt ggatcggtag cataacgacc tgtcaagtac
     1441 gctgttgcat cataaaaact tgttgtattg cttttccaca cgccagcata atgataatcg
     1501 cccgtggaaa aactggttga acgaagaaca ttcgcatggt cttggaagga ttcccaataa
     1561 gaagaatatt ttcggaatgg ttcattcatc gttacccatt gtccgttaca tattctttgg
     1621 ttgccatata aacgacctgt ccgttatagc ttcctttcac accgaataga ttataatttg
     1681 gcgcactagc taatccggaa gtgccatatg cactttccaa caacgcttga gcgatcatga
     1741 ctgaagcata caagtcatta cttgcagcaa cttctgacgc agaccagccg atttgatcga
     1801 taaacgcttg aggatcagca actgtttgag atccttgttc atctgcatga acatgttgcg
     1861 gtaccgctgt aaccatcatc ggcccaacta aaagactcgt tcca
//
LOCUS       DACESX010000221         1900 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.226, whole genome shotgun sequence.
ACCESSION   DACESX010000221 DACESX010000000
VERSION     DACESX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.226"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            54..1003
                     /locus_tag="IXF88_002683"
                     /pseudo
     CDS             54..1003
                     /locus_tag="IXF88_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(1048..1681)
                     /locus_tag="IXF88_002684"
                     /pseudo
     CDS             complement(1048..1681)
                     /locus_tag="IXF88_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT          608 a          352 c          303 g          637 t
ORIGIN      
        1 ttttttttgc tatttattta agggatagaa gtttagaaag gactgtttat aagatgaaaa
       61 atgatatagt gacttatgca aacacaaaat gtgaagaaat tggcatcagt aatttagaag
      121 agtgcttttt tgacacaaat gacctgtcgc aatttttaag aacgggaaga acaaatgcca
      181 gtaaacttgc caatgactta gttgcaaaga acgttctatt taaaatcaat actcgtccag
      241 tgttatttgc catcaaagag cgctttgaag agcacttttt gattccggta aaagatgtat
      301 acgaaagtat atctgaatta aaaacagact tggggcccaa ttctacagat tccattctac
      361 aaaagatgat tgggtatgat atgagcttat ccgatgcggt ggaacaaatg aaaactgctg
      421 tcttctatcc tggaagaggg cttcctatga tgatcactgg agatactgga gttggaaaaa
      481 gtttttttgt tcatactgct tatcagttta tgaaaaaagt gaacgttatt catgagaatg
      541 ctccttttaa agtacttaat tgtgcgcaat accataataa tcctgaatta ttgtcgggga
      601 tactatttgg ctatgttaaa ggtgctttta ccggcgcaga tgaagatcga aatggattgt
      661 tgcaagatgc agacggtgga gtactgtttt tagacgaagt acacagattg cctcctgaag
      721 ggcaagagaa actatttgtt tatatggatg gcggaactta ttctagagtc ggtgaaacta
      781 tccatcacgc ctccaatgta cgtttagttt ttgcgactac agaaggcaaa gaggattttc
      841 tggaaacctt tatgcgaaga atccctatac atatccatat cccaaatatt gaggaacgag
      901 gtattttaga aaaaaacaaa ttatcgaaaa tactctcttt agcgaaagca aaaactttag
      961 caaggctatt gatgtttctc ctaaagtcac tagtgttgca taaatatagt cagaatattc
     1021 tgaattatta ttttcttgaa aaaagtaaaa aaaatcgtat cccaaacttt catctacatt
     1081 tccagaaaga tcttcatgtt gataatcaat tcctgaatca atgaccgcta ccttcacatc
     1141 cttagaaccc ttggtttctt cccaagcgga tggcatgcta atctctctta aattagcttg
     1201 tttactccaa ttaggatcgt tatcgatttc ctaaagaatc agttgtattt ggctcctcaa
     1261 catcattcaa cggctctagt tcaaccacat agtttggctc tgcataattg accatatcag
     1321 aagcattaaa tgttcttact gcctcttcca cactaatttc ttcgttcaat ttcactaata
     1381 atacttgatc tcgcttatta taatcgcttt catttgtcct aagagtcaaa tctttttcca
     1441 ccataacttt tacacccaat aaattttgcg gtttattttt ctctgttgaa attctttgtg
     1501 tagcctttaa actgacaatt agctcattgg gtacatactc tatttgagag tcatctaatt
     1561 ggctcataat cctttctttt tggttagtat ctgcagttac ttgtttagta tgagccatga
     1621 acccaaacat actcacccca acaaccaatc ctaaatatat tttcttttta aactttttca
     1681 tctacatctc ctcctaatag ttttatttta gagttagctc tattacttat ttactaactc
     1741 aacaacagat tatcatacac ttattaatga aatcacaata tctcattaaa acacacccta
     1801 aaatcataac cgttcctatg ctcatttttt ggatttttta atcacgtaac cagaaaaata
     1861 aatcccctct atttcacgca aatcaaagaa gaactaagta
//
LOCUS       DACESX010000222         1891 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.227, whole genome shotgun sequence.
ACCESSION   DACESX010000222 DACESX010000000
VERSION     DACESX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.227"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            13..984
                     /locus_tag="IXF88_002685"
     CDS             13..984
                     /locus_tag="IXF88_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1509374.1"
                     /translation="MEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWMYLLLLPG
                     IIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLLLSNTLKI
                     SIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLFLFLSANG
                     PINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVSTDLYEAA
                     NIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNIPASEIIS
                     TYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            1000..1884
                     /locus_tag="IXF88_002686"
     CDS             1000..1884
                     /locus_tag="IXF88_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1509375.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
BASE COUNT          549 a          318 c          393 g          631 t
ORIGIN      
        1 gaggagattg aattggaaaa gacattggga ccaggtggtg agataagggc ggtacaacgg
       61 gataaaaaaa atatttcgaa gtgggaaaaa ttaaagaaac agttgcggcg tgataagtgg
      121 atgtacttgt tgctcttacc aggtatcatc atgacgttta tctttaagta tgtgccaatg
      181 tacggtgctg tgattgcatt taaagactat aatcctatgc taggaatttt ggaaagtcct
      241 tgggtaggac tagatcactt tagggaattt atcagttcac caaattttac gcttttgctg
      301 agcaacacat taaaaattag tatttatggt ttattgttag gtttttttcc accaattatt
      361 ttagcactga gcttgaatca aattatcaat aaaaaaataa aacagaaaat ccagttgatt
      421 ctatatgcgc cgaattttat ctcagtagtt attattgtgg ggatgctgtt tttgttccta
      481 tccgcaaatg gacctattaa tgggttagta gaaaaggtta cggggcaacc aattatgttc
      541 atgtctaatc cagcttattt cagaacgatt tatattttat cgggaatttg gcaaggaatc
      601 ggctggtcat ccattcttta caccgcaacg ctggcgggag tcagtaccga tttatatgag
      661 gcagcaaata tcgatggggc aaatatttta caaaagattt ggcatatcga tttgcctgca
      721 ttgaggcctg tgatggtgat cagcttcatc ttggccgccg gcggtattat gaatgtagga
      781 tatgaaaaag cctacttgat gcaaacttca atgaacatcc ctgcctcaga aatcatttcc
      841 acttatgtat acaaagtcgg cttgcaatca ggtgattatg cctactctgc tgctgttggt
      901 ctgttcaata tgattattaa tgtcgtttta ttactgattg tcaacaatat aaccaagcgg
      961 ctaaacgatg gtcaaggctt gtaatgaaag gagaaaatta tgaatagtgc tttatcaaaa
     1021 atcgaccgtc aaatcttatg gttgaatcga gtgattgtaa cttttttagt cgtcatcacc
     1081 attgttccgc tgatttatgt actggtagct tcttttatgg acccagctac tttgatcaat
     1141 aaaggtataa gcttcaatcc gaaagattgg acaatacagg gatatcaacg ggtatttgca
     1201 gatgattcca ttttacgcgg ttttgtaaat tcgttgtttt actcttttag cttcagctta
     1261 ttgactgtat ttattacgat gatcactgcg tatcctttgt ctcgacctaa cttggcgggg
     1321 aaaaagatta ttatgacttt ttttgtcatt acaatgttcg tgggtggtgg cttgatacct
     1381 acctatttat tggtaaagaa cttgggcatg ttagatagtg tatgggcgat tatcgtacca
     1441 ggtgctataa atgtgtggaa tattatctta gcccgtacct attttagtgc tttgccaaaa
     1501 gaactttctg aagctgctac tatcgatggc gccaatgact tgcaaatact gttcaaagtc
     1561 atgcttccat tagcgaaacc aattatgttc gtattgtttt tatatgcctt tgttgggcaa
     1621 tggaactcat actttgatgc catgatttac atcaaagatc ccgacttaca gccgttgcaa
     1681 ctcgtattaa gaaatatttt gatccaaaac caaccttcac aagacatggt gggagccaat
     1741 actgccatgg cagaaatgaa acaaattgct gagttgatca aatacgcaac gatcgtcatt
     1801 tctagtctgc cattaatcat aatgtatcct ttcttccaaa aatattttga taaaggtgtt
     1861 ttagttggtt ctgttaaagg gtaagtattg t
//
LOCUS       DACESX010000223         1877 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.228, whole genome shotgun sequence.
ACCESSION   DACESX010000223 DACESX010000000
VERSION     DACESX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.228"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..148
                     /locus_tag="IXF88_002687"
     CDS             <1..148
                     /locus_tag="IXF88_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1509376.1"
                     /translation="KEPEQIFLIEKFIFNKTDKEIGKVLGITRQGATNLKHRLYKKIQ
                     KMLV"
     gene            246..437
                     /locus_tag="IXF88_002688"
     CDS             246..437
                     /locus_tag="IXF88_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1509377.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            951..1283
                     /locus_tag="IXF88_002689"
     CDS             951..1283
                     /locus_tag="IXF88_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1509378.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1580..1735
                     /locus_tag="IXF88_002690"
     CDS             1580..1735
                     /locus_tag="IXF88_002690"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1509379.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          692 a          310 c          321 g          554 t
ORIGIN      
        1 gaaagaacct gaacaaattt ttcttatcga aaaatttatc tttaataaaa ctgataaaga
       61 aattggaaag gttctgggta ttacaagaca gggtgcgaca aatttaaaac atcgacttta
      121 caaaaaaatt caaaaaatgc tggtttaata tctgcataat aactaatttt gtatgcttct
      181 ttgctcgtcc tcctccttcc caaaaaaata aatagattat aagttcatgg gaagaaggta
      241 aatttatgaa tactttaaaa gaagatttca ttttagcaaa gaatggaaat gaagaagcag
      301 ttgtggccat tctcaaaaga tttagcgctt tggtccataa gcaatcctgg agaactggaa
      361 aatatgatca agattgttat caagaatgta tgcttgcaat atatctagct atctcgaaat
      421 tcgaaattaa agaataactc aactaaggga gtctgtgaca taagtaaaaa gatagtttca
      481 aatttcgaac aataaatttg aagccatctt ctttataatt ataaagtatc gattttacag
      541 cgttctataa tgattcatag aatctaaaac gaatgatttt aattattaac gttttttaat
      601 tcgtttttag cttaaaaata acttttgtca cagtctcact cctaaaatta ctttatttac
      661 gtacgaagtg ggcaggaggt cgaatccaga actgggccat ggtacttcaa cgaggacttc
      721 tcctctagaa ttgaaaagca tgcgatttac cttccgtaac gacggtagat ttattcaact
      781 atgtgtccaa tcaaaatttg tctgatttca aaaatagtca agagaatttt ggttgttaaa
      841 aactcttatc tatcaacttc cacagtttag ttgacaaacc ctgatactaa gttgggctat
      901 gatacacttt tcttgactat cagttacgtt accggaagga atgaactcta ttgaaaaaaa
      961 catatgatcg taaatttaaa ctaaagattt cacaggatat ccttgagaaa aaagtaacta
     1021 ccaagaaaat tgctgaagaa tacaatatct ctcgtccaac aatttcccga tgggtttcag
     1081 aatatcgtcg atacgggaaa aatgcttttg ctggacaagg aaaaagatta cctgataaag
     1141 cagatttcta cattcttgaa caggaaaata aacgactaaa agaggaagac gagtgttcta
     1201 cgaacacaaa gatcgatacg gatctccagg gatcactcaa aaactttacg atgaaggaat
     1261 cgaaatcaat aaacgtgttg tagctgttct tatgcgtgaa atgaatttat gcacaagagg
     1321 atttcatcga cgcaaatctt ctcatggtag agaaaaaaca atcaaagaaa tggtaaaaaa
     1381 aatgttgaat cgataatttg aacagaacca aattgatgct gtttgggtga cggatattac
     1441 ttacattcca tgtaatgacg gtcgcctcta tttgtccacc tatattgact tagcaactcg
     1501 tattctacgt tactatatgg tggattcaca catgaaaaaa gagattgtcg tccgtccatt
     1561 acaaatttat taaggagaat tgcctacggt aattcattcc gatagaggta gccagcatcg
     1621 gtcttttatt tatcaagagc tgttgaccga ccaccacatc acacattcaa tgagccaaac
     1681 tgggactcct gttgacaatg cagtaattga agcgtttcat cgatcaatca aatgagaatt
     1741 aatcgactcc aacaaatata aaagtaaaat agagatgaag gtgttaatcc aagattattt
     1801 agaagactac tacccaaaca aaagaatcca tacaaaattt atgatgactc cttgacaatt
     1861 tgaagcgaag aaaataa
//
LOCUS       DACESX010000224         1836 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.229, whole genome shotgun sequence.
ACCESSION   DACESX010000224 DACESX010000000
VERSION     DACESX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.229"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..1016)
                     /locus_tag="IXF88_002691"
     CDS             complement(<1..1016)
                     /locus_tag="IXF88_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1509380.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIY"
     gene            1710..>1836
                     /locus_tag="IXF88_002692"
     CDS             1710..>1836
                     /locus_tag="IXF88_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1509381.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQ"
BASE COUNT          656 a          293 c          222 g          665 t
ORIGIN      
        1 ttataaatcg taatatgcat ttcattctta aagctttctc ttaatttagt ttcacttaca
       61 gaaaaataat tagctatttc ctttacagta attcttttat agatatttaa gccgatgaat
      121 tgaacgacag aagaaattaa gggagaaaga tgctctatct tagtttttcc gatttcctta
      181 gtataataaa caatcccaga atccctcact tttaagacct caggtaacga tacagctaat
      241 tcgttttctt gtatcaaagc atctctactt tgatacgctt ctattatatc cattcctaat
      301 gttatagacg tttgtgctaa cttttcaaaa acaatgatag aataattttt ttcagaccga
      361 atcgtatctc cactaacact tggaattatt ccattactca gattaaaaac catgttttct
      421 aaatttcgaa tatctccatt tttaacatat tccagtattt tattttcata aagaaacaga
      481 tatatttctg gatcgtaatt ttgtgataac agattatcta ttttgatttg ctcgatagtt
      541 ttacttagat ttcctgtata ttcaattaac ggttcatgga atagatcttc tatttttcca
      601 gtaaaaaaat aatgaactaa aatcagaata tctcgaatgt ctcctaaaga atacaaagtc
      661 aaattttgca atgaattata gagtcgttct ctatcagcca ttgcaacatt caaattgttc
      721 atttttagtt taaaaaaatc tttatcaata ttatttgctc taaatggacc aaataagtaa
      781 tatctggttt taaacatgta tagaagaaac atctcattaa aatcaccagt aaagaaatgg
      841 aaatctttag tttttttaat attatcagtt agtaactttt tataatcata aaaaaaataa
      901 gtagttctat ctgagcgata ctcttttata atactgaatt cggaattaaa aaccacgata
      961 ggtaacttta aacaaacatg aagagacttt aataagactt ctttatctga aaacactttt
     1021 gtttctctct ttcctttcta catcattaaa tagtatttta tacgttatct aaaatactat
     1081 tcctctgtca aaataagcta acttacttca ttataaaata tccacttatc gctttcatgt
     1141 atattccacc gtaaaaagta cattttatca ccgagaactc atcatttttc atcatgatga
     1201 atactattta catatatcta gttcaaagac aataattatt atgatccgtt attatctaag
     1261 cactatacag ataataggaa tttttcgttt ctaaatactc caatttggga atccaataaa
     1321 aattttcgtt caaatacgtg tctattcgaa ctcttttcta gttgttaatt atgaaggggg
     1381 aagaaataaa aaatacaatc tatacacgaa taagaatatt atccaaaagg aggttttaga
     1441 aaagatttat tatacgtaaa aagctcaaga cattttctta gccataagga catgtttgat
     1501 atttaagaag atatatgaat tttcttttct aagtataaag ataactatcc attatcaact
     1561 aaaaaaacat ttttgaaaat aaacatcagc agtcctatca atgcatgtga gcttatagaa
     1621 gttattcgca gcgcagcata attgcattag cttcttttcg tgttttttgc ctcttttccc
     1681 tttaaatctt aatggaggag gtgtgcaata tgtcagataa cgacgaacgc gtaaatgccc
     1741 agttgattcg attctttgaa aaggtcattt tgaacaccgc taatacttat tataaaaaac
     1801 aagaagaaat tcaaaaacat gaacaacttg atcaac
//
LOCUS       DACESX010000225         1835 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.230, whole genome shotgun sequence.
ACCESSION   DACESX010000225 DACESX010000000
VERSION     DACESX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.230"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(279..734)
                     /gene="tnpA"
                     /locus_tag="IXF88_002693"
     CDS             complement(279..734)
                     /gene="tnpA"
                     /locus_tag="IXF88_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1509382.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            977..1835
                     /locus_tag="IXF88_002694"
                     /pseudo
     CDS             977..1835
                     /locus_tag="IXF88_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          581 a          357 c          346 g          551 t
ORIGIN      
        1 agattatttc tgcgccagca ttttctaatg tttgaatttg tgttttgtaa tcttggttgc
       61 ttgtgctaat tcttgaatag ccgtaaattg ccaaaatttc atcacccttt ctggacataa
      121 gtttctgaca ccagttaaaa tcaagtctat cagtaaccca tacacccgtg aggacgggtg
      181 tatttttaat ccccctagaa ggggtcgaca ctcgctgccc cttaaaaggg gatttttaaa
      241 agaccgccaa ctgctcatct tcactcactt acttctcttt attttttaaa tggttctacg
      301 tactctcttt tacttataat atatcgaatg cgatcatcag cttcttgttc acggatgtat
      361 ttttcatttg tcttttgatt caatccgact gtactcacat agtatctctt cgaccaaaag
      421 gatttgtttc cgtatttata tttgagattt gcgtgctgct cgtggattag taccgcacta
      481 cgacctttca gataccccat aaaactggat atacatattt ttggtggtac tcgaacaagc
      541 atgtgaatat gatctagcat tgcatgtgct tctatgattt ccacatcttt catttcgcac
      601 aacttcctaa gtatttttcc aagatctttt cgtaattgcc catagattac ttttcgttga
      661 tattttggtg taaaaataat atgatactta caattccaac gagtatatgc taaactttta
      721 tcgtcatttg acatgaaaaa actcctttcg atctgatgag cggttggtag ccactcacta
      781 gtatatcgaa aggagttttt atcgctaaag atacttcttc cacgcgtaga aacgcgcggt
      841 tttcttgttt cgcacgcttt acgcacaaaa ctgactgaag tcattaagaa aataaaaggg
      901 tctaaaactt ttaagttttg taccgacccc caaaagttag accaaaaaat ctaacttttg
      961 gaggtcggta ttttttatgg ctaagtatac ttttgaactt aaattaaaga tcgttcatga
     1021 ttacttggat ggtaaaggcg gaagtgatta tctggcaaag aaatattcca ttaaggctcc
     1081 atcacaagtg aagcgctgga tcaatgccta tcaagaattt ggtgaagagg gtctagtaag
     1141 aaagcgacaa aagaaaaaat attctgttca atttaagctg gatgcgatag agttatatct
     1201 aacaagtgag ctgtcctatc gtgaagttgc caacacactt aacatgaaca accctaattt
     1261 gatcgctagt tggatgcgtc aattccgtga gggtgggatc gatggactct caaaaacgaa
     1321 aggatgtcca cctatcttgt ctaagaaaaa tgaacccaaa aataagaaaa aatctacaac
     1381 taaagcaacg tctaaagaac gtgaacgcat cgaggagttg gagaaacgag tccgttccct
     1441 tcaaattgaa aatgcctttt taaaagaatt gaggaagctg cgaaaacagg aagcccaaca
     1501 acgacgaatg aagcaatcgc acgaatcatc gcaagcctcc gaggaccgtt taaattagtt
     1561 gagcttctag aaactttaaa gttccctaag gcaatcttta tgtactggca aaaacgattg
     1621 gaccgtaaaa atccagatca agagattgaa gcggaaatgc ttaaagttcg ggagaagcac
     1681 aaggattatg gctgtttacg gatgaccaat gaactaagaa accgcggttt ctctatcaat
     1741 aaaaagaaag ttcagcgcct gatcaagaag ctaggaataa aagtaacgac atacacacgt
     1801 aaatcacggc gctacaattc ttatcgagga caagt
//
LOCUS       DACESX010000226         1800 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.231, whole genome shotgun sequence.
ACCESSION   DACESX010000226 DACESX010000000
VERSION     DACESX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.231"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            37..519
                     /locus_tag="IXF88_002695"
     CDS             37..519
                     /locus_tag="IXF88_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509383.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            716..1606
                     /gene="pstS"
                     /locus_tag="IXF88_002696"
     CDS             716..1606
                     /gene="pstS"
                     /locus_tag="IXF88_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1509384.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          596 a          308 c          377 g          519 t
ORIGIN      
        1 gtatgatgag agaaaaagta gtttggggga acgttaatga gaagaaaagc agtaatcgat
       61 cggctttatt tatttttttg gttttttcct atcgttttgc taccttttac atgggtaaat
      121 gctgttgtac aaggtgcgga gatattaggc ctgatccaag gaagcggatt tgtggtttct
      181 aggaattttc cagctttgat tgttttctat ttagtgataa gtgtaggtct cttattacca
      241 aaatctctga tcaatcacta caattggcag ctgctttact tttcttgctt attagtattt
      301 acatctatgt tttctttgct gcctcgcttg attatcggcc ctaatggttt gagcgagagt
      361 ggattgaagg atttagctta ttcctccatg tatttcttga cgatcaaacc atggttctat
      421 ttagtaattg cctcatttat tcttgcaaca tttttttact tctggacaga aatcaggata
      481 aaaagtgaac gatctgtatc tataaaaaat aactattgat ggtggatcat cagtaaaaaa
      541 acatatgagc tgcgtaaaat gcatgtttct catatgcttt ttttattatt atgtaaagaa
      601 tgcgaaaaac gtttacatga tttttacata acttaactat atttttacag caaccatacg
      661 agggatttac agtcgactga taaactttag ttgtaaataa gaaaggagca ataaaatgaa
      721 aaaagtattg tttactctag gtatgatggg aattctttta gccggatgcg gctcaggatc
      781 aggaaataca gatggctctg ccacacaaaa taattcaagt gactcaaatg aacaagtaaa
      841 aatcgttgcg gtaggttcga cagcattaca gccgttagtc gatgcagcac aagaaagctt
      901 tgtacaagaa aatccaaatt atcaaatctc tgtacaagga ggaggaagcg gaactggttt
      961 gagccaagtc gaagctggag cagttactat cggaaactct gatgtatttg cagaagaaag
     1021 agacggagtt gatgcttcaa aattagtgga tcataaagta gcagtggtcg ggatggcacc
     1081 aatcgtcaat aaagatacag atgtcaaaga tatcaccaaa caggaattga ttgatatctt
     1141 tacaggaaaa atcactaact ggaaggaagt tggcgggaaa gatcaaaaaa tcaacgtcgt
     1201 gaaccgtgca aacggcagcg gtacccgcgc gacattcgaa aaatggggac tggatggtgc
     1261 tacccctgtg cagtcccaag aacaagattc atcaggaaca gttcggcagc tggtaagtca
     1321 aacaccagga gcaatcagct atttagcttt ctcatatctc gatgattcca cacaagcact
     1381 aagtatcgac ggtgtagaac caaaagaaga gaatgtagca gataacagct ggggaatctg
     1441 gtcatatgaa catatgtaca cgaacggaaa accttcccct gaagtccaaa aattcttaga
     1501 ctacatgatg acagaagaaa tccaagaagg acctgtcaaa gaattgggct atttaccaat
     1561 cacaatgatg gaagtagaac gtgatcacga aggaaatatc aaataactag cagataaaca
     1621 tgccacggta gtcgttacta ttaaggaaaa atcaactatg tttccccaca tatacatttt
     1681 tcctttaatt gtgaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg
     1741 tcagctgatt gaacaccgtt tattcgaaaa aaagagctac gacatagaac tgtcgtaact
//
LOCUS       DACESX010000227         1774 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.232, whole genome shotgun sequence.
ACCESSION   DACESX010000227 DACESX010000000
VERSION     DACESX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.232"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            70..582
                     /locus_tag="IXF88_002697"
     CDS             70..582
                     /locus_tag="IXF88_002697"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1509385.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            579..1616
                     /locus_tag="IXF88_002698"
     CDS             579..1616
                     /locus_tag="IXF88_002698"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1509386.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          631 a          339 c          378 g          426 t
ORIGIN      
        1 cccctatgtt aggctagttg tcgtatagga taataagtta taataaagac tatacggagg
       61 atgattacca tgacaaataa aaacaatgaa cgttactcta ccgaactgaa agcgacagcc
      121 atcaagaaaa tgatgccacc cgaaaatagg gggattcgcc aattaagtca aactttgggc
      181 attccagaag cgactcttta ttcttggcgt aaaaaagcta gaattcaagg gaatactaca
      241 cccggaacga aaaaaaatgt cgaaaaatgg tcaagtgaag ataaattttt aattgtgatg
      301 gaatcattct caatgaacca agtagaactt gcggaatatt gccgaaagaa gggcttattt
      361 aaagaacaaa ttgaggcttg gaagaaaacg tgcttatctg cgaatgaaca ggaagaaaat
      421 cgaacgagag aacttgcaac tgaactaaaa gaagagaaaa aacaagcgcg tcaattagag
      481 aaagaattac gtcataagga aaaagcatta gcggaagccg ctgctctctt attactaaga
      541 aaaaaagcac acgcgatttg gggggaccaa gaggaagaat gatttcatct tcagatcgca
      601 aacaagctgt tgaacttatt caagaagcca atcgcaatgg ggcccgtctc acctatgcct
      661 gtaatgagct gaatattagc gttcgaacct acgaacgatg gacgcgtgaa ggtacgatag
      721 cgcatgatca acgccctttg gcgaaacgtc ctgtacccaa aaataaactg acggatcaag
      781 aacgcgaaaa aataatagaa accgtttcaa aaaaagaatt tatgaatttg ccacctagcc
      841 aaattgtacc aaaacttgcg gattgctcga tttatattgc atcagagtct agcttctatc
      901 gtgtattgcg agaaaaaaat atgcaacatc accgtggccg aagtcaagtt tcacaaaaac
      961 gcataccgcc aagtcacctt gcgacgaagc caaatgaagt ttggacttgg gatattactt
     1021 ggctaaaagg tccaataaaa ggactctttt accggttata tctcatcatt gatttattta
     1081 gccgtaaaat ggtaggctgg gagatttggg aatcagaaga agctcgtcat gccgaaaaac
     1141 ttattaaaca gacggtgctt aaggaacgta tccaaggaca gccgttggtt ttacactccg
     1201 ataatggcag ccctatgaaa gccgcgactt ttcaagtctt actcgaaaaa ttaggaattc
     1261 aaagttctta ttcaagacca cgagtgagta atgacaatcc ttattcggaa gcggctttcc
     1321 gaacgttaaa atatcgacca gaatatccca ataacggctt taaaacaata gatgatgctc
     1381 gaaaatggac gaaagagttt gtcgattggt ataacgattc tcatttgcat agtgggattc
     1441 aatttgtgtc tcctgcagcg tgtcatgaag gacgacatct cgctatttta gagaaacgaa
     1501 agcaggttta cgaggacgct aaaaagcgcc atcctgaacg ctggtcaaaa ggtgttcgaa
     1561 attggactcc acatcaatcg gtggcgttga atcctttaaa agaaacagaa agttaacagg
     1621 agcataatct ttcaagatta tcaaataacc ctcgtcaccc aataggggaa aaagttattc
     1681 ttcccctatt ggataacgag gccaaccgac catagggagg ttctgctttt tgataggtgg
     1741 aaaataaaaa tacgacaact atattgacaa acac
//
LOCUS       DACESX010000228         1774 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.233, whole genome shotgun sequence.
ACCESSION   DACESX010000228 DACESX010000000
VERSION     DACESX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.233"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..1053
                     /locus_tag="IXF88_002699"
     CDS             <1..1053
                     /locus_tag="IXF88_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAQ1509387.1"
                     /translation="VTGANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTK
                     QSGSIKDYKGTPRTVLVQRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGP
                     LGVRKMTLIDNDCFQAENLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVE
                     VESMNVLDLISMQPGFFKEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIG
                     GHTLFINYSLNQKGCFNCLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNA
                     IASTECAINAIKLFHKVNQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFE
                     ERFFSKYCKVCGELDD"
     gene            1046..1507
                     /locus_tag="IXF88_002700"
     CDS             1046..1507
                     /locus_tag="IXF88_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509388.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
BASE COUNT          623 a          229 c          342 g          580 t
ORIGIN      
        1 gtaacaggtg caaataaaag atttttttta aaacttgaaa aaagaaagaa tagacataat
       61 gttttactta ttctagatat cccaattgaa gaaaagtcaa ataatgtcat aattggattt
      121 ctttttacaa aacaatctgg atctataaaa gattataagg gtacgcctag gactgtatta
      181 gttcagcgtc atgatgaatt atatttggtg aatagaacga gcggagaaaa ctatggattg
      241 ggtgctaata tagctattgt tggggtaggg tcagttggca gtaaaattgc tgaaaatctt
      301 ggtccattag gtgtgagaaa gatgacactc atagacaatg attgttttca agctgaaaat
      361 ttgtatcgaa attcacttgg tgcagatagt ttaaaaaatg cccgatctca gaataatttt
      421 tttaaagttg atttaatgca ggaggagctc gaaaaaaaat tcccatacct ttgtgttgaa
      481 gtggagtcaa tgaacgtttt agacttaatt agtatgcaac ctggtttttt caaggaatat
      541 gactatgttt ttgtttgcat aggagataca atggcaagcc tggctttaaa taattatttt
      601 tccaaaagta gtcaaaaagt tttctattct tggctggagc catatggcat tgggggacac
      661 actctattta taaattattc tctaaatcaa aaaggttgtt tcaattgtct ttatacagat
      721 tcaaactcta aaaataggtg ttcaaataga gcttctctgg taaaagaagg acaaattttt
      781 gaaaggactt tagcgagttg ccgctcaaaa tttataccat ataatgctat tgcctcgacg
      841 gaatgtgcaa ttaatgcaat aaaacttttc cataaagtca atcaaaataa tatgaccaat
      901 agtactcttt tttcatggat aggagatggg agtacttttg agaatttagg atatcaatat
      961 agcgacagaa atacttttct taatggagtc aataagtttg aagaaagatt cttttctaaa
     1021 tattgcaaag tatgtgggga gttagatgat taaattttcg tgtgaccgta ttgtcattga
     1081 agtaagggat gaagttctta agatatttga acaatatcgg cagggaaagg atgacttgcc
     1141 tgaagctgga ggagttcttc atggaaaaag acttaagaat gggagcataa taattaccga
     1201 tataacaaca cctcaaatag gagatataca aaaaagatta ctctttaaaa aaaaccgaga
     1261 aattcatcaa cagctttcta atgagaaatg gatagaatca aataagtttt ctattgcatt
     1321 gggagaatgg catactcatc ctgaagctgt accaactcca tcaaatgttg ataagaaaag
     1381 ctggagactg aacgtgagca agcaacatga tgatagagtt tatgttttta ttatcgttgg
     1441 attttctgat ttgagaatgt ggttactgtc aaaaaaatca gatattaggc aggctataaa
     1501 actataaagg agtgattagt tgaaatcaca aaagaatatt tggtggtggg ggtttttagt
     1561 aggaaaggta atgataccag cccttctctt aatttctcta ccgatgatta cttttttctt
     1621 taccaataaa aaaatgagta aagaagccgt gatttttttc tttggagacc aaaaagctac
     1681 ctttattgaa acgttagtaa catctcttca gcttaatttg aattacattt tttcaattat
     1741 tattgttata tcgttattga atttttttaa aaaa
//
LOCUS       DACESX010000229         1770 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.234, whole genome shotgun sequence.
ACCESSION   DACESX010000229 DACESX010000000
VERSION     DACESX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.234"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..1084
                     /locus_tag="IXF88_002701"
     CDS             <1..1084
                     /locus_tag="IXF88_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1509389.1"
                     /translation="HISGTQAGFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLL
                     FSIASLLMYFASNIYLVYLFRFIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIG
                     AQVGAALAAILAAVIIPTFGWKMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKT
                     KLSRLFESKHLAWQTCGLSIMVTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVST
                     ILGMSLGMMTFGIIMDKLGPKLAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFI
                     NGMYGGYGAVISSLYPTEIRATANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFL
                     SGIYLVSLLVLTTLTGVTALRESLQH"
     gene            complement(1250..1672)
                     /locus_tag="IXF88_002702"
     CDS             complement(1250..1672)
                     /locus_tag="IXF88_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1509390.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT          461 a          312 c          373 g          624 t
ORIGIN      
        1 tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat
       61 tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca
      121 aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt
      181 ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat
      241 gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg
      301 tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac
      361 gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat
      421 tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac
      481 caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat
      541 catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc cttcaattat
      601 gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt
      661 aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact
      721 agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg tgttagccca
      781 tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta
      841 tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa
      901 taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt
      961 cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt
     1021 cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca
     1081 ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta
     1141 ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt
     1201 gatgcagggc agctttttca actgctaatt tccttctatc tggcggctac tattctttga
     1261 cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac
     1321 tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt
     1381 caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg tagattggga
     1441 agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat
     1501 caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg
     1561 ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta
     1621 aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc atattttctc
     1681 ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa atatcatatt
     1741 gtcacaccgt caatagtgca taaacctgtt
//
LOCUS       DACESX010000230         1694 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.235, whole genome shotgun sequence.
ACCESSION   DACESX010000230 DACESX010000000
VERSION     DACESX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.235"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            138..914
                     /locus_tag="IXF88_002703"
     CDS             138..914
                     /locus_tag="IXF88_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1509391.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            929..1132
                     /locus_tag="IXF88_002704"
     CDS             929..1132
                     /locus_tag="IXF88_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1509392.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1148..1486
                     /locus_tag="IXF88_002705"
     CDS             1148..1486
                     /locus_tag="IXF88_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1509393.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            1473..1652
                     /locus_tag="IXF88_002706"
     CDS             1473..1652
                     /locus_tag="IXF88_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509394.1"
                     /translation="MGKFNRALVFSAPLIIYALELWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
BASE COUNT          653 a          247 c          335 g          459 t
ORIGIN      
        1 agaaagcttt aaaatcaatg tttttatgtc ttatttaatt atgctcttag tatagagtat
       61 tactcagata ctgtcaattg aatttagaaa aaagttgtta aatttgttta cgaatataaa
      121 agaaaggaat gagaaatatg aacacaccgc aaatttttaa tttcgaacaa aacgaagttc
      181 ggacaatttt agtaaatgat gaaccatatt ttgtaggcaa agacgttgcg agtgttttgg
      241 gttattcaaa cactaaagat gctttgtcgc ggcatgtaga tttggaagat aagatggggt
      301 cgcgaatcac gacctcaggt caatcaagag agatgacaat catcaacgaa tctggtttgt
      361 acagtttaat cttaaaatca aaacttccct ctgccaaaaa atttaaacgt tgggtaacaa
      421 gtgaagtgtt gccagcaatt agaaaacatg gaggttatct aactccagaa aaagtagaag
      481 aagctttgct taatccagat acaatcattc aattagcaac tcaactaaaa gaagaaagaa
      541 ctggaagatt aatcgcagaa caaaagattg cagagtacga acctaaaatc tcctatttag
      601 atagcatatt atcttctaca gattcagtaa caattagtca gattgcagca gattatggga
      661 tgtctccaca acagatgaat aaattacttc ataaactagg tattcagaaa aaagtcggta
      721 accaatggtt attgtgcaaa aaacacatga accaaggata cacaaaatct catacaactg
      781 agatcccgaa agccgatggt ggcactaaaa ttgtaatgaa taccaaatgg acacagaaag
      841 ggcgtctatt tatctacgaa ttactaaaaa aagaaggata ttaccctcaa atggatttag
      901 aggaaattgg ttagaaagga gttttagtat gactgacatt gcagaaatca ctcaacgaga
      961 tagagaaaaa atcaaagaat atgtcgaaag ttcaaagttc ttaacttaca ccatgcttgc
     1021 tgaaagattt ggaattagca aaagctactt atctttaatt ttaaacggta aaaagacttc
     1081 tgcagaagca aacagaatta tagattcgat tatcactatg tacgaattgt aaagggagga
     1141 acagctaatg caatatctag aagcaaaaat cccaattcca gaaggctatg taattatctc
     1201 ccaagtggat tatgaggagt taaaaaaagc tgatgatact ggtagatgga tgacgttgcc
     1261 agaagtgcta gaacggatta acagaaaata tgattggttt acttctagag ttttaaagaa
     1321 cccaagatat agaaatatta tcgatatcga gaaaaacaaa aatggatttg tctattatcc
     1381 agttgaagga agagacacat atctattttt aagaagtaaa acacttgaat ttttagaaac
     1441 aaatttttcg gaaatcttaa ggaggcaagc ggatgggaaa atttaataga gcgctagtgt
     1501 tcagcgcacc gctaatcatc tacgctttag aactttgggg aagcaggcaa gcgttgatag
     1561 gaacgattgt ttacatggtt tggattttta tggggcttga tgaagctgaa gctgagtaca
     1621 gagcgaaaaa gccaaccgag ggggctgact aaaaatgaat agaaaagaga aactagaatg
     1681 tatattacta ttac
//
LOCUS       DACESX010000231         1691 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.236, whole genome shotgun sequence.
ACCESSION   DACESX010000231 DACESX010000000
VERSION     DACESX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.236"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            468..1640
                     /locus_tag="IXF88_002707"
     CDS             468..1640
                     /locus_tag="IXF88_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ1509395.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          599 a          320 c          296 g          476 t
ORIGIN      
        1 aaaaagttca aaagatcaag tgtgcgcttc gctcccactc ccctcgacgt tttagtagcc
       61 tttccctcac ttcgttcagt ccaagccaac taaaaagttt tcgggataca ttctccttct
      121 cttcctaata atcaattaaa attttaatct ttatatttct gctaatcact caggaaacag
      181 tacagaaaaa caaacacgta tcatagatat aaatgtaatg gcatagtgcg ggttttattt
      241 tcagcctgta tcatagctaa acaaatcgag ttgtgtgtcc gtttttgggc gttctgctag
      301 cttgtttaaa gtctcttgaa tgaatgtatg ttctaagtca aaaaactctg acagcgcctt
      361 tatatagata aagtccgtat aattgtgtaa aagtaaaaag gccatataac agtcctttta
      421 cggtacaatg tttttaacga caaaaacata cccaggagga cttttacatg acccaagtac
      481 attttacact gaaaagcgaa gagattcaaa gcattattga atattctgta aaggatgacg
      541 tttctaaaaa tattttaaca acggtattta atcaactaat ggaaaatcaa cgaacagaat
      601 atattcaagc aaaagaatat gaacgaacag aaaaccgaca aagtcaacga aatggctatt
      661 atgagcgcag ctttacgaca cgtgtaggca cgctagaatt aaaagtaccc agaacacgtg
      721 atggccattt ttcacccaca gtgtttgaac gttatcaacg aaacgaaaaa gccctcatgg
      781 cttcaatgtt ggaaatgtat gtatcaggcg tttcaactcg taaagtatca aaaattgtgg
      841 aagaactttg tggtaaatcc gtctctaagt ccttcgtttc tagcttaaca gaacagctag
      901 aacctatggt taacgagtgg cagaatcgtt tattatcaga aaaaaattat ccttacttaa
      961 tgaccgatgt actctatata aaagtacgag aagaaaatcg agtactctca aaaagctgtc
     1021 atatagcgat tggaataacc aaagatggcg accgtgaaat tatcggcttc atgattcaaa
     1081 gtggcgaaag cgaagagacc tggacaacat tttttgaata cctaaaagaa cgcggtttac
     1141 aaggtacgga actcgttatt tctgatgcgc acaaaggatt agtctctgcc attagaaaat
     1201 ccttcaccaa cgtaagttgg caaagatgcc aagttcactt cctaagaaat atctttacca
     1261 ccattcctaa aaaaaattca aaatctttca gagaagctgt taaaggaatt tttaagttca
     1321 cagatattaa cttagcgcgt gaggctaaaa atcgattgat tcatgattat atcgatcaac
     1381 caaaatattc aaaagcttgc gcatcattgg atgatggatt cgaagacgcc tttcaatata
     1441 ccgtacaagg aaattcccac aatcgactaa agagtaccaa tctaattgaa cgactgaatc
     1501 aagaagtacg cagaagagaa aagattattc gcatcttccc caatcaaaca tcagccaatc
     1561 gcttaattgg agccgttctt atggacctac atgatgaatg gatttattct tcaagaaaat
     1621 acatcaattt tgataagtag aaatggtaaa aacattgtat agcattttac acaggagtct
     1681 ggacttgact c
//
LOCUS       DACESX010000232         1683 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.237, whole genome shotgun sequence.
ACCESSION   DACESX010000232 DACESX010000000
VERSION     DACESX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.237"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            78..371
                     /locus_tag="IXF88_002708"
     CDS             78..371
                     /locus_tag="IXF88_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509396.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            364..660
                     /locus_tag="IXF88_002709"
     CDS             364..660
                     /locus_tag="IXF88_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1509397.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            735..>1683
                     /locus_tag="IXF88_002710"
     CDS             735..>1683
                     /locus_tag="IXF88_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1509398.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDG"
BASE COUNT          644 a          231 c          335 g          473 t
ORIGIN      
        1 atacatgtta tatgaattat caatgtaccc agtaatatgc tattttcatt atgtaaaaaa
       61 taacaggggg tgtgtagatg gagattgatg aaacttgttt gagtaactct tataaaagta
      121 tgaatgactt ttttgaggaa atggaactgg attcagaatt gctctatcaa ttttatcttg
      181 cttttcgagg gcaacagatt tcttttccaa tgaaaatgta cgatcgtgag ttagttagaa
      241 aaagaatcga aaacatgata gaacaggaaa aaactgtaga tatccgtcaa ttgacagaag
      301 tttatggttt ctctgcaaga tggattcaaa aagtaattaa acaaaaagaa aggagacgag
      361 tacatggcta agctgattgt attaggtggt ttaatttacg gtggatattg gttatataaa
      421 tatatgcttg cgcatccgaa tatagataaa ggaaaagtaa ttaagacagt tggggtaatc
      481 atagttggac tcatagctct aactaagaaa aataatacta atagcccaaa aataatgaga
      541 acttatcgct gtagaggatg tggaagggaa atttctacta gttttaatcc atctgagagt
      601 cctcaaggaa gagaccgctg ccatacgggc tatcgacatt attgggaaag aatcgattaa
      661 aagattacca tttagatcaa ttaattgtct gatagaataa gtacgtgagg gagaaattga
      721 aagggggaaa tataatgaaa aaatggctgt tattactaat aggaatggga attttattca
      781 ctggttgtac atctgagaat acgaggacta aagcgactat agaaactgat acaacaattg
      841 agactaaaaa aagtaaaacg caatcaactg aacaaactac aacagaaatg aagcaatctg
      901 tggaaacaag tgcaagttca atcactgaat cttcttctga agaggttaag aatactttgt
      961 gggatacgaa taaagcttcc aaattagaaa cttttgtcac acaatgggga aagacattag
     1021 gtcaagaata taaaagctac aatcttcaga ataatgtaag tctgtatggc actcctttac
     1081 cacaagccgt gattaatggc gattggaaaa tggcaatcaa tgaagcacca gttaccgtac
     1141 aatggtcaga agatggaact ggtcatgcag atttcaattt agtagcagtt tattctgatg
     1201 tagaaactgg agaatattta gggcagcatg tttacttttt cggttttcaa aatggacaac
     1261 ctaaagttta tttaacgcaa caaaatcaag gtaatgaaaa taattatctg tactttaacg
     1321 aaactcaaaa tcaacagtta aaacaaggtt tcagtgatat tgtgaatgga caaactcctc
     1381 aaacaccagt agtagaagaa tctactcaac aagcaacaac tacaacgaca caatctacaa
     1441 gtagttcagg aatttcaagt tgggaagctg ctaaagaatc tgtgctaaat aataaagctt
     1501 tatggttgta tgattctcct gataaaaatg tatcaaatac taatttaatt agcacaaatg
     1561 aatatatgaa agatctaatg caagatgaaa atggaagcta ctattcaatt aatattgtaa
     1621 cggatgatga tggaaacacg accatggctg gtggaaaaca atttcgtgtt tatacagatg
     1681 gaa
//
LOCUS       DACESX010000233         1664 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.238, whole genome shotgun sequence.
ACCESSION   DACESX010000233 DACESX010000000
VERSION     DACESX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.238"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            137..1615
                     /locus_tag="IXF88_002711"
     CDS             137..1615
                     /locus_tag="IXF88_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1509399.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          610 a          228 c          301 g          525 t
ORIGIN      
        1 tcaaaaaaaa gataaaaaat gagaataact gatgaaaaaa caagcataat ccatcataaa
       61 atcagtatag gtgaaaagat gaactttcat ctgcatatca agtaattggt atgaggcatt
      121 ttttgaggtg taggatatga agttggatta ccttatagaa gaggaagata gagaaaaact
      181 gctgctagct agatatttag aatttgagaa aaagaattat gtagtgctgc ctaagttata
      241 tgaaacatta ggtttgtcta aatttaaaac aagaaattat atcatagaat taaataaaga
      301 atttactgaa ttcaaaacaa agccagagat ccaaatttca gaagaagcag agatccatgt
      361 cgaacgtctg aatttgacgg atatcaaaaa tttaagaaga atctactttc aacgatcaaa
      421 aattgctcga ctgttaacgg agatcgtgga ttcaaaagta acgatcgaaa aatttgcaga
      481 gaatcatttt ttaagcatat caagagccta tgtaaaaaga agagaattag tctcttttct
      541 tgaacagcat caaataaagt taaagaaaaa catattagtt ggggaagaaa taaacatacg
      601 gaacgtatta tttggggtat atttcggggt ctttaatggt ttttctatgc catttagaga
      661 aagcgtcatg aatgaagcaa agagtatcgt gcaatatttt gaatatctgt tcaatttaaa
      721 actgtctgca acaaaacgaa tcaaattcaa tttgctaacc actattagtt tgttgagggc
      781 ctatgggaat caaccaatcg gaaaacgttt cttttcagat gaatacttta agagctcaga
      841 aggaatgaag atcatcaagg atttatcaca tatttctttt cttcatatcc ctaaagagca
      901 gatcattgat gagatttctt atatcatgtt gtttttgatc acggaagaag aatttgaatt
      961 agatggcgca tttggacttg cggaagctca ttttcatgat atccacatga ttagtcaaaa
     1021 aatgtcagtg gaattgattg atcaattacc attttcgatt gaaatctctg aagaagaaag
     1081 actatatata cgaaaagaac ttcgtatggg tatgatgcat gtcaatcgaa aaaattggat
     1141 attcgacttt gagatgacta gctttacgtc taaaaagcag ctacagtttt tcttcgagac
     1201 gtatccaata ttcagtacag caatccaaaa ggtcatacaa gagaataaac gctatttctt
     1261 gggaaaagat gaacgtttca tgattcgctt attttatgat tatctctttt tattggtcgg
     1321 tatttgtccg gtatcatatt ttgaagcacc gattcatgtc tgtattgatt tttcgcaagg
     1381 atctgcttat accgattaca ttatttctca agtgaaagga tttaaaaatt acaatattgt
     1441 gattgagagt cgaatcactt ctcgtacgaa tatttattta tctgattgtt tacttgaaaa
     1501 atttaataaa gaacagataa tttggaaaaa tccaccgact ccagatgact gggaatattt
     1561 tggaaatacc atcatcagag aaaaaaatag acatttaact gaaaaaatca gctagtattt
     1621 atggatggca gctttgtctc acatatttat tagctgattt atgt
//
LOCUS       DACESX010000234         1583 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.239, whole genome shotgun sequence.
ACCESSION   DACESX010000234 DACESX010000000
VERSION     DACESX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.239"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            34..240
                     /locus_tag="IXF88_002712"
     CDS             34..240
                     /locus_tag="IXF88_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509400.1"
                     /translation="MKQKVIGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYT
                     HVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(602..841)
                     /locus_tag="IXF88_002713"
     CDS             complement(602..841)
                     /locus_tag="IXF88_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509401.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1127..1441
                     /locus_tag="IXF88_002714"
     CDS             1127..1441
                     /locus_tag="IXF88_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509402.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          540 a          216 c          279 g          548 t
ORIGIN      
        1 ttaggaaaaa aatatatttg ccaacctatt ggcttgaaac aaaaagtgat tggagaagta
       61 atcagtaaat tagaaaattg tgttgtgatc tgcgtagacg aatatcatga cgtcgatcgc
      121 aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat atactcacgt ttatgagttg
      181 gaaacaacag aatatctata taactatagc aacaaatatg aacatacttt agtttcataa
      241 agatttaaat aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc actttattta
      301 gttggcagtg attgttcatg atttttgatt tattcaaaag gttcgcctag agaatctgtc
      361 tctgtaacga ggcctggtgg agaaactcga taaaaaccta tactgccaga actgtcctat
      421 tttcggatag acaaactagt agttttaaaa agatcatccg atccaatcat ttcaaaaaat
      481 gcaaaactga tatcaggatc atttagctca ggcttgttat cgtaaagcat ttgtaaaggt
      541 tgcatttagt taggagtaaa cgcgaaacta tcatcattat ttgctgaaga agatgaagaa
      601 gctaattcag agtttgtttg aaataaagga gacgtgctga cagtttcttt accctgtttt
      661 ttgcagaggt agaaactttt atatcggaag tttctaccgg agaatttttc tcatttgtag
      721 aaattgttga atcgatacta ttcgaaattt gcgtatctga aggtatgttc tttgatgcag
      781 aagtcccaca agcagtaagt aacaataaat ttgataaact gataaacaga attcttttca
      841 ttatgtacac actccttata gtatcgttta gcatacacct atgctcagta ttaggcaaaa
      901 tcaatgcaaa actatcaagt cttttatagt atctattagt cttagtaata catatatgga
      961 gagggtgata tcccagttca aaccagatac attggttacc atctgtatta acgattatgc
     1021 ctctaaacgc tttttttatg aaaaaattta taagtgaaaa cgtaggtatg atattatcta
     1081 gaggtaattt ttttgggtat ttaaatagtt gctaggagga tagcttttgg atttttacag
     1141 taattttatc ttaataatag caatattatt acttctaaat atttggtttt ttgataagag
     1201 tagaaatgca gggataggtt ttagaaccaa acgaagtacg tcttctgaaa aaaagtgggt
     1261 atattcacag actatttttt acggaggggt tatttcaatc agtttacttt cttcaacact
     1321 ttattctttt aatgttatag acgtttccat gtcaaatttt atatctatta ttggaatcct
     1381 tatttctgca attattacac aactacttct tgtgtttgaa gagaagtcaa aaaataatta
     1441 aagcaattat gccttttagt acaggaatta ttacttctta caaatgtata gcgaatgtga
     1501 agcctgctgt tttaattgtt ctagaaaaac ttttgagtga acaattattt tatgttagga
     1561 tgaattaatt tctctagagt tta
//
LOCUS       DACESX010000235         1519 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.240, whole genome shotgun sequence.
ACCESSION   DACESX010000235 DACESX010000000
VERSION     DACESX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.240"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            51..449
                     /locus_tag="IXF88_002715"
     CDS             51..449
                     /locus_tag="IXF88_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1509403.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            433..1127
                     /locus_tag="IXF88_002716"
                     /pseudo
     CDS             433..1127
                     /locus_tag="IXF88_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          577 a          217 c          305 g          420 t
ORIGIN      
        1 ttttgaataa aaactattag acaaatgata ttttacgagg aggaacagag atgacgatct
       61 ttgaaacggt tgtaacagat gttggtatag aagcagaagt attcaggaaa gaaaaaataa
      121 tgattttatt tggagatgaa gcaccggaaa cattaacaga ttattgttat aagattgaat
      181 tgaatcaggt aagaaaagaa ataacttgtg atatgaagct ctgttttgat aaacaaatgt
      241 atgtaatcac agcagtagga gatgttgtcc aaactaattt gaatgaatta ggacatatca
      301 cgataaaatt tgatggatct catgagccgg aacttccagg aacaatgtat gtagaagata
      361 aggaaatgcc gcatttaaga aaggggacaa tcattaaaat cgatcaagag gatcagaatg
      421 ggggaaagct atatggaaat catgcttgat acagcaaata caaaaagtat ttattcttgt
      481 caacagtttt tgaatttaac aggcgtaaca acaaatccta caattctgaa aaaagaagga
      541 gcgtcaaact tcttctcgca tatgaaagaa atcaaaaaaa taatcggaca gattcctatc
      601 catattcaga caataggaag aacggaagaa gaaatgatag aggatgctcg agttatcata
      661 gaagaactgg gaaaagatac gtttatcaag gtgccggtga atcaagcagg ccttcaagcg
      721 atcaaaaagt tgaaaaaaga aggttatttg attaccggta cagctattta cacagaatta
      781 caagggtatt tagctattaa taatggagca gattatattg caccttatta taacaggatg
      841 caaaatcaag gcattgatgc tatgaaagtg attgattcgt tgcggactga gatcgaacgt
      901 acgcaaagac caacaaaaat attagcagct agtttcaaaa attgtgaaca agtcactaca
      961 gccattcgat ctggagctca gcgataacgt taggtaaaga gctactagat caagtattag
     1021 agatgccctc gattgataaa gcagtgaggg gcttccaaga agattgggtg caaactttcc
     1081 aagcagaaac gattggtgaa ctaaaaaagg tcgctgatta cttctgaatc aatttctcca
     1141 taaaataaac gatagccttc tcgaaaactc gaggaggcta tctgacgatt caatattcat
     1201 taatttaaag tttgggtgac tattactaac tattacttat ttctcattta aacgatgtat
     1261 tccctaacct ctaatacatc cttgtaactc tgaagttacg ccagttttga ataaatttca
     1321 taaaaacaaa tgaaaagtgt cgcataaaaa tatctttcgt gtttctctag tataacagaa
     1381 taagaagaac aagtccagaa gggatatttt tagtcttttt taaaataact tttcagtcga
     1441 gtttgagttt atgatagttg tttttgaagc gatacggcaa gtgaaaaaat agggaagcat
     1501 gaaagaatct acttaatgg
//
LOCUS       DACESX010000236         1518 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.241, whole genome shotgun sequence.
ACCESSION   DACESX010000236 DACESX010000000
VERSION     DACESX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.241"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            1076..1518
                     /locus_tag="IXF88_002717"
                     /pseudo
     CDS             1076..1518
                     /locus_tag="IXF88_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          557 a          255 c          253 g          453 t
ORIGIN      
        1 ctataatttc atccacgata ttttaatccg ttctacaatg catatctgat atgcattgac
       61 tgtctaactc atatgtgaat gatttataaa atatcccaac gtctcatcta aagaaatgtt
      121 gatcgtcagc aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa atcggactgg
      181 tttgcatatc attttcttcg taaatatcaa aatcatgtta actactatca attactaata
      241 aaaactacca ctttaagcgt taagaactgt taagtttagt taagtgagga tagttcacaa
      301 aatccattgt taaacttcac gaaagtggta aaactcaaaa tgctttatat aggattacag
      361 catcgctctt tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa aattgacgat
      421 gacacgttcc ttacggctca tcagatgaaa cagctacaaa aacgaaatga tcaactcgaa
      481 gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg aactattaaa
      541 ggtcaagatt tacctgaaga tcatcctttt taggaaattg aagcttttta tgttgcacaa
      601 tgcatttata atactacaat gatgttttca ccagatattc ttctagggtg cggtgtaatg
      661 aaacagatac acttgcaaaa gaaaattcac cgcgaattta ctaaaattat gaataattat
      721 gttaatcttc cgaaattttt ttccaaaatt aagggatcaa gctggtgtta tagattgttt
      781 aaggttggca aaaaaataac ttctttttaa acaattatag aaaaccgata ctctgttaag
      841 ttggttgaca cggtgtcgat ttttatgact ttcttattct gaaacttcac taacggacag
      901 tacctaaaaa gtctatagta tggacgttta cgagtactat tttatcaaaa atgctgctca
      961 tttcaactta tatgttttag tgaactaccc ccacttagct tggataggct acaattaact
     1021 agacagaaaa attaaggtgt gtagactaga agaaaacata ccaggaggaa tttttatgtc
     1081 taagagaaca cgaagaactt tttcacaaga attcaagcaa caaatcgtca atctttactt
     1141 agctggaaag ccacgtgtag aaatcattcg agaatatgaa ctaacggctt cagcatttga
     1201 caaatgggta aagcaatcta aaacgagtgg ttcattcaaa gaaaaagata atcttacgcc
     1261 tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa tggaaaatga
     1321 tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga tgcgaataag
     1381 catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca gtcctattat
     1441 tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc tgaagaattt
     1501 atccgcagcc gaaaggcc
//
LOCUS       DACESX010000237         1518 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.242, whole genome shotgun sequence.
ACCESSION   DACESX010000237 DACESX010000000
VERSION     DACESX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.242"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(1016..>1518)
                     /locus_tag="IXF88_002718"
     CDS             complement(1016..>1518)
                     /locus_tag="IXF88_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1509404.1"
                     /translation="KEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTPAENLAY
                     QVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKELEEILNR
                     NIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTFRTKIRK
                     WKRLAL"
BASE COUNT          476 a          286 c          211 g          545 t
ORIGIN      
        1 gattaagata acgactattt attttatcat atttttacta taccctatac ttttacttgg
       61 tatatttatg aataatcgca aatttacccc tatactacta gtttgtaagt tagattaata
      121 aaaatactaa agttatttgt tcttttatta aatacaccta caacttataa attgatggta
      181 tttagataac acgaaagaat aaaaataaca gctgatagta aagtttgttg acgattgcta
      241 ctcaatcgaa agtgagttag tgcgtatgaa cttgcttctg aaaacttatg aaatgaaaga
      301 ccttttacat cttgcagaaa tcgttactgg gattaaacta agcaataaaa actaatgttc
      361 ccctcattct ttgaacttct actatttgat ttatttattc tgataaatga cgatttcacc
      421 ttcaggatct tcaaatcccg tatagaattt atttaagatc tcttcagtat cagtaatagg
      481 ataggcgata ggccacatac cctttgatgg ttagaataaa ctgctcaaaa tctgtatcgt
      541 atgttggctt tagcatatta acaaaggcta tctccaaatt ataaaccata agcgttgaat
      601 cgttcttaac aatttcaatt cttcctaacc caaagtcaga agatataatt accggacttg
      661 ccttaccctt catgtgggtt gttgagattc catatttaaa gggaattgtc ggtactatcg
      721 gcggtctgaa aaacatccca aaaataaaaa aatctttccc ctgctagtct cctaagtccc
      781 tttgtagcgg atatggcacc ataatctcat tttatcgaag tgagtcatgg tttatctgtt
      841 ggccaaccaa aattgataca atctaacgat gcaccctcta cggcattcta ggggagtgca
      901 cttgcattca cccaataaaa accggcagtt agcaaagaac ttatgtagtc gtatgttctt
      961 actgttaaca tacaagaggt ctcctttcca caattgctta gcagtaaacg ggaatttaca
     1021 aagctaatct cttccatttt cttatctttg ttctaaaagt cccaaatgga gcaactgtat
     1081 ttacatgaat aaatttatat acttcccata cagccgtttt ggttgcctcg tctgcccatt
     1141 ttcttttgtg tgcagtgaat agatcttcat cactcattga atcaatcatc atgttaatat
     1201 catcaatatt acgattgaga atttcctcta attctttcaa tggtagatgt gcataggtat
     1261 ttgtaaacca cttatataac tctcctaact gattccattt aaattgatca gaaggcgttt
     1321 taacagataa tccatttctt tcatcatttt cccattttag aattaaattc gtccaaccaa
     1381 cttgatatgc taaattttca gcaggtgttc tatccacttc ctcaactctt ttatccttca
     1441 attcttcagg aatagtttca aattctaaaa tatatttagt ataagtccta tttatttctt
     1501 tttttaattc ttctttat
//
LOCUS       DACESX010000238         1498 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.243, whole genome shotgun sequence.
ACCESSION   DACESX010000238 DACESX010000000
VERSION     DACESX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.243"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            102..422
                     /locus_tag="IXF88_002719"
     CDS             102..422
                     /locus_tag="IXF88_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1509405.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            440..871
                     /locus_tag="IXF88_002720"
     CDS             440..871
                     /locus_tag="IXF88_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1509406.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            1001..1462
                     /locus_tag="IXF88_002721"
     CDS             1001..1462
                     /locus_tag="IXF88_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ1509407.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT          591 a          195 c          253 g          459 t
ORIGIN      
        1 tttacaagat atagtatagc tcattactca gaattagtca aattatttct gcgtattttt
       61 ctaaactttt tttgtaaaca tttagaaagg ttgttttatc gatgaatacc tatgaaataa
      121 taaaagactt ggcgaagagg aaaaagatat ctatacggca gttagaaatt aattttggat
      181 attctaacgg ttatctagca aagtggaaaa ataatactcc taattcagat gaattaagtc
      241 ggattgctga ctatttcgat gtctctgtag actaccttct aggtagagaa gaaagagaaa
      301 cccctaaaca tgtggattta tcagaagacg atactgtttt ttcttttgat ggaaaagaaa
      361 tatctaagga gacaatgcgt aaagcgattg caattgctaa agctttagag gaaaatgaat
      421 agttggagtg atggtttgta tgtatttgaa attgaaagaa atgttgagcg aatacaattt
      481 gaaagtagtt tatatggaga tgaaagagcc tggtttctac tatccaaaac ccagaatcat
      541 ttttttgaat gaaaatctat acggtgaaac tgcagaagcc tttcatttat ctcatgaact
      601 tgcacatttc agcgcctctc attttgaatt ttcagttttg tacgatactt ctacaacttt
      661 tcactcaaaa tttgaaacag aagcagataa aatagctatt ctaattttat taaatatcta
      721 tattgagaac gaactgactg atgaatctca gtttaatctc gaaaaattta tggagtatta
      781 ttctattcag aataagctca gatacacttg ttatgctgtc tgccaatgct attttaagaa
      841 aaaatattct tacgcaaggc aatatgtatg aatacatcaa gaattattaa aaaaaccgat
      901 ttcagcaaaa cattaaaaaa gcccgtgctg caacacgggc tctttcctca tatatgagct
      961 tctacaaaaa aaatcatatc acagaaatga ggaagaaaaa atggaaaaag agattttaat
     1021 aaaatctcca ggaaaaacct tgattaaagt aactgatgat tctatttcaa ttattagaaa
     1081 aggatttata aatttagtta atcaaggtat taaaggagaa aaaactatcc cctttaaaaa
     1141 tatatcagcc gtacaattaa aaaaaccagg tatgagtaat ggttatatac aatttacctt
     1201 gctaggcgga aacgaaagtc gcggaggtat attagcggcc acgaaagatg aaaacaccgt
     1261 tatgtttaca aaaaaatatt ggaatgaaat ggaaaattta aaaaaatata tagagaagca
     1321 acaatctata ttagataata attccaaaga aacacaaatt tcaagtgcag atgaaattaa
     1381 aaagtataaa gagttgttag atgaagggat tattaatcaa gaagaattcg attttaagaa
     1441 aaaagaattg cttggtttat aatattcaat atcaaaagag aaagaggatc tattaatg
//
LOCUS       DACESX010000239         1487 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.244, whole genome shotgun sequence.
ACCESSION   DACESX010000239 DACESX010000000
VERSION     DACESX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.244"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..230)
                     /locus_tag="IXF88_002722"
     CDS             complement(<1..230)
                     /locus_tag="IXF88_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509408.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ"
     gene            713..928
                     /locus_tag="IXF88_002723"
     CDS             713..928
                     /locus_tag="IXF88_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1509409.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
     gene            complement(1169..1355)
                     /locus_tag="IXF88_002724"
                     /pseudo
     CDS             complement(1169..1355)
                     /locus_tag="IXF88_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252699.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          439 a          309 c          216 g          523 t
ORIGIN      
        1 atttgctccg tattatctac aaccgcatat tcatctaaag gcttccacaa aacaggcttt
       61 cccggtaatt tctcgacctt tttatcttcc actaatctcg tcaaataatg gctggcattc
      121 tgacgagaga tacctagatt cttcgaaatt ttctcggcag tgacagaaga catattccct
      181 gttagtcgta tcagctcatc aaaaactcga tctttagcgc tctttttcat tttcccctcc
      241 agttcaacgt gtctagaagc ataataaact atcggctaca atatgtctat tctatatttc
      301 tgttccaatt ttactttgaa acaaaaataa tcgagcttaa actgatatta gttataaaac
      361 tatagttaga ttaccaatct tcctctcata caataaactt ctctcacctg catccaacct
      421 acttatgtga aagaataact gccgattatt tatctttttt acagttactt tcgaacataa
      481 aaaaatcacc ttcaaatact agaaaaacta ctatttgatg gtggtttaga acaatttccg
      541 ttgattcagt atcgtctggt cccacatcct ctgtctcatt aatcacaata gcatactcca
      601 aaggataaag ctggttgata tctcaatgcc tttcacacct atcaattttg tcacacttat
      661 cttttcctag gttctattct atataatcta gaaaaacaaa gaaggagaca ccatgaaaaa
      721 attcattatt ccttgtatat ttcttttctt gagtattttg ttattttcaa cttccatcct
      781 agtcggttct tacctcgatt caagcggaat gctgattgaa ccagcgttct tttgtgttcc
      841 gttgggctac ttaacattag gacttgcttt ctttactgcc ttatacagtt tcgtcaaaca
      901 aagattattg aagaaatact cggcttgatt tgcacgtaaa tcctctgttg aagaaaagtt
      961 agttttttca acaacgaacc ttcgagtatt aagaatggat gttgatgtag acatcaaacg
     1021 tctgcataaa cattcatttt attcctccat taccaacaat tattcccaat cgccaaaaga
     1081 agcttttttc ggaattacct tctccatttt ttcacctgct ggttatattg tcttttgtaa
     1141 taccgatagt cctcttcaat cattttcaat atgcgaaaag aaccaatttt aacatggcac
     1201 tcaaatcaca attcctaggg cggccaaaaa gatacggctc gttgatttca agggtttcaa
     1261 cgagttcatt gacaaaccca gcggtgctat cttcttttgg aataagaagt tgtgatatct
     1321 aaagtgagtt gattcatgtt ataatttgta tgcatgattt tgatcctttc aagggttgaa
     1381 tgatttagag tgcgagtatc ctctcgtact cttttatttt acctaaaaag ctccagaaat
     1441 gacgaatgac tcgtcatttc tagagctttt ttcagagaat tatgtca
//
LOCUS       DACESX010000240         1472 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.245, whole genome shotgun sequence.
ACCESSION   DACESX010000240 DACESX010000000
VERSION     DACESX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.245"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            209..808
                     /locus_tag="IXF88_002725"
     CDS             209..808
                     /locus_tag="IXF88_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1509410.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            857..>1472
                     /locus_tag="IXF88_002726"
     CDS             857..>1472
                     /locus_tag="IXF88_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAQ1509411.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYC"
BASE COUNT          499 a          250 c          315 g          408 t
ORIGIN      
        1 aatgttctga gataatcaaa tcttaccgaa aatatcaaaa gtccaatgtg acacaattgg
       61 attttgtcac attcgttaaa atcgctccat ccttagaggc tctaatagga aatcactttt
      121 cttttagcgc cttttttcta tagcttgttt aaatgtgact tattatctgt tataatcgta
      181 ttaagtaaca tttgagaggt gtctaaaaat gaaaagcgtg gaaccgattc gcgacaaaaa
      241 aaagatcgat gccatgaagg ccattttggc ttccggaaaa tatgggcaac gaaatttggt
      301 gcttttttcg atcgggatca atacagccta tcgaatctcc gatttaagac agttaaaatt
      361 aagggatgtg ctagaaattt cccgtgggcg ggtgatcgtc aaagaacgtc tggccatgaa
      421 ggaacaaaaa acggccaaac acaattcggt cttcatttcg aacaaattgc gaaaagtgat
      481 tcttgattat gttcaatccg aatttcctga gcagttgcaa gcacaagact tcagcaagta
      541 tttgtttcca agtcgaaaag gagcagatac gcctttgacc cgacaaagcc tttggcggat
      601 cattcatgaa gcaggaactg cagttgggtt aaaagaaatt ggtccccatt cgatgcgtaa
      661 gacctttggc tattttttat acaagcaagg aaccaaaaca gagatcattc agtcgctgct
      721 aaaccattct tcacaacggg agaccttacg ctatattgga atcacccagg aagacaaaga
      781 tactgcagta aagagtttgg atttatgaca gaataatgcc ctatataata gaaggaaaaa
      841 ttcaagaggt gagaaaatga acgaagaaaa gcagcctaac tttcctgata aatatcactt
      901 atctcgcaaa gagtctgtgt atttattgaa gaaaaacatt gtagaactgg tgtataatgc
      961 ggggaaattt gaagggttaa atacaacgtt gttacaaaca gaagggatta ttaagtataa
     1021 tcgggccaac aacgtggctg tagatgatgt cctgaccgtc gttaacttaa aaagaggctt
     1081 tgaaatgctt ttaaatgacg tacaggagcc cctgttagag acaagtaagc ggattaatcg
     1141 gatcgttgca gcagaggatg cgctttttcc tggtgaaata cgaaccggtg gggtagaatt
     1201 atcgacgatt caaggaagat atgtgccacc aatgttaact gaagacgagg taaacaatca
     1261 atatggtgag ataatgaatc aagagatatc agatacggaa aaagccctgc gactgtttct
     1321 attcatttcg aaaaatcaaa tattctggaa tggtaatgtg ggtacagcct tgttgattgc
     1381 gaataagatt atgtttagca acggtttagg actattatca atacccgaaa atgtatttat
     1441 gaaatttaac gaacttttat caaagtattg ca
//
LOCUS       DACESX010000241         1470 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.246, whole genome shotgun sequence.
ACCESSION   DACESX010000241 DACESX010000000
VERSION     DACESX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.246"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            131..505
                     /locus_tag="IXF88_002727"
     CDS             131..505
                     /locus_tag="IXF88_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1509412.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IXF88_002728"
     CDS             complement(619..1248)
                     /locus_tag="IXF88_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1509413.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IXF88_002729"
     tRNA            complement(1382..1454)
                     /locus_tag="IXF88_002729"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          495 a          225 c          258 g          492 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa
//
LOCUS       DACESX010000242         1469 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.247, whole genome shotgun sequence.
ACCESSION   DACESX010000242 DACESX010000000
VERSION     DACESX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.247"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(50..122)
                     /locus_tag="IXF88_002730"
     tRNA            complement(50..122)
                     /locus_tag="IXF88_002730"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(87..89),aa:Gln,seq:ctg)
     gene            complement(168..596)
                     /locus_tag="IXF88_002731"
     CDS             complement(168..596)
                     /locus_tag="IXF88_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509414.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(656..1414)
                     /locus_tag="IXF88_002732"
     CDS             complement(656..1414)
                     /locus_tag="IXF88_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1509415.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          416 a          277 c          276 g          500 t
ORIGIN      
        1 aaagattgtt cttttacagg gggttcattt tttcagcaag cacatcaaat tgggatggca
       61 gggttcgaac ctacaattgc ttgattcaga gtcaagtgcc ttaccagttt ggctacatcc
      121 catttattca acaaaataat catagcactt tcctaaaaaa acgcaagtta tttttcttga
      181 tcggatagtt gttgctttac tcgtaaaagg cggcgtacta gtttcttttc taatccagca
      241 ggaagggcaa tcagttctgc tactgacaag acggtgtcca ataactgata ttctttgaca
      301 ggttcattca taatatgata gatgtttgct aaacgttcaa tatattcaaa gcgagtatac
      361 tctgttcgag caattagaaa tttcaataaa tcggtggctt tctcgtactc ttgatctgcg
      421 taataaacaa gagattgacg atacaatttg atttccagtt cattgagggt agcaggtctg
      481 cgacgctcgt tccactcatt aatagagact ttaccttgaa catttccctt gcagtcacgg
      541 acaaaaataa tcggaatttc atttggatcc atttttttat gtgtgaattt ttccattatc
      601 tacgctccct tcaaaggttt ggtatcttga gtataaggga tttttgtttg aattactagc
      661 ttttctccaa agttagcatc tgtccaccca ttgcagtgaa aatcgccgaa aaatcgttga
      721 tcggtactgc tcgatttttt acaccatagg gatcgttgac ataaatattt tctttgtcat
      781 atccagtgac tacacaagca tgaacaagag gagtgactct tacttctcca ttatttgtgg
      841 tccaagtcat aaaatcagtt tcatctggga cttcaaaatt gatagtcaca gaagtccaga
      901 caggatttcc tttagatagt gcttttttga tatcatcgag ttgagcatcg gtttttggaa
      961 aagtgtgata atccgtttgg acgatttgct gagcaaggga ggcaactggg ggaacataga
     1021 cgaccatagc ccagtctccg ccactgatat caccaacaaa tccttcatta ggatcgccat
     1081 gagtcccatc tgcattgaaa acagggacgt aatccaattg ctcagctaac tggtttttag
     1141 ttgtttcata accataaaaa gataaaagca tagaaagcga agtgacttca cagccatttt
     1201 ctaaagaagg agaatcaaac tggctctcta atggaacatc tagtaaaact tctgctttct
     1261 cttttgtctt tttcgttttt tgtgttgaac ttttttgtgc agtgttcatt gtatgttctg
     1321 gagaatggat atgtaggtaa taacttgtac cgcttaataa aagagcagct gtgcccccta
     1381 gaaacaatag ttttttgaac gtttttttct tcatttgaaa tctccctttt cagctgttta
     1441 tctttcctat tataaatgta tttttattg
//
LOCUS       DACESX010000243         1462 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.248, whole genome shotgun sequence.
ACCESSION   DACESX010000243 DACESX010000000
VERSION     DACESX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.248"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            851..1063
                     /locus_tag="IXF88_002733"
     CDS             851..1063
                     /locus_tag="IXF88_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1509416.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1053..1448
                     /locus_tag="IXF88_002734"
     CDS             1053..1448
                     /locus_tag="IXF88_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509417.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
BASE COUNT          508 a          215 c          247 g          492 t
ORIGIN      
        1 agttataaga aatgagtact ctagtatttg tgttacccga ataattactt gattatattg
       61 gtttcccata taattgaagg ctgtcctaat ttttacctac tatatgatca taattgatta
      121 actcttatat atttccgaaa attatcttga agatttctat tggtatggag aatatccatt
      181 tcattgccaa taaggatata aattctcaaa ataacaatcg atgtctgtat taaacaagcc
      241 tagaatggta aatctgattc gccaaaacca ttggattgta tcgatttgct ttacgcgaac
      301 gccacctacc cattttataa aaataaaatg ggcaaaagat ctttcttttt gtcatacaaa
      361 aagaagaaaa aaaagagacg attttcttct tccaaaatcg tccctctttt ttctaaaacc
      421 atggatgaac gaacaaaaaa tctctcgtat tttgtttgtt catccactaa aaatatgcga
      481 acagtatggt ttcattcaat cctgttctta caagtcaaaa gcatcgaatc gtccttggac
      541 gattcaacaa aaaaagtagt cgtttttgga tgactactaa gttctaaaca ttcattaccc
      601 aaaatcaatt tggtctaaca tcgtttctcg actggcttga tctaatcggc tcttgacagg
      661 gctttttctt ttctgttgat agcagataag aggaaataag aataagtgtg ttcatgtgtc
      721 ataaaaacag taactaaaac attaaaatca aaatttatat ggaaagcttc cttgacattt
      781 tgtgctcgca ggagtattat aaaagcacga aaggaaagta aactttccat tttttagaaa
      841 ggagcatgtt gtgaaaaatc gtttggaaga attacgaaaa caaagaggaa ttaaacaaga
      901 agatttagct tctgctcttg aagtatctcg tcaaacaata ggttctttag aaaatggacg
      961 ttataatccc tctattatat tggcatttaa aattgcaagg tactttcaaa tgagcattga
     1021 agaaattttt atctatgagg aggaaagtga agatgaaaaa taagggtcag atttggtata
     1081 tcggttatat tgtttcgttg tttttgattg tgacaatttt ggttacagat tttcctaaaa
     1141 tggtagatat tggacttgct attttattca gtgccgtttt tggcgtttct catgttcaaa
     1201 ttttgcatga taaaatgatt aaaaatgatg cagactacaa aatcaatgtt atggacgaac
     1261 ggaatatcat aatcaaagaa aaagcgggaa atataacaaa tatgtttaac acaatactgt
     1321 taggggtggc aacagttata tttatttgtt tagactacat cattccagct attattgtcg
     1381 gaagtatcat tgcaatccag ccgattattt taatcattat cagtagtgtg cttgagaaaa
     1441 aaatgtagaa ataaaggttc at
//
LOCUS       DACESX010000244         1462 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.249, whole genome shotgun sequence.
ACCESSION   DACESX010000244 DACESX010000000
VERSION     DACESX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.249"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            77..487
                     /locus_tag="IXF88_002735"
     CDS             77..487
                     /locus_tag="IXF88_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1509418.1"
                     /translation="MIKVYTSPSCTSCRKAKAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            501..857
                     /locus_tag="IXF88_002736"
     CDS             501..857
                     /locus_tag="IXF88_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1509419.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          540 a          211 c          270 g          441 t
ORIGIN      
        1 tattttatta gctaataatt ggtattttaa aagaaattac tgtagaattg gattataatt
       61 aaaaaggagt gagaaaatga taaaagtata tacttcgcct agctgtactt cctgtagaaa
      121 agcaaaagca tggttgcaag cgaatcaact tgaatttgaa gagaagaata tttttgccga
      181 accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag gagttcaaga
      241 tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta acgaattaac
      301 tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac gacctatttt
      361 gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt catttgtccc
      421 tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata acatcttaaa
      481 ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac tgtgtaacaa
      541 gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt gttgacttag
      601 aagaacgcga tctaagtaca gaagaagcag aagaattatg cagttttgaa gaagtgcaag
      661 cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt gacctttcct
      721 gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc ctcagacgtc
      781 ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg atggaacagc
      841 ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt tcatacagtt
      901 gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac tttatttttc
      961 tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg ggatatagag
     1021 ttaaaaatat attgacatgt gtaaatttac atgatatatt catttctgtt gttttaagta
     1081 gcaaagcaat aaaatagtat tttaaaaaac atataaaata gttgttgaca tgttagaaat
     1141 gacatgataa gataactgag ttgttaaaaa agaaccactt gtgaagcttg attctatcga
     1201 aaaaaacttc aaaaaagttc ttgacaaata acaaatgata agttatgata aatgagttgc
     1261 tgtaagattt cgaaaaacga aatcaaaaaa acttttaaaa aagagttgac aattacttgt
     1321 ctctctgata tgatataaaa gttgctacgg cagccaaaaa cgaatcgggg aacactcctg
     1381 atgaagtaga cctttgaaaa ctgaacaaag taagacaaac caaatgtgta gggcgtcttg
     1441 attcaattca agacaacaaa ca
//
LOCUS       DACESX010000245         1416 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.250, whole genome shotgun sequence.
ACCESSION   DACESX010000245 DACESX010000000
VERSION     DACESX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.250"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            104..322
                     /locus_tag="IXF88_002737"
                     /pseudo
     CDS             104..322
                     /locus_tag="IXF88_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            582..836
                     /locus_tag="IXF88_002738"
     CDS             582..836
                     /locus_tag="IXF88_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1509420.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            837..1154
                     /locus_tag="IXF88_002739"
     CDS             837..1154
                     /locus_tag="IXF88_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1509421.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          221 c          278 g          424 t
ORIGIN      
        1 atgaaattct agatattttt tgctacatag agttactttc aatatttttt aacgtaatta
       61 ggcagaagtc actcatcctc tataggtttg tgatgaaatt gctattgatc catttaagta
      121 agagcaaaaa actggaggtt tatcaggtaa ttggtctaat ggatttaagt atgcggtcac
      181 cgtgtataga gttgcttatg agattcaaga aaataaagtt attccagtat tgctatatgg
      241 aacacataac aatttttatg aattagccga gaacactcgc aactttattc gtgtagatga
      301 atcggcttcg gttgagttat aactttagct atatcaatca accgaccgac atatgttaaa
      361 tctatatata ataaaaatac ctacttaaat ggatgttaac ggaagtgtat ggtgagtagt
      421 tcactcgtat tttgatatcg tttgtatatg ttttcagcgt tctaagtggc ttattttgtc
      481 caataataat atggagtgga atcccttaag aaacaccgtt ggcaattgtc gttataacat
      541 gttataattt atttatcaaa aactaaaaga agtgtgataa catgggaaaa gaactaaagg
      601 ttagaaagat cggcaatagc gtgggagtga tccttccaag ctctcttgga ttaaaaagcg
      661 gagacactat ccaagccaaa caagaaggca acttattcat attagatact actcaaatag
      721 caaaagaaca cgatcgcaaa ttgattgaag aaagtttcca agactttgag aaaggtttaa
      781 cagtttcaga aattgaaatg gtaaaagctt tcggaaaata cggctggagt gaatagatga
      841 aagagtacac gctagtttat tctaaaagtt tccagaccag ccttgaaacg ataatagaag
      901 aatgggaaaa tgagcttgct ctttctccag ccagcataaa aagatttgta ggtgcgatcc
      961 agaaggcttt agaactaacc aaaaattttc caactatgca tgaggaagtt tccgaaatct
     1021 atggaatgga tatcccaaca tatcggatcg tgatcggcaa aagctatgcc ttattttatc
     1081 ggatcgacca tgaaaaaaaa gagatacttg taggacgtat tttccaatct aaacaaatga
     1141 aactaggctt ttaacaataa ttaggcaaaa gtcgctcatc ctctacaggt gggtgattaa
     1201 tgccgctcga tatggttgtt aaccgtcggt tactcgaaga ttgatatata agacttagta
     1261 cctttttatt ttgctccatg ttatggccga aggtctaaag atacaatcat tagtctagag
     1321 aaaaattaag aaccgtagga actacgggga tcgcttggtg aataagagac actgctatta
     1381 gtgaaaaaat aagatatcaa gtacgctcta ttccca
//
LOCUS       DACESX010000246         1404 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.251, whole genome shotgun sequence.
ACCESSION   DACESX010000246 DACESX010000000
VERSION     DACESX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.251"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            153..767
                     /locus_tag="IXF88_002740"
     CDS             153..767
                     /locus_tag="IXF88_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1509422.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(791..1141)
                     /locus_tag="IXF88_002741"
     CDS             complement(791..1141)
                     /locus_tag="IXF88_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1509423.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1135..1398)
                     /locus_tag="IXF88_002742"
     CDS             complement(1135..1398)
                     /locus_tag="IXF88_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ1509424.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          445 a          263 c          230 g          466 t
ORIGIN      
        1 aaagaaaagt gacacatact atttcctcca tcattagttt ttgtctatat aaggtatacc
       61 ctaactagac acgaccgcga cattgaaaaa tctggttttc tcgtgtatga ttagggtgtt
      121 ttttaaatag ttgcagaaag taggtaaaaa ttttgagagt tgcatacata cgtgtctcat
      181 ccatcgatca aaatgaacaa cggcagattg aagaaatgaa aaaattcggt gcagagagaa
      241 ttttcattga gaaacaatct ggcgcaacga ttactcatcg acctgttttt caagaagcac
      301 tggatttcgt ccgagatcaa gatattttta ttgtggaagc tattgatcga cttggaagaa
      361 attacgacga gatcatcgct tcagtcaatt acttgaagaa gaaaaatgtg cagctgatca
      421 tcacaagtct accgatcatg gccgaagcaa ttggcaatcc tctcctggat aaattcatta
      481 aagatttgat cattcaaatt ttggccatga tcgctgaaca agaacgtacc gaatctaaac
      541 gacgtcaggc acaaggcatt aagattgcaa aagcgaacgg tgtttataaa ggacgtccta
      601 agctatacag tgccaatgca aaagatcccc aacgtcgtct tgtttataaa aacatcgttg
      661 aggatctaaa aaaaggtgtt gcgatcgcta agattgcaaa agactacaat gttacgagac
      721 aaaccgtata tagaataaaa aaagacagca tggtcaatca cgaatagttg atcatgctgc
      781 tgtttctatt ttatttgctt actttttgaa aaactgttct aactttcatc aataactcct
      841 caactaaact atcatgcgct ttttccaaaa aaatacactc tctggcttca taatcaattg
      901 tcgttagttg atctaataat acttttcctg ttgtcttcat ttcatattct gagtttatat
      961 ttacatatag cggatagttt ctagttgtac tagaaattgg agcaactgta acaacgttac
     1021 tataatccgc aataatatca tgacttaaaa caatataagg tcttttacct gtctgttcgt
     1081 gtccagatcg aggtgctgta ttcaataata gaatatcgcc ttgtctaggt tttctcacca
     1141 tttttcatta cctactgctt cacctaaatt ttgaatcgtc acatgatttc tttcatcagt
     1201 gtaattttca aataactgtt caattgttaa ttcattttct atattagccg cttcgataat
     1261 tagccgcttc tgaccgttta cttcttcttg tattatcttc actttgtctt ctgtatgaat
     1321 tccaagatat tccataatat actttggcaa tctaaggcca ttactatttc cccatttttt
     1381 tataggtaac atttccatag taga
//
LOCUS       DACESX010000247         1393 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.252, whole genome shotgun sequence.
ACCESSION   DACESX010000247 DACESX010000000
VERSION     DACESX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.252"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            28..885
                     /locus_tag="IXF88_002743"
     CDS             28..885
                     /locus_tag="IXF88_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1509425.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            886..>1393
                     /locus_tag="IXF88_002744"
     CDS             886..>1393
                     /locus_tag="IXF88_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509426.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTED"
BASE COUNT          531 a          201 c          296 g          365 t
ORIGIN      
        1 tattgctaaa agaaaagagg taggaacgtg gtagcgaaga aatatgtaat cggtaatttt
       61 aaaggtggcg taggtaaaag tacgtgcgcg caaatgttag ggtttgaatc agcagttaat
      121 gaaggaaaaa gaaccttgat aattgacttg gatatgcaag gaaatacttc tgatgtgatg
      181 aacttaacgc atatgaactt cactaacgaa gaaggaggcg gagaaggtga accgattgtt
      241 tacgaaaata ctgtgactga cgtgttgata aacaatcttc cacctaaaga ggctgtctat
      301 aaagtaatca ataacctcta tatacttcca gcagacatgt cctttgaaat gtatgatgat
      361 tggatcaaag accaatataa tgattcatta tcccaattca aatatatgaa agaaaagcta
      421 gaaccattat ttgatgagtt tgacgtcatc tatttagatg tcccaccttc tatttctgta
      481 tatagtaaat ccgcgatgta cattgcggat tgggcaatcg tcgtcttaca gacccaagta
      541 aaatcgatga gaaatgcgat gcaatatctt gagtatatgg acttttttgt gaaagaattt
      601 gatacaaatt tatatgttgc tggtgttatt cctttcatgt tggaaagcgg tgacgcagta
      661 gatcaggaaa tgtataaaca agcccaagag atttatgggg aacatttgat taaaaatgtt
      721 gtactcaaaa atgcgcgctt aaaacgatac gacggatcag gtattacgat ggagaagaca
      781 aagaaaggtc tcttaaaaca atgggatcgc aaaagccacg aattatttat cagtattttg
      841 aaagaattgg aagaacacga gaattggtat gtggaggtgg agtaattggc tgacatcaaa
      901 ggacttgata tttttgggaa caacaaagga ctcgccaaaa taaaaaaaga agcagaagca
      961 aagaaaaagc agcaagcaat tacaccatct gaaaataagc aattagaagt gcaccaagtt
     1021 caagaaataa aaacttcctc caatgttgaa agacagagta gaggaagaac aaaaaaagtt
     1081 ggcgcaccaa tcagaaagtt tgataaggtt tattccacca agcatccttt aaaactgtcg
     1141 actttgttaa atgcgacttc cagagtttta gtagaaaagt atgagactaa tatgacaaga
     1201 gatgaattgc ttagaaaagc tttagatgag tatattaaac agaatcttac gcaagaagat
     1261 aaacaagatt tgtttaatga cgtggtgaaa gaactagact tgttcagaga aaaatatcca
     1321 attgttccag aattagatga tcgtggagaa ataataagaa ctaccagtca gataaaacaa
     1381 gataccgaag act
//
LOCUS       DACESX010000248         1364 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.253, whole genome shotgun sequence.
ACCESSION   DACESX010000248 DACESX010000000
VERSION     DACESX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.253"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IXF88_002745"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IXF88_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1509427.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DACESX010000249         1355 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.254, whole genome shotgun sequence.
ACCESSION   DACESX010000249 DACESX010000000
VERSION     DACESX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.254"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(7..1194)
                     /locus_tag="IXF88_002746"
     CDS             complement(7..1194)
                     /locus_tag="IXF88_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1509428.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          396 a          285 c          224 g          450 t
ORIGIN      
        1 atggtgctaa atgttattcg aattaaattc taaatatttt cgtggagaag ccagccactc
       61 agtgtctttt tccatcaaaa gtgttccaat tatccgagtg atggaattga tatttggaaa
      121 aatccgaatc actcgttctc tccggcgaat ctcttcgttc aaccgttcca acacattggt
      181 tgtacgaatc cttcggcgga tattttcagg aaaagacaaa atttgaatag catcttcaaa
      241 gccgttttct aaaatgtcgc aagcagcact taattttgaa tcacaaccat atttttccaa
      301 gaagtgctct tttctttcac gtgtcaattc aagttcagag gctttaaaga tactcttcaa
      361 ctcgtctttt acatcagaag aaactttctt tggcagctta tcaaaaatat ttctcaaaaa
      421 gtgggcttgg catctttgcc agctcgcacc taaaaagttc ttgcgaacag ccttaactaa
      481 ccctttgtgt gcatcagaaa tgaccagttt tacaccgaac aaacctcgtt gaacgaacga
      541 ttgaaaaacg gtatcccaat tatcttccga ttcgccgtct tgaacatcaa aaccaagaat
      601 cgtacgtcgt ccatcagaat caatgccgat accaatatat acaccttttg agacaatacg
      661 atgattttcc cttactttca tatacagggc atcacacatg aaaaatggat attccgagcc
      721 ttctaggctc cggtttctcc actcaaaaac aaatggatcc aactgtttca tcagatttga
      781 aacgaatgat ttagaaacag tcgcaccatc cgttagcagt tcaactgttt tagttacttt
      841 gcgagtggag acaccagaaa tgaccatttc aatcatggca gcgatcaaag ctttttcgct
      901 tcgctgatat ctttcaaaga tagtaggact aaacttaccg tctctggttc gtggaacatg
      961 aagttctaac gtaccaattc gagttgtaaa cgaacgatcg tagtaaccat ttctttgtga
     1021 ttgccgttct tctgaacgtt cataggcatt tgcctgaata tattgatttc gttgttcttc
     1081 catcagctga ttaaacatga tagtcataag tgtttttgcg gtatcattcg caccgctttc
     1141 gtttataatt gcttgaattt cttcgctttc aaaagtaaaa tgtacttgag tcattgatat
     1201 cagtcctatt cgagtatgtt ttctgcaaaa aacattgtac tcctaactga gatcaatggc
     1261 ttttttgatg cttcactttt acacaattat atggacgtaa tcaaaagaaa tgctttttta
     1321 cttattttca tagatttgtt gttctccttt tttaa
//
LOCUS       DACESX010000250         1335 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.255, whole genome shotgun sequence.
ACCESSION   DACESX010000250 DACESX010000000
VERSION     DACESX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.255"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            5..739
                     /locus_tag="IXF88_002747"
     CDS             5..739
                     /locus_tag="IXF88_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1509429.1"
                     /translation="MSLAAALLFAAGCSANNTTSSSSSTESSKSTSTAESTTASSVDL
                     NALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAKDGYYNGLTF
                     HRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARSQDPNSNGSQ
                     FFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDVVDAIANI
                     ETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(854..1294)
                     /gene="fabZ"
                     /locus_tag="IXF88_002748"
     CDS             complement(854..1294)
                     /gene="fabZ"
                     /locus_tag="IXF88_002748"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1509430.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          436 a          266 c          252 g          381 t
ORIGIN      
        1 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
       61 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
      121 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
      181 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
      241 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
      301 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
      361 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
      421 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
      481 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
      541 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
      601 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
      661 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
      721 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
      781 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
      841 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
      901 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
      961 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     1021 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     1081 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     1141 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     1201 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     1261 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     1321 gccattttca ttact
//
LOCUS       DACESX010000251         1313 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.256, whole genome shotgun sequence.
ACCESSION   DACESX010000251 DACESX010000000
VERSION     DACESX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.256"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(604..1200)
                     /locus_tag="IXF88_002749"
     CDS             complement(604..1200)
                     /locus_tag="IXF88_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HAQ1509431.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT          409 a          264 c          179 g          461 t
ORIGIN      
        1 tatggatcca ataaagtaga tatggaatat ttattttctc ttgtctaatt tataagaact
       61 aaatgatttc atcattgttt tgacactttt agttattcga caacaatcat tttgtgataa
      121 tacataatta accttatatg aataccaaaa aaataattta tctttctcta ttcaacttat
      181 catttacatc tgtaaatttt ttgttgacaa tttaaatcta caagagtaga cttttctcat
      241 actattaaag aagagataga tttataggaa taggaggcac ctcattaata tatgatttgg
      301 ggttatatga agagttgatt aagaaaccaa ataaaattga agggcaagca gccaatatca
      361 aaagaataat aaatgcgaac aaaaaccagt taagcactct gcctaactgg ttatgactaa
      421 attttcagtt gtctctctac cagcactaaa tgtcgaagag ccaaaaatct gagccagaat
      481 gaggttcgtt ctgacccaga tttgattttg cacaactcat cagtccttat taacaaagag
      541 ctcatattcc tcatgttgag aatacgaaga ccatgtacca gcttgttggt ttagtatata
      601 aaattactca ttattttcgt catattccat ctcatcttca gttacccaca tatgattctt
      661 aacttcttct tgaccatcgg ttggagtgta attaacaacg taaacagttc ctgtaataga
      721 tttatctact tttgcttcag ctcccatcat cccatccatg tgatcggcat tcaagataac
      781 agtatcgccc tcatttgcta cagtctcagt atcttttaac tcttcttgta ctacccatcg
      841 atgattgtta accattggtc ccccagtttt tggctcatag cttacttcat acattgtagt
      901 atcaaaggcc cctacaactt gagcatttgc acctttcatc ccttccatat gatcgcctag
      961 cagtataaca ttacttccca ccgggaattt agggttcgca gcatctttca ttgaagaagg
     1021 aaccatacct tcgtgattca ttccctccat actattcatt tcactagacg ctgtagaagt
     1081 cgattcctcg tttgtgctat tagaattatt cgattcactt tgattgctac acgctcctaa
     1141 aaatacaata attccaagac ttgcaaatac taattttgtc atctttttca ctgtcttcat
     1201 cctcctttaa ttaatcacat ttaggtcatc tcataaattt gtaatccaaa aacttctttt
     1261 ttaattgtag ctattgataa gtccttctta gaaaagatta ctacagaatt tga
//
LOCUS       DACESX010000252         1289 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.257, whole genome shotgun sequence.
ACCESSION   DACESX010000252 DACESX010000000
VERSION     DACESX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.257"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(15..1154)
                     /locus_tag="IXF88_002750"
     CDS             complement(15..1154)
                     /locus_tag="IXF88_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1509432.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          336 a          281 c          239 g          433 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttatt
//
LOCUS       DACESX010000253         1280 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.258, whole genome shotgun sequence.
ACCESSION   DACESX010000253 DACESX010000000
VERSION     DACESX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.258"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            103..716
                     /locus_tag="IXF88_002751"
                     /pseudo
     CDS             103..716
                     /locus_tag="IXF88_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          455 a          224 c          202 g          399 t
ORIGIN      
        1 ttatttcgaa attttctgat attatattcg caacattagt gaaaattctt agataaattc
       61 attctttcaa tctccgaatt ttagatatat gtgaaatatc tgttttttga atgtttgcat
      121 aagttaaaag atgagcgtgc tttagtattc tttagaatag cttcttatac aggggaacga
      181 aaaggagaaa tactcggact gacatgtaga gacatagatt tcgataatca aactatctgc
      241 ttcaacaaaa cgttagtaga actatccgag ggctctctat gtattaatcc tacaaaaaca
      301 gacaaatcaa agggagtaat ttcaattgat aatggaacct tacgtattct aaaaaatgga
      361 aacgttctgt aacataaaag aacctttcta gagggattcg gatagataat atagatgata
      421 tacctgtaat cagcactatc aaagggaata aaaaaaggga cttcctttac aaaggctacc
      481 ctaaccatgt catggataaa gtagcacgga agttcccaga tatgaagata atcaaagtcc
      541 atggcttcag aaagactcat gctacccttc tctttgaagc aggtgctcaa gtaaaagatg
      601 tacaagatag gctaggacat tcttcttcta aaacaatcat ggacatttat attaaagtat
      661 caaaaaatcg gcaaaatgaa acagtcgaat tatttgattc ttacatgaac ggatgataag
      721 cgaggtcaaa aacggagtca gaggatatta tggggatacc tttttacctc ttaatccttt
      781 aatatcaatc ctttaagctt cgacttttgc aatcttccct tgttcgatat agacccaaat
      841 cgatctatca ataattatca aactgagtca tatagagaca aaaatttcaa aaacgaccta
      901 ttaattggtc atcgaatatc atttaaaatc aatacgacgg agtcaaacac ggagtcaaaa
      961 caagcccgct gagagtaaaa aatatacagt cccgtaggta aagtgccaca ggcctgtata
     1021 ttttttattt gagattacct gaaatgattc catttacttt ttatctcatt ttttcctact
     1081 agttagtatc aaatactctc ctattttctc aataaactat tttgggttac atggtatctg
     1141 aaacagctaa aaccttgtca ttacagcaaa aaatcacttt atctctgttt catttcagat
     1201 acatttcact taagcggatt ccaaaaaatc aaaagaaaaa ggaaccccta gttagcctat
     1261 gctttttatt ggtttctctc
//
LOCUS       DACESX010000254         1258 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.259, whole genome shotgun sequence.
ACCESSION   DACESX010000254 DACESX010000000
VERSION     DACESX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.259"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(1..1095)
                     /locus_tag="IXF88_002752"
     CDS             complement(1..1095)
                     /locus_tag="IXF88_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1509433.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          363 a          267 c          194 g          434 t
ORIGIN      
        1 ctatttttta ttttcaatta atttaaagct tgaccatggt ttcaaaaagt caaggaggaa
       61 taattcgtca tcggagattc ttcctacaac attgacacgt ccgtcgtttt tcatatcctt
      121 caaggcgatt tgtgtttctc ctttgtattg accgtaccct tcattgtcaa tcagtacatc
      181 cccatgatgc atatctcgcg tattatgagg cgggaaatct ttatctttgt agtatacacg
      241 agtcatcgtt gaacgaagga tatattctga gcgatcccct cgataactat gaaggttatc
      301 aaacaagcag attcgttcgt tttctgtaat accttcttcc gtatctactt tcaatgttgg
      361 ataatccgcg ttaaatgctt ctgccatttc tttcaattct tcttcacttg cgtacgcatt
      421 tccaatagaa atatcatcaa tcaatcctgt aaggattaaa tgcttcactt gtgtcgcaat
      481 ttcaagatct cggtggtctt ccagcgtgca caagccatct tgcgtaggcc aagggccaaa
      541 ctcagcagat tgcgaattga cgaatgccat cgtattcaaa ttatattttc tgaatttttc
      601 tgaacaaaaa acaaagtgtt cgtatcctaa accagagtat cgatgaggat aaaaattatg
      661 agaacctaat aaattatccg tgtttggcga ataactcatg atgttgtcaa cgtaattcgt
      721 gcccgaactc atattgatct cgattttgat accaaaagga ttgcgcgtca tttttgcttc
      781 ttctgctcct gtgaatccga tatctaagcg gaccccgtag gcacccatct tatggaaaaa
      841 agataagtca tcgtaagaaa tctccaactg ttcaaataac gctggattga tatcaaccat
      901 tacttccatg cctaggctat tcgcgtaatc aactacttct ttaaattctg aaagaacttt
      961 ttctttatca tcattgatct gcagcaaact ggtaaaaacc cgtttatacc catacttatg
     1021 agctaaatcc aagtacgctt tgtccttttc aaatgttgac cgttctggat aaatcgagat
     1081 tcctaatttt cccatattac catttcctcc tgcatctttt cgtttttgta tccacttaca
     1141 ttatagcaaa cacaaagtaa atggtctaca ccttttcttt ttcttttaaa ataaatgata
     1201 aattccaata atttaagtag gtattttccc tctattttgt tatcattaaa gataacaa
//
LOCUS       DACESX010000255         1242 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.260, whole genome shotgun sequence.
ACCESSION   DACESX010000255 DACESX010000000
VERSION     DACESX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.260"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            79..552
                     /locus_tag="IXF88_002753"
     CDS             79..552
                     /locus_tag="IXF88_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1509434.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            561..788
                     /locus_tag="IXF88_002754"
     CDS             561..788
                     /locus_tag="IXF88_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1509435.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          392 a          198 c          207 g          445 t
ORIGIN      
        1 gtttttcgat aaaaaatagt tgttttacaa taattattct gctatatttt agctatcaaa
       61 gataaaagag ggataaaaat gaaatttaaa acattgctat taaaatttgt cgttttaggt
      121 ataagtgccg tatttatttt gttcggcgta cttttttata ctcagatcgc tacaagcgaa
      181 aaaggattca attttgattg gcagaccaca ttatttgttt ctgtcattta ctttgccctt
      241 ctcctaggga tcgttattgc ttatttctta ttgaaactat tgaaaaaaat cgaagataac
      301 caagcttttt ccactcaaag cttaaacata ctaaaaaaaa tcgaacgaac cattttgttt
      361 attggtttcg ccttttttgg cttacttcct aaatttatga aagtagcgga tttagacgat
      421 gctccaggtt tgatgttggt aggaatcgct gtcgtattca ttccttttgc agtatacaca
      481 ttaacaagcg tgctggaaaa actgcttgaa catgcgattt tacttaagac agatcatgat
      541 ttgacagtgt gaggtgcgac atggcaatta tcgttaattt agatgtagta ttagctcaaa
      601 aaaaaatgag tgtgactgaa ttgagtgaga aagtgggcat tacgatggca aacatctcta
      661 ttctaaaaaa tggaaaagca aaagccatcc gtttttccac tctcgaaaaa atctgccaag
      721 ttttggagtg tcaaccaggt gatttgttga cctataaaaa tgactcgaat actataccag
      781 aagaataaaa taaagtagaa caaacagtaa ataaaaaata atctgaatga tagatgtgtg
      841 agacttttag aagttattta cgtctattat tcagattttt tatttcgatt gaataccgtt
      901 ttatctataa ataagatatg tactgactca tacaaagggg gccttttctt ctccatgtac
      961 tatcactttt cctgatacaa ctggtttcct gttacgttgt agttggatct gcttttagta
     1021 taatacttta ttcattccac cagactgata gcagtttgag cagtctaaat aatcatgtag
     1081 tattacaggc cattctgttt cataccttga agtgcttaaa acttcagcta atggcccatt
     1141 gtgttaactc ttactccatt cttttttagt aagctcccat atatactgta aagccttcta
     1201 tatattatgt catgttgctt tttaacattt actatgtagt tt
//
LOCUS       DACESX010000256         1240 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.261, whole genome shotgun sequence.
ACCESSION   DACESX010000256 DACESX010000000
VERSION     DACESX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.261"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            21..893
                     /locus_tag="IXF88_002755"
     CDS             21..893
                     /locus_tag="IXF88_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1509436.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          374 a          228 c          300 g          338 t
ORIGIN      
        1 aaataaaggg agaaatgtct atgctatacg gtgcaattga agcaggcggc acaaaatttg
       61 tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt ccgacaacga
      121 cacctgagga aacaatgcct ttagtcattg acttcttcaa acaataccaa gcagatttag
      181 caggaattgg gattggctca tttgggccta tcgatatcca tcgcgattcc gccacttatg
      241 ggtacattac ctcgaccccg aaattggcat ggcaaaattt tgatttcatc ggtaccatga
      301 aaaaagaatt tccgatcccg atttcttgga caacagatgt caatgcggct gcctatggtg
      361 agtatgtctt cggcagtggc aaaggattga gcagtgtcgt ttactatacg atcggtacgg
      421 gtgttggcgg cggtgcctta caagaagggc gctttatcga agggtttagt catccagaaa
      481 tggggcatat gctcgtcgtc cctcatccaa aagatgactt tgctggcagc tgtccatttc
      541 atggcaactg tttagaaggg atggccgctg gacccgccat cgaaaaccgc ttagggaaaa
      601 aaggacaaga ggttgcagaa gatgatcctt attgggaaat cgaagcctcc tatattgctc
      661 aatgtgccta caatacaacc ttgatgtttt ctccagatgt gatcattttt ggcggtggtg
      721 tgatgaaaca acgccatatg ctgcaaaacg tccacgacgc atttgcaaaa ttggtcaatg
      781 ggtatgtcga aacacctgag ttaagcaaat atatcgtgac cccagcatta gaagacaacg
      841 ctggaacgct cggttgtttg gcattggctc gagaagcggt cttacattca taaacgaaaa
      901 aaggctcttc gacatttagt gttggtagag aaagatacaa ctgaaaattt agtcataacc
      961 agttaggcag agtgcttaac tggtttttgt tttacttgga agatcaagcc ttgaatgaga
     1021 taaattcttg ctcgaaagtt atggaaattt cgatagccgt aggccacttg tattgtgcaa
     1081 tagtttttta caaaaaaaag ggggtgcgga tatttttttg gaccttttta acaaataggt
     1141 tctgttgcaa agttttaaat ctactatcag ataaggtaga ataatagaaa aagatagcag
     1201 gaggaatgac gatgagtcat tttaaaggaa agcaatttca
//
LOCUS       DACESX010000257         1225 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.262, whole genome shotgun sequence.
ACCESSION   DACESX010000257 DACESX010000000
VERSION     DACESX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.262"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            226..1185
                     /locus_tag="IXF88_002756"
                     /pseudo
     CDS             226..1185
                     /locus_tag="IXF88_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          437 a          240 c          220 g          328 t
ORIGIN      
        1 ccttctttca gcattattca aatcaaatgg caacataata gagaaattta tagtatgtat
       61 aatcatctga attcaatcaa atcaaccgga gtcatatacg gagtcaaact atcccactga
      121 gagtttatta aacgcagatt gtaaaattaa gctagacaac taaaaaagtc atttgtgcta
      181 cactcaaaat agttccgacc aaagaattat aaggagtgaa gacaaatgac ctatacccat
      241 cttacaacaa atgaactcgt gatgatagaa gcctatttca atatgcatca atctgttacc
      301 gtagtcgcta agacctcaaa tcgttccaga cagaccatct acaatgtgta caacttcctg
      361 aaacaaggcc aatctatcca tgactattac caacaatatc aaaaaaataa aacacggtgc
      421 ggccgacgac ccatttgttt gccagcagaa caagatgcgt atatccgaaa caaggtggcg
      481 caaggatgga cacccgatgt gattatcggt cgtgctgagt tttcaatttc atgttctgtt
      541 cgaacccttt atcgcaagtt taaaacaaac taatttgatt gctcgaaact acccatgaaa
      601 ggaaaacgga aacccaacgg gcataaagaa cgtcgaggga aacaagcctt caaacggaat
      661 atttcagaac gtctcacgga ctatcctgaa tttcaacagg aatttggaca tttggaaggc
      721 gacactattg tgggtgtcca tcataaaagt gctgtcatca cgctggttga gcgcttgtca
      781 aaagtgatta ttactttaaa acccgaagga cggaaagcac tagatattga aaaagtgaca
      841 actgactggc tacaaaagct tccccgaaac ttgttcaaat ccattacctt tgattgcgga
      901 aaagaatttt cgaattggaa aaacatgagt aatgctaacg atatttctat ttattttgca
      961 gatcctggaa cgctgtcaca acggggatta aatgaacatt ctaatggatt gttacgtaaa
     1021 gacggacttc ctaatacaat ggatttcaat caggttgacc aaaaatttgt ggcggcggtt
     1081 gcctccaaaa gaaatcatat accaaggaaa tctttaaatt atcgaacatc gttagaagta
     1141 tttttgagtt acataagtga ggagcaattg tctaacttaa tttgacattt agaaaataat
     1201 aaaccatatc ccatctattc aaata
//
LOCUS       DACESX010000258         1206 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.263, whole genome shotgun sequence.
ACCESSION   DACESX010000258 DACESX010000000
VERSION     DACESX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.263"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..>1206)
                     /locus_tag="IXF88_002757"
     CDS             complement(<1..>1206)
                     /locus_tag="IXF88_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1509437.1"
                     /translation="VGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKI
                     FEYAIHEQIIEEDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTEGLLATAN
                     NGYLFLDEVHRLSKENQEKLFVFIDTGNYRPVGENVNWHSAKVRFIFATTEKSEDYLL
                     DTFDRRIQISVMLPTFEDRPIRERLELIQLFFQNEADILQKDIIVSKEALSLMLSHPF
                     SGNIGKLKNIIKISCADVYSRTREEPLTIGKNEILIQLNMLESEVESLPIASLLIKKN
                     QKIKTDRIKPFDYDSILEKITQLINEGNFNEKFSSIKNEVQHLVHHIDKDSFLSMSRK
                     LMIQMFRKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRT
                     TYLCNQFLEH"
BASE COUNT          364 a          261 c          189 g          392 t
ORIGIN      
        1 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
       61 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
      121 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
      181 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
      241 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aacttttcat tgaaattacc
      301 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
      361 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
      421 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggctc
      481 ttcccgtgtt cgactgtaca catccgcaca actgatttta atgatattct tcagcttacc
      541 aatattccca ctaaagggat gagataacat tagagacaaa gcttcttttg aaacaataat
      601 atctttttgt aaaatatccg cttcattttg aaaaaataat tgaatcagct ctaaccgttc
      661 tcgaatggga cgatcttcaa aagttggcaa cataacactg atttgaatac gtcgatcaaa
      721 ggtgtctaat aaataatctt cacttttttc cgtcgtggcg aagataaaac gaaccttagc
      781 agaatgccag tttacatttt caccgactgg acgataattt ccagtatcaa taaaaacaaa
      841 gagcttttct tgattttctt tcgacaaacg atggacctca tcaagaaaaa gatagccatt
      901 gttggctgtt gccaataacc cctctgtatc cttctcagcc cccgtaaagg cccctttctt
      961 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacaatttaa
     1021 aatagcaaat ggtgcatctt cttcaataat ttgctcgtgg atagcgtatt caaaaatttt
     1081 tgtggctaaa aaactttttc caacccccgt ggctccattt attaatacac tgagtccgtt
     1141 aggcggatat ttgacagctg caatacattt ttgaatgacc tctctcagac ttccgtcatg
     1201 acccac
//
LOCUS       DACESX010000259         1206 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.264, whole genome shotgun sequence.
ACCESSION   DACESX010000259 DACESX010000000
VERSION     DACESX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.264"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..343
                     /locus_tag="IXF88_002758"
     CDS             <1..343
                     /locus_tag="IXF88_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1509438.1"
                     /translation="FETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNGMLVAQ
                     RLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITELKHAK
                     ELREEWFTNDD"
     gene            333..599
                     /locus_tag="IXF88_002759"
     CDS             333..599
                     /locus_tag="IXF88_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1509439.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            758..1036
                     /locus_tag="IXF88_002760"
     CDS             758..1036
                     /locus_tag="IXF88_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509440.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(1033..>1206)
                     /locus_tag="IXF88_002761"
     CDS             complement(1033..>1206)
                     /locus_tag="IXF88_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1509441.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT          461 a          167 c          241 g          337 t
ORIGIN      
        1 ttttgaaaca tttaaaagac ccaatggtca tgatgaattt ttagaatggt tggaaacatt
       61 accaaaaaaa gatagtgcga agcttttacg tacaatagaa gaaacagaaa agaatgggat
      121 gctagtagct caacggttga aatgggtgaa aaaattagac actaatttgt acgaattaag
      181 aagtaaggta ggatcaaaca tccaaagagc catctatttc catgtggaca atggaaggta
      241 tgtcattact cacggattta ctaaaaaaac ggagaaaaca ccaataactg aactcaagca
      301 tgcaaaagaa cttagagagg agtggttcac aaatgatgat taaaaaattg attgaagaaa
      361 agaaaaaaga taaagatttt gcagaagcat atgaccgtga aggaagaaaa ctcgaaactg
      421 cggttgctct ttatcatgct cgggaggaag cagggctaac tcaagcggag cttgcacaaa
      481 aagcacatac aactcaatct actattgctc gaattgagag aggggataac gtatctttcg
      541 ataaacttga tgatatcgcc agggcattag gtaaaaagct ggtagtagaa tttgcttaaa
      601 agcgagttga aaatatatct ataataggct cctaactaat caattagttt agtgggcctt
      661 tttttactag atagataaag gttgtactat agtgtaaaag acaataaatt ttatactaaa
      721 attactttaa agagaactag agagaaaggt acaataaatg aaaaccataa atcttataaa
      781 agtatttata gcatcaccag gtgatatagc agcaaaaagg aatgaagtag aagaaaccat
      841 atatagatgg aatatagaaa atacagataa tacaaaagtg attttgatgc ctatacgatg
      901 ggaaactaat gcgattccta cttatgtaaa taatagctct gggcaaagtg ttattaataa
      961 acaaattgta gaaaataggg ttctgctgca aagttttaaa aataaaatca gtttttgtat
     1021 tatttcttca ttttaagctg gaattcccaa taatatcctg atttcagtgc agactgaaaa
     1081 gccgaagaga gtgccttctt ttcggctttt cttgtagatt cctcgaatgg tttccatccc
     1141 tttaattgtg gtggaagctg tacgtagact ttgatacaat ttatgtcgac gttttatcga
     1201 tcgatg
//
LOCUS       DACESX010000260         1201 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.265, whole genome shotgun sequence.
ACCESSION   DACESX010000260 DACESX010000000
VERSION     DACESX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.265"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..>1201
                     /locus_tag="IXF88_002762"
     CDS             <1..>1201
                     /locus_tag="IXF88_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ1509442.1"
                     /translation="KKTLVVDAVLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETIVR
                     FEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKVSL
                     SFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIEDKMSFICAD
                     GETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVVID
                     RFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRANINHYEYKTWK
                     SFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFSLLAELPET
                     LDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMRIR
                     IFLINQLI"
BASE COUNT          398 a          238 c          229 g          336 t
ORIGIN      
        1 aaaaagacgt tggtcgtcga tgctgttctc tcgcctactc ctcgtgcttg tagaagttgt
       61 ggttctactg tggtagatgg aaacgggaaa gcaattatag tgaaaaatgg aaaaaaggaa
      121 acgattgtcc gttttgaaca atacaatcat atgcctttgg ttatgcgcct aaaaaagcaa
      181 cgctatacct gtaaaaactg tcgcacccat tggacggctc aaagttattt tgtccaaccc
      241 agacattcaa tcgcaaatca tgttagatat aaaattgctt ctttactgac tgaaaaagta
      301 tctttatctt ttattgcgaa aagctgtcag gtatctttga ccaccgttat tcgtacattg
      361 aaagagttta aaagctattt accaaagcaa tctaagaaga tcctccccag agtattgatg
      421 gttgacgaat ttcgttcgca tgcttccata gaagataaga tgagctttat ttgcgcggat
      481 ggcgaaacag gaaaattaat agatgttttg cctacgcgta aattacctcg attaacaagc
      541 tatttcttag gttgtaccaa tccagaagaa gtagaattct tggtgacaga catgaacgcc
      601 gcctacttcc agctcaccaa acgtgttctg ccaaatgcga aagtcgtgat tgatcggttt
      661 catattgtca aacacatgaa tcaagcgttc aatgagttgc gtatccgtga aatgaatgaa
      721 cttcgtaaag ccggacagaa aagccaggca gaaaaactga aaaagaactg gcgctttttg
      781 ctaaaaaatc gtgcaaacat caaccattat gaatacaaaa catggaaaag tttccgagca
      841 ccaaaatacc catttcttac tgaagcaatg atgattgatc gattgcttga gttttctccg
      901 cccctaaagg aggcgtatcc cttttttcat gaattagttg aagcctttcg agacaaagac
      961 cctgacttat ttttctcctt attggcagaa cttcccgaaa cgttagatga cagctttcgg
     1021 gaaaagcttc aaaaccttct gacctatgaa gaaggcatca ccaacgcaat gatctatcct
     1081 tattccaatg gaaaaataga agcgaagaat acccacataa agacaatgaa acgagtatcc
     1141 tacggattta aatcatttga gaacatgaga attagaatct ttttgatcaa tcaattaatc
     1201 a
//
LOCUS       DACESX010000261         1180 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.266, whole genome shotgun sequence.
ACCESSION   DACESX010000261 DACESX010000000
VERSION     DACESX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.266"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..701
                     /locus_tag="IXF88_002763"
     CDS             <1..701
                     /locus_tag="IXF88_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1509443.1"
                     /translation="KELQREIMPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITK
                     NFPSNIPNNYLEYYLYPDMVVEHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVL
                     NFNFGEHGQYIVDIAISLLNDDRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEP
                     ITLRKPISDFHKGLMEAQVAVEKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILD
                     EMIKVNKKYWPALK"
     gene            714..1172
                     /locus_tag="IXF88_002764"
     CDS             714..1172
                     /locus_tag="IXF88_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1509444.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT          440 a          174 c          244 g          322 t
ORIGIN      
        1 ataaagaatt gcaacgagaa attatgccag agattattga aaaactaata acaaaagaaa
       61 tgcaagtcgc cgattttaat ataggagata aaacttggca aaaaaccttt caaatgatgt
      121 cagtcatcac gaaaaatttt ccaagtaata tcccgaataa ttacttagag tattacttat
      181 atcctgatat ggtcgttgag catactgaca aagagtatac acgtgctaat atggtcatgg
      241 atggacgaga gaaaaatacc aaagaaatgg ctgataaaat acgccgcggt attgtggaag
      301 aagtattgaa ctttaatttt ggggagcatg gacaatatat cgtcgatata gctatttcct
      361 tgttgaatga tgatcgtcgt cgctttatgc taatcgttcc taatcaaggg gcgattccga
      421 atctacggtc agatgcagtt gttgaaatac cggcatatgt tggtgcaact ggtgtggagc
      481 caattacact aagaaaacca atcagtgatt ttcataaagg attgatggag gcacaagttg
      541 cagtagagaa gttactagta gatgcttact ttgaaggttc ttatcaaaaa gctttacaag
      601 catttacatt gaatcaaacc gtaccaaatg ccagagtcgc aaaaaaaata ctagatgaaa
      661 tgatcaaagt aaacaaaaaa tattggccag cattaaagta gaggtgataa aaagtgcatt
      721 tctttaaaac aagaaacatt ctaaaagctg tagctagtgg tcaaattatt ccattaacag
      781 cagtaaaaga cgaggtcttt tcttcaaaaa tgataggagt tggatacgct ataacgaatc
      841 atgatggata tatccatgcg ccagttaaag gaataatcga taatatattt cctacccgac
      901 atgccataac gatcgaaagt gaatctggca gaaaaatact gatccatatg ggtttagata
      961 cagttgaact cgaaggaagt ccgtttcaga tacgggttga aagtaaccag gaggtaaaag
     1021 aagggacact gttagctatt atagatttga agaagttgga gcaagcacaa aaagatacgg
     1081 tgatcattgt agtttctcca ggcatttttg aaggaaattt attaaaacaa aaccaagaag
     1141 taaagattaa agatagttgt tttgaatttt agtgtacgat
//
LOCUS       DACESX010000262         1173 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.267, whole genome shotgun sequence.
ACCESSION   DACESX010000262 DACESX010000000
VERSION     DACESX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.267"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..>1173
                     /locus_tag="IXF88_002765"
     CDS             <1..>1173
                     /locus_tag="IXF88_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAQ1509445.1"
                     /translation="GSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIGIVGVL
                     IIPLLPLFIKNSTDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAINDFAF
                     LFFMNIVQVIALYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVVSLDKQ
                     TKDEVKKNVIGNMSSKIGGVIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQNLCKQ
                     ITNSITASVGNFAVSNKKKDSFELFKKHFFINHSLIFFSSILLLTLMNPFISLWVGAK
                     YMLPMSTTILIVLNYSVQVYRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSLLLLIV
                     FDMGINGVLIGTIGSSLGFVIWYEAFIIYKTVFEKRFIHFFLTFWRSFIELIISAIV"
BASE COUNT          386 a          149 c          182 g          456 t
ORIGIN      
        1 ggctcttcta ttatttattc cctatacaaa ccattagccc aaaaggatga aagtaaagta
       61 aaggctttaa tgaatttgta taagaagaca tatgaattta ttggtatttt tatcggcata
      121 gtaggagtat tgattattcc gctgttacct ctatttatta aaaatagcac agacatacca
      181 catatgtatc gatattatgt attattttta ttaaattcag ttcttagtta ttttttcacg
      241 tataaaagat cacttgcaat agcagatcaa aaaaattatc tggttgcgat taatgatttt
      301 gcctttttat tctttatgaa cattgtacaa gtaattgctt tgtactttta ttctaatttt
      361 actctatttc taatagtgca gattttgttt actctatttt ctaatataaa tatttccaga
      421 acagttgaca agagatattc atatttaaaa gagaaagatg tagtttctct agataagcaa
      481 acaaaagatg aagtaaagaa aaatgtcata ggtaatatgt cctcaaaaat aggcggggtt
      541 attgtaatgg ggacagataa tatattaatt tctacatttg ttaatttaac agctgtagga
      601 atttattcta actattcatt gattgtcaca agtgtgcaaa atttatgtaa gcaaattact
      661 aattctatca cggcaagtgt aggaaatttt gctgtatcaa ataaaaaaaa ggattcattt
      721 gagctattta aaaaacattt ttttatcaac cactcactaa tctttttttc ttctatattg
      781 ttattgactt taatgaatcc cttcattagc ttgtgggtag gagccaaata catgctacct
      841 atgtcaacga ctattttaat tgttttaaat tatagtgtac aggtttatag aaatacatcc
      901 ttcgttttta tagagtcatt tggtttatat tggtctcaac ggaaaaagcc tataattgaa
      961 gcaggtgtaa acttgttatt atctttgctt ttgttaatag tttttgatat gggaatcaat
     1021 ggagtactta tagggactat cggatcgtct ctaggatttg tgatttggta tgaagcattt
     1081 atcatatata aaacggtatt tgagaaacgt tttattcatt tctttttaac tttctggcga
     1141 agttttatag aattaataat ttcggcaata gtc
//
LOCUS       DACESX010000263         1143 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.268, whole genome shotgun sequence.
ACCESSION   DACESX010000263 DACESX010000000
VERSION     DACESX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.268"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..144
                     /locus_tag="IXF88_002766"
     CDS             <1..144
                     /locus_tag="IXF88_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1509446.1"
                     /translation="YRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLG
                     IQA"
     gene            196..342
                     /locus_tag="IXF88_002767"
                     /pseudo
     CDS             196..342
                     /locus_tag="IXF88_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            419..748
                     /locus_tag="IXF88_002768"
     CDS             419..748
                     /locus_tag="IXF88_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1509447.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            738..1025
                     /locus_tag="IXF88_002769"
     CDS             738..1025
                     /locus_tag="IXF88_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1509448.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          415 a          153 c          220 g          355 t
ORIGIN      
        1 tatcgaagtt tacgcactgc ctctaccacg attaaaggca tggaagccat tcgaggatta
       61 tataagaaaa cccgaaaaga aggcactctc ttcgggtttt cggtctgtac tgaaatcaag
      121 gtattattgg gaatccaagc ttaaatcata gataccgtaa gggattttat tctttattta
      181 aaactttgca acagaaccca atcatttgag aagttaaaac ctacacatga aggtaggtat
      241 tttagaagat tgaatagaca acaccgggtt gtttataagg tagatgaaga tgaacacgtg
      301 gttgaaattt attcagcaag gacgcattac gagtcacagt aattacttgt aaatacatta
      361 tctcgtggta taatgtattt actaagtaat tacatactgt tagaaatgag ggatttaaat
      421 gattacaaaa acaagaaaac aaggcaattc aatcatgttg acagtaccta aagattttaa
      481 tgttcctaat ggagtcgaag ttgaagcaaa gttggtagag aatggtattc tttatgaatt
      541 tgttgaacca caaaaagaat tttatgattt tagcgaagat attttgtctg atataattgc
      601 agaaggctac gataaagatg agattcttgt agaatttaag aatagaaaaa acaaaatgca
      661 ttcttccttt agagatattg ctgaagatac acttactaat tctaaagtaa tgactaaaga
      721 ggagttggca acagaaattg gcttatgaaa tcgttccttc aaaacatgtc attaagtatt
      781 taaaaaagct aaaagagaag ccattgaaag aaaaattttt gaatttaatt tatgatgaaa
      841 ttgcgattga tccatataag ggtgaacaaa aaactgggga tttatcaggt atttggtcta
      901 gtgggttcaa atatgcgggc acaacatata gagtagctta cgagattata gaaaacaagg
      961 ttattccagt attgttatgt ggaacgcatg aaaactttta tgaacagttg aaaaaattaa
     1021 tctagtgatt tgttgtcgtt cttaagaagg acggcttctt ttttatcgaa aatctgattc
     1081 gtctacatga ctaaagtcgc gagtattctc tgcttctcga taaattaaat aacgtcatta
     1141 ctt
//
LOCUS       DACESX010000264         1133 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.269, whole genome shotgun sequence.
ACCESSION   DACESX010000264 DACESX010000000
VERSION     DACESX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.269"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(318..764)
                     /locus_tag="IXF88_002770"
     CDS             complement(318..764)
                     /locus_tag="IXF88_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1509449.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
BASE COUNT          354 a          234 c          152 g          393 t
ORIGIN      
        1 aaatgtgatt taaaagtatg ttcaagacca ctcaccagta agtttaatct atttagcaaa
       61 agaagctatg tctacactct ttaaaatcaa tatgttttct catttttaaa gtttcaaaaa
      121 aactctataa ctaagaaaaa tcacaaaaaa gagtacattt tctagtgaaa ctgcaccctt
      181 ttttgctttg tttacgtaca aaaaatctct tgagtactta acttctacag tataatcaac
      241 aaccccttta tagattactg atgttatgct gaaaaaaatt ttttcaccaa cttgtaatct
      301 aagccacttt tcttatttta cttctcaaac caatcataaa attcatctgg tgttttctcc
      361 cgatagagtt tgattaacgc tggtcgcatc gattgaaata tttcgactaa ttcttcccca
      421 ccaaatcgag tggaaaaatg cttatcagta aatatcattg ataaagaaga aagaaacagc
      481 tccgtatctt catctgtacc aagaatattg atcaactttc caatcaaata ggaatcatta
      541 aatgtcaggt cttgtttcac atcaaaggtt tttattgatt ctttcaccat tttcgcaatg
      601 cgatctttct ctccatactt gatctcacca ctcatctcga tatagtcaat gatcccagat
      661 gaaagaatcg tgacagcttc ttcttttgct tttgatgata gtttgtttag cgttttggat
      721 tcttcaagta cctctttcgt aatctgtacg acatagtcct tcatgatttc actcgaatat
      781 tctacaaaac attcttggat aaattcctcc aaaggatagc ctagctcatt ctcccaagca
      841 ggatttttga actcgcccgt ataaaattct tgctttattt tctcaatgat ttcaggataa
      901 tcgtccacta tttttttctg accaatgaga aacaaatact ctcggatata ttcttccaca
      961 tgcccaaaat aaaaccagcc gacaggtttt ccgctgatac tagataattg gttgatcaca
     1021 ctcttaggag ccaacgcata gccttgaacg taggaactga tcgtcggctt ttttaaacct
     1081 aatcgattcc ctaattctgt aaaagagaga gacattccgt cttttatttc ttt
//
LOCUS       DACESX010000265         1128 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.270, whole genome shotgun sequence.
ACCESSION   DACESX010000265 DACESX010000000
VERSION     DACESX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.270"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            118..1128
                     /locus_tag="IXF88_002771"
                     /pseudo
     CDS             118..1128
                     /locus_tag="IXF88_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209344.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          340 a          241 c          214 g          333 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaaatggca agaagatctc tatgattctc ctgaatcgta gcggtaataa acggacctat
      181 cttcggttga agaaacagcg atacctctgc cgagcatgta aaaaatattt taccgctcgg
      241 acgtatctcg ttaccccatt ctgctttatt tctaagcaga ttcattacaa gatcttagaa
      301 gaattgacag agcgtcaatc tataaaagca attggtaaac attgcgatgt ttctgtgaca
      361 actgttcagc ggacattgtc cgccttgtca tcagatatcc gtcagaatct tgagtattta
      421 cctcggtgtc tattattcga tgagtttcgt tctctttcta catggcacgg taaattttct
      481 ttttcttgta tggacggtga gaccgggaaa ctgtttgaga tcttacctag ccgccgtaaa
      541 aaagatctcg ttgctttttt catgcgtttt tctctcaaag cgaggttggg tgtacattac
      601 atcgtaacgg atatgaacgc tccttatttc tcactagtta aagagtgttt tccaaatgca
      661 caagtgatcg tcgatcggtt tcacattgtt caacatctga accgcttctt cgatgcggtt
      721 cgaaaacgtg tgatgaaaac acttgatcaa aaagacccaa tacaagctaa acagtatcgc
      781 caattgaaat ccttattcaa attactttta aaatcagagg atcgtttaga ttacaataca
      841 ctaacgaaaa gacgcaattt caattggaga ctattgactg aaacagaagt agttgaccgc
      901 cttctaaaga tttctcctga acttaaaaca gcttatcgtt actaccaaga cctgcttact
      961 gcgtatcagg aaaaagatcc agatactttt ttccagctgc ttcgagcaat gcctggagaa
     1021 gttccattgg aactgcacca tatcaaaaaa gcctttttca aatacgagaa agggattcgg
     1081 ctggcattac tgaagaagta ttcgaatgca cgactggaaa atctccat
//
LOCUS       DACESX010000266         1124 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.271, whole genome shotgun sequence.
ACCESSION   DACESX010000266 DACESX010000000
VERSION     DACESX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.271"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..>1124
                     /locus_tag="IXF88_002772"
     CDS             <1..>1124
                     /locus_tag="IXF88_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAQ1509450.1"
                     /translation="PAPHTCRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVM
                     RLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSL
                     TTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLP
                     TRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQA
                     FNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLT
                     EAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQN
                     LLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKS"
BASE COUNT          371 a          223 c          217 g          313 t
ORIGIN      
        1 cctgctcccc atacctgtag aaattgtggt tctactgtgg tagatggaaa cgggaaagta
       61 attgtagtga aaaatgggaa gaaggaaacg attgtccgct ttgaacaata caatcacatg
      121 cctttggtta tgcgcttaaa aaagcagcgc tatacctgta aaaactgtcg aacccattgg
      181 acggctcaaa gttattttgt tcaacccaga cattcaatcg cgaatcatgt cagatataaa
      241 attgcttctt tactgactga aaaagtatct ttatctttta ttgcgaaaaa ttgtcaggta
      301 tctttgacca ccgttattcg tacgttgaaa gagtttaaaa gctacttacc aaagcaatct
      361 aagaggattc tccccagagt attgatggtt gatgaatttc gttcgcacgc ttccatagaa
      421 gataaaatga gctttatttg cgcagatggc gaaacaggac aattaattga tgtcttgcct
      481 acgcgtaaat tgcctcgatt aacaagctat ttcttaggct gcgctaatcc tgaagaagta
      541 aaatttttag tcacagacat gaacgccgcc tacttccagc ttaccaaacg tattctgcca
      601 aatgcgaaag tagtgattga tcggtttcat attatcaaac acatgaatca agcctttaat
      661 gagttgcgta tccgtgaaat gaatgaactt cgtaaagtag gacagaaaag tcaggcagaa
      721 aaactgaaaa agaactggcg ctttttgcta aaaaatcgtg caaacatcaa ccattatgaa
      781 tacaaaacat ggaaaagttt tcgagcacca aaatacccat ttcttacgga agcgatgatg
      841 attgatcgat tgcttggctt ttctacgtcc ctgaaggagg cgtatcccta ttttcatgaa
      901 ttagttgaag cctttcgaga caaagaccct gacttatttt tctccttatt ggcagaactt
      961 cccgaaacgt tagatgacgg ctttcgggaa aagctccaaa accttctgac ctatgaagaa
     1021 ggcatcacca acgcgatgat ctatccttac accaatggaa aaatagaagc gaagaatacc
     1081 catataaaga caatgaaacg agtatcctac ggatttaaat catt
//
LOCUS       DACESX010000267         1113 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.272, whole genome shotgun sequence.
ACCESSION   DACESX010000267 DACESX010000000
VERSION     DACESX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.272"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..227
                     /locus_tag="IXF88_002773"
     CDS             <1..227
                     /locus_tag="IXF88_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAQ1509451.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..574
                     /locus_tag="IXF88_002774"
     CDS             227..574
                     /locus_tag="IXF88_002774"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509452.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            571..>1113
                     /locus_tag="IXF88_002775"
     CDS             571..>1113
                     /locus_tag="IXF88_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1509453.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRL"
BASE COUNT          405 a          159 c          217 g          332 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt tta
//
LOCUS       DACESX010000268         1104 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.273, whole genome shotgun sequence.
ACCESSION   DACESX010000268 DACESX010000000
VERSION     DACESX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.273"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            164..715
                     /locus_tag="IXF88_002776"
     CDS             164..715
                     /locus_tag="IXF88_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ1509454.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          413 a          158 c          212 g          321 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgaagagc gagcaagaca ggaacgagag aacaaacata ctcatgaaaa gactagaggc
      181 ttcgacctcg aactttaata ataagttaga tgtttttgaa gaaaagacta agcatgcaag
      241 attaaacttt gaaacaactg caaagcacta tattaaacga cttgatgaag ataatttgaa
      301 attagatttt caacaagcta ttcaagatga attaagcgat actaaagacg aaatacgtga
      361 agtgacgaaa caagcgagag aagaaacaaa agagtataaa gaaatcttag aaagtaaaat
      421 aaaagaccat aacaaagtgg ttgataaatc gaatacagcg ctaaaagtga tgacaaaagg
      481 cgttacaaat atattttttg tgctaattat ttttgtactt gtaatgcttg tgacaggtcc
      541 gataggtcat ttctttggta ttgaacattt atatagcttt attaatggtt ttattgatga
      601 ccacgaaagt gcatggcgat atttgatgtt gatactttat gcagtaccct atgtattctt
      661 cgctatcatt ctgtggctca tagcattgtt ttttagagta tttaatgacc tttaggtaga
      721 tttatatata aaaagggcta aatacgcaaa ataccccctt aaaatgcaaa ataaatgcaa
      781 ataagggggt aactccttat gaaaaaacaa ctaatagtat tataacacgt ttttatgccg
      841 agaaaactta ttggttggaa tgggctatgt gttagctaac ttgttagcga gttggttgga
      901 cttgaattgg gattaatccc aagaaagtac caactcaaca acacataaag ccctgtaggt
      961 tccgaccaat aaggaaattg gaataaagca acgaaaggag ttgagtaaaa tgagtgagaa
     1021 aacgaagaaa atcattcgag tatcattagc tttttcacaa gtagtagtgc aatcattgat
     1081 agtttattat acttaccagc aata
//
LOCUS       DACESX010000269         1084 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.274, whole genome shotgun sequence.
ACCESSION   DACESX010000269 DACESX010000000
VERSION     DACESX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.274"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..660
                     /gene="tnpB"
                     /locus_tag="IXF88_002777"
     CDS             <1..660
                     /gene="tnpB"
                     /locus_tag="IXF88_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1509455.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          410 a          175 c          190 g          309 t
ORIGIN      
        1 tatatttctt tgttatgcaa agaagaagtt cgcgaactac ctaaatctaa ttctgctgtt
       61 ggtattgacc taggtattat agactttgca attctttcta ccggtcaaaa atttgataat
      121 aataaattta cgtccaaaat ggaaaagaaa ctcaaacgtg aacaacgtaa gttgtcaaga
      181 cgtgccttat tagctaaaca aaaaggtatc aatctgtttg aagctagaaa ttatcaaaca
      241 caaaaacgta aagtagcacg attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat tatcaaaaat cacgatatca tctgtattga agacttaaac
      361 acaaaaggca tgttgcgtaa tcataaacta gcaaaaagta tttcggatgt ttcttggtct
      421 agttttgtgt ctaaattaca atacaaggct gactggtacg gtcggaaaat catcaaagta
      481 gataagtggt ttccatctag ccaaatctgt tcgaattgtg gacataaaga cggcaagaaa
      541 tctctcgaaa ttcgaaaatg gacttgccct atttgtcata ctcatcatga ccgagatatc
      601 aacgctagta tcaatatcct gaccgaaggt ctaaggctac actctatggg attggcttag
      661 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
      721 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaagctccc acttcaaacg
      781 taagaaaccg ttaggtttag ctaagtggtg agtagttcac taaaccaaga aaatatgaca
      841 acctatacaa ttttatacac aatagaacaa caataaataa caacagtaac actcgagttt
      901 tgattaattc agcatatttt catattaaat atttaaagaa atatgagtat gaagaagttg
      961 tattagatta tgggctaaat agacagtcat ttttaattta tgcaataaat aacggaaatt
     1021 tgccaagcaa taaaaagaac ataattattg aaacatatgc ctatctggag gggggatcta
     1081 aaaa
//
LOCUS       DACESX010000270         1077 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.275, whole genome shotgun sequence.
ACCESSION   DACESX010000270 DACESX010000000
VERSION     DACESX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.275"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(27..>1077)
                     /locus_tag="IXF88_002778"
     CDS             complement(27..>1077)
                     /locus_tag="IXF88_002778"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1509456.1"
                     /translation="IAGNWKYKSDEERKADEVKYGIKIYQVDFYRNPLNLKNIKAYHQ
                     IVEIIKNEKIDIIHCNTPIGGVVGRIAGLKCKVKKVIYQAHGFHFYSGAPKINWFIYY
                     PMERWLAHYTDVLITINHEDYTLAQKLRLRKGGKAYYIPGVGIDTNRFMSSTSIRNFR
                     ESICKELNIPDTAKIILSVGELNENKNHLSVIHALQKIPLHTDIYYIIAGVGEMRETL
                     QNIANELGLHQRVFLLGYRSDIPKLLAAANIYILPSLREGLNASLMEAMASGLPCIVG
                     NIRGNVDLIEDGKGGFLINPKDEEEIAGAINRLADNKLLCESMGAHNLDKIKDFDLSV
                     VINQINNVYHEIVSK"
BASE COUNT          332 a          203 c          131 g          411 t
ORIGIN      
        1 tcatattaat atcactttca attcaattat ttacttacaa tttcatggta aacattattt
       61 atttggttta tcacaactga taaatcaaaa tcttttatct tgtctaaatt atgtgccccc
      121 atagattcac acaataattt gttatcagcc aatctattta tcgcaccagc aatttcttct
      181 tcgtctttag gattgattag aaatccaccc ttaccatctt ctataagatc tacatttcct
      241 cttatattac cgacaataca tggtaaacca ctagccatcg cttccattaa agaggcgttg
      301 aggccttccc ttaatgaggg tagaatatat atgtttgcag cagctaataa tttgggtata
      361 tcacttctat atcctagtaa aaaaactcgc tgatgtaatc ctaattcatt agcaatattt
      421 tgtagtgtct ccctcatttc tcctacccca gcgataatat aatagatatc tgtatgcagc
      481 ggaatttttt gtaatgcatg tattactgat aaatggtttt tattctcatt tagctctcct
      541 acagataaaa tgatttttgc tgtatcaggt atattaagtt ctttacatat actttctcta
      601 aaattcctta ttgatgtgga agacataaac ctatttgtat ctatacccac acctggaata
      661 taataagctt ttccaccttt cctaagacga agtttttgag ctaaagtata atcctcatga
      721 tttattgtaa tcaaaacatc tgtataatga gctagccaac gttccatagg ataatatata
      781 aaccaattta ttttaggagc acctgaataa aaatgaaatc catgtgcctg gtaaattact
      841 tttttgactt tacatttaag accagcaatc cgtccgacaa cacctccaat aggtgtatta
      901 caatggataa tatctatttt ttcatttttt ataatctcta caatctgatg ataagctttt
      961 atatttttta aatttagtgg gtttctataa aaatcaactt ggtatatttt tattccatat
     1021 ttcacttcat cagcttttct ttcctcatcg gatttgtatt tccagttacc agctata
//
LOCUS       DACESX010000271         1063 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.276, whole genome shotgun sequence.
ACCESSION   DACESX010000271 DACESX010000000
VERSION     DACESX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.276"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(38..994)
                     /locus_tag="IXF88_002779"
     CDS             complement(38..994)
                     /locus_tag="IXF88_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509457.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caa
//
LOCUS       DACESX010000272         1035 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.277, whole genome shotgun sequence.
ACCESSION   DACESX010000272 DACESX010000000
VERSION     DACESX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.277"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..>1035)
                     /locus_tag="IXF88_002780"
     rRNA            complement(<1..>1035)
                     /locus_tag="IXF88_002780"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACESX010000273         1011 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.278, whole genome shotgun sequence.
ACCESSION   DACESX010000273 DACESX010000000
VERSION     DACESX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.278"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(227..775)
                     /gene="aac(6')"
                     /locus_tag="IXF88_002781"
     CDS             complement(227..775)
                     /gene="aac(6')"
                     /locus_tag="IXF88_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1509458.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          293 a          211 c          150 g          357 t
ORIGIN      
        1 gtttctcggt attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt
       61 tcttactact cgaaactaag cgactttttc acaacctctg gtacacggtg ttccaaaagt
      121 agcttcttcg gaaataagtt ggaatttcac aaaaattcaa gaagtaattt tcgtaaattc
      181 cttcttattt cttgaagctg attacttctg tcacaacctc tttttattat tgagaatttg
      241 gtcgaggaat aatcgttttt gccatccaaa tatccggttt gtcccagcca tttgcatttg
      301 gtaagacacc tacgatttta taacctaatt tttcatagaa ttcatacgga tgttcacgaa
      361 ggttctggat ggaagccact ttatcaaatg tatgctcata caggtccgtt tgacttaacg
      421 ttgttccatg gtctaaatca tccgtaccta aataaatcgt gattcctcct ctggaagcta
      481 cttctttttc taagtaattg actaatcgag tacctatttg gttctttcgt cgggagcttt
      541 ctacaactaa tggatgcaat tcccaacctg tgataccgta ttgagggatt gcaccaataa
      601 atcctactaa ctcatcttgg tctaccgctg ctaccgcgat tcgttctgga ttcatcattt
      661 cttctacttc ttctgccgag ctgtctccat attcttccgg ccaagtcagt ctcagtaaat
      721 cagaaagctg atctttcaat actggattat tacggtcaaa ttcactgatt atcatacgtc
      781 ttcccccttg ttcttgctta ttctaacagt ccttttgaaa aaacgaaatg ttctcgctca
      841 aaaatcttta gctaccaatc agtatctcta ttctttcatt atttatctta cttataaaat
      901 aaaaaaatta tacagaaaca ctttattcaa aataggaaag atcaggaaag ccatcaattg
      961 gaggaaaaaa ctctgtttgt tcatcatgat atagattatc ttttgcttta a
//
LOCUS       DACESX010000274         1004 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.279, whole genome shotgun sequence.
ACCESSION   DACESX010000274 DACESX010000000
VERSION     DACESX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.279"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..250
                     /locus_tag="IXF88_002782"
     CDS             <1..250
                     /locus_tag="IXF88_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1509459.1"
                     /translation="FNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQ
                     HIGDSLNGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            269..>1004
                     /locus_tag="IXF88_002783"
     CDS             269..>1004
                     /locus_tag="IXF88_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1509460.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPL"
BASE COUNT          316 a          153 c          183 g          352 t
ORIGIN      
        1 ttttaataga aataaatcta ttgaggaaaa agttctagta ctaacggcta atggaacaag
       61 ccaagactct tctgaccaag aaactattaa aaaaattgca ttagcatcaa aagcatgtgg
      121 agctgatatt caacacatag gggattcttt aaatggtatt tgtcttccag aaaatatttt
      181 tgctttgggt caagcaattc ggggatatcg tcatcaaatt attatgttag gaaaatctaa
      241 ttatcgttaa ttaaggagga aatgaaagat gatcgacaaa gttcaaaaaa tagctgcaaa
      301 aattagtaat caaagacatc tactagcttt aagagacagc atggctgcag ttatgccatt
      361 aatcattatt ggttcctttt ttatgttatt agcaaatttc ccgatagaag gatttacaaa
      421 atggttatct gaaattggag tacaatcctt tttgaacaaa gcatcagatt caacatttgg
      481 tattattgga ttagctgtta cctactcttt gggacacaat ctagcaaaga cttatggtgt
      541 agactcatta tcagcaggat tattatcttt gagtggatat gttttactta cacctttatt
      601 ggatgcagat ggttctatag gatttccaac aaaatacttt ggtacctcgg gtctttttgt
      661 tggtattatt gtagcacttg tatctacaga aattttccgg tggtttgtac ttaaaaatat
      721 tacaattaaa atgccggcaa gcgttccacc aaatgtaagc cgagctttcg ttgctattat
      781 tcctggcttt tttgtcgttc tactatggtt tattgttgtt gtaatttgtt ataaacttgg
      841 aattgaaaat gttcatgctc ttattgcaga tacactcgca aaaccactat ctttactgac
      901 gaaaacttta ccaggaatta tattggttat acttattcaa tgtttctttt ggatgtttgg
      961 tattcatggt gcacaggtaa cggggccaat aattgaaccg ttat
//
LOCUS       DACESX010000275         1001 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.280, whole genome shotgun sequence.
ACCESSION   DACESX010000275 DACESX010000000
VERSION     DACESX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.280"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..976
                     /locus_tag="IXF88_002784"
     CDS             <1..976
                     /locus_tag="IXF88_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1509461.1"
                     /translation="VFIVSDEYKKMKDSTKIAYMLLKARLEIAISKRQIDEEGNVYFT
                     YTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMGFNKQLGKNNPNRLYLAELKVSE
                     NDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAESLGAQEGMKIIPCQNVDKSEGM
                     KIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFETIMTNDSIATFIPERVLNLIKTF
                     SSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDSYYVNAEQELYTTLLKAYQKLKT
                     EKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSESFPKTITKDWLE"
BASE COUNT          417 a          153 c          167 g          264 t
ORIGIN      
        1 agtctttatt gtttctgacg aatacaaaaa aatgaaagat tcaactaaga ttgcctatat
       61 gcttttaaaa gcaagattag agatcgcaat cagcaaacgg caaatcgatg aagaaggtaa
      121 tgtttatttt acttatacga caaatgaact atgtagcgta ttaaactgcc aaaaacaaaa
      181 agcgatagca atcaaaaaag agttggaatc ctttggttta ttattacaaa agcagatggg
      241 atttaacaaa cagttaggga aaaataatcc taatagacta tatctagcag aattaaaagt
      301 ctcagaaaat gatatctact tactcgaaaa atttgataga gagaataggg aaaacgttga
      361 taaatcagag ggtatgaaaa tcatacccac cctcgacgaa aaatcagacg ctgaatccct
      421 tggggctcaa gagggtatga aaatcatacc gtgccaaaac gttgataaat cagagggtat
      481 gaaaatcata ccagaactta ataataatat attagacact aatagacaca atatagacac
      541 tgaaaaagac cgcctacaag atcaattgtt gttagacaat tttgagacaa ttatgacaaa
      601 cgacagcatt gctacgttta tccctgaacg agtattaaat ttaataaaaa cattttcttc
      661 aagttacagt gaagctcaaa aaactgtcca gactattcat aatgcaaaga aaaaagccga
      721 aacagaaagt ggtatttcaa taatttttga agaactcgat agttattatg tcaatgcaga
      781 acaagaatta tacacgacac tgttaaaagc ctatcaaaaa ttaaaaaccg aaaaagttga
      841 aaatatccag aacctgattt ttgtctatgt aaaaaattgg tttatcgaaa aaccaatagc
      901 tgctaaagta tcaagtgaaa aacgtttgaa ttctgaaagc ttcccaaaaa ctattacgaa
      961 agactggtta gagtgaaatt tcactgacaa attgattgtt g
//
LOCUS       DACESX010000276          991 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.281, whole genome shotgun sequence.
ACCESSION   DACESX010000276 DACESX010000000
VERSION     DACESX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.281"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(21..94)
                     /locus_tag="IXF88_002785"
     tRNA            complement(21..94)
                     /locus_tag="IXF88_002785"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IXF88_002786"
     tRNA            complement(108..181)
                     /locus_tag="IXF88_002786"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IXF88_002787"
     tRNA            complement(209..281)
                     /locus_tag="IXF88_002787"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IXF88_002788"
     tRNA            complement(301..374)
                     /locus_tag="IXF88_002788"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="IXF88_002789"
     tRNA            complement(384..457)
                     /locus_tag="IXF88_002789"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="IXF88_002790"
     tRNA            complement(468..553)
                     /locus_tag="IXF88_002790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="IXF88_002791"
     tRNA            complement(570..641)
                     /locus_tag="IXF88_002791"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="IXF88_002792"
     tRNA            complement(653..725)
                     /locus_tag="IXF88_002792"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="IXF88_002793"
     tRNA            complement(743..824)
                     /locus_tag="IXF88_002793"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="IXF88_002794"
     tRNA            complement(832..904)
                     /locus_tag="IXF88_002794"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="IXF88_002795"
     tRNA            complement(908..980)
                     /locus_tag="IXF88_002795"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DACESX010000277          959 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.282, whole genome shotgun sequence.
ACCESSION   DACESX010000277 DACESX010000000
VERSION     DACESX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.282"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..900)
                     /locus_tag="IXF88_002796"
     CDS             complement(<1..900)
                     /locus_tag="IXF88_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1509462.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLS"
BASE COUNT          328 a          112 c          166 g          353 t
ORIGIN      
        1 ggatagataa tactttgaag gggataaccc tgtatatttt ttaaaagctg aagaaaaatg
       61 atggatcgaa tgaaatccta atatatctgc aatttctgta taggttaatt cattttctag
      121 cacgagacgt tttgcttttt ccatttttag ttctttaaaa tagctaataa cactttttcc
      181 attcactttt ctaaaatgtt tttttaagct ggctggactg ataaaaaaaa tttgggctaa
      241 ttgttcaagc aaaaaatttt ctgaaagatg ttcttctaaa tatcgtatga ttggatcaac
      301 taattcatct gaataaagaa tatgcttttt tttaggaatg actttctcat ttgaaggtat
      361 tgtcaataaa gcaagttcct gttgataatg ccttggtaag gaagacaatt gaattttttg
      421 cagacaataa gtaactaata aggaaagcaa atgaatgctc atctgttggc atagtatgaa
      481 aaaagaattg atttcctgat cataggaaat agctggctta ctatgtttta gataatcaat
      541 tttgaactgt tcatttttca gaaaagtaat gatagatgtt atttcttgat ctatagaaaa
      601 agattgtccc gtaatatgtg ctaataattg ctcatcaaaa taaaactgga taatgataaa
      661 agtttgtgtt tccttttgag tagaaaagat ttggacttct tgctgtttat caatgaataa
      721 aagggaagga ttatctagtt ctatagattt atgtgcaaat tcagcatata aattagcgct
      781 ttcatttatt agtagtcgaa aatagggagc agcagataaa tgaagatggg tatctatgga
      841 ataggttagt gtttttttga caagattgga ggattggttg tttgttaaca aaagaaccac
      901 tttcctttct ctttttatat aaaaagcttt ctatttataa ttaatgacta ttataacaa
//
LOCUS       DACESX010000278          943 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.283, whole genome shotgun sequence.
ACCESSION   DACESX010000278 DACESX010000000
VERSION     DACESX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.283"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            2..>943
                     /locus_tag="IXF88_002797"
     rRNA            2..>943
                     /locus_tag="IXF88_002797"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACESX010000279          931 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.284, whole genome shotgun sequence.
ACCESSION   DACESX010000279 DACESX010000000
VERSION     DACESX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.284"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            85..825
                     /locus_tag="IXF88_002798"
     CDS             85..825
                     /locus_tag="IXF88_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509463.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          339 a          169 c          187 g          236 t
ORIGIN      
        1 taaaataatc agcgcattgc tactgaaaac aatgaatctg tgctattatt gagaaaagtt
       61 ccatttttga gaaggatggt ttgaatgaaa gaaatcagac gaaaacattt ccgttttcct
      121 gcgtacgatg atgagttagg cgtaaaatta accaacgaca agaagcgagt attatttgac
      181 ggacaagaag atcttttgac tgaacagaat cagtccccgt ttgaacagat gaaagaattt
      241 catttcacga acacgaacac cgagaaaatc cgtcgcaagc gtcgtgcaac aaatggattg
      301 aagtcagctc cgcgtaagga aaatttggaa aagcataaag gaaaattacc ggattatacc
      361 ggccattacc aatcagaaat cacatcgaca ggacgaaaaa aactttttgg tgattcctct
      421 cgttctgtca gacaaagtga aacaaggaca gagtccaagc atactacacg aaacgcatct
      481 gttgacacga aggtgcggga gcgttcttat tttgttccta aatatgtacc cgcatcgatt
      541 atcccagacc ctagagaaag caaaatttct gaggatcatt taatcgaatc gatgaaaaaa
      601 acggatgact cttatctgtt gttcgataca gaaccgacgc cgtatcaaga aaaaagaaat
      661 ggtgagccgt ctgtacgaac attcaatcga acaaaaacgt tagaggaaac aaagcagtca
      721 gaagaaaaaa aatcgaaaaa aagccatggt atcttagaac gctcattaca aggattgatc
      781 gatgatcaag gaaactcttt gggagaaaat agctatttta aataagtgat atacaaattt
      841 atattcggct acagttggaa aggaaacgct gcggcgtttc cttattttat actctatccg
      901 aaagtttata tcgtatcctc aaattttttt g
//
LOCUS       DACESX010000280          927 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.285, whole genome shotgun sequence.
ACCESSION   DACESX010000280 DACESX010000000
VERSION     DACESX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.285"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..348
                     /locus_tag="IXF88_002799"
     CDS             <1..348
                     /locus_tag="IXF88_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509464.1"
                     /translation="GSRGSALYKIPFRLNRVPSQLWKELFVRNWNSPPRYTTMHRPGI
                     ASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKKILLEEEQKKERENERVRSH
                     KESVQKLADELEF"
     gene            complement(345..>927)
                     /locus_tag="IXF88_002800"
     CDS             complement(345..>927)
                     /locus_tag="IXF88_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1509465.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT          292 a          158 c          176 g          301 t
ORIGIN      
        1 ggctcaaggg gttctgcatt atataaaatt ccatttagat taaatagagt accaagtcag
       61 ctttggaaag agttgtttgt tagaaattgg aattcaccac cacgatatac aacaatgcat
      121 agaccaggaa ttgcttctgt tgtagacgat gaaattttac ttaatggaac aactattgag
      181 gaagtccggg aatatcatcg ggaaacttta aaactttgca ttgaaaaaag taatactcaa
      241 gaaaaaaaaa tattgctaga ggaggaacaa aaaaaagaga gagagaatga gcgagtcaga
      301 tcacataaag aaagtgttca gaaattagca gatgaattag agttctagtt gatagtcagt
      361 tctttcttaa ttcgatacac agtttgtcta gttacattat agtctgttgc tatttttgaa
      421 atagcaacac cattctcctg gtcctgaacg atgcttctat aaacaaggcg acgttgagga
      481 tcttttgtgt tcgcactgta taacttagga cgtcctttat agacgccgtt tgctttagca
      541 atctttatcc cttgtgcctg gcgtcgtttt gattcagtac gctcttgttc agcaatcatg
      601 gccaagattt gaatgattag gtctttgata aatttatcca ggagaggatt tccgattgct
      661 tcagccatga tcggtaaatt agtgataata agtcgcacat ttttcttctt tagataattt
      721 actgaatcga taatctcgtc ataattacgt ccaagtcgat cgatagcctc tacaataaaa
      781 atatcttggt ctctaacaaa atctagaact tcctgaaaaa tcggtcgatg agtaatagtc
      841 gctccagatt gtttctcgat gaaaattttt tcagcaccaa attttctcat ttcctcgatt
      901 tgacgttgtt cattttgatc gatcgat
//
LOCUS       DACESX010000281          919 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.286, whole genome shotgun sequence.
ACCESSION   DACESX010000281 DACESX010000000
VERSION     DACESX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.286"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..904
                     /locus_tag="IXF88_002801"
     CDS             <1..904
                     /locus_tag="IXF88_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1509466.1"
                     /translation="RYIKKEKEQLKKVLKKEKPDVIIFNTFIPSVLFSEFLKYEFPTV
                     KLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEKLSLYNNHIKVIYN
                     PLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGWAIRIAGDGTEEER
                     KELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEGLPLVLIEAMTYGL
                     PVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLMEKYQKFSLERAKD
                     FNEKKIIGEWQTLLNELGNEDVRN"
BASE COUNT          368 a           99 c          159 g          293 t
ORIGIN      
        1 tagatatata aaaaaagaaa aagaacaact aaaaaaagtt ctaaagaaag aaaaacctga
       61 tgtcatcata ttcaatactt ttatacctag tgtactattc tccgaattcc taaagtatga
      121 atttccaaca gtgaaattaa ttacttggat gcactctgat cctgattaca gcttaaatca
      181 tattgctaaa ttttataaaa aagaatatag aaaaagtttt gaacgcgtgg attccattgt
      241 ttgtctttcg caagatacaa aagaaaaact ttcattatac aataaccata ttaaagttat
      301 ttataaccca ttgacattaa gcggctctag tttaataagt aaccttgata aaaaaattat
      361 ttcatttact gccagatacg atataaatat taaagggttt gattatttat gtgaagtagc
      421 taaaaactta cctgttggat gggctataag gattgctgga gatggtacag aagaagaacg
      481 taaagagctg aaaagattaa ttgagaaaaa tggtgtgtta gataaaatta tttgcgcagg
      541 tgcattattt ggagatgaat taatcaatca ttatatagaa agttctattt tcatatctac
      601 ttctagaagt gagggactac cattagtgtt gatagaagca atgacttatg gattacctgt
      661 aataagtttt gatcatggtg gtgctaagga aatattacaa gatggaaatt atggaatttt
      721 aattaagaat tttgacgtac aagatatgaa taatgagata gaaaaattgt ctaataatcg
      781 tttacttatg gaaaaatatc aaaaattctc tttagaaaga gctaaggatt ttaatgaaaa
      841 aaaaataatt ggtgaatggc aaacattgtt aaatgaatta ggaaatgagg atgttagaaa
      901 ttgaggactt cgattaaaa
//
LOCUS       DACESX010000282          913 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.287, whole genome shotgun sequence.
ACCESSION   DACESX010000282 DACESX010000000
VERSION     DACESX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.287"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..>913
                     /locus_tag="IXF88_002802"
     CDS             <1..>913
                     /locus_tag="IXF88_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1509467.1"
                     /translation="NFDYEISYNEVVTVTPRNYKLNFRKVNDLDTHYAMIGIAAGIAD
                     YPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGETKKL
                     LKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPSFDY
                     QLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNS
                     VSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDS
                     QITAIDMNKEDLDSHKLISYPIIEKQQI"
BASE COUNT          322 a          121 c          162 g          308 t
ORIGIN      
        1 gaattttgat tatgaaatat cttacaatga agtagttact gttactccaa gaaattataa
       61 gttgaatttt cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc
      121 tggtatagct gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc
      181 tgggctaaat ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt
      241 atctcctttt gaatttattc cttggatttt aggacaatgc tcaacagtag gagaaactaa
      301 aaaattgtta aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc
      361 ccctttacat tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga
      421 tggacttcat atatatgata accctgtggg cgttcttacc aataatcctt catttgacta
      481 tcaattattt aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt
      541 ttcaaatcaa ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg
      601 agatttatcc tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc
      661 tggagattca gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca
      721 acaaaaaggt ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg
      781 ttgcaatgtt gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc
      841 aattgatatg aataaagaag acttagatag tcataagtta attagttatc caattataga
      901 aaaacaacaa att
//
LOCUS       DACESX010000283          897 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.288, whole genome shotgun sequence.
ACCESSION   DACESX010000283 DACESX010000000
VERSION     DACESX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.288"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..309
                     /locus_tag="IXF88_002803"
     CDS             <1..309
                     /locus_tag="IXF88_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1509468.1"
                     /translation="LGVKGQNLLADDSFWQIEAFYLAQCAYNTTLMFSPDRIIFGGGV
                     MKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGIIGGLALARKAVRNKQP
                     "
     gene            500..652
                     /locus_tag="IXF88_002804"
     CDS             500..652
                     /locus_tag="IXF88_002804"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509469.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT          299 a          120 c          161 g          317 t
ORIGIN      
        1 ttgggtgtaa aaggccagaa tttattggct gacgattcgt tttggcagat tgaagccttt
       61 taccttgccc aatgtgcata caatacaaca ctgatgtttt cgccagatag aattattttt
      121 ggcggtggtg taatgaaaca agaacacatg aagaaaaaag ttcaggataa atttgttgag
      181 cttataaatg gatatgttga aataccacca attgatagct acattattac gcccgaatta
      241 ggtgataatg ccggaataat cggcggatta gcattggcca gaaaagcggt aagaaataag
      301 caaccataat taatgaatgt ttcattgata ctcatgtcaa aagtatttaa ccgagtaatc
      361 aaatgatcaa atgaagttgg ataaagcgaa aatgaattca aataaaaagg ctctaagtca
      421 aatcgtgttg ttttcattaa atcactgaaa ggaaatgctt ctgcgtttcc ttttttcttt
      481 ttgttgtgcg ctgtatacct tgaggatatt cactggtggc gtacacttaa atagtaatat
      541 attgtgttac tttatgtctg tggtaatagt tatagttgct taccaatgta tgttcatttt
      601 tcgaaatgtt gacttcataa tccttggctt aatgtttgaa tttttttggt gatagatttt
      661 ttcagccgta taaaataaat cgtaattcta ttatagagag ttgtgattat aaaaagatag
      721 caagaaaaaa aaagaaagtt tctatacatt ttaaatattt tattcctatt tctattgcta
      781 aaaaagtaga aattctatat ttacctttaa ctctcgcttc tcttgcaaat gtagttagca
      841 ttttaatcga taataaactc aggtagttat attaccttga aatgattgat aattttg
//
LOCUS       DACESX010000284          891 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.289, whole genome shotgun sequence.
ACCESSION   DACESX010000284 DACESX010000000
VERSION     DACESX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.289"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..>891
                     /gene="xerS"
                     /locus_tag="IXF88_002805"
     CDS             <1..>891
                     /gene="xerS"
                     /locus_tag="IXF88_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1509470.1"
                     /translation="HMPDYIKDFVQDREDKDQSPRTTLEYLKNYKLFYEWLLSESIVP
                     DTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNNNLGLSTSTINRNITA
                     LKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARANAIEKKLFLDEDTQNY
                     LDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSEVVNINLEDLSLEKNN
                     VVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKALFLAIYQGEAKRISGI
                     AIERMVAKYSKGFRVQVSPH"
BASE COUNT          354 a          146 c          147 g          244 t
ORIGIN      
        1 tcatatgccc gattatataa aagattttgt tcaagatcgt gaggataaag atcaatcacc
       61 tcgaactact ttagaatatc taaaaaatta taagctcttt tatgaatggc ttctgagtga
      121 gtctatcgtt cccgatactt caatcacctc gataagacag ttaactgcct atgatctaaa
      181 cttgtacaaa tctcatttga aacgtcgcac aaaggaaaat gtaaaaaaag agacaacaaa
      241 aactaagctc aaagataata ataacttagg tctatctact tctactatca atagaaatat
      301 cacagcatta aaggttctat ttaaatatct ttcgaaaagt tccaataatc ctttaggaaa
      361 accatactta gaaaataatc ctatggatca agtagcaact atcacagata aaactacgtt
      421 agctgcccgt gcaaacgcaa tcgagaaaaa attatttcta gacgaagata cacaaaatta
      481 tctagattat attgccaatg aatataaaaa aactttgagc aaacgagctt tgatctatta
      541 tcaacgagat gtagaaagag acctggcgat caatgcattg attttaggat ctggattaag
      601 actctccgag gttgtaaata ttaatttaga ggacctatct ttagaaaaga ataatgtggt
      661 agtaactaga aaaggaaata agagagacgc agtaaatata gcagcatttg caatggaata
      721 tttagctaat tatctagcta ttcggaaaga acgatacaaa gtaacagaaa acgaaaaagc
      781 tttatttcta gcaatttatc aaggagaagc caagcgtata tccggtattg caatcgaacg
      841 gatggtagca aaatattcaa aaggattcag agtccaagtc agtccacata a
//
LOCUS       DACESX010000285          867 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.291, whole genome shotgun sequence.
ACCESSION   DACESX010000285 DACESX010000000
VERSION     DACESX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.291"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(25..186)
                     /locus_tag="IXF88_002806"
     CDS             complement(25..186)
                     /locus_tag="IXF88_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509471.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            complement(510..842)
                     /locus_tag="IXF88_002807"
     CDS             complement(510..842)
                     /locus_tag="IXF88_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509472.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
BASE COUNT          242 a          193 c          109 g          323 t
ORIGIN      
        1 tcctctctgt cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac
       61 agaaccactt ttcgtaatat caatgtcttt attcgtatga tttttcgttt tacgatctac
      121 atgattttga agtccattta acgacccaac tttatgcttt tgtgttcgtg ccaccatata
      181 ggccataact gtgtacttcc tttcgttaaa attattgcga gaatcacaag ctaaattcgg
      241 aaaaacctcc atgtaaggta taacttacta gacttatact gcgtaaagtc tgtaagctct
      301 gccgagggct atatcggctt aacagccgat gagaaactcc gtttctctca aacgaaaaag
      361 cgaacgaaaa acgactgcct tcgcattcct tttttacgtc cttttttcgt tcaaaaccat
      421 tggctttttg cgtcattgtt ttcacaattt ttattccgca aaaaaccaaa agtgaaaagt
      481 attgcttttc acttcaaccc ctcctcaatt tagttcacat tttctgaacg aaattgtgcc
      541 ataaattgag tcaaaccctt aacatcattc gtacgccaat atgcaccatt ttcttgccaa
      601 actaatccat ctgcaaattt tcttagcgat actaaaacct catcatttac tgaatgattt
      661 cttttatttg attccaaaaa atctaatgct tcagcaggtg tcttaaagcc ttccttttta
      721 caatataatt tcgctaaaga aatcatttct ttctgtctaa tctgttttcg tttcgctcta
      781 atcttcgctt cttttgcttt caaatccgcc atttcttttg ccaatatttc tctattgttc
      841 attttgattc ctcctaaaaa taaggca
//
LOCUS       DACESX010000286          817 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.293, whole genome shotgun sequence.
ACCESSION   DACESX010000286 DACESX010000000
VERSION     DACESX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.293"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
BASE COUNT          274 a          131 c          103 g          309 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaa
//
LOCUS       DACESX010000287          810 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.294, whole genome shotgun sequence.
ACCESSION   DACESX010000287 DACESX010000000
VERSION     DACESX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.294"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            413..496
                     /locus_tag="IXF88_002808"
     tRNA            413..496
                     /locus_tag="IXF88_002808"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:447..449,aa:Leu,seq:cag)
BASE COUNT          337 a          107 c          156 g          210 t
ORIGIN      
        1 aatacaaata tgaattacaa taaaaaaatt atttgaagtt cgttcattcg ttacttatga
       61 tctctagaag aaagaaaacg tgaattactt cagcgtctag aattccagaa ggatagtaat
      121 caacagaaga aataatcaat atccaaatga aagaagatag ctgaatcgag aaaaagagga
      181 aaaaagaaat gaagaaaaaa ataaaatagt tactaatccc agagtcgttc gtttaacatt
      241 gaaatcgatc atatggataa aacagatttt ttcaccaacc ctcaatagat gatggagttg
      301 ctcggatgaa ttatttgtgt agatggcata agggagggcg tacagaaatg aaaaagaaca
      361 aaaatatgtt cttgacaaaa aacacatgta atcctaaact aaaagatgtg atgcggctat
      421 ggcggaattg gcagacgcgc tagcttcagg cgctagtgag ggtgacctcg tggaagttcg
      481 agtcttctta gccgcataat ttgttttttt ataaagagaa ggtacacctg gagcgatgag
      541 aaagaaaata acatattatc taaaagaata taggaaaaat tttaaacaac ggaaccagat
      601 tcaaataaat caaaaaaaat ttgtgtgaag gtgggacagt ttaaaaccta atagattgat
      661 tcaaatagta gatttataga ttctgataca agaaatactg gcaaacgata gttaaggaat
      721 aaagactaaa cttaaactca aaaagaggtc atgcgaaaga ataaatcgca tgacctctta
      781 ctatattttg aatgtgttta aacgttcaaa
//
LOCUS       DACESX010000288          805 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.295, whole genome shotgun sequence.
ACCESSION   DACESX010000288 DACESX010000000
VERSION     DACESX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.295"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            59..>805
                     /locus_tag="IXF88_002809"
     CDS             59..>805
                     /locus_tag="IXF88_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1509473.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSA"
BASE COUNT          290 a          162 c          122 g          231 t
ORIGIN      
        1 catgtattcc aattccctat ccgttataat aaaagaaaca ggcaacagga ggaaaatcat
       61 gatcaaatat tttacattag ataatgagaa actgcgtaat accggaaagg aaactgacca
      121 aacgatctgg tattgtgtag aacgtcctaa tcaagaagag attcatcagc tgacacatca
      181 atttgaaatt ccgtcagatt acatcacttc catattggac gatgcagaaa attcccgtac
      241 agaagaattc aatcaatcaa agttgacaaa gccagcgtta ttattgttac aatatccttt
      301 tgctaaaaca agtcctagcg gttattttca agtggataca tacccgctct ctattatttt
      361 gacgcccaaa aagaagctta tcacaattac gaatcatgaa ccactgtttt taaagaaagt
      421 ctttagccag acctttccaa aaagtgatat ttcgcctaat ctcaatatct tctttcaatt
      481 catttggcag gtcacactct cttacaacag ttatttagct acgcttcaat ctcaatgcga
      541 tactttggaa gccaaacttc aagtatccac tgaaaacaaa caactttatc aaatcatgga
      601 tatccaaaaa agcctcgttt atttcagaga agcgacttct gctaatttag atgcattgaa
      661 agaactagcg caaaataaac ttttccaaga aaatcatgct ttgaaaaatc atttacgtga
      721 tgtattggtg gaaacagagc aggcaatgac tacttctcgc attcaattaa aactagtaga
      781 acaaatgaat cagacttttt cagca
//
LOCUS       DACESX010000289          802 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.296, whole genome shotgun sequence.
ACCESSION   DACESX010000289 DACESX010000000
VERSION     DACESX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.296"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            238..>802
                     /locus_tag="IXF88_002810"
     CDS             238..>802
                     /locus_tag="IXF88_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1509474.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDI"
BASE COUNT          250 a          114 c          161 g          277 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cg
//
LOCUS       DACESX010000290          791 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.297, whole genome shotgun sequence.
ACCESSION   DACESX010000290 DACESX010000000
VERSION     DACESX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.297"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            24..716
                     /locus_tag="IXF88_002811"
     CDS             24..716
                     /locus_tag="IXF88_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1509475.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          199 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt t
//
LOCUS       DACESX010000291          771 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.298, whole genome shotgun sequence.
ACCESSION   DACESX010000291 DACESX010000000
VERSION     DACESX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.298"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..200
                     /locus_tag="IXF88_002812"
     CDS             <1..200
                     /locus_tag="IXF88_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509476.1"
                     /translation="LDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNEL
                     ERIGTIGIFEKNTSEEKNLKL"
     gene            356..730
                     /locus_tag="IXF88_002813"
     CDS             356..730
                     /locus_tag="IXF88_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ1509477.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDVEWQHFF"
BASE COUNT          314 a           82 c          160 g          215 t
ORIGIN      
        1 tgttagatga tcaaagtcct atagataaag aactaaagta tgctattttg acatttgaga
       61 aaatgacaga agaacaaaag aatgcagtta tagaaaagat gggcgatgga tataaaaatt
      121 ggcaaaatga gcttgaaaga attgggacta ttggtatatt tgaaaaaaat acatcagaag
      181 aaaaaaatct aaaattataa aatgtgatac tcatactaaa ataggagttt caagtgatgg
      241 aaaatacgat tgctatttaa gcacaaatag cggagctgaa agtaatcttc tggatgaatt
      301 aaaaagaact gagattcaaa ttatagataa aaaagaacgt cctaaaaatg gttttgtgct
      361 atcagaaaaa actgatttag aaaatacaga agcatttaac aaagaatcgg ttaaagattt
      421 acgtaaacta ccaacaaagg acataaatgg caaggatttt acaagtaaag attttgagaa
      481 gtatgatctt actatggtaa atgtatttgc aacttggtgt acagcttgtg taaaggaaat
      541 tcctgatttg gttgaggttc agaatgaaat gaaaagcaag ggtgtaaata tcgttggagt
      601 tgttacagat cctgttgatg ataacggtga aaacaaggaa gcaattgaaa agtcaaagtt
      661 aatacatgaa aagacaaagg cttcatatcc tttcttaatg ccagatgtgg aatggcaaca
      721 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DACESX010000292          767 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.299, whole genome shotgun sequence.
ACCESSION   DACESX010000292 DACESX010000000
VERSION     DACESX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.299"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(192..563)
                     /locus_tag="IXF88_002814"
     CDS             complement(192..563)
                     /locus_tag="IXF88_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1509478.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT          250 a          143 c           93 g          281 t
ORIGIN      
        1 atacactgct atatccttag tgaaatatag cagtgtatct ttgacttttt cattctttta
       61 accatatgac gcgttcatct ctatctataa ttaacattaa aaatagtgtt ttcacattat
      121 ttctcaaacc aatcttaaaa atcatcagga gtactttttt gatatacttt tattaatgct
      181 gggcgtaatg gttaaaagat ttccactagt tcctctccac caagctgagt tgaaaaatgc
      241 ttatctgtaa acaacataga caaagaggaa ataaacgttt ccgtatcttt ctctgtccca
      301 aggatattaa ttaattttcc tactaggtaa gtatcattaa acttaagatc tgccttcaca
      361 tcaaaagttt tgataaaagc ttttatcatg cgtacaattt tgtctttatc gccataattg
      421 atttcaccac tcatatcaat atagtcacta atccctgaag aaataatggc aactgcttct
      481 tctttcgcct tatcagataa ttcatttaat gactgggatt gattgatgac ttcttgagtt
      541 ttatctgtta cgtaatctct cattatccta tcggcatact ccgcaaagta atcttgaaaa
      601 aattcttcta cagggtaacc aacttcgttt tcccaaccag gagaaatttc attatcagta
      661 cttattaaat ctattaaaaa ccaacccgtt ctctgagtgt tgattctata atatttttaa
      721 tttcctatat aaatcaaaaa tattatagaa tcaataagct aattaag
//
LOCUS       DACESX010000293          753 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.300, whole genome shotgun sequence.
ACCESSION   DACESX010000293 DACESX010000000
VERSION     DACESX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.300"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            535..>753
                     /locus_tag="IXF88_002815"
     CDS             535..>753
                     /locus_tag="IXF88_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAQ1509479.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFK"
BASE COUNT          298 a           92 c          136 g          227 t
ORIGIN      
        1 tctgaatcac catttaatta cgcaaaaaat catatccata aatctcttac atatataagc
       61 gagaaggaaa atcaagatta taatactgta gtgagagaag caattagtgc tgtcgaaagt
      121 tgtcttatcg aattttcagg attaccagct aataaaaaaa tactttggga actgctataa
      181 aagcaattaa agacaaatcg aataacaatg tcgatcttac atttttaaaa ccatttgaga
      241 caatgtatgg tttggcgagt aataatggaa tacgacatgc agaaaatgaa aaaacaattg
      301 tctcagattt acctgatgca gttttggtgt taacaacatg ttcagccgta attaattatt
      361 tgggagtaaa aatagcatat gactccaata aatcatgaaa agttaaaatt catccaagag
      421 ctacattata actattaatt tccaactctt taaaagttta gtaattgaag gaagtaataa
      481 gggtgattga acaatacgga tatacggtgt atacttatcg taggagttga tgatatgaat
      541 attaaaaaac gcaaaatcag agaagtaggt aattcaatag ttgtaacttt gtcaaaagag
      601 agtttattgc aaaaaggttt gaaacctgga gatactattt ttattgatga agataaaatg
      661 atggaagcta ttgtaaaaga ggaaagcaag atagatcttg aaattgatat gtatgttaac
      721 caagctttat ctgagtatga tgcagctttc aag
//
LOCUS       DACESX010000294          753 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.301, whole genome shotgun sequence.
ACCESSION   DACESX010000294 DACESX010000000
VERSION     DACESX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.301"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(308..607)
                     /locus_tag="IXF88_002816"
     CDS             complement(308..607)
                     /locus_tag="IXF88_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1509480.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          235 a          142 c          106 g          270 t
ORIGIN      
        1 tctgaggaga gacatcaaga gtcgctgcgc tactgctcct gacctctctc ctcagaaaga
       61 aaggcatggc agttaggttc acaacgcaaa acattgtgct tttgatgagg ttgaactttt
      121 acacaagatt gtggacttga cttatactag ctgtttttgt gttaattaaa ggataaaata
      181 caatataaag aaacatctgt ttctttattt agaataaaaa aatacagact ctgttcaaat
      241 aaatgaacct agcctgcatt atgagattga ctatactttt taattcattg agatgaataa
      301 ctcgttttta taaatgccca aaagtaatcg gtgccgaagc cattcctcga cctaattctt
      361 tttcaaattt tcctattgtt gatatctcca tataaaacgg tgatactggt ttagacaatt
      421 ctcccctgat tgctaacgga cgtaaactac gttccaaatt atttatgact ttaggataat
      481 actgaccttt ttctaagcct gtctttgcgt ctatcaaaag tatacgttcc ttttctttga
      541 tctctggatt taaaacgaga tcatataatt ttagtaccat gttttctatt tcttcacttt
      601 tagacatcac gatttcctcc caaaaatata tttcttccca ctacattcta taaacaaagt
      661 atacgctaca tctagatttt tttaaatcat tactggattg ttaaaacctt tcaatacact
      721 gttatggcaa cgatttttat tttaaaaaca ttc
//
LOCUS       DACESX010000295          748 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.302, whole genome shotgun sequence.
ACCESSION   DACESX010000295 DACESX010000000
VERSION     DACESX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.302"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(18..90)
                     /locus_tag="IXF88_002817"
     tRNA            complement(18..90)
                     /locus_tag="IXF88_002817"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:His,seq:gtg)
     gene            complement(93..166)
                     /locus_tag="IXF88_002818"
     tRNA            complement(93..166)
                     /locus_tag="IXF88_002818"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Trp,seq:cca)
     gene            complement(173..253)
                     /locus_tag="IXF88_002819"
     tRNA            complement(173..253)
                     /locus_tag="IXF88_002819"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(217..219),aa:Tyr,seq:gta)
     gene            complement(255..330)
                     /locus_tag="IXF88_002820"
     tRNA            complement(255..330)
                     /locus_tag="IXF88_002820"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(295..297),aa:Phe,seq:gaa)
     gene            complement(366..438)
                     /locus_tag="IXF88_002821"
     tRNA            complement(366..438)
                     /locus_tag="IXF88_002821"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(403..405),aa:Asp,seq:gtc)
     gene            complement(448..520)
                     /locus_tag="IXF88_002822"
     tRNA            complement(448..520)
                     /locus_tag="IXF88_002822"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(485..487),aa:Val,seq:tac)
     gene            complement(536..607)
                     /locus_tag="IXF88_002823"
     tRNA            complement(536..607)
                     /locus_tag="IXF88_002823"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Glu,seq:ttc)
     gene            complement(622..711)
                     /locus_tag="IXF88_002824"
     tRNA            complement(622..711)
                     /locus_tag="IXF88_002824"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Ser,seq:gga)
BASE COUNT          208 a          205 c          173 g          162 t
ORIGIN      
        1 accccaagaa taaaaagggc gaatgatggg aatcgaaccc acgaatgccg gagccacaat
       61 ccggtgcgtt aaccacttcg ccacattcgc catggcaggg gcagcaggaa ttgaacccac
      121 actaacggtt ttggagaccg ctgttctacc tttaaactat gcccctataa aatggaggag
      181 agtggattcg aaccactgaa ccctaaggaa cggatttaca gtccgtcgcg tttagccact
      241 tcgctactcc tccatggtgg cgcgggacag aatcgaactg ccgacacacg gagcttcaat
      301 ccgttgctct accaactgag ctaccgcgcc atattttaaa attattaatt gaataacaag
      361 aacgacggtt ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca ggcatgttaa
      421 cccctacacc acggaaccag ttcattatgg aggttaacgg gatcgaaccg ctgaccctct
      481 gcttgtaagg cagatgctct cccagctgag ctaaacctcc aacattcaat taaaatgacc
      541 cgtacgggat tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac cgcttgacca
      601 acgggccagg taaatttgct acggagagta agggattcga acccttgaga cagtttggca
      661 ccatctacat gatttccaat catgctcctt cggcctctcg gacaactctc cagagtgaaa
      721 gaagctcaat actctttctc aaaatgag
//
LOCUS       DACESX010000296          748 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.303, whole genome shotgun sequence.
ACCESSION   DACESX010000296 DACESX010000000
VERSION     DACESX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.303"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            115..699
                     /locus_tag="IXF88_002825"
     CDS             115..699
                     /locus_tag="IXF88_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1509481.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          244 a          108 c          161 g          235 t
ORIGIN      
        1 atttttcaaa aaaacaattc atttcatgtt cataaaccca taacaatatc tatgttcagt
       61 taaactatat ggataaagtt atgttacaat aaaattaatg aaggagtgat ttttttgaaa
      121 ataggatacg cccgggtatc tactggttta caaaatttgg atttacaaga agacagccta
      181 aataaatttg gttgtgaaaa aatttttact gatcatatga gtggggcaaa aagtaatcga
      241 ccaggattag aaatggccat tgattttgtt cgctctggcg atacacttgt cgtttggcgc
      301 ttagatcgtt taggtagaaa tatggaagat ttgatttcaa tcgtcaatcg actgaatgag
      361 cgtggtgtga gttttcatag tattcaagaa aatattacga tggacaaatc aagttctaca
      421 ggacagctca tgtttcattt gtttgcggca tttgcagaat ttgagcgtaa tttgatcctg
      481 gaacgatccg cagcaggacg agcagctgct cgtgcaagag gacggtttgg tggtcgtcct
      541 gaaaaattgt ctgaacaaga tttggaatta ctgaagactt tagtggatag cggtacacct
      601 attaagacta ttgcggaacg gtggaacgtc tcaagaacca ccatttatcg ttatttggca
      661 aaaatggagg agaaaaatgg aaagacaaat tctcattagt ttttgtcatt taatgctgaa
      721 aatttttgcc atttatctag aaaagtga
//
LOCUS       DACESX010000297          728 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.304, whole genome shotgun sequence.
ACCESSION   DACESX010000297 DACESX010000000
VERSION     DACESX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.304"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..>728)
                     /locus_tag="IXF88_002826"
     CDS             complement(<1..>728)
                     /locus_tag="IXF88_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1509482.1"
                     /translation="VGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKI
                     FEYAIHEQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATAN
                     NGYLFLDEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLL
                     DTFDRRIQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPF
                     SGNIGKLKNIIKISCADVYSRTQE"
BASE COUNT          220 a          157 c          125 g          226 t
ORIGIN      
        1 tcttcttgtg ttcgactata cacatccgca caactgattt taatgatatt cttcagctta
       61 ccaatattcc cactaaaggg atgagataac attaaagaca aggcttctct tgaaacaata
      121 atatcttttt gtaaaacatc cgcttcattt tgaaaaaata attgaatcag ctctaatcgt
      181 tctcgaatgg ggcgatcttc aaaagttggc aacataacac taatttgaat acgccgatca
      241 aaggtgtcta ataaataatt ttcacctttt tccgtcgtgg cgaaaataaa acgaacctta
      301 gcagaatgcc aatttacatt ttcaccgact ggacgatagt ttccagtatc aataaataga
      361 aagagctttt cttgattttc tttcgatagg cgatggacct catcgagaaa aagatagccg
      421 ttgttggccg ttgccaataa accatcggta tccttttcag cccccgtaaa ggcccccttc
      481 ttgtatccaa acagtgttgc cgaaagcagt tcgggattgt ccgcataatc ggcacagttt
      541 aagatagcaa atggtgcatc tttttcaata atttgctcat ggatagcgta ttcaaaaatt
      601 tttgtggcta aaaaactttt tccaacaccc gtggctccat ttattaatac actgagtccg
      661 ttaggcggat atttgacagc ggctatacat ttttgaatga cctctctcag acttccgtca
      721 tgaccaac
//
LOCUS       DACESX010000298          715 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.305, whole genome shotgun sequence.
ACCESSION   DACESX010000298 DACESX010000000
VERSION     DACESX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.305"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            115..>715
                     /locus_tag="IXF88_002827"
     CDS             115..>715
                     /locus_tag="IXF88_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1509483.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQK"
BASE COUNT          289 a           66 c          136 g          224 t
ORIGIN      
        1 tattattgct ctgagcttta gagtagattg gtataggaaa ttggttgaac aaaaaaataa
       61 aaaaaattag aatgtttaaa ttagtaaata aaagaaaagg aattgaaaac taaaatgtgc
      121 agaataagta taatagttcc agtttataat gttgaaaaat atttaaaaaa gtgtgtcgat
      181 tcaattctag gccaaacatt taccgatttt gaattattat taattgatga tggttctaca
      241 gataattcag gaagtatatg cgatgaactt gcgaaaacag acaaccgaat aaaagtgata
      301 cataaggaaa atgggggtct aagtgatgcc agaaatattg gtattgaggt agcaaaggga
      361 gattttattg ggtttataga tagtgatgat tacattgatg aagatatgta tgcattttta
      421 tataacaata tattgaaata tgatgcagag ttatcaatgt gtggtattta tgacgtccat
      481 aagaataaag aaattaaaaa gttaactcct ttttctcaat tggttactaa atctgaagca
      541 attgaattag tattagatgg taagctagtt gttgctaatg cagtaagcaa gctatataaa
      601 aaagaattat ttgagaatgt gagatatcca aaaggaaaaa ttgctgaaga cgcagcagta
      661 atactgaaaa ttatcaatca gtgtaataaa atccatgttg atacttctca aaaat
//
LOCUS       DACESX010000299          692 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.306, whole genome shotgun sequence.
ACCESSION   DACESX010000299 DACESX010000000
VERSION     DACESX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.306"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            22..228
                     /locus_tag="IXF88_002828"
     CDS             22..228
                     /locus_tag="IXF88_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509484.1"
                     /translation="MSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISI
                     IGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(319..684)
                     /locus_tag="IXF88_002829"
     CDS             complement(319..684)
                     /locus_tag="IXF88_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509485.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          263 a           92 c          125 g          212 t
ORIGIN      
        1 ggttttagaa ctaaacgaag tttgtcttct aaaaaaaatt gggtatactc acaaactatt
       61 ttttatggag gaattgtttt aattagttta ctttcttcaa cactttattc tttgaatatt
      121 atagatgttt ccacctcaaa ttcaatatct attattggaa tcattattgc tgcaattatt
      181 acacaactgt ttttagtgtt tggagagaag aaaagaagta aaaaatagaa aaactagtga
      241 tagttttggt gatataagaa aaacatatat gtataaaaag aagaaagtag cttctctttg
      301 tacatatatg gtttgttttt atggatgccg agctttttta taagataagg ctaccgccca
      361 taacgaccca aggatttgtg gaactaagta aatacctgca agaagcaaga tcagctgggt
      421 cggttttaaa ggatgataga agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga
      481 agtgacagta gatataatat tggcaatgaa taaagatata atacgcctgt tcctttgaaa
      541 ataatatcca gcaaaaagag ataaaaatat caatgtgatg aacccgctta cggaatgata
      601 acgaaaatca agcagtaagg cgaaatgcac ataaggaaca aaagataaaa gcaacggaag
      661 gtttttccag caagttttta tcatctttca tc
//
LOCUS       DACESX010000300          685 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.307, whole genome shotgun sequence.
ACCESSION   DACESX010000300 DACESX010000000
VERSION     DACESX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.307"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(81..635)
                     /locus_tag="IXF88_002830"
     CDS             complement(81..635)
                     /locus_tag="IXF88_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1509486.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          204 a          142 c           69 g          270 t
ORIGIN      
        1 cttccgaaaa ttcagttatc ttcattaatc tcaccttcta agttttcatt atacacaaaa
       61 aatatatcct aatatctcaa ttagcctccc aatttaaaac cacgtaacga tgcccctatt
      121 tcatcttcag ttataccaat gtaccttttt gtaattgctt gcgagctatg attaaaaata
      181 aacattaagg tagcaatgtc tctagttttc ttgtagtagt gatacccaaa agtttttctt
      241 aatgtatgtg ttcctatatc atctcttcct aatttttctg ctacatctgc atatatctga
      301 tagacacgat cccttgttat atgtcgacct tgttgccgac ttggaaataa ccaatcattt
      361 tcttctttac cttcagtata taataaaatc tcttctctca aattatctat aaaaatcggt
      421 tttcgcttac ctgttttatg ttcaacaata ataggcttac ttttacctcg tatgtctcca
      481 acttttaatc ctataatatc tgacatacgt aacccattat tgattccaaa caaaaataaa
      541 aaactatctc tttctggatt acttgttctt cttagccaaa actgaaaatc ttgtatctct
      601 tcatttgtcc ttaacggttg tacattatag ctcactattg tttcccttct taaaaatatt
      661 ctgagttcat gattatatat atatt
//
LOCUS       DACESX010000301          672 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.308, whole genome shotgun sequence.
ACCESSION   DACESX010000301 DACESX010000000
VERSION     DACESX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.308"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            102..>672
                     /locus_tag="IXF88_002831"
     CDS             102..>672
                     /locus_tag="IXF88_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1509487.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSH"
BASE COUNT          209 a          131 c          128 g          204 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      361 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      421 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      481 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      541 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      601 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      661 gacaaagcca tg
//
LOCUS       DACESX010000302          671 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.309, whole genome shotgun sequence.
ACCESSION   DACESX010000302 DACESX010000000
VERSION     DACESX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.309"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..>671
                     /locus_tag="IXF88_002832"
     CDS             <1..>671
                     /locus_tag="IXF88_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1509488.1"
                     /translation="RRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQ
                     LIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNY
                     EKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRD
                     ISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNF
                     KLRIM"
BASE COUNT          213 a          137 c          128 g          193 t
ORIGIN      
        1 tcgacgcttg ccttatttag aacgctattt ttctcggttt tcattagcga ctcgtgaagc
       61 cgttcaattg attgttattg atatgtatgc gccttatgtt tcgttggtga aaaagctttt
      121 cccgaatgct cagttgatca tcgatcgttt ccacatcgtc caacatattg gcagaacttt
      181 cttgaatcat cgagtgaaag aaaccacggt acgacttaaa ggaaattccc actctcaacg
      241 agggttaggg aaaaaattga agcgctattg gaaactccta caaaaagaag aagcaaagtt
      301 aaactatgaa aaacgggttt ggcgtgccag ttttaaagat tatttgactg aaagtgagat
      361 tgccgatcga ctgttagctg cttgtcccaa cttacgccaa ggctatcagt tataccaaga
      421 cattctttat gcggtaaaga aaagagatca gtcactattt gaggattgtt taactagaga
      481 cattagcggt ctgccagaaa cctatacaac cactttacga acgtttaaaa agtttctgcc
      541 acagatccaa aatgcgcttc actacagcta ttcaaacgga ccattggaat gcttaaacaa
      601 ccacatcaaa gtattaaaac gcaatgcgta cggctttcgc aacttttaca atttcaaact
      661 acgaattatg a
//
LOCUS       DACESX010000303          667 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.310, whole genome shotgun sequence.
ACCESSION   DACESX010000303 DACESX010000000
VERSION     DACESX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.310"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
BASE COUNT          211 a          128 c           94 g          234 t
ORIGIN      
        1 aaaggtacgg gttactgatt acttgtaaat ctttacgttc cagttttatt ctttctaaaa
       61 aatatactca cttcttttct tagacttctt tttttgattc tcctctttat ggtcttgtcc
      121 ttttttgcct tcaaaatatt taaataaacc cctttcaaaa gcatttctag gttttggaca
      181 ataaggaatg acttttttct tcatataaat acctcattta actatttgat atcatcacat
      241 tatcaaaaaa aataggtttt ttattaaaac tatcttgacc tttcgggaga tttcactgtg
      301 atttattttt attcccatac ttttttcgtt ggtcactttc gtatacctaa tctaagcgat
      361 catcggcgac ggtgctttta caacgaatgg caggacaaaa caaggggcgg aaaaactttt
      421 ttgcctattt cataaaaaac tttttcctac agcaatccct tgttttgtcc taccacacgt
      481 tgtgaaccca aagcacgccg acgaacgctt agctatacga aaatgacctc caaaaacaaa
      541 aaagtgaggt gaatagaaac aaaaagaaat tgaaatctcc caaaatgtcg tccgcctcgg
      601 agacaacttg ctgctgaaaa tggttggtta attcatttaa gtattaaata agataaatca
      661 tcaaatt
//
LOCUS       DACESX010000304          648 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.311, whole genome shotgun sequence.
ACCESSION   DACESX010000304 DACESX010000000
VERSION     DACESX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.311"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(5..580)
                     /locus_tag="IXF88_002833"
     CDS             complement(5..580)
                     /locus_tag="IXF88_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1509489.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          212 a          142 c          117 g          177 t
ORIGIN      
        1 ttattcagcc tatttttctt tttgaatata tcaagcgttg gggcaacata gtgatgacta
       61 atgaaacaac gacgatagac aacaagcgat caaggtagtc tgtacccgcc tgtaccaaga
      121 tcacacttgt tgtctgactt aaacctaacc catgtaatag ctgaacgatc atactggatc
      181 ctgaggaggt gatcccgcca aataactgaa cggtaatgat cgaagaaacg atcgttccag
      241 gtaccgacaa aatcaaagcg agtaataaca gtttcaagct gtttttatct ttgccgagat
      301 atcttggcaa aatcaatcca gcaagcaagc ctgtgagaat ttggattggc gaataataca
      361 gagaaaaaat atctgtagtc actccactta aaataccgct ggacaatcct gtcagcattc
      421 caggtacagg cccaagcaat gcaccagcaa aaatcgttcc gatagaatcg agatagattg
      481 gcaaacgaag ccataaagca atcgttcctc caatataatt gatcccaata cacaaagcca
      541 tcactgtaag tacgcgagtt gccattttgt ttttattcat tttgttagcc catctttaac
      601 ctttccaaat ctttcataat cgtttcttct tgtgcattta aaagaacg
//
LOCUS       DACESX010000305          640 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.312, whole genome shotgun sequence.
ACCESSION   DACESX010000305 DACESX010000000
VERSION     DACESX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.312"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..>640
                     /locus_tag="IXF88_002834"
     CDS             <1..>640
                     /locus_tag="IXF88_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAQ1509490.1"
                     /translation="FPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSP
                     LCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVK
                     VLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQ
                     RKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL"
BASE COUNT          223 a          118 c          111 g          188 t
ORIGIN      
        1 ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat
       61 tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag
      121 ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat
      181 agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat
      241 gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga
      301 cgtgaaagtg ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa
      361 aggatatatt agccataaaa tccatgaaaa gcttgccctt aagggcatta ctatatccgt
      421 tccgccacgt aaaaacatgg ataaatcaga aaaactagac cacagcttac ttggaaaaca
      481 aagaaaaaca gttgagactg ttttttcttc cttagaaaaa ttaggttgtc aaaatttcaa
      541 ctcacgttct gttaaagggc tagagagtag atttgaaagc atattactgg cttacagtgt
      601 tctattaagt cgagcacaac gacgttttga aggaactttg
//
LOCUS       DACESX010000306          608 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.313, whole genome shotgun sequence.
ACCESSION   DACESX010000306 DACESX010000000
VERSION     DACESX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.313"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(46..525)
                     /locus_tag="IXF88_002835"
     CDS             complement(46..525)
                     /locus_tag="IXF88_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1509491.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
BASE COUNT          178 a          126 c          114 g          190 t
ORIGIN      
        1 gaaaataaaa aaacacgcat tccatattag gaatacgtgc tctgattaaa aaagtgatac
       61 ttcagttgga tggtgaagaa attcttctac tcgttttgcg tcctcctcat tttccgagat
      121 gaccatgatc gtatcgaatc cagcaattgt actgacaata gagggaggtt tgatctcatc
      181 taaaacagat gcaaataaat gagcattgtc tggcaaggta ttgacgatcg tcaaaaagtg
      241 gactcgttcg actttcaata caatgtcttc aatcatttga accagccgat cctcttcctc
      301 agtcctagtc tcaggagttc ctttttcgcg aaacaatacg aagcgggaaa cgccattttc
      361 atcaggtgcc ttcacgattt ttagatcacg gatatctcga gaaatcgttg cctgcgtggc
      421 ggtaacgcca taatgcccaa gtttgtttaa tagttcttct tgcgtacgga tcgggaattc
      481 ttcaatgact tgtttaatca gcaaatgtct ttgagctttg cgcattttct ttctccttta
      541 cgaaagattt cacatgtatc ataacaaaaa aaagaaggat tgcccaataa tattcgtctt
      601 ttatacct
//
LOCUS       DACESX010000307          600 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.314, whole genome shotgun sequence.
ACCESSION   DACESX010000307 DACESX010000000
VERSION     DACESX010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.314"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..588)
                     /locus_tag="IXF88_002836"
     CDS             complement(<1..588)
                     /locus_tag="IXF88_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1509492.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKT"
BASE COUNT          188 a          119 c           73 g          220 t
ORIGIN      
        1 tgttttaata aatctttttg ctatatctaa gttcataatt ggttcatcaa caccagcacc
       61 atgcattttt ccagcgataa ttaatccatt aaaaattgat ttggataatg ttattgcttt
      121 ctcaatagtt ctattagata ctcctgttcc tggatttcca gttaaacaaa taaaattaaa
      181 gcctaattca tttgcctttt ttaaagtctc aggagaaacg attctaccac ttgaaatttc
      241 agttcgattt tcactcatca aaacttcttt gtctacaggt tctaaattta caccaatagc
      301 tcttcctaaa gcttttttaa tccagattat tggattctct tttagagtta aaggtacacc
      361 tgaaatttga ggattaaaaa gatctagtgc attaaacaaa attaaatcag ctccagcagc
      421 tctctccaat tctgcatttg tcactgattt tcccatgtaa ggacagctac ttggcacatt
      481 ttctgccata attgttctac cttcagaagc caaaattgcc tgctttaatt ccatcggctt
      541 agctttcaaa aaatctgaag ctgaataatc taacaatctt ttattcatat aaaatatcct
//
LOCUS       DACESX010000308          594 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.315, whole genome shotgun sequence.
ACCESSION   DACESX010000308 DACESX010000000
VERSION     DACESX010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.315"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..587)
                     /locus_tag="IXF88_002837"
     CDS             complement(<1..587)
                     /locus_tag="IXF88_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509493.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVF"
BASE COUNT          263 a           88 c          116 g          127 t
ORIGIN      
        1 ataaatacac ctagactgac gatgaagagc atatccaaca gatttcctgc gacttggatc
       61 gtcgaagggt tggtcagtgg cattaaagca aggacaaaaa aggaaaacaa gctgtaaata
      121 aaacaggtga caggaactaa tcgaaagaag agcggacatt tcaattggct aaaaatgagt
      181 gaaaaaataa aataagtaaa aatggataaa atcacgacta aaccattcat gataaagaag
      241 aaaagcgtat tgtgattaaa cgaataaaaa agaattgcta aaaaagaaaa cgttttaaat
      301 gcaatcaata cgcttgaagc ttcgtttaac gtaccaggat gtcgatgaaa aaaataggca
      361 ccgaaataaa cagaaaacag taagccgcaa agagaagaca aaatcaaaaa aaacggattt
      421 tcttcggctt ctgtgatatc agaatgatcc aaaagcaacc agcctaatgc actagaaggc
      481 gcatgtgagg tcttaaggta tgaataacat agaaaaaata agatcgtcaa agttgcaaaa
      541 aaagagccta aatgtaaaaa attagtggtt ctacgttgaa tttccataat atct
//
LOCUS       DACESX010000309          578 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.316, whole genome shotgun sequence.
ACCESSION   DACESX010000309 DACESX010000000
VERSION     DACESX010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.316"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(67..297)
                     /locus_tag="IXF88_002838"
     CDS             complement(67..297)
                     /locus_tag="IXF88_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1509494.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          192 a          101 c           90 g          195 t
ORIGIN      
        1 gattcttata gacagtttgt ctcataacat tgaagtattt tgcaatttta gcattgaaac
       61 acttttttaa tcttcaacgt tgttcttata aacaagatga ttgggaatct tttgaattaa
      121 cactgtatag cttagaacac cctttataga aaacattcgc ttagcaatct taataattcc
      181 ttgtgcccga tgtcgtttag attcagtagg ttcctgctca tcgatcatgg aaaaaatttg
      241 aaggatcaga tccttaataa atttatttaa gagaggatga tccattgctt catccatact
      301 cgataaacta gtgataatga gctacacgtt tttctttcaa gttgatcaat agcttccacg
      361 ataaaaatat ctttagataa aatcaagaac cgtaggaact acagggatat ttggtcaata
      421 agagccaccg ctattagtaa aaaataagct atcaagtatg ctctcatccc aagaattgcc
      481 tacttcaatc gttaaaaacc gttaggttta gctaagtggg ggtagttcac tcaatacttt
      541 tatcaataat agagcgcaaa actttttctc ttaattca
//
LOCUS       DACESX010000310          570 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.317, whole genome shotgun sequence.
ACCESSION   DACESX010000310 DACESX010000000
VERSION     DACESX010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.317"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..>570)
                     /locus_tag="IXF88_002839"
     rRNA            complement(<1..>570)
                     /locus_tag="IXF88_002839"
                     /product="23S ribosomal RNA"
BASE COUNT          121 a          181 c          131 g          137 t
ORIGIN      
        1 acagcccaac ccttgggacc gactacagcc ccaggatgcg acgagccgac atcgaggtgc
       61 caaacctccc cgtcgatgtg gactcttggg ggagataagc ctgttatccc cagggtagct
      121 tttatccgtt gagcgatggc ccttccatgc ggaaccaccg gatcactaag cccgactttc
      181 gtccctgctc gacttgttgg tctcgcagtc aagctccctt ctgcctttac actcttcgaa
      241 tgatttccaa ccattctgag ggaacctttg ggcgcctccg ttacctttta ggaggcgacc
      301 gccccagtca aactgcccat ctgacactgt ctcccaccac gattagtggt gcgggttaga
      361 gtggccataa cgcaggggta gtatcccacc agcgcctcca tcgaaactag cgttccgatt
      421 tctacggctc ctacctatcc tgtacatgcg gtacagacac tcaatatcaa actacagtaa
      481 agctccatgg ggtctttccg tcctgtcgcg ggtaacctgc atcttcacag gtactaaaat
      541 ttcaccgagt ctctcgttga gacagtgccc
//
LOCUS       DACESX010000311          566 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.318, whole genome shotgun sequence.
ACCESSION   DACESX010000311 DACESX010000000
VERSION     DACESX010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.318"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            32..>566
                     /locus_tag="IXF88_002840"
     CDS             32..>566
                     /locus_tag="IXF88_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509495.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELR"
BASE COUNT          220 a           70 c           97 g          179 t
ORIGIN      
        1 atccaacata caatttaaga ggtataagta aatgatgact atagacgaag caaaacaatt
       61 agcaacttat ttttcagttg gttcagcggg atatatttta gactttacta atgtaacgtg
      121 gaacatattt acaaagaaaa tcgtagggat tgaaatacaa aatcattatg ggggctcaaa
      181 gggagtatcc tttcaaaagt ttctgcttga cgatcaattt gatgattcgt taaagaatcg
      241 gttaatccaa tctttaaaag gtattatgga tcgagataaa aaatatgaat ctactgaaat
      301 gaatcccata gttcagttgc tttttgagga agtatttaaa gaagatactt caactgcatt
      361 aagaaaaatg aaattttcaa gcgagattga tttactttat attcggcagc tacccattag
      421 gattcaaaca gatattaaaa acaaggattt tgactctgtt ttgacaaaag caaatacttt
      481 aatagatgaa gttctaaaat ttattattga aaataccgaa ggaccgagtg ttaatattaa
      541 tttatttaag tcaaaagaat taagaa
//
LOCUS       DACESX010000312          563 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.319, whole genome shotgun sequence.
ACCESSION   DACESX010000312 DACESX010000000
VERSION     DACESX010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.319"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            20..>563
                     /locus_tag="IXF88_002841"
     CDS             20..>563
                     /locus_tag="IXF88_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HAQ1509496.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNE"
BASE COUNT          213 a           72 c          114 g          164 t
ORIGIN      
        1 gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg gtagaaatcc
       61 caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg ctagagaact
      121 agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg atttaagttt
      181 aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt atgttctaat
      241 tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg ttgggtatga
      301 aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat ttatcccaat
      361 aattaaagaa ggacgtgcaa gcgaagtact tcctactttt cttgctggaa agcttggaat
      421 tgatttatca gatagccagc attatgatga aaacttagat gatttaatta caactatatt
      481 tggtgttaag agaaaaccag cattaggtga tatacctagg catattaaag gcagtccttt
      541 actacctata gaacataatg aaa
//
LOCUS       DACESX010000313          563 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.320, whole genome shotgun sequence.
ACCESSION   DACESX010000313 DACESX010000000
VERSION     DACESX010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.320"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
BASE COUNT          195 a           98 c           85 g          185 t
ORIGIN      
        1 ttaaaatatg tgcttttgtt agaaaaatca atacataaat tatcatatct gtatatgtca
       61 tttaggtatt tcagttatgt tataatagtt ttgctgtctg ctaccccggc ctaatctggc
      121 aaggggagga ggagatataa tgaaatacct ttttgatttt gtttttgcaa tcaagccatt
      181 tgcagaggta actgcacact acatctgcaa gtggcttgat tgcaaaattg ccaaaaaaga
      241 cagtaagcct accagttaat tctataatat agaaacgtaa aaaaaacccc atcagcctac
      301 acgctgatgg ggtttgatga tataatgaaa tacctctttg ttactcataa ttataccata
      361 attagtcacg aatgcaacca atagaaaaaa ctacttagaa cggtagatca tcgctaataa
      421 caatgcattg tccaccaaat ggattttgtg aagcattaaa tacattgtta ttaattgtcg
      481 ctgtttttaa ctaggcctct ccccttctat tataaaagaa gatatatatt atttcttaat
      541 tacctactac cttaaaccaa aat
//
LOCUS       DACESX010000314          499 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.321, whole genome shotgun sequence.
ACCESSION   DACESX010000314 DACESX010000000
VERSION     DACESX010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.321"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..>499)
                     /locus_tag="IXF88_002842"
     CDS             complement(<1..>499)
                     /locus_tag="IXF88_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1509497.1"
                     /translation="RLDDSPSHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRT
                     RFLCYDCGQTFSAKTDLVDEHHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTR
                     ILREATKHYQPRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYK
                     LTQYFL"
BASE COUNT          143 a          103 c          104 g          149 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata
      361 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aatatctgag
      421 tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
      481 aaggtgagtc atccaatct
//
LOCUS       DACESX010000315          493 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.322, whole genome shotgun sequence.
ACCESSION   DACESX010000315 DACESX010000000
VERSION     DACESX010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.322"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
BASE COUNT          156 a           72 c           88 g          177 t
ORIGIN      
        1 tattacaaat gttacaaata aatagctatt attccggagt ttttagatta aaagtttatg
       61 aaaacaatca caaacgtagg ttttaaagtc ataatcgtta gatattttag ttatttttca
      121 ttcaaaaaaa tagggaacta gtgtttgcat atgtgctttt ttgagttata ataattcatg
      181 aagagaggta attcacttat ttccccaagc tgaaaagcct gatattactt cttttcaggc
      241 agtgctgcta atattcaatt acactttttt gtatattttt agttgtttta aatttaattg
      301 tctgatatgc ttgtgatgaa ttgattttcg ttctgcactt ttggacgata aattgtgata
      361 attgaaaata caaaaaaaag aaaggcccct gtaaggctcg ggagactggt aatctccgtc
      421 agtgtcctac aggaaagcat taagatgacg ccttaatgcg tgcccttcag tgttacggta
      481 ttacccgcaa cgg
//
LOCUS       DACESX010000316          491 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.323, whole genome shotgun sequence.
ACCESSION   DACESX010000316 DACESX010000000
VERSION     DACESX010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.323"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            87..>491
                     /locus_tag="IXF88_002843"
     CDS             87..>491
                     /locus_tag="IXF88_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1509498.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMS"
BASE COUNT          189 a           91 c           78 g          133 t
ORIGIN      
        1 taaatgaaaa aatccataca aaaaaggaag tcccttctgt agaataaagt taacgacaac
       61 caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc
      121 tagtcactaa aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca
      181 cactcctacg gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt
      241 ttaattcagg taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg
      301 aattaacatt ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag
      361 cctacaaaag aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg
      421 ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac
      481 aaaccatgtc c
//
LOCUS       DACESX010000317          490 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.324, whole genome shotgun sequence.
ACCESSION   DACESX010000317 DACESX010000000
VERSION     DACESX010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.324"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..458
                     /locus_tag="IXF88_002844"
     CDS             <1..458
                     /locus_tag="IXF88_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1509499.1"
                     /translation="IITYEFLYNFVFSGGAGCLFALAILTFFFSKSQENKTLGKLSIA
                     PVSFQVAEPLLFGFPTILNIKMLIPFVTAPVVTTLITYFSMSMGLVAKPVGATIPWTT
                     PPIIAGFLASGGRISGAVIQVITIAINVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT          162 a           76 c           79 g          173 t
ORIGIN      
        1 acattataac ttatgaattc ttatataact ttgttttttc tggtggtgca ggttgtttat
       61 ttgcacttgc cattttaaca tttttctttt caaagagtca ggaaaataaa actttaggta
      121 agttatcaat cgctccagta agttttcaag tagcagagcc ccttctattt gggtttccaa
      181 ctatattaaa tataaaaatg ttaattccat ttgtaactgc accagtagta acaacattaa
      241 ttacttattt ttcaatgagt atgggattag ttgcaaaacc tgtaggagca acaatcccat
      301 ggacaactcc accgataatt gccggatttt tagcttctgg tggacgaata agtggtgcag
      361 tgattcaagt aataacaatt gcaataaatg ttttgatata ctatccattc tttaaattag
      421 atgataatgc caagctaaaa agcgaaaaaa atgattagaa ctacctatat gtttctaact
      481 gtagttaagt
//
LOCUS       DACESX010000318          487 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.325, whole genome shotgun sequence.
ACCESSION   DACESX010000318 DACESX010000000
VERSION     DACESX010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.325"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(94..165)
                     /locus_tag="IXF88_002845"
     tRNA            complement(94..165)
                     /locus_tag="IXF88_002845"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Glu,seq:ttc)
     gene            complement(177..250)
                     /locus_tag="IXF88_002846"
     tRNA            complement(177..250)
                     /locus_tag="IXF88_002846"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(214..216),aa:Ile,seq:gat)
     gene            complement(263..333)
                     /locus_tag="IXF88_002847"
     tRNA            complement(263..333)
                     /locus_tag="IXF88_002847"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(299..301),aa:Gly,seq:tcc)
     gene            complement(338..410)
                     /locus_tag="IXF88_002848"
     tRNA            complement(338..410)
                     /locus_tag="IXF88_002848"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(375..377),aa:Phe,seq:gaa)
     gene            complement(414..486)
                     /locus_tag="IXF88_002849"
     tRNA            complement(414..486)
                     /locus_tag="IXF88_002849"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Asp,seq:gtc)
BASE COUNT          152 a          133 c          104 g           98 t
ORIGIN      
        1 cttagcacaa tcatccataa agataactct gctaagcttt ttaaaatcat tctttcagat
       61 aaaaagaaaa agatactcaa aaagtatctt ccatgacccg tacgggattc gaacccgtgt
      121 taccgccgtg aaagggcggt gtcttaaccg cttgaccaac gggccaataa aataaatggg
      181 cctaaatgga ctcgaaccat cgacctcacg cttatcaggc gtgcgctcta accagctgag
      241 ctataggccc ataaaaacta aaagcgggtg acgagaatcg aactcgcgac aacagcttgg
      301 aaggctgtgg ttttaccact aaactacacc cgcataatgg cgcgggacag aatcgaactg
      361 ccgacacatg gagcttcaat ccattgctct accaactgag ctaccgagcc aaacggttcc
      421 gacgggaatc gaacccgcga tctcctgcgt gacaggcagg catgttaacc cctacaccac
      481 ggaacca
//
LOCUS       DACESX010000319          481 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.326, whole genome shotgun sequence.
ACCESSION   DACESX010000319 DACESX010000000
VERSION     DACESX010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.326"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(80..>481)
                     /locus_tag="IXF88_002850"
     CDS             complement(80..>481)
                     /locus_tag="IXF88_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509500.1"
                     /translation="SLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELTPLDRNGI
                     AIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRN
                     GYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
BASE COUNT          150 a           84 c           75 g          172 t
ORIGIN      
        1 tcggtaaatt aattttactt agaatatcgc tatcttgata aaaatttggt gtgttcacaa
       61 attctggcat aaaaaatccc tactttctag ttaaggttcc tgaataagtc gcaatccaat
      121 tgtttccagc tgatttataa ctttgtaaat acaattttcc atagtaccaa taaccatttc
      181 ttttttcttt ccaattccgt gattgaggag gtctagaagg ccaaccaact ttatcaaaaa
      241 tcatcgttcc tgtcactcgt atcgttttag aagctgctcg agcaagtaat ggagaaacta
      301 ttaaataatt ttcagtaaca gttaagtcgt tagttgggtt accaatggct attccgttcc
      361 tatctaaagg agttaattct tggttattat ctactacagt agttggttca tttgtactat
      421 cagcaaatgt aggaattgta gttccagtta cactaattcc aatagttaaa atacctaaag
      481 a
//
LOCUS       DACESX010000320          461 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.327, whole genome shotgun sequence.
ACCESSION   DACESX010000320 DACESX010000000
VERSION     DACESX010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.327"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..>461)
                     /locus_tag="IXF88_002851"
     CDS             complement(<1..>461)
                     /locus_tag="IXF88_002851"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1509501.1"
                     /translation="LMYRSYTEYNDSGILVAIQNDKPIGFLAYSGDMSGLYKYMIKKR
                     IIPFGFYSCVAFIKKPNILMRLFRAFLKPSETKRDENYVELASIGVNPKEKSIGVGSM
                     LIDNLKSRIDFSKYEYIALETDALNNDVVNNFYKKNEFILERKYSTNEGRI"
BASE COUNT          155 a           84 c           48 g          174 t
ORIGIN      
        1 ataattcttc cttcattagt agaatattta cgttctaaaa tgaactcatt ctttttataa
       61 aaattattca caacatcatt atttaatgca tctgtttcta gtgcaatata ttcatatttt
      121 gaaaaatcaa ttctactctt taagttatct ataagcatgg aacctactcc tatcgatttc
      181 tctttcggat taactccaat ggatgctaat tccacataat tttcatcacg ttttgtttcg
      241 cttggtttta gaaaagctct aaaaagcctc ataaggatat tgggtttctt tataaaggct
      301 acacaactat aaaatccaaa cggtattatt cttttcttta tcatatactt atataaacca
      361 ctcatatctc cagaatatgc aagaaatcct attggcttat cattttgaat agcaaccaat
      421 ataccagaat cattatattc tgtgtaagaa cgatacatca a
//
LOCUS       DACESX010000321          460 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.328, whole genome shotgun sequence.
ACCESSION   DACESX010000321 DACESX010000000
VERSION     DACESX010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.328"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(20..91)
                     /locus_tag="IXF88_002852"
     tRNA            complement(20..91)
                     /locus_tag="IXF88_002852"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IXF88_002853"
     tRNA            complement(113..200)
                     /locus_tag="IXF88_002853"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IXF88_002854"
     tRNA            complement(225..298)
                     /locus_tag="IXF88_002854"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IXF88_002855"
     tRNA            complement(312..382)
                     /locus_tag="IXF88_002855"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IXF88_002856"
     tRNA            complement(384..459)
                     /locus_tag="IXF88_002856"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACESX010000322          458 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.329, whole genome shotgun sequence.
ACCESSION   DACESX010000322 DACESX010000000
VERSION     DACESX010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.329"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..351
                     /locus_tag="IXF88_002857"
     CDS             <1..351
                     /locus_tag="IXF88_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1509502.1"
                     /translation="PRETIGVIDKNALSMAVNQPKQHIFGKDLYPDKFSKAAVLLINL
                     IKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVVEFVVEIATYQGDFDDLKEATT
                     DILKKNSTKTDSKK"
BASE COUNT          177 a           67 c           78 g          136 t
ORIGIN      
        1 ccgcgagaaa caatcggagt cattgacaaa aatgcgctga gtatggctgt aaatcaacct
       61 aaacagcata tttttggaaa agatctgtat cctgataaat ttagcaaagc agctgttctg
      121 ctaataaatc tgatcaaaaa gcatccattt cataatgcta acaaacgtac agcattttta
      181 gccgcgtatg tttttctcaa gctaaatggc tattcactaa aaatgaaaaa tcaggaagta
      241 gtagaatttg tagttgaaat tgctacgtat cagggtgatt ttgatgactt aaaagaggca
      301 acaacagata tactaaaaaa gaattcaaca aagaccgatt caaaaaagta gaagcaaact
      361 agggattcag ttaaatctga agatttagtt tgttctttat tttagtttac ataagataca
      421 ttatgcgaag ttcattattt taaaagctta aataataa
//
LOCUS       DACESX010000323          445 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.330, whole genome shotgun sequence.
ACCESSION   DACESX010000323 DACESX010000000
VERSION     DACESX010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.330"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..132)
                     /locus_tag="IXF88_002858"
     CDS             complement(<1..132)
                     /locus_tag="IXF88_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1509503.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          157 a           88 c           84 g          116 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctctcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaat
//
LOCUS       DACESX010000324          439 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.331, whole genome shotgun sequence.
ACCESSION   DACESX010000324 DACESX010000000
VERSION     DACESX010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.331"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
BASE COUNT          174 a           67 c           72 g          126 t
ORIGIN      
        1 gagaaaacat taaccacgat tctaacgcaa acatcaagca cataaaacat ttaacactcc
       61 tcctttctgg ctgaagattt caaccaaaca ggaagacagc gtatcaatta tagcatacac
      121 tttttcttaa attcattttt catatgacaa aatatttcct tctaatcgac aaagttagaa
      181 ggatttttta ttgtctaaat acacaaataa ccttaattta taaagagcat aaggaaacgt
      241 gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc tattacgttt ctcaaaaata
      301 attgtgggaa caaaatattt aaaaatagga tagtgaaagg atagcaaagg atagtaaaag
      361 gatagaagca ggatagcgaa aaggataggc tccttttcgc tatccttttt aaattggagg
      421 aattaatatg gaagtaaga
//
LOCUS       DACESX010000325          430 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.332, whole genome shotgun sequence.
ACCESSION   DACESX010000325 DACESX010000000
VERSION     DACESX010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.332"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..>430
                     /locus_tag="IXF88_002859"
     CDS             <1..>430
                     /locus_tag="IXF88_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ1509504.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DACESX010000326          424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.333, whole genome shotgun sequence.
ACCESSION   DACESX010000326 DACESX010000000
VERSION     DACESX010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.333"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(265..>424)
                     /locus_tag="IXF88_002860"
     CDS             complement(265..>424)
                     /locus_tag="IXF88_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1509505.1"
                     /translation="KIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGL
                     VDNFNNIS"
BASE COUNT          153 a           79 c           42 g          150 t
ORIGIN      
        1 aatcagatta aataatgttt gcttaaaatt gctaccatta atatgactga tagattctct
       61 agcttcttca cataaacttt ttactttatt aattactact ttgtcatatt catttaacac
      121 actattatta ccattactta gacgctcaga ttctacgaaa caactcaaat cagaaatttc
      181 ccaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg taaatgatat
      241 agtctcataa taaaataaaa cctctcagct aatgttgttg aaattatcca ctaaacctaa
      301 ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa agtgtgaaga
      361 atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt tcaacattat
      421 ttta
//
LOCUS       DACESX010000327          401 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.334, whole genome shotgun sequence.
ACCESSION   DACESX010000327 DACESX010000000
VERSION     DACESX010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.334"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..>401
                     /locus_tag="IXF88_002861"
     rRNA            <1..>401
                     /locus_tag="IXF88_002861"
                     /product="23S ribosomal RNA"
BASE COUNT          125 a           87 c          117 g           72 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt c
//
LOCUS       DACESX010000328          395 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.335, whole genome shotgun sequence.
ACCESSION   DACESX010000328 DACESX010000000
VERSION     DACESX010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.335"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
BASE COUNT          129 a           68 c           76 g          122 t
ORIGIN      
        1 ttggacaatt gaaaatacaa aaaaaagaaa ggccctgtga gactcggaag gctggtaacc
       61 ttcgtcgacg tccacaggtg gcattaagac aacctttaat cccttaatgc gtttaccttt
      121 cagtgttacg gtattacccg caacggattt agtataacag atattcaaaa gtgataccac
      181 tttttttggc tgtgttaagg ttaaaaatac gtgatattac gatgaatcta tgcctaagca
      241 taatgttata aaaatgtccg tgtggctctt tgccgtatga cgaacgctga aattagaggg
      301 aacgcatgtt tggatgcgtt ccctcttttt tgtttgtcat gaacgaaaat caattctcta
      361 tttttgaaaa ataacaagaa aaacttatta aaaat
//
LOCUS       DACESX010000329          394 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.336, whole genome shotgun sequence.
ACCESSION   DACESX010000329 DACESX010000000
VERSION     DACESX010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.336"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            65..>394
                     /locus_tag="IXF88_002862"
     CDS             65..>394
                     /locus_tag="IXF88_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAQ1509506.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYE"
BASE COUNT          152 a           48 c           73 g          121 t
ORIGIN      
        1 atatgttttg tgagaaattt agacttacaa taagcagtga atttataatc aacgtatagg
       61 agacatggat gaaatgaaac taattggtat agatttagac ggtactcttt tgaattctga
      121 ccagagcatt agtcaaaaaa atgcaagaac aatgaaacat ttaccggaag actgttttcc
      181 attcatttgt tctggaagag aagtagaaga cattaataat attctcaaaa aatctggttt
      241 aaggatacca gcagtagggt taaatggttc tgttggattt gatggagaaa aaaagatatt
      301 tgagtcttcc ttcgataaaa atgatgtaaa aaaaattgat tctgtcctat cgaaatttcc
      361 aaccaaaata tatacaaatc tcggaagtta tgaa
//
LOCUS       DACESX010000330          391 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.337, whole genome shotgun sequence.
ACCESSION   DACESX010000330 DACESX010000000
VERSION     DACESX010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.337"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
BASE COUNT          157 a           51 c           61 g          122 t
ORIGIN      
        1 tacttttaag agtggttgta tgtaatttta atgcctatac caatttaaag aggatttttc
       61 agattttcac ttacaaattt ataaaaaaac aatataattg atactttgat tttgcgaaca
      121 ttttgccttt ccgcctaatt cagctttaat accttgatta tatcggtaac taatcgcctt
      181 tttagctaat ttaagcatca aataattatc cttaatcagg ttaaaaaata acgaataaat
      241 ggattttggg aataggtaaa aactagatcg gtttctgacg aaaaataaca tgaaaaagga
      301 aatataaaat aacacggagc gtgggaggaa taaagaaatt ttacacacaa agggcgtagg
      361 aatgtaaaga aacttaacac aaaacaaaag t
//
LOCUS       DACESX010000331          385 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.338, whole genome shotgun sequence.
ACCESSION   DACESX010000331 DACESX010000000
VERSION     DACESX010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.338"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..378)
                     /locus_tag="IXF88_002863"
     CDS             complement(<1..378)
                     /locus_tag="IXF88_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1509507.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFG"
BASE COUNT          166 a           69 c           44 g          106 t
ORIGIN      
        1 tccaaataag atatgaaata aatccgttga gctatttgca actcctataa ggataactcc
       61 taatgctaaa aaagaactga aagtaatacc aattgctgta tctcctttta ttacactatt
      121 gctttttata aaagtaataa tgatagaagc taatagacca aatacaatcg caccaataaa
      181 aaaattaata cctaaaatat aagaaattgc tactcctggc aaaacagcat gagaaatagc
      241 atcacccata agagacattc ctcttaaaat aataaaacaa cctactgttc cagaaacaat
      301 ccctataact acagatgtaa ttagagcatt ctgtaaaaag tgaaagtcat acagaccatt
      361 aataaaattt tgaagcatat tattc
//
LOCUS       DACESX010000332          382 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.339, whole genome shotgun sequence.
ACCESSION   DACESX010000332 DACESX010000000
VERSION     DACESX010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.339"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..>382
                     /locus_tag="IXF88_002864"
     CDS             <1..>382
                     /locus_tag="IXF88_002864"
                     /inference="COORDINATES: protein motif:HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1509508.1"
                     /translation="NIVCQIMSLFQNNEKFEMIYVSPDGPIANILNIRGLSYLPLKKF
                     SRKEIDRAVHELNPDIIHAHDFNASVRASRYKKQIILSHLHNNPLWLPKLDIRTIIYS
                     LCSSRFNSVIGVSNSVKEEFRYKQ"
BASE COUNT          141 a           53 c           58 g          130 t
ORIGIN      
        1 aaaatattgt ttgtcaaata atgagtttat ttcaaaataa cgaaaaattt gagatgattt
       61 acgttagccc agatggtcct atagcaaata ttttaaatat tagaggttta agttacttac
      121 cattaaaaaa attttcgaga aaagaaattg acagagccgt acatgaatta aacccagata
      181 taattcatgc tcatgatttt aatgctagtg taagagcaag tagatataag aagcaaatca
      241 tcctttccca tttgcacaat aatcccttgt ggttacctaa actagacata agaactatta
      301 tatattcttt atgttcgtct aggtttaatt cagtaattgg tgtttcgaat tccgttaagg
      361 aagaatttcg ttacaaacaa tt
//
LOCUS       DACESX010000333          371 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.340, whole genome shotgun sequence.
ACCESSION   DACESX010000333 DACESX010000000
VERSION     DACESX010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.340"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..>371
                     /locus_tag="IXF88_002865"
     CDS             <1..>371
                     /locus_tag="IXF88_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509509.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTP"
BASE COUNT          150 a           58 c           84 g           79 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct g
//
LOCUS       DACESX010000334          331 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.342, whole genome shotgun sequence.
ACCESSION   DACESX010000334 DACESX010000000
VERSION     DACESX010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.342"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
BASE COUNT          116 a           64 c           53 g           98 t
ORIGIN      
        1 aaaccactag ctggcgggct agtggttttc ttttacgttc tattcagatt aacgggtagg
       61 cttactgtct tttttggcaa taattttgca atcaagccat ttacagatgt aatgcgcgga
      121 tacttctgct aagattgcaa aaaacatatc gaaaaggtaa ttcataatac aacctcctcc
      181 ccttgccata ataggccggg gtagcagaca gcataaatat tataccataa ctgaaaattt
      241 cattttagta catttcaata tattgatgaa ccagcacaat aaaacatgcc atgactaaca
      301 gaaaaaaact tattaaacca gatataactt g
//
LOCUS       DACESX010000335          325 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.344, whole genome shotgun sequence.
ACCESSION   DACESX010000335 DACESX010000000
VERSION     DACESX010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.344"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(17..172)
                     /locus_tag="IXF88_002866"
                     /pseudo
     CDS             complement(17..172)
                     /locus_tag="IXF88_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
BASE COUNT          109 a           76 c           39 g          101 t
ORIGIN      
        1 tcctaactac tcctttctat tttcttactc cataactaat tgcatccact ggacaagcat
       61 ttttacatcg tccacatctg atacactcca gatgattgca attctcaact gaatctttat
      121 tcatttggca aactttttta cattttccac aattaataca cttatcctta tcctgaatcg
      181 gctacactag actagacaga aaaaataagg tgtgtagaat aataaaaaac acactggagg
      241 atttttcatg ccaagacgtc aacgaagaac ctattcaaaa gaattcaaac aacaaatcgt
      301 cgatctctat ctcgctggta agcct
//
LOCUS       DACESX010000336          323 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.345, whole genome shotgun sequence.
ACCESSION   DACESX010000336 DACESX010000000
VERSION     DACESX010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.345"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(<1..302)
                     /locus_tag="IXF88_002867"
     CDS             complement(<1..302)
                     /locus_tag="IXF88_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ1509510.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DACESX010000337          305 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.347, whole genome shotgun sequence.
ACCESSION   DACESX010000337 DACESX010000000
VERSION     DACESX010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.347"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            53..>305
                     /locus_tag="IXF88_002868"
     CDS             53..>305
                     /locus_tag="IXF88_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1509511.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQ"
BASE COUNT          121 a           52 c           54 g           78 t
ORIGIN      
        1 caaatgtaat tggaaaagcg acttcttaaa caaaattaag gagtcgcttt ttatggcaaa
       61 aataacgaaa aatgagcaag aaaatttggt ggaaaattac acaaaaaaac cggcgtactc
      121 taatagccgg ttagacgcac atttcgtgtg cacttctaat ccgaaattga gctttgacgc
      181 tatgacgata gtcggaaacc ttaataaaaa cagcgctaaa aagctatccg attttatgag
      241 tttagatcca caaattagac tttgggatat acttcaaaca aaatttaaag ctaaagcact
      301 acaag
//
LOCUS       DACESX010000338          298 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.349, whole genome shotgun sequence.
ACCESSION   DACESX010000338 DACESX010000000
VERSION     DACESX010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.349"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..>298
                     /locus_tag="IXF88_002869"
     CDS             <1..>298
                     /locus_tag="IXF88_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1509512.1"
                     /translation="PNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAY
                     QKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKV"
BASE COUNT          126 a           29 c           53 g           90 t
ORIGIN      
        1 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
       61 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      121 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      181 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      241 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtaga
//
LOCUS       DACESX010000339          296 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.351, whole genome shotgun sequence.
ACCESSION   DACESX010000339 DACESX010000000
VERSION     DACESX010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.351"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
BASE COUNT          104 a           43 c           62 g           87 t
ORIGIN      
        1 tggttaaatt gattatttaa gtgggaacat acttgagata tcagctaact tctgtcactt
       61 taaaaaaggg actgtgacag aagtgaaaat agacaaaaaa tatccgaaca agagactagg
      121 atagcagaaa aactatcgtt ttgcaacgag gaccaactgg accgtaggag caattctctt
      181 attgttcgga tatttcgctt gttcaaggac ttatgtctgt agtgcccttt tttggtagga
      241 aaaatggtag ggaaccatat catttttaat gaaaatcaag catttcaaaa gttgaa
//
LOCUS       DACESX010000340          285 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.353, whole genome shotgun sequence.
ACCESSION   DACESX010000340 DACESX010000000
VERSION     DACESX010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.353"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..>285
                     /locus_tag="IXF88_002870"
     CDS             <1..>285
                     /locus_tag="IXF88_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1509513.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNG"
BASE COUNT           97 a           58 c           52 g           78 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acgga
//
LOCUS       DACESX010000341          275 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.354, whole genome shotgun sequence.
ACCESSION   DACESX010000341 DACESX010000000
VERSION     DACESX010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.354"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            11..>275
                     /locus_tag="IXF88_002871"
     CDS             11..>275
                     /locus_tag="IXF88_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1509514.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIG"
BASE COUNT          100 a           34 c           53 g           88 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggtt
//
LOCUS       DACESX010000342          261 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.denovo.355, whole genome shotgun sequence.
ACCESSION   DACESX010000342 DACESX010000000
VERSION     DACESX010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.denovo.355"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            complement(44..>261)
                     /locus_tag="IXF88_002872"
     CDS             complement(44..>261)
                     /locus_tag="IXF88_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1509515.1"
                     /translation="YKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYET
                     PIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           68 a           63 c           52 g           78 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag g
//
LOCUS       DACESX010000343         6566 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.guided.134, whole genome shotgun sequence.
ACCESSION   DACESX010000343 DACESX010000000
VERSION     DACESX010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.guided.134"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..1100
                     /locus_tag="IXF88_002873"
     CDS             <1..1100
                     /locus_tag="IXF88_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1509516.1"
                     /translation="GFYSLFKTLQQNRLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIV
                     PEEAEIIKRIYREYLEGQSLVGIGRALEKDGILTAAGKPRWRPESVKKILQNEKYIGD
                     ALLQKTVTVDFLTKKRVKNEGHVPQYYVENSHEAIIPKELFLQAQEEIHRRSNIYTGE
                     GKNKRIYSSKYALSAITFCGDCGDIYRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRT
                     VKEDELYGAVMTAINKLLAGGNNMIKTLEENIHAVIGETTEYQISEINNSLEEKQKEL
                     IKLANKGQDYDHLADEIDELRDKRQILLVEDASLSGENERINELIEFIRKNKFRTLEY
                     DDKLVRKIIQSVTVYEGHFVIAFKSGIEMEI"
     gene            1243..1467
                     /locus_tag="IXF88_002874"
     CDS             1243..1467
                     /locus_tag="IXF88_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1509517.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            1482..2351
                     /locus_tag="IXF88_002875"
     CDS             1482..2351
                     /locus_tag="IXF88_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1509518.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2332..3066
                     /locus_tag="IXF88_002876"
     CDS             2332..3066
                     /locus_tag="IXF88_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1509519.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGVADEMRRPMMLIVSAKKKM"
     gene            3099..4007
                     /locus_tag="IXF88_002877"
     CDS             3099..4007
                     /locus_tag="IXF88_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ1509520.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4004..4546
                     /gene="sat4"
                     /locus_tag="IXF88_002878"
     CDS             4004..4546
                     /gene="sat4"
                     /locus_tag="IXF88_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1509521.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4639..5433
                     /locus_tag="IXF88_002879"
                     /note="aph(3')-IIIa"
     CDS             4639..5433
                     /locus_tag="IXF88_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1509522.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5541..5729)
                     /locus_tag="IXF88_002880"
                     /pseudo
     CDS             complement(5541..5729)
                     /locus_tag="IXF88_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5709..6281
                     /locus_tag="IXF88_002881"
     CDS             5709..6281
                     /locus_tag="IXF88_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1509523.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         2273 a         1014 c         1479 g         1800 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg gctacagtac cgataccaac
       61 aaggaaaggt gcaggtcaac cataagcgat ttatgggcta cacgaaagat gaagatggaa
      121 atttaactat tgtccccgaa gaagctgaga ttatcaaacg catctaccga gaataccttg
      181 agggtcagag tctagtgggc attggtcgag cccttgaaaa ggatggtatt ttaacagcag
      241 cgggaaaacc aagatggcga ccagaatcag ttaagaagat acttcaaaac gaaaaataca
      301 tcggagatgc ccttctgcaa aagactgtca cagtagattt tctgaccaag aaacgagtga
      361 aaaatgaagg gcatgttccc caatattatg ttgaaaatag ccatgaagcg attattccta
      421 aagagttatt tttgcaggcg caagaagaaa ttcatcggag aagcaatatc tacacaggag
      481 aaggcaagaa caaaagaatt tacagtagca agtacgcttt aagtgccatc accttctgtg
      541 gagattgtgg cgatatttat agacgggtct attggaatat ccacggaaga aaagaatttg
      601 tctggcgatg cgtgactaga atcgagcaag gtcctgaagt ttgtaagaac cgaaccgtaa
      661 aagaagatga actttatggt gcagtaatga ctgcgattaa taagctactt gcaggtggga
      721 acaatatgat aaagacactg gaagagaata ttcatgctgt gattggcgaa accacagaat
      781 accaaatttc agagattaac aactcactag aggaaaaaca aaaagaacta atcaagctgg
      841 cgaacaaggg tcaagactat gaccacttag cagatgagat tgatgagctg agagacaagc
      901 gacagatcct tttagtagaa gatgcctccc tcagtggcga gaacgagcga atcaatgagc
      961 tgattgaatt tatccgcaag aacaaattcc gcaccttaga atatgatgat aagcttgtaa
     1021 ggaagataat ccagagcgtt acagtctatg aaggccactt cgtcatagcc ttcaaatctg
     1081 gcatcgaaat ggaaatatga aaaccagaag taaatagccc atgactctac agtagagttg
     1141 tgggttttct tttgcttgct gattataata atattttaat aactattgtg tttatcaacc
     1201 aaatggttta taatattctt agcgtaaact tggaggtgct gtatgactac ggcagaaatg
     1261 attaaagaac tgtgtgagca aatgaatata agtgtttccg aacttgctag acgtattggc
     1321 cagactccac agaatttcaa taaaaaatta caacgagaaa cagtaacctt ggatgagttg
     1381 aaggccattg ctgatgtgct aggtgtcaag tttgtgcagg catttatttt accagatgga
     1441 gaagaaataa aaatatctaa cgaataaagg aggatgttta tatggtaggt aatgttattc
     1501 aaatagtaac agagaaatta tcatctttgc cttttataga aggcatcgta ttagggggct
     1561 cacgtgcaag aggcacccat acagaaaatt ctgatataga tatcggaatc tattacaatt
     1621 cagactcatt tgacctgaca gcgattaatc aaattgctac agagttggat gatgagaata
     1681 gaaacaacct tgttgtacct cccggagcgt ggggtgattg ggttaatggc ggtggatggt
     1741 tagttataaa cgggtgtcat gttgacttga tattacgtga tataaaacgg gtggaacaaa
     1801 taatcaaaga tacggagcaa ggaattgtta ctgccaatta tcagaccggg catccccatg
     1861 gttatataag tgctatgtat cgtggagaat tagcgattag taaaatacta tatgctaaga
     1921 acgaaagctt atgcgaatta aaaaagcagg cagaaattta ccctactgct ctaaagaaaa
     1981 gcttgatgaa cttttttata tttgaagctg aattctcttt aatgtttgta aaagcaaatg
     2041 cgggggcaga ggataaatat tatattgcag gccatgtttt tcgtattatt tcatgcttaa
     2101 atcaagtact atttgcatgt aataatgctt attgcattaa cgaaaagaaa gctataaaac
     2161 tgcttgaaac ttttgaatat aaacctgaaa aatatgccga aagagttaat catatttttg
     2221 aagtactcgg tctttctctt tttgaatgct acgacatgac cgagaaactt tataaagaag
     2281 tgaatgaaat tgtatcggag ataaataact ttttaaacga ggagagttca gatgaaagaa
     2341 aacaaatatg atgataatat attttttcaa aaatacagtc aaatgagtcg ctcgcagaaa
     2401 ggactggctg gtgcgggaga atgggagact ttgaaaaaga tgctacctga ttttaagggt
     2461 aagcgtgtgc ttgatttagg atgcggctat ggatggcact gtatatatgc gatggaaaac
     2521 ggtgcttcct ctgtagtagg tgttgatatt tctcataaaa tgctcgaagt agcaaaagga
     2581 aaaacccatt ttccacagat tgaatatgaa tgctgtgcca tagaagatgt ggatttccca
     2641 gaggagagct ttgatgtaat actaagttcg cttgcgtttc attatgtagc agactatgag
     2701 aatttaataa aaaagatata taggatgctg aaggctggtg gcaatttagt ttttacagtt
     2761 gaacatcctg tttttactgc tcatggaaca caagactggt attataacga aaaaggagaa
     2821 atactgcatt tcccggtgga caattattat tatgagggca aacggacagc tatgtttttg
     2881 gaagaaaagg ttacaaaata tcatagaaca ctgaccacat atctaaatac actgctttca
     2941 aatagtttta taataaatca gattgtggag ccacagccgc cagagaacat gatggatatt
     3001 ccgggggtgg cggatgaaat gcgacgccca atgatgctga ttgtatcggc aaaaaagaag
     3061 atgtaataat atagaaaaaa taaacgagga gtatgtaaat gagatcagaa aaagaaatga
     3121 tggatttagt actttcttta gcagaacagg atgaacgtat tcgaattgtg acccttgagg
     3181 ggtcacgcgc aaatattaat atacctaaag atgaatttca ggattatgat attacatatt
     3241 ttgtaagtga tatagaaccg tttatatcta atgatgactg gcttaatcaa tttgggaata
     3301 taataatgat gcaaaagccg gaggatatgg aattattccc acctgaagaa aagggatttt
     3361 cctatcttat gctatttgat gattacaata aaattgatct taccttattg cccttggaag
     3421 agttagataa ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta
     3481 gaattaaaag ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa
     3541 gggagtatga tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat
     3601 tgtgccgtaa ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc
     3661 tgctgagaat gatatcatgg aaggtcggca tcgaaacagg ctttaaatta agtgtaggca
     3721 agaactataa gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca
     3781 cctaccggat ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt
     3841 tcagggcggt atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata
     3901 ggaatataac aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc
     3961 tggatagcac atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa
     4021 agcagggcac ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat
     4081 tataccgagg tatgaaaacg agaattggac ctttacagaa ttgctctatg aagcgccata
     4141 tttaaaaagc taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata
     4201 tattgacaat actgataaga taatatatct ttactaccaa gacgataaat gcgtcggaaa
     4261 agttaaactg cgaaaaaatt ggaaccggta cgcttatata gaagatatcg ccgtatgtaa
     4321 ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa
     4381 gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa
     4441 attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
     4501 tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag
     4561 tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa
     4621 agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc
     4681 gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg
     4741 gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc
     4801 tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca
     4861 aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat
     4921 ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg
     4981 tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat
     5041 agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg
     5101 gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt
     5161 ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc
     5221 aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg
     5281 gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa
     5341 gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata
     5401 aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt ctaaaaagct
     5461 ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa ctgtccatac
     5521 tctgatgttt tatatctttt ctaaaagttc gctagataga gttctatatt agagatgtaa
     5581 agagttttgg tgaagaggtt tgtgagtatg gcttttataa ccagggtgca atgagaagca
     5641 ctaacagcag ctagcttaag aacgcatagg atatactttt tatggaagtc acagaggagg
     5701 aataaaattt gaacaggatt aacagagtaa cctctattct tattcagctg caatcaaaaa
     5761 aaataattcc tgccaaagaa attgcacagc gatttaatat aagcttaagg acagtttaca
     5821 gagatatccg gacacttgaa gaagccggaa taccaattgg atctgaggcc ggaaaaggat
     5881 attttcttgt agagggcttc ctacttccgc cggtaatgtt tacagcggcg gaagtgggtg
     5941 cattgattac agcagggaaa tttttaaatt gccatggaga tgaatcattt ataaaggatt
     6001 ttgattcagc tatgtataaa atcaaatcta ttttgaagca tggtgaaaaa aactatgcac
     6061 aggagttgga gaacagtatt aacgtgtaca gcacatctgg acagaaaaat acattggcgg
     6121 ataacgttat tgcagcaatt cagactgcaa tctgcaataa aagggtaata tcaattcaat
     6181 atcctgcatc aggagggcaa gaaccggaaa gcagaatgat agagcctata tcactggggt
     6241 tttatgaaca gaactggtac ttaatcggtt ttgcaggcta agaaatgaat ataggaattt
     6301 tcgtgtggat cggattaaaa gcatttgcat agaagaagag ctctgtcaaa gccatgatgg
     6361 ctctttggaa caaatattaa aacaaatgct ttcatataaa aaactatatg ccctacaatg
     6421 ccgttgtaaa aattggggaa aagggatata ttgattttat atctttctca aaataaatat
     6481 aaaagccctt taaagagggc ttttatatat taatcacaaa tcacttatca caaatcacaa
     6541 gtgattaatc acaaatcact tgtgat
//
LOCUS       DACESX010000344         5438 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.guided.153, whole genome shotgun sequence.
ACCESSION   DACESX010000344 DACESX010000000
VERSION     DACESX010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.guided.153"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            87..1355
                     /locus_tag="IXF88_002882"
     CDS             87..1355
                     /locus_tag="IXF88_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1509524.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            1842..2306
                     /locus_tag="IXF88_002883"
     CDS             1842..2306
                     /locus_tag="IXF88_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1509525.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            2290..2919
                     /locus_tag="IXF88_002884"
     CDS             2290..2919
                     /locus_tag="IXF88_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1509526.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            3273..4751
                     /gene="msr"
                     /locus_tag="IXF88_002885"
     CDS             3273..4751
                     /gene="msr"
                     /locus_tag="IXF88_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1509527.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            4798..5244
                     /locus_tag="IXF88_002886"
     CDS             4798..5244
                     /locus_tag="IXF88_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1509528.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
BASE COUNT         1820 a          931 c         1103 g         1584 t
ORIGIN      
        1 tatttaaata tggacattga cttgctttta cttttttagt tacactgtta tttaggaaaa
       61 gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt agcgttattc
      121 ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg acagaaactg
      181 tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca gagagtactt
      241 tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca actgaaacaa
      301 cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat ccagagaatg
      361 cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc catcaagcag
      421 atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca tctgctaatt
      481 ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca agtgccaatg
      541 acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg gggaacagta
      601 ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat aatggccaat
      661 ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg aatgccgctt
      721 ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta ttgaagacga
      781 cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat acgaactctt
      841 acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat tatggttcta
      901 cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca ccaacttcaa
      961 cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat acattgaatg
     1021 ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt gcttggcaag
     1081 caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga gaccatcatt
     1141 acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt tatgaaggtc
     1201 taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca aatgaaaaaa
     1261 ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca ccgatgggct
     1321 ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa aagctgccct
     1381 gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg tttggttgaa
     1441 cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat
     1501 cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa tattcgacgg
     1561 gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattttct actgcagaag
     1621 agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg tgctttcttt
     1681 atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc tattgagagc
     1741 gtttttttta ttacacagct gattctttga cttttttctt tttttattct attattgaca
     1801 agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta gaagagatta
     1861 gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc tttgaagaaa
     1921 atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa gaaaatccat
     1981 tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt actcgtcatc
     2041 tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag aacaaccggg
     2101 aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat cgagcattgt gaaatggacc
     2161 ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg aaacaatttc
     2221 taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa acgaaagagg
     2281 ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag aagatccgtt
     2341 cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat gatcaaagaa
     2401 gcgacgactg caccttcttc tgtcaatatg caaccatggc gctttgtggt cgctgaaagc
     2461 gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca aaatgatact
     2521 tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc
     2581 tataaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca acaattagct
     2641 gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt agtcaaagtc
     2701 gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca
     2761 aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt ggataaagac
     2821 cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg ttacgagtct
     2881 gttcgtttag caccagaaaa gatcactacg ttcgaataaa aggttttcag ttactattca
     2941 gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa
     3001 tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata aattcaaaag
     3061 acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac gacagtagaa
     3121 taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc agagtaggaa
     3181 aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt atcgataatt
     3241 gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta aatataacaa
     3301 atctacaagt cagctttggc aaccagctag aactatctat tgattctctt cgtgtctatc
     3361 agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact ttgctcaaac
     3421 taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc accttcaact
     3481 acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa ttagccagtc
     3541 atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg gaagaacgaa
     3601 agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta gacgagccaa
     3661 caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt tattattatg
     3721 gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca tcgaaaatct
     3781 gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc tatctcactc
     3841 aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg aaagagaaaa
     3901 aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt
     3961 ccagtaaaaa gaaaaagcaa caaatcagac cggatcggtt gtcttcctct aaacaaaaag
     4021 acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa
     4081 taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta ccaaaatctc
     4141 ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa agaagtggga
     4201 gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc gcgattgtcg
     4261 gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa ggagaaggaa
     4321 tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat caaatgacat
     4381 ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc
     4441 gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg ttatgtacat
     4501 taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag ccaagtaatg
     4561 tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa gctttagaga
     4621 agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct tactttgtcg
     4681 agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa gtacttacga
     4741 gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg gtacagattg
     4801 cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt gatggtcttg
     4861 gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag tttgttgatt
     4921 ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat cggtgtgctg
     4981 gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca aatgatctta
     5041 actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt cggtgtgatc
     5101 atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg gaaaatgaat
     5161 gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag aagtgtgcta
     5221 aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc cttggcatgc
     5281 tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac gataggtttt
     5341 ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat tgaagataat
     5401 ttgctcttca atagatattt ctagaaaggt tctgttta
//
LOCUS       DACESX010000345         3790 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.guided.173, whole genome shotgun sequence.
ACCESSION   DACESX010000345 DACESX010000000
VERSION     DACESX010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.guided.173"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            127..1248
                     /locus_tag="IXF88_002887"
     CDS             127..1248
                     /locus_tag="IXF88_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1509529.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            1506..3008
                     /gene="lsa"
                     /locus_tag="IXF88_002888"
     CDS             1506..3008
                     /gene="lsa"
                     /locus_tag="IXF88_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1509530.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            3075..3602
                     /locus_tag="IXF88_002889"
     CDS             3075..3602
                     /locus_tag="IXF88_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1509531.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
BASE COUNT         1282 a          640 c          849 g         1019 t
ORIGIN      
        1 tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa cagaagaaaa
       61 tagcattgtt ttgtttgaaa aaagtgaaaa aaagtgtctt acttaagaag aaagtgaggg
      121 atatagatgg atattttggc ggcaatcgat tcatttaaag gaagtgcttc ttcatttgaa
      181 ttaaatcagg cagttctaaa tggttgggaa gaccgtaaag gaagaaaaat caatgtgccg
      241 attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcgg gggaacctgg
      301 cgtacgattg atgggatgga tctcttattt cgtcagcgaa aaatcagtta tttattgacg
      361 aactggcaag gaaaaaatac cgcagtcatt gaatctgcac aagtattagg attagattta
      421 cttgataaac caacggatca aaccatccga cttgcttcta gttatgcctt gggagagctt
      481 atcaaagata cactgcttca agaagtggaa caaatcattg tcactttagg aggaagcgga
      541 tgtagcgatg gtggtctagg attactccaa tctttgggtg cttctttaga agggtacaca
      601 gaaggaaatc ctttgttatc tactagaaaa cttcatttgg atcgaaggaa cgttcttgtt
      661 tctaaagtaa aagtgatcat cgctgcagac gtgaccaacc catatacagg tcaacaagga
      721 gctgcttggc aatttggaag acaaaaagga ggaacggata cgacgttagc tttcttggat
      781 aaacaagctg aaaagattgc tcagcagata aaagatgttt gggaggtgga tttagatgac
      841 atcccaggaa gcggagcagc aggcggtatt ggcggagccc tttttttgtt aggagcgaag
      901 atggtctctg gttttgagtt ggtcagccaa atcactggat tagaaaaaca gatcgaacaa
      961 gcggatgtca tcattacagg agaagggcgg attgatcagc aaacaatcca cggaaaagtt
     1021 ccatatggcg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc tatttgcgga
     1081 agtagagacc gagagctgga aggactcttt gattttctcc ccattgtctt aagcattcaa
     1141 ttaggaccaa tcgatttaga ggaagcaatg gaacacaggc aaacattaga aaaaacagaa
     1201 atcttaggac agacactcag cagatttttc aacattctcc cgaagtagtt gacaaaaaca
     1261 atttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg tgcattgaaa
     1321 aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca agggagcagt
     1381 gtgcagattt tcgggattgg cttggttctt tcgatgtatg ggtctttttg tgtgccccaa
     1441 cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta cgaggtgatg
     1501 gaataatgtc taaaatcgaa ataaaaaatc tgacattcgg ctatgacagc caaggcacat
     1561 tattatttga acaagccaat ctaaactttg acacacaatg gaaactagga cttatcggac
     1621 gaaacggtcg aggaaagaca actttactga atatcctgca aaacaaactc ccttatcaag
     1681 ggcaagtgat ccatcagcaa gcgtttgtct atttcccgca gcaaacaaaa gataaagagc
     1741 gtttgaccta ttatgtgtta aatgagatta tggattttga gatatgggaa attgaaagag
     1801 agctccaact gatgcaaaca gatcctgaaa tcttatggag agaattcagc acactatcag
     1861 gaggagagaa gacaaaagtc ctactggcac tcttatttgt ggatgacact catttcccgt
     1921 taatcgatga accaacgaat catttggata tctctggcag aaaacaagta gcggcttact
     1981 tgaaaaagaa aaaacaagga ttcatcgtgg tgagtcatga tcggggattt atcgatgaag
     2041 tagtggacca tgttttagca atcgaaaaga gccaactgga actttatcaa gggaatttct
     2101 ctatctatga agaacagaaa aaacttcgtg atgaatttga aatagctaaa aatgaaaaat
     2161 tgaaaaaaga agtcagcagg ctaaagaaaa cagcagctga aaaagcagaa tggtctcgtt
     2221 cccgagaagg ggacaaaaca aagaaaaaag tcggattcat cgatacggaa tctaggcgag
     2281 tgaataaagg agcagtaggc gctgatgctg cacggacgat gaaacgatct aaagcaatcg
     2341 tgaatcggat ggaaacccag atcagtgaga aagaaaaatt attaaaagat atcgagtata
     2401 tcgatgcttt gacgatgaat agccaagtgt ctcatcataa acggctgtta agcgtagaaa
     2461 atcttcaatt agggtatgaa aatctgttat tcgagccagt tcattttacg atcgaacctc
     2521 atcagcggat ggcaatttca ggtcctaacg gcgcgggaaa gtcatccatt atccattatc
     2581 ttctggggac attcaacggc aaggttgtag gagaaaaatc ccagcccaaa catttgacca
     2641 ttagttacgt acgccaaaat tatgaagaca atcgagggac attggcggaa ttcgcgaaga
     2701 aaaaccgagt agactatcaa gcatttttga ataatctccg aaagcttggg atggaaagag
     2761 atgtttttca taataaaatc gaacagatga gtatgggaca acggaaaaaa gtggaattgg
     2821 ctaaatcttt atcccaacca gctgagctct atatatggga tgaaccattg aattatttgg
     2881 atgtcttcaa tcaagaacaa ttagaacaac tgattttgaa cgtgaaacct gctatgttac
     2941 tagtggaaca tgatcaaacc tttctagata aagtatctac tgagattatt tctcttgaga
     3001 aaatctaaac cttgtaatct caaagacaac aaggtatggt taaggagaac tttgtcaacg
     3061 aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg aacgcacaag
     3121 gattagtttg cgggcgagca gatgtgccgc tgactgaaaa aggacagatg caggcacagg
     3181 cactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc cattcccctc
     3241 ttcaacgagc aagagataca gcacaagcag tagttgatcg tctcaatttg ccgatgaccg
     3301 ctgatgagcg attggtagaa atggattttg gggattatga cggactgcca agcaaagatg
     3361 aaaactttca aaaagcgcgt ttagcgtttg ctgtacgttt cccaaatgga gagtcggtct
     3421 tagatgtgta cgcacggatc gttcctttat tgaaagaatg catggaagac gaagaaaatg
     3481 tctacttgtt agtctgtcat aatgccttga taagagtgat caatgcttat tttcatccaa
     3541 tgcctaatga agggttcttt acattcatgg tagacaatac agaattaatt tcttatgagt
     3601 aattagactt ttgtgttatt taaataaaat gtttcaaggc actgcttttg cttagcagtg
     3661 ccttttttgt ggctgtgtca attcttttgg cattataaat ccaattttta gaaataaaat
     3721 ttaattattt taacaaaagt attgaaattt tgtttcttta gatatatact ctagtcatag
     3781 aaatgaagac
//
LOCUS       DACESX010000346         3711 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.guided.175, whole genome shotgun sequence.
ACCESSION   DACESX010000346 DACESX010000000
VERSION     DACESX010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.guided.175"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            <1..1479
                     /locus_tag="IXF88_002890"
     CDS             <1..1479
                     /locus_tag="IXF88_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1509532.1"
                     /translation="WTTDTNAKIILQKLGISELDKKISTLSGGQKKRVSLAQVLIDEP
                     DLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRYFLDRVANRIFELSFGKLYE
                     YKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRAGVQARGTKQQARINRFEDL
                     KENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQTLLEHLDLLIQSRERLGIT
                     GKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQENEEMAPDKRMIAYLQEAAE
                     EAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKRRLFLLKLLIQQPNVLLLDE
                     PTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVADKLLVFQGNGKQELYYGNMS
                     SYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEWETIEDEIAELEEKISLLQE
                     EMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEWVED"
     gene            1491..2438
                     /locus_tag="IXF88_002891"
     CDS             1491..2438
                     /locus_tag="IXF88_002891"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1509533.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            2458..2985
                     /locus_tag="IXF88_002892"
     CDS             2458..2985
                     /locus_tag="IXF88_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1509534.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3048..3701)
                     /locus_tag="IXF88_002893"
     CDS             complement(3048..3701)
                     /locus_tag="IXF88_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1509535.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
BASE COUNT         1316 a          657 c          787 g          951 t
ORIGIN      
        1 tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag tgaattagat
       61 aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc gcaagtgttg
      121 attgatgaac ccgatttact attactagac gaaccaacaa accatttaga ttatgaagcg
      181 atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat ggttacccac
      241 gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt tggaaaatta
      301 tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg tgatcgtgta
      361 gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc ttggatgagg
      421 gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt cgaagatcta
      481 aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct agcgacacaa
      541 cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa tgaccaaaca
      601 ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat tactggtaaa
      661 aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc cctggatagc
      721 ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga aaatgaagaa
      781 atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga agcaaaaaca
      841 gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt tccaagattt
      901 atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt atttttgttg
      961 aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa tgatttggac
     1021 atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc tgtgatcact
     1081 gtttcccatg accgttattt cttggacaag gtagcagata agttattagt attccaagga
     1141 aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa gcaaaaagag
     1201 acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc tgctgaaaag
     1261 aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga gattgcagaa
     1321 cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga taatttcact
     1381 cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga agaaaaaatg
     1441 gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg atggaacaag
     1501 cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa gacagaacag
     1561 gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa aaagggtttc
     1621 ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg ctttggtttt
     1681 taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt tgggatgaat
     1741 gggcatttga acgctacatc aaaagtccag actataatgg accagatatg actgattttg
     1801 gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag agcaagcttt
     1861 tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat cttggacaca
     1921 tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc gatcaaatca
     1981 aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc gtttctgcat
     2041 ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg tttcaatttt
     2101 atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat gtgtttctag
     2161 gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca catgaaacag
     2221 gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat atcaatcata
     2281 tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact ttagttttga
     2341 atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta gaaggatatg
     2401 acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga tgagaaaatg
     2461 ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga ccgtcttcct
     2521 tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa tacactagtg
     2581 atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg tcagacgatt
     2641 gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca tagtgtggaa
     2701 gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg agggtcagct
     2761 gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat ccatcatgta
     2821 ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact gatcaatact
     2881 agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac gtatcagcgt
     2941 aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag ctatggatag
     3001 actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta agtaaagaat
     3061 aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca aacaacatgc
     3121 atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga aagcccgcct
     3181 gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac agcagccacg
     3241 acacagagcc accccataaa aatatagatg atcgtagata cttttgatac tctctcccgt
     3301 ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca gattaataca
     3361 aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt ataacttcct
     3421 gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt tgcttttgta
     3481 aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat cgatccatag
     3541 atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac taaaagaacc
     3601 actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt aaacacttca
     3661 tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc t
//
LOCUS       DACESX010000347         2769 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.guided.199, whole genome shotgun sequence.
ACCESSION   DACESX010000347 DACESX010000000
VERSION     DACESX010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.guided.199"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            108..191
                     /locus_tag="IXF88_002894"
     CDS             108..191
                     /locus_tag="IXF88_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1509536.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            316..1053
                     /gene="erm(B)"
                     /locus_tag="IXF88_002895"
     CDS             316..1053
                     /gene="erm(B)"
                     /locus_tag="IXF88_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1509537.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1316..2179)
                     /locus_tag="IXF88_002896"
     CDS             complement(1316..2179)
                     /locus_tag="IXF88_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAQ1509538.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2181..2453)
                     /locus_tag="IXF88_002897"
     CDS             complement(2181..2453)
                     /locus_tag="IXF88_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1509539.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2470..2679)
                     /locus_tag="IXF88_002898"
     CDS             complement(2470..2679)
                     /locus_tag="IXF88_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ1509540.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          487 c          443 g          988 t
ORIGIN      
        1 atttgatagt agatttaaaa ctttgcaaca gaaccaaaat tttgtataat aggaattgaa
       61 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
      121 aaatgcgtaa tgtagataaa acatctaccg ttttgaaaca gactaaaaac agtgattacg
      181 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
      241 agataactaa aattacaaac aaatcgttta acttctgtat ttatttatag atgtaatcac
      301 ttcaggagtg attacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
      361 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
      421 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
      481 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaacact
      541 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
      601 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
      661 gaaagccatg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
      721 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
      781 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
      841 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
      901 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
      961 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     1021 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     1081 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     1141 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     1201 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     1261 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     1321 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tagctgcaag ctttccattt
     1381 tttgttgaat tgctttaaat tcaggggttt cttggtgttg attttgaacc attttttgct
     1441 ctattctctc caaagtcggt tgaatttctt tccctgatac tttacgattt aattctcttt
     1501 ctaaggtctc tttcggacta atggaaggcg tttctaagct tgaatagagt ttcactcctt
     1561 ctctattgta aagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     1621 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgcccta gctgtcattg
     1681 gatcatctgc atacatggtt tcatatcgtt caatcgttcc taagtatgat tcaattttcg
     1741 gtactgccat gacgtatatt tttgtttcat aacctttaga ttgaagcatt gtggctgttt
     1801 gaatgggaac gtctgttgtt cgtcctgttc cttcaatcac taaattatag ccttgatcgc
     1861 ttaaacggct aataagtgct tctgtcatgc gattcgaata aggggtaacg tgttttacta
     1921 cgtctttttc atagagtttc actaattcgt caaaatttgg gtgctgttgt ttaaaggtat
     1981 cattatcaat gacaataaca ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     2041 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgctagtc
     2101 tatttttagt tagtcgttca acattctttt ctaaacgatc ttcaaattgc ttttcggtga
     2161 agtcagtaat attcgccatg ttaagccact ttctctttat tcaaaatttg ttttgaatat
     2221 tgattcattg accgccaata ctcatgaata gcttgtaatt cttcaaatgg ttgttgaaat
     2281 aaatcaactt cttgtgccac ttctaattga tttaaaagat tcacttctag taaacgagta
     2341 tttttagtat ctagttcatg attcaaaaca taatttaata cccgattata aacacttgcc
     2401 gataactcat taatgatttc aatttcaaat gttttttcat acgtaactgc catttcattt
     2461 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     2521 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     2581 gtcggcacga acacgaaccg tcttatcccc cattatatct ttttttgcgc tgattggcgt
     2641 atcatttcgt tttgctttct gtgcgcctaa atttcccacg atcgctcact tctttctatt
     2701 tcttcttact atttatttta tcatcaccta tcacaaatca caagtgattt gtgattaatc
     2761 acttgtgat
//
LOCUS       DACESX010000348          881 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Enterococcus faecium 47
            SAMN11579854-rid9592923.guided.290, whole genome shotgun sequence.
ACCESSION   DACESX010000348 DACESX010000000
VERSION     DACESX010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11579854
            Sequence Read Archive: SRR9027827
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000705641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000705641.1
            Genome Coverage       :: 91x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 04:16:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,899
            CDSs (total)                      :: 2,842
            Genes (coding)                    :: 2,777
            CDSs (with protein)               :: 2,777
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 33 of 65
            Pseudo Genes (incomplete)         :: 29 of 65
            Pseudo Genes (internal stop)      :: 17 of 65
            Pseudo Genes (multiple problems)  :: 10 of 65
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11579854-rid9592923.guided.290"
                     /strain="Enterococcus faecium 47"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Austria"
                     /lat_lon="48.30 N 14.28 E"
                     /collection_date="2017"
                     /collected_by="Heidrun Kerschner"
     gene            268..816
                     /gene="aac(6')"
                     /locus_tag="IXF88_002899"
     CDS             268..816
                     /gene="aac(6')"
                     /locus_tag="IXF88_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1509541.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          312 a          128 c          184 g          257 t
ORIGIN      
        1 gttttatttg tcaattcata attggttttt tttaaagcaa aagataatct atatcatgat
       61 gaacaaacag agttttttcc tccaattgat ggctttcctg atctttccta ttttgaataa
      121 agtgtttctg tataattttt ttattttata agtaagataa ataatgaaag aatagagata
      181 ctgattggta gctaaagatt tttgagcgag aacatttcgt tttttcaaaa ggactgttag
      241 aataagcaag aacaaggggg aagacgtatg ataatcagtg aatttgaccg taataatcca
      301 gtattgaaag atcagctttc tgatttactg agactgactt ggccggaaga atatggagac
      361 agctcggcag aagaagtaga agaaatgatg aatccagaac gaatcgcggt agcagcggta
      421 gaccaagatg agttagtagg atttattggt gcaatccctc aatacggtat cacaggttgg
      481 gaattgcatc cattagttgt agaaagctcc cgacgaaaga accaaatagg tactcgatta
      541 gtcaattact tagaaaaaga agtagcttcc agaggaggaa tcacgattta tttaggtacg
      601 gatgatttag accatggaac aacgttaagt caaacggacc tgtatgagca tacatttgat
      661 aaagtggctt ccatccagaa ccttcgtgaa catccgtatg aattctatga aaaattaggt
      721 tataaaatcg taggtgtctt accaaatgca aatggctggg acaaaccgga tatttggatg
      781 gcaaaaacga ttattcctcg accaaattct caataataaa aagaggttgt gacagaagta
      841 atcagcttca agaaataaga aggaatttac gaaaattact t
//
